LOCUS       DACEPW010000001        60526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEPW010000001 DACEPW010000000
VERSION     DACEPW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.001"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            136..1488
                     /locus_tag="IW819_000001"
     CDS             136..1488
                     /locus_tag="IW819_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1243144.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            1659..1946
                     /locus_tag="IW819_000002"
     CDS             1659..1946
                     /locus_tag="IW819_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243145.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            2172..2615
                     /locus_tag="IW819_000003"
     CDS             2172..2615
                     /locus_tag="IW819_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1243146.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(2621..3805)
                     /locus_tag="IW819_000004"
     CDS             complement(2621..3805)
                     /locus_tag="IW819_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1243147.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            4073..4771
                     /locus_tag="IW819_000005"
     CDS             4073..4771
                     /locus_tag="IW819_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1243148.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            4778..5455
                     /gene="nth"
                     /locus_tag="IW819_000006"
     CDS             4778..5455
                     /gene="nth"
                     /locus_tag="IW819_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1243149.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(5581..6114)
                     /locus_tag="IW819_000007"
     CDS             complement(5581..6114)
                     /locus_tag="IW819_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243150.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(6411..8792)
                     /locus_tag="IW819_000008"
     CDS             complement(6411..8792)
                     /locus_tag="IW819_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1243151.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(8782..9429)
                     /gene="recU"
                     /locus_tag="IW819_000009"
     CDS             complement(8782..9429)
                     /gene="recU"
                     /locus_tag="IW819_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1243152.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            9493..10032
                     /locus_tag="IW819_000010"
     CDS             9493..10032
                     /locus_tag="IW819_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1243153.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            10122..10541
                     /gene="gpsB"
                     /locus_tag="IW819_000011"
     CDS             10122..10541
                     /gene="gpsB"
                     /locus_tag="IW819_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1243154.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            10563..10930
                     /gene="rnpB"
                     /locus_tag="IW819_000012"
     ncRNA           10563..10930
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW819_000012"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            11139..12305
                     /locus_tag="IW819_000013"
     CDS             11139..12305
                     /locus_tag="IW819_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1243155.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            12351..13847
                     /locus_tag="IW819_000014"
     CDS             12351..13847
                     /locus_tag="IW819_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1243156.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSCGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            13928..14611
                     /locus_tag="IW819_000015"
     CDS             13928..14611
                     /locus_tag="IW819_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1243157.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            15256..16446
                     /locus_tag="IW819_000016"
     CDS             15256..16446
                     /locus_tag="IW819_000016"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1243158.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            16548..17501
                     /locus_tag="IW819_000017"
     CDS             16548..17501
                     /locus_tag="IW819_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1243159.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            17570..18919
                     /locus_tag="IW819_000018"
     CDS             17570..18919
                     /locus_tag="IW819_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1243160.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(19102..19425)
                     /locus_tag="IW819_000019"
     CDS             complement(19102..19425)
                     /locus_tag="IW819_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243161.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            19554..20717
                     /locus_tag="IW819_000020"
     CDS             19554..20717
                     /locus_tag="IW819_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243162.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            20787..22136
                     /locus_tag="IW819_000021"
     CDS             20787..22136
                     /locus_tag="IW819_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1243163.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(22181..22612)
                     /locus_tag="IW819_000022"
     CDS             complement(22181..22612)
                     /locus_tag="IW819_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1243164.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            23000..25642
                     /gene="alaS"
                     /locus_tag="IW819_000023"
     CDS             23000..25642
                     /gene="alaS"
                     /locus_tag="IW819_000023"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1243165.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            25842..26438
                     /locus_tag="IW819_000024"
     CDS             25842..26438
                     /locus_tag="IW819_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1243166.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            26560..27708
                     /locus_tag="IW819_000025"
     CDS             26560..27708
                     /locus_tag="IW819_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1243167.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(27746..28159)
                     /locus_tag="IW819_000026"
     CDS             complement(27746..28159)
                     /locus_tag="IW819_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1243168.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            28238..28669
                     /locus_tag="IW819_000027"
     CDS             28238..28669
                     /locus_tag="IW819_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1243169.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(28768..29001)
                     /locus_tag="IW819_000028"
     CDS             complement(28768..29001)
                     /locus_tag="IW819_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1243170.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            29184..30854
                     /locus_tag="IW819_000029"
     CDS             29184..30854
                     /locus_tag="IW819_000029"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1243171.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            31001..31387
                     /locus_tag="IW819_000030"
     CDS             31001..31387
                     /locus_tag="IW819_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243172.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            31374..32072
                     /locus_tag="IW819_000031"
     CDS             31374..32072
                     /locus_tag="IW819_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1243173.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            32077..32541
                     /locus_tag="IW819_000032"
     CDS             32077..32541
                     /locus_tag="IW819_000032"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1243174.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            32538..33449
                     /locus_tag="IW819_000033"
     CDS             32538..33449
                     /locus_tag="IW819_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1243175.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            33684..34223
                     /gene="pyrR"
                     /locus_tag="IW819_000034"
     CDS             33684..34223
                     /gene="pyrR"
                     /locus_tag="IW819_000034"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1243176.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            34237..35520
                     /locus_tag="IW819_000035"
     CDS             34237..35520
                     /locus_tag="IW819_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1243177.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            35533..36459
                     /locus_tag="IW819_000036"
     CDS             35533..36459
                     /locus_tag="IW819_000036"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1243178.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            36478..37758
                     /locus_tag="IW819_000037"
     CDS             36478..37758
                     /locus_tag="IW819_000037"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1243179.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            37763..38839
                     /gene="carA"
                     /locus_tag="IW819_000038"
     CDS             37763..38839
                     /gene="carA"
                     /locus_tag="IW819_000038"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1243180.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            38844..42029
                     /gene="carB"
                     /locus_tag="IW819_000039"
     CDS             38844..42029
                     /gene="carB"
                     /locus_tag="IW819_000039"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1243181.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            42083..42859
                     /locus_tag="IW819_000040"
     CDS             42083..42859
                     /locus_tag="IW819_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1243182.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            42856..43788
                     /locus_tag="IW819_000041"
     CDS             42856..43788
                     /locus_tag="IW819_000041"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1243183.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            43785..44501
                     /gene="pyrF"
                     /locus_tag="IW819_000042"
     CDS             43785..44501
                     /gene="pyrF"
                     /locus_tag="IW819_000042"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1243184.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            44508..45137
                     /locus_tag="IW819_000043"
     CDS             44508..45137
                     /locus_tag="IW819_000043"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1243185.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            45125..45439
                     /locus_tag="IW819_000044"
     CDS             45125..45439
                     /locus_tag="IW819_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243186.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            45477..46118
                     /locus_tag="IW819_000045"
     CDS             45477..46118
                     /locus_tag="IW819_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1243187.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            46149..46853
                     /locus_tag="IW819_000046"
     CDS             46149..46853
                     /locus_tag="IW819_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1243188.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            46850..47752
                     /locus_tag="IW819_000047"
     CDS             46850..47752
                     /locus_tag="IW819_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1243189.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(47910..49616)
                     /locus_tag="IW819_000048"
     CDS             complement(47910..49616)
                     /locus_tag="IW819_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1243190.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            49806..50429
                     /locus_tag="IW819_000049"
     CDS             49806..50429
                     /locus_tag="IW819_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243191.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            50879..51928
                     /locus_tag="IW819_000050"
     CDS             50879..51928
                     /locus_tag="IW819_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1243192.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            51925..52806
                     /locus_tag="IW819_000051"
     CDS             51925..52806
                     /locus_tag="IW819_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1243193.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            52809..53579
                     /locus_tag="IW819_000052"
     CDS             52809..53579
                     /locus_tag="IW819_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1243194.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            53858..54712
                     /gene="aroE"
                     /locus_tag="IW819_000053"
     CDS             53858..54712
                     /gene="aroE"
                     /locus_tag="IW819_000053"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1243195.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            54890..56053
                     /locus_tag="IW819_000054"
     CDS             54890..56053
                     /locus_tag="IW819_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1243196.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            56170..56580
                     /locus_tag="IW819_000055"
     CDS             56170..56580
                     /locus_tag="IW819_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1243197.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            56754..58982
                     /gene="topB"
                     /locus_tag="IW819_000056"
     CDS             56754..58982
                     /gene="topB"
                     /locus_tag="IW819_000056"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1243198.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            59120..59437
                     /locus_tag="IW819_000057"
     CDS             59120..59437
                     /locus_tag="IW819_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243199.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            59597..59833
                     /locus_tag="IW819_000058"
     CDS             59597..59833
                     /locus_tag="IW819_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1243200.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            59830..60228
                     /locus_tag="IW819_000059"
     CDS             59830..60228
                     /locus_tag="IW819_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1243201.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT        20019 a        10491 c        12947 g        17069 t
ORIGIN      
        1 gggttcttat gcttgaaaag gaatgaaact acaaacaaac ctttgtgaat tcacaagcat
       61 taaactgttg tatccgtttt atttctgtta tagtagtttt gtcgaaggac aaaatgttaa
      121 tcggagggac cataaatgaa agtagtagta attggctgta ctcatgcagg tactgcagct
      181 gtaaaaagta ttttaaaaaa tcatccagaa gcagaagtga cagtatatga aagaaatgac
      241 aatatttctt tcttatcttg cggtatcgca ctttatgtcg gcggagtagt aaaggaccca
      301 gcaggcttgt tctactcaaa tcctgaagaa ttatcttcat taggagcaac agttaaaatg
      361 gaacacgatg taacagacgt cgatacagaa aataaaacag taacggcaaa agatctgaaa
      421 acaggagaaa cagaaacggt ttcttatgat aaattagtga tgacaactgg ttcatggcct
      481 attatcccac cgattagtgg aatcgatgcg aaaaatgtac tcctatgcaa aaactacaat
      541 caagcaaatg aaattattgc acaagcgaaa gaagcaaaac gtgtagtaat tgttggtggt
      601 ggatatatcg gtattgaatt agtagaagct tttgtcgaat ctggaaaaca agtgacattg
      661 attgatggat tagatcgcat cttgaataaa tatctagata aaccattcac agatatctta
      721 gaaaaagaat tgacagaccg cggtgttact cttgcgctag gcgaaaatgt tactgaattt
      781 atcacagatg aagaaggaaa agtgaaacaa gtagctacac caacagatac atttgataca
      841 gatatggtca ttttatgtgt gggcttccgt ccaaatacaa aactgatcga aggaaaagta
      901 gatacactgc caaacggggc aatcaaagtg aatgaataca tgcagacaag taatccagac
      961 atttttgcag ctggagactc tgcagtagtc aactacaatc caagtggcac gcaaaactat
     1021 attcctttgg caacaaatgc cgttcgtcaa ggtttgcttg tgggaaataa cttgacagat
     1081 cataaaatgg cgtatcgtgg tactcaagga acttcaggac tttacctatt tggctggacg
     1141 atcggttcga caggtgtgac caaagaaagc gcaactttga acgatctaga agtccaagcg
     1201 actgtatttg aagataatta tcgtccagag ttcatgccaa ctactgaaaa agtaatgatg
     1261 gagctagtct atgaaaaagg aacgaatcgt atcgtaggtg ctcagttcat gtccaaatac
     1321 gacattacac aatcagcaaa taccatgtct ttagctgttc aaaataaaat gactgtagaa
     1381 gatctcgcac tttcagattt cttcttccaa ccacattttg accgcccatg gaattacttg
     1441 aacctacttg cacaagcagc gctaggcgaa atcgaaactg cacaataaag aaaaagggtt
     1501 gtgacacaat tttgtcacga ccctctgtct ttttttgtga aaagtacaat tagttgtctt
     1561 tcttcgaatt ttttgtcaca ataagcagca aagataaagc tgttacataa aagaacaagc
     1621 agctttattt gattttgaaa agaaaggtga gaaagaagat gaataaagaa tattttgtcc
     1681 aacaagatgc atttttagaa gattataaaa gtgcttcgaa gaaattaacg atcaatattg
     1741 atgagttggc agatcaagta ctctctcagt tgaatgaaac gcaagaggaa tattacacga
     1801 tgccagcaaa tgacgccaaa gataatgttt ctcatcgttt tcctttcagt agacgcatct
     1861 actcagatga aaatgacggc acattgaata cagagtacac atatgaaggt cagccatatg
     1921 tgctagaaac tgatgatgat ctttaaagac agtttttgaa aaggccgctc atccttaagt
     1981 gatgacaaaa aatcatcaca attaccgatg actgtctctt tactatcttt taatcagaaa
     2041 aaggggttat gacaagcttt gtcaacccct ttttttcttg gtttcagatt gggttttcct
     2101 acaaaatatt tttatctttg ttgaatagga aacaaaaaaa caatataatg aaagaaaaag
     2161 aaggtgtaga aatggataaa agacaactgc taatagaaga atatctggat ttagctaatg
     2221 agattattca tcgtaataaa ccagaaatgg aaaaagaatt tgcaggattt actctaaatg
     2281 aactagaagt gattgaacat atcgggaaaa tccctgatcc taacgtgaca aaattagcga
     2341 atgctagtta catgactaga ggagccatca gtaagctaac aaaaaaactg atagctagag
     2401 ggatcataaa cacttatcaa agcgaagaga ataaaaaaga aatctatttt caattgacgc
     2461 caatcggaca aaaaataagg aatagacacg agcaactcca tcaaaaatgg gtacagcgag
     2521 atgctactgt atttgaaaat atgtcaaaag aagaatttga cacagtattt cgttttatcg
     2581 gtcgatacag agaatttata cgaagtagca aatgaagcta ctatttttta ttgtagtgat
     2641 aataggcaaa aatcataagc aaaaatccga ataccgtcgg aagaaatggt gctgcagcat
     2701 aaagctgtcc tgcgattagc gggccgatga cgcgcgatag agattgtata ctttgggtac
     2761 cgcccattac aagaccttgc tggttatctg agacagaatt ggatagttgg ccattgaata
     2821 caggagtaaa aatagaatca ccgaaaccat aacagataat cccaaataaa aacaaaatcg
     2881 gcagtagaag aattccagat aatgtgatga atagataggc gaggatttca ctgaacatcc
     2941 ctattcttgt gatctgctgt tctgaaaatt ttttgagcaa tcgaggcata atgaacaatt
     3001 gcgagacagt atccaagatt ccaatcaaag agaagcttaa accaaccaac ccagccttcc
     3061 actgaaaggc gtcaatcgaa aattgagaaa agatagattg aagagagcca gctgccaacc
     3121 aaacagcaaa acctgctgtc aataaaggaa taacggatga aatcttgaat aattgttgta
     3181 attggtgaaa aggtcttaca tgggagaaac ttaaatctac tgaacgtttt tttattggaa
     3241 ggctttctag cataaatgtg tagccataaa cggcatttaa gaaagtaaag agtgctccaa
     3301 taaatatagg aacagaattc cccaactcag ctaatagacc ccctataata ggacccagcg
     3361 ttgttcctat accgactaaa gcacccatcc atccaaaaac tttggttctt tcattaggtt
     3421 ccgtgatgtc ggcaaagtaa gcatataagg tggagatttc tcctgccgtc aatccttcaa
     3481 tgatacgacc aaggaacaac atccatatcg aatttcctaa accaaaaagc aaatatccga
     3541 tacttgatcc taataaactg ataataagga tcggatgacg cccaaaatga tcacttaagc
     3601 taccgagaat gggggcagat aagaaggcag ctagagcata aacagacatg agcaaagtga
     3661 tatagaaagc ttgctgatgt atggagtgag aataaggcag cgttaaaaac ggaagtacag
     3721 gactgacgat agtaaatcct aatccagtga ataagacaga gacgtatcca aaaactttag
     3781 aatgtttatt tgtgaaactg tacatgtaaa aactccttta tgcaataatg ttaacttgga
     3841 aacaaaaata gtgtatagta attttgtttc cttgtcaaca ttctaaggaa cagcgaaatc
     3901 tacttaattt atttattccg aaaagtgaag atacgcatca aaaacatgaa caaacaaaaa
     3961 tcaagtagac gacattgtaa atctgaccga tcagtgaagt aaatagaaaa gaaacctgtt
     4021 cttgcttccc atgttttttt tacgtatact gtgattgagc ggaggagaaa tcatgttgaa
     4081 tttagaaaat tacttaagcg ctggacaaac agtcatatct aatcttttac ttaaaaatta
     4141 tcataggatc ggtctacaat ctgaagaatt tttatttgtt ctacagttgc atatggcaca
     4201 gcttgaagga gatactttcc ctgatttgca gatgattgct atggatatgg gaatcaaaca
     4261 agatcagatt tttcaaatac tggatcgtct cgtaacaaat ggatttatca agatagaaac
     4321 gacgattgtc aacggtaaaa aagcagatcg atataatctt taccccatct atgatctatt
     4381 aggtgagtat ttaaaaacac aagaaaaaaa atacgaagaa aaaacacaag aaaaagaaat
     4441 ccagtcagtg tatcaattat ttgaacaaga atttggacgc ccgctttctt cgatcgagtt
     4501 ccagcggatc ggacaatggc tagaagaaga ccattatcag ccggaaattt tgaagctagc
     4561 actgcgggaa gccgtattaa atcaggctta tagttttaat tacgttgacc gcattttgct
     4621 atcgtgggaa agaaaaaatc tacgtacaaa acaacaagtg gaagaagacc aaaaacgaag
     4681 aaaacagcag cttcttcaaa gagaagtaga acagtcagct caacaagaag aattgccgaa
     4741 agtctcactg aaaaattggt tggaggagta gcggaatatg ttaagcaaac aaaaaacaat
     4801 ggaagctcta gaaacgatgt atggaatgtt tcccgaagca catggagaac tcaagcacaa
     4861 taatccattt gagttattga ttgcagtgat cttaagtgcc caggcgacag atgtatcagt
     4921 gaataaagca acgccagatt tgtttgcctc ttttccgaca cccgatgcgt tagcagaagc
     4981 ttcaatcgat gaaatcattc ttaaaataaa aacgattggt ttgtatcgaa ataaagcaaa
     5041 aaacatcaaa gcgtgtgcac agcagctgat tgaacgtttc gatgggcaag tgccaacgtc
     5101 aagagaagaa ttgatgtctc ttcctggtgt aggcagaaag acagcaaatg tcgtattggg
     5161 ggatgcattc gggataccag caatcgcagt agatacccac gtggaacgtg tctcaaaacg
     5221 cctgaggatc tgcaaactag atgctacggt catggaagta gaagaaacac tgatgcggaa
     5281 agtaccacaa gaattatggg taaagacaca tcatacactg attttttttg gtagatatca
     5341 ttgcacggca agaaatccca agtgcgaggt atgtcctttg ctatcaatat gtcaagatgg
     5401 gaaaaatcgg atgcgtctaa aagaaaaggc gctaaaaaag aaaccacgcc tgtagacgtg
     5461 gtttctttaa ttgcaaataa atgtatgaat tatcttgtta ttatggagaa aagctgtttc
     5521 gtttatcttt tttgcatgaa aaagtaaatg tacatactca gtagctagga tggtaactta
     5581 ttacaattta ttgcgataaa aataaaagtt gttattttta ttgggaaaat acccgatgta
     5641 gaaaataatg ccttccaata tacctataaa ataagtccag tattccaaag gattatataa
     5701 ataagtgacg tatctatcta aaggtgtaat aaaaatgata agtgataata aaaaaatacc
     5761 atttataact aaacataaac taatggattt gggcggtttc aggctcatca gaaaaatata
     5821 gattctggtg aacaacaacc ataaaaaagt gagaatcatt gcatgagagc ttctttccat
     5881 aaattttcct ttaagagtag tgcttaaaag tacagaaaaa ataataaatg caaggatagg
     5941 agcaattaaa gtaaatttat ttaataataa acaaaatttt tcagcttttt tattcttgga
     6001 ataacgaggt attgttttcg ataaaaaaag atggtgcaga caaattatga taattaagca
     6061 tactatcaga taactcaaaa tgaagccttc tttcaaaaaa ttattcaaaa ccatatattg
     6121 tattttttat ttgataataa caatgggatt attaagaagt ggcactaaat aaaagactag
     6181 tgtgtattta gccgatttaa ttataaacaa gtaaatggat aaagtatatg taatgataaa
     6241 agaaaaatgg attttctaat ttttaagcta aatgttagat cacacatgta tatgctggat
     6301 agtcttataa aaaatcatgt aatacagctt ggtacatgat ttttatttgt agctgttttt
     6361 tacatagaca caaaaaagaa accacgcttg tagacgtggt ttctttttta ctatctgttt
     6421 ccagaagctg aggtgctgct ggatggtgta tggtcatttg cagcatccgc tccgctagtt
     6481 gctggctcac tggaagaagc aggaggagca gatgaagacg gtgtcgagct ttcttcggat
     6541 gaagaagaag gaaccgttgt tgaagacgct ggctcgcttg tttctgctgt cgtgctttct
     6601 tttgaggact cagcagttga ttcgctttga ctagtagagc tagagctttg agtcgttgtc
     6661 tctgttgttg agcttccagg agtctggaca tagctggaag atggaatagt tgttgatggt
     6721 gtaattgcat tcgatctagc tgtatattga tctttgtagt acagttcacc gcccacacga
     6781 attaatccgc ttggcatctc ccagtctgta tttgtcacgt tagctgaaac atactgcata
     6841 agctcgcgat aaacatcaga ggctacgtgt gacgattctg aagtgacagg tgtcattttt
     6901 ttattgtaac ctgtccacac ggaaatcgaa taatttggcg tatagcccgc aaataggata
     6961 tctggataca ccccagaaga aataccaagt tttgcatact cgtcatctgt gtaattggat
     7021 gttccggtct taccggcttg atataaccca gcgatttgcg cattcgtacc agttccttca
     7081 gtgatcgtat cttttaaaat gtctgtgatc atataagctg tttctggaga catggctgtt
     7141 tttccatctg gctcataagt ttcttctgtt ccgtcttgga agacgatttt attaacatat
     7201 tgaggtttgt aataagtccc tccattggcg aaagccgcat aggctgctgc catttttaag
     7261 gaagaagctc cgtattttgt cccatcttgt tcttccgtat tactagagat cgcattcgat
     7321 tgatggatcg tactgtattc gatcccaaga ttttttaaga aagaagccac tttatctgaa
     7381 ccgacttcat tgaataattt gactgctgga acattacggg ataagtaaag tgcttggcgt
     7441 aaagtaatgg tgcccatata ttgattatcc cagtttccta caggagtcga agtcccttca
     7501 tagttatacg gtgcgtcagt aatagttttt ccagtagaat atttcaaata ttcaaaagct
     7561 ggtccgtaat ctgtgacagg tttcatcgta gaaccaaaat cacgggaagt attcactgct
     7621 aagttgttgc ctaaagttac gtcttctgcg atgtgacgtc cgccaatctg ggcttttact
     7681 ttcccggtat tggtgtcgat aagggtggag gcaacttgca tctcgtcgtc tgggtaagag
     7741 acatactgat ccgtgttgac gatatcatat agtttctttt gggcgtccag atccaaattc
     7801 gtataaatct ctaaaccgtc tgtgtagaca ttcttatctg ttttttcttg tacttcattg
     7861 atgacttctt tgacataatt atcgacgatc ttagtattgt catcggattg agttagttct
     7921 tgcagaccat ccgttaccgg tacattaaca gcttgatcat attctgtttg agagattttt
     7981 tcgttttgaa gcatcgtata caaaacagta tctcgtcttt tcttcgcctg atctggataa
     8041 acataaggat cataggcgga aggggcttga ggcatcccag caagcaatgc tgtttgcggt
     8101 aaagaaagtt ctgataattt cttaccaaag tacatctcag aagctgtctc catcccgtac
     8161 aagccgtttg acatatacac tttatttacg tagtaggtca agatctcttg tttcgatttt
     8221 ttttgttcca atcttacagc catccacgct tcttgggcct tgcgtttcag agtctgatcc
     8281 tcggcactgg tggagaagaa ggaaagtttg atcaattgct gtgtcaaagt acttccgcct
     8341 tgcagtccgc cagaggtaaa gttagagagg gcagaaccga tgatccgaat agggtcaacc
     8401 ccgatgtgtt tataaaaacg gcgatcttct accgacacga tcgcatcttc taatgttttc
     8461 ggtaattcat tcgcagaaat tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg
     8521 ccatcttgtg tatatagttt ggaagatacg gtagcgtcta attttttatc cgtcaattcg
     8581 ggcgcatctt ttgcgtaata ccagaaaagt cctacacctg ctaaaaagag gatacagaaa
     8641 aatgagagta ttcctaaaag gatcttgata aataatcctt ttttatgtgt tccagaggac
     8701 tttccagaac ctttgccaga attcttcttc acactctttt taggagtagg ctgtttctgt
     8761 cgtctagagc ttctagtttg ttcatttgcc attcttgctt tctccttcaa tagataatgt
     8821 tgataaatag aagtcaaccg cgtccaaata aggaatccgc ggagaaatac cagttttgat
     8881 ctctattcct agttcttcga tcaaagataa aggtagtgat ttgcgtcctt tttccttttg
     8941 ggcgtcccag taagcaatca aatgaacact attcaaataa aaacagcgct tcaaagacga
     9001 aaaccataac aagacaaagc agaccccatt ttgttcgagg catgctttca tatgttcgat
     9061 ttgatgggca tggaagtttt taaagggaaa agatgttttg ttcttggttt cttttgcttc
     9121 aaagtccaag taaaagcctc gatagacgcc attatagtct gtagttgagg cttgggtaaa
     9181 gtaggcttcc ttgatcacag cagcacttct cttaggatag tctactttca cgatttggat
     9241 aggggtaggc tttttatgga taaccgccaa cccgcgggaa agataatatt tattactttc
     9301 attgattgct tcttcaaagc gcatcccccg attgccgaat tcagtgatct gatttctttt
     9361 ttcttgaatt ttttcgacgg gagaggcatt cttataataa ggttggccat ttggatagcg
     9421 caataccatc tttaatcaca ctcctaggcc acattatagc aaaaaagcgg atagaaagaa
     9481 aaggaatctg agatggataa gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa
     9541 ctcggtgttt ttaaagaaaa tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa
     9601 gaactccatc agttagcaga agaagggctg gaatgggtac ttgtcagtgg gaatttgggc
     9661 gtcgaactat gggctgtgca aatagtagcg gaattgaaag aagaatatcc ggaactatct
     9721 cttggtattt tgtatccttt ttctgatttt ggtaataact ggaatgaagg gaaccaagaa
     9781 aaaaaacaat tagcagaaca attagcagat tatgtcgaag ctgttagcca tcaaccctac
     9841 caatcaccta gtcagctaaa gaatcacact cgttttatac ttgagcatac agtgggctgt
     9901 ctgctgatct atgatgaaga attccctgga aaaacgaagt atttttttga agatgcacag
     9961 aattaccaag cagatcatcc ctacgaaatt cggctgatca gtatggacga tcttcaaaat
    10021 tttagtgaat aatcgatatt gtgtttgatt tatcaacttt tttcctatac aatgaaaaag
    10081 gtagaaatgt tattacgtaa atgaaaacta gaggtgtaaa tatggcgaat ttggtttata
    10141 gtcctaaaga cattttacaa caagaattca aaacaaaaat gcgcggctat gatcccgttg
    10201 aagttgatga attcttagac aatatcatca aagattatga aacttacagc aaagaactcc
    10261 ttgctttaca agaagaaaac gatcgattga gtgcaaaagt agctcaatta tctaaaactc
    10321 aaggagcagc tcaaacacgc gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca
    10381 attttgatat cttaaaacgc ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg
    10441 atcaacaagc aagtgcagtc aaaccagcac aacctaatcc aaataactac acaaatgcag
    10501 acacaagctt agatgataat gaaaaaacac gtcaatttta atttttgatt aatgaatttg
    10561 cggttttcgg gtaatcgcgg tctgacttga tcaggctgag gaaagtccat gctcgcacag
    10621 gctgtgatgc ctgtagtgtt cgtgcttagc gaaaccataa gctaaggtac tctttggagt
    10681 aacggcagga aaacaagcta aggcttcggc tatgcttaag tatccttgaa agtgccacag
    10741 tgacgaagcg gctggggaaa cccatccggt ggaacgcggt aaacccctcg agcgagcaac
    10801 ccaaacaatg gtaggggcgc tttttgtctg gaagaagaac aggacaaaaa ggcagtataa
    10861 ctgcagatag atggttaccg ctttgttttt tctccctgga aaagcaaagt acagaacatg
    10921 gcttatagaa aaccgcaaac atcagggaat taggtcgtga caaaagtaat gcgttataag
    10981 aaacaagaaa aacatttgaa agctattttt caatacttca gatgaatcgt tcttattaca
    11041 aaacaaactt ttgtatcacc ggttattcgg aatttgagta aaagtgtggg gtaggactga
    11101 tgtcccacct ccttttttta taaataagga gattgtgtat ggaaaaaaac aggaagttca
    11161 acctcgtcgc aactgcggca agcgggctag aagcactggt tggtaaagaa ttaagagatc
    11221 ttggaatcga atgccaagtt gagaacggac gtgcacgttt tgaaggaaca atggaaacga
    11281 tcgctacagc taatttatgg ttacgtacag cagatcgagt aaaaatcgtg gttggggaat
    11341 tcgatgcgta tacgtttgat gaattgtttg aaaacgtaaa agcattgcct tgggaagatt
    11401 ttcttccact agatgcagct tttcctgtag ctggaaaatc gatcaagtcg aaactatata
    11461 gcacacctga ttgccaagcg atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt
    11521 atcaccgtcc agcttctgta ccactgacgg aaactggcgc acattttcag ttggaagtag
    11581 ctttgctgaa agaccatgtg atggtaactc tggatacgac aggaccaagt ttattcaaac
    11641 gtggttatcg cttagaaaaa ggtggagcac cattgaaaga aaacatggca gcagcactta
    11701 ttatgttgac aaattggcga aaagaccgtc cattttatga tcctgtatgt ggttcaggta
    11761 cgctttgtat tgaagcagcc ttaataggcc ataatatcgc accaggattc aatcgtgagt
    11821 ttgcttgcga atcttgggat tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag
    11881 ccgaagaaaa agcagattat gatattgaat tagatattac tggttcagat atcaatggac
    11941 ggatgattga aattgccaaa gccaatgcag aagaaatcgg attaggctct tcgattacct
    12001 ttaagcaaca agcagtcaaa gacttcaaaa cggataaaga atacggggtc atcgtggcta
    12061 atccgcctta cggagaacgt ctaggagaag aagaaactgt tcggaaatta tataaagaaa
    12121 tgggcgatgt attccgtcca ttaaaaacat ggagtaaata tatcttgact agcgacttag
    12181 cgtttgagga attttacgga caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat
    12241 tgcgtacgga tctattccag tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg
    12301 aataatctta ctaattgatt cattcaattg gatgtttgga ggaataggga atgaaagaaa
    12361 aagagttttt acaagaagta aaagaaatca atcttctaca acaagcttta ggaatcctcg
    12421 attgggatac acaaacaggt atgcctgaaa aagctagtgc ataccgaagt gaagtagaca
    12481 gctatcttta cagcatttat ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc
    12541 gctatttttc tgaacatccg gaagaattgt cggaagttgg aactgccgtt tttgccaaag
    12601 taaaagaaga gtatgatttg gaacatcgcg taccggaaga tcttatgaga aaatatacag
    12661 ctgccgtttc ttcagcacac ggacaatggc aaaaagctag agaaacacaa caattttctg
    12721 attttcaaca cgcattgaat caaaatattg aattaacaaa acagttgata ccttactgga
    12781 gaaaagatga aaagacgaat tatgatgttt tgctgaatca atacgaaccc gggatgactg
    12841 tcgaaatcct tgataaggta tttgatcagc ttaaagaagg aattctttcg atccgccgta
    12901 cgctccaaga aaaagggact gaacctgaaa cagacttttt aaatcggaga atgacgaaaa
    12961 aacaacagaa gagatttgta gtgaaagtaa tcgaacaact tggttatgat tttagttgtg
    13021 gtcgtttaga tgacactgtc cacccattta tgactggaat taatccgaat gatgcacgaa
    13081 tcacgacacg atggaacgaa acagatttca aaatggctgt ttttggagtg atccacgagg
    13141 ctggtcatgg tatgtatgaa caagacatcg atgataaata tgcgtataca ccggtttatg
    13201 aaggtacatc aatgggtatc catgaatcac aatcgttgtt taacgagatc attattggaa
    13261 gtagtcgcag cttctggaaa aagcaatatc ctttctttca agaatgtgca gaaggaacat
    13321 tcgatgatat tccttttgac aaattttatc gttcattgaa ccatacaaaa tcaagtttgg
    13381 ttcgtatcga ggcagacagt ttgacttacc ctctccatat catcattcgt tatgaaatcg
    13441 aaaaaatgat tttcaatgaa ggggtagatg ttgctgaact acctgaaata tggaatcaaa
    13501 aatatgaaga gtatctggga atacgaccaa caaatgatgt ggaaggcatc ctacaagacg
    13561 ttcattggtc aggtgcaagc tttggttatt ttccctctta tgcattaggg tacatgtatg
    13621 cggcacaatt acttcattct ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag
    13681 acgattattc tccaatcaaa aactggatgt cagagcatat ccatcaatat ggtgcatcta
    13741 gaaaaccaaa tcaattaatc atggatgcaa cacatgaacc gcttaatccg caatacctga
    13801 tcgactatat gaaagacctg tattttaatg tatatcaggt aaaataaaat acaatttaca
    13861 aagatagctg aaattcatct aatctataga tgttttcagc tatttactta agaaagaagt
    13921 ggaacgaatg gataaattac aagtgatcgc tgattatagt atggaccaat tagcgcatga
    13981 tcaaacagga catgggagcg atcataccaa aagagttgtc aagttagctg aaaaaatttt
    14041 ggagacggaa ccgcaggcag accgctttgt gacgcttgca gcagcttacc tacatgatac
    14101 gattgatgat aaagtcgtta aaaatgagaa cgaggcaaag caacagcttc gtgtattcct
    14161 tcgctctttg ccaatagtag aggaacagat ctcgatgatt ttttccatta tcgaaaatat
    14221 gtccttttct aaaaatctct ctgaagcggc cgaactatct ctggaaggta agatcgttca
    14281 agacgctgat cgtatcgaag cattaggagc aatcggcatt ttgcgtactg cttatttcgg
    14341 cggtggacat gggcacccga tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa
    14401 agaaaattac cgaaaaggca cgacagtgat caatcatttt tatgaaaaac tgttcttgct
    14461 gtctgataaa atgaatactg attacggata tgaagaagcc aaacgaagag aaaccttcat
    14521 gcgtgaattc ttagaagaat tttttctgga atggtctatt gacgatgaaa attttactcc
    14581 agacaattgg atcaaaaata acaaaaaatg atggaggttg tgacagaagt ggtcagctcc
    14641 aagatatttc ggatcatttt ccgtaaaagt tacttctgga acactgttta ccaagaggtc
    14701 gtgagcctgc cgttcagctc cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt
    14761 ttgatgaata ttgtaacttg tgattcggaa ttggcaggag aacaccgttt attcggaaga
    14821 ataggactgt gacaagactt ttgtcacagt ccttttttcg aattcaacaa gtaaaacttt
    14881 taagaaatat tcagagagta aaattaaaaa ttgctgataa acgaagatgg aaattttatg
    14941 tatttttatt ggtctatgca ataaataaat taaatattca gaatacaatt gattttaacg
    15001 aaagggcttc tatcaatggt tgtgttgtat ttatctgccc aggtagagta aaatagatca
    15061 ttatctaaaa ggtaattgtt catactatat aagtcatttg aaagtaaaaa gatactgtaa
    15121 ataaaaaatg tggttaaata attctcataa tcagtgtaag cgttatcaaa aatatagcat
    15181 acttgctttg tagatgaatg acacgatata tgtttggtat gttctgcaag acagatgaat
    15241 atggaggtgg agacaatgtc ttccaaagaa ctaacaatgt atttagagaa agaattggat
    15301 caactgaaag aaaaggggtt atacaatacg atcgacatac tagaaagtga aaatggtgct
    15361 tgtatcatcg tagacggaaa aaagatgatc aatctagcat ccaacaatta tcttggattt
    15421 gccaatcgcg aagaattgaa aaaagcatgt attgaagcaa ccgaaactta tggtgtagga
    15481 gcaggagcag tccgaacgat caatggttcg ctgaaaatcc atcagcaatt agaagaaaaa
    15541 atcgcagaat tcaaagggac agaagcagca atcgcctttc aatctggatt caactgcaat
    15601 atgggcgcta tctctgcagt aatgacaaaa gaagatgcta tcttatcgga tgaattaaac
    15661 catgcatcta ttattgacgg ttgccgctta tccggagcaa aaatcatccg aattaaacat
    15721 caagatatga aagaccttga gaaaaaagca aaggaagcga tagaaagtaa gaaatataaa
    15781 aaaatcatgt atatcacaga tggtgttttc tccatggatg gagacattgc acgattgccg
    15841 gaaattattc cgattgttga aaaatatgga ctgatcacct acgtcgatga tgcgcacggc
    15901 tcaggcgtaa ctggaaaagg tgcaggaacc gtcaagcatt ttggattaag cgacaaaatc
    15961 gatatgcaaa tgggcacgtt atcgaaagct ataggtgttg ttgggggtta tgtagctgga
    16021 tcgaaaacac taatcgactg gttgaaagct agaagtcggc cattcctatt ttctacttcg
    16081 ctaactccag gtgcagctgc agctgcattg gcttcgatta ctttgatgca agaacatccg
    16141 gaacttgtcg aaaaagtttg ggaaaatgct aattatttta aagaagaatt gaaaaaagta
    16201 ggctataaca tcggtatgtc tgaaacacct attactccag tcattttggg agacgaaaaa
    16261 gtgactcaga ctttctctaa gaagctgatc gaacatggga tttatgctaa accaatcgtt
    16321 tatccaactg tgcctcttgg gacaggcagg atcagaaata tgccgactgc ggaacatacg
    16381 agggaaatgc ttgatgaagc agtagcagtt tatcaaaaaa taggaaaaga aatggaaatt
    16441 atttaactaa atttagtttt ttcgtctatt taaaattaga gaacatgttc acgcatatat
    16501 tgttaatcat ctgaagatag tagtaaaaaa gatcagggag gatcatcatg aaaaaagtat
    16561 tggtgacagg atgtttagga caaattggtt cagagttgat cacacggcta cgtcaagaac
    16621 taggcagtga aaatgtcatt gcgacagata tacgcatgcc tgaagtcatt gagaaaaacg
    16681 atatctttga gatcctagat gtaatggatt acgaaaaaat gcgcagcata gcagaacaat
    16741 atcaagtgga cacattgatt catttggctg ctcttttgtc agctgttgca gaagcaaaac
    16801 cacaattagc ctggaatttg aacgtgactg ggctggtcaa tgcattggaa gtcgctagag
    16861 aattccagct gaaatttttt acgccaagtt caatcggcgc gtttggtcca aatactccaa
    16921 aagatcatac cccacaagat actcttcagc gtcctacaac catgtatggg gtaacgaagg
    16981 tagctggaga actactatgt gattattatt atgtgaaata tggcgtagat actcgtggtg
    17041 tccgtttccc tggattgatt tcttatgcaa ccttgccagg aggcggtacg acagactacg
    17101 cagtcgatat ttattattca gcaatcaaag aaggaaagta cactagtttc attgatcaag
    17161 gtacatcaat ggatatgatg tatatgccag atgcgatcga tgccattata aaactcatga
    17221 atgcagatcc tgatcgattg gttcatcgaa atgccttcaa tatatcggcg atgtcttttg
    17281 acccggaaga gatcaaagca tctattcaaa aaatcatacc agattttgaa atggattatg
    17341 atgtggatcc tgtacgtcag tcaatagcag aatcttggcc aaattcacta gatgtttctt
    17401 gtgcgcaaga ggaatggggc ttttcgccga aatacgactt agatgtgatg acaaaggata
    17461 tgttggaaaa actgaaagaa aaattggtag agacggtata aaacaagaac cacatccaaa
    17521 aagtttttta tacttttggg attggctatg gtatgaaagg ggacaaggga tggaagaata
    17581 tcaagccacg ccagtaaaag aagtaaaaat agaaaatgaa ttaaaacgta cgatgggatt
    17641 ttttactgct ttatcgactg ttatgggcac agtgatcggt gcaggggtat tttttaaagc
    17701 cgcaagcgta gcagaagtta caggatccgc cagtttgcat atgttttctt ggtttctcgg
    17761 tggtatgatt tctgtctgtg caggactaac aggagctgaa ctggcagctg ctattccaga
    17821 aacaggaggg atgattaaat atatcgaacg gatttacgga aatacagcag cttttttatt
    17881 gggctgggcg caagtcgtga tttattttcc agcaaatgta gcagccttgt cgatcatttt
    17941 cggtacacag ttcgtcaatc tatttggtct ttcccagtca atgattgttc ccgtagctgt
    18001 tacagctgct gtatcgatcc tgttgatcaa ttttttaggt tctaaagctg gaggggcttt
    18061 tcagtcgatt acgcttgtct gcaagcttat tcctttgttt gtgatcgtca ttttcggttt
    18121 gttccgacaa gaaggagtag acttccaact gtttccgatc caagcaggag aaaatctttc
    18181 atttttctca gccttgggag cgggcttgct agcaacgatg ttcgcatacg acggttggat
    18241 acacgtagga aatatttcgg gagaattgaa aaagccggcg aaagatttgc caaaagcaat
    18301 ttctttagga atcatcggaa tcatgatcgt ttatttatta gtaaatgcgg tgttcttaag
    18361 aaccgcatct atcgacggag tcgcaggaaa cagcaatgcg gcaagtgacg ttgcaaaaat
    18421 gatttttggc ggcttcggtg gaagactggt cacagttggt atcttgattt ctgtttatgg
    18481 aaccatcaat ggctataccc ttactggaat gcgtctccct tatgttatgg caaaagaaaa
    18541 caacctgcct tttagcaagc tctttgctaa acttcatgat aaaactaaag ttcctgtagc
    18601 cgcaggtatc ttagagctag tcatcgctat tggcatgatg atgatcggtg gttttgatac
    18661 actaacggat atgctgatct ttgtcatttg gattttttat acgatggtct ttgttggtgt
    18721 cattcttctt cgtaaaaaag aacctgattt gtttcgacca tacaaagttc caatgtatcc
    18781 gtttattcca ctagtagcaa tcattggagg gacttttatc gtatcaagca cgcttatcac
    18841 tcagaccttt ttagcttcga tggggatagt attgacactt gctggtatcc cgatttatct
    18901 ctatttaaaa cgtagatagc taaaacattc gaattttatt agaactgcac ttctctgcca
    18961 aacaaaatat tttatttaaa tgaggagtac gtggaagaaa tgtcaaaagt actcgttaat
    19021 tttgatcacg taaatgtcgt tttgtctacg atgaaatagc aaagtaaaag acagaaatcg
    19081 ggctcaaccg atttctgtct tttactttga tttgcgtaac tgaactattc ggcgtacgcc
    19141 gcgataaata tttaataaaa ccaagcaacc agaaatgaaa atgggaagcc aaatcagata
    19201 gacagggtag ccaagcactt tagacaaagc atgtggagtc attttatcca tttcaagaag
    19261 tgcttgctgc caagtctgaa gattgaagac tagcatgaaa cccgaaataa tcaggagaat
    19321 aaatcctaaa taatgaattg tagttctttt tcttacataa gcgatcgtag cataaaggaa
    19381 cagtaaaaaa ataggtacgc caaaaacaaa gtacatattc agcatctcga cacccccttt
    19441 gtgtttatct tactttactt tgaaagaaat gagaaataat agcctcgtta cttgcttgaa
    19501 acagcaaagg actgctaaga taagagagat acaaagaatc aaggaggagt catatggcaa
    19561 gttatatcga accgattcgt tttgcgctga ttgtttttcc atttttagcc ttagcgatct
    19621 catctgtttt ttttatttat gaatatcgaa agtatggaac atttctagta acaagggcag
    19681 ttattttgta ttctttcgtt ttttatttat tgtgtgcgta ttttttggtg atcttaccac
    19741 ttccggcaaa ggagacggtt gcccaaatga cagggccaaa gatggagctg catttatttg
    19801 cttcatggca acatttcaga gacaacacgg tattggtctt gtctgaacct agtacctacc
    19861 tgccggcaat gaagcagagt gtatttttgg aaccagcttt taatgtttta ttactcatac
    19921 catttggcat ttatttaaga tattatttcc ggctttcttt tatgaaaacg atgattgcta
    19981 gtttctgcct ttctctattt tttgaattga cgcagctaag tggactttat tttatctacc
    20041 caagaccata ccgcttattt gatgtgaatg atttagtgac aaatacattg ggtggaatca
    20101 ttggttttgt cgttacgccg ttatttacat ttatgcttcc tacaagaaga cgaatggatg
    20161 aactttctta tgaaaaagga aagactgtct cattgactcg acgcttggtc gcttatttga
    20221 tcgactggac atttttatct cttattaatt tggtagcggc gattttcttt aaaattgcgt
    20281 tcgattcagg ggcagttact tctcatatct ggtggctttt attagaagta ggggtatact
    20341 ttatgattgt accgtttata accaatggat ggacacttgg caaaaaaatt gtgcgtatcc
    20401 gtttggtgga agaagaccgc gaacgaattt ctttaaaagc attgatgatc cgctacgggt
    20461 atctgtactt tcttttttat ggtttaagct ggttggggca gttgcttcag caaatcatga
    20521 attcgacaac gaatacggtg ttgcagatga gtgcagtatt catttctctc ggtgtcttgt
    20581 tactacaagg tatttttatt ttaaatatct tgctatcatt tttgagaaaa aaacggattc
    20641 tgttttatga aaaagctagt cacacatata ctgttagcac gattggccaa acaaaggatg
    20701 atgattctcg aaattagctg agcaaaaacg ctttgtctgt gttataatgc caaagtcgtg
    20761 ataatttaaa gagaagaggt gcacgaatga ataattttga tatattccgc ttcaaaccgt
    20821 ttatcaatga agcgttaacc gcaaagggct tcaaacagcc cacagaggtc caggaacgtt
    20881 tgattccttt aattgcaaaa ggaaaaaata tcattgggca atctcaaact ggtacaggaa
    20941 aaacacatac gtttttgttg cctttgtttg aaaaaatcaa tgcagaaaaa gaggaagtac
    21001 aagttgttat tacagctcca agtagggaac tagctgcaca gatctatgaa gcagctacac
    21061 aaatcgctaa attttctaca ccggagatcc gtgtctcgaa ttttgtcggc ggtacagaca
    21121 aacagcgtca attaaatcgt ctgcatcacc aacagccaca ggttgtagta ggcacgcctg
    21181 gtagaatatt agacatgatg aatgaacaag cgctgaagat tcacaccgca tttgcatttg
    21241 ttgtcgatga agcggatatg acattggata tgggtttttt aaatgaagta gaccaaattg
    21301 caagtcgatt accagaaaag ctgcagtttt tagttttttc tgccactatt ccagaaaaac
    21361 ttcgtccatt cttacgaaaa tacatggaaa atccaatcat tgaagaaatc aaaccaaaag
    21421 cagttatttc tgaagacatt gaaaattggc tgatttcaac aaaaggtaaa gatgagaatc
    21481 aaatgattta tcaactgctg acaattggtc atccttattt agcaatcgta tttgccaata
    21541 caaaacagca tgtggatgaa attgccgatt acttgaaaaa ccaaggcttg aaagtagcaa
    21601 agattcatgg agacatcacc ccaagagaga gaaaacgagt gatgcgacaa gtacagaatc
    21661 tggagtatca atatgttgta gcgactgatt tagctgctag agggatcgat atcgaaggtg
    21721 tctctcatgt tattaatgca gaaattccag aagatctaga cttctttatc catcgtgtcg
    21781 gacgtactgg acgtaatggc ttaaaaggaa cggccattac gttatattcg ccaaatgatg
    21841 agcaagccat tgatgcgata gaaaaattgg gcgtctcatt tgaaccaaaa gaaatcaaaa
    21901 acggggaaat cgtcaaaaca tacgatcgta atagacgtgc caaacgagaa aaatcaaaag
    21961 atgtattaga tcccacgctg attggtcttg tgaagaagaa aaagaaaaag atcaagcctg
    22021 gttataagaa aaaaatcgat tgggcaattg cagaaaaaaa taaaaaagaa cgcaaaatcg
    22081 aacgcagaca gcaaagccgt actgctcgta aaaataagaa aaattcacat aaataaaaaa
    22141 caagaggttg tgacaaaagg cgtgtcacaa tctcttgttt ttaagctaga ctttttttca
    22201 ttccgataca aagtacttca tcttcaataa aaggttctgt gcaagtatga tacccttgtt
    22261 tttcataaaa atctacagcc gtcatttcag ctactacata actagtctga caaccttctg
    22321 aaaccgcccg tttttcagca gcttcaagca actgaccgcc taaccctttt cctcggtatt
    22381 cttttgctac gcaaaatcgt tccggcttga agcaagtagg tgttttcttt tcataacgca
    22441 aagtagctac tggtcttttt tgatcaagaa atagataata ggtttgttgc gggatatctt
    22501 ttccgtcgaa ttctagttct gcaggaatcc cttgttctaa tacaaaaact tgatatctca
    22561 aaaagcagct agctgcgcgt atccattcat ccgctccaac tatttttttc atcatattcc
    22621 tccttgtcat gaatagcaga gatgtattga ttcctttatt cttatcgtac caaaaatcgt
    22681 ggagtatgac caaaaggaaa tgaaaataaa aattgagata ttgacagtga tcaatagaat
    22741 gcgtatacta aataagtacg aaaaggatat gatttgtttt ttaagactca cagaaaattc
    22801 tttcttggct gaaaaaagaa tagtcactaa tcaaattgct ccctttttct attttggtag
    22861 aaataccgac gcagctcagc gttatcgagt ttaagaggta atgattacac atcattgcaa
    22921 tcaaggtggt accgcgagtt ttcgtccttg gctgtgatgg tgtttttttg tatttaattt
    22981 atagaaagca ggtaatgaca tgaaacaatt aagtagtgca caagttcgac aaatgtttct
    23041 tgatttcttc caatctaaag gacatacgat tgaacctagt gcgtctttga tccccgtgaa
    23101 cgatccaacc ttattgtgga tcaactctgg tgttgcaaca ttgaaaaaat attttgatgg
    23161 ttcagttgtg ccagaaaatc caagaatcac aaatgcccaa aaatctattc gaacaaatga
    23221 tatcgaaaat gtcggtaaaa cagcacggca ccatacgatg tttgagatgc tgggtaattt
    23281 ttcgattggt gactatttca aaaatgaagc catccactgg gcatgggaat ttttgactga
    23341 tccaaaatgg atggcattcg atccagaaaa actttatgta acagtttatc caaaagatac
    23401 agaagcaaaa cgcatctggc atgaagaagt tggcctgcca ctagatcaca ttattgatgt
    23461 agaagacaac ttctgggata tcggtgcagg cccatgtggt ccggatacgg aaattttcta
    23521 tgaccgtgga gaagcttata acgatttagc agaagacgat cctgaaaact atccaggagg
    23581 agaaaatgaa cgttatttag aaatctggaa cttagtattt tcagaattca accataagcc
    23641 tgataacaca tttgaaccac ttcctcataa aaacattgat acggggatgg gattggaacg
    23701 catggtctca atcgttcaag atgcgtcaac gaactttgag acagacttgt ttatgcctat
    23761 tatccacgcc gtagaaaagc taagcaaaca agtaaaatat ggtgaaaacc cagtaacaga
    23821 tatttcttac aaagtaatcg cagatcatat tcgcgccctt tcatttgcta ttggcgacgg
    23881 tgcattgcca tcgaatgaag ggagagggta tgtcttacgc cgtttgcttc gtcgtgctgt
    23941 catgcatggg aaaaaattag gtattgaaca agcattctta tatcgtttag tccctgttgt
    24001 tggggaaatc atggtaagtt attatcctga agtacttcaa caaaaagaat ttattgaaaa
    24061 agttatccgt acagaagaag aacgtttcaa tgaaacaatc aatgaaggtt tggaaatctt
    24121 aaatcaagtg atccaagaag taaaaaacga aaacgggacg actctagcag gaaaagacat
    24181 cttcaaacta tacgacacat atggcttccc ggtagaatta acggaagagg tggcagaaga
    24241 tgaaggactg aaagttgatc atgctggttt tgaaaaagaa atggatgcac aacgcgaacg
    24301 tgctcgctct gcccgcagta ctgaaaaatc aatgggggta caatctgctt tattgacaga
    24361 tatcaaagta gaaagtaaat tcattggata cacagaacaa gaatcagata gtcaattatt
    24421 ggtcatcgtt aaaggtgagt ctcttttgaa tgaagtcgca gaaggtgaag ctcaaatgat
    24481 ctttgatcaa acacctttct atgcagaaat gggtgggcaa gtagcggatc aaggatggat
    24541 catgaatgaa gtaggcgaaa tcgtagcagt tgttacagat gttcaaaaag cgcctaatgg
    24601 tcaatttatg cacaccgtag aagtaaaagc accaatgagt gaaggacaaa cctatcatct
    24661 attgatcgat caatcgatgc gcaatcggat catcaaaaac catacagcaa cccatctact
    24721 gcacaaggca ttgaaagaag tacttggcga gcatgcaaat caagctggtt ctttagtcgc
    24781 tcctggacat ttacgattcg actttaccca ctttggtcaa atcacttcag atgaattagc
    24841 tgaaatggaa agaatcgtca acgaaaaaat ctgggaagct atcccagttg aaacaattga
    24901 aacagatatc gatacagcca aaaacatggg tgcaatggct ttgtttggtg aaaaatacgg
    24961 acatgacgtt cgagtagtaa atattggcgg ctggtcaatc gaactgtgcg gaggcacaca
    25021 tgtagcaaat acagaaaaca tcggaatctt caaaatcgtt tctgaatcag gaattggtgc
    25081 aggcgtgcga cgaatcgagg ctgtaacaag taaagaagcg tacgaactac ttgaagaaga
    25141 agaaaaacaa ctaaaacaag tagcaacaat tgttaaatca cctcaattga aagaagttgt
    25201 ttcaaaagcg gaacaattgc aacagcaagt aagagagcta caaaaggaaa atgaacaatt
    25261 agctagcaag ttagcgaatc aacaagctgg tgatgtattc aaagatccag aaacgatcaa
    25321 tggaaaaaca atcattgcag cacaagtaaa agtgaaagat atgaatcagc tgcgtcaatt
    25381 agctgaccaa tggaaacaaa aaaatatttc agatattctt gttttagcaa acgaacaaga
    25441 aggaaaagtc agtctgctag cggcaatgac gaaagaatca aatgaagctg gactaaaagc
    25501 tggcgacttg attaaagcaa tcgcaccgaa agtaggcggt ggcggtggtg gacgtccaga
    25561 catggcacaa gctggcggga aaaatcctgc tggtattcca gatgcactta aagctgcgaa
    25621 agaatgggtt gcgcaacaat agggcgttta cgtttgacag aaaaacaaaa gttcaaacat
    25681 tgaacgaaac atgattatgg gggcgaagac aaacaagtct tgccctcata cttttttcaa
    25741 ttggatggcg catctaatca aatggtcgga ttcggatggt ctttagttga cgaaagtgac
    25801 agaaattttt acgattagta taaataaaaa ggggtgaaaa aatgaatagt agaaataaga
    25861 cctttcgact agtcttacgt gcgatcttgt tagcaatcat catcgtacaa gcgatggttc
    25921 cttggctggg gtttattcca ttaggattca ttagtttaac aattatccat atcacagtaa
    25981 ttattgctgc agtagtatta ggcccaaaag atggcatggt gattggcttg ttttggggaa
    26041 tcgctacaat tgttcgtgcc tatgccatgc caacaacgcc atttgataca ttagtgttca
    26101 cgaatccgat tatctcggta gttcccagag tacttgttgg cttagtagca ggcttggttt
    26161 tccactggat ttatcaaaaa aataaatcaa taacgatcag ctcagccttt gcaggggtac
    26221 tgggttcatt ggtcaataca gtcttagtgt taggattcat gggattattt tatacaggtg
    26281 caacggcaga tgcctatggt gtagactctg ctcttttatt taaaacctta gcaggggttg
    26341 cagcaatcaa tgggatacca gaagcaatag gtgcgggaat cataacacca ttactagcaa
    26401 aagcgttatt tgcagcaaca ccgttgaaac cagaataatg accatctttt aatagtaata
    26461 ggacgcacga tagatagaaa aaggaagaga ggagaggaaa acaatcgtcc tctccatctt
    26521 tttacctgag caacaagcaa actagaaggg gtaaaaaact tggctaagaa acaagaagaa
    26581 aaaaagaaac aaataccgca acagcggaca aaaaaaggag tactagcggg tatcttagga
    26641 ttggttacga atctgatctt gtttgtctct aagctaatga tcgggctttt ttcaggaagt
    26701 gtctccatta tggctgatgc agtgaacagt ctatcagata ctgcttcatc catattgact
    26761 ttgatcggat ttaaaattgc tgcaaagcca gcagatcagg aacatcctta tggacacgaa
    26821 cgcttcgaat atatcagtgg gttatttgtt tcgatcatca ttacttatgt aggatttcaa
    26881 tttttagagt cttctgctga aaaaatcatc catccagaaa atgtctcttt aacacccatc
    26941 gtcttttttg tcctaatcat ttccatcttt ttgaaatttg cacaaggcaa aatgtatcag
    27001 gttattgcgc aatccattga ttctgaaaca ttgagagcta cagcaacaga tagttataat
    27061 gatgtcttta ctacactggc tgtattgctt tcagcagcaa tcgaatgggt aactggatgg
    27121 cgtattgatg gatatatagg ttttgcttta gcattatata tcctttatag tggattgacg
    27181 atgcttcgaa attttgtata cgaactatta ggaagtcgcc caacgccagc agagattgaa
    27241 gaaatggaag agcatttaaa tgcttgcgat gatatacttg gctatcatga tttattggtg
    27301 cataactatg gacccaataa acgctttgca tcagtccaca tcgaagtaga tgaatcaatg
    27361 aatttgaatg tggctcataa gattattgat ctgatagaaa aagactttaa aaagtctttg
    27421 gatgtggaac ttgtctgtca cttggatcca atgcctgttg ataacgaaaa atactataat
    27481 atcttgcggc agttaaagca aattcttctt gaaacaggta cagggcttaa tattcatgat
    27541 tttagagtaa ttcatcaagg aaaaacgctg caatttgatg tagtggtccc tgagaaattc
    27601 cagtttgttg atacagaact agaaagaatc ttacgtagtc aaatagaaga aaaaatcgga
    27661 gattaccaat tggaaattac gtttgatcac aattatcttt tatattaaat gagaaactat
    27721 aaaaagactc cttaaagttt ttaatttaaa gagtcttttt ttcttttaat tgtttatgga
    27781 gagcctgacg tgcaagatta tctgctcctt tatttttgtt ttcgggcaac cattgcagga
    27841 tcaataaaga aaattcggat agaagttctt ggattctttg aaggtaggga aggaagagct
    27901 cgttcttcgt tgcgtttttg tcgaccgtag aaactagtgt tttgctgtct gaaaaacaga
    27961 aaatcgtttc attctgtaag tcgtttttct ttagaaattc taaagttttt aaaaagactt
    28021 cgaactctgc ttgatggttt gtccctatag aaagcgggat agatagctgt tcttgtttac
    28081 cttgatagac aatcaagatt ccacctccac taggaccagg gttgcctttt gtagaggcat
    28141 caatgtatac ttttaacata aaaagagatt ctcctttttg ttcaaactaa tgttatggta
    28201 agattatatc attactgaat cggtgagagg ttagagaatg aaaaaaaata ttcgatggca
    28261 gcctgaactg gcacatacga tcatctattg gtcattgacc ttgatcattc tgttttttag
    28321 tctgattctt tctttagaaa acacaggtcc ttattggaaa agcaattttg ttttaggtat
    28381 ttttcttgtt tttctatttc taggtttgcg gcgttctttt attttaaaat caaactctgt
    28441 gattatacgt tatgctgctt tttggaaaga ccgagaaata tctgtcggac agattcgatc
    28501 tttgacgcca actaatactg gggtgcaaat gatgttgcaa gatgagaaag ccccatctgt
    28561 tttcatgatg cgaaaaaaag atcaagtagt cctttttgat cattttgaaa aaaacggacc
    28621 caaatcagaa tcaattcctt atccttatac gaaaaaaaga aaaagctaat tttaaaaatc
    28681 agaacctaaa aaaacgaatt tttacaggaa aagaaaagag tgagacgtac ggttcatccc
    28741 gtttagcgtc tcactctatt tgtttatcta ttcatgcata ctagtatcgg tagtctgaac
    28801 gttgcctttc ttgacatggg cagcttgtgt tcctcgattc ccttcgatga tctcaaattc
    28861 gaccacttga ttttcttcca aagttttgaa tccctcttct tcaatcgcag taaaatgtac
    28921 aaaaatttct tctgtgtcat cataactgat aaagccgtat ccttttttgt tgtcaaacca
    28981 ttttacaatt cctgtggtca aagcactcac gacctttcta aagtctgatc atagccttca
    29041 ttataaagag tatttataag tacgtcaaat aaattaaaaa cctgtaattt tatgtttttt
    29101 ttctgaataa aaaaaggtat aattggcata tcttaacaaa gaagatattt tgttgaataa
    29161 agttcgtgaa aaggggtaat caaatgaaga atgatctgca aatttcacaa gaaacagagc
    29221 tagaaccgat tgttgaagtg gctagtaaat tagatcttaa ggaagacgat ttggaactgt
    29281 atgggaaata taaggcgaaa atcaattttt ctggaatcaa tcgatcaaag gataacgctg
    29341 aggggcattt gatcttagtt acttccatca atccaacacc agctggcgaa ggaaagtcaa
    29401 cgatcactgt agggttagga gacgcgctca ataaaatcaa taaaaaatct gttattgcat
    29461 tgcgagaacc ctcacttggt cctgtgatgg gaatcaaagg tggtgctgca ggcggaggat
    29521 atgcacaggt attgcctatg gaagatatca acttgcattt tactggtgac atgcatgcta
    29581 tcacaacagc taataatgca ttatctgctc ttttagataa tcatattcat caaggaaatg
    29641 aattgaacat cgatgcccgc cgagtgattt ggaaacgggt agtcgatctc aatgatcgtg
    29701 aactgcgtaa agtaattgtt ggcttgggtg ggccaatcca aggtgttcca agagaagatg
    29761 ggtttgatat cacagttgcc agcgaaatca tggctatctt gtgtcttgcg gcagaccttg
    29821 aagatttaaa ggctcgtctt gcaaggatcg ttgtcggcta tacatttgac cgtcagcctg
    29881 tgactgctgg agacttgaaa gcagaaggcg ctctggcatt actgctgaaa gatgcaatca
    29941 agccgaatct tgtacaaaca atttacggaa caccagcttt tgtccacggg ggaccgtttg
    30001 ccaatatcgc tcatggctgc aatagtattt tagctaccaa gacagcattg agattagcag
    30061 attacgttgt cacagaggca ggatttggtg cagatcttgg cggagagaaa tttttagata
    30121 tcaaagtacc taacttgaaa aaagcgccgg atgctgtggt gattgtcgcc actgtccgtg
    30181 cattgaaaat gcatggcgga atgaaaaaag atgaacttaa aaatgagaat cttgatgctt
    30241 tgaaagtagg ttttgcaaat ttaaagcgcc atatacgtaa tatggaacaa tatcagctac
    30301 ctgtgatcgt cgcaatcaat gaattcgtaa cagatacaga caatgaactc acactgttgg
    30361 agcacttgtg tgaaaatcag gggattcttg ccaagagagc gagtgtatgg gcagatggag
    30421 cagaaggcgg cgttgattta gcaaaagctg tagtacgatt gattgataga aaagaagcta
    30481 attacaagcc attataccgc ctagaagaaa cgatacaaga aaagacagaa acaatcgtca
    30541 agaaaattta cggaggaaat ggtgttgttt tcagcaaaaa agcgcaaaaa caaatagaag
    30601 agtttacgaa gaatggatgg gacaaactgc cgatttgtat ggcaaaaaca cagtattcat
    30661 tttctgatga tccttcactg ctaggtgcac cggaagattt caccatcacg atccgcgaaa
    30721 tcatcccaaa attaggggca ggatttttag tagccttaac tggagatgtt atgacgatgc
    30781 ctggtttgcc taaaaaacct gctgcattga atatggacgt gacaaatgac ggagaagtgt
    30841 tgggattgtt ttaagcttga ttataatcta ccatcgaagg agctgtattt tgactacagc
    30901 ttcttttttt agacgatagg gaattaattt tgtttacact aggatcattc tttttcttta
    30961 ttgtattttg agctatacta aagcataata ggagggtatg atgaaaacga catttataag
    31021 aaaaaccggt ttttacggta tgggtagctc gattcagcta agaatcaaca atgaaaaacg
    31081 cagtgttctc agtcatgaac aaaagataac gcttgatctg gaagtgccct ttacgatgca
    31141 ggtcaccttt tattggctaa aaagccccgt ttatacagtg aaagaagcaa agcctgctta
    31201 tgtgatcaca atgaattact tgattcttca aatctatccg tttttatttc tagcaactgg
    31261 gttgggggcc gttttcctac aaaatttatt tttcagtttt tttgcagtta ttgtgttgat
    31321 tgcgttcttt ttctatataa aaaaccgggc atatttgatc aaggaggaaa attatggcaa
    31381 actctgatct ttttttaatc agtttcaatc gaatcgaaaa atggatgaga gacgagatgg
    31441 gaaatgcacg aaatatggga tttaccgaac ttgtcagacg cctagctcaa agaaaacaat
    31501 taatgatcag aaaatacgaa gatgatcttt tacagcttgc tcagttaagg aatgcgatcg
    31561 tccatgatcg gatcgcagtc gattttgtca ttgcagagcc aaatgattgg acagtcaagc
    31621 gaatccagct gatcgaacaa gaactgattc agcctgaaac cgtgctgcct cgatttgcaa
    31681 aacgtgtaac aggttttgaa caagatcttc cgttacttga actgttaaag atagtagcag
    31741 aaaaaaggta ttcacaattt ccgttataca ataaaggaaa atttgtagca ttgattactt
    31801 tacgtaatat cgggttttgg ctagcaaaag aaagccaaaa aggtccggtt gatttaacaa
    31861 ataaacgagc gttggatttg atcatccaaa acggaaagta cacaaattat cattttgttc
    31921 cagctaaaac acatatttac gaggttgaag cgatgtttcg tgagcaaggg actttggaag
    31981 ccgtacttat cactaaagat gggaatcccg atggtaatct gctcgggatc gtacgacctc
    32041 gtgatattta taaacaaata gaaaaggatt gattttttgt taattattta ttggatattc
    32101 agcgctttgc tagtaggagt ggatcagttg gtcaaatggt ggatcactga taatctagcg
    32161 cttggagaga cgaaaaatct tataccaggt attttgtcac tgaatcatat tcgaaacact
    32221 ggagccgcat ggagtatgct agaagggaag atgtggttct ttactatagt gacactcatt
    32281 gcagtcgttg tgattctcac attgatgatt aaaaatcgtg aaaaaggtaa tcgctggttc
    32341 atgattggat tgagtttgat tctagctggt gcgattggca attttatcga ccgtattcgt
    32401 cttggctatg tagtagatat gttccagaca gattttatga gctttccgat ttttaacgta
    32461 gcagatgtga cacttgtatg tggagtgatt tgtatgttaa tttatattat tttagatgaa
    32521 aaggatcaaa gaaaaaaatg acagaactta aaacgacgat tcaccatgaa tctggtagga
    32581 tcgataaagt attgactaat ttattcaccg attacagtcg ttcgcaaatt cagctgtggc
    32641 tgaaaaatgg agcggtttct gtcaatggac agccagtcaa agcaaattac aaagtaaaac
    32701 aaggagacga gattactgtc ttgatccccg aaccagaaac attagatgtg gtcccagaaa
    32761 atatcccgtt gacgatcgtt tacgaagatg aagatgtagc ggtgataaac aaacctcaag
    32821 gaatggtcgt tcatccttca gcaggtcata cgcatggcac aatggtcaat gcgctgatgt
    32881 accatatcaa ggatctttct acgatcaacg gcgttattcg tccaggtatt gttcaccgaa
    32941 tcgacaaaga tacttccgga ctgctaatgg ttgccaaaaa tgaccaggca cacgaatcat
    33001 tagctcagca attaaaagat aagacctctt tgcgcaaata tgttgcttta gtccatggag
    33061 agattcctca cgaaaaagga accatcaatg cgccaatcgg ccgttcgaag gaaaaccgga
    33121 aaatgcaggc gataagagaa gatgggaagt cggctgtcac acattttact gtattggaac
    33181 gtttcgaagg atttactctg gttgaattaa aactagaaac cggaagaaca caccagatcc
    33241 gtgtgcatat gaagtatatc ggttatccgt tagctggaga tccagtatac ggaccgaaga
    33301 aaactttgaa aggcaatggt cagtttctcc atgcgcaaat gttaggtttt acgcacccga
    33361 ctacaaacga aaagatgatc tttgaagcac ctcttccaga agtttttgaa aaaactttag
    33421 aaaaattaag aaaaaacatt gcattttaat ttagataacg gtatagtaat taacaacgaa
    33481 agcataaaac ttaatagaat tcctttaagt ttagtcccgt gaggctaaga agggtgggag
    33541 caaggatacg tatggtgcgc cataagtaga ttgctcattg acatagtata gctatcagtg
    33601 tatcagtata cgctcagtct aaacattcct cctgtcctct tagggcagga gtttttttat
    33661 gcaaaaaagt agggggaatg aaaatgcaag caaaagaagt agtagatcag gtaaccatga
    33721 agcgagcatt gactcgaatt acttatgaga ttatcgagag aaaccatagt atccaagata
    33781 tcgtattggt aggtatcaaa acacgaggaa tctacattgc atcaagaatt gccgaacgcc
    33841 tgaagcagct agaagatatc gatattcctg taggagaact ggatatcact ttataccgag
    33901 atgacaaaaa agaaaatcca gaagaaccgg aacttcattc ttctgacatc ccagtttcct
    33961 tagaaggaaa agaagttatt ttgattgatg atgttttgta tacaggacga acgattcgag
    34021 cagcaatgga cgcagtaatg gattttggca gaccaagaaa aatttcatta gcagtcctgg
    34081 tcgatcgtgg gcatcgagaa ttgccaatcc gtgcagatta tgttgggaag aacattccaa
    34141 catctcgggc ggaagagatt ttagtagaga tgcaagagct tgatggacaa gatcgcatca
    34201 tgattttgaa agaggaagat tagaggagag agaaacatgt cggagaaaaa aggatttcat
    34261 aatgatgacg tggtgttaga tatccacgat cgcccaaccc caggacattg ggtaggatta
    34321 agtttacaac acttgttcac gatgttcgga gcaaccgtgt tagtccccat cttagtaggt
    34381 atcgatccag gtattgcgct agtcagttca ggactaggta cattggtgta cttatttact
    34441 actaaaggaa agatacctgc gtacttagga agcagcttcg ctttcattgc agcgatgcag
    34501 cttttgatga aaacagatgg ttatccagcg attgcacaag gagcagtcac aactggatta
    34561 gtctatttga ttgttgcgct gatcatcaaa aagatcggtt cagcatggct ggataaaatc
    34621 ttgcctccaa tcgtcgtcgg tccagtcatc atggttatcg ggttgggact tgcggcaaat
    34681 gcagcaacaa atgctatgta taatcagggc acgtatgatt tcaagtatat tgctgtagcg
    34741 ctgatcacat taggattaac aattttcttc aacatgtggc taagaggttt tctcggattg
    34801 attccgattt tactaggtat tattagcgga tacatcgtag ccttgctgtt tggaatcgtg
    34861 gatactaagc caatcgtaga cgctgcctgg tttgctgttc ctaactttga gattcccttt
    34921 gtacaatatg aaccaaagct atatcttgga gcaatcacaa caatggcacc catcgctttt
    34981 gtgacgatga cagagcatat cggccatctt atggtattga ataaactaac taaacgtaat
    35041 ttctttgaag aaccgggtct tcataagacg ttgacaggtg atggattagc acaaatcgtg
    35101 gcagcctttg tcggaggtcc tcctgtaaca agttacggtg aaaacattgg tgttctggct
    35161 atcacacgtg tccatagcgt gtttgtgatc ggtggagctg cagtatttgc agtcatttta
    35221 ggattcgtag gaaaattaag cgctttgatt ttaagtatcc ctggaccagt catctcagga
    35281 atcagtttta tcttatttgg agtaattgcc gcaagtggaa tgaagatttt aatcgagaac
    35341 aaaatcgatt ttgataaaaa gaaaaatctg ttgattgctt cagttatctt agtagtagga
    35401 attggcggac tcgtctttga agtaggcaca tttacattgt cggcaatggc attagcaact
    35461 gtacttggca tcattttaaa tcttatctta ccagaaactt cacgaagcga agaaaactaa
    35521 ggaggaaaca ttatgatcat caaatcagaa cgtatcagct taaagcattt attgaccgtt
    35581 gaggcactta ctgatcaaga agttatgggc ttgatccgca gagggcagga attcaagcaa
    35641 ggtgctaatt ggactccgca aaagcgccag tactttgcta caaacctctt tttcgaaaat
    35701 agtactcgca cacataaaag cttcgatgta gcggagaaaa aactagggct agaagtgatt
    35761 gaatttgaag caagcacaag ctctgtccaa aaaggggaaa cactatacga tacagtactg
    35821 acgatgtcag cattaggcgt agacgtagcg gttatccgtc atggtgatga gaactattat
    35881 gacgaactga tacaaagtaa aacaatccag tgttcgatta tcaatggagg agacggaagc
    35941 ggacagcatc caacccaatg cttgcttgat ttgatgacca tttatgaaga atttggtcat
    36001 tttgaaggtc tgaaagttgc catcgtaggt gatatcactc actctcgcgt agctaaatcc
    36061 aatatgcaga tgttaaaacg tctaggcgca caagtattct tctcaggtcc tagagaatgg
    36121 tacgacgatg aattcgaagt gtacggacat tatatgccat tggatgaact actagatcaa
    36181 gttgatgtca tgatgatgct tcgggtgcaa cacgaacgcc atgatggaaa agaaagtttc
    36241 tcaaaagaag gctaccatca ggaacatggg ttaacagttg agcgtgcgaa gaagttgcaa
    36301 aaacatgcaa tcattatgca cccagcacca gttaaccgtg atgtagaatt agctgattca
    36361 ttagtggaag gattacagtc gcggatcgtt cagcaaatga gcaatggagt attcgtcaga
    36421 atggcaatct tagaagcagt gctaaatgga aaagcgtaat tataggagga tgaaaaaatg
    36481 aaaacactca ttaaaaacgg tcagatcaat acacgaaaaa atatgacgac accagcagaa
    36541 atttggatag aagatgggcg aatcaaagca atcggtacag gattctcaga agccgaattt
    36601 gatgaagtgt ttgatgcaaa gggacaattg atcacacctg gtttagtgga cgtccatgtt
    36661 catttgagag agccaggatt tacatacaag gagacgatcg aagctgggac tcgctcagct
    36721 gctcgtggcg gtttcactac tgtatgcgcg atgccgaatt taaatcctgt acctgatacg
    36781 gcagaaaagc tgagacaagt atatgacatc attagaaaag atgcagttgt gaaggtattg
    36841 cagtatgcgc caatcacaga aaatctgcga agtgagaaat tagttgatca agaagcactg
    36901 attgaagaag gtgcttttgc tttcacaaat gacggtgtag gtgtccaaac agctgggacg
    36961 atgtatctgg ctatgaaaga agctgcaaaa aacaataaag ccctagtagc ccatacagaa
    37021 gatgaatctt tattatttgg cggcgttatg cacgctggca agaaggcaga agaacttggt
    37081 cttcccggga tcttaagcgt aaccgagtct tctcaaattg ctagagatct gttgctagct
    37141 gaagcaactg gtgttcatta ccatgtttgc catgtttcta ctaaagaaag tgtccgtgta
    37201 atcagagacg caaaaaaagc aggaatccac gtaacagcag aagtttcacc ccatcatctt
    37261 attttgatcg acgaagatat tcccgaagat ttcggctttt ggaaaatgaa cccgccgcta
    37321 agaggacggg aggacagaga agctttgatc gaaggcttgc ttgacggtac gatcgattgc
    37381 atcgctacag accatgcccc ccatggatta gaagaaaaaa gccaaagttt catgaaatca
    37441 ccatttggta tcgtaggaag cgaaacagcg tttcaactga tctatactca ttttgtagaa
    37501 actggtcgat tcacgttgga acaagtgatc aattggttgg cagttaaacc ggctgagatt
    37561 tttggtttga atgcaggaac gctaacggtt ggtgcccccg ctgatgtagc agtttttgat
    37621 attacacaaa cttgcacgat cgataaagaa gactttttat caaaagggga gaatacacct
    37681 tttatcggct ggaaagtaaa aggcgaaaca caaatgactt ttgtcaacgg aaaacttgtg
    37741 tggcagaagg gagaataaaa gtatggaacg tttgcttata ttagaagatg gaacagtttt
    37801 cgaaggaaag gcttttggtg cagatgctag tgtcttcgga gaaatcgttt ttacaacaag
    37861 tatgactggt tatcaagaaa ctatcactga tcaaagtttt aacggtcaaa tcattacatt
    37921 tacctatcct atggtgggta attatggggt aaatagagat gattatgaat cgatttctcc
    37981 aacatgcaaa ggagtagtag taaaagaaca tgcacgtgta gcttcaaact ggcgaaacca
    38041 aatgacgcta gattcatttt tgaaacaaaa aggaattcct ggaatatcag gtatcgatac
    38101 gcgcgccctt actcgcaaaa tccgtgaagt cggaacgatg aaagctagta tagttgatgc
    38161 tggagattcc ttcgaacatg catttgatca gctgaaagca accgttctag caaccaatca
    38221 agtgcagcaa gtctcaacta acaaacctta tccaagtcca ggaacaggtc ggaaagtcat
    38281 tgtcattgac ttcggcttga aacatagtat tttacgagaa ttgagtaaaa gaaactgtaa
    38341 cctcacagta cttccctata acacagatgc cgagacgatt ttagcgctat caccagacgg
    38401 agtcatgctg acgaatggac ctggtgatcc aaaagatgtg ccagaagcaa tcgaaatgat
    38461 caaaaaaatc caaggaaaag taccgatttt tggtatttgt ctcggtcacc agttatttgc
    38521 tttagccaac ggtgcagata cctataaaat gaaattcgga catcgaggat taaatcaccc
    38581 ggtacgagaa gtagcgaccg gaagaattga ttttacttct caaaatcatg gctatgcagt
    38641 cgatgaagcc acgattgatc cagaaaaact catcgtaact catgtagaag taaatgacgg
    38701 aacagtagaa ggagtcaaac acagagatta tcccgctttc agtgtgcagt ttcatccaga
    38761 tgctgcccct ggtccgcatg atgcactaca tttatttgat gaatttatgg aaatgatgga
    38821 cgcaggaaag gagcagtaaa cagatgccga aacgtacaga tatcaagaaa attatggtga
    38881 ttggatctgg tccgatcatc attggtcaag ccgcagaatt tgactacgca ggaacacaag
    38941 cctgccttgc cttaaaagaa gaaggatatg aagtggtttt agtaaattct aatcctgcga
    39001 cgatcatgac ggataaagaa gtggcagatc aagtatatat cgaaccgatc acacttgaat
    39061 ttgtatcaag gattcttcga aaagagcgtc cagatgctct ccttccaact ttaggcggac
    39121 agacaggcct caatatggca atggagctgg cagcatcagg tattttagaa gaattgaaca
    39181 tagaacttct aggaacaaag ctttctgcga tcgatcaagc agaagatcgt gatctattca
    39241 aacagttgat gaaagaattg aatcagccaa tccccgagtc tgaaatcgtc aatacagtag
    39301 aagaagctgt agattttgca aataaaatcg gctatcctgt catcgttcgt ccagccttta
    39361 ctttaggcgg aactggtgga ggtatgtgtg aaaatgaaga agaattacgt atcgtatcag
    39421 aaaatggatt aagtctttcc cccgctaccc aatgtttgat tgaacgaagt attgcaggtt
    39481 ttaaagaaat tgaatatgaa gtgatgagag attcggctga taatgcaatc gttgtctgca
    39541 atatggaaaa ttttgacccg gtgggtatcc atacaggtga ttctatcgta tttgccccta
    39601 gtcaaacatt gtctgaccat gaatatcaaa tgcttcgtga tgcttctctt tcgattattc
    39661 gagcacttaa gatcgaggga ggctgtaatg tccaactcgc tcttgatcca catagtttta
    39721 attattatgt gattgaagtc aatccgcgtg tttctcgttc atctgcgtta gcaagtaagg
    39781 cgactggcta tccgatcgct aagctagctg ctaaaatagc gcttggatta acattagacg
    39841 aaatgaaaaa tcctgttaca ggaacgactt ttgcagaatt tgaacctgca ttagattatg
    39901 ttgtagctaa aattcctcgc tggccgtttg ataagtttga aaacggagaa cggcttttag
    39961 gcacgcagat gaaagcaact ggtgaagtga tggctatcgg tcggaatatc gaagagtctt
    40021 tattgaaagc tgtgcgttct ttagaaatcg gtacacacca tatcgaactt cctgagttgg
    40081 cagacatcac ggacgatgac ctaatccaaa aagtggttcg agcacaagat gatcgcctgt
    40141 tttatgtagc tgaagctatt agaagagggt acaccataga agaattagcg gatttgacaa
    40201 aaatcgattt attcttttta gataaactgt tgcatatcgt agaaatcgaa cagcagttga
    40261 aacagcagcc gttggatgac aagatattga aaacagcgaa acaaaacggt tttacagaca
    40321 aaaaagtggc ggagctgtgg gaaataaacg aagaagccgt tcgccagatg agaaaagaaa
    40381 atgatttgat tcctgtatat aagatggttg acacttgtgc agcggaattc gaatcaaaaa
    40441 caccttattt ttatagttcc tatgaatatg aaaatgaaag ttcacgatca gaaaagccct
    40501 caattctagt actgggatct ggtcctatcc gtatcggaca aggggttgaa tttgactatg
    40561 cgactgtgca ttctgtcaaa gcaatccagc aggcaggata cgaagcaatc atcatgaaca
    40621 gcaatcctga aacagtttcg actgactttt ctatttcgga taaattatat tttgagccat
    40681 tgaccttcga agatgtgatg aatgtgatcg agctagaaca gccgatagga gtaatcgttc
    40741 aatttggtgg tcagacagca atcaacttag ccgaaccttt gactgctgct ggtgtaaaaa
    40801 tcttagggac aacgatcgaa gacttggatc gtgcggaaaa tcgtgattta ttcgagcaag
    40861 cgctgaaaga gttggatatt cctcaacctt taggggatac tgctacaagt aaagaagaag
    40921 ccgtggttat cgcttcgaga attggttatc cagttcttgt tcgtccaagc tatgtattag
    40981 gcggacgtgc gatggaaatc gttgaaaacc agcgagattt agaagattac atggaacatg
    41041 ctgtgaaagc atctccagaa catccagtac tagtggaccg ttacttgctt ggtggtgaat
    41101 gcgaagtgga tgcgatttgt gacggtgaaa cagtactcat cccagggatc atggagcata
    41161 ttgagcgtgc tggtgtccat tcaggggatt caatggctgt gtatccacct caaacattat
    41221 ctgaatcgat caagaaaacg atcgaagatt acacgatccg cttagcatta ggtttgaatt
    41281 gtgtcgggat gatgaatatc cagtttgtca tccatgaaaa tgaggtttat gtgatcgaag
    41341 tcaatcctcg agcaagtcgg actgttccat tcttaagtaa ggtgacagga attccaatgg
    41401 cacaaattgc gacgaaagct attttgggag agaaactaaa gacacttggt tatgaaaacg
    41461 gactttatcc agaaacaaaa gaagttcatg ttaaagcacc tgtcttttct tttacgaaat
    41521 tgcagaaagt tgatacttac ttagggcctg aaatgaaatc gactggagaa gtaatgggat
    41581 ctgacagaca attagacaaa gctttgtaca aggcattcga agcttctgga ttacacttgc
    41641 ctgaatatgg agccgtatta tttacgattg cggacgaaac aaaagaagaa gcactagaat
    41701 tagctaaacg ttttacagaa attggctata gtctgatagg aacgaaacat acagctgctt
    41761 atttcgaaaa aaatggtctg gtcgttgaaa cagtagcgaa aatttctgag gaagcagcag
    41821 aaaaaaatgt agtcgactta ataagagatg gtaaaacaca agtggttgtc aatacgattg
    41881 ataaagatcg gggaaatgct tctaaagacg gtttcatcat ccgaagagaa gcagttgaac
    41941 atggtattcc attgtttact tctttagaca cagcagatgc gatcgttcga gtgatggaat
    42001 cccgtgcttt ttcaactaac cctatttaac ggcgagcaca tgttaagaca aaaagacgaa
    42061 aaaccgttgc aggaggggac agatgaaaca agaaatgatg gaaatcgttc agcaaaaaca
    42121 gctggcacca aagatttttg aaatgacatt gaaaggtgat ctagtaaaag aaatgcagca
    42181 accaggacag ttcttgcata ttctcgtgcc gcgtgcagac ttgctattac gccgaccgat
    42241 tagcttgaat catatcgacc acgaaacaaa tacttgccgt atcatttatc gggtggaagg
    42301 tgaggggact agagtcttct ctactctttc tgccggagac catttagatg tcatgggtcc
    42361 tttaggcaat ggttttgatt tgtccaatct tcatacagga gacgaagtat ttataatcgg
    42421 tggaggaatc ggtgtccctc ctctatatga gttatcaaaa cagttagtcg aaaaaggaat
    42481 taagccaacg cattttttag gttttgccac acatgaagtc gtttattatg aaaaagaatt
    42541 taatcgtcta ggagaaacaa gaatcgctac tgacgatgga acatacggta tgcacggaaa
    42601 tgtcggtaat ttattgttag gaacgaagac ggaacctaca gctgtttttg catgtggttc
    42661 aaacgggtta ttgaagacag tggaacagct gttttcttcg catcaaaatg tccagttatc
    42721 attagaatcc cgcatggcat gtgggatagg ggcttgctat gcttgcgttt gtcatacgcc
    42781 ggaagatgaa acaggaacaa aaagtgtgaa agtttgtgac gaaggaccga tttttaaaat
    42841 aggagaggta attatatgaa tgagaatcct ttagcggtca aacttcctgg cctagaatta
    42901 aaaaatccta tcatgccggc aagcggctgt ttcggttttg gcaaagaata cggaaaatac
    42961 tatgatttga atcagcttgg atcgatcatg gtcaaggcaa cgactccaaa tgcccgcttt
    43021 ggcaatccca cgccaagagt ggcggaaaca ccatctggca tgttgaatgc aattggtttg
    43081 caaaatcctg gtctagacgt tgttatgcat aaacttcttc cagaattaga ggagtacaat
    43141 gagttaccca tcatcgcaaa tgttgctggt gcttgtgaag aagactatgt agaagtttgt
    43201 agcaaaattg gcgaagcacc aaatgtgaaa gcaatcgagc tgaacatttc ttgcccaaat
    43261 gtcaaacatg gcggaattgc ttttggaaca gattctgatg ttgcttttca attaacacaa
    43321 gcagtaaaga aagtttcttc tgttcctgtc tatgtcaaat tgtccccaaa tgtaacagac
    43381 atcgttccaa tcgctcaggc aatcgaagct ggaggggcag atgggtttac gatgatcaat
    43441 actcttcttg gtatgcggat tgaccttaag acaagaaaac cgattttagc caatcaaaca
    43501 ggtggactct ccggaccagc aatcaagcct gtcgctattc gtttgattca ccaagtagca
    43561 agtatatcca gccttccaat cattgggatg ggcggcgtgc agacagtgga tgatgttctt
    43621 gagatgttta tggctggagc aagtgctgta gctataggga ccgcgaattt caccgatccg
    43681 tatatctgtc ctaaactaat taaagagctc cctgtgcgaa tgagcgaact aggcatcgaa
    43741 tcgctggaaa gattaatcaa agaagtgaga gaggaacgtg aacgatgaat caactagacc
    43801 aacgaccaat tattgcatta gatttttcaa ctaggcaaga ggtagaagac tttcttcgat
    43861 ttttcccgaa agaagaaaaa ttgtttgtga aaatcggaat ggaattgttc taccaagaag
    43921 gacctgaaat cgtacgttat ctaaaagagg ctggtcataa tgtattttta gatctgaaac
    43981 ttcacgatat tccgaatacg gtcgaaaaag caatgcgggg attggcaaag cttggtgttg
    44041 atctcacctg tgttcatgcc gctggcggta ttcgtatgat ggaggcggca atgcgaggat
    44101 tagaagaggg gactccagaa ggtggaaaac gtccgctact tctagctatc actcagctta
    44161 cttctacaag tgaagaagaa atgcatgccg atcaactaat cgaagtttct ttagaaaaaa
    44221 gtgtgatcca ttatgctagc tgtgccaaaa aagcaggatt agatggtgtg gtttcttctg
    44281 cttgggaagt agaagcgatc aaagaaatgg caggagacga atttgtttgc ttaactccgg
    44341 gtataagacc agaaggaaca gtcgcaggag atcagacaag agtagtcaca ccttctcagg
    44401 caaaaaagat cggctcaact tttatcgtta taggtcgacc aatcactcag tcaactaatc
    44461 cttataaagc atatcaaacg attcaaacag aatggagcta acctaaaatg aatgttgaac
    44521 aaagtatcgc aaaagactta ttagaaattg aagcagtatt tttaaatcca agtaatcctt
    44581 ttacatgggc aagtggaatc aaaagcccaa tttactgtga taaccgaatc acgatgagct
    44641 atccaaaagt ccgaaaagag atcgcaaaag gattagcaag caaaatcaaa gaagcttacc
    44701 ctgaagtaca agtgattgct ggaactgcaa cagcaggaat ccctcatgcc gcatgggtag
    44761 cggaaatact ggatttgcca atggtctata ttcgaagtaa ggcgaaagat catgggaaag
    44821 gtaaccaaat cgaaggacgt atatttgaag gtcagaagat ggttgtgatc gaggatttga
    44881 tttctacagg cggaagtgtc ttggaagcag ctgaagcagc aaaaagagaa ggtgcggata
    44941 tccttggtgt tgcggctatc tttacgtatg agttgccaaa aggaaaagca aatttcgaaa
    45001 aagccgaaat ccctcttatg acgttgacca attattctgt attgattgaa gcagctttag
    45061 aagatcgtta tatcgatgag caagaattaa ctctgctaaa agaatggaaa aaagatcctg
    45121 aaaattggca gatttaagcg cgcttgatga agatagagaa atccgatttg tgtctccaga
    45181 atcatcaatt ggaatagcaa aaatccaaaa actgattgcg agaagcatct tttcatcagg
    45241 tatgaaaaga aacttctatc gtgatcagtt tttgattttt ttaaaattca tttttctaag
    45301 actggttagc agaaagaaga gaataatctc ttctcttttt tctattatat atagattttc
    45361 tctatttttt ggtaagggag ctgtttcaaa aatggtaaag agcggaaatg ttgcgaatat
    45421 cagttattta atgatatgat attcggaaaa agaaatctca aagggaggta acttttatgg
    45481 ggttgaatga atttatttta ggattgaaca atttagagac gatcacaagg gcacctggat
    45541 tttttaaata tacagagcat acagttgcag cacattcgta tcgtgtagca tctattgcgc
    45601 aagttctagg agacattgaa gaagcgaatg gacaaccgat cgattggaaa ctgctctatg
    45661 aaaaatcatt gaatcatgac tacacagagc gttttattgg tgatatcaaa acgccggtga
    45721 aatacgcaag caaagagtta agagggatgc tgcaaacagt tgaagaaaag atgacggacg
    45781 aattcatcaa ccaagagatt ccagctgagt tccaagaagt ttatcgccgt cggttgttcg
    45841 aaggaaaaga tgaaaccgta gaaggagaac tattagccat ttcagataag gttgaccttc
    45901 tttatgaatc ttttgatgaa atcatcaaaa ataacccgga aaaagtttat aaggagatgt
    45961 tcattgaggc agtgactacg attaaaagct ttgaacatcg gccttctgtt cagtatttct
    46021 ttaaagagat atttccggaa ctattgaacc aggaatttta tggaaaagag gcgtttttag
    46081 aaaccgtctc aacgattgta aagaaagaag aaaaataaaa atgatatgag atgcaggggg
    46141 aagtatagtt gaaaaagaat atggatgtcg aatggggcta ttctggagac aaaggtccgg
    46201 aaaattgggc gagtttatgt gattggtttg cgagaggtgc ggaatttcca ttgcagtcgc
    46261 ctattcattt gaccaagggg gaagaaacca aaatagaagg agacacgctt tcctttcatt
    46321 atcaaaagca acggtttact gacaaggaat tcaaaaacac gattcattta gtaccttttg
    46381 accaactcag ttatgtagag ttcaagaaag aacgctatta tttgacagat attcatttcc
    46441 acttgccttc tgaacatatc atcaacggaa aacaggaaga tattgaattt cattttgttc
    46501 atatgaacag caaaaaagag aatctagttg tgggtgtcat gtaccagcta atggtacagg
    46561 aaggttggct gtataatcaa gaaaatgggg aaagttggaa cttagaaaaa catgagcatt
    46621 gggtaaatcc ggatgtgttc tttccggagc aaaaaagcca tttccattat attggctcat
    46681 tgacaactcc gcctaccagc gggccaatcc aatggtttgt aatggatcat gtaggaaaac
    46741 tgaaccaaga gtttcttctc cccttcgatg gtcagtatgc tcgaccgaac aaccgaccaa
    46801 tccagccctt gaatggaaga gaaatttatt atttcgagga gtttgagcaa tgaatattca
    46861 gcagatgaaa tacgtggtcg ctgtagcaaa caatggtagt ttcagagaag cggcaaagaa
    46921 aatgtttgtc tcccagccaa gtctttcagc tggtatcaaa gaattagaaa ccgagttagg
    46981 gatcacgctg tttactcgaa cgaatcgagg ggcatattta acagaagaag ggcaagactt
    47041 cttaaatcgt gccgagaaaa tattggtcca gcttgataca ttagaaactc actatctaac
    47101 gagtgaaaag agagacaatt tttcgatcgc tgcccagcat tatgatttct taggtccttt
    47161 gaccgcacag ctactcgagg aattcaaaga cgagatcaaa caatttcgga tcatggagac
    47221 gacaacagcg agagtgatcg aagaagtcaa agagcagcac agtgagatcg gcattattta
    47281 tatgaatgag cataatcaag ctggtatcag aagatattta caacaaggag agctaagtgg
    47341 ccgcagcatt ggaaaattca aaacgcatat ttttttaagg caaggacatc cattagctga
    47401 acacgcagag atcaccaaag aagaattgtt atcttatcct caagttcgtt ttacacagga
    47461 tggaaataat ttcccgtatt tttatgaaga ccttatcgag atccctgatc aagaaacagt
    47521 gatttatacg agtgatcgag gtactttgat gaatatcgtt cttgaaactg atgcttacgc
    47581 ttcaggatct ggtattgtga ttggagggat tcgcgagcac ttgcggttaa tccctttggc
    47641 agaaggcgaa ctaaatgaat tttatatcat ttattcggcg aaacgtccgc tcagtgaaat
    47701 cgctcaaaga tttatcaaag agcttactcg gttactggac cagcagagct aagaaaagca
    47761 gatgagaacg ctgtttattg aacaccgttg atcaaaaaag aggggttgtg acagaatttt
    47821 gtcacaactc ctctttttgt agcaaacaat ttataactaa ttgctgttgt gggaaagtac
    47881 gcaaatatga ttctagaaac actcattgat tacctgtttt gtttcatagc tgtaattttt
    47941 tcttcttctg gtgtcacgat aatcgtcttt tggttttcat aaataacata accaggcttg
    48001 gcgccatttg gttttcggat atgctttacc tgtacaagat caaccggcac ttgggcagag
    48061 tatcggtatt ttgaaaaata ggcagctaac tcggcagctt ctgtaattgt ttcatctgag
    48121 ggcttgtcac ttttaatgat gacgtgcgaa ccgggtatat tttttgcatg aagccaatag
    48181 tcggtttttt tagctgtctt cattgtcagc tggtcatttt gaagattgtt tttaccaaca
    48241 agaatgagcg ttccgtcaga agaaaagtat tgatcaggct gagatggttt cttacgtttt
    48301 tgttttttta agttcttctt tttcaggtat ccttctgaag tcaattcttc tttaattgct
    48361 tcgatatcca ttgggccggc aatttccagt tgagataaga cagattcaag atattggatt
    48421 tcatctttgg cttcttggat ttgttcgcca atcagcttaa cagcgttctt cagcttttgg
    48481 tatcgatgga aatatttctg tgcattttga tttggtgtca atgccggatc tagtgctatt
    48541 ttgattggcc gatcttcttc ataataatta ggtaaaacaa cttcttttgc gccgcgtggt
    48601 acttgtgtca gaaaagtagt aagtagttcg ccatttctac ggtaattctc agcgttttcg
    48661 gattctttga gtgtttgttc acgttttttc aatttatttt tatttcgttt tagttcattt
    48721 tcgatttttc gaatcagttc tccgccttgt tgttttgccc gatctttctc tgctttttcg
    48781 tgataatagc tatctaataa ttgactgagt gttggataag ctgttacagc agatgcctgt
    48841 tcggacaata cctgataagc aatcggtgtg aaaaaatctt tattttccgt ttcgtaaaaa
    48901 gtaggaatag gctgatggct gatggctgag aaaaattcgt cccatacaac catcttttca
    48961 ttaggacgtt ccagtaatct tttggtcagc tcttgagcga tatcgaaacc gattccctga
    49021 aactgacgtt ggattgcttt tggatctagt tcagtttggg aaagccgctg aaagattttt
    49081 tctttctcgg agctaaatgg gttcaagacc tcttgttttg gcggagcaac atattctaca
    49141 ccaggaagca aagaacgata agtgttttga gagcttccaa tgtgtttgat cgcatccagt
    49201 atttttccag tttctcgatt aactaagacg atcgtactat gacgtcccat taattcaacg
    49261 atcaatatga tattttgtag atcacccaac tcgtttcgtt tagcaaagtg gaaatggatc
    49321 actcgatcat tttcgatttg ttcgatgtct tctaaaatag cgccatccaa atattttcga
    49381 agcatcatca cgaagttagg cggattatca ggattctgat aatcgatttg ggtaatctgt
    49441 acacgagcat aacttgggtg cgcagataat aataatcgat ggtttttgcc gcgtgaacga
    49501 atcaccagca cgatttcatt ttcataaggc tgatggattt tcgagatccg gccagatagt
    49561 aacgtttcac gcaattcgtt gaccatcgcg tgggtaaata caccatcaaa ggacatggtt
    49621 cattcctcat ttcttttcat aagtatattc attataacga aaaaaagaag aaaaaccaat
    49681 ttggccgaat agtaaagcaa aagtctagaa aaatcttgga ctaattacat aaaaaacagt
    49741 gtttctatcg ttgaaatcca aatttttagg tataataaaa aataggagaa aaagtagaaa
    49801 agaaggtgtt tgttatttgg gaagtagtta aatcgagagt acaagaaatg aatgaacatt
    49861 tctcagtgtc attcgcagaa aatccaagta agtattttcg acgtcttgtt tttctgctca
    49921 tcttatcatt gctgttaaca gttgcaatgt ttttattgcc ggaaaaaatc cgagaagagc
    49981 aagtaaatag tctcgtttat tcgtccatac caaccagcaa attgaaacct tttgattaca
    50041 gcaaagctga tcagaaaatc aaacaaagcc gggcgatatc ggtgatgttc agcaaaccga
    50101 acgggaaaac ctatcaggac atgcttgatg tttttaatga tcctgagctt atggaagaat
    50161 tgaatcgacc gatcttttat tatccgatcg tttatgatgt acatgaactg gaacaaaaat
    50221 ataacatacg aacggatgaa gtgacattta ttttctttga tggaggaaaa gaagcaaatc
    50281 ggataactgc gggtaaggga ggaataactg attttgataa ggagttgatc cctgaactga
    50341 atcgacttcc gttagccaat atcaaacaat tagaagaaga attggataga acgggagtaa
    50401 cttcccaaaa ttccgagatt gctaattgag acattggcgc gatgaaacgg tgggtctata
    50461 tagcgctatt tttaattatc agaacaagtg atgtttgatc ttcaaaataa caaataaaaa
    50521 attaaaaaat atagttgacg aaattcaaaa attcgattag acttaaatca attcttaagg
    50581 aaagggtgac ctgtaatgaa cttatcaaaa cgtatttcag cagaaaacaa aaaaaattgg
    50641 cgcagctggc gtagataagt tgtattaatg gtgagacttt cccatagata caacttttta
    50701 gagtgcattt ctaaagcttg tatctatgct agacatgaac attgactgcc gcatagatta
    50761 gaatctatgc ggttttttgt gtaaagacaa tgaccgcttt ggccattgtc tttttttgta
    50821 gactggatac ttgcctgatt ataataaata aaaagattga ctttgcagga ggaaaaaaat
    50881 gaaaaacaaa cgattaataa cagttgtagt aattatttta gtttatttag caggtgcatt
    50941 tatttatgga aaagcctcag gcgaaaaaga aagtacttcg acgaaccaga caaaaaaaac
    51001 aacagttgtt ggggtattgc aatacgtgag ccatccggca ttagatgaga tttatcgagg
    51061 aatcaaagat ggtctgaaag aagaaggatt aaaagaagga gaaaatcttg agatcaaatt
    51121 tcaaaatgga caagccgatc agagcaagct agccacaatg agtcaacagc tagtacaatc
    51181 tgatccagat gccttgattg gaatcgcaac acctgccgct caatcattag caaacacgac
    51241 ttcatcgatc ccagttgtgt taggtgctgt aacggatcct gtgggggctg gattagtcgc
    51301 ttctttagag aaacctggtg gtaatgtaac aggtgtttct gatcagccgc ctgttacttc
    51361 acaaatcaag cttgcttctg atttgttgcc aaatgcaaaa aaagtcggaa tcttgtatgc
    51421 ttcttcagag gataattcaa aataccaagt agcacaagca gaagaagctg caaaaaaaga
    51481 agggttagaa tcagtaaaat atgcggtgcc ttccactaat gagattgcac aaacggttca
    51541 agtgatgagc cgacaagtcg attttatcta tgttccatta gataatacca ttgccaatgc
    51601 gatgcaaaca gtggtaaaag aagcaaacaa agcaaatatc cccgtcattc catctgtgga
    51661 tacaatggtg gaacaaggtg gactagcaac aatcgggatc aatcaatatc aattagggct
    51721 tcaaagcggt aagatggcgg cagaacttgc gagtggaaaa gaaaaaccgg aaacgacgcc
    51781 tgtttataca tttgatcaag gggatacagt gatcaatcaa tctcaagctg atcgcttagg
    51841 aatcattgtt ccccaatcga tgaaggaaaa agccaaaatc attacagatg actcgcagca
    51901 agaaacatct aaggaggatg acaaatgatc gtatcagcta tttcacaagg catgctttgg
    51961 gcaatattag gattagggat tttcatgact tatcggatct tagactttcc tgatatgacg
    52021 acagaagggt ccttcccatt aggtgctgct gtctgtgtga cagctattac tcatggggta
    52081 gcgccgattg tagctacatt attaggcgta ggtgctggca tgatagcagg actcgttacg
    52141 gggttacttt atacaaaagg aaagataccg gttattttag cagggatttt agtgatgtca
    52201 ggattgaact cagttatttt gtttgtgatg cgaacaccaa atctttcttt attgaatcat
    52261 ccgattctac aagacagctt ccatcgcttt gggttacctg attactttga cacagtcctt
    52321 cttggcttca tctctttggg catcttgatt gctgtactgc tattcttctt caatacagat
    52381 ttgggtcaag gctatattgc gacaggagac aacgaaacga tggctcggtc gttagggata
    52441 aaaaccgatc gtatgaaaat tttaggttta actttgtcga acggtgtcat tgcattatcc
    52501 ggtgcgctga ttgcccagaa cgacggatat gcagatgtaa ataaagggat tggcgtaatc
    52561 gtcatcggtc ttgcttccat catcatcggt gaagtgatct tccaagaatt gacgctagca
    52621 gaaagattga ttgctattgt tgtcggcagt atcatttatc agctattgat tttagtagta
    52681 atcaaactag ggtttgacac gacttatctg aagttgttct ccgctatcat cttagctatt
    52741 tgtctgatga tcccgcaatt gaagaaagca ctgaacttaa aaacgggttt tgagaaggag
    52801 gcatagtaat gactgatata ttgacaatca aaaatggcag aaaagtcgta aacaatggaa
    52861 tgaatgaaga gaaagtacta ttggatgaaa ttaaccttac aatcagagaa ggggacttca
    52921 tcactgtcct tggcgggaat ggtgctggaa aaagcacact gttcaatacg atcgctggta
    52981 cgcttcaact cagtcaagga tcgatctctt ttaaagatca gctgatcaca aaagattcag
    53041 aagaaaaaag agcacgattc ctttctcgag ttttccaaga ccccaagatg ggaacggctc
    53101 ctcggatgac agtagcagaa aatttgctac ttgcacaaaa aagaggtcaa aaacggaaat
    53161 tccgtttacg taacttgaaa aatcaaaaag aagatttttt cagactatgt caagaagtcg
    53221 gtaatggcct agagcaacac ttggatactc cagcgggtga attatctggt ggtcaacgcc
    53281 aagcattaag cttgctgatg gcgacgatca cagctcctga gttactatta ttagatgaac
    53341 atactgcagc tttagatcct aaaacagcta aagcattaat gcgattaaca aatcagcgga
    53401 tcaatgaaca aaaattgact tgtctcatga tcacgcatcg aatggaagac gccttgaaat
    53461 acggaaatcg tctgatcgta ctccaaaaag ggaaaatcgt tcaagatatt tctgaaaaag
    53521 aaaagcaaca tttgacaatg ggcgaccttt tgcagttctt tgaagaagta gtggattgat
    53581 ttcgacagaa aaattccttt cagtagaagg acttgaaaac tcattgacat cttcctttga
    53641 ttcatcctat acttaaaaca ataaacaatt aaacatattt gggtgttttg taggatcacg
    53701 tagtcaatga ttttaacaat taaatagaat attctttgct ctgacaagta aacgatgaga
    53761 aaagaatttg ctactttgcc acctagacgt ttcgtacttt ttcgtatcaa acgctaagtg
    53821 gcttttttta ttgaagtaag aacaaaggag gaataagatg atttctggac aaaccaaatt
    53881 agctgggctc tttgctgatc cggcttctca cagtttatcc ccattgatgc acaatactgc
    53941 ttttgaagca aacaacatcg atgcggtgta tttagctttc caagtaaatc caatgaattt
    54001 gaagcaagca gtcgaaagta ttcgaacttt tgatatgtta ggtgtaaatc tttcgatgcc
    54061 gaataaaaca gcggttattc cttatctgga tgaggtaagc caggaagcgc aattgatcgg
    54121 agcagtgaat acgatcgtgc acgaaaaggg tcgtttgatt ggctataata ctgatggtgc
    54181 gggcttcatg caatccgtgc aagaagctgg gatttcgatc aaaaagaaaa aaatgactct
    54241 actgggagct ggaggtgcag caaaggcagt cactgttcaa gcagcgttag acggcgctga
    54301 ggaaatcgta gtttataaac gaaacaacca aacattcgct caagtaaaag aaacattcga
    54361 acaactcagc tatcgaactt cttgtaagat aaaagttcgt gactttgccg atacgcatca
    54421 attaagaaaa gacttgaaag aaagctgtct tctagtcaat gcaacagata tcgggatggg
    54481 aaagaaaatc aatcagtcac cgctcttaga tataagcatg atgcatgaga atctagcggt
    54541 ttttgatttg atttatgcac ctagagaaac acgtttatta aaagaagcaa aaaaagtagg
    54601 agcaaaagga tacaatggac ttggcatgtt gatctaccaa ggagcaatcg cgtttgaatt
    54661 atggacaaag gaaaagatgc cgatccatgt gattgaaaag ctgtttgatt aaaaaaggat
    54721 aatagataaa gatcttttat tctcgcataa acggtctcaa gaaaagtaat cctttatgta
    54781 tcgaagcatt tatttcgtta ttttttatta aattatcaaa aaatgatagg cttttgtcga
    54841 ttggttatcg tatcctttat ttatcagtaa gaaacgagga ggaaccacaa tgagtcggat
    54901 ggatcgctat aaggacatcc ataagaaggc gaaacctctg aagcgggata cggggagtat
    54961 ttttgctaga agagaacgaa aacgaacgcg tatggaaaat gaggaagatg ctacccgtat
    55021 ttatgaaaga gagcagcatt catttgaaca tttgaaccaa tacgagcaac agatatacag
    55081 agatgcacca agagcagaag aacaacaaaa aaataaaaaa tttttcaatc ggcaaaagaa
    55141 gaacaaaccg ttaaaacctc gaagaaagcg atcgtggaaa aaaatcattc tatcgatttt
    55201 gcttgttttg atcctttatt caggtatctc attttttatt ggtaaatatg tagctgaaca
    55261 tgacacaagc ttagaggcag cgacttcaga aacctttcat gggcaaaaag cgacgaatgg
    55321 tgcgcataat attttgatcc ttggaagtga tacacgcgga gaagatgccg ggcgagccga
    55381 tacgatcatg gtcttacagt tggatggtcc ttcccataag ccgaaactga tttcttttat
    55441 gcgtgacagc ttcgttacga tcccaggggt aggacaaaat aagatcaatt cagcttatgc
    55501 atacggagga gccgatctag tgcgtcaaac tttagtagaa aattttggaa tcgattgtca
    55561 atactatgcg aaagtcgatt tcaaatcgtt tgaaaaagtg atcgatgcct tattcatgaa
    55621 cggagtgaag atcgatgcag aaaaagatct gaatctggat ggtgtagata ttaaaaaagg
    55681 gggccagaag atggatggac atgttctttt gcagtacgca cgtttccgaa tggatgaaca
    55741 aggagacttt ggccgtgtca gacgccaaca gcaagtaatg aacgctattt tcagccaatt
    55801 gaagaatcca ttgaatctga tccgtgcacc ttatgcagct ggtaaagctt taggatatac
    55861 atccacagat gtcactgctt ggttcttgac gaaaaacctc ttttcgattg cacgaggagc
    55921 tggtggcatt gatcgaataa gtgtgccagt agacggttcg tggaattatg ggcaaagtga
    55981 ttatgctggc agtattcttg aaattgataa tcaaatgaat caatctgcta ttacagattt
    56041 tttgaataaa tgatcataaa aaagatcaag aagaagctgc gcaaggcttt tgtcatgaat
    56101 caatcaaacg attcactcgt aaaaaacgct ttcttatggt agatttaacc aagagaggag
    56161 ctgtttacat tggatatcta tctgaacttc aaaaatgagt cgcgtgaagc catcgctttt
    56221 tatgaaaaag tttttcagac tgaagcatca ggtatcacca catacggtga aatgcctgga
    56281 atagaacaaa tgaacttgcc agaagaagca aaaaatctaa tcacgaatgc acggctgaac
    56341 atttatgata cattagttat gtttgcagat acgccagaag gaattggtcc tgaattagtt
    56401 gtgggaaata atgtttcttt agtcatccag acaagaaatg aagaagaaac gaagcgccta
    56461 tttgaaagct tagctgaggg tggtaagatt gagatgccac tggatgctgt tccgtgggct
    56521 cagttgtatg ggatggtaac tgatcgattt ggcatcaact ggcagattaa ccaagagtga
    56581 cagctaaaga tgaatatttt ctggaaacat caagcagcaa acggatcatc gtttgtttgc
    56641 ttgatgtttt tgtgtctatt tcgactatac ggtatctttt ttttattgcc agatgcgaaa
    56701 atatgttcca taatagagaa gtccgcaaaa aattaagtaa cggaggtcag aagatgaaga
    56761 cggtgatact agctgaaaaa ccttcacaag caaaagccta tgccgaaacc ttccaacagc
    56821 aaaagcggca tgaagggtat tatgaaatca gcgatccatt gtttgctggt gaagtaacca
    56881 tcacttatgg ttttggacat ttggtagata tggttgctcc aggagcttat gaagagcgtt
    56941 gggcaaaatg gtctttagaa aatttgccta ttttcccaga aaccttccgt tacgaagttc
    57001 caatggacaa acgtgcacaa ttttcgatcg taaaacgaga attgcagtca gccgatacca
    57061 tcattattgc aacagatggt gatcgagaag gtgaagccat cgcgtggtcg attattcgac
    57121 aagcaaacgc attttctaag gaaaagaact atttacgttt atggatcaat tctcttgaaa
    57181 aagaagcaat ctatgaaggt tttaagaatc tccaacctgg aaagaactat tttccaaaat
    57241 ataaagaagc gcaagcaagg cagaacgctg attggctgat tggaatgaat ggaagtccat
    57301 tatatagctt gctacttcaa cgaaaaggga ttgatggcag tttttctctt ggtcgtgtac
    57361 aaacaccgac actctatatg atttatcagc tgcaagaaga aatcaaacac ttcaaaaaag
    57421 aaacgtactt tgaaggagaa ggtttaatta cgacaaacaa agggcagttt tctggaaaaa
    57481 tcgtaccaaa aaaagcattt aaaacccaag aagagttaac gaaggaaata gaaaaacttg
    57541 gagcgcattt aggtaagcaa aacggacgga ttcttgaagt tacgaaaaag gaaaagaggt
    57601 tgcatagtcc acgtttattt tcgctatcta gtttgcagac aaagatgaat cagctaatga
    57661 aagcaagtgc caaagctaca cttgatgctg ctcaaggatt gtacgaaagc aagtttctaa
    57721 gctatccaag aacggattcc ttttacatta cagaaaatga gcatcagtat ctggttaaac
    57781 atctgcaaaa ttataaaaaa tttttaggaa tagaagaagt acagacaact gagatcaaag
    57841 caaagaaacg atatgtcgat gcgaaaaaag ttcaagaaca tcatgcaatt attccgacaa
    57901 agacgattcc gactcctgca cgctttgcga agctttccaa attacagcaa gcaatctatt
    57961 tgcaagtttt gcgcactact gtggctatgt ttgctgcaga ttatatctac gaagagacga
    58021 ccgtaatgac gggcgtacag caattaaagc taaaaagcat cggaaagatc ccattacagc
    58081 aaggttggca ggaaatctta cgcatcaagt caaaaaagaa agaagctcag actcttcctc
    58141 ctatcgtaga aaatgaagaa gcagaagtag aactgaaaag tatggagaaa gaaacacaac
    58201 ctccaaaacc ctataccgaa ggaacgttga tcacagcaat gaaaactgcg ggaaaaacgc
    58261 tggataatga tgaagcgcaa gaaattctca aagaagtaga aggaataggg acagaagcta
    58321 cacgagccaa tatcattgaa aatcttaagc agaagcatta catcgaagtc aataaaaacg
    58381 aaataactgt tacgccaaaa ggaatcactc tttgtaaggc agtagccaat gaaccattac
    58441 tgacgagcgc agaaatgact gcgcgctggg aagggtactt gagaaaaatt ggtaaacaag
    58501 aggggacacc atctgttttt ttagagaata tcaaaaaatt catcctacat ttattggcag
    58561 aagttcctaa tcaaatcgac aaggtagact tttcagatga gattagcggg acacgtcaga
    58621 taaaagcgat ggagaagaaa aacgatcaat taggaagatg tccaaaatgt aaaaagggaa
    58681 tagtcatgct ttatcctaaa atcgctacat gcttgaatcc ggaatgcgac ttcaaattgt
    58741 ggccaacagt agctaaaaag aagctgacta aaacgaatct cagagatttg ctttcaaaag
    58801 gaaagacaag taaagttgtc aaaggtttta ctggtaaaaa agggaaattc gatgcagttt
    58861 tagtattaaa agaagatatg acaatcggtt tttcttttcc atggatcgaa aacagtggaa
    58921 caaaagcagt tgatggaaac aaagaaacat caagaaaaaa taatgaaaac gaagtagagt
    58981 aaatgaaaac aatgaaatag cggaatattt attccgaaaa gtgagaatat aaataccaat
    59041 aaaactaaaa aatatcagtg aatcaagcaa aaacgttggg gtaggctgat tttatataga
    59101 tagcaaggag tttttttatt tgaaaaaagt gcattcattt ttaaattatc ttatgggagc
    59161 aatcacgatt gtggctgtgt accaagcatt tctaaagtat cctgattacg ggacgaagtt
    59221 gttgtcattg acgcacaatc aaacagcata ttcaccaacg ttgattttat cgactttgac
    59281 tgcaggagct aaaagcgcta cttatttttt tcataaaacg acaagcatgc taaagcgaaa
    59341 gaaacagcga aaacaattgt tggtggtggt agcggctatg atcattattt attttctacc
    59401 agttattaca caagtatttc atcaatattt tggttaagaa tacgagagtt tgtgacaaaa
    59461 gtcatgtcac aaactttttt ctttgtcatt ttttcgtaag aaaaagcaaa ataaaagtat
    59521 atacattgat taaaagtata tacttggata taatgtactt actatagaat gagaatggta
    59581 tagagggtgg cgaaatatga aaaaaatgaa aaagtataaa attagaaaat ctggcaatag
    59641 cgatgtaaca actatcccac cagaagtaaa agaattcatg ggtgtgcaga caggtgacgc
    59701 aatcagttat gtttttcaat ctgatggttc tgtacgaatg atcaaagcac aagaagaacc
    59761 tgatattgat tcattagttg attcgatcat gaatcaatat gaagacgcgt taaaagattt
    59821 ggttgattta tgaggtatct ttcgactaaa gaaattatca aaatcaacgc gaaagttatt
    59881 ttacgtcact cgccaggcga aatgatcggt gtaaaagatg caaatgcatt agatatggcc
    59941 gtcaaacagc cttcacaagc agtattcaat caagaactct atcctgaaat ctatgaaaaa
    60001 gcggcgattt tagcaattaa tttagctaaa aaacatcctt ttcataatgg caataaacga
    60061 actgctcttg ttgccatgtt gttgtttctt caattgaata attgtccagt tgctttttct
    60121 cgtcaagaag ctgttgattt tatcataatg attaccacta gttcattgga tttcgattca
    60181 ctaaagtcca agataaccag ttatttacga caaaccgcaa taaaatgaac gaattaaaaa
    60241 ctttatttct caattagatc agcatgtcat taaggaatat gctgattttt ttatgtttaa
    60301 tcatacaaaa tagttttttc ataaaaatat ggaaaatatg gattaatttt gaaataattg
    60361 atttaaaagg aatacaaaag agtgaatagt aggaaatagt ggtttagaac tagtgggctg
    60421 attttttata tattttagtt tacataatat atattataca aagtagattt aaaaaatgat
    60481 aaacctctct cgaacggttt tttatcaaaa atcgtcattt ttccat
//
LOCUS       DACEPW010000002        43699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEPW010000002 DACEPW010000000
VERSION     DACEPW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.002"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..339
                     /gene="tnpB"
                     /locus_tag="IW819_000060"
     CDS             <1..339
                     /gene="tnpB"
                     /locus_tag="IW819_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1243202.1"
                     /translation="IKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKAD
                     WYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTE
                     GLRLHSMGLA"
     gene            complement(494..1222)
                     /locus_tag="IW819_000061"
                     /pseudo
     CDS             complement(494..1222)
                     /locus_tag="IW819_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1344..1928
                     /locus_tag="IW819_000062"
     CDS             1344..1928
                     /locus_tag="IW819_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243203.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFICRDGQEIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIDTSIKELREYVQNPYNFQYLAGRINLV"
     gene            1939..2184
                     /locus_tag="IW819_000063"
     CDS             1939..2184
                     /locus_tag="IW819_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243204.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLVLNKRKKDR"
     gene            2190..2912
                     /locus_tag="IW819_000064"
     CDS             2190..2912
                     /locus_tag="IW819_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243205.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSDNNNKDTKSSNTVVTKN
                     STTSASTTMSSMIENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWVQNIHGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3043..3786
                     /locus_tag="IW819_000065"
     CDS             3043..3786
                     /locus_tag="IW819_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243206.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKNEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWNSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRSKLIKELVQNTWDQAIVELEKENGKILANKYLIVNEIEELIGLF
                     SCKKGEVFLKSLPTYSILDSPYQLIQARR"
     gene            3792..4157
                     /locus_tag="IW819_000066"
     CDS             3792..4157
                     /locus_tag="IW819_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243207.1"
                     /translation="MSSLKDDYIQAKQEYEKLNMLKSEKVELFLTQKLAEEHISEIDA
                     KTLERYDQQFNQIHHNIILLVDQAKRRLEKAEYDLLDQGIVCLDKGIRRYYSAELKKQ
                     NVDRNALKNEILELIMQRI"
     gene            4173..4703
                     /locus_tag="IW819_000067"
     CDS             4173..4703
                     /locus_tag="IW819_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243208.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMMYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NEKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            4832..5092
                     /locus_tag="IW819_000068"
     CDS             4832..5092
                     /locus_tag="IW819_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243209.1"
                     /translation="MAYENLQLVVAGVSGDIYLAKAKDGIMDTNYRRVATDDVLSSAT
                     EWFRKNEESAVHFEGIDGNTHSLFYTKDKEKAKKIIDILKER"
     gene            5292..7220
                     /locus_tag="IW819_000069"
                     /pseudo
     CDS             5292..7220
                     /locus_tag="IW819_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7358..8155
                     /locus_tag="IW819_000070"
     CDS             7358..8155
                     /locus_tag="IW819_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243210.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYGESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSKEEVRELVETSVRKKIETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYNSNRFDRLEKFCRPYTVLKTKKFNKTKRVS
                     IKLPTGQISLGEKDYLIKVGKELIIKVPERLFKENFEIRETLVWKEE"
     gene            8195..8377
                     /locus_tag="IW819_000071"
     CDS             8195..8377
                     /locus_tag="IW819_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243211.1"
                     /translation="MAVNRKLQVLITDDFGGSEWFDVQSIDFEEEVVVIENVYGEEYS
                     YSLEGENVEDLRLIGE"
     gene            8421..8708
                     /locus_tag="IW819_000072"
     CDS             8421..8708
                     /locus_tag="IW819_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243212.1"
                     /translation="MINKKTGKRIFSSKDEVIRDLSENFEPIELFFHQLNLEIEKLLK
                     EPTEGRINKVLKLIACKDSHAYMLYPKWYADKNIAEINDYLASIKERNAIN"
     gene            8745..9032
                     /locus_tag="IW819_000073"
     CDS             8745..9032
                     /locus_tag="IW819_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243213.1"
                     /translation="MAEVKFGSKEWFEELNSMIQFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            9150..9467
                     /locus_tag="IW819_000074"
     CDS             9150..9467
                     /locus_tag="IW819_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243214.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKIYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            9470..9704
                     /locus_tag="IW819_000075"
                     /pseudo
     CDS             9470..9704
                     /locus_tag="IW819_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9871..11259)
                     /gene="ltrA"
                     /locus_tag="IW819_000076"
     CDS             complement(9871..11259)
                     /gene="ltrA"
                     /locus_tag="IW819_000076"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1243215.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(11719..12198)
                     /locus_tag="IW819_000077"
     CDS             complement(11719..12198)
                     /locus_tag="IW819_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243216.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAIKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKLLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            12318..13655
                     /locus_tag="IW819_000078"
     CDS             12318..13655
                     /locus_tag="IW819_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1243217.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            13678..14205
                     /locus_tag="IW819_000079"
     CDS             13678..14205
                     /locus_tag="IW819_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243218.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            14192..14755
                     /locus_tag="IW819_000080"
     CDS             14192..14755
                     /locus_tag="IW819_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243219.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            15177..15914
                     /locus_tag="IW819_000081"
     CDS             15177..15914
                     /locus_tag="IW819_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243220.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDESPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPIEEWNMEQGLD"
     gene            complement(15931..16347)
                     /locus_tag="IW819_000082"
     CDS             complement(15931..16347)
                     /locus_tag="IW819_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243221.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIIV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDEMYSFVK"
     gene            16739..17986
                     /locus_tag="IW819_000083"
     CDS             16739..17986
                     /locus_tag="IW819_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243222.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEAGTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGIANGGNVVDISGSSVQ
                     GLKDEITNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINSGSFETAYNVSQLAVSVR"
     gene            18255..20645
                     /locus_tag="IW819_000084"
     CDS             18255..20645
                     /locus_tag="IW819_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ1243223.1"
                     /translation="MKHEKTMILNSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCLDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTSSNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGEKKVKTTDEAGLATFNIVANKTYSLEETGVPTGYTGAFKQEGITVK
                     DDGQTFEYTAKNTLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQIFEYTVINHRVPETVRTGGIGTSAFLLVFLVAGLGMGLLYYREQRKVK"
     gene            20694..22529
                     /locus_tag="IW819_000085"
     CDS             20694..22529
                     /locus_tag="IW819_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1243224.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSFGLGEQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGKPVSDIDNKGKEIALPNGVQPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPVQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     DYEKYVVKDVLGEELTYDSSEVIEGMTLNTDYSIERDGQTTNYVFTDSGMDKLQKKFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNRVTYLKQDPTTKAITWVEDKSKATEFVSGTDGKFQ
                     VEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSK
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            22598..23461
                     /locus_tag="IW819_000086"
     CDS             22598..23461
                     /locus_tag="IW819_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1243225.1"
                     /translation="MSMKKKNIKLIILRIGMLLCLGVGLYPVITQAYTYYQTQQTLNA
                     YKEKYDEASEKENEEIFKKYSEINRKRKETEQTLVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYNGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFINVAGKKLAYKVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVRIPYKKNIVEKEKKKEDLKVKAQINWYPIIVVGVLSIIALIVIIKKI
                     VAKRRGMKNEE"
     gene            23451..24530
                     /locus_tag="IW819_000087"
     CDS             23451..24530
                     /locus_tag="IW819_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1243226.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSESVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLKVLILLIKEFRKA"
     gene            24869..25669
                     /locus_tag="IW819_000088"
     CDS             24869..25669
                     /locus_tag="IW819_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243227.1"
                     /translation="MVGISKTALYSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWANIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            25757..26563
                     /locus_tag="IW819_000089"
     CDS             25757..26563
                     /locus_tag="IW819_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243228.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDQETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            26794..27663
                     /locus_tag="IW819_000090"
     CDS             26794..27663
                     /locus_tag="IW819_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1243229.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            27683..28030
                     /locus_tag="IW819_000091"
     CDS             27683..28030
                     /locus_tag="IW819_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243230.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            28043..29305
                     /locus_tag="IW819_000092"
     CDS             28043..29305
                     /locus_tag="IW819_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243231.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            29385..30761
                     /locus_tag="IW819_000093"
     CDS             29385..30761
                     /locus_tag="IW819_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ1243232.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            30758..31387
                     /locus_tag="IW819_000094"
     CDS             30758..31387
                     /locus_tag="IW819_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243233.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMIYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRIQINKFEKKVRK
                     FNDLKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            31406..31885
                     /locus_tag="IW819_000095"
     CDS             31406..31885
                     /locus_tag="IW819_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243234.1"
                     /translation="MLELFEVNRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRHKKYVLLEYRHKITDEIGVNKTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            32387..32440
                     /locus_tag="IW819_000096"
                     /pseudo
     CDS             32387..32440
                     /locus_tag="IW819_000096"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32444..33043
                     /locus_tag="IW819_000097"
     CDS             32444..33043
                     /locus_tag="IW819_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1243235.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFTLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            33059..33535
                     /locus_tag="IW819_000098"
     CDS             33059..33535
                     /locus_tag="IW819_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243236.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            33552..34454
                     /locus_tag="IW819_000099"
     CDS             33552..34454
                     /locus_tag="IW819_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243237.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            34527..34718
                     /locus_tag="IW819_000100"
     CDS             34527..34718
                     /locus_tag="IW819_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243238.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            34882..35469
                     /locus_tag="IW819_000101"
     CDS             34882..35469
                     /locus_tag="IW819_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243239.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            35469..36368
                     /locus_tag="IW819_000102"
     CDS             35469..36368
                     /locus_tag="IW819_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAQ1243240.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVSDATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRFV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            36368..37282
                     /locus_tag="IW819_000103"
     CDS             36368..37282
                     /locus_tag="IW819_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243241.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLGERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGVRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNAMNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            37334..37549
                     /locus_tag="IW819_000104"
     CDS             37334..37549
                     /locus_tag="IW819_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243242.1"
                     /translation="MKRDVFEFYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            37793..37993
                     /locus_tag="IW819_000105"
     CDS             37793..37993
                     /locus_tag="IW819_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAQ1243243.1"
                     /translation="MGIMFFDRISLKMDLSIAHQEFNLRLKALLNSDDVNFAHDVVGI
                     QNHIDRESKRMGDEFLPRYSSL"
     gene            38172..39215
                     /locus_tag="IW819_000106"
     CDS             38172..39215
                     /locus_tag="IW819_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAQ1243244.1"
                     /translation="MKKILVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPAPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            39387..39614
                     /locus_tag="IW819_000107"
     CDS             39387..39614
                     /locus_tag="IW819_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243245.1"
                     /translation="MNQRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGSK
                     RYEKAEESVDQLEEAVDGLSDILGLIEDITS"
     gene            39617..39874
                     /locus_tag="IW819_000108"
     CDS             39617..39874
                     /locus_tag="IW819_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243246.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSDKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLQSYTTSEKDK"
     gene            40643..41437
                     /locus_tag="IW819_000109"
     CDS             40643..41437
                     /locus_tag="IW819_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243247.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKRRGLIPK
                     KKKGIWREFFKEEDSNRNNGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNIL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVINNFTDLLAFAKKARDLRETQ
                     MYLFRNKNGRNRRTESSDIEDYYDAFTHDLGAFVDEISGVMDKYRV"
     gene            41466..42584
                     /locus_tag="IW819_000110"
     CDS             41466..42584
                     /locus_tag="IW819_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243248.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHYLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSK
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKTLREGEVNTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSEYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSLGKSKDEVKGVVIDDDFEFDFD"
     gene            42617..42904
                     /locus_tag="IW819_000111"
     CDS             42617..42904
                     /locus_tag="IW819_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243249.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEILDVSMIPSET"
BASE COUNT        16248 a         6145 c         8716 g        12590 t
ORIGIN      
        1 atcaaaaatc acgatattat ctgtattgaa gacttaaaca caaaaggtat gttgcgaaat
       61 cataaattag ctaaaagtat ttctgatgtt tcttggtcaa agtttgtgac taaattacaa
      121 tacaaggctg actggtatgg acgaaaaatc atcaaagtag ataaatggtt tccgtctagt
      181 cagatttgtt cagagtgcgg acataaagat ggcaagaaat ctcttgaaat tcgagaatgg
      241 acttgtcctg tttgtcatac tcatcatgat cgtgatatca atgctagtat caatattctg
      301 accgaaggtc taagactaca ttctatggga ttggcttaga tccaatcatc aagaaccgta
      361 ggaactacgg ggatagcttg gtaaataaga gacaccgctg ttagtaaaga aatacgctat
      421 caagtatgct ctattcccaa gaagctccca cttcaagcgt taagaaccgt caggtttagc
      481 taagtggtga gtagttcaca tagaattctt tattatcaaa aattagggca ttaagtagtc
      541 tgaaagtact ttccctatct ctatctgctt cctcatttcc ataagataat tgaaaaacaa
      601 acttaaaggc tgtcttacat aggttttctt tggctttttc gcttagttta tttagatagc
      661 tattttttag tgcagagtga aattcttcag gtgtaacaga catagcatgt atgcgacttg
      721 catattgtat aatttcgatc gttacttttt ttcctaacat tggatttttt aggaatataa
      781 tttcatagga cttccgtata acatcctgac aagtttcttt tgttatatct cttaattccc
      841 aagtattacc gttgtaagtg actacaggat gagctgaata atttctttga tccttaatat
      901 attttaaatc ctcatattca tagctatcaa ttagctcagt tgaactataa gctttttcta
      961 gtagttccat ttcccatttt ggagagtttg gatcactttt tcttaatcta gctatatctt
     1021 ctaaaatttt cttagaagta ttatcctcgt aaatatttgc taaatcagat aatttatcaa
     1081 ttaaatctaa aataagcgca ctgtaattga cagctattgc agaacgataa tttccctgat
     1141 aaaaactact tctaacttct tctaataatg gaataattct aggattttta acttttttta
     1201 tttcatcatc taatataaac atataaatct ccttttttta tttgtatgac aagtttacca
     1261 taaaaaaaaa ataaaaagcg agacatattt atgttattat gttatcataa taacataaat
     1321 atatggagtg agatgatgaa aaaatggatt tgaaacaatc gttttacatc tggttatatc
     1381 ctgataatcc tatgtcaata atggctgata tttttgtagt tagcatctta ttgtatttat
     1441 tttatctaat tttctttcac aatttaatcg ggtacatgat tcttgattgg aaaggaatgt
     1501 tcgatataga gagaaaacca tttgctaaca atcaaaccta taccgtcttc gaaagtggag
     1561 attgggaaga aaatgaagtt gatgtgatcc atgaaattgg attagcttta tcaggaatac
     1621 aagatgctga tgttttggta tcaatgacaa cggatactgg atacaacgct tatgaggtat
     1681 tttatcttat gtattttata tgtcgtgatg gtcaagaaat tatcaatggt cgtgctgaaa
     1741 aaacaatcat ttctaatact gaaatttctt tctgtatggg tgaaggggaa atgattgtat
     1801 ataaagatgg gtcagcaaac attaaaacag cctttggtga tattgataca agtattaagg
     1861 aacttaggga atatgtgcag aacccatata attttcaata tttagcaggt agaattaatt
     1921 tagtttagga gtaaaaatat gaaaaaaatt attaatcaac caacaaatca cttgataatt
     1981 gaaggtgtta ttttcgtaac aatgtatttt actgtaggaa atggtatctc aaatacaatc
     2041 attcaaaaaa atccacaata cttattattt agtctttatg cttggattgt tcaaatggtt
     2101 tgggtttcta tattgatgtt gttgttttat tggatagtac gggttattct tttagtgtta
     2161 aacaaaagaa aaaaggatag gtgaataaaa tgaaatcaac taaattgaag gttgtattgt
     2221 tgtcaacagc agcattaata actcttacag catgtggtac aactaatagt gataataata
     2281 acaaagatac aaagtctagt aatacggtgg ttactaaaaa cagcactaca tcggcatcaa
     2341 caacaatgtc ttcaatgatt gagaataatg ctgaaaagta tcaaaaatac gaagacatta
     2401 ataaaggtca aaagccggaa gaagtttata ccgactttat taatacagta aaaaatgatg
     2461 aagattatgg gaatgatcca ttgcaaaata aaaatctgac tatcgttctt tacaaacctg
     2521 attgtccaga ttgtcaaaaa gcagaatcaa aaatcatgca ggaaataaaa gaaggcttta
     2581 gttatgctcc aacattaatg catgatatta ccataaataa accagcttac gagtatcacg
     2641 cagttgctgt agatattact gatggtgtac ctgagtgggt acaaaacatt catggtctgg
     2701 ctgatgagaa caatcaatat catacgccaa cagttgcagt agtaacacca aagaaagtta
     2761 atggcacact atactgggat gtaatgggaa tgacaacaaa tacaactgat gaaatgattg
     2821 aaagtacaac taaatattat atggatttgg gagcatataa attatatcaa caatcagaac
     2881 ctgttatgga cgatcaatcc aaagttaaat agttctttag ggcgtaagcc ctattttttt
     2941 aaaattaagt gataacaaat aaatgatttc tgaaacaaac gttcttactc aactactcga
     3001 aacacacaag ctaattaact agattataaa ggagaatgcg taatgaaaga aatcagtgca
     3061 aatgaagctc atattccgca aaagaatcag gttggtatat ataaggtgct tcatggtgaa
     3121 ggaatttata aaatacagtt acttaccggt tacaataacg gtgcaaatgg gaagtggtta
     3181 aaggcaaaaa gtattcgtga acttaaaaat gaagtagaaa aaataagact tgggttaaca
     3241 agggaagaaa taagtagact gagtgaaaat tcttcttttg taatccaaaa agaaaaggag
     3301 aaacatctac ttcatcggga actaataatt gaaaaatact acgatgttga tttacctgat
     3361 cagttaagaa ttattgacga attttcagag aaaaagtgga attctgaaat atacccaagc
     3421 ttaattcaat acaaggcatc aggaaagccc atgtataact acacaggttt tttaaactat
     3481 acacaatata aatatgcctt tcgcaaatta aaaggcatta aacaagctga cattgccctg
     3541 tatgagatga aaaaaatgga tagatcaaaa ttgattaaag agttagtgca aaatacatgg
     3601 gatcaagcga ttgtcgaact agaaaaagaa aacggaaaaa ttttagctaa taaataccta
     3661 atcgtaaatg aaattgaaga attaataggt ttattttcat gtaaaaaagg tgaagttttc
     3721 ttaaaatcat taccaactta ttcaatttta gacagtccgt atcagcttat acaggcaagg
     3781 agatgagtta aatgtctagc ttgaaagatg attatatcca agcaaaacaa gagtatgaaa
     3841 aattaaatat gttgaaatct gaaaaagtcg aattgttttt gactcaaaaa ttagcagagg
     3901 agcatatttc tgaaatagat gcaaaaactt tagagaggta tgaccagcag tttaatcaga
     3961 tacatcacaa catcattttg cttgttgatc aggcaaagag aagacttgaa aaagcagaat
     4021 atgatttatt agatcaagga attgtatgct tggataaggg gatacgaaga tattatagtg
     4081 cagagttaaa aaagcaaaat gtagatagaa atgcactaaa aaatgaaatt ttagaattga
     4141 taatgcaacg aatttgaagg gatagaaagc aaatgtcaca aaacattagt gctgtaaaac
     4201 aattaataga agaagattta gagactacag ttcagagatt agcggaacga atacattttg
     4261 caaattatca aaccatctct aactggtcaa gcaggaacag aaatccggaa gaaatcccaa
     4321 tgaaattttt ttatcgaatc ggaaaagaat atggtgttga tccgataaaa attttcgaga
     4381 agaccatgat gtattactat catggaacac ctaacgaagt gttagaacat cttttggaag
     4441 aagcaaaaga aaaatcatca atattagagc tatcgcaaaa tgaaaaagtt attcaagtga
     4501 aatgcgttga tccaacagga aagattattg tcggttttga tattgaaaga aaatctaaaa
     4561 tagattttat tactcaatta aaaaagataa ataatttaga tattctaacc cttagaccag
     4621 atcatgaaca gttgtttatg agttttaaaa aacaattgaa agaaatacaa aaaagatata
     4681 acaaggtgtt tcctaatgcg tgaaaagctc aaagaaatca gagtgttttg gataattaac
     4741 aaagaattta tcatcttata tactcttgga tttctcagca tgatcttgtt ttggtggtta
     4801 tatttaaaaa aataaaaaga aggaagaaaa tatggcatac gaaaatttac aattagtagt
     4861 tgcaggagtt agtggggata tataccttgc aaaagcaaaa gatggaatta tggacacgaa
     4921 ctataggaga gttgctacag atgatgtgtt aagttcagcg acagaatggt ttcgaaaaaa
     4981 tgaagaatct gctgttcact ttgaaggaat tgatggaaac actcatagtt tgttctatac
     5041 aaaagacaaa gaaaaagcaa aaaaaataat tgatattttg aaagaacgtt agttaattaa
     5101 ccaacgatat aagagaaggt gagaccaagt atttgcttgg tctctatttg atagaataca
     5161 tattctattt aaaaaaagaa aatcattgat attatacaca ttagtgtgta taatcgagag
     5221 aggggtgatt agaactttga gcaattcaat cattgctttc gtaggagaat aatagaacag
     5281 ttcctaagaa gtccattatt tactcattag gtaaagaaag cagatattta atacctaata
     5341 ggtacttaga gataaagagg aatagcgatt acaccaacta tacgctaggt gagttaaaag
     5401 ctcttagaca gagcgacagc gaggaattta gcacgctcaa aaatgaagta agcacagaga
     5461 attttcgcat atcagatatt gaagaattga aaaaacagca aaaacagcaa ctagaaaagc
     5521 aaaaaaaatt aattgaagac attgcatata aacgagtaga tgagcttgca gtacaagaag
     5581 ctaaaataga aaaattgcga caagagctct atgaaaaaca gcaatcaatg ttagctgaag
     5641 cgaataaaat gcgctttgaa ttggagaaaa aagtagaaga gttaaatact aaaatttttt
     5701 atttggaaac tgaaattgat gcgattgaag gctattttgg tgaaagtaca acttttgttc
     5761 agttgcgttc aggtcaaaag agcttaccgg aagatccagt ggtgatgttt caaaaagtac
     5821 ggtatctaga tgaagaatta ggaagatatt tcagtatgcg agaagttgaa ggtacagctg
     5881 ataccatgct gacttttgag caggcgctat cctcacgaga tgatttgttt gatttattct
     5941 caccgaacaa aaagtcaatc tcaatgatac gtatttccaa atcaggatat ggctacacta
     6001 acggtcaaat ggaaagtaca aacatgctag aaaagtataa aaaatatcat ggagaaactt
     6061 tagcaatttt actgagaaac ggtgataact tatggatttg ttggttggat gaaaataaga
     6121 tcagtttcaa agatgatttg ttttaccaaa acaaagtaac agagcaggca tctgaaacca
     6181 aaaatatggt aaaggagata gattggacac atactcgtaa tttagaagag cgattgaaag
     6241 atgctaaaaa gcagaagagg gctgttagag gagagtttgc tagtcgttat attctttttt
     6301 caatcttaca aggtgttcta gataatggaa cgatgatgac cttaccagaa aaagtaaatg
     6361 tgatccaagc aattaatcag aaaagcccat attttattgc aagcgtagcg gatggatggt
     6421 tagaagacac acgcttgccg tcatttagcg agttgattga taaatacagt ttctataaag
     6481 aaacaggtga agacagagta ttagaagcgg aatatgagtt gaatcccgat aaatttgaaa
     6541 atatgagaga ttatccacac aaacaaaatg atgacattta tgtgttatac aggttacgtg
     6601 ggtggtcgcc tacatcagca aattatactt attacaatag tgataaccgt tcaaatagtg
     6661 agagtggacg tgacttagca tattcgattg acttgaaaga cggcatttat cagattaatt
     6721 tagtaaaaca gggaaaattc caaaaacaaa tgttttcccg aacgtttgtt ggagttgatt
     6781 ctactacagg cgaaaatatc tatgattact attatgaaag agaaacagag tactatattc
     6841 gaggacaaaa agcctacaac aaaagtgact attggggtga caaggcgaag cgattaactg
     6901 caaattttag agtgtatgaa tccgaattta taaacttaac ctttcttaat agtgttttta
     6961 ttgattatgt aattactacg aaacgtattg gaagttattc aagtacctac gcatccttag
     7021 ttccaatgtt aaactcgatg agccgtttct taaaagaaag agaagagaaa gagctacagt
     7081 atatcaaaga aattatgcca gattttgaaa tgcacgaatg gcaaattgct ttatcagatt
     7141 ggaaattgac gaataaggtt cgtagaataa caccttttca agccaagcgc tttttgaaag
     7201 ctatgaaatt tgttcaatag aaaaatccac cagacaagct gatggaaact aacccaatta
     7261 ctaacagttg taattggtat tagcttacaa gttaattatg gtgtttagtc aatcccaaat
     7321 tgtgaaacgg caccaataat tggaaaggaa aaatgaaatg tctgaagatt tacgagagct
     7381 gaagttacga aagaagcagt atgatcagta tttttccggt ttattgaata aacatattga
     7441 agaaggactt gatcatgatt ttggaaaaga tgaatattgt ttgtattgcg agcgttacaa
     7501 tgaaaaaatg tctgaatatc tacagctaaa gaaagagata caaaaattag agaaaaaagt
     7561 acatgcttgg aagtataaag attccatgtc tggttatggt gaatctcaaa ataagccaag
     7621 aaaaaagaag caagcgtatg ttctaaagat ggtaaatggg gaagagttaa ttgtattagc
     7681 ttacagtaaa gaagaggttc gagaactcgt tgaaacatct gtcaggaaaa agatagaaac
     7741 aataaatgag atagacgaca gccaatacaa tagcatctat attgaaacaa gtcatggtgg
     7801 atataagaaa ttagcaactt tccttagaag ttctaaagtt gggataattt ctaaaaaaga
     7861 cataagtgct gttaccgata gtttttgtac tttatctgtt cctaaagcat attttcaata
     7921 caacagtaat agatttgatc gtttagagaa attctgtaga ccatatactg tattgaaaac
     7981 taaaaaattc aataaaacta aaagagtttc aatcaagtta ccgactggtc aaatctcgct
     8041 aggcgaaaaa gattatctga taaaagtagg aaaagagtta ataatcaaag tgccggaacg
     8101 cttatttaaa gaaaattttg agatacgtga aaccttggta tggaaagaag aataatagtt
     8161 tggaagacat agcagggtat aaggaggaat taaaatggca gtaaatagaa aattgcaggt
     8221 attgattaca gacgactttg gcggatcaga gtggtttgat gtgcagtcta tagattttga
     8281 agaagaagtc gtagttatag aaaatgtata cggcgaagaa tactcatatt cgcttgaagg
     8341 agagaatgtt gaggatttac ggttgattgg tgagtagaaa atagcagaaa tatcaaaatg
     8401 gagatgaaat cagcgtgtat atgattaaca agaaaacagg aaaacgtatt ttcagcagta
     8461 aagacgaggt aattcgtgat ttgagtgaaa actttgaacc aattgaatta tttttccatc
     8521 agttaaatct agaaattgaa aaattactaa aggaacctac agaaggacga attaataaag
     8581 tattgaaatt aatagcatgt aaagatagtc atgcttacat gctttatcca aaatggtatg
     8641 cagacaaaaa tatagctgaa ataaacgact atctagctag tataaaggaa cgcaatgcaa
     8701 ttaattaaca atatacgaaa aatgaaatta aaggagatag gaaaatggca gaagttaaat
     8761 ttggatcaaa agaatggttt gaagagttaa actcaatgat tcagtttcca gaaaagttca
     8821 ctttttacta caacaaaaga gacaatatca ttcaagtagc tttacttgaa actaagggaa
     8881 gattagaaaa tactcgatgg agtatgataa aaccatcagc actcagattt catcctaaag
     8941 acttgacagc ggttgtagcg gtaaaagagg ttcaagactc acaagaatac tatgaagtag
     9001 taaaaaatct gaaggatatc tggtgtaaat aattctaaat tgaccagtta tttggtcaaa
     9061 aatagtatgt gattatttca tccaatatgg catcataatt gggttaaaaa tgttaaaatt
     9121 caaaaaaatg agtcagaagg gataaaagta tgaaaagaga ggaatacaaa caaagattaa
     9181 acgaactgct tgaagaggac gaaacattaa ctcatggttc gcctgatgaa attctataca
     9241 tgattgataa tatggtgatc tttggtggat atgaacttgg taataggtca gttgatcata
     9301 atattttaga atttgatgat gtatcatggg aagaaattct tgattgggga attttggcgg
     9361 tacctgaaac taaaatctat attagtgaca ctatggttcc tttttttgag gaattggatt
     9421 ataaaagact tcccaaaaat gaaaatcata tactgggagg aaactaacaa tgttaagtga
     9481 cttatttatt ttactgatct atctaggtgt aagtgaaatt attgcgtatt ttttgtttgt
     9541 cattgctaat tatggtattt ccagtgcaat ctttcaaagg tttagattac cagtaagtga
     9601 actaaaaaaa aatgatgaga ttatcagcat atcttttaat gtttttgaat gtggtcacgt
     9661 taatctttgt agtaatcggt gtgactttca atttaatgga atagagggaa atctataatg
     9721 gaaaaaagag aaaagaatga acatattttt tgaatcaata ttgttgatgg tttttgcgat
     9781 cagcgcttac cagaaatcga gtagagggga aagccaactg accttccgcc cctctcacac
     9841 caccgtacgt acggacccgt atacggcggt tcaataagtt gagtgtacga actggtactt
     9901 caaggagatg tcctcatatc cttggcgggc cagttctttg tctgttaatg atcgatgaag
     9961 aatatgactt ttggagactg accaaatacc ttttcttgta tttgaccatt catacgcttt
    10021 cgctttatcg atccctaaac gaatcagatt ttttcttttg gttcttggat tcttccattg
    10081 tttccaaata tattgtctga ttcttcgctt caaccaaccg tttaactccc tcatgaattc
    10141 tttcatctca ccaattccat agtagttgat ccaaccagtc attagttgct tgatttcttt
    10201 taaaataagt tggatacttc ttccacggtt tcttttagtg agttgtttga gcttgctttt
    10261 cacacgctgt tttgcttttg cgtgagggcg aattttaata cctttctttg ttggcagaat
    10321 gcagaatcca agaaactttc gtttcaatgg acttcctacc gcactctttt ctcgatttac
    10381 cgtcaacttt agttgttctt cgagaaattg aatggatttc ttcatcacac gctgtcctgc
    10441 tcgtttgctt ctaacataga tattgcagtc atcggcgtat cgaacaaatt tatgtcctag
    10501 attttctagc acttgatcaa attcgttcag gtagatgtta cttaacaggg gagagagatt
    10561 gcctccttgg ggcgttcctt cttcagttgt tgtgatgagt ccattttcca ttacaccact
    10621 cattaagtat tgacggatca gttttaggac ttgtttgtcc tttactttcc gctcaatgaa
    10681 atacatcagc ttatcgtgat ttacggtgtc aaagtaggct ttcatatcaa tgtccaccac
    10741 atatcggtaa ccttgttcat aataagattt tgctttcttc actgcgtcat gtgcgctgcg
    10801 atttgggcga aacccataac tattatctga gaattctggt tcaaatagcg gttgtaacac
    10861 ttgattgatt gcctgttgaa gcatacgatc aataaccgtg ggaattccta gttttcttac
    10921 accgccgttt ggtttcggta tttctactcg caaaacaggc tgaggtttat agttcccttg
    10981 gcgtaaattg gatagtaatt cctcgcggtg ctctttcaag taaggaagca gttgatcgac
    11041 ggtcatccca tctgcaccag ccgccccttt atttctgact acttgaagat acgcttgatt
    11101 gagattattc cgatctagaa ttctcccaaa cagattttct acaccatctt gtttcctctg
    11161 ttcaccgcaa gaagtgctgc gcacttcatc atacccttcg tgttccacac tagctctttg
    11221 atgatagcca agttttcttg ttttctgcgg tcgtcgcatc ttctcacacc tcctaaatta
    11281 ttcaagattc ttattgttca gtccttcgta acggtcagtg tcgttactac tatgacctcg
    11341 gctgacttct catgattcac catcatgtcg ccatgatcgt tgtgctggtt tgttttcatt
    11401 caacgcccac aacaccctca tgagatctcc cccggtaaga gtgaaaactt tcctcccatg
    11461 taactgctag atttactgta taggtttcgg gcagtattgg actttgtctt gtgatgaaga
    11521 cttatccgac ctaattcagc cttgtatcta gtttctgttc gtcagttcag gagtttgcgt
    11581 ccgacttcct tcagccacaa cctcacgatt gccgccttgt ctttcgctaa cagttcctac
    11641 tgccaagcct gtagtggact ttcaccacca agttttcacc catgcagggc gcacatctaa
    11701 aaaaagagta atgattgttt aatcatcact ctttttttgt agggaaattg aaatatgtgg
    11761 agcttgtatt ggtttagtta ggattgcttg gctataagga gctagttctt tatcaaaacc
    11821 atcaaagtag gttttcagta gtaatttaac tgatcgctca tcttgtttga tttggataat
    11881 ttcagcatcc aaatcggttg tgagatcagg agcttttgat gcgattacct gatttttttt
    11941 atcttccaac tctcttaggc tgtttaacaa gttgtaaaaa gaatcatttt gattgttcat
    12001 tccaacgtga gttgcaattt taacagaagt ttcgtccgta ttgtcatcaa aagaactcat
    12061 tggaatatca agtagtaacg aggatgtttc ttttactgac tttttcttta tagcattttt
    12121 tgtttttcca tcttttttgc tctttactcg agatgttttt ggagctagtt taggtaaatc
    12181 acttgtttta tcagtcattt attagctttt cctttctatt aatttatgtt attatattat
    12241 cataataaca taaaaaagta cactttttgg gggcgtagtt attgaaaaaa tctaagagaa
    12301 agattgccac gttgcttatg ttaatggtgc aatttacagg gattgcaatg agtgtaatca
    12361 caataaatcc gcaagtgata catgctgatg ggatagctac ttatgatgca caatttaact
    12421 gtactactga aggtagtggg tcaggaacag atagcgatgg aaacgatact ggaagtggcg
    12481 ctgtaacagg tgatgtagcc aaaaatattg aagatattta caaatacatg aatggagaat
    12541 atggcttatc aggtgctagt attgcaggta ttttaggtaa ctggaaacaa gaatcaggta
    12601 ttgatcctcg agcagtagaa gggtattacg gagatggaca gagagaagtt gacttcatca
    12661 aacaatttac tgatggtctg atgggtattg gctatggtca atggacagct gggcgacata
    12721 cagcattagt tgattggtct aagaaaaata atggcggttc ttggtggact acagaagccc
    12781 aattggactt tatgttcaaa ggtgatgggg gattcgtagg aatattaaaa gattatgctt
    12841 taaatgcttc tgatgatgtc gtagaaaatg cagtaacttt tcataaaaac tgggaaattt
    12901 cagctgattc cgaatcagct gtaatagctg tacgtggtga aaatgcaaaa gaaatctgga
    12961 agtacatgaa aagcaagggt atggacggtg caaaagatga aagtaagatc aataaaatat
    13021 ccggaggttc aagtaccgga gatggatcga ataattcatc aagttcagca tcaggaacag
    13081 tagaagatgc ctgtgcaact acggatgacg ctaattctgg ttctggatca attgggaaag
    13141 aaggaaaaat taacggtaaa aaaggtcaag taatagcgaa tatgacttat gaagaagctt
    13201 tgaaaaaata tgggaaagcg atcacgctac caaaattcaa agaaccagat tggtcaactt
    13261 caccttttaa cggaatatat caaggtcagt gtacagagtt gacgtgggct tatatgagcc
    13321 aattatacga tgggaatcaa ccgactactg gaaatggtgg ctatgtatgg gaaagctaca
    13381 aatcggcagg agcaaaaata acgaacaaac caatcgtagg ttatgggttt agtgcttcgg
    13441 atggatggtt atgggctgtt ggagatgcag ggcatactgg tgtagttata gcggtgtttg
    13501 aagatggatc atttctatgt gctaacttta atgttccacc aacccaagct ccaacaaggg
    13561 gagtttcggt aacgttgatt gatggagtag acggatcaga taatgtgcat ttctttagcg
    13621 gtgtaggaaa agctaaattt gaaatcgaaa aataaattgg aatgaaggac gatatagatg
    13681 aatagtaaag tgaaagatac aattatcatt tctttgcttt ctggtatgtt attagcactg
    13741 cttgttggaa tcctattctt caataagcag aatcaggaca tagaagctga aaaacacagt
    13801 gtgcatattg aagataagct gacattcttt aaagaggtta aaccaaatga ggtggatgga
    13861 ttacggaaag atttagaaaa tgatattcaa gaaatgttga atggatcagt tcaaaacaag
    13921 tttgcagatc aatcaggtga agaatttttg agaaaactat ttattgtcac aggtaaacca
    13981 ccaattactg ataagagtag tgagaaagaa aaaatcgagt actataagcc ttttactttt
    14041 aagctaacaa actttggaat taagacatct agtagtgggt tgtatgaagc attgaccgat
    14101 tttgaagttc aatacaatgg aaaaaaagtg tatgatacca cattaaccat cacactaaat
    14161 aatgataaga aagtgactgg gggtgctttt tatgcaaaat catgaaatca ctactgctaa
    14221 aaaggcgatc ggaatacttt ccataattgt gatcctttgt tgtgtggtac tatttcaaaa
    14281 gaaaaatgct tatgataata atgtcaaaga attggaagac cttaaaagta aacaaatcga
    14341 agtagtcaag aataaccaga aagtgaaagc tatgcaggaa gctcaataca aagaattggg
    14401 aaatgctgaa atcaaggaac aaagtaggaa attctttaat ttattttaca actggaattc
    14461 atgggaaaaa tatacgaaga atatgaagga aatacaaaaa gaatttcctc agatagtaaa
    14521 gtctagtcct gttgatattt ctggcacgat ggtaggcact ggtaatagtc caatatctag
    14581 ctatactgct gattactata caacaaatca aaaaggtaaa atggttgaat tgattacaca
    14641 gacaagaaat actccaacaa cagcaacaac aacaatttgg cgtggagtaa caagcatagg
    14701 agaaaatcaa ttgttttact tggaggattt agatagctat gtaagagcag agtaaaataa
    14761 aaaaagagct aacggtacca acgttagctc tttgtgctga cgatgataac aaaacctacc
    14821 gaaaaaggat tgtttcatca caaacacaaa cagaaaagaa ttgataaaaa acccgaacct
    14881 cagaattttt attaatcaat tcatttagat tataataaat tgagttgaac attacaatac
    14941 gtatatgttg taaaacgtaa aaaaagtcaa aagtaaatac tttcttaaaa agtcaaataa
    15001 aactattaaa gacaatatat atggtttatt tgacataaga aaatggtcta gatgataatt
    15061 gtaatataga attgtagact taaataaagc ataaaatacc ttttatgata aggtatagct
    15121 cttaatttta agcaggttta gatataagat taaaagtaaa gtgggggaga ataataatgg
    15181 cagatcgtaa attagaaaag ttattagaag aaacgtggaa tccaaaagaa tttagtgaat
    15241 tttttatgga gaattttgaa actgacttag cagtgattgt taaagatgct cttagagagc
    15301 aagggtatcc tgaaactgca aactatataa acattaattt tactttgtat actgaaaata
    15361 aaggaacttg ggatttttgg gctactttag caaataagga gttaagtgat aaatcagaca
    15421 caggaattcg taactttttt gaatcaaaca gagatgatta tatgtatgct aacaatcaaa
    15481 ataaattgaa ctttagagta gagtttgatg aatcgcctga agaatttata gaaagacaac
    15541 cgcctaaaga aaatgtagca aaggtattgg aagatcgttg gaattcagat gaaatagttt
    15601 caacaattag cgaactaggc ggacaatatg aaccgctagt agaagcggtt agagaagagc
    15661 taagactgaa taagtttcct gatgttcaaa atattgatgt ttctcaaatt gaaataaacg
    15721 tcaagatcac gaataaactt gattatggta gttgggcaga tatagcatta gaaaaataca
    15781 tctactcaac gttaaaagag tttatcgaaa atagaatgga tatcatgtat ctacagcatc
    15841 cacagtattt aaactttggt gttgaaatag ctacacctat tgaagaatgg aatatggagc
    15901 aagggcttga ttagcctttg ctccattttt ctattttaca aatgaataca tttcatcatt
    15961 tttaccaaga atgattgttt gcccaacttt gagattgtca ggtgatcctt cagcggtttg
    16021 taaggagaac gttccattga atttatcgga taccgttata ggtagcttta attctgatcc
    16081 ttcctcatct ttgagaattg ctgttttccg ttcatcaaaa taaacagtct ttattaaata
    16141 atttttcgta gtgatctcag cagatttgtc tttttttgta gtagtaccag tttctttaga
    16201 attagaggta ttttctacga ttattccatc tttggcattt gagcttgtgt ttatttgatt
    16261 gaatattttc agcgacaaga taccgctaag aatagtcaat atagaaacaa gtgtaattaa
    16321 tactgataat ttcctttttt tgttcatttt tcttaacttc cttttttgtt ttgattagtt
    16381 agatattcct tatctaacaa gcatttttag gctattaaat gaagacaaac cccaaaaaaa
    16441 cattgcaatt atgacagtgc aatggtacta ttattataca ataaattttg cgaaaggagt
    16501 aggtctaaat cgcaaacaaa aagaaattga acctagacaa atacaacttg tataccgcaa
    16561 gggaagcaag tttgattttg ggtaaaggta gaaattatgt tacaaacata atcaactatt
    16621 atccaaatcg tttgcctaaa ggttcattca agaaagtggg tgacaaaacc tatattataa
    16681 cggaagaagg aatagaagca ttgcgatcac aaacagaaaa taaaaacaaa aagaaggaat
    16741 gaatatgaag aaaaattcgc ttggtatggt tttatttgca tcagctgtac ttgcggggtt
    16801 atcagtacca gtaacctcac atgctgatga atggactgca aacacaccgg atcaaatcca
    16861 aattacaaca ggacaatcaa gttatgcgtt caaaaaagga gatactattt ggtcaactgt
    16921 agagaagctt aaaagcttag gttacaagac tactgttgct gagtttgccg aattgaacaa
    16981 tgttgatttg gcaaaaggag aacaacggac tatcccaatt ggacgaactt tcaacatcag
    17041 taaagataca ttaacagtta aggaagcaga tggcgaagta gttagtcaaa ctgttcaaaa
    17101 ggataaacct gatcctgttg aaaatacgga gcaagagaac aaaaaatccg agactacaaa
    17161 agaatcggaa ccaacagcaa acacttctga taagacagaa acaagtgctt caagtgttga
    17221 tggagcaagt tcatctaata caaataattc tacaagtgag aaagaagcat cacagggaac
    17281 aagtgaaagt agcactacgg aagctggtac tggaacatca acaaataact cacaatcaac
    17341 atcaaatgat gcaaatgaat caaccagtac atcttctagt gatcaatcac aaacaggttc
    17401 gagtgatgaa actgacagtt ctacttcacc aagtaaaccc gaacaaccaa ctaatgttgt
    17461 gctaggcgtt ccatttatta gtcaaggtaa cacaatgctt tgcgaaggaa catctttgct
    17521 agaagcgtta cattataaag gtgttgcaac aaatcaagat ttaatgactt ttgttaaatc
    17581 aatgccacta tctcctgata ataatccaaa taatggtttt tctggtgaat ggagacacaa
    17641 tgtggatgga acttatcaag gtatgaatcc ggctcctgta gtttcatggg gtattgctaa
    17701 tggcggaaat gttgtagata tttctggttc atcggtacaa ggattaaaag atgagattac
    17761 taatggaaac cctgtagtag cgtgggtaac ttatgccttt gaacctgctc aacataaatc
    17821 aatgttctgg ggcgatgcta tttggaatag acatgtagta gccgtggatg gatttaaaga
    17881 cggtttttac catgttgtcg atcctgtgtt tggggcaagt tggatcaatt caggatcatt
    17941 tgaaacagca tataacgtat ctcaattagc ggtttcagta cgttaataaa agagatcgtt
    18001 tgtatactaa gtagttaagt aaagtatttg tttgtcattg cttaactact tagtaacttg
    18061 ttgctcacgg tgggctagaa ctaaaagggg tgatgctatg aataatggag cgttgaaaca
    18121 gctttctttc aacgacaaaa gtgattaata ggtgtggtga ttttagctta ggaatgggcg
    18181 cgaagtcatt accacaccga catatcgtga ctcattctct tcgcctaact ggtataaaga
    18241 aggagtaacg agagatgaag catgaaaaaa cgatgatttt aaattcaaaa ataatttatt
    18301 gttttgcgac tttagttatg gtgttatcta cgcttataat gcctttttca gcaataagag
    18361 ctgaagcaga tgtcacaaaa ccgacaagtg caaaatttac agaaactggc gtaaaaacag
    18421 acggttatat tagagttaat atgccgaatc atccaaatga atggatgatt tcaagtcaat
    18481 ttaaagattc tcatggaaat attggatatt gtttggattc agaattgcct tccccaacag
    18541 gaagtggagc cggaagcttg aagtacaagg gagccggcag tgatgagttc tatcgtatgt
    18601 ttaaaggtgg tttcccatca aaaacagcaa aggaactagg ggcaggaaat gatacagaag
    18661 catggtatgc aacacaatta gtttcttggg ttttagccgg aaactttaaa gtttcgcaga
    18721 ttgtatggtc acatccaaac cataccgccg cagaaacagc tcgtgtaaaa aaagcatttg
    18781 aaaaaattta tgactatgct aagaatggaa aagatacgcc caacacagaa ttttctatca
    18841 cagcttctaa gacagctgac gaaggaaaat atcacacttt cacatataaa acatcatcaa
    18901 ataagactgg taatgcgaaa ctcactttta cctcagcaaa accagctggt atgaagattt
    18961 atgatgcaga tggcaaagaa attactaata acaccgtaaa attaaattca agttttacta
    19021 ttaaagtacc tgtgactaca ccaagtggta ctttgtcatt taaaggtact gctaatgtta
    19081 gcacaaccaa tccttttact tttgatggac gaggtgtgta tcaagatgcc gtagttatga
    19141 tcactacttc agaaacaaag gactctaaat ctttgagtgc taaatggaca agagctgttg
    19201 gtcaggtaac agttaagaaa gttgatcaag atggaaatgc tttaaaagga gcggagttca
    19261 ctctaactga caataacaat gctaaatctg ttaagacaac cggagatgat gggttagcta
    19321 cgtttgattt gcaacaaggc ttaacataca gcttagaaga aacaaaagtt cctgaaggtt
    19381 atcaaggttc atttaaaaag gatggtatta ctgttaatac cgatggacaa gaatttagtt
    19441 acactgctac aaacacaatt aaaaaaggta aagtaaaagt taaaaaggtt gaccaagatg
    19501 gaaaaccatt agctaatgct gaatttacct tgacggatga ttcatcccaa acacaagcag
    19561 aaaaaacaga tgaaaacgga cttgcaagct tcgatattgt agcaaacaag acctacagcc
    19621 ttgaagaaac cggagtacca actggttata caggcgcatt taagcaagaa ggaattaccg
    19681 ttaaagatga tggacaaact tttgaatata ctgctacaaa cactttgaaa aaaggagtag
    19741 taaaagttca taaagtcgat caagatggaa aacctttggc aaatgcagag ttcaccttaa
    19801 ctgatgaaaa aggtgaaaaa aaagttaaaa caacagatga agctggtttg gctactttta
    19861 atatcgtagc aaataagacc tatagccttg aagagaccgg agtgccaact ggttatacag
    19921 gcgcattcaa gcaagaagga attaccgtta aagatgatgg acaaactttt gaatatacag
    19981 ctaaaaacac tttgaaaaaa ggagtagtaa aagttcataa agtcgatcaa gatggaaaac
    20041 ctttagcaga tgcagaattc accttaacgg atgaaaaagg tgtaaaagaa gttaaaaaga
    20101 cggatgcaac tggtctggct acgttcaaca ttattgcgaa caaaacttac aacttagaag
    20161 aaacaaaagt accaacaggt tacacaggcg cattcaaaca agaaggaatt accgtcaaag
    20221 atgatggaca agtctttgaa tatacagcta aaaacacttt gaaaaaaggt gtgatcaaag
    20281 ttcataaagt ggatgatcaa gggaaagcat tagcaaacgc agaattcact ttgacggacg
    20341 acaaaggtgt aaaagttgta caaaaaacgg acagcaatgg cttggcaaca tttaatattg
    20401 ttgcaaacaa aacgtacaaa atcgaagaaa cacaaaaccc taatggttac acaggaaaat
    20461 ggtctaaaga aggcatcact gttgctgacg atggacaaat ttttgaatat acggtaatta
    20521 accatcgtgt acctgaaaca gtccgcacag gaggaatcgg tacatcagct tttcttcttg
    20581 tatttctagt tgcaggattg ggcatggggt tgttgtatta tcgagaacaa cgcaaagtaa
    20641 aataaaaaat cggtaatgga aaagaagtaa atatgaaaaa agaaggctga cgtatgaaat
    20701 cgaaaaaaat tttttcaaat ctaatgctat ttggtacatt gctaggagtg ggattgggcg
    20761 caagctcaat ctcttctagt tttgggcttg gggagcaagt agcatatgca caacaaaact
    20821 ataattatca aggaactgga actattcata tccataaagt aacaaatact aatggggatg
    20881 ggaaacctgt ctcagatatt gataacaaag ggaaagaaat agctttacca aatggtgtac
    20941 aacctcttga aggcgttgag ttctcttatg tgaaacttac taaagatgaa gctaaatcct
    21001 tgggatggac agatagtgat actacaaaag gtcaacatat tgatgatgct aaagcacgat
    21061 tattactaaa caaaaaatac agtgaagtaa gtagaatgaa gactgattca caagggaata
    21121 tagacatacc tgtacaagta aatacagacg atttaacaga taacttgtat ttgattgttg
    21181 aaacaggaac agtcaaaggt gtagttgaag aagcgtatcc tatgattgtt aacgtacctg
    21241 aactaaacgg atcagatgat caatacttta attatgatgt acatgtctat cctaaaaact
    21301 atttccaatt aaactcagca gaacttgata aaaaagacga agatggaaag gctcttgccg
    21361 gaactgtttt tgaattgcga aaagatgatg ggtctgatca ggtcgttgga acttataaaa
    21421 cagatagcac tggtaaatta actgtagacg gtttggaatt aggtgactat tatttcctag
    21481 aaacagattt gggaacggat gctaataaaa caacctattt actagatgga aaaaaattca
    21541 aatttagcgt taaacgtggg gagactgcta aagtagttct tgatgttacc aactataaac
    21601 gtcctgtaat tactaaggtg attgataaag aaactgttga tactggtgaa gatgttcagt
    21661 atacattgaa accatctatt cctggtaata ttggagatta cgaaaaatac gttgtcaaag
    21721 atgtactagg agaagagttg acttatgatt cttcagaagt gatagaagga atgactttaa
    21781 atactgacta cagcatcgag cgtgacggac aaacgacaaa ctatgtcttt actgattctg
    21841 gtatggataa acttcagaaa aaatttgatg caggggatcg cttctttaca atcttgatga
    21901 atgctcacac gaatagtaaa gctaaaatgg atacaccact taaaaatgac gtttcaacaa
    21961 tctacaacaa tggatatgag cctgaagacg aaacgccagt tgatcatgct tatacaatca
    22021 ttggtgatca ccaattcatt aaaacagatg gggatacaag caaagcgttg agtggagcga
    22081 aattcgtagt gaagaacaaa attgatccta acagagttac ttacctgaaa caagacccaa
    22141 caacgaaagc tattacttgg gttgaagata aatcgaaagc tactgaattc gtatctggta
    22201 cagacggtaa gttccaagtt gaaggtttgc aatataccta tgatgtagat tttgataaac
    22261 aaggtaatcc aacaattaca gataaaaaaa tcaatgatta tgctcttgaa gaagttgttg
    22321 ctccttctgg atatacacca acacgaaatg atgttccatt tgtagtatct aatgatgatg
    22381 cttcaagtaa agtgacagac ataaaaaacc tgaaaactcc tgaaacagtt cgtacaggag
    22441 gaattggaac agtagtattc ctagtggttg caggtgtcgc aggaacaggg ttgtatgttg
    22501 tttacaaacg ggaacaacat aaagcttaat tattaaaaat cccctagagt gtttttggct
    22561 ctagggtttc tttttaaaaa gagtatgaaa agtaggtatg agtatgaaaa aaaagaatat
    22621 caagctaatc atcttaagaa ttgggatgtt gctatgtcta ggagtaggtc tataccctgt
    22681 aattactcag gcttatacct attaccagac tcaacagacg ttaaatgcct ataaggaaaa
    22741 atatgatgaa gcttctgaaa aggagaatga agaaatcttc aaaaaatatt cggaaatcaa
    22801 taggaaacga aaagaaacag aacaaacctt agttacgatt cctgaagata ataacaatat
    22861 tccgtcaaat ggaaatcttc aaaaggataa attgggtgaa ttagtaggtg agattcttat
    22921 accaaaaata aatgtggatt taccgattta taatggcgta aacgattttc aattatcagc
    22981 aggagcagga atacttactg gaactgatta tccgaccggt gggattggaa accattctgt
    23041 catatcagct cattcaggta ttcctaataa aaaatatttt acagatttag aattgctcaa
    23101 acttggtgat aaattcttta tcaatgttgc aggaaaaaaa cttgcttata aagtagatcg
    23161 gataaaaaca attctaccta ccgaatttga ggatttgctt cctgtagcag ggaaagatta
    23221 tgtaacgctt atgacgtgta ctcctgttgg agacaattct cataggttac ttgttagagg
    23281 agttagaata ccctacaaaa aaaatattgt tgagaaagaa aaaaagaaag aagacttaaa
    23341 agtaaaggcg cagataaact ggtatcccat catagttgta ggtgttttat caataatagc
    23401 tttaatagtc attattaaaa aaatagtggc aaagagaaga gggatgaaaa atgaagaata
    23461 aaatactact ttttttgact cttatttgca ctttggtttt aacaatttat cctgcaaatc
    23521 agctctatca tcaatttaaa acgaaagaga atgtggcgga aatccaagag aaaattgaga
    23581 aaaaacaaac tccttcaaaa gaggaagtgg caaaaaaagt cgaacaaata aaatcgaaca
    23641 gcggtaacca aggattagac aatactcaga aaacagacgg cggatcattt gatattggtg
    23701 aagctgaagg aaaggtcaat ccttacacag gagatgtttt ttcaaaaaat caaacgaaag
    23761 attttgaacc atctacctat tcaggggcgg ttggttatgt ctatattcca aaaataaatg
    23821 tgctagagct tttgtttcta ggcgcttcac aggagcattt atatagaggt gtggcacaga
    23881 tgagtgctac tagtctacct ttatcaggta aaggtcatca aacagtaata gcaggtcatc
    23941 gtggcggtta tacgtcacca caatttcttt atgttgatca attagtagcc ggagaccaca
    24001 tttatataga ttatcttggt gaaaaagcag tttatacggt tttccgaaca gacgttatta
    24061 gaaattacgg tcagaattca acactgactg atattcctga tttagatatg ttaacacttt
    24121 atacttgtca tccatatcct cagaactacg atcgtttatt agttagaagt ctaagagatg
    24181 aaaaagcaaa atacatcacg ccacaagtaa cagttggcaa taccgcatac gctgatgcca
    24241 ttgatagcga gtccgtttca gataagacag aaactgttac atcttctaat gatttatttg
    24301 aggatgttga tcgtgccaac aaggaattag atggaaataa tgatcttaat attccggaac
    24361 aaggtagcgt agagatgcaa gacagaacgt ttgctgaaaa ggcaagttca atatttacag
    24421 gaattaccaa taaacatcag aaaatgcttt tagtcatttg gactgtaata ggagcattag
    24481 caagtctaaa ggttcttatt cttttaatca aagagtttcg aaaagcataa tgaagattat
    24541 aaaaattgat agagtacagt aaaaagggat tgatactatt attgatctct tttttatttt
    24601 gaatagagct agattcactt ttaaataaaa tcaaaactga tcttaattct aaagaaaaag
    24661 tcaagaaaaa aatacaacaa atttttttat cagaatattt atcagaatat tattagaaaa
    24721 gataaactca aaagcttaat ttaatagggt tattggttgt atttagaaat aatgtatgga
    24781 aaagctataa agcaatagat aagatgtaga aaatcttatt tgtgaatgaa aaacaagtca
    24841 tcgtaaaata ttaataggag gtgtagtaat ggtgggaata tcaaaaacag ctttatactc
    24901 attagctaaa ctatttagta gcaggtttga tggtgcagaa tttgttgaat atctaagcca
    24961 aaacgaaagt ggaaaaactc tgatcgaaac aatgaaaaaa gactatttga atgaaaatga
    25021 ctttccgaca tgggcgaata ttgaagatgc tacatgggat gtagtatgta cgatatattc
    25081 tccttgggta acgattgctg attttgtttt agataaaatt ggaggggata cataccctga
    25141 tgccaaatct cttgctacaa gtgactttaa aacgttctat ggttatctaa taggtctaat
    25201 aggcaatgga atagatttga aagttacgcc aaaacaaaca cctgaagaaa ttgaaaaaga
    25261 aaaaggagag gttcaaatgg cttatgcaaa tgacagagaa aaagtaaaat tattggaagg
    25321 taaattatct gaagaagaac ttggaaaaat ggtgcaagaa atcaatgaag acagcaacta
    25381 ttttcctgaa ttgaaggtat atcaaaatga cgaagatttc tttaatgaaa tgtttagtaa
    25441 tagtccatgg gaagcagtga gtaaaacaaa tttaggagaa tggaactttc aagatgatct
    25501 agttgcattt agcaatgatg ggtatttacg atcatattca gaaagtgagt atagagctga
    25561 attaaaaacg tatgaaacgg atattctgat gagctattta aatctattag agagtggtca
    25621 gggagagtta agagataaag ttgaagaact attaaaagaa gagctatagg gattatttca
    25681 acataaacac aagcagaaaa ctaccaaaca aaacgtacca tgcaaaaagt cggttaaacc
    25741 ggctttttgc tctattatga gaggagatca aaccatgagt gcaacaaccg atatggcttt
    25801 agaagcagta ataaaagata atacaggaaa tgtcgaaaat gtagaagact ttagcgatat
    25861 tgcaatcaat ctttcagcat ggatttcagc tgtaggagaa gaaaatatcg aatttgagat
    25921 caagaatatg gatgacttaa aagcaaaatt tccttttatc gcatcgaaac taagtcaagt
    25981 tgccgaggat aaatcgtgga atgaattagt tagtttttcc gaaaaaattt atgatattac
    26041 gcaagaagat gagtgtgttt catgggaaat gaataatcaa tacaagaaaa ttgacgcttt
    26101 cgtcaaatca cgtatagaag gaacagcttt tgagtttgaa ggtatagata ccgcagataa
    26161 ttggttaaat aaagaatcct ttattaaaag tatagcggat gatcttgaaa aacaatggtc
    26221 taaggaagac tatgcagagt acctaaaaac agaaagagat acagaggtga aagaaaaact
    26281 cactgaggta tgtttggatg aacatattga tcccaatatc gtaaaacaag aagactatga
    26341 agtggttatt gataaagttc ctactgattt tgaggaagtt gtaaagtata tagttgaaga
    26401 agcattatcg caatactctt cagatgagga agtggaccaa gaaactgttc gtaccagagt
    26461 ttggcaactg gtttttgatg acaaagatta taaatatttg agtgatcaaa actcggttaa
    26521 ttggagtgta cgattattaa ttgatcaaaa tgatattgaa taaaatagag ctgaaaagct
    26581 ctattttttt tgataaaaaa ctagctattt tattaaaaat aatttggttc aaaatttatt
    26641 tttttaactt gataattttt tgccatcctt ttttaaattg gctatatatc aaggtttata
    26701 gtgggaataa ataaaaatac atttgacaaa atttacaata tagtttatta tatatttatc
    26761 ttattatgat aacacaataa aaggaatggt ggtatgaaaa agaagaagtt tagtcgatcg
    26821 ttgttaggaa ttttggtttt agttgcggtt ggtcttagtg gcaagggtta ctttcagaat
    26881 aacagcactc aggaacatga ttttcagatc aaaagtagtg ttcagatagg taataaagca
    26941 gacagtgaat caaaagaatt tttagggata cgttatgatc caaacaaata tccaaataat
    27001 atcgtaaaca ttaatgatgg gaaatataca ctgacaaaag aagaccatga agcattatca
    27061 gaaaaaggca gtcaaacctt ttgggtaaat tatgcagatt tagatcaact tggaagagca
    27121 ggagatgttg aagcgttgat cacctatgat gctgtaaatt ctcactcatc taaaaatgtg
    27181 gaacgtccat ctttcaaaag ttctgtccat attgcaggag aatacaagga tggagtattt
    27241 gatcaagaga gagatacttg ggtcggcaaa gattcaaaca atgaaatcac tcagctagaa
    27301 ggctataaag gttatctcta taacaaaagt cattcattag catggtcgct aggaggagat
    27361 atggaggtac acaatttaac acttggaacg agagcgcaaa atgttgggaa aaatgatcag
    27421 aatggaggga tggcttactc agaaacgatt gtacgtgatg caatctataa aaataagaat
    27481 gaaaaagtat tgtataaagt tgatccaatt tataaaggga atgaattagt tcctcgtggt
    27541 tctcatgtcg aagctttctc agttaacgat aatggaaaaa cggtaaacct caatgtttat
    27601 gtatttaaca atcaaaaagg aatcaacatc aactatcaaa atggtacttg ggtaagagat
    27661 tagaaattga gaaggtgaag aattggaaac aataagcatc aatggattag ataaatcagt
    27721 tgcaaatcga ataaaaaaag gaaatggcta ctatcttctg aagaagggta aatattcagt
    27781 tgctagagag ttagaaacaa tgagtatcgt gtatgtagct aagaatcatt ttgacgtaga
    27841 acaggctatt tttgatccag attctgaaat agggaaatgt gttgaattat cagagaaaac
    27901 tgttagtaca aaaggggcga tgatcattga gagagaaatg ttagacgaaa tcagagtaat
    27961 cattcctaaa gatacgcaga tgtgtgagga cagcataagt gacgatgaac caaaatcaga
    28021 aagaaaataa ggagatattc agatgagcga aaaatccatt gaattaaagg aatataagcg
    28081 aagcgatgga gaaaaatatt atcctgtagc cagtttttct aaatcagtat atctggttgg
    28141 acgtgcattg gtagttggtg caattggcat atatattttg cattgggtaa caaaggttgt
    28201 acaactgctt gtagatacct tagtaagtat tcttgtctcg ttaaatgatg ttaacggaac
    28261 agatgattta ggattaaaag agatgtttaa agagttactc aatgcgtttt ttcccaatta
    28321 tttaagttat tcatataatg tacaaagctt ttttgcaaag ctacacttgg tagacatctc
    28381 tattttagta ttagaagcag gaacggtatt ctttgttgga tatttggtta ctttgatgtt
    28441 caggcatcac tatggagaac aaagaccatt ttttgatgat atggaaagta gaaggttgaa
    28501 aagagaagct ctagaagcta tggatgtgaa gtatgatcca aacaagatta taggagatga
    28561 aacgaacaac aaaaaaagaa aacgaaacaa aacgcaatta gaatccgatg tgctatcaac
    28621 gcttaaaaat atcaaaattt ctgttcatac aagacgtgat ctaagtgtca atgaagatat
    28681 tcgagaatat atcgtaaaaa tcccgcaacc aaaagggaag aaagttagag aaacagtttt
    28741 acgacaaatt caagatttag ggtcgatcct gacaagagca acaagcggac gagccaaatt
    28801 tggtgaaaaa gaagatgaag tttcaggcga ttatttcatt ttccgagcat cagtaaatat
    28861 tgaaagagaa cgtaaggaaa gagtaagtac caaaaacaaa gaaacggaaa gagcaacatc
    28921 ttcaatcgca gtttcaaatt cacaattttc ttttccaatc aatctctttg ccgataattc
    28981 caaaaatatc caaaaccaaa ccgaaaaagc tagaaaatat gccgaaactc aaaagcaatc
    29041 tttagaactt tatctatctt ctgctgacgt acctgcaagt ctaacagata tgcacgttgg
    29101 gaatacatct attgaatatt tgtttacttt atctcgtacc agaaaaattc aactaagctc
    29161 aatgaaagaa gaaattgaaa cttacttgca tatatcaggt gtaatcgtgc ggatcaacgc
    29221 agggcgtatt caaattactt taccattagg agaaaatgat cgtattccaa ttgatgtacc
    29281 atcaatgatc aatagcgtgt tttagaagac atcacaaata gaaacagaaa atacaaacta
    29341 ccagttcaca aacagaaaag taccgagaaa agtgaggaat agaaatggct atgaaattat
    29401 tagagaaagt caaaaagaga gccgaaaaag ctgaatctat tttgctagat aaagaaccta
    29461 atataaagct taatggaaaa ttcagtgtca atatgttgaa cacaaaggct aaagacccaa
    29521 cagatgctat ttttggaatt aagcctgatg gcacaattgc tcatttttgc atcagcgaaa
    29581 tgccacacat gttggttgca ggaacgacag gatcagggaa atcagtatta ctgaatgaaa
    29641 ttcttgttac agtaatgtgt cactcaacgc ctgatgaatt aaaaatagga attgtagacc
    29701 caaaaagggt tgaatttgga agatacaaaa aattgccgtt catgttggct gatccaatta
    29761 cagatatgga tgaagcttat gatttttttg aatatcttgt gatcttgatg catgaacgtt
    29821 acaaactgat ggaaaaagca gggttacaaa acatcacgct atataacgaa tatgccgaaa
    29881 aaaatggatt agatagattt ccctatgtga tcttattagt tgatgaatat tcacagttgg
    29941 ttggaacaca taaagaagta gaaggtttga ttgttcaatt aggacaaatg gcaagggcag
    30001 caggtattca tgttatctta gcaacacaat ctcctcgatc aacagttgtc actggtatta
    30061 tcaaagccaa ttttccgtca cgtgtttgcc ttatggtagc aagtgattta gaaagtagga
    30121 ttgtattaga tgaaggcggt ggagaaagtc taagtccaaa gggagatatg cttattaagc
    30181 ttgttgatgg aagtatggtt agagcgcagg gagcttacat cagtaacaaa gaaattgaaa
    30241 caatcttcaa tcaccttaga aatactatgc cagaaccaga atatgttgat taccgagcaa
    30301 tcgtagctga aactcgtggt gagggcggtg aagacgagga ttttggtgga attggtaaag
    30361 ctcctgtaag aaaatctgct aaaagaaatt tgaacacacc tttagcaaat ccatcatcta
    30421 aagagccatc aattgaaaag aagagtgcgt ttgctattaa gcttgaaaag aacaaagaag
    30481 aaaaagtcga gcagggagct aaaacactag aaaaactgca agcagaaaaa ctagaaaaag
    30541 ttttaagggc attaggaaaa gctgatcctg aaccgcaaac ctcaaacaac acgaataata
    30601 aacagccgat tgcatctaaa actaaaccta aagctaatga tccaaaaggc tcagaaaacg
    30661 tagtgatcac aactgataaa tctgaaaaga aacaaataaa tggaagtact tcacctatca
    30721 tgcaaaaaat gttaaaagca aaaaaacagg tgcataaatg atcatcgtca gttctttttt
    30781 aagatggctt actccatata aatatacaga tgaagtagtt gtgaaacgag cgaagaaaag
    30841 agataaaaaa gcggtactaa ctcctaaaca gatgcttgat catggcattt taggggtgaa
    30901 ggttttagaa gtgaataaac cgtttgaatc aaatagtgca agagaaaagt tgcttggcgc
    30961 tgaagccgat gtcgcatatt tttaccatga caaaagcaag catcaaatag ctattaaatt
    31021 caacccgaaa tcggcatggc ggtatcaagc aagagatgac ttgaaacgta ggaaacttaa
    31081 accaatgatc tatccagaag gaaaagaagc atttgataga acccacatcg ttcccatcgg
    31141 ttttcatggt acagaatcag ataatcgcct attagtaggt tggaacagca atcaaaatag
    31201 aattcagatc aataaatttg agaagaaagt aagaaaattc aacgatctaa aaacaatctt
    31261 atggtttgca gatatttgta aacagccgga cggaacagca aagtgggaag cggtgatatt
    31321 tgacgaaaat ggtaaaaaag taatgtctga aacatggcat gataaagcta aatttgtttg
    31381 gcagtagcag tagaggtgaa aaagattgct agaacttttt gaagtaaata gaacaggctt
    31441 taatcaaaaa ttagaagtgg gagaaactat agaatataaa gataaaaact atattattat
    31501 cggaatctat aacacgagga ttaacagaat aggtgatcca cgtataacca gtgacttggt
    31561 ttgtcaatct gttaactatt cacctgatct gacaagcagg cataagaaat atgtgttatt
    31621 agaatataga cacaagatta ctgatgaaat tggagtaaat aaaacacgaa atatatttaa
    31681 gatcggaaca gtgattcctt atagtaacaa cgaagaaaaa atattttatc agatttacgg
    31741 aatagaaaag tttgaatatg aacatgtcga tctgctagtg acgtaccaaa tgcgtttaat
    31801 agaaccttgg tcacaggcag aaattgataa agcagtaaaa ttgaaccgat tatcaaaatt
    31861 taacgtatta ggaaatgcct tttaaaaatc gcaatacaca aacagaaaaa taccaaaaaa
    31921 ttagcaatga acgtaggtaa gaacatgaca ataaataata ataaccctaa aaaatataat
    31981 gctcttgtcc aatgctatat cgaaaacaaa attacaatgc aagaagtcgc tgatgaatta
    32041 ggcaaatcaa aaagcctagt atcacacgac tttaagcaca atgtttttcc gaacccgatt
    32101 gagctgagga aaaaatttca aaagagagtt tatcaagaaa tagggaagtg gattgatgaa
    32161 gggaatgcaa ttgacccttt aattgaaaaa tatccttttt taaaaagcaa aaataaaatt
    32221 caaaggttaa ttcgtcaagg cgatattcaa gctaaagaac ctgttttgac aaccaatgat
    32281 ttggctaaaa taaatcttca taaacagatt attgaagact ttgaaaaaaa tctttcaaca
    32341 actgaaattt taaaaaaata caatattccg agaggaaagc taacatcgac tctttttaga
    32401 gaatatggag aatcggcagt tactaaagca aaggaagtag cgaatgagag aaaaaaacaa
    32461 aaatagtagt cgatacctag tagcgactgt agtttttgtt tgtgtattga ttttcctgtt
    32521 tgattttttg cagattggga aaagcataat tggaaataca ttgagtgcaa aggaaatggt
    32581 tgctttaggt ggtttgtatg aaggacattt gttcccgaat atattcattt ctatcgtttc
    32641 tcatattaac ttgtaccatt taatttctaa tttattgttc ttagtagttc ttgaagaagc
    32701 actgaatgat caatatgagt ggttaacact attgattgtt ttaatcatat caggacttct
    32761 aggaagctta gttccttttc tgttacaaag caacatcgta agtatggggc ttagtggagg
    32821 tatttttgga tggattgctt tatttgcgat tgcatacatc cgctttgata gtaaggaaca
    32881 tttattacta agtgttttta cattattgat aatcggcatt ttactggtat ctacttttga
    32941 atcaggcgag acaaacgtct tgtcacatgt aatcggattt gtctgtggga tgatcttggg
    33001 atttattcta aagaacgttt cattgatcaa gcaaaagaat taaaagggag tgctaaaaat
    33061 gccaaaaaaa aaagaaaaga aaattccagt gtttttgtct gtctcagaaa tgttatcact
    33121 tcaaatactg ttaagaaaaa agattgaaac aatagacgaa gaagttagaa ataaaagggc
    33181 tatgtacgaa caacgtgaag cgctaagaga acgaatccac tattttgatg atatacacac
    33241 taaaatctat gaagcacgta caaataacga agacgaaagc aaattaggat tagctcaaca
    33301 acgaatattt gaatggctaa atacgaatac tgagcaagaa ccgttaagag caattagtaa
    33361 cctttttgaa tttggaacga agcattggtt taacgcagta gaaaaggatg ctgacttaca
    33421 agaagcgttt gacacgctaa gtaaggatag caaatccatg cgagatttat tgattactta
    33481 tctaaatgag catgagtttg aacaaacgaa aaccaaaaag aaaaataaag aatagaaagt
    33541 tgtgatgaat catgctatta accctgttag gttatttatg tttaattctt gctttttttg
    33601 gggcaatcgc tataattatt ggtcaatttc aaacaaataa tccgattatg cgtaacatag
    33661 gtattggaat tgtgattgta acctttattg tgtttaatgc cactaagatt tttggaagta
    33721 acgataaaca atttggtata tcgggagtta ataatagtta tgctccagat tctttgggaa
    33781 gggtagtaat agatggaaca tttgaaaatg taacgagtta taaaaatgtt tcgctgacag
    33841 tgaaggaaga tggcaagaaa gcatcagacg acgttctcgc tactattaag ccgtcacaat
    33901 cacaatttga agtgttttat acagtcaaag agaatagccc tgtacacaag ctgacattgg
    33961 tttttaacgt tgatggagaa gaatactata aagatgtaaa aataaataaa acatcaacga
    34021 aaaagattat cgaatcagcg aaaaaagaag tagctacaga agaaagtaca actgaatcaa
    34081 gttctactgt cgaagcggaa ctaccagata aaattgattt tgatcctgaa aaagtcaaat
    34141 cgtttgaatc tgtttctggt tctatggctg atcggtttaa cactgaagtc ttcaaagcta
    34201 tcaaaactgt taaattggat actgaaaagc aaatgattaa tgttacctta ttcaattcag
    34261 ttgaatctat ggatcaggat aaaaaagtta cggttgcaag agaactagga aaaagagtaa
    34321 ttggtttgta tcgtggtact gctaaaccag attcagacgg tgcagatttg ccagttcatg
    34381 tagtttatga agacggtaaa gtttttggag attctacagc aaacgaagca ttaacgatta
    34441 aattggctaa atagccagaa agttataata aacaaatggt gtcacaccat taatatatag
    34501 gtgtgacacc ttggagatga tagctgatgg tagtaaataa agaaaaagta agaaagtatg
    34561 tgactttaaa aatagcttct gttaaatatt tagaaaagcg agctaaagaa gaagaaagag
    34621 caagaggtaa gaaggtaaca gtctcggagt tgttagaaga attgatcgag aatgatcaaa
    34681 caataagacg tgcatttact aataggtcta tcagataaca atataattaa taaaaaagaa
    34741 agagtgattt ggtcttagac taaatcactc tttttatata aagtttcttt acacttattc
    34801 cgaataatgt taaaattctt tatatgatat atagttactt gtcagagaaa gagataacca
    34861 gcaatattgg agggataata agtggaaaac aataatgata ttagacattt tagcattggg
    34921 gaaagagtta caacagtaag tgaattatca aaggattata gagataaaga gggaacgatt
    34981 atttctctta ataagaatcg aagaggcgtt tggcaatatg ttgttgattt aggtgatggc
    35041 gttacagttg agtgtaaaga tagtcaactg atcagaaatg atggcagtca ttccattgaa
    35101 aaagcaattc ttgtataccc aaaagtatca agtaagatca tgaatgactg gttgcttgga
    35161 aaatttggtg agtacaataa aaaagaaatc gcagaagtga tcggcaaatc ttatccaaca
    35221 gtattagggt atctaaaaaa gataacgaac aaagaagcga aagttgagga tgttttaaca
    35281 gtaagagaac ttcagtcatt aatcgaatat cggaataaac aaataacaaa tcctgagtac
    35341 cttaaaaaag aagatggtct attgaaacaa ttggaacagg taaataaaat ccggaagatg
    35401 caaaaggtag ctcctttaag cctagataac tttaaaaaaa tttatttgaa agagatcgaa
    35461 aaagactaat gtggagtgtc actttaccgt ttcaacctgt atctgcacca agaccaagtg
    35521 caagaaggaa tgtttctgat gcaactaatg acgtagcaga gaaaaggatt tcaacctatt
    35581 atgataaaaa gtatctcgat tatttgaaaa aaataaaaag ctttatcgaa gatcaaggtt
    35641 tgttagatga tgaattttac tcgatcgtaa atgatccgat gggtgcaatt atggaagtgg
    35701 acttttacta tcagatacca aaaagctata aaaaagtcta taacattatg aaaacgacag
    35761 cgccagattt agataattta gtaaaaagtg ctatggatgg tattttcaac atctcagcta
    35821 tacgagatag ccatatcaca ggcttaattg cttttaaata taatgtggcg aacgaaggaa
    35881 aaacagttgt gaggatcaaa cgatattcag aatttgagtg ggatacttct gaaggattga
    35941 acggagatat atgggaagct acatttgatt tcaaaccagt agcaacgcca agaccaaagt
    36001 ctaaatttag aggaaacggg attatcactt actatccaaa agagtacaac gactatttgg
    36061 aaaatatcaa gaatacgctt aaagagaaag atttggtaaa tgatcaactg aaaaaggtaa
    36121 tgaatgctcc tatgggggtt atagctcaga tagattattt ttaccaagtg agaaaagata
    36181 agaaaaagct tgattcactg atgcgtactt cacagccgga tattgataat ttagtcaaag
    36241 ggacattgga cggaattttt aacaaggaaa taggcatcaa agatagcagg tttgtagggt
    36301 taattgcttt taaatacaat acttttgaaa agtcgaaaac aaacgtgaga ctacaggaga
    36361 tgggctgatg aataataatg aaaaaattct tcatgtactt gatagctttg aaacaattca
    36421 agaagaacta aagaaatatc gggatgtttt ggaacaaaga tatgattttg tcaatcagca
    36481 aaagagtaat cacatggatt tcattctcaa tatgaatgac ttgaagaaga aattaggtga
    36541 acgaaaagaa caagaaaagt tgatcaaagc ttactttgaa ctcggtgaaa aagaagttaa
    36601 aaaggcaatg gaactgaatg aagatcgcag ggttttagat cagctgttag aacaattatt
    36661 ggtgatgttt caaaaaggac gaatagatga ggatttaatt gaagaagggg tgagaaaata
    36721 tccttcaaac tcaggtatcg gaatagttct gaaagcgatt gatgaagaag aaattgaagc
    36781 tttcataccg ccagaggatt ttgaatcggc aatggaatat ataaaatatt atagccaagg
    36841 aatcactgct tttagagagt ttgatcctga agatgttatt catgacttga acaacctaaa
    36901 agaatggtgt gaaagttatg aagtcgatga ttctggattg gactacttga tcagtattat
    36961 ggaaatcgaa gaagagatgc cggataagcc tgatccaaca gatattttag aattgatcca
    37021 tgaagctaga aatccaattg cctatatttc tagaggatat actgtattgg aatactataa
    37081 gccttatata tcagcgatga accatttaag acgtgtatta agggaaaaac gtgaataccg
    37141 ctcagtgttg aatgcaatga atagattaga aaaagctgtt tcagagctag atgcttatta
    37201 tcgtgaacat tacctgcaag caggtggtat gccaagaaat acgaaagcaa atatcagtag
    37261 gtatataaaa aaagctgagt aggcggagtt agcccataaa atggcaaaag gtaacctgaa
    37321 aggggcattt tgaatgaaaa gggatgtttt tgaattttac gtattagaac gtctagaaga
    37381 cggcatgatg gcacaagaga taattgatga aattgatgat atgatcagca gatttggtca
    37441 attggagttg aatgatggaa acaaaaattc ggtagtgcta aaacaatttt ggttgaataa
    37501 tttttcgtat aaggatttta tcaaagaaaa ggcaaataca ttgtactgac taaattcacg
    37561 aggatcagtt atgagagctt attgctaaga gcagttaggc tcttttttta tttgtgtaaa
    37621 attattttct tcgcaataca catttataca caaaaaatat tgacgaatag tacacaaatg
    37681 tggtaggata catttgtaag caagttagat taacaagtta aaggaaggaa gagcagttat
    37741 gaaaaaagaa gaactaatag atatatttta aattgttgaa cgagcaaata atatgggaat
    37801 tatgtttttt gatcgtattt ctttaaagat ggatttaagc attgcccacc aagaatttaa
    37861 tcttcgatta aaagccttgt tgaattctga tgatgttaat ttcgctcatg atgttgtagg
    37921 tatacagaac catatagata gagaaagcaa acgtatgggt gatgaattct taccaagata
    37981 ttcaagttta tgatttggcg aaagattcaa gagtatgtgt cattattaat aggtattctt
    38041 gaattggaaa ggaggaatga atttaagaca acaacgatta tgatcttaaa acaatcgttt
    38101 atgtaattgg caatcttacg aataattttc atgcaataat gacaataaaa aatgaaaagg
    38161 ggtaaaatca aatgaaaaaa attttagtag gaattctatt cacaatgggg ctagtgttat
    38221 tatcaccttc agtttcagca gaaagctcta tttccatgta tcgtatgtac aatcccaata
    38281 gcggagagca tctatacact agaagcgttg gagaaagaga caatctgaaa cgttatggat
    38341 ggcattacga aggtgtcgca tggcaagctc caacaagtgg tactcctgtt tatcgtttgt
    38401 ataatcggta taacggtgaa catttttaca ccgttgatgc cggagaaaga gataagattt
    38461 cacacacagg atggacgtac gaaggtgttg cgttctattc ctacaacgga gcaaatagag
    38521 ttcctgtaac acgtttatat aacaaatacg tgaattggca tcactacacg ttagatcaaa
    38581 atgaaaagaa tgtaattagt aaacaaggtt ggaagattga aggtacaggc tggtatgcaa
    38641 tgccaaacag cgtaccaaat cctgcacctg ctccaagcac tgaaaaagtg ttgaacgttc
    38701 ctgttgtatg gcaaggaaat acaatgttat gcgaaggagc ttctttatta gaaggtttac
    38761 actacaaggg tgtagccaca aatcaaagct taaccagttt tgtaaactat atgccaaaag
    38821 ctaatgacaa taatccttat catggttact caggagagtg gcgtcataac gttaatggaa
    38881 cgtatcaagg catgatggct gacccagtag ttaaatgggc aaaaaacgtt ggcggtaatg
    38941 ccgtcaatat cactggttca ggaacgaatg gaatcaagaa tgaaattcgc aatggtaatc
    39001 cggttgtaac ttggattact tataactatg ctacgccaga gttcaaacaa atgccttggg
    39061 gtcgtgcagt atggaatgga catgtagttc tcgtggatgg cttcagaaat ggtgcatacc
    39121 atgttgttga ccctgtattt ggcgcaaaat ggatcaatgc aagcacgtat gagagagcat
    39181 ttaatgtgac tggaatggcg gttgctgtac gttaattgat agaattatca actaaatgaa
    39241 ataagagctt aattactgaa atggtaaata ggctcttatt ttttagaggg aatttttgta
    39301 atacgaatct ataacaaaaa atattgaatg tagtacacaa aagtggtatg ttaaaatcgt
    39361 agagattatg cgaagaggtg attttgatga accaaagaag aaaaaggcta gaagaaataa
    39421 gcgagcgact ttccgagtta aaggatgagt tagaagtaat tcaagaagaa gaggaagagt
    39481 acaaagaaaa tatacctgaa aatctacagg gatcaaaaag atatgaaaaa gcagaagaat
    39541 ccgttgacca acttgaagaa gctgttgatg gtttatccga catattagga ttgattgaag
    39601 atataactag ctagagatga ttaatgaaga tttcaggagt gagcaaaaaa tgattgatat
    39661 ttcactgata gagaagctac ttttctcaga taaatacacc gctaaagaac tggctacaag
    39721 atggcaaata tccgaatcag caattaaagc ctatcgtcaa aataagggaa gcgtaggatg
    39781 gcgtgattgg aaagggatca gcattggaaa agcagaagac ataatgaaac gtgaaggact
    39841 tcaatcctat accacttctg aaaaagacaa ataacttaat gtttgaaaag ataaattatc
    39901 tgtcttaaag ctatgtcaaa ttcaaatggt gaatgtttgt taaatatatt gcaaaaaaag
    39961 gaagagaaat attaaatgaa aagcgaaaat ttgtcttatg agaataataa acatctagtt
    40021 aaagaaaata agaaagtaga agattataag aacatgtata tagaaatgaa aaaatcacca
    40081 ctgtgtattc aatataaaaa tgataattgg aagcaagtag tcgactttgt aaaacctcat
    40141 agtatacgaa gagtacaaag gggaagaaaa aaagtgaact tgtaaatagc ggaagagcgg
    40201 taattagagt tataacgaaa gattatccag ttgaatatgg agattatatt ttaaggttaa
    40261 gttatcatgc ttttgttgtg ttgaaagaag aaaatttcaa cgagtttttc aatataaaaa
    40321 gtaagtaatc atagaatttt ttctggcgtg tataaattta attaaggagt aacaagaaat
    40381 ggaatttgat gatcttaaaa aaatcttact gaaaaagaac tcagcgagtt tatagaagat
    40441 ttcgagaaat acaaagagac aagaaaaaaa ctgttgcagc aaagaaaaaa agatagagta
    40501 aacaaatctg atttgtattc gtcaatacaa aaagcattag aaaatattac taaagactaa
    40561 aattaaattt ttatatttga cggttgacta ttttgattaa ggaataacag ctaaacagcg
    40621 ttcatgattg tgaggtgatg acatggtagc aaaaaagaat actctaaaag aagcttctga
    40681 aaaatatcaa gatatgaaaa gacgaggatt aataccaaag gacaaaaaag gtatatggag
    40741 agaatttgtt aaggaagtca gtagtgatcg aagtggcggt gactttgttg gacgatatac
    40801 agcaaaaggt tatagcaggc gcttaaggat cactgttgaa tatggaacga atgaatcagc
    40861 tacagagctg aagcaaaggt acttagatac acgaagagaa gcttcaaaaa aatatgatga
    40921 tttcaagaga agaggtctaa ttcctaagaa gaaaaaggga atttggcgtg agtttttcaa
    40981 agaagaagat agcaatcgga ataatggaga cttcattggg cgatatacag caaaaggcta
    41041 cactaagaat ttagggatca aggttgaata cggaactggt gaaagttcat gggaattgag
    41101 gaaccgctat ttaaacatat tgttagagaa atacaaagca gagttattgg agttgttcga
    41161 tgttaatgat tttatcgaga tggctgaaaa gtatggtcat gaagaaggtt ttatgatcaa
    41221 tgacattatg gttattaata attttacgga tttattagcc tttgcgaaaa aagcaaggga
    41281 tttaagagaa acgcagatgt atctattcag aaataaaaac ggaagaaacc ggagaactga
    41341 atcgagtgat atcgaagatt actacgatgc ctttacacac gatctaggag cctttgtaga
    41401 cgagataagt ggcgtaatgg acaaatatag ggtttagaat aaaaaagccg gagatgagca
    41461 gataaatggg aaacaatagc atatacacac ctgagatgca gaataaaatt aaaagacttg
    41521 tcggattagg gtggatgaca agaggaaaat tagattcttt tagtacaatc aaatatcttt
    41581 tgatggacat ttaccgtcag aacaagcaaa caggaataca ttatttaggc tatgataagc
    41641 cggaagatgc tcctttcttt gttcctagtt cagcctatag agccagtacc tccggtagca
    41701 tgtataactt ggatttgttc gaggaaggag caaagatgat agagtatgat ttcaacgaag
    41761 cctattcgaa gatcatgaga aattatcctt tgccgtcaaa cacttatttg aaagcaaaat
    41821 ataatacaga tcaagctatt ttgagagcga taaatagacc aaaagtaaga ccgtatgaaa
    41881 atttaagaac atatatgttc tttttggtta attttgaagg cacagctaaa gaaggaactt
    41941 atgcaaaccc aaaatcacag tcgatgtttg cggattatgg cgaaaatccc aaaacagcta
    42001 atgaacctat ttggctatca gagattgagc taacgcttat ccttgattac tacgatatta
    42061 aagagatgga agtcttagat agtcacacat ttttgacgaa aaaaggatta ctcaaagaat
    42121 attttgaacg aatagagccg ttaaaaactg atcctgatac gttgaaacta tataaaaatc
    42181 ttagaacaaa agtatttgga gctattggac aaaaaacgtt aagagaagga gaagtaaaca
    42241 cttttgataa atacagcatg tataatcgtg cgtactcctc gatggtagca ggcgttttta
    42301 gagatcgcat ggttaggtat gaacagaaat acgttcacag cgaatatggg ctattaaaaa
    42361 tccgtactga tggattgtat ttcagatcag aggtgccaga gtttgaagag ctatataaac
    42421 ttggagtagt aaagaaaaaa gttcatacaa tatccaaaga agaagtcgaa aaagccttag
    42481 aattcaaaaa taaaggattt gaaaataagg gttcccttgg gaagtctaaa gatgaagtta
    42541 agggtgttgt gattgatgat gatttcgagt ttgattttga ctaaatactc tttggaaaag
    42601 atggtaggag gtaaatatga agaagaaaat tgtggaagga aatgtaggtg ttattattct
    42661 aaaagattca actgactatg ctaaatcaat ggcactaaaa gacgaaaaag ttgcacgcat
    42721 ggataaagat ttagttgaat ttctgatgga tgatacagaa agattaaaag gggatcagtt
    42781 tttgagttta atgaaagaca aaggcttaaa cacatatggt ttatttgatt ataagaaatt
    42841 agctatagag tgggtaaaag aaggagaaat attggatgtt tcaatgattc ctagtgaaac
    42901 ctagttaaaa ttatcggatt tttgagattt acaagaactt ctgatgtatt tttgtactac
    42961 ttaaaagaga gggtcaaaaa aatggtcaag gacaaaaaaa agaaaacatc ctatgctcag
    43021 gatactctct aaaaactgat aaatcagcgc ttctcttatt ccgcagcgtt tgcgtctttg
    43081 agaccgtatt ttttattgaa gaatgggaag gtactgagaa cttagttctt catcagtatc
    43141 ttttgaaagc ttaatctaag taaaacagtt ctgacaagtt atgcttgtca gaatttcaaa
    43201 ggatgttttc cataaccatc atcattcaaa aataaatttt caaaataaat taactgaact
    43261 attacccaat attaaaatgg ttgatgtaag cgaaaacaat ggaaagatat ttaaaaattt
    43321 gaatatgtgt gatgcgattt ttctatatac agattacttg aatcatggtc tatattatcg
    43381 tgttatgagt gaaatttcta acaaagacaa gatattttat ttggacaatc atgtgaatat
    43441 gaacttaaca attaaacgta tttacgatct gttatcactt atataaagag atatgtgata
    43501 aataggacac aaaatttggg aagtaggtaa gtaactatta gtcagacttt tagtctgact
    43561 ttttatattt tcggagattc ccctttacaa aaaacgaaca tacgtttgca taattgcttg
    43621 tgaatggggg aataaaaatg tttgattaca gcagagaacc ggtgaatgac tattttttaa
    43681 tcgacatgaa gtctttcta
//
LOCUS       DACEPW010000003        42645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEPW010000003 DACEPW010000000
VERSION     DACEPW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.003"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..725)
                     /gene="dusB"
                     /locus_tag="IW819_000112"
     CDS             complement(<1..725)
                     /gene="dusB"
                     /locus_tag="IW819_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1243250.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRT"
     gene            complement(726..1613)
                     /gene="hslO"
                     /locus_tag="IW819_000113"
     CDS             complement(726..1613)
                     /gene="hslO"
                     /locus_tag="IW819_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1243251.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(1830..3941)
                     /gene="hflB"
                     /locus_tag="IW819_000114"
     CDS             complement(1830..3941)
                     /gene="hflB"
                     /locus_tag="IW819_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1243252.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(4031..4576)
                     /gene="hpt"
                     /locus_tag="IW819_000115"
     CDS             complement(4031..4576)
                     /gene="hpt"
                     /locus_tag="IW819_000115"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1243253.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(4576..6021)
                     /gene="tilS"
                     /locus_tag="IW819_000116"
     CDS             complement(4576..6021)
                     /gene="tilS"
                     /locus_tag="IW819_000116"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1243254.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(6141..6611)
                     /locus_tag="IW819_000117"
     CDS             complement(6141..6611)
                     /locus_tag="IW819_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1243255.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(6657..7082)
                     /locus_tag="IW819_000118"
     CDS             complement(6657..7082)
                     /locus_tag="IW819_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1243256.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(7149..7418)
                     /locus_tag="IW819_000119"
     CDS             complement(7149..7418)
                     /locus_tag="IW819_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1243257.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(7429..9024)
                     /locus_tag="IW819_000120"
     CDS             complement(7429..9024)
                     /locus_tag="IW819_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1243258.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(9039..12560)
                     /gene="mfd"
                     /locus_tag="IW819_000121"
     CDS             complement(9039..12560)
                     /gene="mfd"
                     /locus_tag="IW819_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1243259.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(12577..13137)
                     /locus_tag="IW819_000122"
     CDS             complement(12577..13137)
                     /locus_tag="IW819_000122"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1243260.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            13490..14464
                     /locus_tag="IW819_000123"
     CDS             13490..14464
                     /locus_tag="IW819_000123"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1243261.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            14634..16034
                     /locus_tag="IW819_000124"
     CDS             14634..16034
                     /locus_tag="IW819_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1243262.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(16112..16834)
                     /locus_tag="IW819_000125"
     CDS             complement(16112..16834)
                     /locus_tag="IW819_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1243263.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(16913..17419)
                     /locus_tag="IW819_000126"
     CDS             complement(16913..17419)
                     /locus_tag="IW819_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1243264.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            17542..19341
                     /locus_tag="IW819_000127"
     CDS             17542..19341
                     /locus_tag="IW819_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1243265.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(19409..20983)
                     /locus_tag="IW819_000128"
     CDS             complement(19409..20983)
                     /locus_tag="IW819_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243266.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(21238..21747)
                     /gene="mreD"
                     /locus_tag="IW819_000129"
     CDS             complement(21238..21747)
                     /gene="mreD"
                     /locus_tag="IW819_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1243267.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(21751..22605)
                     /gene="mreC"
                     /locus_tag="IW819_000130"
     CDS             complement(21751..22605)
                     /gene="mreC"
                     /locus_tag="IW819_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1243268.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(22760..23398)
                     /locus_tag="IW819_000131"
     CDS             complement(22760..23398)
                     /locus_tag="IW819_000131"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1243269.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(23399..24049)
                     /locus_tag="IW819_000132"
     CDS             complement(23399..24049)
                     /locus_tag="IW819_000132"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1243270.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(24062..24961)
                     /gene="rsgA"
                     /locus_tag="IW819_000133"
     CDS             complement(24062..24961)
                     /gene="rsgA"
                     /locus_tag="IW819_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1243271.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(25126..27195)
                     /gene="pknB"
                     /locus_tag="IW819_000134"
     CDS             complement(25126..27195)
                     /gene="pknB"
                     /locus_tag="IW819_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1243272.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(27192..27932)
                     /locus_tag="IW819_000135"
     CDS             complement(27192..27932)
                     /locus_tag="IW819_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1243273.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(27945..29303)
                     /gene="rsmB"
                     /locus_tag="IW819_000136"
     CDS             complement(27945..29303)
                     /gene="rsmB"
                     /locus_tag="IW819_000136"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1243274.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(29303..30241)
                     /locus_tag="IW819_000137"
     CDS             complement(29303..30241)
                     /locus_tag="IW819_000137"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1243275.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(30234..30725)
                     /gene="def"
                     /locus_tag="IW819_000138"
     CDS             complement(30234..30725)
                     /gene="def"
                     /locus_tag="IW819_000138"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1243276.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(30740..33148)
                     /gene="priA"
                     /locus_tag="IW819_000139"
     CDS             complement(30740..33148)
                     /gene="priA"
                     /locus_tag="IW819_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1243277.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(33401..33706)
                     /locus_tag="IW819_000140"
     CDS             complement(33401..33706)
                     /locus_tag="IW819_000140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1243278.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(33711..34325)
                     /gene="gmk"
                     /locus_tag="IW819_000141"
     CDS             complement(33711..34325)
                     /gene="gmk"
                     /locus_tag="IW819_000141"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1243279.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(34812..35096)
                     /locus_tag="IW819_000142"
     CDS             complement(34812..35096)
                     /locus_tag="IW819_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243280.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(35114..36004)
                     /locus_tag="IW819_000143"
     CDS             complement(35114..36004)
                     /locus_tag="IW819_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1243281.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(36024..36242)
                     /locus_tag="IW819_000144"
     CDS             complement(36024..36242)
                     /locus_tag="IW819_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243282.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(36212..37912)
                     /locus_tag="IW819_000145"
     CDS             complement(36212..37912)
                     /locus_tag="IW819_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1243283.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            38179..38952
                     /locus_tag="IW819_000146"
     CDS             38179..38952
                     /locus_tag="IW819_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243284.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(39033..39602)
                     /gene="thiT"
                     /locus_tag="IW819_000147"
     CDS             complement(39033..39602)
                     /gene="thiT"
                     /locus_tag="IW819_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1243285.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(39668..39770)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(39776..40747)
                     /locus_tag="IW819_000148"
     CDS             complement(39776..40747)
                     /locus_tag="IW819_000148"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1243286.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(40841..42214)
                     /gene="glmU"
                     /locus_tag="IW819_000149"
     CDS             complement(40841..42214)
                     /gene="glmU"
                     /locus_tag="IW819_000149"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1243287.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
BASE COUNT        11646 a         9254 c         7735 g        14010 t
ORIGIN      
        1 gtgcggtgaa tcatccatgg attgcccagc gctgcacgac caatcatcac accgtctgca
       61 ccgacatagt caagcatcca ttttgcatct tctggcgttt tgacatcccc gttccccata
      121 aatggaatcg tcaaatgttt tttcacttct ttcaatactt cccagtccgc ttttccttca
      181 tacatctgaa cccgtgtacg tccatgcata gcgatggcac ttgctccagc tgattgtgct
      241 gctaatgcat tttctacagc aaagacgtgc tcttcatccc aaccaatacg catcttgatt
      301 gttactggga tatttactgc tgaagaaaca gcttggacca tttcgtatac tttctcagga
      361 tctaaaagcc attttgctcc tgcttctgct ttgattactt tatttactgg acagcccata
      421 ttgatatcta ttatatcggc agctgtgttt tcttcgacaa attgagcagc ttcaactaga
      481 ctatctttat ttccgccaaa tatctgtaaa cttaatggat gttctgtttc ttcaatgtgg
      541 agcatctcta aagttttttt gtttctgaaa tgaatacctt ggtcactgat catttcacat
      601 acgactaagc ccgcaccaaa ttctttgaca gtgactcgaa atgctgaatt tgttattcca
      661 gccatcggtg caacaacgac acgattaggg atttccacat tgccaatttt ccatgtgtgt
      721 tccacttaaa tccctcctaa ggcttctttc ttcaatccat ctaattcttc ttcgctatac
      781 ttgtatttgt tattacagaa agaacaaacc gcttctgcac cgtgatcttc gtctatcatt
      841 gcttggattt cttctgcacc taacgcgata attgcttctg caaatttttc ttttgaacag
      901 tcacatttga attgaacagg cattttttcc aaaatgttca attcttctgt accaagcaat
      961 cctttcaata tttcttctgg tgtctgtcct tcatctaaga gggtagagat tcttggtgtc
     1021 gtttttaatt gttcttcgat cttgctgatt gtttcttcat cggcacctgg catgatttgg
     1081 atcatgaatc ctcccgccgt cttgatgctg tcgtcagtat tgaccaatac actcagtcca
     1141 acagctgaag ggatttgttc tgagatagca agatagtaag taaaatcttc agcgatttct
     1201 ccagaaacaa tcggcgtttg cccagaaaaa ggttctttta atcctaagtc ttttgtgaca
     1261 gtaaacattc cttgtgttcc aactgctccc cgcacatcga ttttaccttc actatttaga
     1321 ggaagactga gatgcggatt tttgatgtaa cctttcacat ctccctttcc atttgcatct
     1381 actacaattc ctgttgctgg accatcgcct tggattcgaa cagttagttt gtcttctcct
     1441 ttgagcgtcg caccaagtag cagactaccg accaatgtcc gtcccaaagc agctgaggag
     1501 gtgtgccaag tatcatgacg tctttgtgcc tccgctacgg tatttgttgc ttgaacggcg
     1561 tatgctcgga cgaatccgtc gtgagcaagt gccttaacca aataatcttt catttgttat
     1621 gtactccttt ttccgttttt aaactcatag tgaatcatac tagcaactac tgcttttttc
     1681 aactttggaa tgcttctttt aaaaataatt cagaatcatc tagaaaacag gacatcaccc
     1741 tatttacaac ttaaataaag aaatctcccg aaaaccggtt tttgttccgg ttttcgggag
     1801 tcatatcaga tcaattgacc tatatttttt tatttgtatt gatcgtctga atgattatct
     1861 tctgattgtt gagaatctga atcatgttct gctttatttt cctcattcag ttcatgtttt
     1921 gcttcttcaa aatcacgttt ttcttccgct tgtttttcag cgtctttttc ttccaaagca
     1981 cgttttgctt cttcaaaagt tttcgcttct ttttcgcttg gataatcatt tgactcagta
     2041 ccttcaggca tcacaccgtg ttcaaacaat gacttgattg ctttggcatc caatgtttcg
     2101 aattccaaaa gtttttcagc aatcagttta tgctgtgcac gatgagcttc gatgatttct
     2161 cgagctttat catgagcttc catcaagatt tttcgtactt cttgatcaat ctcgaaagcg
     2221 acttgttccg agtaagcttt tgtttgacca tagtcgcggc caacaaacac ttgatggttt
     2281 ccttcatatt gaacaggacc taaacgatca ctcattccat attctgttac catgcttcgt
     2341 gctagagctg tcgcttgctc aaagtcattg gaagcaccag ttgattggac attgaagatg
     2401 atttcttcag ctgtacgtcc accaagtaag cctacgattt gttcaaacat atcttctttc
     2461 gtcatcaaga actgatcttc ttttggtaaa gcgatcatat atccgccggc gcgtccgcga
     2521 gggatgatcg ttactttgtg gacgacccga gcgcgactca atactaatcc gacaatcgta
     2581 tgaccagctt cgtgataagc aaccatttcg cgttcttttt tgctgattac gcgatctttt
     2641 ttagctggac ctgcaatcac gcgatcttcc gcttcatcga tatcagaagc atcgattttc
     2701 tttttatttc ttctagcagc tactaacgct gcttcattca agacattttc taaatctgct
     2761 ccggcaaaac ctggtgtctg ttgagccaca actttcaagt caacgtcatc tgccatcggt
     2821 ttattccgtg catggactcg caagattgct tcacgacctt ttacatctgg acgtccaact
     2881 aaaatctggc ggtcaaaacg gcctggacgt agtaacgcag ggtctagtac atcagaacgg
     2941 ttggttgcag caatgacgat tactccttca tttccgtcga agccatccat ttctacaagc
     3001 aattggttca atgtttgttc gcgttcatca tgtccgccgc ccataccggc accacgttga
     3061 cgacctaccg catcaatttc atcaatgaaa atgatagctg gtgcgttttt cttcgctgtt
     3121 tcaaacaaat cacgaacacg acttgctcca acaccgacaa acatttctac gaaatcagaa
     3181 cccgagatcg agtaaaacgg tactccagct tctccggcaa cagcttttgc taataaggtt
     3241 ttacctgttc ctggaggtcc ttctaaaagt acacctgccg ggatacgggc acctaattca
     3301 acaaatcggc gaggatcttt cagaaattca acaacttcaa ctagttcttg tttttcttct
     3361 tccgcacccg ctacatcaga aaaacgtacg cgattagctt ttttgtctgc ttctttggct
     3421 tttgatttac caaagttcat gacacggccg ccaccgccac cgccgccgcc ttgttgtccc
     3481 atcatcatgt aaaagaaaaa gatgatgatt acgattggca agaagctaag caatagggaa
     3541 acccatatac cgctgcttga ttcttctttt actgttgtag caactttgtt ttcttttgcc
     3601 agttctgtta cttggctcaa tgttgtatca tttggcaaaa tgattgttgt gaaatgggta
     3661 gtagtcgtat ctgttgaacc taagatagct aacccaccgc tgttagcgac ggtctgctgt
     3721 tctttgtatt caccagtgat cttatataca ccatttgcag gctgcacttc aaattctttg
     3781 atcttgcctg cttgtagttg ttctgtgaat tttgagtact cgatatctga cgatcgctgg
     3841 ccgttattac caaaaatgaa ataaacgacc attaccatcg ctaagattac gaggacataa
     3901 tacaaggcat ttttcatcat gccgttgttc tttttgttca tagttgccct ccctaagcgt
     3961 caatttaatt taacagtttt ttatcaatcc tgaccattct attgtaccat aatttaattg
     4021 agatacagca ttaatttgat tgataaacac taggtttcaa tacaccgatg tacggaaggt
     4081 tacgatatgc ttctgcataa tccaagccat aaccgaccac aaattcattt ggtacgttga
     4141 agccaacata atctgcttcg atatttacta ctcttccttc aggtttgtca agtagtgtaa
     4201 caattttgac tgattttgct ttgcggtatt tgaaaagatc cactaaataa gcaagtgttc
     4261 ttccgctgtc aatgatatct tcaacgatca acagatcgcg accttctaca ttcgtatcta
     4321 agtctttgac gatttttact tcccctgacg agactgttgc attcccgtag cttgaaacat
     4381 ccatgaaatc caattccaaa tacgtatcga tagaacgaac gatatctgcc atgaatggta
     4441 ccgctccttt taagacacct actactaggg gattggtgtt ttggtaaatt tctgttaact
     4501 ctttgcctag ttctttgcaa cgaacttgta tctcttcttg tgagatcaaa attctctcaa
     4561 tatccttttc caacatcagg ttctccttcc aattgcttct ttttgatatt tgtaaagaag
     4621 tatgtagtgt attttatcag tttcttcacg aatactcaaa tatgaaaagg caaaaggtat
     4681 caagctatat atatttcttt tggcatctgt aatgatccaa gcatgctgcc tttgtgaatt
     4741 aggtactttc tgatcgataa aatatctaga tacttttttt gtgagattct ctgttaattt
     4801 aatgcggtct cctgcttttc ttttttcgac atacagttgc acatcagcag gaatccaaat
     4861 gtcatgagaa aatagagacc atgaatctgt tgctttgatg tcccctgcat tttcaggttt
     4921 gaccagacca atccattctg tttcattcaa atatattcct tgttctggaa tcagcaacaa
     4981 gcgctggtct tctgtctggc tagttgcttg tttttctttt ttagagagta caacaaaatg
     5041 atactctctt ttcagtatcc aattattgcc aatactgagt tgccattggc tttttttctg
     5101 ttgccactgg tctaaaatcg actccatttg ttgttctttc aaaataattc ctgtttttga
     5161 gaatgctttg tcgaaaaagg agtaaaaaaa atagtatcgt tcagcatctg tcatttcttc
     5221 aatcatttct gcagaaaatc gaaattgttc ctcccactgt tcgacttcct tttcgatcaa
     5281 ctgtcccata tatttttgaa tgacttggtc tgcccattgg atctgttgcg aaaattgctg
     5341 gaaatgtctc aacgcctgtg tattttcttg tttcaattga ggaactacca aatgacgaag
     5401 ccgatttctc tggatatcta acaattggtt tgtctgatcc tcaaagtaaa caagctgcga
     5461 ttccgctgca taagtatata ggtcttcttt gctaaaactc agcaacggac gaatcagtct
     5521 gcccgtagca aatggttgta cttctttgat tccagaaaaa gtatttaact gtcccccacg
     5581 aacgattttc attaacaccg tctcgatttg atcatctccg tgatgagcag tcatcaacgt
     5641 gtcatattgt ccttcttcca tcacgcgagt gaattcttga taacgaaagt tacgagcttc
     5701 tgtttctatg gcggatttgg ctggatcttc ccaaatactt acatataaag ggagattctt
     5761 ctcttcacaa tatgagcgaa gataggcagc ttcttctgct gattcttctc ttaattggtg
     5821 attgatgtgg acgataccta aagaaaaacc gatttggcgt tgcacttgta aaagtaaatg
     5881 gagcaaaacc atcgaatcaa caccacctga taccgctgct aaaattctcg atccttgttg
     5941 ccagaatcct tgttcattcc cgtgccgaat aaatgactgc tgtaatagcg gtgctttttt
     6001 cccgcctttt tcaacgttca tcctttccac accctttctt cctaaaaaaa agagagcggg
     6061 aaaacgtcta aaacgacaat taacctgccc tctgttgata ctatgaccca aatagtcgca
     6121 ctatcattat ttgcggcttt ttagctgcga cgtccgccac ggccaccgcg ctttccttct
     6181 gtgtttcgct tcaatgaaga taagcggtca tcgctatctt tcaaaaatga actcatcaat
     6241 gaatcgaagt cttctttttg agctgaaggt tgactgcgag aaaaagattt tttctgtgct
     6301 ggacgatttc ggttgtcacg gggatctttt tgttgatatt ttttaggacg gctttcttct
     6361 ccagattcag caggttgttc ctgcgctttc cggatagata gtccgatttt tccatcatca
     6421 tttactgaaa taactttaac tgttacttca tctcctacac ttaaaacatc atggatgtct
     6481 tttacatagc catttgacac ttcgctaata tgaactagac ccgtttttcc agcacctaga
     6541 tcaataaacg caccaaaatt tgtgattcct gatacttttc cttgcagttt tgctcctacc
     6601 tcgattgaca taaaaaaaat gttcctccta attatttccc ttttttatat tcctgcttat
     6661 tttgcagttg tagaactagt tgattgatct gtttcatttg tcgagctgtc tgtatttgat
     6721 tctaatatag gataaacttt ttcaccgtct tttgagtaaa agaatttgct tctggcaagt
     6781 ttagctacat actcatcatc tttcagcaat gaaacatctt ggtgcagctg atcaacgtct
     6841 gcgttgactt ctttcatttc agtatttgct tcgactctca attcgttcaa ttgatgcatc
     6901 ctttgcatgt catggaaaat ctgcacacct attatcgcga aaatgaccaa agcaccaaca
     6961 aaaattgcag ccagtctgcg tcttcgaaaa atcagttgac gttgttgttg ctgaaattca
     7021 gcatactttt tctttgcata ttccgtatct agcgcagcga ttttattgct ttttttcgtc
     7081 acttggattc gctccaatct ttttgattgt ctttttcatt ataccaatat cgtgaaagct
     7141 tgtctaatct atttctctag tgtttctgtg cgattttcag aaataatttc gtacattttt
     7201 tgtgcatctt cttttttggt tgattcatgt aattccgtaa ttttcacttc caacgttttg
     7261 ttgccaaact ggactttcaa aatatcgcct actttgacat cactggaaga tttcgccaat
     7321 ttaccgttga tttgaattct tcctttatca gctacttctt ttgctactga tcgtcttttg
     7381 atgattcggg aaatttttaa aaatttatct aatcgcatat cttattcctc actttttaat
     7441 ttttttattt aatcgcagga ttttcttacc aaaaggcaac attagccatt ctcgtactgt
     7501 aaacagacga agacgaatca ctgcataaat gaacacagaa gccccgatcc caactcctat
     7561 cacgctgaca aaaaaagcag tggaacgatg agttacagga ccaaatacta tattcaatac
     7621 accataatac agcagcagta ctactgtcat acaaagaagg ctgaacatca acttccgtcc
     7681 aaaatgccgt tctttccaaa aagcatttag ctcttttgtt tcaattcgaa caaaatacca
     7741 aagcgtcaca cctaatccta aaagggtaga caggcttgca cctgctgttc ctaataaacc
     7801 ggtcagaaaa gaagtagtca atccttttac caatagtccc catcctgcgc cttttaaaga
     7861 agggcgaaac gagttcttgc tttgagcaat actttgataa gcttggatga ctgcagtgaa
     7921 agcaatggaa aaaacaaata atacaagcgc tgcatttccc gcataatctt taaacaacgc
     7981 ataattgata taagggagca gcaaggctaa tcccaaagac gcagctaatg ctaaagcggt
     8041 cgtcaatcta agatagattt tggctgtctg taaaaactga cgatagatcc ggttggtcaa
     8101 atgacgagtc aacgcaggca ggaatgtcgc acttaatgca gtggcaatca ctaaacctaa
     8161 ttgtactaaa ggctgacctc gatcatagac cccttttgct atcttcgcac cgtattctga
     8221 cagtccgtaa acttccaaag cattttttac aaagaaagag tcgatcagct ggaaaaagat
     8281 aagcaatcca ctataaatcg aaaccagtcc gccttctacc agaaaacgcc ggatcaaggg
     8341 ctttgacggc tgtttgaaca atggaaaccg tcggaagctt aacgctcctc catgaatctt
     8401 ttgttcatag tagtgaagga tcccatatgc gcataaaccg ccaaaaacag ctccactcat
     8461 tgctgccgtc cctgtttggt acaccgtcca tccaagcttt ctaaaagcaa acgcactaca
     8521 gacaatcaca aaaacacgaa cgaactgctc aactacttga gaaacagcag ttggttccat
     8581 caagaatctt ccttggaaat tccctcgata aaaagataga ccaggcatca gcaaaaaggt
     8641 aaaggaaact acttggatca gcggtttcaa ttgtatgtct cccatcatcc aagcaatcca
     8701 gctgctaaat gcaaagacaa acaaccacaa aaagacacct gtccaaaata ctaagggata
     8761 aagattttgc aaagcttgct ttcgctcgaa aggattcttt ctttctgcaa cgtactttga
     8821 tatgaactgc ggaagaccgg ataaagctaa agtcattgct atcccataaa ttggataaac
     8881 ttgctgataa acataaaaac cttcgtcgcc gactaagttt tggaggggaa ttcggtaaag
     8941 ggcacttaat acttttgcta tgaaagaagc aatcgtcaat acaaatgctc cttgcatgac
     9001 gcgccgcatt tctttatgat ccaaatcatt gctccttcct acgctgacag atatttttgc
     9061 tcacgcaacg ctttgacaaa cttttggatt tcctgaagcc atactgcttc ttccatttgg
     9121 ttggggatcg tcaagcgtat caccatctgt tctttttcaa cgcctagact ggcttttagt
     9181 tttgtcgctg tcagtgcttc gaataactgt tccacactat agcgttttgt tccaacttta
     9241 cttagagtaa agactatatg cgcctcctgt ttccttatcg tttctaggag cgctcgatcg
     9301 ccatccattt tgatctgccc aattgtcaag agatgtgcta cttcttctgg atattctcca
     9361 aaacggtcga gcagatcatc ttctagttca tctagcattt cttgcgaatc aagttcacga
     9421 attcgtttat agatctcgat tttctgccgt tgatctgcca cgtatgtttc aggtagatac
     9481 gcatcgacac cgaggtcgat ctcaacagtg gtcttctcca cttgactatt ctttccttgt
     9541 ttacgagaaa cagcttcgct tagcatttga gaatacatat cgaaaccgac tgcatcaata
     9601 aatccatgct gctgagcacc taagagatta cctgcaccgc gaatggaaag atcccgcatc
     9661 gcaatcttga atccagatcc cagttctgta aaatctttga ttgcctgaag acgtttttca
     9721 ctgacttcgt taaggatctt ctgctgctca tacataaagt aagcatatgc gacacggctg
     9781 cttcttccta cccgccctcg caattggtat aacgtggata atcccatata atcagcgttc
     9841 tcaacgaaca acgtatttgc gttcgggata tctactcctg tttcaatgat ggttgtagtg
     9901 acaagaacat catattgacc ttcaataaat tcaaaaagcg tattttctaa ttgcgcctct
     9961 gtcatttgcc catgagcata accgatccgt gcttccggaa cgagttcttg gatttcttct
    10021 actttttgtt cgatcgtttc tacccgatta tataaataga atacttgtcc acctcgtccc
    10081 atttctcgat ggattgcttc gcgaatagcc ccaggatttt tttccataac atacgtttgg
    10141 atcggataac gattcgcagg cggtgtttcg attactgaaa gatcccgcac gcctaacatc
    10201 gacatatgca aagttctcgg aatcggtgtt gcagtcagag tcaaaacatc cacttgtgca
    10261 cgtaactgtt tcaatcgttc cttatgtttt acaccaaaac gttgttcttc gtcaatgatc
    10321 agtagaccca aatcgctaaa ttcaatatct tttgacaaaa ttcggtgggt tcctacaaca
    10381 atatcaactt gacctgtacg cagttgttcg atggtttctt tttgttgctt tcttgttctg
    10441 aaacgactta atagcccgat atttacgggg aaaccttcaa atctttccag catcgtttca
    10501 tagtgctgct gtgccaagat cgttgttgga actaaaaagg ctacttgttt gctttccttt
    10561 atcgctttaa aggccgctcg aagcgctact tctgttttcc cataaccaac atcacctacg
    10621 agtagtcgat ccattggttt ttctttttcc atatcccgtt tgatctccgc tgcacttctc
    10681 aactggtcat cagtttcaga gtaggggaag gcattttcga attctttttg gtaggcgtca
    10741 tcaggtccaa aagcatatcc tttttctgac tctcttgcgg catataattt gattagatcg
    10801 tcagcgatat cctcgatttt tgaagagact tttcgcttgg ttttcgtcca ttcacttccg
    10861 cctagcttat tgatgcgagg cgctttggct tcagaggcga catatttttg gatcagattc
    10921 agttgagtaa caggaataaa cagtttgtcg tcgttttgat acaaaatagt catatagtct
    10981 tggtgaacgc catctacttc tagcgtttcc atcccaatgt atttcccgat accatgattg
    11041 gcatggacaa cgtaatctcc tgcttttaac tcattatagc ttttcaatcg ctctgcgttt
    11101 gttactgttt gacgtctcgt ttgttttttc gtcgtttttt ggaaaatttc tttttctgtg
    11161 atagctacta ttttttcttg aggaagttca aaaccagcct gcaaagctcc ttcaactaat
    11221 tgtacctgtc ctctgatcaa tgcatttttt tctgtttcta ctactaatat gtcttcatct
    11281 cgaagcattt gttctgcttt gcgtattctg tcctttgtcg gaataaagat cacaacagtc
    11341 tgtttttgct tgcgccaacg gtccatttct gttttcagca atggcatttg tccgaagaac
    11401 tgttgcatcg tccgatattg aaaattatgg attgcttgga aacgaagatt tcccatccct
    11461 ttttggaaga gtgagaaaaa ggttgtggtg aacttttcct tctgcacgat tttttgtata
    11521 tctgctgaaa atgtctgctc agaaaacacc cgcaattcgc tgattttttg agtctgccat
    11581 tctgccgctt ccctttcgat ttcccgattt gtttccatga ttcgctgata atcatcaacg
    11641 actagtaaac tatttgaagg gaaataatcc aagatcgtca cagattcttg gtaaagaaga
    11701 tctgagtaga attttgcttc ttctgtcggg acacctgctt gccagcttga agtcagctga
    11761 ccgaagtaat cttgcaaaaa ctcgcggtct gcttcttctg tggtcgcagc aagccgtttt
    11821 tctagcattt tttgcatttg ctctattcca tgttctagat cctctggtcc gtagacttgt
    11881 tcactcgttg gggaaaccca gatttcttcc gttttttcga ttgagcgttg tgtttctgct
    11941 tcaaaatacc gcatagaatc tatctctaca tcaaatagtt ctacacgaac aggataggtt
    12001 gtcgttaacg ggtaaatatc gataatgctt cctcgaatac tgaattctcc cggttttgcg
    12061 accatctctt ctcgtacata gcccatcaaa gtcaattggc gaggaagatt ttccacgtct
    12121 atctcatcac caatatgcca attcaattgc gcctgtagcc aagttttctt attcggcagt
    12181 cgtttgtgca aagatgcgac tggcaacaaa tagatacctg cttttttttc ttgaagtgcg
    12241 ttaagcgttg caactctctc gcctcgcgct tctggagaag aaaaggccat ttcagcagaa
    12301 agcacttcat ccactggaaa taaatggatc tgatcttgcg gaatcacatg ttgcagatct
    12361 tctgctaact gattcgcata atacaaattt ggcatcagaa taatcatgtt tttatttgtt
    12421 ttcttaaacg ctcgagcaaa tagaagtgtt ttcgctgagc ccgctaatcc tgtgattagt
    12481 tgtctaggca ttttttcttc taaattgctg acccattctt ttacttgcgg atcttttccg
    12541 actaattgaa taatatccat tccttttcct ccttcctcaa ttgaatcgat tcatcgcatc
    12601 cacgaacgaa tgcccttcta tgagatattc gactgcttta acagattgac acatgctttc
    12661 ttctatcaaa ggctgatttt ctttgctgaa aggactaagc acatgctgga ctactgtttt
    12721 tttcccttct ggcctgccga ttcccacctt gattcgatca aagacattcg tattcaaatg
    12781 ggaaataata cttttgatac cgttatgccc gccggcactt cccttttgac ggagccggat
    12841 ttttcccact gccagatcca aatcatcgta gattacgact aattcctctg gataaatccc
    12901 aaaataagtc attagtgggc cgactgcacg accagattca ttcatgaatg tttgtggttt
    12961 gactaaaagg atcttttctc cattatgaaa aaattcggca acttctgctt cgaacggatt
    13021 ttttttgaaa gtagcttgat gctcgcgtgc aattttatcc acgaccataa agccgatatt
    13081 gtgttttgta tattggtatt tggtacctgg gtttcctaac ccgacgatca ttttcatttt
    13141 tgttttccac ctaattctaa aaagcagcac aaaaggctgg actgctaatt agcggtccag
    13201 tccatcgagc ttttttcttt tttacacttg tcaatgcata gtataacaca aaacaaggcg
    13261 tttcggctaa aagagaatcg ttaataaatg ataaagaaat taaaaatttc ttcaaagttt
    13321 aatgaaataa ttgatatata aaaatagtga aataacgcac atgtaagctt ttacatataa
    13381 tacaaccttg ttatctgtta tattttatag ccaaatagcg tgacatgggg cttgagaaat
    13441 gttagcataa gcatgggaaa aaacaaatca atggaaggat tgatactaaa tgactgcagc
    13501 tgcagaaaaa aaagatcatc aaaaagttat tcttgttgga gacggcgccg taggttctag
    13561 ctatgccttt gctctagtta ctcaaaatat cgctcaagaa gtaggtattg tggatatcaa
    13621 tactgctaaa actgaaggag acgcaattga cttgtctcac gctttggcat ttacttcacc
    13681 taaaaaaatc tattctgcta cttacgcaga cgcacatgat gctgacttag ttgttattac
    13741 agctggcgca ccacaaaaac caggtgaaac tcgtttagac ttagtaaaca aaaacctaaa
    13801 aatcaaccgc gatgttgtca cacaaatcgt tgaatcagga tttaacggta tctttttagt
    13861 tgccgctaac ccagtagata ttttgactta ctcaacttgg aaattctcag gtttccctaa
    13921 agaacgcgta atcggttcag gtacctctct tgactcagct cgtttccgtc aagcacttgc
    13981 ggaattagtt gacgttgatg cacgtaacgt ccatgcttac atcttaggtg aacacggaga
    14041 ttcagaattc ccagtttggt cacatgcaaa tgttgccggc ttgcaaatct atgaatgggt
    14101 aaaaaacaac ccagaaatcg atgaagaagc tatggttaac ctattctttg gcgtacgtga
    14161 cgctgcttac acaattatcg agaaaaaagg cgctactttc tatggtatcg cagtagcatt
    14221 agcacgtatc actcgtgcca tcttagatga tgaaaatgct gttcttcctt tatcagtatt
    14281 catgaatggc gaatatggat tgaacgacat ctacattggt gcacctgctg ttatcaaccg
    14341 tcaaggtatc caaaaagtaa tcgaaattcc tttaagtgac agcgagcaag accgtatggc
    14401 tgcttctgct aagcaattaa aagaaattct agatgaagct tttgctaaac tagacgctga
    14461 ataatctttg aacatgacgg aaaacatcta ttcggtgttt tccgtttttt tattttatca
    14521 cccttatggt ttctaaattt tttcataaag tagagataac cttcgtcttt tcctctaaat
    14581 tatgctatca tgagaccgtt ccaccctaaa cttttactta aggagttgtc gatatgacaa
    14641 gatcttcgca tcgaaaaaaa tcgaaacatg ttgctttgct ttcagttctt ggtgttctct
    14701 tagtgctgat cagcttttat acctatcgca gtacatacta cacaaagcat tttttgccga
    14761 atacagaaat aaatggaata aatgtcagca atctaacagt cgaaaaagca aatgaaaaat
    14821 taaaagaagc ttattctgat aaaaaactgg ctattaaaga agatggaaaa gtctggcagg
    14881 aagtaccaaa atccgagctc ggctacaaag atgatttcac ttcggagctt tctccgatcc
    14941 taaatgaaca aaacggatgg acttggggaa tgacttatgt ctcagcggca gaaaaacaag
    15001 aaatagatcc tgtcagtcaa aaccaccaaa aactagacac taccgttcag acattgacaa
    15061 ctaagctgac tgatttgaac aaagaccgga cacctactac tgatgcaacg atcgaaaaag
    15121 caggtgactc tttcgttatc aagcctgaag tcaatggaaa tacgatcgac gtcgatgctg
    15181 cggtcaaaca attaaaaagt gcggtaaact ctggtaaaga tacaattgaa ttgactgaat
    15241 tcaaagaaaa accaaaagta acatctgaag acagctcact gaaagaacaa ctcgcatcca
    15301 tgaatgctat cgcaaatgta aaagctactt acagtataaa cggtgagact ttccagatcc
    15361 catcaagtga tattatgagt tggctgacct acaatgatgg aaaagtcgat ttagatacag
    15421 aacaagtacg tcaatatgta acagatttag gcacaaagta taacacaagt acgaatgaca
    15481 caaaatttaa aagtacaaaa agaggagaag ttacggtccc agttggcact tacagttgga
    15541 cgatccaaac agacagtgag acagaagcat tgaaaaaagc gattctggca ggccaagatt
    15601 ttactcgttc acccattgtg caaggtggca caaccgctga tcatccattg atcgaagata
    15661 cgtatatcga agttgatctg gagaaccagc atatgtggta ttacaaagac ggaaaagtgg
    15721 cccttgaaac agatatcgtt tccggaaagc caactacacc tacacctgct ggagtcttct
    15781 atgtttggaa taaagaagaa gatgcaactt taaaaggaac aaatgacgat ggaacacctt
    15841 acgagagtcc ggtaaactac tggatgccga ttgactggac aggtgttggt atccacgatt
    15901 cagactggca acctgaatac ggtggagact tatggaaaac ccgcggttca cacggctgca
    15961 tcaacacccc accaagtgta atgaaggaat tattcgggat ggtcgaaaaa ggaacgcctg
    16021 tattggtctt ctaacaaaaa taatatataa aaagaaagca cccgtacgat tggtacaggt
    16081 gctttctttt tattatctaa caatgtgcta cttacatttc agcagcagta aagacttctg
    16141 aaacatcatc atcgtcttct agcttatcta ctagacgttc caattgctct ttttgttcct
    16201 catttaaatc aatcgtcgtt tgtggaatca tcgtcaattc tgcttgcgcc aaagtgaact
    16261 ctttttccaa ctcatctcgt acagcagcaa aatcttctgg tgcagtataa atctcgaata
    16321 cttcaggaga agtttgcaga tcttctgccc ccgcctctaa tacatcctca aacatcgtct
    16381 cttcatcaac agtcaaccca tcgcgttcaa tagcgatata tcctttgcgg tcaaacaagt
    16441 aactgactga acccgtttct cccagtgatc caccgtttcg agtaaaagct actcggacat
    16501 ttgtcgctgt ccgattacgg ttatctgtca atgcatggac taagattcct actccacccg
    16561 gaccatatcc ttcatacgtg atttcatcgt aattcgtttc atctacggca ctcgtagcct
    16621 ttttaatcgc ccgttccaca ttgtcgttag gcatatttgc agcttttgct ttgtccacaa
    16681 caagccgcaa agctggattc atagatggat caggaccgcc tgctttagct gccatataaa
    16741 tttcacgaga aagtttctga aagactttcc ctcgttttgc atcttgggca ttttttcttc
    16801 cttggatatt atgccattta ctatgtcctg acatgatttt tcctcccttg ttcttttgag
    16861 aatacacttc tattctagta ctatttcaca aaaaaacaaa gccacagcca atctataaac
    16921 gcttcttgaa tgccgcaaaa agcacacata atatcacagc agtcagagca ccgatcgaat
    16981 ccaacatgac atcttggaac ataggtgttc gtccaccggt catcatctga tggaattcgt
    17041 ccaaacctgc gtaccctgtt gctgaaagcc aagcaaggat cgctgtcagc caccaaattt
    17101 ttagcttggg gaaaacacct aaaaacaagc tgcctcccaa aataaaatag gtaccgaaat
    17161 gtgcgccctt ccttaaaaag aactctaaaa atttggcgta gcccagatgg ttgatactca
    17221 ccatacttcc accataatta aaacgtatac ccgctaatgt gtctttaaat ggctgattag
    17281 gcagccagct ttctaatcgc gaaacttgtg attgctgtcc gtaagtttga gaagaactgt
    17341 aaaataagat tataacgact atcagtgcga ttactaaata aatatttccg tttttcattt
    17401 gtttttttga agaattcata agtcacctct gtattttatc ttagactttt tttgaagaaa
    17461 caacaatcat tttcatagag acttcttcca taaaaaagtg ctatacttaa ttttgtatta
    17521 caatatgaag ggagtttttt catgagttat tcattaaatt ggaatctaga ttctattttt
    17581 tccggaggca gtcattctga tgctttgaac caacggatga agcaattgga agaccaaatg
    17641 aacgagtatt atcaccgcgt aacaaaatgg tcacctagtt ctgacaatgc agagcagtta
    17701 aacgctattc ttcaattgca agaaacgatt acgaatggtt ttacccaatg cagcagctat
    17761 attactgcat tgctttctgc caatgtcaat gattcagatg caaaggtctt atcaggcaag
    17821 ctttacgcaa tgctgcctcg tctgcaatca gccgaaactg tattgtcaaa gaaatttgca
    17881 gaaatatctg acaatgattg gaatcagatg ctttctcaat cttcgtttga aacaattgct
    17941 tttcgtttaa acgagattcg tcgtgatggc tcacaattat tatcagaagc tgaagaaaac
    18001 attataaata cgctttcttt agatggtcta aatgcttgga gcagtcatta tgatacgatt
    18061 gtagccagca tttcgattcc cttcgaacaa gatggacaag tagtcgaatt atctgctgga
    18121 caagccttca ataagatgat gggcgatcca gatccaaaag tcagagaaac acttttcgca
    18181 gcatgggaag aagcctggaa agaaaaggca cccctcttca cggatacttt gaatcattta
    18241 gatggtttta gactttctga ctatgaactt catggtgtca cagattttct acaaaaacca
    18301 ttagagtaca atcggttgaa gaaagaaaca ttgtctgtca tgtgggatac aatccagaaa
    18361 aacaaacaac cccttgttaa ttacttgact agaaaagcta atcttttcgg taaagaaaaa
    18421 atggaatggc aagatcaaga tgcacctatt attttaggcg atttgaaaga aaaaaccttt
    18481 agttttgacg aagcagcagc atttattatc gaaaacttta acaaattcag tccaaaaatg
    18541 gctaaatttg cccaatctgc ttttgaaaag agctggatcg aagcagaaga tcgtcctggg
    18601 aaacgtcctg gtggttattg tactgagtta ccagaaacaa aagaatcaag gatctttatg
    18661 acctacagta attctgttaa tgaggttgcg acacttgctc atgaactagg acatgctttc
    18721 catagcagca cgatgtggga tttaccttct ttaaatcgcg aatatgccat gaatgtcgca
    18781 gaaacagcaa gtacattcgc agaactgatc gtagccgatg ctacattaaa agtcgcaaaa
    18841 acaaaagaag aaaaaatcaa tttgctagat acaaaactgc aaaatgcttt agctatgttc
    18901 atgaatatcc attcccgatt catttttgaa aatcgattct atgaagcacg gcaagaagga
    18961 ttagtctcag aagataagat cacagagatg atggttgagg cgcaaaaaga aggctatcaa
    19021 ggagctttag ctacttacca tccatatttc tgggctgcaa aattgcattt ctttattgat
    19081 gacgtaccgt tttataactt cccatacaca tttggctacc tctttagttt gggcatctat
    19141 gcttacgcca accaaaaggg agcaaatttt gaagaagaat atatccattt actacgcgat
    19201 actgcctcaa tgacgacgga agatcttgcg aagaaacatt tgaatgtcga tcttacccaa
    19261 ccagattttt ggcaagcagg tatcgacatg gtgactgaag atatccaaac gttcatggaa
    19321 ttgacagaag aatttatctg atttctattt tatcacaata aaaaaagagt tagtccagaa
    19381 tgggctaact ctttttgatt aatcattctt acatgctgac agcaaagtca ggggcaaacc
    19441 attgaactga accaactttt acgctttcgc ctggttgagg agcatggata tattgtcctc
    19501 cgcctaaagc aatcgctacg tggtaagttc cgccttgtga cccccagaat aacaagtctc
    19561 cagcttttgc ttgagaaact gaaatttttg ttccagctga ttcttgagga actgtccaac
    19621 caccgatgtc acgaccagtt acttgcatgt aaacatagcg tgtgaaccct gagcagtcaa
    19681 agccacttgg atctttaccg ccccaaacat atggagtacc aatatatttg taagcttctg
    19741 ctacgattgc tgcaccattc acacttccag aaggagctgg agcaggtgtt acaggttttg
    19801 gttgttcagg tgttggtgtt ggcgttggag ccggagtact acttcctgtg ccgtttcctg
    19861 tatcaacact ttgatctgtt gatggtgttg taggcgcagg tgttgttgat tcttctgttg
    19921 ttgaagattc aggcactgtt gttgattctt ctgttgctga agattcaggc actgttgttg
    19981 attcttctgt tgctgaagat tcaggcgctg ttgttgattc ttctgttgct gaagattcag
    20041 gcgctgttga ttcttctgtt gaagattcag gtgttgttga ttcttctgtt gctgaagatt
    20101 gtgtagctga tgatgattct tcagtcattg aagattgtgt agctgatgat tcttctgttg
    20161 ctgagctttg tgcagtagat gaagcttgag tagcttgtgc ttcagcctct gcttgttcgc
    20221 gtgcttcttt ttcagctttt tcttgtgcag cttgttgacg agcttgttct gctagacgtt
    20281 gttgttcacg aatacgagct tgctctgctt cagcttctgc tttttgacgg ttcaagtcag
    20341 ctttcttgtc ttcagcagtt gcttgttcag cagctaatga agttttcagt acgttcaaat
    20401 cagcttgttt agataaaaga tcgccttttt gagattccaa agcagcttgg ttttcagcta
    20461 attcttttag tttagcatcg ttttcagctt ttttgtcttc tacagctttt ttatcttgtt
    20521 tttgttgttc catcaagtcg ttgtttgctt ttaccatcgt tgtcattgct tgaacgcgtc
    20581 caatagcatc tgctaatgaa tcagcattca atacagcatc gatgtagttt gagcttgtgt
    20641 tgcttacttg tgcttcgcgt gcttgttttt ggattgtatc ttcacgtttt tcaatacgtt
    20701 cttgtaaatc tgcaatgtct tttactaatt gtgcagattc ttgacgcaat gtatcttgtt
    20761 ttgctaatag gtcttgtgct tgtgtgttga tttcagaaac ttggctttct aaggcatcga
    20821 tttgtgattg tgcatctgct tgttgatttt tcaagtctgc aatcttttga tcttgctgtt
    20881 gtatctgaga atcaaaatcg tccgccgcgg cagcgatcgg tgatgctacg gcagtcaagg
    20941 tcattgaaca taccattact gctgatatta aactcttttt cactcttcat tcctccgact
    21001 ggcttaattt aataaatatc taaatatatt taagtctttc ttaacgatat aaaaattgta
    21061 ccatagcagt gtgtcaacga tataatagtt atgttacaaa aatgtttcaa aacaactttt
    21121 aaaaatgaca aaactgttcg tttcagaaaa tgatcatgaa aaagccgcgt taagtctagc
    21181 atttcttaag ataacaacga ttctttttcg ctttcgacct aaaaataaaa aaagaaatta
    21241 ttcatcactg aataatttct taaagggaaa aatgaataat acaaatataa tcatgttcaa
    21301 cagcagtgta ggtcctaaaa accttgtaat aaaatacgtg ttatttacta ctgctaattt
    21361 aaaaatcaac tggaccacca tcgtaaacaa ttcataaccc gtgacaaaga tgatcatact
    21421 gaaaaattca gtaaagatgt tgacatggat aacgtctttc attacataca tcaaccatac
    21481 aattaaaggg agagctactg catagatacc gataacgcca atatagtaag cgtcatagat
    21541 agccccgaga acaatcgtgg taatcagtaa ataacgtttt tcaaacgcca tactgcaaca
    21601 taataatgcc aatatcagaa agtgggcatt tgacatatag gtgcctttcg accactcccc
    21661 tagcattctt gttaaatgac catctatcaa catcaaaaag aacaaaacga ttggcagata
    21721 gtacttcatt gtttctttct tcaacatgga ctaatcctcc gctagccgtt ggacaatcgt
    21781 cacaactgac acgtcataca tttctgcata aggtttcaca taaacttccc gatccagccc
    21841 ataactatct ggtttcgttt taataaccgt accgatcggc aagttagctg gagaattccc
    21901 tccaagaccg gatgtctgga caacatcccc ttctttgata tccgtatcac cagttaattg
    21961 ggtcaccact aaggcatgga gcttttcatc atagttttta agcaaaccaa acgcttcgcc
    22021 attagccgaa gatacccgta ctggaaaatg attggagctt tcattagagg atgacagtaa
    22081 ttcgatttta gacgaagccg tattgacctc gatcactcgg ccaatcaatc ctttttgcga
    22141 catgactgcc atatttgctt cgataccgtc tttagaaccc ttgtctacga tcagcatgtc
    22201 ctgccaactg tcaggcgaac gagtaatgac atttgccgtt actttttcgt aactagtcag
    22261 cgtctgattc aactcaagtt cttttttcaa cgcttcgatt tctctctcgt aattcttgtt
    22321 ttgctgtgaa acgtcatcat agctatcgat tttttctttt agccgctcat tttcggcata
    22381 agttgaaaaa agattatgga cagaatctac tccatttcgt acccaccgta ctggggcaga
    22441 gattgtttta tcaaccattg caacactgtc attaacgatg gattggaaga aattgggctt
    22501 tccttcattc gctcgctgcg cagcggtcac acttagtatc gtcaccacga tgataacaat
    22561 aaccaaggta ataataatat ttttattagg attgaacttc ttcacaaaga cacctcgaat
    22621 taattagact cttgtgttca attttatcac taaatgccgt gaaacaaaag gtaagttgct
    22681 cctcttaagc ttttttttag caaataatag attctgtcat ttttagcctg aaagacaaat
    22741 ttttttattt gtttatggat caatctttgc tttgaataac tgcaatcata ccatctgaaa
    22801 aagaaaattc tgcggtatct gaaagaaatt cgttacttgc ataagatgtc ggatattcta
    22861 cttctttatt ttccaataca tacttgctgc cttttaatgt aagattcgag acgggagtca
    22921 agcagcaata agctaaatac ttcatatctg gtatctgtct tactatatgc tctccaggca
    22981 aataataaga caaataattt tgacaatctc tgataataag ctgatgggca aagggtttga
    23041 agcgaggttc catacctaac cagagattgg ctaaaagatg atccaatctt ccgcccgtag
    23101 ctccaatcaa agtgacttcc gcttgcgggt aacgttcaaa tgtttttaag atacctaact
    23161 gtgtatctgt atcgtctttt tctgcagggg aacgggtata ctctttggct tttgcaaata
    23221 cttccttctg ctcttcgttc gacaatgaat caaaatcacc cactgccaga tctactgggt
    23281 agcctgcttg aagcaggtac agcgcaccac ggtctatccc gacataggca tcgtatttgg
    23341 acagcagaga ctgcggccag ttttgcggat tacctccagc agctaataaa attttcattt
    23401 agtctaatgc ctcttttaat cgttcgattt gtccgactgg attttcggct ccatagatat
    23461 atgatcctgc aacgaagaca tccgcccctg catctttaca gatttttgct gtttctggaa
    23521 cgattccgcc atctacttcg attgcataag tccaattgtt ctctctacgt agatcagcga
    23581 gttccgtaat tttttctact gtttcttcaa taaagctttg tccgccgaac ccaggattga
    23641 cagtcatgac tagtacttga tcagccaaag gcaaaacatg tttgatcatg gagactggtg
    23701 ttcctggatt gataactact ccagctttaa ctcctaagct ttttgtcatt tgaagtgccc
    23761 gatgaatatg aggagttgct tcgacgtgga cagtaatgat atctgctcca gcttttgcaa
    23821 atgctggaat gtagttttct ggatcaacga tcatcagatg gacgtctaac ggtaatttag
    23881 taacaggacg gattgcctga acaatatttg gccctaatgt gatatttggg acaaatcggc
    23941 catccattac atctacgtgg atgtaatctg cgcctccttt ttccactaac tcaatgtctc
    24001 tttgtaaatt ggcaaaatct gcactcaaaa tggatggtgc tatcttcatt cttgtttcct
    24061 cctattttcg tttaccttct ttttttgcat acattggttt tcttttttcg atttcttgta
    24121 agaattgcaa ataattgtcg taacgtgtct gtgcgatacc acctgtttct actctatgtt
    24181 ttacttcgca gccaggttct ttagcatgca tgcactctct aaatttgcaa tggacagaag
    24241 cttctacaaa ttcaggaaat tgtctcggca gttctcctgc ttcaattgtc aaaaagtcaa
    24301 tcgagctaaa tcctggtgta tctgctacta acccgccata gataggaatc agttccacat
    24361 gacgtgttgt atgccgtcct cttccaagtg catcagaaat ctctcctgtt gctaaattca
    24421 gttccggtga tatttgattc agtagcgtcg attttcctgc tccagactga cccataaaga
    24481 ccgatagccg ttctgggaag taacggatca gttctgtcgt atcacccgta tgattgggta
    24541 caatcaccgg atagcctatt ttagtatagg tttgcttgat ctcttcgatt ttttctgggt
    24601 cgtctgacaa gtcggtcttt gtcaaaaaaa taatcggttc gatcgactcg tattccaagg
    24661 taactagaaa gcggtcaagt agattataag aaaatgcagg ctctacgata cttgtgacaa
    24721 tgattccaag atcaacattt gtgactggtg ggcgaactaa ttgatttttt ctcggcaaga
    24781 tttccagaag atagccgtct gtttgatttt cactctcaaa gatcacctga tctccgacaa
    24841 gcggcgtgat tttccgattc cggaaatttc ctcttcctct tgtttgatag gtctctccgt
    24901 ttgcgtatac atagtaaaaa ccgctgattg cttttctgat ttgtcctttg ataagtgcca
    24961 cctccgtttt tgtttatttt accatacgaa gaggaaagta ggtgttattc catgcaaact
    25021 tctcaaagta tatggcttga aaattgatca aaagagatag aaacacaaaa aagagaatgc
    25081 aacaaatcaa tgtcgcattc ccttctgata tattttattc agcacttatt ttgaactttc
    25141 tgaggtgcta gctgatgaac ttgtggagct ggaagattct tcttttgaag agttgcttgt
    25201 gctactggag ctacttgagc tgctagaact gtctgaagag tctttttcag aactagaaga
    25261 cgctggatct ggtcctttag agtaaattac gttcacaacc attcctttgg taattgtcgt
    25321 tcctgcccct ggtactgtac gaatcacttt atctttatcc accgtgtttg aatattcgga
    25381 agtctcatgc ccgatatatt ctgcaccgac actagctaaa taactttgtg cttcgctttt
    25441 tgtatagccg gagatatcat tcaatttagg ctctgttcct tggcttacga caaaattgat
    25501 gtaagtttct tctgctacaa cttcacccga ggctggatcc tgactgatga tcgaacctga
    25561 tggtacagtg tcgctatagt cataagtagc actaatatta ttgatattga atccttgtgt
    25621 aacaagccaa ttacgaacag tttcgtagtt ttctcctaca tgactactca aagtcactgt
    25681 ttctggtcct ttgctgactg tgaaagtaat cgtgtctgtt ttaggatcga cttctgtatc
    25741 ttctgatgga gattgcgaaa tgatcattcc gctttctacc tctgaactga attcctcttc
    25801 tttttgaatc aagtcagcag aaaaccctaa ttctttcaaa cttgcttcgg catcttcata
    25861 acttttacca ctgtaatcat ctaatttgat ttttttcgtg ccgctactaa tatacagagt
    25921 gacttcgcgt ttttgcttga ctgtcgttcc cgctgccggg tctgtcttca ctacattacc
    25981 ttcttcgatt ttgtcatctg caatttcttc tgtttttgtt gctacttcta atttcgcttt
    26041 tgcgagtgtt tctctagcag aagcttcaga aagatcagaa acatcaggaa caacaacttc
    26101 tcctcgcccg ccagaagcaa agaatacagc tgttccgatt cctaaaacag caagaatagc
    26161 taataggatc agccaccatt ttttctgtcc tttcttttta ggatcaggca gaacttcttc
    26221 ctcctctggt aaggtttcgt tttctggctt gtcttttggc agaggcatag atttgaatga
    26281 atcaggcatt ggtgcatctt ccgtaagggg tgtgataact tttgtttcat tatccatcac
    26341 atgaggctgc catttttctt cgtttgcacg agctggtgat aatactgtag aaagatcatc
    26401 agacatttcc tcggctgact tataacggtc tgcaggttct ttagccgttg cccgtaaaat
    26461 cacgttttcc agtgcttgtg gcacatttgg atcaagtgct tttatagatg gaagatcatc
    26521 ttgaaaatgt tttaaagcaa ttgttacagc agattcacca tcaaacggta cgctacccgt
    26581 caacatctca taaaggatga tccctaaagc ataaatatct gactgcttcg ttgccatact
    26641 tcctctggcc tgctcagggg acagataatg aacagatcct agcatcgtat tcgtctgcgt
    26701 gatcgatgtt tcagataaag cgatagcaat cccgaaatcc gtgattttta caacaccttc
    26761 attatcgatc agtacgtttt ggggtttcaa atctcggtgg atgatttgat ggttatgagc
    26821 taaagaaata gcagaaagga tctgctgcat aatatttact gctgtttcat aaggaacagg
    26881 ataatgtgtt tggatatatc gtttcaagtc cattcctttg acgtattcca tcaccagata
    26941 ctgcatgttg tcttcttcac caacatcgta aacactaaca atgtttggat gaacgagttc
    27001 tgttgctgct aaagcttctc tctgaaatcg tcgaatcgca gtctggtcat tttgaaagtc
    27061 aaatcgtaag actttaactg ctacatcacg atccaagatt aaatcgtgcg ccaaaaatac
    27121 atttgccata ccaccgctac cgatatttcc agtgatttga taacggccat ttaatttttt
    27181 gcccaactcg atcatacact gtcctcctta tattcgataa gaagaacggt gatattatcc
    27241 gctccgcctg cttcattggc acgatcaatc agctcagtta ctttatgaga caatgggcct
    27301 tcttgattga tgatcgttcc tatttcttct tcagacagca tgttcgtcaa gccatctgaa
    27361 cataacagca gtcgatcttt tagttgccaa agatgagtag aaacatctac ttcgactgtt
    27421 ccaggcatgc cgacagaacg tgttaaaata tttttccttg gatggttcac tgccatttct
    27481 tcgctgattt ctcctgattt gactagttca ttgactaaag aatgatcctc cgtcaattgc
    27541 atgattttat cttcacgaat caaataagct ctgctatccc caacattggc aatcgtgaac
    27601 cagtcatctg acagagcggc cgctacgatt gttgttccca taccgttata ttccggctgt
    27661 tcttgtccac gctgataaat cagcatattt tcttcttgaa tcgattgaat gaaccattgg
    27721 gcgatttttt catcgtcaac agcttcttgt ttttcccaag cttcgcctag attcgttacc
    27781 gccatctgac tggcaatgtc tccggcacga tgaccaccca tcccgtctgc taatacagca
    27841 agcttgagac ctgaacggct ggtaaataca ctggcatagt cttgatttgt gttacgtcgt
    27901 cttcctacat ctgattgata ttcaatctgc attttttcac ctcattactt tttgcgcaaa
    27961 caacaaatga aaaatccatc tgtgtaaaac tgatgcggat acaaagtcaa cattttctct
    28021 tcgattgatg attgtagcat aggattggtc aaaacatcaa tcctctcaaa atcagggtgt
    28081 ctttccaaga aagcagaaac aacgtcttgg ttttcttctt ttaagatcgt acaagtgcta
    28141 taagtcagaa taccggaaga ctttaaagtt ggtgcgcagc tttctaaaat ttccaattgg
    28201 atttttggta aattcgctaa ttcatccggc tgttttttat aacgaatatc tggttttcgc
    28261 cgaagcagcc caagacctga acaaggcgcg tctactaaaa tccgatcaaa ctgctctgct
    28321 tcgaactgtt tactgacttc tctagcatcc atcttttgag cgtagacagc agaagagaca
    28381 cctagacgtg ctgcattatc ttcaatgagt ttgactttgt gttcgtgtac atctaaagcg
    28441 accacttctc caccgcatga cggatctaaa aaagaagcaa tatgcgtcgt cttacctccg
    28501 ggtgctgcac aggcatctaa gacatgatgt tcaggctcga tttgcataga cggcgctaca
    28561 agcatcgaac tttcatcttg gatcgtcatg acgccatcgt ggaacaatga gctagaagct
    28621 aaatgccctt ttttcgctac tataccgtat ggactgactt ggcttttttc agctgtgatc
    28681 tcttcttctt gcaaaacagt gatggcctga tctctggaaa gtcggcgtgt atctactcgg
    28741 gcactagcat ggctaggctg atatagcgat aaaccgagct tttcagtttc ttcgtatccg
    28801 atttgatcca ccagtcgttt cgtcatccat agaggcaggc tgattttgac agaaagtctt
    28861 tcgataggat cagcaatgcc ttgcagatct ggtgctcctt gacgctgata attcctcaag
    28921 acaccattta caaatttccc tgtcccagga tttcctttta cttttgcgat ttcgaccgcc
    28981 tcgtttaaaa tagcatgtgc agggacttta tctaaaaatt ctaattggta tacagaaaga
    29041 taaagaagca tcttgaccca gtcatccact ttttttgctt tagcgatcaa tggtgcaacg
    29101 taaaattcca atagtcgctg atggctaatc gtaccataga tcaattctgt aaataaaccc
    29161 acatcttttt gatttagctc gctttgtttg atcatctcat tcaacaaaag attcgagtag
    29221 gcgccaccct tgttcactcg ctctaatgtc agtaatgcca tatatcgtac tgattttttc
    29281 atttttttag ggattttttt cattatttca cctgctgacc aatctgaact tgctgaccgc
    29341 ttccgttaag gaaatcatgt acggcctgtt ttcctttacc agctggctgt aattcattga
    29401 tggctaagac ggtctgttta ccgcaagcaa tccatagatt ttttttgtct ttccggatga
    29461 tcgtaccagg tgctttgtct gtagtttctg ccaatacttc aaccgataat aatttccagc
    29521 gtatgccctc ataggtcgtg aatgcaattg gccaaggacg cattccgcgt acttggttgt
    29581 caatttcttc tgcagtttta tgccaatcga ttgcttcttg ttcccgtgtg atgtttggcg
    29641 aaaaagttgc ttctgactcg tcttgaggta ctggtttttg tccatccaaa atattgggca
    29701 atgtttctag caatagcttt cttcctaaaa ggctaagctt ttcaaacatc gtccctacat
    29761 catcttgttt agtgattggt atgctttctt gcgcatagat tccgcctgca tccatttttt
    29821 tgatcatttc catgatcgtt acaccagttt ctttctcccc attcatgatg gcataatgaa
    29881 caggcgcacc tccccgatat ttaggaagta aagaagcatg gacattgatt gcccctaatt
    29941 ttggtacttg caataacttt tcaggcaaga attggccaaa tgccgcagtg acgatcacat
    30001 caggcaccaa ttcaatgatt tcttccattt ctggcgaacc ggagattttc tctggctgca
    30061 gcacacggat accgtgcttc agagccgctt tttttaccgg tgtcggcgta attactcgtt
    30121 tccgcccaac tggacggtcg ggctgtgtta ccactgcctg gatctcgtaa cctgattcta
    30181 tcagcccttc aagaatcggc acagaaaaag caggtgtgcc cataaatact agcttagtca
    30241 tcaagatgtt cctccatata ttcttcaagt tcttcttccg ggatctgctg tatcattttc
    30301 tcaatgaaca agataccgtc taagtgatcg atttcatgct ggaaggcacg ggctaaatag
    30361 ccaaaagccg tcacttctat ctcttctccg tctcgatcat aataacgcac ggtcacttca
    30421 tctgctcgct tgaccgttcc atagacatgc ggaatactga gacaaccttc tacgtcaagg
    30481 ctttcacctt ttgcttcgat gatttctggg ttaatcagtt caaatttctc gccttcatct
    30541 acttcgatca cagcaattct cttgttttgt cctacttgag gagcagcgat ccctattccg
    30601 tcattcgcaa tcattgtctc atataaattg tctaataaag cgattgtctc gtccgtgatt
    30661 acatcgattg gctgagcagt tctttttaat ttatcattag ggtggatcaa tataggatag
    30721 cgcataattt ggccctttct taaataaagt ttaatggttc gttgtcaatt gccacaaaaa
    30781 gaccttgtcg ctgttctttc tggcttccat tcaaaatttc atccaaaaca tgaggaagat
    30841 tcggttcatg tttatatttg atgattagtt gataataata tcggtttttc actctcaaaa
    30901 tcatgctggg cgttggccct aatagcaagc tttgagggct taacacttct tttagctgat
    30961 tggcaatctg aaagattttc tttgccgcta cttgttcttc aggatggctg gcggttattt
    31021 tcacagtaaa gtaataagga ggatagcctc cacgatgccg tacatgcatt tcctgctgat
    31081 aaaactgctc ataattttgt tttttagcca attctatggc gtaatgttga ggattgaatg
    31141 tttggatcac gacttcgcct gctttttccg ctcgccctgc tcgtcctgaa acttgggtaa
    31201 gcaattggaa tgtatgctca ctagaacgaa aatccggtaa attcagggag gtatcagcat
    31261 tcaacacccc aactaatgtc acttctggaa agtccaaacc tttagcaatc atttgtgtcc
    31321 ctaataatat atctgcttct tttgcaccga atttttgtaa aatctgttcg tgtgcccctt
    31381 ttcttcgagt ggtatctaca tccatacgca aaatcctagc ttgcggataa agttctcgga
    31441 gttcctcttc tactttttga gtacctgttc catagtagcg gattttattg ccgccacaat
    31501 taggacagcg attgggaata ggttcttcat gtccgcaata atggcagcgc attgaacgtg
    31561 tatccatatg cagtgtaaga gagatatcgc aattaggaca tggaagaaca aatccacagt
    31621 ccctacacat cacaaaagac gaatatcctc gacgattgag cagtaggaca gtttgttctt
    31681 ttttgtctaa tcgattttgt attttttcct gcaaattagc agaaaaagta gaggtccgat
    31741 gattctgaaa ctcttcacgc atatctacga tctcaatagc tggaagctgt gcagctgctt
    31801 tggctcggtg gtttaactgc aataactgat aaacgccttt ttgagctcgt gctcttgatt
    31861 ctaaagaggg cgtcgcgctt cctagaacta ttgggcaatg atgatattta ctgcgccaaa
    31921 ttgctagatc tctcgcatga taacgcggtg tctcatcttg tttgtaactt gattcatgtt
    31981 cttcatcgat aatgatcagc ccgatatttt tcagcggggc aaaaatagca gatcgtgcgc
    32041 ctactactac ttgcgcttct tcccgctcaa tcttgcgcca ttcatcatat ttttcacctt
    32101 gggaaagtcc gctatgtaag accgcaactg attctccgaa gcgtcctttg aatcgctcga
    32161 ccatttgcgg agtcaatgcg atttcaggca ctaacataat agccgtttta ttttctgaca
    32221 aaacatcagc gattgcctga agatatactt ctgttttccc gctgccagtg attccctcca
    32281 gcaaaaagac ctgatctttt tgctgctgac ctgctgtgat gatttgttcg acagcatttt
    32341 tttgttctgc attcagttcc aatgccgtcg tttggtcaaa tactcggtct ttataagggt
    32401 cgcgataacg ttctgattct ataaactcca gccagccctt ttcttttcct tgattcaaga
    32461 cagcagtact gattccttta tttttgaaat aagcaattgg ttcttcttgg ctcagttcag
    32521 ataaacacaa aagaagttct ttttgtttct tggcattttg ccgtaatttt gcccattctt
    32581 cttcgatttg cttttcagtt aacaaagaac ggaccaaacg aatcgttttt actttatttc
    32641 gtgtatggac ttcatatttg atatctacta attgctcttg gcgccagcga atcaattgag
    32701 aaaggctgcc ttcttcttct gcttccttcc aggaaatcac ttctcgctga tcaaacaata
    32761 cctgaacttc tggtgtgtct gccaaaggat agatcaattt atcatattct gctttcatca
    32821 cactcggaag catcgtttgt agacaagtga ttttgaatgc gtaagtaatt tttttcatat
    32881 aatcagctaa tgaaagtaat tcttcattga ccaccggagc aaggtctagc aaacgaataa
    32941 ctgccttcaa agatggatta gctgattctt ctgatttttg gatagccata acaaatcctt
    33001 ggacatgacg attgccatta ccaaaaggga cttctactcg catcccgact tgcaaagctg
    33061 tttccatttc agagggaacc aaataagtga atggctgatc ggtctgcata gtaggtacgt
    33121 ccacaatgac ttcagctgtt aatgtcatgg ttcgtttcct cctttttttg tcttttctta
    33181 tcaagcgaca aaaagttcct tgcctatttt actagagatc gatggatttg tcttatcttc
    33241 tgccaaatcc taacaaaaaa ttaacagcag ataagaaaaa aaagaactgt gtcaaaaagt
    33301 tcagacacag ccttttctac cagacaataa gtggcgaatg tttgaaaagg aaacagctgg
    33361 gtttgctttt ctttttagat gttcgctcta tttttcttta ttacattttt tgctcttcac
    33421 ggatacgtga ttccaattcg tgttgttcac gctctttttg tgcttgacgt tcctcttctt
    33481 ccatcttcaa acgttcacgt tttaattcag gatgtggatc attgactaca tttcctgcat
    33541 cgatttcttc taaagcttga ccgacgcttt ttactgaatc aaaagaatct agagtaggct
    33601 gtgcacttgc atccaattca tgcgcgcgtt tacttgctaa aatcactaga gaatattttg
    33661 aatttacacg atctaatagt gagtcaattg aaggttttaa catcataaga ctacatctcc
    33721 tttaacattt taatatattt accgattacg cgatctactc gaaaatgttc actggcaata
    33781 atgtttttga ttcgttcaac tgctagcggc acttcatcat ttacaacagc atagtcgtaa
    33841 agagccatca tttcgatttc ttcttttgca actttcattc gtttttcgat tacttcatct
    33901 gcatctgttc ctcggcctac gatccgtgat tttaattccg ctaaatctgg cggagtcaaa
    33961 aagatgaata caccatcggg cactttttct ttgacttgtt ttgctccttg gacttcaatc
    34021 tccaagaaaa catcttttcc ttcatccaat gtcttgttga catatgacaa aggagttcca
    34081 taataattcc cgacatattc tgcgtattcc agcatctctc cagcttcgat cattgattca
    34141 aactcttctt tgcttcggaa gaaataatca acgccttcga cctccccttc acgcatttta
    34201 cgggtagtca ttgagatcga atactgaaaa tcattatcct cactctcaaa aattgctttt
    34261 cgtactgtgc cttttcctac accggatgga ccagacaaca cgattaataa tccacgctct
    34321 gacatgacga cgtcctttca actagtcttt ttgtacttat cgtttatttt gcactttttt
    34381 tctgcaactt tcaaggttat ctttaattat agctagaaat tggtaaaaaa gctaccctgt
    34441 accaagaggt cgtaaaaaag ctgtcctgca tcaagtaata agaacagcca aacataaaca
    34501 gcttctcatg tttttcgttt ggttggggtt attatcgcag atgcaagctt ttgaacgccg
    34561 tgtataagag gtcgtgaaga agccgattag tttcgagtat gaagaaaaat tttgtgaaaa
    34621 tagtttctca catttttgca taaatttggc ttaataccga gaaaatggtt tttgaacatc
    34681 acttattcaa aataaaaaaa gattgtgaca cgaattttgt cacaaccttt aaatgtattc
    34741 ctagagcaaa gaagctaagc gaagaaatcc aattctgttt atttattgta tttctttgtt
    34801 cagcatcctt gctaaaagcc catattggac aaagtattca ctacacccat gatctcatct
    34861 tttcgagaac gtgctttttc tgtcggatct tctacctctt ctgtgtcaat tcgaacaata
    34921 aatttccgat tcatcgtcac gtaagcaccg tcgttgatca aaataaacag ttcgttctca
    34981 ttatagtctg cacaggcatc tttccatacg tcaaagccgt ttttttcaga agggatatcc
    35041 cgtttaacca cagtttttcc acttaattcg tggaacgtta cgtcataata tttcattatc
    35101 tctacctctt tcgtcattca atattttgta cttgttctct gattttttct aagatcgtct
    35161 tagactggac gacttgattt ttaatttcga ttgcatttga tttggaaccg atcgtgttga
    35221 tttcccgatt catttcttgg atcaggaaat ctaattcccg acctacagga cattgtgctt
    35281 cgataagttc atctagtttt tgtacatgga tcgccagacg atccagttcc tcgtgaatat
    35341 ctccgcgttc caacaagatg gctagctccg tcaacaaccg ttgctcatcg actttcgctc
    35401 caagaaattc ctccagcttt ttttgatacc ttatttgaaa ttccttttca tagatttcta
    35461 caaaactatc gagttttttt agtacttctt tgaattcttc agtattcttt ttcaacacta
    35521 aagctaaagc ttgaccttct ttttctcgac tcgcctcaat agaatcaagt gcgcttttca
    35581 atgtatctag agctaactgt gccaattctt ccatgctctc ttccggtttt tcttctaccg
    35641 ttacgaagtt ttcgtccagc acgaagcgtt ccgccatccg actaattgac acttgatttt
    35701 gactaccgta tcgttgttga attccgtctg tcaattgagt gataagtgaa tcaatcaatt
    35761 cccagttgat gacgatctgt ttttcgtttt tgccaatata agctagattc acaaatactt
    35821 cgattcgacc gcggcttaac tgctgtttaa taagctgacg aaaatcgttt tcaacaaagt
    35881 tcaggatttt aggacttcgt atttgaacat ctagaaaacg ctggttgaca ctttttattt
    35941 caatatctaa ctgatacgct tttgtttcgc ggatggcttt gccaaaacca gtcatacttt
    36001 tcattcgaca gggtccttcc tcattacata ttttctattg ctttttgcag ttgttcaaac
    36061 tcttcgttcg tcaatgtcag gcctttcccc attttttcat ggtctggcgc ccaatctcga
    36121 agatcgaatt ttggcgggcg accattccag ctgactaggt tcagttcttt acgccacccc
    36181 ttgttgtttt ctgacaatat agcgatttct tctacgattt catatgaaaa ttcttgtgcc
    36241 atggttgttc ctccttgaga taatataaat ataggatcag taatgtgtca actggttgct
    36301 taagcaattc atgctgcaaa cgatccttgc gttttccttg tagcagataa tcaaaataaa
    36361 cagttttcgt gcgctcattt tgtatttttt gctggtattt tcgatacctt cgccattctt
    36421 cagtacttaa ttgaccaatt tgtgccaata aatcatccgg catacttttt atatcttgtt
    36481 ggcagatcat ttgaatcatc atgtctactt gatagatttc attgggcagt aaaaaatgtt
    36541 tttttgctaa aagttctagc atcgccaatc cgcatgtctc tggtacgacc ctgttcttat
    36601 ataaattacg cagtttttgc caaaatttta tgcttttgaa aaaagataat tctaactgaa
    36661 tttcttttgt taagtaagac aaaaatcctc gccagtatgt tcttgcaggt ttaacagaat
    36721 gaaatgtata ccagttccca tagtaatagc ctaatcgttt tttcatttcc agatagccta
    36781 attgtaaatt taattggttc ctttggctat caaaaagcag aaagctgcct agtgtccaca
    36841 atgtttgaca tttccaatgg ataaaaatgt ctggcatacg aatttttttc gctgggcctg
    36901 gcccttgcac atctacgaca aatgcctctg ttgctccttt gttaatcgca atatcaatag
    36961 gtattttgtt gcgataccca ccgtctgcat atttattttt tccgatcttt cgataagcca
    37021 tagcgggata aaatgaagca gaggcgagta tccaatctgg tatttcttct gtttttgtct
    37081 gttggatatc agtcactact tccgctagtt ctggcagttt agtagacacc gtatataaac
    37141 gtattttggg attatgtttg agtaaggaca aatccaaatt gtctttgacc atcttttcca
    37201 atggagcaga agaaaccccg ccttcaatta gtgctgtttt cgtcatttgg cgtctttctc
    37261 gatagaagcg ttcacgaagg tcttcaactg cagccatttc tggtaacgca agcacttgtc
    37321 ttgtcgacag ctttttccat aaattcacag cttttttcat atctccttgt aaaatcaatg
    37381 cgccgttcaa cgcacctacc gaggtacctg tgataatctc aaagttgata tcatgttcct
    37441 tcagcgcctg ccacacacca atctgataag caccgtgcgc tcctcctcca cctaaaacaa
    37501 gtgctgtatg atagaccagc tttttattta attgtgtggt tccttgatag ccgtttttat
    37561 gaaaaatacg caaccattct tctgatagag gagattgaaa taaaaaacaa cagttttctt
    37621 taataaaaaa acgagcgcaa gcttccatat accgaaaaaa ttttttccat tcaaaagttt
    37681 caccttcaat caacagatta ttaaaaataa catctcctct acttttagca atagtaaaat
    37741 agacggtata ttttttcgat actgctttaa aagcacattg cttttttgca gaacgccaaa
    37801 aagcgtctct actttcttga tgactttgaa aaaatggaag gggttgtttt gcttgctgga
    37861 taggcgaata cagcgacatt ttctgtgaaa agtaaacttt ttttatctcc atcgcgctct
    37921 cctttttctt caagtatacc agtacgtcat aaaaaggtca tgagtctcgt attcgacttt
    37981 gtagattcca taagaaaaca attcataaaa cctcctttaa ataaaaaaat acaaaaatca
    38041 caaaaattaa ttgaaaatga gagtgttttc aatgaaaacg tgattttgta atggaatttt
    38101 aaaataaaag aaacaagctt ttttaatgca aatgttgtaa aataaaagag attaagagga
    38161 aataagagga ggaacatgat gactgagtca gtttatgtac atattgatac cacaagtaat
    38221 gctgttttaa caaaagggct gactgctact gactttgcaa acagtatcgt gcattttcct
    38281 caaaacttgc tcttgcttga tccatctgct tctgctggag aatatgaatc ccatacaggg
    38341 ctgaaagtaa tccgtggtac agaaaatatt cagcgtttct tttcctcttc tcgtaacagg
    38401 cgtggattcg atgaattgaa gtggatcgac tttactgatt taacgatgct gaaagaattg
    38461 acacctttag aaatttcaga attattatat tttggtcata tgaaaacaca tcttcattcg
    38521 ccgttttttt ataaactaca gaataatttt gtatacttcg atcttaatga tcagctgaat
    38581 cggatttatt atcgctattt agatgaattt tataggattt ttgctagtaa attaagttcg
    38641 atcttgtcag ataagatcaa tgtaaaaaaa tcttttttca aaaaagaaac acctgttgaa
    38701 aaaatcagtg tggaattatt gaaagaaatg cgtaatttga tgcaggaagg gatcattttt
    38761 tcttttgatc aagttggttt gatcgatggt aaatatcgta tcccaatcta tgtgattgaa
    38821 gattatattt ggaaattaaa aggcacccgg tatcgagaag aggaaatcat cgctacttta
    38881 atctactctg tcaccgatcg acactggcaa ttggtagaag atgccgatct ttcttatctg
    38941 ccattgaagt agatctattt cttatcaaaa aataaaaagg aaacaagaaa ctaggacatg
    39001 attctgtctc aagtttcttg cttctttttt atttacagct cctgtcgttt atctggcaca
    39061 aaaatcgaag gagtcgtttg ttggatccct aataaaacga tgatcgttac aatagctgta
    39121 ctcaacccgc tggcaccatt cattacgaaa gaaaagagcc aaggattcat cccccatagt
    39181 gcatacgctc cccagaaaac cactcctgca ataaaatgcc aaaaatatct ggaaagtgcg
    39241 ccaatcaatg caccaaacac gatgcttttt ctaatttgtt tcgtattatc gtctacatag
    39301 cctttttgga catttgaagc catgagccct gctgcgcctg caaaagtata ggcaatcggg
    39361 tactcaatca acacttgaac gacacttaaa taataaactt gtcccaacgg aaaatgtagg
    39421 atgccccaaa tgaaacctgc cagcatccct gcctttgtcc ctcttcttaa cgcaaaaaga
    39481 gtcaatggaa tctgccctaa tgaaatagaa aaactgctgc cgatctggat cggaatcaac
    39541 gacaacacca tcgctaacgc tgcaacaatc gttccttcta cccaaactct attttttccc
    39601 ataaaaaaac ttcctccttt gaataaaaaa gtcacaaagg aggaagcagc tttcgaaaat
    39661 agtcaaacag ctgtttattc acaattccta cgctcgcata accgatacag gtacgaaggg
    39721 tttagaaata gattcaatct cagcccaatt ggactcccct ttgtgagttt atttattatt
    39781 tattttttgt ttcaaacaat ggactaactg gtttgttttc atggatacgt ttgattgcat
    39841 ctcccattag tccgccaaca cttacttcat cgattttgtc gattttacga tcttctggaa
    39901 gatagatcga gtctgtaaca actagacgct cgatagctga atcttctatt ctttgtaaag
    39961 cagggccgga taatactgga tgtgtacatg aagcatacac actagttgca ccagcttctt
    40021 tcaaagcatt ggcagccaat gtgatcgtac ctgctgtatc gatcatatca tcgatcagta
    40081 cacaagtttt gcctttgact tgtccaatga tattcattac ttctgcaaca tttgcttttg
    40141 gacggcgttt gtctatgatc gcaatcggag atttcaaaaa ttccgctaat ttacgtgccc
    40201 gtgtgacacc accatggtct ggtgaaacaa cgacaacatc atcgcctttg attccttttt
    40261 ctaagaaata attggcaata agtggcgcac ccatcaaatg atcgactgga atatcaaaga
    40321 aaccttggat ttggactgca tgaaggtcca aagtcagcat tcttgttgca ccagcttttt
    40381 gaatcatgtt tgctactaat ttcgctgtga ttggttcccg tgcacgtgct ttacggtctt
    40441 gacgcgcata gccataataa ggcatgacta cgttgattgt ttttgcactt gctcgtttca
    40501 gtgcgtcaat catgatcagc aactccatca agttatcatt gactgggctg ctcgttgatt
    40561 gaataacata gacatgcgat ccacgaatac tttcttcaat gtttacttga atttctccat
    40621 cactaaattg agtaacagat gattttccta actcgacgcc gactgctgct gcaattttct
    40681 ctgctaatgg gcgattggaa tttaacgcaa aaatttttaa tcttgggtca aagtaatgtt
    40741 ttgacatggg gacctccact ttctttttca caactctaga attctaccta ataaatagta
    40801 gtcatatttt ttaaataaat caagtgattt tgcaaaagtc ttagtttaga taagggagtt
    40861 tttttgcata tccttcttta ttgacttgtc ttgctctcgc aattgccagc gcatattctg
    40921 gaatattgtc tgtaattgtt gacccggcag caatcgaact gtttttagct acctcgacag
    40981 gtccgatgat attcgtgcta gaaccaataa agctgtggtc gccgacagtc gtatgatgtt
    41041 tgttctttcc atcatagttg acgaagacaa caccacatcc gacattgatt tcttctccca
    41101 gcgtagcatc accaacataa gtcaagtgac cgactttagt tcctttgccg atttgagcat
    41161 ttttcacttc cacaaagttt ccgatatgga cgttttcgcc aatttcagct tttggtcgta
    41221 aatgtgcata aggtcccaca tctgcatgtt tcttgacatg gctgctttca atgactgagt
    41281 tttcaatgac gacgtgatcc tcaatcctac tgtctacgat ttttgaatgt gacccgatga
    41341 cacaatcaga accaatgact gtatttcctt ggatttgtac acctgcttcg atcaatgtgt
    41401 ctggaccgat ttctacgcct gcatcgatgt atgtcgttgc tgaatcgaca aagcttacgc
    41461 cattgaccat atgcatctgg ttaattcgct ttctcatgat ctcatttgct ttagctaacg
    41521 cgatccgatc attgactccc attgattctt caaaatcatc cgtttgataa gcagcaactg
    41581 tatggccttc ttcttttaag atttcgatga tatctgttag atagtattca ccttgagcat
    41641 tgtttgttcc aactttgctt aacgcatcga acaaagcttc gttgtcaaaa cagtaagttc
    41701 ctgtattgat ttcttgtaca agagcttctt ctggtgttgc atctttttgt tccacgattt
    41761 tttctacgat cccaatatgg tcacggatga ttcgtccata tcctgttggg ttttctgctt
    41821 gtgccgtcaa gatcgttgca cttgcatttt ttccttggtg atattcaaaa agattgttca
    41881 gcgtttcagt agtcaataat ggtgtatcgc cactgataac taacgttgtt cctttttttc
    41941 cttccaagaa agaagcagct tgcaaaacag catgtcctgt tcccaattgt tctgcttgta
    42001 acgcatattt actgcgttct cctagttgtg ctttaacttg ttctgctccg tgtccaacga
    42061 tcgtaatcac ttcatctggt ttcgtttcac tgacccgatt aatgatatgt tctaccatag
    42121 gtttaccaga aacgggatgt aaaactttat ataattttga cttcatgcgg gtacctttcc
    42181 ctgccgccaa aataattgca taacgtgcgt ccaactgcgt cactcctaaa tatagtttgc
    42241 tgatttcagt ttatcccagt atacccattt tttcaacaaa acaagtgatt ttcttttatc
    42301 ccagcgtttt ctggataaaa aaatagatag accataacgg gcctatctat ttggtaaccg
    42361 gtgctctcct gccaactcct cggtcacaag tcacactact cataaaacat gaaaagctgt
    42421 tttttgaaat cgtttcttgt gacttagagt taaacggtag gcttacaacc tcttcattaa
    42481 acgtttttca atcagctgac cttcggtatt agctcaataa acggcaaaat atgaggagca
    42541 atttttccgt ttattgagct aatactcagg acaggacgct gatttcacaa cctctagata
    42601 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatg
//
LOCUS       DACEPW010000004        41197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEPW010000004 DACEPW010000000
VERSION     DACEPW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.004"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..423)
                     /locus_tag="IW819_000150"
     CDS             complement(<1..423)
                     /locus_tag="IW819_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1243288.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINA"
     gene            571..1712
                     /locus_tag="IW819_000151"
                     /pseudo
     CDS             571..1712
                     /locus_tag="IW819_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1838..2917)
                     /locus_tag="IW819_000152"
     CDS             complement(1838..2917)
                     /locus_tag="IW819_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1243289.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3020..3460)
                     /locus_tag="IW819_000153"
     CDS             complement(3020..3460)
                     /locus_tag="IW819_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1243290.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3517..3927)
                     /locus_tag="IW819_000154"
     CDS             complement(3517..3927)
                     /locus_tag="IW819_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1243291.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4107..4541)
                     /locus_tag="IW819_000155"
     CDS             complement(4107..4541)
                     /locus_tag="IW819_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1243292.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4641..5489)
                     /locus_tag="IW819_000156"
     CDS             complement(4641..5489)
                     /locus_tag="IW819_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1243293.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5878..7644
                     /locus_tag="IW819_000157"
     CDS             5878..7644
                     /locus_tag="IW819_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1243294.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7746..9005
                     /locus_tag="IW819_000158"
     CDS             7746..9005
                     /locus_tag="IW819_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1243295.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9152..10453
                     /locus_tag="IW819_000159"
     CDS             9152..10453
                     /locus_tag="IW819_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1243296.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10456..11307
                     /locus_tag="IW819_000160"
     CDS             10456..11307
                     /locus_tag="IW819_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1243297.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11320..12135
                     /locus_tag="IW819_000161"
     CDS             11320..12135
                     /locus_tag="IW819_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1243298.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12286..13452
                     /locus_tag="IW819_000162"
     CDS             12286..13452
                     /locus_tag="IW819_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1243299.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13682..14329
                     /locus_tag="IW819_000163"
     CDS             13682..14329
                     /locus_tag="IW819_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1243300.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14413..15357
                     /locus_tag="IW819_000164"
     CDS             14413..15357
                     /locus_tag="IW819_000164"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1243301.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15492..16943
                     /locus_tag="IW819_000165"
     CDS             15492..16943
                     /locus_tag="IW819_000165"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1243302.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16964..17800
                     /locus_tag="IW819_000166"
     CDS             16964..17800
                     /locus_tag="IW819_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1243303.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17800..18459
                     /locus_tag="IW819_000167"
     CDS             17800..18459
                     /locus_tag="IW819_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1243304.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18585..19274
                     /locus_tag="IW819_000168"
     CDS             18585..19274
                     /locus_tag="IW819_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243305.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            19280..20236
                     /locus_tag="IW819_000169"
     CDS             19280..20236
                     /locus_tag="IW819_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1243306.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            20280..20792
                     /locus_tag="IW819_000170"
     CDS             20280..20792
                     /locus_tag="IW819_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1243307.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21048..21596)
                     /locus_tag="IW819_000171"
     CDS             complement(21048..21596)
                     /locus_tag="IW819_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1243308.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21685..22866)
                     /locus_tag="IW819_000172"
     CDS             complement(21685..22866)
                     /locus_tag="IW819_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1243309.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(23102..24610)
                     /locus_tag="IW819_000173"
     CDS             complement(23102..24610)
                     /locus_tag="IW819_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1243310.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24769..24960
                     /locus_tag="IW819_000174"
     CDS             24769..24960
                     /locus_tag="IW819_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243311.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            25163..26081
                     /locus_tag="IW819_000175"
                     /pseudo
     CDS             25163..26081
                     /locus_tag="IW819_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
     gene            26074..27147
                     /locus_tag="IW819_000176"
     CDS             26074..27147
                     /locus_tag="IW819_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243312.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     KRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            27295..27813
                     /locus_tag="IW819_000177"
     CDS             27295..27813
                     /locus_tag="IW819_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243313.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27794..28186
                     /locus_tag="IW819_000178"
     CDS             27794..28186
                     /locus_tag="IW819_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1243314.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            28262..29197
                     /locus_tag="IW819_000179"
     CDS             28262..29197
                     /locus_tag="IW819_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1243315.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29330..30109
                     /locus_tag="IW819_000180"
     CDS             29330..30109
                     /locus_tag="IW819_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1243316.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            30102..30419
                     /locus_tag="IW819_000181"
     CDS             30102..30419
                     /locus_tag="IW819_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1243317.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30663..32273
                     /locus_tag="IW819_000182"
     CDS             30663..32273
                     /locus_tag="IW819_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1243318.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32430..33278
                     /locus_tag="IW819_000183"
     CDS             32430..33278
                     /locus_tag="IW819_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1243319.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            33509..33988
                     /locus_tag="IW819_000184"
     CDS             33509..33988
                     /locus_tag="IW819_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243320.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            34021..36249
                     /locus_tag="IW819_000185"
     CDS             34021..36249
                     /locus_tag="IW819_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1243321.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            36378..38021
                     /locus_tag="IW819_000186"
     CDS             36378..38021
                     /locus_tag="IW819_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1243322.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(38094..38639)
                     /locus_tag="IW819_000187"
     CDS             complement(38094..38639)
                     /locus_tag="IW819_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1243323.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(38662..39147)
                     /locus_tag="IW819_000188"
     CDS             complement(38662..39147)
                     /locus_tag="IW819_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1243324.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(39137..39949)
                     /locus_tag="IW819_000189"
     CDS             complement(39137..39949)
                     /locus_tag="IW819_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1243325.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(40088..>41197)
                     /locus_tag="IW819_000190"
     CDS             complement(40088..>41197)
                     /locus_tag="IW819_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1243326.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13681 a         7449 c         8043 g        12024 t
ORIGIN      
        1 tgcattaatc tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact
       61 gaaactgata atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac
      121 tgattcgttg cagtatcttc caaatagacg ataatagcag taagccggat caattccttc
      181 aaaattcaac tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg
      241 ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa tttgattcag acatatggaa
      301 atgatctgct aaatattgtg tagtaataaa gaaacgatgt tcagatagga aaagaaaaat
      361 atctaatttt gtattgcttt ttttcttcaa gataaacgtt ttaatcgctt cttgagtttg
      421 catgaaagag gctccttttt acttaaattg ttaaaacttg aaaatattac tgtttttttc
      481 ataaatttag acaaaaaaac agtaaacata taggtagaat ctctactatc ttatttcttg
      541 catacaaaaa aatcaaggag tgagagagtt atgagatatt caaccatctt aatatcaaca
      601 tttgcagcct taagcttatc gcttactata aatcagacag tccacacaga ggagacagaa
      661 acagccaaat ggacagaaac caagtgggcc aacgtcaatc gaattacctt tcaggatgaa
      721 ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa
      781 gcaaaaattg actattcggg agacttagca cctatactac aatcagctgt tacatttgag
      841 aatgttgatc ccactttgtc gattggctat gacgagaaca ctttgactat ccaacacaag
      901 caagcgatat ttaccttcac cgttacatta aatcaggact tgttaaaacc agcgcttttt
      961 actgtcaagg tatcagatgg actaccgaac gatcatcatt taacgccgta tagtcagcgg
     1021 cagacaattg agcaagattc agccatcgac tcaggaggag atcttgttga agaaccaaca
     1081 gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc agacggagaa
     1141 cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc agagccagac
     1201 aataaatcaa aacctgaaat aacccctgga gaaaaaccag atgtaactcc agaaccagat
     1261 acggattctg gaaaccaaac agtaccggaa acaaatccag acacagataa tgaaactgaa
     1321 aatccagaaa aaccagaggt agatccagaa gaaaaaccag atgtgactcc agaaccagat
     1381 acggacgcaa gagaccaagg aataccagaa aaaataaaca aaaaaacaat tcaagaggat
     1441 ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa aaataaatga agagcagctg
     1501 aataaaaaat cacgaatatt tgatagcgcc caatcagctg aaaccttaaa aagtagcaag
     1561 gatacaacct ttgctgtcct gaaacgaaaa ataaacagct accaaaatcc ggagagtcac
     1621 aaaacaaagt tatactgtgg agcggaatca ttctattaag tatagcaaca atgctttctg
     1681 caaaaagatt taaacaaaac agatctctct aacatttgaa taatgtctgt gaacgcattt
     1741 ttcaaataaa aagaaatatc cagaaaatga ccccaaaata ccagattgaa atctgatatt
     1801 tggggtcgtt catactttat ctttcttcat atttatttta agggatatct gtttcttttg
     1861 gaagatggtt cgggatcttt ttcactttca atacatctaa aaagaaggta aagatcggaa
     1921 tgccgacaat caagccccaa acgccaaaga accgttcgct aacaagtaag accacaaatg
     1981 tataaaaaat cggcagttct gttttgctgg acatcaactt cggattcagc acataagact
     2041 caattagatg gacaatcaaa atcgtaatca ggatataaac aacatctcgg atgcctcctt
     2101 gggaataagc tatgaaagat agaggaatac aagaaatgat aacacctgct actgggatca
     2161 gacttaaaat gaaaatcatg atcgctaaac tcagcagctg atgaaagcca aatacagcca
     2221 aggcgattgt agtgagcaac gtatttaaaa gagcgacaac aaattgagct tctaatacca
     2281 aaccaaatgt attgacaaac ttatcggcaa aatagtaaat atcttgaaag aaccaagaaa
     2341 attcactttt taaaaacaat cttgagaata caattgtttt tttcttttca atcataaaaa
     2401 agaaacttaa gatgaatgac atagcaaacg ccactgccat ttttcctata tcctgtaaat
     2461 agccaagcgc aatcgaagcg ccattttgaa gctgttcaaa aaaatcgttt cgctcaatat
     2521 aagtatggat atattgtaaa acaggatctg catcttttgg ctgatgctcg taaaaattaa
     2581 taacggactg gatcatgtca aatgtttgat gaataagcat cgggatatac tttgtgaatg
     2641 ccagatagat aaggtaaacg atcagtgtat aaatcatgat agctaaaaca cctgtaggga
     2701 ttcggacaaa tcgctgaatg atttgaacaa gatgaatcat caaataagta aagataaatg
     2761 tgagcaaaat ggttgtaatc atacttctaa ctaagaaaag aacaaaaacg acaaataata
     2821 aaacagtgag acggcgtatt ttttcattcg ccaaaaaacg ctgataaacg gtcaaatcga
     2881 caacctccaa ataacaaaaa ataaacacgt tattcattat atgatggaat atgaaaataa
     2941 gaaagagcaa aaagaaaaac cgctaaatga cgttatctta tgagttggtt ttcctcataa
     3001 aatcgttcat ctagcagttt cagccctttt tctggtcttg atgaaaatta tctagcattt
     3061 ttcgggtcag atgttgcagt gtatccaatt cttcctgttc aagtggaaga taagacttga
     3121 tcttttgtgg aataccttct gcttgttttt gcaacgcagc accttgagca gtcagcgtaa
     3181 catttacgat tctttcatca cccgtttcac gctgacgatt aatataattt ttttctgcta
     3241 acttttttag taagggagtc agcgtaccag aatccagaca caaatcattt cctagctctt
     3301 tcactgaagt ctttttcttt tcccataaaa ctaacatcac aagatactgg gtataggtaa
     3361 gattcagcgg cttgaggagg ggagtgtagt gtctaatgat ttctttagag gcggcatata
     3421 aaggaaaaca tacttgatta actaaatata gatcttccat ttttccacct ccgctgtttt
     3481 actatagtgt atcatagcgg atttacacaa aaaagtctat tcactaactg ctttcagagt
     3541 aacttctata tttccagctg tagcttttga atacggacac actttatgcg ctgcctctaa
     3601 taattcttgc gtttcttcca gagaaagacc ttcgatagat ccttcaatct ctacacctaa
     3661 gacaaagctc gtttcacttt ctgaataaag ggaaacttgt gcactgacag tagaagcccc
     3721 agagatattt ttttgctgtt tgatcaattc taatgcactg ttaaaacatg cactgaaacc
     3781 agctgcaaac aattgttcag ggttggttgc attttcgacg cgtttaccag gagctgcaat
     3841 attcaattcg aatgtacgat cgggattatg cacttctcca gagcgtccgc ctgtgttgat
     3901 cattttcgta gaatacattt ttttcataaa attaccacct ttaattatat tttatacaat
     3961 tgaattttac acaatataat tgtggaaagc aaagaaatgg attggcataa gataaataag
     4021 agaaagcaca tgaaacagac gcgctttctc gaatagcaaa catatacaaa aaatatcaga
     4081 gacaaaaaat aatctcatca taaacattac atatatttct caaaaagttg ttttctttct
     4141 tcaaaaatta cttcacgatg gacctgatga agcatccata aataaccaaa aggatctctg
     4201 aacattgagt tagtcacacc aaagtccatc atttctgtca tttcttgaat ctctacacag
     4261 ccatttcgta atgctttatt atgtgtgtcc tcgatatcag agacgatgat attgaaccag
     4321 atcgtatttg gatgttcagc agtaggagca gtcaaaccaa gagacgtgtt ttcatccagc
     4381 atatggaatc tcgtaccata caaggtaaaa atgacttcat tattgcctct agggtaatct
     4441 gttgcctcaa tcagttgtat ttcatcaaag attgtttgat acagttctag tgctttcaaa
     4501 ctatcattta atacaaaatc gatttccgtt cctttatgca taaagatctc ctctcctttt
     4561 cagtcgaaca gttactagat tcttttatta taccagctaa agaaaacgca ttcaaaaaaa
     4621 tagcggattt acttaccaac tcattcgatt ggatgataag taaatccgcg tcccattact
     4681 tctgctacgc tgctgacaat gacaaaggca cttggatcga tttcttctat caatggtttc
     4741 attcgggaaa attcatgatc agtcacaacg atcaataaaa cctcttttga ctttcgatta
     4801 tacccgcctt ttgcctctag cagggtaagg ccacgtccaa tctcagcact taagcgaagg
     4861 cggatgtctt ccaaataatg ttcactcatg accataacag atttctttcg attgatacct
     4921 gtttcgatat aagtcatgac aattgaagtg attgcaatag ataaaatagc ataaagaaac
     4981 tcttctattc caaaaataaa cagattaaga gaaacaacga atgcgtctgt aaaaaataag
     5041 ccaatcgaag tattcaagtg gaaatatttt ttaaaaatga ggggagggat cgtcgtaccg
     5101 ccgctagaag agttgttttt atataaaatc gcaactccta aagcaaaaat cgcacttcca
     5161 aaaatgacag acaacaaccg atctgaagta atcattgttt ctggaactat tgcaatgatt
     5221 aaaggaaaaa ccaagcttcc gaaaagtact ttaaaaaagg gttttttacc taaaaataga
     5281 agagctagta ccaacataac gatattcaac actaaaataa ctatagctcg gtcaaagcct
     5341 aaagcagctt ccagtaatat tcctagaccg ctgacacctc cagcagcaat atgatgaggc
     5401 cctaaaaaca tattgatagc gactcctaat atgaagaggg caaaaataat cttaatgtat
     5461 gtccatataa attgtctcgt ttctttcatt tattcacttc cttaattcat taataatact
     5521 cttatcttat tataacttac ttttcacctt aagttaactt taattaataa aaaaattagg
     5581 ctagtctaaa ttattatgtt tcattcttct agaatatact tctacgccaa aaactgttaa
     5641 aaaactttta ctttagaaaa ttggagaaat tgatcgctta agataaattc aggacactaa
     5701 atagtacagg aagtagacta aaacttttaa aagttttaag tctatctatt tggcaagttt
     5761 aaaaaataaa aattcggtat caaaaaactg ttaccgataa catttttgaa agcgttgtct
     5821 tttgcttctc tatcacttat agtgatgacg tcatattaaa acacaaggag gaaatcaatg
     5881 gatacagctg caatttatca tcgaccggaa agtgaatatg catttctata tacgaataca
     5941 agatttcgta ttcggcttcg tactaggaaa aatgatatca aaaaagtata tgtgctatgt
     6001 ggagatccat ataccataac aactgaaaaa tggtatcaaa aacaacgacc aatgaaaaaa
     6061 tgtctaagta ccaatgttca tgattattgg gaaattgaag tgacgagcga aacaagtcgc
     6121 ttacagtatg cgttccatgt tgttggggaa gataacacgg actgttttta cggtgatcaa
     6181 ggaatttttc cttatcgaga aaatgtgatt actgaaccaa atttttattt tcgaatacct
     6241 tattttcatc aaatcgatcg attcactatt ccagaatggg taaaagaaac agtatggtat
     6301 caaatttttc ccgaaagatt tgcaaacggg gacaaagcaa atgaccccga acacacttta
     6361 ccttggggaa gtaaagatcc agaccgagaa gactttttcg ggggagattt gcaaggtgtg
     6421 atggatcatt tggattactt gacggacctt ggcatcaatg gcatctattt ttgtcccata
     6481 tttaaagcca catccaatca taaatacgat acgattgatt attatgaggt ggatcccgct
     6541 tttggcgata aaaaactttt aaaaaacttg attgatgaag cacataaaag agggatacgt
     6601 atcatgttcg atgctgtatt caatcatatg ggtgtccatt caccacagtg gcaagacgtt
     6661 ctcaaaaacg gagaaaaatc gatctataaa gactggttcc acatccgttt tttcccagta
     6721 gattcttatc agatgactga cttaccagaa actgctgaaa atatcccata tgatactttt
     6781 gcttttactc cattcatgcc gaaactaaat acagcaaatc cagaagtgca aaaatatttg
     6841 ctggatatcg caacttattg gataaaagaa ttcgatattg atggatggcg tcttgatgtt
     6901 gctaatgaag tcgatcatca tttttggaaa aaattcagag aagcagttac agaaatcaaa
     6961 ccagacatct atatccttgg agagatttgg cattcctctc aagcttggct gcaaggggat
     7021 gaattccatg cagtgatgaa ttatgcattt acagattcga ttaaagatta ttttgcaaaa
     7081 aagaaaatca cagcttcaca aatggtcagt ggaatgaatc atcagcaaat gctttacaga
     7141 gatcaggtga acgaaggaac attcaactta ttagattctc atgatactgc caggatcttg
     7201 accctttgcc aaggtaacaa agaattgatg aagtctgtga tggcctttat gtttcttcaa
     7261 aaagggtctc cttgtatcta ttacgggaca gaaataggga tgactggtga agatgacccg
     7321 gattgccgca aatgtatgat atgggagaag gaggaacaag atctagagct attcggtttt
     7381 ataaaagaac ttgtttctct aaggaaacag ttgtctaaga ttatttctga aggttctact
     7441 cagtggctaa tagtaaatga tcgtgaagat aagctctatt tcacacggaa actagaggga
     7501 cagatcatct atgtttactt taatcagtca aaagaacctt gggtagtaga acaggaaaat
     7561 gaggtgattc ttagtcaaaa ttgtcagttg ttagaagacg ggaaagcaga gatacgacaa
     7621 tacggatttt tgatcatgtg ttgaaaactg ttaccggtaa caacaagaaa gcgtttgcaa
     7681 atgaagattt gtgcccatta taataagggt gtaacagcac gttgaataca aaggaggaaa
     7741 tgaagatgaa aaaaagaagt gtgaaacagt tgggtttagg gttaatctct ttaggaatct
     7801 taggcggatt agcggcttgt ggcaatggta actcttcagc agaaggggaa gccgatgaag
     7861 gaaaaacgtt aacagtttcc gttgattctg gatataaaga ttatataaac gaaataaaaa
     7921 acactttcga aaaagaaaac gatgtgaaaa tcaagctagt agaaaaggac atgttcgatc
     7981 agttggaatc attagcgtta gatgggcctg ctggaaaggg acctgacgtt atgatggcag
     8041 catatgaccg gatcggtgcg ttaggacagc aaggacatct ggcggaagta aaactgggaa
     8101 atgaagcagc ttacgatgaa acagacaaag cccaagtgac ttatgatgga aaaatctacg
     8161 gagaacctgc agtaatcgag acccttgttt tgtactataa caaagattta gtcgatacag
     8221 caccatcaac gtttaaagat ttggagaatc tatccaaaga cagtcgtttt gcttttgagt
     8281 cagaagcagg aaaaaataca ggatttttag ctaaatggac tgacttttat tattcctatg
     8341 gtttaattgc aggttacggt ggctatgtat ttggtgatga tggcacaaat ccttcagata
     8401 tcggtttgaa taatgcaggt gctgtagaag gaatctctta tgcaacagat tggttcaaaa
     8461 atgtttggcc aaagggaatg caagacataa aaagtgcagg agacttcgcc agccagcaat
     8521 tcatgtccaa taaaactgca gcaatcattg acggtccatg gcaagcgcaa acatacaaag
     8581 aaaacaatat caattatggt gtagccaaaa ttcctactct aaataatgga cagccttatc
     8641 aaccgtttgg cggaggaaaa ggatgggtag tcagcaatta tgctaaaaac aaagacctct
     8701 ctcaaaaatg gcttgattat gtaacgaatc aggaaaatca agaaaaattc tttgaaatgg
     8761 taaatgaaat ccctgcaaac caacaagcta gggaaacagc aaaggctaaa aacgatgaat
     8821 taacaacagc tgtgattgaa caatatgaaa cagctcaagc gatgccaaac atcccagaaa
     8881 tgggcgaagt ttggacaggt gcagaaaact tgatgttcga tgcagcatca ggttcaaaaa
     8941 caccaaaaga atccgcagat gaagcagtaa aaacaatcag tgaagccata gaacaaaaat
     9001 actgattaaa atgataaccg tccattttta taaaaaatgg aacaggtgct tcagataaaa
     9061 gtggcaagag cgagatggaa aattatcttg ctcttgttgc cattatctta aaagcagttt
     9121 gcggtttagc aagtttagag ggggcattca gatgcagcaa gataaaacaa gcaatatcaa
     9181 cgaaaaaaaa atcacgcttt gttcacttat tccagggatg gggcagtttg taaatggcca
     9241 agcttttaaa ggtatcgttt ttttcgccat attcgtccta ttcattggtc agatgcttct
     9301 gggcggattt catgcattgg taggattggt tactttggga agtataccaa tggaagatca
     9361 ttcattattt attttgattg aaggcacttt gcaattcatc attacaatca ttttcttgat
     9421 tttttacggt ttgaatcttt tagacgctta tcgagtagcg aagatgcgtc aagagggcaa
     9481 aaaaatcaat cggacgatga aggaagtcat acataatata ggtgatgaag gttttcctta
     9541 tctattgaca cttcccgctt atcttctcat gatttttacg atcattttcc cagtcctagt
     9601 tacattgttc actgcattta ctaattatga cttcaatcat attccaccag ccagtttaat
     9661 tgattgggta ggggctaaaa acttttttag tatcttcttt ttaagctcat atcgtaatac
     9721 tttttttgca gtcttttcgt ggaccgtgat ttggacttta tgtgcaacaa ctttgcaaat
     9781 cacccttggt attctcacag cggttgttgc tcaccagaaa tttattaaat tcaaacgtat
     9841 tttcggtatc attttcttat taccatgggc ggtcccagct tttattacta tcatgagttt
     9901 ttcgaatata ttcaacgata gcgtaggtgc catcaatact caagtgattc ctttcataaa
     9961 ccagcttcct tttgtagata tcagtgcaat ctcttggaaa acggacccga actggacgaa
    10021 agtagcaatt atcttagtgc agggctggtt aggatttccc tatatctatg tgatggttac
    10081 aggaatctta caatcgattt cagaagactt atatgaagca gcaaaaatcg acggagcaaa
    10141 tgcatggcag cgcttttcaa acatcacgct tcctgctgtc ttcctagtgg cagcgccaac
    10201 atttattaca cagtatacgg ggaatttcaa taatttctcc atgatctatc tgtttaacaa
    10261 tggcggtccc ggaagcgtag gcggaggagc tggttctact gatattctta tttcctggat
    10321 ctataaactt acgactggaa cttctccgca gtattctatg gcttcagcaa tcacgttgat
    10381 tatctccgtc atcgttatct gtgtgtcatt gctggtattt aaaaagacaa atgcgttcaa
    10441 tatgggggat taacaatgaa aaaatttcat tcgagtgctc gattccaaaa acgactttct
    10501 cactttttta cttacttttt tcttttaata ctgtcagtca ttatcatcta tccgcttttg
    10561 atcacagcaa gtactgcctt caagtcagga aatattgcag cttttagttt agatctttca
    10621 agtgactgga ctttaggaaa ctttactcgg ctattcagtg aaacacttta cgggacttgg
    10681 tataaaaata cattgatcat ttctgtccta acaatgatcg tacaagtgac tgtagtaact
    10741 ttggcgggct atacttatag tcgctatcgt tttatcggcc gcaaatcaag tttgatgttc
    10801 tttttagtga ttcagatggt accgactatg gcagcattga ctgcctttta tgtcatggct
    10861 ttcttattag aagcgctcga taagtactgg ttcttgacaa tgatctatat aggcggtgga
    10921 attccaatga atacttggct gatgaaagga tactttgaca ctgtaccaat agatttagat
    10981 gaatctgcta aacttgatgg ggcagggcat ttcaggattt ttttccagat tgtattaccg
    11041 cttgtcaaac cgatgctcgc cgttcaagca ttatgggctt ttatgggacc ttttggggat
    11101 ttcatgttag ctaagttcct gcttcgaaca cctgaaaatc agactgttgc tgtcggctta
    11161 caaacattta tttctgatgt gacaaaccaa agagtctctc tttttgctgc tggggcaatt
    11221 ttgattgcac taccgatttg tctattattc ttctttttac aaaagaactt tgttaccggc
    11281 ctgacatctg gcggaacaaa aggataggag cagagtatca tgcaaacatt tccgttaaac
    11341 tatttttccg cattacgtac gccaactcaa gtgtttgcgg gacgcaaact attatcatgg
    11401 cctaaatttt tcttgatatt tgtattttta gtttccttga tggtcatgcc tgtgactctt
    11461 ttttatgcca atcagatcca agcgattcca ttagaacaat ttctatccgt tcattcgctg
    11521 atcgatgaac aaggaaccca aaaattttct gaacttgaat tatctgaaac tgggttacaa
    11581 gcatcgcaac aaacgatagc tgtgacccct gaaattttgg taggtgtgtc tttatctgag
    11641 aaacaacaat ccgatcatgg aacttttatt gattttgaga aggaacaatg ggtgatccaa
    11701 caaaaggata aatctggtat acgccgctat acgatgaatt atagcccatc tttccaacca
    11761 gattctgtac gtacgcccga agatttccaa cgatttttag aaagagaatt ttatgccagt
    11821 aatcgtccaa caatcatttt atcttatagt ttaagtcttg gtcttgtcct atttgtcatg
    11881 acgagtctca ttttattcgg ggcttccttt ttcttgtgga tgacaagaaa aagccaactt
    11941 tcatccattc atacattcaa agagagtgca aatttgatgc tgaatgttat gggaataggt
    12001 tccatggcag cagcagttgt agggtttatc cactttgact ttatcttgat gctaagtgtc
    12061 caaacaacga taactgtctt gctgctttta tgggtttttg tcaaaacaag attcaaagat
    12121 aagcgtgtcg aatgacaagt ttaagcgatt tatgcgacaa tgaatgtgaa aaaaatgaaa
    12181 aataaagtag tttttgaaaa atttctgaaa aaattaaact tcttagttga ttcttttctt
    12241 ttttagtatt aacgtaatag ggtagttttt ttaaggagga aaatcatgtt agagaaaact
    12301 ttatacaatc aattattttc acgttctttc tcgctccctg tcgaagtaaa ttattgggac
    12361 ggtacaacga aacaatacgg agatacggac actcctcccc aaatcaaaat caatatccat
    12421 gaagaaatcc caatgaaaga gattacaagt aacgcatctt tggcactagg agaagcctat
    12481 atggatcatc gaatcgagat cgagggaagt atccaagcat tgatcaacga tgcttatcag
    12541 caagcaaaca gctttttacg aggaaaagat tatctaaaat ggctgccaaa gaaagaaaaa
    12601 catacgaaac aaaaaaacaa agaagatatc catgcgcatt atgacttagg taacgacttt
    12661 tataaacttt ggctagatcc aactttgact tattcttgtg cttatttcaa tacacctgaa
    12721 gatacgttgg aacaagcaca aatcaataaa gtccatcata tcttagacaa actctttatc
    12781 aaagaaggcg aaacgttgct tgatatcggt tgtggctggg gaacattgat gttcactgct
    12841 accaaagagt ataatgtcaa ggcaactggg atcacactaa gtgaagaaca atacgatttg
    12901 attaccaaaa gaataaaaga agaacattta gaagataaat gccgtgtatt attaatggat
    12961 taccgcgaac taaaaggcga aacgtttgat catatcacaa gtgtaggtat gttcgaacat
    13021 gtaggttctg aaaatcttga aggttatttc aaagtggtaa aagatttatt gaaaccaaaa
    13081 ggaacagctt tgatccatgg aatcagccga caacaaggcg gtgcgacaaa tgcttggatc
    13141 aataaatata ttttccctgg cggttatgtc cctggtgtta ctgaattagt tgataatatt
    13201 acaaaaaacg atttgcaact gattgatttg gaaagcttac gtcgtgacta ccaattgaca
    13261 ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc aagtcacgaa agaaatgggt
    13321 gattccttct atcgaatgtg ggatctatat ttacaagctt gtgcagcttc atttgaatca
    13381 agtaatatcg atgtgattca atatttgctt gttcaacctg gaaacaatga catcccaatg
    13441 catcgtgcat aattgaggca aaagtactgt ttaaagacta tttaaaaaga agctaagttt
    13501 tatgatgctc cagaagtgaa cttttttgtc taacttctag gattgtctct aaagcttagt
    13561 ttctttttct ttatattttc ataataaaag tttggttctt taatttttaa acatgacaga
    13621 aaattttttc ttcgttatac ttgtaataga atactatttt ataacaaagg ggatcgaatc
    13681 aatgaaaaaa actgttattt atactagttt atttgcagct ttgtttttag ctgcttgtca
    13741 atcaaatacc aatcaaggca catctgggaa tacgtcttcc agtagcgaac agcgctcttc
    13801 acaggtatcg gactcctcaa caaaagcaag cacatcttcg tctagccaga caaaaagttc
    13861 tagtgaagca agtactcagt cagatatcaa agtgtctgca gctcgtgcga tcgaattgtt
    13921 ccaagataaa tacccagata ctgcaattac tagtttggaa ttagatagtg attggggatc
    13981 ttacttctac aagatagaag gtgtcgatga tcaaaaagaa tacgaagcaa gaattgatgc
    14041 tcaaaatgaa aaaatggaga cagagaatcc agaaactcta gatcgagatg aacaaaatgg
    14101 tcaaaagaaa aaagaagatg gtctggatgt tgataaattg atcacgattg aggaagcagc
    14161 aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca gattgggatt tggataaaga
    14221 tttaggtact acttattggg aagtcaaagt aaaagacggc aatcaaagta catcagtcaa
    14281 gatcaatagc caaacaggcg aagtactgtc aactgaacaa gatgattaag aatttcttcg
    14341 gaaaacaaga gttgattttt cttttagtca tgatacacta gacaaaataa cttgtttgaa
    14401 ggagcgattt acttgagttt agaaacgacc tttgccaatc atatctttgc caatcctttg
    14461 atgaatgcat ctggtgtcca ctgcatgact acccaggaat tagatgaact ggctcattca
    14521 gaagcaggag catttatcac taagagctgt acgatcaatg aacgaaaagg aaatcccgaa
    14581 ccacgctatt ttgatgttcc tttagggagt atcaattcta tgggacttcc aaacttaggt
    14641 ttttcttatt atctagagta tgctcttgcg tatgaaaaag tgcaggaaaa ccaaaatcaa
    14701 ccgttatttt tttccattgc cgggatgagt gtccaggaaa acttggaaat gcttgagaaa
    14761 atagaaaaaa gtgggtttaa tgggatcact gaattaaatt tatcttgtcc caatgtgcct
    14821 ggaaaaccac aactagctta cgattttgag gccacttatg aaacactgaa agaagtattc
    14881 agcatctttt ctaagccgct aggaatcaaa ttgcctcctt attttgattt tgctcatttt
    14941 gatcagatgg ctgatatctt gaatcagttt ccattgacct atgtcaatgc tatcaacagt
    15001 gtgggcaatg gtttgtatat cgatacagaa caagaagcag tagtcatcaa accaaaagaa
    15061 gggttcggcg gcattggcgg agagtatatc aagccgactg ctttggcgaa tgttcgcgct
    15121 ttttatacac gtttgaaacc agaaatccaa atcattggta caggtgggat tcggacagga
    15181 caagatgcat ttgaacatct gctatgtggc gcaagcatgt tacagattgg taccgagctt
    15241 cataaagaag ggccggaaat tttttctaga atcataaaag aattaacgca gattatgtca
    15301 gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat tacgcaccat ttcatagcaa
    15361 agagacgaag gtcatgattg atcttcgtct ttttttataa aaaacactcg tctgttcatt
    15421 cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg ggaaaataac agaggcaaaa
    15481 aggaggataa tatgaatgat gcaacagaat tagcaaaaga gcttaaagaa aaagaattta
    15541 gttttcaaga actagttacg gatatacaga aaaaaataaa gaagcaaaat catgaattga
    15601 atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca cgagctattg attccccagt
    15661 cagaaaaagg gccattggct ggtatcccat ttcctttaaa aatgctcggt caagaaaaaa
    15721 agggatggct agctacttca ggatctcgtt tatttgaaac acaccgagct tctcatacat
    15781 ctaattatgt gcggcaagca gaagcgatag gtctagttcc ttttggacaa accaatgcgc
    15841 cagagtttgg tttcaaaaat attacagacc cagtcatcta tggcccagca agaaaccctt
    15901 ggaaccttga atgtacaccg ggcggatcaa gtggaggagc tgctgctgca gtttcatcag
    15961 gaatcgttcc tttagctggt gctagtgatg gcggtggttc tattagaata cccgcttcgt
    16021 tttgcggatt gattgggctg aaaccttcgc gagggacgat gccagttggt ccatatgctt
    16081 ggagaggatg gcaaggagct gctattgatt tcggcttgac agtttctatg agagacacgg
    16141 aggctttatt ttatgggatg agaagtatcc attccggtgc gccatatcaa gtatcgccag
    16201 ttgagtggca aacacatcca agaaaaaaac ggttgaagat agctttttgt gttgattcac
    16261 cgatccactc acagattagt gaagaggcag tacttgctgt aaaaaaagct gtagatttcc
    16321 ttggaaaaga agggcatgat ttgacagaga ttccttaccc actagatggt cgtaaactga
    16381 tcacgtctta ttatcagatg aatggcgcag aaacagcagc aatgatccgc tcgattgaaa
    16441 caggcatcca gcgagctgtc agaaaagaag aacttgaacc catttcttgg actttgttcc
    16501 aatatggtca gaagctctct gccgctactt acatcgattc tctgcatgta tgggatgaag
    16561 cagcaattgt catggaaaac ctacatcaag aatacgatct attcctttca ccaacgactg
    16621 catttaccgc ccctaaaatc agcgaagatt tgcaaagtga tgcgataaga gcacagatgg
    16681 aacacatcag tgattgttct gaagtagaag gtttagacgt tgtttcttcc atgtttgagg
    16741 agagcttgaa gatcacacct tatacacagt tggctaatct gactggacaa ccagcaatca
    16801 gcttgcctac tcatttgaca aaagaaaaca tgcctctagg aatccaattt atggctgcca
    16861 gaggaagaga agatttgcta tttcaaatcg gctatctatt tgaagcaaat catcagttcc
    16921 atttaccaaa tccatggaaa tagcagaaac aaggagaaga agaatgaaga aaaaaagatt
    16981 gtttgctcta ataactagtg ccttttttat tttaagcgct tgcggtacag ctgccgctaa
    17041 tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa gaattagtag tagcgacttc
    17101 tggcacactg tttcccgcat cttattataa tgatcaaaat gaattagttg gttatgatgt
    17161 agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa atctccttta aagaatacaa
    17221 cgtggatgga caaatcactt ctgtttcaaa aagagaatca gattttgctg cgaacgattt
    17281 tggtatcaca aaagaaagag aaaaaaaatt ctccctttca gagactttca aatattcatt
    17341 tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc cactcacttg aagatcttaa
    17401 agggaaaaaa gcagctggcg aacccaatac tagctatatg cgtttagcgg aaaaacacgg
    17461 agctaaatta gtcacatatg acaatgccac gaatgatcag tatttaagcg atgtagcaaa
    17521 tggtcgaaca gatgttattc taaatgatta ttatctgcag aaaatggccg tatctgcttt
    17581 accagatatc cctgtaaaaa tactggagaa cgtttatttc aatgaaaacg aaagtggttt
    17641 tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc gatggcgtat taaaagagat
    17701 gaaagaagat gggacgacaa agaaaatatc tgaaaaatat tttcaaactg atgtttcagt
    17761 caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga tgtactttcc tctaattgat
    17821 ttttccctca tgtggacttc acttcctttc gtactgcaag gcttggtata cacattaggg
    17881 attggcttcg tgtcgtttct tttaggaaat ctggtagggt tgcttttgac agttttaggt
    17941 cttttggatt ggctgcctct gaatgtattt attcgttttt atttatcgtt ctttagaggc
    18001 attcccgcac tagtcctgct gtttctgtta tattttgggt taccttatca attaagtgcc
    18061 ttaacggctt cagtcatctg ctttacgatt accagcagtg catttattgg tgaaatctat
    18121 cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg ctggtttagc ttctggtttt
    18181 tctttctttg gggtattgaa atatatcgtt ttgccgcaag cttttcgaat ctcagtaccg
    18241 gcactcagta acgtagctat ggatctagtg aaaggtactt cgttagcagc aacaatcaca
    18301 gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc ggacattcga ttatttttct
    18361 ctctatattt tagttgcact gatttattgg gtcatttgta tcgggatcga aaaagtgcaa
    18421 cagtatttag aaaaacgttc tatgcatcag tcaatctgaa aaatagaaat ggtgctagcg
    18481 tggctggcac catttctatt ttttgtttct tgtatattct gcctagtatt ttaaaaccat
    18541 ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga aaacatgaag accccgctaa
    18601 ccatcgaaat agaatcctcg ttataccaat attgcatcga gcaaggcggc ttggttgtag
    18661 aagaagtcac gatgcctgat gaccaaggaa ttgtagatac gcttagctgc ttgacaaaat
    18721 ttgatggaag ccgagaatgg cgatgctatg agctgaaagt gacaaaagca gattttcgct
    18781 ctacagccaa actgtcattt gttggacatt ataattattt tgttttacca agtaaactat
    18841 atgaagaagt aaaaactgaa atccctacag gaataggtgt tttagtgtat cgtccgtaca
    18901 cggttgaaga ggagatgcct gcctctggta catttctgat tgcgaaaaaa gcagcaaaga
    18961 aagagcttgc tgtaccggaa gaagctttga caaaccgctt catggcttct ttattcaggg
    19021 aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata ttttccaaca gatcaattgt
    19081 acaaagaatt gaaaagacgt tcagaaaatc aagatccatt ttctaacgaa cgatattatg
    19141 aacggttttt agaggaaata gctagtcagg caatcaatga gttgcaagaa gaagttatcg
    19201 cgcttcagca tgattatgaa tatttgaggc aacaacagca gatccgtcgg ttgcctacag
    19261 agccattgga gtgaaaaata tgtactatcc ttatttaaga ggaaaacaat ttgacctatt
    19321 agcattaaaa gaagcactgt cgcgaggatt gctctccaat aaaatccaac cagtgatcga
    19381 accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa ttatttcaaa aaaagcacca
    19441 tcgaatcgct gtgatccaaa atccccaagt aggacaattc aaattattcg atcagcatgt
    19501 gcacagttgg acagtcaaag aaaatagctc tgtcgtccct gcacagatct taacgccaga
    19561 aaatctcagt gaagtactgg attcacctcc agcgtttctt gttttcgatg gacagcacta
    19621 cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt gattcgaaat ttttgatacc
    19681 agatactagt cgctttcgca tctggctgcc cgaaaacaaa attgtgatcc gtgactcttt
    19741 tcaaacgaga aagcatgttg aaagttatgc agataaaaca gatgactttt ttagtgatga
    19801 ctatcttttt ttccacgcag acggatatat tggattttct gatttcacga tcgaaggtag
    19861 ccgttatttc gataaagggt tccctagccg agcgcttgct atccatctaa cgtatattga
    19921 tgcttacggc aatatacgcg tgaaacattt tgtctctgac agtaacgact ctgctaaaga
    19981 ccaagcattg aagtttcttg aagcaggaga taaaatgaaa aaatggatca tgcgtcacca
    20041 tcatcaactc ttgattactt caggaatgat tgaattattg gctctttacc agcaacaaaa
    20101 attccctgga ctgggagtgt tgaaaaagtg gtcacttatg catcatttgg aacttattag
    20161 tcacctgcta gatcatccaa aagattggct taaatattat tcaaaagttc aggaactata
    20221 tgaagtgatc caatgattag ataatgtaaa tgaaaaagga atagaaaaga ggaaactgga
    20281 tgtatattcg gcaaacaagg aaagaagaag tagaagaagt ggtagaaatc attaatgccg
    20341 cagcggattt cattgcttcg caaggctctc ctcaatggca aaacgggcag aaaccaacaa
    20401 aagaaacgat tttgaacgat attgaaagaa aagaaagcta tgtattaata gtggaagatc
    20461 aaattgctgg aacagctgca ttagtctcaa gcgtggatcc cgtttacaca aaaatagatg
    20521 gtagttggaa agaatcaact ggtccttatt tatcgattca tcgtgtcgct ttagcagaaa
    20581 gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca tttgttgacg attgcagcca
    20641 gcaaaaatat ccaagatatc cgtatcgata cttatccagc taatgaaccg atggaaaaga
    20701 ccatctattc tgctggattc acttatcaag ggatgattga atttcctttt gaacacggcg
    20761 aaagaaaagc atatcaaata atcatcaatt gactataaaa tcatctgagc aaagataaag
    20821 acattaacac tactgaaaaa aacaatcata tgatagccaa gaggttgtaa cagaagtggc
    20881 cagcttcaaa aaacaagaaa gaatttccga aaatgcttct caaatttttg tggaatttta
    20941 gcgtatttta cgaaatagct gctttctgaa actcccttca tcgagaggtc gtgacatagc
    21001 ttttgtcacg acctctcggc ttatcctacg agtattattc gatacattta tatcaataat
    21061 ccttttttta ttttatctgc agcttcttgg aacgctccat cgtttgacac gactaaaagt
    21121 gaggggatag ctttcaaacg tttcaactgt gctaaatctt ctacataaac cgccagtctg
    21181 gctgaaatct gttcagatga atctccacgt tttttcaggc gcgattgcaa tgtttcttta
    21241 gagatcgtaa taaagatagg acggactacc gtcccgaaat actcatataa atgccaaaaa
    21301 ccatttgctg tcaaaacggc gtaacaattt cctactgtca gtttcttttt aatttctgct
    21361 ttactggaac catatttatg ttgagcatat tggtcccact ctgcaaactc atttcccttg
    21421 atcatctgat tgaatgtatt gtcagaaaca aaataataat cctcttgatc tttttcccca
    21481 ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt ccggctgaaa aatagcttct
    21541 gcgagtgaag tttttccaga accagaagga ccgataaata catagcaata attcattgtc
    21601 atttctcctt tgatgattga acaagtgaaa aagctttgga aaatatttcc aaagctttta
    21661 aaagtaactt tgcttttttg tatattatac cagcaagcca gtcacaatag cacttaaaaa
    21721 gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt tttgcgacaa gatttccttt
    21781 ttcttcattt aatcccttca tcgcaccaga aatgatcccg atagaagaga agttggcaaa
    21841 tgaaacgagg aagacagata taattgctgt tgtacgctca gtaaaatgga aattaccatt
    21901 tgctaattct gtcatagcaa caaattcatt tgtaaccagt ttagtcgcca tgatactacc
    21961 ggcatccact gcttctttcc atgggattcc gctgatgaag gcaagagggg caaagatgta
    22021 acctaggatc tcttggaaag tgatgccgaa tatcccgtgg aagatagcat tgatcatggc
    22081 aatcaaagca acaaaaccga taagcatagc agcaaccgtg atcgccacat ggaaaccatc
    22141 aagaatgtat tctcccaaca tttggaaaaa tgtctgttct ttgttttctt caatgatcag
    22201 ctcatcttct ttttcttcta atacataggg atttacaatt gaagccagaa tataaccgcc
    22261 aaataagttc aaaacaaggg cagtgatcac atatttagaa tcgatcagtg ccatataaga
    22321 tccgacgatc gacatagaaa ctgtagacat agccgataca ctcatggtga acaaacgttt
    22381 ctcagatagg aagggtaatt ccttttttat ggaaataaaa acttccgatt gtcctaaaat
    22441 agcagaagct actccattat aagattccaa tttgcccatc ccgttgattt tacttaatcc
    22501 tagaccaaca gctttcataa ataatggaag aatcttgata tagcgtagaa taccgataat
    22561 agcagaaata aatacaattg gcatcaatac ggaaagaaag aaaggtgcac ttcctttatt
    22621 tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca aaagcaagaa gatgatcaaa
    22681 catttttgcc aatacagaaa caaccgctgt accaaaagtt gtacgtaata atacgaaacc
    22741 aagaacaagc tgtaaaacga acatcacaat tagtggtttc aatttgattg cttttttgtt
    22801 actgctgatc aaaaaagcaa gccctaatgt gacaaataaa ccaataatac ctaataaata
    22861 actcaaataa tcactcctat tccaaattcc gttttatttt acctgttttg caaataaaat
    22921 actaatcttt agtgaaattt tcattacttt tttagagtga aacgcattca ttttccatta
    22981 gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa cgtcttagta aagtctataa
    23041 aaaaacgaaa ctatcgatta agctaatagt ttcgttttca acatttataa gtaaactttt
    23101 tctattggta atatagattt tcggaagggt agacaggtgc gcaggtaata tcgattccta
    23161 gttttttcaa tgtttgttca tcatttttat tgaccatcac agtagagtgg gcttgtgctc
    23221 cctcaagttc agctagttta tcataagcaa cttgagcgat tggattcgtt actgcactga
    23281 ttgataaagc gatcaagact tcattgatgc ttagtgcatc caaagaggaa tgaagtgtct
    23341 ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga caataaagga atgtcatctg
    23401 cgatatgggc taaatgtttt actgcattta agataactgc cgctgacgca tccatcaatg
    23461 aacttgtacg cccggtaatg atgacttcat ctggtaattc gattgcaatc acagcagaag
    23521 gatcttttgt aggactttgc gcttgaatct tatctgcata gtcatgggca gggactacca
    23581 cgtttcggtc ttcaggacgt aaaccgactt cttccataat cagtttgatt cgattaactg
    23641 cttcttcgtc gatcaatcct tttttgaagt cgcattctgt ttggaaataa cgacggatga
    23701 tttcttgttt agaggcttct tggacaacgg tatcatccac gatcccaaat cctacacgat
    23761 tcacacccat gtcagtagga gattgataaa cagattcttt accagtaatt ttttcaataa
    23821 tacgtttgat tacaggaaat gtctcaacat cccgattata gttcacagct actttattgt
    23881 aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt tagatcgact gtggctgctt
    23941 cataagcaat gttcagcgga tgtttcaacg gaacattcca tactgggaaa gtttcgaact
    24001 tggagtaacc agctgcatgt cctttttggc tttcatgata cagttggttc aaggaagtag
    24061 cgagctttcc acttcctggg ccaggagctg tcactacaac gattggttta gttgtttcaa
    24121 tatactcatt ttttccaaaa ccatcttctg agacgatttt ttctacgttg actggatagt
    24181 cttcgatttc tgagtgttta tagaccttga tgtttcgtct ttctagttta ttgataaaga
    24241 ctttcgtggc aggctgtcca ctatagcgag tgataacgac actgttgatt aataagccat
    24301 aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc ataagtaatc ccataatcac
    24361 cacggatctt attacgttca atatctccag catagacgca aatcagtatt tcagcttgat
    24421 ctttcaattt ttgcagcaat ttgattttcg catcttcttc aaatcctgga agtacacgtt
    24481 tcgcatgctt gtcgtcaact agcttcccac caaattctaa atataatttg tcatagtggt
    24541 tcactcgttc caaaatatac gctgactgct cttcaatata tttttctgaa tcaaaaccaa
    24601 tttttttcac taggttccct ccttaagtac taaagcattt tttcattttt ttgaggattc
    24661 gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa atggtttaaa ccaacttgtc
    24721 tagatagtta ttcattggta tgatagtttt tgataaggaa ggtgctacat gaaaaaaaga
    24781 cagaaaaaga aaaacgcata taaacactat atccgttcga tttttaccgg ttatgaaaga
    24841 atgcttgaag atcccgaatt ggaacaattg acgtttactt atctaaacga agaaacgcag
    24901 ctaactcgtg atgagcatca gcgcattcat ttcacaacaa gagatctgcc tagtaaataa
    24961 aaaataagat aaaggtcatc ggattcgatt ctgtcctatc agatggaaag actagttgat
    25021 cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt atggagcatt gcccgctctt
    25081 ttttataaca attttttaca gaaaatggta ctattgaagt attaataagt gaaaaacaga
    25141 tgagtaagag gaggcaagta gtatgaaaga gccagataca aatgcaaatt atgagccaga
    25201 cgaactagag gagacgaatg cggatcagga tacaggacaa gaatcgtctt ctttttacga
    25261 tatcaaagaa aaagcaagca tgaatctttc ccaattatta gaagacttac aggcttttga
    25321 aaaagctgtc cgcgaagaag atatccctga gatctaccgt ttatacaatg gccgattgaa
    25381 tgaagaatta aaaagaagct caaatgaaaa tcacgagatc gatcaattgc tgatgcgtaa
    25441 gatccatgat cgttttttac aggaatttcc ttttatggaa caagtagaaa acatctcgcc
    25501 aacgatgagc tattacaaaa tcggcactta ttaccatgat cggcccacgg tcggtattga
    25561 tgcaagtctg ccagaaattt ttgttttgcc taagatcgat gaagagtggg agaaatattc
    25621 ccaaccggag tcaacggact atgacaaaaa aatcaatgag ctagacgcaa aggtgatcac
    25681 tgctcagaca gaaattgaac gtttagatga acaaataaaa gaaatcaatc gacaaatgac
    25741 tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa atcgaggaag aaattcaaga
    25801 attagagaag aagaagcaag tactatctaa tgaaaaatta ggctggcttc cttatattga
    25861 gacgcctgaa acaatccagc agcaaaaaga agcattgaag caagaagcaa gagcagacca
    25921 gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc agacgctatt tcggtagcaa
    25981 agagggattc ggtcaagcaa tccatgaatt tcttgatgaa ttatcttggc aatgagaatc
    26041 ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga agcagaatgg
    26101 acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt ggaagaacaa
    26161 acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac aaaaatacac
    26221 gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca tgcttacgaa
    26281 ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga tatcgtcagt
    26341 ttgattgaaa cagcggaagc agaattagcg gatttacgta agagaaaaga tttgatcgaa
    26401 tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga agaaccaatc
    26461 tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact gaaaccgttg
    26521 aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac aagacatttt
    26581 tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt attaccgacc
    26641 atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc agagacaatg
    26701 gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc agtggatcga
    26761 gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt attagcccat
    26821 gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa gccacttcgt
    26881 ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat ttttatcgtg
    26941 gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta tcaagtacga
    27001 ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac agtcttgtcg
    27061 atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta cgaattcttg
    27121 gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat gtcattcaga
    27181 taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa gtcttttctg
    27241 tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt aggaatgtcc
    27301 acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg taggatcgct
    27361 tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat agtactttac
    27421 ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag cagtttttat
    27481 tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt tctgagtttg
    27541 ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa gggagtctgt
    27601 ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac gctgttatat
    27661 cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt gatgcacagt
    27721 ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat
    27781 tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg tcagtatact
    27841 cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa aggaggaagt
    27901 aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa
    27961 agaagtgttc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa attttgaagt
    28021 caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg agaaaaatca
    28081 actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca aagagatcaa
    28141 attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt cataaaaaac
    28201 tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga aggaaaataa
    28261 catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag gggctaatat
    28321 tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa
    28381 cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat tcggtgataa
    28441 acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag caaaatactt
    28501 tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg gggtgatgga
    28561 agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca ttgttatagc
    28621 aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat
    28681 caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag
    28741 tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg tcttggcttt
    28801 agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga tcagcggatt
    28861 gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat tttatagtct
    28921 gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag cttttgaacg
    28981 tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa cggagatttt
    29041 agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact cggtcatcaa
    29101 tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata tatacattga
    29161 aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc
    29221 aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat
    29281 gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa
    29341 gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat
    29401 accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc
    29461 aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct
    29521 tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta
    29581 tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc
    29641 tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac
    29701 tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg
    29761 ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct
    29821 tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc
    29881 actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt
    29941 ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct
    30001 ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa
    30061 tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc
    30121 tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt
    30181 attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg
    30241 cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg
    30301 cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt
    30361 cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa
    30421 ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga
    30481 actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag
    30541 tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct
    30601 atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt
    30661 aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta
    30721 aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg
    30781 gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc
    30841 ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag
    30901 gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg
    30961 gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga
    31021 ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta
    31081 tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg
    31141 gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt
    31201 acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt
    31261 ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact
    31321 tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg
    31381 cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta
    31441 tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga
    31501 tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga
    31561 cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca
    31621 cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg
    31681 cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca
    31741 cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt
    31801 ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc
    31861 gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg
    31921 gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg
    31981 tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga
    32041 tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg
    32101 ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt
    32161 attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa
    32221 atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact
    32281 aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc
    32341 tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa
    32401 aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg
    32461 catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga
    32521 ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac
    32581 ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg
    32641 gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt
    32701 cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa
    32761 cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg
    32821 ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa
    32881 tccgagataa tacctacgct tatctgattg tagatgattt gaacaattta gtacgaggag
    32941 gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga
    33001 cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg
    33061 ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt
    33121 acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa
    33181 aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt acacatttag
    33241 gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata
    33301 ccaaaaatat tccaacatca tcttggactt tctacatgtt actaatgagc gtgtaaaaga
    33361 acgagatggt gtttttattt ctatcaatga agctcttttt ctttgcaatc ctcacttcaa
    33421 tcctctataa tgtcgcatat agtttagtaa atgagtaaac tactaattta cttatttact
    33481 aaaaataaaa ggatgaggag ttaaaataat ggaaatacca acaaatttaa aacataaacc
    33541 agtaatcgtt gtagaagact atgataaagt tgatggaaga aatgcattaa atacggatgc
    33601 aaaagggctt tcactgggcc ttgctcagtg gaatgatcgt ggaaaagtcg atatctcagg
    33661 caaggtatgg cgacatactg gagaaaaatg gtcacgacag tcggaagaat taccaatcac
    33721 acgtatttta gatttagcga tcttagctgc acaaggaagt ttatacttcc aagatgctta
    33781 tcgttatgaa aaattttatg atccagaaaa tccagtcgta gacatcattg gattacaagg
    33841 gaaccgaatg acggtagaag tagataccaa aaatccaaag atcgatgaag acatcatttt
    33901 gtattacgat acactacaaa aagacggaga actgattggg gaacgattcc gtattttaaa
    33961 acgtctttta gatgagctag gatattaata gacagagatg atcaaaaggg gaagagaagc
    34021 atggagaatt tctcactaac agaaaaccga accttttcac ggctagaaaa gcaaatgatc
    34081 tggaagaaac cgaaaagcca tcggacaagt gcagaagaat ggcggatcgc ctccgaaatc
    34141 aaagggaatt ggcaagatcc tgaaatgaaa atcagtaacg tacttttgga aggagatgcc
    34201 gggtcgggga agacacaatt agccaaagca ctatctgctg atctgcaact tccctataca
    34261 aaagtgacct gttttgcaga tatggacaaa tcagatgttt tcggagcctt attacctgta
    34321 gtagattcta ataaggaaca agaccaagaa ttactagaag ctatttacca aacagataca
    34381 cttgaagctg tactgacact gatccagaac cattacggca ttgatccttt ttctgctaaa
    34441 gaaaaattag cagagctagt tcaaaggatc gaagctgatt atccagatgc aatccattac
    34501 agctattacc cttctgaaat cgttcgagcg attgaaaaag gatatttatt agagattcaa
    34561 gagccgactg tcatccgaga tgcctctgtt cttgtggcac ttaactcagc gctcgaacca
    34621 aatgggatgt tgaatttacc gacaggaatc attcgtcgcc atccagattg cgtcattgtg
    34681 atcactacaa atcgaaatta ccaaggaaat cgcccattaa atgaatcttt aagagatcgg
    34741 atgcaacatg cggaaaaaat ggatcttcct gccttagaag tcatggcaga acgtgcgatg
    34801 gcgaaaacta tgatccaaaa acaagaactt ctcttaaaga tggcagagat tatccgtttg
    34861 ttggatgaaa ctgcaaaagc taacgccatc aaaggtgtag caggaatgcg ttcttatttt
    34921 tattgggcaa atacctataa gcaaggacaa gatttactcc agtcgattta tccaaaagtt
    34981 ttgtataaat tatccactga ttcagatgaa ttggcaattc ttgaacaagc tttgtctgac
    35041 agtggattgc tactggaact tcaggacata ttgagaaaag aaaaatgggg tcatcctcaa
    35101 tctgagcata caaaaggaag aaccattagc gccgaagaag caaatgaacg aaacatcact
    35161 gaaaatgctg aagaaattct gcgcttagat gaaaagaaat cgttgtctgc tgaagaacca
    35221 aatgagggag aagagaaaca agaagaaaca atagaaaaaa acgagattcc tcctcaaatg
    35281 caaaaagaac gcagtagcga tatcgaagga aaaaatcaaa aagaacaaca aacaggagat
    35341 gattctcaag aaatgtctgc ttctgacatg gaagcacatg ataaggaaat caagaagcaa
    35401 ctgaataaag aagcgcgaca aatcatgaag gaaacgattc atgaaaaaga aggtctcatc
    35461 gttcaccgac cagctttcca aggaaaaacg ccggaagcac gtgagctaca catgcaagta
    35521 atgccaatag tagaatcact tagccggcaa attttagagt tactggataa tgaacaaaca
    35581 gagacgtacc aaaaaggaaa atacgaaggt cagcggttca atgcaagcaa agtagcatat
    35641 ggtgatttga gaaattttga caaaaagaac ccgccacatg agcaaccttc tttagcggta
    35701 gctgtacgga tcgatgaatc tgggtcaatg atacgggatg atcggatcga agcggcaaaa
    35761 aaagcagcga ttgccattgc cgaattttct aagaaagtcg atatcccatt gttgatttac
    35821 ggagatacag cagaccgttc gtcaagagaa aagacttctt tgttttctta taaagaattt
    35881 gaagacggat ttcaatggtt aaatgaaaaa ttagtcacta tgaaaccaag gcaaaacaat
    35941 cgtgatggtg ctgcacttcg tttagcagca gaaaaattaa acagacaatc cgcaacaacc
    36001 aaactgttgc ttaatattag tgacggtcag ccaaaagcgt tgccggatta tacaggcaaa
    36061 aaagcaaaag aagatatcca gtctgttttg aaagaatatg aacgtcaagg aatcctattt
    36121 gtctccgcag ctattggcca agacaaagaa gaaatcaagt cgatctacgg tgaaagtcgt
    36181 tttgtagata ttactgactt gaatgaattt cccaagcaaa tgattcaact gattgcccgc
    36241 tatctatgat aaaataaagc tcaacgaacg cacttagcag attcgttgag cttttcgttt
    36301 ctagttaccg gtaacaagga tacttctaaa atagaatcgg ttataatcaa gagggaccgt
    36361 taaaggagtg aaacgatatg atacaagaac aaaaatggtg gaaagaagca gtaggctatc
    36421 aaatctatcc tgctagtttc aaagatagca atggcgatgg tcaaggagac atcaatggga
    36481 tccgtgaaaa acttaattat ttgaaagatc ttggtatcgg ctttatctgg atcaatccga
    36541 tctatcaatc tccatttgtc gataatggtt atgacgtaag tgactatcaa gatatagaag
    36601 agagattcgg aacgatggaa gattttgatt tattgctaaa agaagcacat gaattaggga
    36661 tcaaagtgat tatggatctt gtcatcaacc atagttcgga tcagcatcaa tggtttgaag
    36721 aatcgaaaaa atctaaagaa agcccgtatc gcgattatta catttgggtc gacggggtag
    36781 atggcaaaga accgaataat tggacatcga ttttcggcgg ttctgcttgg gagtattctc
    36841 atgaaactgg acagtattat ctgcatgttt ttgcaaaaga acaaccagac ttaaattggg
    36901 aaagcgagaa attgaaagaa gaactattca atatgatccg atggtggttg gataaaggga
    36961 tagatggatt tcgtttagat gcgatttctc atgtaaaaaa agatgaatat tcagtaaaag
    37021 ccacagagaa ccctttctca ccctttcaaa atgtctcagg aatcgaagaa catttgaccg
    37081 atttgaaaca tgtttttgaa gagtacgata tcatgactgt cggagaagca agtggtgtca
    37141 ctgcagaaga aggaccacaa tgggtaggga aagacggtta tttcgatatg atattcgaat
    37201 ttgatcatat ccatatctgg cagcaagaaa aagaagggca attagatgtc cttaaattga
    37261 aacttgcatt aagcgcttgg caaacttcat tggatggtat cggatggaac gctttatata
    37321 tggaaaacca tgatgttcct cgtgccgttt cagtgtttgg agatactcgt cccgattttt
    37381 gggctatgtc tgcaaaagca atcgctatga tgtatttctt tttgcaaggt acgccattta
    37441 tttatcaagg acaggaaatc ggaatgacga atatgccctt tgaatcgatc gatcaagttg
    37501 atgcagtaga ttcgaaaaga ctttataaaa ggctgcttgc ggaaggtaag acaagagaag
    37561 aagcattgga tatcattcgt gaaacaacga gagacaacag caggacaccc atgcaatgga
    37621 cttcggaaca atatgctggt ttttctacac atgaaccttg gctgatcact aatccaaata
    37681 ccaaaacgat caatgtagaa caacaagaat atgaaccaga gtcggtcctg caattctata
    37741 agaacatgat ccgcattaga caaaccaata aaggtctgat atacggttct tataaagaat
    37801 atcttcatga acatccacaa ctttatgtct atgaaagata tttagaagat gaacaatatc
    37861 ttattatggt aaacttaaca gaatcacttg ctgactatga acttccaaaa gaagcagatc
    37921 agtcctggac attactcttg tcaaattcct ctagcggaga atttgaagct aaaggcatac
    37981 tagcacctta tgaagctcgg ttatataaaa caaacaagta aaacttcctc gttaaagttc
    38041 attagttgga ctttgcgagg aagtttttca gattgctctg tcaattactc atttcaataa
    38101 ttcgctctag gaccaccgat atatttagga cgagagcgaa gggctgtcac gtgagcttgt
    38161 ccaagggttt tcaaacgagg tctgacaggt acttggacat gtttgatatg cataccgatt
    38221 gatgtgtcac cgatatcgat accggcttgt ccaactacgt gttccacttc gacaggatca
    38281 ttgaactggt caaaagcagc aacagaacaa gcacctccag catgtagtgc aggaacaaca
    38341 gagacaattt cgaaattttt tgcttctgcc actgttcgtt ctactactaa tgctcggttc
    38401 aaatgttcac aaccttggac agctagcgca atttctttag gatccaacaa ctcttttaac
    38461 gtacggatga tccattgacc aacttccgca ctggaatttt ttccaatatg tcctccaacg
    38521 acttcgcttg tgctgcatcc taaaacaaaa agatcacctt ttttcaagtg tgcctcagcc
    38581 aatatgtcat taactatttc agtcaattgg tcttttaatt ctttttctga caacttcatt
    38641 ttctaaaacc ttctttttca attaatattg cttccgtgct tgaatacgtt gaagacttgc
    38701 agttatagga aaggcaagtc ccattccaat cagattttgg aagatgtttc ctggtataga
    38761 agccactcca gccggccaat catataaaat ccaaccggcg ataccatagc cgatgatcat
    38821 aactaagctt cctaataaaa gccctaatcc ccacatacga gtagaagatt tgctgaaaga
    38881 accagcaaca tatccttgta acccgtggat cactaatgaa aaaactgccc actgcggata
    38941 tcctgaaatc aaatcgatca atagaccact ggaagcacca acaactaatc cacccgttgg
    39001 accataaaga aaagcagcgg tatagatccc ggcctcgcat aatgtcacaa atccattcgt
    39061 agccggtaca gggatcaaaa ttgttaatga aagggcaact gtcaacgcaa tcagcatagc
    39121 atagatcgtt aattgcttag ttttcatttc tcattcctcc tttttgaata ccgccagacc
    39181 agacacttcc ggtatgatcg ttcatcagca cgccattaga aatacaatgg gcaacaaatt
    39241 ttttactttc tgctactgct ttggtaaaat ctgcccctaa cacaagattc gctgttattg
    39301 cagaagaaaa ggcacaaccg gccccgttta ctgttgtagt gtcgattttt ttatgagaaa
    39361 atacctcata atgatcaatt gaatagagaa gatccacagc ttcatctcca gccatccttg
    39421 tgccgccttt gatcacaatg ttttttgttc ctgaagcagt gagttcttta gcgagatgga
    39481 tcatttctgt taacgaagag ggaagagctt gaccagaaag taaagcagct tctgccaaat
    39541 taggcgtaat catcgtaaca agtggaaaaa gttcttcgat gagctgcgaa atatacaatt
    39601 gattgatcac agcttcagtt tctttgaatg ccaaaacagg gtctaaaatg attgggatat
    39661 tttgttttgt ttttatgaag tctcgaacaa ccatgattgc ttcacttgaa ttcaataacc
    39721 caattttgat accatctaat gaagtcattt tatcaatcga aactaactgt cgtttaagca
    39781 attccaaaga tatagattcc agcataaatc gctcattaat atctagtgcc cctatagaag
    39841 tcaaggcgct tagtccgaat agaccaaaat tctcaaaagt ttttaaatct gtttggattc
    39901 ctccaccagc aaatggatca gaaccgccaa ttgtcaagat tcttttcata acattttcct
    39961 ccatgaataa gagtataacg aaattttcaa aaaagaaaaa cagccaattg gcatattttt
    40021 atctcatcca atttaaaaaa acaaaaagaa aagaaagacc aaaatggatc tttctttcct
    40081 taaatagtta ggacttttgt gattttaatt ctttctcgat ttgaagccgt tgcactttca
    40141 ttgtagctgt tcgcgggatt gcatcaaaat cacggatgat agggacgttc aagcgaggaa
    40201 gttcatggac ttgctcccac catcgattcc aatccatttc tttatcagga gctaaagcaa
    40261 taatcggttg tggagaatga tttttatccc ttacaatcac tacttctgcc aaaaaatcta
    40321 acgaatctaa taaaaagtct tcaatagcta atgtactatt gatcgtttcg atcaaatcca
    40381 cttgccgatc tttcaaaaat aggtggccat gcttatccat aaaaccgtaa tctccgctat
    40441 cccaccaatc gccataaaca ttttcctgga accgtgcatc ttccttgtaa taagtcaaag
    40501 cccgtccttt agacagtaat tgtatatgac catctgtgtt ttcaggtagg acattcccaa
    40561 ctgaatctgt aatccgagcg cttgtcatgt cttccagtcc aacgcccata tctcttgcat
    40621 cacttgtttt caaactctct agtgtatgag ccttaagaat cattggtccg cattcacttt
    40681 ggccatatac ttgaagaaat accgcatcct gtgccatcga cgttcgcaaa aatgtcttca
    40741 ttgtctggtt gtttatagca tcaaaagtgg agtgatagta tcggataccc gaaaaagctt
    40801 ctggatgctc cttagcagta aaacgccact gaacaaagtt attcggatgg gtctctagtg
    40861 caattggccg gtgagcttcc aacatatgaa caacttttga actttgtgcg tttgctaaag
    40921 gcatcatagg aaatcccatg gacatcaaag aagagatacc aatattgaat ctggaatgca
    40981 ctggagagat gtgaaaagcg attaatttct tttccgaaat ttttgtgaaa actgtttttt
    41041 gccatttcgt ccgccatccc atagaatgag cagaatgaca aataagttta gggataccag
    41101 ttgtaccaga agtatgggtc atataagaaa tttcattttt gtctaaaaat gattgagaaa
    41161 cagacaaagc aggttgttcc attaaataaa gaatagg
//
LOCUS       DACEPW010000005        40215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEPW010000005 DACEPW010000000
VERSION     DACEPW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.005"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(194..991)
                     /locus_tag="IW819_000191"
     CDS             complement(194..991)
                     /locus_tag="IW819_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1243327.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(988..2172)
                     /locus_tag="IW819_000192"
     CDS             complement(988..2172)
                     /locus_tag="IW819_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1243328.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2197..3444)
                     /locus_tag="IW819_000193"
     CDS             complement(2197..3444)
                     /locus_tag="IW819_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1243329.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3659..5404
                     /gene="dnaX"
                     /locus_tag="IW819_000194"
     CDS             3659..5404
                     /gene="dnaX"
                     /locus_tag="IW819_000194"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1243330.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5424..5738
                     /locus_tag="IW819_000195"
     CDS             5424..5738
                     /locus_tag="IW819_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1243331.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5763..6359
                     /gene="recR"
                     /locus_tag="IW819_000196"
     CDS             5763..6359
                     /gene="recR"
                     /locus_tag="IW819_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1243332.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6423..7067
                     /locus_tag="IW819_000197"
     CDS             6423..7067
                     /locus_tag="IW819_000197"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1243333.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7082..7411
                     /locus_tag="IW819_000198"
     CDS             7082..7411
                     /locus_tag="IW819_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243334.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7425..8363
                     /gene="holB"
                     /locus_tag="IW819_000199"
     CDS             7425..8363
                     /gene="holB"
                     /locus_tag="IW819_000199"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1243335.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8399..9223
                     /locus_tag="IW819_000200"
     CDS             8399..9223
                     /locus_tag="IW819_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1243336.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9216..9563
                     /locus_tag="IW819_000201"
     CDS             9216..9563
                     /locus_tag="IW819_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1243337.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9632..10504
                     /gene="rsmI"
                     /locus_tag="IW819_000202"
     CDS             9632..10504
                     /gene="rsmI"
                     /locus_tag="IW819_000202"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1243338.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10613..11734
                     /gene="dinB"
                     /locus_tag="IW819_000203"
     CDS             10613..11734
                     /gene="dinB"
                     /locus_tag="IW819_000203"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1243339.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11788..12390)
                     /gene="nrdG"
                     /locus_tag="IW819_000204"
     CDS             complement(11788..12390)
                     /gene="nrdG"
                     /locus_tag="IW819_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1243340.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12705..14894)
                     /gene="nrdD"
                     /locus_tag="IW819_000205"
     CDS             complement(12705..14894)
                     /gene="nrdD"
                     /locus_tag="IW819_000205"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1243341.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15220..15987
                     /locus_tag="IW819_000206"
     CDS             15220..15987
                     /locus_tag="IW819_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1243342.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            15977..18019
                     /locus_tag="IW819_000207"
     CDS             15977..18019
                     /locus_tag="IW819_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1243343.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18300..18449
                     /locus_tag="IW819_000208"
     CDS             18300..18449
                     /locus_tag="IW819_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1243344.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18460..19974
                     /gene="dltA"
                     /locus_tag="IW819_000209"
     CDS             18460..19974
                     /gene="dltA"
                     /locus_tag="IW819_000209"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1243345.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19971..21176
                     /gene="dltB"
                     /locus_tag="IW819_000210"
     CDS             19971..21176
                     /gene="dltB"
                     /locus_tag="IW819_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1243346.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21221..21454
                     /gene="dltC"
                     /locus_tag="IW819_000211"
     CDS             21221..21454
                     /gene="dltC"
                     /locus_tag="IW819_000211"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1243347.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21457..22725
                     /gene="dltD"
                     /locus_tag="IW819_000212"
     CDS             21457..22725
                     /gene="dltD"
                     /locus_tag="IW819_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1243348.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22866..23948
                     /locus_tag="IW819_000213"
     CDS             22866..23948
                     /locus_tag="IW819_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1243349.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23948..24751
                     /locus_tag="IW819_000214"
     CDS             23948..24751
                     /locus_tag="IW819_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1243350.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24821..25561
                     /locus_tag="IW819_000215"
     CDS             24821..25561
                     /locus_tag="IW819_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1243351.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25572..26576
                     /locus_tag="IW819_000216"
     CDS             25572..26576
                     /locus_tag="IW819_000216"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1243352.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26621..28465)
                     /locus_tag="IW819_000217"
     CDS             complement(26621..28465)
                     /locus_tag="IW819_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243353.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28677..29195
                     /locus_tag="IW819_000218"
     CDS             28677..29195
                     /locus_tag="IW819_000218"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1243354.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29323..30006
                     /locus_tag="IW819_000219"
     CDS             29323..30006
                     /locus_tag="IW819_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1243355.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30315..30388
                     /locus_tag="IW819_000220"
     tRNA            30315..30388
                     /locus_tag="IW819_000220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30349..30351,aa:Arg,seq:tct)
     gene            30547..30906
                     /locus_tag="IW819_000221"
     CDS             30547..30906
                     /locus_tag="IW819_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1243356.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31029..32660
                     /locus_tag="IW819_000222"
     CDS             31029..32660
                     /locus_tag="IW819_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1243357.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32722..33363)
                     /locus_tag="IW819_000223"
     CDS             complement(32722..33363)
                     /locus_tag="IW819_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1243358.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33645..33953
                     /gene="rplU"
                     /locus_tag="IW819_000224"
     CDS             33645..33953
                     /gene="rplU"
                     /locus_tag="IW819_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1243359.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            33980..34324
                     /locus_tag="IW819_000225"
     CDS             33980..34324
                     /locus_tag="IW819_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1243360.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34337..34630
                     /gene="rpmA"
                     /locus_tag="IW819_000226"
     CDS             34337..34630
                     /gene="rpmA"
                     /locus_tag="IW819_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1243361.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(34723..35871)
                     /locus_tag="IW819_000227"
     CDS             complement(34723..35871)
                     /locus_tag="IW819_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1243362.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(35883..36194)
                     /locus_tag="IW819_000228"
     CDS             complement(35883..36194)
                     /locus_tag="IW819_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243363.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(36247..36642)
                     /locus_tag="IW819_000229"
     CDS             complement(36247..36642)
                     /locus_tag="IW819_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1243364.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(36678..37022)
                     /locus_tag="IW819_000230"
     CDS             complement(36678..37022)
                     /locus_tag="IW819_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1243365.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            37323..37544
                     /locus_tag="IW819_000231"
     CDS             37323..37544
                     /locus_tag="IW819_000231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243366.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            37671..38447
                     /locus_tag="IW819_000232"
     CDS             37671..38447
                     /locus_tag="IW819_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1243367.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            38444..38638
                     /locus_tag="IW819_000233"
     CDS             38444..38638
                     /locus_tag="IW819_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1243368.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            38640..38924
                     /locus_tag="IW819_000234"
     CDS             38640..38924
                     /locus_tag="IW819_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243369.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            38945..39133
                     /locus_tag="IW819_000235"
     CDS             38945..39133
                     /locus_tag="IW819_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243370.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            39459..39698
                     /locus_tag="IW819_000236"
     CDS             39459..39698
                     /locus_tag="IW819_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243371.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            39704..>40215
                     /locus_tag="IW819_000237"
     CDS             39704..>40215
                     /locus_tag="IW819_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243372.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKAL"
BASE COUNT        12769 a         6964 c         8119 g        12363 t
ORIGIN      
        1 caagaggttg tgaaaaagct gttctgcatc aagtaataag aagagccaag taaaaacagt
       61 ctcttatgtt tttaacttgg ctggacttat caccgtagat gtaagctttt gaacgccgtt
      121 tatttgcaac aaaaggggct gtgacacgat ttttgtcaca gccctttttt tgcgaacatt
      181 catttagcaa tttttagaaa cttctgccgc caccgccaaa agtcccgcca ccgctagaat
      241 gggtagtact tccgccaccg ccgcttccag gaggtgggct tttggggatt cttcttgtcg
      301 tgacaaaaga atttgtcaga cgatcggttt tttcagttaa tttgatactg gatttttctc
      361 ggaatggata tttgtagatg cctgatttca attgataacg cgaaacagtc acaaaataaa
      421 agacaagact aaggataaga gccaaaacaa cagcaatgat gatttctact gtcgtcaaaa
      481 ccttatagcg tgtaattttg ccagtttctt cgtctacacg ataatgtcct cctggaactc
      541 cgtcttccac atatgttgct gatttagaca ggtaagcttt tgcagcattg taataggcag
      601 catttgtcat ctgatcataa acatcatcta agatcgaatc gattctgcta tcagtcaaat
      661 aatcgatcat attaccagaa gtcgaaatgt agatttcacg ctgtcccata tctaatagca
      721 atactgagcc attttgatca tttccgactg cgtctcttaa gtaattatca gcaaagtctc
      781 ttggttcttc tgaattagat gtagtcgtta cgatcaagac tcgtcctttg attttttcat
      841 tcagcttttc tgcctgttgt tccaaagatt ggatttgatc tgttgtgaat aacccagcct
      901 catcatttac cgtagctgta tctgcatgag tagccaccgg ccagccaaca cttactagca
      961 aaagaaagaa taaaaaaaat tttttcatca tatcaagtaa cctccgatca gaaagaggat
     1021 cgctagccca gcgaaaatcc caagagcggt cagtcccaat tttttctggc tgatcggcag
     1081 gacaccgctg acctttcctg tttgtccatt cattgcgtag tagtagactt ttttacttga
     1141 gtcattgctt cgataagtaa caagccatac cggcagtaat acgtaattat ttacttcgtt
     1201 tgtgattgaa gcagaagttc gcacaccggt caatgttgta tatccatttg cggtatcttt
     1261 taacagcttc tctgaatagt cttttaattc agattgaatc tgtgatttga ttgcctcata
     1321 ttcgatatct cttttttctg cttggaaccc ggctaaatac tggcttttaa aatcaattgc
     1381 tttatctaac gggaaaggtt gcacgccttc gaccatcttt tgttgcgtat tttttgataa
     1441 ggcatttttt actagttctt taaaagaaag cttgcctgct cttgaaacag caaattgctt
     1501 cgtttctgtg tattcgatgt cacctatccg ccatatgcgg ataactgtac cattcgcctg
     1561 catggcacca tctacttcag catctgtagc ccaataaggg aaatacacac ctgtcaactt
     1621 atcgatttga tccttattga aaaaagcttt gggaacaaac cattttttct tggtccaagc
     1681 gagaaatctg ttgattgctt cttctttctc gacagcaaaa ggaagtactt tatttggcaa
     1741 gaattctccg cttaaacgtc ccgatagtac gacgggatta tggcagtagt aacaataagt
     1801 cgcagcagtt gtcgcatcag tcacgatctc tgcaccacag ttgggacaaa gaaaaagatc
     1861 tatcgctgcg gtattatctg cagattcgtc cgctgtatct gaaaaaccgc ctgcctcgtc
     1921 aaccgctttg cgctcggctt cattcatatc ggcaagctgt tcttgggctg taaaagtaaa
     1981 atcttcggta ggtgaaggtt cttctttgct ttcttcgcca gccatacgcg cttctttttg
     2041 cttttgctca tattggctga cttcttcttc tgtatagata ttcaggcagt attcgcaatg
     2101 aaatttttgg tctttgggat caaaagtcaa cggaccgccg caattaggac atttatgtgt
     2161 aagaacatcc atcagctgca cacctttcta aataaattaa ttaacgggga ttcctttaaa
     2221 cggtttacca cagtttggac agaatttagg gatgccgttg gaaagatcca cgatctcatt
     2281 gcattcactg catcgcattt gtagcttagg ctgaacgttt tctacaggtt tggcagctcc
     2341 gcagttggaa cagaattttc cggaattttc tgtcccgcaa actggacatg tccaagtatc
     2401 cgcatttcct tgtgcagttt gttgattttg tttttctgct tgttgtttga tttgttcttg
     2461 gttattttga gctgcttgag aaagataaga accatttgca ttcattccca tacctacacc
     2521 cataaagccg gtcattgctc ctgcttcatt ttttccggct gcttccatac cacgtgcaat
     2581 ggaaccttgc acgtatcctt cacgtacgct aggatcacct agcatcgccc cttcgttacg
     2641 catattgatc agtttcacag aatcatcggt gtaagaaatg ctggctacag ccacagaaac
     2701 aatctccatc ccacgtaatt cacgccaaga atcatccaat gcagtatcca tgtatttgct
     2761 taattccaaa ctcttagatg gtacataaga aatccgttga ccatctgcag acatctgatt
     2821 gattgctgat tgtagcgctg ttaggaattc agctaaatac tgctcattga tatcattgat
     2881 ttccacttgt gttttgtttt tagggattgc gtttgtataa aataaaatcg ggtcaacgac
     2941 atgaatagaa taagtgccat gtgcccgtaa gaataattct gcgttataga aattgtcaaa
     3001 gtagttcaac ggcgcgcttg taccgaattt gattcccttt atttcttgga gattgatata
     3061 aaaaacttgt tgtttctgcg gtgtgacgcc gccaaatttg aatcggtcaa aagtttcagc
     3121 aattgcttct ttcagtgttc cattgaacat ggaaggagct gcgtcatttt taatggtgta
     3181 ataaccttcc tcagcggtat aatcgatgat ttttccacca tcgaccaaaa gcatcatcat
     3241 gtttggatag acatggacga ctgttccgtc tgttaaaaca tcttctgttc ctttacgatt
     3301 ggagccacgt ttgtcatctt tgcggacttt gacacctttt gtcatgaccg ttgtatcact
     3361 catattgtct ggttcgatga cttctagcca ttgatctgcc agaccgccgc caaccatact
     3421 tgttgctgct ttgataagac ccataaaaaa ttcctcctct gattcagcta gtgccaaaca
     3481 attattgttt atttattata ccataggaag atgtgaaact tctcggacta gagacggaat
     3541 tgaaatggaa aattgcgtat tgtttaaata cctgaaaaag ccgagaagga tagtagtttt
     3601 tttacctaat atcacgtata ataagagata gtgaattttt taaaaaaggg ggatccttat
     3661 ggcatatcaa gcactttatc gagtctggcg ttcgcagcgt ttcgaagaca tcgttggaca
     3721 aaaagctgtg acacagacac taaaaaatgc tattgtttcc cacaaaacct cacatgccta
     3781 cttgtttaca ggacccagag gaaccggaaa gacgagtgca gcaaagattt tcgcaaaagc
     3841 aatcaactgt ccgaacagta aagacggtga gccatgcaac gaatgcgaaa tgtgtcgatc
     3901 aattactgct ggtacgcaag aagatgtgat cgaaatcgat gctgccagca ataacggagt
     3961 cgaagaaatc cgatttatcc gtgatcgggc aaattatgcg cctacaaagg cagcgtacaa
     4021 agtgtacatt attgatgagg tacatatgct ttcaacagga gctttcaatg ccttgttgaa
     4081 aacattggaa gagccaaaag aaagtgtcat attcatctta gcaacgaccg aacctcacaa
     4141 aataccagct acgatcattt cacggactca gcgatttgat tttaaacgga tcaatacggc
     4201 agatattgtc gaacatcttg aatttatctt gaaagaatcg actatcagct atgaaccagc
     4261 tgcattgaat gtgattgccc gttctgcaga aggcgggatg cgggatgcat tgagtattct
     4321 tgatcaggca atctcgttta gtgaaggaac aattacttta gatgatgcca tgcaagtgac
     4381 aggtagctta actgacgaaa tgatggacag ttacttgagt gcttgcgtga aaaaagaagt
     4441 accagaagca ttggaaacat tgaaccaaat tctcgcagcc ggcaaagaat cacgtcgctt
     4501 tctagaggat atgttacaat actgccgtga cttgctgatg tatcagcaag cacctaacct
     4561 agttgaggaa caatcgagta cgatcagaag tacatttaca gaattggcac aaactgtttc
     4621 agcggatcag ctgttttcaa tgataaagat tttgagcgat actcaaaatg aaattcgttt
     4681 taccaatagt gccgctattt atttggaagt cgctacagtc aaacttgcac agctaccgtc
     4741 tgcttctgtt ccttcagcag atgtagcatt tgcagatgat cagatagtaa aacagctaca
     4801 acaagagatt aaggaactaa gaaacgaaat caaggaacta cgcagtaata caggaattga
     4861 gaatacaaaa gtacagccgg tacagcaaac aaatcataaa aaacagacaa gtacctatcg
     4921 tgtaccgacg gaacgagtat ataaagttct gcgggaagcg acaagacagc atctgaacca
     4981 agtaaaagaa gtatgggaag acttgctgat gagtctatct gtcactcagc gagcaatgct
     5041 taaagcaagc gaaccagttg cagctggacc tacaggacta gtgattgcgt ttgattatga
     5101 gattgtttgt caaagagcag caaatgacga agaattgcag ctttcggtac acaacaactt
     5161 aagccgaatg atcaaagatt atgcgcctga tcctgtttat attacaagag aaagctggcc
     5221 agaacttcga agaaattatt tgacgcaggc acaagaagag ggaacagaaa actcttttgc
     5281 ttctgaagac tcagaaggag aaatcgagtt gcttcctcaa gaacaagaag taccagaaga
     5341 aacacttgta gtaaaagcag aggaactgtt tggctccgaa gctgttacag tggtagatga
     5401 ttaataaaaa ggagtagata attatgatgc gtggaatggg caatatgcaa ggcatgatga
     5461 aacaagtaca aaaaatgcaa aaagaaatgg cagaagcaca agagctgcta aataaaacag
     5521 agtttacagg taaagccacc aatgaattgg tcaaagcggt ttttacaggc gatcgtcgca
     5581 tgaaagatat ccaaatcaat gaagaagtcg ttgatcctga tgatatcgag atgcttcagg
     5641 atcttgtcgt aatggcagtc aatgatgcga tcgcacaaat tgatgtggaa actgagaaaa
     5701 aattagggaa atacgcaaaa ggtttgccag gattttagtt ttaaactgaa ggagtaatga
     5761 atatgcaata tccagaacca attgcgaaac tgatcgacag ctacatgaaa cttccaggaa
     5821 tcggtcagaa aacagcgaca agactcgcct tttatacgat cgatatgaaa gatgaagtag
     5881 taaatgaatt tgctaaatct ttgttaagtg taaaacgcga tttgcatttt tgtagtatct
     5941 gcggaaacat cacagaagaa gatccctgcg aaatatgtag aaatccttca cgtgatcgaa
     6001 gcgttatcct agtggtcgaa gagccgaaag atgtaatggc aatggaaaaa atgcgagaat
     6061 atcacggact ttatcatgtt ctccatggtg tgttatcacc gatggaaggg acaggtccag
     6121 aagacatcaa tatttcttct ctgttgcagc ggctgcatga tgcagatgta aaagaagtga
     6181 ttatcgcaac aaatgcaaca acagagggag aagcaacagc tatgtatctc tcacgtctga
     6241 tcaaacccgc aggtatcaaa gtgactcgtc tagcacatgg tctgtctgta ggctctgata
     6301 tcgagtatgc agatgaagtt actttattga aagctgtcga aggaagacgt gagttataaa
     6361 tttctaatta agtgtttttt cagatacaat ataaaagcag cacaagagat gggggcaaca
     6421 atgtgaacgg actatttatt acaatcgagg ggcctgatgg tgcaggaaaa accagcatct
     6481 tgaatgaact ttaccctctt ttgaaagaag tagcaaaaac agaaatcatt caaacaagag
     6541 aaccaggagg cattccgatt gctgaagaga tccgagcagt gatccttgat ccaaaaaatg
     6601 atcggatgga tgaacgtacg gaagcactgc tttatgcagc agcaagaaga caacatctag
     6661 tcgaaaaggt tctgcctgct ctggcaaaag ggaaaatcgt tttatgcgat agatttgtcg
     6721 acagttctct tgcttatcaa ggtgcaggcc gacgcatcgg cgttaccgag attgctcggt
     6781 tgaatgagtt tgctacagaa ggtactacgc cggattttac cctttatctt gacgtagatt
     6841 cagacaccgg attaagaaga atcaagaaaa acagacaaaa tcaaatcgac cgtttagatt
     6901 cggaaggttt ggaattccac cagcgtgtac gtcatgctta cttaaagctg gcagaagaga
     6961 atccggaaag aatccacaaa gttgatgcaa gaaaaagctt tgaagaagtg ctgcaaacaa
     7021 gttatcatac aattattgaa cagtatccgc aattttttga aaactaggaa ggtgaacgtt
     7081 tatgaaaatt attttagcga ttatccaaga caaagacagc aaccgtttat caaatgaatt
     7141 gattgatgca aatatccgtg ccacaaaact atcatcgaca ggcggcttcc ttaaggctgg
     7201 aaacagtacg tttatcgtgg gtattgaaga tgaccgtgta gaagaagcat tggaaatcat
     7261 caaaaaaacg tgtgaatcaa gaaagcaatt tgtatctaca cctgttacac tggatatttc
     7321 gatggatggg ggcgtccctt atccagtaga agtagaagta ggcggtgcca cagtcttcgt
     7381 cttacctgtt gaaggattcc atcaatttta ggaggagctg agctatgaat caagaaataa
     7441 acttggctca aatgcaaccg atcgtgtatc agcagcttca acggagtttt gagcatgggc
     7501 gtatcgctca tgcttatctt tttgaagggg agaaaggaac aggaaaacat gaagtaggta
     7561 tatggcttgc acagcacctg ttctgtaccc aaatgaaaga caatctgcct tgtggaaaat
     7621 gcaacaattg tcagcggatc gaaaagaaag aacatccaga tgttttggtt attgaaccgg
     7681 aaggacaaac catcaaagtc gatcagatca gacgtttgca gacggaattc agtcgcagcg
     7741 gttacgaatc aagaaaaaaa gtttttttga tcaaagaagc tgaaaaaatg aatagcagtg
     7801 cggcaaacag tttgttgaaa ttcttggaag agccccctgg agatttttta gcggtgttag
     7861 aaacagatgc agtaggaaga attctcccta ctattcaatc tcgttgtcaa gttctgcatt
     7921 ttcaagaact ttcgaaagaa gcactgatcc atcgattgca agaagagcag attccattgg
     7981 aaaaagcaaa acttttggct ttcttaacaa acagtttagg aaaagcagtt gaaatctctc
     8041 aagatgaatg gtttaatgat gctaaggact tgatttatca atggttcgtt tacttgcaaa
     8101 agcaagatac tcaagccttt atctatgtcc aaaaaaaatt ggtaaaatca tttaaagaaa
     8161 aaacacaaca gtttacagga ttatctgtct tgatgtttta ttatcaggaa gcacagcgtc
     8221 aagcagtagc aaacggcgaa tatggcaaag tgaaacagat aaaccagata attgaacgga
     8281 tattgatcgc agagcagaaa ctacgcagca atgtcagttt tcaggctgtt gcggaacagt
     8341 tcgttttgca tacgattttt tagcttgccg caaaggatga tgatcaagga ggatggaaat
     8401 ggtagaagta gtaggtgtcc gatatagaga tgcaggtcat atctattatt ttgcaccagg
     8461 agactctgaa tatgtttata gtgacaaggt attagtcgaa tctcagcaat ccttacatat
     8521 tgctactgtt gtccttccta aaaaagagat tgatcccaat gaccttcctg aagctatccg
     8581 tccgatcttg cataaagcgt cacaagctga attagaaaaa gacaaaaaaa acaaaaaaga
     8641 tgcttatgat gcattgaaaa tcgcaaaaga aaaagtgcag ttgcgtgagt tggaaatgaa
     8701 aatggtcaaa gccgaataca catttgatcg aaataaattg attttttctt tcacagcgga
     8761 cgggcgaatt gactttagag aattggtaaa agatctggcc gctgtatttc gtactcgtat
     8821 tgagcttcga caaattggtg tccgcgatga agcaaagata ataggcggaa ttggtccatg
     8881 tgggcgtcca ttatgctgtt cttctttttt aggcgatttc gtgcctgtat caatcaaaat
     8941 ggcaaaagat caaagtttat ctttgaatcc aacaaaaatt tccggtttat gcggacgctt
     9001 gatgtgctgt ctgcaatatg agagcgaaga atacgagaca gcgaaaaaag aactccctga
     9061 ctttggaaaa gtaattgcta cacctgatgg taaagggaaa gtcgtagggt tgaatctgct
     9121 tagccgtatc gttagtattc ggctgactgg acgggaaaat cctgtcgaat atgaatggga
     9181 tgaattgaaa gaatttatcc aagtaggtga gatgaatgga taagagatca ttatacgacg
     9241 gattgaatca attagaatca gagttgagaa atacattaac agaacttgtt gaaatgaagc
     9301 atgctttgca tgagatcgta gaaaaaaata caactttgga gatggaaaat caacgtctgc
     9361 gggaacacct gcgggaattg aaccaagttg cacaagctcc agctgaaaat gtgaagcagg
     9421 aactttctaa gtctcgtatg aatcttgaaa aaatttatga agaaggattc catgtctgct
     9481 acgatttata tggatcaaga agggacaatg acgagccttg tgcgttttgt ctagaagtga
     9541 tctatcgcga aagcggtcga tgaccaataa caagaggaga ctggagcgga gaaatactgc
     9601 tccagtcttt ttattaagga gattgtgaca gatgtataat caaaaaagtt tcaagggaaa
     9661 tccggtatat ggggatttat atcttgtacc gacgccaatt ggaaatttag aagatatgag
     9721 ctacagaagt gttcgtatgc tgcaagaatc cgatcttatc gcaagtgagg atacgcgtaa
     9781 tacacaaaag ttattgaatc attttgaaat ccatacaccc caaaaaagtt tacatgaaca
     9841 taattataaa gaacgtgtcc ctcaattgat tgctttgtta aaagaaggaa aaacgattgc
     9901 tcaagtcagt gacgcaggaa tgccttccat cagcgatcct ggacatgagc tagttttagc
     9961 ctgcataaaa gaagggattt ctgtgatctc tatcccagga cctacagctg ggatgacggc
    10021 tttgattgct tcagggctag tgcctcaacc atttttattt tatggcttcc ttccacgtaa
    10081 gaaaaaagaa cagaaagaaa cattgaaaaa gctaaaagaa cagacagtta cgctgatttt
    10141 ttatgagtca ccttatcgga tcactgctac attgacgaat ttcttagaaa tttttggaaa
    10201 tcgccaagtc gttttgtgtc gggaattgac aaaggtccat gaagaatacc ttagaggaag
    10261 tacggaagag ctactcgctt atatcgaaga acatccagta aagggtgaat gttgtctgct
    10321 tgtagaaggg aatttgttcg aaatgccagt gacggaagtt gatgaagagt tgggaacgtt
    10381 aaaggaacaa gtagaagcaa agatcgcttc aggcgaaaaa ccaaatgctg ctatcaaagc
    10441 agtagcgctg aaaaatgggc tgaaaaaaca agaagtctac cgtcagtacc atgaattaga
    10501 ctgagatagt ataagttgtc tataatgaaa gactaatgag gaaatcagct actaactttt
    10561 caaatctctt gaacgcgaac gaatgttctt gtataatgaa taagaggtga ggatgatgga
    10621 gctgcaattt cctttacata atgatacatc aagaaaaatc attcatattg atatggatgc
    10681 ttttttcgct tcggttgaag aacgagatca tcctgagttt cgcggacatc cattagtaat
    10741 tgccagacac cccagtgaca ctggtgggcg tggagtagtg actacagcaa attatgaagc
    10801 aagaaaattc ggaattcatt cagcaatgag tgcacaaaaa gcgtatgagc tttgtccgca
    10861 agccatcttt aagcctggcg accatcataa atacagcgat atttccaaac aagtacggga
    10921 agtattctat cgttatacag atattgtgga accagttagt atcgatgaag cgtatcttga
    10981 tgtgaccgaa aataaaatca attgcaagtc agccatcaaa atcgcccgaa tgatccaagc
    11041 agatatttgg gagagtgtcc agctaacatg ttctgcggga gtaagttaca ataagttttt
    11101 ggccaaatta gcatcagact atcaaaaacc tagaggaatc acggtagttg cccctcaaga
    11161 cgctgtgcca tttttaaaag ctttgccaat tgaaaaattt catggggtag ggaagaaaac
    11221 tgtacctaga atgcatgagc tagggattta tacaggaaaa gacctatatg aatgcacaga
    11281 aatgatgttg attcgaaatt ttggaaaaat gggctattct ctttatcgta aagtcagagg
    11341 aatccatgat tctccagtaa acgttacaag agagcgaaaa tctgtgggaa aagagcatac
    11401 ttatgggcag ccgttgcaaa cggaagaagc agtgctgact cagttgcgcc aactagctga
    11461 aaaagtagaa gaagcactga gaagagtcca aaaacatggg aaaacagtag ttttgaaggt
    11521 caggtacacg gactattcta ctgtgaccaa aagagtgaca ttgccagaat atatctataa
    11581 aaaagaagca ctcttttatc aagcaagcct tatatgggag gaaatcttgg gagtagaaaa
    11641 aggaatccga ttgttaggga ttactttgac gaatctggac ccgatgactt atgaaaacat
    11701 cgtccttcca ttatgggaaa atcaagaaat ataaaaacag ctttgagagt tgaaaccgga
    11761 aatccgtttt ctctcccaaa gctgttttta tatcaagtct tgctttttga tttgttcata
    11821 ggtttctgtt gcatccgtac atttttccca aatcacaact tgattggatt ctaaagattt
    11881 ttggacgtca atgatccgct ggttgctact tccgcgaaat tgtagtttta ggtcacgttt
    11941 gcttaattca aagcgtccgt caacaaggat atcgatttgc gaaaggagtt ctaatttatc
    12001 ttctgaatct tgtagcaatt catcaaaggt atagcctgtc catgaccaga tatcttttgt
    12061 cgtaccgaac tcttttcgga tacgtcgtac gacttgcaga caaacatctg tattcaaaaa
    12121 aggttcgcca ccaagtaaag ttaatccttg cacgtaatcg ttgcgcaagt cagcgatgat
    12181 ttgttcttct aattcaggtg tgaatggttt gccatatcgg aaattttgtg cagctttgtt
    12241 gaagcagcct tcgcaggcaa ataagcagcc actaacgtaa agactgttgc ggacaccttc
    12301 accatcgaca aaattaaacg gtttgtagtc agcaatatag ttttggctgt agtcttttgc
    12361 taaccattct tttggttttg gatttctcat aaaaaaactt ctttcattta aggttcaaag
    12421 atatagctat tgaagataaa atctactcga ccacataaat aagaacaaac tgacctaaat
    12481 ccattgttca agaaagcttg ttcttatttt gatagagggc gttcaaagat acaaagccat
    12541 cgaaagaggg tggtgaaaaa agacttgtca tagcctctta ttctgaataa acggtgttca
    12601 aaagcttgca tctgcagtaa taagcccaac taaacgaaaa atatgaggaa ctatttttgt
    12661 ttagctattc ttattacttg atgcaggaca gctttttcac aacctcattt catgtgtttc
    12721 acacgtgagg aaatttcttt atggcggcca tgtaccatcg gacgagcttg cggatttcct
    12781 aaatagccac aagtacgttt gactacatca catgtttttg gatcatgatt tccacattct
    12841 ggacattcga accctcgttc tgtcggtttg aaatcacctt cgaaaccaca ttgatagcaa
    12901 tgatcgattg gcgtattcgt accaagatac ccgattttgt catatgcaaa atcccataca
    12961 gcttctaaag ctttcggatt ttgttggagc atagggtatt cacagtagtg gatgaatcca
    13021 cctgaacaat attttggata atccttctca aattccaatt tttcaaatgg cgttgggttt
    13081 ttacgcacat catagtggaa actgtttgta taatattctt tatctgtgat attttcgatg
    13141 atgccgaatt tttctgtatc caaacggcag aaacggtcag ttagactttc acttggagtt
    13201 gaatagacac tgaaatgata accatattca tccccccatg catctgcatt agcttttaat
    13261 tctttgacga tatcaagtgt gaaagctttt gcttctgggt ttgtttccca ttcgccaccg
    13321 aagaaagctg aagcaacttc atacaatcca atgtagccta acgatacagt agctcgacgg
    13381 tttttgaata attcattgac agaatcggta cgtgaaagac gtttcccgaa agcaccatac
    13441 atatataaaa ttggcgcatt tgctggaatg gcttctttac aacgttctac acgatagaga
    13501 agcgcatctt tcatgatttc taagcggtca gacagtaact gccagaattt attctgatct
    13561 ccttttgatt cgatcgcgat tcttggcaaa ttcaatgtaa caacacctaa attcattcgt
    13621 ccgacattta cttcttgacc attttcgtct ttccaccctt gcaagaatga acgacagccc
    13681 ataggtactt tgaaacttcc agttaattca acgattttat cgtaattcag aatatcagga
    13741 tacattcttt tcgttgcaca ttccaatgct agttgtttga tgtcgtaatt aggatcttca
    13801 gggtttaagt tcacaccttt tttcaaagtg aagatcagtt tagggaagat agctgtccgc
    13861 ttttcactcc ctaatccatt gattcgaatc tgtaaaattg ctttttgaat ttctctttca
    13921 aaccagtttt ctcctagtcc aaatcctaat gaagtgaaag gagtttgtcc atttgaagta
    13981 aataaggtat tgatttcata ttccaaactt tgcatagcat cgaagatatc ttttttggtt
    14041 tttgcttttg catattcttt ttggcgttct ggcgaatcga tccattcttc cgcgtctttt
    14101 agatgttttt tatagttcaa ttctgcaaaa ggagcaagca attcatctgt tcggtcggca
    14161 gaacagccac catattgact tgacgctaca ttggcaataa tctgtgagat ttgtgctgtg
    14221 gcggtctgga tagattttgg cgattcgact tcggcattgc cgattttgaa cccattgttc
    14281 agcatgcctt tgaaatcaat caagcagcaa ttcgtcatcg gtgtataagg atgataatcc
    14341 aaatcatggt aatggatatc gcctttttga tgcgcatttg ctatatgtgg tggcaacatt
    14401 ttcagtccga ttgattttcc aacgatcccg gctgtcagat ctcgctgtgt attgaaaaca
    14461 ttactgtctt tatttgcgtt ttcgttcacg acagtttggt ccttgttgat tagcttttca
    14521 atcgaaaagt tgatatctgt tgctttactg cgagcaaaat ctcgtttggt acgataattg
    14581 atgtactctt ctgctaattc atattcgttt ttttctagta atatatgttc cacgatgttt
    14641 tgaatctcat agattttgac attttcagca aaccggcttt tgatttcttg gacaaccaag
    14701 tctgcaatca tttgaatttt ttcataagta agtggtgata atgagccgtg aattttcttt
    14761 tctgctttga tcagtgcttc attgatcttt tgttcatcga aatcaacatg gcggccatcg
    14821 cgtttgatga ctttcatctg ggatgtctct ggctgctgag taacgacagt tttttcttca
    14881 taatttactt tcattatctt caactcctgc taaattgttc ttgtctatca taaaacaaaa
    14941 tcggggaagg agcaactaaa tatcggctat atatagtatt caatgtgctg tataattgga
    15001 tttctatact atatgtagtg tatattttat tgtgaaaaaa ggtgaaaact tgtagctggt
    15061 ggatttgttt tttgcatatt taattttgct gaataaaata attgaataaa attttttatt
    15121 cagtccaagt aaaagtgaca aaaacataag agcaaattat tcgtagatat gctactatgc
    15181 aaataaatag aaatgaaaaa tgaaagagga ggagaagaat tggaaaagat gatcgaagtg
    15241 aaacatctaa gtaaaagcta cggcagcaaa ctttctcccg tcaaagcctt agataatatc
    15301 tcattcacag tagaagaagg cgagtttgtt gggattatgg gcccaagtgg tgcaggaaag
    15361 acaacgctga tgaatatctt gtccacgatc aatttgccta cgatgggcag tgtctcgatc
    15421 caaggaaaag aaatcacaaa aatgaaaaac agtgagctca gtgactttcg tcgaaaaaaa
    15481 ctaggattca tttttcaaga gttcaatttg atcgataccc taaatgcaaa agacaatatc
    15541 ctattgcccc tagcagttga acggatgact aaagaggaaa tggaaaagag agtaagacat
    15601 gtagctcaat tgttgaatat agaagaactg ctcaaacggt atcccgatga actttctgtc
    15661 ggacagagac aacgaattgc agctgctaga gcacttgttg ttcagccaca agtgattttt
    15721 gcagatgaac caactggctc tttggactcg aagtcggcta cagaattgtt gaactacttg
    15781 aagaccatga atcaaaaaga aaaagcaacg atactgttag tcacccatga tccatataca
    15841 gcaagctact gcgatagaat tttatttatt aaagatggtg tgattttttc tgaagttgtc
    15901 agaagaggga caagaagaga gttctttgag aaagtaatag acatgcaggc gacgattggc
    15961 ggaggaggaa aaatgaatgc tgtataaact agccgttaaa agtttttttc ggcagtcacg
    16021 aggctatctt gtttattttt tcagtttgac tttatccgta atgatctatt attccttcag
    16081 cgcaatgacc tacgatcaat ctttagtcag aagagcaagt caagatactt cgatagatgg
    16141 tggattgagt tttggtggat ttatcattac tgttgttttg ctttttttcg tattcagtgc
    16201 caaccgattt ttcttgaata gaaggcaaaa ggaaataggc atctatcagc tttttggtat
    16261 gaataaactg cagatatcag gtatatatgt gttagaaatc atgataattg gattgttcgc
    16321 ctgtatttct gggatattac tcggtattat tttttccaaa ctcttttcaa tgattttggt
    16381 tcgaatgatg gatatggatc ttactagtcc attttttatc tccattccat cagtagtaga
    16441 tactttattt gcttttttca tcatcctttt agcagtatct gtatattcaa tatggaaaat
    16501 atggcgctat ccaatgattc gaagttttgg agaaaaagaa caagcagata gttcaacaat
    16561 gcgcttcagg acaaggcacc gattactcgc agtcatcggg ttactacttt taacgagcgg
    16621 atattttgga gctagtcatt ttagggaaat cacttcatgg atgatcagtt atcattatct
    16681 tgatacatta gttttctttt ttcctttttt aatttttttc gtttgtgtgg tcggtactta
    16741 tttgtttttc tgttatacat ttcggttatt tttgatcata ttgagtaaat ggaagattta
    16801 ttactatggt atcaattttt tgataatggg taacacgcaa gttcatttgc tcaaaagttg
    16861 gcgtacaaat tctctgatta cagtcattct cggaatttct ttaatgacaa ttggcggcac
    16921 ggctagtatc tctactattt tatcctacca ggagaaagtt tcctccccga tggactatca
    16981 gtttgatgtt gagacagaga aaaaaatcaa gccgattcta gcagaggaag ggcagaagat
    17041 aactaaagaa atcaccttgc attataaagt cattgggagt atctatagtc aaaatctcga
    17101 agaacctatg tttcaaactg ctaatctgat cacggaaaca caatatcaag ccttcagaaa
    17161 agttaatcca gatttgccga acatttcttt gaaatcacct ggtcatactg ttttgcttga
    17221 tcaaatgcaa actctttttc ggaatatttc attatatgga tctagtattg gactgccaga
    17281 taatcaagaa ttgaagattc aacaaatgct tccaagtgta ttaggtgacg aaacaatggt
    17341 atatctaagt cctgtactga ttgtagatga taccgttttt gaaaaagcaa aaggtgtgga
    17401 ttatcaagta gtcaatgtag atgtatcagg tggaaataat gaaaaaatag atgaacgact
    17461 gagcaatgac ctgtcgataa agtggctcaa tgctgtttat tattcttatg atatcgtaaa
    17521 tggaagtcta caaggagaaa ttagcacaaa gaaacttccg gatgaccaaa tgaaggaagc
    17581 acaatttagc caagaaagct ctcgtttgaa tttctcttcc agctattcga aattgcgtgc
    17641 agttaatcgg cgaatcggta tcaatatttt tgtcacacta tttgtcggga tgatcgtgat
    17701 tattgctact ggtagtattt tgacagtccg gcaattagca gaagcagagg aagaaaaaga
    17761 aaattatcac ctattgacta agctggggat tccacaaaga aggatcagac ggatgatctt
    17821 cgagcaaaac gcacttacct tctttccacc catgatatta gggatcactc acgcagtatt
    17881 tgcaatcaat gtcttcactc agtatataaa aactgccgat tactggttgg cttatcttgt
    17941 gagtggctta ttgatcctta tctatctttt gctttatgtc attacgagtc agttatattg
    18001 tcggattatc gaggaatgat atacggaata agacaagata ataaatagaa ctaaattttg
    18061 aataccattt atttggaata agaggttgtg acacgatttt tgtcacaacc tctttatttt
    18121 ttatattttt taaaagtacg agaaattata aagaaaaaag cagtgatttg atgtgctatt
    18181 tttataaaag ctatgtgaca aaactgttag taaaaaaacg gtttctgtaa gctagacaca
    18241 tgtttctttt tgtcaaaatt agttacactt tgtgtgaggt tatatgagga gggaatccaa
    18301 tgaacaaatg gcgttctaat ccaaaattga attattgggg aaaattcatc attcgaacaa
    18361 ttttttatag tgcagtactg ctgttcctga tttatctgta tcactataaa aatattcaag
    18421 gaggaacttt tatctataac gaattttagg aggaagagta tggaaatcaa aacgattatt
    18481 gaagcaatcg atgattgggg aataaatgaa cctgatcgag ttgcttatca ggcggatgag
    18541 acgcatactt ttggtgaatt gaaacaagca tcagacgcac ttgcttatta tttagaaaaa
    18601 aaggtagaag gcgatggacc aattgtcgtt tttggcaatt tggagtttga aatgattgtc
    18661 tcatttctag gggttgtcaa atctggccat gcctatttgc cgattgaaga gcatacaccc
    18721 aaggagcgta tcctatcgat ctttcgggtg gctaaaccat ctatggtcat cagtatcggg
    18781 gactggatag aagatattcc ttcggttcct gtcataacaa aaaaagaatt tcaagaaatc
    18841 aagaagatac cagtaggatt ttatcccaga aatagcgtaa aaggtacaga aaatttttat
    18901 attattttta cttctggtac tactggagaa ccaaaaggcg tacaaatcag tcatgataat
    18961 ctagtcagct ttgttcaatg gacgatggaa gactttggga tcaagcctgg tatgcatttc
    19021 ttagcccaag cgccattttc ttttgattta tcggtatttt ctatttatcc tgcattagtt
    19081 tctggcggaa tgctgaagcc gttaaataaa gcagttgttc aagactttcg tcaattattc
    19141 gcgacattgc cagccttgaa gttgaatgtg tgggtttcta ctccatcatt tatggatatt
    19201 tgcttgatgg aacctacatt caatggagaa aatgtgccgg aattggacat gtttttattt
    19261 tgcggagaag aattgacgaa aaaaactgca gaaagtctac ttgatcgatt cccaaatgca
    19321 cggatatata atacttacgg tccaacagaa gcaactgtag ctatttcaag tattcagatt
    19381 gaccagcatg tactagatag ttatgaccga ctaccgattg gctacgtaaa aaaagacacg
    19441 aaagtttcta ttgtccaaga aggtaaacct gtttcaaaag gagaaacggg tgaaatcatt
    19501 attgcaggac caagcgtatc aaaaggttac ctgaacaatc ccgaaaagac agctgctgcg
    19561 ttcttttgtc aaaatgactt agcaagttac catacagggg atgcaggtag actagatgaa
    19621 tatgggcttt tattttatga aggacgtatg gatcagcagg tcaaattaca tggataccgg
    19681 attgaactcg gggatatcga acattatctg ctccaagaca accgaatcaa acaagctgtc
    19741 gtcgtaccaa aataccaagg aacaaaagta cagcagctgg ttgcttttgt cgtattagaa
    19801 aacaaaacga aacagccgga ttttcagttg actaaatcta tcaaagaaca attactagaa
    19861 gttgtaatgg attatatgat tcctcagaag ttccattatg tcgatctgct gcctcagaca
    19921 gttaatggaa aaatcgatcg aaaaaaatta acagctgagg tgaatcctga atgatgttac
    19981 ctcatatgat cccttatgca gagccgtttt atttttttct tttagccatt gcgcttgtac
    20041 cgatcgttct atctttattg atatgggaaa aaagattacc agtttatcaa tcagcagtga
    20101 cacttttctt tttatttgtc agctttggag gtaattattg gcaacaggga gcggcgctga
    20161 ttgggtatgt tttgtggcag acgatacttg tatggggcta ttttgtttat cggcaaagga
    20221 aaaatcagac aggatggttt tttgccgccg ttattttagc gataattcca ctgtttttaa
    20281 cgaaagtgac tccttttttt gaacatggaa aagcatcgtt acttggtttt ttaggaatct
    20341 cttatttaac ctttaaatct gtacaaatga tcatggaaat aagagatggc atgatcaaaa
    20401 catataagcc atttcgttat atccagtttc tgctgttctt tccaacaatc tcttctggac
    20461 caatcgatcg ctataggaga tttgaaaaag atcagctcca tccgcctgtt cgtgataaat
    20521 atgttgatct tttgggaaaa ggcgtccaaa atatttttgt cggtttttta tataaattca
    20581 ttatcggtta ttattttggt caagtactgc ttcctgtagt gggacgcatt gccttacagc
    20641 atggtggtat ttcatgggca ttagtaggtt atatgtacgt gtacagtatg tatttgtttt
    20701 ttgattttgc tggctatagc ttatttgcag tcggtacaag ctacatgatg ggatatcaga
    20761 caccaatcaa tttcaataaa ccttttttat catggaatat caaagaattc tggaatcgtt
    20821 ggcatatgac gctgtccttt tggttccgtg attatgtata tatgcgattg atgttcttca
    20881 tgatgaagaa aaaatttttt aaaagcaaga tcgtaatgtc aaatatcggt tattttgctt
    20941 tgtttcttct aatgggtata tggcatggat tgacgtggta ctacatcgta tatggactgt
    21001 atcatgcttg tctgatctgt ctgacagatg catggatccg atttaagaag aagcataaag
    21061 agcagttgcc ttctaacaaa tttacgcatg gcttagccgt atttttgaca tttcaagcag
    21121 tatgttttag cttcctgatt ttttcaggat ttttagataa actttttttt agctaacgaa
    21181 tagtgacata ttaatttata taacaaagga gaaaaggaaa atggaagaac aaattttatc
    21241 aatcttagaa gaaataacag gaacagatga agtaagaaaa aatcgtgata ttgatctatt
    21301 tgatgaaggg ttgcttgatt ctttagcatc tgttcaatta ttagtcgagc tagacggaga
    21361 gctggatatc caagtccctg tatctgaatt cgagcgagag gattggagca caccgaataa
    21421 aatcattcaa caagcaactg cattgaaggg ttgagtatga aaaagaaact ttttgctatt
    21481 ttcggcccgg ttgttttagc tgttggctta ctgtttgtgt tttttacctc ctcctatcaa
    21541 gtagatatct ctaatccaga aacgattaga cgtgcctctt cttccatgtc agtgaacgtg
    21601 ttgaaaggag atgcgataaa aaattatgca ttatctgaaa aacaatatat tccttttttt
    21661 ggctcaagtg aattaagcag aatcagtcca tttcatccgt ctgttctggc agaaaaatac
    21721 cagcgaaact atcgtccatt tttgctggga gcacccggga cacaatcgtt aagccaatat
    21781 atgatgatgc gttctgcagg agatgctatg aaaaacaaga aagtcgtctt tattatttct
    21841 cctcagtggt tcgttaaaaa tggtgtaaaa actgattact ttaatacgta ttattctgaa
    21901 cttcaaacat acgactggtt gttttcaatg aagaaagtaa cgcctgctga tcgctattta
    21961 gctagaagat tattaacttt ctcaaaagta aaagagaatg atacgttgac ggcgattctg
    22021 caaacgatca aaaaagggaa gctaccttta cctgaatctt tgaatcagct gcgcagccaa
    22081 tggaacatgc tgaagagaga ggatgaagta ttcagcaata ttgggttgaa agatcgacag
    22141 caaaaaatcg atcatgagtc aaaacgatta ccaaaacaat accaagaaac agaattatcc
    22201 attttagcaa atcaaattgg agaacgagaa acgacgaata atccattcgg gttgaaaaac
    22261 gatttttata cgcatcgtat ccgagcccac gagccagagc tcaaacagtc acaaaaaaat
    22321 tgggattatc gtttttcgcc agaattttct gattttcagc tggtcttgga tcaattagca
    22381 aaaaaccata acgaagtatt gttcatcatc ccaccggtta acgagaaatg gagcgattat
    22441 accggactat ctcaagaaat gttacaagga tttgccaaaa agatcaaatt tcaattaaat
    22501 agccaaggat tcaatcgaat agctgatttt gtcaatcaag cgggtaccaa ctattttatg
    22561 gaagacacga tccatctagg ctggaaaggc tggttggctg cagatcaaca gatcagaccg
    22621 tttttagaag aaaatcatat tactgctagt aagtatcatc ttgacgatgc tttttttagc
    22681 aaatcttggc aacatcaaat accagataaa ctacaattaa aataaagaaa acaggcgagt
    22741 gttgctcgct tgttttcttt atttttgaat attctgaata aaagataaaa ataccaagtt
    22801 tgcctgaaca aaaatcataa gcaatggtat aatcacaata tgaatataga aatgggggaa
    22861 aacatttgga tacaaaagaa gaacagctac ttattgattt aacttctgca aaaggagtac
    22921 ctggaaatga agaagaggta agagaagtat tcagagaata tgccaaacca ttcgctgatg
    22981 atatttttta tgacggttta ggcagtgtga ttgccaaaca tggtgctggc ggaggcccca
    23041 aggtctttat ttctggacat atggacgaag ttggttttat ggtaacaaaa ataaccgaaa
    23101 aaggattctt ggaattccaa actcttggag gttggtgggg gcaagtcatg cttgctcagc
    23161 aagttgaaat caaaacacgt gaagggaaaa tcgtccacgg tgtgattggt tccaaaccac
    23221 cacatgtttt aacacctcaa gtaagaaata aaccttatga aataaaggat atgttcattg
    23281 atattggcgc atcaagccaa gaagaagcca aagagtgggg catacgtcct ggagatatgg
    23341 tcactccata tattgaatac aaacgaatga atggcagtaa atatttactg gcaaaagcat
    23401 gggataatcg tatcgggaca gctgtttctc tgcgagtatt agagaatcta tctaaagaag
    23461 cacacccaaa tgttttattc gcaggaagcg atgtgcaaga agaagtaggc ttgcgaggtg
    23521 ctcgaacaag tacacacctt gtaaatcccg atattgcttt tgctttagat actggtacag
    23581 caggagacac tccaggaatg acaccaaaag aagcagattc gattttagga aaaggcccac
    23641 agattttgat ttttgatgca tctatgatcc cgcataaaaa attattgaat tttgttattg
    23701 atgtagcaga ggaaatggat attccattcc aatatacagt gatttctggc ggaggaactg
    23761 atgcaggaca aatgcatttg acaagagatg gtgttccgtc tgtagccatt accgttcctg
    23821 ttcgttatct gcattcccat acatcagtga ttcatgaaga tgattatttg aatacagtga
    23881 aactagtcac agaattagtg aaacggcttg atagtgaaac agttgaaaaa attcggagtt
    23941 atgaataatg aataatagaa tacctatttc tgaatatcgg aaaaaagcac gagtttcttt
    24001 ggaaaatcaa tggggaatca atacttggct tgttttctta tctataatat ttacgagcgt
    24061 catccagaac ttttttgacg gtttcagtcc ttttccggaa gaaagcatgc agcagcactt
    24121 attgagtttt ctagtcgata atttactttt attcgcattc aactatgcat tgttttacat
    24181 tgcgctagta gttgtacggg gtggcagacc aaaaagtaac cttttatttt cagttttcca
    24241 aagaaagtat tttggttcga tacttggcat caatttgatc aattcagtca tcaactggct
    24301 actcaacagt ttaatctttc tgcccatgtt tttaatcagc ggagtgagta cttatatgca
    24361 gctgcttttc agttcaaatg cggtacctac gggtgtcatg ccagaagatt ttcttgatat
    24421 cagctttgtt ttatcgatga tagggatgat ttttgtttca gcactcatca tgagtattgt
    24481 tggcggttta ttccaatttg ctgcatggac aaagttcgac tatcctgatt tgtcggttat
    24541 tcaatgtttg aaatatggct ggtttttatt gaaagataga attggtacgt atattttgtt
    24601 gcagctgtct tttatcggct ggtatttgct aggagcaatc gctttgttca tcggtctttt
    24661 gtgggtcatt gtttatgtga atgtgtcagt tgcagaattt tatgaacaag cacggattga
    24721 aaaaggaggt ccagacgcct attttactta gcatgtaagc agtttattca tcaaaaaaat
    24781 aacttttgtg gtaggataaa tctttgaggg ggaatgtacg atgaaattaa ctgttttagg
    24841 atgcttagga gcgtatcctt ataaaaatca agggacaaca ggttatttgc ttcaatctga
    24901 cgggttcaat ttgttgatcg atgcaggtag tgctacgctg atcaaattac aagaacacct
    24961 tgatccactt gatctagatg ctgtgatcct tagccattat catcatgatc atattgctga
    25021 ccttggtgta ttacagtatt actggcaact tcatcctaag agaaatccgg agacattgct
    25081 ttcaatttat ggacacacaa aagattccat tcactttgaa gaattgacgt tggaaaatgt
    25141 ctcaaaagga catccttatt ttgaagctga agaattaaaa attggtcctt ttctgattac
    25201 ttttatggaa acaatccatc cagtagtatg ttacgccatg cgtatcgtag aagaaacaac
    25261 ggggaaagta ttcgtattta caggtgattc aggttatttg gaaagtttcg ctgattttgc
    25321 gaaagatgct gatttgtttc tagcagatac ctatcttttt gaaggaaatg aacatcatca
    25381 tgcccatttc acttcaaaag aagctggaga acttgcgaaa tcagctaacg taaagaaact
    25441 tgtacttaca cacctacctc agtttggtga tttagaacaa ttgaaaaatg aagcgcaaaa
    25501 ggcagcaggc aaagggattg atatagaact tgctgcagtt gacaaagtgt ttaacattta
    25561 ggaggaagaa catgattttt gtaccaaatg aaaaccacga cccaagaatc aatttagcaa
    25621 ttgaacaata tttgctgcaa gaaatgcctt tagatgaacc tattttgctc ttttatatca
    25681 atgaaccttc aatcatcatc ggcagaaacc aaaatacgct tgaagaaatc aatcgagatt
    25741 atgtggaaga aaatggcatt catgttgtcc gtcgcttaag tggaggcgga gctgtctacc
    25801 atgattttgg aaacctgaac tttagtttta tcatgcctga tgatggggat tcattccgtg
    25861 actttgcgaa agtaacacag ccaatcatcc aagcacttca tgaattgggt gttgctgggg
    25921 cggaattgaa aggccgaaat gatctagtca ttgatggcat gaaattttct ggcaacgcga
    25981 tgtatgctac caacggtcgg atgtttgctc atggaacaat tatgttcgac tctgatatca
    26041 atgaagtagt caatgcatta aaagtaaaaa aagacaaaat cgaatcaaaa ggaatcaaat
    26101 cgatccgttc tcgtgtaacg aatatcaaac catttcttcc agaagaaaag caaaaaatga
    26161 caacagaaga atttcgtcaa gaaatcttgt tgaaaatttt cggcgtagag tcagtagaag
    26221 aagtaaaaac gtacgaattg actgatgaag attggaaaaa aatcaatcga atctctgagg
    26281 aatattatcg aaattgggat tggaattacg ggaagtctcc agatttcaat ttctctcgcc
    26341 aaaaacgttt ttctatcggt tcgattgaag tccatttgaa tgtctcagaa ggaatgatca
    26401 aagaagcaaa aatctttggc gatttcttcg gactaggaga tatctctgac gtggaaaaaa
    26461 aattggttgg acagaaatat gataaagcat ctctatcaaa aatagtcagc caaattgatg
    26521 tgaagaaata ttttggtgcg atagaaccag aagaattgct taccttactt tattgaaaat
    26581 aaaaaatagc ggaatccaca tatatggatt ccgctatttt ttatacagga ttcgggtaat
    26641 ttcgagagag ttgaacagtg atccgatcaa tgaaagtatc tttttcctgc gttagtgttc
    26701 ctgtgtaacg atccgctacg tctttcatca tcattaagtc aaaacgttcc tgatcatcta
    26761 attcttctgt gatgcctcga ctaaataggt ttgtatcgta atgtgcacta tgttcgcaac
    26821 aaatgacgaa atgttcctct tgttcgtgta tcgacaaata aatttttcga tacagtggat
    26881 tttcgatggc atgagtttct ctaaaggaat gctctaaagc gtggatcaaa agttgagcca
    26941 gatcgtcatc gttgatagga agttcttcgg gaagatctgc ttggaaggtt atctgaatat
    27001 tccgtttggc agctaacttc tggtaacgcg ctaagataag atttgtcacc tggtggttgc
    27061 aaaaagaaca cagcatttcg aaattttttg cttcttctaa tatcttttcc acatagtttt
    27121 tagcttttgt ttggtccgta tgcattaaat cttgcagatt ctccatctct tggacaaatt
    27181 ccaggcgaag tgtatttact tcttggatat gctgtcgaag ctgctcataa tgttgctcca
    27241 acaaatcatt tttgattttt aagaaatcta cttgtctccg atctttgtcg gtatttaaga
    27301 tgtgatcgat tagattgtag ctgataataa cgattagaat gataatatat aaaatcgtag
    27361 accaattgat cgttgtttga tagataccta atgccgtttg gatgtaaatc aagcaatgga
    27421 taatggctac caagacgatc caaggtgtac aagctacata aaaacggtta ttcttatatg
    27481 cttcgccaat gcatgcaagt gaagtgacta aagtgcttat aacactagca atattagcaa
    27541 tcggcattaa ttcaggaagt tctgcaggag taaaagttgc ataaagcaga acgccgataa
    27601 aaatcaaaag ttggaacgta caaaaaattc cgtaccattt tttatagtaa aacattcggc
    27661 tccaatagta gcagatgatg aaagctgacg ttaagataat aaataaatga gataaaaaag
    27721 aatgaacgaa tggttcaaaa agaataccac ttaaaatatc ttctgataca gcttcaaatc
    27781 ctaatgccaa agcaaagcaa cttaagagaa taagtgagta ttcaagacgt ttttgtttat
    27841 acatcaagta gatcgtatag aaaaaggtcc ctatagcgat aaaaacagcg attatcatcg
    27901 tcaatacttg cggcactgat tgtgaataaa taaaactaat caatggtcca gttgttccta
    27961 gataaacttt cggtggcagt ccagcatagt tctgataagg agatgagact gtaagggtca
    28021 atttttttcc gatataattt gctggtaaat ctatcacagc caatgagagt ccgggattgc
    28081 tttcttcctg ataatctttc cgttggtata aaatcttttc gcctagatat acagtaatcc
    28141 attgatggtt tcctttgatt agtaaatttg cttcgtccaa cggtttttca agtgtcgttt
    28201 ccataatgaa tgtttcgtct ttatccacgg taggaatcgt acgagtgtac ttgctgtcaa
    28261 acttaaaatc aggatcatct tttgtataga aaacccacga atcttttatt tctttcattt
    28321 gattgctgtc cgcaggagaa aaaataaaat aaaagaaata agcaattcct gttaaaaata
    28381 acaatgataa tataatgatt atgctccact ttatacgtaa ataaaaagat tcttttttat
    28441 tatatatgtt attaaaaaaa gacataaagg tattcctttc taaaacttgt aatgttaggg
    28501 taaacgtttt ttaaactaac gtcaattatt tataataaaa aatgagataa agatgaggaa
    28561 atagaattag aaacatataa ttattaaagg gaaacatttt tacagctata atttgaaaaa
    28621 aatttatcgt tattatatga ttaagaagga ttgaataaag aaagaagttg aaaacgatac
    28681 ctgattacaa aaaaataact tttataagtt ctataacgtt gattatattt ttaattctta
    28741 ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac tccttatatt attgaggaca
    28801 aagaaatgag tccatattat gcacaaggaa atctgatata tgtacgaccg aaaaatacaa
    28861 atgagatttt agtcggagat attattacat attatgaaaa ttcaggaaaa aaaatctcga
    28921 cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt ttatacaaaa gctgatacaa
    28981 agacttatat tgagcctgga gtagtaaaaa agagaaatat tatcggtagt cccatctttc
    29041 agattccata tattggacta gtcctttcaa aaacggcatt tgcatggatt caggggttat
    29101 tctttacgat tgctttttgc ctaacaggaa tcacagcatg gaataccttt gtagagttga
    29161 aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg ttaaaaacat aaatgtattc
    29221 cagagatcca acgactcttc tgaacatgaa acacgagaaa acactcatgc agcttgtaga
    29281 tttagtcagt tgttcgtata atgtgtgaag aggaggagta gtatgcgcaa tgaaatgatg
    29341 catcgtcctg tagtcaaaga agaactattg gattttatgc gtacccagca aaaaaaatta
    29401 ccgggtgagc taggcaaact ggaagaagaa gctcatgtag cagaagtacc agttattccc
    29461 catgaaacag ttgtattttt gaaattttta cttggacagc ttcagcctga acgaatcttg
    29521 gagatcggag cagctatcgg tttttcatca agtgtcatgg ccacgacaat cggagaaaac
    29581 gctcatgtta cgacaattga tcgtttcgat gtcatgatcg agaaagctaa aaaaaactat
    29641 gaacgtctag gactgacaga taaagtgact ttgcttgaag gtcaagcagc agatatcttg
    29701 ccggagttgt ctggtccgta tgactttatt tttatggata gtgcaaaatc aaaatatatt
    29761 gaatttttac ctgaatgctt acgtttgtta agaaaaggtg gcgttttgat ggtagatgac
    29821 atcttccaag gaggaacgat cttgctccca gatgaagaga ttccccgtgg caaacgagct
    29881 atccatcgta aattaaatga atttcttcgt gttgtgatgg atcatcccga tttgacatcg
    29941 acaatcttgc cattaggtga tggtgtgatc ttaatgacaa aggaatcaga aacaatcgag
    30001 ttgtgataca tgattatggc agcctgtaga taatcgattg aatcgagggc tcaattcgaa
    30061 cttgatgtaa aagtttctat atttgtaagt caattaaagg atacaggaaa tctaaataaa
    30121 caggagtagc atgaagtcaa tgtagctcct gttttcaatt gataacctta gaacagctga
    30181 aggagaatgt ctcgttttaa aagattgatt tagtagttgg gccaaaataa ttcattattt
    30241 gctaattaaa taactatttc gtattgacag aagcaattta attatctata attattgatg
    30301 ttccgctgat tcttgtcata gtagctcagc tggatagagc atccgccttc taagcggacg
    30361 gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt tagaaactga ggtttctaac
    30421 ggtttttctt tgttttttgt gccaatattg tttgttttca aaggcaaata aaataaaaaa
    30481 acatccttat cgagtacaat tataaggagc aaaaaaataa aactcatcag aaaagaggaa
    30541 gaagatatga cgatactatc tagaatttta gtgactctag ttgcaattga atttttttat
    30601 atcatgtata tcgaaacggt gagaactgat tctgacacga caagtagggt ttttaaaatg
    30661 agtaaagaag agttaagcag aaaatctgtt caaacattgt ttaagaacca aggagtgtac
    30721 aatggactat taggagtggg actactttat ggagcttatc tttcttccgc atctaaagaa
    30781 atcacaagta tgctattaat atctattttt tttgttgcat tatatggcag tttgactagc
    30841 gatcgttgga ttttactcaa acaaggtggg ctagctctgt tggcattgct ttctattttc
    30901 ttataaatat agaaaaaata agaatttatc taacaggaaa ctttgtgaaa aatattactt
    30961 tatctcatga aatatgatat tctaatgaat ggtattttag aaaatttcat gaaaaaggag
    31021 ttagataggt gttcaaaaat tatgtttcct cactaaaaaa agaattttca ggttatgatc
    31081 tttcaagatt tcagaaagat cttttggctg ggatcacagt agctgctgta gcattgcctt
    31141 tagccttggc ttttggggta tcaagcggag ctgatgcggc cgcaggtttg attacggcta
    31201 tcattgccgg tatcgttatt ggcggtttat caggcggttt ttatcaaata tcaggtccga
    31261 caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac aaagggaatg caaggtgtat
    31321 tgcttgctac ttttttagcg ggtctatttt tacttgcagc aggacttttc cgattaggaa
    31381 cactgacttc ttttatccca gcacccgtaa ttacggggtt tacttcaggt attgctatca
    31441 ttattgcatt aggtcagatc gataatttat ttggtgttca ctcggaaggt acaaacgtta
    31501 ttgaaaaact aagtagttat caaacgatag gatttccgat aaacacagcc tctttgatca
    31561 tgggagggct agtcatttta gggatgttta tctatccaaa aaaatgggcg aaacgaatgc
    31621 ctagttcgct gctggctatc attttggcaa cagcagttat gctattgttc catttaccag
    31681 tagcaactgt gggtaagatt ccacaaacat tgatcagtag taatcgtttg aatatgggag
    31741 attttagctt ttctgccttg caacaagtgg cagttccagc tattagcatt gctttattag
    31801 gaatgattga aagcttgcta tgtggtgctt cggctggccg aatggcaaat aagcccttag
    31861 atagcaatca agaattaatg gcgcaaggaa ttggaaatct gttgcttcct ttctttggcg
    31921 ggattcctgc tactgcggcc atcgcccgta caagcgtggc catcaaatct ggtgcacaaa
    31981 ctagattagc tggtatgata catgcagttg tcttactgct ctccatgctt gtttttgcgc
    32041 cagtgatgtc acagattcca atgccagcac ttgcaggtgt tttgattgtg acggcttggc
    32101 gtatgaatga atgggaaacg atcaaggagc tatttactaa taaatattgg tcagcagttc
    32161 tattgtttat ccttacgatg atttgtacag ttatctttga tttaagtatt gctatcgtga
    32221 tcggggtgat cggcggctgt gtattttttg ttgtcaaaag cgctgctatc acgatttcat
    32281 tagaaacgat cgattggcaa agaatggggc tgccagaaac ggaaaagctg aataactgga
    32341 cagtagtgta tattagtggt ccactgtttt ttatgtcttc agaaaaactc aaaaataaat
    32401 tggaagaact aaatcatcac gatggtgtca ttttctccat gcgaggcgta ccaagtattg
    32461 atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa agcagagcaa agaggacaaa
    32521 cgattatcta tacttccttg cagccagaag tagaaaaaca actttctcac ttgtgggaac
    32581 atacattcac gcatcaacat caaacagttg ctcatgcgtt gacagcttta catgcgaaat
    32641 atactgcaat tagccattaa aacaatgaaa aagaacagac aagactgtaa actgaagatt
    32701 ttatggtctt gtctgttctt tttaatattg ctgtaaaagt gaagctagta cttgccagtc
    32761 ttgatggata acaggtacaa gggagcgatt gtagaaaata gctgctggat gaaaaagtgg
    32821 aagaattcga tagttttttt ctgaccattc gtaggcatcc ttttgttctg ttaattgtaa
    32881 gacaggccct gtatagagtt gaccatgatt tttggtaacc gtatattctt tgccaagtaa
    32941 acgttgaaga ccaatgtttc caagtgtcgc aattaatttt ggctggacct tcatcagttc
    33001 gtaatccaat attggtgcat gcgcaaatac ttcggaacga gtaggtgtcc gatttggata
    33061 aacagtttct gactgttgtg tccgtttatt gatccgatga gtcacgcgat acgggcggct
    33121 ccttacagca ctggtgatat acacctcttc tcgtgttaag ccagttgaag aaagtgcctg
    33181 catcaattct tttccagctt gaccgctaaa aggaacatgc gtatcaatct ctgttttccc
    33241 tggggcttct cccacgagca tcagtttagg atgaagaggt ccttgcccag cgataaatcc
    33301 ttctacagga cggccgttca ttcttaaaga agcttcttgt atcagttctt ttgggtattc
    33361 catttatgct cctccttttt cttttattat aagaataaaa gaaacacaat gcgactattt
    33421 tatttggtag aactgtgagg atagaaaagc agaagtattg cttgaatttt tgttttactt
    33481 tttgatttta aatttagttg actaacggtt gctgttctgg taagctagta ctgttgtaat
    33541 tgtggactcc acagctacaa ccgctcagaa caagtaataa gcacagcata tgctgtcact
    33601 tcggatggcg agtctaagta tatcagaagg aggtgcttta tagcatgtac gcaattatta
    33661 aaactggtgg taaacaagta aaagttgaag tgggacaagc aatctatgtt gaaaaacttg
    33721 acgttgaagc aggagaaaaa gttgtttttg acgaagttat cttagtaggc ggagaatcta
    33781 caaaagtagg cgctccaact gttgcaggtg caactgttga aggtactgtt gaaaaacacg
    33841 gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa aaaacattca caccgtaaac
    33901 aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc gatcaacgct taatagcgtt
    33961 tgagaaagaa ggcctatata tgatcaaagg aacatttaag cgaaatgacg caggtcagat
    34021 cgtttcattc actttgaccg ggcatgctga tgctggtcct tatggaagtg atatcgtttg
    34081 tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg attgcctcat tagccggttt
    34141 tgagccgatt gttgaaatga atgaagaaga aggcggttat ctttataccg aagtcacttc
    34201 aggaatgaca caagaacaaa acaatattgc tgagattcta ttagaaaatc ttttattagg
    34261 attacaatcg atagaagctg aaaactcaga atatatccaa attaaaacaa ttaatgataa
    34321 ataggaggtg cagaccatgt tgttatctat gaatctacaa ttatttgccc acaaaaaagg
    34381 tggcggttct acatctaacg gccgtgactc agaatcaaaa cgtctaggtg ctaaacgtgc
    34441 tgatggacaa acagttacag gtggatcaat tctttaccgt caacgcggta caaaaatcta
    34501 tccaggtgta aacgtgggta tcggtggaga cgacacttta tttgcaaaag ttgatggcgt
    34561 agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg tctgtttatc cagtagctca
    34621 agaagcttaa taaataaact gctctatctc ttgtgtagca agggatggag cttttttgta
    34681 aattatggga ttttttttga ccatactttt gaccatactt tcttagaagt taatataatt
    34741 tgttagtttt tgtgttgttt cttctttttt gctgttagta atgtgtgtat atgtgtccat
    34801 agtagttttt atatttgcgt ggcccattct ttcttggact tctttgtgat ctgcgcctgc
    34861 ctcatatagc attgatgcat gtgtatgtct aaaaccatgc agaccgatat aaggtaagcc
    34921 ggcacgctca cgatatatat tgtatctttc tgccactgac tgattaattg tgaaattatt
    34981 ttcgtcattg gtaaacaaat aattagcttt agtaaatcct aattgaaata agtactttct
    35041 ttgttcgagc ttccaaaatt ttagttgctt tatagttttt tcatctaaat aaattattcg
    35101 atttgatttt ttggttttag gagtttcaga tatataatat ttatctgaac gagctacagt
    35161 ttttttaatg gccatttcat ttgttttaaa attaatattg tcccaagtga gtgctaatat
    35221 ttcgccgatt ctacatccgc tgaatgctaa tagtctaaag agcgtatagt ctctgttttt
    35281 aaaatatgga ttctgttctt gagatacttc atttagaaat aattccaact gttcttttgt
    35341 atataatttt aatttctttt ctgttttaat ttgtggcttt ggaataagta aatttaatgt
    35401 tggattatca gatgttaaac caatatttat agcatactta aaaactttat tcatatagtt
    35461 tatgaaaaga gggtactgct ttgaagttcc tttttcactc caatcattta ctaccttttg
    35521 acaatatgcc gtattaattt tagaaagctt aatatttcca aatttgggat atatatgttt
    35581 attaaagatt attttagtcc ttgaatatga actttctttt acagtttttt tgtagctttc
    35641 tagccataat tctgctgctt ctttgaaagt agtatttgta cttgtgggca ttcctgtttt
    35701 ttgtaattct agttctaacc tagaaagtgc tgtttttgct tctttttgtg ttctaaatcc
    35761 tcgtttagta gtatattttt tctttccagt tagcggatca ataccgagat aagttttaaa
    35821 gtaccatgct ttttcgccat ttttcttttt atattgtttt atcattgcca tcaatattac
    35881 ccctattcaa attctaattc tgccatgcta tcattgaaaa attcgatcag tccttgttcc
    35941 tctataaatt caagccatat ttccttcccg ttaattaatg gaactccttt ttctttacga
    36001 attgtttgat acaagttagt cacgacttgt ttccacattt ctaatacagc tttatctatt
    36061 ttcttatcat cccagcgttt tttttggggt ctatttttgt ttttttcaat ttgataatta
    36121 gtagctttga tgataaatgt ttcgtatccg gttgtgcttt tttctaacca ttctttgaat
    36181 tctgtatata tcatttttgt tcacctttct atttttgaac gtatgttctt ttttgcttaa
    36241 aaaattttag agagctgcat aacaatagat gcgagctttt aataattctt tcacaagtgg
    36301 ttcgaagcga acgcttaaat tgttatcttc tataaatttc atgtaattga tattgtgtgg
    36361 ttctaattca gactttgcaa tatatctatc taataatttt tcaatcatga agcgatcagc
    36421 ctcatattcc atttttgaat ggagagagaa tgctaagtta tataaaaaat aattatcatg
    36481 atgtttagaa gcgtgtccta attcatgtaa taaggttctt tgtcttctat acttagataa
    36541 attagctttt attactatgg tattcataca agcaacataa tggccatcgg catcaaggtt
    36601 ttcacgctct tctaccttaa cgccgagttc attaattatc atttcaattt gactgtccaa
    36661 atgactcacc tacttattta gttatctttt ttctctaaat atgcttcaat catagctgat
    36721 agtatttccc tatcattatc ggtgagcggt tttccgtcgc tactcataac agatgctaaa
    36781 gcttcttcca cggttagttt tctttcctct ggtgcaactc ccgcgttagg atcatctgtg
    36841 cgtcctagca aataatctgt tgatacatgg aagtagtcag caactttttg caaagtttct
    36901 attttaggac tttgtgtctt ccatttataa atagagtttt ttccaaatcc tattctattt
    36961 tctaattcag aaatagaaat acctcttttt ttacagagct ctttaaccct ttcgaaaagc
    37021 atgtcaaatc aaccttccta gagcgtacga taataattta tacaaaaaga tagaaaggta
    37081 gttgacaaat ctatccaatt gtcttatatt taactcgtaa gctaatttaa taagctaaca
    37141 agcaacaaga aatacaccta ataaaataaa cgaattcgag gtcggcaaac taagaatgtt
    37201 aatttttagg gtttttctag gcttatttaa ctgtatataa atataagaca gttggataga
    37261 aatgtcaata aaaattagct aattttatta gcttacatat ttaaagaaaa ggaggaaaca
    37321 tcatgaaaca aaataaaaaa actccttccg caaaaggagc tttaataact ttaaatttta
    37381 aagaccaaaa gtttagtgct gttgttgatg aaattcatcc acaagagcat caaaaaaatt
    37441 cttttgcaac tgacgagaaa aagactcaga aacgctctga gaaagttctt cgaataattc
    37501 aatttctgtt ggaaaagatt cgtcattttg gaagtctatt ttgaattctt tattaacagt
    37561 cttgaatacg gtttgtttga ataaggcgtc gacatccata ttaaattctt ccatactatt
    37621 ttcacctcgc tttctattca aaattatatc aaagaaagga atgagcaaaa atgaacacac
    37681 cgcaaatttt taactttgaa caacatgaag taagaacagt aacaatacat gatgaaccat
    37741 tttttgttgg aaaagatgtg gctaaagttc tagggtacca aaatggtagt agagatatta
    37801 acagacacgt cgatgtagaa gatcggcaaa actaccaaaa cggtactttt gaatcgccta
    37861 gagggttaac tattattaac gaatcaggtt tgtactcctt aattttagga agcaaacaac
    37921 caaatgctaa gaaatttaaa cgttgggtga ctagcgaagt gttgccagca attagaaaac
    37981 atggaggtta tctaactcca gaaaaagtag aagaagcttt gcttaatcca gatacaatca
    38041 tccaattagc aactaagttg aaagaagaac gtactggaag actaatcgca gaacaaaaaa
    38101 ttgcagaata cgaacccaaa atttcgtatt tggatagtat cttatcttct acagattcag
    38161 taactattag tcaaatcgcg gctgattatg ggatgtctcc acaacagatg aacaaactac
    38221 ttcataagct aggtattcag aaaaaagtag gcaaccaatg gttattatgc aaaaaacaca
    38281 tgagacaggg ttacacaaaa tctcatacaa ctgagattcc gaaatctgat ggcggaacaa
    38341 aagttgtgat gaatacaaaa tggactcaaa aagggcgtct gttcatttat gagtcgctga
    38401 aaaaagaagg atatattcca gaaatcgatt tattagagga ggaatgacga tgtcacaaga
    38461 atttattttg aaagtcagaa ttcaattagc aaaatacgga aagtctcaga actggttagc
    38521 tgatactatc ggaatttcta gaccatatat gtcggacatt atgaatgggc gtagaaaacc
    38581 agataaacaa atcaagccaa ttgaggcagc actagcagag ttagagaagg agaaataaca
    38641 tgcaaataac aattccagat aatttagtag tttccgaatt aactacacag attacgaatg
    38701 ctgttcttaa ttcattggac gaacgattac accttatgaa caaatcagta gagcttcctc
    38761 catatccaaa caaatcagag gtaaaaaaag ttttaggcat tggtgatgac aaattaacac
    38821 attggataag cctaggctta aaaacacagc agtggagcaa gttagacatc agaattgaac
    38881 gatcggaact ccaaagattt ttgaaagaaa actttgagtt ctaaaggcaa aggagaatga
    38941 ttttatgtcc tacacattgc aacaagaaca tcaaattcta ggtctgatta aacagcgtag
    39001 aaaacaatta caagatgatc gtgcagcgct tagaaaatcc gatgagctat cagatagaca
    39061 agctgaacta attgcttctg aacttgagga tttgagaatg ctagaaataa aaaataggga
    39121 gattagatta tgaagaagac agacacactt tttataggat tcattttggg gttattagtg
    39181 attgtagcgc accaaagtat tatcggagga agcttgttcg cagcattgat ggttttaatc
    39241 aatctgcttg actcaaaaga aaggagcaac tatggcacga gaagaagcgc taaaaatcgg
    39301 taaagtgatt gctgataatt ggtgggcaaa tagccgttct attattttaa gcaagcaaca
    39361 tatcgaaaag aaaaaagcat ggcaacaaat aaaaaagtga ctccgccgac caaagcaatg
    39421 agtcactcaa aaaatcataa ctaaggagat tttaacatat ggaaaatgaa ctttccactc
    39481 tagatcaata tttggccgat cctgattggg gcaaatcgaa tttacaactg gttgctaaca
    39541 agtacgaaaa atcaattgat gaagcaattg ttgcattgac tgatgcagct tccataaccg
    39601 ctgatgacag taaatggacc aagagagact tgggcattct aaaacgaggc gttaattggc
    39661 ttgaagatca gtacagagaa gaaacaaaag agaagtgata tgagtgttta aaccactaat
    39721 agattcatac tcggcggtac taaagaaatt taaaggtaac gacattggtg caacaatcaa
    39781 cgaagaagta aatatcgaac ggctgaaaac gatgtatgac ggatatgatg gcgatcgagt
    39841 cattgaaatt cgctttattg atccacgacg gttcacagta cagcaaagaa acttcatcta
    39901 tgcgctcata ggcgatattt tcatcgatac aggcatgcca acggacttct ggaaggaatt
    39961 cttctacttc cgttttgaag gtgtcacagg gcgcgaaata agcctcaaag acgaatcgaa
    40021 tacaaccgtg agtgatgcca atatcttagc gaatatcatc ctagatttca tctttgaaca
    40081 tcatattcct ttcaaagaag gatatgagat tttaccagcg aatcaagagt attacttcta
    40141 caaatgcatt acaaaaagag tctgctgcat ctgtggcaaa acaggagctg atatcgatca
    40201 ctttgacaaa gcgct
//
LOCUS       DACEPW010000006        36653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEPW010000006 DACEPW010000000
VERSION     DACEPW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.006"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            257..742
                     /locus_tag="IW819_000238"
     CDS             257..742
                     /locus_tag="IW819_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1243373.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            735..1418
                     /locus_tag="IW819_000239"
     CDS             735..1418
                     /locus_tag="IW819_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1243374.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1544..4189
                     /gene="polA"
                     /locus_tag="IW819_000240"
     CDS             1544..4189
                     /gene="polA"
                     /locus_tag="IW819_000240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1243375.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4234..5070
                     /gene="mutM"
                     /locus_tag="IW819_000241"
     CDS             4234..5070
                     /gene="mutM"
                     /locus_tag="IW819_000241"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1243376.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            5034..5663
                     /locus_tag="IW819_000242"
     CDS             5034..5663
                     /locus_tag="IW819_000242"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1243377.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5715..6047
                     /locus_tag="IW819_000243"
     CDS             5715..6047
                     /locus_tag="IW819_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1243378.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6359..6850
                     /gene="nrdR"
                     /locus_tag="IW819_000244"
     CDS             6359..6850
                     /gene="nrdR"
                     /locus_tag="IW819_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1243379.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6873..8246
                     /locus_tag="IW819_000245"
     CDS             6873..8246
                     /locus_tag="IW819_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1243380.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8246..9172
                     /gene="dnaI"
                     /locus_tag="IW819_000246"
     CDS             8246..9172
                     /gene="dnaI"
                     /locus_tag="IW819_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1243381.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9301..9741
                     /locus_tag="IW819_000247"
     CDS             9301..9741
                     /locus_tag="IW819_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1243382.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            9885..10586
                     /locus_tag="IW819_000248"
     CDS             9885..10586
                     /locus_tag="IW819_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1243383.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            10583..11704
                     /locus_tag="IW819_000249"
     CDS             10583..11704
                     /locus_tag="IW819_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1243384.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            11719..12951
                     /gene="pepT"
                     /locus_tag="IW819_000250"
     CDS             11719..12951
                     /gene="pepT"
                     /locus_tag="IW819_000250"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1243385.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            13083..13991
                     /locus_tag="IW819_000251"
     CDS             13083..13991
                     /locus_tag="IW819_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1243386.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            14026..14298
                     /locus_tag="IW819_000252"
     CDS             14026..14298
                     /locus_tag="IW819_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1243387.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            14309..15355
                     /locus_tag="IW819_000253"
     CDS             14309..15355
                     /locus_tag="IW819_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1243388.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            15606..18215
                     /gene="clpB"
                     /locus_tag="IW819_000254"
     CDS             15606..18215
                     /gene="clpB"
                     /locus_tag="IW819_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1243389.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(18366..19037)
                     /locus_tag="IW819_000255"
     CDS             complement(18366..19037)
                     /locus_tag="IW819_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243390.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(19030..19524)
                     /locus_tag="IW819_000256"
     CDS             complement(19030..19524)
                     /locus_tag="IW819_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1243391.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(19544..20764)
                     /locus_tag="IW819_000257"
     CDS             complement(19544..20764)
                     /locus_tag="IW819_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1243392.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            20911..22746
                     /gene="lepA"
                     /locus_tag="IW819_000258"
     CDS             20911..22746
                     /gene="lepA"
                     /locus_tag="IW819_000258"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1243393.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            22943..23854
                     /locus_tag="IW819_000259"
     CDS             22943..23854
                     /locus_tag="IW819_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1243394.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEISKESNRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFNKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            24852..25103
                     /locus_tag="IW819_000260"
     CDS             24852..25103
                     /locus_tag="IW819_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243395.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFVESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            25186..25458
                     /locus_tag="IW819_000261"
     CDS             25186..25458
                     /locus_tag="IW819_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243396.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            25548..25787
                     /locus_tag="IW819_000262"
     CDS             25548..25787
                     /locus_tag="IW819_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243397.1"
                     /translation="MIDISIPEDLKPDPATIGNSGMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMIFGDGEVANKAGIPIGKWTVDFHEAGSFFVL"
     gene            25934..30229
                     /locus_tag="IW819_000263"
     CDS             25934..30229
                     /locus_tag="IW819_000263"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ1243398.1"
                     /translation="MYAKTKMNWLKALALVALSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKLNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KNMNGKVQAEIKDLDPGKYVLKEIKAPKGYALSDETIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKIANKDLVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKERYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVT
                     FTLYNGKGQAIGKYTTDQNGEILVKGLVANNYYFTENVPLESFQPNTGKQAFTITFRQ
                     NAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQEGKEITSILTFIADKANGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAVHVDINDDNQSVRVNNPTVHTTAVFEDGLTIADPTGLFTLNDTVEINE
                     ILPGKAYLVKGILMDKATGEALLVHGQPVESAVGFIAKAENETVIVPFTFDLTGLNGR
                     EMVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLIVGKTYTVNGVLMDKVTEKPVLVDGKEVSSTLTFVAETTDGTVNLDFILD
                     AHALRSKEVVVFEDLFREGVLLASHADITDKDQTIKIVSPTITTKATNANGGKEILAL
                     PNQKVVEWVQVNGLAIGQIYKLIVQGYLTPDGTPFEGTHAEKVFMADKEMMEFLFEFI
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQFVEAPKPPQPVK
                     PNEPQPVKEVKQAAMVPMLPKTGSEDNVLWIMIGMIFVLAAGLIGYDLYKVRD"
     gene            31147..31401
                     /locus_tag="IW819_000264"
     CDS             31147..31401
                     /locus_tag="IW819_000264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243399.1"
                     /translation="MEKELLLGFYRKENNGGKKNMEKKNINICFLSGKECTYEECTDI
                     SVHERDGMLIVDMSPSDEPFVVKHYFPLCNIESFRIDCEF"
     gene            31418..31783
                     /locus_tag="IW819_000265"
     CDS             31418..31783
                     /locus_tag="IW819_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243400.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            31785..32237
                     /locus_tag="IW819_000266"
     CDS             31785..32237
                     /locus_tag="IW819_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243401.1"
                     /translation="MGLKFPNNYDITEALDETCIGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNSNPKQSQEKGLDQKQPSADSKEKEKK"
     gene            32303..32659
                     /locus_tag="IW819_000267"
     CDS             32303..32659
                     /locus_tag="IW819_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243402.1"
                     /translation="MNLDKTNQTLREIMVCCQNIQQLIEYFGDNPTIFIQTNEYICAV
                     IGQCMFLRDLVRFSLPYQWKQDHQEVVWIRVVRICNMCAHPYKNRLNLFRLWFFIKCD
                     IPVLVAFCQKFLAQEK"
     gene            32849..34042
                     /locus_tag="IW819_000268"
                     /pseudo
     CDS             32849..34042
                     /locus_tag="IW819_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            34169..34483
                     /locus_tag="IW819_000269"
     CDS             34169..34483
                     /locus_tag="IW819_000269"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243403.1"
                     /translation="MGEVLYRVSSAAGEISPDFAVRRLYEWINKVEYYTKGSYVFRRI
                     ERETLFVTRNQIVLTKEDILRFRQVYRLCKEKNIQLRLAILQCFAPEQYKELQEKEDS
                     LV"
     gene            34846..36102
                     /locus_tag="IW819_000270"
     CDS             34846..36102
                     /locus_tag="IW819_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1243404.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKKKIPEDFEKRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKIVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKQTVTQNVLVQFSGQGCREYEEVMREQRTD
                     WRTWLYSIWKNQGKLEAGYKRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKKFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISEIEAL
                     TKYGIYNRYELRLANEKATQAVEAFILDHSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIENAELNDEQQSYLEWMGTSGGKIA"
     gene            36099..36644
                     /locus_tag="IW819_000271"
     CDS             36099..36644
                     /locus_tag="IW819_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243405.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGTLKFIRFYRFSLPVPAILAVK
                     KGCDKMKIYENCVAALSNEKPLVEEISLATVTWEMNRQPTEEQTYLSLEEIELELEEI
                     GRYATCSTYCGSVISIAGVIV"
BASE COUNT        12428 a         6061 c         7859 g        10305 t
ORIGIN      
        1 aatcttgcca agcccccttt tttgcaactt atgataaacg gtgttcaaaa gcttgcatct
       61 gcggtaataa gcccaatcaa atcaaaaaca tgagaagctg ttttttcttg gctgttctta
      121 ttatttgatg caggacagct ttttcacgac ctctgtaaac tataggaatc tgttaaatca
      181 agggagcaga acttgccttt tacgctactt ttggtacaat aatgtatgat taaaaataaa
      241 ctggagggtt tctatcttga acataggtaa gccgagaaaa ctaggaaaaa caataaatga
      301 aatcgaggaa ggcgactcgt tatccttaac agaatctatt gaagataacc agcttttgct
      361 gtatttggga ttgacgaatg atgcaaatcc tttgtatatc caacatgact ttgcacaaaa
      421 aacagaatat caacagccaa tcgttccatc agtgatgctg atggggatca tcacaagtgc
      481 aatttcaaag catcttcctg gaccaggatc acatgttgtc aatttttctg tcaattttat
      541 tgaaccggtt ttccattatg aaacactgac ttttcagttt gaggtcataa aagtagataa
      601 gatgaaggat gtggtgacga tttctgtaga agcggagaat acgaacgaag atcgtgtgtt
      661 ggatgctgtc gttatggtcc aaccgccaca agttcctgaa ttggaaatag caatcgaaag
      721 tgaaggtgaa ttgaatgaat aatccaagta catcaatgtc aggtatcaat cgtttttatg
      781 caaaagttta tggattttta ggtatgggac ttgcccttag tgcagtcact tcttacttgg
      841 ctctttgggt atttcctttg caattagcaa cttttatcaa taattttcca ctaggctttg
      901 tgggtctgtg gatcgttgaa ctgattttag taatggttct aggcgtaaaa gcacaaaaga
      961 atccgagctt agcaatcggt ggatttattg cctactctct tttgaatggc ataacattag
     1021 cggttactct tgcatactac gatattggaa cagtaacgca agctttcttg acagctactt
     1081 tgatgttcat ggggatgtct gttgtcggag taacaacaaa aagagatctt agcggggttg
     1141 gaagagcagg acaggcagct ttaatcggag tgatcattgc actagtcttg aacatcttca
     1201 ttttgagaag tagcgtagtt gagctgttca tctccctact gttagtcgtc atctttgcag
     1261 ggattacagc ttatgataac caaagcatcc gtaatatcta tgtccagaca aacggaacag
     1321 cacatagtgg aattgctgta ttcatggcat tgaagctgta tttggacttt atcaacttgt
     1381 tcttggcgtt tctgagaatc tttggacgga acaactaaaa aagaaaaaaa ttcatcagtt
     1441 ggggctatac cgaattattt ggtatggtcc cgctttttct tttcgcattc cgcaggattc
     1501 tttgttagaa tgaataagaa ctttacagta aggagttaag aagatgacaa aaaataaatt
     1561 attactcgtt gatgggaaca gtgtggcttt tcgcgcattc tttgcgcttc acaattctct
     1621 tgaacgtttc aaaaatagaa acggtcttca tacaaatgca atctatgcat tcaatacgat
     1681 gtttgaaaat gttatgcaaa aagagcaacc gactcatgtt ctggttgcgt tcgatgcagg
     1741 aaaaacgact tttcggacag aaatgtatgc ggagtacaaa ggtggaagat ccaaaacacc
     1801 tggcgaattc aaagaacaaa tgccatatat acgtgatttg ttgacaggac taggggtaca
     1861 gtattacgaa ttgcccaatt atgaagcaga tgatattatc ggtactttgg cagaaaaagt
     1921 agacaaagat caatttgatg tcgtcgtgct ttcaggtgat cgggatttga ctcaattagc
     1981 tagtaaagaa gtaaaagtgg atatcactgt aaaaggtgtc agcgatatcg aaagctatac
     2041 accagaacat gtagcggaaa aatatgatgg gttgacgcca aaacagatta ttgacatgaa
     2101 gggactagcc ggagatgcct ctgacaatat ccccggtgtg actaaaatcg gtgaaaaaac
     2161 agctatcaag ctattgaaac aatatggctc agtcgaagga atttatgaac atatcgatga
     2221 aatgaagcaa agcaaaatga aagaaaatct gataaatgac aaagagcagg ctttcttatc
     2281 gaaaaaattg gcgaccatca atacttcagc gcctgtggat gtctctatcg actcattgaa
     2341 gtatgaaggg aaaaatctcg ataaactcgt tccgttttat aaagaaatgg atttcaatca
     2401 gttcctttct aaattgaata tcgtagaaga agaagtaaaa atggatgata ttttgtttga
     2461 agtcgttcat gaattcaaag aggaaatgtt tactacggat atggcgttgt acgtggaaat
     2521 gatgggcgac aattatcata cagaggaaat cgtcggagta gcgtggggga cagaaaagaa
     2581 aatttatgtc acgaacgatc tatctgtatt cgaaaaccga gcgttccatt cttggatcac
     2641 tgatccgact cggctgaaaa aagtatatga tgcgaaacga acttatgttg ctttaaaccg
     2701 ctatactgga aaaacgaaag gaatcgattt tgatgtcttg ctaggtgcat accttctcga
     2761 tacgaatgaa aaaagcacag atatcgaagg aatagctgct cattacggat acaacgatat
     2821 ccaatcagat gaagctgtat acggcaaagg ggcgaaaaaa ggtttaccag aagaagagga
     2881 actctttttt gctcatttag cgcgaaaagt agctgcaatc aatgcgttga caccaaaatt
     2941 gagccaagaa ctggcagata aaaaccaaga agatcttttc cggaaaatgg aactgccatt
     3001 gtctatcatt ttggcagaga tggaaataag cgggatcaaa gtagatgcga ctcgcttaca
     3061 agaaatgaag ggcgagttct ctgctcgctt aagagagatc gaacaaaaaa tctacgaaga
     3121 agccggcgaa gaattcaatt tgaattcacc aaagcaatta ggagtgatct tatttgaaaa
     3181 aatgggatta cccgttatca aaaaaacaaa aactggatat tccacagccg tagatgtact
     3241 ggaacagctt cgtgaacaag caccaatcgt agaggatatc ctcacctatc gtcaaattgc
     3301 aaaaattcaa tctacctatg tcgagggact gctgaaagtg atccaatcag atggaaaagt
     3361 gcatacccgt tatgtccaga cattgaccca aacaggacga ttaagctctg ttgatcctaa
     3421 tttgcagaac atcccgattc gattagatga agggagaaag atacgtcagg catttgttcc
     3481 tagagaaaaa gactggctga tttattcttc tgactattca caaatcgagt tacgcgtact
     3541 tgcgcatatc tcaaatgatg aacatttgaa agctgccttc ctagaaggtc aagatatcca
     3601 tgccagcaca gcgatgcgtg tatttggaat cgaaaaggca gaggatgtca caccaaatat
     3661 gcgtaggcag gcaaaagcag tgaattttgg tatcgtgtat ggcatctctg attacggact
     3721 ttcacaaaat ctaggaatca gccgaaaagc agcacagcaa tacatcgata cgtactttga
     3781 aaaataccca ggtgtcaaag aatatatgga aagaatcgta cgcgaagcaa aagaccaagg
     3841 ctacgtcgaa acgctgtatc accgccgtcg atatctgcca gacatcaatt caagaaatta
     3901 caatatccgt tcttttgcgg aacgaacagc tatcaataca ccaatccaag gaagtgctgc
     3961 tgatattttg aaaatcgcga tgatcgaatt ggataaacga ttaaaagaaa caggtcttca
     4021 agctacgatg ctgttacaag ttcatgatga acttgtcttc gaggtaccgg aaaaagaact
     4081 tgaatcatta gataaattgg tgaaggaagt tatggaacaa gctgtttctc ttcatgtacc
     4141 actgatcact gacagcagtt ggggaaaaac gtggtacgaa gcaaaataaa cattgtttaa
     4201 gacagagcaa tataatgaag gtgaaaagaa aaaatgccag aattaccaga agtagaaacc
     4261 gttcgtaaag gattggaacg gttagtagta ggaaaaacga ttcaaaaagt tcaggtcttg
     4321 tggcccaaga tcatcgagca accagagaca cccatctttg aggcttctct tgttggcgaa
     4381 accatccaat ccattggacg tagagggaag tttttaattt ttcatctcga tcattgtgaa
     4441 ctgatctcac acttgcgaat ggaaggaaaa tatcaattta cgaaagaaaa tacaccaatc
     4501 gataaacata cgcatgtact tttctttttt gaggatggaa gccaacttcg ctacaacgat
     4561 gtacgaaaat ttggtcgtat gacaatcgtc gaaaaaggag caagtgcaac ttaccgagga
     4621 atcatgaaat tgggacctga gcctttgcca gatagcttct tattagcaga tttcgcaaat
     4681 ggactgaaaa agtcccacaa agcaatcaag ccattgctgc tagaccaacg tctagtgact
     4741 ggtctaggta atatttatgt agatgaagca ttatgggaag cgaagatcca tccagaacaa
     4801 ccggcgaaca cactgcgccc caaagaggta gaacaattac gtctatcgat cattgatgtt
     4861 ttggatcgtg caatagaagc cggaggtacc acgattcgca gttatttgaa tgcattagga
     4921 gaatcaggcg gatttcaagt agcactgcat gtctatcagc aaactggcaa accatgtata
     4981 cgttgtggaa cgccgatcgt gaaaacaaaa gtggcacaaa gggggacgca ctattgtccg
     5041 aattgtcaaa ggctaaaaat cgcaaagtag gatttgtttt aggtttgacg ggtggaatag
     5101 ctaccggtaa aagtacggca gccaaagtct ttcagtctca tggatttcct ttgatcgatg
     5161 gcgatgtgat tgccagagaa actgttgagc ctggaacgct tgctctgagg aaaattgtat
     5221 cagtgtttgg ccaagaggta ttgcagcctg atgggcaact agatcgaggg aaacttggga
     5281 tgattgtttt tccatcaaag gaaaaacggc aaaaacttga tcagctatta gatccgttta
     5341 tccgaaaagc gataaaagat caaatcgcct ctctaagttc caatcatcct ttagtaattg
     5401 tagatatccc attgctctac gaaggtcatt atgaccacta tatggatgca gtagctgtcg
     5461 tttatactac gccagaaact cagcttcagc gtttgatgaa acgtaatcaa ctgacgctag
     5521 aacaagcgca gcagagaatt agtagtcaac ttccaatcga agaaaaaaag cagcgtgcgg
     5581 atattttgtt cgacaacaat ggaacaaaag aaaaacttgt gggacaaatc gaaaattggc
     5641 tttttgctaa tcgctttctt tgagtatctg tgtcaatttt gctatcctga gtatatagaa
     5701 agtgagggat agcgatggga tcattaaaaa atcaaaagga taaagtcgta ggtaacgcaa
     5761 aagaaaaaat cggcgagatg ctcaataaag aaaatctaat cgatgaaggt cgtacgcaat
     5821 cagaaacagc agaagaaaaa gaaaagcagt accaagcaag aatcgatgaa atagaagacc
     5881 agcaggcaga agaagaagcg aagcggcaag atttgactca agaacgaaaa aatccgacac
     5941 ctgatcgtag taaaacaccg ctcccagaaa gtgaccacat ttccgatgag caaaaaatcg
     6001 aggaacagcg aatgaaacac tatacaggaa tcgaagagaa gttataaaat agtaaaaaat
     6061 cgagatgtga cttgaagcag atgagaaggt gttcatctgc ttcaagtttt ttgtgctaga
     6121 tgactaaatc agcttttgtg taagttattt ctcagtaatc aaggtgatca gtattggaaa
     6181 attgaaattt agtaaggaca ggggacacaa ttacgcatga aagtagaaaa aattgttctt
     6241 atatccgtgt tttttgggta ggacagtcgc gtttgtgatt tgtttcgtgt tataatacaa
     6301 ctagttgtaa agaaagacac atcctttata taaaatgcga tgcaagaggt gagacaaaat
     6361 gagatgtccc agatgtcaac ataataattc acgagtaatc gacagccgtc aagcagatga
     6421 tggacgtgcg atccgtcgcc gtcgcgaatg tgagaattgt tcgttccgat tcactacatt
     6481 tgaacgaatt gaagctgctc cgttattagt aatcaagaaa aacggggaac gcgaagagtt
     6541 caatcgcgat aaaattttaa gaggactcat ccgttcagca gaaaaaagac ctgtagctat
     6601 ggaacaaatg gaacaaatcg tcaaccatgt cgaaaaccgc gtacgcagtc tcggagaaaa
     6661 cgaagtatcg acgaatctaa ttggtgaata tgtgatggaa gatttagttg atttagacga
     6721 aatcgcttat attcgttttg caagtgttta tcgccagttc aaagatatgt ctgttttctt
     6781 aaaagagttg caagatatcg tggataaagc aaaagaaaat aataaagaga ataataaaga
     6841 aaacaattaa gaaacaaaaa ggaggcattt ccttggaaaa tgcttggaaa gagcttcaac
     6901 caaaaaatat ttaccaagga agaaaagcgg ctccgctgag agaagacagc aagaacgcct
     6961 tgctgtatct ttatcagcca ctaatcggcg gagaagcctt gagtatctat ttcaccctgt
     7021 tatcagaaat ctctcaagaa tctggcaaag gtcctgaagg gatgcatgca gatctgctta
     7081 gtagtctagg ctgcggtatc ccgcaatttt atgatgcaag aaaaaagcta gaaggaatcg
     7141 gcttactgga tgtttatttt aaagaagata gtgatctggg acctcgtttt atttatgagc
     7201 tgttagaacc actcattcct aaagcatttt tcaaagactc tgttctggct tttcttcttc
     7261 tggaaaaagt tggtgaacga cgattccaga agcttaccca attctttgaa cctaaaaggc
     7321 atacagcaga gggatatcaa aaaatcacaa aaaaattttt agatgtttat cgcttcaatg
     7381 aagcagtgta cgctgctaat caagaaaaca tcgaatcgct gaataaatct tttgaagaat
     7441 ctgaatcagt agcattactt gaagcagatt cgacattcga ttggcagttt ttgaatgatt
     7501 ggctggcaaa aaaacatatt gcaagtcttg atgatccaat catcaaacaa gtcgacatgt
     7561 atcatcagct ttacggtttc gatgaattga cacttggcga actgatcagt caatcttatg
     7621 actttagtga acaaaaagta tctgaaaaag aactgcaaaa agccattttg gcaatgcaga
     7681 gacgtccggt gaagcaaaca aaagaaacag ttgcggtttc tgaaaacagt aatgaaccct
     7741 cgtcaaacga acatttgaca aacgcagcgc ttcagctgat aaaagaagca cagagcattc
     7801 caccgatgaa atatctggaa gcaatcaagt cagaaaaagg cggatatgtt tctaaacaag
     7861 aaaactggtt actgcaagat cttgtttctc aatcaggtct ttccagtagc gtgatcaatg
     7921 ttttgctgaa ttatgtacta gtgatcaaga ataatgctag cttaaatgcc agttatgtaa
     7981 atacagtggc aaatgaatgg gcacaaaaga aaatcgccac agcagaagaa gcgatccagc
     8041 acattcggca aataagtaat caagcaaaac aatcaaaaca aaagaagcaa acaaattatc
     8101 aaactggcaa gcgaaatgtt cgacgggaaa agcttcctga ttgggtcaac cagccaaaag
     8161 atgaaaaaca gatcagcagc gagaaaaaag cagagattga ccgccgtttt aaagaatacc
     8221 tggcaaaaaa ggagggtgat atctagtgga agacgtaggc aaagaattga agaggatcat
     8281 cagcaaacgc aattatcaag aaaagtatgc tgaaatgatc gctgaagtct taaatgatgc
     8341 ggatgttcaa gcttttctaa gcgcacacca agaagaattg acacaagcgg atattgaaaa
     8401 aagttacgct aaattgtatg aattcgtgca agaaaagcgt aaatatcaaa aaaacgatcc
     8461 aacgatgatc gctcccggct atgagccgaa gctgacgttg aattttcatt atgtggatgt
     8521 cacttatgta ccaacggaag cattgttagc acggcaaaga caagaagaaa tcagaagccg
     8581 agtaaaagca atggatatgc caaaagatat tcaatcggct tcttttgaaa actttgaacg
     8641 tacagatgga cgaggatatg cgagcttaga agccctagat tttgtagaga aatatactgc
     8701 tgatccaaaa gggtatcata aaggactgta tctagtagga agttttggta ttggaaaaac
     8761 atatttatta ggagcaacag cgagagaatt agcgattgct ggattcacca cgacgctagt
     8821 ccattttccg acatttgccg ttgagatgaa acaggcaatc ggaaaagatc aagtggcaga
     8881 aaagttagat gcagtgaaac gttcacctat tttgatgatc gatgacattg gtgcagatgc
     8941 gatgagtagt tggatacgag acgaagtatt cggtgttatc cttcaatatc ggatgcagga
     9001 acagttacca accttcttct catcgaattt tacaatggat gaattggaac agcatttgtc
     9061 cgtcactcaa cgtggagatg aagagccgct aaaagcgaaa cggataatgg aacgcatacg
     9121 ttatttgaca aaagaaatcg aaatgactgg tcgcaaccga cgcaatggtt gaacagttga
     9181 caagtccaac tgacatttga cctagtagta aaagcaggtc agcgaatcat tatatggaca
     9241 gaaatgaccg gagacaatca ccgcaacaag taatgcagaa acttaaacgg aggatttttt
     9301 atgagggcta ttttttctag tatatctgta gttggtctat tttttctttt agcaggctgc
     9361 ggtagcgcag atagcaacac aggttctgtt gattcgattg atgcgggtca aacagtaaaa
     9421 acaagcagta cgaaaacgga gagacaaagc atatttgtcg gaacgattga aagtattact
     9481 caagtagaag cagaagaaga aacgcttcag ttgaaactcg tcgatgtcat ggccaaagaa
     9541 gatccagcga atatgggaac gtcattcagc aatgacggag tgatcttaaa tgctactgct
     9601 gaaaaaataa gcgtggacat aaatgaattg aaaatcggtg acaaagtcca atttacatta
     9661 gttgagatgc cgatcatgac gatgtctatc ccaccgcaag ttcctggaga ttcgattatt
     9721 gatgtttcat tgaacaaata accaataaca ttttttcaaa aataagaaaa tgttgtttta
     9781 tagaacgaaa aggtatattt tttaaggaga tatgggaatt tttttattcc tagtctcctt
     9841 attttatttg gaaaatgcta taattatcta gaaggagtga agctatgaac catttagatt
     9901 tatctcagcg cttagccgca gtgggtgact ttgtccctga atccgctcgt ttagcagaca
     9961 ttggatcaga tcacgcttac ttacccgttg cattgatatt aagaggaaaa atcgaatatg
    10021 cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc acgtaagcaa gtcaaaaaag
    10081 atggattaga agaccgtatc accgtacgcc ttgcaaatgg tctagatgct atccagccac
    10141 aagatggtat tacagctgtc acgattgcgg gtatgggagg ttcactgatc cgagatatcc
    10201 tagaagctgg caagttgaat caacgcttaa gcggacaaga acgtttgatc cttcagccaa
    10261 acatcggaga gcgtacatta cgatcttggc tgcaagaaaa tcagtataaa atccaatcag
    10321 aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat tgtagccgaa aaagaacaaa
    10381 aaccagttac ttacaatgaa atggagctga tgttcgggcc atttctgctt gaagagaaaa
    10441 gtgagatctt caaatcaaaa tggacaagag agcttcaaca aagaaaagct atccgtgaac
    10501 agctcaaaca agcagctaat caagaggcac gtcaaaaaca aatcgagcag gaaatccaaa
    10561 tgattgagga ggttttaaag ccatgacaat cagcggacgc actttcatcc aaaagtttga
    10621 aagctattgt cctcagtggc tagctgaaga aggagaccca gtcggactgc atatcggaac
    10681 attggataag ccaatccaac gagtaatgat gacattagac gttcgtccag aagtggtcga
    10741 agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa catcctccaa tctttcgtcc
    10801 tgtaaaacgg ctggttactg atcagccaca agaaaaaatg tatgcagatt tgttgaaaca
    10861 tgatattgcc gtctatgcag cacacacgaa catggatatt atttgggatg gactgaatga
    10921 ctggttttgt gaacttctcg gtattgaagt ggagagttat ttagtaaaga ctcatgagat
    10981 tcattataaa aaactggccg tttatgtacc tgtggatcat gcacagaaaa tgcgtgaagt
    11041 attagctgca gctggtgctg gtacacaagg tgattatacc ggtacaagtt ttacctcgat
    11101 tggacacggt cgatttactc cggaagctgg tgcacaacca gcgattggga aagtagggaa
    11161 gacagaacaa gttcaagagg caaaagttga ggttattttg ccggaaacta tcgaaaaaca
    11221 agtgattcaa gccatgcggt cagctcatcc ttatgaggaa cctgcttatg atcttttcgc
    11281 tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta ggtgaattgc cgcaagaaat
    11341 ttctatagag gcatttgtag aacaagtaaa agaagcgttc caactagatg gactgagaat
    11401 agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt gcgatttgcg gtggaagcgg
    11461 tgaaaaattt tatccgcaag caatcgccca aagagcagat gtctatatca cgggagatat
    11521 ctattatcat acggctcatg atatgcaaag cgctgggttg attgcaattg atcccggtca
    11581 ttatattgaa tcgttatgca agcaacgatt tatcgagaaa tttgaatctt ggaaacaaga
    11641 agaaaattgg gatattaact ttttcgtttc tgaaacaaat acaaacccat ttcaatttaa
    11701 ttaaaaggag agaatactat gtacgaaaat cttttgccac gttttttacg atatgtcaaa
    11761 acagaaacac gctccgatgc aacaagcaaa acgactccat cgactcagac acaagttgct
    11821 tttgcgcaag aattgaaaaa agaattagaa gagttaggct tagctgatgt tttctataat
    11881 gaagataacg gattcgttat gggaacatta ccaagcaatg ttgatcatga agtgcgttcc
    11941 attggattta tttcccacat ggatacagca gattttaatg cagtaggagt caatccacaa
    12001 atcattgaaa actatgatgg cgagtctacg atcgtactag atgcggaaga acgctataca
    12061 ttgaatacaa aagattttcc aaatttgaaa aattatgaag gccacacatt gatcacaaca
    12121 gatggcagta cattactggg agcagatgac aaatcaggga ttgcggaaat catgactgca
    12181 atggagatct tgatcaataa tccttctatc cctcacggta ccatcaaagt ggcttttggt
    12241 cctgatgaag aaatcggtgt cggagcggat aaattcgatg ttgagcaatt cgatgtagat
    12301 tttgcttata cagtcgatgg tggtccttta ggtgagttgc agtatgaaac gtttagtgct
    12361 gctcaagcaa atattacgat ccaagggaaa aatgtacatc cagggacggc aaaagataca
    12421 atgatcaatg ctctacagtt agcaatcgat tttcataatc agcttccagc cgatgaagtt
    12481 ccagaaaaaa cggaaggata cgaaggtttc ttccatttga tggcattgga tgggaatcct
    12541 gaagaagctt ctatgagtta tatcattcgc gatcatgatc gtgaaaaatt cgaagcacgc
    12601 aaagcacaaa tcacacatat ccaagaagag ttgaatcagc gatttgatca agaacgaatc
    12661 aaagtagata tgtatgatca atattacaac atgaaagaaa tcattgaaaa agatatgagc
    12721 atcgtggaat tagcgaaaca agcaatgatc gagcttggta tctctccagt gatcgaacca
    12781 gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg gtattccgac gccaaatcta
    12841 tttgccggag gagaaaatat gcacggacga ttcgaatttg tttctttaca agtaatggaa
    12901 aaagcaacag acgtgattgt aaaaattgca gaattgaaca caaagagtta aaatcgaaaa
    12961 agaacagctt atgccaaggc gttcgtccca atgatggaat gtcatctgtc taaggctaaa
    13021 gggcaggaca atggaattgg ttgtcccgcc ctcttttttg aagagtaaat aggaggtaaa
    13081 gaatggaaaa gctgctagtt ttttataaca aaagttcagg gaaagatgag ggtgagaaat
    13141 tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc agaattatat gtagaactag
    13201 ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa gaaagcagaa gccatagaag
    13261 ctgatacttt agtaattata ggtggagacg gcacgattca tcatatcgtc caagcgttcc
    13321 aaaaaacatt atctcaatac catgtcggat tacttccagg tggaactgtc aacaaccttg
    13381 cacgtgttct tgagattccc ctcaaaaaag aagaagctgc tgacgtgatc ctggaaggca
    13441 atgtacggaa aatagactat ggtcaagtca atgatgaagt cattatctct acactgacaa
    13501 tcggaatctt agcagataca gcagtttgga tcagtcaaga agaaaaacag cgttacggct
    13561 cgtggatctt tatcaagaag ttttttaagt tattgctgaa gaagaaaaaa tatcatttgg
    13621 atttagaaac agacgaggag cattggaaag gaaaatccca actgttgaca gtcacgatgt
    13681 ctaactccgt gggcggtttt acaaattttg acgactcagc gaccctagat gatggaaaat
    13741 ttcatgtaac gatcgtacct tccttagata ttttccgatt tattttttat ttgcctagaa
    13801 tcatgagagg caaattctat acgatccccg gcattactta tttcacagcc aaacgcatga
    13861 aaatccaagc gcaggaaaaa tctgtgggca ctcgaacgga tggagacacg acagatgatc
    13921 ttccagttga attatctgtt gtatcaaaag gattaacagt ttattcacca aagaaagaag
    13981 aaaaagctta gaaatagtag ttaacagaaa gttggaagaa gagtaatgga tgtattgtta
    14041 cacaatcttt tgtggtttta ccttttcatt gtgaattttt acttattttg tctgatgggg
    14101 tatgataaac atcaggctag aactagcggc tggcgtgttc ctgagaataa tttgatattt
    14161 gtcagcctgt taggcggtgg tttgggagga ttgatctctc gaaaagtttt ccatcataaa
    14221 actcggaaaa agaagtttac tataggattc gctgcaggtg tacttgttga tttgattctc
    14281 atttattttt tccattaagg agcaggagat gtcgtctaaa ggaacgaaaa ctaaaatcat
    14341 tctgaatctg cttttgctga ttgtgatttt tgccattatt tattatttaa tcaatcaatc
    14401 atttgcagat atctttgccg aattgctgtc taccagtgtt gttgttttgg cagcggctgt
    14461 tatattaggg acagtttacc aaatcgctga agggcgctca atcaaagaaa ttgctgaacc
    14521 tttcgcgccg aattttacaa cgaaagatgg gttcttgacc tctagctata ttgcgtttta
    14581 ccgaatcgtt tcgtttggta caggaacgct gatctcggag atttattttt ataacaaaaa
    14641 aggaatgaag ctatcccagg gagcaggggt gacagcactc catatgatta tgtacaaact
    14701 ggcagtgatc acttatgcat tagctggctt ggtcatccaa ttctctcttt tttattctaa
    14761 tggtccatca atgatctggt ttatcatcgc tggtatcctt ttgacgatcg tgattgttgc
    14821 ctttttattg gcgatgtccg tcagcttgaa tgttcaaata ttttttgtca gcttatcgaa
    14881 taagttattt aaaagtgagc gtttgcggaa atgggtggat acatgcaatc agcagatcta
    14941 ctctttaagg gaaacggtcc aaaccattat ccaagacagg actgcgctat tgcggattta
    15001 tctatggaat atgttaaaac ttgtattttg gtatgtactt ccttatttga ttctgattga
    15061 aaaccatcct aaaatagatt ttctactgac catttcgttt atcagctttg cagtagtgct
    15121 ttcaggggtt attcctacgc ctgctggtat tggttccttt gaatttgtct atttgttatt
    15181 attcagacca gttgttggaa cagttgacgc agtttcttcg cttttactgt atcgtttcag
    15241 ttcctttatc ttgccatttc tctatggatt tgtgtatgtg attgctgaac ggcgaagagt
    15301 gatcaaagaa gaaataatag aagtaaaaaa agagaataaa gaaaatcaag ggtaggtctt
    15361 ttataagatc tacccttttt atcaaatcaa agaaaaacgt tatggataag ataattttac
    15421 gtttgtttgg ctcttttttt atatacatac aatcaatttt gtttttttaa gatttatata
    15481 agtcgagaaa aaaagtcaaa aaaccatgtg tatattttga ccaaaattga ccaaagcgat
    15541 ataatagttt ttgtaaagat caaagaaata agtgatctta cctttaatta ggagggtata
    15601 cctgtatgaa tattgagaaa atgactacga cgcttcaaga agcgattgct gaagctcaac
    15661 agattgcggt cactcgaaaa catcaagata tcgacattgc ccatgtatgg aaaatctttt
    15721 tacaacctaa tcattttggt cgaaatttct atacggatgc cggattggat gtcgaatctt
    15781 ttgaacatga aattgatcgt ctactggatg agtatccagt tgtttctggt ggaaacgtcc
    15841 aatatggaca aaacctcagc cagaatttct ttcgattatt gaatgaagca gaccaaatac
    15901 gtgaaagctt cggcgacgag tttctttcaa cggaagtcgt tattttagcg ttgatgaaat
    15961 taaaaaatta tccgttaact gtttatttga ataaaaacgg attatctgaa aaagaattgc
    16021 gtaaaaacat cgaagagatg agaggaggag aaagagtgac ctcacaaaac caagaagaac
    16081 aatacaaagc attagaaaaa tacggcgttg atttagtaca gcaagtcaaa agcggaaaaa
    16141 tggatccaat catcggtcgt gatgaagaaa tccgtgatgt catccgtatt ctttcacgaa
    16201 aaacaaaaaa taatcctgtt ctgataggag aacccggtgt agggaaaacg gcaatcgttg
    16261 aagggcttgc tcaacgtatc gtacgcaaag atgtgcctga aaatttaaaa gataaaacga
    16321 ttttttcatt agatatgggt gcattgatcg ctggagcaaa attccgtggt gaatttgaag
    16381 aaagactaaa agcagtcttg aaagaagtca aaaaaagtga tggacgtatc ctcttgttca
    16441 tcgatgaaat ccacaatatc gtaggggcag gaaaaacaga aggaagtatg gatgccggaa
    16501 atctgttgaa accaatgctt gctcgcggtg aattacattt gatcggtgcc acaactttag
    16561 atgagtaccg tcaatatatg gaaaaagaca aagcgttgga acgtcgcttt caaaaggtat
    16621 tagtcaaaga accaacagtg gaagatacga tcagtattct gcgtggatta aaagaacgtt
    16681 ttgagatcca ccatggtgtt aacatccatg acaacgcatt agtagcagca gctactttat
    16741 ctgaccgtta tattactgat cggttcttac cggacaaagc aatcgatttg atcgatgaag
    16801 ccagtgcaac gattcgggta gaaatgaact ctatgccaac ggaattagat caagttacta
    16861 gacgtttgat gcagttggaa atcgaagaag ctgcactgaa aaaagaatca gatgatgcaa
    16921 gtaaaaaacg cttgaaaaat ttgcaagaag aattagctga acttcgtgaa gaagcaaatg
    16981 cgatgaagat gcagtgggaa acagaaaaag aagaagtcaa ttctgtttca gcaaaacgtg
    17041 ctgaaatcga taaagcaaaa cacgaattag aagatgcaga aaataactat gatttagaac
    17101 gcgctgctgt attgcgtcat ggtacgatac ctcaattaga aaaagaattg aaggaattag
    17161 aagctaaagc aaaagacagc gagattaaga tggttcaaga gtccgttacc gaaaatgaga
    17221 ttgctcaagt cgttggcaga ttaacgggta tccccgtaac gaaacttgta gaaggtgaac
    17281 gagaaaaact aattaaattg aatgaaacac ttcataaacg agtgattggt caagatgaag
    17341 cagttgatgc ggtcagtgac gcggtaattc gttcacgggc tggattgcaa gacccaaatc
    17401 gtcctctagg ttctttcctt ttcctaggac caactggtgt cggaaaaacc gaattagcga
    17461 aagcgttagc tgagaacttg tttgactcag aagatcatat ggtacggatc gatatgagtg
    17521 aatatatgga aaaacatgct gtgtctcgtc tagtcggagc tcctccaggc tatgtaggtt
    17581 acgaagaagg tggtcaatta acggaagctg tcagacgaaa tccttataca atcgtcttgt
    17641 tggatgaaat cgaaaaagcg catcccgatg tcttcaatat cttgttacaa gtcctagatg
    17701 acggacgctt gactgattca aaagggcgag tagtagattt caaaaatacg gttctgatca
    17761 tgaccagcaa tatcggttca cagttactgc tggaaggcgt gactgctgat ggaacgattc
    17821 cagaagcagt ggctgaacaa gtcaacacgc tattacgtgg aaacttcaaa ccagaattct
    17881 tgaaccgaat cgacgacacg attttattta caccattgag cctagacaac gtgaaaggaa
    17941 tcgttgacaa aatggtggcg caattggcac aacgtctgga acatcaagaa atcctgctga
    18001 caatcagtga tgaagccaaa acgtggattg ctgaaaatgc ttatgaacca gcatatggtg
    18061 caagaccatt gaaacgattt atcactaagg aagtagaaac accactagct aaagaaatcg
    18121 ttgcaggaca tgttatgcct aaatcaaaag taacgattac tttacttgat ggacaacttc
    18181 atttcaaaac agaagagcta gaagaaatcg tctaatcttc cattgagact ataaaagaaa
    18241 aaaggggtct gacacaacta gtgtcagacc tccttttttc aaataaacag tgttccagaa
    18301 gtagtttctt cgaaaaataa gccgaaattt cttgaagctg cccgcttctg tcacaacctc
    18361 ttttattagg tatttttcac tttttctaat ttataacttt caaagtaacg ctcatctttt
    18421 ttactttgag accatgcaaa caatcccatt aaggcagcac ctatcgtatt tgtcagcaag
    18481 tcgcccatcg tatccatcaa tgctgctcgt ccgacaaaca gtgtgccatc agaagcagca
    18541 aaacgttgaa gattcatgcc taagaactgg tcgcataaga attcccagaa ctcccaaaac
    18601 acgccgcaaa gtccagcaaa cgcaaagccg aataagagga acaaccaagg actggttttt
    18661 gatatctcag catctttcat caagtaacca atcaatccat agccaagagc tgtcaggacc
    18721 attggactca ccgcatgtaa gattttgtcc caaaaagaga tgatactgat caaatgcatc
    18781 cctgtgccta aaaagacaga gatgaacaag aaaaaccagt aaaacaaaac gattgctgct
    18841 ggtattttaa gtttgaaaac tttcaaaatg acttgtggca tgaaaatcaa tgcaatccct
    18901 aataaagatt ccacgataaa tgtgattcct tgagaagtcg aatatttttg tgtgataaat
    18961 tcataaaaat tatagcagac aacgaataca ccaaaagcaa gtaaagctat ttgcaccttt
    19021 ttgatttctt taatcactga aagtccctcc atctaaaaat ctttttactt ctgaaagcaa
    19081 ctcaatttct gaagaaacca cttttccgtt caatttgatc aaacctattg tatataagtt
    19141 caggtagtga aattgatttt cagctgtttc ttgtagtgca gcgatttttt tcgggtgggt
    19201 tccgccttgc tgtcgagtat cagtatataa gccgatcact gggatgcctt tcgcataagc
    19261 cacaccgatt tccgatgcta ccccggcatc gatcgtcagc ccatctagta aagcaatcat
    19321 taaatcacta gccaacactt tttctgtgtc agcaagagca atcattcgac tgtctgcata
    19381 agcagttttg tcattgattg cttcattttc ttgaggaaga tagatcgaaa gctcagaaga
    19441 gagctctctg atttctttaa ctaactgttg gttatataac aaatcacttt tagcaaacat
    19501 tggtgcggca aaataaattt tcatctaaac atcatccttt ttatcaaaaa taatcgctaa
    19561 agtaagcttc catgtttgga atgatacgct ctagtgactt cagtgtttct tcatctgttt
    19621 ctaaccgttc gattcttgcc aaataagaag cacggcgata attcatcatc aagcaagtca
    19681 atgtttggat atccagtcga acggattttc ctataggttc atttaagaca cggacttgtc
    19741 cctgttcatc ccatgttaaa gcgaagacac cattattcca aggagcgaca ggatcttcaa
    19801 tgatgaaatg aaaagcatca gctgttccaa caaacggaaa ccgttgaagg aattctttta
    19861 catcaacgat ccgagccata aaatatggtt cgatctgctc tttgatctga ctatcttcaa
    19921 tcaaaaatgc taaaggctca tttttataaa tatctccatg tacccagtag atcatggaaa
    19981 aatgtgcgga aataaagttc cataagccat ttcgagcttc ttgatcaaga tagaacattt
    20041 cttttacgtg aaatatttct tcagctaccc agtagaacag aacacctctt ggttcatggt
    20101 tgctatcgta gtagacagcg gctgtgcgct catcttcatt ttcgaaacgc caatactctt
    20161 cccaatgaaa atcactgcgg aataaagcac catggttttg ttgggcaaat ttgtcataaa
    20221 cggaaaatac gtctggatga tcaactggta atcgctcaac cataccactt actgggattt
    20281 gtttaggtag ctgtgtatct ctgattttaa aggaaagttt atcagacatg atttcccatc
    20341 ctttacgccg atagtagggg atactgtaag gatacaaata agagatccat tgcttattct
    20401 tgcgcatatt atccaatgca acgataatta gatcttgcat caatccatgg ttagcatatt
    20461 ctggataagt tccaactcca gtcacaccac ccattttata acgtgcaccg tgaatattga
    20521 cttcacaagg atagatagcg atttgagaga tcagtttatt ttcgtggaac catccgaaca
    20581 ctttcgatac ttccaaaatc ggttttttgg aacggataaa agctcgttta ttctcaaacc
    20641 cgctttcctc gatgtctgct tcagtcactt ggaacacata ggaaagcaat tcattgaatt
    20701 gatcgatatg ttcttgagtg accggtttta attcaagatt ttctctaaaa tctttttgat
    20761 ccattttttc atctccattt attagcttga taacagtata gcaaaattct acaaaaaaaa
    20821 cttctatagc tgactaatta aaaactcttt aaattactgc ttttcgttgg tataatggaa
    20881 agtgctgatt atatgaaaag aggagacgct atggatataa agaaaatgaa agaacgccaa
    20941 gagcagattc gtaatttttc gatcatcgct catatcgacc atgggaaatc tacattggcc
    21001 gatcgtattt tagaaatgac gcacactgtg acgtctcgcg aaatgcaaga tcagctgtta
    21061 gactcgatgg atttggaacg tgaacgtgga atcacgatta aattaaatgc tgtagaactc
    21121 aattacacag cgaaaaatgg agaaacatat atctttcact tgattgacac cccagggcac
    21181 gtcgacttca cctatgaagt ctctcgaagc cttgctgcct gtgagggtgc cgttttagtg
    21241 gtcgatgcag cacaaggaat cgaagcacaa acattggcca atgtttattt agcgttagac
    21301 aatgatttgg agatcctgcc agtcatcaat aaaatcgacc tacccgcagc agatcccgaa
    21361 cgtgtccgtc aagaaatcga agacgtaatc ggaatcgatg ctagtgaagc tgtattagcc
    21421 agtgccaaat cagggattgg gattgaagat attctcgagc agatcgtgga atatgttcct
    21481 gcaccaagtg gagacttaga tgcaccgcta aaagctttga tctttgactc tatttatgat
    21541 agttaccgcg gtgttgttct aaatgtccgg atcacggacg gtatggtcaa acccggagac
    21601 aaaatcaaac tgatgagtaa tggcaaaaca tttgatgtta cagaggtcgg ggtcttctcg
    21661 ccaaaagcag ttgctagaga ctttttgatg gtcggtgatg tcggttatat cacagcaagt
    21721 atcaaaactg tccaagatac acgagtaggg gatacagtaa cgttagcaga caatcctgcc
    21781 gctgaagctt taccaggtta ccgaaaaatg aatccgatgg tttattgtgg tctttatcca
    21841 attgatactt ctcgttacaa cgatttgcgg gaagctttag aaaaactgca attgaatgat
    21901 gctgctttac aattcgaacc agaaacctct caagcattag gttttgggtt ccgctgtgga
    21961 ttcctcggac ttcttcatat ggatgtcgtc caagaacgct tggaacggga attcaatcta
    22021 gaattgatca cgactgcacc atccgttatt taccatgtta ataaaacgga tggtacgact
    22081 gctacagtag ataacccggc agattttcct gaacctgtaa cgattcagga tgtagaagaa
    22141 ccattcgtca aagcacagat catggtccca aatgattttg tcggtgcagt aatggaactt
    22201 tctcaacgaa aacggggaga attcattacg atggattatt tagatgatta ccgagtaaat
    22261 gtcgtctata atattccgct ttctgaaatt gtctttgact ttttcgacaa attgaaatca
    22321 agcacgaaag gctatgcctc attggattat gaaatgtctg gttatcaaaa aagcaagtta
    22381 gtgaagatgg acatcttgct gaatggagaa aaagtcgatg cattaagctt tatcgttcac
    22441 cgagactttg cctatgaacg tggaaaagca atcgttgaaa aactgaaaaa actaatccca
    22501 cgccaacaat ttgaggtgcc aatccaagca gctattggac aaaaaatcgt tgcccgttca
    22561 gacatcaaag ccttgcgtaa aaacgtcttg gctaaatgtt atggtggaga tgtttctcgt
    22621 aaacgtaaac tcttagagaa acaaaaagaa gggaagaagc ggatgaaaca aatcggctcc
    22681 gtcgaagttc cgcaagaagc cttcatggcc gttctgaaaa tggatgagca agatagtgtg
    22741 aaataactta taatcaatcg aaaaacctat taaatcaacc tttttgagta gatgcaacac
    22801 tgaggattat ctgtataaaa gattttaaac tgtcatttgg ttatcacggc gatactaaaa
    22861 ttaacaaaat tggaatattt tttcttgaag atttttagct gtcagtatat gatgaaagca
    22921 tctcatagga ggtgcttttt tcatggttga taattcaagt ggaagggttc ttaaatctct
    22981 aagaaaagag aaaaaactat ctcaaaaaaa gttagctgat ttagcaggta tttcacaaag
    23041 tactttagtt aaatatgaaa aagggtcaag aaaaatccct aaagacgtag ataatactct
    23101 aagcaagatt ttgaatatag aaactctaat aaaagatgag gaagataaga ttgagattct
    23161 catcggtcaa ctaattgcat acagagatat gaataagtta cttaacaaag agttagctga
    23221 taatatagga attagtgaag tattactcag ttatgtgtta aatagaaaga gaaatccgtc
    23281 aaaagaaatg caaaaaaaaa tcgatatttt tctattaagt aatgaaaaag aaatttcgaa
    23341 ggagagtaat cgtgatagtg agatatttag tttatcaaaa gacgataaaa ttgttatggg
    23401 aaaaagaata cgagaagtcc gaaagaaccg ggaagagacg cttgaaaaat ttggtaaaaa
    23461 ctttaccatc tatacaggca aaaatgttat aagtagatgg gaaaaaggta ttaatattcc
    23521 agatattgaa aaactaatga atattgccta tcttggtaag gtcactgttc cttaccttat
    23581 gtacggagaa gattataaga atatacttcc aaaagatgaa agagttagcg attttaacaa
    23641 gataaattct tttagtatgg gattaaggat gaggaaaatt aggaaagatt attatctaga
    23701 aagggaggag tttggaaagc ttttttctcc ttcaatttca aaatggtcca tagataggta
    23761 tgagaatggt agagatatac ccaatactaa tcgaattatc caatatgctt atataggaaa
    23821 tctttcttta gaatttttga tttacgggat ataaaaatag tttacgtcga tacttattaa
    23881 gttatcgacg tttttttatt atagaaaaat ttataaaagg aggtttcttg atatgataag
    23941 aaacaaaaag ggtttgttga aagttgcttt actggcagct tttttctaag taactttttg
    24001 acaagctcaa tggcaacaca agcggaagaa gtaatacaag aaataactgt tatgaatacc
    24061 agctagataa aatgggctag gtatgttatc caacaattta gtaatcacta gaagcctagc
    24121 gaaagttcgc ttgataagtt atagatgagt attgatttgt agtctttcca aatcaaaagg
    24181 gtacagagcc acatgaaata tgttgtcctc tgcagcgtca ggatgggaat ccataccatg
    24241 gaaattcgga gatctgtgag ggaatactta cgtggttaat atgtcggttg tgttggacgt
    24301 agtaatggtc atagtgggta gattgactga attcttatat gagaaaatga aaccaacaag
    24361 aagatagggt tatttataca gaattgggga tcttttctag aaaagtaatc ggacaatgcg
    24421 agattcgtac ggtctttttt aaatttctct taaaaaaacg gcatcgaatg ggttgggtga
    24481 ttgataagca atgaatcata gactgatata gaataaaaaa atgattctat ttatcttttt
    24541 acatctatcg ttttttcaag taaccgcaaa ttgtatcagc attcaacaat ttttgtatac
    24601 aagttctagt aaaaggatct gaagaaaaag ttggttgttg tgaagataaa aaaagaatca
    24661 ggttttactg agtttttctt caaaatttgt gttattaggt taggacaaag ataagaagaa
    24721 gtatgaagat ttaaaaaagg aatgaggaaa agatagtaat ttccgattat tggagttttt
    24781 ttattttata aagaagaagc gaacaagttg tgattcctag atttcgcgcg tgattaatta
    24841 atgagcaaaa aatgcggaaa gttttagtgt ttaatacacg aggacaaagt ttcttaggta
    24901 ttgaaggcac acgtgtaacg gttgatgggt ggtgtgattg tttcgaggtt ggaaaacatg
    24961 ttgaacttat ggcatttact gagctaaaag ataagaacaa cgtggaattt gtcgagagtg
    25021 atattggttg ggacgatcat gaagaagttt atggaaaatt tattttgcgg aagggaaatt
    25081 cttatacgaa tggggaagca taagtagcga tttgttagag atcattgata ggattgaaat
    25141 tgtcgggaat atttatgaga atctagagtt atttgaggtg atagaatgag tgatggtttt
    25201 gaacaagtat caataattaa aaatcaagtt cgggagattc ttagaaaaaa aggctatttg
    25261 gtagatagtt attttgaagg agattacgaa acatgggtag gtgtctatgc tcgtccagag
    25321 aataaaccta cctatttaga tcctacgacg tcagaagatg cctatttaca aaataggtat
    25381 cgagtagatg gttttaagca agactttgcg gagtggtttg aatgggaaat tgaaaacgga
    25441 gaagtaaaag aagaataagc gtacttgttg tatcaaaata agtcagtaga acaaccaaac
    25501 cagtagctag taatgacaat aatactgggg aatcggatca ttatggtgtg attgatattt
    25561 ctattccaga agatcttaag ccagatcctg caacaattgg caacagtggc atgatttttg
    25621 attcgttaga tgaagctatg tcatatggtt ggaaagaaat taaggatgaa ggtagtaatt
    25681 ggtatggcat gatatttggt gatggtgaag ttgccaataa agctggtatt ccaattggga
    25741 aatggacagt tgatttccac gaagcaggtt ctttttttgt tctgtaatta tagtgggagg
    25801 gtaaaatcat tgatgaaatg tgttcagata agagcgccag ataaaaatag aagagacagt
    25861 tcacgtcgtt atcacttttg gtaacggtgt tttgttttat tatataaaat tctcaggaaa
    25921 aggaggaatt cttgtgtatg caaaaacgaa aatgaattgg ttgaaagcac tcgcattggt
    25981 ggccttatct caattctgtt tcttggtggg aactgctcaa gcacatgcag aagaaacgac
    26041 ggctacacaa cctacggtgg aagccattgc ccaagtcgct tctgaagtga cagaagtcca
    26101 accagcagaa gccggggtcc cagctggtga agccactgtt gaacaggccc ctgtgacaga
    26161 agcgccaacg gttaattcca aaacgactct cgatcagacg attggggaaa cacaacaccc
    26221 agggaatagt cctgttactt cacaaccagt tgaagaaact gcctcaatta caacccctgc
    26281 ttcagaaacg tccactccag aaggagaaaa agctgccgaa gcaacgcctt ctgtgaagcc
    26341 agagtcttct gagaaacaag cggaagaaaa gcaacgaata gaagaaacag tggaacaacc
    26401 agcggctact gctgacaaag aaggaaaaaa tgaggaaaac gttgaaaaaa caatctcttc
    26461 aaccgcagct agtcaaaatc aaccaacgaa actaaacaaa gaatctacgg ctagtattga
    26521 agatcaaaag aaaaaagagc aggacattcc aaaaacaggt acacctacac tgttagtaac
    26581 agtaccaacc aatcgttcgg ttcgtgttcg ccgtgcaacc aatacagctg ttttaggtgg
    26641 tcggaaaatt gattggggcg aaaatattgg tgttacttct agtccgaaca atccaaactt
    26701 cactttaaat tatatggttc gttttgagat taatggacaa gtggcgtttt gtattgagcc
    26761 tggtgtctca gccgcagaag gtcaagttta tacagctact gctttggaaa actatttgaa
    26821 aaattcaaat gtacggaaaa aaacaagctt gatctcttat ttaggatatg tcacaaataa
    26881 agacaaatcc gatgaacaat acattgcggc acaatttatg atttgggaag ctttgggcac
    26941 aaaaatccca atcaaaaata tccgtgttga ttatgatcgt cgaaaagcag aaattcaacg
    27001 tctgcttaat aattttgatg taagagctac ttttcataat caaacacata cgataaaaat
    27061 tggcgaaacg ttaaaagtaa aaagtggcaa tgactttcta caacgtgtaa aaggcattca
    27121 aaagccaaaa gggatcaatg tatctattca tgggaatgaa gcatggatca cagtcacaaa
    27181 agaagcacca gaaaaggcaa aaattcattt caatcagatt gaagtattgg gtacgccatt
    27241 agcgtatcgt cgtccaggtg cccaaacaat tggtatattg aatccttttg aacctggtga
    27301 ctctgttgtc aatttgacta ttttgaaaca gggaaacatt cgtgtcttta aagaagatgc
    27361 agaaaccggt gtgaaagcac aaggcgcagc ttctttagaa aatgcggtgt atggcttgta
    27421 tcaagcagat gggaaaaaga tcaaagaaat taccttgaaa aatatgaacg gcaaagtaca
    27481 ggcagaaatt aaagatcttg atcctggcaa atatgtttta aaagaaatca aagcgccgaa
    27541 aggctatgca ctttctgatg aaacaattga aattgttgtt gaaccaggaa aaactgtcaa
    27601 tgcgaccgca aaagatcaag tgatcaaagg aaccattgat ttagtgaaaa ttgctaataa
    27661 agatttagtc gattcaacca atccagataa caaaccaaaa ttagctggca ttgaaatctc
    27721 tttgacgtca aaaacaactg gcaaagtggt gaaaaccgtt gtgacagaca atgatggtta
    27781 cgctactttt ggtaaaaata ctgttgtctt tgatacctac attctttctg aaaccaaagg
    27841 aaaagaaggg tataaactat ttgaaccact tgaagtcaca atttctgaaa atgggcaaac
    27901 tttccattat gttttagaag ataaagtaat tgaacaacgt cttaaagtgg tgaaagtgga
    27961 tcaagaaaca ggaaaagtga ttccacttgc caatacagaa ttcaagattt ttgatacttt
    28021 agccaatcgt tatgtcacaa tggagattcc aaatgataca gaaacaacgg atatctttaa
    28081 aaccaatgaa aaaggctttt tcctaactaa cggtacactt acttatggaa aagagcgcta
    28141 tcgtttggaa gaaatcaaag cacccgtaaa ttacgttttg aatcaaaacc cattagtgtt
    28201 ctcgattacc aatgatacag aaccgatcaa agtgatccat tttgcgaacc gtcaagcacg
    28261 gaaaagtgta aaattgttca agtatgaaga atggaacaag aagaaaacac cacttgccgg
    28321 tgtgacgttt acgctatata acggaaaagg tcaagcaatt ggtaaatata ccaccgatca
    28381 aaatggtgag attttagtaa aaggtctggt tgccaataac tattacttta ccgaaaatgt
    28441 accattagaa agtttccaac caaatacagg caaacaagca ttcactatta cattcagaca
    28501 aaatgcgcca gatgatgggg aaacgttatt ggtttctgta cgtaactatt tgattccacc
    28561 aaaattagaa acacaagcgt caaataaagc cgatggtgaa aaagtggttg atccattaga
    28621 aactatcgtc attaaagata gtgtttctta taccaacctg tttgtcggca aagaatacac
    28681 tatttcaggt gtattgatga acaaggcaac gaatgcgcca attttacaag aaggcaaaga
    28741 aatcacaagt atcttgacct ttattgcaga caaagccaat ggcacaaaag atcttgaatt
    28801 tgtggttaat gctagtgttc tgaaaggtca aacaatcgtt gtctttgaaa aacttttccg
    28861 tgatggcatt gaagttgcgg tacatgtgga tatcaatgat gacaatcaaa gtgttcgggt
    28921 aaacaatcca acggttcata ccacagctgt atttgaagac gggttgacga tcgcagaccc
    28981 aacggggctg ttcaccttaa atgataccgt tgaaattaat gagatccttc caggaaaagc
    29041 ttacttggtc aaaggaatct taatggataa agcaactggt gaagccttac ttgttcatgg
    29101 tcaaccggta gaaagtgctg ttggctttat tgccaaagca gaaaatgaaa cggtgatcgt
    29161 accatttacc tttgatttga caggtttaaa tggtcgtgaa atggtcgtgt ttgaaagtct
    29221 ttttagaaat gacgaagaac tagcaaacca taaagacatc aacgacaaag gtcaaacggt
    29281 acgagtaacg aacccaagtg tacgtacaac ggctacacat aaacccactg gcttgaaaga
    29341 ggttaatcca ttaacgaaag tcaccattac agacgctgtg aaataccacg atttgatcgt
    29401 tggaaaaaca tacacagtca acggtgttct aatggacaaa gtaacggaaa aaccagtgct
    29461 agtagatgga aaagaagtct caagtacctt aacgtttgtg gcagaaacaa ctgatggtac
    29521 agtcaatcta gactttatcc tagacgcaca tgcgttgcga agcaaagaag tcgtagtttt
    29581 tgaagatctg tttagagaag gcgtattatt ggcttctcac gcagatatta ccgacaaaga
    29641 ccaaaccatc aaaatcgtca gtccaaccat tacgacaaaa gctaccaatg caaatggtgg
    29701 aaaagagatc ttagctttac caaatcaaaa ggttgtcgaa tgggttcaag ttaatgggtt
    29761 agcaatcggt caaatttaca aactgattgt tcaaggctac ttaacaccag acggcacccc
    29821 atttgaagga acacatgcgg aaaaagtctt tatggctgat aaagaaatga tggaattctt
    29881 gtttgaattc atcgttgacg gtcgcaacct tgcaggaaaa ggattgagtt ttgcggaatg
    29941 gctccaagcc aaaacagaag atgaaaaaga aacgtttgaa gaagtcgcaa aacataacgt
    30001 cgatctaaca aacaaagatc aaacggttca atttgtggag gcaccaaaac caccgcaacc
    30061 agtaaaacca aacgaaccac aacctgtaaa agaagtgaaa caagctgcaa tggtacctat
    30121 gttgccaaaa acaggctctg aagacaatgt attatggatc atgattggta tgatctttgt
    30181 ccttgcggca ggattgattg gttatgattt gtataaagtc agagactaaa gaggacgaac
    30241 aagcggttag gcaactagcc gcttgtttag tttttagaaa gaatttttta tcaaaggcaa
    30301 ataaattatt gtgttgagta gtaaatgatc atgaggatac accaagaaat actgagtgta
    30361 ataaataaga atttaaggat ccaaaagaga tagaatataa ttatgtgata atacagtttc
    30421 tttttttcta tagaagaaat cacgtttagc tactcctaat tgctttctta ctttatttag
    30481 ttctgctagg tagcagtatg agaattcaaa atacattctt tgtactttga agaggatttt
    30541 tttcgaatgt ttatttgtgt taattatttt ttgtatttct ttggcaaaat aaataagttc
    30601 ataaggcaaa taaaagtcta tatttttatt ttcaactgtt tggattagat gttgaattgt
    30661 ttgttttcgt tctagaatat tttttcttgt aattttttga aatagataat tttcaattga
    30721 agatttaaat ggaataaaca tttcttttgt tagggtttca gtgcttttct tttttcttgt
    30781 atagcgatgt tttaaataaa agctagaaat ggaccaaaca atagtgacga ttgaaactat
    30841 acttgagatg ataatgtgta attcatttga aaatagttgt tgaataaaag tgtttataaa
    30901 aataatctcc ttttttataa aaagttagaa aggatgtaaa agagttggaa gaaatattct
    30961 tttgtatgat ggtagagttt ttgtttgtat caattggttt cttaattatg ggggaggcaa
    31021 ttatgatata tctttttccg tttgacagca aggatgagtg catctttagt agtattatta
    31081 ttttctttat tttagcatgt gtgtggattc ttgctaggga aatatatcat cagtttgtgt
    31141 tggaagatgg aaaaggaatt attacttggt ttttacagaa aagaaaataa tggaggaaaa
    31201 aagaatatgg aaaagaaaaa tataaatatt tgttttttga gtggaaaaga atgtacgtat
    31261 gaggagtgta ctgatatttc tgtgcatgaa cgagacggga tgttaatagt agatatgtct
    31321 cctagtgatg agccatttgt tgttaaacat tactttccat tgtgtaatat agagtcgttt
    31381 agaattgatt gtgaatttta agaggaggaa aaatgatatg gcagaaaaag cattgcgtat
    31441 gaagaaagag tttttaggtc aggtattttt attgggttca cgggaaccta atccaccaaa
    31501 ggaaattaat ggtgtgtctg ggcgatatgt tcaattacaa gcagaatgtt cgcgagaaga
    31561 acagcctgct ttttttgttt atctcacagc tgaagaggat aaaagattac gattaccaga
    31621 aaaagaggta cgtatttcat ttgaagaagc ttattttgtt tttcgtaaag aaagagtgaa
    31681 tagtcgtgaa acaaatgata tgggacaaac agagcgtcga acaactggct atcaagatgt
    31741 cggtgtcttt ttaactggtg taaaaattga ggaggttttg taagatggga ttaaaatttc
    31801 caaataatta tgatattaca gaagcattag atgaaacttg tataggagcc gtacgctttg
    31861 gcgcattaga agagtccgat cgtgtatttg ataatgaaac aggcgattat acgggagaaa
    31921 ttcgatcaca gcgagcacaa ttagtttttg gaaatccaga tgaaccaaag attgcacgac
    31981 gagttaaatt tccagcagat gtagatttga ctggttttaa acgacgtgat cctgtgaaat
    32041 taattgatcc agaatatcat gtggtatata ttccagcaca agatggcaat cgtgaatatt
    32101 cagatttagt gatttatgcg aaaggattag agaaagtaga agctccaaca aataattcta
    32161 atccgaaaca atcacaagaa aaaggtttag atcaaaaaca accaagtgct gattctaagg
    32221 aaaaagagaa aaaataagtt ttttaaagag taggaggaag tcttcctact ctttctattt
    32281 aaggtagaag gaaggggaga ttttgaatct ggacaaaacc aatcaaacac ttcgagagat
    32341 tatggtttgt tgtcaaaata tccaacaatt gattgagtat tttggagaca atccaacgat
    32401 ttttatacaa acaaatgagt atatttgtgc ggttattggg cagtgtatgt ttttacgtga
    32461 tcttgttcgt ttttcattac cgtatcagtg gaaacaagac catcaggaag tggtttggat
    32521 tcgtgtagta cgaatatgta atatgtgtgc acacccatat aaaaatcgat taaatctttt
    32581 tcgattatgg ttttttatta aatgtgatat tccagtatta gttgcttttt gtcaaaaatt
    32641 tttagcacag gagaagtaaa aacagaaagg aggaatgtga caattggaac ctcatttttt
    32701 taagtatcgt ggtcgacgaa ttcgttattt caatcgttat ttatggtttt gttattatgg
    32761 tttctttctg ttaccatttt tgattctatt aagttggttt agttggaaag tattttatcc
    32821 tcgtttaaat acgtattcga attggcaaat gtatcttatt cctggaatca gtattgtgat
    32881 gtatttgttg ctggttagtg gattaatttt aggtttgatg cgttggtcgc gactaaagga
    32941 aggatatttt gcttctgttt attggcgtca gttacttgct cgaatgttaa ttgacaatcg
    33001 gtttgtttat acaaagaagc aagttggtga gaaacaaacg aaggaaaaaa tgagattacc
    33061 aaaagtttat ttttatcaaa agaaacaaga attagtggtt tcttttcctt tagatggtgg
    33121 ccgtttccac gaacgatttt taaaaatggg tagcttatta tccgaagtat ttttgcgtga
    33181 tttaattcgt gaagaacgag aaaaagcccg aatcaattat gtatttcttt ccgattctgg
    33241 tcggattcct attgatcaat gtatagcaga aaatggacgt attcatttaa tgaaaacatt
    33301 ggattgggta tatgatcaat cgccaaatct attaatttct ggtgcgattg gtggcggaaa
    33361 aacctattta ttgtattcat tgatcaaagc ttgtttagat gtagggacag tagatatttg
    33421 cgatgggaaa gcggcagatt tagcggcatt aggtgatatt gatgtattca aaggacatgt
    33481 gttttacaaa acgaatgaag aaatgattat ttgcttacga aatgcgttac aagaaatgaa
    33541 tcagcgatat acctatatga aaacgatgaa agagcctaag tatgaaccag ggaaaaatta
    33601 tgcttattat gggataccgc cgcattttat cttttttgat gaatgggcag ctttctatgg
    33661 ttcgttggat atggaaacga gaaacaaagt tgataaatta attcagccat tagtattaaa
    33721 aggtcgtcaa gcaggcatgt atgttatttt agcgatgcag cgaccagatg cggaatattt
    33781 tccaagtggt gttcgagata gtttaacgtt ccgtatttct gttggccgtt tatctcccat
    33841 tggctattta atgacttttg gagaggattt taagaataag ccgttttata acaaagatca
    33901 acctggtcgt gggtatgctt ttcctggttt tgatgtgcct gtagaattct ttgcgccatt
    33961 tgtttacaaa gactatcact ttattgagga atttaaaaaa tttgacacaa tgattgtgca
    34021 agatttttct gtactgaaag aagaggcgtt tactagttac gaagaacaaa ttgatcgtca
    34081 agcgcaatcg ttaaaaggaa atacgaaaac aaatggggta gatgaggaaa gttatacact
    34141 tttataattt agaaggaagt ggtgaagtat gggtgaagtt ttgtatcgtg tttcttcagc
    34201 tgcgggtgaa atttcacctg attttgcggt tcgaagatta tacgagtgga ttaacaaagt
    34261 agaatactac acaaaaggtt cttatgtttt ccgacgaatt gagcgcgaga cattgtttgt
    34321 tacgagaaat cagattgttt tgacaaaaga ggatatattg cgttttcgtc aggtatatcg
    34381 gttatgcaaa gagaaaaaca tacagttacg tttagcgatt ttacagtgtt ttgctcctga
    34441 acagtataaa gaattacaag aaaaagaaga ttcattggtt tagatatcgg ggtggctttc
    34501 atagggacta tgggagaaca cattgtccga gtatgtgctg ccgcggcttg tgtcggcgaa
    34561 gccaccgaga ccagtcgtgg cagcacatgc ttggcatgtg tgcccacaaa gaggtcgtgc
    34621 gcttgcgcac ggcctctttg tgtaaagccc cccgatatat aatatggggg gcagaaataa
    34681 cataacacaa ataaaaaagt gtatttaaat cagttatatc aaggctttag ctaaggttag
    34741 aaggagtact cctttttaga aaaattaggg ggtaccacct gttatatcgg gatttctagc
    34801 agattaagaa aatcgaaatt tgttatttta taaaggaggt agaatatgac acctattgaa
    34861 ttgcgaaatt atcgaaaaaa attgagtgct attcacgctt caggtggagg gaaaaaatta
    34921 ctctctcaaa aacgttttgc acagttattg ggatgctcgg tagattatat aaagaagatg
    34981 gaacaaggaa aaaagaaaat cccagaggat ttcgaaaaac gaatcaagga acgctttcga
    35041 ttgtatatga aaagcggaat agatcttcga gcaatgatcg actatttgcg tctctccttt
    35101 tttgggacag actacaaaat agtgattcaa cgtgtgttac gaatggatct aacagaattt
    35161 gaaacacgtc cgacgggctt gtatcttttt gattgtcttt ctgttcgtgg aaatatctgg
    35221 gtattctggc attcaaaaca aactgtaaca caaaatgtac ttgtacagtt ttcgggtcaa
    35281 ggttgccgag aatatgagga agtcatgcgt gaacaacgaa cagattggcg aacatggcta
    35341 tattccattt ggaaaaatca aggaaagttg gaagctggtt ataaacgagt acaatgtagt
    35401 cgattcgata ttgcgttaga tgaactttgg ggattggaaa cagaagaaca ttttgatctc
    35461 tttgaattac tagaaaaaca aaaagcaggt ttattagaaa tggactttaa gaaatttcag
    35521 aatattggcg gttgtttttt agatgatggt tatttccgaa gcgaagggtt atccttgtat
    35581 tttggatcgc gaaagagtcc attgtttttc aatttctacg aaaaacgttt tgaaattgcg
    35641 aatcgtgaga aaatttcaga aatagaagca ttaacgaaat atggcattta taatcgatat
    35701 gagttacgcc tagctaatga aaaagcaaca caagcagtag aagcgtttat tttggatcat
    35761 tcccctaaac gtttaggaga gattggcgtt ggtttaatta atgcaaaact tattgtttgg
    35821 gatcgactgg aaagtgatgc acgaattgtt gatgaacgat gggcaaaatt gtttcgttcg
    35881 atggaagaat tggattttac cgtaaagcca gaaaaattct cattagatcg ttcagaacgc
    35941 tggtttttga aacaagtagc tccgacatta aagttattac aagaagcaga tcgtatttta
    36001 ggaagagact ttgtacaaga aggaattgag aatgctgaac taaatgacga gcaacaaagc
    36061 tatctagaat ggatgggaac gtcaggaggg aaaattgcat gaatgcatat acgacaacag
    36121 aatggctgcg tcttttggat ttaaaggctg caattgaagc attaaatgaa aagatggttg
    36181 atttgtctta ttttcgtttt cgagttcctt atattgaaca agcggtaaag gctggacgtt
    36241 atcaggaaaa agaaaactgg caagaaattg cacgcttatt agaagttcgt aaggggtacg
    36301 aacaagaact cgaagagttg gaatttagcc gaagaaaagg aacgctaaaa ttcattcgct
    36361 tttatcgatt ttcattgccg gtacctgcga ttttagcggt aaaaaaaggc tgcgataaaa
    36421 tgaagatata cgaaaactgt gtggcagcac ttagcaacga aaaacctctt gtggaagaaa
    36481 tctcgttggc aactgttact tgggaaatga atcgccagcc aacagaagaa cagacgtatc
    36541 tttctttaga agagattgag ctggaattag aggaaatcgg ccgctatgcc acttgttcta
    36601 cctattgtgg atcggtgatt agtattgctg gtgtgattgt ctaaatgaaa aaa
//
LOCUS       DACEPW010000007        35843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEPW010000007 DACEPW010000000
VERSION     DACEPW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.007"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..3288)
                     /gene="dnaE"
                     /locus_tag="IW819_000272"
     CDS             complement(<1..3288)
                     /gene="dnaE"
                     /locus_tag="IW819_000272"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1243406.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLL"
     gene            3519..3704
                     /locus_tag="IW819_000273"
     CDS             3519..3704
                     /locus_tag="IW819_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1243407.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(3766..4962)
                     /locus_tag="IW819_000274"
     CDS             complement(3766..4962)
                     /locus_tag="IW819_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1243408.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(5132..6568)
                     /locus_tag="IW819_000275"
     CDS             complement(5132..6568)
                     /locus_tag="IW819_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1243409.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            6861..7670
                     /locus_tag="IW819_000276"
     CDS             6861..7670
                     /locus_tag="IW819_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1243410.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(7856..10009)
                     /locus_tag="IW819_000277"
     CDS             complement(7856..10009)
                     /locus_tag="IW819_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1243411.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(10303..11151)
                     /locus_tag="IW819_000278"
     CDS             complement(10303..11151)
                     /locus_tag="IW819_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1243412.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(11375..13315)
                     /gene="thrS"
                     /locus_tag="IW819_000279"
     CDS             complement(11375..13315)
                     /gene="thrS"
                     /locus_tag="IW819_000279"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1243413.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(13652..13831)
                     /locus_tag="IW819_000280"
     CDS             complement(13652..13831)
                     /locus_tag="IW819_000280"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1243414.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(13878..15701)
                     /gene="cadA"
                     /locus_tag="IW819_000281"
     CDS             complement(13878..15701)
                     /gene="cadA"
                     /locus_tag="IW819_000281"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1243415.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            15979..18258
                     /locus_tag="IW819_000282"
     CDS             15979..18258
                     /locus_tag="IW819_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1243416.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(18351..18998)
                     /locus_tag="IW819_000283"
     CDS             complement(18351..18998)
                     /locus_tag="IW819_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243417.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(19177..19896)
                     /locus_tag="IW819_000284"
     CDS             complement(19177..19896)
                     /locus_tag="IW819_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1243418.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(20027..21178)
                     /locus_tag="IW819_000285"
     CDS             complement(20027..21178)
                     /locus_tag="IW819_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1243419.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(21395..22135)
                     /locus_tag="IW819_000286"
     CDS             complement(21395..22135)
                     /locus_tag="IW819_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1243420.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(22139..22462)
                     /gene="rsfS"
                     /locus_tag="IW819_000287"
     CDS             complement(22139..22462)
                     /gene="rsfS"
                     /locus_tag="IW819_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1243421.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(22519..23112)
                     /locus_tag="IW819_000288"
     CDS             complement(22519..23112)
                     /locus_tag="IW819_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1243422.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(23096..23743)
                     /locus_tag="IW819_000289"
     CDS             complement(23096..23743)
                     /locus_tag="IW819_000289"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1243423.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(23756..24067)
                     /gene="yhbY"
                     /locus_tag="IW819_000290"
     CDS             complement(23756..24067)
                     /gene="yhbY"
                     /locus_tag="IW819_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1243424.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(24083..25192)
                     /gene="yqeH"
                     /locus_tag="IW819_000291"
     CDS             complement(24083..25192)
                     /gene="yqeH"
                     /locus_tag="IW819_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1243425.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(25189..25716)
                     /locus_tag="IW819_000292"
     CDS             complement(25189..25716)
                     /locus_tag="IW819_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1243426.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(25896..26681)
                     /locus_tag="IW819_000293"
     CDS             complement(25896..26681)
                     /locus_tag="IW819_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1243427.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(26674..27543)
                     /locus_tag="IW819_000294"
     CDS             complement(26674..27543)
                     /locus_tag="IW819_000294"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1243428.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(27544..28923)
                     /locus_tag="IW819_000295"
     CDS             complement(27544..28923)
                     /locus_tag="IW819_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1243429.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(28931..29350)
                     /gene="fabZ"
                     /locus_tag="IW819_000296"
     CDS             complement(28931..29350)
                     /gene="fabZ"
                     /locus_tag="IW819_000296"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1243430.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(29375..29851)
                     /gene="accB"
                     /locus_tag="IW819_000297"
     CDS             complement(29375..29851)
                     /gene="accB"
                     /locus_tag="IW819_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1243431.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(29854..31089)
                     /gene="fabF"
                     /locus_tag="IW819_000298"
     CDS             complement(29854..31089)
                     /gene="fabF"
                     /locus_tag="IW819_000298"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1243432.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(31108..31845)
                     /gene="fabG"
                     /locus_tag="IW819_000299"
     CDS             complement(31108..31845)
                     /gene="fabG"
                     /locus_tag="IW819_000299"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1243433.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(31857..32774)
                     /gene="fabD"
                     /locus_tag="IW819_000300"
     CDS             complement(31857..32774)
                     /gene="fabD"
                     /locus_tag="IW819_000300"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1243434.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(32881..33105)
                     /locus_tag="IW819_000301"
     CDS             complement(32881..33105)
                     /locus_tag="IW819_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1243435.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(33158..34120)
                     /locus_tag="IW819_000302"
     CDS             complement(33158..34120)
                     /locus_tag="IW819_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1243436.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(34130..34570)
                     /locus_tag="IW819_000303"
     CDS             complement(34130..34570)
                     /locus_tag="IW819_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1243437.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            34876..35841
                     /locus_tag="IW819_000304"
     CDS             34876..35841
                     /locus_tag="IW819_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1243438.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT        10150 a         7764 c         6259 g        11670 t
ORIGIN      
        1 aagcagataa ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga
       61 cagaactact tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg
      121 tttctgaaaa atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat
      181 acgcaaatag caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct
      241 ttctccaatt agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa
      301 gatcactcga tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt
      361 gacagaaatt tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt
      421 tgtccgaatt tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac
      481 gatatctgta atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg
      541 atgacctgac aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt
      601 atagtcagcg acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc
      661 actgtaaacg atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg
      721 agataattca tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca
      781 acgcttatcg atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct
      841 ttcgtttaat atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa
      901 tctgatttct tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga
      961 ggaaagtttg agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt
     1021 atgccgaaca gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata
     1081 cgccatttgg aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt
     1141 tgcaaatttt tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg
     1201 taatgctcct gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact
     1261 cactgcccgt cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat
     1321 ctgcatgatt tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag
     1381 tgaatcatct gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat
     1441 attttgcata gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc
     1501 tggatctaat cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga
     1561 ggtttcgcct cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa
     1621 tcggatctca tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa
     1681 gttgcgtagt cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt
     1741 atactgggtc agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt
     1801 cttatcgctg ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa
     1861 aatcactgca gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa
     1921 ttgatgcgaa gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt
     1981 tgaccagcgg tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac
     2041 catttttgct gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt
     2101 ttctcgaaca taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc
     2161 tggcatcgta aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg
     2221 atccacatct gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc
     2281 tgcaccggta acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa
     2341 ataatcatcg aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta
     2401 ttcctttgta tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata
     2461 ttctccagct tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg
     2521 gaattcaaat gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc
     2581 cgctttttcg aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag
     2641 tggtaaagat gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat
     2701 cacttcaagt gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag
     2761 cgaataattt tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc
     2821 tgtcaagccg atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc
     2881 gaaaaagtta tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa
     2941 cgctagatgg tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg
     3001 gctggaaagt ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc
     3061 ataatttttt tcttcataag tataactaag atgtaatcca atgattggct gtactccttc
     3121 ttctacacaa atacgataga attcaggtgc accgtataag acatctttat ccgttatggc
     3181 taatgaagtg tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact
     3241 ttgtaaaaga gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc
     3301 cttttttttc gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga
     3361 tagcaggcgc tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga
     3421 gttctttcgc aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga
     3481 aaatctggta aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat
     3541 tctgggctgt cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa
     3601 catatgattt tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga
     3661 ttggtgcagt tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc
     3721 ttgagatttc tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg
     3781 aagaacttta cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt
     3841 cacaataaaa acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc
     3901 taaaacttgt ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc
     3961 ttgaggcgta atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa
     4021 gcctaaaata aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa
     4081 ataacagcaa aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc
     4141 ccctagtttt cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat
     4201 caagccgctg acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt
     4261 caaaataggg ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc
     4321 gatcaaaata gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt
     4381 tttctcgatt ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat
     4441 caacaaagcc cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc
     4501 gcccattgaa ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc
     4561 ccatccgctc ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata
     4621 tccagacaaa atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa
     4681 agccaaagat cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg
     4741 gccttttcga tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt
     4801 cacagagatt gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc
     4861 aacataaact ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc
     4921 aatccacgca atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt
     4981 ctcttttatc atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa
     5041 aatcccgcaa aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag
     5101 aacaaatact gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga
     5161 taatgaataa aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga
     5221 ttactttcac aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc
     5281 acaatcccat aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt
     5341 tgatgtctgc aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg
     5401 cttgatttgg tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg
     5461 ccactactac tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga
     5521 cataagttgt tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg
     5581 caaatagtgc tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt
     5641 cttcttttga gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga
     5701 aagtatcaga taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat
     5761 cactgaataa agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt
     5821 ctttaaatga attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac
     5881 ccataaaaat accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat
     5941 tttttacaac taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt
     6001 tgtcaaagtt ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc
     6061 cgccttcaac gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg
     6121 catttttgat tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa
     6181 gactttcggg aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat
     6241 tgattaatcc gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa
     6301 aaacgaccgt aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta
     6361 catttgcgaa caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa
     6421 atactgcttg gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat
     6481 aactcatggc aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt
     6541 ttttctttaa accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat
     6601 ggaaaactat aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat
     6661 tatatagacg tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact
     6721 taacgttcgt gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta
     6781 ggaatagctg attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt
     6841 tgaggaagga aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta
     6901 ccctcaatca agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca
     6961 atgccggtca tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt
     7021 accaacaatt agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc
     7081 ctaatcagca ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa
     7141 tcttatcgca aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt
     7201 tttttattga cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag
     7261 agaagaattt gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa
     7321 cagacaaacg ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg
     7381 tcgatgttcg gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc
     7441 aaaaagcaaa aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca
     7501 ttgcctttgg tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag
     7561 ctatggctaa cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca
     7621 atcaggaaga cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta
     7681 gattccacaa aaaagaggtt gtgacaattt ttgtcacaac cttttttgcc gagtaaacgg
     7741 tgttcaaaag ccagtttctc ggtatgaagt caaatttaag caaaaatgtg agaagctatt
     7801 ttcacaaaat ttttcttcat actcgaaact aagcggcttt ttcacgacct cttttttatt
     7861 ttgccatgaa catatcataa tacgcgtcca ttatttcttt agcaatcgtc aagtttatgt
     7921 ggtcttgttc gtctaataag ttcggcaaga caacactgat ggcaatttcc ggattgtctg
     7981 ttggtccgta tgccacgata ttactattga ctgtcgcaat atctggatga ccttccgcaa
     8041 ccgtttctgc agtccctgtt tttgcggaaa ggtccatttt cgcactggct aatggtttgg
     8101 ctgttgtgta aggatcggta ccatggacaa cttggtggaa tccttcacgg aggatcgcca
     8161 tattttctgc agaaatattg accgtatttt ctactgttgt gccaatttct ttcttcactt
     8221 cacccaagtt tccattttcg tcattgccat agatcgcttt gactaggtgc ggctgtactc
     8281 gtttcccgcc attagcgact gttgaagcat attgagctaa ttggattggt gtatatgtgt
     8341 caaactgtcc aaatgccaaa tcggtaaagt tacctggagc ataccattct ttgccattat
     8401 ccgcattgaa cttcttcata taatcaacag aacgtgagat cccaggagat tcattcggca
     8461 aatcgatccc agtcgttgta ccaagaccaa actctttgaa agagtctctc aattgctgat
     8521 aagctttagc ttcttcatct aaactaggta gtcccattcc aggtgtatag tctagtccta
     8581 acatctttaa cgccactttg atcatatacg tattagaaga caattccgat gctttgactg
     8641 catcaagagc aacttgtccg cttcgattaa agacagagct tttttctgaa gcaccttgca
     8701 gacgaatcgg ctcatcaatc aaaacttgat ttccgctgat cgcgttactt gcccagcctg
     8761 ctgtcaaagt accagccttg actacagacc ctggatcaaa ggcgctagta atcgtgccta
     8821 aagcattctc ggtgatttct ttggaacctt gttcatgact gaaaccagtc attgctagca
     8881 cttctcctgt ctgaggattc atagccactg cataagcacc aggcgaatac tgtgctttgc
     8941 cgttgttgat caatgtttga taattccttt tcaaaatttc ttctactttt gattggaatt
     9001 ctgcattcat agataacatt aaattagaac ctttttcccc agagaagatt tctttttgat
     9061 tcgaaacatt tccttcatta tcaagggaaa cttcatattg ggttttggtt ccttgcagga
     9121 catcttcata ttgtttttcc aaataacttt gtcctacacg gtcattccga gaataacctt
     9181 ttgctaggta tttctctgct tcatctgctg gaagtccttg cttttcagtc gtcacactgc
     9241 caagaatact tttcagtgaa cttgctgcat tatattctcg tgtccagtct gtgccagtcg
     9301 aaactcctgg aagttcagaa gcgcgttctg ccacaacagc taactcttga tcagtaacac
     9361 cagagttttt gatatagaca gtgtttaatt catatgcact attcatccgt ttaaagatgg
     9421 tagcaatttt taattgctcc tcgtttaaat taatctcatc agctgtgact ttattgacca
     9481 tcgcactata ttgttcgctg ttccctaatt gcttttcttt tgccgaaagt ctttttgtgg
     9541 cttctttcaa atgtttggga tcagccagcc aaaaatcttt tttatctcta tctgtcagat
     9601 tttcatctac cggaacatcg atcagcgagt tcagcttcgt tgcgatttca agcaaatctt
     9661 tagccgtaac ttgtgttcct ctcgtatatg tgatggcctg attcgcttta ttcgtaacta
     9721 aagcttttcc tgtggagtcg tagatcattc cgcgaggagt actttcttgg accgtaatcg
     9781 tagaagtaga cttcactcgc gcctccatct cttcgccgtt cacgatctgc aaataaccca
     9841 atctagcaat caacgcaaca aataacgtaa atataatgaa aaaaagaaaa tttaagcgaa
     9901 aaggaacatg ggaccggcga aacgttttgt tcttaggttc ttgtgcacct tttcttattt
     9961 tttctaacca agaagtattt aatatttttt tcataaaatc tttcatcatt tcctatcctt
    10021 ccgcctattt acattcatgc ttcattgtac ccaaaaactt cttaataaaa aagaaaaaat
    10081 gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt gtttcacagc
    10141 aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact tatttttgaa
    10201 aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg gaaaactacg
    10261 ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg aatccataaa
    10321 atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt cgtttttagc
    10381 catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg aattagttgc
    10441 cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat cggctatctt
    10501 gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca tgcttttcgc
    10561 cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa gccaaagcac
    10621 tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt tttcaaaaat
    10681 gattggctta ccgacacgag accatttttg cagaatatct tctactacta aagaagaggc
    10741 gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga ctttttggat
    10801 cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat tgttaccaag
    10861 tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac cttcatcgat
    10921 tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt gaaaaccgat
    10981 ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg ctaattcatt
    11041 agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca actttttttc
    11101 tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca taagttcctc
    11161 caccctttta atgatagtct atgtaagtat accatatcag acccttattc acggtcgtat
    11221 actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca tcaatcccac
    11281 agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt cggattgctt
    11341 tctatctact acttttttac aggaaattta cctattagtt ttctctgctg taatttttga
    11401 cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt tctttacttc
    11461 cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca actaattggt
    11521 aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca ttacgatcat
    11581 caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa gcataatcgc
    11641 catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac catgttggaa
    11701 aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta gaaacaattc
    11761 cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca tatgtcaaat
    11821 caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct tctgtaccaa
    11881 gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg ccttctgctt
    11941 caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg gctttttccc
    12001 acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag ctcaagcgga
    12061 aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca agcgtacgtt
    12121 taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc aacgtcattt
    12181 ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc atcattccta
    12241 attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg tagaccatca
    12301 tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc atatccattg
    12361 gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac aactcaacat
    12421 ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg tctacgatat
    12481 aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc aaaccagaac
    12541 ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga tggtctcttt
    12601 cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca aaaaaggctg
    12661 tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa gcacctgcca
    12721 ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt ccacgacaca
    12781 aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc aattcactaa
    12841 tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt tttgaaagaa
    12901 cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct tcgattgccg
    12961 gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa aaccctgaat
    13021 cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg gcttgggcca
    13081 tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct ggtgtgatga
    13141 tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt ccgttcactt
    13201 taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt gccaatgttg
    13261 ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt atcataatag
    13321 tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa aaaaatccta
    13381 gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa tgttcaaacg
    13441 caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca agggtttcga
    13501 aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc ctctcttatc
    13561 gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat ttaaccacta
    13621 ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca tttacaccgt
    13681 atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt gcctgcgtgt
    13741 ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc ggactgcgac
    13801 cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct gatcgttctt
    13861 ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg aaaaatggct
    13921 ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac ttcttgcaaa
    13981 acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa aaaaatacaa
    14041 ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct tgaaatcact
    14101 actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc actttcacta
    14161 gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc aagtgctggt
    14221 gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg gatactttct
    14281 aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa ttggactttt
    14341 tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa atttttcaca
    14401 tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg atccgaaaat
    14461 aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt ggacgtttct
    14521 atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt attgactact
    14581 gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag ttggctgttt
    14641 ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc tgcactagct
    14701 gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc ctctacttcc
    14761 aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt acgtgcgagc
    14821 ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg gctcattcct
    14881 gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac taccagtacc
    14941 tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat caaataagca
    15001 atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc aggcttttct
    15061 tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc acgtttttca
    15121 gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg atctccaggt
    15181 ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga agattctcct
    15241 ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa ctggtcatca
    15301 atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg atgggcaatt
    15361 cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt cgcgtaatcc
    15421 tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca gtattctttg
    15481 acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata tcttcctgaa
    15541 cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc agtaaatgcg
    15601 acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa agctatccca
    15661 ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc tcctctttta
    15721 tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta agcatagtcc
    15781 ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac tatcagcgtt
    15841 tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt ttcgattcat
    15901 cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt tatttacggc
    15961 ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt ggatgaaacg
    16021 atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga attgactcag
    16081 gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg ttccaatgaa
    16141 gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct tttgaaatac
    16201 catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa agatagtcct
    16261 tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata tctaggtatc
    16321 gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc accaattgcc
    16381 aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac agatagaata
    16441 gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat tttatctatg
    16501 gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga tgaatcatca
    16561 agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc aatcattcgg
    16621 gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa gacatccgct
    16681 ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga ggctgaaaac
    16741 gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt attgccttca
    16801 ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca gctgctgcca
    16861 gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg ggaaaaagat
    16921 ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg ctatatccaa
    16981 ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg tacgatccta
    17041 tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc tttacatcat
    17101 agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca aaaagacgaa
    17161 acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact atacgcaaca
    17221 gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa actggccaac
    17281 caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta tcaatttgtc
    17341 aatataacga aacaatatct ccactttcta caaactgtgc cactttctct tctaacacga
    17401 cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac acacttgcgt
    17461 caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg ccgaacatat
    17521 ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca ggatttcgca
    17581 ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac tttctgtgga
    17641 gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga agaccttttc
    17701 gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac aaaagaaatc
    17761 actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac agcaagaaaa
    17821 ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa ttggttagca
    17881 gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat catagctaaa
    17941 acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa acaacaagtc
    18001 cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact tccagccttt
    18061 ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc agaacatttt
    18121 tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat gcatgaattg
    18181 acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat ttcatcttat
    18241 gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc agttgtcttt
    18301 ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat ttattctata
    18361 tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat cattttcagt
    18421 tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc aatcccgcca
    18481 ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga ttggaaattg
    18541 ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag gtatgcacta
    18601 tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag aattttttca
    18661 ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg atactcttct
    18721 tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag ataattttgt
    18781 ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt cagataccct
    18841 gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta tttttgcgct
    18901 aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta accgcttaag
    18961 atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt ccatcttcca
    19021 catacttcat atcatttcac atgatactta tgctctcatc ataccacagg ttgaattaga
    19081 aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt cttgcttttc
    19141 gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg ccacattatg
    19201 gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt caaatgtttc
    19261 ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct cagcgattga
    19321 aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat ccgctacttc
    19381 accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga tgtctttttc
    19441 cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt
    19501 gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt tcccaaatgc
    19561 tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa caatcaccat
    19621 tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat ctcctgcacc
    19681 ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag ttttagcgcg
    19741 atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg attttgctgc
    19801 cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat ctttagcagt
    19861 tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct ttctgatttg
    19921 atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg attctgtgaa
    19981 gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat actcggattg
    20041 ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt tgatcacttc
    20101 taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa atagaaatct
    20161 cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt cgaagataat
    20221 ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca taacaagata
    20281 atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca aaatcctcaa
    20341 aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg gtcatctgat
    20401 agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa tacggccaat
    20461 aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga ggcggcacaa
    20521 gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca ctggctatcc
    20581 tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc ggatgtaatt
    20641 ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag atatgatttg
    20701 gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca gtcatttttt
    20761 gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc ctatcttgat
    20821 tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct tctgttccaa
    20881 aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta gcaaaatagt
    20941 cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca ccattttgta
    21001 aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt tgcaaaaaat
    21061 tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc atttccacat
    21121 gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag cttttcatgc
    21181 gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa
    21241 tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt ctgcgacgaa
    21301 aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg ttgaagattt
    21361 attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct acaaagaacc
    21421 agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg
    21481 aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta cgctcttgat
    21541 gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa
    21601 gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa aaagcaaaat
    21661 tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga taggtggagt
    21721 gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca tcaaacactt
    21781 gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag gtaattgcct
    21841 gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg atcgatgcgt
    21901 cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg tctaaccctg
    21961 tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg catgccagtt
    22021 ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac cattgctgat
    22081 aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt
    22141 aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt tccagattat
    22201 agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga tcgatcaata
    22261 cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt tcttcttctt
    22321 tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct gaacagatca
    22381 tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg tcttctgccc
    22441 gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct atgatgattt
    22501 cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt cgattgtttt
    22561 tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc
    22621 tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc
    22681 tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac tcatcttagc
    22741 agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga ttcctaattc
    22801 ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat aattcaataa
    22861 ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg gtcgttcttt
    22921 tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac cgtatttttt
    22981 ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt gttcgctcat
    23041 tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt cattactata
    23101 gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct ggaacaaggt
    23161 agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg atgtcgatct
    23221 caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct ggtcgacgta
    23281 tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt ggcaaatatt
    23341 ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt tgcgtcaatt
    23401 ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc gtttcaatct
    23461 gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt tttgcatcaa
    23521 tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat acgcggtcaa
    23581 gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga acgggattga
    23641 atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc atttcttcag
    23701 gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc actccttata
    23761 ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt ttgtataata
    23821 caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct aatacttgag
    23881 ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc agttctcttt
    23941 tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct tttccgattt
    24001 gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta
    24061 aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca tctacaccct
    24121 ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct agaccggcaa
    24181 aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct
    24241 cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga taaaattcgt
    24301 ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa gcaataaatg
    24361 aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc
    24421 cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg atccgcaaat
    24481 cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt gtatcaatca
    24541 aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg
    24601 aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg ataagcgtcg
    24661 attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg tattcttcga
    24721 ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat aatacatcta
    24781 ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat ttagtttttt
    24841 tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga ttgtcaccga
    24901 caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc accacattga
    24961 cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg tcatccgtca
    25021 atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga caatagactt
    25081 ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt
    25141 tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc attcgattcc
    25201 acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc ttttttccca
    25261 aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta caaggatgct
    25321 acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc ctaccattac
    25381 tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc ttgctaaagg
    25441 tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg cgacacgttt
    25501 tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt ctaaaatcca
    25561 agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat tgtctaaatc
    25621 ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct gatagatggc
    25681 ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat
    25741 gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc aaaaaaaact
    25801 acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa
    25861 gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa cctcttgtat
    25921 cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct ttcaatcaac
    25981 gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt gaacgtttca
    26041 ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat tttcatcaac
    26101 tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa tccttctgga
    26161 gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa acctagagca
    26221 agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac ctttaaatca
    26281 gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca
    26341 ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa cttttccgct
    26401 tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc aaaatttctg
    26461 cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac cggtttgcca
    26521 tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg atcaccatgg
    26581 cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca tcgttcttta
    26641 gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc atcctttctg
    26701 tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg gaacaatctg
    26761 atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg gaagttcctg
    26821 cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc
    26881 tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg tcgggtccgt
    26941 taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag cagagatttt
    27001 tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg aagctgtgaa
    27061 caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga tcttttctcc
    27121 gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa taatgaaggg
    27181 aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg ttttatcttg
    27241 cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg ttacaagatc
    27301 cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct gccatgcacc
    27361 gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt ctttcgtata
    27421 aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag atggcgagtt
    27481 agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct taaacagagc
    27541 catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa
    27601 aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat ccatctgaaa
    27661 ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct gcatcttcat
    27721 taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg aatcataata
    27781 tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca tcccacctgc
    27841 tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag caggattttc
    27901 tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt gacacacatt
    27961 caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc ctgtgaccat
    28021 ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat aaaaatcgcc
    28081 ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg ctttagctgc
    28141 tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta cagaaggaga
    28201 ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc ctaaatgaat
    28261 gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag gataaatgat
    28321 tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct gagctgaaga
    28381 aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc cgccaccacc
    28441 tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca atgcttcttc
    28501 tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt cttgcatcaa
    28561 ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag gaccaataaa
    28621 tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata aaaaaccaaa
    28681 tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt cctgaacatt
    28741 cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt
    28801 atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc ctagttcgcg
    28861 gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata ctttagaaaa
    28921 catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt ttaccgtcta
    28981 ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc tcaacttcaa
    29041 ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc aatccgccga
    29101 aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct cctgcttgag
    29161 ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa tgtccttgaa
    29221 aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct tcaaccattt
    29281 cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct tttatttctt
    29341 gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg
    29401 gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc actccgtcgg
    29461 cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata acttctcctt
    29521 tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt tgcagataaa
    29581 cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat tctttttctt
    29641 tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc tccgaaactg
    29701 gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt ttattcatat
    29761 acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga tcaaattgac
    29821 tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt tcatgcacag
    29881 aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa aacttgcagg
    29941 tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt ctacattgat
    30001 cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc
    30061 ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa
    30121 attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg ctggtgtact
    30181 tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt cttccatcgc
    30241 aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca tatggtaagc
    30301 gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc gagcttgtgc
    30361 atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa aaccattccg
    30421 attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga gtgctgttaa
    30481 agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc cagcaagaat
    30541 gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat tcgttccgct
    30601 agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca aagcaatatt
    30661 tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc gttttgcacc
    30721 tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga tcccagatcc
    30781 gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc tcatctccat
    30841 tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt tgctatcttt
    30901 tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg taatccctgt
    30961 ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt ttaagctatt
    31021 caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag taattaccac
    31081 tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc catcgacatt
    31141 gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt gtgcaatatc
    31201 ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct tgactttgtc
    31261 tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat tacatgtaat
    31321 gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc cagctttact
    31381 agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag agagattaat
    31441 gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca tattgaaggt
    31501 tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc gcatcacaag
    31561 tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa gcttttcttc
    31621 tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg aaactcctac
    31681 gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct ctccgcgacc
    31741 attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt tgccgattcc
    31801 tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt tcctccttat
    31861 agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac tggtattgtt
    31921 ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc gatgacttgt
    31981 tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg ttgcatgact
    32041 tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt tgtgttgctt
    32101 atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc cagttttttt
    32161 gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg gatcattctt
    32221 ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca
    32281 atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta cttgctagct
    32341 tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac cattttcccg
    32401 cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa cgcaactgct
    32461 tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa actcagtcct
    32521 aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc gatgctgact
    32581 gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc tgtaaagcaa
    32641 agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg ctttacaacg
    32701 gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc actaaataag
    32761 aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt cttcctattc
    32821 aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta attttaatga
    32881 ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga ttccgtcttc
    32941 tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga ataaatccaa
    33001 gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt cagtttcttt
    33061 tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa tatattcctc
    33121 cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg agaatacttc
    33181 cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt cctaaggtga
    33241 tctttccact agaaactgct tcatccaata agatagggat gcttgcagca gatgtattcc
    33301 catatttatc catattggtc aggaactttt cttgcggcat ctttactttt cgggcaattt
    33361 tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt ttgtctttca
    33421 tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata tctctgccag
    33481 tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt ttattttgct
    33541 ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt tctttcagca
    33601 attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca aacaacacag
    33661 cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca atcaccagtc
    33721 ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa acaaatcctg
    33781 agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc ccttgtactt
    33841 gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca
    33901 aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa atgcataaat
    33961 cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt gacgaaatcc
    34021 attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc ttttcaggaa
    34081 ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct cacttatagt
    34141 cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt tcctccgtag
    34201 ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg acccgaaata
    34261 gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct tcactgcgga
    34321 tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag gtcccaacag
    34381 taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc ccgattgcct
    34441 cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc agctcagact
    34501 cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact gtttttaaat
    34561 ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt gatgttcaaa
    34621 ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac tatatgagct
    34681 ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa tttcttttat
    34741 tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg aacagaacta
    34801 tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt tattcaaagc
    34861 gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc
    34921 tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa
    34981 aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt attacgtatg
    35041 attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga tgctttattg
    35101 ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat gaattatttc
    35161 agtaattata attatgtaca tattaccaat aagggatttg tattaacaga tactcctacg
    35221 gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt gcgtataatg
    35281 gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt gcgagtccag
    35341 cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca agggctgttt
    35401 caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca tttcatctat
    35461 cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt tacttatttt
    35521 attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac cttcagcatg
    35581 ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg tcaacttgca
    35641 gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa gaaagaagct
    35701 catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca aatcttgatc
    35761 agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa caaaaaagaa
    35821 gctgaaaagc aactaaatta gtt
//
LOCUS       DACEPW010000008        33705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEPW010000008 DACEPW010000000
VERSION     DACEPW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.008"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..519)
                     /locus_tag="IW819_000305"
     CDS             complement(<1..519)
                     /locus_tag="IW819_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HAQ1243439.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEK"
     gene            complement(781..1488)
                     /locus_tag="IW819_000306"
     CDS             complement(781..1488)
                     /locus_tag="IW819_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1243440.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(1509..2291)
                     /locus_tag="IW819_000307"
     CDS             complement(1509..2291)
                     /locus_tag="IW819_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1243441.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(2340..2627)
                     /locus_tag="IW819_000308"
     CDS             complement(2340..2627)
                     /locus_tag="IW819_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1243442.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(2642..3241)
                     /locus_tag="IW819_000309"
     CDS             complement(2642..3241)
                     /locus_tag="IW819_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1243443.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(3255..3932)
                     /locus_tag="IW819_000310"
     CDS             complement(3255..3932)
                     /locus_tag="IW819_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1243444.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(3949..5190)
                     /gene="ftsZ"
                     /locus_tag="IW819_000311"
     CDS             complement(3949..5190)
                     /gene="ftsZ"
                     /locus_tag="IW819_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1243445.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(5213..6538)
                     /gene="ftsA"
                     /locus_tag="IW819_000312"
     CDS             complement(5213..6538)
                     /gene="ftsA"
                     /locus_tag="IW819_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1243446.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(6694..7914)
                     /locus_tag="IW819_000313"
     CDS             complement(6694..7914)
                     /locus_tag="IW819_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1243447.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESSQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(7929..9017)
                     /gene="murG"
                     /locus_tag="IW819_000314"
     CDS             complement(7929..9017)
                     /gene="murG"
                     /locus_tag="IW819_000314"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1243448.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(9043..10404)
                     /locus_tag="IW819_000315"
     CDS             complement(9043..10404)
                     /locus_tag="IW819_000315"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1243449.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(10418..11380)
                     /locus_tag="IW819_000316"
     CDS             complement(10418..11380)
                     /locus_tag="IW819_000316"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1243450.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(11406..13598)
                     /locus_tag="IW819_000317"
     CDS             complement(11406..13598)
                     /locus_tag="IW819_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1243451.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(13599..14000)
                     /gene="ftsL"
                     /locus_tag="IW819_000318"
     CDS             complement(13599..14000)
                     /gene="ftsL"
                     /locus_tag="IW819_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1243452.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(14005..14964)
                     /gene="rsmH"
                     /locus_tag="IW819_000319"
     CDS             complement(14005..14964)
                     /gene="rsmH"
                     /locus_tag="IW819_000319"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1243453.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(14985..15416)
                     /gene="mraZ"
                     /locus_tag="IW819_000320"
     CDS             complement(14985..15416)
                     /gene="mraZ"
                     /locus_tag="IW819_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1243454.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(15586..15954)
                     /locus_tag="IW819_000321"
     CDS             complement(15586..15954)
                     /locus_tag="IW819_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1243455.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            16125..17081
                     /locus_tag="IW819_000322"
     CDS             16125..17081
                     /locus_tag="IW819_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1243456.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17154..17270
                     /locus_tag="IW819_000323"
     CDS             17154..17270
                     /locus_tag="IW819_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1243457.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(17325..19007)
                     /gene="recN"
                     /locus_tag="IW819_000324"
     CDS             complement(17325..19007)
                     /gene="recN"
                     /locus_tag="IW819_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1243458.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(19025..19474)
                     /locus_tag="IW819_000325"
     CDS             complement(19025..19474)
                     /locus_tag="IW819_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1243459.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(19581..20399)
                     /locus_tag="IW819_000326"
     CDS             complement(19581..20399)
                     /locus_tag="IW819_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1243460.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(20408..21298)
                     /locus_tag="IW819_000327"
     CDS             complement(20408..21298)
                     /locus_tag="IW819_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1243461.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(21300..21524)
                     /locus_tag="IW819_000328"
     CDS             complement(21300..21524)
                     /locus_tag="IW819_000328"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1243462.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(21529..22866)
                     /locus_tag="IW819_000329"
     CDS             complement(21529..22866)
                     /locus_tag="IW819_000329"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1243463.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(22867..23721)
                     /locus_tag="IW819_000330"
     CDS             complement(22867..23721)
                     /locus_tag="IW819_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1243464.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(23818..24267)
                     /gene="nusB"
                     /locus_tag="IW819_000331"
     CDS             complement(23818..24267)
                     /gene="nusB"
                     /locus_tag="IW819_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1243465.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(24260..24685)
                     /locus_tag="IW819_000332"
     CDS             complement(24260..24685)
                     /locus_tag="IW819_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1243466.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(24931..25995)
                     /locus_tag="IW819_000333"
     CDS             complement(24931..25995)
                     /locus_tag="IW819_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1243467.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(26167..26412)
                     /locus_tag="IW819_000334"
     CDS             complement(26167..26412)
                     /locus_tag="IW819_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1243468.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(26551..26925)
                     /locus_tag="IW819_000335"
     CDS             complement(26551..26925)
                     /locus_tag="IW819_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1243469.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(27008..27922)
                     /locus_tag="IW819_000336"
     CDS             complement(27008..27922)
                     /locus_tag="IW819_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1243470.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(27943..28746)
                     /locus_tag="IW819_000337"
     CDS             complement(27943..28746)
                     /locus_tag="IW819_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1243471.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(28779..29768)
                     /gene="manX"
                     /locus_tag="IW819_000338"
     CDS             complement(28779..29768)
                     /gene="manX"
                     /locus_tag="IW819_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1243472.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(29871..30362)
                     /locus_tag="IW819_000339"
     CDS             complement(29871..30362)
                     /locus_tag="IW819_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1243473.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(30553..33387)
                     /locus_tag="IW819_000340"
     CDS             complement(30553..33387)
                     /locus_tag="IW819_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1243474.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
BASE COUNT         9266 a         7407 c         5627 g        11405 t
ORIGIN      
        1 tttttcagcc attttaccaa atacgcgtac ttgtgcagct tcataagaag gatctaatgt
       61 aacataagga tgttcccaat ctcctgcaac tcctaggcgt ttgaaatctg ctcgttgctt
      121 atctacttgg gaaagtgcat attcacgaca tttttccaag tattctgcca tggacatttc
      181 tttacgtttc acgcctttat tagccaatac ttgttcaatc ggcaatccat gtgtatccca
      241 tcctggaaca tatggtgcac gatagccaga cattgatttt gaacgaatga taatgtcttt
      301 actgatttta ttcaatgaat gtcctaaatg gatattacca ttggcatatg gaggtccatc
      361 atgaagaaca aagctaggtt ttccttcgtt caactcttgt cttttttggt agattcgatt
      421 ttcttcccat tctttttgcc actcgccttc tcggtttggc aaattaccac gcatagggaa
      481 ttttgttttt cctaattgca aggtttcttt cattttcatt caaccataca ctcctttatt
      541 tgttttccgg acacaaaaaa gcatgttcag ccgcaaggga cgaacatgcc gtggtaccac
      601 ccaaatttag ttgcatctat ctagtgcaaa ctctctgtga ccaataacga ggtcagccgc
      661 tgcttcttac tctaatttcg gaaaagcagg tctcaagtag aggatctttc accatgcagt
      721 gtcttaccga acttccactc tcatcggctc gctttaagaa taacaaagtg cttttctgtt
      781 ctactcgttt tcattttcac caggcacgat cgagatatca atagcctgtg tatcaaatgc
      841 cgtcataggt gcttctatga ctcctgtgtc tgagtttaca tcagtatcat tttcattgtc
      901 aagctgtttt gctaaaactt ctttgatcac ttcatggctg tcagtcacgt agctggagaa
      961 tggttgtaaa atttcatccc attccggact tttcacttgt tctaattgag actcaagcat
     1021 caagctgata cgttgatgga atactcgtgt ttttttctta agatcttcag tttctgtcgc
     1081 aagctgtctt gctttttctg ttgcatctgt caagattttt cttgcttttt cttctgcatc
     1141 agttgttaat tgatgtgcac gtttttcagc gttcgcaact aactcatctg ctttgttttg
     1201 tgcagaagta acgatcactt cggattcctt gcttgcactt gtttttactt tatcagccgt
     1261 atcttgagct acgatgattg attggttcaa agcatctttt aattcattga agtactcaag
     1321 tttttcttca gaatgtttga ctgctttttc taattctcga tttctttgag tcaattcctc
     1381 atagtcacga acaactaaat ctaagaaatc atctacttcg tcttgattat atccccgcat
     1441 ttttgtcggg aatgtcttat tttggatatc taatggagtt aaagccatga gtaaaccacc
     1501 tttttatttt atttacgtaa tacaccgaac agtaccctgt acttttcttt tttcgtcttc
     1561 ccctcaagtt cttggatctg gactctgcca taacctctga ctgaaataat atctaatagt
     1621 tcaagtacaa aatcaggtct tgtattttct gtccagttca cttttacttt accagcttcg
     1681 atcagctgct tggaccgctg tctggaaata ttataaatct cagagatcac actgtctagt
     1741 cgcatggaac taactgttcc tctttcttgt gtccagctat ctttcggcat aatcatttct
     1801 gtgtatttga ttggttccaa gcgtacagca acctttccaa tctttgttac ttgtgcagca
     1861 acaaaatgat ccacctcatt tgcaataaat acttgccagc gatcaccgtc cgagatgata
     1921 tcgccaaagt aagagcgttt gatccccgtt cccactaaag ttccaaggat tttcccatga
     1981 cttaggctgg caaatttgct aggataatgg atttcataca atgctgtttc aaaatcttcc
     2041 tctacaggtt cgtaataatc ggggaaaatc aaacatcttt ttctttctgc ctgttcatat
     2101 ccgccataaa acatgaaacg caaatcactg ctttgtcgaa tcagtgtttc taaaatatag
     2161 gattgtcttg ggtcaagaaa ttcggtcaga tagggtgcat attgtccttg aacctgttca
     2221 atccaatcgc ctaccgaatc gataaatgta cgttcatctg cccgaaagtg ctggtataca
     2281 tttgcatcca aaattttccc ctcctatcta ttctgagaat aaagattttg ttgcttcaat
     2341 caataaacta aatggacagt tatccatata acgatctgtg ctaaaccttg tacggctaag
     2401 ttcaagatca ggattgcccc taaaattgtg aaatccaagg gtccaatcct caaaggcaga
     2461 cgatcgaaca aactgatata cggttcgcag atcttagcta ataatcgtcc tagccctgat
     2521 tgatacccgc ctggaaacca agagagcaat gcatatacca tcaagataat gctatagaaa
     2581 taggctgccc gttgaagtaa atgaagtaat agtgcaatag tataaatcgt agttcctcct
     2641 cttacagatc aaacaaattc gcatctgcaa aagattgagc ggttgaactg tcaatctcca
     2701 tcccagtcgg tgtacaaaga aaaatctcat ctccaacacg tttgatatct ccatctagtg
     2761 cataaactgt tcctgttaga aaatcaacga ttcttctcgc ttgtttttct tctactaaat
     2821 gaaaattcac taaggttgct tctccagcaa tgattcgttt agcaatcgcc atagcttcag
     2881 aataagctct tggttccatt acagtgattt ttccagcaag atttgcttta ggttgtgtag
     2941 aggttcttga agctgttctt tctttcgcta cagtttttgt aggtgattga tgtagttcaa
     3001 ttactttttt cttttcagcc ggctgaatac tttcatgcgt tacaggacgt gaagtcgccg
     3061 ttttttgttt ttcatgagta ttcgcagcag atacagaatg agttgattga ttctgtgttg
     3121 tatttttttt agttggctgt acagcctttt ttgttgtttc aggataatct tggtattcgt
     3181 actcttcctc atcagctaat ccgaaaaaat tagcaattgc tcctctgttt aaaagcggca
     3241 tttttgttcc tcctttattc gtctttgaat agcgctgttc ctacacggat aaatgttgca
     3301 ccttcttcaa tcgcaatttg aaagtcttga ctcatcccca tacttaattc actacatggt
     3361 gcatgggcat aaccttttgc ttgaacaaga tcacgcaatt ctttcagcgt accaaacact
     3421 ttatggattt gctcatcaga agcttcttta ggcgccatag tcatcaaccc aacaaccttg
     3481 atttttgtgc aggcagctaa agattccaca aacggtaaaa gctcatctgg tgaaattcca
     3541 tgttttgatt cctcaccaga aacatttact tcaataaaac aggataattc tttctctaac
     3601 cgcttctgga tttcttctgc taatcttaaa ctgtccaacg catgaaagta atcaatttca
     3661 tttacaatca attttacctt tctgcgctgt aaattaccaa ttaaatgcca ctttatttca
     3721 ggaaaatcaa ataaatcttg tttcttttct aataatttat ctactcggtt ttctgcacaa
     3781 ttcataacgc cagctctaac caattcttca gcttgagcgg attcaacaga ttttgttaca
     3841 gctactaatg taacttcttt cggatcacga tgaacaagag cacacgaatc ctgaatttct
     3901 tgcttgattt tctgcaagtt gtcagcaatc atgtgctctt cctctccttt attttttacg
     3961 acggaaaaac ggtggcgtat ttaattcatc atcgccatga gattttgttt cttcgcggtt
     4021 gaatgtatca aattcttttt tttcgatatt gtcaaaattg gaatcatcaa ctctaggacg
     4081 aacattttgt tcacgtctga tatcccaatc gccaaaactg ttttcttctt ctgaagaagt
     4141 tggtttagct tgatccatat ctaatacagt cttttgagca ggtgattgca tttgtgcttg
     4201 tctagctggt cggctagact tgcgttcctt ttttgattca tcgatacctg ttgcgatgac
     4261 cgtcacacgg atttcatcgc ccatttcttc attgatagat gtacctagaa tgatatttac
     4321 atcgccagtt gctgcatttg ctacgatatc tgaagcatct tgtgcttcga acaatgtcat
     4381 atccaaaccg cctgtgatgt taagaagtac ttgttcagct ccatcaatag atgtttcaag
     4441 caatggagag gagattgctt ttttcgttgc ttctactacg cgatcttctc cactagccac
     4501 accgatcccc atcaatgctg taccttggtt ttccatcact gttttcacgt cagcaaagtc
     4561 aaggttaacg taacctggcg ctgtgatcaa atcagagata ccttgtacac cttgtcgtaa
     4621 aacattatct gcttcacgga aagcttcaag catcggtgtc tttttatcta caacttctaa
     4681 taaacggtta tttgagataa ttaataatgt atccacattt tctttcaaac gagcgatacc
     4741 ttctgcagca aaacggccac gtttcggtcc ttcaaaagtg aatggacgtg tcacgacacc
     4801 aacagtcaat gcgcccaatt ctttcgcgat acctgcaacg attggtgcag cacctgtacc
     4861 ggttcctcca cccattcctg cagtgatgaa aatcatatct gcaccgtcta aagcttcgcg
     4921 taaggattgt tcactttctt ctgccgcttt ttgtccaact tctggctgag aaccagcacc
     4981 taaaccacga gtatatttgg ggcctaattg gataactgtt tcagcttttg aattttttaa
     5041 tgcttgcacg tctgtgttgg ctgtaatgaa ttcaacacct ttaacgtttt cttcaatcat
     5101 tcggtttaca gcatttccac cgccaccacc gactccgatg actttgatta ctgcgccgtc
     5161 attgatatta ttatcaattg agaattccat atcttattcc tcccaggatg aattaatcaa
     5221 aaatatttgt aaagaaaccc ttgattttat tttttacatt ttctccggac tcttttggtt
     5281 ccggctcatc gtagattgtt tcttcaggtg cttcatagct ctcatatgtg tttgttgttt
     5341 cttgttcaac taccatttga cgagctgctg aggtttcacc cgtaacagca atttttgcca
     5401 attgatacac ttcactcaag tttgctgaat aatccacgat acttattaca tttgtaaata
     5461 ccgggtttct cagccccatg tgatttggaa catacaattt tacatttacg ccgaagattt
     5521 cttgtgccaa gtccacaaca ccaggaagac tagcagctcc gcctgtcaat acgacgccac
     5581 ctggcaactc taatgcttcg atttggtcca atgcatcttt tgctttgttg aaaatctgtt
     5641 ccattcgagc tgaaataatt tctgacaagt agcgttcatc gactttcact ggttctgact
     5701 gaccaatcac atctactgga aattcttcat ctggtgatgt ccgttctggg tatgcatccc
     5761 cgtagtttat tttcaacgct tctgcattgt taaatgacgt atttaataca atagagatat
     5821 ctttagtgac aaattcgcca ccttcttggt caagattggt aaacttcaat tgcttatcat
     5881 gcataacagc cgttgttgtt tggccgccac ccatatcaat gacgatcgta ccaaaatctt
     5941 tttctccgtc agaaagaatt gattcagtca gcgctaaagg agtaatcacc atttcgttga
     6001 cgattaatcc ggcattttcc acgcatttac gtatgttatg gatgatcgtt ttaggtcctg
     6061 taaacaacag accgtacatt tctaatctta cgccgatcat tccacgagga tctttgattc
     6121 cctcgaaccc atcgacagta aaatcttgtg gcaatattga cacgatttga cgttcaggag
     6181 gtattgaacg aacaagggca gctgaagcca catttctgac atcttcatcg gtgatttcct
     6241 ttgaatctcc gttaactgca atcattccct ggcaattttc tacttcaagc atgtttgctg
     6301 gcaaaccgac acttacgcct ttgatctgga tacctgcttt ttcttctgct tgtcgtactg
     6361 ctcgttgtat cgcttggacc gttttatcga tatcaacgat aatgcctcgg ttaatccctt
     6421 ctgattttgc gtttcctact ccaataatat tcatttggct gtcgatatat tcagccacga
     6481 caactttaac agatgtcgtt ccaatatcaa ggcctacata cattcctgtt tttgccatga
     6541 atgggattcc ctcctactta ctattccata tacaagcgaa caactagcgt ccgttttaaa
     6601 atatacttat taatagatat attcactgct tccaattcta ccataaatta ctggttttta
     6661 gaaagtacat tcttactttt taaagttgtt ttatcaattt tcatttaaac ttgacggagt
     6721 tgtacttgga ttatcttcta tttgacttgt ttcacttgaa tcatttgaat cagatgatcc
     6781 gtttgtgcta gacgatccag gtgtttgtgc tttttgcgtt tctgcctctt cacttgattc
     6841 gtttgtttga tccgcatttt cttcactttc agttgtttct tccgattggc tcgattcttc
     6901 cgtttcttga gtgctaccgt aaggatgcga gaagatacca acttccatgt cgatcacacc
     6961 tttttcatcc atatctttgg ctacttgcga ataatatttc atttggttcg caagattatt
     7021 gatatttaca ataacttgat tgccgtcatt catattcaat tggatcagat ttttattgct
     7081 tgctcttggc gtgtacttga tttcagaaat tgctttttgt aattctgatg ataactgatt
     7141 gtattgcttt gttaattctt tgatttttgc agagtctttg aactcttcca agatagggag
     7201 attttttgta ggatttgcta ctttttcatc taatactgtt ccattttcaa tcactgggta
     7261 gtattgattg ttttttgcga tcaaagcaat ctctttatat tctgttacat ccaaatcgaa
     7321 ttcattcatc cctttgaaat gtatatttgc tgtttcgatt cttggctgtg cttttttaag
     7381 gcgatcgata taagtagatc gatgaaaata ctgtccccaa acattttctc ctaatgacaa
     7441 ttttgtatca gagatgattt cttggctgtt gactgtctta ttgcctgata ctgcaactgc
     7501 ttgtaatttg ctataaggag aaacataata gatcaaaaaa atcagcggta tacccaatac
     7561 agtgatgatc aaagctaacc gacgaaatag tactttgtgc cgttgacttt ttaaattggg
     7621 cagccggttc aaaaaagaac cgttatatgg tccaccgttt ttcttttgct tgatctcttc
     7681 aaaaaattcg atttttttct cggactggct ttcttcttgc tgttcctctt gaaccgtttt
     7741 ttcttcttca gaatctgaaa ggggtgtatc taatgttatg tcttcttttt ttggctcttg
     7801 gtttttcatc tcttgttctg ccagatattt tcggttagcc ttttgccaag gagtcaaatt
     7861 gttttcatca gaagacggat gagacaattt ttttggttct tttttcttac tcactaagac
     7921 caccgccttt attttttgat tatttgtttt actaagtcat aaagacgttt tgaagcatca
     7981 gggattcctt gttctttaga agctcggcac atttgctttt gcaattcttc atcacccatg
     8041 atttcgttga ctgtctgact tagtgattca cctgttagtt cattatctgc aatcattttt
     8101 gctgctcctg cgtgaacaag actcattgca tttttagtct gatgatcatt cgtaacatat
     8161 gggctgggaa ccaacactgc cggtaacccc agagctgtga attctgcgat cgaagttgca
     8221 ccagcacgcc ccaccagcag atcactggaa gccatgacct ctgccatatt tttgatataa
     8281 ggctggacac taatattttg tatttcagga tttattttat ttaggatctc ttcataatat
     8341 ctatctcctg aagcatacaa aacttggtaa gaagaaggaa tatattcact taaaaaaact
     8401 gatactgctt gattgatttt aagtgctcct tgactaccgc caaaaatcaa gactgttttc
     8461 ttttctggat ccaaatcaaa tgttttcaaa atatctgatt ttttagtatc tgctacttct
     8521 tgtgctcgag gattacctac aagaactgct ttgttttcag gaaaaaagga agccgcatct
     8581 ttaaatgaaa gagcgatccg atccacatat cggcttaaaa atttattggt aacacctggt
     8641 acactgtttt gttcatggat aactgttgga atacccattt tggctgcagc gtacactacc
     8701 gctccggaaa catatcctcc agtaccgata acgatatctg gttgaaaatc actgaggatt
     8761 ttttttgctt gacggatact cttaagaaac aatcggaccg tcttgatgtt atgcatggaa
     8821 atttttcgtt taaatccttg aatctccaag gtatggaaag gcattcctgt atcagggacg
     8881 attttgtttt ctagacctcg ttttgctccc acatacatga attcagcatt cggttcttga
     8941 gtctttacat aattgacaaa ggcaagagct ggataaatat gtccccctgt tcctccgcct
     9001 gtaaccaaaa ttttcatttt ttatcgctcg cttctacttt ttctatttta atcggttcac
     9061 agcttccatg aatctgtttc ctctagtttc aaaatttggg tattgatccc agctggcatt
     9121 cgctggtgat aataaaacta catcaccatc tgtactgttt tctaatgcta aaggaactgc
     9181 ttgttctaca gaatcggctg ttactattgt ctcgatgcct gctttttttc cagcatccat
     9241 caacttttct tttgtttggc caaatacgac cattgctttg attcctttta atgaaggaat
     9301 taattcatca aatgtatttc ctctgtctaa tccgccagcc aataacacga ccttagacgt
     9361 ttcaaatcca cttagagcca ttttagtcgc taaaatattg gttgcttttg aatcattata
     9421 aaattttctg ccttggattt ctcctacata ttgcgtacga tgtggtacgc catggaaaaa
     9481 atgcaacgtt tctcgtatag cttcatttga tataccatac aattttgcta cagaaatagc
     9541 agctagtgca ttttctacat tatgcttacc tggtacgcca agttcagata tttccattat
     9601 tttttctttt ttatagtata ttgagtaatc gtcagcatac acaccgtctt ccaacacttc
     9661 ttttgtcgag aaaggcagta cgcgtgcttt tgttctttgc gctaattctt gtagttctga
     9721 ctgattccag ttaaggatca gataatcttt ttctgtcatg tttttttgaa ggttccattt
     9781 agctttgaca tattcttttc ttgtaccatg ataatcaata tgtgcttcat agatattggt
     9841 aatcaccgct atatgagggc ggaaatctgt gattcccatg agttggaagc tagacaattc
     9901 catcacaatt ttatcttctg attttgcttc ttgcgctaca ccacttgctg gatacccaat
     9961 attacccgct aaacgtgcaa ttccttgatc tgctcctgca ttcagtagta gtcctgtcat
    10021 cgttgttgtc gttgtttttc cgttcgttcc tgtaatccca ataattgggc attccgctac
    10081 ttcgtaagct agctcaactt ccgtaattac cggaataccc atttcttggg cttttgctac
    10141 taaaggatga ctgtagggaa tccctggatt tttgaccatc aatgaaaatt cttcatccaa
    10201 caattcgatt ggatgactac ctgttattac ttttatccct aaagaaagca gctcttgtgc
    10261 ttccggattt tcgtcaaaag gttttccatc attaacggtg acaagtgcgc ccaattcatg
    10321 taataatttg gccgcactta ctccgctttt tgcgagtcct aagacaagca ctttcttatt
    10381 ttcatatgtt gatatttttt tcatttcccg tcatctccta ccaaacgatc cataatgtga
    10441 tcaacgaagt cacaatgctg atcaaccaaa aaactgtatc aatcttccac tccgaccatc
    10501 cgcacatttc aaagtgatga tgaattggag acattttaaa aattcgtttt cctgtaagct
    10561 taaaggaagt aacttgaagc atcacacttg ctgtttccat aacatagatc aaaccaatca
    10621 aaagcaatgt ccattcttgg tttagcatta tagaaattgc cgctaaaagt ccgcccaacg
    10681 ctaatgaacc gacatctccc ataaaaatct tggcaggttt tctgttgtag acgaagaaac
    10741 ccagtaaacc tcctaaaaca cttaagcaaa tgattaatac atcatactga tgctgatgcc
    10801 aagcaattag accgtaagct gcaaaagaga tacttcctaa gccagcaact aatccatcga
    10861 tgccatctgt cagatttact gcatttgaaa atccaacaag ccagaagaga gcgaaaatac
    10921 cataaatgat tcctaaaggt agatctagtc cgaagaaatt caatacacct ggataacctt
    10981 cagaacggta aacaagataa aagatgattc cccctagaat ctgtccaatc aatttctgct
    11041 tcgagttcag tcccatgttt cttttcttga atatttttat aaaatcatca agaaagccga
    11101 ttactccgta taacgctaat acaaaaagca agatgaaaag agtaggagtt agctgttttt
    11161 gccaagctcc aacccaaatt cctgtcaaaa tagagccgat cagaaacact aagccgccca
    11221 ttgtaggagt tccggctttg ctattgtgcc actttggtcc ctcctcacga atcgcctgac
    11281 cttgcttttt catttgaaaa taaccaataa atagcggcat tgcggcaatt gtcattgcac
    11341 aactgctgac aatgggaatc aatgcttgtg tccattccat gctcttttct ctcctatttc
    11401 tcttcttatt cttctaaagt aaaactaagt ttgtcttcag tgattttttc atatggagca
    11461 agttcttgtt tgacacaata accatctcca tcaaaactaa cttcaatacc gaggattttc
    11521 cccagcttca tgatatctgc ttttgaccat cctgttacgt caggcatcag cttatcacca
    11581 ccagtatata ggatcagttt ttctccagag atcaactgat caccgttggc agtagattgt
    11641 ttttgtactt ttttaccatt accgatcact acaggttgca gtccactttt ttgagcatca
    11701 gctgctgcaa cgtccgcttc aagattacga taatcggcta cggatacttt ttctgttttt
    11761 gtttctgtat ctgaatcttc ttcagtttct ttaaaatcca ttgcccgttt catcaatgga
    11821 tttgcgattt tagccaatgc cattcgatca tatgtcttag ggtgtttcat tgttagatac
    11881 aagacatagt ctggatcttc agaaggaacc atctccacaa tcgaatacag gtaggctgta
    11941 tctcctgttt gatatccccc tgtatcagaa gcgatttggg ctgttccgtt ttttgccgat
    12001 acattgtatc caggtacact atatacacca taagcactac cgtagttttc gctttcgaca
    12061 acatctctca tatattcacg gactttttct gttgtttctt ttgaaaaagg ttgccctaga
    12121 acttctgttt gagttgttcg ctcttctcca gtttggggat ccaccacttt gctgatatat
    12181 cgaggttgaa tcattgtacc gttattcgca atagaagtga aagctttcat catttggaaa
    12241 ttcgtcacac ctaccgcttg tccataagca ctcattgctc ggtcgacaat atttaatgta
    12301 ggtaaagcac cgtttacttc atcatctaat ccagaatgag tactttgtcc aaatcccagt
    12361 ttttgtaaat agttatacca tcgtccgcct aatcgctgtt ccagtatgac catcccgacg
    12421 ttacttgacc atgaaagagc ttgacgcatg gtcagcacac ctttttcacc aaagtcatgg
    12481 tcattgatcg tagcatcttc tacttggatt tttccagatt ggaaggtttc attctcgttg
    12541 aattctcctt cttcgatagc agctgcagta gtgaaaactt tcatggttga tcctggttca
    12601 taactatctt gtaccaagaa attacgccag atagcatctt ttccagtcaa tccttccatt
    12661 gtttcaggat tgaaagtagg tcgttgtccc attgccaaga tttctccggt ttttgctttc
    12721 atcaggactg ctgtcaattc ttctggctga tattcttcat ttacttgatc catcagtgtt
    12781 tctaaataac tttgtagtct gctgtctaaa gtcgtgtaga tatcttgtcc gtcgacagct
    12841 ttttcttctt ctgccactgt acctggcaga ggattttgaa aattatcttt ttgataaata
    12901 atcttaccat ctttaccgct caagatatca ttgtaagcag actccaaacc tagtttacca
    12961 actaagccat tctcatcttt gtctggaact gcgtatccaa taaaatgaga agaaaagacg
    13021 ccatttggat acatccgagc ctgatggtcg acaaaataaa gaccagcgac accttctttt
    13081 ttcatgtccg cttcgatgtt ttgcttggtt tcttgggaga tatttttccc atagctacca
    13141 aattcaactt gatacagctc tttttttgcg ccgtcctcta aaatattcag tgcatctttt
    13201 ttgtctatag aaagattctt atgcaggatt tctgctattt tatcaaaatt cttttcttca
    13261 acatacagtt ttttatcccc agaggtatat gtttttgaga gtatcgcttt aattgaataa
    13321 gaggaggcat cttctgcgag cgccacaccg ttacgatcat aaatcgttcc tctttttgct
    13381 tttactactt cacttccttg gtacaactgt tttgtttttt cagctaatga ggtaccagca
    13441 acatgacctc caataacgat atacgagaaa cggacggcaa ataaaaagaa caatccaata
    13501 ctcgtagcaa ataaaatgat acctactttt ttgcgattgt tcatcggatt tagatttttc
    13561 ttttccataa accgccgaat tttattttta agactcattt acttcacttt ccttaaattg
    13621 tcgtcattaa tagacaatcc tttttcttct gcgatttgtt taatccgttc agtacgagaa
    13681 agttcgtttt tttcttgttc taaactcgtt ttttcttgct ttttatcatt gatttctgtt
    13741 tgtattgacg tgatttcttt ttcagctccg ctgatatttg tgcgcagcat aactgttaaa
    13801 acacataaag caacaatagc aatcgccacg cttgccacag caattttttc caaagcagaa
    13861 atctgcttta atcgttttgc cggtgattgt ggaagaatga tttcttgatt cgaagaagaa
    13921 tcaggcattt gttgttccgg ctgatccacc aattcaggta aatcatattc gtaatcttgc
    13981 attttcttta attctgccat cattctactc ctctttcatt cttatttttt ctgcaatacg
    14041 taactttgca ctgcgtgatc gattgttttc tgttagctct tcctctgaag gaaggattgg
    14101 tttacgagta atgactttca gttctggttg gaattcgtca ggtatgacag gaagtcccgg
    14161 tggtagatct tgtaccgtac tatattcttt aaacatactt ttgacaattc tgtcttccaa
    14221 tgaatggaat gtgattacgc tgattcttcc attgactttc aataaccgga ttgcctgttc
    14281 taaagaggcc tcttcggcac cgagttcgtc atttactgca atccgcacag cttgaaaaat
    14341 tctttttgca ggatgcccgc cttttcttct tgcgggagct gggatgactt gtttaatgat
    14401 ttcgacaagc tcaccagttg tttggattgg ttttgttttt cgtactcgtt cgatttcacg
    14461 agcaatttgt ttggaaaatt tctcctcgcc atagcggaag aaaatcttta ctaattcatg
    14521 gtaggaatat tcattaatta cgtgataagc ggataaagga gctgactgat ccatgcgcat
    14581 atccaacggt gcatcttgat gataactaaa tccacgttct gcttcatcta gctgaggaga
    14641 ggaaacccct agatcataca aaatgccgtc taccttatct acatgctcac tcaagcgttc
    14701 ttctaattct ctgaagttcg ctttgataaa tgtgaccata ccttgttcga cataagaagc
    14761 taaacgcagc ttggcgtgat cgattgcttt ttgatcttgg tcaaacgcat acagatgccc
    14821 tttagaactt agttggctta ataaatattc actatgtccg gcaccgccga gtgtacagtc
    14881 tacataaacg ccatccggtt tgatgttcaa tccatctacc gtttctttca acatgactgt
    14941 atagtgttga aatgtttcag tcattagtca gcctcttcct attcttatag tccgaaatca
    15001 atcatacttt cagcaatttc gtcaaagttc tcttctgctg ctgcagaaaa atcttgccat
    15061 cgtgtctcat cccaaatttc aattcggtta gatacaccga tgactacgca accttttgtc
    15121 aaagctgcat aatttcttaa cgtgctgggt atattgattc gtccttgttt atcaagttcg
    15181 cattcagtgg cagccgaata gaagaatcgc acaaaagtac gtgcgtcttt tttagccaac
    15241 ggcatttcgt tcagtttagc ttccagattt tcccattcgc tcatcggata gccgaaaaga
    15301 cagccatcca atccgcgtgt cagaacaaac ttttcgccaa gttcttcacg aagttttgag
    15361 ggcacgatta aacgtccttt tgcatcaata ttatgttgga attcacccat caacattgaa
    15421 agccccccat tcctccacta actaaaatta gtctaccaca atcccccact ttctaccacc
    15481 ttttctggga aatccgggaa caactttttt tattcataaa cattttttta aaaatcaagc
    15541 tgcaaaaagc cttttaaaag actttttaca gcttttctat ttttttcata ggtaagaaag
    15601 tatgttcaca atgatcaaga cagcgtacaa gacgattgtc aacagaaaaa ccaaacgcca
    15661 agtcatcttg aaataacgcg gataaataat ttcttcataa taatatgctt ggaaaacagc
    15721 aatacaaatt cccattagca aaacagaaat aaagaaaaac ggtaagatag aaattgtccc
    15781 tgtatttcta gacaatgcat gcataccgcc ccacaaaaaa ggaatcgcca aatcaggggc
    15841 ttttatttta aaacgacgat ttaacgaaaa taattttaca agaaattggc aaccaaacaa
    15901 aacgattacc gggtaaatat accaaaacaa gataagtggt gtgaatgtgc ccatgaacgt
    15961 aaactccttt tttatcgttg cttcaagtat actgtatata ctttacacaa ctgtttcatt
    16021 ttatttaaat ttatgcagtt atattctata tcttacaaac acattccatg tattccaatt
    16081 ccctatccgt tataataaaa gaaacaggca acaggaggaa aatcatgatc aaatatttta
    16141 cattagataa tgagaaactg cgtaataccg gaaaggaaac tgaccaaacg atctggtatt
    16201 gtgtagaacg tcctaatcaa gaagagattc atcagctgac acatcaattt gaaattccgt
    16261 cagattacat cacttccata ttggacgatg cagaaaattc ccgtacagaa gaattcaatc
    16321 aatcaaagtt gacaaagcca gcgttattat tgttacaata tccttttgct aaaacaagtc
    16381 ctagcggtta ttttcaagtg gatacatacc cgctctctat tattttgacg cccaaaaaga
    16441 agcttatcac aattacgaat catgaaccac tgtttttaaa gaaagtcttt agccagacct
    16501 ttccaaaaag tgatatttcg cctaatctca atatcttctt tcaattcatt tggcaggtca
    16561 cactctctta caacagttat ttagctacgc ttcaatctca atgcgatact ttggaagcca
    16621 aacttcaagt atccactgaa aacaaacaac tttatcaaat catggatatc caaaaaagcc
    16681 tcgtttattt cagagaagcg acttctgcta atttagatgc attgaaagaa ctagcgcaaa
    16741 ataaactttt ccaagaaaat catgctttga aaaatcattt acgtgatgta ttggtggaaa
    16801 cagagcaggc aatgactact tctcgcattc aattaaaact agtagaacaa atgaatcaga
    16861 ctttttcagc aatcgtatcg aataatctga ataatattat gaaaatttta acttctttaa
    16921 cgattatttt aactataccg acaatcgtcg gcagtatcta tggaatgaac attaagttac
    16981 ctattgctga aagggatgat gcctttatat ggctagttct attaacatta tttatttgta
    17041 tcgtaactac ctattattta aaaaaaggga agtttttata aaaaagccct cttgtggaaa
    17101 tggaaaaaag attgtacact tgaatcaact aattaatgac gaggttgaaa acaatgcgtg
    17161 aaaaaaaaca aacaagtact tttgctaaag taacaaaatt tgtcatttgg acaatgctga
    17221 ttttaacgat tggatctgtt gtattgactg ctgtaatgag tgtcatgtaa aaataaaata
    17281 tagctatcag ctgctgaaaa ggacctgaac cactgcgagg tggctcaggt cctttttgcg
    17341 agtttcaata gttcttttgc atgttcgagt gtgagttttg taatctcact tccagccagc
    17401 atacgtgcaa tttcttcttc tctttctttt gttttcaact ctgaaacaga agtttgcgta
    17461 cgtccatcaa tcactgcttt cacaatataa tattgatagt cggcgactgc agcaacttga
    17521 ggcagatgag tgatgcacaa aacttgagag tatttcgata tttgagaaat tttatctgcg
    17581 atggcctgtg caactcttcc gctcacacct gtatccactt catcaaaaat aatacttgtc
    17641 acaccttgct cagaagaaaa aatagttttc aaagcaagca acatccgaga cagttcgcca
    17701 ccagaagcga ctttcaccaa aggcttcagt ggctctcctg gattggtcgt aatgtaaaat
    17761 tcgatttggt caaatccatc atgattcaat tgtctatttt cttcttttaa gaaacgtacc
    17821 tcaaactcag tgttttccat atacagactt ttcaattcat gcagaatgga tttttccaat
    17881 tcttttgcga tttttttacg cgcttcatga agatctgcgg cttgttgctg aagtagaagc
    17941 tctttttgct ctagttcctc tttcattttg tccaattgac cttctgaaaa atcagaagaa
    18001 gctagctctt cggtgatttc gtcatagtaa gccaatattg actcaatgga ttcgccgtat
    18061 ttacgtttca actgtcggat taattctaaa cgttgcgtaa cttcttctaa acgactctca
    18121 tctaactcca ataaatcaat ctgacgactc atatcgccga ttgcatcttg cagcaaatag
    18181 taagcacttt ggatattttc ataaattgct tcatactcca catctagatg ggcgatccct
    18241 tgcagttctg aaacggccaa gccaacacca tctacactgt tttgttcatt attcgtcagt
    18301 gctccgtaac ctgcagctaa tccatcagca attttttgat aattgctaag tttttctctt
    18361 tcttctttta atttctcttc ttctccgact tgcaaatcag cagcagctat ttcttcttgc
    18421 tggaaatgga gcatatctat tctctgaaca taggattttt cattttgctg cacttttcgt
    18481 accctttttt ccagctcacg ataagagata tattcttgct gataactctt tttcttctgt
    18541 tggaaagcta catctccaaa acgatccaat aaagcaagat gtgattcagg ctggagcagt
    18601 tcctgatgtt cgttttgtcc ttggatatct actaaataac tgccgattct tctcagatta
    18661 gctaatgtga taatatgacc attcactcga caaacatttt ttccagttaa ggacatatcc
    18721 cgtcgaacaa tcagattgtc ctcatctgtt tcgatcccta attcaaccat cagttcagag
    18781 aatccttctt gttttggtag ttcaaacaac ccttcaagca cacatttttc tgctccttga
    18841 cgtatataat cgcttgaacc tcttcctcct gctaacagac ctaaagcatc gataataatg
    18901 gatttacccg caccagtctc tccggtcagc gcggtcattc cttcgtgaaa agaaagccgc
    18961 aattccggaa taatcgcaaa gttagtaata ctgatttctt gtagcatcac attcgctcct
    19021 caacttaaag gtaatgcagg aagtctttct tcaataaaac tgcctggtct tcagttcgtg
    19081 taatcatcaa aacattatcg tcatcattga taatcgaaaa taattctttc ttgtaacgtt
    19141 tatcaatcaa atttgctgct gccgaagcat ttcccggcaa tgttttcaaa ataacaaatt
    19201 tttccatttg gtcaactgcc acgaaagcgt cttttaatag tttttccaat ttagtattta
    19261 catcttcgtt tgtttcagct ggtatgctat aacggtagcc gccatctgaa gccggtacct
    19321 tgatcagttt cagttccttt atatctctgg aaatcgtagc ttgagtaaca gatattcctt
    19381 tgcttttcag gatttcaaca aattcttcct gctttcggat atcttgctca ctcaacaatc
    19441 gagtaatcaa acgatgccga tctttttttc tcattacgtc ttcgctcctt agcatattct
    19501 tcttaatcaa tgatagctta tttccttggt ataataaaga agaatccgca tacttctcac
    19561 ttgattttca cagttacaat ttatttatgg tcaaactgtt cgtgtgcatt tgaaaccact
    19621 tctgccatat taatagctga attgattgtt ccgctttcgg gtactttcct caagtgagcc
    19681 aagaactcga tatttccttc tccccccgtg atcggagaat aacttaattc cagcacatca
    19741 tatccacctt gtgccgcaaa tgacaaaatg tcttctaaaa ctttttgatg ggtctgaggg
    19801 tcacgaacaa ttccgttttt ccctacagct tctctgcctg cttcgaattg gggtttgatt
    19861 aaagcaatca cttctccctc ttcttttaaa atagcatgta atggaggcag catcagtttt
    19921 agtgaaataa aagaaacatc gataacagca acttctggaa ttcctaaggt aaagtcttcg
    19981 ggttttgcat aacgaaaatt cactctttcc attacgacga ctctaggatc ttgtcttatt
    20041 ttccaagcta gctgattgta gccaacgtct aaagcataac tcatttttgc gccattttgc
    20101 aaagctgcat cagtaaatcc gccagtggat gcaccaatat ctaacaatgt ttttccttca
    20161 acagatagat caaatgcttt gagtgctttt tctaatttta atccccctcg agaaacataa
    20221 ggaagttttt tacctttgac tcttaattca ctactcactg gaattttttc tcccggttta
    20281 tcaaaccgct cgtttttttc gttgtaaatc aaaccagcca tcactgaacg tttggcttgt
    20341 tctcttgttt caaataatcc ttgtttgacc gctaatacat ctacgcgttc tttttccatt
    20401 ctttccctca tttctccaca tcaaattgct tgatcatctg gcgcatcagc ccgccatcaa
    20461 aagaaaaaac gtcctcttct aatttgtcta tgatcttgtt ggcggataac agctcaatct
    20521 ccaacgcctc acgtgtcttt tctaggccta ataaacgcgg gtaagtattt ttttccatac
    20581 gttcatcttt accagctgtt ttccctaatg ttttcgttgt accaatcaca tcgagtagat
    20641 catctcttat ctgaaaagct aaacctaaat gttcggctaa tcgttgaagt agcaataaga
    20701 tttcttcagg ttgcttcgct aatatgccac cagccagcaa ggcataacga atcaaatgcc
    20761 ctgtctttct ttcatgaata aatgctaatt cttctaaaga aagctttttc gattcgccct
    20821 cgatatctgc agcttgccca gcaaccatac cttgtgatcc cgcacaaaca gctaattgct
    20881 gaagcagcag aagcttagga gaattaccta agtgtgccat actgattagc tggaatgctc
    20941 ctgtaagcaa accatctccg gccaatatag ctaatgcttc gccgaatact ttatgattgg
    21001 taggttttcc tctacgaaga tcatcattgt ccatcgcagg caaatcatcg tggatcaagg
    21061 aatacgtatg caccatttct aaagcagctg ctacttgata gacgggaaca tcaataggct
    21121 catcaaagga agcaacggcc gttaaaagta ataagggacg tattcttttc ccgcctgctt
    21181 tcactgaata actcatcgca tcatagagtc ctctatccgt tgtatgctca tctaaaaaag
    21241 acaagatttc tttttctaca gcaggtagat gaatagctga aaagtcagtt aatctcatct
    21301 cagctttcct cgtttccgtc aaaatcaact tcttcatttg attctgtcat cattttcgtc
    21361 aaggtctctt ctgctttagc taatgtatct tggcaatgtt tgcttaattc cattcctcgt
    21421 ttaaacgaat ctagagctga ttctaatgga acgtctcctt gttctaactg catgacaatt
    21481 ttttctaatt cttgcagtga ctcttcaaaa gttggatttt ccatattttt attctccttt
    21541 gtctatctct tgtacttgtc ctttaacgat tccatcttga taatggatcg tcagcaaatc
    21601 cccttgatga atctcggtga cactttttac tacttgatca tttaacgtcg tatagctgta
    21661 gcctcttccc atgattttca atggactcaa caaatcaaga gattgaacag cttgagcaaa
    21721 acgctgctgc tgttgtttca tatattgttg gattttctct tctaagcgtt tttctagata
    21781 agcagtttct tggcgagctg ctttaactcg ataaagcgga gccacttgtt ccaattggtg
    21841 gcttaatgcg atcgtttgtt tttctttcgc atgataaatt tgctgtgtag cttgatataa
    21901 tcgctgtttc aactggtcta atttgatggt ctgaccttca tacaatcttt caggctgccg
    21961 aaatacatag gaacgtcgag cacgctcgaa ccgttcttgt ttgcgctgga tctggcgtac
    22021 aaacgcctgc tgcaatctag cttgccgttc ttgaatgcgc atcaactctt ctgacaaaac
    22081 aggaacagct aattctgcag ctgctgttgg agtggcagcc cgaacatctg ctactaaatc
    22141 agcgatcgtg gtatctgttt catgtcctac agatgaaatc accggcgtat tagcttgata
    22201 aatcgcccga gcaacccgct cttcattaaa cggccataaa tcctcaatcg aaccaccccc
    22261 acgaccgatg atcattgtat cgaaattgcc catctcttct actctctgga tattcctgac
    22321 aacattgtcc gctgcttggt ttccttgtac aactgttggg aacaaaacca attggacaat
    22381 tggatatctt cttttcaccg tcgtgatgat atcgcggata accgctccgc ttggactggt
    22441 aaggaccgcg attcgctttg ggaaacgtgg cagcattttt ttaggccctt gaaaaaggcc
    22501 ttccttcatc agcttttctt ttcgttcttc caattcttga tataaagcac ctattccatc
    22561 aggctccatg tgctctacat agatttgata agaacctccc gcttcatata atgaaatacg
    22621 cccaacgaca aggactttca ttccttcttt tggctggaat cgtaattttt ggaatgctcc
    22681 tttgaacata atagcagata tttttgcatg gtcatctttt aagctgaaat attgatgggc
    22741 attggcacgt aaacggaaat tcgatatttc gcctgttaaa tatacgcgtt ctaaataagg
    22801 atcggcatca aattttcttt tcaaatactt cgttaatgta gtgacagtta aatattcttg
    22861 tgccatctat tcacctgctc caaaagcttc ggcagatttt acggtttgat acatcagcat
    22921 cgtgatagtc attggcccta caccctttgg cacaggagtg atccagcctg caataggctc
    22981 cacttcatca aatttcacat caccgatcag cttaccgtta gcatcacgat tcatccctac
    23041 atctatcact acagcacctg gttttacgaa ttcttttgtg acaaaatgtc cacgtcctat
    23101 tgctacgact aaaatatctg cttcttgtgc cagttcagcc agattttcgg ttttagaatg
    23161 agcaacagta acagttgcat ctgccatcaa gagtagctga gccatcggtt taccgacgat
    23221 attgcttcgt ccgataacta ctgctcgttt acccgccgga tcgatagaat aagcttcaaa
    23281 cattttcatg atgccgtatg gagtacaagg aatcttgtcc ggtttacccg caaggagtct
    23341 gcctaaattc atcggatgaa aaccgtctac gtctttttca gggcggatag ctaacaggat
    23401 cttttcttca tctatatgct ttggcaatgg taattggacc aatataccat gaaaactagg
    23461 atcttgattg tatttttcaa tttctgcaag tagttcgtct tcagaaatcg tctcaggata
    23521 acgctctacc aatgaatgga ttccgatttc ttttgctaca cgttctttat ttcttacata
    23581 gatttggctg gctggatttt cccctaccaa taaaacgact aatccgggat ggataccatt
    23641 atctttcaat ttttggattt tttcagcaat ctctgcctgc attttttcag ctaattcttt
    23701 tccgttaatc aattctgcca tctaaccact cccagatatt ttttgtgttt tcattctacc
    23761 atagaatttt cccgatttat aacaggttta tgatgattca tagaaaaaag ctaggactta
    23821 cgccccagct tctaattctt tcaatacatt ggataaaaca ccgttgacga atttacgtga
    23881 acggtcatca ctaaatgttt ttgccaactc gattgcttca tttaaagcaa cagccgctgg
    23941 tacatctgtc acataagtca tttcaaaaat cgcgatacgc aagatcacca aatccatttt
    24001 tgagatccga ttgatgtgcc aattgttttt taaatgtttt tcaatcactt tatccaattc
    24061 atcttttttt gcacagacac cgccaactag cgtatcaaga taaacaggaa caaactctga
    24121 ttcatcttcg ttgatcatgt cgcggtggtc taattcgatt gcgttgaaaa tcgcatcttc
    24181 ttttgttagg tctgcattga agtctaatgg aaataaagcc tgcaatgcca tttcccgaat
    24241 ctcgtgtctt gataattctt tattcattct cttcctcttc gtcttcaaaa agttcttcga
    24301 ttgttggttg ttctaatttt tcaggaacaa cagctactac atggatattg acttcagata
    24361 aatcgatatc cgtcatgaaa aggacttgct ggctgacccg ttcctgcatt tccattgcta
    24421 ctttaggaac tgaaacacca tattctagat aacagtaaag atctactttc aagccatctt
    24481 cctcgtttgc taagtaaaca ccttttccgt gagctgcacg tcctaataat tcagtgacat
    24541 tgctggcaaa agttccgcgc attccgtata cgccttctac tttagaagct gcaatcccga
    24601 taattacttc gatgacttca ggagcgatca cgatctctcc taaatcttgg tttgcgttta
    24661 aaatcaaatt tttttcatct gccatttatt tattcctccc tttgcctaag cacgagatac
    24721 atccgaattc tctatgatgc ggcatagtga tcacgtggta agaaaactgc cgccgatcag
    24781 ctgcaaactg ctatgaattg gagaaaacac ccaactttat tatctcttac agcttaaaag
    24841 ttcttctata gtgtatcatt aaatcgagtg attttctata acaaagtctg tgaacactga
    24901 tttttacgct tacttcttct attgatgaaa ctataagatg atcaattctt ttggtgaacg
    24961 agtcaacaca cgatttccat cttctgtgat caacaagtca tcctcaatac gtactccacc
    25021 gattcctggt aagtagatcc ctggttcatc agtgatcaca tttcctggta caaattgctt
    25081 ttctgcacga gaagaaacgt ttggtccttc gtggatctct aaaccgatcc catgtcctgt
    25141 agaatgtcca aatgcttccc cgtaaccatg ttttgaaata taatctctag caactgcatc
    25201 taattggaca ccagtcattc ctggttttgc agcatcaata actgctaatt gagcatctaa
    25261 gactatttga taaatttctt ttaatttttc cccaggatca ccaatagcga atgtacgtgt
    25321 catatcggaa acgtaccctt cgtaatagca accaaagtcc aatgtgatca agtcaccttg
    25381 ttcgatgatt tttttgcttg ctacaccatg cggcatagcc gaacgcaatc cgctagcaac
    25441 gatcgtatca aaagaaacac ctgaagcgcc aagagaacgc atataaaagt ctagttgatt
    25501 tgcgacttca atctcagtca tgcccggttg gatcattttc aggatgtgat catatgccat
    25561 atccgcaata tgacatgctt tttcgataat agcgatttct tcttcatctt tcacttctct
    25621 gagttcttcg atcatctgtg aaacaggtac taattccgca tcgatgattt cttctaaaac
    25681 cgaatattct aaaaatgaga ctgttgtttc ctcaaaagct aatgagtgca gttcttcttt
    25741 gcgtactaag tttgctacct catcaaaaat aggtccgaca tttttaataa tttcaaaccc
    25801 ttgagcctgc gctgccgcct gctcggtata acgaaaatca gtaataaaaa atgctttttc
    25861 caaagtaata acagctaatc ctgtcgtacc tgtaaagttc gttaagtaac gaagattgta
    25921 cggactggta accaaaaatg aatccaaatt ttcttcttgc atttttttgc gcaatttttc
    25981 tacacgtagc atcataatga aactccctct ttctttgcac gcttttttcc attatagcaa
    26041 acttttcttc gtacacgaca gttaaataaa aatttccgga aaacttcttt ttattttaaa
    26101 taaataaagc caaaaaggag tcacagtctc gctgcaactc cttttagttt actattttgt
    26161 aaaaacttat gatcgttttc caaagaaaaa tgagacaact gctaccaaaa tcactgcgcc
    26221 taaaatagat ggaataagtg ccatacctgc agcttttggt ccccattcgc cgaaaatcgc
    26281 ttgtccaata cttgaaccga ctagtccagc aatgatattc gttacacagc ccatcgaatt
    26341 tcctttactg gtgatcgcac ctgcaattgc tccgatcacg gctccaacaa tcaatgtcca
    26401 taaccaactc ataatataac ctccttcttt gctaatcaat tttagcatat gggatttaaa
    26461 ggggacaaat aattggtttt gctatttcat attttcttct gcaaagtaaa aaaataaaga
    26521 aaacctcctc ttagaagttt tctttatttt ttacttgcgt ttaaacatac tacgaatacc
    26581 acgtttcact acatcaaaga aactcaatga ttgtaccatt ctatcttcat ctacatattc
    26641 tctgatcgca cgaagcactt ttttaggttc tttggaagaa aatgtgtatg taccattttt
    26701 tttcgtttga atggcatatc gaggaatcca ttttcccttt agcatgactg atgctagtac
    26761 ataatccact tcttcccatg gaatttggat atatttgcgt ctatctcgcg catggtaaaa
    26821 ctcaaaacca ttatcaccta tcatgatttg cccgtaatct gtcagacccg taaacgaagt
    26881 agcatcaatt accaagtcta cttttgtatt gattgattga accattttct ccctccagat
    26941 atttgtttat gaacagtata ctatataaaa aaagctcggg acaacgcccg agctatattt
    27001 ttatggatta taaaagtccg atgacgtgtc caacaacacc gactacgaat aaaccaagga
    27061 tgataacgat tggagaaacc tttttcttaa gcaaccacat acataacaat gttaatccta
    27121 atgcagctaa accaggaatc aattggtcta agttgtcttg taatgttgtt accttttcag
    27181 gagaaagggc taagccatta ttaacttgtt caaatgcact cttgattcct tcaccacctg
    27241 aaggcaattt atcccattcg atgtaagcac ctttatctaa ttttacagtt gatacaactg
    27301 gtaagaattt gattgatacc catctttgta caagggcagc taatacgaac atccctaaaa
    27361 ttgaagcacc ttttgttacg tcttgtaaca atccgccaga aaggtcatca gtaattttag
    27421 aaccagcttt gtagccaaat tcttgtgtat accacatgaa tgcccaacga ataatgttcc
    27481 aacctaagaa gaagatgatt ggtccaagga tatttcctcc catagctaat gaagcaccta
    27541 gtgcacctag cattggacgt actgtaaacc agaacactgg gtcaccaaca ccagctaaag
    27601 gacccatcat acctacttta accccttgaa tcgctacatc atcaacaggc gcgccattcg
    27661 cacgttcttc ttccagagcc aatgtaaccc ctaaaattgg agaagcaatg tatgggtgtg
    27721 tattaaagaa ttccaagtga cgttttaatg ctgcagaacg ttcttctttt gttttgtata
    27781 attttttgat tgctggaatc atagaaaatg cccagccacc gttttgcata cgttcgtagt
    27841 tccatgaacc ttgaatgaag gtagagcgcc acgcaacagc taaacgatct ttttttgata
    27901 atttgatttt ttcttctgcc attttatttt cctccttcaa gattaatagt cattcaaaat
    27961 gtcgcctaaa gggtcaccag agttgcttcc gcctccatta tttgatgaac cacccatttt
    28021 agaaaggttc aagtaaataa gagctaatgc aacacctaaa gcacctagag caatcaatgt
    28081 taattgagaa attgcagcta caacgaaacc aataacgaag aatggccata cttcttttgt
    28141 agccatcatg ttgattacca aagcgtaacc tacagctacg accattcccc caccgatagc
    28201 cataccatct gttaaccatg ctggcattga ttcaaggaat gattgcacag tttgtgctgg
    28261 gataaatagc aacgctgctg ctgggaccgc gatacgaacc ccttgtaaac atacagcaat
    28321 gatgtgccac atttcaacct tacggatgtt tccttcttca gcagccgcat ccatcaaatg
    28381 aacgattgga acagcgattg tacgtacgat cattgttaag aacaaccctg caacagccaa
    28441 aggaacggca atcgcgatcg cagaaggaac acctttaaca ccttgtccac ctaaaactaa
    28501 aataattgct gatgctactg atgctaatgc agcatctggt gctacagcgg caccgatatt
    28561 tgcccaacct aaagcgatca tctgaagcgt accgccaagt acgataccag cttcgaggtt
    28621 accggtaact aaaccgatca atgtacatgc cactagtggt tgatgaaatt gaaattcatc
    28681 aagaatacct tccataccag ctagaaaggc aactaatacg actaaaatta ttgaaataat
    28741 agacatgata agcctcctat aaattttaat attatttatt acgcttttgc taattcagct
    28801 tttgcttttt tcaaaatatc atccatgtta tcgcgtgaat cattaggtac tttacgtaca
    28861 tcgaatttca cacctttttg ttcaagcttc tcaaacgctt caacgtcttc ttgtcccata
    28921 gacaatactt tacttacaac gactttaccg accgaatgcg ccattgaccc aacatttaat
    28981 tctttgatgt ctacgccacc ttcaacagct gtcaatacgt cttcaggatt ttcaaataaa
    29041 agcaatgctt tggtatttcc aaaacgaggg tcttttgcta cttcgatcat tttgctaata
    29101 ggaataacat ttgcttttac acctggagga gcagcttgtt cgattagtct cttacgcaga
    29161 tcatcctttg atactgcatc tgaaacaacg ataatacggt ttggttgcac tgcttttgtc
    29221 caagctgtcg caacttgtcc atgtaacaga cgtgagtcaa cacgtgctaa tacatatttg
    29281 atttttccgt ctccgaccac agttccttct ggaagtgctc ctttaggttg tgcgtcttca
    29341 actgctgctt tcggtgcttc tgccggttct aattcttccg gttttacttt tacaccgtct
    29401 tttgctgttt caatgatgtg tgctgcaatt tcatgagcag attccattga aaagcgtgat
    29461 gcgtaggctt caatcaacat tggtaaattc aaaccgctga cgattgccca tttatctttg
    29521 tgctcttcga ataatgtgtt tgattgattg aacggtgttc cgccccataa atcaactaag
    29581 aataacactt cgtcttgatt atcaaaagaa gcaaccgctt cttccaattt ggctctcaag
    29641 tcatctggac cttcacttgg tttcaaaatc acggctttga cgttttcttg ttcgccaaag
    29701 atcattgaac cggattgtaa gattccttca gcaaattgtc catgacttgc taggataatc
    29761 cctaccatat ttatacctcc tatttacttt tgttgtaaca aattcaaaaa tattaataat
    29821 tgctaaccat tacaacagaa ttatttgacc ttttccaaca accattcttg ctacaccttt
    29881 ttgattttcg ctagtagttc catcaaaagg attttcctgt cagcaggtac ttttcgaatc
    29941 tctaactcca cttcttgact gttcaagtaa cgaaaggaat caatatcttg ttgatttact
    30001 gaaacaaaat tggtaatcat tgatttgcct tcagtaaaac tcataccgcc gatattcaac
    30061 gacttcaact gtacaccgca gcggacaacc gcttcaacat cttggggatt ggtgaataaa
    30121 agcatcactt ttgctcgtga taataatcca ctatgataga tttgaatcat tttctgcacg
    30181 gttattacat ttgctgtgat ccccggcggt gcagcttctt ttagtagaaa ctttctcaat
    30241 ttatcttgag cgactacatc gctgatcaca atgatacgtt caacacctgt ttgcttcgac
    30301 caaactgttg cgacttgccc gtggattaaa cgatcatcta tacgtgccaa tcgaatatcc
    30361 atctgcaaaa ctccttcttc ctcactactt cacagaaacc cctatcattt ctgtgtgaac
    30421 aaccacctcc tagcacacac tactataagc aaaaagcgtg ccaacttttt tacgatacac
    30481 taattgaatt taaaggattc ttaaccaata cacaaaaacg atacactata aaagtgtatc
    30541 gtttttgtgt attcatggca cagtgtattc ttttttatct aactgattat ggactagacg
    30601 tagcagataa tacatttctt ctatcggcac ttttactttt atctgatctt ccaacagaac
    30661 aactgaggct gccaattggc tataaagagg atcattcgtt gtatttgtca attcgttttc
    30721 ttccacagtc aatggttcat tccgaataat acgttcgatc attcctgctg tgtgcatcac
    30781 taggttgatt ttgaatccat attcttcatc gccgattccc gtcgtctgag agatttgtgt
    30841 agaaaattgc cacattggat cgattaactt actaccatta ataaagatga agttatcaga
    30901 gataaactca acacacgtat ttttagctga ctcttcatct agggagatga attcagtttc
    30961 ctccaattct gattccatca gcaactgatc caaaatgact tcgatatttt gatcaataaa
    31021 acgttctaaa ggaataaatg gggcttggat attcaaatca gtaatcccag tagtagctat
    31081 gatctgatat tctttttgga taactggcag ttcttctttc acttccacaa tcgacaaagc
    31141 tagaacttct aattctgttt cttgacgctt gtttaccgct cgttcgatca attctttgat
    31201 tctttgtgct gtaccttcgc cagatgcgca gatagccaaa acagcttttt tcttatgaat
    31261 aggttttgct tcctgaacag attctacaga agatatttct gcgtaacctc gaaaattttt
    31321 caatgattca tgcaacatat ctagatctgt ccctaatact gagacctttc gtacagtttc
    31381 caaaactaga gaagttgtta ccatatcaac agtacgtact ggaattccag tgtctctttg
    31441 aatttgcgaa ttaaacgacg caagggaccc catatctact aagagtatga ccccgcttcc
    31501 ttcatctact tcttgtacat tgcgttcaat tcggtccaat gctgttttcg gatccatatc
    31561 caaaggcatg tctactgctc gtacgttctc tgcatctaac aactgttgga caacttggac
    31621 catactgctt gctgtgctgt ttccgtgagc tgcaacgact acaccgattc tgccgcttgc
    31681 ttgatttgcc cgtaacgaga cgagtagaac ggtcaagtaa tagtcttcac tttctggaat
    31741 ctccacttga aaatacgttt cgatataact acgcacttct ttcgcaacag catattcatc
    31801 tggaaaatca aaagccatct cacggatgtt atcattcgta tgccgttctt ctcctaagtt
    31861 gatttttttc aaaaaagaac tgatatgcaa gctcatagca taaataaagt tttgctgaaa
    31921 ctcatatggt aaagatcttt tgaccattac ataaatctga ttggtcactt ctatgatttt
    31981 cggatctaca aattctgcta atttgctgtc tgcattaaaa gaaaaaccat ggtctttata
    32041 gaaagacttc agatggatgt tgatatctgt agaaatgaaa tgattgattg cttcttgatc
    32101 caaaccatcc gacttcagta aagctgcttt atctccgatg atatcatata gattataagg
    32161 taactcatag gagtccgatt ggattttcat gatcgtatca tttggtgcta cagttatctt
    32221 tggctccaat attttagaaa gctctgccat tgcactgcgg ttgcttgcta attgaatcaa
    32281 tccgctacga atcccttctg tcaaatcatc gattgtgatc gagatttcgg gtgattgcat
    32341 atgatttaga aatcctcgtg cacatactaa ctggatattt gacttcaatt gtcctatatt
    32401 tccataggtc acactgccga tcaatgcttt tgctacatct tctgtcaaag aaatctttcg
    32461 ctgcgtgcgg tttgcttcgt gggcaatcat gatttttact aaatcgactt tctcatttgc
    32521 aggacgtttt tcaaaagccg gaagtttgat attgattggg atacgccgaa caaaagtttc
    32581 taatagagaa gaacctgggt cttctgtggt cgcacctacg atccgtacat ttgcttcatg
    32641 ggatttcgtt gtttccccaa gacgactgta agttccatga tccataaagt aaaagatcat
    32701 ttcttgacct tcaggcggca accggtgaat ctcatccaaa aacagcatac cgccatccgc
    32761 ttgatcgata atccccattt tttcttcatc tgcgcctgta aaagcacctt ttgcgtaccc
    32821 aaataaatga ctcatcaaaa gctcagggtt attggcataa tcagcacaat tgaaaacaat
    32881 cagttccgtt tcctcatcta ttacatgatg cgcttttgca aaatgataca tcgcgtgtgc
    32941 aaaataagtc tttcctgatc cggtaggtcc tgtaatcaaa caatttaatc ctcttggcgg
    33001 atacaaaatc gcagcttttg cctgttccac tgaatttttc atgctgccgt tagctccaat
    33061 gatttttgca aagatatcct tagtatctat ttggagtgta tttttttctt cagagaccgg
    33121 cgtttctttg taactgacta catgttttgt aactggcttt acgtgcgtaa cgagtgtttg
    33181 atatatgtaa cgtactggtc gtccgtctgt ctttatcagt tttccttcgc gcactagctg
    33241 attcagatct ttactgctat ttgttcgctg aatccctaac acttcagcaa tttcctgtgt
    33301 tgttactcct tgatcatatt ctgcctttgt taaatgagcg gttttttctt tgacatacat
    33361 ataaattcgg tcaatgcgtt ttgccaatgg tatcgctcct tttctataga ctattgtact
    33421 taggcttcaa aacataatac tatgtattta tttctgtttc gtcaactatt tacctgattt
    33481 tgttttgttg ataacacgcg gatagataga aatgttcaat ttaaaagaac gaaaaatccc
    33541 gatacgattt ttataatgga aacaagaaaa agaacactgt ttaccatata attatttcac
    33601 tgatagcaaa caatatagct tccatcaatt gaaaacgata gttatcaaaa catcctgatt
    33661 ccaaattggc aaaaaaatat taagtgtaaa aagacaaagt aaact
//
LOCUS       DACEPW010000009        32627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEPW010000009 DACEPW010000000
VERSION     DACEPW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.009"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(54..788)
                     /locus_tag="IW819_000341"
     CDS             complement(54..788)
                     /locus_tag="IW819_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1243475.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="IW819_000342"
     CDS             1115..1525
                     /locus_tag="IW819_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1243476.1"
                     /translation="MNFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="IW819_000343"
     CDS             1518..2219
                     /locus_tag="IW819_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1243477.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="IW819_000344"
     CDS             2219..3805
                     /locus_tag="IW819_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1243478.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="IW819_000345"
     CDS             3824..4045
                     /locus_tag="IW819_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1243479.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="IW819_000346"
     CDS             4042..4803
                     /locus_tag="IW819_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1243480.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="IW819_000347"
     CDS             5183..5692
                     /locus_tag="IW819_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1243481.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="IW819_000348"
     CDS             complement(5767..6306)
                     /locus_tag="IW819_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1243482.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IW819_000349"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IW819_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1243483.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7373..8170
                     /locus_tag="IW819_000350"
     CDS             7373..8170
                     /locus_tag="IW819_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1243484.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8198..8833
                     /locus_tag="IW819_000351"
     CDS             8198..8833
                     /locus_tag="IW819_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1243485.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="IW819_000352"
     CDS             8853..9626
                     /locus_tag="IW819_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1243486.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="IW819_000353"
     CDS             10034..10831
                     /locus_tag="IW819_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1243487.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="IW819_000354"
     CDS             10809..11222
                     /locus_tag="IW819_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243488.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="IW819_000355"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="IW819_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1243489.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="IW819_000356"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="IW819_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1243490.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="IW819_000357"
     CDS             15999..16559
                     /locus_tag="IW819_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1243491.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IW819_000358"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IW819_000358"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1243492.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="IW819_000359"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="IW819_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1243493.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="IW819_000360"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="IW819_000360"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1243494.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="IW819_000361"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="IW819_000361"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1243495.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="IW819_000362"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="IW819_000362"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1243496.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="IW819_000363"
     CDS             21906..22484
                     /locus_tag="IW819_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1243497.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="IW819_000364"
     CDS             22536..23018
                     /locus_tag="IW819_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1243498.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="IW819_000365"
     CDS             23135..23509
                     /locus_tag="IW819_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243499.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24187..25539)
                     /gene="brnQ"
                     /locus_tag="IW819_000366"
     CDS             complement(24187..25539)
                     /gene="brnQ"
                     /locus_tag="IW819_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1243500.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25686..26276
                     /locus_tag="IW819_000367"
     CDS             25686..26276
                     /locus_tag="IW819_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1243501.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26404..27754)
                     /locus_tag="IW819_000368"
                     /pseudo
     CDS             complement(26404..27754)
                     /locus_tag="IW819_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(27922..28662)
                     /locus_tag="IW819_000369"
     CDS             complement(27922..28662)
                     /locus_tag="IW819_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1243502.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28675..30267)
                     /locus_tag="IW819_000370"
     CDS             complement(28675..30267)
                     /locus_tag="IW819_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1243503.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30835..31131)
                     /locus_tag="IW819_000371"
     CDS             complement(30835..31131)
                     /locus_tag="IW819_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243504.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31307..32032
                     /locus_tag="IW819_000372"
     CDS             31307..32032
                     /locus_tag="IW819_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1243505.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32025..>32627
                     /locus_tag="IW819_000373"
     CDS             32025..>32627
                     /locus_tag="IW819_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1243506.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKI"
BASE COUNT        10575 a         6071 c         6776 g         9205 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaat ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgaccga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaatgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcaatag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga cgaaaataaa ataacaaaca atttgtaaag ttgacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aaatttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcgaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacgatta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcacagcat gacagcattg
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc actagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tttgcgaaaa ctatttttat atcatatata tgtctaaaat ctatattata
    23881 aaagaaatgt tgtttgatca gcttctcttc ggtattagtt caaaacaaaa cggcaaagta
    23941 tatggagcta ctttgccgtt ttgtttctct aatactcagg tcagtttata gacgctcaat
    24001 aaacggtgtt caaaagccag tttctcggta ttaagtcaaa tttatgcaaa aatgtgagaa
    24061 gccattttca aaaaatattt cttaatactc gaaactgagc ggctttttca cgacctctca
    24121 ataaacagtg ttcaagagac agcctatcgg caataaggct gaacgtctct ttcacaacct
    24181 cttttatcaa tctgctactt cttctttctt agcgaaactc caaatcagtc cgataacaaa
    24241 accgataact gctggtacga tccaccccat ccccaatttg aaaaacggca agtagctttc
    24301 cgcaacatat aaaagatttt gaacaatagg tgtttgtttg atagtttctg ggcaagcgtt
    24361 caacccatca aaaatcgaag caatcaatgt aaaataagta gtcattcgat aaacagccgt
    24421 acgctgctta aacaaaggac caactaacac tagtaaaatc agtgtcattg ctaatggata
    24481 gatgaacatc aaaacaggca acgaaaattg aatgatattc gtcaagccta cgtttgccgc
    24541 taaacaagga aggatactag caagtgcaac aaagaatgcg taatttcttt gcggaaacaa
    24601 atctgtgaat gtttctgcaa aagctgtaat caaaccaata ccggtcttca agcaagctaa
    24661 tataacgata aatgctaata agatactgcc ataagtacct aaataatgat cagcaatctg
    24721 agccagcgtg atcccgccat tttcacttag agcaaagcta cctaagctca tagcacctaa
    24781 ataagcgagc aaagtataaa tgattcccat caatataatg ctgatcgcac cagatttgat
    24841 cgtgtcttta gcgatttcag caggttttgt tacacccatg ttttggattg ttgtgacaat
    24901 gatgatccca aatgctaaag cagcaagcgc atctaatgtg ttatatcctt gagtaaaccc
    24961 tgtgaaaaaa gcatgctctt gataatttgg ctgaataggc gcttgattta cagaacctag
    25021 tggattcaaa aaagcaagga gaagcaaagc acctaataaa accaaaaatg caggattcag
    25081 gaactttcca acataatcta atatctttgt cggttttctt gaaaaccacc aagccagcac
    25141 aaaaaataca atactgaaaa ttgctagata tagcgtctgt ctgctaggat cgataaatgg
    25201 tgctaaacca atctcgaaag atgtggttgc aaggcgaggt aaagcaaaaa acggtccgat
    25261 aactaagtaa agcaagatgg taaaaaccat cgcatatgtt tgatttatcc gttttgctaa
    25321 ttcatataca cctgagcttt tcgatacacc aatggcaatc acccctaaga agggaagacc
    25381 gataccggta accaagaaac ctagattcgc ccaaaaggta gcagcgcctg cttcttgacc
    25441 caaatgaacg ggaaagataa gatttccagc accaaaaaat agaccgaaaa gcatggagcc
    25501 tataaaaaga tagtctttaa atgataattt ttttgtcata cataactcct ttacggtttt
    25561 tttaaaagta acaaaatttc gaaaaatatg caacaatttt cagaaaattt cctatataaa
    25621 acgacggtta tttaataacg taagtgataa aataaaagcg ctgaatcatc tgaggaggaa
    25681 tcatcgtgac aaagtcgaga ttagaagcat ttactgatgg agtggtagcc attgttttga
    25741 gagttcttgt attagagatc aagatcccta atacatctag ctttcattct ttatgggtca
    25801 tccgaaatac attattagct tataccatca gctttatatt tgttgcggtg atctgggtga
    25861 accatcatcg tatatttcag atggcagaac gaattaatta ccgagtggtc tggtcgaata
    25921 tctttttgct cttttggctg actttatgtc cttctgttac tagttgggtc gggcgtaacc
    25981 ctgaagcttt ttggcctgag atggcttatg tcttagtata cacgatgtgg agtttttctt
    26041 ttggtatctt gatgaggcaa atcatgaaag caaataaacc agattcccat gtcgtgagag
    26101 ttttgtcaaa ggaccgtcga agtcttatct ccatgatgat taacttatgc ttgattgccg
    26161 gtgtcttttt tgtaccgttg atcgggttat tcggacgttt ttttgtatct ggtatttgga
    26221 ttttttccta taaaaaagca gatgcctatt atcagcggtt gtttccgggg aaatagaaaa
    26281 gaggctggga cagaagcttt taactccaag aaataagccg aaatttcaca aaaatttaag
    26341 aagcaatttt cgtaaattcc ttcttatttc tcgaagctga ccacttctgt cacaactact
    26401 ttcttacttc ttacgttcat atttcatatg atagattagt aatagaagca agaaccacac
    26461 aggtgttagt aataaagcga ttctcgtatc ttttcctagg aacaagacga cacacacaaa
    26521 ggccatgtaa agaaatacca gataattact aaatgggtac aacggcattt taaatgaatg
    26581 agcttttttg ccgaggatac ttttacgata tttcatgtgc gtgtagacaa gcatactcca
    26641 gataaacaag aaacaagtag tggcaatact gcttactagt gtgaatactt cactaggcat
    26701 cacgtaattc aagataacag aaatgaaaat gacgatagta gaaaatgtca aagcattccc
    26761 tggcacatga tgtgttgtta actttttgaa attttttagg ggcactgcct tcttgcgcta
    26821 aagaacgaag catacgtcct gtactatata ttgcactgtt gcaggcagaa gctgctgcac
    26881 ttaatacgac taaattgatc aaggaagcag caatcgtgat cccaatttcc gaaaatactt
    26941 ctacaaatgg actgttatct ggattcaagc tgttccatgg atagattgcc ataatgacaa
    27001 aaagagatcc taagtaaaat aagatgatcc tgataggaat attattgata gcttccggca
    27061 acactttttc aggattttct gtttctccag cgactaatcc aactaattct atcccagcaa
    27121 aagcaaaggt taccatttgg aaagctaaga taaatccttt tgcaccagtt ggaaagaatc
    27181 cgccatgaga ccatagattc gtgatactgg ctgtgccaat atctgtcgta tagtgggtga
    27241 ggatcatata agctcctacg aggataaagg caatgatcgc tacaactttg atcaatgcaa
    27301 accaaaactc taactcacca aacaatgaga cagccaccat attcaagcaa agaagaagac
    27361 ctaatgcaat cagttcagga agccactgag gaacaccagg cgcccagtag cgtacataca
    27421 ttccgatggc tgttaaatcg gccattgcaa tcgataccca gcagaaccaa tacgtccagc
    27481 cagtgatgaa agccgctttt ttgccaagat attctgcaac aaaatccaga aaagaatggt
    27541 tattggtgtt tgataacaga agttcgccta acgcacgcat aatgaaaaaa cagatgcatc
    27601 cagtaataag ataggcgagc aatatcgaag ggccagctag ctggatagac tttccggaac
    27661 ctaaaaacaa ccccgttcca atcgctccgc caatagaaat cagttggaca tgtctatttt
    27721 tcagtccacg ctgcagttct tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt
    27781 ttaattactt ccatattaaa agagttttct gaaaaacaca actaagattc caaaattttc
    27841 agaaatttac cttttatgag taattcataa aaaagaactg cggtaaacac cgcagctctt
    27901 tgtcccaatc agctattgat cctaagcatt taaaatacgt gaaaggaatt cttttgtacg
    27961 ttcttctttc ggatggacaa agatatcttc tggtgtacct tcttctgcaa tcacaccttt
    28021 atccataaaa atgacacggt cagatacatc ttttgcaaat tccatctcat gcgtcacgat
    28081 aagcattgtc aagccagtat gtgctaagtc tttgatcgta ttaagcactt cgccaaccat
    28141 ttcagggtct aacgccgaag taggttcgtc gaatagcaat acatctggat tcattgataa
    28201 tgcccgagca attgcgacac gctgtttctg tccaccagat aattgtgctg gtttggcttc
    28261 gatataacga tccataccaa ctttttccag attttcgatt gctacttttt tggcagtttc
    28321 tttatctcgt ttcaggacag ttgtttgacc agacatacag ttttccaaga cattcatatt
    28381 gttgaacagg ttgaaggatt ggaaaaccat acctagattc gttcgatatt taggtaaatt
    28441 atatttagga gctaatacat tttctccatt ataaatgatc tcgccgcctg ttggtttttc
    28501 cagtaaattg atacagcgca agaaagtaga tttaccagaa ccggaagaac cgatgatcgt
    28561 cacaacttca cctttattga ctgttacgct gatatctttc aatacttcat tttcaccgaa
    28621 gctttttctt agatgtttga tttcaataat tttttcattc atgattcttc ctccttattc
    28681 ctgcaaattt gaattgtcga tttcttcaag cttggtataa gctgaaggac catcgatttt
    28741 cttttctacc gcccgcagga ttctggtgat cgtaaaggtc atgactaagt agatgatccc
    28801 gacgatcgta aatgtttgga agaattggaa attcgcaccg gaagcagagt ttccttggaa
    28861 gaacaaatca gctacgccga ttacacttaa aactgctgta tctttgatat tgataacaaa
    28921 ctcatttcct gttgctggca agatattccg taaaacttgt ggaataacaa cttttctcat
    28981 cgtttggcca tgggtcatac caatggcttg cgcagcttcg aactgccctt catctactgc
    29041 aaaaatcccc ccacgaacga tctctgtcat gtaagctcca gtattgagag agacaataaa
    29101 caaagcggca atcgttcggt tcaatgaaat gccaaaagcg agtgccagac cgtagaagat
    29161 gaccattgct tgtaccatca tcggtgttcc gcggaatact tcgacataga tagaaagaag
    29221 tgcatttccg attttttgga agaagcgagt cagtgcattt cctgattcag gtaccgaacg
    29281 gaagacacca atcagtaatc caagtgtagt accgacaact gtcccgatca atgcgatgaa
    29341 caaggtaagt ccagcacctc gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa
    29401 atcagaaatg atgccaggtt tttcttcact atcagttgca gcaggctgat ctttgactgc
    29461 ttggtccata attttggttc gttcatcgtt agagatgcca gaaagaatct ggtttacttt
    29521 ttggatatcc gggtcgcctt tacgcatacc aacagaaatc tgaacatctt ccgggtttgt
    29581 ctggaagcca tctgcaccgc tgaattcaag catttccaag tctttgttca cactagttgc
    29641 cgttacacct tccggacgtt cgctgacata accatcgatc attccagaag ctaaagctgt
    29701 tctcattgct gagaaattat ccattgcttg ttgtttctgc acatctggta tctgatcgat
    29761 cacagtatag tggaatgtat ttaactgagc agtaatcttt gctccagaaa gatctgctaa
    29821 actttttgct tttgcatatt tgctatcttt ttgaactact actactaatt gtgattcgta
    29881 ataaggatcg gtaaaatcaa tttcttttcg tcgttcagca gtagggctca ttccggcgat
    29941 gatcgcgtcg atcttgcctg attgaagggc aggagccaga ccatcccatt tcgtttggac
    30001 gatgactaat tttttcccta aaccatctgc tactttttta gcgatttgaa catcataacc
    30061 gccggcgtaa ctattttggc cttgaattgg caaggcatta ttctcatctg tttgttgaga
    30121 ccagttgaag ggtgcatacc cagcctccat acctacgcga aactcatcgc tcggtgtttt
    30181 ttcatctgca tttgccagag atcccacact gaatagtgag aagacaaaag caaatagtac
    30241 ggcaactgaa accatatttt ttttcatttt tttctccctt tctgattata tgatacacga
    30301 gcggtctaga tgaaatgcgg taaagcttta tctgaaaaaa taaaaagcga ccgatttata
    30361 gatataaaaa ggccgctaaa attccataag gtgataaacc tctcacaaaa catagcgcaa
    30421 cttagcatca ctgctaagac agtccgcaag ctcttaactt gcgtcccaac atacactcca
    30481 agcaaaaaag tgcacatttc ggcgatcctc cctattcctg cttctccatg tttgcttcac
    30541 tccacagggt taatgattct cgcgacctct acctcattga ttgaggcatc tcgtattcag
    30601 ttatttatac aaaaaaagtt tacgatactc gagaggcttt gtcaatctat aactgctgtt
    30661 ttttgaaaaa atactgataa atatagaaat ctttactttt accgaaaaaa caatcaaaat
    30721 gtgaatcatc tatccattgc aaacgatagt ataattatac acaaaaatgt atgtaataca
    30781 ataaaaaaag aactagaaca gaaaatttcc tgtgtccagt tccatctcga tcatttaagc
    30841 tgccgctttc tttggctcac gtcctaggat cgcttgcaaa agaacaacga cgccataaat
    30901 ggctaatatt agataaatca aacttgagta ttgggtgatt ccccagcccc atgttcgata
    30961 tacggctaat gtaatggcta aaacaattgc ggcaatcacg ttgaataaag caaaagccca
    31021 aagatttcca ttttctttgc gtacaagata aaaaacagag aaataaacgc ttaacgctaa
    31081 ccaggcgata caagccagaa taacgcccca ataaataaag aatggtaaca tatcgattca
    31141 cctcactttt atacagcagt attgtaacac gttttcattc ttttgtcagt gaacattgtg
    31201 ataaattgtt caaatgccca taacactttt ttattcccat ttttctgact tttcaacaat
    31261 aaatttgttc gagtatgggc tataatggaa agtgaggtga caaaacatga aacaattaac
    31321 agtagacgaa gcagcaaaag agatattagc ttatcagcaa attacatttt tgaccggtgc
    31381 aggggtatcc acaccttctg gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg
    31441 gatggagcag ccggaatact tgctgagtca tcaagcaatg gtgaatgaac cggataaatt
    31501 ctatcaattt gtcaaaaaga tttatcatcc tgaagcaaaa cccaatatca ttcatttgga
    31561 aatggcacat ctagcgaaag aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt
    31621 gcacgcaaag gctaaaagcc cgcaactcgt caattttcat ggaagtttgt atcaatgtca
    31681 ttgtcgaaaa tgtcaacaaa cggtagactg gaaagaatat cttcacagcg acaaacatca
    31741 agcatgcgga ggacaaatac gcccagacat cgtgttatat ggtgaagggt ttcaagatgg
    31801 agtcatggag caggcggctt atgccgtcag ccaagcagag ctgatcgtca ttgttggaac
    31861 gagttttcaa gttcatccgt tttgtgatct ggtacagttt cgccagccgt cagccaaaat
    31921 cttagtaatc aatcaaacgc ctgtttattt agcggagcct tatacgttta ttcaagaaga
    31981 cggagcggtt gttttcaaaa aaatacaaga attaggagca gattatgact gaattcaaaa
    32041 ccgaaaaaga aaaaatgatt gcaggcgagc tttattttgc caatgatcct gagctggttg
    32101 ccgaccgtat gtttgcaaga agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac
    32161 tgcgatccca attgttgaaa gagacttttg gccgtacagg gaaaaaaatc tatatggagc
    32221 cagttatcaa ttttgattac ggatataata tatttgttgg ggagaatttt tatgcaaatt
    32281 tcaattgtac atttttagat gtcagcacga ttgaaatcgg tgataactgc atgttcgctc
    32341 cgaatgtcca gctttacacg gcgacgcatc cactacatcc agtgaaacgt aacagcggtc
    32401 tggagtacgc aaaacccatt aaaatcggta ataatgtttg gttaggcgga ggagttatcg
    32461 tcacgccggg tgtcactcta ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca
    32521 aatcttttcc cgacaatgta gtgattgcag gaaatcctgc tcgtatcatc aagacagtag
    32581 aggaagaact aacagaagag tctcttgaaa cattacgtca taagatc
//
LOCUS       DACEPW010000010        31639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEPW010000010 DACEPW010000000
VERSION     DACEPW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.010"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(55..1257)
                     /locus_tag="IW819_000374"
     CDS             complement(55..1257)
                     /locus_tag="IW819_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1243507.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(1339..2028)
                     /locus_tag="IW819_000375"
     CDS             complement(1339..2028)
                     /locus_tag="IW819_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1243508.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(2134..2502)
                     /gene="rplL"
                     /locus_tag="IW819_000376"
     CDS             complement(2134..2502)
                     /gene="rplL"
                     /locus_tag="IW819_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1243509.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2555..3058)
                     /locus_tag="IW819_000377"
     CDS             complement(2555..3058)
                     /locus_tag="IW819_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1243510.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3468..4157)
                     /gene="rplA"
                     /locus_tag="IW819_000378"
     CDS             complement(3468..4157)
                     /gene="rplA"
                     /locus_tag="IW819_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1243511.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4261..4683)
                     /gene="rplK"
                     /locus_tag="IW819_000379"
     CDS             complement(4261..4683)
                     /gene="rplK"
                     /locus_tag="IW819_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1243512.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            5026..5694
                     /gene="sdaAB"
                     /locus_tag="IW819_000380"
     CDS             5026..5694
                     /gene="sdaAB"
                     /locus_tag="IW819_000380"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1243513.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5707..6579
                     /gene="sdaAA"
                     /locus_tag="IW819_000381"
     CDS             5707..6579
                     /gene="sdaAA"
                     /locus_tag="IW819_000381"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1243514.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6670..7689)
                     /locus_tag="IW819_000382"
     CDS             complement(6670..7689)
                     /locus_tag="IW819_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1243515.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7862..8749)
                     /locus_tag="IW819_000383"
     CDS             complement(7862..8749)
                     /locus_tag="IW819_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1243516.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8826..9371)
                     /gene="nusG"
                     /locus_tag="IW819_000384"
     CDS             complement(8826..9371)
                     /gene="nusG"
                     /locus_tag="IW819_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1243517.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9472..9642)
                     /gene="secE"
                     /locus_tag="IW819_000385"
     CDS             complement(9472..9642)
                     /gene="secE"
                     /locus_tag="IW819_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1243518.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9666..9818)
                     /gene="rpmG"
                     /locus_tag="IW819_000386"
     CDS             complement(9666..9818)
                     /gene="rpmG"
                     /locus_tag="IW819_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1243519.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9980..10624
                     /locus_tag="IW819_000387"
     CDS             9980..10624
                     /locus_tag="IW819_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1243520.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10680..11183)
                     /locus_tag="IW819_000388"
     CDS             complement(10680..11183)
                     /locus_tag="IW819_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1243521.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11196..11242)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11780..12745
                     /locus_tag="IW819_000389"
     CDS             11780..12745
                     /locus_tag="IW819_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1243522.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12844..14016)
                     /locus_tag="IW819_000390"
     CDS             complement(12844..14016)
                     /locus_tag="IW819_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1243523.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(14003..15148)
                     /locus_tag="IW819_000391"
     CDS             complement(14003..15148)
                     /locus_tag="IW819_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1243524.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(15162..16086)
                     /locus_tag="IW819_000392"
                     /pseudo
     CDS             complement(15162..16086)
                     /locus_tag="IW819_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            16203..17282
                     /locus_tag="IW819_000393"
     CDS             16203..17282
                     /locus_tag="IW819_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1243525.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17314..17907
                     /locus_tag="IW819_000394"
     CDS             17314..17907
                     /locus_tag="IW819_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1243526.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(18017..18244)
                     /locus_tag="IW819_000395"
     CDS             complement(18017..18244)
                     /locus_tag="IW819_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1243527.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18347..20935)
                     /locus_tag="IW819_000396"
     CDS             complement(18347..20935)
                     /locus_tag="IW819_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1243528.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20955..21614)
                     /locus_tag="IW819_000397"
     CDS             complement(20955..21614)
                     /locus_tag="IW819_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1243529.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21663..22166)
                     /gene="trmL"
                     /locus_tag="IW819_000398"
     CDS             complement(21663..22166)
                     /gene="trmL"
                     /locus_tag="IW819_000398"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1243530.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22387..23253)
                     /locus_tag="IW819_000399"
     CDS             complement(22387..23253)
                     /locus_tag="IW819_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1243531.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23362..24003
                     /locus_tag="IW819_000400"
     CDS             23362..24003
                     /locus_tag="IW819_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1243532.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(24065..25426)
                     /gene="mgtE"
                     /locus_tag="IW819_000401"
     CDS             complement(24065..25426)
                     /gene="mgtE"
                     /locus_tag="IW819_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1243533.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25444..26343)
                     /locus_tag="IW819_000402"
     CDS             complement(25444..26343)
                     /locus_tag="IW819_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1243534.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26345..27142)
                     /locus_tag="IW819_000403"
     CDS             complement(26345..27142)
                     /locus_tag="IW819_000403"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1243535.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(27120..27824)
                     /locus_tag="IW819_000404"
     CDS             complement(27120..27824)
                     /locus_tag="IW819_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1243536.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27955..28530
                     /locus_tag="IW819_000405"
     CDS             27955..28530
                     /locus_tag="IW819_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1243537.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28648..29325
                     /locus_tag="IW819_000406"
     CDS             28648..29325
                     /locus_tag="IW819_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1243538.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(29404..31212)
                     /gene="pepF"
                     /locus_tag="IW819_000407"
     CDS             complement(29404..31212)
                     /gene="pepF"
                     /locus_tag="IW819_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1243539.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(31388..>31639)
                     /locus_tag="IW819_000408"
     CDS             complement(31388..>31639)
                     /locus_tag="IW819_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1243540.1"
                     /translation="KHLLHLSSWIYQKSRFFFYLQEQVFLYRMLYEEALENQKVPDFN
                     SWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
BASE COUNT         9295 a         6712 c         5640 g         9992 t
ORIGIN      
        1 atttggctat tcttattact tgatgcagaa cagcttttca caacctcttt tcgtctattt
       61 tttattttcg agatttctct ttcctttaaa aacaactgtt tgaaagaaga acgcacatcc
      121 ttgaaaaaca agcccgctaa ccgttacacc taaccagcca tactgctgcc acattgtcgt
      181 ccctagcaat gaaccagccg cacctccaat aaagtacata aacataaata cagtattatt
      241 tcggttgctt gctgcttcac ctagcccttg tactcgtgtt tgattggcga cttgaccaaa
      301 ttgattcccg acgtctaata aaataatagc taaaagcaaa atggccaaat ggcttccgcc
      361 aaccaataaa gcaataaagc ttattgtttg cataacaagt cccatataca cgatttttct
      421 ttcagaataa gaatctgcca gtcgtcccac gataggtgca gcaactgctc cagacaaacc
      481 aaaaatcgct aatatccctg cttcgaatgt tccccaattg taggcgggac tgctgatata
      541 aaaaatcaac gtagaccaaa agatcgaaaa tgttccaaac atgaaaaatc cactgactgc
      601 cgcttctctt aatagacgtt gagaagcaac taaatgcggc aaacttttaa gtgaatcaag
      661 gtatgaaaca gctgtactac tttgctggac agaacgcggc attttccagt aaagcagaag
      721 tgtaagaatt ccgactgcac ataaagcaaa taaatagatc atccgccaag aaaaaagact
      781 ggcaacaata ccagataccg tccgagaaag caaaatcccc acgagtaaac cgctaagcaa
      841 tgtacccatg accttccctc ttgcttttgg tcccgctagt accgcaccgt aaggaatgat
      901 gatttgcggc acgatcgaaa gtaatcctat taaaaaagaa gcgcatgcaa ataacgtaaa
      961 cgaaggcgca aaaaaagcgg ccaacagaga gacggcagat aacgctgcca ttcgaatgat
     1021 caattttgga cgatccacca cgtcccctaa aggaactaaa aacagtaacc ctaacgcata
     1081 gcctaattgg gtcaacatcg tcaatatccc gactgcactt gtgctaacag ataaactagc
     1141 tgctactttt gttccaatcg gctggatgta atacatattg gcaacaacaa cgccacaagt
     1201 gattgataat agtaaaataa gcgaagttgt taaaggtttt ttttcattac tcttcattga
     1261 tttcccccat ttcctatctt agtttacaac ttatggaaaa gattggaaat agaaaaatac
     1321 aactattcgc cacacttatt atttgtgctt ttgttttcct tgttttttct tgcgatcttt
     1381 ataaatgaag acaactaacc ctactagcac agtaacactt gcaaccaacg tgactgcagt
     1441 tttgttatat ccgtgaaaca gagcttctcc accacacgca tacaacacag acgaagggat
     1501 gactccaatc acgatcgaca atatgatttg ctgtagaggc aatttcatcg catctgcagc
     1561 aaaattgacc atagaagatg gaataaccgg tatcatatag cctaacatta cacctatttt
     1621 tggatgtttc atttgtcgga ttgcttgcac ccaatgattc gtttcggttt tcttatctaa
     1681 aaattttaag cgatgcataa ggaaaattgc agtgatgtta ccaagagcat tacccaaaat
     1741 gttgatgatc gcacctgtca gtggtccaaa gctcaaacca gcaatgactc caactactga
     1801 agtaggaata cctggaacag cacacatcac cgctgtcaat acgatcaaga gtatcgatgc
     1861 aaaagtccca tgcgaacgaa tatgtgcgag tagctctgcc ttactcgctc tcggatttaa
     1921 aaataattgg atgtctgtct gatattctaa atacaaccga tagcccacta ccagtatcag
     1981 aatgattccc aacgagatca atatcaatcg gattattttc tttttcatat ttcctcctga
     2041 aaaaaatcta tattcattaa ataatagttt aacataaaaa caaaaaagca ccgaaatcca
     2101 acataggatc tcagtgcttt taaatagtaa aaattattta acagttacag atgcgccaac
     2161 ttcttcaagt ttagctttca attcttcagc ttcttcttta gatacgcctt ctttaactgg
     2221 tgcaggagcg ccatcaacta cagctttagc ttctttcaag cctaaaccag ttgcttcacg
     2281 aactgctttg ataactttaa ctttttggtc tccagctgca gttaattcta cagtgaattc
     2341 tgtttgttct tcagcagcag cagcgccacc agcagcagct acagctacag gagcagcagc
     2401 agatacgcca aattcttctt caatagcttt aactaggtcg ttaagttcta ggatagttgc
     2461 ttcttttaac tcagcaacga tgttttcaat gttcaatgcc attataattt cctccgttta
     2521 attgttcttt tttatttaat gctttatgcg gcaattaagc tgcatctcca tcttctttgt
     2581 ctgccactgc tttgacagcg taagccacgt tgcggactgg cgcttgtagt acagatagca
     2641 acatagatag aagtccttcg cgatttggca attttgccaa tgctgtgatt tcttccaatg
     2701 aagagacttt accttcgatg ataccgcctt tgatttctaa tgcttttgct tctttagcaa
     2761 attcgtccat gattttcgct ggtgcgacta catcttcgtt actgaatgca acagctgtag
     2821 gtccagtgaa tacttcgtcc atgccttcaa ggccagcttt tttagctgca cgtgaaagaa
     2881 tagaattttt gataactttc atttctacgt tagcatcacg aagttgttta cgtaaacgtg
     2941 ttacttcatc aactgttaat ccgcggtagt caacgactac tactgatgca gctgctgtaa
     3001 atttttcgct tacttcatca acgattgctg ctttttttgc gataattgct tcactcattt
     3061 ttgagtttca cctcctgaat tttgatggaa gagttttttg cactgaaaag gcaagtcact
     3121 tttatagtta ctttgctcaa ccagtattat tcatcaccta ttcacttcgt tcatagtgat
     3181 tctactcgct ttgctcgaaa ggaaactcct ttcgctttgc tcaaccagta ttgttcatca
     3241 tctcttcact tcgttcatag tgattcacaa taaaaaactc tatgccaccg cagacataga
     3301 gggactattg aatatattca ataaacgtcc tcggtaggaa attaaggctt gatgccacct
     3361 actgtcttcg gtacaagtta attattaacc tcaactagct tagcatgcta aactagttga
     3421 gtcaagaatt atttttcagt tggatcaatc aacattctaa tatttaatta gaaagaagct
     3481 tggtcaacat ggataccagg tccaaatgtt gttgtgatcg ttacattttt catgtattga
     3541 cctttagctg ctgatggttt agcttttagc aatacatcat ggattgctgc aaagttttca
     3601 actaattttt catcgtcaaa tgaaactttt ccgattggta catggatatt tccagctttg
     3661 tccacacggt aagttacttt accagctttc acttcgttta ctgcttttgt tacgtccatt
     3721 gtaactgtac cagttttagg gtttggcatt aagccttttg gtcctaatac tcgtcctaga
     3781 cgacctacag tagccatcat atcaggtgtt gcaacaacta catcaaagtc aaaccatccg
     3841 ttttggattt tttgaaccat gtcgtcgtca cctacgaaat ctgcaccagc tgcttcagct
     3901 tcttttgctt tgtctccttt agcaaaaact aaaacagttt gtgttttacc agttccgttt
     3961 ggtaatacaa cagcaccacg gatttgttgg tctgcttttt tagggtctac gttcaaacgg
     4021 tatgcaactt caactgttgc atcaaatttt gcaatgtttg tttcttttgc taaagctact
     4081 gcttcggcaa caggataaac tttacttgaa tcaacttttt ttaatgcatc ttgcattttt
     4141 ttgctctttt tagccatttt ggttcctcct tgattgtggt tataacggtt gtacctccca
     4201 cgtgttctat atctttcaat atagagccaa ctgagaagct gctgtcttag ggtgtataga
     4261 ttattctaca gtgatcccca tgcttcgtgc agttccttca accatgcgca ttgctgcttc
     4321 aacgttagct gcgtttaggt cttccatttt taattcagcg atttctctta cttgatcgct
     4381 agaaactttc gcaactttat ttttgtttgg ttcgcctgaa cctttttcga ttttagctgc
     4441 ttttttcaat agaacagcag ctggcggtgt ttttgtaacg aatgtgaatg aacggtcttc
     4501 gtatacagaa ataacaaccg gaatgatcaa acctgcttga tcagctgtac gagcgttgaa
     4561 ttcttttgtg aatcccatga tattgatacc cgcttgacct agtgcaggac ctactggtgg
     4621 agctggagtt gctttacctg caggaatttg caatttaacg attttttcta ctttctttgc
     4681 cacgagacat acctccttaa gtccgtgatg tggttaattg gagatgcata tttctcctcc
     4741 cacgttttat ctgatctgaa aaaatccaga caaataatgt gcaaaacaat gcacactggt
     4801 acagtatagc aaatttattt gttttttcaa gatttttttc tgctattttt acattttgct
     4861 tgaataaacg ctaaatttat tctaaaaaaa caacaatttt tcgttctcat ttcttctttt
     4921 atgttacact tgcgctagca tggttcaaca gatgaaagca aacaataaca cggtttttta
     4981 gacccttgta caaagattcc tacataacaa aatgaggtgg agaaaatgaa agatttaagg
     5041 tatagaagtg tctttgatat tattggacct gtgatgattg gtccaagtag ctctcacact
     5101 gccggagctg ctagaatcgg gaaaatcata cgtagtatat ttggcgaaca gcctgattct
     5161 gtagatattt atctatatga atcatttgca aaaacctatc gggggcacgg aacagatatt
     5221 gcattagttg gcggcctgtt agggatggaa cccgatgatg aacgtttagc tgattctttg
     5281 aaattggcat atgaagccgg tatggaagtc ttgtttgttc ctaagagtga aaaagccgaa
     5341 catccaaact ctgtaaagat gcttctaaaa aaaggaacac gaaagctttc agtcactggc
     5401 atttcgatcg gcggtggcaa tatccagatt tctgaattga atggattcaa aatttcttta
     5461 agcatgggta ctcctacctt gatcgttgtc caccaagatg ttccaggtat gattgctaaa
     5521 gtgactagta tactttctga gaccaatacg aatatcggca ctatgacagt gacacgggaa
     5581 tcaaaaggtg aaaatgccat tatgataatt gaagtagatg atcctcaagt agaagaaacg
     5641 gtaaaaagac tcaaacagct gccaaatatc gacagcgtaa attatttcga ttaaggagct
     5701 aaaaaaatgt ttttatcgat tgaagaatta gtagatcaag caaaagctta tccaagtgta
     5761 gcagaattga tgattgcagt tgaaatggaa atgagtggcc gttcccgtag tcacatcatc
     5821 gagatcatgg aaaaaaacct ctcagtaatg gaacaatcaa tcaacgaggg aaaagaaggt
     5881 gttacctccg tcacaggaat taccggtggt gatgcaaaaa aattagatga ttatttaaat
     5941 gaggcagact ttttaagtgg agaaacaatc ttaaaagctg tacgcaatgc tgtagctgtt
     6001 aatgaagtca atgcaaaaat ggggttgatt tgtgctacac cgactgccgg aagcgcgggt
     6061 gttgttcctg gtgtgctgat ggctgtccga gatcggcttg atttgacaca tgaacagcaa
     6121 ttggaattct tatttaccgc tggcgctttc gggttagtca tcgcaaacaa cgcttctatc
     6181 agtggtgcag aagggggatg tcaagcagaa gttggttctg caagtgcaat ggcagcagca
     6241 gctttggttt gtgcaaaaaa tgggactcct catcaagcag cacaagcagt agccattaca
     6301 ttgaaaaata tgatgggact gatttgcgat cccgttgcag gcttagttga agtcccgtgt
     6361 gtcaaacgta atgctttagg ctcttctcaa gcttttatct ctgctgatat ggcattagcg
     6421 ggtatcgaaa gtgtcattcc gccggatgaa gtcgttgctg caatgtacca agtcggacgt
     6481 caaatgccgt cgattttcaa agaaacagca gaaggtggct tagctgttac gccaactgct
     6541 aaacgacttg cggaagagat tttaaataag agggcgtgat aaagctcctt aatttcgagt
     6601 attaagagat tagttatctt cataacaaaa aacgaagaga ggactaattg cccttctctt
     6661 cgttttttat taatccatca atttcgtatt ttttacttca tcgatcgttt ttgttcctgc
     6721 aagttgcatc gtgatcgaca attctttgtt caggtgttca aaaacagatg ttactccttc
     6781 tgccccacct agattcaacc cataaatcac tggtcggcca atcgcaacta agtcagcacc
     6841 acttgccaga gctttgaaga catgctctcc tcgacggaca ccagagtcga aaatcactgg
     6901 tacacgtttg ttgactactt cggcaatttt tggcaatact tcaaaggacg caggacctcc
     6961 atctagttga cgtccaccat gattggatac ccagatacca tctgcccctg cagagatagc
     7021 gattactgca tcttctggcg attgtattcc ttttacgata actggaagat gggtgatttc
     7081 tttgattgtt ttgatgtcat ctggtgtcaa gccttgtttt gcagctgcat aaatttctgc
     7141 gattcctttt ccttcgccat taccacttgc actttgttcg ctatacgcag ctaagttagg
     7201 cattggtaat ggaaattgga attgattgat gacgtcttct tcacggtaac caccgagcgt
     7261 tgaatctgcc gttaaaatga tagctttagc ccctgcttct actgctttgt tcaaaatgaa
     7321 ctcattgaat ctgtcgtctt tactcatata taattggaaa aactgcggtg cgcctggcgc
     7381 tgcatcggct gcgtctttga ttgtcgtatt tgcataagtg ctgatggaaa agattgatcc
     7441 agcagcagct actcctttag ctgtatcagc ttcccctttt tcatgtgcca atccttgggc
     7501 cgctgatggc gcttggataa tcggtgtatc taattcaatt ccgaaaacag atgtatgcaa
     7561 atcggctgag tcgattccgc gtaatatccg tggcatgatt ttctttgtat taaaagaagt
     7621 cgtattttct ttcatcgtcc actcatcttc agaaccaccg cgaatatagc caaaagctcc
     7681 tgcttccatg cgctctttta cacgaccttc tagttaggca atgtttacaa tatcgattgg
     7741 atgttcttca ttacttgtat gataagtcat agtagatcct tctttcatct tcttgattac
     7801 atataaatca tactaaactt acaaaaagta agcaaacatt tactctcaga tacttttatc
     7861 gctattcttt ccaaataaat cgacttacaa gctgatctac ttcttggttt tcatgaagca
     7921 tactatgaga ggcattttca cctgtcacta ctttttcctc tatttcattt ccgcgttgct
     7981 tcaacaacgc atagaccgct aatgcactgt ttaacggtac tgttccatca ctcaagtctg
     8041 tttcatctag ttgaccagca atcaagaaaa atcgagtggt tataggaata ttaccgatca
     8101 tttgctggta atcttgatag cggttagaaa cgacagcagg cccctttgct aactcatctg
     8161 tcagagactg cgcactatca tcgacaaaat cattaaaagg cgcgccaatt gctgttaatg
     8221 tctgaacttg tggcaatgac gtatcctgtc catacgttcc taaatagcgt agtgcactaa
     8281 ctcctcccat ggaatgcccc actatgttga ctttatctat gccgtagttt tctttcagat
     8341 acaaaagaca agttttgatc cattctgctt gattccactc gttattttta ttatcttcaa
     8401 acaatacttg gaccatcgga ttgtctgctt ttccagacaa atctcctgtt gcttgtattt
     8461 gtccatctgt tcctactgtt aaaaccaatt cttttactgc ttgctgattt ttttccaaac
     8521 gccgcaacat ccctttaaag gaattttctg tcccgctgta gccgtggaaa aaaagtgttg
     8581 gaatagtggt tttttctatc actttactgg attctgccgt tttttcttta tcagaaactt
     8641 cagtagtatt tatcttcgtc gtctggcaac cacttaacgc aaacaaactg ataaggacta
     8701 aagctgctgc agttgcacgt ttgcataaat ttttgattgg ttctgtcata cacctcttcc
     8761 tttccatata gaaaaaagaa agacttgaaa ggccaatcat atgatcgaca tttcaagtct
     8821 ctttattaat cgattttatc tacttgatcg aaatcaagtt ctgtactggt ttcacgtcca
     8881 aacatatcga tgttgacttt caatttttct ttttctccgt caacttctgt tacttgtcct
     8941 tctaatccag caaatgcgcc ttcgatgatt ttcacgactt cgccaacttc tacttctaga
     9001 tcattttgtc gcaggctcat accgatagaa cgcaagatat gattgacttc ttctggaagt
     9061 aatggtgctg gtttgcttcc ggctccgtgt gaaccaacga atcctgtcac accaggtgtg
     9121 ttgcggacaa cataccatga agcgtctgtc atgatcattt caacaagtac gtatcctggg
     9181 aatgttttat gaacgatttc tttttctttc ccatttttga cttcagtttc tttttcttca
     9241 ggaacaacga cacggaaaat aaagtcggcc attttcatac tttgcgcacg agattcgata
     9301 tttgctttta ctttgttttc atatcctgaa tatgtatgca agacatacca gtttttttca
     9361 aaagattcca ttgaaggctc ctcatttcat tgtaaaataa aaaaacctca gtacacccga
     9421 agtttttctt tcgcttcatt atagcactaa aagtacgttg tggctagcga gttattttaa
     9481 aatccagcca aataagcttt ggatggctgt atccattacg taaaacatca atccgaaaat
     9541 aatagaagtt tcgatgacta ctaacgtatc tttacggagt tgttttctgc ttggccaagt
     9601 tacttgtttg atttcatctt ttacgctttt tagaaatttc atgagacaac ctcccttgct
     9661 actgtttatt tcgtttcttt atgtaatgta tattgattac aatatttaca aaacttattg
     9721 atttccaaac gttcgccgct ttttccttca ctgactgatt ttgtgtagtt gcgagaccca
     9781 caaacggaac aagcgagtgc tgcttttttc tttgccattt acttctgccc ccttgctgag
     9841 aatcagactt atacttcata acagtatcac gtaacttttt acttgtcaat ttgtgaaata
     9901 aactatctat tgttttggat aaaattatga tatgattaat ttgaaattaa gctaaaatga
     9961 gacaattgga ggtatagcga tgctattaaa aacacttgta tataagaaaa aagatctgac
    10021 aaccgttaca gagactagta cattagaaga agcattaaaa attttagaaa cttccggata
    10081 ccgttgtgtg ccgatcttag attcttcagg taatattttc cgtggaaaca tttacaaaat
    10141 gcatatctat cgtcataaag caaatggcgg tgatatgacg cttcctgtca ctcatctttt
    10201 gaaaaacgct acaaagttca tttacctgga tacttctttc ttcaaagtat tctttacgat
    10261 caaagagctt ccttatattg ctgtccttga cagtgaaaat catttttatg gtattttgac
    10321 acatagtacg ttgcttaata tgctctctca atcttggagc gtagaacaag gaagctatgt
    10381 actgacaatt gcttccacag gaaagcaagg agatttggct gctattgcaa aaatcatttc
    10441 aaaacacagc agcattgcca gctgtattac tcttgatatc gaaaaagacg aatacattcg
    10501 acgtacattg atcacgctac cggctaacac tgaagcgaaa gtatgtgaaa cgatcgtcaa
    10561 ccacttagag aaaaagaatt ataaagtggt agaaattgaa aatttgcaaa accaagaagc
    10621 ataatcaaaa agcgaaaaaa gaaaacagct aatctgcttt cttttttcgc tttttataat
    10681 tagacacgct tttcaaaatc aagcgcgcga tctaaataat acatcaatgg tgctgaaaca
    10741 gccatgacga taaactcact taatgcaagc gtgccatagg ttgtccaaaa tgcttggcca
    10801 cctgttggaa ctaacatata agcaatcagt cccatgctga tggtaaagaa gatcgtgttc
    10861 aatactaaac gcatctttac atttttcact ttgttttgca ataaagctgt caggcctaaa
    10921 gaaagcaatg tttggccgcc accaaaaacc acatccatga taccaaaacc aaagaaggca
    10981 ttatagacga tgaccccgcc taaaactccc caaagcaatt tacggttaaa gacaactaaa
    11041 tgattcaaac tttctgaaat acgaaactgg atcggccctg aggatacagg cgcaactaaa
    11101 atagttaacg ctagataaag agccatgatg atgccattta caacgattac ggataaccgt
    11161 tgtctgcttt tcgttttttg cattttctct cctcttttct cctagttttt ttaacgcggg
    11221 atggttgatg aaccgcggtt atgtgaaaca aacaataatt ataacagact tttcatttat
    11281 ttcaatccgt cttatctgct tttaaaaaag caaaagctta aacaatgtaa acgtttgcta
    11341 aaaatcgtgg gatatggtat aaagtaagtg taaggggtca taagaattcc cagtaaagca
    11401 gacgaagtag gatctgcaag aacagaattg ttgtgaaact taaataaata aaaaaggatg
    11461 tgggaatttg agcaacctct tactagagaa cggtctagca agcagctgaa gatctaagtg
    11521 gacatgccga accttggaac ttctccttac gtaagtaatg gatcatgcgc ttgctaggct
    11581 tttttgtgtt tttcggtctt cggacctttt tcattttcct agacttctgc tataataacc
    11641 aagaattaat tttgtaacag atttttttga aaaaacaagt ttctttcatt caaaaaatag
    11701 aaaaagcttg ctaattattg gttaaccagc aaacaggagt attaaaatat cttgttttat
    11761 tgaatagcag gaggaaatca tgaaattcca ttatcatata ttgatcaatc catcggcagg
    11821 cagcggaaat ggctataaag tagcagaaag aattttaccg gtattaaaaa ataagcatat
    11881 tgactataca cttcattata gtgaatacaa aggacacgat gctgagattg ctgaaacttt
    11941 ggcaaaagaa acattgattc cttggataga agaagaacag aaaacaatcg atactttccc
    12001 ccttttgatc atcgtaggag gtgacggcac cttacatcag gtacttgata ctttctatca
    12061 gatggaagta gaattcccag ttgcttatat tccagcaggt tctggagatg attttgctcg
    12121 aggggcaggc cttccaaaaa atcccaaaaa aggacttcag ctgattttag cagctcaatc
    12181 accagaaaaa gtccacataa tggcttatga agaaaaaata agtgaaaaaa aaggaatagc
    12241 tgttaataat ttcggtatag gattagatgc agctatcgtc catgcgacaa atcattcttc
    12301 cacaaaaaag cgattgaaca aatacaactt aggatcattt tcgtatttat tctctattct
    12361 ccgtgcttta ttcactcaaa aaggatttcc aattctggta gatgcaggag gaaaacaatt
    12421 aagtttttca aacgcatttt tatgtacagc aacgaagcat cctttttttg gcggaggaat
    12481 cgcgattgtt ccaactgctg atgcgtctaa accagtcatt gattttgtcg tggtcgaacg
    12541 aatcaatttc tttaaaatct tgtggctcat tcttttactt atacagaaaa aacaattgaa
    12601 atcgaaatat ttccatcact acacaacaaa cagattacgt atcgtttcaa caacaccgca
    12661 acatggtcaa gaagatggag aagaaatgga aaaacgacct tttgatatca caataaccac
    12721 aactaaacag ttgttttggt gttaataaaa gaggttttgt cagaagctac atctggaaca
    12781 ccgtttattc ggaaaaaagg actgtgacac gacttttgtc acagtccttt ttttactatg
    12841 cagttaattc tttgcccaat tgggttgcca tcgatagttt ctttctttac ccaaaataaa
    12901 ttggatcatt gaaaacaccg cagcaaaagc aactaagaca caaccattga accaaaaaga
    12961 ttctgcaaat gtttgattgt aactgagtgt ttcactgatc aagttattat tttgaacaaa
    13021 ccaatatgtt ggtgtgaatg ccgctatttt gctaaccatt tctggcaagt attctgctgg
    13081 tacaaacacc ccaccgataa agcaactgcc tgtgataaaa atatctttga tcccaccgct
    13141 ggtttctgag ttttttacca atgaagtgat acaagccgaa aagctaatga tcggcaataa
    13201 aaacaagaaa gtatttaaaa tatgatacca taccgttata tcccacgagg tgtggatcac
    13261 taacagaacc attagaagta agaagccact gaacaacagc agcgagtaac taatcaaact
    13321 aaaggttact ttgcgattca agtttcgttt ggtaatgggc gcactctggt ttcgtttact
    13381 gatctctttg cgattaaatg ctagattgat gacgctgaaa ccgctaaaaa tggatagaaa
    13441 caacccataa gacaagaaat taaaggttgt gcccgtcaac agctttttca tccgctgact
    13501 atatcccgca cttagctcaa cttctccagt aaccgagagt gccatttgtg tctgttccat
    13561 agctgcatcg attgttttat cttcttcttg aaacagaatc agtgtttgca aaaaggtgtt
    13621 cacttgctgt gtaacaactg ttttagaaaa ggcatctggg cgactctgta tcttgatcgt
    13681 cggcatcttt cctacttcga cttgttcggt aaagtctgca ggcagatgta agataatgtt
    13741 tacacgatta aaataaagag catcatcgat ttctttttgt gaggttccat ccaatgtaac
    13801 gactgtttcc ttgtcattca aatatttacg aaggccatcg actacaggcg actcttcttc
    13861 actaatcaag gcgattttcg catcttgcag agaggtattt tcaacatcaa tattttgcgc
    13921 aaaaaagaat gtggcaacaa cagtgataat tacacctatc agtaaattcg atttatttgc
    13981 tttaagcaat gtccaaatcg ttttataaag cgtcataacg cacccctctt tccatccaag
    14041 catttcctaa cgcaaaaagg aaaataaatc cgatcaacca tagaagattg gtataaaaac
    14101 tagccaaatc agtgtagaaa taaagtttat acaaactttc gctgatcaag ttgacgagat
    14161 ttaattgacc aacaatcgga atatgaagat caatccaata cttcacttgt tccgacccca
    14221 tcataccagc aaaaaagctc atcgcaatcg tcactgaaat caagatgcta tccttttgca
    14281 ccagattcat ttttggtatc aaattaccaa ccaatgtacc taacaaaatc gcacacaacg
    14341 accccagagc ggtaacaatc atcaaccatt gccaacgttc accaaagtct acttgataga
    14401 taaaacgaaa gacagctaac ataatataaa tctctgtcaa aacaatcgta aagcttgctg
    14461 ccaaattagc gattgaaact aacgattgct ttctaggaat caaggacaag cgaataccat
    14521 ttgacgattg atttgcttgc tgatcctgta cattgcggac cccccacatc gtcccgtaga
    14581 gaatcgccat tccaatcaga gtgaagaaat aaaaactttt gaaactcata ttacgacctg
    14641 catgaacttc ttgaaaagat acttcttgtt caaaaacagt catgatgggc gcgagatcaa
    14701 cgttctccac ttctgccact ttactcgaaa ttataccaat acgctgtaaa aattgatcca
    14761 tgacgttttt catcaacgtc tgactcattc ctgcttgacc cacatggagc gaaacagatg
    14821 aaccatcaat gagataaaaa ccagccaaat catctgcatt tagcttttca ttcgcttcta
    14881 cttccgatag ttcctgtact tcaaataatg gtttatcatt gacttcgact tgttctaaga
    14941 ctgtggtaaa attttccgaa agcgctgttt gatccgcttt gattacaatt ccagtattaa
    15001 tcgtttcgag attgccacct tgatcaatct cgccaaaggc cgccataaac atcaatccta
    15061 aaagaattgg aaaggccaat gtccaaaaca acatggattt atttcgcagc agtatttttg
    15121 tgcgatattt ataaaaatgc caaaacatta tttcccctcc tctagtctct caaatccttg
    15181 ccagtaatct ctaaaaagac atcattcaag gacgcttctt tggttgctat ttttccgtaa
    15241 ctgatctttt cttcttgtaa gattgtaaga atcggcaaca atactgtatt tagttcagta
    15301 gttgttactt ggagctgacc ttttttataa gcgattccat cagaagctat tgttcgctga
    15361 caacacgttt tctgggagta atgggatgtc gatcgtcaaa cgatccgccg tccgtaccat
    15421 atcttttaga gattctttgg ttcctttagc aatcacctgt ccttgatcca tgatcacgat
    15481 ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt gtatagacaa tggttgctcc
    15541 ttgtcgattc agctccttga tactttctaa aattttattc cgactttggg ggtctacagc
    15601 tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg gcaatcccac atgcgatgtt
    15661 caaccgacgt ttcaagccac cactaagttc tcctggacga aacgaaacat atttttctaa
    15721 tcccactaat tgaatcacct cttcaatcaa ggctttcctt ttctcttttt cggaaacata
    15781 taaaccgcaa aaataatcga tattttcttg cacacttagt tcgtcaaaga ccgcaacgtc
    15841 ttgaggaacg accccaatca ttgccttgac agcataattt tcaggagtca ttgttgtccc
    15901 gaacaattta atggttcctt tgtcataatt taacaaagaa agcatacagt tgatggcagt
    15961 cgatttccca ctgccgtttg gtcctaacag acctaagact tctccttctt gtacttgcag
    16021 atcaaaatga ttcaatgcta caaaatctcc atagcgtttg actaatttat caatttcgat
    16081 aatcatcatg aaaaactcct tccaaatttc ttgttgatct gactataata aagtgcaaga
    16141 aaagaaacta gtgacgattg gctaaaccac acatgacatt tgtcacaatg aaggagtctg
    16201 ctatgcataa atctcggcta ctcaacgaaa ctagctttct actggttatc tcattgcttt
    16261 tgctggtaag tcaaggattc gttgctatgc aagttatcta tttattgatc gccgttttat
    16321 tcactggtgt cagttattta ctgcctacaa agccaaattg gattttccaa gttattctat
    16381 ttcttttatg tgccttttta cctgatcttg tcctgtttct gccagcgacg atgcgagtga
    16441 tcgcccctga agaaagccac cttgattact tgtttttgtt aggttttttg ttgatttcga
    16501 cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt aattttgctg tttttttcct
    16561 tttacttacg aaaaaaagat caacgaacga ccgatcttga gaaaaagtac attgtctcca
    16621 aagatactca ttgggaaaaa caacaagagt tatcggaaca aaataaagca ttggccctgt
    16681 cacaagaaac acaattagcc ttgcaaattg tggaagaacg gaaccgaatt gcccgagaca
    16741 tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt acaaattggt gcgcttgaaa
    16801 tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa tttaagtgcc accgttcaca
    16861 ctgggatgga tcggattcgt caaagtgtcc atgacctcca tgagacatcg atctcttttc
    16921 aacactcctt agagtttctg atcgctgatt ttccttcttc tgttacgatt gaaggaaact
    16981 tgtttgaagg attgaatgaa acgcagcaaa actgcttctt aatggtcatc aaagaagcct
    17041 taaccaacag tatcaaacat agtcatgcca aaaccgttgt tttacatttt cgaacgttgc
    17101 cggcctttta tcgcttgcaa atcacgaatg atggcaccag cccacaaaaa aattctgctc
    17161 ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga acaaaaggga caagtccata
    17221 ttttccaaga aaaccagcaa tttcagttaa cgatcatttt accaagggag ggacaccaat
    17281 gataacagta gtcattattg agatgatcca tttgtgacga aatcgttaca aaccatttta
    17341 gaaagcacga aagagatccg tgtgttaggc attggtcatt gtgccaagga tgcgctagtc
    17401 ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca tccgcatgcc taaacaaacg
    17461 ggcatcgatg ctgccaaaga aattttggct aaatttccca aagccatcct tttgcttttg
    17521 acgacattca aagatgaaga atacatccgc gaagcatttt ccatcggtgt caaagggtat
    17581 ttaatcaaac agaacttgca agcgattatt ccttctgtca aatccgctta taatggacag
    17641 gccgtatttg gtaatgaaat catcgagact ttcactcaaa tgatgaagaa taaaccgacg
    17701 attgatagtc atgcttatgc ttcttttagt gaacgtgagc ttgcaatcat caaagaagta
    17761 gctgccggga aaaacaataa agaaattgca gatgccttgt acttgagtga cggcactgtt
    17821 cgcaattaca tcagccaatt actagaaaag ctagatttac gggatcgcac gcaattggct
    17881 atctattttt atcagcattt gcaataagtc ttatctaaca caaaaaaacc tgaatgatgc
    17941 tccttcgcat cattcaggtt tttttcacat cttctattca attgtcccgc aaaccatttc
    18001 ctcacgattg aaagaatcaa ggattcgcag ggctgccagg attcgttgga ttgttcggcg
    18061 tgttaggcgg aaaaataggc gccttgtcta acgttttttc tagttttgat tcttcacgat
    18121 cgtctcgttc tacatcagga ctttcattca atttgaattc tttttccatc tcatctaact
    18181 ttttaaagtc attgccagat tcttttgcta atttcgctaa tttatcttcg gtttctttag
    18241 ccataaagta acgcccccct tttgatttca gcttagcata tcacactgaa aaaacaaaag
    18301 aaaacgctaa ccaacgatca tttttagcta tttctcatct ttaaaatcaa gcattttttg
    18361 ccggtataaa atcgtaggga gaactgtttt ccataccaat catcggatct actaactggt
    18421 tatcgatcac ttctgctgta agaacgaaat ctttagaaac agcaggttct acctcttctt
    18481 tcttttcttc tccaacaaat gaagaaaaca gatctggttc cactgtttcc attacttggt
    18541 ttttcgtttc attcgtccca ctgtcatctt ctacaggtag cttttctttt acaacccgtg
    18601 gctcttttgt atagttccca tcttcaaatg gatcatttcc gaccaaagaa tcttcataag
    18661 ctgctagttt ggtccggaga gtgatattca ttttatcatt atccatgatc cttgatttca
    18721 acgtagtcat ccggcttgct gattcattcg ctacacaagt gacataggct tgctcttccc
    18781 ctaacaaaat caacagttcc ttactgcgag tcactgccgt gtagagcaaa ttacgctgaa
    18841 gcatccgctg atattgatgg accatgggca aaataaccat cttgaattca ctgccttgtg
    18901 atttatggat tgagcaacaa taagaaagag tgattttatt ccattcgttt cttttataag
    18961 tgacctcatt tgcatcaaac tggataacta attcatccac tttatcttca gaatcttttg
    19021 ccaagatgat acccgtgatt tctcccatat ctccattgaa tacattcaat tcgggagtat
    19081 tgactaattg gaggactttg tctcctatcc gataaacggt gtcgttccaa tgtacttcct
    19141 tttttccagg tttccctgga ttgaatatct cctgcatcat tttatttaac gcatcgatcc
    19201 ctgcaggtcc ccgatacatt ggtgctaata cttggatatc ttgtgcacgg aatcccttat
    19261 ttttcgcttt ttccacaatt ttagagatca agggttcaat ttgataagca tcacttcgga
    19321 aataggaacg atctttttga ttctttgtga aatcttttgg cagacgtcct tctttgatct
    19381 catgggcaag aggaataata ctagaaccat caccttgccg ataaatctga ttgagttcca
    19441 ttttgggaat ttgttcgatt tccaaaagat catggagtac ttgaccagga ccgacagagg
    19501 gaagctgatc tttgtctcct actaagataa cttgcatatt cgtcggaatc gctttgaata
    19561 atgtgttagc cagccatgta tcgaccatag acatctcatc aacaatcaaa agtccgcctt
    19621 ctaattcttt cgtactgact gaaggtgttt tttcccgtcc attcagcccc agaagacgat
    19681 ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg tttagctgct cggccagttg
    19741 gtgctgcaag tagaatagga aaaatttctt gggtatattt tgaaggatcc aattccagtc
    19801 cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac agtagttttc cccgtacctg
    19861 gtcctcctgt taaaataaat agaggggaac ggattgcttc agcgatagct tgttcttgcg
    19921 aaggtccata ctgtatgccg aatatttttt cgatacgccg aatattcttc tgcacttctt
    19981 tttcaggata atggatttct tttttacgac tgagaaggcg ctggatcgaa ttaccgatcc
    20041 cccattcaga aaagtacaaa ctgttttcgt aaagattagt ctcttcttgt tgtatctttc
    20101 cttcttcaac aagttggata attacagtag caacctgatc aggctggatt tcaattgggc
    20161 gacttgtttc taaaatacgg atcgtttgct gaagcaatgt ctccgccggt acgtatgtat
    20221 ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat agctgctcga attcttctcg
    20281 gtgaatctgc tgcaataccg atttgttcgg ctagattatc tgcacgtttg aacccgattc
    20341 cttctatgtc ttcgactagc tggtacggat tttcttcgat gatatccaaa gactcgttct
    20401 tataggcctg gtagatactg aaagctaact gactaccgaa cccgtaacga ttcagtccca
    20461 tgatcacttg atccatcccg tggtttagcc gaatcgtctc agcaagcatt tctctttttg
    20521 gcttcgtcaa accggagatt tgttttaata ccgaaggatc agcaatgatt ttttcgatag
    20581 catcttctcc cagcaattct acaatttttt ctgctgtttt cttcccgatt cctgggaatt
    20641 tttcgctgga aagatacgct acgatcccat ttgcagttgt tggcttttct tgctcatatc
    20701 tttcgagttt aagctgcctt ccgtatttag gatgatccac taattctcca taaaaacgat
    20761 aagtctcatt ttcttgaata tcgccgaaac ttccggtgac tacaatctct ttttcacggt
    20821 aatcagctgt ggtttcactg atgtgtacca aaagcacttt gtaaaaatta ctaggatttt
    20881 gaaaaaaaat cgcggcaact gtaccaacga aataaggcat ttcttctggc tgcatcggaa
    20941 gccgcctcct tcttctatag taaaggttct ggtgctgttt cagttcctaa aaaatctgtt
    21001 tggttccata ctttcagttc atatggcaat aatctgcaag gctcttcagt ctctaaaatc
    21061 gtcaaactac tattgaacaa tccacccatt tcacgcaact cacttagctc ttttcctgtc
    21121 atccattgaa tggctgcagt taaagaagct ccatgtccta cgaataaaag tgggccatct
    21181 ttatgttggg caaccgcatc tgctacaaca gtttcgatac gtgtgattgc cgattcaatc
    21241 gtttctccgt gaaaaggaga aggatcatac agatctaaat gattacgcaa attcggcaga
    21301 ttatccggat atttctggta catttcatcg atcgactgac cttctaaagt acccagccct
    21361 agttctctta gcttatctgt ataaaaaatc tctaccggac gagcaagctc tttgttgata
    21421 ccttctgctg tatcacgtgc tcgctttgct gtactggaat aaattgcttt aaaaggaaca
    21481 tattttacct ttttcccaaa tgctttgatt tcttcgtaac tttgtggcaa aagcggagaa
    21541 tcgccattgc tcccttgaaa acgtctttcc tgattccatt gtgtcttccc atgtcgggta
    21601 aaatatagtt ccatacgcag caaactcctg tctttctttt attttactct ataataaact
    21661 gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt ctaaattagg gaatccttgc
    21721 tggcgaagtg cttcatacac tatcattgca gctgtattcg ataaattcaa tgagcggaca
    21781 tgctcatcat tcatagggat gcgtaaacac ttttcttcgt tttcacgcat aaatgtttct
    21841 ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct cttctccatc agcaaaattt
    21901 tcgtcactat atactttatg ggcaaatttt gagatcaaat gtagtttacg attacctaaa
    21961 aatgttaaaa aagcttctaa attactgtgg taagtaatat ttacatcatt ccaataatcc
    22021 aaccctgccc gttttagatg tttgtcatca gttgaaaaac ctagtggctc gattaaatga
    22081 agcggagtat tcgtggctgc acacgtacgg gcaatatttc cagtgtttgc agggattaat
    22141 ggttcaaata aaacgatatg attcatcagg atcattcctc tcatatttat gttcgtttct
    22201 attttctgac aatttgattt ttgcataaaa aaaaacgtat caactcggtg tcagtcactg
    22261 cgagtcgtta cgttagtgaa gctcttctca ctaactttta tcagaacagg aacctgataa
    22321 aaaccaatga aaaaacaact tgttgctaat ataacaccct caactttttt ttgcaacctc
    22381 tactcattaa aaactgctag aaaaacgttt tctcttagtt ccttttaata attctatgtc
    22441 aattgtaatc ttcttcaaag gattcttata taagtctgct ttgattcgct cttctacctg
    22501 ccattctaaa ggtgacatgg ccaaaagatc tttttgattt tcctctggta tctcaaattc
    22561 ataggttaca cgctgacgtt cagttaattt cattgcttca gcaaacttgg ctactacacg
    22621 ttcattggaa taactctgtt tttcaggttg attcggataa aaagcctgac gtagttcctt
    22681 taaataattt tcttgtggca ccaccttcaa cacttctcca tcttctgaga gtacacgttg
    22741 gaattcttgg taatgagaag gcgaaaagat atttaaaacc gcgttgaatg tttgatcagc
    22801 aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct tcggcagatt gttctgtagc
    22861 tgcgtagact ccttcttttg aaatatcaaa tccaacagca ttttgaatct ttttcaattt
    22921 tgaaagttca ttcaagaagc taccttctcc gcagccaaca tctaatagcg agacttcttc
    22981 aggtaaatgc tgcgcaatag tctcaataac gggctgatac atgccagatt ggatcattct
    23041 acgccttgga acgaacattc cttgatcgta ttctgtttta accgcatggg ataaaaaata
    23101 aagtgttcct tttttggaaa ggtcaaaacg gtgattattt ttgcatatca agccattatc
    23161 agactttttt acttcttctt ggcaaaccgg acagcggaac agctgatgat tttctgccaa
    23221 aaactggcgt cctcggtcaa ttttttttaa catagttccc cctcatttct gttttatctt
    23281 accgttagat gctcactttg gcaactgcta aaaatatgat acaatgccta gaagaaagtg
    23341 aaagataaag gagaagatta gatgatcaaa gaattttgcg ctgaaaacta cacatctatt
    23401 cctctagcaa ttgctagagg agccgatcgg atcgaattgt gtgacaactt agcggtcggt
    23461 ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa gttatgctgg ggaaaaagat
    23521 atccccgtaa tgacgattat tcgtcctaga gggggcgact tcgtctataa cgatatcgaa
    23581 ttgaaaatca tgcatactga tttgattgaa gcaaagaaat tagggacgga tggcgtagta
    23641 tttggttgtc ttactccttc aggctggcta gacgaagagg ccttagaact attgatcgaa
    23701 acagcagaag ggttgcagat caccttccat atggcttttg acagtattcc tgaagatcgg
    23761 caattcgaag caatcgattg gctggtagaa catggtgttg accgtatctt gactcatggc
    23821 ggtcctgctg acaaaactat tgtagaaaat ttcccacggc ttaaagaatt gattgcctat
    23881 acagatggcc gcttaatcat tttgcctggt ggtggtgtca ctgcagagaa tgcagcacat
    23941 gtagctaaag aattgaacgt aagcgaagta cacggaacca aaatcgtgga tcttcagtta
    24001 taatcttata aacaaaaaaa catttgcaac aggcataatg cttattgcaa atgtttttgt
    24061 ttttttacat aaaatagccc ataaataaag aagcaatatt gaaataaatc agtacactcg
    24121 tcaaatcgct caaagtcgtg ataaagggac cgcttgctac tgctggatca aatcctagac
    24181 gatccattag catcgggatt aaactacctg caagattcgc cactgtgatt gcaaatagca
    24241 tagccatccc tatgacaaac cccaagataa aattatgttt ccaaaaacct acgattagaa
    24301 aaatcgtgat ccccgtaaca gctcctgtga caacgccggt caagacttca gccaaaacaa
    24361 gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag tcgacgaaca gccacagcta
    24421 gcgactgggt tccagcatta ccggcagttc ctgtaatcag cgaaatgaag actgctaaaa
    24481 tactcgcttc acttaccaag tcttcatagt gactgattaa agaagcggtc gacataccta
    24541 aaaacaacag agtgatcagc caaggcaaac gtcgagacgc tgcctttact ggattttcat
    24601 taatttcttc cacattgacc ccggcaagtc ctgaataatc gctagcagct tcatcatcaa
    24661 taacgtcgat gatatcatcg actgtgacga ttcctaacaa atgatcatca taatcggtaa
    24721 caggaagagc taagaagtca taatctctaa atgtttgtgc cacatcttct tggtcgtcgc
    24781 ctacatgaac tgaaagcact cgttcgctca tcagatcaga aatcattgcg tcgtcatcat
    24841 tcacgattag gtcgcgcaga gaaataaccc ctactagctg accttcttga ttaacgacat
    24901 agatgtaata gattgtttca gctacatctg cttcgttctt cagcacgtac atcgcagagc
    24961 gaacagtttg attggctacg atcgaaacaa attcagttgt catgatcgcg ccagccgttt
    25021 catcctcgta atggagaagt tccttgattt cactggcatc gtctgccgac atcaaactta
    25081 aatatttagc aatttgcttt ttatccagcg tattaagcaa gtcaacagca ttatctgtat
    25141 acatctcaga aagcatatca gctgcatagc taggacgcat ttcatctagg tattctttca
    25201 tatgttcgtt gtcttcttca atcacatcga acatatccgc taattcttta ggcgacagat
    25261 aggaatagat cagctgcctg ttctcttctg taagagattg gtagaattgc ccctgctcat
    25321 agatatgcat ctctaaaaaa ctgtttctga atgcctgaat attattttcc cttaaatctt
    25381 gagtcaactg ggcaaatctt tcctctaatt cttgattttc atccaaacca atctcacctc
    25441 gccttattct tcaaaccgtc tcacaactgc ctccatgtct gcggctaaag gctgcttgaa
    25501 ctccagcaac tcttctgtaa atggatggta aaaacgcaga tcataacaat gaagggcttg
    25561 tctttttatt cccagatcca ttcgcccgcc atacatatca tcaccgagca gggcacagcc
    25621 aattgcggaa aaatgaactc ttatttgatg tgtccttcct gtatgcagct ggatatcaac
    25681 taacgcgatt tctgttagtc gcttctctat ccagtactca gtctgtgccg aacgtccttc
    25741 atcagagacc attcgcttca atagcgaaga ataatctcgg gcgatcggca aatcgatatg
    25801 tccatgtgcc gataaatctg aaagccttcc actgactaag gcctgatatt ttttcacaaa
    25861 cttcttttca cgaagttgca tatctaattt tgcgtgcgca aatccatgct ttgcaaaaag
    25921 catcaaacca gatgtatccc gatctagtcg cgtcaccaca tggattactt ggttttcgta
    25981 accttgtttt ttataataag cctttacacg attagccatt gtcccatttg gatggtattg
    26041 ggcaggaata gacgaaacgc ctgctggttt atttactacc agaacgtgtt catcttcata
    26101 cacgatatcg atcggtgttt catccaaaag tacggtttca tgttctcctt cagcaggaat
    26161 cacaatggtt accttatcat ccttagctag agaaaacaag acattttcta cttgatcatt
    26221 aactttgatt tgaccgcctt gaaattttat tttcgctaac agaccctttg agatcccttt
    26281 ttcttttaaa aaatatttta cttgctgaga atgatctttc tcataatccc atgtaaactg
    26341 catatcaatc ctcactaata aaggcatctt tcacacgatg ccaaaaatgc atatgtcgat
    26401 aagaagcaaa atggatccgt tcttctgcta ttcgataata aattgagcgg atatttgctt
    26461 ttgcgacatt caattgatca accgttacaa gataatcttc tgtattttcc aattttattt
    26521 ctagccattc atcatgggca atcacgatcg gagaacccaa agtacgaaac actcgattat
    26581 tcagtgaagc aatctctgcc aactgaaagg cattgatact aggatggatc acagcaccgc
    26641 cgacactttt attatatgct gtcgaaccag taggcgtaga aatcgtcaat ccatctcctc
    26701 taaaactttc aaacagctcg tttttgatgt atacatcggc taccatcgta cgattggccc
    26761 gtttaatcgt tgattcattc agtgctaaaa aatgctggtc cggtgtttca tccaaataac
    26821 tgatccgaac atccagcaag ggataactaa cactttgttc ccggttcgtt cgtaagctgt
    26881 ctactagttc ttccagctcg taatcccgcc aatccgtgta aaaccctaaa tgacctgtat
    26941 gcacgcccaa gaaacgcaca tcattcagac aatgactgta gtgatggaac gccgacaata
    27001 atgtgccgtc tccgccgact gagatgacca attctggctc atgatcatct cgttgaatcc
    27061 ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc tatagtcttt gcttctttat
    27121 tatgaacgat cgctactttc atcccctgcc tcctctcttt cgctttcttt cttcaaaagc
    27181 gttcgatagt agacatcatc tgattttcct cttccatggg agaataaccg ctgtgcttct
    27241 tggatttctt cacggatact ggacatctct tcatccaact gataggcagc ttctgccgca
    27301 cgctgaagtc gttcactcat ttcttctgga aaaacgcctt gatatttata attcagcgaa
    27361 tgctcaatcg ttgcccagaa attcatcgca agagtccgaa tctggatttc cgccaaaatt
    27421 ttcttctcac cgctgatcag ctgtacagga tactcgatca ccagatgata agaacggtag
    27481 ccacttgctt ttttatttgt gatgtaatct cgttcttgga gaactttcat gtcttttctt
    27541 ttacggatga tctcaacaac ttgatggata tcttctacaa actgacacat gatccttaac
    27601 ccggcaatat cagacatttc ttcttctaaa cgtcccattg gaatatgccg taattgtgat
    27661 tttgttacaa tactatcgac tggcttgact ctcccagtga caaattcaat cgggacatgt
    27721 cgattttgtt cacggaactg tttgcggatc ccccgcagtt tcactttcaa ttctgataca
    27781 gtttgttcat atggtgctaa aaatgcttcc caatctcgtt ccatagctga tacctctact
    27841 tcattaacat tcattgttta ttctaccata tatgcaaagt tttttcgtac tcttgattta
    27901 tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa aaggagtcga tcaaatgaca
    27961 caaatgcttg aaatcgaata taaatctatg ctgacaaagc aagagtacga gatattcatc
    28021 gctcactatc aattaacaga taaagatttc cgtgtacaga ctaatattta tttcgatacc
    28081 gttgatgaac agctgaagaa acaaaattgt ggcctacgga tccgtttcgt tggtcatcaa
    28141 gctgaatata cattaaaaac acctgctgaa aaagggcgtc tcgaaacaac ggataccttt
    28201 ataacagaag aagcagaagc atttatcttc gaaaaacgtc tgcctcgttc aggcgcagtt
    28261 tttcaaaaac tttctgattt gaatatcgat cctgcttcat taatccaaac cggaaagctt
    28321 acaacaaaac gggctgaatt ccctattgaa gaaggacttt tagctattga tgaaagctgg
    28381 ggagacaatc tacacgattt tgaactggaa ctagaggtag gggatgcatc tgtgggaaaa
    28441 attgctttta taaatttttt aaaacgtttt tctttacctt atcgtcctgc taagaacaag
    28501 atccaacgta tgcttgaagc taaaaattag aaacctttca ttttcacaag gcacttgagt
    28561 tcattttttt acttttttct gttaagttat aggatgtgaa agaatctagt cactagttgt
    28621 atagaatcta ttttggggga gattcatatg atcgaaatct atttatttgt taatccttta
    28681 ggggaatact gtttcgaaat ggaacaacag ttattacaat ttatcgaaga agagtacggg
    28741 acaccatcaa aagaaaagat gcaatttcgc tttcttcctt tggtcaattt gcagacaatt
    28801 ggggatgtca tgcaacaaaa aggaatttct cagaacaatc ttgaggaacg taatcactta
    28861 ttctcaatga cttattctgc agcattggac tgcaaagcgg cgcagtttca aggaaagaaa
    28921 aaaggccgcc gttttttgat caaattgcaa gaagcagtcg gctgtaatga cataccatat
    28981 tctcaagaac ttgctgaatc catatttgaa gaaatcggcg gcgatttgaa tatgtttaaa
    29041 gaagatcgtc agtctgactt cgttaaagaa gtattctttt ctgatcaaaa aattgctcgg
    29101 gaaatgggca tcacaaaaca cccttctgca gtcgtttaca attatgcctg cgaccgtgat
    29161 tttggtgttt tagtagaagt aaaaggtaca agtagccttt ggagaatcac agagctgtgt
    29221 aaaacaacag ataactcata tcaagttttt aatggtgaac atttacatac aaatgattct
    29281 cagttccatt caagaactag ccatttacac ttgctttcta attaaaaaaa tacgtcgact
    29341 cccttacaag agagccgacg tatttttttc gtttctattc gagaaaatca aaaatagaaa
    29401 gccttataaa cgatcgatca attcttctaa ttcatttaga cgttgttcaa acatcgacat
    29461 tgcatcttca atatatgcag cctgtgtcat atcaactcct gcttttttca ttacttccac
    29521 aggataatcg ctgttacctg cttttaggta agccaagtag ttctctagtg cctctggttc
    29581 ctggtttagt attttcttcg ccagtgctga agcagcagaa aagccagtcg agtattggaa
    29641 aacatagtaa ttataataaa aatgcggtat ccgtgaccat tcgaatttaa tttctgggtc
    29701 ttcttcgact gctggaccat aatattttgc attcaattta ccataactat cacttaggta
    29761 ttcactagtc aatggcacac ctttttcatc ttccgtatgc atgaaatgct cgaattcagc
    29821 gaattgtgtc tggcggaaaa ctgttccttt aaacccatcc aagtaatggt tgagtacata
    29881 agcccgtaca cgagggtctt tttctgtttc caataaatac tccgttagga tattttcatt
    29941 cgttgtcgaa gcaatttctg ctaaaaagat ggagtagtcg ccgtacacat aaggctggtt
    30001 cgaacgagtg aaataactat gaacactatg tcccatttcg tggacaagcg taaatagctg
    30061 atccagcgta tcatgccaat tcaataaaat atatggattt gtgtcatagc ttcccgaaga
    30121 ataagcaccg cttcgtttcc ctttattttc gacaacatcg atccaacgtt cagagaatgc
    30181 tttttctacg atggccatgt attcttcacc cattggtttc agtgcttcta aagctttttc
    30241 ttttgcttct tcgtacgtaa aggtaattgg agcttcccct aagaccggtg tataaagatc
    30301 atacatgtgc agtttttcca cttctaataa acgtttccgt aattccatgt atcgatgtaa
    30361 caaaggcaaa tgcttgttta ccacgtctac caaagtatcg tatacacttt caggaatatg
    30421 attattgctc aaagacgctt ctctggcaga gctgtaatta cggactttcg ctttaaaatt
    30481 atgtcctttt atatgtgtgc ctaaagtaga agcaaatgta tttctaaatt gttcgtaaac
    30541 actgtacaat cccttaaatg ctgcttcgcg cacccttcga tccgtgcttt ctagcaactg
    30601 accatacacg ccatgagata attggactat ttcaccattt tctccttcaa tcgttggaaa
    30661 aactagatct gcattattca aaacagcaaa tgtatcactt gaagcatcaa agatttcacc
    30721 tgctccagca agaagagatt cctgctcagc agataagaca tgggctcgat tatctactat
    30781 ttgctggata taatggcgat agacttccaa ttttggttct tctttgaaat attgccaaat
    30841 ctggtcatct gacaattgca atatttctgg ttcaaaccag gaaacggctt cactaacttt
    30901 cgaaaacaga ctgcttgctc ttgcataaag cgcttggtaa tctgtatttc cagtgtcttg
    30961 atcgttttta aggtgcgcat atacataaat gacttcagtt tggcgataaa ccctcaatac
    31021 gaattcaatc gcatttaaaa attgagaagc gccttgatca agtgttcctt tgtgtttttc
    31081 agattgtttt aactcttctg ataattccaa atatttctca tcaaactctt ggtcgcttga
    31141 aaagatcttg gtcaagtccc aagttaaatt ttcaggcaat tcttctcgtt ttggcaactg
    31201 ctttacttcc atgaaatcca ctccttttct tcccttagct ctatctatca aaatagatca
    31261 tagtctaaca tccacatgtc acagcatcta acttttctac tcattcatgt gtctgcttcc
    31321 atcaatttta gcataactaa atcttgtttt acatagaaaa ccgattccta gcctattttt
    31381 atacttacta ttttttcaaa gaagacaagt gtatgcattc gttaaaaaac tgctgataga
    31441 ccatctcttc atcaatcata ggaaaaagcc attccctttt atgttctttt atttggcaaa
    31501 gccatgagtt gaaatcagga actttctggt tttccaaggc ttcttcgtac aacattcgat
    31561 aaagaaaaac ctgttcttgt aaataaaaga aaaaccgact tttttgatag atccacgatg
    31621 atagatgcaa aagatgttt
//
LOCUS       DACEPW010000011        31337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEPW010000011 DACEPW010000000
VERSION     DACEPW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.011"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..490)
                     /locus_tag="IW819_000409"
     CDS             complement(23..490)
                     /locus_tag="IW819_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1243541.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(610..2148)
                     /locus_tag="IW819_000410"
     CDS             complement(610..2148)
                     /locus_tag="IW819_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1243542.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2228..2914
                     /locus_tag="IW819_000411"
     CDS             2228..2914
                     /locus_tag="IW819_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1243543.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3115..3642)
                     /gene="msrA"
                     /locus_tag="IW819_000412"
     CDS             complement(3115..3642)
                     /gene="msrA"
                     /locus_tag="IW819_000412"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1243544.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3726..4403)
                     /gene="pgmB"
                     /locus_tag="IW819_000413"
     CDS             complement(3726..4403)
                     /gene="pgmB"
                     /locus_tag="IW819_000413"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1243545.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4400..5530)
                     /locus_tag="IW819_000414"
     CDS             complement(4400..5530)
                     /locus_tag="IW819_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1243546.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQR
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5530..7713)
                     /locus_tag="IW819_000415"
     CDS             complement(5530..7713)
                     /locus_tag="IW819_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1243547.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVMNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7710..8735)
                     /locus_tag="IW819_000416"
     CDS             complement(7710..8735)
                     /locus_tag="IW819_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1243548.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8780..9607)
                     /locus_tag="IW819_000417"
     CDS             complement(8780..9607)
                     /locus_tag="IW819_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1243549.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLGDNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9604..10671)
                     /locus_tag="IW819_000418"
     CDS             complement(9604..10671)
                     /locus_tag="IW819_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1243550.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10697..11533)
                     /locus_tag="IW819_000419"
     CDS             complement(10697..11533)
                     /locus_tag="IW819_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1243551.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11530..12399)
                     /locus_tag="IW819_000420"
     CDS             complement(11530..12399)
                     /locus_tag="IW819_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1243552.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQTVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12469..13752)
                     /locus_tag="IW819_000421"
     CDS             complement(12469..13752)
                     /locus_tag="IW819_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1243553.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDISKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13766..15451)
                     /locus_tag="IW819_000422"
     CDS             complement(13766..15451)
                     /locus_tag="IW819_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1243554.1"
                     /translation="MYTKLLQAIYRDSDLAEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVKYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15726..16751
                     /locus_tag="IW819_000423"
     CDS             15726..16751
                     /locus_tag="IW819_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1243555.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17346..20999)
                     /gene="rpoC"
                     /locus_tag="IW819_000424"
     CDS             complement(17346..20999)
                     /gene="rpoC"
                     /locus_tag="IW819_000424"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1243556.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21078..24695)
                     /gene="rpoB"
                     /locus_tag="IW819_000425"
     CDS             complement(21078..24695)
                     /gene="rpoB"
                     /locus_tag="IW819_000425"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1243557.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25423..26391
                     /locus_tag="IW819_000426"
     CDS             25423..26391
                     /locus_tag="IW819_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1243558.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKSPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26409..27314
                     /locus_tag="IW819_000427"
     CDS             26409..27314
                     /locus_tag="IW819_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1243559.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27311..28117
                     /locus_tag="IW819_000428"
     CDS             27311..28117
                     /locus_tag="IW819_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1243560.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28274..29242
                     /locus_tag="IW819_000429"
     CDS             28274..29242
                     /locus_tag="IW819_000429"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1243561.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29559..30083)
                     /locus_tag="IW819_000430"
     CDS             complement(29559..30083)
                     /locus_tag="IW819_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1243562.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30370..31337
                     /gene="scm"
                     /locus_tag="IW819_000431"
                     /pseudo
     CDS             30370..31337
                     /gene="scm"
                     /locus_tag="IW819_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
BASE COUNT         8783 a         6773 c         5909 g         9872 t
ORIGIN      
        1 cggtcaagtt caacaacagt ttttacgcgt cgatctctgg cgctttgccc aaatgagctt
       61 ggatcatcca gatacgtttt tctgtggctg ttttgaattc gataaagata tcttgtgttg
      121 gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg gtaaagatct gctagataac
      181 gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt acgatcccag cggccaattt
      241 catcagggat ttttgtgtta tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta
      301 atgtgatcaa gcgttcggat acttcatcta actgagcgtt caaatcatcc atgaactcat
      361 ccatcattgg gtgcaatgta aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa
      421 caacaacaga catttggctt agatctgcaa ctaattgatt taatacttct ttggtttgtt
      481 ggaatttcat ctttcttgcc tcctaacatt ttcttacact taaaataata gtagactttc
      541 aatgaaaagt acaacaaaat catttacctg agaacgattt tcagttcgct tttttatccg
      601 taaagccaat catttttcag aatcagctaa ttcttctcgc agttgagcaa attgctgata
      661 agtctgtgct aaatgctttt gtgtcgaaac aaataatgga tagattttct gatatttttt
      721 atgatcagtt tcttgtactc caacttgctc tttcaattct atcaattcct gtgcttcact
      781 gatactttgg atctttccga tgcttctcca gcctaaaatc acagctccta atgcggatgc
      841 ttctgtgaca gtagggaaaa caagcgggta acccagtaca tcagccatca tctgacgaaa
      901 aatcggagat tgagcaaacc cgcctgtagc acggatctct gtgagctgat tttcctggca
      961 aactcctttt aaaatccgat aaaggttgag acagatccct tctagaatcg ccttcatcat
     1021 ttctttttct gtatgttgcc gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca
     1081 aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc agaccattgg cacccgctgg
     1141 cacaaaagcg atagccgaaa gcagtgcatc atatggattt tctccttttt ctttagcaga
     1201 ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca aagatgccag caccattgct
     1261 agtcgctcct cccactaccc aatgtttctg atccagaaca taacaaaacg tctccatttc
     1321 tggatggaac tgaggctgag tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa
     1381 agcggcgatc gtatcaccaa aagcacctaa ccctagatta gaaagcggcc cgtctgcccc
     1441 tcccaaaaca aatgatatat catttggcaa acctagttct gctgctgttt ttgatggtag
     1501 acctgttact tgataggtgg aaggaaagac ttgcggcaat tgctctattc gaatgttcaa
     1561 gtagtcaagc acttcttctg cccattggaa ttcatggata tcaaaatatc cagttcctga
     1621 agctgttccg tagtcgctga tcagttcgcc aaacaatcta taaaagatat agtctttgat
     1681 accgacaaaa taagcagttt ttttgaataa ttcaggttgg ctgttttgca gccacttgat
     1741 tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc tgccgataaa attctttgcc
     1801 aagcacggta tatttcagtt tttctgcttc gtccgctgct cggttatctg cccaagtgat
     1861 cattcgagat aacggtgttt tctcttcatc caacaatatc aggccatgca tcgcagagga
     1921 gaaggagaca aattgaatga ttgtgtctga aggcaactgt ttaattactt cttttataac
     1981 ggtgcagaca ccttgaaaga tcaattcctg atcttgttct gccatccctt gtgtttcttg
     2041 gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac
     2101 tgctttggtt tgtgtggttc cgatatctaa tccgattgaa acgttcattt tttcacctca
     2161 aattttatgt atgtacaaaa atttagttta agcttgcgta tcttggtttt gaaaaggggg
     2221 agatggcgtg ttactttatt ttatcattgg ttgctgtatc ggatcatttt tatgtttaac
     2281 cgcccagcgg attccgttag gtcactctat tatttatcct agatctcatt gtgtcaaatg
     2341 ctgtcgttcg ctttcttggt atgaattgat tccgattctc tcgatcatcg tccaacgatt
     2401 tcgctgccgc tattgcaggt gtcggttgcc cttctactat ttgttggcag aagccttatg
     2461 tggtggtctt tttgcctggt tctttacttt ttcttccgca agaaattttt cgacgttcat
     2521 atggatgctt tcggctttgc tattttcgtt gatggattta ttttacttca tggtccattc
     2581 tcacacgcta ttctgttctt gggcaatttt atggattttt tggttgcagg ccggcgtctt
     2641 ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt gtgtgccttc gctatggtca
     2701 atcctatctc ggagctggcg atactctgtt gatgctttct tggagtggtg gcttgtctct
     2761 agaagagttg cttcagatct tgtttcttgc tagtctgttt ggaatatccg ttttttctgt
     2821 ttactttatc tgtcataaaa aaaagttgga aaagctgcct ttcattcctt ttttgagcag
     2881 tgcgttgctg attgtccttc atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc
     2941 acaacctctg atatatagtg ttctcttacc aaatccttgt gacttggagc taggcgacag
     3001 gttcacaacc tctccactaa atggtgttca atcagctgac cctcagtatt agctcaaaat
     3061 gctggtttta caacctctga tatacggtgt tccataagta gctttttcgg cttcttattt
     3121 cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc gataaagatt
     3181 gtaatggagc ttgtttttct tgtaataatc ctgatggtaa tcttctgcag gataaaattc
     3241 gcttcttggt tcgatttttg tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc
     3301 ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca ttatagtaga tcactggacg
     3361 ataagaatca ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca
     3421 atagatttcc accaactgat cataagaaat aacttctgga tcatattcga tctctactgc
     3481 ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa gttggatttg gtacatgccc
     3541 gccagtataa ccggatgtca cagaaatgat ccctggctgc tcttcaaatg gtttgaccat
     3601 acaccaaaag catccgcctg cgaaaactgc tttttttgtc atataaaatc cccctttttt
     3661 ctcatcatac cttgatttcc aaaaggagga taacgttttg cttgtgacag aaaaaacgga
     3721 aactattata tacgtgatac ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa
     3781 acggttaaat caggaatcac atattcagca gaaggaagat ttatgttttc acctattccc
     3841 aatacatgca tgttaccagc caaagctgct tgacaaccag cttctgaatc ttcaatgacc
     3901 aagcatgcat ttggcggtac attcaattgc tgtgctcctt tgagaaaaac ttctggatca
     3961 ggttttgctt ttgatacttg cgtgccgtca ataaggacat caaaataact tgtcaaattc
     4021 gttttttcta aaatgaggcg tgcatttttg cttgctgatc ccaatccgat cttgatgttt
     4081 gtttttttca agtactcaag aacagctatt gcaccaggca aaacggatgt ttcatccatt
     4141 ttttgaatcg cttgaagata tagattgttt ttttgctcag caaaggctga tttctctttt
     4201 tctgagaatt tctgttccat ttttccaaat gacagcaatc gctctagtga ctcggttctg
     4261 gatattcctt tcagttgctc gttttctttc tcagtgaatg gaatggacag ctggttagct
     4321 aaaaccagcc acgcttcgta atgataacga gctgtgtcta caagaacgcc atctaaatcg
     4381 aatagtgctg ctttgatttt catcgattac ctccttgaaa atgaacgact tgaagtaaag
     4441 atgcgatgtt tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta
     4501 aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat gccaacatct gctgcttttt
     4561 cgcttccttt accaacactt ccgcaaatag ctatcacagg aacgccctct ggtactcttc
     4621 tagcaatccc aactggcgct tttcctttta gactttgtcc atctagacgt ccttcgccta
     4681 cgattacgag gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt
     4741 cgatgccaga gactattttc cctgaagcaa atgctaacag tcctgcaccc attccaccac
     4801 cagcactact gcccgatgcc tcgattactt gaggggcaaa tctcatataa aaatcatgca
     4861 tggcttggtc agccactgct aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa
     4921 aagaagcgcc ttcttttccg cataaaagat tagtaacatc agtaatgaca gtgattttgg
     4981 ctttaatcag ctcaataggc acgttcgttt tatcaactgt agcaatttta cctagatttt
     5041 ctcccagtgc ttctactttt tgattatctt gatcgtaaaa agcataacct agaccttctg
     5101 ccatgccgat gccgccatcg ttggtactac ttccgccaac gccgatgatg atgtgtcgaa
     5161 caccttgttg caacaattgt cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg
     5221 gctgtcgttg gtttagtggg acatctttca atccgacgat ttctgccatt tcaaaaatcg
     5281 cagtttgacc gtctgaagcg tactggattt ctttgttttt gccaaatgca ttagtaacga
     5341 ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc
     5401 catcgcctat aggtagcaga tcatattcag cctgaacaaa aacttcttga aaacctgatt
     5461 gaatggcttg tgctgcctct aaggctgtca gactttcttt gaaagaatct ggcgcaatca
     5521 gaatacgcat tattttcccc tccgttccaa gatcagttct ttttccagct ggtattggtc
     5581 tccattgaca aaaataggta atgtttcttt cgtctctttt tttattacta tttgtttttt
     5641 tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca
     5701 tgctgaagga agtcttggag aaatagaaag agttccattc attactgtaa tacctgaaaa
     5761 accaaatata actgctagcc atagtgctcc taatgaagca gcatgaattc cgtcatctga
     5821 tgaatgcgga tctggtccta aatcaatcag gcacgcttct tggaataaat gatacgccca
     5881 ttccatttct tggatacggc aagcctctat ggcgtgaatc gctttactca acgatgagtc
     5941 gtggattgtt ttgttttcat agtaatgcaa attcttagaa acgattggtt gagaaaaata
     6001 ctcaggtaat aagtataaca gcatgacaag atctgcttgc ttgctgactt ggagttcatt
     6061 tacttcgcta cgggcataat caagtaaaat agactggctg ccaggacgtt ctttgtaagt
     6121 ggtcaaatca ataactggct tactgagata cgtgtcatct tgcggaatca gctgttcttt
     6181 gttaggcact ggcaaatata acttatctaa aaaattttga cattctttca aaaaaagcgg
     6241 atcgccagct tgatatttaa cagctaaggc cacattataa tgcgccagat agttcgtata
     6301 tgcattattg tcgatatatt ctgtgtattc atctggacca atgactctgt gtatctctaa
     6361 atgatcatcg acttttacag ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt
     6421 ttttcccttt gtttctaaaa acgattgatc ctctgttgct tggacatact cttgaacggc
     6481 ataagcaagg tctgcactga tatgatgttc tgcggcagct gaagcaactt cttgtcttgt
     6541 tccggttttg atattcaatg cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct
     6601 ttcccatggg aaaagtgcac cttgatagtg ataatctctt gctttttgcc gtgcgccttc
     6661 taatcgatca taacggtaca gcagcaaatt ctttgctact tcaggaaact gatataaaaa
     6721 gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc cctttataac cttcgccagt
     6781 tagtcccttc gcccctacac taacacttgg atcattgcgg ggagtcataa tctgtaaatg
     6841 gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa tcgccttgga tcgttatccg
     6901 atgctgatgc caaaaatctg tccaataaga catcgactgc tctaaaattt ctgtataact
     6961 aacgcttttc tgggcttggg caatactttt cttaaacggg tctgtttgct gaagagaggt
     7021 agagagcacg atgatcttct ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa
     7081 ggaagcggtg atccggcgat tttttgcttg gaatacacct gctttattga atttagctgc
     7141 aatcccaata tcaattttac tttctgttgt ctggtaggat gcactgatca aatcgtcacc
     7201 aaatactcgc agttcttttt caaccaactg ctgtgtacca aaattggttt gctgtccatc
     7261 gatacccgtt ttcacatcta cttggcagtc ttcggtcaac ggcttgatga ccactttcag
     7321 ggctgcctga tggagattat tttgatcagc aacacgacaa gtaatgactt ccactttttt
     7381 tccttgagca gtttcaatga gcgcatagcg tttaagctct cccatttcta aatctagtgc
     7441 ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat
     7501 ttcccatctg atgacatctg ggagattcac taactcggct gattctgctc ctaaaggtcg
     7561 gttatataca ccagctgcaa acattcccct tacttgattc cgatagtctt cctcctggct
     7621 tcctctgata ccaaaaaatc catttccaat ggtcatctga ctcgcatact tattgatata
     7681 ctctaaatca aactgttttt cttctacttt cataaggtaa taacttctcc tgtttctgcc
     7741 gaacgatata aattttccac cagattctgg atgacatatc cttgccagcc atctgcaatc
     7801 atctgagtct ggtcaccgag cgcccgattc acaaaatgag ccatacttag ttcatgtggg
     7861 tcattaactg gcgtggtctt ttgtaaggaa accagctttc catctacatc ttcgaatagt
     7921 tcgcctggat acagattagc acctgcctga tcgccacaaa aagccacatt cagtttgtct
     7981 tctctgatat tcaaagcaaa cgaagtattc agttggatga tcttgccgtt ttctaattcc
     8041 agtgtgccaa aaattgcatc ttctactgtg aatttgcttg gatcccattt cccgaatttc
     8101 cctgaatttt ttttcgtacc aattttttgg aagctgtacg cccgaacgct ttttacttta
     8161 ggaaatccag taatatacat cgctgtatca agcatgtgaa tgccataatc gatcaaaggc
     8221 ccgcctcctt gaagttcttt gtcgataaac gttccccagc ctggaacacc agaccgacga
     8281 agcgcattcg cttctacgta ataaatatcc ccgagaacat caagatgatt cttgatcatt
     8341 gttgcttcgt cagaaaaacg atgctggaat ccataagcca aaatctgccc gtttttatcg
     8401 gctgtctctg ccatctttct tgcttgatca gctgtcatag ctggtggttt ctcgcacatc
     8461 actgaagcat tagcttcgag ggcagccaat acatgttcga agtgaaagcg attgggtgta
     8521 cagacactca cgatatcggg agaaatctca ttcagcattt gtgtaactga tgtatacgca
     8581 tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg atcaaccaca
     8641 gcggccaatt ctaagacatc tgatcttttt tgataataag cagcatgtac tttttctgct
     8701 acttgtccag cgcctatgat tgctactttc ttcatttatt tctccctcct ttgcttattt
     8761 acttattgtt attctttctc tattctttct ctaaatcatt taaatgacgc gctgtttcca
     8821 gacagttttt caagtattgc aatgattcct gataaatttt cacttcattt tctcctcgca
     8881 aacgacattc atataccata taaccttcat actcattttg aagtagcgtt tgaaaatggc
     8941 gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg attatctcct aaatggatat
     9001 gacccacata ctctttttgc gtagtcaatg cttttgaaat atcatcttct tcaatattca
     9061 tatgataaaa atcagcgatg atctgcacat gtttcaattg attagagtca atgtaatgac
     9121 gcgcatccgc taacaaattg atcatatggt cttggtatcg gttcaaaggc tccaaataaa
     9181 taaccgtacc tgtttcttcg gctactttat ctaaataacg aagtgattcg ctgactgctt
     9241 tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg taatcgataa gtgaacattc
     9301 cccaagctgc tgggataacg attcctttgc ctccaacctc gcttagtgca cgcaaaatct
     9361 cagcaatttg acgtacacca ttaagacgcc gttcttcgat aaagtcaccg atccacccat
     9421 cgtagccgtt acaagctgtc actactggga tccctgtttc tttgattgcc tgtttgactg
     9481 tgtcgatatt ttctattaac aacttaccgt cgatttcgta ttcttcaaat ccacagtctt
     9541 tgataaatct aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga
     9601 atttcattgt tctcctcctc ggacttgaac gcccattttg atactttctt ctgggtgctg
     9661 gtctacataa cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg gatcaatgat
     9721 ttcttcacaa tccaagtaac cgttcatcaa taattcccaa catgtgtcct cgattcgttt
     9781 acggttccat cttgggtaat ctgggtttgg ttcactagat gctcgagcaa agacgatttt
     9841 tgcattattg aaatgtgctt cgcgaccaag attaaaccca gctggaaaag gtttagcaaa
     9901 ggcaacataa gaaatgatcc cgccatagcc gatccctctt aaagaagctt gtaatgcggc
     9961 agcactgcca ctcgtttcaa taactgaatc tactccacgt ttatctgtca attcctttag
    10021 acgaaaaccg acatctgctt tcataggatc tagcgtgtaa tcggcaccgt atttttctgc
    10081 taattctcta cgctgcgcta tgggatcgat cccaacgatg gtagtagcac ctgctttttt
    10141 agctaattga atcgcaattt gcccgatagc gcctaaaccg aagattgcta cagcatcacc
    10201 tgctcgaaca tgtgcttccc ggataccacc taaagcgaat tgagcaggat cgtaacatac
    10261 tgcatttttc cattgatcca accgatcaag tttacgtagt ttataattat caacaccgtt
    10321 tgtaatcagc gtttcacgaa tgggtccata agtgcagact aactctcctt tttgatactc
    10381 tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc atatttccta attgaaattc
    10441 gccaaatacc acaccgcgtt ggcttccttc ttctcttggc aagaataggc gccattcttc
    10501 tgaaaattct tcatcgataa atggacttaa tcctctaaag tcgacaattt ctgttcctgg
    10561 ttttggagta gcgaaaatta ctcggatacg tacttcgttc ttttcgatct cacggtcttc
    10621 gtaatctttg aatccggcta ttcttggttc taatgcaact aattttttca tcttttcttt
    10681 cctctttctt ttctttttat ccttttacgc cgccttcgac taaatcacct ttaataaatc
    10741 gttcagataa cgcatacata atgatgactg gtaaagcggt caccaatgaa gcggccatca
    10801 ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa
    10861 atttttcgga ggtcgacagg aaaatcgaag caaataaata atcattccac gaaaccataa
    10921 agcaataaac gaagacagaa atgattccag aaatagccaa tggtataatg atccgaataa
    10981 tgatctggat ccggctaaga ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg
    11041 tgtcaaaata actttttagc atgaatatcg cagtaggaag tgtttgaacg accatcgtga
    11101 taatcagtgc tgtattggta tcatacaaac ctagaccgga aatgatttta aataatggaa
    11161 caacgatcaa aataccggaa aacatataaa cggtgtaaaa gctagcattg attttcgagc
    11221 gagctttgaa gcgtagtttt gacaaagcgt atgccccgct gatcccaatc actactgcag
    11281 ttacggaggc tatcaaggag atcactaaac tatttttgaa atacatgatg aatggaaaaa
    11341 tctcagcatt gaaaatatcc tgataatgtt caaaagtcca ttctttcgga aaaatagttt
    11401 gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg
    11461 gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct
    11521 ttttattttt catttcaagt ccaccttctt tcgtgagagc aagacgaccg ctgcaataat
    11581 gatgaacaat agaatcgata tagctgctgc ttttccaaga tcattgaacg caaaggctgt
    11641 ctgatacaaa taaacgccta agatattgac tttattcgtc agtagataga catctgtaaa
    11701 catatagaac atccagatag atcgtaaaac aatcacagtt gctagtactg gtttgattgc
    11761 cggcagtgtc acgatcttga acttctgcca agcatttgca ccgtccattt cagctgcttc
    11821 ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg catacggata
    11881 gtatctccag atagcaaaca aaacgaccat aaagaagcta gcaactgtct ggtcgaacca
    11941 cagcggtgct tcgttgaaaa gctgcaacct atctactaac agatcattga cgattccata
    12001 actattattg aacatatatt tccaagcaaa aatcaaagaa atagatggcg taacataact
    12061 taataaaatc agtgaacgca ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc
    12121 tacggctaat cctaaagctg tactgcctgc tacaactaag aatgtgtaag caactgttaa
    12181 acctaaagaa cgataaaatt cactatcact taaaatttca agataattgc ttaatccgat
    12241 aaaaacggcg tctgagttag gattcagcgg catatcgaaa aaactgatct gaatattgga
    12301 aagcattgga atcagcacta aaacagctaa taaaagcatg cttggtgcta aaagtcccat
    12361 ggctaatttt aagtcagaat gtttccttgg acgattcatt gaaacctccc cctgataatc
    12421 tgccaatagc tatctacttt tttcgaacac cattggcaga acttcttgtt atttcgtatt
    12481 tttttgtgcg tcagctaaag cttgatcgac atcttcgttt ccgacagtta cttggttcac
    12541 tgcttttccg atcacacctg agcttgtgat atctcccatt ttcgtgaaat tcttttcccc
    12601 gactagtcca aagacttgta cttcatcaaa agcactagca atctcagatg gcagctcgcc
    12661 aaaagacttg atgacttcat tttctttgta accatccagt tcgactactt gtttattcac
    12721 tggttgagca ccaccaggag acatcaagac ccacttttcc atgtttttcg gttgagataa
    12781 gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg tctagaccag aagaaatcgt
    12841 caatccagat actgtgccat agacagcttg atctttttgt gttgggatag caaaaccgat
    12901 attttctgag ttccccgctt cataaacaga aggcaaaatg taagttgaat agattgccat
    12961 tggtgctgtt ccattcatga aagcatcttt gatttctgtc acatcattag agcctggcat
    13021 cgtgtattgg gataagtttt gataaaacga caatgcttct ctcatctttt ccgtatcgat
    13081 cgttacctcg ccttcatcat ccaaaacatt tgctccattt gacaaagcaa attgagagaa
    13141 cgcttgctca ctcattgttc cttcagcagt aggcatagca attccatatt gtttattctc
    13201 tttatctgtg aaatgttgag caatttttga aatatcatcc caattttctg gttctgaaaa
    13261 accagcttct gataattttt ctttgtcata ccaaatacct tgaacccagc cgctaattgg
    13321 tgcagcaata tagctttttc catcttctga acgaacaagg tttttggctc cgtcataata
    13381 attatcttct ccagcttcct cgataacgtt ttggactgcc gtctgatcaa tcagttcatc
    13441 tttatccatc actttagcaa aatcctgact gacttccatt acttctggta atttaccgga
    13501 acgagctaac gtaacgattt ttgtattgaa cgctgattct tctaccggca cttgcttgat
    13561 tttgatgtct ggattttctt tttcaaaatc agcaaccagt tcattgatca cacttaatcg
    13621 ttcctgttcg acagaagagt gcatgaattg aatcgttgtc tgttgtgatt cgttagagct
    13681 ttgactttca ccacaagcag taagaagaaa tccagtagct agcagacagg tagtaacaat
    13741 aatttttttc atttttttcc acctcttatt ttattatttt cagccacata tattcatacg
    13801 gctggaatat caactttcgt tcttgactgc aatggataat ttgtccagaa aacaggtcta
    13861 ggtaatctcc ttttggtaaa agataacctg ctgattgatt ggaaaaatta tggataacta
    13921 agactttctc ttccttcgct tcgcgaataa aactgataaa agaatctgct gagtccagta
    13981 agtaaatcaa actattcgga tggaaggctt tttctttttt tcgaatagat agcatctggg
    14041 tcaattcacc atatactgct gttcttaatg gatctgtctg caactctttc tcaatatctg
    14101 ctaacaaaaa tttttgccga ttgatttttc gattctctcc tgttttttct acgcctttgt
    14161 aatcatttcg acttcccagc acactttgaa tataaattgc cggcacgcct ggtaacgtta
    14221 ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag
    14281 aacttaacgc atctagatac gtagcgttta tctcatatgg actggtcgtt ccatctgggt
    14341 ttttcttgta ctttactcgg gcaccctctt tcttcagctg ttccactaat tcaaggatcg
    14401 ttttttccgg cagaatacct cgtaccggat tcaatccgat accgtcatgg gaggctaaaa
    14461 aattaaaaaa agttgttttt ttcgatgcga aatcgatggt ttttaaccat tctttcaact
    14521 tacttgtatt acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa aactgataga
    14581 ccatctgtgc ttcattctcg ccattcccaa aataactgat attatctttg tgtggcacat
    14641 ttgtctcagt gatcaatacc gttcctggag ctgcttcatc agcgattgca cggaataatt
    14701 ggatgatttt atgcgtttct ggcaggtgta tacaagaagt tcctagttct ttccataaaa
    14761 atcccacagc gtccaagcga ataaatgaag cgcctttttc taagtaaaac aaaagaatat
    14821 cgatcatttt caataaaact tctggatttt taaaattcaa gtctacttga tcttcgctaa
    14881 aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta agtaacgggc
    14941 tggtccttgg tctagtcacg ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat
    15001 ctttgtacgt atcattcaag tttaaatatt cctgaaacca agtactttcg gcagacatat
    15061 gattgcagac aaagtcgaac attaaccgtg tttcgcttct catttcttct atatcttccc
    15121 atgtgcccaa acggtcgttg attgcctgat aatttacaac ggagaaacca tcatcagatg
    15181 tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa tacttttgat
    15241 aaaatttctt aaatgtacgc aaagttggtt gctgctcttc aaaaaattga tccccataag
    15301 taatcaagaa aatatcttct tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct
    15361 gataaacact gactttttgt tcaatcatag aggcggtttt ctcagccaaa tctgagtcgc
    15421 gataaatcgc ttgtaaaagt tttgtataca tcggattccc ctttcttttt tgggagcgtt
    15481 cccaacgatt tgatcatatt atggaaaacg cttactgtca atagcatttt tttaaataat
    15541 tttgatatac acaaaaatac aaataagaaa gcttatgtat caataattaa tagcttctta
    15601 aaaaaatatg gtctctcatg atcatgcaag ctattcctaa attttcaaaa tcatagacaa
    15661 aaaagaatgt ctattgtact atggaattat tctaaaacgc tcccaaaaag gaggtgcata
    15721 taaaattgaa acctactatt tatgacattg cgaaaaaagc taatgtctca aaatcaactg
    15781 tctccagagt attaaataac cagccaaata tctctgataa agcaaaagca agtgtacttc
    15841 aagcaatcaa agaattgaat tatgaaccga gcaaaattgc aagaggactg tcaggaaatg
    15901 gttttgacgc gattctagca ttatctcatc gtacttctca tacgacctat ggcaatcctt
    15961 ttttttcaga tgttattcaa tccatttctg ccgtttctga acaaaataac ttcgacttga
    16021 ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa aagatccaag
    16081 aaaaaatgat ccgaggaatt attattttaa gctctacgat ggatgaaaca cttctttttg
    16141 aattggatca atacaatatt cctattgtgg taatcggaaa agtcggaaac gactacacga
    16201 acatctactc agtggacacg gataatttcc aggatagtta cgatttggtg gaaacactga
    16261 tcaagaatgg acaccgtcat atcggctgtc tccatgctcc gacagaagtt tatgtatcca
    16321 atgatcgtct caatggttac cgtcaatgct tatttgatca ccacctagat atccgtaatg
    16381 aatggattat agacggcggc tttcgcttag aagatagcat ggcagcaatg aatgatttaa
    16441 tgaagctacg agaaagacca actgcgattt tttctaccga tgcattaaaa tcattgagta
    16501 tttacaaagt ggcagaacaa tctggaaagc agatacctga tgatctttcg atcgcaggat
    16561 tcagtgatcc cttattgtcc tccctatttt cgccacagtt gactcggata gacattccga
    16621 caaatcagtt aggaatcaaa gccacagaac ttttattcca actaatccac caccaatacc
    16681 ctgaagaaaa aagaatcatt ctttcaaccg agattactct cacagattcg atccaacaac
    16741 aagaaatgta gaacagaatt ataaaagaac agaaacactg aggataagtc agttactctg
    16801 ttcttttttc aatcactcat ccctcgttac cccctcccgg tattttagcg aatttttctt
    16861 attttccatc tgttttagat agctgtggat aactataatg tttgtgttaa actatttttt
    16921 ccacagggaa gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt
    16981 tttaaacaca tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca
    17041 taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata
    17101 gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa
    17161 aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct
    17221 taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa
    17281 ttattttaga caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa
    17341 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
    17401 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacggtaa
    17461 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
    17521 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
    17581 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
    17641 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
    17701 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
    17761 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
    17821 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
    17881 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
    17941 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
    18001 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
    18061 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
    18121 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
    18181 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
    18241 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
    18301 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
    18361 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
    18421 aattcatttg tttcaggatg acgtacttct ttacgagtgt aacgtcccac taaacgatct
    18481 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
    18541 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
    18601 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
    18661 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
    18721 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
    18781 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
    18841 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
    18901 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
    18961 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
    19021 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
    19081 agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
    19141 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
    19201 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
    19261 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
    19321 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
    19381 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
    19441 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
    19501 tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc
    19561 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
    19621 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
    19681 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
    19741 cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
    19801 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
    19861 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
    19921 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
    19981 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
    20041 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
    20101 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
    20161 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
    20221 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
    20281 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
    20341 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
    20401 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
    20461 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
    20521 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
    20581 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
    20641 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
    20701 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
    20761 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
    20821 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc gcgttcaggt
    20881 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
    20941 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
    21001 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
    21061 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
    21121 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
    21181 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
    21241 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
    21301 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
    21361 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
    21421 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
    21481 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
    21541 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
    21601 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
    21661 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
    21721 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
    21781 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
    21841 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
    21901 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
    21961 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
    22021 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
    22081 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
    22141 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
    22201 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
    22261 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
    22321 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
    22381 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
    22441 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
    22501 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
    22561 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
    22621 gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca
    22681 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
    22741 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
    22801 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
    22861 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
    22921 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
    22981 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
    23041 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
    23101 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
    23161 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
    23221 gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
    23281 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
    23341 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
    23401 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
    23461 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
    23521 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
    23581 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
    23641 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
    23701 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
    23761 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
    23821 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
    23881 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
    23941 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
    24001 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
    24061 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
    24121 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
    24181 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
    24241 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
    24301 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
    24361 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
    24421 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
    24481 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
    24541 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
    24601 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
    24661 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
    24721 tcaaaaagac tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca
    24781 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
    24841 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
    24901 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
    24961 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
    25021 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
    25081 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
    25141 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
    25201 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
    25261 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
    25321 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
    25381 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
    25441 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
    25501 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
    25561 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
    25621 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
    25681 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
    25741 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
    25801 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
    25861 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
    25921 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
    25981 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
    26041 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
    26101 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
    26161 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
    26221 gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag
    26281 agtccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
    26341 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
    26401 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
    26461 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
    26521 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
    26581 atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt
    26641 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
    26701 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
    26761 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
    26821 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
    26881 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
    26941 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
    27001 ctggctcctt gcttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
    27061 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
    27121 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
    27181 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
    27241 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
    27301 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    27361 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    27421 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    27481 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    27541 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    27601 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    27661 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    27721 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    27781 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    27841 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    27901 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    27961 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    28021 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    28081 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    28141 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    28201 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    28261 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    28321 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    28381 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    28441 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    28501 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact
    28561 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    28621 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    28681 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    28741 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    28801 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    28861 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    28921 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    28981 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    29041 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    29101 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    29161 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    29221 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    29281 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    29341 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    29401 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    29461 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    29521 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    29581 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    29641 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    29701 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    29761 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    29821 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    29881 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    29941 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    30001 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    30061 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    30121 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    30181 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    30241 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    30301 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    30361 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    30421 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    30481 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    30541 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    30601 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    30661 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    30721 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    30781 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    30841 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    30901 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    30961 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    31021 gtgttaacga tgaatacgcc acttcaaatc atgaaacaga agatttttcg gatgcgaata
    31081 tctcttggaa ttttggaaat gagatgactg atttgacaat cagtgctcct tttcaaggaa
    31141 caatccttgc gcctaatgct actatccgca ttactcaaaa ccaagacgga acgattatag
    31201 gaacaaatgt gattttagat gcagctacca atcgttggga tcctaacgaa atatttatca
    31261 gcgacaatgg tacagatacg acagacacaa gcgacagtac agatacatct accacaagtg
    31321 atagctcaga ttcatca
//
LOCUS       DACEPW010000012        30750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEPW010000012 DACEPW010000000
VERSION     DACEPW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.012"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1428)
                     /gene="aspS"
                     /locus_tag="IW819_000432"
     CDS             complement(<1..1428)
                     /gene="aspS"
                     /locus_tag="IW819_000432"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1243563.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYD"
     gene            complement(1443..2735)
                     /locus_tag="IW819_000433"
     CDS             complement(1443..2735)
                     /locus_tag="IW819_000433"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1243564.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            3129..4076
                     /locus_tag="IW819_000434"
     CDS             3129..4076
                     /locus_tag="IW819_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1243565.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(4165..5424)
                     /locus_tag="IW819_000435"
     CDS             complement(4165..5424)
                     /locus_tag="IW819_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1243566.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(5595..6041)
                     /locus_tag="IW819_000436"
     CDS             complement(5595..6041)
                     /locus_tag="IW819_000436"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1243567.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(6052..8265)
                     /locus_tag="IW819_000437"
     CDS             complement(6052..8265)
                     /locus_tag="IW819_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1243568.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(8498..9262)
                     /locus_tag="IW819_000438"
     CDS             complement(8498..9262)
                     /locus_tag="IW819_000438"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1243569.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(9263..10210)
                     /gene="prmA"
                     /locus_tag="IW819_000439"
     CDS             complement(9263..10210)
                     /gene="prmA"
                     /locus_tag="IW819_000439"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1243570.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(10223..10711)
                     /locus_tag="IW819_000440"
     CDS             complement(10223..10711)
                     /locus_tag="IW819_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1243571.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(10677..11348)
                     /locus_tag="IW819_000441"
     CDS             complement(10677..11348)
                     /locus_tag="IW819_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1243572.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            11486..12772
                     /locus_tag="IW819_000442"
     CDS             11486..12772
                     /locus_tag="IW819_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1243573.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            12844..13038
                     /gene="ssrS"
                     /locus_tag="IW819_000443"
     ncRNA           12844..13038
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW819_000443"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            13116..13400
                     /locus_tag="IW819_000444"
     CDS             13116..13400
                     /locus_tag="IW819_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243574.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            13473..13952
                     /locus_tag="IW819_000445"
     CDS             13473..13952
                     /locus_tag="IW819_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1243575.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            14174..14317
                     /locus_tag="IW819_000446"
     CDS             14174..14317
                     /locus_tag="IW819_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1243576.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            14895..16478
                     /locus_tag="IW819_000447"
     CDS             14895..16478
                     /locus_tag="IW819_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1243577.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16915..18099)
                     /locus_tag="IW819_000448"
     CDS             complement(16915..18099)
                     /locus_tag="IW819_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1243578.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(18124..19131)
                     /locus_tag="IW819_000449"
     CDS             complement(18124..19131)
                     /locus_tag="IW819_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1243579.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(19303..19662)
                     /locus_tag="IW819_000450"
     CDS             complement(19303..19662)
                     /locus_tag="IW819_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243580.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(19634..20005)
                     /locus_tag="IW819_000451"
     CDS             complement(19634..20005)
                     /locus_tag="IW819_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1243581.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(20022..20324)
                     /locus_tag="IW819_000452"
     CDS             complement(20022..20324)
                     /locus_tag="IW819_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243582.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(20341..20811)
                     /locus_tag="IW819_000453"
     CDS             complement(20341..20811)
                     /locus_tag="IW819_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1243583.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(20786..21064)
                     /locus_tag="IW819_000454"
     CDS             complement(20786..21064)
                     /locus_tag="IW819_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1243584.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(21061..22110)
                     /locus_tag="IW819_000455"
     CDS             complement(21061..22110)
                     /locus_tag="IW819_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1243585.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(22067..22996)
                     /gene="cpaF"
                     /locus_tag="IW819_000456"
     CDS             complement(22067..22996)
                     /gene="cpaF"
                     /locus_tag="IW819_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1243586.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            23128..24882
                     /gene="ade"
                     /locus_tag="IW819_000457"
     CDS             23128..24882
                     /gene="ade"
                     /locus_tag="IW819_000457"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1243587.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(24980..25642)
                     /gene="trmB"
                     /locus_tag="IW819_000458"
     CDS             complement(24980..25642)
                     /gene="trmB"
                     /locus_tag="IW819_000458"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1243588.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(25712..26491)
                     /locus_tag="IW819_000459"
     CDS             complement(25712..26491)
                     /locus_tag="IW819_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1243589.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(26698..27909)
                     /locus_tag="IW819_000460"
     CDS             complement(26698..27909)
                     /locus_tag="IW819_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1243590.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(27902..28639)
                     /locus_tag="IW819_000461"
     CDS             complement(27902..28639)
                     /locus_tag="IW819_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1243591.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            28777..29211
                     /locus_tag="IW819_000462"
     CDS             28777..29211
                     /locus_tag="IW819_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1243592.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            29204..29548
                     /locus_tag="IW819_000463"
     CDS             29204..29548
                     /locus_tag="IW819_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1243593.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            29728..30738
                     /locus_tag="IW819_000464"
     CDS             29728..30738
                     /locus_tag="IW819_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1243594.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPELEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         9181 a         6445 c         5499 g         9625 t
ORIGIN      
        1 atcataagct tctgcataga cttttgcagg gtcttcagcc aataacggga tatcttcttc
       61 ttttggcatc gtgaatggat ggtgagcaga gacatatcga ccttcttctt ctgaaaattc
      121 aaattgtggc caatcagtta cccataagaa attgaatttc gattcatcaa ttagtcctaa
      181 ttcttttcct aaacgtgtcc ggatagcacc taatgcagca gcgacgattt cacttttatc
      241 tgcgccaaac atcagcaagt cacctggttt tgcatctgtt gctttaatga ttgcttctgt
      301 tgcttctccc atgaatttag caattggacc tttcaatccg tcttcttcta ctttcagcca
      361 tgcaagcccc ttagctccga attgtcctac atattgacct aactgatcca tgtcttttcg
      421 tgagtagcga tctgcagcgc ctttagcatt caatgctttc acgacaccgc cgttttcaag
      481 agccatttgg aagactttga attctacttc tttgactgta tcgcttagat cgatcaattc
      541 catatcaaaa cgtgtatccg gtttgtcgct gccgtagcga gccattgctt catcataggt
      601 catacgagga aatggcaatg tgacttcgat accgcgaact tctttcatta ccttcgcaat
      661 caatccctct gtgtaagttt ggatttcttc tgctgttaag aacgttgttt caatatctac
      721 ttgcgtaaat tcaggctgac ggtctccacg taaatcttca tcgcggaaac aacgaacgat
      781 ttgataataa cggtcgaatc cagcattcat cagcaattgt ttaaacaatt gaggagattg
      841 cggcaacgca tagaaatgtc cagcatggac acgtgaagga actaggtagt ctcttgcgcc
      901 ttctggtgta gatttcccta ggtaaggtgt ttcgatatcg agaaagtcat tatcatctaa
      961 ataatgacga atcgcttttg ttgtcgcatt tcttaatttg atgtttttag tcatttttgg
     1021 acgacgcaga tccaaataac gatatttcaa acgtatttcg tcactgattg cttgatcatc
     1081 ttcaatttga aatggcgggg tttttgcttg attcaaaatc acgatttcac ttgcaatcac
     1141 ttcgaattca ccagtggcca ttttaggatt tactgcattt tctccgcgtc ttctaacttc
     1201 cccagtcact tcgatcacgt attcactgcg acatttatcc gcgatttccc atgcttcttt
     1261 gttgatttca ggattaaaga cgatttgaac gattccttca cgatcacgta ggtcgataaa
     1321 gattactccc ccaaggtctc gtcttttttg tacccatcct tttaatgtaa ctgtttgttc
     1381 taattgttgt tcggaaacta atccgcaata gatcgttctt tgtgccattt atttttttcc
     1441 tcctactcga ataaagtcat ttcatcatag acttcatcaa aatgttcata tatctcttct
     1501 aatggaaaac ttttttcttt tcggcttgcc attgctttga cattaatcgt ttgactagca
     1561 agctcatctt caccgatcac caatactaat ttggcattcg ctttatctgc tgtcttaaat
     1621 tgcgcttttg ctttacggtt catgaagtcg cggtcagctg aaaaaccaaa gtttcgaatc
     1681 gcttggacca ctttcaatgc ttcgatattt gtttcgtctc cgagtgcgac cacgtaagca
     1741 tccaactcat taaatgcagg gataactact tcctctgcct ccatagtcaa gagtaatcgc
     1801 tcgataccca ttgcaaatcc gaagccaggt gttgaaggac cacctagttc ttccactaga
     1861 ttgtcataac gtccgcctgc gcaaatcgtt gcttgtgccc ccatttttgg tgcgtcactc
     1921 atgatttcaa aaatcgtatg tgtgtaataa tctagtccgc gaaccatatt actgtcgatt
     1981 tcataaggaa cagccaatgc atccagcatg gaaacaacgg tatcaaaatg tttttgagca
     2041 ggctcgctta gataatctaa gatagatgga gcttctgcca caaatttttg atctcgtttg
     2101 tctttactgt ccaatacgcg taatggattt tcatgaaggc ggcgtttaga gtcttcactc
     2161 agctctgatt caaaaggtaa aagatagtcg atcaatgctt gacgataggc ttgacgcgtt
     2221 tctttatcac ctaatgaatt gatgactagc cggatttgcg tgatccccaa ctgtttgaag
     2281 aaatcaagtg ccatcaccat gctctccaca tcagttgcag ggttttctga tccaaatgct
     2341 tctactccga tttgatggaa ttgacgtaac cgtccttttt gagggcgttc gtagcgaaac
     2401 attggtccca tataaaaagt tttgaacggt ttcgcatact ctgggccgaa caatttatgc
     2461 tcaacaaaag aacgaacgat tggtgctgta ccttctggtc gaagcgtcac atggcgttcc
     2521 cctttgtcat aaaaatcata catctctttt gaaacgatat ccgttgtatc ccctacactt
     2581 cgggatatca cttcatagtg ttcaaaaatc ggtgtacgca tttcattata ctgataatct
     2641 ctgaataata aacgtgctgt ttcttcgaca aactgccatt tttctgattc gcctggcaaa
     2701 atatcatttg ttccttttgg tctttggaaa ctcatagttt tctctccctt tcttagctat
     2761 gtaaataaaa aaatcaccct tgttcatcat gaacaagggt gaatgaaatt catacacggt
     2821 accacctaat tttgaaagat ttctctttcc tcttacgaat aacgctcgta cgcgggacgg
     2881 cttttcactc gtccatctcc agagtgtctt taggctaatg aaactttaga tcattttcag
     2941 cctcgatgat cctctcttgt aaagtttcaa aaacttactc gctctttcat tgacattcga
     3001 tattacttat aacctactaa aaaccagtct caaagtcaag agatatctga ctatttcccc
     3061 tcacaaaaac gttttagttc agctttttgg tattttgttt tagactagac aaaaaggagg
     3121 aatcagctat ggcagaaata agaatcggac attcacaagt ctacgcagaa cagcttggat
     3181 taggagcaaa tgccgttgga ggacataatc tttttgacgg tttggaagat gagacaggaa
     3241 aacaagtagt tcgtacagct ttgaatagcg ggatcaattt gatcgatacc gcttatgctt
     3301 acggaaacgg ccgttccgaa gaactgattg gcgaggtact gaaggaaaaa gaatatgatc
     3361 gctcacgtgt cgtcatcgct acaaaagctg ctcatgtacc aaataaaggg cgaacatttg
     3421 ataattctcc tgagtttctc aaacaatcgg tggaagatgc actaaaacgt ctgcaaactg
     3481 attacatcga cattttttat atccactttc cggatgaaag cacaccaaaa aatgaatcag
     3541 ttgctacatt gcacgaatta aaagaagcag gaaaaatccg agcagtcggt gtgtcaaact
     3601 tcacgttaga acaattgaaa gaagcgaatg cagatggata tgtcgatgta gtagaagata
     3661 aatacagttt gatccatcgg caggctgaaa aagaattatt cccttatctt gaaaaaaaca
     3721 aaatcagttt tgttccctat tttcctcttg cttccggttt gctgacagga aaatatgaac
     3781 ttggtgagga aaaacaattt ggtgaaggag atcctcgaaa aagaaatcca gatttccaag
     3841 gagaacgttt cagagaaatt ttaaccgctg tcgatgtact tcgccccatt gctaaaagat
     3901 accaagccac tcctgcgcaa cttgttttag cttggtacat gaagaatcca cgagtgtctg
     3961 tagttatacc tggagctaag cgtccagagc aagtatctga caatgtccaa gctttagatt
     4021 tgcatttatc caatgaagat tatcaaacca ttgatgaagc atttcgtgga ttttgatcaa
     4081 ttgaccttat caatttatag taatatgaaa aaaggaagat cagcactgtt tgatcttcct
     4141 ttttttcgat aagtttatgc taatctaatt cttgtaaagt agcgctcggc tattcggata
     4201 agtcggaaac tgattgaatg gaatcgtcca atcttgttca ggtacagaga actcaaactt
     4261 atttactagc tgatcggaaa acacacgtag actatgaaga gtgatccatt ctcctgcaca
     4321 gcgatgcatt tgtcgaaaat ttcccccgcc ttgtgcaatc atctcatatt cttctttata
     4381 ggaaatatct tttgctttcc ctacataccg tttgatcata aaggaatctg gcgcttcaac
     4441 tgttcgttca tcatggtctg ttccatataa atctaaaata acccaactgc cttctggtat
     4501 acgataacca tctacttcta cttctttcag cgaaatcgca ggtagcattg ggaagaaagg
     4561 ataatagcgt ctcatttctt gaataaacgg atcttgtaac gtcgaaaagt cattttttaa
     4621 ttgctgatac aaatctggtc tactgaaaag tgcatgtccc atcaatgccg cccacacagt
     4681 tagagcaacc gttggacgga tgatgttcaa taattcaaca gctgccactt ccagaggtaa
     4741 aagctgacca tctaaatctg tggcattggc aaaagcatat aatgccacat tttcttttcc
     4801 agccacagga ttttgtcgag cttcttcaat caagccttgc gcccattttt ctgatttttt
     4861 gcgattctcg acacctttta tgtgatcaat cggagaagta aacgttccgc tgatcataga
     4921 gatttgatat tctgctaatt tttcgacctc atctttagaa atcgctgata aattgatacc
     4981 tgcccactcg caaatagatg taaagaaaac catcttactt aaatcaaata gttcaaattg
     5041 accatgctgt gcttcaagtg cttgtgtcag attcttatct aagatacgat gataatcttc
     5101 cattctttcc ggtgtcatta gatccataaa aatatttttc cgatggtgat gagcagctcc
     5161 atccaaagtc tgtactccac cttgaccaaa aagtgtcttc aatactaatt ttggcatagc
     5221 accttttcgt ttgaaattcc ttggatcata aaatactttt gctgctgatg atccgtagat
     5281 cgtgatagct tctttattga aaatctttgc tttgacgacg ggagcgtccg cttcatggcg
     5341 taactcctcc aacatgttgt atcctttttg atacaatttt tttaaatctg tgattttgat
     5401 atctactact ggtacttctt tcatcttcag caacctccta taaaaaataa cttgtataat
     5461 ttaagcttaa ctttttttcc gactgctgac aaaagaagcg ctttttgcag acaaaaaagc
     5521 cacttaagtt agagtttatc agtctaactt aagcgacgtg tttcatagtt tttactagtg
     5581 aaaaccagat tactttattt gtttttagaa tcaataatga tcgttacagg tccatcattg
     5641 acgagagaaa ccgacatatc tgctccgaat tgtccggtag cgactgtgat gccgcgactt
     5701 ctgattcctt cattgaatga ttcgtataaa ggaatagctg tttctggtcg tgcggcttcc
     5761 acaaagcttg gtcgatttcc ttttttcgta tccgcaaaca gtgtgaattg agagatactt
     5821 aaaatttgtc ctcccacatc ttctacacta atattcattt ttccttgttc atcttcaaaa
     5881 atacgcattt gtgcaatttt ttttaccaga tagtccacat ctttttgcgt atcggtttca
     5941 tgaatgccta ataaaacaag taggccgtag ccaatttttc caatttcttg ctgatcgact
     6001 gacacagcag ctttacttac ccgttgaaca actgctctca tatctgtcct cctatccatt
     6061 cgtccggcgt acactgtaga catcaggtac tgctttgatt ttatccacga tactctttag
     6121 gtgggataaa ttctggatac caaccgtcaa atggatcgtc gccattttat ctttattcgt
     6181 ccgtgcttca acgctgttga gatttttcgt tagcgcattg actgtttgaa gtacatcatt
     6241 caataagcct gaacgattat agccgtaaat ttccaaatct gcatcatact cttttcgtgt
     6301 attcgatgta tcttcccatt cgacttcaat caaacgttct gctacatttg gtttgtcagg
     6361 ctgaacattc ggacaatctc gacgatgaat ggatatccct ctgcctttag tgatataacc
     6421 aacgatatca tcaccaggaa tcggattaca gcagcgactg atacgaatta gtaagttttc
     6481 taccccttga atgacaacgc caccttcatg acgtactttc attttgtcag gttctttttt
     6541 cggctgattc atgatttctt ctgcttcttg cttttgctgc tcgatttttt gttctttacg
     6601 ttctttttca gtcagacgat tggccatcgt caaggggcta acttctccat accctacagc
     6661 tgcatagaga tcatcttctg tttgataatt aaaacgatcg agtgcttctt gcagtttgtt
     6721 cttcgtcaaa atatctttag gcgtaaatcc tagatctgta atacatttga ccacggattc
     6781 gtggcctttg atcacatttt cttctcgatc ttgggctttg aagaaacgtt tgatcttatt
     6841 tcttgctttg ctagtagcaa ctaatttcaa ccagtcgcga cttgggccaa atgaatttgg
     6901 agaagtcatg atctcaatga tatctccgtt tttcaatttg taatcaagct gcaccatttt
     6961 gccatttact tttgcaccag tggttttgtt accgatatct gtatggatac tgtatgcaaa
     7021 gtccaatggt ccggatcctt ttggtaactc agtaacgtct ccttttggtg tgaagacgta
     7081 gactttatca ctaaagatat ctccttttac gccttccata aattcagaag catcatagct
     7141 ttcgtcttga agttcgagta tctcatggaa ccagcttaat tgtttcgtca taccatctgg
     7201 ttctactttt tcattttttc cttctttata ggcccagtgt gcagcaaccc cgaattcagc
     7261 gatttcatgc atttcctgtg ttcggatttg aatttctacc ggatttcctg ccggaccgat
     7321 cacggtagta tgcaaagatt ggtacatatt agcttttggc atggcaatat aatccttaaa
     7381 tctgccgggc attggtttcc attttgtgtg gattgctcct aatacagcat aacaatcctt
     7441 gattgaatct acgatcactc gaatagctag caagtcataa atttcgttga actgcttttt
     7501 ctgatctttc attttacgat agattgaata aatgtgtttt ggccgtccat agatttctgc
     7561 aaaaatcccc agctcttctg tcgctattcg aatatcttca acagtaccac ttacgtattt
     7621 ttcacgttct tctctcttcg tctgcatcaa gtggacgata cggtaatact gttttggatt
     7681 tagataacga agtgctgtat cttctaattc ccatttgatc cggctgatcc ctagacgatg
     7741 tgcaagaggc gcatagattt ctaaagtttc ctgagcgatc cttctttgct tatcttctcg
     7801 caggtgcttc aacgtacgca tgttatgtag tctatcagct aatttgacca tgataactcg
     7861 taaatcttga gccattgcta gcagcatctt tcggtggttt tctgctagct gctcttcgtg
     7921 ggatttatat tttattttcc ctaatttggt tacgccgtcc actaacatag caacgtcatc
     7981 gccaaattct tctctcagat cttctaaagt gatttctgta tcttcaacaa catcatgtaa
     8041 gaagcctgta gccactgtat ggggatccat ttttaaatct gctaatatac ctgcgacttg
     8101 gataggatga ataatgtagg gttcgcctga ttttctaaac tgtccgtcat gtgcagcagt
     8161 cgcgtattcg catgctttct caacaaatgc tacatgttcg ggacccatgt attgactaac
     8221 gagctttatg acaccgggtc ccgttaaaat ttcttctttt gtcacaggaa tcgatccccc
     8281 tttctttttt cggtaaaacg ggaatcggga caaatatctg tttgataatt gtcccaaaat
     8341 ccgtgcagtt atacgttttt ttacggaaat gttcgctttt taaaagcttt ttcaaacgat
     8401 ttttcgtatc cattgtataa ataattgttt ctattataca aattgttgac tattttttca
     8461 attctttctg tcgatactct tttattaatg aagaacgtta gctgaaagaa ggattcatta
     8521 attcccattg atagctagca gcacttaata aataaagagg tgctgtttct gtacgaagga
     8581 ttcttggtcc taatccacaa agtatccctt tgtgttctac aaaataatcg acttcatcgc
     8641 gggataatcc accttctggt ccaaagacag ctaagattcg actatctggt tttgtttcac
     8701 taagaatttt ggctgcattt gatttttcgc cttgctttgc tgattcctca taagcaacca
     8761 agaccacgtc ataatcatct agccgctcaa ataaatcttg tttcgtttcc aataactcta
     8821 ctgatggcac gatctgccga tgggattgtt ccgctgcctc ttgagcaatc ttttcaagcc
     8881 gttgttgtcg tttcatacgt ttttttgcat cccatttaac gacagaggct gatgccggga
     8941 aaccaagaaa agcttgcgct cctaattcag tggatttctg aacgatccaa tccagctttt
     9001 cccctttagg atagccactc gctatggtaa tcgaacttgg cagttccttt tgattttctt
     9061 cttttttaat ttctttcaga taaacagcct cttcagtaat ctccgtgatt tctgcaacaa
     9121 tggctatttg atcattgaat gccaaaaaaa cttcatcctc gggttccatc ctcataaccc
     9181 gtacaatgtg gtgatagttt tccccttcta aaacaaaata gtcttttgat tcatatgatt
     9241 cttttaaaaa ataccgctgc atctattcca cctctggttt tttcaaaata attgcatacc
     9301 agtctttttg ctggaaaatt tgatccacga taaacccttg ttctaacatg gcatccagaa
     9361 ccatttgctt cttctcttca atgattcctg aaacgatcag cgtgcctgtt tgcttcagta
     9421 aacgccaagc atctgggatc atcaataaga tgatatctgc taaaatgttc gcaacgatta
     9481 catcactttc aatctcgatt cctgtcaaaa gatcgttagc tgatacatgg acatctttgg
     9541 ctatttcatt catatccata ttttctttcg ccgaacgaac ggctacttcg tctaaatcaa
     9601 aggcatagac atcttttgca ccaagatatt ttgctgcaat acttagcacg cccgacccag
     9661 ttcccacatc tagcaatgtt tcaccaccac gcaagactgt ttctagtgct gtcaatgtca
     9721 aggaagtcgt gggatgagtg cctgtgccaa atgccatacc aggatctagt gtgataattt
     9781 tttctagagg atcttctgtc tggtagtttt cccagcttgg cgcaaccgtc agcattcgcg
     9841 taatacgcac cggatgatag tatttcttcc aggctgttgc ccagttgctt tcctctactt
     9901 cactaactgt tactttattt tttccaatag ctagaccaaa ctcaggtaat tgtgtgatac
     9961 gatctttgat aaaaggcaag atttccggta agaaagtcgt ttccggaaaa taagccatga
    10021 ctagtgcgcc ttctttcagc gatgtaaaat catttttatc taaaatctca ccatattgat
    10081 ccgactgaaa atgttcaaca tctaaagaat cttcaatcgc gacaccactc gcccctgcct
    10141 ccatcaaaat attagcaact gcttcgactg cttcactttc agtttctact tttacttcat
    10201 tccatttcat atctgtcact ccttaataac ttgggtacgg taaataagta tctgcttcat
    10261 tttgctgttt tacttctact aattctgcta gttgttgatt atcccagtga agctcgaata
    10321 cagcttcgtc atcgtcttca tccaaaaaat caagcaaatc gctttgccct tgagctaata
    10381 cttcatttag atattccact aaagaatcag ctactgattt tgccaaccct ttttttcctt
    10441 cataagggat tactgctaga taattgcgtt catcaaaaac agatttctga ggattaaaaa
    10501 gaagtacgcc gtcttcaaat tcgataattg gttcttgcga aacgatccct tctgcatcat
    10561 caatctcaaa ttgtgcttta ttttctgcaa acaagcgaac aacgatctcg atcgtatgat
    10621 tttttgtatc ccaatctaaa gctacatcat agtctgtcac ttgtttttca attatttcat
    10681 ctaaatatgt taatagatcc ttctttttca ttttctttcc tccaacccca atctttctcg
    10741 atttggtttt ttctttgtaa agagatataa ggatttcccg acacaacaaa acgcaacggc
    10801 ttttctgtcc atacaccttt gtttgggata ccgattctag gcaaagcatt gatctttttt
    10861 ggtgtctttt ttttctcaaa aactaagcgt aacgaactat ctgctataga ctgaccataa
    10921 agttcttgag gaattgctaa tgcttccact aattttcccg gaccgttgct gatatctgga
    10981 ccagtttttc cgccacgatt tttactcatt tgatcaatca ttgcggcggg ttcgattgca
    11041 cgtatcatta ctccttgagg aatcccttct ggctgagtaa tgatgttcaa aatccgatga
    11101 gtatgcatcg tgtaaaggta gatcgtacct ggtttttcat acattgcccg cacacgcggt
    11161 gttcttctta aaccatagct atgggcggcc tcgtcttctg gacctaggta tgcttccgcg
    11221 tctacaatat atccagctaa tcttccttct ggtgtgtcat gctccaaata catccccaat
    11281 aaatcccgtg ccacttctgg cgtcgtttta ttccaaaaaa attcatctat cttttgttta
    11341 tccgtcattt ttctcaccta ctttatcata cacaatatta gttggataag ctatgaaaac
    11401 aaaacaacag aaggctgttc tttctaggca tcttgctaaa tatgatataa ttaagggacg
    11461 taaaaacaag aaggaggaaa atcctatgca acagccattg gcttatcgca tgagacctcg
    11521 aacgatcgat gaagtcgttg gtcagcagca tctagtcggg gaaggaaaaa tcatacgccg
    11581 catggtcgat gctcgcatgc tctcttccat gattctatat ggacctcctg gtactggaaa
    11641 aacgagtatc gcaagtgcga tcgccggctc tactaattat gcatttcgca tgctaaacgc
    11701 cgctacagat tcaaaaaagg atctacaagt tgtagcggaa gaagcaaaaa tgagtggtac
    11761 agtgatcctc ttattggatg aagtacaccg attagataaa acaaaacagg attttctgct
    11821 tcctcattta gaaagtggcc gaatcatctt gatcggagcg actactgaga atccttatat
    11881 taccatcaat ccagcgatcc gcagccgtac acagatcttt gaagtcaaac cattaacaga
    11941 acaagacatc cagttggcag tcgaacatgc tttaagggat aaagaacgcg gccttggaca
    12001 acaagcaatc cagttagacg aagatgctct tcttcacctt tcgcgtgcaa cgaacggcga
    12061 tttgcggagt gcattgaatg ggctggaact ggctacacta tcgactcctt cagataaaga
    12121 aggaaggatt catcttacac tgtcgatcat tgaagaatgt gtccaaagaa aagctcttac
    12181 ccatgacaaa aatggagatg ctcattatga cgttatttcg gcttttcaaa aatcgattcg
    12241 tggaagtgat gtggatgctg cacttcatta tctcgcccgt ttagttgagg caggtgatct
    12301 ggcaagtatt tgccggcgtc tgatggttat cggttatgaa gatataggtt taggtaatcc
    12361 tgctgctgcg gctcgtacag tgaacgctgt attagctgct gaaaaattgg gattgcctga
    12421 ggcacgtatc ccactagcag acgtcgtcgt cgatctttgc ttgtcgccaa aatccaattc
    12481 tgcctacatg gcattagatg cagcacttgc tgatattcga gagggcaaag ctggagatgt
    12541 tcctgatcat ttgagagata gccattacaa aggtgcgaaa tcactcaacc gtggcgtggg
    12601 ttatcagtat cctcatcatt ttgatcaggc ttgggtcaat cagcaatatt taccagataa
    12661 actgaagaat gctcagtatt atcagccaaa agatacaggg aaatatgaac aggctctggg
    12721 tcaacagtat taccgtataa aagagtggaa aaagcatcct ccaaaaaact agacaagaaa
    12781 acggttctgt ttctgcgagc tctctcattg ctctttttaa attcatatgc tactatagag
    12841 gtgggaaatc tgtgatgttc gaattgtcct ctttgtgtgt tgaccgaaca tttttattga
    12901 tatcttggga cccgtcttgt gtcactgctg gtaacatgcc ttatcatttg gtagttcgaa
    12961 gctacgacca tgggacccac ctgcttgaat tcgcgggatc aatactacag gacaaacaac
    13021 ggcattaacg gagattacca tcttttaaag aataaagcaa tttgctcttg gcaaattgct
    13081 ttttctattt gttgcttatt ttggaggatt tgttcatgat taggatttta atgattatcg
    13141 ccactttagt attattattc gttagctatt atttatttaa taaacaggat atcttttttg
    13201 tattgatcaa caaaaatgat aaaaatcaag gattcctaca attttacggt gctgcatatg
    13261 ctgttttagg cgtaatgggt attttaacag cttttttcaa tcaacgcttt attgccttgg
    13321 tctttttact gatcgtaatt attgtttctg ccaccttcag tatccgattt gcaaaaaaaa
    13381 ttgctgaacc aaagcagtaa ataattctct ttttccctaa agtgttctac aataaaagag
    13441 tacaatacga ttgacttgag gagtgaatgc ttatgttaca acaatacaaa aaaatcatgg
    13501 ttgccgtaga tggttctgat gaagctgaat tagcttttaa aaaggcggtc aatgtagcta
    13561 tccgaaataa cggagaactt ctattagcac atgtcattga cacacgttct ttccaaaccg
    13621 tttcatcctt tgatggaatg cttgcagaac aagctactga aatggcaaag caaacattgg
    13681 cagattatga aagcaatgcg aagaaagctg gcttgaataa tgtaacctct gtcgtagaat
    13741 atggctcacc aaaacaaatt attgccagag aaatcccaga agataatcaa gtagatttga
    13801 ttatgttagg agccacagga ttgaatgctg tagaacgttt atttattggt tctgtttctg
    13861 aatatgttat tagaaacgca gcttgcgatg tgcttgttgt ccgaactgat ttagaaaacc
    13921 aattgccagc taaagaccac accaaagctt aacacgtaga cgacctgcca actatcggca
    13981 ggtttttttg cacaaaaaac cgtgataaaa acaaaagacg cggcaagcac tcatcggtct
    14041 ttataatgtt taaaataaag ggaaaacagt caatgagttc aagtaaaata aaaaaagtga
    14101 acaccagggc gattagtcgc cctggtgtgg tttaaaacga cctaagcatg ccaaagctta
    14161 ttggttcgtc aatgtggaag tttccgatga atctgatacg aaaaaatatg aagtatcgat
    14221 ggcggatacc gattttatcg ccaaactaaa attttatttc gggttcgggt accgcgagaa
    14281 ggcaagtgct acattcggta aatcattcaa gaattgatga aaggctaccg cttgccattg
    14341 caaccacaac gatactcctg aacgcgatgt tggtgagatt tccaaagaga agagctgttt
    14401 aatgcaacgg tattaagcaa atggcaaaag gaagtcatgt agacaaagtt ttttatcaaa
    14461 caccatatga tttcgcctga acgctggttt agctcagaat acaatgatgt ggtccacgag
    14521 acattaccct cagtcagata aaaaaagctc tctgacagaa ttacacatcg ataaatatta
    14581 agtaaatgtc agcgctagtt gaactttctg acactttcag gtacactgga aaagacaact
    14641 acacgaagga gggaaaatac tatgatgcgc atgactaaaa taacaagtaa tgaaaattac
    14701 caacgatacc atatcgcatt tagccatgca attggtttta cagttgctgt tgacgggaca
    14761 agtttgtcca aaacagttga attcacgtaa aatcaatggc catctcaagg tatttttctc
    14821 atgataatct gagtttaaat aacacaggcc agggataacc atggcctttt tgagtggtct
    14881 caaggagaga gatcatgatc atacaagcaa tttctattaa aaaaacaatc ggcgggaatc
    14941 cattatttga ggatttatcc tgtcaaattg aagctggtga aaaaattgcc ttagtcggta
    15001 tcaacggaac tggcaaatca acctttttac aaatcttgac cggcagagaa ggtgtcgaca
    15061 gcggtgttat cagtcgcaaa aaaggcttaa agatcgggac ggtcgaacaa gaacttacag
    15121 tttccgaagc gaccgtcaac cactacttgc tccattctgc cgctgagata caggacctca
    15181 aacagcaaat gagtcgctac gaagcattga tgacagaacc agatacaaac ttagagaagt
    15241 gtctggcccg ttacggtgag ctgcagcatc gctttgaaga actaggcggt tacgtattag
    15301 aagaccggat tgcagcgatc ttgcaaggct tagggattcc acaccgtcag gaagcacggt
    15361 taacagaact tagcggaggc gaacgagtta aagtcgcttt agccaaaatt ttagcagccg
    15421 atgccgattt acttttactc gatgaaccga ccaatcattt agatcttaac agtattcgct
    15481 ggttggaaaa ctatttacag aatacaaaaa aatcggttct ggtcgtttct catgatcggc
    15541 aatttctcga tcaagtcacc acaaaaacat gggaactgga agaaggtgca ttgattgagt
    15601 atcccggaaa ctacagccgt tttcgagtct tgaaagaagc tcgcttggct gagctgacaa
    15661 aaaattacga actgcagcaa aaagaagtcc agcggttgaa agtcatgatt cgtcgttttc
    15721 gtcagtgggc acatgaagga gacaatgagt ccttcttcaa aaaagccaaa gaactggaac
    15781 gccgcctggc gaaactgaca ctcgtcaaac cgccacctcc cccgaaaaat cggctgcaaa
    15841 gcttgtctaa tggtggaaaa tctggcaaag aagttttcat cattcaaaac ttgcaccagc
    15901 agtacgctga tcaagtacta ttcaaagaca gcagttttgc agtctatcgt ggagatcatt
    15961 tagccatcat cggcgacaat ggtgcaggaa aatcaacctt actcaaatta ttactcggtg
    16021 aggaaaagcc ttacggtggc accatcaaat taggtagtag cttgcaaatc ggttatttgc
    16081 cgcagcagct gcagtttgcc gatcccgata gccgtttgct ggcttacgcc atcacattga
    16141 ctggaaacga agaagtcgct cgacggcaat tagccaaaag tggcttttat caaagcgatg
    16201 tgggtaaacg gatcaaagat ttatccggtg gtgaaaaaat ccgtttggcg ttgctgaaac
    16261 tctttttaga aaaaatcaac gtcctgattt tggatgaacc aaccaatcac ctcgacagct
    16321 atgctcgaga agaaattgaa gaaatgctgc aagactatca aggaacgctc ctagcagtca
    16381 gccatgaccg ctactttttg cagcaacact tccaagaagc actcgttatt gatcaggagc
    16441 agatcagccg ccagccgctc ggccaactga ttgactagga cgttttttcg caacaaaaaa
    16501 cttgattttc atacctgtgc tttcacgatt caggcaaagt acaggtggaa aatcaagttt
    16561 ttattattcg atcaatgaat gtttcggatc ggttttctag ctaaacataa agcgaagaat
    16621 aaagtcgaca gcccacgagg ctgatctaaa aatgtttttt ctttttacat atgtaatgtc
    16681 ccttataaag atgaatgaaa ataaaatatt gggattttcc tgaaaatcaa taaataaaat
    16741 gccagtcttt tcccttttga ccacttttcc aagcaaaatt ccctaagaag atcctgtaaa
    16801 aaagaaggga aattgaagtt aaattaccat tttgcggtct tctccgcaaa aaaaacacat
    16861 gcaagaattc cctcttgcat gcgtttcatc ttttatccta acgaatgctt tggcttagcc
    16921 gcgcagacgc tccacatcac gggcaatcat tagttcctca tctgttggga tcactaatac
    16981 tcttactttt gattcttttg tagagatatc tacttcttca ccacgcaatt cattcaacgc
    17041 tggatcgatt tcacaaccaa accaagacat acctttgatg atttcactac gtacatgtcc
    17101 gtcattctcg ccgataccag cagtaaagac aattgcatca acaccattca tcgttgtcac
    17161 ataaccgccg atgtattttc ggatgcggtc cacgaaaatg tcataagcta aacgaatatg
    17221 ttctttttca tagttctttt caagatcacg catatcacta gacaatcctg ttaggcctag
    17281 cagaccagat ttcttgttca aaacattgat catttcatca atatcaatct ctaatttttc
    17341 cattaaatat gggagaacag ctggatcgat atcacctgaa cgtgtaccca ttgtcacccc
    17401 agctaatggt gtgaatccca tagaagtatc catggattta cctccatcga ctgctgtgat
    17461 cgatacacca tttcctaagt ggcaagtgat gatttttagt tcttcgatcg gtttgttcaa
    17521 taattttgcc gcacgttccg acacataacg atgacttgtg ccatggaagc catattttct
    17581 tactttgaaa tcttcataat aagacaccgg taagctatat aaataatttt gctttggcat
    17641 tgttgagtgg aaagatgtat cgaatacagc cacactcaaa atgtcaggta aaatcttttt
    17701 aaacgctttg atccccatta agtttgcagg attatgcaaa ggagcaaatt ctgctaattc
    17761 agcaatgcgg tccattactt tctcatcgat cactactgaa tccccataag tttctcctcc
    17821 gtgaacaaca cggtgcccta ctccggtgat ttcatcataa gagcttaaaa tttgcagttc
    17881 aatcaactga tctaacagca tttttactgc tacttcgtgg ttttcgatat ccatgatttg
    17941 ttcgaatttt tgattatcgc cgtacttgat ggtaaaaata gaatcttgta aaccgatacg
    18001 ttcgacgatc cctttcgcaa ctacggtttc ttctggcatt tgatataatt gccatttcaa
    18061 acttgaactc ccagcattga ttgcaattgt tttagacata tgactctcct tctcgttgaa
    18121 tgattacaga gcagatgatt tccagttttt aaactcgttg aaaaattccg tcaccttctc
    18181 tggttctttt aatgaagcaa gtttcacaag caagacttct tttacttgtt gtgcttgtgg
    18241 tccttttttc tgtaagatca aaatactttt ttgcgattgt ttattacgga acaattcatc
    18301 tggcaattga atcattcctt gaagataacc ttcttcttta aaccattttt tgatttcttc
    18361 actttgatcc gtttctaaaa atccactcgg catcaaaaac aaaccaaaac catccggctt
    18421 cacatatttc attgattgct ctagcaataa atgatgtgca taactatggc cttcttcggt
    18481 acttgttaaa aactcttttg ctttttcgtc attcggataa taaccgattg gcaaatcgct
    18541 gattgcaaaa tcgacaggat ctattaataa ttcctgcaac ccgtcttgat gaaagtattg
    18601 aacatttgct tgtgtcaaat cacttgtaga agctgcgaca gccaacaatg tatcatcaat
    18661 atccacacca attccttctg tttggtaccc cgcattcgat aggtttaaaa gaactgttaa
    18721 taacaagttg cccattcctg ccgcaatatc cagaatctta accggtgctt ttttatttgt
    18781 gtatagttgt tcaataagaa aaacaaataa aaaaccgata ctatcaggag tgagttgatg
    18841 gttcgcctgc aaatgttctg tctggctgcc cttcaataaa agtaattgag acagtctgcg
    18901 ccattcttct ggctcgaaag aaagtttctt caactcttca taaagagctg tgatccgttg
    18961 cgttgtctct tttgtcggca ctccatccac cacacgtact tgataatcat ctattaaatt
    19021 ttctgcattt tcgacatagg cttctaaaaa ggaagttccc aatgcattct gtaaaagctg
    19081 aatagcttcc aggttttgat taaacccttg ttccattttt tctggtaaca tggtgcacct
    19141 catttctaga tatattataa caattttttc acctgtttaa ttctatcgaa caattgacaa
    19201 aatttcaatc gttttcactg ttttttattt gtgaaacggc aaacattttt ttattaaaaa
    19261 cagtgttata acagaaaaga gggcgttttc acgaacattc catcattctg tactagtatc
    19321 tgtatcattt tttacttctt ctgtgaaccg aaagataaac ggtgagactt ccacctctaa
    19381 gaatagggac tgttgtaccc actgatatgt tacagatcca gttgtgtagt aaaaaactcc
    19441 ttccgaactt tcggcaagcg tagactgttc cgataaagcc atctgcttca ttatttttgc
    19501 tatataataa tttttgattc gtatattaag atccattgac aaacggtagc tttctaatgc
    19561 tgccatcaat aaaaacgtac ataaaagaaa taaaagcaag acagaaaata aaatagagcc
    19621 cttataacat tttctactga tcgttttttg tccatatggc atatccatct tctccatttt
    19681 caaagtgtac ataaaaagaa attgtgtgtt ctttttccaa taattgaagc tcagtgatac
    19741 ttgttaacat aggttgatgt ccgccggaag ttcttctttt acgtatcatt gcctgatatc
    19801 tttcgatgat atactgttta tcctcgatcg tgtagtgcag ttgctgattt ttcacagaga
    19861 cattgtgact gctcttgagt tcttcttcta attgtgctaa aaatattagc cactcttttt
    19921 gatctttacg ctgcaaaaat tcacttactt ggatggtttg ctgaatcaaa agattgaatg
    19981 tgagcaatag actgctcaat atcatcagtc caattagaca ttcaatcagc gtaaatgctg
    20041 gaattacttg ttttttcaat actgatcgct tctttctcca tattgacaaa cactttttcg
    20101 attactccat tattatcgac aatttggata tcgtatggga tcatttcttc ccttcgctct
    20161 ggtcttttgt gatacatccg ctgcgtcttg atatcttcat aaatcattcg aataaatagc
    20221 agttcctccc cacttttctt ttctgcctga agcaaaaaca gctggccaaa gctcactaag
    20281 tagacacaga cagaagcgat cataagcgct gtaagggact ccaacaaaac aaatcctgtt
    20341 ttattcaatt ctttttgtat agcgaccact ccctatctgg aattgatacg taatcgtccg
    20401 ctctttttct tcccaataaa attgataagc ttttagtgaa ctttcattcc cggttctagc
    20461 agcaaaagta atagaagcat ggcgtttcaa ggtaatctca tcaggaatat ctaatacttc
    20521 ccaatttatt ttatctggat tcggaacatg aaaaatgatt tgattctctt cactattcca
    20581 ataaaagcct gtttggatct gattcacaat agcgattttt tgcgtagaat agattcgttt
    20641 ttcaaattgg ccgaaaaatt gatagactgc caattcattc ttccatgcag aaaatgcgag
    20701 aactggaaaa gaaaggatcg ttgtaagtaa taaaatcagt aagagtgatt caaacaacgt
    20761 atatcctgat gctaaaacct taccttcaag gccgctgata gatttctgca tgttctcgtt
    20821 tgatatagcc ttcattcaac aattcctcca cagtaggaac tcttccatta ttttgaagcg
    20881 catagagctc tttttgtgtg tctaccaact ggatgatcgc ttcacgactt tcttggtcta
    20941 catgattcct ataacgcgac aaattaggga cgaacaacaa cactaataca ctgatgatca
    21001 gtaagacgat tagcatttcg atcaatgtaa aaccgctata ataagatatt tttctctttt
    21061 tcatggcaaa acctcctcta tgcttccgta aacaggtaat aaaacagcgg cataaactaa
    21121 taaaatcaaa gccgctacgc ccaaaaaaat gattggctga ataaaatgca tccagtgatg
    21181 aagacgtttg aaaagctgtt ttcttgtcca ttcgctgtaa aaaatcagtt ctttacctaa
    21241 acttcccttt gcctctccct gtaaaacaat tttactgaat tcttctgata gaaaacgata
    21301 attctggaac tgctcagcca atgacatacc tgcctccaat ccttgaatca acttagaggc
    21361 aagatgctga atcagacttt cttgtttcgt ttcctttaag cagactatga tttgccttaa
    21421 ttcgaaacct tcatgaaaaa gctttccaat ctctagtgac aaataagccg attgatacaa
    21481 ggaatatatc ggtccaatca ataattgtct gcttatccat tcactctgat caacaggaga
    21541 caacttcatc agacgatacc ttactagtaa agccaataga gtaaccgcta ggatgaatag
    21601 tagaacatac caatgaaaga cttgtatcag ctgaataccc caatgctctt gcatgaccat
    21661 tcctgaacca ataagctgag gcaaaacgaa caaccgcata ctaaacaaga tacctagcaa
    21721 aaaggtgagc aatagcatcg gatatgcaag cagctttttc aaatcattgc gaaatccttc
    21781 aacaagccga aactgtttgg caatttccaa caaagttgtc ggcagattcc catgacgttc
    21841 agctaactcg atctgtacca gttgctctgc tccatatcct atttgcgaaa gtacctcact
    21901 aaacgcttgc ccttccttta gctgatgttg gatatttgcc aaaataacag gtggaaacga
    21961 acggatcgtg agtaataaat aaagggattc ttgcagacta aaaccagata aaagcagttc
    22021 tcccatggtt tgtgcaaact ggttttgctg gcttcttgtt aatcctttag aatagttctg
    22081 actgccaaat attttcctga gcttcttcat aactttcctc ccatgatttt tttactgatt
    22141 cttcctccat aaattgataa ctccataaaa cagaaggatg gtgattggca tcttccaaaa
    22201 gttcttgata aatgacacca gctaaacatt cagatagctc ttcttttttc tttgtcagtt
    22261 catggattct gaccaatgta ccttcaagat tgcgtgcatg tacggttgca aaaacccggt
    22321 gccctgttag tgctgctctt accgcagcat gtgcagttga ttcatctctt atctcgccaa
    22381 tgattagcaa gtctggacga tgccgtaatg cgagtttcaa aagtgtatca taagtctgtg
    22441 ctattttcgg attgatctgt agctgcagaa aaaacggttc ttctatctct acaggatctt
    22501 cgatagtaat cacttgcaat ttttcttcca aagccaaccg atacattaga gaagtcttgc
    22561 ctgatccgac tggtccgctg aaaaggtata atccacgtct gacggttttc tgtaaaatag
    22621 ttgcaaaatc tcgagaacta aagcaacgat ggggtgtttc aaaagtaccg tataatagcc
    22681 gaatcactaa actctctcgt tgaaggtaat ctcctacaga agacaaccgc agacgaactt
    22741 cctgtttttc taaagaataa gtaaatgcac ctaactgagc ttttcttttt tctccgatat
    22801 ccatctgtcc caaatactta aatcttgaga tcagttgctg tccatctgcc gaagagactt
    22861 tcttccagaa tgttcgtttt tttccatagc gaaaatagat gtaaaatact tctgaagctg
    22921 gcaatatata tacgtcttgg gcatgtttgg caaaactttc acttatcatt tgatccgata
    22981 atcgttttat ttccattatt ctcccctcca caaaaaacat acgtcttttt gatctttttg
    23041 tttcaaataa aatgaattta caaaaaaata ttggcaaaag ccgaatttat gttatactta
    23101 taaaaattta tcttgggagg cactccattg gaaacaacat taaggaaata tatcgaagta
    23161 gcaagtaaaa aaaagcctgc tgacctagtg atcaagcatg ctaagatcgt caacgtcttt
    23221 acgaaagaaa tcatggaaaa agatgtagca atctgcgaag gaatgatcgt aggaatcggt
    23281 gaatacgaag gaaaaagcgt acataatgct aatgggcaat atctagtgcc aggattcatc
    23341 gatggccatg tgcatatcga aagctcactt ttatcaccaa gcgaattttc aaaagtatcc
    23401 ttgcttcatg gagttactac tgtagtgaca gatccccatg aaataggaaa cattgctggg
    23461 agtacaggtc ttgagtttat gatagaagat gcaagacaaa cactcatgaa tatctttttt
    23521 atgctacctt cttgtgtacc tgtaacccct tttgagacca atggtgcaaa tttaaatgca
    23581 aaaaaattag cccctttttt acaaaaaccc gaagttttag gcttagctga agtcatgaac
    23641 tatcaagcag tagcaaataa tgaaacagac atcctagaaa agatccaatt gatgcaccag
    23701 cataagaaaa aaattgacgg gcatgcagct ggtatcggca tggaagagtt aaatgtttat
    23761 cctgcagcag gtattcggac ggatcatgaa gctacaactg caaaagaagc taaagaacgt
    23821 ttggatcttg gtatgtattt aatggttcga gaagggaccg ttgcaaaaga tctggcatcg
    23881 ctactaccgg cgatcacacc tgaaaattca agaagatgct tctttgtcac cgatgataaa
    23941 ctgatcaatg atttagtaaa agaaggatca atcgatcata tcgtccgtca agctattgca
    24001 cttggaatcg atccattaca agccattcag atggcaacat tgaatgcagc agaatgtttt
    24061 gatttgaaaa cactaggtgc gattgcccct gggtatcaag ctgatttctt cttaacagac
    24121 gatcttacca agctgccaat tcatgaagtt ttctataagg gaacattagt tgttcaatct
    24181 gggcaactcc aagaccattt atttgctgat cattcaaatt catatacgtg ttgtctgcca
    24241 aaactaaacg ctcaaccatt aaatccacat tcgttagagc tgcttctttc ttcttcacaa
    24301 gcccatgtga tcgaaattat cccgaatagt ctagtgacta aagatttggt agaaaaagtg
    24361 gaccgacaag gcgaacgatt cattccttca atcgaaaaag accaattaaa gattgcggtg
    24421 atcgagcgtc accatcaaac gggaaacatt ggattaggca tagtgaaagg atttcagctg
    24481 aaacaaggag cgatagcgac aacgattgcc catgactcgc ataatctagt tgtcgttggg
    24541 acaaacgacg aggatatgct gtatgctgct aatcttttga ttcaaaaagg tggcgggatg
    24601 accgttgtca acaaacagca ggaaatagct tgtgtttctc ttcctatagg cggaatcatg
    24661 acacaggaac ctttttcgat cgtctatgaa cagttgaatg aattaataac taaagcacat
    24721 caactaggag cagctaaaac ctttgatcct tttttgacac tttctttctt aacattatct
    24781 gtcatacccg aattgaagat caccgacaaa ggattatttt cattttctcg cttccaattg
    24841 attccacctt gtggaaaaac cgatgaaata aatacatttt aaatcaaata aatcccagaa
    24901 aatacattac atctgtaatg ttcattttct gagatttatt tatggatatt aatgttttta
    24961 tgaaagcaga aagttagttt cacagacttt tctttttggt cttatcttga aattgagctt
    25021 ctactcgata aatacgttga ccttttgaag aaaatttttc ttcatactcc gtcataatat
    25081 tgccttcaaa ttgactttga tgtaaatcta accatacctg tttgaggatc atgccatatt
    25141 gagaaaaact tgccagagaa tactcaaaca atccttgatt atctgttttg aaatggattt
    25201 ctccatttgg acgaagaatc tgttcatcta cagcaagaaa agtcttatat gtcaatcgcc
    25261 gtttttcatg acgttttttt ggccatggat cggaaaaatt cagatagatt tgatctactt
    25321 cgctgtcagc gaaatactct gtcaacgctg atccatccac atgtagaagt ttcaggttag
    25381 gaagcggttg ttcgatcaac ttgtctagtg caatcgaaac aacactgacc tgcatttcta
    25441 ctccaatata attgatttca ggatgggctt ttgccattcc agtgatgaac tggcctttcc
    25501 ccataccaat ctcaatgtgg attggatgat cgttttcaaa caattcattc cattttccct
    25561 tccacaatgt tggatcacta attacaaaat tcgggtgagc cgctaacatt tccgctgctc
    25621 ccggacgatt tcggacacgc attatttatc tccattcctt tcctctaatt aaaaaggtct
    25681 ggccaaatct ccgcagagtt tgggccaaac ctcagccact gaaaattcgt tttaattgta
    25741 aaatttcttc gttcatccgt cgatgatctt cacgtttgca atatctggtg atttcttgga
    25801 gcaaattcat ggccgcatac caatgaaatt tttcaagcat ttcgtcagtc gggcgtatgc
    25861 cataactgat cagccactga ttccagctag acagtggcac ataatttcct aaaatcgttg
    25921 caatatccac cgcagggtct gcaaacatga ttgaatccca atcgactaga tataaataat
    25981 ttttacatac taaccaatta cgatggttta catctccgtg tactactgat aagttttcct
    26041 tatctattga cgggatctgc tcacgaagaa aacgatggac aatttgcaga aattgatttt
    26101 tctggagttc ttccggtaat tctttttcat atttttgcag catcttctcc ggcgtcatga
    26161 cacgaccacc gatacgggtc agcatggttt taagagaatt tgaatgatgt aaatggtaca
    26221 aaacatcaat cacgtcgttt ctttgaccaa tctcattagg agtcagcaag cgaccttcca
    26281 gccactcttg tgctgtcaaa gtatcgccat tccccgttcg ttttgtccaa acaagttttg
    26341 gtgtaattcc ttcccttgaa agagcagcca acatcggagt cgtgtttcgt ttaataaaga
    26401 ctttgtcctc atcacgcatg cctatgtatg tttttcctgt gtcgcctttg atgggacgta
    26461 accgccactc ttggtctaac tgaaactcca tcatgcattc tcccgtcctc ttactctttg
    26521 ttttgcagta cttattattc tagtcgcctg aacactacac gtcaagcaca tatacgttct
    26581 ttttcattta attgccattc aaaaaaccag cttttctcaa aaaaaataga acgaacagca
    26641 ttcttactgt caacgctcta tttttcattc tcatttttac cctacacagc tttgttttca
    26701 gtctgacatc ttcttcaaac gagttggtac atatagttta acaaagagca tagtaacaat
    26761 caaataagcc gctagcggaa cccatcgctg ctgcccatct aatacgactg cagcaatcaa
    26821 accaaaaata accgacgcga taattaatac ccatgtgagt agatactgaa tcgcctgctt
    26881 cttctgattc ttatggattg gataaagctg aacgagaacc atatagcgga actggttcgc
    26941 taaaggtaac agttggaaac cgatcaaata taaaaataag aaaccgataa tcagactaaa
    27001 ataaaagtcg actactccgg caatcaataa cccacctata attactaatc ggaaataaag
    27061 accactaaaa tcagaacctc ttgccaatct tcttgcaaac aagtaaagat aagtgttttt
    27121 tgattctctt ttaatatgag tcaatatagg atccaaatag cgtcttcttt tgatctttgc
    27181 cgtaatttca ggaacatctg tgaataaatt gataaattga tagatctgat gcaacctatg
    27241 ttcttctaca tgcaccattt tctcccaatc caatcctttt tcttgttgct tccacaaaaa
    27301 ccagtaatat agacttgcct gtactaatgc acctgctatt cctgcccata ttagccaata
    27361 aaatgataga aacaaaaaga tcagagaaga aaaaaaccag cctaatttcc aataacgaat
    27421 aacttgttga tctagtcgaa acaattctgc tctcttgatt tgcagatgac tgaatttcaa
    27481 actccataat atgatcagat agacgaaaaa catcgaaaag gattgctccg tactaaccac
    27541 cactaatggc atggccaatc cgcatgccaa tatcaaaaag atcaacggaa aaatgcaaga
    27601 atagcgaaaa gctcgttcca aataggtttg catttctttt tctttaggaa gtaaaaaaac
    27661 aatatctgct gcctctgcaa aagtagccat cttacccacg aataaacaga gcatccaaaa
    27721 tacagagatt atgagccctc caattgcaaa cggtggctgc agggttttca accaattaga
    27781 ataatataat cccacaccgc caatcaagaa aagacaaact aaaacaaaat gatcgttcaa
    27841 tacataacgc atatatttca tcattttctt ttgatgtctt gctaaacgtt tttgaaaaaa
    27901 actactcatg tgcctgctcc tttgttaaag caatatagat ttcgtccaaa gaagaatccg
    27961 gcagctgaaa ttcttttctc aacatctcaa gagaaccgtc tgctcgtatc tctccgttat
    28021 gtaagaccac aaaacgatca caatattttt ctgcagtagc taaaatatgc gtagacatca
    28081 aaataccggc tccttctttt ttcatttcta ccatcaattc aagcaatgca ttgatagcta
    28141 gtggatccaa gcctaaaaat ggttcgtcga tgatatataa agaaggctcg atcaaaaagg
    28201 cgcataagat catcactttc tgtttcattc cctttgaaaa attagctggg aaccagtcaa
    28261 gacgattaga taaccgaaaa gtctccaaca acttttctgc tcttttcatt gctatatcca
    28321 taggtatatc gtaggccatc gcagtgactt cgatatgttc tctcaatgtc aattcttcat
    28381 ataaagaagg agtctctgga atatacccaa tttgtttgcg atattcttcc gtatttttct
    28441 ttaaggatag atgattgatt tctatcgttc cttttgaagc ttccagcaga ccgataatgt
    28501 gcttgatggt cgtacttttc cctgctccgt tcagtccaat caaccctact atctctccag
    28561 gctgtacatc gaaactgata tcttttaaca cagggagatg tccgtacccc cctgtgacat
    28621 gatcgataat taaactcatt ctttttcctc cgccatttat ttttatttat tataccacga
    28681 agaaaatgac ggtattttgt ttttttcctt acaatagctt taacttatgg taaagttaag
    28741 ctgaagtatt tattctttat agaaaggatg ttttatatgg aaaactgtat tttttgcaaa
    28801 atcatcaatc aagaaatccc aagctataaa atatatgaag acgacaaggt ctatgctttt
    28861 ttagacatct ctcaagcaac gaaaggacac acattagttg tgcctaaacg tcatgtagca
    28921 gatatttttg aatatgatcc cgaacttgca ggagaggttt tttcgcgtgt gcctaagatt
    28981 gctcaagcat tggaaaaagc ttttccagaa atgaaaggtc ttaatatctt gaataacaac
    29041 cgtgaactag cctatcaatc tgtttttcat tcacatatcc atttagtccc tcgctacagc
    29101 aaagaggatg atttttctat tcactttgtc aatcatcaag atagttatgg atcggaagaa
    29161 ctaaaagcta ttcaagaaac aatcgtaaag caggtgtcct gcgatgatta aaaacttttt
    29221 caaaggactg ttatttggag ctgctgctgg cactgtcgga ggtctgttat tggcgcccag
    29281 gagcgggaat gaaacaagaa agaaactgat cgaagaattg gaagaagcaa caactctgac
    29341 aaatgattta aataacagcc tgaatcattt taaaagcgca ttgatcgaaa caaaagaaac
    29401 agctgaatct gtgattcctg ctttccaaga agcaatcaaa aaagatatcg aagattttaa
    29461 attccaagcc gaaccacgta tcacacaaat caatgaacaa gtagaaaaac taaaagctga
    29521 tctgccagag tatcctgaac aaatataata atgattattt tctcaacaga tttaactata
    29581 taagtgagaa aagtttgcgt atttcctagg aatgtgatga aattttgaat tttgtttgaa
    29641 actaccacga caacgttttt aaaggaaatg ggatatggta tagttaatat ataaaaataa
    29701 taactaatta caggagtgca aattttaatg aaaaaaaaat caattatttt ggcagctact
    29761 agtgcgttgg ctgttttaac tctagcagct tgttcaggcg atactaataa agatatcgca
    29821 acgatgaaag gcggaacgat cactgtatct gatttctatg atgaagcaaa acttgaatca
    29881 tcgaaccaat ccttagtaca acggatgatc atttataaag tattcaataa caaatatggc
    29941 gataaagtaa ccgataagca agtggatgca gaatacgaca aacaagcgaa atcattaggt
    30001 gatacttttg aatcacaatt agaagctgct ggatatacga aagatactta taaagaatat
    30061 attcgtaaca atttagcttt tgaagctggc ttgaaagctc acgtagatat tacagatgac
    30121 gatttgaaaa ctgcttggaa atctttccat ccagaattag aagcacaaat catcaaatta
    30181 tcaagtgaag atgaagcaaa agatgtgaaa aaatcagcgg atgatggaga tgatttctct
    30241 aaattagcca aagacaaatc aactgataca gagacaaaag aagatggcgg taaagttaaa
    30301 ttcgactcaa cgacaacaac tattcctgca gaagtaaaag aagcagcttt caaattgaaa
    30361 gacggtgaga tctccgatgt tattactaca acaaatccta cttcttatgc gacagaatac
    30421 tacgttgtca aaatggtgaa aaaccaaaat aaaggcaatg acatggataa atacaaagat
    30481 caactaaaag atatcgctac tgaaactaaa ctaagtgaca atgcattcac aactaaagta
    30541 atcggtgaag aattaaaaga tgcaaatgta aaaatcaaag atgatgcctt tgaaaatgtg
    30601 ttgagtgcat tcacaacaac ttcatcaagt acgaaagact caagtgaaac tacagcttca
    30661 acaaagagtt ctgacacaaa atcaactgat tcaacaaaag aatcttcaac tgaagaaaca
    30721 actgattcat caaaataaaa gaaaacaaaa
//
LOCUS       DACEPW010000013        28867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEPW010000013 DACEPW010000000
VERSION     DACEPW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.013"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(402..1355)
                     /locus_tag="IW819_000465"
     CDS             complement(402..1355)
                     /locus_tag="IW819_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1243595.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1378..2133)
                     /locus_tag="IW819_000466"
     CDS             complement(1378..2133)
                     /locus_tag="IW819_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1243596.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2130..3089)
                     /locus_tag="IW819_000467"
     CDS             complement(2130..3089)
                     /locus_tag="IW819_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1243597.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3082..4032)
                     /locus_tag="IW819_000468"
     CDS             complement(3082..4032)
                     /locus_tag="IW819_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1243598.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4182..4634
                     /locus_tag="IW819_000469"
     CDS             4182..4634
                     /locus_tag="IW819_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1243599.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4723..6990)
                     /gene="gshAB"
                     /locus_tag="IW819_000470"
     CDS             complement(4723..6990)
                     /gene="gshAB"
                     /locus_tag="IW819_000470"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1243600.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7177..8175)
                     /gene="ftsY"
                     /locus_tag="IW819_000471"
     CDS             complement(7177..8175)
                     /gene="ftsY"
                     /locus_tag="IW819_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1243601.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8186..8995)
                     /locus_tag="IW819_000472"
     CDS             complement(8186..8995)
                     /locus_tag="IW819_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1243602.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9004..12585)
                     /gene="smc"
                     /locus_tag="IW819_000473"
     CDS             complement(9004..12585)
                     /gene="smc"
                     /locus_tag="IW819_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1243603.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12605..13291)
                     /locus_tag="IW819_000474"
     CDS             complement(12605..13291)
                     /locus_tag="IW819_000474"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1243604.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13639..15420)
                     /locus_tag="IW819_000475"
     CDS             complement(13639..15420)
                     /locus_tag="IW819_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1243605.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15446..16360)
                     /locus_tag="IW819_000476"
     CDS             complement(15446..16360)
                     /locus_tag="IW819_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1243606.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16387..17349)
                     /locus_tag="IW819_000477"
     CDS             complement(16387..17349)
                     /locus_tag="IW819_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1243607.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17349..18293)
                     /locus_tag="IW819_000478"
     CDS             complement(17349..18293)
                     /locus_tag="IW819_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1243608.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18294..19298)
                     /locus_tag="IW819_000479"
     CDS             complement(18294..19298)
                     /locus_tag="IW819_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1243609.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(19566..19808)
                     /gene="acpP"
                     /locus_tag="IW819_000480"
     CDS             complement(19566..19808)
                     /gene="acpP"
                     /locus_tag="IW819_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1243610.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(19930..20931)
                     /gene="plsX"
                     /locus_tag="IW819_000481"
     CDS             complement(19930..20931)
                     /gene="plsX"
                     /locus_tag="IW819_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1243611.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(20980..23016)
                     /gene="recG"
                     /locus_tag="IW819_000482"
     CDS             complement(20980..23016)
                     /gene="recG"
                     /locus_tag="IW819_000482"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1243612.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23133..24815)
                     /locus_tag="IW819_000483"
     CDS             complement(23133..24815)
                     /locus_tag="IW819_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1243613.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(24867..25229)
                     /locus_tag="IW819_000484"
     CDS             complement(24867..25229)
                     /locus_tag="IW819_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1243614.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            25499..25687
                     /locus_tag="IW819_000485"
     CDS             25499..25687
                     /locus_tag="IW819_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1243615.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            25925..28594
                     /locus_tag="IW819_000486"
     CDS             25925..28594
                     /locus_tag="IW819_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1243616.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         7943 a         6223 c         5148 g         9553 t
ORIGIN      
        1 gcttctcata ttttctagtc tagtggactt attgccgaga agcagcagat ggaacatcgt
       61 ttaaagaaga gatcggtaca aaataaaaat atctaaattt aagttttatc taagtttcgc
      121 tttgtacctt tgattcaaat caaaggaggc gatcttgatg ctgaaaaaat ggatcacact
      181 attttttgcc actccccatt ctagaatcaa cccgtatgat gtagataatg aattcacgtt
      241 gaaaaaagat ttctcaaata aataaaaacc atccgtaaaa tcatcaatac gattttacgg
      301 atggtttttg ataacttctt tgaggatagg ctcttgctat ttaagaatgt aagaaacatt
      361 attgttacat agtatgagag tttaatcctc tgttatttat gctatttaaa tgcttgattc
      421 acatcttcga tcatcagttt cattgattct aaaccgcccc cgcttaaata ccagacatct
      481 ggttcaaggg agatgatctg ctgattttta ccagcattcg tttgtgctac cagttcattg
      541 gcagaaatat catctttgct gtcgtctcca ccaatagctt ttgttctgtc tactacaaat
      601 aagatatccg gatttttttc cagcacatat tcataagaaa cactttgacc gtgagtggat
      661 gcttcgattt gatcatctgc ttgttcaaag ccaaataaat catggatgaa accgaatcta
      721 gaaccagatc cgtaggctga taattgccct tcattcacaa gtgtgactag tgcttttttg
      781 tcagtagctt ctgctttctt tttcgtctga tcgattgcac tagataattc ctctaattga
      841 gttttagctt cttcttgctt accaaagatc tcccctaaag cagtgacatt ttgctggaaa
      901 gattcccatg gatttttcgc atctaaagaa aggaaaatgg tcggagcaat ttgacttaga
      961 tctttttgaa aatctctttg tcggccagaa atgatgatta gatctggctg aagctgatta
     1021 attttttcta agtctggttc ttttattcca cctgctgatt ctactttttt ataggatgaa
     1081 agatactcag gaaggctgct tgtagctgct ccaactacct tatctccaac accaagagca
     1141 tccatcgtat ctaaagaacc attgtcgaat acgacgactt tttctggatt atttggcacc
     1201 tcgatttggt ctccattact atctgttatc gtcaatgtag tttcagtttg tgtgctgctt
     1261 ttttttgtgt ctgcctgact ggtgttgttg ctatttccac atgctgctaa accaaatgat
     1321 gctagaatca agatacctac tactaatttt ttcatctctt ctctcctaat gttagtttta
     1381 gaaataaaga cagaatctgc ggccttcaat ctcacaaatc cgtatgtcca tttcgtataa
     1441 ttcatccaat acatcttttt ttatcatttc ttgggtcgtt ccttgcgtaa ataactgtcc
     1501 atctttcatt gcaacaattt cgtctgcata acttgcagca aagttgatat catgaagaac
     1561 aataacaatt gttttcccga tttcttgtac taaatttttt aaaatctgca tgatttgaac
     1621 cgcaaaattc atatctagat tgttcaaagg ttcatccagt aaaatataat ccgtgtcttg
     1681 cgctagcacc attgcaatat acgcacgttg gagttgaccg cctgaaagag tgtcaatcga
     1741 ttgatccgct atttcttcta acccaaggtg ttccagtgat tcctgaacga tttcctcgtc
     1801 ttttgtcgtc aattttcctt tcgtataagg aaatcgtcca aattctacta attctttcac
     1861 ggtgatttgg acatttattt cattcgattg tcgtaggatc gataattttt tggctaattc
     1921 attggatttc caagccttta cttcgttatg gtctaaatag atttcacctg tatcttttgg
     1981 tatcaaacga ctgatcatcg acaacaccgt tgactttcca gcaccatttg gaccgataaa
     2041 agcagtcaac ttcttttcag gaatcgttaa ctgtacatct ttaaccactg attttccatg
     2101 gtacagttta ttgacctttt gtaaatcaat cataggatac tcttcctttc gcgaatcaat
     2161 tttgtgacaa aataaattcc tccgacaaat tcaacgacga cactcactgt tgtattccag
     2221 ccgaaaatct gctctactgc gagctgtcct cctgtcagaa acagcatagc taccagaaag
     2281 cttccgataa atagctgacc atgacgataa gtagaaaaga cacgataact gatatttgcg
     2341 acaataaacc ctaaaaaagt aactggaccc acaagagccg ttgatgtccc tgtcaaaaga
     2401 ctgacacaac ataaaatcaa aaattcaaaa cgatctgtgt ctatcccgag gtttactgct
     2461 tgttcatttc cgagatggaa aacgtctagc acatgtcgat atcgaaataa caggtaccag
     2521 cagccgataa tgatcaaaga agcaggcaaa agatactgac tctggatatt gccaaagctt
     2581 gcaaataatc tgccttgaag caaatcatat tcattcggat ccatgatgac ttgtaaaaag
     2641 gtactgacac taccaaaaag cgtacctaaa atcatccctg acatcagtaa taaaaaaaga
     2701 ttcgctctca tttttttcaa aagaaagaaa aataacaata gactcagccc gaccatcagc
     2761 ccaacgttca ataagaaaaa aactgtacct gtctgctgaa gcatcgtcac tcctccgata
     2821 aagaagaaaa ggaccgtttg gacaaggaca tataacgact caagtcccaa tactgaagga
     2881 gtcaaaaact ggttatgcgt aattgtctga aagctgattg tggcactgct gacagctagc
     2941 gagacaagga taaatgcaaa aattttctta ctcctcaatg cacatgcaaa atgccagttt
     3001 ccatatgtat ggatcgtcaa gtacattcct gtaacaaata aaacaagaac aagcatacca
     3061 ataaaaatag atggcttttt tttaagcata tcgtcgtccc cttatcagaa gataaaggaa
     3121 gatagcccca ccgatgattc caacgatcac actgactggt atttcataag gaaaaatcaa
     3181 cacgcgagcg acaagatcgc aaaatactaa gaacaaacta cctgctaaag cagttacgcc
     3241 aattgttttg tgcacttgat ctccatatat tctagagaca agattcggaa tgatcactcc
     3301 gataaaagga atgcttccta ccatgatcaa catgactgca ctagccaatg atactaagcc
     3361 aatgcctaaa aactgcatct tctggtaatc tattcccaga tttactgcaa acgattcgcc
     3421 cagaccaatg atcgtaaatc ggtaagcaaa tagccaaacc aagagcaaca atggaacaac
     3481 agcatacaat aactcataat ttcctcgtat cacagtcgca aaattccctt gaagccatga
     3541 agtcatattt tgtaccaact ggaattgata agcaaaaaaa gtagcaactg aaccaatgat
     3601 attccctaac atgactccaa tcaacggaag catcaattga tttttctgga ggaacaatcg
     3661 cgtaagggat aaaaaaagca atgttcctgc aaaagcaaag caaaatgctg tcaaggatcg
     3721 gacaatcaag gatgcaccag gtaggaaaat catagccact aaaataccta aacgcgcact
     3781 atccatcgtg cccgcagtcg ttggtgaaac aaatttattt tgggtcaaat gttgcatgat
     3841 caacccacaa atactactgg tcgctcccac aaggatcaaa caaatcgttc gtggcaaacg
     3901 agtcactagc aagatttgta agctttgccc atctaactgg agcagttctg acaattgtac
     3961 attcgatact ccgatgaaca atgaaatact acttaaaata actaataatg ctactagtcg
     4021 tccgtagcgc atccaatcca cctttctaat tgagaatgat tttcattctt attttaagtg
     4081 aatcgttgaa caaaagcaac cgctttttca tatgttttag gtattattgg gaatatgcta
     4141 taatcgtaag aaagacaaag agaagaagga gggtattttt tatggcacaa gatgatgtaa
     4201 aaagccattt ggataaggga ctttatggta cacctttagt gaaccctgaa gaacagcata
     4261 aatatttagg gacattcagg gaacgctgct atcttagtat gactgttgct gaaatgatcc
     4321 aaccaaaaaa caaagaaaac tttttaaaag aactcaaaaa acatccagat gcttccatac
     4381 tcttgaatgg ggctctaccc ctttctgtcc aaaaccaata tatccagcta gcaacgaaaa
     4441 acaatagccg tttcactgta gtcaacgatt acgtcactgc aaatcctgag gaattcggat
     4501 tgctcttaac agcaaaagaa gcagtaaatg aacctgtgat tgatatcgaa aaaaaatacc
     4561 cagaagaaaa ctcttcgcca gctgaaaaag aaaaaccaaa aaagaaaaaa agcttctggg
     4621 atcgtttatt ttaaaacgaa aaaacacata aatttttaca tgcaaaaaaa gcttttgatc
     4681 agattgacat caaataggac aatctgtcaa aagctttttc ttttaaaata cttcaggata
     4741 taacaatttc aatacattca tcgtcaatct tctgcctttt cctgcaaaag gataaacatg
     4801 catatgcatg gctggattga aatttgcttc gatgatccca tatgcttttt tatcagttgt
     4861 aggatcgatt tctttatctg gtataatcaa atcgatacca ctaatcttag ctcctagtgc
     4921 ttctaccgca gaaacggcga tttttttgta agcttcagaa aattcatcag tcatatcgat
     4981 cgaatcacca cctgtactga tatttgagtt ctcccttaga taaacaattt gattggcttg
     5041 gggaactgat tcgatcgtca acccttgttc tttcaacatc aattgttcta attcacctaa
     5101 ttggatcaat tctaaaggtg cacgatgatt cgttccacgc aaagggtcac tatttttttc
     5161 ttcaacaagt tctttcactg ttcggatact gtcaccaata acattagcag ggactcgcaa
     5221 catgatggct tggacttctc cgtcgatcac aaagaaacga tattccgttc ccggcataaa
     5281 ttcttctact aaaaccgaac tgtcttcacg aaaagcaatg gctaaacctg cttgataatc
     5341 ttccagacta gctccttcct tgaaaatcgt gatacctaac ccgtaattgg ttgatttagg
     5401 tttgatcaca aatgcctgct cggcaaatct cggataagct tttaccgctt cttccatgga
     5461 tgaaaattct gcgcctcctg gtacccggaa cccagcttct tttaagactt ttttcgttac
     5521 tgttttgttt tccatgataa gtgggacgat atagctgtcc ttacttgtca tgtttgcatt
     5581 tttcacatat tctacatggt cttggaattg cagacgcaaa aattgatccg actcatctaa
     5641 tactttgact tggacacctt tttggatggc atcgaacata aatatctgag tagaaagttc
     5701 catttcgcga aaaccggcca actgataggg acgttcatgg ctttctttat aatattgatg
     5761 tcccaatgcc acagcaactt cttgctgact cgatagctgt gcttcttgat agagtttcgc
     5821 tgctaacgtt tgttcaggat gatccatttg agtcaatgct tcctctacta aataaagaga
     5881 atccaaccaa tccaattcaa ccagcatttg cttcattcct tcaagaattt ctctaccttc
     5941 ttgatgaaat tctgtttgat ctgatggttg ttctagtgct actttgttat ttcgagtcgt
     6001 accttctgct acccattggt cacagtcttc tttttcttct gtccataaca tgtacatcag
     6061 gaataattgt aagaaccgaa cttgctctgg attgataccc aatcttgcat aaggattcaa
     6121 atcgatattc cgcaattcaa tataacgaac acctgttgtt gctaaatctg cgactttctt
     6181 tccgcctcgg aaacgaagcg gtgtataaaa ttctttctcc tccgaaagtt tcccttcttc
     6241 gatcattcgc tcgatatctg ctaaatactg tttcatagaa gcataggaaa ctttcacatt
     6301 tggatgattc gtatagccca gcgcactgtt gcgaatactg cgaacaggtt cttgcggatg
     6361 tgattcatct aaaaagtagt tcttctcact cattggtgaa gcaccgaata aataagtgat
     6421 catccagcga tagcgcataa agtttctcgc tgttttcaaa taaagttctg ttttgaattc
     6481 ggaaaaatct tgaaattctt cctgatggct gaacaatgtt cttagcaact catcgccaaa
     6541 ttcaaaattg aaatgaatac cgcttaccat ctgttttcgc ttcccgtatt cttttgctaa
     6601 ataacgtcga tacaagacat cttcgaaatt ttttaatttt gcaataatga tctcttcgtc
     6661 tttttccggt aaggcaggtg gcatacttaa tggccagatc atctcttctt tcggtataga
     6721 acgagcagtc acatcataaa cagcagcgag atactgaaat aattcgggaa tagaatcggt
     6781 aacaggggtg atcatctctg tttgtgtttc gctaaaatct gtttggatat aggggtgata
     6841 ggatcgatcg ccaaagattg ctggatgatc ggtttttgct agatttcctg caagatcaac
     6901 ccgctgtcct tcccgttcta taccaaatcg agcttggtcg ataaaagggc gcgcattgac
     6961 atgcaataat aattgcttaa aattcatcat taagtgacct ccaattcata tgaatctaat
     7021 tataatcaaa aagtctaaga aaaagaacga aattgtttga gaaccgctta caaataagta
     7081 taaaaatgaa ggcatcaaaa acacagctat gttaaaaaca atcattcaaa caaggagctg
     7141 caacttttgt cacagctcct gctttttaga catgggttat tcttctttta gtaatccttt
     7201 gaacaatcca acgacaaaat catttggatc aaacggctcc aagtcatcga ttccttcacc
     7261 aagaccgacc aatttcactg gtaaatgcaa ttcattacgg atagcaagta cgataccgcc
     7321 ctttgctgta ccatctaatt ttgtcaaaac aagaccggtc acatctgttg tttctttgaa
     7381 ttgttttgct tgaaccatcg cgttttgacc agtggttgca tctaatacaa gaagtgtttc
     7441 ttggggtgct tctggtaatt cccgctggat tactcgtttt attttttcta attcgttcat
     7501 caagttcact ttattttgca gtcggcctgc agtatcaact agtaaaacat ctgcctgctc
     7561 tgtctttgct ctttccaaag catcgaatac gacagctgct ggatcgcctc ctgcattgcc
     7621 acgaacaact tcaacaccag cgcgttcgcc ccagacaact aactggtcga ttgctccagc
     7681 gcggaaggta tctgctgctg ccatcaatac ttttttacct tcgtttttga actgatgggc
     7741 aagtttacca atacttgttg ttttccctac accattaaca ccgacaaaca aaataactgt
     7801 tagtccgtct tcttggatat ttagttcatt tacttcattg attccttccg cttcatagag
     7861 atccacaagt ttctcgatga tcgcattttg gacttctgcc gattttttcg catttctgag
     7921 tttcacttct tgacgtaatg cttcggtgat tcgtatcgct gtttcaaaac ctacatctgc
     7981 accgataagt gtttcttcta attcttcaaa aaagtcttca tcgactgttc gaaaattggc
     8041 aaacaactca ttcatccgtt caccaaatgt tttacgcgtc ttttctaaac ccttgtcgta
     8101 cttctcctgt acttcaactt cttctgtttt ctcttcgcca gtaaaagcac gcttgatttt
     8161 atcaaaaaat cccatttgac tccccctact ctaatacaaa tttttggatc actttggcta
     8221 cgccattttc ttcattgctg gctgtcacga aatctgctgc ttctttgacg aaaggcacag
     8281 cattctccat cgctacgcct agtccagcat attggatcat cggtaaatca ttttcctcat
     8341 caccgatcgc catgatttct tcttgtttca aaccaagatc ttttgccaat aaagaaatac
     8401 cataagcttt tgtaacacct tttggcataa attccaaaag catacttctt gttttgatga
     8461 tctcatacat ttcatggaat tctgctggta tttctttgat tttaggatct aattcttctt
     8521 ggtcataagc gattactgcc ttattaagca caaattcatc tgtaatatcg cttatatccg
     8581 ctggttggaa ttgcagcagc ttattcaaca cattgtacaa ggattgcttc cttggggcac
     8641 taggcagctg taaaaccaca ttgtcagaca aaacatctag tggcagatcc aattgctgag
     8701 ccagtttgta taagcgttgg atatctgata cttccatcac tttcttttcc atgatttcac
     8761 cagtatcatt tttttgtacc aaaccgccgt tgaaagtgat gctgtagtca ccttcatcta
     8821 ccagtcctaa ttcttgcaga taatgcgcca tcgcagctaa ggggcgtcct gtacatagaa
     8881 cgatcttgac cccttgctgt tttgcttgtg ctaatgcttg tttattctca tctgaaatca
     8941 gcttttgttc atttaataat gtaccgtcca aatcgattgc tactaatttg atcattttcg
     9001 ttcctacact ttcgtttcgt tgattttcgc ctcttcattg atatcttcca tccgtacaga
     9061 aacgattttt gatacaccgg attcctgcat cgtcactccg tacagtacat ctgctgcgac
     9121 catcgttcct ttgcggtgtg tgactacgat aaattgtgtg tcatcttgga aatcgctcaa
     9181 gtaacgacca aaacgtttca cgttggcttc atctaacgct gcttctacct catctaagat
     9241 acaaaacgga acaggacaga cacggataat ggaaaagagc aacgcgatgg ctgtaagtgc
     9301 tcgttctcct ccagaaagca aacttagact ttgcagtttt ttgccaggag gttgtacttc
     9361 aatctcaata cctgttttca gcaaatcagt tggttccgtc agtactaatt cagctttacc
     9421 gccgccaaac atatttggaa agacaatttt gaattcttga cggatagctt caaatacttc
     9481 tttgaatctt gtccgcactt catcatccat ctcatccatc gtttcaaaca attggttttt
     9541 cgctgctaac agatcatctc gctgtgttgc taagaatgta tgtctttcac tgacttgttc
     9601 gtattgttcg attgaattta agtttactgg tcccagtttt tcaatctgtt cttttaagga
     9661 tgaaaccttt gttctactgt cttcgatatc ggttgtttct tgatagtctt gggaagcttt
     9721 ctcaaatgtc agctgatatt ctgactgcag ataggtcaaa tgattatcta aaatcatttc
     9781 agctcggtct ttttggactt ctaatttgga ttgttccgac agtcgtgctt tttgctcacg
     9841 attacgctct gccaagactg cttcaagctt atcgatctcc ttttgccttc tatctcgctg
     9901 ctctttggct tttgccaact cggcttttaa tgtttctcgc tgagcagata attcgttaat
     9961 ttgtttttcc aaactttctt ctgtgacttc atgatcagaa aagtctgctg tcaatgtcgc
    10021 caactgcttt tctatcgctt cttgacgttc ggttgcctct gctttttgga ctcgggcacc
    10081 gcggagctgg atctgcagat gattaaactg ctctttcaag acagcaagat ctgcttgttc
    10141 ttgtgctttt tgactttgta cttgtgctct tctggcttcc atctggtcgc tttcttgact
    10201 taacgatttg atttcttcgt cgattttttg tcgttgcgat tcaagatctg tttgtttgtc
    10261 tttcaattcg ttcgtttgct tttcgtaatc ttcgataaac tgctgcagtt cacgtgtctc
    10321 aaaagttgag atttgctttt ctttttcaaa acgttcaagt tcatttatga tgttttgtag
    10381 ctgatttgtc gcttcttgtt cttcaaaacg taattgttcg cctcgtgtac gcaaaacttc
    10441 ttgttcttcg gctaaacgag ctgtttcttg ttgcagctcc tgaacttttt tttcttgtgc
    10501 ttggagttgt ttatctgctt cttcaaattc actagtcaac tgtttcaatt cttgattttg
    10561 tacaaacaaa ctgccttggt ttcctcgttt attcgctcca cctgtcatgg atcctccggc
    10621 attcatcaca tcaccttcta aggaaaccac tcgatattga tagcggatcg tttgcgcgat
    10681 tgcattggca ctagttaagt ctttggctaa aaggatcgtt cctagcaggt tttgaaccac
    10741 agtttggatt tgatcgggat aactgacttg ttcactggca atccctataa acccttctac
    10801 agctgctgct tgtgtcaata tatgagccgg taattgtctt ggtttgattg tcgtcagtgg
    10861 aaggaatgtt gctcttcctc ctctttgctg tttcaaatac gtgatggctt gccgagcatc
    10921 tttttcattt tcaacgatga catgctgggc tgccccacct aaagctgttt cgatagctaa
    10981 cgtgaaatct gcaggaacat cgatcagttc agccactgct ccgacaatac ccgaaagctg
    11041 ctgtttatgc tgcaagacca atcgaacccc ttggtaaaaa ccaaaatagt tttcttggat
    11101 ttcttgaagg cttttcttac gagcacgtag ctgctggact tgattcatta attgatacat
    11161 tttaggctgt tcttttgcta atgcttcttg gaactttttc ccgttttgtt ggatcatttc
    11221 taacttctct tttgtttcag ttaaagatga ttttagctgc gcctgctttt gtgtcaattc
    11281 ttctttttgt aagatcaatc ggtctactga tgcttctact tccgattgct tggctaacgt
    11341 ctgcttgctt tttgctgttt cttgtatata ttgacgttct agatacttca gttcgtttcc
    11401 tacagctgct tgttcttgca tcaaatcaac atattgatct cttaattctg ccaataattc
    11461 tttcgttgat ttttggtatt tttctaattc ttgctgtgtt tttatgagat ttgcttcagc
    11521 tttttggatc tctgtttctt tttctgctgc tgctttcatc aagctttctt gaagcttttc
    11581 aaaatgttta accttttttt gtgcttctgc taatgaagtt tgatattctt gcgagctttt
    11641 ttgtgtatgc tttgtccgct cctgcaaaac atctttttgt ccttctgtct gcttcagttt
    11701 ttccgaaaga tctaataata cttgctgatt tttttcgatc agacgatcgg cttgcgcatt
    11761 ttctttacgc tgcttcgcca aaatggattc ttgttcttgg atcgactcag atagtttgcc
    11821 taattctaga ttgaatttcg caagctgtgc ttgtttgttg tcccaatctt tttttgctgt
    11881 cttgatttca gcaaccatca aagagacatc ggtttgagtc agtgtttctt ttagccgtaa
    11941 aaactccttt gctgcctcac tctgggcggc caaaggagtt aactgttctt ccaattcgtg
    12001 gatgatgtct tggacgcggc tgaggttgtc ttctgtttca aaaagcttct gttctgcttt
    12061 ctttttccgc tgcttgtatt tcaagacacc agctgcttct tcaaagatcc cacgacggtc
    12121 ttccggttta ctactgaaaa tcgcttctac ttttccttga gaaatgattg agaacgattc
    12181 ttttcctaat ccagaatcta agaacaattc ttggatatct tttaatctgc agctttgttt
    12241 attgatgaaa aactcgcttt ccccagtccg tcgataacgg cgggttacac tgatctcatt
    12301 gtactccaat ggcagataat gatcactatt gtctaaaatc actgtgactt ctgcaatatt
    12361 cagctgctta cgggtatcgg aaccggcaaa gatgatatcc ggcatttttc cgcctcgcag
    12421 acttttagca gattgttctc ctaataccca gcggatagcc tctgtaatat tgctttttcc
    12481 gctgccattt ggtccaacca ctgctgtgac gctattttca aaatcaatga ctgtcttgtc
    12541 tgcaaatgat ttaaaaccgg ctatttcaat tcgtttcaaa tacaaagctt tccctcactt
    12601 acttttacag tgctgccaat gcattttccg ccgcagcttg ttctgctaat tttttcgatt
    12661 tcccttgtcc agtgccaatc aattgatcat ctacataaac ttcgatccaa aatactcgtt
    12721 catgagctgg accttcttca ttgatcaatc ggtattcgat tgatacatca cctgattttt
    12781 gcaagacttc ttgtagtttt gttttatgat ccatctcatg tgaaaaagca cctgctttga
    12841 ctttaggaaa aatcactttt tcgatgaatg catgggtcgt gtcgaatccc tggtcaagat
    12901 ataacgcgcc taaaaaagct tcgaataggt cgcaaagcaa tgctggtcgc gtacgtcctc
    12961 ctgaattctc ttctcccttg cccaacaaaa catattgatc aaagtgacat tctttggcaa
    13021 atttcgacaa gctgtcttcg cgaacgatcg cagctcgtgt ttttgtcaat tttccttctg
    13081 gcaaatgagg atataaacgg aataaatact gagaaaccat cagttctaaa acggcatctc
    13141 ctaaaaattc aagtcgctca ttatccgata attgcagatt gcgatgctca ttcacatagg
    13201 atgaatgggt aaaagcttgt tcaagcaaat ttagatcgtg aaaaacgatg ttatagtttt
    13261 cttttagttc ttttgtcagc tggttgtcca ttttccacat tccttttatg gatatttttt
    13321 cgatacttcc ttattatact ggttttttgt caaatttgaa atggttcggt caaaaggtaa
    13381 gtaaagcttt aataatccta gtgattgacg agaagttgtg aaaaattcgt ttagtttcga
    13441 gtatgaagaa aaaattttgt gaaaatggct ttccatattt ttgcataaat ttgacttaat
    13501 accgagaaac tggactttga acaccgttta ttggagaagt catgaaatca gcgccttgac
    13561 ctgaatatta gagaaataaa cagaaaaata tcaaaaaacc gaaaaaggac atcgcccttt
    13621 ttcggtcatt tttagtgatt atgaacgtga atctgctgta acagaaacat ttgcccatgg
    13681 gtttggtaca tcataagacc aatcccagtc tttgacacgg tcgttgactg gcaatacttc
    13741 attacggaac aacgttggaa tcgcaaatgc ttcttcaaat gcatattgtt gccatttttg
    13801 atatgcttct ttttggtact cttcatcaaa tgctttgctt gaactgatat cgttgatcaa
    13861 ttttgtattt tcttctgatt cataacgagt gtagttgaac gcagcatttg gtccccatag
    13921 tccacttgga tttggatcag ttcctgtatt ccaagcagct tggtaaacat caatttcagg
    13981 atcatcgttt tctaatttat catagaaaga attgaattcg atcagacgac ctgttgtgta
    14041 ttggacatcc aaaccaattt ctttccattg ttggatatag taatctgcca atggttgcgc
    14101 tgtttcccca ccggacatcg aagcaaagtt gattgttagt ttatttccat ctttgtcttc
    14161 tcttaaacca tcgtcatcca catctttgta gccagcatca tctaatagtt ttttcgcttt
    14221 gtctagatct tgtgtgaatc ctttcatctc atcatcatgg tatgactgga atactggagg
    14281 aatcaatgaa gtcgcattgc ttctcaaacc tgcatagaaa cgttcaccta ctgcattatt
    14341 atctaatgca tagcccattg cttgacgtaa tgatttatca gccatttttg aatctggatt
    14401 gtattccaca ctctttgtat ctgcattcca tttacctaac ttgaatccta gataagtata
    14461 agaagtttgt tcacggccta agtttgtata gccagcaatt tctttatagg tatcgtaagt
    14521 atctgttggc atttggaaaa tcatatcata taatttagag ttcaatgctt cagttgcact
    14581 tgccatcggt acagatttca tcgtgatctt tttcaattta ggcgtaccat tccagtagtg
    14641 ctcgtttggc aagtattcta ctgattctcc tgcaacgata ttactcatgt aataagggcc
    14701 aaaagtcact ggatttttac gtacttgatc gcttgattcc atatctttga ttgctacacc
    14761 ttctaacgta tgttttggtg ctgcatatga ccagatacca ccacccgatt gaagcatgct
    14821 tgggtttact tctttatatt cgatttcaac tgttttgtcg tctatttttt tgattccgga
    14881 gattgtgtcg gcacttccat catgatattc ttccatacct acgatgttac ggaaagtgtc
    14941 gtcataacgg ataccagtgt aatctttatt cccaatgatt tcgtaaggga aaatcacatc
    15001 gtccgccgtt acatcttcac catctgacca tttgacgttg tcttttactt tgattgtcgc
    15061 ttttttcgta tcttcatcta gatcaagcgt tgcagctccg tcgtctgtga ttctgaaatc
    15121 gtcatcgttt gcaaatagcg gttcatgaga aggagccata aaagctgcat catatgaatc
    15181 ttggtaaaac tcccactgga acaatccttt aaactgactg tccgttgcga ccgcagcttc
    15241 taattcgccg tcttcgattg catcatcatt attttttgtt gcaattggaa aagttaattc
    15301 ctctttgtta ttgccgttgc ctccagagtt gtttccagag gtatctgtac ctccgccccc
    15361 gcatgctgca agaacgacag cagacatcag cgctgcaaat ccaaaaatcg ttttcttcat
    15421 tcctatttcc cccttcgctt ctatactagc ctaaccgttg gcgcgcatct gcagaacgct
    15481 tgaatgcttg tccaacataa ttgatactca acatcaaaac taaaataagg attgatgcgg
    15541 gtaaccagat ccattgccga ttcactaaga cttctccgtt acttgcataa ccgatcaatg
    15601 tacctaaact aggtacattt gccggcaagc cgaaacctaa gaaagtcaaa gttgtctcga
    15661 taccgatgtt tgccgcaaag ttcatcgtca aattagtaat gattaatgaa cttagattcg
    15721 gcatgatttc gcggaacatg attttaaaat cattggttcc cattgttttt gaggcactga
    15781 cataatctct tcttgcttca gataatgtct tgcttcggaa taatcgggct ttagctaccc
    15841 aataaaatgc actcatgatg aatataaagg accagacatt gtatctggaa ataatcgata
    15901 cgaagacgat aatgatcagc atgatcggta gaatcatgat aaaatcaacg atccgcatca
    15961 agatgttgtc aatgatccct ccgtagtagc cagagatgat ccctaatcca accccgatga
    16021 tcgaagtaag gatcgtgatc gcaaatccga tcgtaacaga gtttctagca cctatgatca
    16081 attgtcctaa tacatcacgt ccgccttcat ctgctcccag aatgaacttt tgtccttgtg
    16141 tcgaaactgt tcctggtgca gcgtatttgt cgaagatact aacatacatc actttgtctt
    16201 gatcggtcag catcgcaccg ataaagatca ctaataataa aaccactagc aaacctaatg
    16261 aaaaaatagc aattttatct tttttaaatt ctcgagcaat cattcgtatc cccataggag
    16321 gaatactttc ttgaactgct gtattgtttt tttcactcat cgatccattc tcctttctga
    16381 ttggttttat tgaatccgaa tacgcgggtc aacgatactc atgatgatat ctgaaagtaa
    16441 tgtccccact agcgtcgcga ttcctaaaat caatactaag gatgtgatca cggtatagtc
    16501 ccgttggccg atggaatcaa taaatagttt tccgactccc ggataaccaa aaatcttttc
    16561 gataactaca gaaccagcaa tcaacgaagt gatttcatac cccatttgag aagccatcgg
    16621 caacgctgca tttcggaaaa tatgtcgact gaatacttta ttcgtcggta cccctttgga
    16681 acgggctgtt ctgacaaaat cgagcgattt cgaatcgatt acttcacttc gcagatattg
    16741 gattgtgacg gcagtcgcca aaaatgcttg ggtaattgat ggtaacagaa tatatttcaa
    16801 acggtctatc cattgagtga taaatccacc ttgcaaacct agtgtttgtg atccacttgt
    16861 tgggaaccag cctaaacgat aaccaaaaac aaatagcatg atcaagccga agataaataa
    16921 aggaacagca aaactaaaga agttgtagat tacgacaatc ttatccaaga ttgaattttg
    16981 ataccggcca gcaagcatcc ccagaggcac agcaatcaaa taagtaagga caactgtcaa
    17041 gatcgataga tataaagtgt tgatgatccg ttcgccgatc aagtcagcaa cagagcgttt
    17101 gtataagaaa ctttgaccaa aatcgccttg caaagcattt ccgatccaac ggaagtactg
    17161 gacataccat gggtcattca agccagctgc ttgtcgcatc tgttcgatca ctgccggatc
    17221 ctgatttgga ttgatcaaac cggtaaatgg atcccccggc atcgcttggg ctaataagaa
    17281 gatcagaaca cttaaaataa taacttgcgg gatcatcaat aaaacacgtc gcagaatcgt
    17341 tttccacatt agcttgcacc tccatctttc aacgccactt tgtgtgtagt tgtgatcgta
    17401 cgcaaatcgt agactcgacc agtcgcatca tagtattctt tttggttttg gatatactct
    17461 tgttctactc ttcgtcgatt ttctttgtgg ctttccctat tttctacatc gatttgcgga
    17521 atagctgaaa gcagacgttt cgtatagata tgtcgcgggt cattataaat gtcttctctt
    17581 gtaccgattt ctacaaatcg acctttgtac ataattgcga tattgtcgca catgtgtttt
    17641 actacaccta aatcatgtga aatgaataaa tagctgaggc cgtattcttg ctggattcgt
    17701 ttcatgaaat tcaatacttg cgcttgaaca gatagatcca gagcagatac aggttcatcg
    17761 gctacgatca gttttggatt ggtagcaaca gcacgggcta ctcctagacg ttgacgttgt
    17821 ccgcctgaaa attcatgagg atatttatat agcgcatctt ccggcatccc aacgatatct
    17881 agcagtcctt ttaccttttt cttttcttct tgatcgctta accgttcaaa atttcggatg
    17941 ggttcagcaa tgatatccaa cactcgcttt ttaggattca aactggacat tgaatcttgg
    18001 aagatcatct gcacatcctt gttataaccc atttttttgc ggttgcttcg cttcgttaca
    18061 tcttttcctt catacatgat ttgtccggct gttacctttt ctaatcccac aacggctttt
    18121 cccgtagtag attttccaga gccagactct cccactaaac catatgtctt tcctttttcg
    18181 ataataaaat ccacaccatc tacagcatat acatgatccg ttactcgatt gaagaatcca
    18241 ctgcgaatag ggtaatgaac tttcaggtct ttgattgtga ttaattctgt catctacacc
    18301 tctccttttt cgtctctaaa gtggaaatgc ttatagcagg agcaacgtac caagtggttc
    18361 ggtgcgactt catgaaggat aggctcttct tcatgatcgc ttgctggtat ccatggaata
    18421 cgtggtgcaa accgacaacc tttacgaggc atgtttttca gagaaggaac gacaccttcg
    18481 atcacatgga gttctgcatc atgatttcct tcttgtggta tagagttcag taaagagcgt
    18541 gtataaggat gcttaggatg atcaaacagt tcttttacac ctgctacttc aacgaactgt
    18601 ccgccataca tcactgccac tcgatcagcc atttctgcta cgactcctaa gtcatgagtg
    18661 atcaagatga tccctgtttt tgtttcttcc tgaaggtctt tcagtaaatc aagaatctgt
    18721 gcttggatgg ttacatctag ggctgtcgtc ggttcatctg caatgacgat tggaggtttg
    18781 caggcgattg cgattgcgat gatgacacgt tgtctcatac cgcctgatag ttcatgggga
    18841 tattggcgtc caactctcgc aggattagga atccctactt gttctagcaa ttctagtgca
    18901 cgagcttgac gctgttctgc attcatcttt gtgtgatagg aaagtccttc tttgatctgg
    18961 tcttcgattc tcatgagagg atttaatgca gacaacggat cttggaaaat cattccgata
    19021 tcatttccgc ggattttatt atagagcgtt tcgttcaggt cgattaaatt cagatcgtta
    19081 tataaaatct cacctgtaat ttttgtgtta ttaggatcaa gtaatcccat aatcgtagta
    19141 gccaaggtac ttttcccgca cccagattcc ccaacgattg ctaaaatctc gtttttgtct
    19201 aaagtaaatg aaacgtcatc tactgcatcg taatagtcat ctttgagtcg gaatcctgta
    19261 tgtaggttct gaacttctaa taattggtta tcggacaatc gaatacccct ctctgcttcc
    19321 taaatggaag tttttcttaa taaaaaagga cattacacag aataaatcgt tttataacaa
    19381 aggttattct tatctttgtg taatgaatta ttaataatta tatagaaaac cgctttactt
    19441 tgcaacataa tttctgttta tcgccagaat tatcagaaaa atgactttta cgtgtcaaaa
    19501 agattaaaaa aagaaggata aaaagctgca tgcagctttt tatccttctt tgcttttatt
    19561 tgaaattatt taagatggct gctgatataa tctactgcgc caccgactgt ttcaatttgt
    19621 tcagcgtctt catctgaaat ttccgtaccg aattcatctt caagctctaa aacgaattcc
    19681 ataatactaa ttgaatcagc ttttaaatca tctttgatgt tcaattcatc cgtcacttta
    19741 tcggtatcta tctcaaaatg atttgaaata attttcgcta ctttgttaaa tacttcttcg
    19801 cgagtcaact ttttcacctc caagaatcat acacttgtca attacctaca ccatggctaa
    19861 ttttactggc atttttcagg aatgtccacc tttttttcaa tggaacggat cgcttcagat
    19921 atccaaatac tattcttcgc ttttttcaaa ttgttcgact aattgtccaa ctacatctgt
    19981 ctcaagcatt gtgtggattt ggcggatcgt atagcgaaca gcatcggggc ctgttgctcc
    20041 atgagtttta atgactggtg ctttcaagcc aaacaaaacg gcaccgccat gtttggaata
    20101 atccatttct gctttcaaag aacgtaagcc gtcttttaaa agcatagcgc ccatttttcc
    20161 tttgatccct tcatttagaa tagcagattt taatagattc atcatattca ttgctgttcc
    20221 ttcaatagat ttcaaaacag cgttgcctgt aaatccatct gtgaccacta catcagccac
    20281 accattgagt agctcacgag cttctacgtt tccaataaaa ttcaagcttg tttcattctg
    20341 aaggagttca taggcttttt tcgtcacttc actacctttt gttgcttctg ttccgttgtt
    20401 taagagtgcg acacgaggac gtccgatacc tcggacttta cgtgcataaa aagaacctaa
    20461 aataccgtat tgcaacaaat gttctggttt gttttctgcg ttagcgccaa gatccagcat
    20521 atcgaaaccg gcaccttctt tccctactac tggcaaggta gacatcaagc cagggcgttc
    20581 gatatttttg atacgtccta cgatgaacaa ccctgctgct agtagcgcgc ctgtattccc
    20641 tgctgaaaat acagcatctg cttcaccgtt tttcactgct tgtgcagcaa ggaccattga
    20701 tgcagtcttt tttcgtcgaa ttgcttttac tggctcatca tcactattga ttttttcgtc
    20761 tgtatggata atcgtgatgt ttttttcatt tgttaagtat ttcttgattt ctgcttcttt
    20821 tccgtaaagt aagaattcta cttctggaaa atcttgctgc gctaaagcca cgccttctac
    20881 aattgcttga ggtgcgtgat ctccgcccat tgcatctacg gcaattttca ttgtttccac
    20941 tcctttttcc gtcatcagta taaagtatag cacagtctgc taatcgaaat aacgattatc
    21001 ctgattttct atttggaggc tttgcgctaa aggctgatac tcaggaagca tctgccaatc
    21061 ctttatttgc caaagggctg cagcctcttc tctggctacc tccaaaacat ttccatccgt
    21121 caccaagtca gcaatcgcaa attgaggcag accagattga cggaagccaa atacttcacc
    21181 aggaccacgt aattccagat ctttttcgct tagtacaaaa ccattggtgg tctctgtcat
    21241 gattttcatc cgttccacac ctagttcatt tttaggatta gcaactaaga tacagtagga
    21301 agcatcactt ccgcgtccaa cacgtccacg aagttggtgc agctgtgcca atccaaatcg
    21361 atccgcatcc atgatcagca tgacggtcgc gttcggcacg tttaccccta cttcaattac
    21421 agtagtcgaa actaatatct gcatctggtt gtctttgaag gcttccatga ttacttcttt
    21481 ttcctgattc ttcattttac catgtaacaa gccaacttca tattttggtg caaatagttc
    21541 acgaagtttt tcatagattt ccactgcatt ttttacatct aatgcttctg attcttcaat
    21601 cagcggacag atgacataca tttgatgtcc tcttgccagt tcttttttcg tccaatccag
    21661 tacactgtcc aattgaggtg ttcttaccca gcgtgtttcg atcgggatac gtccagctgg
    21721 aagttcatca atgatagata catccatctc accatatgct gtaatagcta atgttctagg
    21781 aatcggagtc gctgtcataa ataagacatc tggtcgccag cctttttcac gaagtacttt
    21841 ccgctggttc acgccaaaac ggtgctgctc atccgtaata actagaccta aattctgaaa
    21901 ttcgactcca tcttggatca atgcatgtgt gccgatcacg acgttaattt ccccttgctc
    21961 tagtttttca aggagctcac gtcgttcttt tgctttcgtt gaccccgtaa tcaatgcgat
    22021 ggttacttca ttgggatcaa acagctgttg caagctttcc atatgttgtt gtgccaagat
    22081 ttctgtaggc accatcaacg caccttggaa acctgctgta acggtagcgt ataacgcaat
    22141 cgcggcaacc actgtttttc cgctacctac atctccctgc aaaagtcgct gcatatgctg
    22201 cggactgcga agatcccgac agatttcatt cgttacacgt ttttgtgcat ttgtcagttc
    22261 aaaaggtagc ttttgagtaa attgtttcaa tttttggaca tcataaagga tctccaaacc
    22321 attcgtttct gctttttctg cttttttcaa tccttgaaga cgcatttgaa ataaaaagaa
    22381 ctcttcaaaa acgacacgtc gtttggcttg atggttttct tcgtggtctt ttggaaaatg
    22441 catacttctt acagcagaag ctcggtcaag cagtcgatat ttctccacaa ggttgtttgg
    22501 cagattttct tcaatcaatg tgccgtattc ttgaaaccct tttttgatta gatcaactaa
    22561 tgtcgtttga cgaattgatt tgttgacgtg atatatcggc gaaaagtcat ccattgtttg
    22621 agaacctaaa atcttcatcc cgttcaaggc tttacgcttt gcatcccatt taccatatac
    22681 ggcgatttcc tccgataaaa tgactttttc ttttagataa ggttgattaa aaaaagaaac
    22741 gttgaacaca tcatgttctt gcatcatacg aaattgcaat cggctttttt tataaccaaa
    22801 tcgattcatc acaggaggcg agactacgat tcctttcaaa gtaacctttt cttgatcctg
    22861 gatctcgttt agatttcttt cctgaatatc ttcgtagcga aaaggataat aagtcagtaa
    22921 gtcttcaatc gtttggatgc ccaagctggc aagactagct gctctttttt ctccgactcc
    22981 tgccagcata gtgattggat catctaaaga acgcataaat ttttcctccc tcctttaaca
    23041 aaaaaaggac ttggtcagcg atcatacctg atcactaacc gaaggccttt tatgaatgaa
    23101 tactgtgtta gaaagatatt ctgacacaga aattattcag cagaaaatag ataaggatag
    23161 acaggctgat ctccttggtg gatctcgatt tccaaatcag gatgtttttc tgttagtttc
    23221 tcagcaaatt catttgcttg ttcaactgtt ccttcatcac ctgccaagat cgttacgatc
    23281 tctgtgtcct cattgatcat ttgttctagt gtacttaacg ctgttgctgt gatatctggg
    23341 ttagagatca ggatttttcc atcgatcatg cccaaaaagt ctccttcttt gatctctaca
    23401 ccgtcaattg aggtatctct gatcgcgtga gtgacttgac cgctcacaac gctagacaac
    23461 atttctgtca tcgtttcttt gttttcttct aaagaagctt gatcattgaa tgaaagcata
    23521 gctgtcaatc cttgggaaat cgttcttgaa ggaacaactg ctactgggat atctgctact
    23581 tctgctgctt gatcagctgc catgaagatg ttcttattgt ttggcaagac aatcacttta
    23641 tctgcgttta cttcttgaat cgctttaaca atgtcttccg tacttggatt cattgtttgt
    23701 ccaccactga taacataatt agcgcctaaa ctttcaaaca gctcttggac gcctttacca
    23761 gcagcgatcg ctacgatacc atacgggata cgtggtattt cttttttcgc aaactcgttc
    23821 acttcttcat cgtgttccaa aatcgtctca tgctgcaagc gcatgttgtc gactttgatt
    23881 ttgactaatg aaccgaattt ttgtccataa ttcaagactt ctcctgggtt ttctgtatgc
    23941 acgtggactt tgatgatctc gtcatcattg accacaagta aagaatctcc tataccatct
    24001 aaatagttac ggaattcttc gtaatcgaat gtactatcga ctgtaggtcc ttctccgatt
    24061 tttaccatga tttctgtaca gtaaccaaaa tggatatctt ctgtcgccat ttgaccttgg
    24121 acactgcgat gatgttctgc attgaccata tcatccattt ctgctggaga aggttcatat
    24181 gtttcatccg cttggaattc tccattcaat gcacttaaaa agccctcata aacaaacagc
    24241 agtccttgcc ctccactgtc tacgacaccg acttctttta ggactggaag caagtctgga
    24301 gttttagcta aagcacgctt tgctcctttt acaacagctg tcattacttc aacgacatca
    24361 tcagtacttt ttgctttctt ttctccagct ttcgctgctt ctctagctac tgtcaaaatc
    24421 gttccttcta ctggtttcat tactgcttta tacgcagttt caactccatg agtgaaggct
    24481 gcagcaagat cagaagcatt taatgagaca acatctggaa tttgcttaga aaatccacga
    24541 aacaattgag acaaaataac gccggaattt ccgcgcgcac ccatcagcaa tccttttgaa
    24601 agcattgctg ctaattctcc tactttttct gaggttgaat ctactactgc cttagctcca
    24661 ctagtcatag atagattcat atttgttccc gtatcgccat ctggcacagg gaatacattt
    24721 aatgaattga cgtattctgc atttacttgc agacgattcg cacctgcctg gaccatttcc
    24781 tggaactggc ttgcacttat ttctgttaca ctcacctaga taatcctcct tcagatcgac
    24841 tgctgagtta ccagccagtc tcctgcctag tctggcagta cacgaacacc ttgtacaaag
    24901 acattaactg aattggcagt tacgcctaat aatgcctcta gattgtattt cactttttct
    24961 tggacgttgc gggaaacttc agagattttc gtaccatagc tgataatcgt ataaacatct
    25021 acagcaatac cattatcttc ttggcggaca actactcctc ttgagtaatt ttctttacgc
    25081 aaaatctcag taatattatc tttaatttga tttttgctgg ccattcctac aacaccgaac
    25141 acatctgtcg cggcgccacc aacaactgtt gcaattacat cattggtaat ttcaatggtt
    25201 ccagctgacg tttgaatttt tacagccatg aatgagcctc cttaacgggt aaaaaactcc
    25261 catttttctt ctatcatatt ttatcatagc gcaacttgaa ataaaaggca gttacccaaa
    25321 gtactcaaaa accttgcaaa atttcaggta taaccttgtt taatttacaa ttattcttct
    25381 caattgtcaa ttgtatctat gaaaatgtac ttgcaaattc ccattctata tgatatgtta
    25441 gtctggtatg agctgaatta ttctattgct ccaaatcaag gcaaaggagg aaattaccat
    25501 ggcaaaagtt tgttacttta caggtcgcaa gacaaaaagt ggaaacaacc gttcacacgc
    25561 tatgaactca acaaaacgta ctgttaaacc taacttacaa aaagttcgcg ttatggtcga
    25621 cggaaaacct aaaaaagttt gggtgtcaac tcgtgctttg aaatcaggaa aagttgaacg
    25681 cgtttaattg aatgatactt ggaccgttca gatagtgaac ggttttttat ttaataaaaa
    25741 tttttaaggg tgggactttt gattcctacc ctttttttgc gtttattttt gttatacgaa
    25801 caaattatga agttatttat tctaaatttt atttttttgt gaaaactaga ctctttttaa
    25861 gcagagaaaa acaaggatat gataaactga atacaacttc atagaaattt ggtggaaaat
    25921 aaatatgaaa aaaaatttaa agtcattctt tagagaaaac ggacgctacg cagcagctag
    25981 tttcctgatt ccgtttttga ttttagcact tgtttatcta agtatcggaa tctatcctgg
    26041 aagcagcaga agtattttgg caagtgatgc cttttcacag ttttctaatt ttcatgccag
    26101 cttccgaaat gttcttcttg gaaagcaaag tattttttat acatggaatg cttctctagg
    26161 attgaattat ttatctttaa tttcttacta tctaggcggg atcttcacgc cactggtgtt
    26221 cttttttcca aaccagatga tgccagatgc actgtatgtt ctgactttgc ttaaggtcgg
    26281 ctgtgccgga ttggcttttt ggtttcttgc acgtacgttc aagattcctc gctgggggca
    26341 tgttgcactt agcgtcagtt acgcattgat ctcatttatc actgcacatt cggagatcat
    26401 catgtggctg gatgcatttg tttatcttcc attagtgatt ttggggattc atcgtgttat
    26461 ggatcttaaa cggccgaaac ttttatttat cagttattta ctcttgtttt tatccagttt
    26521 ttatatgggt tttatgattg gcgtattttc tttcttatat tttatcgccc gtctattgac
    26581 aaactggtca gaatataaaa aaacgattgt tccttatggt atcacttctt tactggcagg
    26641 cggcgcttca atgatcattg tattgccagc agtgctggat cttcggtcaa atggcgaggc
    26701 actgagtcag atcactacgt tgaaaacaga agcgacttct tatctggatc taatcatgaa
    26761 aaatatgatt ggtgtttatg acacgacaaa atacggatcg attccgttcg tttacgcagg
    26821 tctgctacca ttagtcctct gtctgtttta ttttgccagt cggaaagtac cgttgaaaaa
    26881 taaatttttg tacggcagtc ttttcgccat acttatcgca agtttttatc ttgttccatt
    26941 gaatttgttc tggcatggta tgcatgcacc gaatatgttt ttattccgtt atgcctatct
    27001 gttctctttc ttagtgctcc tgcttgcagg aaaaggatgg gaaaagatag aacaagacga
    27061 tcgtgggctg ttgttaggaa tcattttggt cgtagctgct ctctttgcgg tggcatgggg
    27121 catcaaacct gaaggaagct acacctatgt gacgatgact tcctttgtat tgacagtggt
    27181 tttcttaggg atttatgctt ttgctgtggg attttatcag cataaaaaac tttcattcaa
    27241 tcatctgagt atattgttat tattgatcat gtctgctgaa gcactggtca atacgaacag
    27301 tatgatccat ggcattcttg atgattggaa ttatgcttcc cgcagtttat atactgatcc
    27361 ttatcctgac ttgaaagctc ttgtaaatga aacaaaaaaa gattccgact cgttttaccg
    27421 cttggaaaat ctggatccag tatctgcaaa cgattcgatc aactatggtt acagtggtat
    27481 cagtttgttt tcttctataa gaaatcgtaa ttcttcctct tacctggata aattaggttt
    27541 ccgttcaaga gggacaaatt tgaatatccg ttataacaat aatactttgc tgatggatgg
    27601 gtttacagga atcaaatata acatttctaa aaacgaaagc acgttcagta aatatggctt
    27661 ccgccaagct ggtcaaagcg gcgactatac actgtatcag aatgtcaatg cattgccact
    27721 agcatttact gcaccgttgt ccgttaatga tgttgagcag ccagtgacag ataatttgac
    27781 cagtcagaca aacttgatca atacattgtc tggtctaaat gaacagtatt ttacattcta
    27841 tacacctact ttgaagaatc agttgaatac gacgatcaca aagacaacga ctggcgtcac
    27901 ttactcagaa acagcagata accagccaaa agagctcact tgggaagtcc atgtgcctgc
    27961 aaatacacaa gcttacttaa gtttgttccc aaccaatttt gcgcaattag aaagttctag
    28021 tgcgacgatc actgtcaatg gtacatcgcg aaaaacgcaa atcaacattt ctggtcaata
    28081 ctatgatctt ggctattatc ctcaagagac aacagtgaca tttactgcaa gtttttatgg
    28141 gacgaaagaa ataagtttca tggagcctaa agtcgttggt ctagatgttg tagcctatca
    28201 agccgcaatg aatcagatca aagaaaaagg tgtcgaaatg gaagccactg gcagaaaagc
    28261 ttctgggact gtaaataccg ctgaagattc catgttggtg acaactattc cttatgacaa
    28321 aggatggaaa gctaaaattg atgggaaacc agtcaaggtc agcaatttca aagacgcatt
    28381 cttgatgata aaagttccag caggtaaaca tactgtcact ttcagttact tgcctgaagg
    28441 atttacgata ggtgcaatcc tttttgtcct atgtattgca ttgtttattc tttatgtctg
    28501 gtaccttgac cgcaatcgcc ccttttatgg cacgattgat ccagcaatag aagaacaacc
    28561 acgaaacaca cacagaagaa gacgtagaaa ataaagaaac gatggaacct gttcagactg
    28621 tccgcaccaa gggcaatcaa aacaacgttc catcgttttt tggttacaga aaaaataggt
    28681 aacaaaaaga tgattcactt atcaacgcct cgtccacaag tcacgatatt catcaaagat
    28741 gagaagctgt tttttgaaat tgctctttgt gactcagagt taaatgacag gctcacgacc
    28801 tctttcttaa acggtgttca atcagctgac cttcggtatt agctcaacaa acggcaaaat
    28861 atgagga
//
LOCUS       DACEPW010000014        28546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEPW010000014 DACEPW010000000
VERSION     DACEPW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.014"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            227..715
                     /gene="purE"
                     /locus_tag="IW819_000487"
     CDS             227..715
                     /gene="purE"
                     /locus_tag="IW819_000487"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1243617.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            699..1832
                     /gene="purK"
                     /locus_tag="IW819_000488"
     CDS             699..1832
                     /gene="purK"
                     /locus_tag="IW819_000488"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1243618.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1845..3140
                     /gene="purB"
                     /locus_tag="IW819_000489"
     CDS             1845..3140
                     /gene="purB"
                     /locus_tag="IW819_000489"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1243619.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3320..3697)
                     /locus_tag="IW819_000490"
     CDS             complement(3320..3697)
                     /locus_tag="IW819_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243620.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            3953..4681
                     /locus_tag="IW819_000491"
     CDS             3953..4681
                     /locus_tag="IW819_000491"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1243621.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            4681..4935
                     /gene="purS"
                     /locus_tag="IW819_000492"
     CDS             4681..4935
                     /gene="purS"
                     /locus_tag="IW819_000492"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1243622.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            4937..5608
                     /gene="purQ"
                     /locus_tag="IW819_000493"
     CDS             4937..5608
                     /gene="purQ"
                     /locus_tag="IW819_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1243623.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            5609..7831
                     /gene="purL"
                     /locus_tag="IW819_000494"
     CDS             5609..7831
                     /gene="purL"
                     /locus_tag="IW819_000494"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1243624.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            7816..9255
                     /locus_tag="IW819_000495"
     CDS             7816..9255
                     /locus_tag="IW819_000495"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1243625.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            9287..10330
                     /locus_tag="IW819_000496"
     CDS             9287..10330
                     /locus_tag="IW819_000496"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1243626.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            10327..10905
                     /gene="purN"
                     /locus_tag="IW819_000497"
     CDS             10327..10905
                     /gene="purN"
                     /locus_tag="IW819_000497"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1243627.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            10902..12437
                     /gene="purH"
                     /locus_tag="IW819_000498"
     CDS             10902..12437
                     /gene="purH"
                     /locus_tag="IW819_000498"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1243628.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            12453..13727
                     /gene="purD"
                     /locus_tag="IW819_000499"
     CDS             12453..13727
                     /gene="purD"
                     /locus_tag="IW819_000499"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1243629.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            13849..14763
                     /locus_tag="IW819_000500"
     CDS             13849..14763
                     /locus_tag="IW819_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1243630.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(14896..16461)
                     /locus_tag="IW819_000501"
     CDS             complement(14896..16461)
                     /locus_tag="IW819_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1243631.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(16454..17905)
                     /locus_tag="IW819_000502"
     CDS             complement(16454..17905)
                     /locus_tag="IW819_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1243632.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(17919..18935)
                     /locus_tag="IW819_000503"
     CDS             complement(17919..18935)
                     /locus_tag="IW819_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1243633.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            19155..20243
                     /locus_tag="IW819_000504"
     CDS             19155..20243
                     /locus_tag="IW819_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1243634.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            20297..21823
                     /locus_tag="IW819_000505"
     CDS             20297..21823
                     /locus_tag="IW819_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1243635.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            21825..23030
                     /locus_tag="IW819_000506"
     CDS             21825..23030
                     /locus_tag="IW819_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1243636.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            23287..24264
                     /gene="guaC"
                     /locus_tag="IW819_000507"
     CDS             23287..24264
                     /gene="guaC"
                     /locus_tag="IW819_000507"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1243637.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            24528..25952
                     /locus_tag="IW819_000508"
     CDS             24528..25952
                     /locus_tag="IW819_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1243638.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            26413..27522
                     /gene="pdhA"
                     /locus_tag="IW819_000509"
     CDS             26413..27522
                     /gene="pdhA"
                     /locus_tag="IW819_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1243639.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            27525..28502
                     /locus_tag="IW819_000510"
     CDS             27525..28502
                     /locus_tag="IW819_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1243640.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         9222 a         4877 c         6446 g         8001 t
ORIGIN      
        1 agcttctcat gtttgttgaa tattgttcct tgtgaccgag gagttggcag gagaacaccg
       61 tttatccata aaaaggaatg tgacctaact tttgtcacat tccttttttt tatctttaat
      121 aaacacgaac aataaaatgt ttttctttgt taacattcga aatttctttg acatcaaaaa
      181 aagtgattgt taaaatatga atgtagaaaa actgggagga aaaacaatga cagcaaaaat
      241 ctctgtgatt atggggagta cttcagattg ggaaacaatg aaagaagcat gtcaaatatt
      301 ggatgaattc ggtgtgtctt atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt
      361 gatgtttgaa tatgcacaaa acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc
      421 agggggagcc gcgcatctgc cgggtatggt cgcatcaaaa acaactttgc cggttatcgg
      481 tgtaccggtc caatcacgaa cattaaatgg tttggattcg cttctttcga ttgtccaaat
      541 gcctggagga gtacctgtag ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg
      601 actacttgcg atacagatgc tttccatgta tgatttagaa ttggaggcta aattggctga
      661 aagacgttca atgttggcta aaactgttat agaaagtagt gatcaacttg gttaaacctt
      721 tgttgcctgg ttcaacgatc gggatcgtcg gtggcggtca attaggtcgt atgatggcga
      781 ttagtgcaaa agaaatgggc tttaaagtag gtgtgctgga tcctgtgagc gattgtccag
      841 ccgcacaagt tgcagattgg cacattgaaa gtgtctatga tgacaccttt gcgctgaaag
      901 agcttgccag aagaacagat gtcattacct atgaattcga aaacgtcagt atagaggcgt
      961 taaatgcgat tttacccatg tcatttattc ctcaagggac agacttgctt gcgattacgc
     1021 aagaccgcct gttagaaaaa tcttttttgg aaacgaataa catcgtgatt gctccttatg
     1081 caacgatcgt cagtccgacg gatatccaag atgcgattga cgggatcggc tatccttgtg
     1141 ttttgaaaac cactcgaggc ggctatgatg gcaaggggca gtatgttttg aaaagtcggg
     1201 cggatctagc tcctgccatg gaccttttaa gagaaggaac ctgtgtttta gaagcatgga
     1261 tcccgtttga aaaagagatt tcgatcatgg tggcaggaaa tggtcaagga gatttcacga
     1321 cttttccggt cgtggaaaac atccaccaca acaatattct tcatgaaacg attgctccag
     1381 ccgcgattga tcaggatgtg atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag
     1441 cagtgagcct ttcaggtgtt ttaggggtag aaatgttttt aacaaaaaca ggcgggctat
     1501 atgtgaatga attagctcct cgtcctcaca attctggaca ttattctatt gaagcttgtt
     1561 ctatgagtca gttcgatgcc catattcgag gtatttgtgg ttggccatta ggagaaaaag
     1621 tacgtttgat aagcaatgca gtaatggtca atgttttggg agaacaactg ctggaaagct
     1681 atcgcttgat tgagaataag ccagactggc aattccattt ctatggaaaa aaacaagcaa
     1741 aaaaaggtcg caagatggga catatcacta tcttgacaga agacatccag caaacgctgg
     1801 aagaaatcaa acaaacgaat atttgggatt agaggagaaa aaacatgtta gaacgttata
     1861 cacgccctga aatggggaat atctggacgg aagagaacaa ataccgtgcg tggctcgaag
     1921 tcgagatttt agcagatgaa gcttgggcag aacttggtga aatcccgcaa gaagatgtga
     1981 aaaaaattcg tgagcatgca tcatttgata tcgatcggat cttggaaatc gaaaaattaa
     2041 cacgccatga tgtcgttgcg tttacacgag cggtttctga aacattggga gaagaaagaa
     2101 aatgggtcca ttatggtcta acaagtacgg atgtcgtaga tactgcttat ggctatctca
     2161 tcaagcaagc aaatgatatt ttacgggaag acttgaaacg ttttacggaa atcattggcg
     2221 aaaaagcaaa agaacacaaa tatacagtga tgatgggtcg gacacacggt gtccatgctg
     2281 aaccaactac ttttggattg aaacttgcca catggtattc agaaatgaaa cggaacatcg
     2341 aacgatttga acatgcggca aaaggagtag aagcaggaaa aatctcagga gcagtgggaa
     2401 cgttcgcaaa tatcccgcca tttattgaag agtatgtatg taaacaattg gggatccgtc
     2461 cacaagaaat ttctactcaa gttctccctc gggatctaca tgctgaatat ttctcagcga
     2521 tggcgcttat tgctacaagt attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat
     2581 ccgaaacacg tgaagtagaa gaattttttg cgaaaggtca aaaaggttct tctgctatgc
     2641 cgcacaaaag gaatccgatc ggttcagaaa atatggctgg tctagccaga gtggtgcgcg
     2701 gacacatggt cactgcctat gaaaatgtca cattatggca tgaaagagat atttctcatt
     2761 cttcagcaga acgaatcatc ttgcctgata ccacgatttt attgaattac atgttaaatc
     2821 gttttggcaa tatcgttaaa aatctaacgg tctttcctga aaacatgaag cggaatatgt
     2881 ctgcaacatt tggtttgatt tatagccagc gcgttatgct gaaattgatc gataagggaa
     2941 tgacaagaga agaagcttat gatcttgttc agccaaaaac tgcgtacgct tgggacaacc
     3001 aagtgcagtt ccgcccgctg ttagaagaag atccaaccat cacatcggta ttatctaaag
     3061 aagaaataga cgatgccttt gattatcatc atcatttgaa aaacgttgac gtgatattca
     3121 aacgagtagg tttggaataa agaagagtga gttgcaaaat tcaattaaaa tagaatagca
     3181 aaaataacca aaaaagaagc agatccgtta gcggttctgc ttcttttagt ttattatatt
     3241 ttcttatcaa taataggaac ctgattttaa aagcggctac ctctttaccg tgcctcttta
     3301 catacaaaaa atagaggaat taagcgtaaa tggcttggct attataacta aaatgctggt
     3361 tatggagatg ttgcagcaag ttttcgagaa aagcgtattc catctcttgt ccattgaata
     3421 agatctggat acgataatga tttttgaaac gcttctgttc atgaatctga ctgattgaaa
     3481 gttcgccttt gtgttctgtc tgataaagaa aagtcctttt acggcattca gcacagcgct
     3541 tggttgctcg gcggattttt cggaatgtat aaaaactagt atctttattg cataaataaa
     3601 cgtctttgat cgacagcaaa tcattatata gacaggaacg ttcttcagct gttaattctg
     3661 ataaaaaaag ctgaccttgt tttattgcat cttccatcgg caagtgcctc ctcgaaccca
     3721 ttatccaaat ttttagttaa ttctaatata ccatcattaa gtataaatgt aaacgcttgt
     3781 tgatggtata agattttcgt ttctgataaa acacgaacat taaaagaatc taatgcctta
     3841 aatgtacata tttttgttga tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac
     3901 gaacaaaaaa cgatgtattt gcttttaatg taaataaaaa ggagagacgt acatggagaa
     3961 gaaagaactt ttgtatgaag gaaaagccaa aaaaatatat cttacttctg atgagaactt
     4021 gatttggata gaatatttag atcaagcaac tgcgttgaat ggcgcgagga aagaccagat
     4081 aagtggaaaa ggagaattga ataatcaaat cacaagtgca attttccgtt atctgactca
     4141 aagaggaata aaaaatcatt ttattgaaca attgtctaaa accgaacaat tagcaaaaaa
     4201 agtacaaatt atccctctgg aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa
     4261 acgtctaggt gttcccgaag gtaaagaatt catcaaacca atcattgaat tctactataa
     4321 ggatgaccag ttggatgatc catttatcaa tgaagagcat atctatttct tacaacttgc
     4381 aaagccggaa gaaatcagtg agctgaaagc ccaagcgttg gctattaatg aaattttgac
     4441 cgaattattt gctaaggtcg gtttggtttt agtagatttc aagctagaat ttggccggtc
     4501 gaatggacaa attttgcttg cggatgaagt aacaccggat acttgccgat tatgggatga
     4561 acgtacgaat gaacatatgg acaaagacgt ttaccgcaga gatctaggtg atttgatccc
     4621 agtctatcaa gaagtatata accgtctatc ggaactaact gaagaaaagg agcgagtatg
     4681 atgtatttcg taaaagtata tgtaacctat aaagattcag tgttagatcc tcaaggggag
     4741 gcagtaaaaa aagcagtcca tcgtatggga tatgaaacaa tcgaagatat ccgaatagga
     4801 aaatactttg agatacaact tgctgatgca gaagaaccaa tcgatacagt gattgaaacg
     4861 atttgtgaca agctgcttgc aaatgtaaat atggaaacat accgatatga aatcaaaaaa
     4921 atggaggaag tataacatga aatttgcggt catcgttttt ccaggttcaa attgtgacgc
     4981 agatatgtta tgggcagttc gcgagatcat gggcgctgat gccgaatttg tacggcatga
     5041 tgagtcctct ttagaaggat ttgatggggt tttactgccg ggaggattct cctatggtga
     5101 ctatttgaga tgcggtgcga ttgctcggtt ttctacaatc atggaagaag tgattcgttt
     5161 tgctgaagaa gggaaaccag tattcggtac atgcaatgga ttccagatat tgacagaagc
     5221 aggacttttg ccaggtgtac tgagacgaaa tgagtctttg cattttattt gcaaaactgt
     5281 accattgaaa gtcaacccca agacggcctt tacttcagag tatgaagccg acgaaataat
     5341 ctatcttcca gttgctcatg gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga
     5401 actaaaaaaa aataatcaga ttgcctttac ttataatgac aacatcaacg gcagcgtgga
     5461 aaacattgca gggattacga acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga
     5521 acgtgcagta gaagacttgc taggatcaga agatgggaaa cggttctttg cttccatttt
     5581 aaaaaacttc ggaaaggtga ccgtatagat gaaaacattg aaagaaccga cagctgaaga
     5641 gatcaagaaa aaaagaatct atgccgaatg gggattgact gatgaagaat atcgaatgat
     5701 cgaagaagat atcttaggaa gaatgcctaa ttatacggaa actggactgt tttctgttat
     5761 gtggagcgaa cattgttcct ataaaaattc taaaccggtt ctacgcaaat ttcctactag
     5821 tggacctcat gtgttgcaag gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg
     5881 actagcagtt gtcttcaaag cagaaagtca caatcatcct tcagccgtgg agccttatga
     5941 aggagctgcg acaggagttg gcggaatcat cagagatatt ttcagcatgg gggctcgtcc
     6001 aattgcgttg ctagattcat tgaggttcgg cgaattagat aatccaagaa cgaaatattt
     6061 actagaagaa gtagttgcag gtatcggcgg atacggcaat tgtatcggca taccgactgt
     6121 aggcggcgaa attgcatttg atccttgtta tgaaggaaac ccattagtca atgcaatgtg
     6181 cgtaggtttg attgaacaca aacatatcca gaaagggcaa gccgctggcg tcggtaattc
     6241 gatcatgtat gtaggtgcaa aaacaggtcg cgacggtatc cacggagcaa cttttgcctc
     6301 agaagaattt aatcaagaag aagaacagca acgttctgct gtacaagtag gcgatccttt
     6361 tatggaaaag ctgctgcttg aagcgtgttt ggaattaatc ttgcatcatt cggattctct
     6421 aataggtata caggatatgg gagcagcagg acttgtttca tctagtgcgg aaatggcttc
     6481 aaaagctggc tcaggactta tattgacatt agatgaagtc ccacaaagag aaactgggat
     6541 gacaccttat gaaatgatgt tatcggaatc acaagaacgg atgttgattt gtgttaaatc
     6601 aggagaggaa gaaaaaatcc aagaactatt tcagaagtat gatctagatg cagtcactat
     6661 cggtaaagta acagacgacg ggcaatatcg gctatatcat caaggagaag aagtagcaaa
     6721 tctgccagtc gatgcattag ctgaagatgc accagtttat cataaagaaa tgaaagaacc
     6781 agctcgaatc gtagaatttc agcagttagc tccttatcag ccagttatcg aagaacctgg
     6841 acaagtattg cttgatctgc tgcagcaacc gacgattgct tcaaaacgtt caatctacga
     6901 aacttatgat tcacaagtac aaaccaatac agttgtccgt ccaggaagcg atgctgctgt
     6961 gatgagggtt agagggacga ataaagcgtt agctatgacc acagactgca acgcgcgcta
     7021 tctgtatttg gatccaaaaa tcggaggaca aatagcagtg gcagaagctg ctagaaatat
     7081 tattgctagc ggtggtaagc ccttggcgat caccgactgc ctgaactatg gttctcctga
     7141 taaaccagaa gttttttggg aattatcgac ttcagctgac ggaattgctg ctgcgtgtga
     7201 aacgttagga actcctgtta tttcaggaaa tgtctcttta tataatgaaa cggatggtca
     7261 agcaatctat ccaacaccaa tgattggtat ggtcggattg attgaggatc ataaacatat
     7321 cactactcag gaattcaaaa aatcagggga tctaatctat atattaggta agacgtttgc
     7381 agattttgat ggcagtgaat tacaaaagat gcagcttgga agaatcgaag gtgtgatccg
     7441 aaactttgat ctatccattg aaaagagaaa ccaagagtta gtattgacag ccatccaaaa
     7501 cggattgatt gaaagtgctc atgactgttc agaaggtggc ttggcaattg cactggcaga
     7561 atcagctttc aaacatcagc tagggatatc tgtacagttt gaattgtcct ctgctcaatt
     7621 atttgcagaa actcaatcaa gatttgtgtt gacagtcgca ccggaaaata agacacgatt
     7681 cgaagaaatg atgggagatg ctgctgtttt agcaggtaag gtgacggatg aagcgataat
     7741 cgaaatttca gcaactgacg gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg
     7801 ggaggatgca atcgtatgtc ttatgaagtg aagagtttaa atgaagaatg cgggatcttc
     7861 ggtgtttggg gtcatccaga cgccgctagt gtggcatact tcgggctcca tagtcttcag
     7921 catcgaggac aagaaggtgc aggtatcgtg tccaatgatc gaggaaagct taaggggcat
     7981 cgagatttgg ggctgctttc tgaggtattc aaagatcaaa gaaaattgca gcagttaaca
     8041 ggagaagctg caatcggaca tgtacgttat gcaacagcag gcaatggaag tgtggacaat
     8101 attcagcctt ttctattcaa atttcatgat cagtcgatcg gcttagcgca taatggtaac
     8161 ttgatcaatg ctcgttccct tagaaaagag ttagaggaaa atggggcgat cttccattct
     8221 aactccgata cggagatatt gatgcatttg atccgacgaa gtgaagaaac agatttttta
     8281 aataaattaa aaagtgcttt gaatgaagtc aaaggtggct ttgcctattt attaatgaca
     8341 gaaacggcaa tgattgccgc tttggaccca aatggttttc gtccattagc aatcggacaa
     8401 atgaaaaacg gagcatacgt gattgcctcg gaaacttgtg cattagaagt tattggggca
     8461 cgttttataa gagatgtcca gcctggtgag atagtgatca tcaatgacga aggaatccag
     8521 attgattgct ttacgaagga cacgcagctt tctatttgtt cgatggaata tatctatttt
     8581 gccagaccag attcaaacat cgcagggatc aatgtgcata ctgcacggaa aaatatggga
     8641 cggaatttag cgaaagaatc gccagtaaaa gcagatatgg tcgttggcgt acctaattct
     8701 tctttgtcag ccgcaagcgg ttatgcggaa gctagtggaa tcccttatga aatcggatta
     8761 gtaaaaaatc aatacattgc ccgaacattt atccagccaa cgcctgagct aagagagcaa
     8821 ggtgttcgaa tgaaattatc tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg
     8881 gtcgatgatt caattgttcg gggtacgaca agcagacgga tcgtccatct tttgaaagaa
     8941 gcaggggcaa aagaagtcca tgtgaggatt gcttctcctc ctttgaaata tccatgtttt
     9001 tacgggattg atattcaaac gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa
     9061 atcaaagaat gtatcggtgc agattcttta ggttttttaa gtgaagaggg tctgatcgaa
     9121 tcgatcggtc tggaaaaaga tgctccgtat tcaggattgt gtatggctta tttcaacgga
     9181 gattatccga cgccgttata tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa
     9241 gtatcgttct tttaaaagca gaccaagaag ggagtatatg aattcaatgg caaatgcata
     9301 cgcaaaagct ggcgtagatg tcgaagctgg atatgaagta gtagaacgga tcaagaagca
     9361 tgtcaaaaaa acggaacgtt taggtatgat gggcgctata ggaggattcg gcggttgttt
     9421 tgatttatcg gtctttcaat taaaagagcc agtccttgtc tcggggaccg atggcgtggg
     9481 gacgaaatta gcagtggcaa tcgaagcaga caaacacgat acaatcggga tcgactgcgt
     9541 agcgatgtgt gtcaatgata tcttagcaca aggcgcagag ccgttatatt tcttagacta
     9601 cttagcaacg gggaaaaatg atccgttacg tttggaaaat gtagtaaaag gtgtagcaga
     9661 tggatgtgtt caaggaaata tggcgctgat tggcggagaa acagctgaaa tgcctggtat
     9721 gtactcaggt aatgattatg atctagccgg atttgctgta gggatagctg aaaaatctca
     9781 attattgaca ggagaaacga tcaacccagg agatgtgttg cttggaatag catctagcgg
     9841 gattcattca aatgggtact ctcttgttcg caaaattttc tttgagcagc agcagttttc
     9901 tttatcatca aaactgccag aattgaaaaa aacacttggc gaagaattgc ttgagccaac
     9961 gaaaatctat gtttcttcct tgcttccgct gatcaaagaa aagaaaataa aaggagctgc
    10021 acatatcact ggtggagggt ttgtagaaaa tatcccgcga atgttaccag aatctatggc
    10081 tgcacaaatc aaacttggct catggccgat tcttccaata ttcggctgtt tacaaaaata
    10141 tggtgaaatt cctgagatgg agatgtatga aatcttcaat atgggcatcg gtatggtcct
    10201 ggcagtcgaa gaatcgaaag taaaagaaat agtacacgaa attgagcaag ctggcgaaaa
    10261 agtttatgaa atcgggaaag tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga
    10321 agaacgatga gaatcgctgt ttttgcttct ggaaacggaa gcaactttca agcgctagcc
    10381 gattatctat cgaaaaaagg attggaatct tcgattgact ggttgttttg tgatcagcct
    10441 gaagcatatg ttttgaaacg tgcaactgcg ttgagtgtac cggcagattg tttttcacct
    10501 aaagaatttg attcaaaaaa agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa
    10561 aagatcgatt tgatcgtatt agctggatat atgcgaatca ttggaccggt attgctgaaa
    10621 aattatgata aaagaatcat caatatccat ccgtctcttt tacctgcctt ccctggtttg
    10681 catggcatac gtgatgcctt tgaagctgga gtaaaagaaa cgggtgtcac aattcattat
    10741 atcgaccaag gtgtagatac tggaccaatc atccggcagg aaaaagtgag aattgaacag
    10801 gaggacacgt tcgattcttt agaagaaaaa atccatagag tagagcatcg aatctatcca
    10861 gaagtgatta gtgaaattat tgaaaatgga ggctatctga aatgacaaga gcattgatca
    10921 gtgtatcaga taaaactggg attgtagcat tcgcccaagg tttgattgaa caaggagtag
    10981 aaatcatttc aacaggagga acaaagcatg cgttggataa agctgggatc aaaacgcttt
    11041 cgatcgaaga agtgacaggg tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc
    11101 ctaaaatcca tggtggtctt ttaggaagac gcgatctccc tagccatatg gaggcaatgg
    11161 aaacggaaaa tatccaacca atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa
    11221 cgattatgaa accagatgtc gaaatagcag aagctattga aaacattgat atcggtggtc
    11281 ctagtatgct ccgtagtgca gcaaaaaacc atgaatttgt cacctctgtt gttgatccat
    11341 ctgactatga agaagtactt tcagaattac gtcagtatgg gcatacttca ctttctttca
    11401 gacaaaaact agcggcaaaa gtctttcgtc atactgcggc atatgatgca ctgatcgctc
    11461 aatacttaac ggattgggtg gaagaaaaag aaccggaaaa attgacactt acgtatgaac
    11521 gaaaacaaac gcttcgttac ggtgaaaata gccatcaagc cgctgccttt tatcagtccg
    11581 ctcttctgga aaaatattcg atcgcagcag caaaacaact ccatgggaaa gaactttctt
    11641 ataataatat ccgagatgca gatgctgcgc tacggatcat gaaggaatat gacgagccaa
    11701 cggtagtcgc cttgaaacac atgaatcctt gtggtattgg gacagcatct acgattttag
    11761 aagcgtggga agcagcgtat gaagcagatc ctgtttcaat ctttggtggg atcatcgttt
    11821 tgaatcgaga agtagatctg ccaacagcag aacagatgca taagctgttt ctagaaatca
    11881 tcattgcccc ttcttataca caagaagctt tggaattact gcaaaaaaag aaaaacatcc
    11941 gattactgac agtagacttt gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag
    12001 tcttaggtgg acttcttgta caagatcaag atctggcaat agaaaatgaa gaagagtgga
    12061 aagtcgtgac aaaacgtaag ccgactccag cagaagcaaa agcattagtg tttgcttgga
    12121 gagctgtcaa acatgtgaaa tctaatgcga tcgtactggc aaacgaacgt cagacagtag
    12181 gaatcggagc cgggcagatg aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc
    12241 aagagaaaat ggaaggtgca gttttagcta gcgatgctta ttttccaatg gatgattcgg
    12301 ttgaatatgc agccaaacat ggtataaaag caatcattca gccaggagga agcatcaaag
    12361 accaagattc tattgatatg gcgaataaat acgatctagc aatggtcttt actgatgttc
    12421 ggcattttag acattagaga ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg
    12481 gtcgtgaaca tgcaataagt aaaaaacttt tagccagtcc gaaggtggat acggtgtatt
    12541 gtgcgccagg aaatccgggg atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc
    12601 gagatcattc agcgttaatc cattttgtta agaaagaggc tattgattgg agtatcgttg
    12661 gtcctgaagt tcctttgtta aatgggatcg tcgatgattt tatggcggaa ggattgaaaa
    12721 tcttcggtcc gacaaaagca gcagctctga tcgaaggttc gaaaagtttt gctaaacaat
    12781 taatggatac ttatcaaatc ccgacagcta aatccagaag ttttgaatgt tacgaaaaag
    12841 cagcagcgta tatcaaagaa aatggtgcgc caatcgtagt gaaagcggac ggcttagcag
    12901 ctgggaaagg tgtagtcgtg gctaccacag tgaaggaagc gctggatgcg gcagaacaaa
    12961 tgttgaacca gcatcgattt ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg
    13021 caggagaaga attctccctt ttggcgtttg tccgtaatac caaggtgtat ccgatggtca
    13081 tttcgcaaga tcataagcgg gcatttgata acgataaagg gccaaataca ggcggaatgg
    13141 gtgcttattc accagtaccg caaatttcaa aaaagatgat tgaaaaagct gtggaagaag
    13201 ttttaaaacc agcggctttg ggaatgaaag aactaggacg ctctttcacg ggtgttttat
    13261 atgcgggttt gattgctacg aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg
    13321 gtgatcctga aacgcaagtc gttctttctc ggttgaagag tgactttgcg caagtaatcg
    13381 atgatttatt agagaatcgt acagtagagt taaagtggca gcaagaagga tacaatgtag
    13441 gcgttgtggt tgcagctgcc ggttaccccg aagaatatga gacaggaatg gttctaccag
    13501 attttctgga agaagagctt tctgtttact atgctggagt ttcctcaaat aacggcaaac
    13561 tagttggagc aggaggacgt ctttatttag tagaagcata cggtgagacg atcaaagaag
    13621 cacagaaaaa aatttacacc gcgttaatgg ataaaacgac agatggaaca ttttatcgta
    13681 cagatattgg agaaaaagct gccaatagcg aactgatcgt gaagtaaatc agctgcttcc
    13741 atttataaaa aatacaacaa acataatcgg aagcgccgat tttagtgaag ccttttcata
    13801 aagagtgtgg tacacttttg ttgtgtatat aaataaggag gagttcagat gaaaaaaatt
    13861 caattaggta cttctgatca gaaagcagct gcagtgatct taggctgtat gcgtatcaac
    13921 aatgcggaag atccagtaaa agtgattgag acagcttatg aaaacggcat tgattttttt
    13981 gaccatgcag atatttatgg aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca
    14041 aaaacatcaa tcaaacgaga agatcttttt attcaaacaa aatgtggaat cgttccaggt
    14101 gtgatgtttg atttttctaa aaaacatatc attgaatctg tagaaaaaag tttgacccgt
    14161 ctgaaaatgg attatgtgga tgctttactt cttcatcgtc cagatacatt ggtagaacca
    14221 gaggaagtag ctgaagcgtt tgatgaattg gaacggtcag gaaaagttcg ttattttggc
    14281 gtcagcaatc aaaaaccaat gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta
    14341 gtagctaatc agctgcaatt tggcttgaaa catactggaa tgatcgatca agggattcat
    14401 gtcaatatga cagatgaagc aagtatcgat catgacggta gtgtacttga ttattcccga
    14461 ttgcatgata tgacgatcca agcatggtca ccataccagt atggattttt tgaaggtgta
    14521 tttatcggga acgaaaaatt ccctgaatta aaccaaacat tagaagaact agcagctaaa
    14581 tatcaaacga caccaactgg cttggcttct gcttggattt tacgccatcc agccaatatg
    14641 caagtgatcg ccggtacgat gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa
    14701 attaatatgt ctcgggaaga ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct
    14761 taaagattgt cataagcagg tcaaaagggc tataaattaa aaagacagaa taacttgaaa
    14821 gctgttttca gcttcgagat attctgtctt tttttattgc aaatggaaag agtatcattg
    14881 tacggatttc gtttattagt tatttttgtt tttaaggaaa agctgtgtcc gatattcttt
    14941 tggagaaatc ccttgttttt tcttaaataa ataactgaaa tagttagggt cattataacc
    15001 aatttcaaaa gcaatatctg aaatacgata gtctgattgc tgcaataatt tttttgctaa
    15061 ttcgattctt tgttctgtca gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat
    15121 cgtactgaaa tgagagggac ttaatgcaac ttgttgagcg acccaagtta aggacatatc
    15181 tggatcggta aaattttctt tgataaaatc caatgcatgg ttcaaaattg gctgatattt
    15241 gttccttgaa ggctgtgagg gcagagcagt taagaaagaa atgaacatat aaactgtcga
    15301 acggtaaagc gagtcgtcgg cgctgattgc taagcgttga ctggtggatg tgcaatgaac
    15361 catttcttgc gggattttct tttctttcag gcagacatgc tggagtagct tagtcaagac
    15421 aaataaacga tataatcgtg ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat
    15481 gtacttagat acttcgtttt tcgataatga agacaaatca agtgagaggt caaagtaaaa
    15541 gacagaatca actggaagtt tcgcttcttg aatcaaatcc tcatagctga taatttgttc
    15601 tttaagggaa ctggcggttt caagtatcat ctgcgtgatc tcaaaagatg caggtaattc
    15661 gctgattctt tctgcgacag gaccaatggc gacagaaaaa ttattttttt gtcccttttc
    15721 taattcatgg accaacgttt gggctacctg ataactggtc tcgatcacat tttcttcaag
    15781 agaatcaact attaaaaatt tgatgaatcg agaggaaaca gcagagaaga tcacttgttg
    15841 attctcccta aataaaaact tcaaataatc actgagatga gcgtaatctt caaagtctgt
    15901 actgacttgg tttgctgcta ataatacggt catttttttc cctgctaaag agcgattgaa
    15961 agattcagca agtttcaatg tttcactgat cgtaagtgag cctttgaaca aaccgttcaa
    16021 gaaatgattt tttttcaatt cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg
    16081 caaatctaaa gcagttactg ctttttctat tgcttgtttc aattcgctac ttttaatcgg
    16141 tttaagcaga taatcatctg cctgtacctg gattgctcct ttcatataat cgaaatcgtc
    16201 gaatccacta ataaatatga gacgagtcca aggaagtagt ttccttgctt cttttgaaaa
    16261 agtcaacccg tccatgaaag gcatttggat atcagtcaga atgatgtcgg gctgtaagtc
    16321 gataattgaa gacagtgcca tctcaccatc tccagcttcg ccgacataag tgattggata
    16381 ataagaacca aggacagtta tttgttcccg caagttattg cgaatgaggt attcgtcttc
    16441 gatgataaaa actttataca tgtttgtttc ctccaattgt tttgggagat agagggatca
    16501 tcagtgtgac agttgttcct tctcggtatt tgctgtcaat cgtgataggt ttttgatcat
    16561 ttccgtaata aagaaggaga cgcttgttta cgttgtaaag tccgtagccg atactgaaat
    16621 ctgaatcgat ccctttggct aactctttac gtatttctgc cagtttttct gcagggatgc
    16681 caatcccgtt gtctataact ttgaattgga tcatctcatc aattttgcat gtggtaatac
    16741 gaataaatcc tttgtgacgg acgtacttcg ttccatggta tacagcattt tcaatcaatg
    16801 gctgcaagat catttttaaa atagaaacag gaaaaagtgt ttctggtata tcgatcttat
    16861 agtcaagcat atggccgtat cttattttta agatgactag ataatcattg atatggctaa
    16921 tttctttttc aagaggaatc cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg
    16981 aaagtgcatg ggtcatagtc agagcttctt caaagcagtc ttgttcgatc aaagaaataa
    17041 tggcatccaa actgttataa ataaaatggg gagtaatctg agcttgcaat acacgaactt
    17101 cactttgtgc taaatggtat tgtttcaaag cgttctcccg taaagactgg tttagatctt
    17161 ccgccatttt attcatgcta gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta
    17221 aacgaaagcc aaggttccct gaagaaagtt gttctgacat ctcgatcagc tgattgatcg
    17281 gttcttggac ctttctctct aaaaagcgac gattcttttt cgcatagaaa aaaatggtac
    17341 taagaactcc aagttccact attgttaata gaatgagcga gcggacaagt gtcgtatttt
    17401 ttttgcttgc taagtcgatc tccacacgaa caaaatcttg taagatatct gacagcagtt
    17461 gagtgacaga atctacctga ttgatgatct cttcattggt atccataaaa tcttttgcca
    17521 agatattatt gatcagctcg ttttgataat cttccagtgt atcgatcgta cgtagcgata
    17581 catctaacgt actaatttct ttaagtgtat ttgtattatt tttgatctct cgaatatcat
    17641 tcctaagttc ttctaggata ttttcctcat tgaactcatc tacggttact tggccagaaa
    17701 tcaagtccca catactttcc aaaacttttt gatcaacctt caaagcaatt gcatctgctt
    17761 tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc
    17821 tcagtagcaa agggataaca gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct
    17881 gttttatgct taaatttttt gtcatgatac ttctccagtc aataatttct ggttgggatg
    17941 ttagctatat tctgctggga aaagacagtc tcggaaataa aaatatcttt aggtaatgtt
    18001 tttcctgttt gaatatttcc aaaataacga gtaacggtat tggctacata ccagccgaca
    18061 tcaggtttac actcgactac acaattgacg attcctgagc gtagcttctc gatcatatct
    18121 tcttgtccat caatagttac aatgatcaaa tcttctccag gtttgattga tgtttctttt
    18181 atcgcagcta aagcaccgtg agtcatttca tcactatggc tgtataatac atcgatttcg
    18241 gataaatcat ttgtttctaa atatcgtttc atctggtctt ttcctttcat ccgaataaaa
    18301 tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat ggtcgatggt tccatctttt
    18361 tttagggttg gtttacgatt gattgattcc aaaaatcctt cggagcgtag cttggtcggt
    18421 gtggagttct ctgaaccagc aagttctagg attctgattt cctgtttctt tgaatttgtg
    18481 aagtgattgc tgacaaataa acccgcccga tttccttctg ctttgaaact ggggccaatg
    18541 tgggtgacga aaagctcggg atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg
    18601 atccctgctc gctttgcttc ttttaataca gagtcccacc cggattcttg caaaggagta
    18661 aaaatgatca tatccacttt ataggcaatg aacgtccgta tatcttggat ttgccgttcc
    18721 tgattcatgt agccatttcg gtagagaact tggtaatctt ctttttcaag cgcagtgata
    18781 attgattcag tatgcttttt ccgccagcta ctctccgttc ctgattgcga aaaaccaacg
    18841 actagtgggt caggtgcttc ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt
    18901 acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa atcactccat ctggatttgt
    18961 tagtactatc ttatcagata aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa
    19021 tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat
    19081 cccataaaaa aatcaatgtt aacgctttct ttattagcta tactcggtgt atcaattgaa
    19141 atgggggata aaaaatgaaa aacaaattta gaaagattgt tgggactatc gctattttga
    19201 ccgcaagcat tctattaagt gcgtgtggca attcaggatc tgcagatgat agtacggggt
    19261 atgtaggaat cgctatgccg accaaatcag cagaaagatg gatcgctgat gggaacaaca
    19321 tggtctcgga attagaaaaa ctgggttata aaacagatct tcaatatgga gaagataaag
    19381 tagaaaatca agtagcacaa atagaaaata tgattacaaa aggggtagat acacttgtga
    19441 ttgcttctat cgatggttcg gctttaactg atgtgttagc taaagcaaaa gaagcagata
    19501 tcaaggtgat cgcctatgat cgtttactga tgaattcaga aaatgtcgat tattacgcga
    19561 cttttgataa ttttggagta ggtgtatcgc aagcagctta tattgaagag cacttaggat
    19621 tgaaagaagg aaaaggacca ttcacgattg aattatttgg tggctcgcct gacgacaaca
    19681 acgcactaat caattacaac ggagtgatgt ctgttttaca accatatatg gacaacggtc
    19741 agttggtcgt gccatctgga cagacgagtt tcagtcaaat cgctacgctt cgctgggatg
    19801 gttctacggc gcaagcaagg atggataatc tgttaagcgc aaattatact gaccaaacat
    19861 tggatgctgt tttgtcgcca tatgatccaa taagtttagg aatcatttcg tcattaaaag
    19921 gcgtggggta tgggagtgaa agtaaacctt tacctgtgat tactggacaa gatgcgacag
    19981 ttgctggcgt taaatcgatc attgccgggg aacaaacaca aacgatcttt aaagacacac
    20041 gtatcttagc aaaaaatacg attgaaatga tcaaagcaat cagtgatgga gaagaggttc
    20101 cggtcaatga tactgaaact tatgataatg gggtgaaaac tgtccctacg tatttagcaa
    20161 atacagtatc tgtcgataaa gataattatc aagcagagct gatcgacacc gattattata
    20221 aagaatccga tctgaaaaat taagaggaac caaacaagga actaaagaga tctaagtcag
    20281 agagaggata agaattatgg cggattgtat tttaaaaatg gcagatatca ccaaaaaatt
    20341 ttctggaatc cgagcgttag attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc
    20401 attgtgcgga gaaaacggag cgggaaaatc aacattgatg aatgtcttga gcggcttgta
    20461 tccatatggc tcctatgacg gggaaatctt ttatgatgga aagctgtgtc aatttaaaga
    20521 tttgaaagat agtgaagaaa aaggcatcgt catcattcat caagaattag cactaagtcc
    20581 ttatctttca ataaaagaga atatttttct cggaaatgaa caagcagaaa gaggcgtgat
    20641 tgattgggaa ctgacagaaa agaaaacata tgaactgctg aataaagtcg gtttgtctat
    20701 cgacccgaat cttctagtat cccagattgg cgtagggcaa cagcaactag ttgaaatagc
    20761 taaagcatta tcaaaatccg tccgtctgtt gattttggat gagcctaccg ctgcattgaa
    20821 tgaagaagaa agcgcgaatt tgctgagatt gatccgagag ttgaaagagc aaggtgtaac
    20881 ctccatcatt atttcacata agctgaatga aatcgtggat atcgcagatc gaatcaccat
    20941 tttacgggat ggacagacga ttgagacact agaaaatgaa atgatcagcg aagagcatat
    21001 tattcgcgga atggtgggca gagatttagt tgatcgttat cctgaaagaa aaccggatat
    21061 tggagaagtt tattttaaag tagaagactg gacggtctat caccctcttg atcacgagcg
    21121 aatcataaac gaccacatca acttgcattt gaaaaaagga gagattgtag gaatagctgg
    21181 actgatggga gcgggcagaa ctgaattcgc tatgagtcta tttggacatt cttatggaag
    21241 caagatatcc ggacgtatat taaaggaagg ggcagagata tcagttaaga ccgttccttc
    21301 agccattgca aacggactcg cttatgtctc ggaggaccga aaatctttag gactgaatct
    21361 gctgatggat attcgggaaa acgtgacgct gagcagtttg gataaaataa gtcaaaaagg
    21421 agtattagat aaagaaaaag aagtaactga ggcagaaaat ttccggaaaa agatgcgtat
    21481 aaaaacaaac tccatccatc aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt
    21541 cgttttatcc aagtggctga tgacacaacc cgatattctt tttttggatg agccaacccg
    21601 tgggatagat gttggggcaa aatatgagat ctacacgatt atcgaagaaa tggcagcaaa
    21661 tggcaaagct gtctgtctta tctcttctga acttcctgaa atattaggga tgtgtgatcg
    21721 aatctatacg atgaacgaag gacgtattac aggagaagtc tcgcgagaag atgcagatca
    21781 ggaagtcttg atgaaattaa tgacgaaaga agaggtgggt taagttggaa caggcaaaaa
    21841 atcttacgga taaaaaagag aaaaaactaa atttgaaaag tagagcagct gatctttttg
    21901 gtcgctacag catggtgatc attttagcgg gactacttat tgcgtttcaa ttgatgacgg
    21961 atggtatttt ctggcgtcca ttgaatataa ctaaccttgt tctgcaaaat agccatatat
    22021 tagtactagc tgcagggatg ttattagttg tgctcttagg tcatgtggat ttgtcggtag
    22081 ggtcagtgat ggcgtttgtt ggtgcaattg cgggcatgat gatggtcaat cgccaaatga
    22141 acccgtggat gacagttatc gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag
    22201 gtttttgggt agcttatgca gggataccgg cgtttatcgt gactttagca ggattgctga
    22261 tgtttcgtgg actgacacaa gttgttctag gaggccaatc tctagcacct tttccagctg
    22321 ttttccaaaa aatgtctacg ggttatttac cagacttaag cgaaggaagc agccttcact
    22381 tcttgacatt gattttagga atgatcatca cgatcgtgtt agttgttggt caatggcgaa
    22441 caagaaatcg tcggaaaaat catttatttg aagtggaatc tccaacagct ttcttggtaa
    22501 aggcggtatt tacagcagtc gttgtactag gaataactta tatctttgcc tcttatcaag
    22561 ggtatccgat tattttgatc attcttggtt tgatcgttgc agcatatggg tttttgacaa
    22621 ataaaacagt agcaggtcga cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac
    22681 tatcagggat caaaacaaag cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg
    22741 cagctttagc tggattagtt ttagctgctc gattgaatgc ggcaacacct caagcaggta
    22801 catcgatgga actggatgca atggcggcag tatattttgg tggtgcttcg acttccggcg
    22861 gcattggaac aatcatgggt gcgattgtcg gtggcttggt catgggtgta ttgaacaatg
    22921 gcatgtcgat tttaggcgtc ggggtagact ggcagcaggc aatcaaagga ttgatcttgc
    22981 ttttagccgt tgtgctagac ttgtataata agaaaaaacg aacaacgtag gagaacgccg
    23041 gttattcaga aaaaagactg tgacaagaca tttgtcacag tctttttttc gctaaaagcg
    23101 aacattcatt gtttcgtttg tatagactaa aaataatata tgttttttta ccattcgtat
    23161 aaattctctt tttgctcttg atttttttgc ttaattgagt taaattacat agggcgctaa
    23221 aaaaagcctt gattaaatta ataatgttcg tgttttgctg gatgtgaaca tcaggaggat
    23281 aatgtaatga aagtatttga ttacgaagat attcaattaa ttcctaataa atgtatcgta
    23341 aatagccgtt ctgaatgtga tacaacggtc acattaggta aacatacatt caaaatgcca
    23401 gtagtgcctg ccaacatgca gacgattatt gatgaaacaa tcgcagaatt cttagcggaa
    23461 aatggttatt tctatatcat gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa
    23521 aaaatgaaaa agcgtggatt gattacctcg atcagtgttg gcgtcaagaa agaggaatat
    23581 tcctttatag agaagttggc agaagaatcg ctaaatcctg attatatcac tatcgacatc
    23641 gcgcatggac atgctaactc agttattgat atgattcagc atattaaaaa atatttgcct
    23701 gaaacttttg tgattgccgg taatgttggt acaccggaag ccgtccgtga attggaaaat
    23761 gcgggcgcag atgcaacaaa agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc
    23821 aagacaggct ttgggactgg aggctggcaa ctggcagctc ttagatggtg tgcgaaagca
    23881 gctcgcaagc cgatcatcgc tgacggtggg atcagaacac atggcgatat tgcaaaatct
    23941 gtccgttttg gtgcgacaat ggtcatgatt ggttcattgt ttgccggtca cgaggaatct
    24001 cctggcgaaa caaaagtcga aaatggaatc gtgtataaag aatactttgg ttctgcttca
    24061 gaattccaaa aaggcgaaaa acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa
    24121 ggatctttga aagatacact agtggaaatg caacaagacc ttcaatcatc tatttcttat
    24181 gctggaggaa gagatctgga agccattcga aaagttgact atgtcatcgt gaaaaattcg
    24241 atttttaatg gagacacaat ctaaaaaata aataaaatat taggatgttg tgaaaaagtt
    24301 gtcctgcatt aagtaataag aacagccaaa caaaaatagt tctttatatg ttttgtttgg
    24361 ctggaattat tatcgcagat acaagctttt gaacatcatt attcagaata agggactgtg
    24421 ataagatttt tgtcacagtc ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa
    24481 attcggtaaa atcaaaagaa aaaatagaca atagaaaaga gaagcttatg tgggaaaaag
    24541 tagagaaaaa acaaggaccg atttatcgtc aaattatgga acagattatg agagaaatcg
    24601 aaaacggaag gatcaatcct ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt
    24661 ttggtgtgaa tcgttccacc gttgtccatg cattggaaga gcttagggag cttggggtac
    24721 taacgagcaa acgtggcagc ggatggtttg ttaatcagac tgaatggggg aaattcgctg
    24781 ttccaagaat cgattggcgg caaatgatct ctcctcgtta tgagcagacg gactggtatg
    24841 agcaaaaaat aagagaagca aagcagatcc aaaaaacatc gtttcttgat ttatacgcta
    24901 gcgagatgcc agatgagctt ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa
    24961 tcttagcgga agaaaaagaa atggatcttt taggctatac tccgttacgt aacaaaatca
    25021 aacggtattt ggaaagtaaa tttgaattgg cattttcccc caaccagctt cttgtgactg
    25081 gtggcggtca gcaagcaatc tttttgattt tacaaacgat tctttcagct ggagagggga
    25141 tagctgtaga gtcaccttct tttttttacc gattacctct tttcaaagct tcaggaacgc
    25201 gtttatttgg gataccgatg gatgaagaag ggatcgactt agaaaaactg aaagaatcga
    25261 tccataaaaa taaattgaaa gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa
    25321 aagtgatgag tcaaaaacga cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc
    25381 caattattga agacgatgta tttacggatc tttcatttct aaaccaagaa gaagtccttc
    25441 ctatacgaac gattgatccg gataatgtgt tatacgtcgg ttcgttatct agggttttag
    25501 gaaaaacaac gaaaatcggt tgggtgatag gacctgagac ttttatctcg caactagctc
    25561 aagcgcaaga aatgatggag ttttcgatga atatccttac acaaatcaca gtggctaatg
    25621 tatttaatga agcaattgat ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa
    25681 gccgactgat aagcgcttgg ggagattcac aatcgttttt caagcttcat tcaattttag
    25741 gtggttacta tgcctggatt acttgggatg ggaaaaaact agaccccgaa ctagcagaaa
    25801 aagtagtaga tatgggtctt ggaattgcac caagtctact ttttgggcaa gatataaatg
    25861 gaatcagaat caattacagt aggctggatg aaaaccaaat accgttgttt tattcaaaga
    25921 tgaaaattct agaaaagtgg ttaacagaat aatttcacaa agtaaataga ttaaagaata
    25981 taacagcttg gatttaaatt tcaagctgtt tctttttttt cggaaaaaga atacgatata
    26041 aagaaactaa gaaaaaacca ttatttgtac gtccgttcac gatgtgtaaa taaaataaat
    26101 gtttgcaaca aaactgtata acaccaacgt ttttaaatag aaacagatgg ttgtaagttt
    26161 gattagaaaa tagaaaaaat gcagatgaaa tataaaacgc tttccttatt gtgattttat
    26221 tcactaatgc tgttaatatg cggattggca taatttcctt tcatcgtgaa aatttgtgct
    26281 tttagcttgt atttctcata aatccatgat aatttattaa tgaagacggt taaatagtga
    26341 tacagtttta ttcactgttt caatcgcaga atgaagagat tttcttatag atgagagagg
    26401 aatgtgtgac aaatggcaaa caaaaaagca ccgatcgact tccaagcact gctagatgaa
    26461 gtcaatgcag atttcccagt atatcaagca cttgatcaag atggaaacgt agttaacaaa
    26521 gatttagtac ctgatttatc cgatgatgaa ctcgttgaat taatgactca tatggtatgg
    26581 tctcgtgtat tagaccaacg ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt
    26641 gcaccaactg ctggacaaga ggcaagtcag ctggctagtg cttacgcatt tgataaagaa
    26701 gatgttcttc ttccaggtta tcgggatgta cctcaactag taaaacatgg tttaccttta
    26761 tctcaagctt ttttatggtc tcgaggacat gctgcaggta attcttatcc ggaagaattg
    26821 aaagctctac caccacaaat tatcatcggt gcacaatatg tacaagcagc cggtgttgca
    26881 ttaggtttga agaaacgaaa caagaaaaat gtcgtcttca cttatactgg tgatggcggt
    26941 tcatcacaag gtgattttta cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc
    27001 gtattttata tccaaaacaa cgggtatgcg atctctacac ctcgtgaaaa acaaactgca
    27061 gcaaaaacac ttgcacaaaa agctgtcgca gctgggattc ctggtattca agtcgatgga
    27121 atggatccgc ttgcagtata tactgtatct aaaatggcac gggaatgggc gatcagtgga
    27181 aatggaccag tcctaatcga aacactgact tatcgttatg gaccacatac actatctggt
    27241 gacgatccaa cccgctaccg tacaaaagac acagatgacg attggcagaa aaaagatcca
    27301 ttgatccgct tccgtaaata tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa
    27361 caagtcatcg aagcaactaa agaagaaatc aaagcagcta ttgctgaagc cgacaaagta
    27421 ccaaaacaaa aggtttcaga tttcttgaaa aacatgttcg aagtatctcc acaatcaatc
    27481 aaagaacaaa ttgcaattta tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa
    27541 tgatccaagc aatcacagat gccttagcac ttgaactaga aaatgacgag aatgtcgttg
    27601 tttttggtga agatgttggg aaaaacggag gagttttccg ggcaactgaa ggattgcagg
    27661 aaaagttcgg agaagaccga gtattcgata ctcctttagc tgaatctgga atcgcaggat
    27721 tgagtttcgg tcttgcgtta gaaggatttc gtccagttcc tgaaatccaa ttctttggtt
    27781 ttattttcga agcaatggac gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga
    27841 gcggcactag aaatctaccc atcacaattc gttcgccatt tggtggcggt gttcatacac
    27901 cagaattgca ttcggataat ttagaaggtt tgatcgctca gtctcctggt attcgagtag
    27961 tgattccttc caacccttat gatgcaaaag gactattgat ttcttctatt agaagtaacg
    28021 atccggttgt tttcctagaa cacatgaaac tatatcgctc attccgagaa gaagtgccgg
    28081 atgaagctta cgaagttcct ttggataaag cagcagttac tcgtgaggga acagatgtat
    28141 ctattatcac atacggagca atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa
    28201 aagaaaatat atctgttgaa atcattgatc ttcgtacagt tgcgccatta gatgtagaaa
    28261 cgatcattca atccgtggaa aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac
    28321 aagcgggcgt tgccgcgcaa gtagtttctg agatctcaga acgcgctatc ctttcattag
    28381 aagccccaat cggacgagtt tctgcacctg atacagtctt cccatttgga caagcagaaa
    28441 atgtctggtt gccaaatgca aaagatatcg aagacaaagt aaaagaaatc gcggaatttt
    28501 aagaaaagaa gggacgaaac aaagtcatgg cttatcaatt taaatt
//
LOCUS       DACEPW010000015        28437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEPW010000015 DACEPW010000000
VERSION     DACEPW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.015"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(115..918)
                     /locus_tag="IW819_000511"
     CDS             complement(115..918)
                     /locus_tag="IW819_000511"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1243641.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(938..1780)
                     /gene="kduI"
                     /locus_tag="IW819_000512"
     CDS             complement(938..1780)
                     /gene="kduI"
                     /locus_tag="IW819_000512"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ1243642.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(1796..2803)
                     /locus_tag="IW819_000513"
     CDS             complement(1796..2803)
                     /locus_tag="IW819_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ1243643.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(2810..3454)
                     /locus_tag="IW819_000514"
     CDS             complement(2810..3454)
                     /locus_tag="IW819_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1243644.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(3554..4312)
                     /locus_tag="IW819_000515"
     CDS             complement(3554..4312)
                     /locus_tag="IW819_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ1243645.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(4439..5590)
                     /gene="fucO"
                     /locus_tag="IW819_000516"
     CDS             complement(4439..5590)
                     /gene="fucO"
                     /locus_tag="IW819_000516"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ1243646.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(5672..5983)
                     /gene="rhaM"
                     /locus_tag="IW819_000517"
     CDS             complement(5672..5983)
                     /gene="rhaM"
                     /locus_tag="IW819_000517"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ1243647.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(5985..6830)
                     /gene="rhaD"
                     /locus_tag="IW819_000518"
     CDS             complement(5985..6830)
                     /gene="rhaD"
                     /locus_tag="IW819_000518"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ1243648.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(6844..8100)
                     /gene="rhaA"
                     /locus_tag="IW819_000519"
     CDS             complement(6844..8100)
                     /gene="rhaA"
                     /locus_tag="IW819_000519"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ1243649.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(8097..9557)
                     /gene="rhaB"
                     /locus_tag="IW819_000520"
     CDS             complement(8097..9557)
                     /gene="rhaB"
                     /locus_tag="IW819_000520"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ1243650.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            9710..10579
                     /locus_tag="IW819_000521"
     CDS             9710..10579
                     /locus_tag="IW819_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1243651.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(10705..11604)
                     /locus_tag="IW819_000522"
     CDS             complement(10705..11604)
                     /locus_tag="IW819_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1243652.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(11769..12593)
                     /locus_tag="IW819_000523"
     CDS             complement(11769..12593)
                     /locus_tag="IW819_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1243653.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLREIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(12604..13899)
                     /locus_tag="IW819_000524"
     CDS             complement(12604..13899)
                     /locus_tag="IW819_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ1243654.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMVHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(13915..14394)
                     /locus_tag="IW819_000525"
     CDS             complement(13915..14394)
                     /locus_tag="IW819_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1243655.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(14400..15428)
                     /locus_tag="IW819_000526"
     CDS             complement(14400..15428)
                     /locus_tag="IW819_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ1243656.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(15445..16551)
                     /locus_tag="IW819_000527"
     CDS             complement(15445..16551)
                     /locus_tag="IW819_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1243657.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(16780..19377)
                     /gene="adhE"
                     /locus_tag="IW819_000528"
                     /gene_synonym="adhC"
     CDS             complement(16780..19377)
                     /gene="adhE"
                     /locus_tag="IW819_000528"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1243658.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(19682..20062)
                     /gene="yajC"
                     /locus_tag="IW819_000529"
     CDS             complement(19682..20062)
                     /gene="yajC"
                     /locus_tag="IW819_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1243659.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(20159..21304)
                     /gene="tgt"
                     /locus_tag="IW819_000530"
     CDS             complement(20159..21304)
                     /gene="tgt"
                     /locus_tag="IW819_000530"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1243660.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            21522..22220
                     /locus_tag="IW819_000531"
     CDS             21522..22220
                     /locus_tag="IW819_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1243661.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(22388..25027)
                     /locus_tag="IW819_000532"
     CDS             complement(22388..25027)
                     /locus_tag="IW819_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1243662.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(25646..26677)
                     /gene="queA"
                     /locus_tag="IW819_000533"
     CDS             complement(25646..26677)
                     /gene="queA"
                     /locus_tag="IW819_000533"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1243663.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            26870..28045
                     /locus_tag="IW819_000534"
     CDS             26870..28045
                     /locus_tag="IW819_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1243664.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            28049..>28437
                     /locus_tag="IW819_000535"
     CDS             28049..>28437
                     /locus_tag="IW819_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1243665.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELP"
BASE COUNT         7910 a         6304 c         5051 g         9172 t
ORIGIN      
        1 gcttctgtcc cagcctcttt tttcgaataa atgatgttta atcagctgtc cttcggtatt
       61 cgttcaatcc gctgatttca caacctctcc actaaacagt gtactaaagc tcttttaagg
      121 ttgttttccg atataggcaa gtatgcctcc gtccacatac aagacatggc cgttaacgaa
      181 atcagatgcg ctagaagcca agaagacaga tggtccagcc aaatcgattg gctcgcccca
      241 gcgtgctgct ggcgtacgtc caacgataaa ttgatcgaat ggatgacgtt caccgttttc
      301 ttgtgtttcg cgtaatggag cagtttgcgg tgtagcaata taacctggtc caattccatt
      361 acattggata ttgtattgac cgtattcaga agcgatgttt ttcgtcagca tttttaatcc
      421 gccttttgcc gcagcataag cactgaccgt ttcgcgtcct aattcactca tcatggagca
      481 gatattgatg attttaccgc cgcctttttc gatcatatcc ggaataactg cttttgaaac
      541 gatgaatggg gcatttaaat ccacgtcgat tacttggcgg aagtctgccg catccatttc
      601 gatcatcggt gtccgtttga tgatccctgc attgttgact agaatatcaa ctgaacctac
      661 atcttctttg atctgttgaa ccatcgcttg aaccgcttct tcatcggtca catcgcagac
      721 ataacctttt gcatcgatgc ctgcttcttt ataatgcgct ataccttcat caactgattc
      781 ttgattcaag ttattaaata cgatttttgc tcctgctgta gctaaagagc gtgcaatctc
      841 aaatccaata ccatagacag caccggtgac taatgctact tttccatcta aacgaaacat
      901 atccatacta aattccatca ttttctctcc cttatgctta ttttaattga tccatttgca
      961 ccatatccat gtctgtatag gtgatatttt ctccgcacat tgcccagatg aatgagtaat
     1021 tgcttgtacc tacacctgaa tggatcgacc agcttggcga aataatggct tgttcattgt
     1081 ctacaactaa atgttttgtt tcatctggtt tgcccatcat gtggaacaca cgtgtatctt
     1141 cattggcata atcaaagtag acataagctt ccatacgacg ttcatgtgta tgacaaggca
     1201 tcgtattcca agcgcttcct ggttctaaaa tcgtataacc catttgtaat tgacagcttt
     1261 cgcatacatt tggatggatg tattggtaga ttttacgttc attcagtgtc aggccttcac
     1321 cagtctccat tggttttact tggtcgatac tgatttttac atttggatat ttatgatgag
     1381 ctggaacaga actaatatag aatttagctg ggttttcagc atcttgagaa gaaaatacaa
     1441 catgtcgtgt ttcttttccg atatagtatc cgtcttgttt tttcatttct tctttttgcc
     1501 catcgatctc gatgaatcct ggtccaccga tattgatcac acctagttca cgacgctcta
     1561 agaagtaatc aacacctagt tctttgtcta atgtgatttc taattctttt tcagtaggtg
     1621 tcactccacc aaagatcatt cgatcgttgt gcgtataagt caaactgatt ttccctggtt
     1681 caaatacctt ttctactaaa aattcttttc tcaattgttc cgttgaataa tgacggatat
     1741 cttctggact gtgtgtataa cgtgtttcca tttcttgcat tttcttctac tccttctatc
     1801 gaatgatttt tccagagccg catgctaaaa attgtgcgac ttcttcttga ctaaattggt
     1861 tgcagtcccc atggatcgta tgcttcaagg cagaagctgc cgtagcaaaa tcaatgattt
     1921 cttgcggaga catattggat aaaatcccat ggagcacgcc acctgcaaaa gcatctccgc
     1981 cacctactcg atccacgatt ggggtgattc tatggacttg ggattccaca tattcatggt
     2041 tcatccacaa tgtccccttc aattcatttt cacttgctga atgaacggta cgcttggtcg
     2101 aataaaacac ctgaatattc gggaactttt cctgcatttt ttgataataa taaattagag
     2161 gttgttcttc tgcttctggc gctttctcga tacctaacaa ataaagagca tccatttttc
     2221 cagctgagca gatatctact aaaggtaaaa gctgatggat tacttcgctg gcttcttgct
     2281 gtgtccataa ttttccacga tagttgatat cgaagctgat caggcagcca gctttttttg
     2341 cagcttctat cacctgtttc gtcatttttt gccatttttt agacaaagct ggggtgattc
     2401 ctgaaacatg aaatatatct attccttgga ataactcctc tagcgaccat tccaaatgat
     2461 ccatttcagc aaaacttgat ccggcacgat catagatcac tgaggcggcg cgttcgccac
     2521 taccagtttc catataataa gtacctagtc ttgatccacc agataatacc tgtttcgtat
     2581 ctactccata acattgcaag tgctttttga ctgcttttcc caatgcgtta tcaggtactt
     2641 ttgtagcaaa aatcgcttca tgtccgtaat tagctaaaga aattgctacg tttgcttccc
     2701 ctccgccata atgggcagcg aatcgttcac ttgaatcgat acgggttccc tgctctgtgg
     2761 acaatcgcaa catgatttct ccgagtgtca ctacttttcc cataagcagc tacctcttga
     2821 tttctttcat ttttgctgca tactgttgcg cgacttccgt tactttgtca aaatctcctg
     2881 tagctgcggg tgctaaaagg ttcccaccta ctccgactgt tacgacacca gcatcgaacc
     2941 actctttcat attgtccaga ctcacaccgc cagttggcat gatattgact tgaggcatcg
     3001 gcgccttgaa ggcagaaatg atacttggtc cataagcgct tccaggaaat agtttgacga
     3061 tatctacccc gcttttcagc gctgttttga tctcggtaat agtcatacat cctggtaagt
     3121 aaggaatttg atacagattg cacatctctg ccgtttcttg atcaaaggaa gggctaacaa
     3181 tatactctgc gccagctaag atagccaagc gagctgtgac agcgtctaat actgtgcctg
     3241 cgccaatcac tatttctggt tggtctttgt agaatgacaa aagttctttg attacttgat
     3301 ctgcttgagg gactgtaaaa gtcaactcaa taccagttag accacctttg atgattgcgt
     3361 ggcaagcatt caaagcttct tctttgcttt ttccgcgtac caccgcaatc actccggcgt
     3421 ttttcaatcg agataaaata tcaatttttt tcatcttctc cacctatttc ctatttagaa
     3481 attttatttt ataacttgga aatctatacc catattatac ggcagaaaag aaaaattgca
     3541 aacgcttttt tcattatttt tgaattcttt gctcgatttc gctttttgtt tgcttgaggg
     3601 cactgattat ttgttctttt gtctcatctg ttaaacgata tttcggcata cttacactaa
     3661 atgcaccaat gatttgttcg tttttcacga ttgaggctcc tatacaaaaa atatcacgtt
     3721 cgatttcttc atcgtcaaat gcgatgtggg tttcttttac cgttttgatt tcctttttca
     3781 accgtaatgc attggtgatc gtatattctg tgtaagggat caacggatgc ttttcaagat
     3841 aatcagtgta ctgctcttca ctgaattctg ctaatacggc tttgcccatc gcagaattgt
     3901 ataatggtcg ggtgattccg atttttgaag acatgcggat cgtttgatgt tttggatcta
     3961 gtttgttcac gtacaagatc tcattgttgt ccaatacccc taagtggata gtctcatcga
     4021 tcttttgctg cagctgttct aaaaacggta aagtcaactc taccaaatcg atttgttcaa
     4081 tgtttttatt cgcgtagcga ataagctttg cacctaaacg atagtttttc tctgaatttt
     4141 tatttacgta gccgatcatc aacaaagtat ctagtatttt caagactgta gaagaggtca
     4201 ttcccacacc ctttgcgatc gtctgcaaag agacatcttg gttttccgct aaatagtcta
     4261 gtattttaga ggctttgatc aatacagttc cataaggctt tttattttcc atataatatt
     4321 ctcctatttc tttataagaa aaattatagc ggaaatacca tcaatttaaa agagatatcc
     4381 agatataaac taccatttct ctttacaggc aatttaatgt tgaagatccg cttcgttttt
     4441 acatcaaact ttgatataaa gcaatgattt cttctactgt cgtttctctt ggatttcctg
     4501 gtgtgcatac atctcgcaaa gcatcttctg caattgctgg aatatctgct tcttttacac
     4561 ctaattcaga aatggtttct gggattccaa ccgctttgct caaccgatcg atctcaccac
     4621 aagccgcttc tcttacatct tctaaagaca tttcagctgc tcctttaatc cctagaacaa
     4681 gagcgatctc acggtacttt tctccagtat attctttgtt atatttcatg acagttggca
     4741 aaagtgctgc acacgctacg ccatgaggaa tgttgtacca tgcacttaac gggtgagcca
     4801 tcccgtgtac cagtcctaaa ccgacatttg agaagcccat tcccgcaata tactgtccta
     4861 atgccatttt ttctcgccca ttttgatcgc catctacaga agcttccaaa gaatggccga
     4921 tgatttcgat tgcttttaaa tgcagcatat cagtcatttc ccaagcgcct tttgtgataa
     4981 acccttcaat agcatgcgtc atagcatcca tacctgttga tgcagccaat tttttcggca
     5041 ttcccatcat catatcgctg tcgatgaacg cgacgattgg gatatcatga ggatcaacac
     5101 aaacaaattt tcggtgattt acttcatctg tgatgacgta gttgatcgtt acttctgctg
     5161 ctgttccaga agtcgttggt atagctaata taggtaaaca tggatttttc gtatcagcaa
     5221 caccttctaa actaatgacg tctgaaaact ctggattcgt caaaataatc ccaatggctt
     5281 ttgccgtatc gattggcgaa cctccaccga ttgcgatcaa acagtctgcc tctgcctctt
     5341 taacaaaagc cactccatct tttacatttt ggatcgttgg attaggtacg atcccgtcgt
     5401 ataaagcata agcgattgct gctttatcta gaagttctgt tacttttgtt gctataccgt
     5461 gctcgatcaa tcctttgtca gtaatgacag ctgctttgcc gaatcctcgc tgctgaaatt
     5521 cagggacgat cgactcaatc gctccttttc caaaatacga tgtttcattt aaaatcattc
     5581 gattactcat tttctattcc tcctaatttg tattattttt cacaataaaa acgaaagaac
     5641 cccaagcaac cggttgccca gttgcttgtt gctacagttc aaagactggc atcaattccc
     5701 aactgactgg agaggcatct ggatttgtct ccatgatatc ttccatgtac ttccaccatt
     5761 tttgattgat agatgtattt gccatttttt cccataactt ttcatcttcg atcactaagt
     5821 aaccaaataa caaacttgta ctcggatcaa gatagatcga atagtcgatg accccgtgtt
     5881 cacgaagtgc tgtacgcatt tctggccaaa gttcatcatg tctttttttg tattcttctt
     5941 gtttttctgg gtaaactttc atgcaaaaag cttttttcac catgtcagac acctgctttc
     6001 gtatgaactt cctccaaata acctgctttt ggagtgacac caaacgcatc tgctaaatgc
     6061 cacaggtttt cgtccgtgat tgtttgcaag attggtcctt gcgctttgac gtacgtatag
     6121 acttctgctg ccttttctgc tgtctcgatc aaaccaaaga cttcatccat gtcttttcct
     6181 gacccgtaga ttccatgttg tggccataaa accaatcgag tttctttcat tttttcagct
     6241 gttgcttccc caatctcatt tgttcccgga accatccacg gaatgatgga aattccttct
     6301 ggaaaaacaa ctaagcattc cgtacacatt tgccatagcg tacgactaaa ggaacgttcg
     6361 tctaattcat gcgtaaaact catcgctaat agatgactag catggcaatg cataacgatc
     6421 cgattatctg ggtctacttc taaacgggcg atatgactca tgaaatgact tggcaactcg
     6481 cttgtaggaa ggcattcgtt ttccagcccc cacaaaagtt ccagtgtttt gccatcttca
     6541 gcaactcgaa cgattccaag attctcggca ggttcgctcg ctacattttt aaaatatctt
     6601 cctgaaccag ttacaataaa atagcatcct gctaaacggc tggcatcaaa aatcatcgag
     6661 atgcggcgga tcactttttc aggatccaag aacggtgtga tctcttcttc ttttagtaaa
     6721 taagaaatat tcccgccatt tctttcatcc catcccaagc gataaagatt cgacgtcgtt
     6781 tcgatcattt ctttcacata aggcgcttgt aaaatatcta tttttttcaa tatagccaca
     6841 ctcctatttt cttaattcca acacgtcttt ttgatattcc tggacttctt ccagccaatc
     6901 caaaccaact ggaacttgat ttttgtaaca gaaataattc catacatcgg catacgggaa
     6961 atctttcaat tcttccgtat aagccattct tgaagtaaag tcaaaagcca gctccatttc
     7021 tttcaatcgc tcaactggtt ccagcatagc tttcagcagt gctttttgtg tatttctcgt
     7081 accgatgacc caagcagcga tacggttgat cgttgcatca aagaaatcca gaccgatatt
     7141 tgttttttcc aacaattcgt tacggactaa ttctttagcg atttcctgca attcatcatc
     7201 catcaaaacc acatggtctg aatcccaacg cactggacgg gaaacatgca acatgattcc
     7261 tttgctgaat aaagctaatg aagataattt attggaaatc acttctgttg gatgaaaatg
     7321 tccagcatct agacaaacaa gtttgtcacg agtcaacccg taacccatat aaaattcatg
     7381 ggaaccgact gtataagctt cagcgcctaa accaaatagt ttgctttcta ctgcatcttg
     7441 tacataccgt tcatctaact cttctgtaaa aatttcatcg agtgattcca ttaaacgttc
     7501 ccgtggggta aaacgatcaa tcggattatc tttcatccca tcaggtaccc aaaagttatt
     7561 gatacttgtt tgcccagttt ctttcccaaa gtactccgcg attttgcggc ttcttttccc
     7621 gtgttcgatc cagtattcac gaacttcttt atctggatga gccaatgtaa agccgtcttt
     7681 catcattgga tgcgaaaaga aagtcggatt gaagtcaaga ccgatttctt ctttttttgc
     7741 ccactctacc catttttcaa aatgtttcgg ctcaatttca tttagatcga cagattcttc
     7801 tgtatccaaa tagatagcat gaaggttcaa tttgtgtttg cccggtatca acgaataagc
     7861 cttctccaga tcttgtctaa gctcttcagg tgttcgtgcg gctcctggaa aattccctgt
     7921 cgccatgatt ccaccggtca attcgccatc cggtgtcaag aaacctttga catcatctcc
     7981 ttgccagcaa tgcatagaaa ttttgatgtc ctgtatcttt tctagtacag catctgtatc
     8041 tacaccgata gttgcatatt tttcttttgc ttgttcgtaa tttctttcaa tcgtgttcat
     8101 acgattactc ctttgatttt attcacgtac cattccatcg tgttttgctt attttcatct
     8161 gggtaaaaag tttcacatgg aaacgactgt ttgattacat ttcttgcttg ttctatattc
     8221 gaaaactgac ctgtactgag catttgcatg gcaacattcc cgattgctgt ggcttcacta
     8281 ggtcctgctt ctacaatgat tccggcataa tctgctgtca attgattcag tagacgatta
     8341 ttcgatccgc cccctacgat gtgcagcttt tccaatttcg ttccagttaa gtgttctact
     8401 ttgatcagct cttgtgcgta acaaagagca agattatcat acacacaacg agcgatagct
     8461 cccgatgaga cagggactgg ctgctctgtt tcaaaacagt attgctgaat ctcttctatc
     8521 atatttgggg gattcaaaaa tcgagggtca ttgatttcca tcacttgctg cagcggtttt
     8581 tcttttgcag caagctctgc caattccgca taactgtact cgtactcttg cattcgggca
     8641 acttcctgga tcaaccacat ccccatgatg tttttcaaaa aacgcaccgt gttatgggca
     8701 ccccattcat tcgtataatt ttcagaaaaa gcattgcgag aaacattggc gatctttgtt
     8761 tcaatcccta aaagcgacca tgtcccactg ctgatataac cccaacgttc tccttgacct
     8821 ggcgttccta atacagctga cgccgtatca tgacttgcta ctgtgataaa ggtcacatct
     8881 ggcaaatcat attttgaaaa ttgttctttt tgcagatctc ctagtatcgt tcccggctct
     8941 gtcaatggtg gaaataattc cttcgagaca ccgatttttt caagaagttc tatttcccat
     9001 tcttttgtcc ctgcattcag cagctgcata gtagatgcat tagttttctc cgtcaccatg
     9061 ttaccagtaa agacataccc taaataatca ggtatcagta acaatttatg tgcttttgcc
     9121 aaattttctt tttcttcagc aaacaattgg aacaaggtat tgaaaggctg gatctgaatc
     9181 cctgtctgct catacaactt atctagagaa tagatttcag aaaactgttc tactgctcca
     9241 ttggtacgct tgtcccgata agcaatcgga tcatttatcc gatccccttg ttcatcaatc
     9301 agacagtaat cgactgccca tgtatcgatc cccgcaaaaa ggcgatcaat ccctgtctgc
     9361 tttaattttt caaatccaat caaaatctct ttgataagat aatcgatctt ccagcgatcg
     9421 tgaccgtctt tgttccgaaa gccgtttttg aaccggtgta cttcttccaa gatcaaagaa
     9481 ccttttgcat ccctcgtact tttcattagt cgtccgcttg atgcaccgat atcgatggca
     9541 acaaatgttg gattcatttc tccacctctt ttactttatt gttgattagc actttattaa
     9601 taaagcgtta tcatttatta acaatgctat cataatacag aatcttatat tttgcttcac
     9661 actattttgg tatataattg cattatcttg acaagttggg agtgtttaca tggattactg
     9721 ggaaaaagcc aaccactcat ggtcggaaga ctcaaaaaga gtgatcttga cgcctacaca
     9781 aagaagcagg aatttatttt tttatatcca agaaatcggt tacttcaaag caccaatccc
     9841 ttactatact gaacgagcac atttaccttc ttacttgata aagtttactt tgtctggcaa
     9901 aggttcgctt acatataatg ataatcagca acagctaaaa gctggagact tattttttat
     9961 tgactgccaa gaatatcagc gttatcaaac gatcagcgaa gaaccatggg aaatggattg
    10021 gatccatttg aatggtcaga acgtccaatc attttatcag gagttcacaa aagacggcca
    10081 taacaccttc cattcaacag gagatccatc agaaaacaag atccaccatt tgatcacaga
    10141 attgatccac atgcaacaag gcacgaatgc ccgtaccgat ttccagtcct ctgtagctat
    10201 ccacgagttg ctgaatgaac tgattttgca aaagtatcag ctagatttcg aagaagagga
    10261 tattcccgcc tacgtttaca aactgaagca atacttagac gaacatttca aagaaacgat
    10321 tacacttgat caattagaac aacaattctt attgaataaa tatcagctga gcaaagattt
    10381 ttccaaattt atcggttctc caccaatcga ttatttgatc agcaaaaaac tttcttacgc
    10441 taaagacctt cttcggtata cagaatttac ggttcgaatg atttccgaag aaatagggat
    10501 tgagaatttt gcttacttta gccgcctgtt caaagcaaaa acaggactat cgccgagaga
    10561 ctttcgtaaa ttcagctgat tcatataata tgtacgtcat tttttcaaaa tattttgaaa
    10621 tatatagata aaaataaaat aaccgcagtt tctgcttcta tgtaaacaac agcagctact
    10681 gcggttattt tttagtgatc attctcattg ttcatcttca aaatgttcat aatgaaccaa
    10741 ctgtttctgt gtatcacgtt tcaaaaattc tttaggcgtt atgccgaact ctctcttgaa
    10801 tgctttcaga aaatgcggat aagaagaaaa accactttta ctatatacag cactagagct
    10861 gcggtattct cgcaacaagt gcttggcata gatcagcttt ttcttcatta catactgatg
    10921 aaacgtaaat ccagtgtact ttttaaattc tcgtgtgata tggtatttgc tgacaaaaaa
    10981 ctgcttttcc atttcctcta aagaaagcgg atgagataaa ttttttgaga tatattgtaa
    11041 catttgttcc acacgctcat taggaactac agcattctgt tcttgcgcac tctcttcttt
    11101 ttgtacaact tgattgagaa aaatcaagta ttctaccaga gcttgttcat attttatgtc
    11161 ttcaccgtaa atttttttat caggaggatg gtcgataaat gataattttt gcttcaatac
    11221 ttcctgttct atttgcaata cacgactcct cttctctccc attggttgaa aacaagccga
    11281 tagattagac catggcgtgg aacgactttc tagaaaagaa ggggtgacaa agatatagac
    11341 acgctcgaat acatctgaag attggcggac gatacgatgc aaatcattgg aatggatgag
    11401 cagcacactt cctgctttga tcgtaaattg tcgtccgttc aaataaaaag tcgcttctcc
    11461 ctttaatgta gcatggatct catagaaatc atgatgatga tagatcgttt tattttggct
    11521 gacgacatct ttgacatgat agatttcata ctgatcagaa accatcgtat tgacttgttc
    11581 accgctgatt ttcttttgga gcatgtgccg cacctccttt tgttcactta tgcacaattt
    11641 tattatacca cgttttttgt aatcaaaaag agctgctttt atatactctt ataaaaaaga
    11701 agagacgtgg catcttctag ccccgccccc tccattctaa atattagtta accaacgtct
    11761 tctttacatt attcaagaag acttcttcca taacgaatcc catcatcttt tgtttcttca
    11821 agaaccacat acaggtacgg acgattcttc atgatgattt ctttgatttt tgtgtactcg
    11881 atcacaccgt ctcccaaatt taccggcacg attttttcct gttcgacacg aaaatctttc
    11941 aaatgaaccg cacatatctt ttcaccaaaa cgttcaaaag cttcttctac caattggact
    12001 tgttcttgat aatcactact tgtgatcaaa ttggtcggat ctaaaatgat tcctagatag
    12061 tccgaatcta ctgcttggat cagctgctct acccttgcta aagaatacac cggatgattc
    12121 aaacctggtt ctatccccac catcatttgg tatttttctc cagcttttac caagcgacgg
    12181 ataacggaca cagcttcagc aaatgcttca tctgtgaaat tttcttccgt atattggatt
    12241 tcccgcaaaa cacatccagt ttccgaagca accatcgacg caccaaagga agaagcatag
    12301 cgaagatact gctcgaactt tgtcagcaac tcttgtcgta tcgttaaatc tggatgaatc
    12361 atattgatat agcaacttaa aattcctacg gtcacttctt gcttttctaa aactcgtttg
    12421 acataatttc ccatcccaga attgatctca tttgcgccag aaggtatttc aggaaacgaa
    12481 agacctaagg cgagctgcag tgtatgaatc ccttgttctt ttgttttttc tgcaagatct
    12541 tctatcgttt ggacttcgga aagatcatgt cctctaattg taatgttcaa cataactatt
    12601 ctcctaactc agcatattgg gtagccacat gacgatttgc gggaagtaag taatcaaaaa
    12661 caagacgact aagataaccc cataaaaagg aagaagcggt ttcagcattg cctctgcttt
    12721 tacgcctcct acacttgcac cgacatatag tcctgtacct actggcggcg tgatggagcc
    12781 tacacacaga ttcatgattg aaaacaaccc gtaatgtaca ggatcaaccc ctacacttgt
    12841 gacgattggt aaaaatattg gtgtaaaaat caagatagca ggcgccacat ccataaacat
    12901 acccaccagt aataacacaa gattgactaa taacagaata ataaatttat tatccgttac
    12961 gcccataatc aacgaactaa ttgcctgagg aataccggta aacgccatgg cgaaagacat
    13021 catggaagaa gtagccaata agaacatgat cgtccctgac attgctactg attgaaacaa
    13081 catattaggt atatctttta gttttaccgt tttatagtaa aacatagcaa tcaacaggga
    13141 gtaaatgaca gctatcgctg aagcttctac tgctgtaaat atccccgtca agataccacc
    13201 gataatgatg acaatcaata gaaggctagg aatcgcctct tttattattt ttccagcatg
    13261 aaccatctgt cctttttcga caacagggta tttattcttt ttagcaacga tgaatgctac
    13321 tagcatgatt cctagcgccc acaaagcgcc tacgagataa cctcccataa acaaagcaga
    13381 gatcgaagca ccaccagcaa caagtgaata catgataaaa gcagtacttg gaggaatgac
    13441 catacctgtc ggagctgacg cgatattgac cgctgtcgca aacttccggt catatccttc
    13501 ttccacttgc tgagggatca acactccgcc aatcgcggta gaagcggcaa tggccgagct
    13561 agagatggaa ccaaataaag cattccctaa cacattcgta tgagccaaag cccccggcac
    13621 gcgcccgacg aacaacatcg caaaatccac tagtttctta gctatccctc cattattcat
    13681 gataatgccc gagagaacga aaaacggaat agcgatcagc gaaaaactat tgatacttcc
    13741 caccattttt tgagctgaac taaaagcaga aacatcaaac ggtataacta acaataaagt
    13801 catcatcgat gagaatgcaa tacttatagc aatcggcatc cctaatacaa gcaacacaac
    13861 aaatacaaaa aataacacta atccggcttc tattgccatt ttattcctcc atttttatat
    13921 tttttcttga attgctggct tgttgcttaa tgtatcgatt agattgatca ggctatagat
    13981 aatgatgagt acccccgata caggtactga cagataaaca tatcccatag gaatacttaa
    14041 agaaggcgag atctgcccca ttgtcaatgt gacagcattc catccgccat acaccatgat
    14101 catcgcagca aataccagaa ataaactttg aacaacaact tctaaaattt tacttttttt
    14161 gccagttaat ttttcactta aaaccgttac ggctaaatgt gctttttgtc ccacaacata
    14221 cgcggaagac aacatggcaa accagacaag accgaatcga acaagctctt ccgtgaccgt
    14281 atttggattt cctaaaaccg ttctggaaaa tacttgccat aaaacaacag cgaccatccc
    14341 aacaagcaaa acagctccca ggatctctaa taccttgttt aaaatcattt tcatcacagc
    14401 tacgctcctt gctcttggat cttttgataa aggtctttgt aggtaggatc gtttttgaat
    14461 atatcatgaa gtggggcaac tgcctctcga aacggtgtat tatctacatc attgaattgg
    14521 actccatgtt cttcttgtgc taaagctttt tcttcttcaa ttgcttttgc aaaagcttca
    14581 atttcaaaaa ccgttgattc tttcgctgct tcttcaatgg ctttttcctg ctcatcagtt
    14641 agctgttctt tgatggcatc attcatgatg atgatatctg gcacacgtgt atgctcatca
    14701 taggaatagt atttagccac accaccatgt ccagctgtat acaagacaaa ttcattgttt
    14761 tctgatccgt tgatcaaatt ggattgaaga gacgtgtaca cttcatcact tcccataggt
    14821 acgggcgaac cgcctaaaag ctctaccatt ttgatagcgg tttcgctttg catcacacgg
    14881 atcttctttc ccttcaaatc ttccggcgta tggatcggac cgtctaccat ataaaagctg
    14941 cgctgaccag aatcataata ggtcaatccg gtaaatccta aatttttggt cgattggtag
    15001 attggctcca ttatttcttc attatccatc accttaaaga aatgttcttc atcattgaat
    15061 agataaggaa ctgaaaaaat cgaataatct ttagagaaac tttccaacgc tgaggcactt
    15121 acttttgtga aatctactgc tcctgactgt gtcaattcga tcagttcacg ttctccaccc
    15181 agttgtccat ctgggaaata ctggatctgt acggaaccat ctgttttttc ttttactaaa
    15241 cgcccaaatt cattcattgc atcaattact ggctggctgt tgcttgcata agcaaagcgc
    15301 aaggtaacta tctcatttac atctgcatct gcgacagttg ccacactaga attatttccg
    15361 cagccgctta gacctagcag acctaggcta agcgaacttg ctgcaaacag cagtgatgtt
    15421 tttttcatat gggtttcctt tcttttaaca ataagagacg agcaattctg tcaaacaaag
    15481 caccgttaac gactgtccat aaggcattgc tgtgattccg atattccgat aaaaatctaa
    15541 actgtccccc attcctgtcc cgattgaaac gttttgaact tctccttttt catcgatttg
    15601 ttcaagcaat ccttggatag cacgataagc gacttcttta tattcttgtg gtaaataacg
    15661 tttatggacc gctttcaaaa taccgtaagc aaatcctgca gttgcagaag attctacgta
    15721 agaagaagga tcatccaaca acgtatgcca taaaccgctt tcagactgat aagtttttaa
    15781 tgcacgcact tgtgcttcaa gtgtttcgat caataatttg cgcaatgcat cttctttcga
    15841 taattctaag atttcaatga tctctggaat agcaatcgtc aaccagcagt ttccacgagc
    15901 ccaaaatgct tcggcatagt tatgatttcc ttcaaacgtc cagccgtgat accacaatcc
    15961 tgttcgttta tccatcaagt atttgatatg gatcataaac tggtaacgag cttcttctaa
    16021 atattctggg cgattcaaca gtttcccgat ttttgccaaa ggaagcactg tcatcatcaa
    16081 tgtatcgtcc cacaactgat tcttattttc cggtccatat gtggcatgct gtaacccgtc
    16141 ttcatttgtc cgaggcatgt cattcatgac ccattctgcc cattgttcta aataaggcaa
    16201 atagcgtgaa tctttcgtgt cttcatacaa gtaagccata gtcaatagcg gtgccattgt
    16261 attgacgttt ttgggcggag cgccttcttc catacgatcc tcaaaccatt cattgaccac
    16321 ttgataagcg cgaggatttt ttgtcatctg gtaattttta taaataccat atagaccgac
    16381 accttgtggc caattccaca ctgtccagct tttgtcatct actttcagtc catcaaagtc
    16441 caacaaaaat tcaccattat cgtctttgat cgttgtcagg ttttcaacga gcaggtcaat
    16501 tttttctttc actaattctt taggtacaaa aatctcttct ttaataatca tctttacgcc
    16561 acctttttaa acgctttcat tttatatcct taatatagca gtaataccgt ccactataaa
    16621 tatcgctttt tgaacaaaaa ctgtctaaaa ttgacctaaa tgttaaataa caaaagtaac
    16681 tttcagttac ttggaaatta aaaaaagaac ttggagaaac aacgttttcc aagttctttt
    16741 tcatctattt tcttatcttt tttctacgct ttcttttgct tatccgttgt aagcatcaat
    16801 gatgatctgt ttcaattcag agatcaatgg ttctttcgga ttagctgtcg tacattggtc
    16861 ttcgtaagct aattcagcca tacggtctac ggttgtatct agtgtttctt gtgtgactcc
    16921 ttgagctttt agactcatgt tgatgccgac agattttcct aattcataaa cagccgttgc
    16981 taaagcttcg actaattcct ctgttgtttc gcctttcaga cctaagaatt ttgcgatatc
    17041 tgcgtaatct gtatctgcac ggaaatagtc atatttaggg aacatcgcat gtttttgtgg
    17101 atcttttgca ttatagcgga taatatgcgg taaaagaatc gcatttgtcc gtccatgagg
    17161 gatcccgtat tccccgccga ttttatgtgc gactgagtga caaataccta ggaatgcgtt
    17221 agcaaacgcc ataccagcca ttgtagaagc gttgtgcatt ttttcacgtg attccatatc
    17281 aggtgttttt actgattttt ctaaatagtc aaagactaac ttgattgctt gtaggctcaa
    17341 tccacgtgta taatctgaag ccatgactga aacataggat tcgattgcat gtgttaacac
    17401 atccatcccg gtatctgctg tgacagatgc tggaactgac atgacgaatt gaggatcgac
    17461 gattgcgaca tctggcgtca atgcataatc tgctaacgga tatttcacat gtgtttcact
    17521 gtcagtgatg accgcaaatg gcgttacttc agaaccagtc cctgatgttg tcgggataca
    17581 tacgaattgt gttttttctg gtttgtcgat cttatatgtc cgtttacgga tatccaagaa
    17641 tttttgtttt gcaccaaaga aagatgtgtc tgggtgttca tagaacatcc acattccctt
    17701 agctgcgtcc attgcagaac cgcctccaag agcgattact gtatctggct tgaagtctgc
    17761 catcagtttc gttcctgcgt atacggtatt tgtcgatggg ttcggttcaa catctgagaa
    17821 gacttcgatc ttcacatcat ttttacgtcg tgccaatact tcacgaactg tatcgcagta
    17881 accgaattgg accataccag ggtcacaaac gatcatgaca cgttcaacgt tttccatttt
    17941 ctctaagtac aatagagagt tcttttcaaa gaagattttt ggcggtaatt tgaaccattg
    18001 catgttattt ctccgtttcg ctacagtttt gacattgatt aagtttacag cagagacgtt
    18061 acgagaaaca gagttcttac cataagaacc gcatcccaaa gtcaatgacg gaatcatttc
    18121 gttatagata tcaccgatgc cgccttctgc agatggtgta ttcactaaaa tacgacaagc
    18181 tttcattcga agaccgaatt tgacatgcaa ctcttcgttt tctgaatgga taactgctgt
    18241 atgtcctaat cctccaagat tcaacatacc ttcacacaaa tcaaacgcat ggtccgtgtt
    18301 gttagctttg atcatggcaa gaactggaga aagtttttct cttgatagcg gataatctgg
    18361 gcctacgcct tccaattcag caatcagcat tttcgttcct tttggtacac tgattcctgc
    18421 caattttgcg atttccatcg cagaatgtcc aacgattgat ggattgaccg catattttcc
    18481 ttcgttcatc actgcatctt ctaatttttg caattcatct ggttttacga tataaacttg
    18541 gtgtgcttgg aattctgctt ttactgctgc atagatttct ttatccacga tcacagcttg
    18601 ttcagaagca caaatcattc cattatcaaa tgttttcgaa acgatcaaat cgttcactgc
    18661 acgtttaatt tttgctgttt tttcaatata agctggtacg ttacctggac ctacacctaa
    18721 agctggtttt cctgtagagt aagcagactt gaccattcca gctccacctg tcgctaagac
    18781 gatcgcgata cctggatggt tcattagcat agaagtcgct tcgatcgaag gatgttcgat
    18841 ccattggata caattttcag gagctccggc agcgatagca gcatcacgta ccactcgtgc
    18901 tgcttctgca gaagattttt gggcacttgg atggaaagca aaaacgattg gatttcttgt
    18961 tttcaacgcg atcattgatt tgaaaatcgt tgtggaagtc ggattcgttg ttggagttac
    19021 tccgcaaacc acaccgactg gttcagcaat ttcaatcaac cctttttgga catcttcatt
    19081 gatcacacca actgttttat catgtttgat attatgccaa atatattcgg acgcatacat
    19141 atttttgatt gctttatctt cgtaaacacc gcggcctgtt tcctctacag ccatcttcgc
    19201 taatggcata tgttgatcca aagctgccat tgccatttca tgcacgatat ggtctacttt
    19261 ttcttgatca aagctttcca tttctttcaa tgctgcattt gctttttctg ccaaatcatc
    19321 aatcattgct tgtacgtcaa ttgtcttcgt tttttctttt tctattactt tagccatcat
    19381 tatcctccta atgggtcatg gttatttgtt aaataattca catgtatatc atacaatatt
    19441 tatttttcta tgtaaacact tttttgtgat ttttttcaca gttattttta aatataaaat
    19501 tgctttattt acaggtgttt gtatagaaaa atatataaag ttatttatta ttatttatat
    19561 gacacacccg attattcaat attgagcaat atatcccatg ctcttatctc tttctttttt
    19621 gaataaaaaa tgaggtagga catctttctg ccctacctcg tctttaaata agctttgata
    19681 cttatttttc ttccttctct tctgtctctt ccagcgtttc tgttgtttca gtttttactt
    19741 caactgtctc agctggttct tctgttgttt caacagtagt cgttgcatca tttgttacga
    19801 cagtccctgg tttgaccgtt ttgattgctg cacgatcgta ctcaaggaag atcccttcgc
    19861 agtctaaaac aactgtccgt ttgtcattgt cgatttctga aacaactcca tgtaatccgc
    19921 cgatcgttac gactgaatca cctggtttca tgctatctaa caagttttga cgttcttgct
    19981 gttgtttctt ttgcgaacgt gacataaaaa accacattgc taatagcgcg ataaacatca
    20041 caagcattgg taatgttccc atttttttca cctcactaat tctgtatcta caaaaaacac
    20101 tttagcagaa aaatgctgat aacactagtt ttttacttaa tttttaaaag attttccttt
    20161 agaagctttt tgcgttttct ttattgaacc cgtattcttc aaaaaaggct tctctaaatt
    20221 caagcaaatt atcatccatg attgcttgac gtacttgttt cattagattc aacaagaagt
    20281 ataaattgtg gtaagaagtc aaacgaatcc caaatgtttc atcacatttg atcaaatggc
    20341 ggatataggc acgtgtatag tttttacatg tataacaatc acatttctca tcaattggac
    20401 ggaaatcatg ggcatatttc gcatttttca ctactagacg gccttgagaa gtcatacatg
    20461 tgccatttcg agcgattcga gtaggcaaga cacagtcaaa catgtctact ccacggataa
    20521 caccatcgat caatgaatca gcagcaccga cacccatcaa gtaacgtggt ttgttttcgg
    20581 ggatcaacgg tgtagtgaat tctaatacac gattcatttc tgatttaggt tccccgacag
    20641 ataacccgcc gattgagtaa ccagggaaat ccatcccgac tagatctttc gcgctttgac
    20701 gacgaagatc ttcaaaccct gctccttgga tgattccaaa taatccttga cggtctggat
    20761 tagcatgagc tttcaatcct cgttctgccc atcgagacgt acgttctact gattttttca
    20821 cgtagtcgta actttcatcg aatggtggac attcgtcaaa actcatcatg atatcagagc
    20881 ccaatttatt ttggatgttg attgcttttt ctggtgataa aaacatacgc gaaccgttca
    20941 agtgattttt gaaatgcacg ccttcttctt cgatattccg catatcactt aatgaaaaaa
    21001 cttgaaagcc gcctgaatct gttaaaatcg gttgatccca gttcataaat ttatgcagtc
    21061 cgccagcttc ttctactaag tcttcaccag ggcgtagcca taaatgatat gtattactta
    21121 aaataacgcc agcacccatt tctttcaact cttctgggga cattgttttt actgtagcta
    21181 atgttcctac tggcatgaac attggtgtgg ggaaagttcc gtgtggcgtg atcaattccc
    21241 ctaaacgcgc gccagtatgt ttttctttct tgattaaacg atagcgaatg gcaggttgcg
    21301 tcattcattt ccctactttc tatataaaat atcttgacaa ctcccaccat cataatcgtt
    21361 tcaagtgctt tttgcaagtt ccctcggctt ccttctactt tatttaatat tctttttttt
    21421 atcctttttt tatgaataac aaaaaaaaga aaaacattat gacagaaata ttttttgata
    21481 catggtatat tggtgttatt aaagaaaagg agggaaaaat catgcaatct tctgcagaat
    21541 taaaacgcca agcaaaagag agtctaaaag gacgttgggg acaagctgtt ttactaaatc
    21601 taatccctac attgattggt attgctacta ttcttcttat tatgattcct attatcttgt
    21661 ttgccaccac catatatgat ggatcagcga gcgtctcaga agcgacaact tcaggaggcg
    21721 gtagttcagg aggcggtggc ggaattattt ccagcatcat tagcgctttg ttcatgagcg
    21781 gtatttcttg gacttatctt gatattttac gtggacaaaa agatcagatc gaaccattca
    21841 aagatgcatt tcgtggattc aaaggtttgt ttatcggcgg agtgatcagc ttagctgtgc
    21901 tgatctcgat ctttacaaca ttatggggtt tgctattagt tattcctgga atcattaaaa
    21961 gctattctta ttctcaaagc tattttattt actacgatat cgtccaagaa acaggacaaa
    22021 cacctaaagt actggatacg atcactgcaa gtcgaaaatt gatggatggt tataaaggac
    22081 aacttttctg gttagatctt tcatttatcg gctggcatat cttagcatta gcaactgctg
    22141 gtattggtta tctatggctc agcccataca tttctgcaac aaaagcagca ttttatgaac
    22201 aattgccaaa agatatttaa aagagagtgt gacttgtgac cgagggggtg gcaggagaac
    22261 accgtttatc gagaggcagt aaaagaaaca ttcagcccat tagcaaaaag gaggtgtgac
    22321 aacattcatg tcacacctcc tttttgtata gttccagtaa aaagaaaatc aacttagaaa
    22381 cagttaatca ttcctcttct tctgcttccg gtaatcgatg gaacttgatc agttcattca
    22441 ccgcgattgg tacgatagat aaaatcccta ccaagaacca gtgattcaaa ctgatgtgta
    22501 ctaggccgaa tagttcttgc gtaaatggca gcaaagcaac agccgttgta atagctaatg
    22561 ccagtaacgc catttctacc aaagctttat tggtcgctaa attgactttg aagatcgatt
    22621 ggctgctgcg tacattgaac acatgcagaa tcgatgaata agctaagatc aagaatgcaa
    22681 cagtttgacc gacatgctga cttgcgtcta cccatgggct gatagtatcg acataactac
    22741 ctaaataata accaaacaag gttacgatgg tgaagacccc tgcatttatg gcaatttttt
    22801 gcaataaacc tcgagcaaag atcccttcat ttttcggaat gggtggctga tccatgatgc
    22861 cgtgttccgc tggctctctt cctaaagcaa atcctggaag tccatcagca actacattga
    22921 caaacagtag ttgcacagct gtaaatggtg ctccccatcc taacagcatc gcaatcaaaa
    22981 caataaagat ctccgaaata ttacagctta gcaaaaagtt gatagcttta cgaatattct
    23041 gataaaccga acgtccttga ctgacagcgt ctacgatcgt cgcaaagttg tcatccgtca
    23101 aaatcatatc tgcggcactt tgcgccacat ctgttcctgt gatccccatg gcacacccaa
    23161 cgttacttgc tttcaacgct ggtgcatcat tcacaccatc cccagtcatc gctacgactg
    23221 cacccgtacg ttgccaagat ttgacgatcc ggatcttgtc ttctggcgtt acccgagcat
    23281 agacagagag agctttgaca cgcgcatcta attcttcgtc tgataattga tgcagttctg
    23341 atcctgaaag tgcttctgac ggatcattca agatacctaa ttctttcgct atggcactgg
    23401 cagtcaccac atgatctcca gtgatcatga ctgttttgat gccagccttt ttcgctcgct
    23461 tgattgctcc tttactttct ggacgtggtg gatcgatcat tccgatcaga ccaatcaaac
    23521 gtaagtcttt ttccagtgct tcagaagaaa tatcttgcgg ttcttcgtca taagtagcat
    23581 aaccaacagc aatcactctc agtgcccgtt taccaaagcg atcattaacg atcgccgctt
    23641 gttctacatc gcctgaacta aagcgcggag caagaacatc gaatgctcct ttggtgattg
    23701 agatgtattt ttcgcccatt tggtgaactg ttgtcatcat ttttcgttca gaatcaaaag
    23761 gaagctcgtt tactcgtgga tatttttctt ctaattctgc ctttgtatga taattctctt
    23821 caacagccct tacgatggct acttctgttg gattaccttt gataacaagg tcgtcatctt
    23881 cttgctcgac cgtcacatct gtacatagtg cagccatttt caatatttcc atcgcgctgt
    23941 tcgtcattga atcttcgata ttcgttactt catcttcttt gctccatacc cggcggacgc
    24001 gcatttcatt ttgtgtcagc gttcctgttt tgtctgagca gataacatta gcggttccta
    24061 gcgtttcaac tgctggcaac tgtcggatga tcgcatgttt cctcgccatc ttctgcacac
    24121 cgtatgcaag tgtcaacgta acaataacag tcaaggtttc cggtacagct gcaacagcca
    24181 aggaaatcga agtcatgaac atctcaagga gtggttctcc ttgcagttca ccaatcacaa
    24241 atactaaagc cgcagccgct aaagcgatca tactgattcg tttgcctagc tgattcaatc
    24301 ggacctgtaa aggtgttttt tgcatagtat tttcgttcaa aagatccgcg atcttaccca
    24361 tttctgtagc catgccaatg gcagtgacga tcgctttccc acgacctgca gcaaccgtac
    24421 atcctttaaa taccatatta agttggtcac ctaatgactc atcttcttca gcaataaatg
    24481 ctgcatcttt ttcaactgct tcactttctc cagtcaacgc cgattcttct acacgtaatt
    24541 gtgatgcttc gatcaaacgg gcatcggcag aaatagcatc accggactct aaaacaagaa
    24601 tatcacccac taccagttgc tctgactcaa cagtggtcac tttgccgtct cgtatgacag
    24661 ttgccgtttg tttattcata tcttgcaagg attccagtgc tttttctgcg tttccttctt
    24721 ggacaatcgc caataccgag ttgatcacaa cgatagcgat gatcagcagt ccttcaaata
    24781 aatccccatg ttctgtagca aatgcggtat aaaaggaaat caccgctgct acaagaagga
    24841 tgatcgttgt aaaatcggac aagcttcgaa agaatttttt gatcatcgat tctttcggcg
    24901 cctcatcgaa tttattcaat ccctgctttt ttcgccgttc atccacttct tgtgaagaca
    24961 acccagtatc tggatctgct tctgttacct tctttacttc ttctattgtc tgcttgtatt
    25021 cttccatcct attcctcctt taaaatcttc gtacaaaaaa aggtacttat tttactataa
    25081 catccttttt ttatttactt caaataaagg gcttatcgat tatttgttaa gaaagcgtaa
    25141 aatttaaagt gcttatattt gcaaacgcta ccaaaggatg ctattatata gatgtaagga
    25201 aggaggatac catcaaatag acagaagaat ttctcgatag aacagtgtaa gcaaacaaag
    25261 ttgttagaag tacaggtttc acccagacat gcgtgtagag tacaaaccta taaatagtga
    25321 atgcgtatgg atgtttggat cacgaagtat ccgaaacaag aagtaatgcg atacgtaaca
    25381 attgaataac gtaaacgtct gaaaattgaa taggaacaag agatgtttca aatgttgaag
    25441 atgtaacgaa aacaaaattt ttgtttaaca tgaagaaatt caacaccgtg ctttgcatgt
    25501 atgatggaaa gttacaatca tttctgttta tttgaatcct tccgaggtac aagaaagccg
    25561 agcttatcga gagctcggct ttttctttgt cataaaatct ttgtaaagct tttttgttat
    25621 tcaaaactag cggcttttca taaccttatt tcacgaacat cgcatctcca aaactaaaga
    25681 aacgatagcg ctcttcaatc gcatgctggt aagcagataa agtcaattct cttcctgcaa
    25741 aggcactaac taacatgact aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt
    25801 ctaccacttt gaattcatag ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt
    25861 tgatttctcc attaaatttc gttccaatcg tttctaatgt tcgaattgaa gttgttccga
    25921 cagccacgat tttcccgcca tttttacgta cttggttcaa tcgctcagcc gcttcttccg
    25981 tcaaacggta gaattcacta tgcatatggt gttcttcaat tttatctacg ctgacaggtc
    26041 ggaatgtacc taaccctaca tgtagtgtca gatagactaa ttcaacaccc tttgcttcga
    26101 tctcatcaag taattctttc gtaaaatgca atccagctgt tggcgctgca gcagaaccat
    26161 tttctttcgc atagacagtt tgatagcgat ctgggtcttc cagacgttct ttgatatatg
    26221 gaggcaatgg catttctccc aatgattcaa gaatctctag aaaaattcct tcatactcaa
    26281 agtcaatgat ccgtccgccg tgttccaatt cttctttcac tactgcttgc aatctgccat
    26341 caccaaaaga gattcttgtc ccaactttgg cacgtttcgc aggtttgatc aatgtctccc
    26401 acgtatctcc ttgtgtattc gttagaagaa ggacttctaa atgcgcacca gtatctggtt
    26461 tttctccata taaacgagca ggcagaactc gtgtattatt cataactagc gcatcaccag
    26521 gctgtaactc ttcaatgatt tcatggaaat gtttatcttc catctctcca gttttccgat
    26581 ccaaaacaag taaacgagaa tgatcgcggt cttttaatgg tgtttgtgcg attaattctt
    26641 ccggcaagtc aaaatcaaaa tcttctgttg ttaacatatc gtcactcact ttctatcatg
    26701 tgcttcatca ttctatcatg tttttcgttc tgttggcaaa atcagcactt tttcacttct
    26761 attttttgtt ttcctaaata aaaaaactac cctatttgta ttttttcatt gttttaggca
    26821 tactagtaat agatagttct tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt
    26881 tcaaaatgtg tcaaaaattt ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc
    26941 atttgaaaag ggtgagttca tttgttttat cggtacgagc ggaagcggaa aaacgacgac
    27001 gatgcggatg atcaaccgaa tgactgatcc aacacaaggg aaaattttga ttgatggaaa
    27061 atccatccaa gaaattgatc cagtggaatt gcgacgtcag atcggctatg ttatccaaag
    27121 tattggattg atgcctcaca tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt
    27181 gaaagtcagt caagaagaac ggaataaaac agctgaaaaa atgattgatc ttgtggaatt
    27241 gccccgtgag atgttagatc gctacccaca tgaattatct ggtggtcagc agcagcgtat
    27301 tggagtcgtt cgtgcccttg cagcgaatca agatatcatc ttgatggacg agccattcgg
    27361 tgcgcttgat ccaatcactc gtgattcctt gcaagacctt gtcaaagatt tacaagagcg
    27421 tttgggaaaa acgatcgtat ttgtaaccca tgacatggat gaagcgatta aactagcaag
    27481 ccgtatcgcc atcatgagtg aaggccgtgt gatccaattt gatacaccag aaaatatttt
    27541 acgtcatcct gcaaacgatt tcgtagaaga gttgattgga gaagatcgcc tactacaagc
    27601 caagccaaat gcgacaagag tcggcgaagt tatgctaaat acagcaatca cgattacacc
    27661 ggaaaaatca ttacaagaag caatcaaact gatgagggaa aaacgtgtag atacgttgtt
    27721 agttaccgat aattcgaatg tattaaaagg atttatcgat gtagaaacgt tgaataaaaa
    27781 acgaggaaaa gtatccagtg tcggcgacat tttgaataaa catgtttttt acgtaaaaga
    27841 atccgcctat cttcgtgata ccttgcaacg cattttaaaa cgcgggctga aatacgtgcc
    27901 ggtcgttgat gaacagaaca aagtcgtagg tatcctgact cgtgcatcat tagtggatat
    27961 cgtctacgat gtgatttggg gagaagatcc cgcaactact gatgttgcag agtcagctac
    28021 tcctgaagaa tctaaggagg tctgacctat gcagcagttt tttcatcagc acggttcaga
    28081 aattctctca aaaggttttg aacatcttta tatttcagga cttgctttga tcattggtat
    28141 catcattgct gtaccgcttg gtgtcttact gacacgtttt ccaaaagctg ccacgattgt
    28201 gattggctta accagtgcct tgcagacaat cccttctctt gctttattgg cattgatgat
    28261 cccgctattt ggcgtaggta aattacctgc gattatcgca ctctttattt attcattatt
    28321 gccgatttta cgtaatacgt acattgggat gaaaaatgtc gattggaatt atcgggatgt
    28381 ggcaaaaggc atggggatga ctgagttcca atccattttt tccgttgaat tgcccat
//
LOCUS       DACEPW010000016        26613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEPW010000016 DACEPW010000000
VERSION     DACEPW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.016"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(332..1276)
                     /locus_tag="IW819_000536"
     CDS             complement(332..1276)
                     /locus_tag="IW819_000536"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1243666.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1572..2516)
                     /locus_tag="IW819_000537"
     CDS             complement(1572..2516)
                     /locus_tag="IW819_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1243667.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2737..3951
                     /locus_tag="IW819_000538"
     CDS             2737..3951
                     /locus_tag="IW819_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1243668.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3973..5988
                     /locus_tag="IW819_000539"
     CDS             3973..5988
                     /locus_tag="IW819_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1243669.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6071..7444)
                     /locus_tag="IW819_000540"
     CDS             complement(6071..7444)
                     /locus_tag="IW819_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1243670.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7470..7754)
                     /locus_tag="IW819_000541"
     CDS             complement(7470..7754)
                     /locus_tag="IW819_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1243671.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7756..9713)
                     /locus_tag="IW819_000542"
                     /pseudo
     CDS             complement(7756..9713)
                     /locus_tag="IW819_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
     gene            complement(9892..10197)
                     /locus_tag="IW819_000543"
     CDS             complement(9892..10197)
                     /locus_tag="IW819_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1243672.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10286..11317)
                     /locus_tag="IW819_000544"
     CDS             complement(10286..11317)
                     /locus_tag="IW819_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1243673.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11524..12399
                     /locus_tag="IW819_000545"
     CDS             11524..12399
                     /locus_tag="IW819_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1243674.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12551..13393)
                     /locus_tag="IW819_000546"
     CDS             complement(12551..13393)
                     /locus_tag="IW819_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1243675.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13485..15362)
                     /locus_tag="IW819_000547"
     CDS             complement(13485..15362)
                     /locus_tag="IW819_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1243676.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15359..16780)
                     /locus_tag="IW819_000548"
     CDS             complement(15359..16780)
                     /locus_tag="IW819_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1243677.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16783..20172)
                     /locus_tag="IW819_000549"
     CDS             complement(16783..20172)
                     /locus_tag="IW819_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1243678.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20429..21820
                     /locus_tag="IW819_000550"
     CDS             20429..21820
                     /locus_tag="IW819_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1243679.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNMIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21893..22540)
                     /locus_tag="IW819_000551"
     CDS             complement(21893..22540)
                     /locus_tag="IW819_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1243680.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22548..23234)
                     /locus_tag="IW819_000552"
     CDS             complement(22548..23234)
                     /locus_tag="IW819_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1243681.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23240..24313)
                     /locus_tag="IW819_000553"
     CDS             complement(23240..24313)
                     /locus_tag="IW819_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1243682.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24535..25209
                     /locus_tag="IW819_000554"
     CDS             24535..25209
                     /locus_tag="IW819_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1243683.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25214..26554
                     /locus_tag="IW819_000555"
     CDS             25214..26554
                     /locus_tag="IW819_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1243684.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
BASE COUNT         7817 a         4991 c         4852 g         8953 t
ORIGIN      
        1 agtttttctt actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt
       61 taatcaaata tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt
      121 taattaacca cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt
      181 catatgttca tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt
      241 tgcttaccta ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt
      301 tttaggattt ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta
      361 aagcacttgt tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa
      421 cttcacgaac accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt
      481 cgtattcacc agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag
      541 cttttacgat acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa
      601 tgatctcata agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa
      661 tacgatggtc tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc
      721 atgcagcaac ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt
      781 caacttttaa tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag
      841 taccgataac acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat
      901 ctactgggtt tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta
      961 cgatttggcg catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt
     1021 tttggttgat accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac
     1081 ctgaccatac agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt
     1141 ccccttctgc tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt
     1201 tgatcattgc ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac
     1261 gacttgtttt tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa
     1321 agtatagtct tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt
     1381 tttgatggaa acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat
     1441 atcccctgat aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc
     1501 aacgatataa taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa
     1561 agattttctt cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg
     1621 aataatttga ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac
     1681 gaaaaactcg tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt
     1741 tcttgaaagg aagcatcaaa atacattttc aatttattac taagatagac cggattgtag
     1801 ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct
     1861 aatactctaa gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct
     1921 aataaatgag tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta
     1981 tgactgtatg tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt
     2041 gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt
     2101 atcaaaaaag aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat
     2161 aaggtttctt cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt
     2221 ctttgagtag cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct
     2281 ccattaataa tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt
     2341 gtatgctcgg gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga
     2401 aatagatccc ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca
     2461 ttgatattca ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa
     2521 aatgtctaat tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct
     2581 tattagttca cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt
     2641 ctatggttgg gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact
     2701 atatttttga taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg
     2761 aaattttctc tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca
     2821 attcccagta tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg
     2881 actgctccct cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa
     2941 atcggacaaa aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc
     3001 cccctcttct cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc
     3061 tttggattga tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa
     3121 cgagcctcta tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca
     3181 tttgttgcag gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc
     3241 agcttaccta ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt
     3301 caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt
     3361 tatggtattt ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc
     3421 acagtcctga cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc
     3481 atgcaattgg caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat
     3541 cgctatatgc ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact
     3601 gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta
     3661 ttcattcctt acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc
     3721 aatacttcaa tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg
     3781 ctctttctct cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt
     3841 atcttgctgt tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag
     3901 acagtgataa gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg
     3961 aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac
     4021 tttgagatcc gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg
     4081 ccagcgtact ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt
     4141 cttgatcaac cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat
     4201 ggacaaaaac atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt
     4261 atctttgtag gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa
     4321 tcagatattg gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg
     4381 ctttatgtcg atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat
     4441 ccagtcactg aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat
     4501 ggctgtgtag aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga
     4561 gagtccgatg tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca
     4621 agaaaaatga aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact
     4681 ggaatggttc aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt
     4741 tcaagcgatt cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta
     4801 tgtagtgatt tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat
     4861 gcgtatcaat cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga
     4921 acgatattag cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac
     4981 tattcgatct tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa
     5041 tttttacgca tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg
     5101 aatgaattag ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa
     5161 atggataaag aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc
     5221 atgatcactt gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact
     5281 catctctatc aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat
     5341 gaagaacttg gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat
     5401 gcgaatattt tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta
     5461 aagaatgtct tattgaatga cactatcaca cagattacta ctccatattt caaattctat
     5521 gaacaagatg ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat
     5581 tattggggag ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact
     5641 caaacaggaa atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat
     5701 gcatggggag caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca
     5761 aagccaggtt atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat
     5821 tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg
     5881 attacttctg atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta
     5941 gcaaaacagc cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg
     6001 cttgcgacat atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt
     6061 tgaagtttgc ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa
     6121 agctagataa tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac
     6181 cgtattctcc tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg
     6241 tagaagaagc gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa
     6301 tcaataagct attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc
     6361 cgaagacacc agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg
     6421 tagtttgcaa gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa
     6481 agccaattaa tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt
     6541 tagaatcggg tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca
     6601 ccattcgaac cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca
     6661 gtgcaaagat tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag
     6721 ctaacaaagc aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat
     6781 ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc
     6841 caaaaaactt tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg
     6901 caacttgatt gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag
     6961 ttaatagtgt tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc
     7021 ccgcgcttac taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa
     7081 gaaaaatgaa tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta
     7141 gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat
     7201 tgctaggaac tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta
     7261 gtgaattact gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga
     7321 tagttccttg gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa
     7381 tcaaagaaac gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat
     7441 ccattgatag ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt
     7501 tcttttactt cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata
     7561 gaagttagcg tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat
     7621 acacttccaa catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca
     7681 agtgcttgtt tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact
     7741 gcaaaaattt tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt
     7801 tgatgcgtgt aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc
     7861 caaagcccga agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg
     7921 atgggctgga ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac
     7981 ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat
     8041 aaacgcacca tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt
     8101 attttctgat aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg
     8161 atccgataat tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc
     8221 cttcttttct aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc
     8281 tacgccttct tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg
     8341 cattgaaaat cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc
     8401 caagtcagtc atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag
     8461 aggtacagta gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc
     8521 tgaattagtc tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat
     8581 caatgaagag ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc
     8641 ttctgcgtcc gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc
     8701 ttcagggatg ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc
     8761 gtataggaaa tacgaaaata ggcagggacc aagtgataaa agacattcat caataattca
     8821 cgaaacgaag caaattctac ggccatcagg aattccatgc gatggatgac ttctgaagaa
     8881 cattccagca aaaaatcaag tgcaggctcc tggacatctc cttcaacgat cgatgctaag
     8941 caaagagaga aaaatataat ctccgaattt tgatgttcgg aatagccaag gccagtcaat
     9001 acttgtcgac taatgtcagt gactgcaaga gaagccaaca gttcagcatc ttttaccagt
     9061 tccatggatg tttttccaat tcgaatggtt gtcaaactca aataataagc taattcatcc
     9121 attcgatttg gtgcgaatcg aagatctgat agtgaaatcg ttttatctag caaatctcta
     9181 actttggcgt aataatcaat ggaaagggtt gcggttaaat acgtgagccc aaatcttcca
     9241 gcaagtgact gcatcaaacg agcagtgcga ttttttgctt ctcttcttaa tattgcttct
     9301 gaccctagca gcctaaaacc agttttccta gtatacgaca gctgacaatc ttccacttct
     9361 aattcatttc ttatcttttt gatatctgac aggactgtcc ctttagaaac ctgaagaaaa
     9421 tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat aaaccatcaa ataaagaaga
     9481 tctcttcgct cttgttcggt atacacagct tcaaatcgag taatatccaa aaatttttct
     9541 gcccagtcga ttgctcggaa tttctttata cggatacggg tatcatccac atattcggca
     9601 aaaggagtta attgtttgat cgcctcgctt gtatttactt cgatgatctc tgctgctttt
     9661 tctagactga tttctcttgg tgttttcagg agataactaa ttttgtagtt cacagcgctg
     9721 ttcacctcct acttatattc tagtatggta gcgcttttaa atcgaggggt gtttggtaca
     9781 acttacagtt ggattttttt tacgactgta ccgaacgatt tctatcgaat ggatacagaa
     9841 aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg atttcgtccg atcaatgatg
     9901 atcatgttta ttttttgagg tcagttcttc aaatttttct ttgacatctt caaccgcttt
     9961 ttcgatatct ttttcaaagt gattgggctc attaggcttt gtttcctctt ctacagaatt
    10021 tgaaccagat aaagctgttt ctttattttc tgtttcacca gagtttgtgc cagctaatga
    10081 ttcttctttg tcttctgttt caccagaatt agttcctgct aaagccactt cttcttctgt
    10141 tttaggggcc attgaacctc ttaatcgttt gtgtttttca ccgtcataat ttgtcatgtt
    10201 ggttcctcct agtagttcta tactttttca tcgtacaata agacagttta tgcgtcaaaa
    10261 aacaaacccg attcttacaa atatgtcata tttttccttc tgattcttga atgcttttga
    10321 ttccttcttc caaaattttc atttgtaaga ttgtttcttc atctttgatg actttttctg
    10381 cgccattaac taatgtctcg taaatccctt cgtagacacg gctgtaatca ccaacctcgg
    10441 aaattacttt ttcttcatga taactccctt gttcatctat ataggtcaat acaccataat
    10501 gttcgggcaa atcgatacca aagtcttcat gatcaggcat gtaaaacatt ttcagatgtt
    10561 cttcttgtcg atcttttgtt tgtttcacga acatcccttt ttttccgtaa acgacgaaac
    10621 ttggacgttc tttgatgcgg aaataactgg attttatcga aattttcata tttccataga
    10681 aaaaatccag atcgaagtaa tcattcattc gtcctttccc taaaagttga cgtacatcat
    10741 agctgatatc atctggttcg ccgaaataag aaagaacttg atctactgta tgacaagcat
    10801 gtccatataa atagctgttc gcagtagaaa attcatgcgt attttctggt acttccggtc
    10861 tgaaataatc ataatgcatt tctacttcta aaatatcccc taattttccg ctttcgatta
    10921 ctttttggac tgtcaagaag tcagaatcga accgtctgtt ttggtaacat tggataaata
    10981 gacctttttc ttttgctaat gcaaataact cttctgcttc ctttgacgtt tctgcaaaag
    11041 gtttctctac tagtacattc ttaccatgtt cgagtgccat ttttccatat tcataatgaa
    11101 attgactagg tgtagtgatg acaactaatt ggatctcagg atcatcccaa agatcagaca
    11161 gctgatcagt atattcgatg tcttcgaatg ttttccacac tggttcagat ggggaaaaaa
    11221 tttttttgat attgattttg ttcgtaagtt ttcttgaaaa aggcaaatga tagcgatttg
    11281 tactctttcc attgccaaga taagcgattg taagcatgag tggtgcctcc ttttattgat
    11341 gttttcagta tatcctagaa aagcgcagaa agtatttata atagctattc tggtaaaaaa
    11401 gttcagaaaa acacttgtta tcattcggtt tggtgaaatt aatgttcaaa gtaaggataa
    11461 aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta agatagaatt tggaggtcag
    11521 atcatgctta ttgaagaaaa actaacaaaa caagagttgt ttaccacgac agagaaacgg
    11581 attgtcgatt acatccgtag gaatatcgaa gctgcagtct acatgacgat tgaagaatta
    11641 gcaaaagcta cgtatacttc ccattcagct atcattcgtt tatgcaaaaa aatgggattc
    11701 agcggattta aagaattccg tttcgagctg gggcgggaag ttcatcaatt gattgctcag
    11761 ttcaatcaaa cgattgatcc taattttcct ttttcaagtg aagatagcac tcagcaaatt
    11821 gcaaagaaaa tggcggattt atcgatccag tcaataaaaa aagcacaatt gcagattgaa
    11881 catgaagatt tagatcaaat cggtaaagta ttgaacaagg cacagcgaat ctttcttttt
    11941 gcaaaaggtg attctcaaat cacggcaagg aaattccaga ataaaatggt caagttgaat
    12001 aagtttttga tcatggctga agaatacagt gattcttctt ggaatgcagc caatttgact
    12061 agcgaggatt gcgccatttt tattagctac agcggtcgca tccatcatta tgaacgaata
    12121 atgacctatt tgaagcatgt agaggcccct accttgctga ttaccggaaa tcagcattca
    12181 gaaatggcca aacaggctga tatgtgtctt gttatatcgc aggaggaata tgattttgct
    12241 aaagtagcga ctttctcctc tcaaattgcc ttcgattacg tattaaatac gttattctca
    12301 gtgatatatt ctcaaaattt tgaagagaat atccgcaact tgaaaaacaa acaagaattg
    12361 atgcaaaaag gccttcttaa agaatatcgg aaaacttaag aaaagatcct catttagtaa
    12421 taagaaagat agtgtttgat aggctctctt tgatatcatc ataaaaacag gctgaagttt
    12481 tctttttcaa acctcagcct gtttgtttac ggaacgattc gaatagatat attatagaaa
    12541 tgaggaatag ctaacttctg tttcttattt tgaacgattg acgaaaaaga tatataagaa
    12601 ggaggaaaat cagtatacat gcagctaaca atccatacag gaaaaatcgt ttgttctcgg
    12661 atactttctg tacttctttt tgaacttctt ttgcatgata aggaacccgg tgccctctga
    12721 caagtaagcg atgactgttg accatataag gtgtacaagt taggagtgtc acgaaatcct
    12781 gccctttttc gattctcaga tcttttgtat cagtcggttc taccgtcttg atctgatcga
    12841 cttgataagc gagtgtccgt ccattgattt cgatatagaa agaatcacct aaagtcagtt
    12901 tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg atggctggaa ataacggcat
    12961 gtgtattttc tccccccgta ggataagacg ttccttccaa taatgaggca cctttttcta
    13021 gcagcacagt attcgtttca tcaaaaattg gcaaatggac attgatctca gggatcgtca
    13081 aaataccgat cgtatgtttc tcaaaatagg ttctatcctt tttcgcaggt tcttttttct
    13141 tagtaaaggg atctgcacct ggatttccac cttcttttgc taattgctgg tttttctttg
    13201 tcatgttttc ttggatttta gccatgactt cttcattttc tttgaccgct tgttgctgat
    13261 aatgagagat gatctgctga tccagataat tattcagcgc gtcgctaaca aatggataag
    13321 ctagtatccc tattcctgag agaagcaaga aaagcatcag cagatctaga agtttccttt
    13381 tctttggttt catttgtgtt ctccttgtga tgactgtttg ataataaaag aaaaaggagc
    13441 atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa aagactaaat ctgactgtgc
    13501 ttgcgtctag caaagtacag tccagcaatc agtagaagga ctactcctgc accgatatac
    13561 acatagattc ccttaccgcc tgttgaagga agtgtacctt tgtgtttatt tggtattttt
    13621 tcaggagaaa tcaactgtcc tgctgttaca taagattgtt catcgatagt aaatgctaca
    13681 cggtttgtta atggaacata tttttctggc gctttcgttt cttccagata gtacgtgcca
    13741 tattttagac ctgtcacatc gattaaaccg ttacttgtgg ttgtaaaaac cgttgctgat
    13801 tctttcgccg atacccagct gacggctttt gtggatgggt cgatcgataa atatttcgct
    13861 gtgtcactat cttgatcacg aacgacgaat tctgctccag caagtgtttg gtctgatgta
    13921 acgtcaccat ctactttaac aaatcgtttg cccccagtaa cgacatcgac tgacggtggt
    13981 gtttgatcat ttgtatgccc gttatcgaca tttgcttggt tagaaaatcc tttggttgga
    14041 tctgcttttt cgttcaaatg catatagtaa acgaatttca atgtaccgcc aggagttaat
    14101 gaaggaatat aattcggatc aactgaaacc gtgaatccgt ctgtttgctc agtgacagaa
    14161 taatttactg ggtcgatttc tttatttcca tcatataagg cataagcata cgttcctgaa
    14221 gaatcattat caaatgttaa agcagcgtca tgagtatcga taagtttgaa tgttgtgtac
    14281 ttgttttgcg ttccttcttt atcagcgata cctaatggga tattgacaga aatctcatat
    14341 tgaatttttt caccgattgc gacatctttt ccattcaatt gaggtgttgt tttatcaact
    14401 ttagacgtat catttttgat ggtcttttct actggtgttt ggctatttgc caggatctca
    14461 aaaggcgtga ttgtttgctt ttcaatcatt tccgcattat caggagcttt tacttcttct
    14521 aaattatatt ttccaacttc taaatgatta acgatcaact ggcctttttc aatagatgcc
    14581 tcggcaaagt cattgttgcc gatgtcataa gaatgaccag taatgaaatg tttggctttg
    14641 gtttgatcag ttgtccaagt gtacaatcca tctgtgacac tttggatgta ttttttgacg
    14701 cttggtgttc cttcttcttt agaaataata aattctgctc catttagtgc ttcgttttcg
    14761 gcagtaccga tttttgtaac tttcaacgtt ccatcattac cgactgtatt tttagggtag
    14821 agatggatag tatctagttc ttctgtcccg tatttataag agccatctgc ttgtttgatc
    14881 atctcatata cagggaaagc taaaaccatg tttgcggcag ctgacactcc gtcttttggt
    14941 tcttctttga tcgtgtagac tgcatctttc ccattttgtt ttttaggtaa agataaagtg
    15001 acatttccat cagcatctgt cgttcctgaa gcaactggtg taccaggagt caaagactgg
    15061 actgcttgtt ttgctgcatc cacggacgct cctttatctc gttgcgcata aaattcttga
    15121 gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt ggtattgatc gaattcgctc
    15181 atttctttcc cgctgttttg gattaaagga tcgggtaaat cagtcatttt ctttttatga
    15241 aggatgactt gagctgctgt ctcctctgca aacacttttt ttgcgccgaa gctgtttgtg
    15301 agtaatggta aaataaggaa aaggactcct aacataacgt ttattttttt atgatttttc
    15361 attttttcac tcctgttctt tttttgataa acaaccagat tgcaaagctt gcgaatacca
    15421 taccaacaac gcctaaaagt gtatttttcg tttcgtttgt ttttggaaga aatggacggc
    15481 tattttcttt tttattgact actttcaata tactgtccgc ctctttatcc tcagaaagaa
    15541 aagttgaaat ggtaaacgga atttctgttt cacttaaaat ataaccagaa ggtgcttcga
    15601 tctcttttaa tcgataactt cctgttttca acccggaaat tgaaaaagcg ccatttttat
    15661 cggaaatcag gtgaagcgca tctgattcgt tctttgtcca cttgtatccg ttggctgttt
    15721 gctggaggta atttcctgct ttgtttttaa taaggaatac tgcgccagct aatcgtttat
    15781 ctggtttatt tgtctcgatt ttgacaaatt gcttactccc tgtatataca gagactttct
    15841 ttgtactgac accaaaacca aaatccagtg ttccgttgtt gttgatttcc ttgttcgcct
    15901 gtgctgtact gcttagctgc atctgataag aaatggtcag tttttttcca gcatattttt
    15961 ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg tttcttgtgt atgacaaagc
    16021 cttctgtcag cttttctcca ttcgatgaaa tcgttaaact attaggtaaa aacgttaatg
    16081 cttcatccgc actatctgac aattcgaact tttgatagtc aaggatattt acaggaattg
    16141 tcgtatgcaa ggaataagtg attttttcac caatcgtaaa atcatttcta ggctcttcta
    16201 ataccttctc aaaaggtgga tcagggtagt cgttttcttc atttttagga tacaaatgga
    16261 tagttgaaag tttttgattg ttttgtccat gtacaggaag aacaactacc atattctctg
    16321 ccttttcttt gacgacttct ggtactttgg attcaatgaa aagatacgct ttatctcttt
    16381 tttcttgatc tttagcggcc agagaaaaag aagcgatacc atcttcgttg ttaagagttg
    16441 tagttgttgc ttctgcggta aacataccgg aagacgcacc gttttttgcg atctctgctt
    16501 gtgcttcttc taccgttttg cccttttccc gtagatggta aaaagaatct gtgacatcat
    16561 aaacttggaa tgtgacacca tttaatcctc gatacgtttg taataaagct ttttcttgtc
    16621 cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa aagcaatttg tgtaaagtaa
    16681 tctgaaccat ttctgtttct gtttctgttg ctacctgagt aaaagctggt ttaaatagta
    16741 acaagaagag acacatacta agccaaccaa gttttttcat atttagctgc cccctctttc
    16801 ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca atcatcccta ctagatagat
    16861 tcctaaacgg ccaattcctc ccgtttcagg taatggtact tttgcctgat tcgtgatgtc
    16921 taaagaaatc tggttgtttt tggctcctgg tgacagaaca gattcatgat cttgtccatc
    16981 cacttgaatt gacccatctt cgtgtataac tatagtaact ggctctttta gaccttggta
    17041 tccttctggt gtaaaagttt cggtcagcgt ataagttcca ggttttaaat tttcgaatag
    17101 aaaggtatct gtttcttgtc catcttttgg cgattctaag tccatttctg gtccctgcag
    17161 tctgaatttt gctcctttta atgtctgtcc gttgtcttct tttttgtgta ccgttagatc
    17221 aaacgctttt agtcgatttt gatgagtgag cgtccataca ttatttgtca tctcggtatt
    17281 ttttcccgtg taaatgattt ttccatattt atcgatttgg aattcataat ttcccggcga
    17341 agtatcatat ccggcaggac cagctgtttc aacgactcga tatttaccag gtgtgagtga
    17401 atcaaaactg gcaagacctt tttcattcgt tgtttggctg tcaattggct gataagtacc
    17461 atttgcttct tttttctgta gggaaaatgc tgctccttta agatttatct ctttgtccgt
    17521 cccttgcatc gcatacttac gtactgctac aggaacttca acaaaaggat tttcaatagt
    17581 caactggatc gtatcatcgg aagtagtgac tgtttttcca tcaatggtta ccgtaccatc
    17641 agaagcgatc ttgatttccc aagtagtctt tttgcttaat ccatgccctt ctggagcttt
    17701 tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg acattttctg gaagagaata
    17761 ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa tctcctgtta atttgaaaac
    17821 agcgccaata agatcctttt cacctgtaga ggaatttttc gttattttca agtttaacgg
    17881 tgtgaattgt ttagtatttt tccatgtcat tgcatctatt tgttgagaat cgtatcctgg
    17941 tacaggtaat tctttgattg tttggtaact gaatgcttga ccttgattat tgtattgagg
    18001 tagtgataat atctcttgat aactttctcc gctatttgca gataatttgt caatgtctgc
    18061 acgttcccac gtatttgttg tatctttagc tggtttaatg attcgaatat atccgttttt
    18121 ccaagctgca gcatttgtcg tatgttcccg ttgaatctca aaagtaactt gatctggacg
    18181 atcagctaga ttgttgtcaa attcttccca taactttttg atgtgaagac tgactccggg
    18241 agctttagca gatggtatac cgaattcagc taattcattg gtatcaatgt ttggctggaa
    18301 agttgtcctg ccgttcattt gataccagaa atttggatgg aagtcctcat tttctgtttg
    18361 gattctcact tggtaatgga tttggatttc ttggtctttt cctaaataga tctgattgct
    18421 cttgatggta tttccttcta tggaaataga tggagtgacc gttgtaggac ttgtccccac
    18481 actcttgact gaaagtgtac caggctgata aatgaatggt tctgcgattg gatcatttat
    18541 ttgtccattg cttacagttg ctgagatctg tacagctttt ttggctaggt attcagagat
    18601 atctgttgca tgatcagctg attcatagta aagatcgcct ttttcatctg atgaaaccat
    18661 ttgtcgcata cgagactcta cttctgcttt tgagagacca gctgccggat cgctttgaag
    18721 ttggatgcca agaccatgta tttcgattcc tcgttctttg agtgccatcg cttctccgat
    18781 ggttgcgata aacgtactgt cgattcttct ggatagattg ttttggtcag gtgcatcata
    18841 gattcttgag attagagaag tatttcccgg ccgatcttgc gtattagaaa actgagttcc
    18901 gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact ttataggaaa atgttggtac
    18961 accatccgtc agcaaaacga tcactttttt atgtccattt ggaacggata gcatgcttcc
    19021 tgcatctctt agtgcttttt gtgtaaaagt accaccattt gtccgtgaag gtgtaatgga
    19081 ttttacttga tttttcactg aatcaaatga acccatctgt actgcaccgt tactgtagct
    19141 atatccttcg cttgagtagc cgacatatcc catattgatt ttgtctgtga taccgctatc
    19201 tgctaaagta tcgacaaaac gatcgacacc aatctttact tcaccgatcc gattattgtc
    19261 gttcatactt cctgaccagt ctacgaccaa tacgagatca agaggcgtga tatctttttg
    19321 tacatttcca cgagcattca aataaacatc aaacaatccg ggtgtacttg tttcctttgc
    19381 atactttcgt atcgcatagt ctgcttgatt accggttcct ccatattcga tatacgaatg
    19441 ggtccgatca ttaggatctc cattccaaga atttatacca tcccattgat ttgttccgtt
    19501 tttattgcct tgatgatcaa gaacatttgt atttcctgta ggttgccaac tggctttagg
    19561 ataagtgccc tgatcatctg ttgtatattc tgtaggaatc gtttgattag atggcacact
    19621 cacagctggc aaggataatg taggcaatgt aaatgggctg acagaatttt cgttttcttt
    19681 tgatgattct tcttctattt cgtctggctt tggtactgac acatctgtat ctttcttatt
    19741 ttctgtctca tcaataatgt catattcttt attatcgacg atcagcttgt ttgcctggct
    19801 gtggtaaagt gctggatcga ttggaaaact cagatcgata gtttgtttag tgtttgatga
    19861 gatttccaac gtaattttcc cgctatttac ctgataagca tagccatctc cttcttttct
    19921 ttccacatca cttaagtaat tttcgactag accaatttct ttttctatct tttcagcact
    19981 ggtgttattc gcatttagct tgatgttcag tgcttttttt ccagactgat ccgaaatgac
    20041 ttcagctgaa attgtttcta aaactgttgg atcatctgta gtgtctgcta ttgcagataa
    20101 tggcacaaag tttgataaac tcaatattat cagcatgaaa ataactttca gtttcttccc
    20161 tgtggtcgtc atttttcccc tcctttttct ttttttctgt tttgacgtga atagtgaaaa
    20221 ttattaaaat aaacaggcgt tacataattt tatttatttt aatatgataa ttattatacg
    20281 aagggcgcta aagggaatca caccaatttt cataccaatt ggtttcattt atgaagttga
    20341 ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt gagggagaaa aacatatgat
    20401 caggttgttg gaacgaaact atagaaagat ggaaaaaatc attcgaattc tgtccatttc
    20461 tgacaagtgg atgactaaaa aagaacttgc caaacgaatt ggtagttcag aatctacatt
    20521 tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg gatgtaacga ttcaatcttc
    20581 acaaaaattg gggtatcgtc ttgaacattt gaatgtacca gcttatttaa atgttctgat
    20641 ggaaatgtcg caaagttcta caaacagtcg tttattaaat gagattattg tcaatccggg
    20701 taaaacgatt gagtattatt gtgaaaaaat ttccatcagc cggtcttctt ttacacgtaa
    20761 gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta aaagtgatcg ttgaccaagg
    20821 ttttcagtta actagcctta aaagtgagct ccagttgcgt atctttgtta cctttttctt
    20881 tttaagttac tacggaagac gcacgttgcc atttccaatc gaaaaaaaga agatcaaaga
    20941 aattatggtc aagaataatt gtcagatgaa tttgtttcag gaagaaagcc catatgagca
    21001 atctttcttt attatgtatt ttgttgtgca tctcatgaga gaatcccaag gaactccatt
    21061 tgaatggaaa gctggagact cttatgtaca gttgataaat atcgataaaa aagacttctt
    21121 aatcttgaaa aatctcttag gaaaattgaa gtacagcagc tatataaaag tagtgaacta
    21181 ttttgtcaat atgatttttt cagaattttc ggagaaagaa aaaaatgaaa tcagtgagca
    21241 gatacgaaaa aatatgtcct actgttcgtt ttcatccgct gattgcaaag aaaaaaacga
    21301 agaatatgtt gttcaattat taacgcaaat ttattttttc tcccgtattt tcccatacga
    21361 tacgactcat ttgacgatcc gtgcaactta ctttgcacag cagatgcaac accgccagat
    21421 acgtctatat gctggatttc gtgcagaact tcaattttat tccactctac tgaatcagaa
    21481 tctgatttat tatttatcta gctatatctt ttggatgctt atcggtaatc ctgaaattca
    21541 ccattatact tctgataaaa aaatactgat tatcagcgac ttaagtcttc gacatagtca
    21601 atatattgaa gaatatatca gtgatattct tgctgtgcat aaaattcact ctgagacaac
    21661 tgctattact gaagatcagc tgtcgaaata caatcttctc gagtatgatc tgatcgttac
    21721 gaatcaaccc attttgaatg cccatgttcc tcatatattg attgatgata gtgtgtcatt
    21781 cgcaaatgaa gaagaactca tccgcttatt cgagttatga ataatcaaaa aacagctatg
    21841 tataaaccac cctgataaaa atttcaggac aaatacacat agctgtttct ttttattgta
    21901 ttccttcagc aatccaacga gatttatagc gaaagtcttt taacaactct tcttttgtcc
    21961 aattatttgc aatgagatcc gtcaaagtgt tcattacgag ggtcatggaa aagtatagga
    22021 aactaactgc ttcttccgac gattgaatgg agaaatgatt gattcttaat aattccagcc
    22081 atttttcgtt cattggagaa tcagccgata gttctctacg tttggttgtc tgctgatcat
    22141 ttcgctgggt taacaagtat aacctttgcc aatagatact ttcgtgatct aagtcgcagc
    22201 accactctaa atatttttcg atccccaaaa agaaatgttg tttgttttcg ctaatgaatt
    22261 ttaagatatc ttgatggact atgtgagaac tgcttttgat cgtatacaaa taagcatctt
    22321 ccaaatcttg aaaatattta taaaaagcac cgcgcgacat ccccatcttg tcaacgattt
    22381 ctgagacttt gacttggctg atgtgctgtg agcaaaatat ctccagtaat aggtcatcga
    22441 ttctctgttt ctttgcgttc gataaattga aaaaagtttc ttttggcatc cacatctctc
    22501 ctttatctct gattatacgt ggatagccct tcattgtcat gaaggattca ttccttcttt
    22561 ttctcttctt ctgtcaagta cccatcttcc attcgataaa cgcgatcact ccattgaatc
    22621 atacggacat catgagtaac cataatagtc gctttttgtt tctcatgagc ttctttaact
    22681 agtaatttga cgacttcata tgcatgatct gtatccaaac tagctgttgg ttcgtctgct
    22741 aagatcaagc ttggctcatt atataaagcg cgagcaattg ccactctctg tctttctcca
    22801 ccagaaaggt ctttaggata actattttta agtgactgga tatctaaaga ttgcagcaag
    22861 gaatcgattt ttgttgaatc ttcttttttt gaagtaactt ggtcaactag ttcaaattgt
    22921 tcctttaccg ttaaaaaagg aatcaaatta gagctttgga gaataaatcc tatttcttta
    22981 aagcgtaaat ctgctcgttt tttttctgga agctcagtta gtgagtgacc gttgatcact
    23041 atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg taaggaaagt acttttacct
    23101 gaaccagaag gaccaatgat gctgacgaat tctccttcct ttacataaaa atcaattgct
    23161 ttcaaagctg agacctttgt atgaccagaa ccaaatgttt tggtaacttt tttcatcgtt
    23221 aatatttttt tcatttttat taacctccta ttgctgtaat tgggtctact ttggttatcg
    23281 tacgtatcga aaacaatccg cctagcattg cgacaatgat caaaatccca ctatatagca
    23341 accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg taaaatcaga ctagtcaaat
    23401 aagcaaacaa aagagcaagc agaacgccta ccaccccaac aataaaggac tgtgctacta
    23461 aagatttagc gatgaagaaa tttttgatcc cttgtgcttt cattactcca aaaatagcag
    23521 tcttctgaag tgtcatcacg tacatgaaaa taccgaccac ggcagcaacc actaagaaca
    23581 aaaagtatat catcgcattt aaggtcatat tttgggcaga atagccagga atactttcaa
    23641 tgaatgtccc aatagttaat aactgtagac ccttcgatgc tttttctttc gaaattttag
    23701 ctgaatcctt tattacgatc ccattgatcg gctgttcttc ttcagaagca aacggctgtt
    23761 gtccatactt caaagcagtc cacgtatcta gggacgcata gatgacggga gaaactgtgt
    23821 aataagtttc aggaaagata ccgacaatcg tcaaaggatc gtcgtaactg ccaattttga
    23881 tcgtatctcc aattttatag cctgaatcag ccagattttg agagatgatg atttcattct
    23941 ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa aaatgcttct ttcgttgtgc
    24001 cgaaaactgt aacattttct ttgttttttc ctttgattgc tccgctgtac aatccgattg
    24061 gtgctttctc tcctccttca atgtatttca gatctcctct tgttaattgt gaagctgcaa
    24121 atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc gtcccaatca tcgattgctt
    24181 ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa catgaatacg acatacgcaa
    24241 tcaagaacat aataccgacg atcaagccat atcgtaattt agaatagcga atttctttga
    24301 tagctagaaa catttgtttt tcctccctat gatagattga tagtgacaaa atgtcacttg
    24361 ttgaatagtg taactctaaa gtgacaattt gtcacttgtg ttgttttttt cttatcaata
    24421 aattgtctaa gacatctttc gataaaacct taacatgagt cataaaaaat ttatttaagc
    24481 cttatttatg ctttccatga taaactaaag tgaccaaatc aatgaggtgg gaaaatgatt
    24541 aaactattag tagtagaaga cgataaagta ttatccaata atataaaaga aatactcgct
    24601 gcaatcggcg agataacaca agtttatgat ggtagtgaag gactgttcga gttggaaagt
    24661 ggagtttatg atcttgcaat cttggatttg atgatgccgg aaatgaatgg atatgaagtg
    24721 ttagcaaaaa tacggaaaga aaaaatacag actccagtat tgattttgac tgcaaaagat
    24781 agtttggaag ataagattga aggatttgaa cgaggtgcag atgattatct aacaaaacct
    24841 ttctatcgag aagaactgat catgcgcgtg aaagcattat tgaaacgctc gctaggtctc
    24901 tttgatgaaa atgttctgaa gtataaggat atcagttgtg atttaaaaag caatgaagtc
    24961 ttgtataaag aacaagtttt gcccatacaa ggaaaagagt ttgaattgct cgtttatttt
    25021 ttacaaaata agggaatcat tttgacaaaa gagcagatat ttgaccggat ctggggattt
    25081 gattctgata cgacgattac agttattgaa gtttatatga gcaacttacg aaaacatctg
    25141 cgaagtactg gattggataa agaaatcaaa accttacgta atgtcggtta tatactgcaa
    25201 agggtgtgat gcaatgaatg gagatattac acgaaagcaa cgtagtcgct tttttgcgac
    25261 aaatgtagca gcatttgctt ttatcttttt tcttttggga ttcattactt tacagatatt
    25321 aaaccaatct gcatacagag aaacagatct gtcattagag aaaataaatt ttgattctcg
    25381 aacagtacaa ttagagatac aaagatatca gcaaggcgat ccctttttaa acccgactca
    25441 aacggaacaa aacagaacaa acatggaaca gccattaatg gaaccatcaa atgaccgttt
    25501 taacactcaa gtgatcttgt ggtctaaaaa tggtgagata ttgaataaag cagctattgg
    25561 tgggcgtttc aaccaaatca aagaattaaa aatggatacg aaaaatctga ataccatcca
    25621 aaatattgaa ttaaatgaag acagtggctc atctgccctc tctttccgtt cgattacgaa
    25681 aaaagcggag acgaatcaag atggcgttgc ctacatccaa attttagaaa atacaaatca
    25741 aatcaagaat tcattagcga cttttcgcac gattatgatt ttgtgtatga ttgttttttg
    25801 gattctctct attggtatca gttattactt gtctagtcta agcatgcgcc ctattattct
    25861 ttcttggaga agacaaaaag aatttgttga gaatgcttct catgaattgc gcacaccgtt
    25921 aacgattatt caaaatagtt tggaacatct ttttaccaag cctaatcata ctattattga
    25981 agaatccgaa agcatcgctc aagcattgtc agagacgagg cgcctcactg gattgacttc
    26041 ggatttgctg acaatcgctc ggaatgattc tgatcagcag ctgctttcaa agcaaatgat
    26101 caatacacaa gaatatatcg aacaacttgt taaaccattt caagagatgg ccatcataga
    26161 tggaaaagag tttattctcc ataacgatgc aacattagaa gtaccaattg atgaaaaaaa
    26221 aatccatcaa gttttggtta ttttgctgga taatgcatta aaatatacga atccagaaga
    26281 taagatcatt ttggattcat ctgttaacca taaagaatgg ctgattcacg taaagaatac
    26341 tggcccttct atcagtgaag aagatcaagc aaggattttt gatcgatttt atcgggaaga
    26401 tcaatctcgg gcgaaagaga ctggcggata cgggcttggc ttggcaattg caaaacaggt
    26461 aatcgaacag catcatggac agatttctgt atccaattat cttccacaag gagtcgattt
    26521 taaagtccaa ttgccgttga agcctaaaaa ataatgctta tccctaaaaa agggctgtga
    26581 caaaaatctt gtcacagccc ttttttgttg cga
//
LOCUS       DACEPW010000017        26363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEPW010000017 DACEPW010000000
VERSION     DACEPW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.017"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(73..1965)
                     /locus_tag="IW819_000556"
     CDS             complement(73..1965)
                     /locus_tag="IW819_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1243685.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1962..3689)
                     /locus_tag="IW819_000557"
     CDS             complement(1962..3689)
                     /locus_tag="IW819_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1243686.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3705..4355)
                     /locus_tag="IW819_000558"
     CDS             complement(3705..4355)
                     /locus_tag="IW819_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1243687.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4559..4855
                     /locus_tag="IW819_000559"
     CDS             4559..4855
                     /locus_tag="IW819_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1243688.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5027..5647
                     /locus_tag="IW819_000560"
     CDS             5027..5647
                     /locus_tag="IW819_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1243689.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5739..6905)
                     /locus_tag="IW819_000561"
     CDS             complement(5739..6905)
                     /locus_tag="IW819_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1243690.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6917..7270)
                     /locus_tag="IW819_000562"
     CDS             complement(6917..7270)
                     /locus_tag="IW819_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1243691.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7398..8420
                     /locus_tag="IW819_000563"
     CDS             7398..8420
                     /locus_tag="IW819_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1243692.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8859..9293)
                     /locus_tag="IW819_000564"
     CDS             complement(8859..9293)
                     /locus_tag="IW819_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243693.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9403..11262)
                     /locus_tag="IW819_000565"
     CDS             complement(9403..11262)
                     /locus_tag="IW819_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1243694.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11380..12540)
                     /locus_tag="IW819_000566"
     CDS             complement(11380..12540)
                     /locus_tag="IW819_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ1243695.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12847..14271)
                     /gene="araA"
                     /locus_tag="IW819_000567"
     CDS             complement(12847..14271)
                     /gene="araA"
                     /locus_tag="IW819_000567"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1243696.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14287..14985)
                     /locus_tag="IW819_000568"
     CDS             complement(14287..14985)
                     /locus_tag="IW819_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1243697.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14942..16603)
                     /locus_tag="IW819_000569"
     CDS             complement(14942..16603)
                     /locus_tag="IW819_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1243698.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16821..18221
                     /locus_tag="IW819_000570"
     CDS             16821..18221
                     /locus_tag="IW819_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1243699.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18473..19552)
                     /locus_tag="IW819_000571"
     CDS             complement(18473..19552)
                     /locus_tag="IW819_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1243700.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19638..20597)
                     /locus_tag="IW819_000572"
     CDS             complement(19638..20597)
                     /locus_tag="IW819_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1243701.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20609..22132)
                     /locus_tag="IW819_000573"
     CDS             complement(20609..22132)
                     /locus_tag="IW819_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1243702.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22142..22705)
                     /locus_tag="IW819_000574"
     CDS             complement(22142..22705)
                     /locus_tag="IW819_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1243703.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22711..23574)
                     /locus_tag="IW819_000575"
     CDS             complement(22711..23574)
                     /locus_tag="IW819_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1243704.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23588..24484)
                     /locus_tag="IW819_000576"
     CDS             complement(23588..24484)
                     /locus_tag="IW819_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1243705.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPGICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24659..25930)
                     /locus_tag="IW819_000577"
     CDS             complement(24659..25930)
                     /locus_tag="IW819_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1243706.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(25920..>26363)
                     /locus_tag="IW819_000578"
     CDS             complement(25920..>26363)
                     /locus_tag="IW819_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1243707.1"
                     /translation="ETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSANYATEDFEEV
                     PYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSGFDTKATNLPK
                     SEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         7689 a         5536 c         4573 g         8565 t
ORIGIN      
        1 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
       61 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      121 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      181 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      241 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      301 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      361 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      421 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      481 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      541 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      601 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      661 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      721 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
      781 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
      841 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
      901 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
      961 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1021 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1081 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1141 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1201 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1261 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1321 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1381 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1441 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1501 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1561 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1621 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1681 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1741 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     1801 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     1861 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     1921 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     1981 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2041 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2101 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2161 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2221 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2281 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2341 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2401 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2461 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2521 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2581 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2641 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2701 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     2761 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     2821 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     2881 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     2941 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3001 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3061 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3121 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3181 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3241 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3301 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3361 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3421 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3481 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3541 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3601 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3661 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3721 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     3781 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     3841 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     3901 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     3961 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4021 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4081 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4141 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4201 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4261 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4321 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga tcctcctttc tttcaagatg
     4381 acacattgtc atatgacgct gtgtcattat aacacctcct acctgttttg caagacattt
     4441 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4501 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4561 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4621 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4681 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4741 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     4801 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     4861 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     4921 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     4981 tctcatagtt ataacaatca tagcaataaa aggagaaggt aagcatatgg catctgtttt
     5041 agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc acagttaagg cattgacgtc
     5101 ttttttaaca agttataaag aaaatcatcc agatgatgaa gtgactgttt tggaacttta
     5161 tcgcgatgat attccggaaa tcgatgaaga attattatca ggttgggaag cattacgtgc
     5221 tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa aaaattgctc gtttcaatga
     5281 attgactgat cagttccttg ctgctgataa aatcgttatc gcaaacgcct tatggaatct
     5341 aaatatccca acaaaattaa aagcttggtt cgataccgtc aatgtagctg gaaaaacgtt
     5401 ccgctataca gaaaacgggc cagaaggatt agtgacaggt aaaaaagcac tgcacatcca
     5461 atctaatggc ggtgtataca acggacaaga ctttgcctct caatatgtaa aaggtatttt
     5521 aaactttgta ggcatcgatc aagtagatca gctattcatc gaaggaatcg attatgaccc
     5581 tgaccgtgca gatgagttga tgcaaaatgc attagataaa gctgctgctt taggtaaatc
     5641 attttaaaaa ttgagataaa tgcatacaaa agagacagag gaaaaaatct tcaaagcagc
     5701 atttgtacct tggatatttt cccatctgtc tctttttgtt attctttctg ctttttcaac
     5761 ggattttctt cataaagaga agagattgtt gtgtaccatt caaaaacatg agtaatcact
     5821 actttagctg ctgcataaac aggaatcccc aagatcacac cgatgattcc gaacaatttc
     5881 ccagaagtca gcagaactaa taaaatcgtt actggatgga ttgctaactg attccctagt
     5941 actaatggag agatcaagcg tccttctatt gtctgctcta ctgcaaaaac gatcagtact
     6001 ttgatgatca tgaatggacc tgctacaatg gctaaaaaga tagccggaat catcgctaaa
     6061 aatgatccta agtaagggat caggttcaaa aaacccgcaa taatccccaa cgtgacagca
     6121 tactctaaac caatgaccgc aaaaccaatc ataaacatga ttgccacagc aaaagccaca
     6181 gttagttgtc ctcttatata agaagaaact tgactattca tttcactcag cacattcaac
     6241 gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat aaggtgctaa ttgtttaccg
     6301 tctttcaata gataaaataa gataaacggc atcgtcagca gagccactaa aatagtcgct
     6361 accgcgccga caaaacttcc aagactctga actgtactct tagatatatc ttggatcata
     6421 tcgcctgccg aattcatgaa cttatcacct gctgtttcca actgttcgcg aaattggttg
     6481 aacagcgggt cccgcaaaat ctcggttaat ttattatcaa tcgtatctac atattgaggg
     6541 aagttactga tgaagctcac tgtttgttcc tgaatcttag ggatgatcac tacgctcccc
     6601 catgcgatta gtgcaacaac caaaacaaac agcccaatga tactgtaaag ccttggtact
     6661 tttcgctttt caaagtaatc aactaccgga ttcatcaagt aatacaagat tcctgctaaa
     6721 atgataggca gacctacgat tgctaaaaat tgccagattg gtgcaaacaa atatgaaact
     6781 tttgtaaata aaaagacgat cagcaatatt aacaatacaa caagtaaggc tgtgactact
     6841 tgattattta aaaaccagcg ccaaaaccac gataccggtc tgttcttctt attttttcct
     6901 tccataattc ctcctgttag ttataaaaaa tttggcttcc caaaccaata gcaggcagtc
     6961 tatgcggatg aagatagatt ccatttgcaa tttgtccttc ttctggacaa gcatcaaaaa
     7021 tcaatgatac gtgagctact gtctctaaat tcccattggc catcggaccg acataatcga
     7081 tcgtatatac ctgatcatca aaagcaattg ttccgcctgc tttcaaagaa aaaggttcgt
     7141 cttttgatac ttcttgaatc accgaatatt ttcgtaaggc agctgttgct gttttatcga
     7201 acaaaatcag catgggttct tccttgttta ttgcttctgt cccaatttct gtaatctttg
     7261 cctgaatcat tgcactctct cctttaattg ctttcaaaaa cagtataaca cattctcatt
     7321 ctttagccgt gtggaataat tatgtgatag aataaaaacg aaaactattt cattacaatt
     7381 aggaggaatc agctcatatg atcgaaaaga taattttagg aacctatacg cgtcgcgaaa
     7441 gtaaaggaat ctatacgatc gaccttgata cagaaaaaga agaattagcc aacctttcat
     7501 tagctgttga agaaaatagc cctacctatg tagcaaaaag taaagctggc aatctttaca
     7561 cagttactag tgtggatgga ttaggtggtg ccggtgcgta cgataaggat tttcattttt
     7621 tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt agcggttgat gaagcacgtc
     7681 aattactata cggggcgaac tatcataaag gtgaagtaaa tgtctaccat atattaggag
     7741 atggcgaact gaaagcagca gattcaatat tccatcaaga agcgacaggt cctcataaaa
     7801 accaagatca tgcgcatgtc cactatacgg atctgactcc tgaccaacga ttagtcgtat
     7861 gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc cgaaaacggc aaattgaatc
     7921 ttgtaactac cttcactgca gagccaggaa caggccctcg ccatcttgtc tttcatccaa
     7981 atggacaatt cgcttattta tttggtgaat tagacagcac agtccgcgtg ctttcttata
     8041 ataaagaaac aggtgctttt gaagaattac agaaaatatc gaccttgcca gaagaattcg
     8101 acggtgaaaa tggaggggca gctatccgca tttcaaatga tggcaagttc ctttatgctt
     8161 ctaaccgagg acataactcg attgctgtat tcgcagtttt tgaagatggt tcccacattc
     8221 aaaacaagca aatcatctca acagaagggg attttccccg cgatttcgca ctagatccaa
     8281 gcaataattt tgtcgtgtgt gccaatcaga acaccgataa tctgacatta tatcgccgtc
     8341 atcctgaaac aggattactg gaattactga aaaaagatgt ctatgtacca gaatgtgtct
     8401 gtgtttattt tgatcgttag taagaaaagg agaatgaaat gaatcagcaa gacgtattag
     8461 acagactacg ggaagaacta aatattcctt tctttgatgg gaaaatcgaa gataaagagt
     8521 atagcgaaga ggaatatcag aaactcaaag ccgatttaca gaactacttt gaacaatatg
     8581 tacagaatgt agaaaactaa tcagctttcc atactatctg aaaagaacat caggacaaga
     8641 cttttccgtc tatcctgatg ttcttttcta caaataaaaa accgccgaga tggttagact
     8701 cgacggaaaa ggagtattta ctcgattttg agtaagtaca aggttgttgt tggttatagg
     8761 tatgtcatta tcataaagga ttttgatcac tctatcataa aggtaatatt acatttatat
     8821 tagataactc tagtttttat tgttatcaaa gatttctttt attttttcaa caacaaatta
     8881 gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt ggtcttgttt tgcttcagat
     8941 gtattgaata gatcttcatc tacccctgat tcaagatcga tactttggat catttcttca
     9001 cagcctgcta catatgaaag ataagctttt tcaaaatttt tatgaatgcc cattgctttt
     9061 gctggtggac gcaaagtact gactttctta agcattgccc gatatttttt tgttccttct
     9121 tcaaataaca ggatgatctc ttcaagagtc gatttgtcca gttcacttgt ttttccttca
     9181 tcaattgcgt ttctgatcac ttcatatttc ggatgcattc cttctccgat tgcttctgtt
     9241 tccttgacga tttgatcgat ggactgcaca taatagccaa tattcggtaa catgttcgtt
     9301 ttcctacttt ctccattgtt gttattattt attctacttg tttttatcct caaatgacaa
     9361 gtacttgaaa aaatttaatg gtttctatac agctcgccat tcttattcgt gctcaaagaa
     9421 accatctgct aaactatatt gtttccctgc gacaatgtca tattggatca attgatattc
     9481 gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct ttataccact gtccgttttg
     9541 atagtacagt tctcgttcaa acgccggaag ttcctgttct aatgataaaa gcagctgata
     9601 aaagccattt gttgtctgat tcgtctgttc cattagattc gctgcaaaat aaaacggact
     9661 ggtgattgca tcgtgagtcg tttgcttttc taattctcct ttactgtcga acataagaaa
     9721 ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg atcgtatctg aatagatccc
     9781 tggaagatga tccccccaaa aaacaactaa agtccttcta gaaagattct tcaattcttc
     9841 cgtgaactgc ttcagtgccg tgctgctata ggaaatatct tgcagataat tctctaagtc
     9901 aagtgtccca cttccatctg aagtttttgc aggatagtct agattatcat actttccatc
     9961 gtagggcata tgtgtctgca tcgtgactaa atgaatgaac tgaggtgttt tttcttcctt
    10021 gagaagtgtc attacttctt tgtaagctga ctcatcagaa atatatggat tattctctat
    10081 cgtatccgta taggtcatcg tacgctcgct gatgaactgg tcaaatccta atgtctgata
    10141 aacatcctct cttttataca tcgaagtatt gtatggatgg atcgctgtcg tctggtatga
    10201 ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc atttttggta caagcatcgt
    10261 gtaaggggtg gtgagttgcg cattaaataa cgccatagaa aaaccagtca atgcttcaaa
    10321 ttcgatattt gctgttccac cgccatagtt ttgagaaagc atattgccac tatatgtttg
    10381 atcagctact tcataataat ctgccaatgg ctcaccagat acttctacgc cgtttaatcg
    10441 actaggatct gagaaacttt cactcatcac gaacacgatg ttcggcgact cttcctctac
    10501 agttttattt ttttcatcgg ctagcttctg gtatttttca gtgatttctt tgatcttttc
    10561 tttcgaatat ccttcaggtt catccattgg ttctaccttc agattgtaca aaaacccacc
    10621 gataaaacca gtgttatagt agttcatttt ctggctgtaa ggtatccata gtgccgtttt
    10681 attatatgct tttcgcaaca aattatcggg attgttgaaa ttactgatat accctagcag
    10741 tccaattgcc acaagcagtg tcgttaatcg gatcaattgg aatcgccttc cttttttcag
    10801 gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt cctgcggcag ctaagatcac
    10861 tgtaaacagc atagtgccgg tcatatcttt cagcaatccg atttcagtga tcatcttcaa
    10921 atcgtctgga taaatcggtt cttgccgata actcattttc atataatttg caaaacctaa
    10981 tacaccgatg gctacgacat acgtaaggga acctaaaggg acagatcctg ctagtgcaat
    11041 cagaaaaata agaataatca gcaagacgag acaggctaag aagaattttt ccgtatgcca
    11101 agaaaaagca aatttcaacg caaggtctac ggatagatca ttttgacacc actgcaaaaa
    11161 taaattacta aaaacaacaa gaaaaaagat actgatgatt gctgctgttc cttttaaaat
    11221 ctttttctgt ttggtcgaaa gtctgcttat atatcttttc aaatgcttgc tccttcattt
    11281 ctatcaataa aagaatgttg cttctcttat tttagttgta cgaaattcga aagtaaagaa
    11341 cggaaagaag aattaatgaa atccaaatat tctctttctt tatggccttt ctggtttctt
    11401 taataagcgg acctttcgta ctccagaaat tttttctagc tgttgtttca cttgattgag
    11461 ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag acatgctcat tcttatggtt
    11521 acgcgatata tctgcgatcg ctagctcgtt ctcattcagc agagaaaata tctctgctaa
    11581 tatattggtt ttcttttgat aaaatatagt gaatcgaaac ggcgcttgga aaaacattcg
    11641 tgcggatgga taattgaccg cttctctaac cgtaccgaac aaaagataat cttttaaggc
    11701 ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga gtcgatcccc ctagatgagg
    11761 caacataagt atacggtcat tttctaagaa ctcttcttct ggaaaatcag tcaagtattt
    11821 agccagatgt tgtttttcca atgcttgcat aacagcatat ttatcaacaa tttcacttct
    11881 tcctacattg ataagtacag catctttttt cattttttct atattcgcct gatcgatcaa
    11941 tccttttgta tcttctgtca acggcagcat gactacgatg taatccgagg tagaaagaac
    12001 tgtttctaaa tctgcttgcc gaacataatc caactgggca tcgcggatag aataacccaa
    12061 gacatccatc cctaagctat agcaacttag cgctactttt gtgcctatcg ctccaagacc
    12121 aagaagaccg atggttttgc cttgcaattc tcgtcccaca taagcacttc ttttgttttc
    12181 tgcctgttct aaaatattag gacctgttag tgtttgtacc atacgacttg cttcgattat
    12241 cggacgggaa gataagagca aacagcacaa aacaagctct tttactgcat tagcatttac
    12301 acctggtgta ttcatgacga tcgtcccgtt ttcagaagct ttttcaacgt tgatcgtgtt
    12361 caccccaaca ccagctcttg aaattgctag caaatctggt gtaagccatt catctttcac
    12421 tttcgtacta cgaataacta ttccttcggc tggcaaatca tctattattt tgaaatattc
    12481 cccttgtaac ttttctatct cttgttctgg aatagcatca atcaaatgaa cgtgatacat
    12541 aaaaccccct ccttactaac aagtatctcg aaatttctgc aattagacaa ctgtttacca
    12601 tttgacttcc tagaagcaaa aagagcagtt agagcagagt aggctgagtg ataaaaataa
    12661 ggctatgaca agttactgcc ataagcctta ttttcgagag caaaatattg agggtgagat
    12721 atcttgataa atggtgttcc gttagctgac attgataata agactaaatt tcaaaaaagt
    12781 tgaaaagcaa atttcgtcaa tctcttctta tttcttaaaa ctaagtggct ttttcacaac
    12841 ctctttttat ctaataacca ctgtttccaa ctcaactaat tttgcccacg tttcaatttg
    12901 atctgttgtt agatttagcg agacaacggt atgatgaccg ccacctgcct gaatccatga
    12961 atggacacct tcatggaaat tcggtttagt tttccataac acacgtgcaa caggtaaatt
    13021 aggtgcttca gtcgttggtt caaaggcttc tacttcattg atcagcagtt tataatgcgt
    13081 accgaaatct gccatcgata cgactacccc ttctcctgct ttcccatcaa atacaagacg
    13141 cgccggatct tctctatctc ccattgataa aggagaaaca acgattcttg gtttcgtcgc
    13201 agcaagcgtc ggatctactt ccatcatatg ggattccaag attgcttctt ggccactagc
    13261 tagctcgtaa gtataatctt ccatgaatcc tgtatctttc cctcgagcca tgattttcag
    13321 caaccgatca atcgctgcag ttttccagtc gccttcccca gcaaaaccgt atccttctgc
    13381 catcaaacgt tgtacagcta gtccaggtag ttgtttcatt ccatacaggt cttcaaaatt
    13441 agtcgtaaaa gcgttatagc ctccttcttc taagaaacgg cgaataccga tttcctgctg
    13501 agcttgtact tttacatgtt cctcccatgt cttttcttca taatcaccat agtcgaaggt
    13561 atacaattcc ttgtactctt caaataattg atcgacttct tcgtctgata cgcgatcgat
    13621 tacttgtaca agatctccga taccaaaata atcaacagtc caaccaaatt gaatctgtgc
    13681 ttccactttg tctccttctg tgacacccac gtttctcatg ttgtcaccaa aacgagccac
    13741 ttttattcca aaactttcat tataggctac tgcaacatcc atccaatcag caatctcttt
    13801 ttggatctca ggtcgtttcc agtagccaac gacgatttta ttttgtttat ttaatcgagc
    13861 attgatgaac ccatattcgc ggtcaccatg agcggattga tttaaattca tgaaatccat
    13921 atcgatcgtt ttccaaggaa tgctttcgtt atattgcgta gctaaatgaa gcaatggttt
    13981 ttgtagaagc tttgtcccgc ggatccacat ttttgctggt gagaaagtat gcatccaagt
    14041 gatcacacct gctacttctt ctcgataatt gacctctttc atgatttttg tgatcgtatc
    14101 agcagtaact gctaattctt gaaggcggat tggataaggc aattggccat tttcattcag
    14161 ttcatcgacc atttcttgtg catgtttctt tacttctcgc agtgcttctt ccccatataa
    14221 atgctgtgat cctacaacaa accaaaattc tttttctcca atattcaaca taattagcca
    14281 ctccttttat ttgttttgtc cgtaatacgc attttttccg tgttttcgta aataatgttt
    14341 atctaaaatc cgttgaggca gctcttctga aaatgaattg agctgtctgg taaataaatt
    14401 catcttgcat acttcatcta gtacgactgc gttcattacc gcactttgcg catctttccc
    14461 ccaagtaaat ggaccgtgtc cgtgtaaaag tacccctgga attgctaagg gatcaatttt
    14521 gcgttctttg aatgtttcta tgataacttt tcctgtttca tattcgtacc cgctgtcgat
    14581 ttcctgttgt gtcaaaaatc gagcacaagg taccgatcca taaaaagtat ctgcatgcgt
    14641 cgtccccatc gcaggaacat ccaatcctgc ttgtgcccag atcgtcgccc atgtcgagtg
    14701 agtatggacg attcctccga tttgaggaaa tgaacgatat aaaacagcat gagtaggcgt
    14761 atccgaagag ggattgagtt taccttcaat gatatgattg tccaagtcca caacgaccat
    14821 gtcgtctgct gtcatctctt cataactgac tccgctaggt ttgataacga aatatcgtgt
    14881 ttcaggatca aaagcgctca catttcccca tgtatatttg acgagtccgt gtttaggcaa
    14941 gtcaagattt gcttggtata cttcttcttt cagttgttcc agcattttct atcactcctt
    15001 tttcttgcaa ttgatgaatc gctgtttttt caatgtcgat cccctcacgg taacgtctca
    15061 taaatatctg atagcctgct atttcttctg ctgaaggtgt gatggtgatt ccttggtctt
    15121 gtccaaacac gtcttctgcc aagaagtttt ctaaagactt cccatgtcga tccatcatat
    15181 aggctgctaa taacgcgatt ccccacgctc caccttctcc ggctgtgtcc atcactgtaa
    15241 ctggtgcctc catagcagat gctagaatcc gctgtccaac ttctggtgtt ttaaaaatcc
    15301 ccccatgccc cactaaacga tcaatttgga cattctcaga tttcaatata tccattccga
    15361 ttctcattgc accaaaagca ctggctaaat ggatccgcat gaaattcgct aaatcaaaac
    15421 ggctacctgg tgtacgtaca aataatggcc ttccttcatt tactcctgta atgttttcac
    15481 cggaaaaata accataagaa agtaatcccc cgcaatccgt atcgccttct agcgctttgt
    15541 tgaataatac cgtgaataat ttatctctgc ttatctccat acccaaagaa ttggtaaatt
    15601 cttcaaaaat actgacccaa gcattgatat ccgaagagca gttattcgta tgaaccatgc
    15661 ctactagttc tcctgaaggt gtagtgacca tgtcgatttc cggatggaca ttttctagtt
    15721 ctttttctaa tacgatcata gcgaaggcgg aagtaccagc cgaaatgttc cctgtccgtt
    15781 tggctacact gttcgtagcg accataccgg ttcctgcatc cccttctggc ggacaaaccg
    15841 ggattcctgg tctcaaattt cccgtagggt cgatcagata agcccctgat tcgcttaact
    15901 tgccagcttc ttctcctgct actagaactt taggcaaaat gtgatccaag tcaatcgcta
    15961 cgtgcgcttt ttcgattaac tgaccaaaaa tatttttcat ttcttgatgg taggttttcg
    16021 ttgaaggatc gataggaaac atgcctgatg catctcctac gcctagtact ttctcaccag
    16081 taagctgcca atggacataa cctgcaagcg ttgtcaaaaa attaatttct ttcacatgtt
    16141 cttctttatt caaaatagat tgatacaaat gagcgatact ccatctctgc ggaatattgt
    16201 attgaaattt ttccgttaat attcgttctg cttcttcagt gattgtattt cgccaagtac
    16261 gaaatggtac tagtaactgt tccttgctat caaatgctag ataaccgtgc atcattgcac
    16321 tgaacccaat cgacccgatt gtcgtaagat tctcgccata gatttcgaat acttctgctg
    16381 ccatttttcg gtaactgacg cgcagtcctt tccaaatatc ttctaatgaa taggtccaga
    16441 taccattttc tagtttgttt tcccactcat aactaccagc ggcaatagga gtaaaagatg
    16501 aatcaatcaa tacagctttg atcctagttg atccaaactc aatccctaaa gatgtacgac
    16561 catctatgac atctaacata ctttgcgcat ttacttcctt cataatgatt taactccctc
    16621 cttctcgtca aacctgatca aaccgtcttt ttagtagcgc ttacaatgat tattgtatac
    16681 ttttgtacgt acatgtcaag cggttattta caagaataca ataaaaaaca cttgttttgt
    16741 acgtacataa gttataaaat gaaattgctt acaaaatctg atcaaccgcc ttctcgaaat
    16801 tctagaagga gagaatactt atgaaaaaag aaaaaaaagt ttcctcatcg tttatttatt
    16861 tttttggcgc atttggcggc attctatttg gatatgatat tggtgttatg accggcgcct
    16921 tacctttttt acagcatgat tggggtttgg caggtaaagc ttctttgata ggatggatca
    16981 cgtcttcggt catgttaggt gcgatccttg gcggctcttt atctggtctt ctttctgata
    17041 agcttggacg acgaaaaatg attcttcttt ctgcacttat ttttatggct ggttctgtcc
    17101 tttctgcatc tgcccctcac aatggttctt attttctgat tgcagcaagg attcttctag
    17161 gtttagctgt tggcgctgca tctgccctag ttcctgctta tatgtctgaa atggctcctg
    17221 ctagactaag gggaagattg tcagggatca atcaagtgat gattgcttct ggcatgctcc
    17281 tttcctatgt tgcagattat ttgctaaaag tgttgcctga aacgatggct tggcgcgtga
    17341 tgttagggct cgctgctgtc cccgcgctga ttttattttt cggtgtttta gctctaccag
    17401 agtcaccgcg ttttcttatg caatctggcc gcttggaaga ggcaaaaaga gtattgaact
    17461 atattcggac accaaaagaa gctgaacagg aattcgaaca aatccagctg aatgtcaaac
    17521 aagaaaagac aaccggaact tcttggcata ctttgttttt agaaaaatat cgttccttag
    17581 tcattgccgg tattggtgta gcagtattcc agcaatttca aggagctaat gctatttttt
    17641 actatattcc gctgatcgta gaaaaagcga ctgggcatgc agcgagcgat gcattgatgt
    17701 ggccgatcat ccaaggaatc attttagttg ctggttcttt gctctttttg gttattgcag
    17761 acaagttcaa tcggcgaact ttattgaaaa taggcggtag cgtgatggga ctttcattca
    17821 tcctaccagc tgttttagga actgtactgg atgctcatac aaattcgttg ttgattctct
    17881 tgtttttatg tatttatgtc gcattttatt cttgcacatg ggctccactg acatgggtaa
    17941 tcataggcga aatattccca ctagctgtcc gcgggcgagc atctggccta gcctcttctt
    18001 ttaattggat tggttctttc ctagttggct tgctttttcc tgtgatgact gcttctatgt
    18061 ctcaagaaat cgttttcggc atttttggga tcatctgctt tttaggggtt ttatttattc
    18121 aagagatcgt accagaaact cgtgggaaat ctttagagga aatcgaacaa agtgcatcta
    18181 aaaaaactta tcctaaacgt atttctatcc atcattctta aagaggttgt gacagaagtg
    18241 gtcagcttca agaaataaga aggaaccgct tagtttccag tcacaaggaa caatttcaaa
    18301 aaacagcttc tcatgtttga tgaatattgt gacttgtgac cagttggtag gagaacaccg
    18361 tttagtggag aggtggtgaa agaaacgttc agccttgagc gatgacaaaa gctctcacaa
    18421 acattgcgac gagtaaccgt aggagcaata agaggcggta aaaacccgtc tcttattttt
    18481 cgttttcttt cactgtttcc cgttcaacta gtacaggttc atagagaata tttggctgtg
    18541 cctgtccttt ttcaatcgtt gtgacgatcc accgtgcggc atcttgtccc aacttttctt
    18601 ttggatgcgt caatgttgtc aattttttat tccctaaatg gcttaaagaa gaatcgtctg
    18661 ttcctacgat cgataggtca tctggaatat gatagccatt ttctgtcaac ttatctagca
    18721 ttaaactagc tatttgatcg ttatagcaaa tgatgccatc taaagcttgt tcttctttca
    18781 accgttgtac tgttttttca gcgattgttt ctcgtgtttc cgtggtgaaa gtaatgatat
    18841 cttccgtcaa aaaacgcaca ttatactgtt cacacgcgct gataaaacct ttcatcctgt
    18901 attttccttg aagatcgtcg atttttgtga tgaacaacag acgctgatgt ccttttttta
    18961 tcaaatattc tgctgccata aaaccgactt tcacatcatc taaacaaata taagaggcat
    19021 ttaactcttc ataagcagca ttgatcatca ccatcggaat gccttgttct ctcaaagcaa
    19081 cataaagtgc taagtttggg ttatactgat tacttttcgt aggctcaacg atcaaaccat
    19141 ctaccccata agcaatcatt ttcttcaaac actctttctc caattcgtga tcgttattcg
    19201 tactggctaa aagcaatgaa tacccagatt ctcgtaattc attttcgatt cctcggataa
    19261 tcgacggaaa aatataatca gagagatatg tggtgatgac accgatcgtt tttgttctgt
    19321 tcaatggtgt tttttctttc ttatagctgt catctacata tgtgcccgac cctttttctt
    19381 tacgcagata gccctcattt accaacacag cgattgcttg ccgaatcgta tgtctgctga
    19441 cgtcatatat ttcttgcagc ttgaattctg agggaatggt tgtatttact tcgtattcct
    19501 cacgaaggat cttttgtttt atctcttctg caatatcttg atattttgcc atttgccttg
    19561 ccccttttta tttgtacgta catatatcat aacgagtctg caaggtttat tcaagcaaaa
    19621 atcaaggttt cttgtgctca taaggcatac ggaacaggtt caccaaattc tggaaatcct
    19681 tgctcattcc acgtaaagca ttgcgcgttc gcgtgccgat taggattgtc taacggatcc
    19741 cctttttggt tcttctctgg tcgagcatga tagatcaaaa tatcaaagca cccgtcttct
    19801 gaaaccgtaa acgagttgtg accaggacca aattgcttat ttttttcaga agaaacgaat
    19861 actggctccg ataatttatg ccatgattct tttaataaaa gattcttgtg ctcatcagcc
    19921 cacagcaggc ccatggcata gttttcatct gttgcactac cagaatacgt aatgaatatt
    19981 ttcccattgc gagtaaggac tgctggacct tcatttactg aaaaaccccg tttttcccat
    20041 tcatactcag gtatagaaag gaggatctgc ttccccttta acgtccaagg attatccatt
    20101 tcggatagat aaagattaga attcccagta atttcaggat cttgttgggc ccacacataa
    20161 tatagctttc cttgagaaga gaaagttgtt ccgtctaaac tgaaagtttc ccttttcgtt
    20221 tttatttgtc ccttttctat ccaatctgta ttcattggat ctatatgctg attttcaagt
    20281 acgaacatgc ggtgatgatg agattcatca cgtatagttt tatgattgct tgcagcaaag
    20341 taaatatacc attttccatt gatataatgg acttcaggtg cccagatcaa ttcactcatc
    20401 ggtccttctt catgagcctg ccatacaatc aggcttgcag catgttctaa ttcattcagt
    20461 gactttgccc ttcttaattc aatcacttgg tagcctggaa ccgatcctgt aaaataataa
    20521 taaccatctg tatgtttata tacccaagga tctgcccgtt gccgaacgat aggattatta
    20581 taaagtgtct tcatcataac aaatttcctc acattttctt tattctaatt acattccaag
    20641 agtatttttc tatccctaca tttaattcgt catgtttttc tattgaaaaa tagtttattt
    20701 caggttttat tttttctggt tcttcacgtg tatttttgtc caacagatcg ctactggtta
    20761 atgtgtagtg ctctccttga tctagcaaat tgaaatcatg ttcaatcgtt atcttttgtt
    20821 gttcatttgt gcgattgatc aagaaaatag tcagctgatt gccgttatct acgatcacac
    20881 tgtccacata aggaacattt tttatctttt cacaatcata aacagggcaa atgatttgtt
    20941 gtttcaatac ttgaccttta gcatatttcg ataagtgttg catcacataa taagttggat
    21001 tcctccatgc ttgtccttta gcatctgtca gtattaaagg tattgtgttt actaacaatg
    21061 attgacaact aatctttacg cgatctgcgt gtcgtaaaat agccagccct aatgaaccaa
    21121 ataataatgt atcttccata gtaaagtaac accaatcgat gggactaccg acttcaaaat
    21181 ccctatgtct tttttccggt tgggcatgta cattccattc atcaaacgct agatacattg
    21241 ttttctcact tctcaaatat ccttttacat aatcacaagt agcaacgatt tcttcaattt
    21301 gacgatcgaa tcgataagct tttgcaaggt atgtcgctgt atcatcacgc tcatttttag
    21361 gaggtttagt caagtcttct tcatcatact tatcaatata gttatgcaaa gctaaataat
    21421 ctacattctc ataagcttct tctaatgcga tacgatccca ttcagggtac gatggtaaac
    21481 gtggcgtaga gcttccaact aagattgttt caattgaatc atccactaac ttcatgactt
    21541 ttgaggcttc atttgctaac ctaccatatt cataagcact cttagccccc acttgccatg
    21601 gaccgtctag ctcattccct aaacaccata atttaacgcc aaaaggcttt ttttgcccat
    21661 tttttattct taaatcactc cagaaggttc cttttggaaa attacaatat tctactaaat
    21721 tttttgcttc atcaattcct tttgttccta aattcaccgt gaatatcggt ttcgcttgta
    21781 catgactgat ccatttaaaa aattcgtgga gtccaaaaga atttggttct atacctcgcc
    21841 atgctaaatc aatttttttt ggtcgttgtt ctttcctacc tacagtatcc acccaatcat
    21901 aaccagatgt aaagtttcct ccaggatagc gaatgacacc taattctaat tcctttacat
    21961 aagagaggac atcttgtcta aacccgtcac catcagctgt ttcatggccc ggttcataaa
    22021 tccctgtata gacgacactt cccatatgct caacaaatga gccaaacagc cgagaactga
    22081 tctctcctac cgtaaaatcc tctctgattt ttactttcac ttcttcgtcc aacagctttc
    22141 cctacctttc atttcctata gtcaagataa tagatacaag tttccatacg ccaccagcaa
    22201 agataaaaat aaaaggaggg aaaagaaata tgagcagtac aaatcctaat aacaatacgc
    22261 aacaaagaat atttctcaaa aaatcaaaaa atactttcca tgcactttgt tcaaaaattt
    22321 gtaaaaatgt gatatctttg ttttcagaaa tcacatatat ccaggataaa agaaacatgg
    22381 aataaataag taaaactaca tatccactaa ttgaaagtac gattgtctgt tcttctaata
    22441 attgattaac aagaaaataa ataatgatcg ttaacagact aaggaacaag ttcttaccaa
    22501 aatgttgctt tattccttga aagaaaaaat aaatgatatt tcctgatccc tcacctttcc
    22561 attgcgccat tacataaaaa acagcacatg tagcaggcac gatagtaata acaggcaaac
    22621 acacgattac ccagagaata cctaataaga tgtaatcaga aaccacaatc aactgtccat
    22681 aaaatttagt ctcaactatt ttcatcttcc tcactctttc attccagaca tagctgttcc
    22741 ttgaacaaaa tatttctgtg caaaaatata aaccactagt aatggcagca ttgaaataac
    22801 cgttccagcc attacaggtc cataaaaaga aatgttctgc cctgtaaata aagctaaccc
    22861 tgctggtaaa gttctcattt ttgaactact tgtcaatatc aatggataaa gcaagtcatt
    22921 ccaactattc atcagcgtga aaatccctaa tgataaaaga gtaggtttgc tcaatggcaa
    22981 catgattttc aaaaaaatat taaactcact taatccatca atgcgtgctg cctcttccaa
    23041 agaatctggt aaagtaataa aaaaggaacg catcatgaag ataccaaatg cagttgtcat
    23101 acgaggaatg atcaaacctg cataagtatc aagcatgcca agcaaattga cttcaataaa
    23161 tagaggaatc atgaagactt ggaatgggat catcattgtc agcaaaacga aatagaacaa
    23221 aactgatttt cctttgaaac gcatacgagc aaatgcatac cccgcaagag aatcaaagaa
    23281 tacagaagtg atcactgtcc ctccagcaaa aatcactgta tttttgatgt aatctaataa
    23341 agggatcgtt gtccaaacct taatatagtt atctagggta tagctatctg caaatattct
    23401 aggcggaaat gagataatgt ctttttcgtg tttaaaagaa gaaataatca gccacaaaaa
    23461 aggaaacgca ataaccaaca atataactag catactaatc gttcgaatga tttttttacc
    23521 actaactgct tttcgactat tcacagtaac tgtaatcgag ctttctggtg ccatacaggt
    23581 ttccctccta tttcatttgt ttttctttcc gtgacatgta cacattagtg gagatagcta
    23641 tagttgcaac aatgataaac aaaaccgaag acagtgctga tgcatagccc aattcataag
    23701 gtgctgtaaa tcctcgactg taaatatatt gaacagctgt ttctgtctta aattgtgggc
    23761 caccagcggt tgcaacatat acttggtcaa acacttgcat cgaaccaatc agattcgtta
    23821 aaatacaaaa acctaaggag ggaacgatct gaggtagcgt aatgttaaaa aactgctgtc
    23881 gatttgttgc accatccatt tgagcagatt catacaaaga gggagagatt ccctgaatag
    23941 cagtcaagag aatcgtcata gtaacaccaa aattcttcca aacagtcatg atggctaccg
    24001 tcggcatagc aagggttgga tcgcgaaaaa attgagggat cggcaagccg acttgtgaaa
    24061 acaagtaagg aatcgcccct acattcggat caagcaacat agaaaaaata atcgcaattg
    24121 acgtcaatga acaaatacct ggaatgtaaa atgtcgaacg acaaaaacga ttgaatctgg
    24181 tatttttaga taatgctact gctgctaata aaccaacgat aatttgtgtt ggcgtttcga
    24241 gtacggtgaa atataaagaa tgaatcaacg aattaactgc tcgttcatca ctaaatatac
    24301 gtgtgaaatt ttcaaaactt acaaaatttc ttgatgtaaa gaaaatattc aaatctgtaa
    24361 aactaatagc aaatgttcct aaaagcggta tgacagtaaa taataataaa actaaaaaag
    24421 atggagttaa aaacaaatac gcagcacgat caggacgatt ccaatatttg gtgaatccgt
    24481 tcatatagtc cacctcatct ttcattttga taaaatatac aatcttggtt taaacgaatc
    24541 tgaataagat atccgaacaa tcgctgtgat ttttgcaata acacctatct gcaaatacta
    24601 ggtcgatcat tcagatatct gtatcttgcc aacagtctat cctgtataaa gttctgtact
    24661 atttttgcag taattgattg atggcatcat ctgcatcgtt catgagtgtc tcaggatctt
    24721 ctcctgctaa taatttttca atcagtggat tgatgatatc gttattgatc gcagagattt
    24781 gggtaaaacc tggtaaatat ggctctgcat agtccatttg tttcgataat tcagatacaa
    24841 taggatcatt ttttacttct tcatcttttg ctactgaatc caagtaggca ggaaaaccat
    24901 tttcgatact ccatttttta ccaatttcag ttgtattcca atatttcaaa aactcataga
    24961 cagcttcttt tttattatta tctgtacttg ccggaatggc atatcctacg ccatctaaaa
    25021 tagctgtttt tttgtctgaa tcactccctt gtggtaaggt agtcacatta taatcaatct
    25081 cgtttgcttt caatccgtta tttaaccatg gcccattgat ttcaatggct agttgaccag
    25141 cattcattaa attgtccatt tcggcgccag aaatcgattc tggaccagtc ttgtctacat
    25201 ggatcatttt ttggatcgct tttaacgttt taaggttctc aggagacgca aattcagact
    25261 tagtataatc atcgttcact aatttaccgc catttgctaa catccagtta tataacggtg
    25321 cagttccatc cttattgaag acaaaacctg atacattttt actactatca gttaattttg
    25381 gtgccatttc tgtcaattct tcccaagtag taggtggttg ttcagtatct aatccagctt
    25441 gtttaaacaa atcattattc caatacatat acatgccttg tacctgcatg gggacaaaat
    25501 actgtttacc ttctacttca cctaattcaa tagccgtttc tttaaaatca gatttatcta
    25561 ctccatcata ttcccagaaa tcgtctaatg gttgtaccgc tccgttttct acatactgtg
    25621 caaaatctgg ataatttaat gcaataaagt caggtgcttt tttagaagaa atcgctgtag
    25681 gaagtttttc attcaaattg gcccaagtca tgatatccat ttcaattttg atgtttttat
    25741 catttgtttt attaaattca ttaacgattt cttgcaatat ttcaccatca gatgcagtaa
    25801 acccattcca aaaagtcaaa gtcacttctt tatcagaatc agattctgct gaacttccgc
    25861 caccgcaagc tcctaaaata cctgttgcag ctaccaacaa agagccaatt aaccattttt
    25921 tacctttcat tttttgttcc tccttcatct gataaacgac atctgaccac attccaagat
    25981 agcggtttca ataaacaatt gattttccca tcttcagctt ttacatttgt tgaatgactt
    26041 ggttttactt gttcactttt aggaagatta gttgcttttg tatcaaatcc actcatttca
    26101 gaaaaatcaa tcactccctc taatgataat tcagacaagt gaacagttag attcatttct
    26161 tcttctccac ggttaactgc aaatatgaca agttctttgt catttattac ggctatactt
    26221 tctaaatacg gcacttcttc aaaatcttcc gtcgcatagt ttgctgaacg aatttgtggg
    26281 cttaacacct ctccttgtcc atacacagca gcttgcataa atggataata gatcgtctgt
    26341 ctccaagctt ctccgtttgt ttc
//
LOCUS       DACEPW010000018        26166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEPW010000018 DACEPW010000000
VERSION     DACEPW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.018"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(130..825)
                     /locus_tag="IW819_000579"
     CDS             complement(130..825)
                     /locus_tag="IW819_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1243708.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IW819_000580"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IW819_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1243709.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="IW819_000581"
     CDS             complement(2024..2215)
                     /locus_tag="IW819_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1243710.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="IW819_000582"
     CDS             complement(2236..3126)
                     /locus_tag="IW819_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1243711.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="IW819_000583"
     CDS             complement(3113..3880)
                     /locus_tag="IW819_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1243712.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IW819_000584"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IW819_000584"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1243713.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IW819_000585"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IW819_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1243714.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IW819_000586"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IW819_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1243715.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="IW819_000587"
     CDS             8496..8834
                     /locus_tag="IW819_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243716.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="IW819_000588"
     CDS             complement(9002..9760)
                     /locus_tag="IW819_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1243717.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="IW819_000589"
     CDS             complement(9778..10596)
                     /locus_tag="IW819_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1243718.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IW819_000590"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IW819_000590"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1243719.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IW819_000591"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IW819_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1243720.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="IW819_000592"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="IW819_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1243721.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13256..14386
                     /gene="dnaN"
                     /locus_tag="IW819_000593"
     CDS             13256..14386
                     /gene="dnaN"
                     /locus_tag="IW819_000593"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1243722.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14615..14857
                     /gene="yaaA"
                     /locus_tag="IW819_000594"
     CDS             14615..14857
                     /gene="yaaA"
                     /locus_tag="IW819_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1243723.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14844..15968
                     /gene="recF"
                     /locus_tag="IW819_000595"
     CDS             14844..15968
                     /gene="recF"
                     /locus_tag="IW819_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1243724.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15965..17911
                     /gene="gyrB"
                     /locus_tag="IW819_000596"
     CDS             15965..17911
                     /gene="gyrB"
                     /locus_tag="IW819_000596"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1243725.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17931..20402
                     /gene="gyrA"
                     /locus_tag="IW819_000597"
     CDS             17931..20402
                     /gene="gyrA"
                     /locus_tag="IW819_000597"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1243726.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20638..20937
                     /locus_tag="IW819_000598"
     CDS             20638..20937
                     /locus_tag="IW819_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1243727.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20987..21499
                     /gene="ssb"
                     /locus_tag="IW819_000599"
     CDS             20987..21499
                     /gene="ssb"
                     /locus_tag="IW819_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1243728.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21525..21761
                     /gene="rpsR"
                     /locus_tag="IW819_000600"
     CDS             21525..21761
                     /gene="rpsR"
                     /locus_tag="IW819_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1243729.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21904..23877
                     /locus_tag="IW819_000601"
     CDS             21904..23877
                     /locus_tag="IW819_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1243730.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23883..24335
                     /gene="rplI"
                     /locus_tag="IW819_000602"
     CDS             23883..24335
                     /gene="rplI"
                     /locus_tag="IW819_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1243731.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24609..25976
                     /gene="dnaB"
                     /locus_tag="IW819_000603"
     CDS             24609..25976
                     /gene="dnaB"
                     /locus_tag="IW819_000603"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1243732.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         7943 a         5153 c         5188 g         7882 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cagcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattca atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact
    13201 tttattattc tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa
    13261 agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc
    13321 aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt
    13381 gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga
    13441 aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag
    13501 cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca
    13561 agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta
    13621 tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac
    13681 taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac
    13741 aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg attctcaccg
    13801 tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc
    13861 tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat
    13921 cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt
    13981 gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt
    14041 tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga
    14101 aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa
    14161 ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc
    14221 attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat
    14281 gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga
    14341 tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat
    14401 gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta
    14461 aaatctcttt taaggctttc tgtttatttt tgtttaaatc acctttttta gattaaatcg
    14521 tcttatatgg aaaaatatat ggataaattt gttttattgc gaaaaaaaag gtataataga
    14581 gtatactttt aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac
    14641 ggaattcatg acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca
    14701 agcaaaatgg tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg
    14761 tggacgcaaa ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat
    14821 ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa
    14881 attacgaaga gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg
    14941 cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc
    15001 ggacaagtag tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag
    15061 agatcacaaa aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa
    15121 aaaccaagat caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg
    15181 tcattttatt tgcaccagaa gatctttctt tagtaaaagg cagcccgcag atcaggcgga
    15241 agtttttaga tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat
    15301 accaagcaat tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa
    15361 ctgatttgct ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat
    15421 tgaaagcaag agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc
    15481 aaatcaccca tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag
    15541 aaacacatac gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa
    15601 agaaagacct ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt
    15661 ttattaatca gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac
    15721 taagtgtcaa attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc
    15781 ttttgttaga tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa
    15841 caatcgaagg aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata
    15901 aaatgactgt tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa
    15961 ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat
    16021 tcaagtactt gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac
    16081 tagttctgag ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt
    16141 attagcaggt tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt
    16201 tattgacgac ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt
    16261 tgaaacggtc tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt
    16321 ttctggtgga cttcacggag taggatcttc tgttgtaaac gcgctttcta cacagcttga
    16381 tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt
    16441 ggacgactta aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc
    16501 agaccctgaa atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat
    16561 ccgtgaactt gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga
    16621 agaaccagtc atcaatgaat accattatga cggcgggatc aaaagttatg tcgaatactt
    16681 gaatgcaaac aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga
    16741 tatcgcagtt gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt
    16801 tgccaacaat atccatacgt atgaaggtgg aacacatgaa tcaggattca agactgcttt
    16861 aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa
    16921 cttgaccggt gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga
    16981 ccctcaattc gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac
    17041 tgatcggtta tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg
    17101 gcaaattgtt gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg
    17161 tgaagtaacg agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga
    17221 ctgctctagc aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg
    17281 cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa
    17341 aatcttgaat gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc
    17401 tttatttact gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta
    17461 ccacaaatta gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct
    17521 gctgactctt ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc
    17581 tcagccacct ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa
    17641 aaatgcagaa gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag
    17701 cgttcagcgt tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat
    17761 ggacccaagc aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca
    17821 gatatttgaa atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa
    17881 tgcacactat gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag
    17941 aaatcagaga aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg
    18001 attatgcgat gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac
    18061 ccgttcatcg ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc
    18121 ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagaca
    18181 ttgcgatcta tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag
    18241 tggatggtca tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata
    18301 ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg
    18361 ttgatttcca aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct
    18421 tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc
    18481 cacctcataa cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg
    18541 tgacgaccaa tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg
    18601 ttatggggaa atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac
    18661 gtgcaaaagt tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat
    18721 tgccttatat ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca
    18781 agcgtatcga agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg
    18841 tcattgacat ccgtcgtgac gccagcgcat ccgttatctt gaataactta tataaattga
    18901 catccttgca aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag
    18961 ttttgagctt gaaacaaatc cttgaaaact atatcgaaca tcaacgcgaa gtcatcacac
    19021 gacggacggt ctttgaaaaa gaaaaagcag aagcaagagc acacatctta gaaggtttgc
    19081 gtattgcttt ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg
    19141 acgaggcaaa agcaacaatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta
    19201 ttttagatat gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat
    19261 accaagaact cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg
    19321 ttacagaaat catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac
    19381 gcacagaatt actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag
    19441 aagaaatcgt tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat
    19501 tcagagctca acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact
    19561 tcgtcaaaaa tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg
    19621 gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata cgggcgtaca gccaaaggaa
    19681 tcccaatcat caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg
    19741 tgacgggaga agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa
    19801 aacgtacttc tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt
    19861 tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta
    19921 ttggaacaca taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac
    19981 gtacagcttc cggggtcaga gggatacgcc tgcgtgagga agattatgtc gttggggcag
    20041 ccttaatgaa ggaaaatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga
    20101 caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca
    20161 tcactgaaaa aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca
    20221 tgctgattac agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg
    20281 gacgttcaac attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga
    20341 tggcagcagt cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat
    20401 aaccatcaag actaagtgtg taagctagca agaataaccg attaaaaaat ctggttattc
    20461 ctgctggctt ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta
    20521 ttgcttttga ccaaaataga gtgtataatt agagcttgtg agtagtctaa ttaattagat
    20581 aactctcctt gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg
    20641 gaaaacacga aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa
    20701 actgctttaa tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa
    20761 tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc
    20821 tatcacatcg ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt
    20881 cttgctaaaa tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg
    20941 ttgtttcatg tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt
    21001 tgtattagtt ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc
    21061 agtcgcaaca tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga
    21121 ggctgatttt atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc
    21181 tcgtaaagga acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca
    21241 gcaaggacaa cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag
    21301 tcgttctgca tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc
    21361 tggcggattt ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc
    21421 tgatttcgac cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga
    21481 cgatgattta ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa
    21541 gaggcggacg taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg
    21601 attataaaga tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac
    21661 gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg
    21721 ctcgtatcat gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat
    21781 gactgatcct tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg
    21841 tttagtcgct cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa
    21901 attatggaaa ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata
    21961 ttaatcgaag tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt
    22021 ctattgatga atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta
    22081 aataaacgtg aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc
    22141 tcagaagtca tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg
    22201 ctcaatccat tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta
    22261 gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca
    22321 tttcagtttg atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac
    22381 cagttgttaa aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat
    22441 aactatgcag atgccattga taaattagat gacaaagaga tttcttattt gaactctttc
    22501 gtcactacac tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac
    22561 gcagaacggt ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa
    22621 tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt
    22681 agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat
    22741 aatttagatg ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa
    22801 ggagcaaaac ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt
    22861 cgttctcgag cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt
    22921 atcatggggc acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt
    22981 ctggcacgct tcaaccagaa aaaagcttac atcgttatca atgagaacga gatcattcca
    23041 gatgtagaac ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc
    23101 tctccagaac gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac
    23161 cataaacctt ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc
    23221 attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatattgaa
    23281 tcttcagctt cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa
    23341 acaaatcgct tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca
    23401 aatagtttca atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct
    23461 tgtggagcag acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg
    23521 gagatcagca atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca
    23581 gggacagacg aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt
    23641 tcgatgatca atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg
    23701 atcagtgcca gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc
    23761 ggaggacact tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa
    23821 cagaagctat ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc
    23881 aaatgaaagt aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag
    23941 aagttccagc aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa
    24001 cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg
    24061 aaatcttgga agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg
    24121 aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa
    24181 ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag
    24241 aaaatccgat ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga
    24301 cggctacgat caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct
    24361 ggtaagtata accagaagga aaaaagagaa accgaaattt atctgagaaa acagctgcag
    24421 caatgaaagc aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca
    24481 gcaaaatagc ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc
    24541 ggtacgaatg atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag
    24601 cgatacgtat gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac
    24661 aagcggtttt aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca
    24721 tagagccaaa agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt
    24781 taaatgatcg taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca
    24841 atttgttaga agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga
    24901 cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat
    24961 tgatccagac agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa
    25021 caattttaga tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg
    25081 gctttttatc tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc
    25141 aaaacaatga agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag
    25201 caggtttcca gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag
    25261 cgtttgcatt gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat
    25321 ttagtttgga aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga
    25381 ttgaggccag ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg
    25441 ttgcgatggg gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa
    25501 tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga
    25561 tattgatcga ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag
    25621 tatcagatat ctctcgtcaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg
    25681 ctctttcaca gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg
    25741 atattcgtga atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag
    25801 atgattatta tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca
    25861 acgtcatcga agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac
    25921 tgtttatcaa agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa
    25981 gaggttgtga gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc
    26041 aggaacgact agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg
    26101 tcacagtccc tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag
    26161 caaacg
//
LOCUS       DACEPW010000019        25558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEPW010000019 DACEPW010000000
VERSION     DACEPW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.019"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            420..662
                     /locus_tag="IW819_000604"
     CDS             420..662
                     /locus_tag="IW819_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ1243733.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            659..1129
                     /locus_tag="IW819_000605"
     CDS             659..1129
                     /locus_tag="IW819_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1243734.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1257..2471
                     /locus_tag="IW819_000606"
     CDS             1257..2471
                     /locus_tag="IW819_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1243735.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(3181..3369)
                     /locus_tag="IW819_000607"
     CDS             complement(3181..3369)
                     /locus_tag="IW819_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243736.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            3460..3669
                     /locus_tag="IW819_000608"
     CDS             3460..3669
                     /locus_tag="IW819_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1243737.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3781..5370
                     /locus_tag="IW819_000609"
     CDS             3781..5370
                     /locus_tag="IW819_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1243738.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIY"
     gene            5429..6640
                     /locus_tag="IW819_000610"
                     /pseudo
     CDS             5429..6640
                     /locus_tag="IW819_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6663..7589
                     /gene="glsA"
                     /locus_tag="IW819_000611"
     CDS             6663..7589
                     /gene="glsA"
                     /locus_tag="IW819_000611"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1243739.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7797..9257
                     /locus_tag="IW819_000612"
     CDS             7797..9257
                     /locus_tag="IW819_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243740.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9478..11241
                     /locus_tag="IW819_000613"
     CDS             9478..11241
                     /locus_tag="IW819_000613"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1243741.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11325..11783
                     /locus_tag="IW819_000614"
     CDS             11325..11783
                     /locus_tag="IW819_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1243742.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11892..12575
                     /locus_tag="IW819_000615"
     CDS             11892..12575
                     /locus_tag="IW819_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1243743.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12722..13336
                     /locus_tag="IW819_000616"
     CDS             12722..13336
                     /locus_tag="IW819_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1243744.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVLALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13352..13558
                     /locus_tag="IW819_000617"
     CDS             13352..13558
                     /locus_tag="IW819_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243745.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            14099..15229
                     /locus_tag="IW819_000618"
     CDS             14099..15229
                     /locus_tag="IW819_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1243746.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15321..16076
                     /locus_tag="IW819_000619"
     CDS             15321..16076
                     /locus_tag="IW819_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1243747.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            16134..18173
                     /locus_tag="IW819_000620"
     CDS             16134..18173
                     /locus_tag="IW819_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1243748.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18282..19433)
                     /locus_tag="IW819_000621"
     CDS             complement(18282..19433)
                     /locus_tag="IW819_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1243749.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19587..20117)
                     /locus_tag="IW819_000622"
     CDS             complement(19587..20117)
                     /locus_tag="IW819_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243750.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20216..20566)
                     /locus_tag="IW819_000623"
     CDS             complement(20216..20566)
                     /locus_tag="IW819_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1243751.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20723..22252)
                     /gene="ahpF"
                     /locus_tag="IW819_000624"
     CDS             complement(20723..22252)
                     /gene="ahpF"
                     /locus_tag="IW819_000624"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1243752.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22262..22825)
                     /gene="ahpC"
                     /locus_tag="IW819_000625"
     CDS             complement(22262..22825)
                     /gene="ahpC"
                     /locus_tag="IW819_000625"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1243753.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(22969..23631)
                     /locus_tag="IW819_000626"
     CDS             complement(22969..23631)
                     /locus_tag="IW819_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1243754.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23815..25461
                     /locus_tag="IW819_000627"
     CDS             23815..25461
                     /locus_tag="IW819_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1243755.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25452..>25558
                     /locus_tag="IW819_000628"
     CDS             25452..>25558
                     /locus_tag="IW819_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243756.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMD"
BASE COUNT         8689 a         4366 c         5090 g         7413 t
ORIGIN      
        1 agccgttcaa agcccccgtt atctaacaag gggggcacaa aaaacaagaa acactataca
       61 ctaacaagcg gaaaacgctg tatttataag gcaaaacccc atttttacag tagtgatata
      121 aattgtacct atatcactat gaaaattgaa tagtaaatat gagaaagaca gttgatttca
      181 aaaaataaga aattgattgt cgttttcttt tgcagaaatt taagtgcagg caattatggt
      241 agttgtcgta attgctgtca gctcagtgtt tatcactgat ggtgtgaaag atttacggtt
      301 ggagatgttc aataagatta ttggtgctta attattttta ttgtagtgct gatatgagaa
      361 tgttataatt ggggtataaa acatacaaat aaatttgctg aaaaatgagg tatcaaaata
      421 tggaaaatga attactggaa aacttaggaa gacttcacac aactgaactt ggcgttatta
      481 gaattaaaaa gaacttatct ttgaatgtag aaaatgtaat tgaatggtgc aaagaaaaaa
      541 ttagcctttc taatgcggaa atcacaagaa aaggaaaaaa ctggtatata acgattgata
      601 attgtattat aacggtaaat gcgtacagtt ataccgttat cactgctcac aaggtaaaat
      661 gataaatttt tctataaagg aattaaacgc atttgcagtt attgggcagg aagtagaact
      721 gactaattat caaaaaaaga atattcagat tagtacacag ttttggagaa agtttaacag
      781 tagtttgaaa aaagcgtatc tttcacaatc aggaaattgg gtaaaatatg cttttatgga
      841 aagaagaaat ggaaaacttt attatttctg ttctattccc caaagaacca ttaccccaga
      901 gggctttctg tataaagaaa taccgtctta taaatatttg gttgtggagc atattggtgc
      961 tatggataaa atatatgaaa cttatggcaa gatttatgaa gaaattattc ctagtacatc
     1021 gtatattcct ataaaagata ttatcctaca ctttgaaaaa tacgattatc gttttcattg
     1081 gaatagtgat aattcagtta ttgaaatttg gatacctatt caagattaga agcaatttta
     1141 tatccataca cacaaagcag ttagtcttag gattgactgc tttttctata cagattttta
     1201 atgacaggca attatcaatt atggtagttg ccttttttga ttggaggaat tgaagaatga
     1261 atgataaaaa ctttattgaa gaactaagac aaaagcgtga ggaatacgga gtaacacaaa
     1321 caaggcttgc tgttgcctgt ggtatcagcc gtgaatatta caaccgcatt gaaaaaggaa
     1381 aacagccatt gaatgacgaa ttaagagaaa tcatagaaaa acagattgag cgtttcaatc
     1441 cgcaagaacc actattctta ttgattgatt actttcgtgt ccgctttcct actacggacg
     1501 cattggcgat tatccgtgat gtattacaac tgaaatctaa ttacatgctt tatgaagatt
     1561 atggaaaata cggatatgaa agcaaatatg tacttggcga catcaatatc atgtgttcca
     1621 tgcaggagca tttaggtgtt ttattggaac tgaaaggcaa aggctgtcgg caaatggaat
     1681 gttatctgct ggcacaggaa cgctcatggt atgacttcat gctggattgt atgacggctg
     1741 gtggtgtgat gaaacggctt gatttggcta tcaatgataa agcaggaata ttggatattc
     1801 caaagttaaa ggaaaaatac aaggctggcg aatgtatgtc ctacttccgt atgcagaaag
     1861 attacagcgg tacagaaaaa tgcggtagcg atttaccaaa gaatacagga gaaaccttat
     1921 atctcggttc aacaagtagc gaattatata tgtgtgctta tcagaaaaat tatgagcagt
     1981 atgtcaagaa tggcacagaa attgaagata cggagattaa gaaccgtttt gaaatacgca
     2041 tgaagaacga acgagcctat tatgcggttg tagatttact gacctatcgg gacgctgaac
     2101 gcaccgcttt ttctatcatc aatcattatg tccgctttgt tgacagagag gacgacaagc
     2161 caaaaagtca atggataaca aatgatgatt gggcatggtt tgtaggggaa aacagagagc
     2221 cgatacgctt aaccacaaag ccagagcctt acactttaca aaaagcgttg cattggctac
     2281 aaagacaagt cgcaccaacc ataaaaatgg tacaggcatt agacagagaa aatcgtacaa
     2341 cgatactgaa agatatgatt gagcaggcag aactaaaaga caagcataaa catttattac
     2401 aattagagaa atcaaccata gaagaacgta tagataccgc tgttccacaa gagaatgacg
     2461 gtatttttta atgtaattga aaaatttcag atttagtcag caaagagctg ttttcattcc
     2521 ctatgtgaag tgaaagaaga aaaataaatt ttttattaaa tctgcaacaa aatggctact
     2581 tgcgtacaga tatggtgcga gaaaaggaaa tctaaacttt ttttgaaaat aggtcattaa
     2641 atcggggctt gctgtcccta tatgaagtga aagaagaaaa aagttttgaa aaaatgacgg
     2701 aaattcgtga ttagtggaca gtcttctgtg aaaagaggaa aaacatcatc attttgtatt
     2761 caaacaggca ttagcagttg agtgttgatg tgaaaagatg aaaatacttt gttctcaact
     2821 taacaattta aggtttccgt tccctatatg gtgcaaagaa aatatttcat tctattgttg
     2881 gcagtaacgg tatggtttgg gtagttaggg tgtgaaaagg aaaatattct tttttcatca
     2941 tcaacttagc aaaatgacat tttctatccc tatatggtgc atgaaaagaa gttaaaattt
     3001 ttccatttaa acttagcaaa tggacgtttg ctgtacagat agtagtgaaa aaggagaaaa
     3061 tcaaccgttg attgccaaaa tgaaccgttc catttgtact aataatggaa aaagttttgg
     3121 attttggtta cgtttctcct cttttccaac gcgatatata ggaaagacga tttttttctt
     3181 tcaatcgttc tttgacaact gaataaagct attcaatacg tttgacagac agctagccgt
     3241 tgaaacaaat acgccacgac ctttcccctg caagagcgag cgaaaaccta tttgtgttct
     3301 tgtaaaagat ttgtcctctt ttatcgccat gacctgtact gtcagataat gatactcccg
     3361 tacagccaca gcttgagcat taagagcgtc gcacgcaatg ggtacggcta cataagaact
     3421 atgcagaggt ggaactcctg tgggctatga caaaagccgt tgaattgctt agaaagattt
     3481 tacagaaagg gggtatgtgt tattgataat gctgtaaaaa caattcaaaa gaaaaatggc
     3541 aagtgtggca ttggcaacag aggaaagaca aagattgtag taaaagagca tttttccgaa
     3601 aagggcaaaa caatggaaga acttctaact gatgtcatgc tggaaaaagc aaagcagaca
     3661 atcgcataaa atcggtgcag ttgtaagaaa tagattgaat gacaaaaagt acccgtgtta
     3721 tactttactt gcaagcaggt attgtaaaca cgggttagtt ataaaggagg ataactgaca
     3781 atgaaacaac agatttacaa tactgcactt tatcttaggt taagccgaga tgatgaatta
     3841 cagggcgaaa gttccagcat taccacacag agaagtatgt tgcgtttata tgcaaaagaa
     3901 catcatttga atgtcattga tgaatatatt gatgacggct ggtcgggaac aaattttgac
     3961 agaccgagtt ttcaaagaat gattgaggat atagaggcag gaaaaatcaa ctgtgttgta
     4021 acgaaagacc tttcccgtct tggcagaaat tatattatga caggacaata tacagaattg
     4081 tatttcccta gtcataatgt ccgctacata gcgattgatg acggtgtaga cagcgaaaaa
     4141 ggcgaaagtg agattgcacc atttaagaac atcatcaatg aatgggtggc aagagataca
     4201 agccgtaaag tcaaatcagc atttaagaca aagtttgcgg agggggctca ttacggtgct
     4261 tatgctccgt taggatataa gaaacaccct gacatcaaag gaaaactgtt ggttgatgat
     4321 gaaacaaaat ggattattga aaaaatcttt tccctagcct atcaaggtta cggaagtgca
     4381 aaaatcacaa agcagttaag agcagaaaaa gttccgacag cgtcatggct aaattttaca
     4441 agatacggta cttttgcaca tatctttgag ggaaaacccg aaagtaagcg ttatgagtgg
     4501 acgattgctc atgtcaaggc gatattgaaa agtgaggttt atatcggaaa cagtgttcac
     4561 aatatgcagt ctacggtatc gttcaagagc aaaaagaaag tgcgtaagcc cgaaagtgaa
     4621 tggtttcgag tagaaaacac tcacgagccg attattgaca aggaagtgtt ctatcgtgtg
     4681 caggagcaga taaaatcaag acgcagacag acaaaggaaa aggcaacgcc gatttttgca
     4741 gggcttgtca agtgtgcgga ttgtggttgg tctatgagat ttggaacgaa taagacaaat
     4801 aaaacgcctt acagttatta tgcttgcagt tactacgggc agtttggcaa aggtaattgt
     4861 tctatgcact acattcgtta tgatgtgctg tatcaagccg tattggaacg cttgcagtat
     4921 tgggctaagg cagtacagca ggacgaagaa aaggtattga acaagataca gaaagctggc
     4981 aatgcagaga gaatacggga aaagaagaaa aaggcaagta cattgaagaa agccgagaac
     5041 agacaaaatg agattgaccg tttatttgcg aaaatgtatg aagatagagc ctgtgagaag
     5101 ataacagagc gaaattttgt gatgttgtcc agcaagtacc aaaaagaaca gatagaactg
     5161 gaacagcaga taacaagcct aagagaagaa ctaagtaaaa tggaacagga tatgataggt
     5221 gctgaaaagt ggattgagtt aatcaaggaa tattccgtac caaaggaact gacagcacca
     5281 ttattaaatg ccatgataga aaaaatcctc atacatgaag caacaacgaa tgaggataac
     5341 gaaagaatac aggaaattga gatatattag cgatttattg gaagagttga gtaatcaaca
     5401 agagtaatat ttttaactaa gggaaaccgg cggtgtctat acttgggtca gtcgcacatt
     5461 gggaaaacga ttcggttttg ccgcaatctt tttccaatgg tttgagataa cagttggcta
     5521 cttgaccatg ctgtatttct taactggtgc attatcctat gcaacgggta ttggtgctat
     5581 acaaaacaat aaatatctga aattggcaat cctattgatt attttctggg ctattttact
     5641 ctcacagtta aaaggaacca aatacacttc attgattgct cggatcggtt ttgtagctgg
     5701 tatcttgttg ccagcattag tcttgtttgg cttagggatc cattatgtcg cttcaggcgc
     5761 tccactacaa acgatactgt ctatgaaaac attgattcct gatttttcga aattgcctac
     5821 gctggttgtt tttgtttcct ttattttagc atatatgggc gtagaaacat ctgccagtca
     5881 tgcaaatgaa atggaaaacc ctaaaaagga ttatccatta gcaatgtttg tattggttat
     5941 tctggcaatc attttagata cttttggcgg attgaccgtc gctactacag tacctcaaca
     6001 tggtttgtct cttaatacag gggtgattca atcgttagaa ttcctattaa gacacattga
     6061 tccaaatctt gcatggattg cgaaaattct tgcgattctt gtttgtttag gtgtcatcgg
     6121 agaaattgct tcttgggtaa cctctccttc aaaagcactt cacgtagcag cagaagaagg
     6181 attacttcct gaatattttg caaaagaaaa cacacacggt gttcctgctc atttgatgat
     6241 cgcaaatggg atcgttgcaa caatctgggc agccgtcttt acattaagcg gcggggggaa
     6301 taatctttca tttcttgcag cgatgtcatt gactgttgta atctacctga tgttgtactt
     6361 tatgtttttc gcgggctatt tgaagctggt ttttaaagat gacagtcttg aaagagccta
     6421 tcaggttcca ggcggaaaag ttgtaaaagt aatcgttgct tgtttaggtc ttttgacttc
     6481 actttttgct ttttgtatct catttgttcc acctgctagt atcgcagctg gacagcatgg
     6541 gatttatgaa acgattctga ttgtttctta tcttatcgtc ttagttattc catttacgat
     6601 ctacgcatgc cgtcataaat tccagcatgc agaaaaataa aaaatgaaag ctggttattc
     6661 aaatggaagt aaaagaatta gaacaattga ttggaaaaaa tttccactat tatcaaacag
     6721 gacaagtggc ggattacatt ccagcattag ctgaagtgga tcccaaacaa ttaggtatgg
     6781 cgatttatga tttggataca aaacaactga ttgagggagg agacgccgat gtgcgctttg
     6841 ccatcgaaag catgtcaaaa gttcccgtat tgttattagc aatcaaagac aacggaatag
     6901 aaaaagtctt tcaaacaatc aacacggaac caaccggttt tgccttcaat tctatcatga
     6961 atatggaaat caatcatcgc aaacatccaa tgaatccttt tgttaatgca ggagcgattg
     7021 caacaacctc gttgattgcc ggtaaagatg cagatgaaaa atttgagcga atcttgtcat
     7081 ttatgaaaga gatctgtgat gatcctaata tcacattgaa tgaagaaatc tatcattctg
     7141 aatcaagaac aggtgatatc aatcgctctc ttgcttacta tatgaaaggt aatcaaatga
     7201 tcgaaggaga cgtaccggag atactcgatg cttatttcag acaatgctcc ctcaatgtca
     7261 ccgctacagg catagcaaaa ttagctgctg tgctggcaaa taaaggaatt gctccttgga
     7321 atggcaaaca aatcattact gaagaaagtg ctacaatcgt caaatcaatt atgacaactg
     7381 ctggtttgta tgatgaatca ggagagtttt ccgtccatgt tggtgtacct gcaaaaagcg
     7441 gtgtaggcgg cgggttgatg gcagctgtgc caaatcgtta cggcataggt gtcttcagcc
     7501 cggcactaga tccatttggt aacagcgcag ctggtatcca attgttgaaa gatgtagtca
     7561 aagaactaga cgctgacatc tttgaataac cattcttgaa agaggctggg gtaaaagtcc
     7621 aaacggctca aagctaccta caatgaggct gaaattgtga gcttgtttga cttttgtccc
     7681 agtttctttt tttattggtt tttgaaaggg cttttctaaa taaaaaggaa acaaaaatac
     7741 gatttacaga tatgattaaa taaaatcata agaaaaactg gaggcgtgta tcaggaatgc
     7801 tggaaaatta catcgagcga aatatattta gaaaagtgta tctgtgtgaa caattatttg
     7861 aattccaaga aatagacatc gaacaaacag ctatttctct aagggtgact acaccaacga
     7921 tccttcacga tctagaaagt ctagccgagt gtctagaata ttgtataaaa gaacaagtaa
     7981 gagagaagca taagtataaa ctcgtattca aacatggtat tgctctttct gagctcacac
     8041 agtttctgta cggtcaatct tattttttaa agtttttaag ttattatttt aatggtatat
     8101 tcacgagtac agaactggct gatcttgaat ttatttcttt gagtaaagta tatacaatca
     8161 aaaaaatcgt attggatttt tttaaagaaa atgattattt gaaaaataaa gaaatcatta
     8221 tcccagaatt tgactcgcgg aacatcttgc tagctttagt acgttatgtc aattgggaag
     8281 gctatgaaaa taaaaatccg tatgtagaag aagcttgtaa cgagttgatt gattatgtag
     8341 agactcattt ttttaaaaga agatattcgg aagatgaaaa gtcctttatc atacgagaaa
     8401 tagagatcgc aatcggaaga aaggcatttc cccttggact gaaagaagaa gataagaaaa
     8461 gcgcagaaac gaagccgtta tttcaattag tctgtcaagg attggaagag caaaaaggaa
     8521 ttctagactt acaagaagaa gatgtctatt atattttttc tttgttcaac agtagaaatt
     8581 ataccaatga gaacatggag ctgttaagaa aagatttcga agttgtttat aaaaattttg
     8641 ttctcaatga cctatcactg aatgaattga ttttacagat tattagtatc gctgaaatcg
     8701 acgatgcaga aaattttatt ttcaagcaat cctttttaca atttgtccgt acgttgtggg
     8761 cagatggaca agtattttta ccggaaagga tctatcttct atcagaaaaa gaacaacttc
     8821 tgtacggaaa aattgtagaa atattggaag agtggaaaaa gaaaaatcaa atccaactaa
     8881 gatggaatga aaatttcatt cgaaaattcg tcaaaagtct ttctttgatc aacagtgaac
     8941 aagcggggag acaggaaata gaaatttttg ttgtttcaga aagttcagtc aaacaatttt
     9001 tttatcgaac ccaatttcat ttgtatgcgg aagaaggcat cgctgaaata aatcctattt
     9061 tatttcgttc attagaagaa ttgcctgatg aatgtctatg tgcgaaaaaa agagtggttt
     9121 tgtgcgatgc tgctagctat caaaatggat tagaaactga aaagacaaag atctatccaa
     9181 tctcattaaa aggtgcaaac atccatttgt atcatattgc tcaggaactt cacctattaa
     9241 aaaaacatat ttcttgatga gaccttaatt aggagacctt ctacaaagct ttttaattgt
     9301 ctgtagaagg tttttatttg agtcctgatt cgtgcgaaga tctacttata taacaagctt
     9361 tctctgaaat ttttttaaaa tgctattgac ttagagtcaa ctctaagtag tagaatagat
     9421 aaagtgtaac aaatctattt tcattaaaag aagatagtac ggaggagaga atgtattatg
     9481 agtacagtat tacatgtatt ggatgcaatc aatagccccg cagatgtcaa aaaattatct
     9541 gttgaggaaa tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca aaaggacagc
     9601 gatgtaggtg ggcacgttgg tccgaatcta ggtgtagtag aattaacgat tgcgtttcat
     9661 tatgtatttg attcaccaaa ggataaaatc gtttgggatg tttcccatca gtcttatggt
     9721 cataagcttt taactggtcg aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc
     9781 ggatattcca accaacatga aagtaaacat gactatttta cagtggggca cacatctaca
     9841 tctatcagct tagcaacagg tttagctaaa gcgagagatc tgaagaaaga atcaggaaat
     9901 gtggtagcat ttatcggtga cggctcccta agtggtggct tagcttttga aggattgaac
     9961 aatggtggtg ttttacaatc caatctaatc gtattagtaa acgataatga gatgtctatt
    10021 gatgagaatc atggcagttt atataaaaac ttagctgagc ttcgtcaaac caacggtaaa
    10081 agcagcaata acctatttaa agcaatggga tttgattatc gttacgtgga taatggaaac
    10141 gatctggaaa cggtgatccg tttatttgaa gaagtaaaag atattgatca tccgattgta
    10201 cttcacgtcc atacggaaaa aggtcgcggt tatcagccgg caatcgataa taaaatgaaa
    10261 tatcattggc atgtaccatt tgatctgtcc accggggagg caaagcaagt aacttctgga
    10321 gaaacattta atatgatcat catggattat ttggataaga aaatgcaaac aagtacagct
    10381 cctattgccg cagtcaatgc agcgatacca ggcgcctttg gactgaaaac attccaagaa
    10441 aaacatccag atcattattt tgacgtgggg atcgctgaac agcactctat cactttcgct
    10501 tctgctttgg cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa
    10561 cgggcatatg atcaattatc acatgattta gcgatcaacg agaatccagt gattatttta
    10621 gtcggtggcg gaacgatctc agctagtgac cgaacacacc aaggaacatt tgacattcct
    10681 tacttatcaa gtatcccgaa catcaaatat cttgctccag caacaaaaga ggatatgatc
    10741 tctatgctag attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg aattcctaca
    10801 catggtgtag aacatggtac agctatcgca aatgattata gtattccagc ctatcaaaca
    10861 gtaaataatg gaggaaaagt agcaatttta gctttaggcg gttttcttga attaggaaaa
    10921 gaaacagtaa aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg
    10981 atcacagaaa atgacgtaga aacattagat aaattaaaag aaacacacga attagtcatc
    11041 acattagaag atggtagttt gtcaggagga tttggcgaaa atatcagtcg ttattatggt
    11101 gattcttcaa tgaacgtatt aaattttggt gcagagaaag aattcacgga tagcgttcct
    11161 gtagggcagc tttatgaacg ttatcattta acaccaaaac aaatcgttga agatagtctg
    11221 cgtgtcttga atattcaata attcattatg ctcaccatcc agataattgt ttatactagt
    11281 atctggatgg ttttttcatt ataaaaagca aaggggtgga tgttatgtat cagcaagtag
    11341 aagaatatgt caaaaagttc caagaaaaat atcctagtta tcaatcagaa aaattaggtt
    11401 tcttttcatt tggggataca gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa
    11461 agataaagac tggtacatca agtggttacg atctgtataa acaagagaat gagcctttac
    11521 caaaagttgg tcaattagac gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca
    11581 aaaatataaa tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat gcttggaaag
    11641 aaggagaagg ggatcggaca ttagcttatt ggcggaaagt ccatgctgag ttttttaaac
    11701 atgcttataa agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag
    11761 aatttgaagt aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt
    11821 ctcctgaata gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg
    11881 gaggagatat tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag
    11941 ctttggaaaa cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa
    12001 aagaaggcgg agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc
    12061 gaatcgttga tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt
    12121 ttattactgc gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg
    12181 cactggatgc aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa
    12241 tgattcacga acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc
    12301 aagcgcaaga agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt
    12361 cacgaaagac gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata
    12421 tcccagtaat tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat
    12481 taggtgcttt ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa
    12541 gatatgtcga agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg
    12601 acaaaaaaga aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt
    12661 acttttcatt ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac
    12721 catgtttgaa cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg
    12781 ttcagtcatc cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa
    12841 tatcgcaccc ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc
    12901 gatccagaga aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga
    12961 agctgttatc catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa
    13021 tctgcctaat gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat
    13081 catttcagta ctagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca
    13141 tgtcgagata cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa
    13201 atatcattta aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt
    13261 gaatgctgta agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat
    13321 tgcacgtccc gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac
    13381 agattcaaaa gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta
    13441 cttagaagtt ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa
    13501 ggaaatagaa atgccagatc aaacgattag cggattgttt aattacttta acaaataaat
    13561 atttattccg aaaagtgaaa aaacatataa aaaaacacat aaaactaaaa aattatcaaa
    13621 gaatcctggt gcaactgaaa gaatttgcaa cgggattctt tcaacttatt tgttattttt
    13681 ttctaaaaaa tattaaagat tgttaaattt cactatttca tttcaaattc cattgcctaa
    13741 tcattaggaa aagtctatca tgaactcgta aatagaatat agtaataacc ttgttaggtg
    13801 aggctcctat acaaacatag gctgttgccc agaaacgtcg aaagtcgcca atgggtaaaa
    13861 cagaaactgt tggattaagg ctttttccaa tagctaagaa tgttttcttt acgttgtata
    13921 gtgctaaaac tcggacgatg agatgatgct aggtcagaat tcttgttcga aaaggtttct
    13981 gaccttttct ttatattttt gtgactgact tatttgatga gacaaactcg ctcctcacct
    14041 gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt ggtggatttt
    14101 gggtccttat ttgactttgt ccatgatcgg tcttttagaa gtatacagtg ccagttctta
    14161 tcgtttattg caagccgatg aaaatacaaa aagtctatta ttgcgtcaac taattttcat
    14221 atttttgagt tggagcgtga tcttcttagc tcgttcagtc aaactacact atttacttca
    14281 ccctaaaata gcaggatacg gtttagcctt atcgattttc tttttagtat tagtaagaat
    14341 agggatattc ggtgtcactg tcaacggcgc acaacgttgg atctctctgt ttggcattca
    14401 attccagcct tctgaactgg caaatctttt tttgattttt tatttaagct ggttttttcg
    14461 tgacggaaat agtagcccaa aagatctaaa aaaaccattc ctgattacag taggtataac
    14521 ttttctgatt ttatttcagc caaagattgc tggagcattg atgatccttt cgattgcgtg
    14581 ggtcatattt tgggcagcgg cggttccatt taaaaaaggg atctatctaa tcgttacttt
    14641 ttctgcattg ctgattggag cagcaggcgg ggtattatat ttaggaaata aaggttggct
    14701 tccacagatg tttaatcatg cttatgaaag gatcgcgact ttaagagatt cttttatcga
    14761 tagtcatggt gcgggctatc aaatgaccca ttcgttttat gcactatata atggtggcat
    14821 atttgggcgg ggattaggaa atagtatcac aaaaaaaggg tatcttccag agacagaaac
    14881 agattttatt ttttcgatta ttacagaaga gcttgggcta atcggtgcat tgtgtgtgct
    14941 tttcttattg ttctcattat gcatgagaat cttttgttta agcagtcgct gtaaaaatca
    15001 gcaagctgga ttgtttcttt taggattcgg aactttgcta ttcgttcaga cgataatgaa
    15061 cgtgggaagt atcgcggggc tgatgccaat gactggtgtg ccattgccat ttgttagcta
    15121 cggaggaaca agttatctga tcctttcttt aggaatcgga atcacgttga atatttcttc
    15181 aaagatacag gccgaagagt tggcgctcta tcgacctgaa aaacaataaa gagaaactga
    15241 gaaactctca gtttgaatta tcataaagta caatctttta tttttcaagc gagaggttct
    15301 tttagaaaga ggaattcgca atgaaagtat ctcaaaaaat catattatct attttgttac
    15361 tgcttacatt agtagttggc tatttttcga ttgagtttat ccatggattt tcgtctgcaa
    15421 aacagagttc aactgtgaaa aaggtagagc cgaaaagtat ccctaccaca ctaaatgtgg
    15481 ctttgattgg ttcagatgct cgttcgaaag acgaaaacgg tcgctcggat tcacttctgg
    15541 ttgcacaata caaccagaaa acacaacaag caaaactgat ctctatcatg agagattcat
    15601 atgtcgatat tccaggttac ggaatgaata agataaatgc agcttactct tatgggggag
    15661 ttgatttatt aaatcaaaca ttaaaggaaa atttcaaatt tgaagctccc tattatgcaa
    15721 gtatcacatt tcaagatttt atcgattgcg ttaatgaact gtttcctggt ggagtcaaga
    15781 tcgatgcaga aaaatctcta gatttagatg gcgtatatat aaacaaagga gagcaagtga
    15841 tggatggtaa tactttactc cagtatgctc gatttcggga agacaaagaa ggagacttcg
    15901 ggaggatcag aaggcagcag caagtgatca aagctatctc tcagcagctg aaagacgtaa
    15961 cttcaatatt caagctaccc aaagcggttg ggaaattact tggtagtatc caaacgaacc
    16021 ttccagaaag tgtgcttctg gattgtggta tggatttcct caaagacata tgttagaata
    16081 aacaggtata aatagtgaaa taaaggaatg acaagcaaga gaaggaggaa aaaatgaaaa
    16141 gaagtgacaa gcacggcaaa aatcgaacag gcgcttatat tgccggcgca gtgatcttaa
    16201 tagcagctgc gggtggcggt tatttttact accagcacta tcaagaaacc caagcagtag
    16261 aagctggaga aaagacagtt gagcaatttg tccaagcttt gaacaaggga gattataaca
    16321 aagctgcaga aatgacatca aaaaaggcgg caaataaaag tgcattatct gaaaaagaga
    16381 tcttagataa ataccagaat atatacggtg ctgctgatgt caaaggactt cagatatcga
    16441 atctaaaagt agataaaaaa gacgattcta cttacagttt ttcatataaa gcaaagatga
    16501 atacctcatt aggtgaattg aaagatcttt cttataaagg aacattagac agaaatgatg
    16561 gtcaaaccac gatcaactgg caacccaact tggtttttcc agaaatggaa ggaaatgaca
    16621 aagtaagcct gaccacacaa gaagcagcaa gagggaatat tatagatcga aatggtgaac
    16681 cattagcaac aacaggcaaa ctaaaacaat taggagtcgt tccaagtaaa cttggagatg
    16741 gaggcgaaaa aacagccaat atcaaagcga ttgcttcctc cttcgactta acagaagatg
    16801 ctatcaatca ggcgatttca caaagctggg tacaacccga ttactttgtc ccattgaaaa
    16861 tcattgatgg agcaacgcca gaacttccag ctggagctac cattcaagaa gtagacggca
    16921 gatattatcc tttgggtgaa gcagctgctc agctgattgg ttacgtggga gatatcacag
    16981 cagaagatat tgataagaat ccagaattaa gcagtaatgg aaaaatcggt cgctcaggtt
    17041 tggagatggc ttttgataag gatcttcgtg ggacaacagg tgggaaatta agcattacag
    17101 atgcagacgg tgtagagaaa aaagttctga tcgaacatga agttcaaaat ggcaaagata
    17161 tcaaattgac aatcgatgcg aaagcacaaa aaacagcttt tgacagtcta ggaggaaaag
    17221 ctggatcaac tgttgcgaca acgccaaaaa ccggtgatct tcttgcgctt gctagctctc
    17281 caagctatga tccaaacaaa atgacaaacg ggatctcaca agaagattac aaagcttatg
    17341 aagaaaatcc tgaacatcca ttcatcagcc gatttgcgac aggttatgct cctggatcta
    17401 cgtttaaaat gattacagca gcaatcggtc tcgacaacgg cactatcgat ccaaatgaag
    17461 tgttgacgat caacgggctt aaatggcaaa aagacagttc ttggggatct tatcaagcaa
    17521 cgcgtgtcag tagtgatgta tcacaagtag acttaaaaac tgctttgatc tattccgata
    17581 atatatatgc ggcacaagaa acgttgaaaa tgggtgagaa aaaatttcgt acaggcttag
    17641 ataaattcat ttttggtgaa gaccttgatt tgccaattag tatgaatcca gcacaaattt
    17701 ctaatgaaga tagctttaac tcagatatct tgctagctga tactggatat ggacagggcg
    17761 aacttctaat tactcctatc cagcaagcag caatgtattc tgtttttacc aacaatggca
    17821 cacttgtcta tcctaaattg attgcagata aagagacaaa agataagaag aatgtcatca
    17881 gcgaaacagc agtacaaacg atcgtgccag atctgagaga agttgtgcaa gatgtaaatg
    17941 gtacagcaca ttctctttct gctttaggga ttccgttggc agcgaaaact ggtacagcgg
    18001 aaatcaaaga aaaacaggat gtaaaggggc aagagaacag tttcttgttt gctttcaatc
    18061 ttgataacca aggttatatg atggtcagca tgttggaaaa taaagaagat gatgattcag
    18121 caaccaaacg agcatcggaa ctattgcaat acctcaacca aaattatcaa taaaaaattg
    18181 ctttttaagt acagtaaaag gactgtggcg ttatcgtcac agtccttttt tcaaataaac
    18241 ggttttcacg atgtctcttt cacaacttct ttttgattta ctcacacttc ataaactttt
    18301 tttgtaaaat atttgagaac aagtggagaa ataagtgtac ttaataaaat cacgataact
    18361 aaaggtgaat agtactgtgg ttcgatcaat tgactttgct gtccaatttg taaaatgatc
    18421 aaagccattt ctccacgcga aatcatacca gcaccgacca tcaatgaact atgttgagaa
    18481 aaaccggcaa tctgagcacc aaaataacct ccaagcaatt tggtcaaaat cgctacaagc
    18541 gtcaatatag aaataaagag tagttgttca ctaaagtcag aaaaatcaac ttctaatcct
    18601 acactgacaa aaaatactgg tataaatact gcatatccta aagcctctac attgtggtat
    18661 acttcttctt taacctttgt ctgactgaca gctatcccag caaaaaaagc accaatgaca
    18721 gagctaagcc caacaagatc cgctacgtaa gacattccta aacaaataac tagcgatacg
    18781 atgataatag aagaatttgc gtacactttt tctgcaaatg aaagtaggaa aggcgcaatc
    18841 catttgacta ataagaaaat aaaaacaaaa taaattagct gttcaatcaa aacaagcggt
    18901 aaagaagcat tgctagaagc ttcccccgtt agaaaagata ggctgaaact tagaactaaa
    18961 acgactaaaa tgtcatctac cacagaagca cccaaaattg ttgatccttc ttttgtattc
    19021 actacgttta gttcttttaa tacttctact gaaatactga cagaagttgc cgcaagaata
    19081 atcccaaaga aaatcgcttc atttcccgaa acttggaatg ctttgcctgt caaccagcca
    19141 aaaacaaccg ggaagatgat cccaagcaaa gcaacgaaca taccaggtcg gaaatatttt
    19201 ttcaaaagag ataaatcact ttccagacca gctaaaaaca ttaataagat cacaccaatc
    19261 tccgaaaagt catggactag aatatcaggt ttgacccaat tcaatccagc ttggcctagc
    19321 aaaacaccaa caagtaattg tccaatcact gctgggatac ctaatctccg cgagatatgt
    19381 gagccaatcg ttgttgaaat caatatcagg cataaaatac caataaattc cattctcatc
    19441 attcctttgt ataaaatata gcccgaactt agcataacat acattattga aaaaatagga
    19501 aaatatactc acaaaaaaac caacaatcca aaagattttt ttggattgtt ggtttttata
    19561 cggtctattt taataactaa aaatgattaa cgtatagtca tttttcttat tctgatgaaa
    19621 ttgataatct cctggatgcc catgaaaatc aaaataaagc cgaatatttg gaatagaagc
    19681 atcaaacttt tgaatggatt gaacaataag acgaatccgc caatcaacaa cagaatacta
    19741 ctataaacac tccaactttt agcttttgta ttcatgctga agaacaactg gaacaagctg
    19801 ttgatgataa cagctaatcc aagaagaatc ggcaggatag aaacgattgc aggtgcaaaa
    19861 gcatagacga tcagagctaa gatcaggaaa aaaagaccac tgaacagacc gatactccaa
    19921 gaacctgttt tccttttaaa acggtaagcc tcaaaaagat tcaacaaccc aagcaaaaca
    19981 agataggcgg tgatcagata accgataaaa tgaaaaacag cagaagggtt tagcacaatc
    20041 gcaatcccag aaataatata aacagcagct cttaataagg cgtatttttg aatattttgt
    20101 ttaatcgtct caaacatata agaaaacctc ctttgactta ttttaacgtg aactagtcat
    20161 tttaccaaat taaatgttac aaaatgagcg aaggagaaga atatctctgg aaaaatcaac
    20221 ttctgaggat cttttccatt tttttgcctt ttgctagctc atcgatcatt ttatctaaat
    20281 aacgaatttc tttcatcaag ttatcctcga tgtcttctac tcgtacacca caaacaacac
    20341 ctgtaatcaa ggaacgtgaa ggattgggga caggggcttc cgcaaaaaat tgttcgaaag
    20401 tcacttgttt ttgtagttga tattctagtt ctgtttggga ataacccgtc agccaacaaa
    20461 tgatttcatc tacttcctct tttatacgat cttttctctc cgcttttgct acatacatag
    20521 gataaacgct tgcaaaactt gtcgtaaaaa tacgattttg cgccataatc agtcaccttt
    20581 ctttttttca aattatttac tacttatagt atataagaaa aagagaagaa aaagcgatta
    20641 ttcaaaaaaa caaaaaggat tgtgacaaaa ttcttgtcac catccttttt tccgctctct
    20701 gactttttac aatcttcata gctcaagtgc gcatcagata atcgaatgct cctaaagctg
    20761 cagtagctcc tgaacccatt gaaacaatga tttgtttgta tggactatct gtacaatcac
    20821 cagctgcgaa aacgccgggg atattcgttg cgccattttt ctcggtcact atttcatttc
    20881 ttggattcat ttcgatcgtt ccatctagcc aatccgtatt agggagtaag ccgactagaa
    20941 tgaatactcc tgaaacagct agtgtatgca tctccatcgt acggcgatcg atataggaga
    21001 gagattctac ctgattcgta ccgctgattt ctttcgtcgc tgcatgtttg ataacagtga
    21061 cattcgcaag agagactagt ttatcctgta agatttgatc tgcttttaat tctggtaaga
    21121 attctagaac atatacatgt tttgctaacc cagccaaatc gattgctgct tcaacaccag
    21181 aattaccacc accgataaca gcaatctctt ttcctttaaa taaagggccg tcacagtgtg
    21241 ggcaataagc aattccttta tttttaaact ctttttcgcc tggtacattg atagaacgcc
    21301 agtgcgcacc tgtcgcgatg atggcggttt ttgctttaag tgatataccg ttagctaagc
    21361 caatattaat aaaatgttgc tctttattta aggaaacggc ctgttgattc atcataatgt
    21421 cgatagggta actacgcaca tgactttcta cttgctgcat caattttgga ccttctgtgt
    21481 aagaggtacc aatcatattt tcaatcccta gtgtttcaac gacttgtccg ccgaaagaat
    21541 cagcgacaag tcctgtacgg attcctttac gtgctgcata gatagctgca ctactagctg
    21601 ctggtccacc gccaatcact aatacatcat aaggttcttt ttcaaacaac tcttcttcaa
    21661 tcaaagggcc agtgattttt tcaacaattt gttcgatcgt catgcgtccg ctactaaatt
    21721 cttctccatt taaaaacaca gttggcactg ccatgatttt tttatcatcg atttcttttt
    21781 ggaacatccc accttcaatc atcgtatgag taatatttgg atttaataca gctaatatat
    21841 tcaatgcctg tacaacgtcg gggcaattat gacaagtcaa gctaacataa ctgtcaaaat
    21901 gcaatgtttg tgttattttt ttgattttaa gactcaaaga ttcactgatt ttcggtgccc
    21961 gtccactaat ttgtaacata gccagtaaaa aagaggaaaa ttcatgaccg agggggatac
    22021 cagcaaaaac gattccactt tctttgccaa ctggattgat agaaaaacta ggtgtgcgca
    22081 tcgaatctct tttttcccaa gagattttag gagacatttc tgctatttcc ttcgtaaatt
    22141 cagctagttt ttgactattt tcactattat ccaaactaac agaaaaaaca ataggagatt
    22201 caatcagtgc taaatattgt tcgagatttt gtttagtttc tttgtctaac atatccacca
    22261 cctaaatctt tccaacaaga tcaaaacttg gtttcaatgt ttcgccactt tctttccatt
    22321 ttgccggaca aacttcacct gggtgggtac gtacatattg agcagcgcga atcttatcaa
    22381 tcagtgcact agcgtcacga ccgattccat ctgcattgat ttccatagct tgaacgattc
    22441 cgtctggatc aataataaat gttcctcgct gagctaatcc ttgctcttca tccaaaacat
    22501 caaaaatacg agaaatctga tgagacggat caccgatcat cacgtattcg attttaccga
    22561 tagcatctga tgtatcatgc catgctttgt gagtaaagtg ggtatcggta gagcaagaaa
    22621 agacttcaac acctaaagat ttcaatgtac tgtattgttc ttgtaagtct tctaattcag
    22681 taggacaaac aaaagtaaaa tcagctggat agaagcaaag aatactccag ttccccataa
    22741 gatctttttc actgacttct aaaaattctc cttgatgata agcatgtgca ttaaattctt
    22801 cgattttttt cccaatcaat gacatagtaa aacctccaat aataattatt tatttataat
    22861 tattgtaatt tagttggagt tgcaaagtca atggatattt tgttatttaa taaagattac
    22921 tcaaaaacga aggttaatta taaaaataga agaggtcgca agaaaatgct actgctggaa
    22981 atactcggga taacgttgag taacagtttc ctgttctttc agcatcatgt gtaagtgcaa
    23041 tcccgtttga aatcgagctt cttctggttt tttatcaact aaagccttga gtaaatcata
    23101 atgttgttcc atgatcgttt cccatggaag cccctgaatg gtcactcgta accagcggaa
    23161 acgttctaaa tgggtattat ggtcatctaa ccagttccaa acttcttttc ttcctgcaat
    23221 ttcgaaacaa gcttcatgaa ataaattatc tgcacggaaa aaagcgcgtt cgtcatttat
    23281 tttgactgct ttttcttgtt cttctaaaat cgtttctagc actattcgtg tttgtgtgtc
    23341 caatttggaa caacattcaa tcatgatttg ctcttccaac tgttctcgga caaagcgagc
    23401 attttctgca gaaacaagat taattttgga aacatacgta ccactttgag gtatcgtata
    23461 cacgagttct tgctgacgta gttgaatcaa tgcttcgcga atgggagtcc ggccgatagc
    23521 aagatgttcc tccagtgcct tttcagatat tttccttcca ggttctaagt cagcatagat
    23581 aatttgttta cgaatcgctt gataagcttg tgattgcaag ctgcttttca tctttattcc
    23641 tccaaaatca tttctatgaa cgtagtataa cagaagaggt ctgaaaaaca atctggtatg
    23701 tcacattaac tagtaaattt gtataaatag taaaattttg gttgacatag cgatttttat
    23761 tgcataaaat tcacttgaat acaaaaacta atatgttagt ttaggagggg aaaaatgatt
    23821 gatttaaaca actggaggaa ctttatccct gaattatctc aaaaagaagt agaaatacct
    23881 cattttgacc gtgcatcatt aagagaaaat ggacaaaaaa atcctgtctg gatccatttc
    23941 ggtggcggaa atctttatcg aggatttcat gcagaaatcg ctcaacgttt agcggatcta
    24001 ggcgaattgt ccactggtgt cattgtttgt gaaacattcg atgaagaaat aattgaaagg
    24061 gcttaccaag attttgatga tttgattctt gaagtggtca tgccaagcag tggagagttc
    24121 gtcaaacgta tattagctgc gacagctgac agtctgtatt gtcatccaga ttgttctgaa
    24181 cactttgata agatgattcg acactttgaa aatcctagtt tacaattagt tacttttgct
    24241 attactgaaa aaggttatcg attgaaagat acaaataata aatatatagg accgattgca
    24301 gtcgatataa aaaatggtcc aaagaagcca tcacatacaa tgagtattct tgctgtgcta
    24361 ctttataaac gttttttatc tggagcttgg ccgattgcac ttgtctctac agataatttt
    24421 tcacaaaacg gtagtcaatt acgtacttct ttgctaacga tcgcaaaaga atggcaaaag
    24481 gaaggttatg tacaaaatac atttatccaa tacttatcga atgaagaaat tgttgcgttt
    24541 ccatggacga tgattgatcg tatcaccccg aatccttcct tgtcagttag caaaatgttg
    24601 gcaaaagagg gcttttcaaa tatgtcgatt ttccaaaccg caaaaggaaa taatattgct
    24661 ccatttgtca atacagagga catccattat ttaatcatgg aagatcattt tcccaatgga
    24721 cgtccgaaat tcgaaaaagc tggggtattc ttgactgatc gtgcaacggt agataaagca
    24781 gatacgatga aggtgacgac ttgccttaat cctttgcata cgtctttagc tattatgggt
    24841 tgtttactgg ggtataggag catagctgag gagatgaaag atccagattt atcgctgcta
    24901 gtttctgaaa tcgggtacaa tgaaggcctc ccagttgtgg agtctccagg tatcattgat
    24961 ccaaagatct ttctggaaga agtaattact gtgcgctttt ctaatccaaa tattcctaat
    25021 atgcctcagc gaatcgccac ggatacctct caaaaggtgg cgattcgtta tggtgagacg
    25081 attaaaaaat atatggaaat agaagttaaa gacccgcaga cacttgtatt cataccattg
    25141 actctcgctg cttggcttcg ataccttatt ggcattgatg atcaaggaaa accattcatc
    25201 ccgagtccag acccactatt gacagacctt cagcataagc tttccgatat tcaactgggt
    25261 acgcaagata catcacatat tcatgagaaa atggctccta ttttgtcaaa taaagaaatt
    25321 tttggtgtag atctttacca agcagagctt ggcagaaaaa tagaagaaat gttcatcaag
    25381 atggtagcag gaccaaatgc cgtacgaaaa acattacagg attacttaca agaacatgga
    25441 ggaaatttat catggaaatg acttttagat ggtacggatc acaggaagaa aagattacct
    25501 tagatgaaat cagacagatt cctgcggtca aagggattgt aggcacattg atggatat
//
LOCUS       DACEPW010000020        25516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEPW010000020 DACEPW010000000
VERSION     DACEPW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.020"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="IW819_000629"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="IW819_000629"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1243757.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="IW819_000630"
     CDS             complement(860..1678)
                     /locus_tag="IW819_000630"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1243758.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IW819_000631"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IW819_000631"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1243759.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="IW819_000632"
     CDS             complement(2967..3923)
                     /locus_tag="IW819_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1243760.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="IW819_000633"
     CDS             complement(3923..5056)
                     /locus_tag="IW819_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1243761.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="IW819_000634"
     CDS             complement(5049..6611)
                     /locus_tag="IW819_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1243762.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="IW819_000635"
     CDS             complement(6823..7905)
                     /locus_tag="IW819_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1243763.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="IW819_000636"
     CDS             complement(7971..8369)
                     /locus_tag="IW819_000636"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1243764.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IW819_000637"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IW819_000637"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1243765.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="IW819_000638"
     CDS             complement(9098..10399)
                     /locus_tag="IW819_000638"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1243766.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="IW819_000639"
     CDS             complement(10540..11556)
                     /locus_tag="IW819_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1243767.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="IW819_000640"
     CDS             complement(11864..12469)
                     /locus_tag="IW819_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1243768.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /locus_tag="IW819_000641"
     CDS             12665..13588
                     /locus_tag="IW819_000641"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1243769.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="IW819_000642"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="IW819_000642"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1243770.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15703..17334)
                     /locus_tag="IW819_000643"
     CDS             complement(15703..17334)
                     /locus_tag="IW819_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1243771.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17348..19021)
                     /locus_tag="IW819_000644"
     CDS             complement(17348..19021)
                     /locus_tag="IW819_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1243772.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19034..19351)
                     /locus_tag="IW819_000645"
     CDS             complement(19034..19351)
                     /locus_tag="IW819_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1243773.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IW819_000646"
     CDS             complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IW819_000646"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1243774.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21013..21798
                     /locus_tag="IW819_000647"
     CDS             21013..21798
                     /locus_tag="IW819_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1243775.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21801..22493
                     /locus_tag="IW819_000648"
     CDS             21801..22493
                     /locus_tag="IW819_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1243776.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22767..23138)
                     /locus_tag="IW819_000649"
     CDS             complement(22767..23138)
                     /locus_tag="IW819_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1243777.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(23538..23768)
                     /locus_tag="IW819_000650"
     CDS             complement(23538..23768)
                     /locus_tag="IW819_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243778.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(23862..24200)
                     /locus_tag="IW819_000651"
     CDS             complement(23862..24200)
                     /locus_tag="IW819_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243779.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(25128..25244)
                     /locus_tag="IW819_000652"
     CDS             complement(25128..25244)
                     /locus_tag="IW819_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1243780.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         7435 a         5604 c         4500 g         7977 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aacgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcactg attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggcccctagt ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagc agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatcca ttaggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aaggatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtgcttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaataat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgcc tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcga tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat aaagagccaa cataatatgg agggggaatc
    15241 cctataaatc aacattcttc aatagctaaa atgcttgatt ttcattaaaa ataagatggt
    15301 tccctaccaa aagagactat aatataagtt aatttgtttc attcttatca taaaaagaag
    15361 ttctcgaaaa acgaactgtt ttccgagaac ttttttctat ccatctaagt gctcaaatca
    15421 tattcacgac ggaattctaa ggttaccctt tcttaaacaa actttatgtt atctgcacaa
    15481 ctttttgcaa ttttttcatt gcttcttacg aaatcgttga actctcttta gcataataca
    15541 aagtgtagat gaagtctggt gctttactcg ttttggtaaa aatgactgga aatcgtttct
    15601 ggttattttt ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc taacgatgtg
    15661 gctgaaggga acctccgatc ttgaatcgaa acgatccgca gtctattgtt tcaccaatga
    15721 aacaaaatca acgttcgtcc aagcatttcc tgaagccttg ctataaaccc caatttcaca
    15781 aacacctgtt gtaattggaa tgtctttgat tgtaattttt gtccattcac ttgttttaac
    15841 aaccgcagta tttagttccg ctccaccata atttttgata tataactgat tagtatcaaa
    15901 ctctccccca gacattacat aggcactcaa tgtgtaagtt cctttgctta tgccactaac
    15961 attttgataa gctgatgcct cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc
    16021 aaaaaaagtg cccgcttccg ttttgatcgt acccttagcg gttccttgtt gaaaccaggt
    16081 ttgccaattg gaaggactgg tcgttggtcc atcgctttcg aaacttgcat ttttaactag
    16141 atttatttgc gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa
    16201 atcaaacatc gcttggttgt cccattcgtt tcccatagtg gattgaacac tgagataatt
    16261 catacctgct tgtgttgccc aactcacatt tccgttgtac caaagaggtt cccaatagaa
    16321 gaagcccgcc ccgcgattat tcggtactgc ctgaatccga tcggcaagat ccagcaaata
    16381 ctctgcttgt cctgtcggtg ttggttcata tccccccgca gctgcttcgg cttcaccaaa
    16441 agcatttggc atagtatcgg cattcgctgt tgtatgccca taagctgttt caactacgat
    16501 cacttcttta ccataccgtt ggctgatcgc attcatattg gtttgaagtt cattcattga
    16561 accgtgccaa tacggataat acgacactcc aattaaatca aatgacacac ctcgactcgt
    16621 gatttcatca aaccaccaag taaatgctcc tgtatcgcca ccatcagcta agtggagcat
    16681 tactggagtt gttttaggtt gagtgtctcg aacagcttgg ataccagact tcaaaaaagc
    16741 agccaatcga tcaaattctc ccccgccttc tccccaagac ttgccataag gccagagcat
    16801 tccgctattc agttcattgc cgatctgaac catatcagga taaacgtcca gctgcttcat
    16861 ttccgacaga acgttctttg tatagtcgta caatgccgta ttcatttctt caaaggtcag
    16921 accttgccat gctttaggaa gattctgttt cccaggatca acccaaaaat cactgtagtg
    16981 aaaatccagc agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt
    17041 ttccagatca tttgtccctg ctccataact ttctccatta ctattataag gatcctgcca
    17101 caaacgcagt cgaacatagt tgacaccatt attttttaaa atctctaagg catcttttga
    17161 aatatcgtct tcataatatt ttgcaccaga tttttccata tctgacagga tcgaaatatc
    17221 tgctccgcga ataaattcgc tggcagatgc ttcaacagtt tttgaaacag cccccaaagc
    17281 ccctaacaca gctgcaaata aaacagcttt acgcaaccaa attcccattt tcattatggc
    17341 tcctccttta ttgaaattgg ttcagaataa actgaacagc tttttcagga tctctcgtta
    17401 atccgatata ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt
    17461 ctttgatatc atcatagtaa gaattgactt gcggtgctaa gaccaccata tcaaaatcct
    17521 gcagcatttc gtaatgagaa ccataagcac ccgccattgc actgatcggt gctttcgatt
    17581 cagatgcacc tttttttaaa gcattagcta acatcgcact tgtccctgcg ccagcacaaa
    17641 gtaccaacac attgatttct ttatttgcat cgctgataac ttgactcgct gccacaggta
    17701 cttcctcacg tacatcggca gaagcacctt cctcggttgc gatcacctct gctttttgtg
    17761 cttctttctc tactaagaca gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt
    17821 caacgacgat tagaaccgct gctagaacaa aagctaagat acccatgccc gttcctagaa
    17881 ccaaaccgat cggtgctgga gttgcccaag gcaaaacgta cataaagctg ttcatgccca
    17941 atacatcgac aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa
    18001 taaaaaaata agggttcaaa atcatgggtg ccgcaaatag caatggttcg ttgacagcga
    18061 aacttactgg aataaaggaa gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga
    18121 gtaggaagat aaaaggaaca accaatgttg ccccagttcc tcccatagtt cctacaaaat
    18181 tccctaaacc caccgtcagt actttagaag cttgttcacc ggaattgaac aattgcagat
    18241 tggtttccac attggcatag ataattgccg caattgccgg ctccacgatc gacggtccat
    18301 gaacaccgac aaaccaaaac agcgccatcg ctccccagat aatagcgatt cctaagtacc
    18361 cgtcagctgc tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca aatgagattt
    18421 ggaagatttg tcgaatgcct aaatcaatta aaacggccgc aaatactgaa aaagagaaag
    18481 gaaaaatatc tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc attttaatgg
    18541 taatatcttt cagcacacaa aacttatata aattaactgt taaaaatgca gcgacaaagg
    18601 aagccagtag tccctttgtc cccatatatt ccgttgaaaa tccaccttca atcggagaga
    18661 cgcttaacaa caggaaactt accacagagg ccagcatcac agaggtggtg ttgattactt
    18721 tgtttttcgg taagcgacga ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa
    18781 cgagtaaccc tacaacaccc atcgaataat tatagacttt ccatagccat gtggagacag
    18841 attcaggcca ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg
    18901 aaaataaaat agctggcatc gctgttaaaa atccgtcacg aacggctcct aaataactat
    18961 tacgtgagat tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg attccatcca
    19021 tagcgttctt ctcctatcct tgattctgtc tgtacagtac aataaagtct tgaatcagat
    19081 ctctcagcaa taaagtggtc attaaatgat cctgcccatg gataaaaatg aatcccatct
    19141 ccatctcttt cccagatgct tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg
    19201 ttaaattttc gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta
    19261 cttctttcaa taatttcaat aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa
    19321 aaccaagcat ttgtaattcc tctttattca tcttttcttc ctccattatc acttattaaa
    19381 taatcgtttt ggtttcactg accaacttgt accagtaagc tgattctttt gggtaacgct
    19441 tctgggtttc aaaatcaaca taaaacaagc catacctttt gttataccca tttgtccatg
    19501 agaaaagatc cataagcgac cacaaaaaat accccttcac atttaccccg gctgttatcg
    19561 ccttgcttaa ggattccaaa tagactctca aatagtcgat tctcggctga tccataataa
    19621 tgccatcttc aaactgatct ttatacccca tcccgttctc agtgatatag attttgttgt
    19681 aatgtgggta atcgctttta attcgcagca acaagtcata tagaccttct ggataaatca
    19741 gccagtccca atcagttcga ggaattcctt ctttataaat acgttctccg attcccttca
    19801 ctttataaac agaggtccct ttttcacccg taccgttatg atggattgca ttttctccat
    19861 cgtaagcttt gacaaaatgg cattggtagt gattgatccc taagtagtca ttacgcgtgg
    19921 atgctttttt caattcaacg aaatcttctt caggaaaatg atagctggcc tgatttgctt
    19981 cacatatctc atcaagagca gtcaaggttt cagttgaata atagcctaaa taggtagcat
    20041 ctaataagaa acggatcgac aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg
    20101 cgtctgttgc cgcatatttc gtctctaatg agtgaactac accaatttca cccggaagtt
    20161 cgttttcttt gaaatagttc actacacggg catgagcaac catcatatta tgaagacagg
    20221 cgacgatctt tgtaaaatca tatttgattc ctggtgggaa aacacctaac aaatattgat
    20281 tggttgcaac tggatagatt tcattaaagg tactccacac cttgacttct ttgaattcat
    20341 gaaaacaaaa aatggcatag gaaacaaatg cttctattgt ttctcgattc aaaaaatctc
    20401 catggtcaaa caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt
    20461 ttcttttctt acattcttcg aacacacgat gataaaaagc gattccttct ggatttggtt
    20521 tcccagctcc atcaggaaaa atgcggctcc aagcaatgga taatcttaga ccatttacac
    20581 caaaacgttc acacaattca atgtcctttg catattgatg ataaaaatca cttgccggat
    20641 cgggactgaa tcgcccttgt tcctctaaaa agtcatccca agcaacagcg cctttgccac
    20701 cttccttagt ggctccttca acctgatagg ctgctgtggc tccgccaaaa ataaaatctt
    20761 ctggtaattt aagcatgtac atctctcctc aaatctataa tcagttttcg ctctttctaa
    20821 ctgattgcat tgtaaacgca tccaaaacat ttgtcaacaa aaacaaacac aaataatcat
    20881 aaaaaccaga aaacagcaat aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt
    20941 tacttttgtt cgaattagat tgactgttgt ccgtttatat gatagattat ccttaaatta
    21001 gaggtgaaga caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa
    21061 aaaggaatcc ttaccgttaa tgaaatcatc aatcaattag atgtttctga tatgaccgtt
    21121 cgcagagatt tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct
    21181 caaagtcttt cctacacttt agatcaagaa ctttctcaca ctgaaaaagc cactctgcag
    21241 attcaagaaa aaaaagaaat tgctgccaca gcagcctcct tgattcacga gggagaaacg
    21301 attttcttag gaccaggtac aacaatcgaa ctattggcga cgcatttact gaacaagaaa
    21361 attcggatca ttacaaataa ttatccagtt tttgatattc ttgctcaagc agatggacca
    21421 gaaattctac tgattggcgg tgatttccgg aaaaataccg gcgcatttgt cggaccgatc
    21481 accaacgaca atttaagaag actaaagttt acaaaagcat ttatcagttc aaatggcgtt
    21541 cacaacgaag cgatctctac ttacagtacc gaagaaggag aagcacagca gatcgctcta
    21601 aataattccc gtaagaaata tctactcgtt gataataaaa aattcaatcg agatgatttt
    21661 tatgtgtttt ataatttgca tgatttcgat ctgctcatta cggatcgcca gatctcgcgg
    21721 gaggttcaag agcactatgc acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat
    21781 accactatag gagcgtaact atgaaaggga ttatatttga tttcaacgga acaatgtttt
    21841 tggactctca tctgcatgaa gctgcttggc tgcatatgat acatgatcat acaaaagatg
    21901 gtttatcaga cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg gagattttga
    21961 aacatttcat ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag
    22021 aaagctatta tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc
    22081 ttgaagcgac tcttgaccag ctgactgacc aacagattcc attaacgatt gccacggcta
    22141 ctgtcaaaga gaatgttgct ttttattttg atattttcga tttagcccgc tggtttgact
    22201 gggacaagat tgtctatgac gacggctctt tccctgggaa accgcaaccg gatatttttc
    22261 ttaaagcggc agacaaactg agcttagctc cggctgattg tcttgttatc gaagatgctt
    22321 actcaggatt acttgctgcc aaacgtgcgg ggatcggaac aattattgca attgatccat
    22381 tcggtaaaaa caaaacagta tttgaacagg cacagctaaa taaagatgga accatcaaag
    22441 attttactgg tttttggaca gagcacctgt ctgccacatc cgttaattct taaacgattc
    22501 aactaactac caaagaaaag caactgaaat gcacacctcc ttttataaaa aactgccagt
    22561 gaaacgacat gaaagataca ctgctatatc cttagtgaaa tatagcagtg tatctttgac
    22621 tttttcattc ttttaaccat atgacgcgtt catctctatc tataattaac attaaaaata
    22681 gtgttttcac attatttctc aaaccaatct taaaaatcat caggagtact tttttgatat
    22741 acttttatta atgctgggcg taatggttaa aagatttcca ctagttcctc tccaccaagc
    22801 tgagttgaaa aatgcttatc tgtaaacaac atagacaaag aggaaataaa cgtttccgta
    22861 tctttctctg tcccaaggat attaattaat tttcctacta ggtaagtatc attaaactta
    22921 agatctgcct tcacatcaaa agttttgata aaagctttta tcatgcgtac aattttgtct
    22981 ttatcgccat aattgatttc accactcata tcaatatagt cactaatccc tgaagaaata
    23041 atggcaactg cttcttcttt cgccttatca gataattcat ttaatgactg ggattgattg
    23101 atgacttctt gagttttatc tgttacgtaa tctctcatta tcctatcggc atactccgca
    23161 aagtaatctt gaaaaaattc ttctacaggg taaccaactt cgttttccca accaggagaa
    23221 atttcattat cagtacttat taaatctatt aaaaaccaac ccgttctctg agtgttgatt
    23281 ctataatatt tttaatttcc tatataaatc aaaaatatta tagaatcaat aagctaatta
    23341 agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt gtgtagctac
    23401 ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac tgcattaaag
    23461 ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg tcaaaatttt
    23521 tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc ttctacttca
    23581 gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga tccttctctg
    23641 taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc tttccactca
    23701 ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt tcccttttct
    23761 accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta catctagtaa
    23821 attttcatca atttctatat cataaagtat ttcactagtg tctacatcca caatatatac
    23881 tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc gtcactttca
    23941 tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag ctctaagcct
    24001 actgtatttt ttactattga gtcattattt accaccataa cttcgtactg tatataccat
    24061 cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt ctccggaaca
    24121 atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc atgttcctca
    24181 cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa aataaacact
    24241 acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc caatcacaaa
    24301 ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac tacaactatt
    24361 catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga tacttgtaat
    24421 agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa ttccaataca
    24481 caacttatcg actattatct gaaaaccaaa acttattatt ccttgatgtt attctagcaa
    24541 tagtaactgc ttttttattt ttttgcatat tttttcggcg ttacttttta aagttaactt
    24601 tttcgacaaa tagaaagaga tacgaatcac tgattttttt gaagaaaaga aatggagtga
    24661 gctgaacgtt agagatattg ggagctttcg taactgctta ttaaacgata atggttattt
    24721 gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa atatttcgca aaacctttga
    24781 ttatgtagtt acgtttccat tcttgactaa aaatgtttca aaaacgaaca aaggtgttaa
    24841 aaggtaaagt gatcaatact actggtcaaa ataagaggtt gaaagggaat tgaaaaataa
    24901 ctacagataa tacttttata aacgtacgtg ctttgtggtt ctgtagttaa ctatataact
    24961 ggaattaggg aatggtatca atttgaaaag aaagattcta ccaatttatc atatgttaaa
    25021 aatggaaaat caacagcagt tctttcggaa accttacaca aaaaccgatt attgaaaata
    25081 gccgaaaaac gtttttcggc tattttttta attaagtttc cttcttctta ccttttccca
    25141 ctattccgtt tcacatcttt gatgatcttc agcgcacgtt ttcgtgtttt gatatttggg
    25201 cttttcaagc ggcggtatgc acttgagata tctgtttttt ccatatggac gacccacttt
    25261 cttctttgat tgtagtcatg ctaaaaaaca caaatcgaaa caattacgat ttgtgtttta
    25321 aatggtatca aaagttaaaa tgaaagtcaa cttgttaaaa cagtcgatca gactagctga
    25381 ttgactccta tctctggaaa taaattttca ttattacgtt ttagctacct aaaaaaggcc
    25441 ctcttgtccg ttaactttaa tatctattgt tatagctagt ttacctattg aacgtactgt
    25501 gtgttcgata gaacac
//
LOCUS       DACEPW010000021        25484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEPW010000021 DACEPW010000000
VERSION     DACEPW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.021"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW819_000653"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW819_000653"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1243781.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IW819_000654"
     CDS             complement(937..1518)
                     /locus_tag="IW819_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1243782.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW819_000655"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW819_000655"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1243783.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IW819_000656"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IW819_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1243784.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IW819_000657"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IW819_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1243785.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IW819_000658"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IW819_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1243786.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IW819_000659"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IW819_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1243787.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW819_000660"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW819_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1243788.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHRYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IW819_000661"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IW819_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1243789.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IW819_000662"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IW819_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1243790.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IW819_000663"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IW819_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1243791.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IW819_000664"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IW819_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1243792.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IW819_000665"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IW819_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1243793.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IW819_000666"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IW819_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1243794.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IW819_000667"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IW819_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1243795.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IW819_000668"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IW819_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1243796.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IW819_000669"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IW819_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1243797.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW819_000670"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW819_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1243798.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IW819_000671"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IW819_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1243799.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IW819_000672"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IW819_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1243800.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IW819_000673"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IW819_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1243801.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IW819_000674"
     CDS             13972..14157
                     /locus_tag="IW819_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1243802.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IW819_000675"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IW819_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1243803.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IW819_000676"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IW819_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1243804.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IW819_000677"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IW819_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1243805.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IW819_000678"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IW819_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1243806.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IW819_000679"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IW819_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1243807.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IW819_000680"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IW819_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1243808.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IW819_000681"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IW819_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1243809.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IW819_000682"
     CDS             18232..18879
                     /locus_tag="IW819_000682"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1243810.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IW819_000683"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IW819_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1243811.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW819_000684"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW819_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1243812.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IW819_000685"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IW819_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1243813.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IW819_000686"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IW819_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1243814.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IW819_000687"
     CDS             20317..21255
                     /locus_tag="IW819_000687"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1243815.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IW819_000688"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IW819_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1243816.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IW819_000689"
     CDS             21935..22774
                     /locus_tag="IW819_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1243817.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IW819_000690"
     CDS             22750..23619
                     /locus_tag="IW819_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1243818.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IW819_000691"
     CDS             23609..24406
                     /locus_tag="IW819_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1243819.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IW819_000692"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IW819_000692"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1243820.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8278 a         4700 c         5487 g         7019 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcatagatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtaggattt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaatatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaaggaag aggt
//
LOCUS       DACEPW010000022        24969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEPW010000022 DACEPW010000000
VERSION     DACEPW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.022"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            117..947
                     /locus_tag="IW819_000693"
     CDS             117..947
                     /locus_tag="IW819_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1243821.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            964..1815
                     /locus_tag="IW819_000694"
     CDS             964..1815
                     /locus_tag="IW819_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1243822.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            1812..2171
                     /locus_tag="IW819_000695"
     CDS             1812..2171
                     /locus_tag="IW819_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1243823.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVNVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            2186..2347
                     /locus_tag="IW819_000696"
     CDS             2186..2347
                     /locus_tag="IW819_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1243824.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            2344..2649
                     /locus_tag="IW819_000697"
     CDS             2344..2649
                     /locus_tag="IW819_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243825.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2649..3005
                     /locus_tag="IW819_000698"
     CDS             2649..3005
                     /locus_tag="IW819_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1243826.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2965..3210
                     /locus_tag="IW819_000699"
     CDS             2965..3210
                     /locus_tag="IW819_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243827.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            3231..3320
                     /locus_tag="IW819_000700"
                     /pseudo
     CDS             3231..3320
                     /locus_tag="IW819_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            3395..3862
                     /locus_tag="IW819_000701"
     CDS             3395..3862
                     /locus_tag="IW819_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAQ1243828.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            4137..4373
                     /locus_tag="IW819_000702"
     CDS             4137..4373
                     /locus_tag="IW819_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243829.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            4397..4738
                     /locus_tag="IW819_000703"
     CDS             4397..4738
                     /locus_tag="IW819_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243830.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(4731..4910)
                     /locus_tag="IW819_000704"
     CDS             complement(4731..4910)
                     /locus_tag="IW819_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAQ1243831.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            5132..5779
                     /locus_tag="IW819_000705"
     CDS             5132..5779
                     /locus_tag="IW819_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243832.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            6039..6383
                     /locus_tag="IW819_000706"
     CDS             6039..6383
                     /locus_tag="IW819_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1243833.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            6388..6669
                     /locus_tag="IW819_000707"
     CDS             6388..6669
                     /locus_tag="IW819_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243834.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6772..7086
                     /locus_tag="IW819_000708"
     CDS             6772..7086
                     /locus_tag="IW819_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1243835.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            7064..8758
                     /locus_tag="IW819_000709"
     CDS             7064..8758
                     /locus_tag="IW819_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1243836.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8778..9956
                     /locus_tag="IW819_000710"
     CDS             8778..9956
                     /locus_tag="IW819_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1243837.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9919..10605
                     /locus_tag="IW819_000711"
                     /pseudo
     CDS             9919..10605
                     /locus_tag="IW819_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
     gene            10605..11765
                     /locus_tag="IW819_000712"
     CDS             10605..11765
                     /locus_tag="IW819_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1243838.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11775..12650
                     /locus_tag="IW819_000713"
                     /pseudo
     CDS             11775..12650
                     /locus_tag="IW819_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12647..12958
                     /locus_tag="IW819_000714"
     CDS             12647..12958
                     /locus_tag="IW819_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243839.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12948..13301
                     /locus_tag="IW819_000715"
     CDS             12948..13301
                     /locus_tag="IW819_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1243840.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            13291..13692
                     /locus_tag="IW819_000716"
     CDS             13291..13692
                     /locus_tag="IW819_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243841.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13685..14089
                     /locus_tag="IW819_000717"
     CDS             13685..14089
                     /locus_tag="IW819_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243842.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            14101..14706
                     /locus_tag="IW819_000718"
     CDS             14101..14706
                     /locus_tag="IW819_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1243843.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14726..15088
                     /locus_tag="IW819_000719"
     CDS             14726..15088
                     /locus_tag="IW819_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243844.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            15091..15273
                     /locus_tag="IW819_000720"
     CDS             15091..15273
                     /locus_tag="IW819_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243845.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            15290..18709
                     /locus_tag="IW819_000721"
     CDS             15290..18709
                     /locus_tag="IW819_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1243846.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18760..19497
                     /locus_tag="IW819_000722"
     CDS             18760..19497
                     /locus_tag="IW819_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1243847.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            19507..21798
                     /locus_tag="IW819_000723"
     CDS             19507..21798
                     /locus_tag="IW819_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243848.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21822..23912
                     /locus_tag="IW819_000724"
     CDS             21822..23912
                     /locus_tag="IW819_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243849.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            24075..24521
                     /locus_tag="IW819_000725"
     CDS             24075..24521
                     /locus_tag="IW819_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243850.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            24523..24660
                     /locus_tag="IW819_000726"
     CDS             24523..24660
                     /locus_tag="IW819_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1243851.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            24698..>24969
                     /locus_tag="IW819_000727"
     CDS             24698..>24969
                     /locus_tag="IW819_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243852.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT         9036 a         3679 c         5217 g         7037 t
ORIGIN      
        1 atccatcaca cagagaaaca caaaataggt gtgatcaatt tcaaaaataa atatcaaatc
       61 aaagggatca agttaaacca ggaaacaatt aaaaagttaa ggataggagg ataaatttgg
      121 ctgagataag ttggatcaaa cttaaaacta ctatgtttga cgatgaaaaa atacgattaa
      181 tccaagctgt tcctgagtcg gatgccatca tcgttatatg gattcgatta ctagttttag
      241 caggaaagac taacgacgat ggtctgatat atatccagag gaacatgcct tataccgaag
      301 aaatgcttgc tacattgttt ggcaaaaacg taaatacggt tcgcttagcg ctaactacat
      361 tggcaaattt caacatgatt gatctaagca gtgatggact aattgccatc agtaattggg
      421 aaaaacatca aaatatcgag ggtatggata aagtaaggct aaaaaatgct gaaagaaacc
      481 gtaaatacag ggaaagaaag agacaggaac gtctcaaatt ggaaaatgac gttagcgtga
      541 cgtcacgtga cggtacagat aaagatatag aagaagataa agatatagat aaagaagaaa
      601 agaaaggtaa gtattctgac gaacacttac gccttgctaa aaagttgcaa agtaatttaa
      661 ctgaagattt tccaaaagaa atgaacaaag tagatatcga aaaatgggca gacacaatca
      721 ggttgatgga agaaagagat aaagcatcta tagaagcgat tgagtatgtg atcaattggc
      781 tacctacaaa tgaattttgg tttggaaata ttagaagtgc taagaaattg agagaaaaat
      841 ttgagaagct caaattcgaa atcaaagcag acaagaagaa tcataaaaag caaagtcaaa
      901 aactacagta cagcaatcct agtgaatatg acgacttgcc aatttaaaaa ggagatgcat
      961 cacatggaaa gcctagcaaa tgctatggag aaactaataa gaagagtatt agtgcaaagc
     1021 ggaaaatgtc cagaatgtag cgaacctttg tatagttggc gagctaaaaa taaggatggt
     1081 tcagaacgtt gtaaaccaac atgcatgagt tgtggttata aagcgttacg tgtgaaagag
     1141 gatatacaga ccgaacggat atataacgat agcttaaaag cacgagcgtt gagttttttt
     1201 caaaatggtt cggtattaac agataaaact ttgtttaaat gcaaaatgga aaattatcac
     1261 gtagtggatc aagaaacaaa aatcgcttta gaaaaagcaa aaagctatac gaatgaggtt
     1321 ctgctgaacc atcctgcaca tttcattcta tcagggaaat caggaagcgg aaaaagccac
     1381 ttatcaatgg ccacagcttg ggaaatactt gagcgctcaa attatgacaa gaaaatactt
     1441 tttataagct atcaagaatt attagagcaa ataaagtttt cttataacaa tactgaactg
     1501 agaaaagaaa ttgaaggatc gcttatagcc gatattaaaa caactgattt ggtggttttt
     1561 gacgatattg gagctgaatt aggtagtggg gtatcaaata gtaggcagtt tacaaacaac
     1621 acgttaaaca cgctcttaga agccagacag aacaaggcaa cgatcatcac aacaaactta
     1681 tctggtcctg aactaagaga agcctacggt gaaagaattg tttctaggat atttaagaat
     1741 tcagaaggtt atgcgctgaa attccaacaa acagcagaca agcgcataaa accagtgaaa
     1801 ggtagtatcg catgaataaa taccgtaata aaaaaactgt tcatcgaggt atcaagtttg
     1861 attctatcgc agaagcagag tattacgatc tagccttgtg gcaagctgaa gcaaatggct
     1921 ggaaagtaaa acttcaggaa agatttgagc tgatgccgaa atttgaacta gacggaaaga
     1981 agtatcgcaa gatcgagtat attcccgact tcacatttta taaaaacggc aaacttgtca
     2041 aagtcgtaaa tgtcaaagga atgcagacaa aagactttaa gatcaaggca aagttgttct
     2101 gtcatcaata tcaagcgccg ttgatattag ctaaaaaata tcggaatacg ttcaaggaag
     2161 agcgttttta acgaggtggt ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg
     2221 aaacattcag cgtgaaattg acgatctaga acgaacaaag gcagtgatgg tcaatgaaac
     2281 ggcgagaaag gcaatcgatt tgcacataga gaacttaaga agggaaattc gtagattgga
     2341 ggaatgagcg tggataagaa agcgacaatg aaacgaattg ctgaattaac caagtcagaa
     2401 tcttggcagg aagacaaaga aatagttgca gaagtccaaa agctcggtaa atcaatgtgg
     2461 actgaaaagc ctaaacggaa aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt
     2521 ctagtaacag gtacagctga acagttatct gaaattactg gattaagcaa aaacattatc
     2581 tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag gtatgtggag
     2641 gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa atcatgatga
     2701 agattcagaa agacaaaaaa tcaaaagaac gagctgaatt agttaagtta gaaatggctg
     2761 aaacaggagc aggagtgcga agtagcaggc attggaaagc agcagcaaac attgaatttt
     2821 attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg caaacaaatt
     2881 ggagcaagaa acttcatcaa gatcgtttca aatttgtaga gaagtataaa gaaatattag
     2941 aagagtattt gaggaggaca gcaaatgata aaaaaactcg ttcaattcag catggattta
     3001 tatgatatcg aatcaggagc aacactatct gtggaatcgg accatctaat cataaatttt
     3061 ggtggaaagc gccagattat tttgtgggta gttgatgatg tactgtttcc agaaattgtt
     3121 catgatttcg aagaatcaaa agcggttgag tttgaaatag tgaaaaaagt aatggaattg
     3181 attgaaaaat acgaggagga cagcgaatga taccgaagtt tagagcgtgg gatagagaaa
     3241 aagacagaat gaaggcagta gctttggttg aatttgctga ggcgtataaa tcaggagaac
     3301 tcatttcacg tacaaaataa aaaaagtcgg attcctccga ctatgaataa tatttccgac
     3361 ataagtatta tatcataatt gggggaatca gaggatggta cttttcgatg taaagaaata
     3421 tgagacacca agcgcgaaag atgttgatat ggaacgcaca aaacataatg tcgctgtgtt
     3481 tctttcagca tatctatcag ctagatgtag agtagggcaa cctcgagaac caaaagtgac
     3541 agcatcttat tccttggttc caccttctac agctgatcat atatttgaag cagaaagaat
     3601 gctgatcgat aaagaagaag cacaggaaga atttgagtat ctgcataaat tgtttgttcg
     3661 aggctattca gcgatacagc atccgcataa gcctgatgtg actgaaaggc gcaagaagat
     3721 attctatgat cgttatatca atggtctgcc catttatgta actgctcaaa ggaataatac
     3781 tagcgaagaa tcggttaaag tagaatcaaa cagaattatc atccaatttg cttcatcgtt
     3841 agaactggtt gctttcaagt agccagtttt tacacttttt ataccctttt attaccaatt
     3901 tggtttccat tttatacctt ttttatacca atcacttacc tattcaacgt tgtattatga
     3961 tagtgtcgaa agattaggaa acaggatcga caaaataaaa tgtaagggag gaaatctccc
     4021 tcatcgttta attaagcttc gataggcagc agcggaaata ttaagaataa ggatgtgaat
     4081 ttcaactcct tctaaattgt tcttattatc tatcatccgt tgctgtctat taatttatgt
     4141 attggagggt atataaaatg aagatcgctt ccgaaaaaat tgatcaacta acaattttag
     4201 taaaccaaag aatcgaagat attatcaaag aattcgaagg tgaaaagatt aagaacataa
     4261 agcttacttt aatggatcat aagttcgaat taaattcgac aaaaaggaag atcgttgagc
     4321 ttatagaaga agaaatagct ctccagatca atgagcaaca attagctaat taatttatgt
     4381 attggagggg aaatgaatgg aagtcgaaca aataaagtat caaggtatgg atcgggaacg
     4441 gttgattgat attattgagc aacaacgttt agaattgatc caaaaagaag ctgtatgtaa
     4501 agagtacaaa aaacatttgg aacaagtaat tgagtatcat tcagtagaaa agtacagatc
     4561 agtggtgcaa aaaaatagag caacgagtcc tgattctcgt caagatgtat cgattagtat
     4621 caagacgcca gtatttaatt ctgttaaatc agaaaagcta gaaagtatct caactgcttt
     4681 ggtagaaaaa gcagctgaaa tgaactccct agcaaatgaa gttagtcgtc ttatttagtc
     4741 atatcgataa cttgagagtc tttggtgatt ccgtttttta tagaattaat ggtttgttca
     4801 gcggaatatt tagtcaagta tgtttcgcta gttgctacta cttcattgtt attggactta
     4861 ataacgaagt aatattggcc attagttgct tctcttataa caaagtacaa atttttcacc
     4921 acctataatt tatttcagcg gaccactcgc tgataattaa aattataagc tatgtataaa
     4981 atttcacaat atcaatttgt cgctgtggcg gaaagggtag acgcaataaa ggatgaatgg
     5041 gaaaagtcaa tgagtaatta tctgaaattt acaagtcccc tgtttagtta tctaagatta
     5101 tttgataata ttatttctga ggtgatagaa aatggtgggc gtagttaaat ttcttgggtt
     5161 tacatttgat ggatatggta cgttaggcga atgggtaggt attattttga tagtgattac
     5221 aattcaacaa acatcaaagt actactggta ttctaatagt tctaaaattg tatcaagaat
     5281 aactgaagga ataaccaaaa aagatattac tgtcgtcggt aaatatgggt ttactataac
     5341 tttattaaat gaagggaaat ctgaaaccgc aataaagttt tgcgggatta taggtaaata
     5401 taacattttc caacgtttta tcagaaagca ttggtacaaa tatatttaca aataccctgt
     5461 agttaaaaag cttattgaaa aatatgtgga ccacgaaata tcttatctca atccaacaga
     5521 ccttattaat caagaattcc agaatattgg atacttagaa aaaggtctaa aaataccaat
     5581 tgtaaatgaa agtttagtta ctgcaatggt agctaacata aagaacagca agaaattaga
     5641 aaagagatat aaaaaatgta aaaaattgaa attttccttt ttattcttag aatataatgg
     5701 caaagaatat tatcaagata tttatgtttt gcgaggctcg aacttatata aagacattaa
     5761 aaataagatt tcagtatgag attttttaaa attcgtagat cactctttga gtggtctttt
     5821 tattttgcgt aaaggaggca cgacgatgta tagaccacaa tacttagaac agaagcatga
     5881 agtaatcact gtgcaaaacg gtaacggtga gatagtacga aagtatagaa gaccaataaa
     5941 gagcgataca tataaacgga aggaaagcaa tgaagttatt ccattgtatg gcaaaagaat
     6001 agctaagtat taaataagat tgcgaaagga gatgggacat gaccgaggaa ttctatagat
     6061 ggttattaca gttgataaga gaagatcgtt tggttaagtt ctatcagtct cctaaatggc
     6121 gcaggcttag agagaaagcg atgaaacgag atcactatga atgccaagag tgtagaagac
     6181 taggtaagta tcatagagta gagaacgttc atcatataaa ggaagtcaag gatagacctg
     6241 acttagcttt agatttagat aatcttattt gtttatgtgt tgaacatcat aatgaagttc
     6301 atggcagata tcttacagcg ttagataaac aagagaagaa gatagaaagc ttcgctaact
     6361 tcgatgcaag tgaaaggtgg taagtgcatg atcatcaatg acaatggcag agagtatgat
     6421 acagaaaaga ttgaagagta ttcatcttat actcagggat taattaaacg tttgatatac
     6481 gttcgctatg taggtattag ggatctgtta tcagataact gttgtagtaa atacaaagtg
     6541 aatcaagtaa gagaagcgtt gaataaagat aataacgtcg aaagaataaa aaatgttttt
     6601 ggatatagta ttgaagagat taattattac attgacttcg ctgaagcttt cattccgatg
     6661 gtgagataac ccccccttaa aataaatcgc aaattttttg ggggtgatga aacggagggg
     6721 gctgtcagga aaagagattt tttcgaactt tatcatgaaa ggagggctaa aatgtttaaa
     6781 aacgaattgt ctcaaaatcg ctacagagaa aaattacgcc gctctttaat aagccaattg
     6841 gaaagtcaga aaacaaatat tgagccattc ttagataatg ttgatcgtta tatcagttta
     6901 tgggaaacgg cgatatcact ggaagaagat atatccgaga acggcattag actggagaat
     6961 ggtaaaaaga atgaatcagt agcgttgctt gtttctgtca acaaacaaat gggattgatg
     7021 ttggataaac ttgccattac tcctgaattg gtaggtgaag caaatgaatc aattcctgag
     7081 ttataagcat attgaaaatt ggttcaaagc tatagaagaa ggcactatca aggtatgcaa
     7141 agagcaatta ttgctaaaaa attatctaga agaaagagtc tttactagag aagatattta
     7201 cttcgataag cagatggtag aggattcaat caatatacca gcacaatact ttccattcga
     7261 attaattccg tgggaaaaat ttctacaatg ttttatttat ggtgttcgat ggaaaaaaga
     7321 taaaacacta gtgttcaata gatatctttc attaatggga cgtggtaatg gtaaaactgg
     7381 ttttgcttct tggaacaact tctttctact aaccgctaaa cacggtatta aaaattatga
     7441 tattgatatc tatgccaata atgaaagcca agcaaagact agttttgatg atgtatttaa
     7501 agtaattaaa gatcatcctg atttagataa aaaagtattt aaagctacga aggaagttat
     7561 tcaaaatatc gctacaaaca gcaaacttcg ttataacacg gcaaatgcta gaacaaaaga
     7621 tgggaagcga ccaggtgcaa accgctttga tgaaattcac gaaaatgaag attattcaat
     7681 gataaatgtg gctacttctg gtggtggtaa aattcgagat tatagagaat tttatgatac
     7741 aactaatggt catgttcgtg gtggtccgct tgatgacatt atagaagaat caaaaatgat
     7801 tctttctgga gaacttggaa ttgacaagga tggagcagaa ttttctagtt tgtttccatt
     7861 tatttgtcgc ttggataacg ataatgaagt tgatgatccc gacatgtggg aaaaagcttg
     7921 tccaactatt aattacaatg cagatttaaa acggaaaatg tttcaagaat actctcaaat
     7981 gcaacgtaat gctggtttaa gacttacgtt catgaccaaa cgaatgaaca gacctatgga
     8041 agatacacga tttgctgttg cttcatatga tgatgttctg catacgaaag aaaaagaatt
     8101 tcctgaaaaa atggatgaag tgataggaac agtcgatttt gctgatagac gagattttgc
     8161 cagcgttggg ttgctaggaa aatacgataa agatgtttat tttacacaac atacttttat
     8221 ccacgaatca gcccttcgat tacaaaacat caaacgagag gttatagata tttctataga
     8281 tcaaggaaaa tcacagatcg ttcatggaaa aaatatagaa gctgattata ttgtaggttg
     8341 gtttcttgaa atgagtaata aatattatat taaaaaaatc gctatggata tgtaccgtgc
     8401 aaaaatattg aagcccgctt tagaagaagc aggttttact gtggaaattg ttcgaagcgg
     8461 atctgttaca catggtatgt taaaagatct ggttgatgac ctttttatta atcaacgttt
     8521 attttttggt gacgatgcga ttatgcgttg gtattgcatg aatgtatatg aagagcatat
     8581 ttctaatgga aatatacgct atgaaaaaat agaacctgaa actagaaaaa cggatggctt
     8641 tttttcattc cttcatggtt tgaatttttt agatgatatt tatgattctg ctcctgtaac
     8701 agtcacaaat agctcagtag aaaatacagg aactggattt actcctctag tattctaact
     8761 tgaaaggagg tgagaaagtg gggatttttc aaaaggcggt aggatacttc acaaaaaaag
     8821 caacggttcc tttagaagaa tacttttgta aattgcaagt tgattttgtg tatcgaaaat
     8881 ttgcgattga aacttgtatt gatttgattg caaatgcgat gagtaaagcg gaattcaagt
     8941 catatgaaga tggaaaaaat aaaaagaatg atctttacta taggctgaat gtagctccta
     9001 ataagaaaaa taatgcaaca gaatttagaa aaaaactgat caggagatta atattctaca
     9061 atgaagtatt gatcgtttct ccgtctaata attctagcga aatatttatt gcggatagtt
     9121 gggatgtcac agaatatgca ttgaaagatg atgtgttttc tcaagtgcaa attaacaaca
     9181 tagtccttga tagagaattt ctagaaagtg atgttatcta tataaaatac gcagatcaac
     9241 aaattaggca actagtcgat gcgtattatc aagcgtatgg gaaactcatt tctagtgcca
     9301 tgaatgttta caagcgctct aacgctcgta gatacgtact gaaagggaat ttattccgac
     9361 cgcaagacaa tacaacacaa gatcaaatca ataaaatgat gacatcacaa tttaaggctt
     9421 ttatggaagc tgataatgca ggtgcggtat ttcaattaca aaatgagtac acattagaag
     9481 atttcagcgg aaacttccaa agcaattcaa gagatataaa aaacttaata gacgacatct
     9541 ttgagatgac agcagcagcg tttcacgttc cgaaaaacct actaaaggga gacatgagtg
     9601 ggttatcgga tcaagtggac gcttttttaa tgttcgaaat cataccgatt gctgaactta
     9661 ttcaggatgc gtttaacgct agtctctatg aagtagaaga atacttgtca gggaattttg
     9721 tacgtgtgga tacaactatg atcaagatta ctagcttcaa agatttggtt gacgctattg
     9781 atgtaggcat tagaaatgga gtatttacaa tcaacgaagg aagagagcgc gttggaaatg
     9841 atcgctctga taaggcgatg gcagatgaaa tatttataac taaaaacaat caacaagtat
     9901 cgaaaggagg tgaggcgaat gacgacaatg aaaacatttc tagcagtaaa gaatgaaggc
     9961 gcagtaccgc aaatttttat tcagtgattt attggttcta gttggttctt tgaagggaat
    10021 actgacaagg gaatcaaaaa tattttggat agtctaggtg atcaagaaga aattgaagta
    10081 gtaattaatt caaacggtgg agacgtattt caagggattg ctattgggaa cttacttaag
    10141 tcaaataaag caaaagttaa cgttgtgatt aacggcttag ccgctagtgc tgcttcaatt
    10201 atcgcaatgg ctggcgatac tataaaaatc tacaacaatg cacaattgat gattcaccgc
    10261 gcttccacat acggagaagg aaatgtcgat gacttccgta cgattgctga ccaactggaa
    10321 tcaattgata aatcggtaaa ggcttcatat aaaacacgat tcaatggcac agatgaagca
    10381 ttgcaagaac ttcttgaaaa agaatcgttt atggatgcag aaacagcttt gagttatgga
    10441 ttggtcgatg aaattatcga tgcagaaaat agctcaggta ctgaagctaa aaaagaacaa
    10501 agcgttgaag aaattttgaa tgacgttaaa gaaaaaagag cagaaaaaat tgctgcattt
    10561 acagcagcat taaataaaac atttggacaa ggagatgtaa aataatgaca gttaaaaatt
    10621 taaaaggtgt aacagctgca agcgaccaat tgatgaaagc ttttaaagat ggtaacgaag
    10681 aatcttttag cgcagctatg gtaagcttat ctaaggaaat tcaggataaa attttagaag
    10741 aagcaacagc aaaaaatcaa gatcaattag tattaatgaa ccgtggtcag cgtgtattaa
    10801 ctacacaaga aacaaaattc tataacgaag tagtgaaaaa cgaaggtttt gcaggggtcg
    10861 aagaattagt gccagctact gtatttgaac gtgtatttga agatttagaa caatctcatc
    10921 cactattgca aaaaattact tttgttaaca caactggtgt aacagaatgg attgtgtcac
    10981 gtggagtcaa tccagcatgg tggggtaaac tttgcgaagc tgttaaaaaa gttttagata
    11041 atggctttga tgtaattaac atgaagcagt tcaagctatc aggttatatt cctgtatgta
    11101 aggcaatgct tgatttaggt ccagtatggt tagatcgtta tgtccgtact gttttagtag
    11161 aatcattgag aattgcatta gaacaagcaa ttgttgatgg tactggtaaa gatatgccag
    11221 tcggaatgat gcgtgacatg agcaaacaaa ctagcggaga atatgctgaa aaaaaagcag
    11281 aacctattac agctttagat gctgtaacta tgggcggttt gatggcgcga ctatcaaaat
    11341 tcaatatcga aggtgtgaat gatccgattt atcgtaatgt gaatccttct gatgtggtcc
    11401 taattgtgaa tccaactgat tactggtcta aagtattccc agctaagact gtactaactg
    11461 ctaatggaga atacgtacaa gtattgccag taccagtttc agatttgcag tcaacggctg
    11521 tgccagaagg aaaagcagtt attggggtag cctcagatta cttcatgggt gtaggatcta
    11581 cactaaaaat tgaagcttca gatgaatacc attttgttga agacgaacgc atttatctag
    11641 ctaaacaata tgcaaacggt caacctaaac gtaacgatag tttcattgta ttagatatta
    11701 gcgctttggg aactactact acaactacaa aaccaacaac cacaacaact acaacacaag
    11761 cgtaggtgat cagaatgaag tatattcttt gtcagccggc aatcaatcgg tttaaatggg
    11821 agcttgaagt ttgtttaact aatctgaaga aactaggaat caaagatatc gtattgcttt
    11881 tcagcagaca cgatgatcag attcctattt tttttgagaa ggaatatggt gttgaagttc
    11941 atgtgtacga tgatctgcgg gacgacaaag agtatattcc ttcgattaaa ccatatttat
    12001 ggtggaaata tttagaagaa gatcattcgc gtgaggacga ccgatatttc tatatcgatt
    12061 cggatgtcat tttcaataaa agaattaatt tgcgcaaatt gccttctaaa gatgatgttt
    12121 ggtattgtag cgactgctgt agttatctaa gtcttgatta tattagaagc tgtgaaaacg
    12181 gagaaaatat tctaaaagat atggcaaaca ttgtaaatgt tacagtagaa tctttggaaa
    12241 ctataaacac taattcagga ggcgcatagt gggttattaa ccgccctaaa gcgaattatt
    12301 ggaaaaaggt ttatctggat tctaatcggc tatatcgcta ccttagaggg caaaaaacaa
    12361 atatacaaat ctggacagcc gagatgtggg cacagctttg gaacatgatg tatttcaata
    12421 ttggtcctaa agttcacgag gaattagact tttgttttgc tactgatcca atagaaaaag
    12481 ttaaagaagt aaaaatctta cacaatgctg gagtaacaac aaacgatgaa gatttatttt
    12541 tcaaagggag atacgtgact tctacgcctt ttgatgaaga tttatcattt gtaaacaaga
    12601 aaaaatgctc ttacgcatat gttaaagcaa ttaaggcggt ggttagatga cgcctgaaca
    12661 agtgactgaa gaattgctaa tagctgtgaa ggataatatt tacgttacct ggaacgaaga
    12721 agatgagtca attaaaaaga tgatagctaa aaatgctgtt tatcttcaaa gtaaagtgag
    12781 tacaacactt tctttttctc ctgaaagctt agaatacgga ttgctaatcg aaagatgtag
    12841 atacgactgg aatcgtgctt tagatgagtt tgaacaaaat ttcgctagtg agttattagg
    12901 tttcattcaa cattatgcgc tacaagaata tattgcaggt gatgtgaatg gcgaataatc
    12961 gtagactcga agaaacattc aacgatggtt ggttaaagat tttgacgcaa actaccaaaa
    13021 gaaatgaact aggaaaaaag attggtgtag aagatacaga aatcacttct ttaaaattta
    13081 gaaatctttc catgagagat agtgatataa cagctatgga tgcgatggga tcgaaattaa
    13141 ctaagaaagt aaagactcca tttcatccaa tcgccaagaa atttaataaa gatcaatatt
    13201 ttatcgtaat cgatagtatg cgttacaacg ttatctatgc cgattacgat aatttttata
    13261 tctattttta tcttgaaagt gtgggtgaat atggtgattg ataattctaa agaaaaagaa
    13321 cgtttaaata agcaaatttc tgctatcaaa acttccttag aagagcattt tggcctcaaa
    13381 ctctttcaag actccgtagg cgaggatgag ctacctgatg attttaatta cttcattctc
    13441 gaaacaggag aaatagaaat gatcactgag ccaaaatata gcgtgggtca aaatctatat
    13501 ctaactttct attcagaaaa tagagaagat ttaacaggag attcactaga tattatttca
    13561 ttgattcaaa atcgttcgat tcgttttcag agaatggatc ccaatcattt aaaactagag
    13621 aaccaagatc gctatatcga tcaattggta tttacgttta gacgattatt gaagagtgat
    13681 tgtcatggct aaaaatagtt gggagctaaa aataaatgga catgatgaac ttcttgtgcg
    13741 gatggaacgc tattcaagcg agagcgaacg actgattaac gaagcattga aatcaaaagg
    13801 ttcggctatt gcagtggata ggattacaga aaaaattcct gtttctgaag cagatttaag
    13861 aagagggcac caacacgcaa aaaatagtcg tccacttaag actcaataca ttaatttggg
    13921 tttcatcatt agacctacaa gaaaatttga gtatttaaaa tatcctgatt tggggatagg
    13981 tacttctaaa agaaatcagc cggacgaatt tatgagaaga ggattaggtc ttgcacttga
    14041 tccaattaca gaacttctga ttcgtcaatt cgataaatta aataaatagg aggaacaaca
    14101 atggctaaaa caacaacagt aacaactttt gacaatataa gtattaaaag aatttctttt
    14161 aattttaaga atgcaacaaa tgcaatttca actgattgta atggacaatt agatggcgaa
    14221 acagaaatgc agagaattgt aaaaaaatgc ggttcaacag aagtaaaatc gaaatctaaa
    14281 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgcttc
    14341 aatgggttga aacaagatga acgtattaaa tcgggaattt actcttatgg tcctgattcc
    14401 gtaggggaag atttctctct tgctgcagag atcgtggatg atttcgaaga aaaaaataag
    14461 ctgattggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14521 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14581 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14641 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14701 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14761 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14821 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14881 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14941 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    15001 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    15061 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    15121 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    15181 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    15241 aactatgcaa taaaccaatc ccagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    15301 gagaacagaa gcagaagtaa cattcatagc taacgatgac ggattgaaat ctacgttaaa
    15361 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15421 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15481 gtatgaatta caaagtcaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15541 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15601 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatgcac tagctcaagc
    15661 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15721 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag caactgctgg
    15781 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15841 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15901 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15961 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    16021 cggaagcaaa atagatatga ataatctaat ggaagcttct gacgttttaa gcgacattgg
    16081 cgataagctt acagaattag ggaaacaagc agtggactct gctaatagtg taggtagttc
    16141 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    16201 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    16261 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatatcac
    16321 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    16381 tgtaaatggc ttaatggtca atttcggctt gagcgctcaa gatgcaatgg atttaatggt
    16441 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16501 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16561 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16621 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16681 aaaagctttt gaagagtgga aaaatggcgg aggaacacaa aaagacgtta ttaattccat
    16741 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16801 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16861 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16921 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16981 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    17041 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    17101 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    17161 aataatagga gcagttatta ctgttattgc tggagttata gcaattataa aaaactgggg
    17221 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    17281 ttgggaaagt ataaaagaag gtgcctcatc agtttgggat ggagttaaag aaacctggtc
    17341 tggatttgta gattgggttc aagatatttg gcaaggagtt tctgattggt ttggagagct
    17401 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17461 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17521 ttgggcagac attgtaggaa ttgttcaaat accatggacc ttaataacgt cattgattca
    17581 agccggtatt aatattatcg tgggtatttt tgatgtagct ggacagttat taggcgcagc
    17641 ttggcaagct gtttggacac ctatttctga tttccttaaa aacacttggg atactatgac
    17701 acaatgggta agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17761 agtagtggca tggtggaatg gtatatggac agctattagt actacggctt caaatatttg
    17821 gaattcaatt agtgcaacag cttctagtat ttggaacagt atcaagaata caatcactag
    17881 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17941 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggaata gcgtgactag
    18001 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    18061 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    18121 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    18181 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gtacaattgc
    18241 taacatttgg gaaggtatca aaaagactgt tagcgctcca attgattgga tcagagataa
    18301 aatcagtagt atctttgata atttgaatat ttctatacca catattccgt taccacattt
    18361 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacgttgg gtgttgattg
    18421 gtatgcgaaa ggtagtgtgt ttaattctcc gaatattatc ggtgtcggcg aagcaggacc
    18481 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18541 tagcacatca gtttcatcta gggcacaaac gattcaacct gtgaataact acgaattcaa
    18601 tttcacaatt gatggtaacg cagatgaggt tactatgaag caaacaactc aacaaatcat
    18661 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18721 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18781 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18841 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagta
    18901 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18961 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaattatgca
    19021 aagaaattag aattctctga tgatcccaac gtctattatg ttatccagac tatcgatatt
    19081 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    19141 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    19201 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    19261 tcatgccagt tcatcttaaa tgattatgtg tttagttttg aaggcttagt agtaggaact
    19321 gacgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    19381 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatattaat
    19441 tattttaatt gtactggttt gagcaaaata caaatttatc gtaatgggtt aaggtaggtg
    19501 aaatagatga tcgataattt aataactatt tacgataaaa acgacgcgaa taatttagct
    19561 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19621 agcaataaat taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19681 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19741 cagcgtctac ggatttatta tgcaaagact tctgtaatag gcaatacgat agaagtaaaa
    19801 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagtggaaca
    19861 gaaaagatca ctgctagtca ggcatggcaa aacgcaaaaa ctttagcgaa accagttatt
    19921 ccttcacagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaag
    19981 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    20041 gaatttctaa aagataataa cacattacgt catgaaaaaa ggctaggcac ggatcataaa
    20101 atcaaagcga tttatactaa aaacttaact ggtcttgact tagagattga tgctcaaagt
    20161 gttttagttg gagtttatcc attcattagc agctcttcag aaggagaaga cgagatcact
    20221 ctaccagaag aagttatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    20281 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    20341 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    20401 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20461 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20521 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20581 aacttcttag aaaacacaga gaataatgtc agcaatttga ttaatgatgc cattgatcaa
    20641 ttgaaaaacg gtggcgaaat cagtgattta ctcaatgata ttgtagatca tcaaactgat
    20701 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20761 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20821 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaacggc atggacgtta
    20881 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20941 ggtactacat taattagtga tggtgctgat tatagaacaa gtattgctaa tggcaaaatg
    21001 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    21061 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    21121 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    21181 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    21241 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    21301 actatgaaat ttactagtga tggtatgcta acgttaccag gttgtttttt tggttcatgg
    21361 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21421 gttagaggta ctaaaaactc aactgtacca acagaacatt atggacaacg actattgaac
    21481 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgacgat
    21541 ggtaaagttc gtgttgatat tgaccccatg tttgctgaaa cagtaaatct aagtcggtat
    21601 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21661 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21721 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21781 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21841 atttggcttt agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21901 gtgataaagg gattacggtg acggttaaac cgtttctaaa tggcttgcag atggatacga
    21961 gtggcggaac atttacttta aaaggaacaa caccatctaa ccgttacgta gataatgttg
    22021 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    22081 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    22141 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    22201 atgtttcgca attagaagag ttgattcgaa agtacaacga aacttttgat gcttttatgg
    22261 ctgaaatcaa aggtagagtg gatagcttaa atcaacagat tactgattta actggtcaag
    22321 ctaaaacgct acaagacaag ttagatgctc tgaaagaaga aatttctaag ttaggtaact
    22381 tacaagtgat gtacagtaac agcatcgact tcgggggata tgattatagc ggtaagccga
    22441 atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg tcactaactt
    22501 tggataacga aaagttacat tttacttctg atggtacagg aagcattatt atgtttacgc
    22561 gtattaatac acctcagctt actagtggga aaacctatac tctgagtgcg aaagttcgat
    22621 ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt acatcaccag
    22681 gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat gtagggaaag
    22741 aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta gatcgatttt
    22801 atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag ttgtacgaca
    22861 tcaaaatcga agaaggctca acagccacac cgtaccagcc taatctactt gatgcaccgt
    22921 attatttgag taaggtggct ttgggtgaga atctaattaa accagaatca caacaaccag
    22981 ttactaatag taactatctt attaacacct ataacattaa accaatggta aaaggtaaga
    23041 agtataccat cacacttgaa ggaactaagc cagcaacaca ggtttttaga ccatttttca
    23101 cacgagcaac aggagatgca tgggaggttg gtgacttaca accagtagaa ggcttaacta
    23161 atgtgtggtc taaaacattt acagcagcag atgactcaca ccctactacc ccacaagtgc
    23221 agatttatca agtaccaagc acaagtgtag gacaatgtac aattaagtgg ttaaaactag
    23281 aggaaggcaa tactcgaacc ccgaatatta gtgagtataa atatcgtggt actggtatgc
    23341 gtgattcaaa caatccaaaa gattatgttt gggatctagc accagaatat gtcgaagata
    23401 atttggccac agatattaaa atttctgaaa ttactggtaa agcaaacaat tataccgatg
    23461 ggaaagtatc ggagattaat tcgcagttga ctgcttcaat taatgaagta gacaccacag
    23521 ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa ttagacaata
    23581 agatcgatga acgcattaat gatacagcta ctaccacatt aacagttaca aacgggaata
    23641 ccggatcagc aaagctttat cgtgaaggaa aaacagtttc tatatatttt gtggctttaa
    23701 acggaaaacg cagtggtgga aatgattcaa cgatactaac aattccagaa ggctatcggc
    23761 caccaattag ttttgagcaa ctggttggct cgatagaccg ttctactttg aacagtgctc
    23821 agttatctat tggtgcagat ggagccatta aatggcgaag aaactcaagt tatggatcgg
    23881 attatacctt tgcaattact tacacgattt agaaaagcgt gaatcgatat gaaggcagca
    23941 tatagaccaa ttgaacctta cggattcgag caaatcattg tgaatgatga agaacattta
    24001 ccggaagaat gcacagaagt cgaaccaccg attccaaatt ggaaaccgaa attcaattac
    24061 tgggagggaa ataaatgaaa aacatttgga aatatggacg tactggcgga gagtacgcag
    24121 gaaaagtatt ggacgacatg cttgtatccg ttccttacac agatcagcca ccgcttgaag
    24181 gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca aatgtttgat cctaaattga
    24241 atcaatggat tattttagcg aacgcactag atcacaacga tttaaacaat ctcaaagcaa
    24301 tgtatgagtc gttagaaaat gagaacggcg atttaaaaca gatcaatgcc aaactcatgc
    24361 taagcgatgt agcaattaaa caggaaaata ctgcattgaa agaaaaagcg gatagtttag
    24421 cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa tagcaaagac atttcagaaa
    24481 ttaaagagca actaaatcca gcttcaaagg gaggtgagta gtatgtttag ttttagcgat
    24541 gtgaaaatga tgtatgattg gggctgtttc actaacgaac aagtaatggt tttcgttccg
    24601 ttgtgcatta ctgaagaaaa agcagataaa atcattagca aagaagagag cgcatcttaa
    24661 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
    24721 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
    24781 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
    24841 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
    24901 gaaacacgat gaaagattag atacacatgc agaaaaattg gtagaacaca cgcaacaaat
    24961 taaaacttt
//
LOCUS       DACEPW010000023        24371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEPW010000023 DACEPW010000000
VERSION     DACEPW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.023"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IW819_000728"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IW819_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1243853.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="IW819_000729"
     CDS             complement(1551..2972)
                     /locus_tag="IW819_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1243854.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IW819_000730"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IW819_000730"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1243855.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FTDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="IW819_000731"
     CDS             complement(5383..6639)
                     /locus_tag="IW819_000731"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1243856.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7219..8520
                     /locus_tag="IW819_000732"
     CDS             7219..8520
                     /locus_tag="IW819_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1243857.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8589..9188)
                     /locus_tag="IW819_000733"
     CDS             complement(8589..9188)
                     /locus_tag="IW819_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1243858.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9442..10323
                     /locus_tag="IW819_000734"
     CDS             9442..10323
                     /locus_tag="IW819_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1243859.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10336..11946
                     /locus_tag="IW819_000735"
     CDS             10336..11946
                     /locus_tag="IW819_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ1243860.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12023..12682)
                     /locus_tag="IW819_000736"
     CDS             complement(12023..12682)
                     /locus_tag="IW819_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1243861.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12965..13630)
                     /locus_tag="IW819_000737"
     CDS             complement(12965..13630)
                     /locus_tag="IW819_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243862.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13777..14382)
                     /gene="dhaL"
                     /locus_tag="IW819_000738"
     CDS             complement(13777..14382)
                     /gene="dhaL"
                     /locus_tag="IW819_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1243863.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14410..15393)
                     /gene="dhaK"
                     /locus_tag="IW819_000739"
     CDS             complement(14410..15393)
                     /gene="dhaK"
                     /locus_tag="IW819_000739"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1243864.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15395..15790)
                     /gene="dhaM"
                     /locus_tag="IW819_000740"
     CDS             complement(15395..15790)
                     /gene="dhaM"
                     /locus_tag="IW819_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1243865.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15944..16264
                     /locus_tag="IW819_000741"
     CDS             15944..16264
                     /locus_tag="IW819_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1243866.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16376..17245)
                     /locus_tag="IW819_000742"
     CDS             complement(16376..17245)
                     /locus_tag="IW819_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1243867.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17341..18615)
                     /locus_tag="IW819_000743"
     CDS             complement(17341..18615)
                     /locus_tag="IW819_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1243868.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18641..19150)
                     /locus_tag="IW819_000744"
     CDS             complement(18641..19150)
                     /locus_tag="IW819_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243869.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19325..21460)
                     /locus_tag="IW819_000745"
     CDS             complement(19325..21460)
                     /locus_tag="IW819_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1243870.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21543..23198)
                     /locus_tag="IW819_000746"
     CDS             complement(21543..23198)
                     /locus_tag="IW819_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1243871.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAETLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23200..23514)
                     /locus_tag="IW819_000747"
     CDS             complement(23200..23514)
                     /locus_tag="IW819_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1243872.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23684..23944
                     /locus_tag="IW819_000748"
     CDS             23684..23944
                     /locus_tag="IW819_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1243873.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23958..24272
                     /locus_tag="IW819_000749"
     CDS             23958..24272
                     /locus_tag="IW819_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1243874.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6945 a         5038 c         4393 g         7995 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atctttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 attccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtt gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacga tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtctat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg tgaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacgtagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctact tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcagaattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcatg tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttaaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaacc aaaaaagccg aactgacatt ttatcgtcag tttcggcttt
     8581 tttatgcttt atttcttctt cggtgcgatt cccttgatca ggacttctgt ccattctgct
     8641 gtatatggaa gcagttttgc gtatatctcc ggcttagaaa ttccttctac cattgcttga
     8701 aaatagagga tcaagaactc atccgaatat ttcaaatcca cttttccttc tttccgccct
     8761 cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg tatacgcatc catcgcgtca
     8821 gaaaattctt ttttttcttt ttttgtatag tatgtcatca aatcgatata aaattgtttg
     8881 ttcactcgat tgagataacg gattttgttt tcactcatgg caatcagtgt ttcttcaaat
     8941 ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta aatcattcat aaatgcaaaa
     9001 aatacttcat aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt cgctttactt
     9061 acctgagcgt tctcagctac cttatccatt gtcactccag cggttcctat gtctgtgttc
     9121 atcaagtcaa aagctgcttg taaaatctct ttctttttct cttgtgctct cttttcaaaa
     9181 ccgttcattt gtagagcctc ccgtaatttt aaacttttat aatatatata gttcaaattt
     9241 attctgaggt ttattataac tatctattga gaaagtatca agctagcaat tccttgttta
     9301 tgaattcatt gacagaaatt tataaaaaga atattatgta ctaaaataat aaaaaaagtt
     9361 ttataaaaag gtataaaacc tttttattaa actatttaat cttgtttagt atatattttt
     9421 agaaaaagga gtggggtttt tatggcagaa atcgtcaaag tcacacattt gaaaaagagc
     9481 tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt
     9541 ggatttatcg gaccaaatgg ttctggaaaa tccacgacga ttcggacact tttagggatt
     9601 ataaaaaaag acggcggaac ggcaacgatt tttgggcaag atgtatggga agatagttta
     9661 tctatccatg aacgtctttc ttatgttcca ggcgatatct ctttgtgggg aaatttgact
     9721 ggaggagcga tcatcgatct cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa
     9781 gatgaactga ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa
     9841 ggaaatcgcc aaaaagtggg cttgattgct gctttggctg ttgattcgga tctctattta
     9901 tttgatgagc ctacttctgg tttagatcct ttgatggaaa cgattttcca agaagaagtt
     9961 gaaaaattga aagaacaggg caaatccatc ctactttctt ctcatattct cagtgaagtc
    10021 gagcgactag cggataaagt cgtgatcata cgcgaaggaa cagtcgtaga agaaggaaca
    10081 ctcgatgaat tgcgtcattt gactcgttcg accatcacta tagaaacaga agcagagaat
    10141 agtcacttag cacaattgaa cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa
    10201 tttgctgctg ataacgaagc tttcgatgtg attttagctg aagcgtccaa attaggtgtg
    10261 aaaaaatttg aatctattcc accgactctt gaggatttat tcatgagaca ttatgaagga
    10321 tgaggaggaa gcgaaatgac tgataaattt tcacgctggt ccaccctttt ttctcaaaac
    10381 cttagccgtg actggaaaaa gatcctgata tggctagtcg gtgtcactgc tttttctggt
    10441 ggttttgtcc ctgcgtttga agaattgact tccggacaag gattagctgg attatacgaa
    10501 accttgcaaa acccggcaat gattgcaatg gtcggaccaa ctccagcttc gaatgctgct
    10561 gagtatactt taggagcgat gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct
    10621 gctgtgatct ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga acttggtctg
    10681 attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct
    10741 gaaagtattc tgatcaattt gcttttagca atatcgatca gctgtacgat gatttttttc
    10801 caagcagatt cgatcactgc agaaggtgct tggctttttg ggctatctgt aggtagtatg
    10861 gggattttgg ggagtgccgt tgcactgttt gctgcacaac tgatgccaag ttcagccagt
    10921 tcgaatggtc tgtctttagc gtttttaggt ggactttacc ttctacgagc aataacagat
    10981 gtagctgatt cctccctttc gatgtggaat cctttaggct ggtcttattt gacctatcca
    11041 tttacaacta acaactggat tcctttgctt tatttgtttt tgttcagtct aatcttgata
    11101 atcagttcat ttgttttaga ggaacatcga gatttgaaca ccggctatat ccccgaacgt
    11161 gaaggaaaac cgtatgctaa acgttctctt ttatccttgc gtggtttatt attctatcta
    11221 aataaaggga tgacaatcag ttggcttatc acttttggtt tgatgggggc ggcatacggc
    11281 tctatctacg ggaatatgca acagttttta gaaagtaacg acttaatgaa acagatgttt
    11341 atccaatctg gcgtctcaat tgaaaaaaac tttacaagca cgatcatggt cgtcttgatt
    11401 tgtctagctg ctattttacc aattgctagc atcaatcgtt tatttacaga agaaacccac
    11461 ggacgtatga atcagctgtt cgcaacaaaa atttctagga accaacttta ttggaccaat
    11521 atgttactag ctattctatt tgctatattg gcaatcctga ttagttctgg tgcactaggg
    11581 ctttctgctt tggcttcgat gaaggatgcg aaagacatgg atctattgat gtttcttaaa
    11641 gccgggttca atttcctgcc tgccgtttta gtgtttatcg gaattagtgg ctttctattc
    11701 ggatggcttc ctcgaatggg taaacttgct tatatctact tagcttactc ctttgcactc
    11761 aattactttg gcggaatttt ggatttacca gaatggtttt ccaaaacagc tgtccctagc
    11821 tgggttccac ggatgccaaa cgattctttt gatctttccg tgttccttac gttatcatgt
    11881 atcagccttg cactgttcgt ccttggttct attggttata ggcgaagaga cttactagaa
    11941 ggataataaa aaagcatgta caatttcatg ctagcctcca aatctgggtc aaaaatccaa
    12001 catttggagg tcattttttt atttacaagt cttctaactg ctctttatca atgatccaga
    12061 cttctcgatt ctttcttttt acataaccat tctcctctaa taatttaaat tttctagata
    12121 aagtttcggg ggtcgtaccg ataaatgcag caagttcttt cattttcatt ggcaggaaaa
    12181 agtgatcctt ccctgtcgct ttagctaagt caagcagata gacagcgact cgttcttcta
    12241 ctttttccat catcaaaaaa gttgtctgct gctgtacttt tgttaatttc tcaacgttga
    12301 ttgctaaaag tttcagacta agggcgggat aatgcagtaa gagttccgag aaatcttttt
    12361 gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc aaataaagac tcatttggaa
    12421 cgcctagcaa ctggctctct ccctcatatc cgcccggctc tattactcgt aaaagctgtt
    12481 cctttccgct agcagataat tgatacactt tcatactgcc ggaggccacg atcactaatt
    12541 gaggttctcc attcggcgca agaatctgtt ctcctttttc cacatagtga tgtttgacaa
    12601 gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa
    12661 cacataaatg atcatgactc ataatctctc ctccttctaa acagctattt ctaataaaga
    12721 aacataactt cccttattcc aactgataat tttcttttat catagcataa acgtcaagcg
    12781 aaccagatac cagcaaatag agtggatttc tttttgtccc ttgctttatt ttccttcttt
    12841 tgagacctta agtaattgat tcctatcaat tttttaaata tttacttttt gaataatcaa
    12901 aaacaaacct tcactcgcaa gccgagagta aggtttgttt ttcgcattgc ttatttcgtg
    12961 ttgtctattg aaatttttcc gcacctagct tttgatatag tggtgctagt tcttgtaagg
    13021 tttcagcggt tatttgctca acgtgttcga tttcttgcgg atagaagaat agtttaccca
    13081 tttgaaaggt tcggtctctc atcgcttttt tcaactcttc ttgtttcttt gtatcggaca
    13141 aatagtcaga caaagctaag tggtcgtcaa gttcatgttc tggttgcggc catatgtaat
    13201 aatccttgat atcgactgtt ttgctccact ctttcagtat cgcaagcaac tggttatacg
    13261 ctgcaatgct accatatcgt tcttcgcttt tataatgttg gtacatcaaa tagatttggt
    13321 attgtttttg attcaacaaa actgctagac aggcattctc gtcttgacga gattcgcttc
    13381 ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt cgtccagctt tcgattttag
    13441 gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt ctggagctga atctctttcc
    13501 atttttgcca gtgttcttta tattctttct tgatttgttc aatcttatct gttggatggc
    13561 tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa caactgataa tctgattttc
    13621 caagttccat tgtttcgcct ctttccgtta tcttttgtat tctacctgaa ttttgcgata
    13681 tggacaaata gaggttgtga aaaagctgtt ctgcatcaag tcataagaac agccaaacaa
    13741 aaaactgcga tgagcatcac cgcagtttta ttgaacttaa ggttgattga tcacatctaa
    13801 aagcgttgaa aataaaatgg cactagaagc agctcctggg tcgatatgtc cgattgcacg
    13861 ctctcctaga taggaagctc gtcctttttt agctaccaag actttggtgt gttcttttgc
    13921 ctggtcaatg atttcttttg ttaaattccc tgccttcaat gcctctgtga ctggcagcca
    13981 aacatctaac atcgttttat cgtcagcttc tgcctttcct cggctttgaa tacccgctgt
    14041 tccctgctcg atcacttcgc ctaatttttc ttgagaatca atagaatcga cctcttttgt
    14101 cgctttcatc atgttcatga atgcagatcc gtataacgga ccagaagccc caccgacttt
    14161 cgatatcatc gccatagaga atactttgaa tgtatcgctg attgtttcag gatgatcggc
    14221 ttgaaaagct tcttcataag ctgaaacccc tcttcccatg ttatttccat ggtctccatc
    14281 gccaatcggg gtatctagat cactgagata ttgtttgttc tgattgattg cctctgcaaa
    14341 ttgcgtgagc cattcttgga tttcttgaac ggttagtttc atattatcac ccttttatct
    14401 tgagattttt taccatgaaa tcgtctgtac attgctttct aaagaatctt tccattgtgt
    14461 ctccgtcaag tcgatcagtg ttagagaaag accttgcata tctagtgagg tcataaaatt
    14521 accgactttt cggaaaacta cattgacgcc tctttcttcc aacagagcta aaacatcatt
    14581 catgaacacg aactgttcca tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc
    14641 ttcttttggt aaattcgagt aatcatctaa aatcttagta acaagttcct ttgccagtac
    14701 tttagaaggc tgcatctttt ctcgacgata gcctggctca ccgtggatac caacaccata
    14761 ctcgatctca tcttctccta gttcgaatcc tggtttccct acttctggta cagttgctgc
    14821 tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc ttttctccca gttctttcaa
    14881 ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg attttgtgta caaggacagt
    14941 acctgcaact ccgcgttttc cagcagtata cgtactattt tcaacggcga tatcatcatc
    15001 tacgatcaca ctttctactt caatatcgtc catagcagct aattcctttg ccatatcgaa
    15061 gttcaatgca tcccctgtat aattcttaac gatcagcaaa actccttttc cactatccgc
    15121 ttccttaata gccgtctgaa tctgatctgg agttggagaa gtaaacacat caccaagcac
    15181 cgctgcactt aacatccctt cccctacaaa cccggcatgg gatggttcat gtccgcttcc
    15241 tccaccagaa accaaaccaa cttgttccgc tttctgattc ttggctacaa cacgggaaga
    15301 ttctacacga tgaacaagtt ctggatagct ttttactaat ccttccagca tttcttcgac
    15361 aatattgctt ggatcattca tgattttttt caatttattc ccatcctttc tttccggcat
    15421 tttgtgcttc tgttaagatt tgctgagtat catctgttat accggcagtg atggctgctg
    15481 caaaagctcc ttccactaaa ggcgcatcca ccagatgata acgttgctgc tgctcttctt
    15541 ccaacatgtc ttttgccagt tccgagttca ataccgcgct accgatatcc gcaaaaacaa
    15601 aataactagc tgcatcgctt gattcattga ctgcttctac gattttcatc gggtctgatc
    15661 cgatctcgcc atttattccg ccgagagaaa aaatttgcac ctcttcggat ttttgcatct
    15721 gctcaatcat ttctttaata ccatctgtta ctttttgact atgagaaata agcaaaatac
    15781 tttttttcac aagaatcttc ctttctttta catagttgct aacgattggt ttcgctttca
    15841 ttaatgaaca attcaagtat ataataaatt tctaattttg ggaaaatatg tgtagtgctt
    15901 cttgaagttt gttatacttt aattaaaaga gaggagtgat cgaatgatta caacaacaac
    15961 gaacagtgta gagggaaaag ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat
    16021 tactgggatc catgcattta aagacttgga ggcggggttg cgaaatatct ttggcggccg
    16081 ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa gaagcacttg ccgaaatggc
    16141 cgaacgtgca aaagcattag gagctgatgc gatcattggc gtaaaaatgg actatgaagt
    16201 attaggcagc gacaatggca tgctgatggt cacatgtagt ggaacagctg tctccacgag
    16261 ataagtaggg gacatctgtt ttaataacta aagagagatg ggacatgtag tggcactcaa
    16321 gaattagatt tttcagtcta acttcgaggt actctacctt cccatcctct cttttttatt
    16381 tctttttgcg gatgattccg taagctgctg cgactaacac acccgcccct gctaaggaat
    16441 aactgacaga agctgttttt tcatttgttt ttggcaaagt ctgacttgtt gatttggatg
    16501 gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga ggtcactgat ttttctcctg
    16561 ccgaatgcaa aatatctttt attcgttgga aactagctgc taagtcagct ttttttgctt
    16621 caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc gtggattgct tgactggccg
    16681 ctgtgatatc cgcttgagtt tgacttagct ttccagaagc tgtttgagca acctgattta
    16741 attgctgatt gaattctttt gcttgttgga tcttcccgtc aagatctgtt tggacagctt
    16801 ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg tgcacggtca tttcccactt
    16861 tatttttaag atcagctaaa tcttgatcga ttgctgcttg gtcttctttc aatttgtttt
    16921 ttgcagcttc taatgcttct tcgagttctt ttcttttatt ttccaagtct tgcaaatgat
    16981 cgatttggtc agaagaggta gaactattta ttgatgtatc ttctgaagat gaaatcgttt
    17041 ctcctgaatt atctgtcggt acattggaag tcatttctgt ttccgaggca ccgttttcag
    17101 caccagaaga gcttatagtt gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa
    17161 aagctgtctg tcccaataag aaaatactgg aaacagcaat tggccagatt aacggcttca
    17221 ttcttttttt catcactttt cccatcctta ctacttttta actaatttct tattataagc
    17281 aaaaatgttt ggaagaacca taaacacaac aggctgtttg tctaaaaaac ggtgcatctt
    17341 ttattttttt gtacgttccg ggcttttcca tgtcccgctt ccataaccta aaatcgtttt
    17401 gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc actatccaga acaaaagcat
    17461 atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt cgatcatcta caatcagcga
    17521 agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt
    17581 aaaagtaata taaatctgtt cgtagtttcc ttgatataca taatgcccga ttaatccagc
    17641 aaaaagaact acggaaatac aaaagaatat tgaccagaca atactcaatg tttgatcaat
    17701 gaagatgatc gttcttccaa tatgtttaaa aggatggagc atcacttttt tgaaattcgt
    17761 cagccagact tctgttccac cttttgccca acgttttctt tgtcgatata gcatcttcaa
    17821 cgtcacaggt acttccatga aaaagataat ctcaggagca aaaacagata accagtcatt
    17881 taactgatga tcccaagcaa tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc
    17941 cacatcgatc aaggcttctt ttcgatacat cgtatttgct ccactgtacg cataaagacc
    18001 accgaaaaca gctgactgtg ttcgtttaat gatgcccacg atacttgaga attcaaccgt
    18061 ttgagatttt gcaatcaatt tggtacgatt ttggacatcc atatttgcag tgaccgcagc
    18121 aatatgtctt gcaccagggc gaatgaaata attcacatac ctaatcagtg catctggctc
    18181 cggtaccgtg tctgcatcat tgcttaaaat caatttccct tttgcaaagg caagtccgat
    18241 attaaacgcg tgggcttttc ctttattttt ttcgatccga accacacgaa gattagcgta
    18301 ttttttcatc agtctagcta atatttctgg tgtttggtcc gttgaaccat cgtctgtcac
    18361 taatacctca tagttgtgat aattcaattt cgtcatcaaa tactcgattg tatcttcgat
    18421 cacaatttct tcattatggg ctggcaccat gatcgtgatg aacggttcga cactcaaggg
    18481 aacatctacc cattcttttt gcttatgttt gtataaaaac acataacacc agacaccaat
    18541 aaaccataca aatccgccta aaatcggata agaaacgaga aaaaatacat aatagacaaa
    18601 caagtatcta agaataaatt acctgtatgg gtgattgaga tcatttcagt tccaccccat
    18661 tttctttata caaatctgca atgaatgtcg tatctagatt tttctcttcg gagacagaat
    18721 aaaaacggac cgtttctctc tcttcttttg ttccaaaacg ttccgtatac acttcttcaa
    18781 gtaattcttg tctttttttc aatttttcat cttcatattc aaaagattta tttacttttt
    18841 tgtatagatt tcgattgttc cacctagtca atacaaatga ggtaatcagg aaactgaaaa
    18901 aaatcaatac aaaaaagccg attaagaaat acagcatttt cttttctgat aaaaaagcgt
    18961 acaaggaaaa atccttggct ctttctggtc tcgttaatgg gaaaaagacc catataaaag
    19021 gaagaacaac gatcatccat cctaaaagag ccactaacgt ttgccaaatc tttaaccagt
    19081 aattaccttt tgaaaaatac ttatctttca ccaaaattga tgcaacttct tctcttgtta
    19141 cttttttcat atcctcacct cacgtttttt tgttacgacg taggagttta ccatacattt
    19201 catttctttt cacataaagt gttcctaaat ttttcctaag aaaaagaaca aaacatttct
    19261 catcagcaaa tgaaaaatat tttgttcttt ttatcagttg ttctttttat tttttatctc
    19321 ttaattatgt tgctctttcc aatacgacaa tgcttttcat tcctctagat atcgccgtgt
    19381 acatttgtct gcgtcttctt tgctcatctc catcgttttc aaatggatat aagattacat
    19441 gatcaaattc cagtcctttt gctagactca aaggaatgat ttggcagttt tctaatccat
    19501 atgaattttt tttaatcgat tcttgtagcg tttgggtttc ctcttctgtt tgggtgataa
    19561 ctgccgcttt ttctcctttt agagaaacca aaatctcagc tagtattttc aacaattctt
    19621 cagaggatcg aaaagagcgg tattcgggtt cttttccttg cggtcggatc gatacgatgt
    19681 cgatcttctc ttgatccaca gcataggttt tgaaaagctc tgtgattgct ccgctagaac
    19741 ggtaactatt tctaagatca taccgatgga tcggcagatg gtagtcgtcg aaacaacgca
    19801 taatatcagc aaaagtagtg gaagaattaa agattgcctg attctcgtca cctactaaag
    19861 taaagtgagt ttttggaaat aattcgatca atagacctaa ttgtgccagg gtataatctt
    19921 gtacttcatc aatcaataga tagcgaaatg ctggtacatt taccttttca acaagtaaat
    19981 gacgaatagc taatataatg actgcttcat caacagtacg tgtgggttgc caagcataag
    20041 ctgagccata tcgtttttca taaattcttt caaacaactg atgctcatcc acccatgcca
    20101 tttcttcgat ttgtctggtt atttttcgat attttttttg taaaagtttc cttgcataag
    20161 ctacaatact ttgttccgaa tcatcagaaa tcaatttacc gaataattcc aactgcctat
    20221 cttctgtcaa cgagagtacc tgattctgca cagcaggact ttttgcttgt ttcaaaagat
    20281 gattcttcca ttcgctcatc agtgcctttt tcgttgcttg aagcctatcg atcaatttag
    20341 gatgagcagg aactttctca tatatttttt cgatcgtttt tttagaaagg actgtttttc
    20401 catttttagt aagtccacgg ataaaattcg gatgatctaa aaacaaagga tctgattgtt
    20461 ttagattatc gataaacttt ttgctacgca aacgttctgt ctgctccgat acattttctc
    20521 ccgtgatacg tttgaaataa gcctcttcac cttctatttc ctctgctgaa agctgtgaaa
    20581 ccagctgtat gatggttaga ttcagtggat ttctttcacc taatgatggt aggacattcg
    20641 aaatatagtc gataaaccga tgattaggtg atagaatcag caagtcatcg gatgtcatcg
    20701 tatctcgata taaatagagc agatacgcaa tacgctgcat gatagcagag gttttaccac
    20761 ttcctgctac gccatttacc aagatatttt gacttttcac atcccgaatg attgtgtttt
    20821 gttccgattg gatcgtagca gtgatagcct tcatttcatt ggtctcatct tggcctaaga
    20881 cttctaataa tacgtcatct tgaatagcta cagacgtatc aaaaaacttg accaaacgat
    20941 ctctttccaa aagaaattgt cgtctttctt taataaccgt atcgatttta ttttgacgaa
    21001 ccgtataaga agatggacca atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc
    21061 aatcataaac aagattttca tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg
    21121 attcttttgg ctcattatcc agaaagtcta caacgacttt tccaaagtag ggacttttca
    21181 ataaacgttc gatctttgct agctgatttt ctgcgttttt ctgtttgata ttcatctgat
    21241 cgatttctct atttttcatt tcaatcatcg aaaaagtatc caggttatct aagtaactgt
    21301 caaaattcaa tcgaagctcg ccagccattt ctttcaacga ctggtttcct tctatttttg
    21361 ccgtatccat cgattcccgc agttcgtttt ttttatcaat tagcttttga tacacgttct
    21421 tcagatatgc ttcttctaac tgtttttgtg tgttgaccat acacattctc ctcttttttt
    21481 aaagtggtgg aaccgattag tttacctctt ttaagagtcg aatgcaagct ttttaacgag
    21541 tatcatttgt taggatatat cagttcttca ggtcggacag tttgatataa agaaaatgca
    21601 ccatcaaggt tttttacgtg gtagccagac tgcttcaaga ttctttccgc tagatagcta
    21661 cgtaatccgc tatgacagct gacgatatat tcttgttggc tgtctaattc atttaatcgg
    21721 tcccgaagtt cattcaaagg gatatgtttg gcttccttga atttgccctt ttccaattct
    21781 tctgctgttc gcacatctaa taaaattttt ccttttgcta gttctgtcgg caattcatgc
    21841 cattgtacgg tttcactaag cccttctgca atgttcattg ctgcatatcc tagcatgttt
    21901 actggatctt tggctgagcc aaatggcggt gcatacgtca attctaattc cggtaaatcg
    21961 aagatcgtca aatgcccttt gattgctgtt gctaaaatat cgatccgttt atccacacct
    22021 tttgccccaa caccttgtgc accataaatc tcgccagtct ctggatggaa gatcaacttc
    22081 agtaaaatat ctgttgcttc tggataatag cttgcatgat cttttcctga tacatgaatg
    22141 acttcaaaag acagttcgtt ttctttggct gttctttcac ttaaaccggt ggatgcggca
    22201 gtcattccaa acgttcgtat aattgctgtt ccaataccgc cttgatttct tcgagaaatt
    22261 ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg ctggtgaagc aagagaaata
    22321 agtgcatctt ttccagtgat ttgctgtttt acaacgatag catcccccac cgcataaata
    22381 tcaggaatat tcgtttgata acgttcgtct actaggatac ccccgcgtat gcctaattcg
    22441 attccagcgg tcactgctag ctggttatca ggacgaacac caatggataa aataactaaa
    22501 tcagcaggga tggtacgccc atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca
    22561 gcgacagcat gagaagtaat cacttctact ccttgatgag acaattcttt ttctataaaa
    22621 gctgccattt cttcatccag tgttggaaga atatgtggtg cttgttcgat caccatgact
    22681 tctaaacctc ttctttttag ggtttctgcc atttccaatc cgatgaatcc tgcaccaacg
    22741 atcacggcac gctttggctg tttttctaat gcatgcatca ctttatcgat atctggtaca
    22801 tttctaatag aaaaaacatt gtcggcttct gttatccctg gaatcgatgg aacaactggt
    22861 tttgcaccag gagataaaac caatgcatcg tactgttcaa tgtgtttttg accctcatgt
    22921 acgacttcta ccgttttgtt ttcgggaaag atggctgtca cttcatgatt tggacgcaca
    22981 tctaaacaaa aacgagcgga taatgattct ggcgtttgga caagaagatt ttcccgttca
    23041 ctgatttctc ccgaaagata ataaggcaag ccgcaatttg caaaagaaac atacggtcct
    23101 ttttcaaaaa caacgatttc tgcatcttcc attaatcggc gaagccgtgt agcagcagac
    23161 attccacctg ctacacctcc gacaataacg attttcatct tatttccctc ctactgttcg
    23221 atcaaaccat tgattcatgc caccacgtac attcactaca tcataaccag atttctttaa
    23281 ttctttagct gcttgtttac tcctcatccc tgattgacaa atcacataga cggtcttttc
    23341 tttgtctcca tcatatctgt ttatactttg taatggaaca tttttagctc cttttatatg
    23401 cccgccccgg tattctgcag gtgttctaac atcaagtaac gctggatttt ccaaaagctt
    23461 ttcctttaat tctgggactg ttatcgattt tattgattga aatagcaaat tcattttctg
    23521 cacctcattt acatttttat aaatacctca tggggtatat taaacaggag ctttattaat
    23581 atgtcaacca gaatgactct tcattttttt cactactttt tatatttgca taaaataccg
    23641 ataaggggta ttatctaatt ataaaaatag aggagaatgt gttatggaga ttgataataa
    23701 aaatatcctg aaccgtttga aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat
    23761 cgaagacgaa aaagaatgta tggatgtcat cactcaatta agcgctgttc gttctagtat
    23821 tgatcgcgta atgggcatga tcgttgcaga taatctgaaa aattgctttg agaaacctga
    23881 tagtaatcca gaagaacaag cggctaaact agaacaagcg atcaagatga ttattaaaaa
    23941 gtaaaggaat gatttcaatg aaagtcgttt tccatattga tgaattagaa aaatggtcag
    24001 aaacagggaa aaacgtaaaa aacctgataa aagcttctcc agaaacgacg attgtcgtat
    24061 ctgtaaatgg catcgcgatt accggttatc tagactctgc caatgcagaa ttcttagatt
    24121 tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg tgctaatcat atttcagaaa
    24181 gttctttacc tgaacaagta atcgttgtac cagcaggtgt ccttgattta gttgaactgc
    24241 aatcccaagg ttatgcttat atcaagcctt agtcagtaga ggttgcgaca gaagtgatca
    24301 gcttcaagaa atttcggctt atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa
    24361 ggttgtgaaa a
//
LOCUS       DACEPW010000024        24012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEPW010000024 DACEPW010000000
VERSION     DACEPW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.024"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(28..726)
                     /locus_tag="IW819_000750"
     CDS             complement(28..726)
                     /locus_tag="IW819_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1243875.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="IW819_000751"
     CDS             complement(719..2071)
                     /locus_tag="IW819_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1243876.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="IW819_000752"
     CDS             complement(2294..3694)
                     /locus_tag="IW819_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1243877.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="IW819_000753"
     CDS             3855..4592
                     /locus_tag="IW819_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1243878.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="IW819_000754"
     CDS             4717..5196
                     /locus_tag="IW819_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1243879.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="IW819_000755"
     CDS             5193..5960
                     /locus_tag="IW819_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243880.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="IW819_000756"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="IW819_000756"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1243881.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="IW819_000757"
     CDS             complement(6863..7465)
                     /locus_tag="IW819_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1243882.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="IW819_000758"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="IW819_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="IW819_000759"
     CDS             8146..8730
                     /locus_tag="IW819_000759"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1243883.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="IW819_000760"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="IW819_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1243884.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="IW819_000761"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="IW819_000761"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1243885.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="IW819_000762"
     CDS             10631..10810
                     /locus_tag="IW819_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243886.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="IW819_000763"
     CDS             10832..11857
                     /locus_tag="IW819_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1243887.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="IW819_000764"
     CDS             12032..13276
                     /locus_tag="IW819_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1243888.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="IW819_000765"
     CDS             13429..14376
                     /locus_tag="IW819_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1243889.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="IW819_000766"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="IW819_000766"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1243890.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="IW819_000767"
     CDS             complement(15278..15520)
                     /locus_tag="IW819_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243891.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="IW819_000768"
     tRNA            15641..15713
                     /locus_tag="IW819_000768"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="IW819_000769"
     CDS             complement(15832..16470)
                     /locus_tag="IW819_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1243892.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="IW819_000770"
     CDS             complement(16593..17225)
                     /locus_tag="IW819_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1243893.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17576..17875
                     /locus_tag="IW819_000771"
     CDS             17576..17875
                     /locus_tag="IW819_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1243894.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18370..20535
                     /gene="acm"
                     /locus_tag="IW819_000772"
     CDS             18370..20535
                     /gene="acm"
                     /locus_tag="IW819_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1243895.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20807..21292)
                     /locus_tag="IW819_000773"
     CDS             complement(20807..21292)
                     /locus_tag="IW819_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1243896.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21772..22413
                     /locus_tag="IW819_000774"
     CDS             21772..22413
                     /locus_tag="IW819_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1243897.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22432..23067
                     /locus_tag="IW819_000775"
     CDS             22432..23067
                     /locus_tag="IW819_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1243898.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23085..23900
                     /locus_tag="IW819_000776"
     CDS             23085..23900
                     /locus_tag="IW819_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1243899.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         8311 a         4086 c         4772 g         6843 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
    17341 gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt
    17401 agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata
    17461 acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt
    17521 atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc
    17581 taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga
    17641 gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca
    17701 gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg
    17761 agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga
    17821 aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg
    17881 agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat
    17941 ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat
    18001 tgtattttat cctttaatta acacaaaaac agctagtata aactaataat gttaattaaa
    18061 agtgtatttt atgaaactat cgttatttat taatgttgca tttttgtaag ttaaaaattg
    18121 gaaatataga ttgaaaaaat gaaagcaata agtaaaataa aatgaatata tgcgaaaaaa
    18181 taatttacat taggaaaaac cttgtaaaaa cagttttttg gcatatttat tttttataaa
    18241 tatttgtaaa ataactatca attttttagt taaaagattg gaaatgagtg ggggaggaat
    18301 tgaagaaagg atttttgaga gatgatatag taggttttat ttttagttaa aataggggag
    18361 atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat
    18421 ataacatcac tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat
    18481 gtcacttcgt tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa
    18541 ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg
    18601 ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa
    18661 aataaaaatg ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa
    18721 aatattaaaa acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac
    18781 gtaaccgata ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca
    18841 gctactgtaa atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa
    18901 acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat
    18961 aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga
    19021 ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga
    19081 ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca
    19141 gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata
    19201 atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg
    19261 gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca
    19321 gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt
    19381 ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga
    19441 gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga
    19501 gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc
    19561 aattggattg attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat
    19621 gatacagagg gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca
    19681 actaaaattt ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga
    19741 gcaagtacaa acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga
    19801 ataacttcag tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg
    19861 tttaaagtgc aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa
    19921 attgaaaacg ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa
    19981 aaaacatggg aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat
    20041 ctattagccg acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc
    20101 tatcgtttca caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg
    20161 gaagacaacg tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat
    20221 acaccggaaa aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt
    20281 ccagagaaga cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt
    20341 gctcatacag gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct
    20401 aaacagataa caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt
    20461 tttgctgtaa tcattggttt attgatagta actatctgtg ctgggatatt cttacaaaaa
    20521 tataaaaaag tttagttaga atataatatt ttttgttaaa tatgactgaa atcaaaagaa
    20581 taaaattccg atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag
    20641 aatcaataaa tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc
    20701 atactagttg ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat
    20761 aaagaaaaag acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat
    20821 aaactggcag atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca
    20881 acgtagtggt attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat
    20941 cgatcacgac ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac
    21001 ctgctggaac gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt
    21061 cttttaattg ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa
    21121 gatagatttg ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta
    21181 gggaaaaagg gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga
    21241 tacccagttt ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc
    21301 accctccttt ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt
    21361 aaattacacg taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt
    21421 tagagagtct ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc
    21481 gattttctga agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct
    21541 agatgaccag ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt
    21601 gattaacgtc ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga
    21661 ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt
    21721 cctcttggca tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat
    21781 ttgattgaag ttatgccgca attattagta ggagcactca caacattgaa attattttta
    21841 tttaccttga ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat
    21901 tttaagccgc tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg
    21961 ttgttgcaac tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt
    22021 ttcgaagcag cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt
    22081 cgtggaggga tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta
    22141 agtccctttc aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct
    22201 tctgtaggga atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt
    22261 ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct
    22321 ttgctaggcg tggcaatcat ctatcttaca ttgactggta ttctaacaat cataatgaaa
    22381 caagttgaag gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa
    22441 ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc
    22501 attccagatg ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt
    22561 ttacgtatct tggctggctt agaaagttct gacagcggag aattttattt agacggtgta
    22621 ccgttcaacc cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac
    22681 ttccagctat ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta
    22741 ttgaaacaga caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg
    22801 attagtgatt tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta
    22861 gcgattgcca gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt
    22921 gctttagatc ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat
    22981 ggggtaaccc aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa
    23041 ttactaaaag tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt
    23101 ttctattcgc tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt
    23161 cagcagataa ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata
    23221 catttgttcc tatgggattt agagataaag acgggaagct cacaggattt gatattgatt
    23281 tagccaaagc agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt
    23341 caatgaagga atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca
    23401 aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag
    23461 tgctgatcac acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat
    23521 taggtgcgca aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa
    23581 aagagatagt caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg
    23641 atctgaaaag cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt
    23701 tgaaacaaag aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg
    23761 attttgcagt tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc
    23821 tagatcaact agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag
    23881 atgttacgcc gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt
    23941 tttccgaata aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct
    24001 cacaacctct tg
//
LOCUS       DACEPW010000025        23653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEPW010000025 DACEPW010000000
VERSION     DACEPW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.025"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            251..526
                     /locus_tag="IW819_000777"
     CDS             251..526
                     /locus_tag="IW819_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243900.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            539..2617
                     /locus_tag="IW819_000778"
     CDS             539..2617
                     /locus_tag="IW819_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1243901.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2777..4663
                     /locus_tag="IW819_000779"
     CDS             2777..4663
                     /locus_tag="IW819_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1243902.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQVDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4675..5622
                     /locus_tag="IW819_000780"
     CDS             4675..5622
                     /locus_tag="IW819_000780"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1243903.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5642..6169
                     /locus_tag="IW819_000781"
     CDS             5642..6169
                     /locus_tag="IW819_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1243904.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6232..6885)
                     /locus_tag="IW819_000782"
     CDS             complement(6232..6885)
                     /locus_tag="IW819_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1243905.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7017..7859
                     /locus_tag="IW819_000783"
     CDS             7017..7859
                     /locus_tag="IW819_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1243906.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8017..8913
                     /locus_tag="IW819_000784"
     CDS             8017..8913
                     /locus_tag="IW819_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1243907.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8916..9629
                     /locus_tag="IW819_000785"
     CDS             8916..9629
                     /locus_tag="IW819_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1243908.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9649..10167
                     /gene="msrA"
                     /locus_tag="IW819_000786"
     CDS             9649..10167
                     /gene="msrA"
                     /locus_tag="IW819_000786"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1243909.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10164..10391
                     /locus_tag="IW819_000787"
     CDS             10164..10391
                     /locus_tag="IW819_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1243910.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10543..11094
                     /gene="lepB"
                     /locus_tag="IW819_000788"
     CDS             10543..11094
                     /gene="lepB"
                     /locus_tag="IW819_000788"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1243911.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11177..11719)
                     /locus_tag="IW819_000789"
     CDS             complement(11177..11719)
                     /locus_tag="IW819_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1243912.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11956..12354)
                     /locus_tag="IW819_000790"
     CDS             complement(11956..12354)
                     /locus_tag="IW819_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1243913.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12614..13474
                     /gene="ylqF"
                     /locus_tag="IW819_000791"
     CDS             12614..13474
                     /gene="ylqF"
                     /locus_tag="IW819_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1243914.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13467..14234
                     /locus_tag="IW819_000792"
     CDS             13467..14234
                     /locus_tag="IW819_000792"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1243915.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14290..15147
                     /gene="dprA"
                     /locus_tag="IW819_000793"
     CDS             14290..15147
                     /gene="dprA"
                     /locus_tag="IW819_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1243916.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15269..17347
                     /gene="topA"
                     /locus_tag="IW819_000794"
     CDS             15269..17347
                     /gene="topA"
                     /locus_tag="IW819_000794"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1243917.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17316..18692
                     /gene="trmFO"
                     /locus_tag="IW819_000795"
     CDS             17316..18692
                     /gene="trmFO"
                     /locus_tag="IW819_000795"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1243918.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18890..19795
                     /gene="xerC"
                     /locus_tag="IW819_000796"
     CDS             18890..19795
                     /gene="xerC"
                     /locus_tag="IW819_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1243919.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19832..20380
                     /gene="hslV"
                     /locus_tag="IW819_000797"
     CDS             19832..20380
                     /gene="hslV"
                     /locus_tag="IW819_000797"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1243920.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20394..21794
                     /gene="hslU"
                     /locus_tag="IW819_000798"
     CDS             20394..21794
                     /gene="hslU"
                     /locus_tag="IW819_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1243921.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21812..22603
                     /gene="codY"
                     /locus_tag="IW819_000799"
     CDS             21812..22603
                     /gene="codY"
                     /locus_tag="IW819_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1243922.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22685..23560
                     /locus_tag="IW819_000800"
     CDS             22685..23560
                     /locus_tag="IW819_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1243923.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8191 a         4079 c         4982 g         6401 t
ORIGIN      
        1 tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa
       61 catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg
      121 gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac
      181 aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga
      241 gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg
      301 cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt
      361 gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt
      421 acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa
      481 gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat
      541 ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag
      601 ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg
      661 tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat
      721 tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt
      781 cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc
      841 gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc
      901 gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa
      961 aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc
     1021 cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat
     1081 gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa
     1141 acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct
     1201 gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg
     1261 aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt
     1321 gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact
     1381 gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg
     1441 gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat
     1501 gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa
     1561 aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac
     1621 agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca
     1681 gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat
     1741 cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt
     1801 gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa
     1861 tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga
     1921 tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact
     1981 gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc
     2041 taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca
     2101 gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct
     2161 ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga
     2221 agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt
     2281 agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa
     2341 acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga
     2401 tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga
     2461 atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa
     2521 tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt
     2581 tcgagcaaag tcaaaaaaaa cgagtccaat aaaataaaaa ggtaaaaatt ttcaagtttt
     2641 ataaaaaaca aaacatatca gaacaggatc gaatcggtcc tgttcttttt ttgagaacac
     2701 aaaaagaaca aataccaaaa aaattttcct gaagttcact atttttatga tacaatatcc
     2761 aagataagga gggaacatgt tgaaagaatt aaaagtaact gacctcaaaa aaacttatgg
     2821 tgaaaaagat ttgtttgacc agatttcttt tttggtccac gaaaaagacc gtatcgggtt
     2881 gattgggacg aacgggacgg gtaaaacaag tctcttaaat attattgccg gtatcgatag
     2941 cagcgacggt gatcggcaaa cagtgtttta tccaactgat taccgtattg gttatctttc
     3001 tcaagcagct gaattttccg atgaattaac tgttctccaa gcagtctttc aaggaaacag
     3061 ccccttgatc caaactgttc gagcatatga ggaagcattg atcgaactag gggaaaacgg
     3121 agaagaccct gatgtgcaaa aacgttatgc taaagcagaa gaacaaatga ataaagaaga
     3181 tgcttggact acagatacca acgcaaagat tattttgcaa aaattaggaa tcagtgaatt
     3241 agataaaaaa atcagtactt tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt
     3301 gttgattgat gaacccgatt tactattact agacgaacca acaaaccatt tagattatga
     3361 agcgatcgag tggttggaaa actatttgaa acaataccgt ggcgcactat tgatggttac
     3421 ccacgatcgt tattttctag atagagtagc aaaccgaatc ttcgaacttt catttggaaa
     3481 attatacgag tataaaggaa attatgaagc ttacgtctta gaaaaagcag aacgtgatcg
     3541 tgtagaagta gaacaagaag aaaagcgcaa gcggctttat aaacaggaat tagcttggat
     3601 gagggctggc gtacaggcac gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga
     3661 tctaaaggag aacttgtacc aagtaaacca agtagatgat ttagagctga acctagcgac
     3721 acaacgttta gggaaaaaag tactggaaat caaagatggc agttaccgta taaatgacca
     3781 aacactgcta gaacatttag atttattgat tcaatctcgg gaaagactag gtattactgg
     3841 taaaaatggt gcgggaaaat cgacattgct taatattcta gctggcagaa ttcccctgga
     3901 tagcggtaca atgtctatcg gtgaaacggt tcatcttgcg tattatacac aagaaaatga
     3961 agaaatggca ccagataaac ggatgattgc ttacttacaa gaagctgctg aagaagcaaa
     4021 aacagcagat ggcagtcaaa tcggagtagc agaattactt gaacgatttt tatttccaag
     4081 atttatgcat gggacactga taggcaaact ttcaggcgga gaaaaacgcc ggttattttt
     4141 gttgaaactg ctgattcagc aacctaatgt tttattattg gatgaaccga ccaatgattt
     4201 ggacatagca acattgacga ttttggaaga ttattttaga agttttccag gtgctgtgat
     4261 cactgtttcc catgaccgtt atttcttgga caaggtagca gataagttat tagtattcca
     4321 aggaaacgga aaacaagaac tttattacgg aaacatgtcc agttatttac tgaagcaaaa
     4381 agagacccag cagcctgctg aaaaagcgaa accaaaaata cagagcaagg aacctgctga
     4441 aaagaagaaa ttatcttaca tggaacaaaa agaatgggaa acgattgaag atgagattgc
     4501 agaacttgaa gaaaaaatct cattgctcca agaagagatg aatcatcaag gagataattt
     4561 cactcgatta caagaattgc aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa
     4621 aatggcacgg tgggaatact tgagtgaatg ggtagaagat taggaggaaa aaggatggaa
     4681 caagcgtatc ttgacttagg aagaaaattg ttggaagaag ggcatcgtaa agaagacaga
     4741 acaggcacag gcacattaag cttattcgga taccaaatgc ggtttgatct gcaaaaaggg
     4801 tttcctttgt taacaacgaa acgagtacct tttggattga tcaaaagcga attgctttgg
     4861 tttttaaaag gagatacgaa tatccgctat ctattacaaa ataaaaacca tatttgggat
     4921 gaatgggcat ttgaacgcta catcaaaagt ccagactata atggaccaga tatgactgat
     4981 tttggccata gatgtttgac agatcctgaa ttcaatgaag aatataaaaa gcagagcaag
     5041 cttttttgcg agaagatcct aaccgatgat tcctttgctg aaacatacgg cgatcttgga
     5101 cacatctacg gctatcaatg gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa
     5161 atcaaacaag tgatcgaagc aatcaaaaag actcctgatt ctagaagatt gatcgtttct
     5221 gcatggaatc cggaagatgt tcctagcatg gctttgcctc cttgtcatac gatgtttcaa
     5281 ttttatgttc aagagggacg gctaagctgc caattgtatc aacgcagtgg ggatgtgttt
     5341 ctaggcgttc cgttcaacat tgcaagttat gcgttgctga ctcacctaat tgcacatgaa
     5401 acaggtttag cggttggcga atttgttcac acattaggag atgctcatct ttatatcaat
     5461 catatcgaac aaatgaaaga acaattgagc cgagaaatac gcgcatttcc gactttagtt
     5521 ttgaatcagg aaaagcagtc agtatttgat tttgagatgg aagatatcca attagaagga
     5581 tatgacccac atccggcgat caaagctcct atcgctgttt agaatcagaa aggatgagaa
     5641 aatgttagct gcaatttggg cacaggatga aaacggattg attggaaaag aagaccgtct
     5701 tccttggcgt ttgccaaatg atctgaaatt cttcaaacaa atgacagaag caaatacact
     5761 agtgatggga cgaaaaacat tcgaagggat gggtagccgt ccgttgccga atcgtcagac
     5821 gattgttttg acgagagatt cttcctataa agcagaaggc gtgcatgtca tgcatagtgt
     5881 ggaagacgtg cttgctctgg aaaaagaaac agacggtatc tttttcatcg ctggagggtc
     5941 agctgtttat caagaatttc tgccattttg tacgatatta taccgtacag tcatccatca
     6001 tgtattcgat ggagatgctt attttccacc tgttgactgg tctgattggt cactgatcaa
     6061 tactagtcaa ggagaaatgg atgaaaaaaa tcgttatccg catcaatttg aaacgtatca
     6121 gcgtaatgag aatcctgtaa aaagcgggac aggcgaattg acgatataaa aaagctatgg
     6181 atagactagc tgttatttag agctgtccca tccatagctt tatttttttt attaagtaaa
     6241 gaataaaaca gagaagtaca taaaacctgc acctaacata acaaacaaat gccaaacaac
     6301 atgcatatag cggatagaat tcaagctata aaagaatgcg cctatcgtgt aagaaagccc
     6361 gcctgctacc atcaaaataa caccagtcat tccaagagag tgataaagag gaacagcagc
     6421 cacgacacag agccacccca taaaaatata gatgatcgta gatacttttg atactctctc
     6481 ccgtttatgc aacgtcaacg ctttatatat aatgccaaga atcgcaaaca accagattaa
     6541 tacaaataaa gtccaaccta gccatcctcg gatactcagc aaacaaaaag gagtataact
     6601 tcctgcaatc aaaagaaaaa tcgaactatg atcgaatact tggaacacct tttttgcttt
     6661 tgtaaaaatc aatgaatgaa acaaagtaga cgttaaaaat aaaagaatca tcatcgatcc
     6721 atagatagca taagagacaa tatggaggga ggaatgcaag tgggcacctt ttactaaaag
     6781 aaccactagt cccgcgatac tcaatccagt cccaattcca tgggtgactg cattaaacac
     6841 ttcatttaat atttcatacg ttcgattaaa tttcgttttt tccatgaaaa cggctccttt
     6901 actaggtgta attaatgaaa aatcattatt tgtctgttat actaaaaata gaaattgtct
     6961 tacatgtagt ttaacatgaa taaggcatct tgttaaagaa agagagagag aaaataatga
     7021 caaatgtaaa aatcgtaaca gattcttcat gtacgatgga aaaaaatgta cgcgatgctt
     7081 taggcattcg tgtcatgccg ctatccatca tgatcgacgg aacagtatat cccgatgatg
     7141 accaactaga cggcgaaaga tttatggaaa tgatggcgaa agcaaaaaat ctgcccaaaa
     7201 caagccaacc accaattgga gaattcatct cgatgtatga tgagctagga gaagacggca
     7261 gtgaagtgat ttcgatccat atgacaaaag gcttgagtgg aacagtagaa gcagcaagac
     7321 aagccagtaa tctaacaaaa acgaaagtaa cggttatcga cagtgatacc actgaccaag
     7381 gattatcttt tcaggtaatt cgagcagctg aattagcaaa agaaggtaaa acagtagaga
     7441 aaatccttga agaagtcaaa aaagtacaag acaatacaaa attatacatc ggaatatcca
     7501 cactagataa cttggtaaaa ggtggaagaa tcagccgaac aactggtttg ctgtctagtg
     7561 ttttgaacat acgcgtggtg atgaatttcg cccattctga attaatccct gtaactaagg
     7621 ggcgaggaaa gaaaacgttc gataaatggt ttaataattt gaaaaatgaa ctgaaagaat
     7681 tgccaaatgt gcgacaaatc ggtatttcac atgcggatgc aaaagaattg gcgcaagaat
     7741 tcaaagaggg tttgcaagaa ttattcccgg acatgcatat ccctgtactg catacgaatc
     7801 cggttatcgc tacgcatact ggtaaagacg cgtttgcaat catgtattat gtagattagt
     7861 cgaaaagagg ataagccctc agcctgaaag gcttgcagct tattctcttt ttctttataa
     7921 aattaggaaa ataattgttt tcttttttca taaagactac ctttttgata aaatttttga
     7981 taacattaga accaatcgaa tacaagaggt gaaaaaatga aattaacgaa acgacatttg
     8041 atcctcgttt tgttcccatt attaattgcg atcatcatct atgcaggttt agctgcttct
     8101 ataccgaaag ctggcccact aatgaaacca gtaactactg tgagagaaga tccaaataaa
     8161 aaagaaaaac ttcactacat agctatcgga gattcgctga ctgaaggagt aggagatcaa
     8221 accaagcaag gaggattcgt tccccttgtc gcaaatgatc taaaagaccg ttataatttg
     8281 accgcggttg aaacagaaaa ctatggtgta aatggtgaac gaagtgacca gatcttaaag
     8341 cgagtaaaaa caaaagaaga aattcgaaac aatatcaaaa cagctgattt tatcacactg
     8401 actgttggcg gtaatgattt gatgaaagtt atccaaaata atttattcgg gttaacggtc
     8461 aattccttta aaaagccact gaaaaactat caggaaaatg tcacagaact attggaagaa
     8521 tttcgatccc tcaataaaac cgcacctatt tacattttag gcatctacaa tccattttat
     8581 ttgaactttc ctgagatcac tgacatgcag acaattgtta acaattggaa caaaggaaca
     8641 gaaacggtga caaaaaaaat gccgaactgc tactttatcc caatcaatga tttgctttat
     8701 caaggattga atcaggaagt aggtgtcaca gaagaaggaa cagatcaaac aactagcacg
     8761 acagaaaatc taaatggcat caaaaataat gtgctctatg aagaagatca tttccatcca
     8821 aataatttag gttatcaatt gatggcaaat gcagtgagag atcagctgat cgagacacaa
     8881 aaccaatggt tagagaagga ggatgatcaa taaacatgaa aacaagatct gacgtatctt
     8941 caaacaaaac aaagaaaaca aattactgga aattcgcctt ccttatcctt cttggggtag
     9001 tacttggttc gaccgtattt ttaggcagcc gtatttttgc taatcgggaa ccagaactcc
     9061 cagaaatacc agctcttacg gaaagacaag gagatcctgt cttaacgatc aattcaaata
     9121 aagaaaaagt caatcaaatc atcagctttt tccttagtga gtatcaaaaa gatagtgata
     9181 ttgaatataa attttatttg gaaaatgaag cactactcaa tggtactttc gaagtactag
     9241 gttttcctat cgatttctat ttatattttg atccgtatgt gatggaaaac ggaaatgtcc
     9301 aactaaaagc tagaagtctt tctatcggaa cattgagttt gccaatcaaa gatgtgatga
     9361 atatgatcaa gagaaactat aaattaccag attggatcga agtcaataca gatgatctga
     9421 ctatcatgct taggttggat caattccgaa tgcaaaacgg gatgtatatt aaagcagata
     9481 aaatcgactt agtaaatgat gacattcggt ttagtctata tttgccaaaa gatacctctg
     9541 atgcttcaaa caactcaaat accaacaaaa ctagcaccag cagtactgaa agcaagacaa
     9601 aagaaaataa aaaatcatcg gatgagtagt attagagggg gaatcataat ggaacgagca
     9661 atatttgcag gaggctgctt ctggtgtatg atccagcctt tcgacacgct tcctggtatc
     9721 catacagtga tgtcaggata tactggaggg catgtaccag atccgactta tgaacaagta
     9781 aaaagccaaa cgactggaca tacagaagca gtggagatta tctatgatcc aacattgatt
     9841 tcatatgaac aattagtaga actttactgg cagcaaactg atccaactga cgctttcgga
     9901 caattcgagg atcgcggtga caattatcgc ccggtgatct tttatttgac agaagaacag
     9961 cggatcattg ctgaaaaaag taagaaacag ttagctgaaa gtggacgttt cgaacagcca
    10021 atcgttacaa aaatcgaacc agcccaacca ttttatctag cagaagaaga ccatcaagag
    10081 tattataaaa agcatcctga aaattttaaa agaaaccatg cacgccgggc tgcctttatc
    10141 gaagaaaatt ggaggaacga agggtgaatc gaagcttttt tcattattta atgacactgc
    10201 gaggcggtgg cacacatgat gctttatctg ttttcgctac agaagccggt aaagacatcc
    10261 aatttcctaa gcacagctct agctacgaag aaatttctaa ttatctggaa ctgaatgtcg
    10321 actatttgcc gtccatggat atctttgacg aagcatggga aaaatattta gaaaataatc
    10381 atgacatata agagatagtt ttgtacttta tgatcaaaaa agttttttgt ccaatagcct
    10441 aattgaaaga attattttta tttcagctat ctagtcgcat ttttttgata tttttcttat
    10501 aataaagaaa gaatgaaaaa aagagggatg gattgataga gattgacaaa aaaacaacgc
    10561 tatattaatc gcttctggct tgtattcaaa tacttacttg tttctgtatt ccttgcgttc
    10621 atgttgcggg gatttttatt catcccggta ccagtggaag gcaattcaat ggaaaacgtg
    10681 ttgaagcaag gtgatatggt tgtgatggaa aaattttcgg agatcagacg atttgatatt
    10741 gttgtttttc aattagcaga tggcacgatt tatatcaaac gagtaatcgg tcttccagga
    10801 gaaaatgtca gctaccaaaa tgatcaattg aagatcaatg ggaaagtagt taaggaacct
    10861 tatctaacta aaaacctgaa atcagatcat gcaaatgcat cctatacgac ggactttaca
    10921 ctacaagagc taacgggaca atccaagctt ccggaagata attattttgt acttggagat
    10981 aatcgaagag tttcaaaaga tagtcgttcg tttggcacga tcaataaaac tgatattcta
    11041 ggtaaagcaa gatttgtcta ttatccactt gatgaaataa aatggatcca gtgagaaaca
    11101 ataaagcgtg cagtctgttg agactgcacg ctttattgtt taatccaaaa tcagtttact
    11161 accttagtac gtaaaatcaa acagctcgtt taaacacttt tctagcctct aaagttttga
    11221 taaatgaata aacaaccatt atttgaatag ggatttggat catattcctg atgaatctac
    11281 ctagaaaaat tactttgaaa tctgcaccaa acaaaacaga catcagccaa gaattgataa
    11341 aaaggctgac tgggatcata atcaataatt gggcagcgat gacggaaggc aggtttatct
    11401 ttttatgata taaaaataag ccattaataa atccaccaag gaaagccgtg ataagaaaag
    11461 cagcttgaaa cgttcctgaa ccaaatatcg tgaaatttaa agtgttcgac aacatttcaa
    11521 gcaaaccagc ccagatcggt ccatatagat aacccataag cgccatactt aaaaatccga
    11581 atgtgatttg aaaataagca ggaccaatcg ctatcttgta taagatgaca tcaagggcta
    11641 ataaaagagc agaggtcgac aacaatcttg cattcatttc gggcaatcta aaaacgtctt
    11701 ttgcaatcaa agtgttcaaa atattctctc ctttgtcagt taataataaa aggataccat
    11761 aaaatcacca atgtgtcatg acacattggt gatttacata cactggcatc taacaattaa
    11821 aaaaagggtg agaagactgc ttttataaac tgtaaaaaag agattgtacg aataaaaaaa
    11881 atagaggcgg agaatactta tcgtatcatc cgcctttgtt tttttgttat aaaaaacgtc
    11941 agattagtta tctgcttaca tcttcatcca tacgggttct aatgtatttc cgtctagatc
    12001 ttggacttct aatgaaaaca tttcttcttc aggcattccc atatctactt cgtggaaagt
    12061 accaccattt tcttttgcaa tttgagcaaa tttttttact tcatccgcat tttcaacact
    12121 aaacgaaatc aaagcaccac ttgttttttt attatcagct atttctttat cttttataaa
    12181 ttttttataa aaatcatgag agagaagcat tatccaaaaa gtgtcatccc ataccattga
    12241 accagcttct tcagtagaaa attcttcatt ttttttaaaa cccagctttt cataaaaagc
    12301 aacggaacga ttgacatcag caacaggaaa attaacaaaa accatactat tcatttgttt
    12361 cgtctccttt ctgttttgtt cgtctatcat ctcttattta ggaaacgctg tcaaaaattt
    12421 agattcatat atagaaacca aaataaaaat ttgcatcatc aaaaaagtga agatgtgata
    12481 gaatgaggac tggttcactt tcacttaact aatagatgga atttctttat aataaaaaag
    12541 tgaatttata ttgaaaaaga aaaaaacaag taaagccggg gaatattccg gtatgaaagg
    12601 aatggagaga acaatgacca tccaatggtt tccgggacat atggcaaaag cccgacgaga
    12661 agtttctgaa aaaatgaaat atgtggacat cgtatttgaa ttggtagacg cacgattacc
    12721 tttatcttct cgtaatccaa tgctagatca gatcattcag caaaaacctc gccttgtttt
    12781 attgaataaa gcggatctgg cagatccaca acagacaaag ctgtggcaag aatattttcg
    12841 taaacaaggc catcttgcat tagctatcac ggcacaagaa agtaaaggaa ttaaaggtct
    12901 agtacctgcg gctaaagaag cattgaaaga aaaacgggca agagatgctg ctcgggggat
    12961 caagcctcgc gctattcggg caatggtctt aggtattcca aatgttggga aatctacgtt
    13021 gatgaatcga ctagtaggca agaaaattgc acagacagga aataagcctg gcgtaacaaa
    13081 aggtcagcag tggcttcgtt taggtaatga gttggaattg ttagacacac cagggatttt
    13141 atggcccaaa tttgaagatg aagaaatcgg caaaaaacta gcgctgacag gcgcgatcaa
    13201 agaccagctt gtccatttag atgatttagc gatttacgga ttagactttt ttgcccgata
    13261 ttattcagga agaatcagcg atcgttatgg tttatcaatc gagcaagaag aaaaattggc
    13321 accggaatta ctgatggata ttacagcaaa acgtgggtat aaagaagact atgatcgtgg
    13381 cagcgaatta gtcgtgcatg atattcgtca aggaaaactt ggacgataca cactggatcg
    13441 ttgcgaagaa atggagcaaa aaagtgatga ctgaatccat ccaaagtatc aaagaacgat
    13501 tgaaaagcat acagagaata gatgatccta cactagaatc attaagaaat gatccacgca
    13561 aaggcgtgca gcaagcactg gcttcttttg aacgacgatt gaaaaaggaa gcattaatta
    13621 aggaaaaata tgaagaaatg ttttgctttg aaaagcaagg gtacgcacaa ggtcaccaat
    13681 ggattgccgg cattgatgaa gtaggaagag gaccattagc tggacctgtc gtggcagctg
    13741 cagttatctt gccgaaaaat gagatcatat tagggctgaa tgactccaaa caattatcag
    13801 agaaaaagcg agaacttctt tatcaacaag ttcaagaaaa agccctggca atcgggatcg
    13861 ggatcgttga tcagcagaca atcgatgatg tgaatattta tgaagcaagt aaattagcga
    13921 tgatccaagc agtagaaaaa ttgtccattg taccaagcta tttgttgatc gatgcgatga
    13981 ccttggattt gcctattcag caggaaaaaa taatcaaagg ggatgcacga tctgtgtcga
    14041 ttgctgctgc aagtatcatt gctaaagtat atcgagacca attgatgaaa gaatatgacg
    14101 tgctttatcc tggatatggt ttctcgcata atgcaggtta tggaacaaag gaacacttag
    14161 aaggacttga acgacaagga attacgccaa tccatcgtaa aagttttgca cctgtaaaaa
    14221 aatatttaca ataaaaaaca gccgctttta cgtatcttat tagaaagaga gacgaaaggc
    14281 ggttttatga tgtatcaaat agaagaaaat ttattgaaac ttgcttatac aaaaggaatc
    14341 agtagtcaag gaaaatgggc acttgctaac tggatgatgc agtatcctta taaagaaatc
    14401 ggatttgaag acatcattaa gataggtaaa ataaccaaac accgggagct gtttcgcaaa
    14461 agttggcagg aaattcacgc taactggggc aaaatccaat cgaaacagtc gtttatcact
    14521 tgctttgatc ccaaatatcc ccctcagctg cttcacttga cctatccgcc aattgtactg
    14581 ttttataatg gtgatctatc tttactttct tgtaacatgt taagcgttgt tggtggaaga
    14641 cacacttctg ctttagccaa aaaaacagtt tattatttac taaaaccagt gatagaagct
    14701 ggatatgtga tcgtcagtgg ctgcgcgaaa gggatagata catacggtca ccaagcagca
    14761 atctctcatg gtggacggac aatcgcagtc attggaacag ggatagagca agtctatcct
    14821 gctgaaaatc gtgacttgca agaggtgatt gcaaatgatc atcttctttt gagtgaatat
    14881 cctcctcaat ccggcccgaa aaagcattgt tttccaatga gaaatcggat tatcgcagct
    14941 ttgtctaaag gaacatgtgt ctttgaagcc aagaaaaaaa gcggctctct gataacagca
    15001 cagcaagcat tggatcttgg aagaacagtt ttttctgttc caggaaatat tttaacagaa
    15061 cattctaccg gatgtcatca tttgatccaa gacggtgcag tttgcacggt ttcgggtgaa
    15121 gatatcttgg cagaatttaa agaataacac catacttttc atttgctttt cttgacaaac
    15181 gttttctgaa tggatatgat tagctacgat ttgtatgaat cctcttttaa tagtaggaac
    15241 acgacaatgg aaaggagtcc attcagtaat ggcgtataaa tatttagtga ttgtagaatc
    15301 acctgcaaaa gccaagacaa ttgagaaata tcttggacga aactataaag taatggccag
    15361 tgttggccat ataagagatt taccgaaaag taaaatgggt atcgattttg aaaacaatta
    15421 tgaaccccat tatatttcta tacgcggaaa aggcgatgtc atcaaaagcc tgaaagccgc
    15481 agcaaaaaaa gctcaaaaag tttacttggc aagtgacccg gatagagaag gagaagcgat
    15541 tgcttggcat ttagcgtacc tacttgggtt ggatctgaaa gaaaaaaatc gggtggtctt
    15601 caatgaaatc acaaaagacg cagtcaaagc agcttttaag gaaccaagaa cgatcgatgt
    15661 agatttagta gatgcacagc aagctcgtcg taccttagac agaatcgttg gttattcgat
    15721 cagtcctatt ctctggcgta aggtcaagaa agggttaagt gcaggacgtg tccaatctgt
    15781 cgctttaaaa attattattg accgtgaaaa agagatccga gaatttgttc cagaagaata
    15841 ttggagcatc gacggtaatt ttaaaaaagc tcgcaagaaa ttcaaagcaa atttctgggg
    15901 aatcgacggt aagaaaaaga aattaccaga tgcacaaagt gtaaaagaag tcactgctag
    15961 aattgatgga acagacttcg aagttaaaaa agtagaaaag aaagagcgca aaagaaaccc
    16021 tgcgaatcct ttcactacaa gtagcctgca acaagaagct gcccgtaagt taaactttcg
    16081 aacaagaaaa accatgatgg tagcacagca attatatgaa ggaatcactt tgggtaaaca
    16141 aggaacagtc ggcttgatta catacatgcg tactgattct acccggattg ccgatacagc
    16201 aaaagcagag gccgcagcat ttattgaaga tacgtatgga aaagaatttt ctagacatga
    16261 tcaccgaaag atcaaaaatg ctcaaggcgc acaagatgcc catgaagctg tgcgtccaac
    16321 gagtgtgctt cgtacaccag atgaactcaa acaatattta gataaagatc aattgaaact
    16381 ttatacattg atctggtcac gttttgtggc tagtcagatg acaccagcta ttttagatac
    16441 aatgaaagtg acattagaac aaaatggtgt catcttcatt gcgaacggtt caaaaatcaa
    16501 attcaaagga ttcatgcaag tgtatgtgga aggtagagat gacggcaaag aagagaaaga
    16561 aaatattctt ccagaacttg aagaaggcga tgtagtccaa tctgttgata ttgaaccaaa
    16621 acaacatttt actcaaccac cagcacgttt tagtgaggct acgttgattc gtgcactaga
    16681 agaaaacggg gtagggcgac cttccactta tgcaccaaca cttgatacga tccaaagacg
    16741 ctactatgta aaattgacac aaaaaagatt tgagccgact gaactaggag aaattgtcaa
    16801 ttcattaatc tgtgaattct tccctcaaat cgtcgatatc catttcacag cagaaatgga
    16861 aggagatttg gataaaattg aagaaggcac ggaagcgtgg gtcaaagtgg ttgatcgttt
    16921 ctataaaccg tttgaaaaag aactgacgaa tgcggaagaa aaaatcgaaa aaattcaaat
    16981 caaagatgaa ccagcaggat tcgattgtga tgtctgtgga catccgatgg tgataaaatt
    17041 agggaaatat gggaagtttt atgcttgcag taattttcct gattgtcgga atacgaaacc
    17101 aatcgtaaaa gaaatcggtg tgacttgccc agtatgtcat gaaggacaag tcattgaacg
    17161 aaaatctaag aaaaacagac tattctatgg ttgttcccgt ttcccagaat gtgaattcac
    17221 ttcttgggat aaaccagtgg gcagaaattg tccaaaatgt gacggatatc tggtcgagaa
    17281 gaaagtcaaa ggcggtaaac aagtagtctg tgtaaatgga gattacgaag aagacatcca
    17341 aaaataatag aaaagaggtt ctttcattga aaatggtaaa tgttgttgga gcagggcttg
    17401 ccggaagtga agctgcttgg cagatcgcac aagcaggagt ccctgtccgt ttatatgaaa
    17461 tgcgtccagt caaatcgact gaggcgcatc acacaagtaa ttttgctgaa ctagtatgtt
    17521 ccaattcatt aagaggaaac agtttggcta atgcagtagg gttattgaaa gaagaaatgc
    17581 gccgattgaa ttcggtagta atcacttcgg cagatgaaac agcagtgcca gctggtggtg
    17641 ccttagctgt tgaccgcgat tctttttctg aaacaattac ccgaaaagtc aaagagcatc
    17701 cattagtaac agtgataaac gaagaaatta ctgaaatacc ggaaggaatc accgtagtag
    17761 caacaggacc tttgacttct catccattag cagaaagcat caaagcattc aatggttcag
    17821 atggttttta tttctatgat gcagctgcac caatcgtaga caaagctacg atcgatatgg
    17881 ataaagtata tttgaagtcc cgctatgaca aaggagaagc agcttacttg aactgtccga
    17941 tgacagaaga ggaattcaaa gctttccatg aggcactaat caatgccgaa gtggcagaac
    18001 ttaaatcttt tgagaaagaa aaatattttg aaggatgtat gccaattgaa gtaatggcac
    18061 agcgaggatt caaaacgatg cttttcggac ctatgaaacc agttggattg gaagatccta
    18121 aaactggcaa acgtccttat gctgttatcc agctgcgtca agacaatgct gcagcttctt
    18181 tatacaacat cgtgggcttt cagactcact tgaaatgggg cgagcaaaaa cgtgttttcc
    18241 ggatgattcc tgggttagaa aatgctgaat tcgttcgcta tggtgtgatg caccgaaaca
    18301 gctttatgaa ttcgccagaa ttgctgaaac cgacttacca atcaacgaaa cgcgaagatc
    18361 tgttctttgc aggtcagatg actggtgtag aaggctatgt tgaaagtgca gcgagcggac
    18421 tacttgctgg aatcaacgcc gcacgcttag caaaagaaat ggaaccagtc gagtttccac
    18481 aagaaacagc tattggaagt atggcttact acattactca tgcggaagga aaacatttcc
    18541 aaccgatgaa tgccaactat ggtctgtttc ctgaactgcc agagcgcatt cgagacaaaa
    18601 aagcaagata tgaagcaatt gcacaacgag ctttaaaagc taatgaggca ataaaaacag
    18661 aattagaaaa aactgcaatc ccaaatgagt aaggaaactc catgaagagg gtgagacgga
    18721 tagtctcgct ctttttcttg cgtttttctt aagaatttct caaacaaaat gtgaggaatt
    18781 gcgccaattg tgaacaaact tagaaatatt ggttaaattc tgacaattta tttgtctttt
    18841 tataaacgct gtgctaaatt attctctggg acaaaaggaa ggtgagaaaa tgacagaagc
    18901 cacgtgggaa caaaaatttt tgcgttattt gattgtcgaa agaggatatt cagaaaaaac
    18961 gaaagaagca tatgaagaag acattctcca tttcaaacat tttttggagg aatctggaga
    19021 cgctgatctg ctgaaggcgg aatattttac ggttcgtgtc tatttgagtg cattagctga
    19081 tcatcgtcct agctatagca gaaattctat cagtcgcaag atagccagcc tacgttcgtt
    19141 ttatcagtat cttctcaaag aagaagtgat aaaagaaaat ccattttctt acgttcatct
    19201 caaaaagaaa aatttgagac tgccacgttt tttttatgaa aatgaaatgc aagcactttt
    19261 tgacagcgta gcaggagata gtcctttgga tttgaggaac cgcgctttac tggaagtcct
    19321 ttacgggtca ggcatccgtt taagtgagtg tagcagtttg tcggtatcag acattgattt
    19381 tgattcagag gtcatgttga tccatggtaa agggaacaaa gagagatatg ctcctctagg
    19441 ttcttttgct caagatgctc tgcaagaata tttcagggaa ggccgccagg tattgatgac
    19501 aaaatatcac gaagagcatg atatcgtttt tgtgaatcat catggaaaac caatcacgcc
    19561 tactggaatt gaatatgtct tgaatcaaat gatcaaaaaa agcagtttgg atagtgatat
    19621 ccatcctcac atgctgcgcc atacatttgc tactcattta ttaaataatg gggcagattt
    19681 gcggacagtc caagaattgc taggacatgc caatttatcg actacccaga tctatgccca
    19741 tgtaacaaaa gaaagtttac aaaaaaacta tcgttctttc catccaagag cgtaaaaata
    19801 aggaaaagaa caaatttagg aggaaacaaa catggtcgaa tcaccatttc attcaaccac
    19861 tatctgtgcc gtcgaaaaag acgggaaatt tgctatggct ggagatggcc aagtaacaat
    19921 gggtgaatct gttgtcatga aaggcactgc aaagaaagta cgacgtatct acaatggaga
    19981 agttgttgta ggtttcgccg gaagtgttgc agatgcgttt actttagaag aaaaatttga
    20041 aggcaaacta aacgaatata acgggaatct acaacgcgca gctgtcgaat tagctcaaga
    20101 atggcggaca cagcaatcta tgcaaaaact tgaagcgatg ctgatcgtaa tgaatgataa
    20161 agaaatgctt cttgtttcag gtacaggaga agttatcaca ccagatgatg gtatcttagc
    20221 tatcggatca ggagggaatt ttgcgttggc tgcagcccga gcaatgaaaa actatgctca
    20281 aaacgacatg agtgcgaaag aaattgcaga aaatgcgtta aatattgccg cagatatctg
    20341 tatctttact aatcataata ttatcgtaga agaactgtaa gaggtgagta gatatgtatg
    20401 aattaaaaca aacaccaaga caaatcgtaa gtgaattaga tgaatatatc gtaggacagc
    20461 acgcagcaaa aaaatctgtt gccgttgctt tgcgtaaccg ctatcgccgt ttgcagttag
    20521 atgaaaaaat gcaacaagat attacaccaa aaaatatgct gatgatcgga cctacagggg
    20581 tcggtaaaac agaaatcgca cgacgtttgg caaaaatcgt caatgcacca tttgtcaaag
    20641 tcgaagcaac aaaatttact gaagtcggtt atgtcggaag agatgtagaa tcaatggttc
    20701 gggatttagt agaaaatgcg atccaaatcg tagaaaaaga gcagtacagc cgtgtataca
    20761 caagtgctaa aaaaaatgca gaaaaacgtt tggtaaaaat cttggtacct ggtatcaaaa
    20821 aagaacagaa acaatccact aatcaatttg aacaaatgat gaatcttttt aatgctagtc
    20881 agcaaaatac cgctgaaccg caagaagaag tgacagaaga tattcgaatc aatcgccgta
    20941 cagtcttgga acaactacaa aagggtttac tggatagtcg tgaagtaacc attgaagttg
    21001 aagaccaaaa gaaacctgca ccaatgatga acaatggatt ggagcaaatg gggatcgacc
    21061 taaatgaaac tctaggtgct ttgacaccga agaaaaaagt ggaacggacg gtaacagtca
    21121 aagaagcttt agagttattg atcaaggaag aatcagcaaa attagtcaag gatgctgata
    21181 tccatagtga ggcaatcaaa ttagcggaag aaagtggaat tatttttatc gatgaaatcg
    21241 ataagatcac ttctaaatca cagcaatctg gagaagtatc cagagaaggc gtccaaagag
    21301 acatattgcc aattgtcgaa ggttcacaag taaacacaaa atacgggatc atccaaacag
    21361 atcacgtcct attcattgct tcaggagctt tccatttgtc taaaccaagt gatctaattc
    21421 cagaacttca aggacgcttc ccgatacggg tagaattaga tgatttgaca gctgaagatt
    21481 ttgtgaagat tctgaccgaa ccaaataatg ctttagtaaa acaatacgtc gcgttgatcg
    21541 gtacagaaaa tgtgtcagtt acgtttacaa aagaagcaat tgaacggatc gctcaaatcg
    21601 ctttcgatgt caaccgtgac acagataata tcggcgcacg cagactgcat accattttgg
    21661 aaaaactact agaagatcta ttatttgaag caccagatat gcaaatggga gaaataacga
    21721 tcacagaagc atatgtggat gacaaacttg gctcaattgc ggcaaatgaa gatttaagtc
    21781 gctatatttt ataaaaaatc ggaggacgac aatgacagac ttgctaacga aaacccgcaa
    21841 gataaacaca cttttgcagc aaaagaatac ttttgacatg caagcagatt tgccttacga
    21901 taaaatggct gtcacactag gtggtattct tgacagcaac acctatatca taggaagtga
    21961 cggagtatta ctaggttata atgaacgtca cgatgtgaac aatgaacgaa tcaaaacaat
    22021 gtttgttgag aaacaatttc ctgaatctta tactgataca gtggcacagt tgatgaaaac
    22081 tgaagctaat atcccaatta ctagtgatat tactgcattt ccggtagagt caagagagaa
    22141 atatccattt ggtctgacga ctgtcgttcc gatctttgga gctggcgaac gtttgggtac
    22201 gattatctta tctcgtatgg aacaatcgtt tgcagatgac gatctagtct tagcagaata
    22261 tggagctacg gttgtcggta tgcagatcct ttatcagaag tctcgaagca tcgaagcaga
    22321 tgtacggagt gcaacggctg tccaaatggc aatcaacaca ttatcttata gcgaattgaa
    22381 ggctgtacaa gctatctttg aagcattgga tggagatgaa ggtcgtctga ctgcttctaa
    22441 tattgccgat agtatcggca tcactcgttc tgtgatcgtc aatgctttac gaaaattgga
    22501 atcagctgga atcattgaat ctcgttcgtt aggcatgaag ggaacttatt taaaagtatt
    22561 gaattcccgt tttaaagacg agttagcaaa acacagttat tagaagtaag gggactgggg
    22621 cagatagaaa tgtttgttcc agccttccca ttttaaaaag taagtcgaag ttggaggaat
    22681 ggcaatgact gttattattc aaaatgacca gctgatcgcc gaaatcgctg aacacggtgc
    22741 ggaattgatc agtctgaaat caaaagaaca tggtttagag tatatctggc aaggtgatcc
    22801 agcgtattgg ggcagacatg caccggtttt attccctttt gttggacgtt taaaagatga
    22861 tcagtatgtt tatcaaggga aaacatatca aatggggcaa cacggatttg ctcgggatat
    22921 ggattttgaa gtcattgaac atggacaaga aatggctagt ttcatcttaa ggagtacgcc
    22981 ggagacatta gaaaagtatc catttgattt tgaattagtg atcagctatg aattaggagg
    23041 agacggcatc acggttcatt atcaagtggc aaataccggt gaaaaagaaa tgttcttttc
    23101 tatcggtgga catcctgctt tcaatgtacc tcttgaagaa ggattgtcat ttgaagattt
    23161 ctatctggca ttttctccat tgaaatcaag attacgccta ccattgaaag gcccattcat
    23221 cgatatggat caaaagacgt taggtcagac aaatacgaat ctggctttga atcgtgaact
    23281 ttttgagcaa gatgccttgg tttttgaaac aaggggtcta aatgcgtacg ccattcgaag
    23341 tgaaaaatct tcccatagcg taactttaag ctataaagat atgccttatg tagggatctg
    23401 gtctccttat ccaaaagaag cgcctttcgt ttgtatcgaa ccgtggtgcg gaatcgctga
    23461 cacggtgaac agtagtggaa acttgatgga aaaagtaggg atcaatcaac taggtgccca
    23521 tgaaatcttc aagacaaaat attccatcac agtgaagtaa gaggttgtga ccgagaagtt
    23581 agcagggaac accgtttacc aaggtgtgta aaaaagctat cctgtaagta ataagggagt
    23641 gtgacatcga ttt
//
LOCUS       DACEPW010000026        22684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEPW010000026 DACEPW010000000
VERSION     DACEPW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.026"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..231
                     /locus_tag="IW819_000801"
     CDS             <1..231
                     /locus_tag="IW819_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1243924.1"
                     /translation="GIRLAAVYVIAWATLASYIGAGGLGDLIFSGLNNYQPDLILAGT
                     IPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            228..1145
                     /locus_tag="IW819_000802"
     CDS             228..1145
                     /locus_tag="IW819_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1243925.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            1142..1813
                     /locus_tag="IW819_000803"
     CDS             1142..1813
                     /locus_tag="IW819_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1243926.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(2354..2842)
                     /locus_tag="IW819_000804"
     CDS             complement(2354..2842)
                     /locus_tag="IW819_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ1243927.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(3043..3309)
                     /locus_tag="IW819_000805"
     CDS             complement(3043..3309)
                     /locus_tag="IW819_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1243928.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(3309..3572)
                     /locus_tag="IW819_000806"
     CDS             complement(3309..3572)
                     /locus_tag="IW819_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1243929.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(3565..3837)
                     /locus_tag="IW819_000807"
     CDS             complement(3565..3837)
                     /locus_tag="IW819_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243930.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            4048..4233
                     /locus_tag="IW819_000808"
     CDS             4048..4233
                     /locus_tag="IW819_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1243931.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(4375..6198)
                     /locus_tag="IW819_000809"
     CDS             complement(4375..6198)
                     /locus_tag="IW819_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1243932.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(6796..9501)
                     /locus_tag="IW819_000810"
     CDS             complement(6796..9501)
                     /locus_tag="IW819_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1243933.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(9807..10754)
                     /locus_tag="IW819_000811"
     CDS             complement(9807..10754)
                     /locus_tag="IW819_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1243934.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            10944..11213
                     /gene="rpsN"
                     /locus_tag="IW819_000812"
     CDS             10944..11213
                     /gene="rpsN"
                     /locus_tag="IW819_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1243935.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(11389..11751)
                     /locus_tag="IW819_000813"
     CDS             complement(11389..11751)
                     /locus_tag="IW819_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1243936.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(11762..12883)
                     /locus_tag="IW819_000814"
     CDS             complement(11762..12883)
                     /locus_tag="IW819_000814"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1243937.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(12899..13249)
                     /locus_tag="IW819_000815"
     CDS             complement(12899..13249)
                     /locus_tag="IW819_000815"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1243938.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(13379..14890)
                     /locus_tag="IW819_000816"
     CDS             complement(13379..14890)
                     /locus_tag="IW819_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1243939.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(15212..16564)
                     /locus_tag="IW819_000817"
     CDS             complement(15212..16564)
                     /locus_tag="IW819_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1243940.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(16717..17793)
                     /locus_tag="IW819_000818"
     CDS             complement(16717..17793)
                     /locus_tag="IW819_000818"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1243941.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            17989..19734
                     /locus_tag="IW819_000819"
     CDS             17989..19734
                     /locus_tag="IW819_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1243942.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEEPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(20014..20451)
                     /locus_tag="IW819_000820"
     CDS             complement(20014..20451)
                     /locus_tag="IW819_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1243943.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(20435..21808)
                     /locus_tag="IW819_000821"
     CDS             complement(20435..21808)
                     /locus_tag="IW819_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243944.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(22130..22618)
                     /locus_tag="IW819_000822"
     CDS             complement(22130..22618)
                     /locus_tag="IW819_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1243945.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
BASE COUNT         6456 a         4831 c         4182 g         7215 t
ORIGIN      
        1 ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta cacttgcctc ttatatcggc
       61 gctggtggtt taggcgattt gatttttagt ggactgaata attaccaacc agatctcatt
      121 ttggcaggta cgatccctgt aacaatttta gcactattgg ctgattggct tctaggtctc
      181 ttggaacacc ggctgacacc gatcgcactt agagaggaga atgaagaatg aaacgtatac
      241 taaaaatgac catggttctt ttctctcttc ttatacttgc gagttgttcc ttccctggtc
      301 ttgccagcaa tacagatgaa gacaccattt cgatcacagg tggaatcact tcagaagcac
      361 agatcttagc aagtctagtt gccggaatgg ttgaacatta tacagataaa aataccgcaa
      421 tcatcaacaa tctggctact acaactatca accatcaagc gatgatgaac ggagatgctt
      481 caatttcagc ggctcgctac acaggaacag atttaacgac tacattgaac cttcctcctg
      541 aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga atttgagaaa agatacggtc
      601 aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt ctttcttgtt cgaaaagaca
      661 ccgcacaaaa ataccatcta tctaaagtca gcgatctgaa aaatgtagcg gatgaattag
      721 tagctggtgt agatacttct tggatcaatc gtaaaggaga cggttatgac ggcttccaag
      781 aaacttatgg cttctccttc aattcgatat tacctatgca gattggactt gtttatgacg
      841 cagtcgaagc aggaaaaatg gatattgttc ttggctactc tacggatgga cggatcgcca
      901 gttatgatct agtcatgttg gaagatgatc gtcaattctt cccaccatat gatgctgctc
      961 cagttgtaga caacaagata ttagctgata cgcctcattt aaaagaagcc ttaaataagt
     1021 taggtcatac catcagtacg gaaaaaatgc aggaattgaa ctacgaagca gataataatc
     1081 tagtggaacc atcagtagta gcacaacgtt tcttacaaga aaaccactat tttgaaggga
     1141 agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt ttctattatt tccaggaaaa
     1201 tggctcctat atctttgcac aatttatccg tcacttcctg atttctatct atggtgtcct
     1261 ctttgcagca gttgtcggga ttccggttgg gatcatgatt tctaggagac gtaaattagc
     1321 aaactgggtg attcgtttgg cgaatatcat ccagacgatc ccttctctag ccatgatctc
     1381 cattttaatc attggattag gcctgggagt caatgtcgtg attgttactg tatttttgta
     1441 ttccctctta ccgatcatca agaatacgta taccgggatg atccaagtag acaaaaacat
     1501 ccttgatgtc ggcaaaggca tggggatgac tgctagacaa cgactattca tggtagagtt
     1561 accactctct gtttctgtta tcatggcagg cataagaaac gcgttagttg ttgccatcgg
     1621 gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt ggagatatca ttatccgagg
     1681 aaccaatgca acagagggca catcgattat tttagcaggt gcattgccaa ctgcattaat
     1741 ggcgatcatc acggactggc ttttaggtat tttggaaaga cggctagatc cagccagccg
     1801 tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg cgaccatttg ttggtcgcaa
     1861 aggctttttt agttctgtca tttttaaggc aataatccac tctgattctt ccctccttat
     1921 gggcaaagag ttgcttttct tagctattta tccattccgt atactccaac ttcatactgc
     1981 caacgggttc atatctgcat ccaccgacta agtttttttc ttatccgcta aggcattttg
     2041 ccaatccatt ttcccatcac tcgtcatgga attcatacca catgatctct aaatactcgt
     2101 ctgcttgttc tttgaattgg gcatcaattc ttacatcctt tggcgtggct ttcatttaag
     2161 ccttttccat cagtcctcga ttgttcgatg ccttcagctg catcatcaaa actggtgact
     2221 gccgtatcgg taacttcgat catcttttat acaatcggga acttgccatc ctcatccttg
     2281 gctaaaaaca tatctagcca ttccagcttc tcgccatggg aggtttgcgg ttcctaaaac
     2341 cggtacacga ttcttatgga ctgcatccgt cgcaactaga cttggcgata cgatgatcct
     2401 tcaccagaag agcacccccg taaaccaatt gatcgatata ttgccaataa gaaaagacat
     2461 tggcgtaaaa gatatcgatc cctcgtgatg cattgccgtt gatactgcta ttcatggccg
     2521 aaagcgccac gactttaact tcttccgttt gagtgacatt catgttcggc aatgtcgctt
     2581 tggatacccg ctctgctaaa ggaacgacac tggtattgta tttcgtatct gtatcttttc
     2641 caaaagacca tgccaacgaa tcctctggga accaataaga cgactccggt tgattgtcca
     2701 ttgctttttt caacatactt ttggctgttt ctcgataaac gacatttcca attgtcttcg
     2761 cctcactttg gcgataatcg atggtcgttg ccactgccaa cactaacaac gatgctccaa
     2821 ttttactgat tttccttgtc acacttcttc ctccttgttt tacataaacc cgtcaacatt
     2881 ccattcctaa actcaaacaa tgacccttca aaggtggaga tataggaaca aattaaagaa
     2941 atcatgcaat aaatccaaga aggttggttt tagaacaaaa aaagagtctc ctttttgggg
     3001 agactctttg ttaactatct aaaaaggttg gctcttctca cttcactcat aatgtgtcca
     3061 ggcagaataa atttctacaa ctttattttc ctcatcaact ttgtaaacca ctcgatgctg
     3121 tctattcagc ctgcgtgagt atctgccttc atgtgtaggt ctcagctttt caaaagactg
     3181 cgtgggcata tatggatctt ccttcaatgt ctgaatcact tcttcaaatt gtttctttaa
     3241 attcgtttgc ttgatttttc ttaaatccac tttcgcagag tttttgattg cgactgtata
     3301 gttactcatc aaagagcttc ccaatcaata tcatcgatat tcgtaaagct actgtcgtct
     3361 ttttcgcggt ctcgtacttt atctaaagta cctgtttgct caagaaaaag ggtttcttga
     3421 atcgctctcc agtcttccag cccaatcaat acagcattgt ttccactacg atcactaata
     3481 atctcgattt cagtatgatt ttcatttacc tctttcaata actgataaaa atttttacga
     3541 gcagtgctag gattcatcgt tgtattagcc attgtctctc ctcctttttc tgatataatt
     3601 atagcgtact tgattaagta cgtcaaaaaa atacaataaa gcacttaaat tcagcaaact
     3661 tttcctcttt tttacataga gaaaaagacc cctttttagg gaggatcttt gtccacgctc
     3721 acttcttact cctttacgac tagcggtagc caaacgtaaa gtaattggta acaacgtatc
     3781 atctttagca aaggtcgaat ccaaagcctg tgagtctatg caacaacacc aatacacgaa
     3841 ggttcctcgt tgcagtgtga cttgattgat atcttgaata acctgaggat acgcacgaaa
     3901 gatttgtaac ggttattcaa aactgatttt ggccgtcaag tcttctactg cctcaaaaat
     3961 gattgcctct ttcgtctcct gtcattcatg ttcactatcg atttgatact tgcttagacc
     4021 gttgggtttg tgaatcacca attaaaaatg gcactgagtt atggtaacaa aaaatggtca
     4081 cttgacgagg ctgagattcc tacttatgaa gacacatggc acttaagagt cgaggtaaag
     4141 gaagaagctg aaaatatagg aggctgtaca ttatttggca aaggattcgg aaattatgat
     4201 caagacatca aaatcaaaat gttttacagc taaataggaa gaataataac atttcatacg
     4261 ctgttcccat ctttaatggc ttatagaggg cacctttagc tgagaataag acaaaacaga
     4321 ccctctagct tcaattaaga gctagagggt ctgtttttga ttttaaaact tggtttattt
     4381 cttcaaatgg tagctatagg tgctgacaac gatattttca cgccaagaca atcgcaatct
     4441 taaccgcaaa cttgtttggt tatggagaat attctgccag cgtctgtttg gtacaaattg
     4501 aggaatcaac acagtagttg tatagttttg tttcgctgca tttttactta cgatatctac
     4561 ataacggata attggatttt caatcgagcg ataagaagag tggacgattg aaaaacggac
     4621 atctgggaaa tgctttttaa attctgcttc gatttctttt tctttttcga catcttcgtc
     4681 tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa cgtgcgtaat tcaacgcacc
     4741 gatatttacc cgtgtcacat tcccgactaa tacaagaacg gtattcccgt cataatcatg
     4801 tagttttgca tcattttcta aacgaagctg ttctgctact tttttataat gatcatggat
     4861 cttataaaaa atgaacataa cgattggcat aatgatgaag aaaggccaaa tgtcccctaa
     4921 ccgatagaca aacaaaatcg caatgattgc atatgagatg aacgcacccg taatattggc
     4981 aatcgatttg cttaaccatc tttttccttc ttttttccat ttgacgacca tccctgtttg
     5041 agataaagca aatggaatga acacgccgat agagtataac ggaatcaagc gttctgttga
     5101 gccatggaaa ataaacagca atacgatcga acctagcgct agtgtgataa tcccattgga
     5161 atagcctagt cgatccccac gatcttgata catgtgaggc atgaatttat cttttgccag
     5221 attgtatgca agaactggaa aagcagaaaa accggtgtta gcagcaactg ctaaaatcag
     5281 cgctgtagca aattgaagga cgtaatacaa aataccatgt ccaaagacag ctttaccaat
     5341 ttgagacaaa acagtgactt ctttttcagg aacgatccca taccaatagt tgataaatgt
     5401 gatccccacg aagaagaaac ctaagatcaa tgccatcatc gtcaatgtag cagcggcatt
     5461 ttttgctctt ggcttcttaa agaatggtac agcattacta atcgcctcca ccccagttaa
     5521 agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg atagaaacac ccggcacagc
     5581 cgctcctgga agtgcagtag cttgcaatgg cacagcacct gtcacgatat tatataaacc
     5641 aacaacgatc agtaacgaga tcacaaggat aaatgtgtac accggaaata ataggaacga
     5701 tgcagattcc ctcagcccac gaagattcag catcatcagt agcaaaacga ttgtcacaga
     5761 aatagcgact tgatgaccgt agagtgctgg caccgctgat gtgatcgctt cagcacctgc
     5821 tgagacagat actgctactg tcaacatgta gtcgatcaat aaagagccac cggaaatcaa
     5881 gccagcattc tttccaagat tctcactgct gacgacatat gctcccccgc catgaggata
     5941 agcatggatg atttgacgat atgatagcgt taatgaaatc aataaaataa taacaaaagc
     6001 ggcaatgggt agcgaatacc agatggctgc agcagacaaa gcgactaaaa cgacaacgat
     6061 ttgttccgta ccatatgcaa tagatgataa cgcatcagac gacaataatg ccaaagcagc
     6121 aaatcgggtt aatttgtgct catcattttc agcagatttt aaaggcttac ctactaacaa
     6181 cctttttaag taatccaatt tctaaactcc tttacacaca attttacttc aagttttata
     6241 gtcgaaaatt tatagtaaac aagatgttat agtacgctca ctgaccttaa aagtcaataa
     6301 actttaagaa aataattgga aaaaaacaga aaaatatcaa gcgctttttc aaacgcttta
     6361 atacagcaaa aatctaacat ttttttatgg gctttttctt tgaattctat aataaaagga
     6421 tagaacttga cttagaaaat caagttctat cctttttgat atcttaactt ttggaagaga
     6481 gatgtaaggc tgctttctac ccatcattct tactactcga agcatagcgc agttgtcaca
     6541 acctcttgat aaacggtgtt taaaagcttg catctgcggt aataagccca accaaattaa
     6601 aaacataaga agctgttttt atttggtcgt tcttattact tgatgcagag cagctttttt
     6661 acaacctctt ggtacacggt gttcaatcag ctgaccctcg gtattagctc aaaaaaacgg
     6721 caaaatatga ggaactattt ttccattttt ttctctaata ctcaggtcaa aacactgatt
     6781 tcacaacctc tttttttaat gaaaaatatg gctctcatgc gccgcttctt tgaatttcgt
     6841 cattttttct tcgatgaacg gatagattgc cgttcctacg attccaaaga caacaaacaa
     6901 accgatcatg atccagattg ctttcgtagc atttggccaa taaattcctc ctgcagattc
     6961 ccgtaacaag tttaccgcat gagtaaatgg caagaacgga ttgatcattt ggaagaattt
     7021 ccctgagact tggatcggat agttcccgcc tccgccagag atcgacagta ctaagatgat
     7081 aatagcgatc cctttaccga cattatcgaa taatgcagcc aagacataga cgatcatcat
     7141 gaacgctaag gcgatcaaga cagcaaatga cacgctgtag accggatttt ggacatctac
     7201 tcctagtaag aaaatattcc ctaatgtaac gatcaagctt tgcgcaatcg ccattgtcaa
     7261 aaatgtcagc atacgagcaa cgaattgttc tcgtttcgta aaattgtttc ggtctttctt
     7321 tcctaaatag tattctgtcg tagcaacact tgaaagaaga acagcgccga cccacagaca
     7381 tagtgcagta taaaacgggg tgctagctga accgttattt gcgattggat aaagcgcatt
     7441 ggtttgtagc tcaacaggtt gtgtaaagaa gtcactttct tttgttgcgt ctagtttcaa
     7501 taatttgatg acttcgccta gatcgacttc ttgttcgcct tttcggatcg cgtctgctgc
     7561 tttttggata ccagctttga tatttggcca gtcattgttg atcaagtccg cagcagcgtt
     7621 cacagctgtt tcgacttctg gcattttttc atttactgtt gtcaatgtac cggtgatttc
     7681 attttttagt gctggccaat cattttggat aaaatcagcg gccaatccta atttttgctg
     7741 aatgatcggt aattcattgt tgtataggtc tgccccttca ttgatgccat tgacgatggt
     7801 atccatattt ccattcagca ttgtattggc atcatggatt tcttgtccaa ttgcaggcat
     7861 ttctttttga tatttttcta aaattgccac tgcattcgtt acagttgctt tcgttgagct
     7921 gagaagcgaa tcaaagtcga tctggtccgc tttgctcagt gcgccttgtg cagtcccaat
     7981 cgtatcgatg acttggtcta gtagagaagt gacagtggaa ctgatttgac ctgtattgat
     8041 accagcaatg atgccattga tattattaac aacggcatca atttgatcta gcaattcatc
     8101 aatagccttc gtgtcttcat tttgaacagc tgtgttcaaa gcagataggc ggttttgcag
     8161 attgcttagc aagggtttta attgatttag ctgatcaata attggctgta atttcgtatt
     8221 tccagcactt gtttgcaatt tagtcaggaa ctcgaccagt ttatcgattg cattttgttg
     8281 ttgagatatt ccgtcaataa agccgttcaa tacattgctg atgtttttct tttcatcatc
     8341 agtcagctta ccatcttcaa tagctgttct tacagtatct attaacgaac ctgtcgttgt
     8401 tgccacttgt ccaattgact tcaatgtaac atctacactg tttgtgatgc ttggtatcgc
     8461 atctttcagc tgagaggcac ctgattttgt ttggtttgct agatcacttg cctgattttc
     8521 taaatttttc acatctggca agatatcttg tacttgttgg atgatcgtca agccttgttt
     8581 tgcttcatcg attccttggt tcattgtctc ttggatcgaa gcaaaatcgc cgtcgatctc
     8641 tgctagttgc ttaccggcat tttggatctc tgggattttt tcttgcaaat caaggatgat
     8701 ttttgcttga tctttcaact caggcatttt gtcattcaat gcaacgactt tttcacccat
     8761 ctcatctact ttagggatgt aatccacgaa ttcatttgct ttattcagtt tttccttgat
     8821 ttcaggtagt tgactttgta attcgaggac ttgttttgtg tagccgtcga tcgtgtccaa
     8881 attttcatct gttgataaaa tcatatcttt aactttattg atacttacta ggttcgtatc
     8941 gatgtcgtaa cctatctcat tgaatacttt caataaagtc gaactggctg ttttgataaa
     9001 ttcatttgtg atttgcgatt ggatcgaact tgctccttta tcggtgattt tcggtgcaat
     9061 cgcattgatt ttttcattta ccgtatattc gatttttggt ttcttgatat caccactagt
     9121 aaaactcagc agatcttctg agaaatcctt tggcaagtaa atacctgcgt aatactttcc
     9181 ggaacggact ccgtcttcta attgttcttt tgaatcaacg aattgccatc ctaattgttt
     9241 atttttatga agcgattcga tgacttgctc cccaatggcc acttctttac cttgaaactc
     9301 tgctggcttg tctgcactgt aaacagcaat tggcagctct ccagtatttc catatggatc
     9361 ccataatgct ttgatgttaa accacgcata tagcgaaggg atgatcatga tcgcgatgat
     9421 cagaagtgtt gcaatcgggt ttttgaaaat ccgtttccag tcaagcaaat ataatcgaag
     9481 cgtatttttg attgactgca tagttctccc ccttcttctt ttttcagctg aattacttta
     9541 ccatgtattt catacttttt tcaatcggct tttttcaaaa aagtcgaatt tttggacata
     9601 tcacggcaat tgtctcattg taatctctta catattgaga aataattaag gattacagcc
     9661 tttactttat catgaaataa aagggaaaga taattttatg ccaaagtttt tatctattat
     9721 acgtatacgc aaaaaagcta aaacattttt tgtctttgcc cctacaaaaa tgtttcagct
     9781 tttttcgttt tttattcctg tatttcttat ttttcgaatg acaggatgat tttcccagca
     9841 ggaggatgtc cttcaactag ctgatgtgct tggatgacat gcttaagtga agcagacaga
     9901 ttcattgaaa taaaaacatg aaaggctcct ttttggtagg ctttgattat gccagcaaag
     9961 gcttctgcat ctagatattc ctttcttgga acaaacgatt cataaaagcc ttcctttttt
    10021 tgccgcaaat ctagatctgg gaggtcattt aatgcaacat acctgccgcc tggtttcatg
    10081 atctggatgc cggtttctcc tttgatactt cctttcgtcg catcgattac aaggtctgcc
    10141 tgatcagcaa attgcagacc tggatttgtt ttatcataag cagcaaatgc tgaagctcct
    10201 aattttttta ccttttcctc atttttactg gatgcactgg ttaagattcg gattcccttt
    10261 tcatggagaa gctggatcag acttgacccg accgcacctg atgctcctag tatcattaca
    10321 gtatctgtcg gctggatttc cgtcaaatgg ttgattagat tataagccgt aattcctggc
    10381 gtgaccattc cggctgcttc ttcccaactc attttatctg gaatttttgc tacttttgct
    10441 gatggcaaca cgacttcttc tccgtaagtc ccgccgactg catggacagc tactcgatca
    10501 cctacatgaa cagttgtgac atctgaacca atctctgtca caataccagc gccatcatta
    10561 cctggtacat aagggaattt caatgttcgt atctctttca tttctcctaa acgcaaggca
    10621 acatcataag gattcacact gaatgctttt atttctacac gaacatgtcc agaaccgact
    10681 tctctgggat gagcatcgat ttcttcaaaa acatcttgtg cttctccgta accgttaatt
    10741 gcaaatcttc gcatggtcac catctccctt tttctttaag tttacgttta tgcctttatt
    10801 ttgacaaaaa aaatgtcttt tcgttttggc gcatgattca aaacaaaata gttgtctttt
    10861 ttcgcgtaac gtgttaaatt atttatctaa aagcgtaaag attacgattt acgctaataa
    10921 aaaataattg gaggaatttg ttgatggcta aaaaatcaaa gattgccaaa gcaaaaaaac
    10981 agcaagaaac gattgaacgc tatgctgctt tacgccagga attaaaagcg caaaaagatt
    11041 acgaaggctt gcgtaaactt cctttagatg ctcgtccaga acgattgaaa aatcgtgatt
    11101 taatcgatgg acgccctcgc ggatacatgc gtaaattcgg gatgtcccgt ataaaattcc
    11161 gtgaattagc acataaaggt ttgattccag gagtaaaaaa agccagctgg taatccaagt
    11221 ctggctgtaa tgtaaaggag gagggcgcat ggaccactcc ttggatcatc aaaaaagata
    11281 cgttcgcttc ttctagcaga aatgctggta ctagaagcag acgtatcttt ttttacgcac
    11341 agctggtcct tttttatatg taaaggctgc atggttactt cgctatattt atttagtaac
    11401 aggagtcagt gcttcgatac ccacactaat tttcaatgct cggtcaactt ctgccataat
    11461 gggtcgatcc aaatggcaga ccttttcttt caatcttgat ttatcgatcg ttctgatctg
    11521 ctccaacagg ataacggagt ctctttctat tcctgttcca gtagctttga tcccaatgtg
    11581 agtcggaagc ttaggtttag ccatttttgc cgttatggcc gcaacaatga tcgtcgggct
    11641 aaaatggtta cctagattat tttgaataac caaaactggg cgtgttccgc cttgctccga
    11701 tcccacgact ggagaaagat cagcaaaata aatatctcct ctttttacca taggatcctc
    11761 cttattcttg atattctctt ggtatccgct gtcctaatcc gcaagccact tcatagtgga
    11821 tcgtacctaa ttgatctgct atatcctgca tagtgatttc tttgtctgca tttttgccga
    11881 taagcgtcac ttttgttccg acaggaaatt cttgaggcaa tcggatcatc agttgatcca
    11941 tacatacacg cccaatcgtt tcacagtagt tcccttctac caatagagaa aaaccctgca
    12001 tctttcgtgg ccagccatct gcataaccga tcggtatcgt tccgatccac tctgcttcag
    12061 gagtgatata tgtttctcca tagccgattc cttcacctgc tgggagtttt ttgacttgaa
    12121 tcaattcaga aaccagctca agtgctggct gcaatggata aacttccggt agtgcgtgcc
    12181 cagagggatt aagtccgtac attgctactc cataacgaat catattgccg attgtttcat
    12241 catgccataa agccgttgca ctattgctga catgtacata tcgaggaagt tcgggaagtt
    12301 ttttcagcac ttctataaag cgttcttttt gcagattcca atatgtatca tctgcctgat
    12361 ctgctgtaga aaaatgggta aataaacctt cccattctaa tacacgactt tctttgatga
    12421 atgcagctgc ctcttttact tcttcaggtg tacaaaatcc gatacgaccc atcccagtgt
    12481 ctgcttttag atgaatcgaa agaggcgtct ctagcttact tttctctaaa acagcagctg
    12541 ctgcttgcaa ccattcccgt gtacctgcag ttactgataa atcatgagca acagctacag
    12601 tcagtgcgtc gtaaggaacc atattcaaga taaggatggg ctgagtaaat ccagcttctc
    12661 tcaactcgac tccttcatct aaatttgaaa cacaaaagcc cgaagctcca cctgcaactg
    12721 ctgcctcagc tgtttcaata gctccatgac cgtatccgtt cgcttttact actgcaaaca
    12781 attcttttcc ttgcggaagt cttgcaactt catttgctac attttcagtt atagcttttt
    12841 tatgtatcac tgctttcgtt gggcggtgcc aagcgacaac catcgttttt ccttctttct
    12901 agtcttccaa aataatttgt gcaaaagctg tttcatttgt atgagtaata ctgacccata
    12961 cattgccttc atgaggtgaa cgcgtcacaa caggctgacc attttcattt tttaaaatct
    13021 cgacatcttg aaaggtaact tttccgatcc cagttcccca agctttagag aaagcctctt
    13081 tgcaagcgta acgcccgcct aaaaattcta cttggcgatg aaaaggcaag gactggaata
    13141 gtttcatttc atcagaggtc aagattcgtg caatgaattt tggtttttct tcgatcagcc
    13201 gagtgatcct aggaagttcg actgcatcga tcccaattcc ttttatcata tgcttttctc
    13261 ttttcaataa atttagaaca ctctcattct accaaattat aaccattttg ccacaaataa
    13321 gtccacaaat aacaaaaatg aaagtttctc ttaaaaaaga gaaactttca ttttatgttt
    13381 aatcgttatt tgaacggatt acaaatccgc gttttttatc attgtgacgt ttaccgccat
    13441 ctttttggct gttattattt ttgctataac cgccttttga tttgtttcca cgataattac
    13501 cgccgtcacg acgattacgg ttgtttccgc caccacgttt gccattttta ttgtatcctt
    13561 ttttctgcat tggcaatggg cgttctggtg tgatcttcac tggcactgca tcagctggat
    13621 cttttgctgt cgtttttagc aaaagtgcaa ctaaatcttg tgcagaatat tcttctaata
    13681 atttatctgc tgtttgcagg tatttatcta gaccattttc tgcaagtttt gtttcgattt
    13741 gttcaactgc agcacctaat tgacctttga atgcttcttt ttcggttggt ggacgtaaag
    13801 tcgtcatccg ttttttcgtc aagttttcaa tcacatgcaa gtagtccatt tcatttggtg
    13861 taacgaaagt cacagacata ccgccttttc cggcacggcc tgtacgtccg atacggtgaa
    13921 cgtagctttc tggatcttga gggatatcat agttgtacac atgagtgaca cctgaaatat
    13981 ctaatccacg tgcagcaacg tctgtcgcta caaggatatc taaatgaccg ctcttaaatg
    14041 aacgcaatac gctcattcgt ttttgttgag acaaatcgcc atggattcct tctgcacggt
    14101 aaccgcgtgc ttctaatccg cgtgccaatt cgtctacacg gcgttttgta cgtccgaaga
    14161 cgattgttaa ttcaggtgtt tgaacatcaa acaagcgagt catgatatcg aatttttcgt
    14221 attcttttgc gcggacatag tattgatcga tcaagtcagc tgtcatttct ttcgctttga
    14281 tcttcacatg atgtggattt ttcatgaatt taacgccaat attcttgata gctggcggca
    14341 tcgttgctga gaaaagtaat gtttgtcttt gatcaggaac ttgtgaaatg attttttcga
    14401 tatcttccaa gaagcccata ttcaacattt catctgcttc atccaaaact aatgtttgaa
    14461 cggttccaag tttcaatgtg tggcggttaa tgtggtcaag catacgtcct ggtgtaccca
    14521 caacgatatg tggacgatct ttcaagccac ggatctgacg gccaatatct gctccgccgt
    14581 aaacggcttg tacacgtact tttttgtcgc gccctaaacg atataattct tcttgtgttt
    14641 ggatagctaa ttcacgtgtt ggtgcgataa ctaaaccttg taattcgtga cgatctggat
    14701 cgattttttc taacattggt aatccaaatg cagctgtttt ccctgtacct gtctgtgctt
    14761 gtccaataac gtcttttcca gctaatgcta gtggaatcgt ttctgcttgg ataggcgttg
    14821 cttcttcaaa accagcgcgt tcgactgatt tcaacaattc tggtgataat tctagttctt
    14881 taaatttcaa atgaatcctc ctatatggtt gttaactgag gatgtagctt ttttgctacc
    14941 ccgtcgattt gttcggtcaa agtgtattgt ttctttttga cagtggccac gcacgttttt
    15001 atccattctt agcccctgtg gcaccaccga aaaatcactt tgcgaagttt aggcattaga
    15061 aaagccaaac gggtcgcgtt cagctttatc attattatga catctggctt caacaagttg
    15121 tttctatggc aaaaccagtc caatttctct tcatctttcg gcaataggtt attctaacat
    15181 agtttttcgt tttctgcaag tttacagatt tttatttcat ttgctgaagt tgttctacca
    15241 cttcaaataa gcccatgctg ttgctgcctt ttagcataat actatctgtt gcttcaagat
    15301 tttgtttgat agcatcaatc aatttattct tttctttttc aaaatagaac acaggtagtt
    15361 gggggtattt ttggttcaat tcttctttta gtgcatgcat ttgttcccca taaaggaaaa
    15421 tcatggcgaa tttctcagga tcgatatgtt cactcatttg cgcatgcata tttaaagact
    15481 ctttccctaa ttccagcata tctgctaaca cagcgattct gcgtcctttt gttggcagat
    15541 ttttaaaagt atctaatacc agtcccatgg ctgttggatt tgcattgtag acatcgctca
    15601 aaatagctgc accatttcct gccgttagcc attcggttct gttttgagtg atagaaacat
    15661 gggcgagccc ttggcgtatc tcaggtatgg acagtccaaa gaagcgcccg atcgtatacg
    15721 caatcaaagc attcgttaca ttataacttc caggcagcgg gatcgtaaat gtttctcctt
    15781 ctactacaaa atcggtttgt gcttttcctt ttgctgtcac ttccgcatga acatcgccat
    15841 ttccaatacc gaatgttgtg actgtttgta ctaatgttgc cgttaaaggt gtcagcaacg
    15901 gttcatctgc gggcacaacg agtaatccgt ctgatacaag tccgtcagtg atttccattt
    15961 tggctttagc gattccttct cgcgacccaa gattttctat gtgtgcttca ccgatcattg
    16021 tgattgctgc agcttttggt tgagccataa gtgaaagttc cgtgatttca ttggcgtgat
    16081 ccatgcccat ttccaaaacg atcttttctg tatcttgggg catgtgcaaa atcgtgtaag
    16141 gaagaccaat attattgttg taatttcctt gcgtcttata tgttttgtat ttttcagaca
    16201 agacagacgc ggttagatct ttcgtggtgg ttttaccatt gcttcctgta attgcaacga
    16261 cttctgcgcc tactttttcc aagtaatagg ttgccaattg ctgcatggct ttcaaaggat
    16321 ctgctacagg aataactgga aaattttttg gagcttgttc aacaggcaag ctccaaaatg
    16381 aagcagatgc tccgtttgtg atcgcttgct ggataaattc atgtccatct cgcgtaccag
    16441 caagaggaac gaataaactt ccttcggtta tcttccggct gtcaaattcg acattggtaa
    16501 tctttgtcag tgcagagcat tcgctgccca cggcttgtgc aatttcttgt attgttagat
    16561 tcattgagat ctctcctaaa aaaggttgtg aaaaaagcgt ttggcctaaa cggcgggttc
    16621 gcatgactct cacttccaag taaatttctc tattcgtatt tagaagctag aatgaacgcg
    16681 gttacgcaat cactccagcc tcttctttat ttctctttat tcattttttt caaaaaaaga
    16741 ttgacgctga tggtatcgat tcattcctaa ctggatcagt tcttcaatca aatcaccgta
    16801 tttcaagccc atattttccc ataagagtgg gtacatactg aactccgtaa atcctggcat
    16861 agagtttaat tcattcagga ataattcatt tttatttgtc aaaaagaaat cgcaccggct
    16921 caatccgctt ccacctaaca tcgtgtaagc taacttcgcg tactcttgcg ctttttgata
    16981 aacttcttct ggcacttcgg ctggaatctg catttcgatt ttattattga tatattttgc
    17041 ttcataatca tagaatgcta cgtcttttac gacttcacca ggcaaagtcg tccgaacgtc
    17101 ttcatttcct aatacagcaa cttcgatttc gcgcgcttca attccttgtt caacgattgc
    17161 tcgagaatca tactgatagg ctgttgctaa agcattttgc agctcttctc ggttttctgc
    17221 ttttgtaatg ccgacactag aacccatatt cgccggtttg acaaacatcg gataaagcaa
    17281 agaaccttca cattgatcaa atactttttt aggattttct ttccattgat tcttaagtac
    17341 tggtacataa ggaacttgcg gcacaccagc agcttgtaaa atatacttgg tcatgatttt
    17401 atccatggca catgcactgg tcaatacgcc tgcgccgaca taaggcatat tcaatgtctc
    17461 taagaagcct tggatcgttc catcttcccc gtttggccca tgtaaaactg gaaaaacgat
    17521 ggctccttct tctttgattt cgcccggatt gatcactttt cctgtaaagc cttcctctgt
    17581 ctgtccactt gggtcccatg aaagatgcaa gacatctttg ctggcaggtt tttctgttag
    17641 taatggacct ttgacccatt gtccttcttt tgtaataaat acgagttgaa cttggtaata
    17701 attataataa atggcattta aaactgaaaa tgcggaaaga atggacactt catgctctgc
    17761 gctgcgtccg ccatatagta aagtaatctt caaaagattg tcctcctgta ttctaaatcg
    17821 ttcagtgatt tactctcata ttttagcaca aaacaaaaat aaacgaataa cttatttata
    17881 aaaaaacgag ccagcttcta aaagaacgag cgtcatttat tttttgtcag ctagattatt
    17941 tgtcccgttt tttataaaaa gttatgataa agaaaaggag ggatgatgat ggcgcaaaca
    18001 aatccacgca gccgcttggc agaaatcatt cgagtgttca tcaattacaa tgtagtgcca
    18061 aactttgtac agcaaaaaaa tcccgaacag gtaaaaaaag cctttgaaga actaggcccc
    18121 accttcatca agataggaca gatgctttct gttcgagaag atttgctttc ttctgccttc
    18181 actcaaacat tcaaaacatt acaagatagt gtacctagcg acacattttc aacggtcaaa
    18241 aagacgatcg aaacagaact ttctctttca ttatcagata tattcgatga tttttcaaaa
    18301 tcgccttttg cctctgcttc tatggggcag gctcatcgtg ccaagctgaa gaacggtgac
    18361 gcggtagtcg ttaagatcca acaccctaac atcgccgaag agatccgact tgatcttcaa
    18421 ctatttgaac gagcgatccc tttgatcaaa tacattcctg aaactagcgt agttgatcta
    18481 aaaggggtac tccaagaagt aaaacgttcc ttgatcaatg aaatggactt cttaaaagaa
    18541 agtcaaaatg gcgaacaatt ctatcaaaag aacaatggct ggaaagagat ccgcagcccc
    18601 aagatttatg acgctttttg ttcgaaaaaa gtgatcgtga tggaagaaat gtctggtaaa
    18661 aacttgaatc atttaatgaa cgccgaaaac aaaactgaaa cttttatcac tggaattcaa
    18721 aacaagcagc tgaaacaaga agttgcaaag cttttagttg agaactttat gaaacaggtg
    18781 tttgatgatg gttttttcca tgctgatcct catccaggga atctgctatt ccatgtacta
    18841 acaaaagaag aacagacaca agctagccga aaaactgaaa cagtccatga aaaagagttc
    18901 ggttcctttg cttttcgagc ctccacttct gcagaagaac ctgttgcgcc atataccatc
    18961 aactacatcg attttggtat gatggggcat ctttctgcag gacttaggca aaaactcaca
    19021 caagccgttt tagcgctgta tacgaaagat gcttatcgaa tcgaaaaagc ggtccttcga
    19081 ctttgtcagc aagaaggctc gtttgatgaa agtcggttcc accaagaact gacaagtttt
    19141 cttgaacagt attatgattc tcctattgat gaaatcaact tgcaagaagt attcacacat
    19201 gtcgtgacga tttgtcatca aaataatctg cagttcgatc gtgatatcac gcttttgctg
    19261 aaagcatttg ggacattaga aggggtgatc cgcgtcctcg atcctgaagt ttccctcatg
    19321 gaagtcgctt cgccttttgc tcaacattat tttctcactc atctggatgt agaagataca
    19381 ttgaaacaaa gcggattaga cttattggaa gggatgaagg ctgcgcctaa gatccctcaa
    19441 cagctccacc atctattaga aatgtggact tcgggacaag gaaaagtcaa tttggaattg
    19501 aaaaagcaag acaaattatt gagcaggatc gaaagtatga tcaatcggct tgttttcggt
    19561 atgattttag ctgcattgat cgtagggtcc tccctgcttg tccaagcagc acctgtagaa
    19621 aacgcagagg tcgtttcctt attagggata tttacgtatg ctattgccgc attcgtcatt
    19681 atttttttgg caatcgatgc attgatccaa atgtataaga agaggaaaaa gtagaaaaag
    19741 gttgtgacag aaagaatcag cttcaagaaa taagaagggg ttttacataa attgtttctc
    19801 aaatttttgt gaaaattcgg cttatttttc gaagaggcta cttctgaaac accgagaata
    19861 aaaaatgtga aaaagctgtt ctgcacccag taataagaac aatcaaaaag ggaacatgga
    19921 acacatttat cctgtttccc tgttcccttt ttgatgtatt cgagcataaa taaaatacct
    19981 tcgttaccta ctatcttctg tacttggtaa accttagtca attgcctttt ttaacgcttg
    20041 acgaaaagac atatgatctt tctccgtaaa gggttttgga ccctttgtcc ttatgccagc
    20101 ttttcttaac tttccactat gataacgttg ttctagaagc gtgccgatat tcgcttcgtc
    20161 aaattctttt ccagagtgat ggaacactcg cacacccttc ggcaatacaa gtgaagctaa
    20221 accataatct tgcgtgatca aaaagtcatt cttcttaacc ataccgacga ttttataatc
    20281 cgcactatca gctccgcggt ctacgtagac gatttggaca tgattagcgt attctttggt
    20341 cgtataatgg tccacactgg tcacgataac cacgtcaaga ccataggatt ctcctaattt
    20401 tatgacatct tcttttactg gagaaccatc tccatcaatg aatagcctca ttctttttcg
    20461 ccttcttcca taaatgataa acagttacta atccgctcag taaaagacca acattgatcc
    20521 attttgccaa gataagcagt tgtacacttt ctatcggcac tgattcatgg ttcagcgtga
    20581 tcaataatag atggattggc tctcccattt cttttgctaa aacaaacaaa agcgtcatca
    20641 ataaaaactg gtggatcaca ctccctttat tttgagtagt atacttgaca acgatccggc
    20701 gatccattgg aatagctgta tcagtttgat aatattcctt atttagccag ctgcctgtta
    20761 ctacgtgcca gaaactaagc agaaagacac ctatcgcaaa accatttgga atcaagagga
    20821 ccaataaaga agaactcagt ccaagcaatt gcatagccaa tggagaccat gttaaacgtt
    20881 tcaaaatcca tggtccgact atttttgctg caatcaagcc tgctgcataa ggcaaataca
    20941 taaaaatcga aagtttttct aatccgtaca gtatgccaac ataaaaactg ctaaataaaa
    21001 aaaggaaatt ccctagcatt ccatttagaa aagtaaacag atttagccag cttggcaact
    21061 tccattcctt cttctggtgt cctaaaacaa acacgaacaa gatgaacagc agcaagaaac
    21121 cataaatagc aagatcaaaa agcctttcat cgaataacaa tcgggcattt ctcaaaagaa
    21181 acaataagcc aaagaatatc aaaaataaaa caagctctcg cgtagctacc agatttcttt
    21241 tgatgtcttt gaaatccagt tcgtaacgcg gataatgact aaccgtatgg taggccatga
    21301 cgtaaaaaag tgtataaata gtaaagcttg ctgcaatctg agtcggtgca ggcaactcaa
    21361 tcgcttgata caggagaaat agccagatag ctgcaaataa gtactttttc ccatccatat
    21421 tgataaaacc ttgcgtcttt tcgtgaaaat tgaccgtggt attagcaggt acaagccatg
    21481 ctgcactcag ccctagcaaa acacttgaaa aataaaataa aggaaaataa aatacgccta
    21541 atattccaag caatgaacca gcaccaccaa tcagcagagc gatcatcagc aacgtgtaac
    21601 tatctagacc aaatcgtaga ctgttgacta aaaatagtcc agtcatccga aaggcataaa
    21661 acagcgtaaa tggcaaaatc gtcagccaat tatgcgagtc tgttagtccc aaaaataaaa
    21721 gataaggaat aaaaataacc gtgttactta agataaaggg cgttgtttgt gtaaaattac
    21781 gcccaaatcg tttgagtttt tcgaacatga caatggctcc ttaaacagtc ttataactaa
    21841 tctgctcttc gattttagca ttaaagggga aataaaccaa ttttacagaa tatattcttg
    21901 taaaaaaaaa tacagaaaga agcataagct tctttctgca tttttacggt acttgattac
    21961 tcaagcagac aagcccttct ccacaaaagt cttgcccgac acccctgcat ccggcaggtg
    22021 ataaagatag aaatcaatta tcctgtgcct ccaaaatcaa ttaggagctc tgtaaaccag
    22081 agtccttctc gacccgtcgc ataaatttac tttaacacag ttcctatgtt tatgcgagag
    22141 cttttttcat tttataatcg accatttgaa atccttgacc gatatctgaa ataagttctt
    22201 ctgtaatctc aaatccttca tgcttataaa cctcgattgc ttgatgattc ccctgattca
    22261 ctcgcaaaaa gatctctctt tcctttgttt gcgcagcaag ttcttcatac cagcgaaaaa
    22321 ctgaagaagt caatccttga ccacgtactt tatttagcaa atataattta cttaaataaa
    22381 tttttccatt tatctcttca taagcagtgt aaccgacagt cttttcttcg ctttttaata
    22441 aaaagtagga cacaccttct gtcagttctt tttgaatctg tttttttgac tgataagttt
    22501 tcagcatgta atccacttgt ttttgaccaa tgatcggcac aaaagcttcc ggccatactt
    22561 cttgtatgac tgccatcagc tcatccagtt cattttctgt tacttttatt ttttccatac
    22621 acccacgtcc ttttttacat tataaatgac cttgcgcttg atggaataaa aagagcatga
    22681 tcat
//
LOCUS       DACEPW010000027        21995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEPW010000027 DACEPW010000000
VERSION     DACEPW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.027"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2258
                     /gene="ileS"
                     /locus_tag="IW819_000823"
     CDS             <1..2258
                     /gene="ileS"
                     /locus_tag="IW819_000823"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1243946.1"
                     /translation="PIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGKDILDEDT
                     AFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIGWENVTVL
                     KEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYIAGSKYKL
                     SVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSYPHDWRTK
                     KPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWVISRQRAW
                     GVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTHPGSPNGE
                     FTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSITTSVAING
                     VAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVDYEADVRV
                     SMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVRLNQVIKE
                     IREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCMQTVFYQT
                     AVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTWTAFMDFR
                     DNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSPDYFEVMA
                     ADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCAKIVEDFY
                     PEAVAEGFEEK"
     gene            complement(2307..3833)
                     /locus_tag="IW819_000824"
     CDS             complement(2307..3833)
                     /locus_tag="IW819_000824"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1243947.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(3848..4495)
                     /locus_tag="IW819_000825"
     CDS             complement(3848..4495)
                     /locus_tag="IW819_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1243948.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(4679..5008)
                     /locus_tag="IW819_000826"
     CDS             complement(4679..5008)
                     /locus_tag="IW819_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1243949.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            5185..5913
                     /gene="sfsA"
                     /locus_tag="IW819_000827"
     CDS             5185..5913
                     /gene="sfsA"
                     /locus_tag="IW819_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1243950.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            5929..6942
                     /locus_tag="IW819_000828"
     CDS             5929..6942
                     /locus_tag="IW819_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1243951.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            6942..8219
                     /locus_tag="IW819_000829"
     CDS             6942..8219
                     /locus_tag="IW819_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1243952.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            8282..10984
                     /locus_tag="IW819_000830"
     CDS             8282..10984
                     /locus_tag="IW819_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1243953.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            11136..11453
                     /locus_tag="IW819_000831"
     CDS             11136..11453
                     /locus_tag="IW819_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1243954.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            11483..11803
                     /locus_tag="IW819_000832"
     CDS             11483..11803
                     /locus_tag="IW819_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1243955.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            11911..13371
                     /locus_tag="IW819_000833"
     CDS             11911..13371
                     /locus_tag="IW819_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1243956.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            13439..13660
                     /locus_tag="IW819_000834"
     CDS             13439..13660
                     /locus_tag="IW819_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243957.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            13691..13873
                     /locus_tag="IW819_000835"
     CDS             13691..13873
                     /locus_tag="IW819_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1243958.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            13873..14286
                     /locus_tag="IW819_000836"
     CDS             13873..14286
                     /locus_tag="IW819_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1243959.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            14409..15590
                     /locus_tag="IW819_000837"
     CDS             14409..15590
                     /locus_tag="IW819_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1243960.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            15979..16052
                     /locus_tag="IW819_000838"
     tRNA            15979..16052
                     /locus_tag="IW819_000838"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16013..16015,aa:Arg,seq:cct)
     gene            complement(16121..17260)
                     /locus_tag="IW819_000839"
     CDS             complement(16121..17260)
                     /locus_tag="IW819_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1243961.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(17559..18194)
                     /locus_tag="IW819_000840"
     CDS             complement(17559..18194)
                     /locus_tag="IW819_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243962.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(18307..18942)
                     /locus_tag="IW819_000841"
     CDS             complement(18307..18942)
                     /locus_tag="IW819_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1243963.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(18976..19437)
                     /locus_tag="IW819_000842"
     CDS             complement(18976..19437)
                     /locus_tag="IW819_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1243964.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(19567..19998)
                     /locus_tag="IW819_000843"
     CDS             complement(19567..19998)
                     /locus_tag="IW819_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1243965.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(20016..20336)
                     /locus_tag="IW819_000844"
     CDS             complement(20016..20336)
                     /locus_tag="IW819_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1243966.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            20635..21411
                     /locus_tag="IW819_000845"
     CDS             20635..21411
                     /locus_tag="IW819_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1243967.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            21426..21629
                     /locus_tag="IW819_000846"
     CDS             21426..21629
                     /locus_tag="IW819_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1243968.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            21645..21983
                     /locus_tag="IW819_000847"
     CDS             21645..21983
                     /locus_tag="IW819_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1243969.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT         7066 a         3760 c         4262 g         6907 t
ORIGIN      
        1 aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa attgaatata
       61 aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc aaagatattc
      121 ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg ccagcaaacc
      181 aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat ggccgtaagt
      241 ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa
      301 cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt catccattct
      361 atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct ggtactggat
      421 tagtccatac tgctccagga catggggaag atgactatat cgctggtagc aaatataaac
      481 tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca cctggatttg
      541 aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa gaaaaaggag
      601 ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg acgaaaaagc
      661 ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc cgtcagaata
      721 ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca cgtttgtata
      781 atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac
      841 ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca atcgaacatg
      901 tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac
      961 ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag
     1021 atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta cgtcagcgac
     1081 cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat cgtggctggt
     1141 tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac aaatcagtta
     1201 tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct ttaggaaaca
     1261 tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc ttatgggtat
     1321 ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt caagtggcag
     1381 aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca gaagatttcg
     1441 atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa tatatgaccg
     1501 ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa tataacttca
     1561 tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca ttctacctgg
     1621 attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt cgttgcatgc
     1681 aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct atcattccgc
     1741 atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt cagttaagcg
     1801 agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc tggactgctt
     1861 tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac agcaaattga
     1921 ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt cgtgaaatgt
     1981 taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac tactttgaag
     2041 ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat gttgctatta
     2101 cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag
     2161 gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct
     2221 atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat
     2281 gttctttgtc acaccccctt ttgttatcaa tcgtttaatt ttccacgttc acgataccag
     2341 ttatctggtt gccagatcca ttcgaatccg tctttttcaa gtagttcgaa agctgcttta
     2401 ggtcccattg atccagcttt gtagttggga aaatccggtt gtgtttggtc ccaagctttt
     2461 cgaatgacat ccacgatatg ccaagattga gcgacttctt cccaatgaga gaagttcgtt
     2521 ccatcaccat tcaacgcatc taatagtaat ttttcgtaag cttctgggct attttcaacc
     2581 atttctgcac tatttctaaa ttccaatttg attggttccg tttcaaatcc ttgccctact
     2641 tctttcccat tcaaggataa agaaaatcct tcagttggct gaatatagat cgttagaaca
     2701 ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag atgttttgaa tacattaaca
     2761 ggaacttgtt tgaatacgat gttgatccga gtcccttttt cagtcaaacg tttgcctgta
     2821 cgtacataga aaggaacacc tgaccaacgg aagttatcga tagtgaattt gcctgcgacg
     2881 aatgtttcag tctctgattg agcatccact ttgtcttctt cgcgatagcc cttgaattgt
     2941 acaccatcca attcaccttg agcatattgc ccgcgaacga agttttctaa cacttcttct
     3001 tcgttataaa tgcggatagc tttcaatgct ttgatctttt ctgtacggat ttctttttcg
     3061 gaaaatgcta ctggcggttc cattgcaagt aatgctacaa cctgtaaaat atgattttgc
     3121 accatgtctt taagtgcgcc actatgatcg tagtagcccc cgcgatcttc tacaccgagg
     3181 ttttcgccaa aagtaatttg gatattgtcg atatagcgat tgttccattg agcctcaaaa
     3241 atattattgg caaaacggat agctgagatg ttttggatca tttcttttcc taaataatga
     3301 tcaatcctga aaatatcttg ttctgggaat acttctctga tttgctggtt caattcaaaa
     3361 gcagactggt aatcagaacc gaacggtttt tctatgatca aacgattgaa gccatttttt
     3421 gtcataatat tttgtgattt caaatgagaa acgatcgttc caaaaaattg aggtgccata
     3481 gccaaataat agacacggtt accgtttaat tgatagtgtc catctaatcg gtcagacaat
     3541 tcttttaatg tctgataatg cgttgaatca tttacatcat gggattgata gtaaaaatgg
     3601 cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg cacggattgt ttctttgaca
     3661 acttcacggt agtgctcatc gctccaggga cggcgagcgg ttccgattac agcaaaatgc
     3721 tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg ggtacaattt acgctgggct
     3781 aggtcacctg taccgccgaa tatcgtaaac aatacatttt tttcattaga catgtggata
     3841 cactccttta tattttatcg acaaaaatcg tgctggccgc cttaaagctg acggggattt
     3901 tctttctatc attttggata gtgatcggtc cttcataagc tgcgatgtca acgatctcat
     3961 attcttcgtt gatattcagg tcaatagtga ctaagtaatc caacaattct ttttcatcaa
     4021 gcactcgtgc gatacgcact tttgtaccga caggatagga ctccaatgtt tgtcggcgtt
     4081 cttcatggac aacttcctct tttttaggaa tcgatcctcc atgaggacaa tgttcaggat
     4141 gattcaaata gttttctaaa tgatctgcaa gatgttccga agtcacatgt tccaatactt
     4201 cagcatcatc atggacttca ttccagctat agtctaaatg ttcaactaaa aaaacttccc
     4261 ataagcgatg tttgcgaatc agactgctag ctttttgcag tccgcttgca gtcaattgta
     4321 caccttgata aggggaatgt tctacaagct cttctttcac aagtttagaa atcatttcac
     4381 tgacagatgc agctgagaca tctagactag atacgatttg tttattgttg actttcgttg
     4441 tatctccacc tagttctaaa atgattttca aataatcttc tcgatttggt gtcatattcg
     4501 atcatccttt catggtaaaa aaccatcatt agtaagtgta acaaaaaaaa cgttaattga
     4561 ctatttttct aaataaaaaa caaaggattt ttagacgttt ttacaacaaa aaacgaaaga
     4621 tgatagagaa gactcattct ttcgtttata aaggatatat atatacattg ggagggatct
     4681 atttttcaga taacaactta tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg
     4741 gataagaaaa gataagataa aaaccatact gatcacgtac gactaaacga tattcatcta
     4801 tttctaccac agatgtgcca agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa
     4861 tctgtaattg ttgtttttgc tggctatcac taaagaacca tgtaccttga tactttgaga
     4921 gtccttctga cttttccgct tgttttttct ttttcaagta aataaaactt gagagaacgg
     4981 taatgagtga accaagaaaa tagatcattt tttttatcac caaatcactc ctttatgaga
     5041 aaataatcgc atagacatat tctttcttct ttatttcatt gtaccgaata aaagctaaaa
     5101 tttctctaag actttcgact ggttttttta ctgtttttct acaaattatg ataggataca
     5161 tagtaggaag aaaggtgaca taatttgaag tatcagcaag tggaaatagc tttttttatt
     5221 gaccgcccga atcggtttat agcacattgt atgaaaaaag acggagaaat aattattgcg
     5281 catgtcaaaa atacaggtag aggaaaagag gtcctgatac caggcgctga agtggctatt
     5341 gtatttgctc ctggacctaa acggaaaacc gcctatgatc tgattgctgt aaaaaagaag
     5401 ggtgactggt ttaatatcga tagtcaattg cctaatcggc tggcaattga tggtatttta
     5461 gacggcacga tccttttacc aggattaact ggtccattta cggtatacaa gcgtgaagta
     5521 acttatcttc actccaaatt tgatatttat ttagagacgg tggtcggaca aaaagcattt
     5581 gttgaagtca aaggtatgac acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc
     5641 acactcagag gattgaaaca tataggagaa ttaacgtatg ccgcacaaga tggttatgcc
     5701 gcttatgtct tgtttattgc tcagttcgaa catcttcatc tggcaacgat ccatgaagaa
     5761 atgcaaccag cactagctga tatggttcga catgcgcagc aaagcggagt tcaaatcctc
     5821 gcctataatt gtcaagtatc ggcagatgag gtaactgtca agcaggccat tccatttgat
     5881 gtagatatcc cttttgaaga tcctatacaa tagaaagaag gtaaaataat gatcaactta
     5941 ggtattatcg gaacaaattg gattactcat caatttgtcc aagcagcttt aagcacaaaa
     6001 aaatatgaat tgacagcggt atattctaga catttggaaa aagcacagga atttggttct
     6061 cattatgaag gggatatcga attcgcaaca gatttgaaga cttttttcaa tattactcat
     6121 atggacacca tttatattgc ttctccgaat agtcttcact ttagtcaagc taagcaagca
     6181 attttggcag gaaaaaatgt gatagttgaa aagccagctt tttcgacgcc aaaagaaatg
     6241 gatgaaatca tccatttggc aaatgaacac aatgtgtatt tttttgaagc agcaagaaat
     6301 atccatgaat caggttttaa aaagattgcc gattacttac cattaaaaga cgagataatc
     6361 ggtgcaaatt tcagctatat gaaatattct tcgcgctatg accaagtatt agatggagaa
     6421 gaaccgaata tcttctcgac tcatttctca ggtggtgcat tgatggattt aggtgtttac
     6481 cttgtatatg cagctgttgc ttggtttgga atgccaaaag aagtccatta tttcccaaga
     6541 aagatttcta ctggtgtaga tggtcttgga tgggggatct tacgttatga gaattttgat
     6601 gtatcgatcc agccaggtaa aatcggtgat tcttatttac cgtcagagat ttattttgac
     6661 tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac aagcacaatt tcatgatcgc
     6721 gcgcataaag aaatcattga tttggcggta acagctgcag aaaatccaat ggcagatgaa
     6781 gcagaggatt ttgccaatgt catggctcat ccgaaagatc cagcttgggg attgttgtat
     6841 gaagagtgga tcgagctagc tagaaatgtg aaccaagtga tttatgattt aagaaaaaat
     6901 ggcggcatca aattcgatgc agataatgag gaagattttt aatgaacgaa tttgtacaag
     6961 tatttcccga aaattggcag aaaaaatatg aggataaagg gtttatctca ccttctctta
     7021 ttcaacgagc agtttttcag ccactaagta ataaacaaag tatagtagcg gtttctccta
     7081 caggttcagg taaaacactg gcttatcttt ggcctctact actaaacgtg gaaccaggtg
     7141 aagcaagtgc gttggtcatt tttgcttctt ctcaagaact agctattcaa gtagcagatg
     7201 ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca atctttggtg ggcggagcca
     7261 acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag
     7321 gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg
     7381 tatttgatga agcagaccag ctatttgacg ctgggaacag ccaaatcatt gatcaaattc
     7441 ttcatcaagc tccaacagag tatcagctag cttttttttc agcgacagca gatcgttccc
     7501 ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac aattgacgtg acagcagagg
     7561 acgattctcg aaaaggactg agacattact ttttacgtgt accgatacga aagaaagatg
     7621 actacttgag acgtctcacg catatagaag attttcatgg tctgattttc tttaatcaac
     7681 tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc
     7741 tggcctctga tcagaacaag ctactccgaa aagcagcgct agaacaattc aaaaagaaaa
     7801 tggtcagagc acttttgacc acagatgtgg cggctagagg tttagatatt acaggtcttc
     7861 cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa
     7921 gaactggtcg aatgggtaat gaaggaatag taattacact cgtacaagat aataatctaa
     7981 gagatttaaa acggctttta cgtccatcaa atataacact tgaagagatt tttttatacg
     8041 gaggtaagct gcaaactgag cagccaacca aagaagatgc agctcctgca gtaaataaaa
     8101 agtatgcgaa aactttttct ggaaaggagc agaacccaga atataacaaa gaaaaaataa
     8161 gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa aggagcaagg cgcaaataag
     8221 cgccttttta tgctatgatt atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta
     8281 tgtgaaagaa cgtcaagaag agatcttgca gctattgaca gaaaaccaac aaggattgac
     8341 agcaagtgat gtagctgagc gcttaactat cgatcgaagc aatgcaagcc gatatttaag
     8401 tgaactatac aaggcccatc atattgtaaa aaccgctggc cgaccagtcg tttactcttt
     8461 gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt
     8521 tgaaacactc gttggagaaa atgattcttt aaaagtcagc atacagcagg caaaagctgc
     8581 gattctttat ccaccgcggg ggttgcatac gattattttt ggagaaactg gtacagggaa
     8641 gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat tcagagatgc tttctgcaga
     8701 tgcgccattt gtttctttca attgtgcgga ttatgctcaa aatccgcaac tgctctttgg
     8761 acacatcttt gggatcaaaa aaggggctta cacaggagca gctcaagata gtcccggtct
     8821 aatagccaaa gcagacggcg gtatattgtt tttagatgag atccaccgtt taccgccgga
     8881 agggcaggaa atgctgttta ctttcattga taaaggtatt taccgtccgc ttggagaaag
     8941 tgcacaagta catgaagcat ctgttcagat cattggggcg acgactgaat cttcagaaag
     9001 tttcttatct acgttcaatc gacggattcc tatggcaatc accttgccga atcttgcttc
     9061 acgttcactg gatgagcgtt atgaaatcat ctctcttttt atcaaacaag aagcgaatcg
     9121 tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt gccttcatgc tttatgatgc
     9181 ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt
     9241 acattaccgt actcatcatg agaaaaagct aatcattcga aaagaagaac tttcccttca
     9301 agtacaaaaa ggattactga aaatcaagga aataccggaa cgtctcgacc gattcatgga
     9361 cagcaaaagt cagtatttga catttgaacc aggatttgct gatgttgtct ggtcacaaga
     9421 tccagaacga aacatgcagg tctataacga tattgaggaa aaagtcactt ctctttccga
     9481 aaccggtgtg gagaacattg atctagagac cctcatttca aaagatgtgg atgcttattt
     9541 ccagacatat gtcaaagaat tgactaaaag ttcgattccc aaagatcttt tgccagatga
     9601 aatctggcgg ttaaccaatc aattgtatga tgtggctgaa aaacggttag accgtattta
     9661 taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa agtacattgg atcgtgtgag
     9721 agaaggtcat atgatcgtcc acccagattt gaataacgta cggaaaaatt ttaagaggga
     9781 attccaggta gcaatcgatc tttcaacgat cattgaagaa gaagaacaag ttgagattcc
     9841 ttttgatgaa atcggcttta ttagtatgtt tctttctatc aatctaggag aaagtgagcc
     9901 tgttaaagaa aacaaagtag atgttctcat attgatgcac ggcagacaga ctgcttcaag
     9961 catgttggag atggcacaag aactattagg aacaaaagta gggaaagctt ttaatatgcc
    10021 actggttatg gaagttcaaa caatgtatga agaagtgtta caatatgtca aaaactatca
    10081 agagcatttg acgaatggcc tactcttgtt aacagatatg ggatcattaa acaccttcag
    10141 tgatttgatt tatgaagaga ctggcattcg gacgaaagct atcacgatga caagtaccat
    10201 gattgtgata gaagcgatac ggatggcaag tgttggaaga agcttagaag atatttatca
    10261 aaatatccag ctttcctttg aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga
    10321 aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt actggtgaag gagtagctgc
    10381 caaattatat cagcgtatta tgccagtgat taatcaaaat aaggtagaac tgatccagat
    10441 gcagttttta gaacgtgaat cattcaaaaa acacatcgat gccttattgg aagaatatga
    10501 gatcaaagca attgcgggaa cagttgatgt tgattatcaa aatatcccat ttttttcagc
    10561 ctatgacgtt tttgatgatg aaaaactaaa tattttaaag cgtatagctg atgatgagat
    10621 cccgacagct aaaattgtcg attcacttgc tggatcatta agcgatgtct tttctattga
    10681 tgaactgatc cgttcattgc aaaagacagt tcagcagatc cagaccgatt tgtcgattat
    10741 cgtggaacct ggagtagata ctggaatcgt tatccattta gccttcatgt tagacggatt
    10801 gatcaaacaa gaaagccatc ggcaatttga gaaactgtct attttctcaa aaatttatcg
    10861 tttggaaatg gatatcgttc gggcaaactt gatggcacta gaacgaaaat atcaggtaaa
    10921 aattccagaa gatgatatcg catttcttac acaaatgttt cttgaaaata aaataaaaaa
    10981 atgaacacac taatcatact gtttacacac taaacatctg tgtgcgcact ataaaaaaga
    11041 gctaaaaagc atgaaaacgc tatttcagct ctttttttta ttggcatgct tcttgcgtta
    11101 tataaaagtg taaaaaaata aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag
    11161 tttgttcagc agggatgagt acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag
    11221 aaaaagggat ggaagccgat atttttgcgg tctctgcctc agatgcagat aataatttag
    11281 aatctaagaa tgtcgatgta ttattattag gtcctcaagt acgtttcatg aaagctcaat
    11341 ttgaacaaaa acttgcgcct aaaggtattc cattagatgt catcaatatg caagactatg
    11401 gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt
    11461 atgcagttgg gggtataaca acgtggaaga tcaacaaaat ctagaagcca tcatggggtt
    11521 aatcatgttt ggcggtaatg caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat
    11581 cggtgatttt gagttagcgg ataggaaaat cgctgatgca gaagaatcac ttatacaagc
    11641 gcatcatgca caaacagaca tgttgacaca agaagcacaa ggaaatcata tgcaagtgac
    11701 attattgact gttcacagtc aagatcactt aatgacatct attgcgttta ctgatttagc
    11761 aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa taaatacaac ttcttttttg
    11821 aacggaagaa gaaaaatttt tgaacggatt ttttattttg ttataaggag aactctcttt
    11881 atataaaaaa cttataggag gaagtaaaaa atgaacggac tatcaagttg gatggagaaa
    11941 tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat
    12001 gcttttatcg gcacactgcc cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca
    12061 attattcgtg atttaccgca acagtttaac agtacctatg ctgacagttt agcagcagac
    12121 aaaggaatct ttgccgtatt gaatgtcatc atcggaatca atggttttgt ctggaacggt
    12181 acactagcaa tcgccggatt gatctttgcc ttttcttggg ggtataatct tgcccgagct
    12241 tatggagtaa atgatctagc tggggggatc gttggtttag ctaccttgat tcaaggtatt
    12301 tcctttggtc cagcattatc agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat
    12361 gcaatcaata gttctgagat tggtgcaacg attgcagatg gcacactgtc agttggatta
    12421 tggggttggc taaaactaga tcatttgaat ggaaacgctt attttacagt tatgatcatg
    12481 ggagctattt cagtcattat tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg
    12541 cctgattcag taccaccagc agtatctaaa gcatttgcag ccattttacc agcgacgatc
    12601 gctttatacg tcattgctat tatcaattat atcgtaggtc gtgtagctga tgggcaactg
    12661 atcattgatt tggtgcaaaa atatattgca gaaccattcc taggattatc tcaagggttg
    12721 ggagcagtat tgatcgtcac agtatttgtt caagtattct ggttctttgg tattcatgga
    12781 ccaaatgtat tggcaccagt gctagaggga atctggggac aagcacaact aatcaatatt
    12841 gatattttcc aaaaaggtta taacggaaaa actggtacac cagccgtgtt agatgcaatc
    12901 gaagatggaa aagcttatat gtgggtccgc ggatcgttcg atgcttttgc atggtttggc
    12961 ggatcaggcg gaacgatcgt cttactgata gctatcttga ttttctctaa acgtgcagat
    13021 tatctaacag tagctaaatt atccattggg cctggtatct tcaatatcaa cgaaccagtg
    13081 atgtttggtc tgccaatcgt attgaatgca atcatgttta tcccattctt gattgctcca
    13141 gtagtagcaa cagcaattgg atggacagct acttatcttg gcctagtcgc tccggtatcg
    13201 cagcaagtca catgggtagt accgccgttc ttattgtcat tcttagcaac aggagcggat
    13261 tggcgggcac cgatcgtaac attagtatgt atggtcgtga ctttcttgat ttgggcacca
    13321 tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg aacaacaata agatgaacgt
    13381 gaggagcggg acgctttaag tttcgttcct cataatgaat tttctaggag gaactgaaat
    13441 gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa
    13501 tgaagaagga atgactttta catttaaaga aaaacaagga atcaagctgt tctttgaaac
    13561 aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag gaactaatca aagcacagcc
    13621 ttggggaaca gtcctttatt ttcaatcaac agctgtttag aaaatggttt aaaaagatag
    13681 gatggttgag atgataaaaa acggtttatt tttatgtagt attggttctt tgatcgtgtt
    13741 gtatagtttg aatccacatt cgcaaaggta tgatttgtat agtatggtca caggtctttt
    13801 tctaacagga ttaggaatct atttcttcat gaaggggaaa aaaagagaag aagcaaagga
    13861 gaaaaataac taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat
    13921 aaagttatgg actctgtcct tttcgatgta cgtaaagcga ccggcaaatc agtaactaga
    13981 aaacagctga aaaatttgga gtacgtcaaa caattctcgc aaaataaccg ggctcgcata
    14041 aaaatcgaag aagtcattga aaaccagtct tataagtttc gtacatcgac aacgaagaat
    14101 gaattcgtcg ttcattatca aatcaaacca ttggacgatt cgacctgtga ggttcattac
    14161 acagaaaaaa tggagtctta tggcatgctt caaaagttga atgatatgct tttaggaaca
    14221 atcttgatgt attttaaacg gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg
    14281 tattaaatca atgaaacatg gtactattta gttattgaaa aatgaagtaa gctagaagga
    14341 atttttcata ggaagataga tctttcctgc gaatcctcac tagctttttt gtaggaaggg
    14401 gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc ttcgattgag gaaatgtttg
    14461 atttagccac ttatgcattt aatggggcag atacacctga aaggcgagaa cgttttcgta
    14521 cacttgcgga gaattcttgg aattacggat tttttgatga ggaggatcgg ctaacaagtc
    14581 aagtcatggc gacccctttt ccagtgaatt tttacggaca agagtaccta atggccggca
    14641 ttggctatgt tgcttcttat ccagaagcaa ggggacaagg cgggatcaat cagatcatgg
    14701 aaaagatcct tgaagattgc cgtgatcgta aagtaagctt atcttatctg gcgccattct
    14761 cctatccctt ctatcggcgc tatggctatg aacaatcatt tgataaaatc agttatcaac
    14821 taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt
    14881 ttgaagatgc aaaagctgca atcagaagaa tttatgaaca gatgccggaa aaccaacgag
    14941 ctggtctgaa gcgcgaagac tggtggtata cgtataaatt caagcaaaaa ggcaattacc
    15001 agtttgcttt gcattttgat gataatgagg aagcaacggg atatatcgtt tatcagcttg
    15061 caacgtcttc ctttattatt gcggagtggg gatttttgga tcatgatgct ttttgcagcc
    15121 ttgttcgctt cgtaagttct cacaatggtg cttttgaaac atttgaatat tcatgcggct
    15181 acggcggtaa tgatcaaaat tatctattag aaaatccatt tgcttctgta atgatcacac
    15241 cttatatgat ggcgaggatc gtagatattt ctttgttttt ggaaaattat ccatttaaaa
    15301 agagacaagc tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa tggaatgaag
    15361 gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa
    15421 aactcccaat cgttcgagga agtatccaac atatcacgca gctattatta gggtatcgta
    15481 aagtggaaga gttggtattc caagaaagaa tacaggtgac aaaagaatat gaagaaacgt
    15541 taagctcaat cttgccacag caacagccaa ttcttaatga ctatttttga taaaaaacgg
    15601 gtgatgacaa tatttttgtc accacccttt tgtttttcat ttggcatttg aaggaaaatg
    15661 ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg ttttcttttg ttttagggtt
    15721 ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt ttttagatga
    15781 atctaagcgt tctgcgtata ctttttcttt tggcttatca ttcattggaa aggacctccc
    15841 tacattattt cattctacct ctagtttatc atctgttaag gactagtgga aacgttttgc
    15901 ctaacttata agctgatttt tttagcagat aagcttgcaa gtaaaaaaca aacaagctac
    15961 aattgattta ttgaaattgt tcctgtagct cagtaggata gagcaatcgc ctcctaagcg
    16021 ataggtcgct ggttcaattc cagtcaggaa cgtataaata acccctgtat cctttgcggt
    16081 acaggggttt gttttgtatt tgtgccaaaa atgtgccaaa ctagttatca tttttatata
    16141 aactatcgaa cacagtattg attttttctt tatcctgatc ttcaagctct cgaacaatat
    16201 gaagataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa
    16261 gtatatttac tccttgataa agtaaaatag atgcatgtgt atgccttagt ccatgcaaag
    16321 taaattgctt ttcaatccct aattctttta attttttgcg caaatatttg ctgacggcgt
    16381 ttgatgaaac taatccattt tttaagttga aaaaaacaaa attatgaggg tttttgattt
    16441 caaaattctc gaataattct ttttgattga ttttaaactt ttttaagaga tcaatcgtat
    16501 gtttatcaat ggaaattttc ctatttgaag tttcgttttt tgtttttccc cattcattta
    16561 atttataatt ccatgtttta ttaatagtga tgatttgatc ttcgaaatcg atatcttccc
    16621 atgttaatcc tagaagttca gcaaatcgca ttccagtggc accagcaact aacacgagca
    16681 taggagaaga gtactgcgca gaaaggtttt cttctacgag tttcattaaa gatttaaatt
    16741 gatcataatc taaatattta tcttcttttg gcttcaatga atcttttcct ttgattactg
    16801 cttttctagt tggatcaaac ggaattaatc cttcttctac ggcatctttc aacgacgctc
    16861 taatatggtt attaaactta ataacggtag attttacatg gtctttagca tacttattta
    16921 aaaagcgttg atatcccact ctatctaaat cagaaatcaa taccgctggc atatatttct
    16981 ttatgttcat taacgtatct tcgtattttc tataagtgat aggagatact gttccttctt
    17041 tataaagttg catccaatcc tcaaagtaat ctgataataa tagatttttg cgatcccctt
    17101 taaggccttt gttcaagtta tattctaatt cgttggcggc atcttttgct tctccttttg
    17161 tcttaaatcc tgattttctt atcttcgtgt attttccgtc atcttttttg tatgaaattt
    17221 cgtattgcca actatttcca cgtttgacta atcttgccat aattgaatca gctctctttc
    17281 tctgatacaa taggaactat aaagaagccc attgtttggg ttttattatt ttttgcacat
    17341 cctttttgct tgccggcgca aggatgtgct atttgtcgtt ttaatggtat aattggatgt
    17401 gtattagatc atccaatcag aggtgtttat tatgattaaa gtgattttta aaagaataac
    17461 tgaacttcca gagtttagat tatttgttga ttattggtat gtctggtttt ttattgttgc
    17521 attgctgata ggtctagatg tcatattaga aagaaaactt aagtaagagt tgagttaata
    17581 aattgaattc ctaaatgaga tattttgtag cccatatttt gtttgccgac atcacctaat
    17641 actatggagc taatcgtttt ttctaacaga ccaaaatcag ataatctatt taagctacca
    17701 tctattagct cttgtgaata gccagacatg ctgatatttc cagtgaaatt ttcgtttcca
    17761 cgactcatta agcatgctaa caattgaatc tcaatcgatg ttaattgatt cagtacaaga
    17821 agataatact cctcgttatc ccatgaagcg ctgttaattc gaagtaaaca attcttataa
    17881 agatttttat acatatctat tttattttgt gcctttgatt tttctacctc gtcatttatc
    17941 ccttcgaata ttccaatgag ttggtccttg ttctctaagt tgaaaacatc ttctggaatt
    18001 tgatcgcgaa tgttttctaa tgattcgttt aaatttttgt agaatttctc gatgcgtttg
    18061 aaacgcttct cgtttttaga cccaaaataa agtgactgca caacgccacc aatataagga
    18121 attgctccaa taccgccttc aactattaat tcaagatatt cttttttggt tagtttttca
    18181 ttgctattat ccatagttac ctcctatttt gtcatttcct ttacaagcat tgtgactcct
    18241 gatggcactc ttttgtatag gttttattat tgtgcacaat ctaactctta gtggggcgtg
    18301 gttcatttat tttttactta ctttactatt aatcatatcc cattgagcat aggatttttc
    18361 tatgttcgat cctcttgaac taatttctcc tgtaaaagtt actttatcgc cctttgtaaa
    18421 ggtatcagca ttgtttaagt ccttcgcaaa aattacatat gaaactttag gtgttgcaga
    18481 aatatcattc caacttttat catcgtactc tttgtctgct ataatggcta ttctagtagt
    18541 cattgattcg ataacagttc cttcaaaagt atatgtttta ccactaatat atttagtaaa
    18601 agctgtattt tgctcagtag ctggagtaat agaataataa ctatctaaga aagcacccca
    18661 gtcatgatta tttgtctcta aaaattcttc aaattgagtt aggcttttat tgctgctgtt
    18721 agatgtagta gtttgagtat cagatgatga gctttcctgt ttgttattgt catttgcctc
    18781 gtttgatgag gaaagcgaga aacctacggt agtaaaaata atcccgataa taaggagaat
    18841 cataggtgtt ttcttatttg tttttttgat aaatcttaca attaacatga ttaaacctat
    18901 aataaataaa acaataccag taaaaccaaa tataccgtcc attaatagat cctctttctc
    18961 ttttgatatt gaatattata aaccaagcaa ttcttttttc ttaaaatcga attcttcttg
    19021 attaataatc ccttcatcta acaactcttt atacttttta atttcatctg cacttgaaat
    19081 ttgtgtttct ttggaattat tatctaatat agattgttgc ttctctatat atttttttaa
    19141 attttccatt tcattccaat atttttttgt aaacataacg gtgttttcat ctttcgtggc
    19201 cgctaatata cctccgcgac tttcgtttcc gcctagcaag gtaaattgta tataaccatt
    19261 actcatacct ggttttttta attgtacggc tgatatattt ttaaagggga tagttttttc
    19321 tcctttaata ccttgattaa ctaaatttat aaatcctttt ctaataattg aaatagaatc
    19381 atcagttact ttaatcaagg tttttcctgg agattttatt aaaatctctt tttccatttt
    19441 ttcttcctca tttctgtgat atgatttttt ttgtagaagc tcatatatga ggaaagagcc
    19501 cgtgttgcag cacgggcttt tttaatgttt tgctgaaatc ggttttttta ataattcttg
    19561 atgtattcat acatattgcc ttgcgtaaga atattttttc ttaaaatagc attggcagac
    19621 agcataacaa gtgtatctga gcttattctg aatagaataa tactccataa atttttcgag
    19681 attaaactga gattcatcag tcagttcgtt ctcaatatag atatttaata aaattagaat
    19741 agctatttta tctgcttctg tttcaaattt tgagtgaaaa gttgtagaag tatcgtacaa
    19801 aactgaaaat tcaaaatgag aggcgctgaa atgtgcaagt tcatgagata aatgaaaggc
    19861 ttctgcagtt tcaccgtata gattttcatt caaaaaaatg attctgggtt ttggatagta
    19921 gaaaccaggc tctttcatct ccatataaac tactttcaaa ttgtattcgc tcaacatttc
    19981 tttcaatttc aaatacatac aaaccatcac tccaactatt cattttcctc taaagcttta
    20041 gcaattgcaa tcgctttacg cattgtctcc ttagatattt cttttccatc aaaagaaaaa
    20101 acagtatcgt cttctgataa atccacatgt ttaggggttt ctctttcttc tctacctaga
    20161 aggtagtcta cagagacatc gaaatagtca gcaatccgac ttaattcatc tgaattagga
    20221 gtattatttt tccactttgc tagataaccg ttagaatatc caaaattaat ttctaactgc
    20281 cgtatagata tctttttcct cttcgccaag tcttttatta tttcataggt attcatcgat
    20341 aaaacaacct ttctaaatgt ttacaaaaaa agtttagaaa aatacgcaga aataatttga
    20401 ctaattctga gtaatgagct atactatatc ttgtaaacag atttaacaac taaaaagaca
    20461 acaaaaaaca ctattgatat gtaaatgcag accgccaaga aagctttaaa atcaatgttt
    20521 ttatgtctta tttaattatg ctcttagtat agagtattac tcagatactg tcaattgaat
    20581 ttagaaaaaa gttgttaaat ttgtttacga atataaaaga aaggaatgag aaatatgaac
    20641 acaccgcaaa tttttaattt cgaacaaaac gaagttcgga caattttagt aaatgatgaa
    20701 ccatattttg taggcaaaga cgttgcgagt gttttgggtt attcaaacac taaagatgct
    20761 ttgtcgcggc atgtagattt ggaagataag atggggtcgc gaatcacgac ctcaggtcaa
    20821 tcaagagaga tgacaatcat caacgaatct ggtttgtaca gtttaatctt aaaatcaaaa
    20881 cttccctctg ccaaaaaatt taaacgttgg gtaacaagtg aagtgttgcc agcaattaga
    20941 aaacatggag gttatctaac tccagaaaaa gtagaagaag ctttgcttaa tccagataca
    21001 atcattcaat tagcaactca actaaaagaa gaaagaactg gaagattaat cgcagaacaa
    21061 aagattgcag agtacgaacc taaaatctcc tatttagata gcatattatc ttctacagat
    21121 tcagtaacaa ttagtcagat tgcagcagat tatgggatgt ctccacaaca gatgaataaa
    21181 ttacttcata aactaggtat tcagaaaaaa gtcggtaacc aatggttatt gtgcaaaaaa
    21241 cacatgaacc aaggatacac aaaatctcat acaactgaga tcccgaaagc cgatggtggc
    21301 actaaaattg taatgaatac caaatggaca cagaaagggc gtctatttat ctacgaatta
    21361 ctaaaaaaag aaggatatta ccctcaaatg gatttagagg aaattggtta gaaaggagtt
    21421 ttagtatgac tgacattgca gaaatcactc aacgagatag agaaaaaatc aaagaatatg
    21481 tcgaaagttc aaagttctta acttacacca tgcttgctga aagatttgga attagcaaaa
    21541 gctacttatc tttaatttta aacggtaaaa agacttctgc agaagcaaac agaattatag
    21601 attcgattat cactatgtac gaattgtaaa gggaggaaca gctaatgcaa tatctagaag
    21661 caaaaatccc aattccagaa ggctatgtaa ttatctccca agtggattat gaggagttaa
    21721 aaaaagctga tgatactggt agatggatga cgttgccaga agtgctagaa cggattaaca
    21781 gaaaatatga ttggtttact tctagagttt taaagaaccc aagatataga aatattatcg
    21841 atatcgagaa aaacaaaaat ggatttgtct attatccagt tgaaggaaga gacacatatc
    21901 tatttttaag aagtaaaaca cttgaatttt tagaaacaaa tttttcggaa atcttaagga
    21961 ggcaagcgga tgggaaaatt taatagagcg ctagt
//
LOCUS       DACEPW010000028        21461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEPW010000028 DACEPW010000000
VERSION     DACEPW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.028"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..319
                     /locus_tag="IW819_000848"
     CDS             131..319
                     /locus_tag="IW819_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243970.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            327..521
                     /locus_tag="IW819_000849"
     CDS             327..521
                     /locus_tag="IW819_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243971.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(554..766)
                     /locus_tag="IW819_000850"
     CDS             complement(554..766)
                     /locus_tag="IW819_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243972.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            891..1094
                     /locus_tag="IW819_000851"
     CDS             891..1094
                     /locus_tag="IW819_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243973.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            1091..1363
                     /locus_tag="IW819_000852"
     CDS             1091..1363
                     /locus_tag="IW819_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAQ1243974.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            1364..1549
                     /locus_tag="IW819_000853"
     CDS             1364..1549
                     /locus_tag="IW819_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243975.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            1536..1883
                     /locus_tag="IW819_000854"
     CDS             1536..1883
                     /locus_tag="IW819_000854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243976.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            1843..2319
                     /locus_tag="IW819_000855"
     CDS             1843..2319
                     /locus_tag="IW819_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAQ1243977.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(2465..3193)
                     /locus_tag="IW819_000856"
     CDS             complement(2465..3193)
                     /locus_tag="IW819_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAQ1243978.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            3241..3519
                     /locus_tag="IW819_000857"
     CDS             3241..3519
                     /locus_tag="IW819_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243979.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            3521..3907
                     /locus_tag="IW819_000858"
     CDS             3521..3907
                     /locus_tag="IW819_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243980.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            3904..4284
                     /locus_tag="IW819_000859"
     CDS             3904..4284
                     /locus_tag="IW819_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1243981.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            4396..4848
                     /locus_tag="IW819_000860"
     CDS             4396..4848
                     /locus_tag="IW819_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAQ1243982.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWSDKKMKALDLLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            4845..6572
                     /locus_tag="IW819_000861"
     CDS             4845..6572
                     /locus_tag="IW819_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1243983.1"
                     /translation="MIEPGVNYADLFAKEVRKKPGKYPKTVRLAVDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFILEPWEKFIYSWMYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAATILN
                     NPILLEGTKIFKGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGNLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            6636..7823
                     /locus_tag="IW819_000862"
     CDS             6636..7823
                     /locus_tag="IW819_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1243984.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNDSDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQNVKGGDGNEEEGN
                     SHD"
     gene            7789..8364
                     /locus_tag="IW819_000863"
     CDS             7789..8364
                     /locus_tag="IW819_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAQ1243985.1"
                     /translation="MEMKKKEIRTIDITNFSTRSDEETHTRIISGYAAVFNSPTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLIKSMERGDINQCSFGFIPTEETWDYNSEPMLRTISEVELYEVSIVPLPAYED
                     TEAALRSRDDLEKNIAQRKNLIKKINQALEA"
     gene            8377..9741
                     /locus_tag="IW819_000864"
     CDS             8377..9741
                     /locus_tag="IW819_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1243986.1"
                     /translation="MDKKLLKKLQARHEQRLTELRGRIESGEVREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGKPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            9743..10021
                     /locus_tag="IW819_000865"
     CDS             9743..10021
                     /locus_tag="IW819_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1243987.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            10002..10328
                     /locus_tag="IW819_000866"
     CDS             10002..10328
                     /locus_tag="IW819_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1243988.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDEREMEL
                     SLAYVD"
     gene            10318..10656
                     /locus_tag="IW819_000867"
     CDS             10318..10656
                     /locus_tag="IW819_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243989.1"
                     /translation="MSISFDELKTALKSTKLPVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQISFFTLGTEQDLAVIEEALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCMENAK"
     gene            10646..11011
                     /locus_tag="IW819_000868"
     CDS             10646..11011
                     /locus_tag="IW819_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1243990.1"
                     /translation="MPSNKNGFSEISDYLGNLSRVDPKKLSLESLEEAAKFYLEQLLP
                     NIPKSLLKKKHMSEQIKVVVEEDRVKVQFEETAFYWRFTENGTTKRKAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            11018..11644
                     /locus_tag="IW819_000869"
     CDS             11018..11644
                     /locus_tag="IW819_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1243991.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVVRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIAVMDEIKGMIAKSGVTFSKN
                     TAREFPYLAFGFIGNIEGGGKKAVWYPNTQLSNVIDEEYATAEDETKIDDVTANFVST
                     GLKYNNVMYASFDSNREEATMDLFEKFIAQPVYDEEQWKTLAKVGGAG"
     gene            11644..12108
                     /locus_tag="IW819_000870"
     CDS             11644..12108
                     /locus_tag="IW819_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243992.1"
                     /translation="MARLSDYGINVEDLKNSATVTIQGTEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            12303..14612
                     /locus_tag="IW819_000871"
     CDS             12303..14612
                     /locus_tag="IW819_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1243993.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            14624..15328
                     /locus_tag="IW819_000872"
     CDS             14624..15328
                     /locus_tag="IW819_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1243994.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            15325..18084
                     /locus_tag="IW819_000873"
     CDS             15325..18084
                     /locus_tag="IW819_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1243995.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            18097..19005
                     /locus_tag="IW819_000874"
     CDS             18097..19005
                     /locus_tag="IW819_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ1243996.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            19005..19622
                     /locus_tag="IW819_000875"
     CDS             19005..19622
                     /locus_tag="IW819_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243997.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            19626..20075
                     /locus_tag="IW819_000876"
     CDS             19626..20075
                     /locus_tag="IW819_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243998.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            20075..20569
                     /locus_tag="IW819_000877"
     CDS             20075..20569
                     /locus_tag="IW819_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1243999.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            20583..21014
                     /locus_tag="IW819_000878"
     CDS             20583..21014
                     /locus_tag="IW819_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244000.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            21016..21153
                     /locus_tag="IW819_000879"
     CDS             21016..21153
                     /locus_tag="IW819_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1244001.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            21190..>21461
                     /locus_tag="IW819_000880"
     CDS             21190..>21461
                     /locus_tag="IW819_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244002.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT         7543 a         3304 c         4579 g         6035 t
ORIGIN      
        1 aaattgctgc agaccatgaa ttatcagaaa ctgctttaat tgatcaaatc gaattagcta
       61 gacaatactt tgttccatat gcagatacct ggtccaagca taagaatgac atcatttttc
      121 cagaggaaca atgagagcaa ctgatccagt aattatcctt gaggaagcca aatttatttg
      181 gactcacgaa gagatagagc aagcacgctt gctcttttct caaggagtta agccgagcaa
      241 agtagctgaa ataatgggtc aaaagattct tgatgtcgga ttgcttttgc tccatctagc
      301 agaaaaaaat ttgatttgag gtgaaaatga tgattcaact tgcaggcatc caaacaggga
      361 aaatttattt ttctggagaa agcaaaagcg aagccaacca atggctattg aaaacttaca
      421 cgaacaataa gaagctaagg aaaaaatatc ctgatttgtt gctaaaggat gatcagatta
      481 tgccggaacc gatgattttg acccgtaagg agggaagttg aaactaaaaa accagccgtg
      541 tgggctggag ttgctatttc tttttaataa tgagtttttc taaatttaat tcattctcac
      601 gagtttgcgt ccaataatca gaataatatt ttaattgttt aagagtagga atgtcgtctt
      661 cggtaaaatt agaaaataat tctggagaat tatctttttt taattgttct aatcctgatt
      721 ctttaaaacg ttgttcaacg agttttacta aattgttgat attcatgata aatcaccttt
      781 ttaaatttaa tttcgatttg attaatcgat acaagtatta tatcaaataa aaaagctgat
      841 ttagggggtc ttacaaaaac actaaaattt tttcaaaaag aggaggaacg gtggtgaaag
      901 tcagagaaca attcaagata gtgagcgacg aagataatgt ttacaaaata aagatcgtta
      961 atattaatga atatcgagaa gcagatcaca aatatttggt tgacttatac atgaacgata
     1021 aatatttggg agaaggattt tgtggagatt cactttttga tgatgagcga attttgaagg
     1081 aggaaacaga atgatccacg aactaaaaat actaccagaa tattttgaag cagtgacgag
     1141 cggacgcaaa caattcgaga tccgcaagaa tgatcgtgac ttcaaagttg gtgatcagtt
     1201 aatactacgt gaatataaaa aatacattaa acatgaatta cctggactaa cgagaccaac
     1261 atttagttac actggtgact catacaaagc agagatcact tatattactg attacatgca
     1321 gaagtccgga tatgtagtgt tggggattaa aggggtgaat taaatgaaag tatacatctt
     1381 agctatcaca gagggcacat ggatgttccc agtcggttcc ggaaaaatct ataaatcaaa
     1441 aacagctgct tataaagcat ttgaaaaata caaaaaagaa aacggtggcg gcactaacgc
     1501 aaagattcta gtggctgata actggcatga agaaggtgaa cgtaattgat ttacctgttc
     1561 attttcgtgg tactaactat ttttatgcta gtaatcttat tcaaaaaaag atgagtaaag
     1621 acgaacgaaa aattctggcc aacttttatt tagtattaaa aatcgaacaa aatgtgtttt
     1681 cggaaaggag aattgtatgg gaaaaacaaa atcaaagatt aagaagaaaa aacgacgttt
     1741 ggaacaaaag gcaatccaga atggaacggc taagaaaaaa taaaaaagct acccctttcg
     1801 agatagcagc gcaatactat tctaccataa ggggtggcgt ttgtgagatt tcattggtta
     1861 aaagattacc aagaactaga tgagcagatt ctttacttaa agtggaatct taataaaagt
     1921 aagcttgaat tgaatcgatg ggtctgtggt gatttagcaa acgtccgcat cgaaaagaat
     1981 tcgagaactt cttctttaga agagaatatc caaaagatag aaaatgaatt ggaattgttg
     2041 atcgagcaaa aagaagaaat gttattgctg atagatagct tttctggtat cgataatcaa
     2101 attgttaaga tgaaatatgt tgatcaaatg agcttagaag atattgctga atctgttggg
     2161 tatagctcat cgtatgtcag acagcgacat gcagaaatta gaaagacatt gaacttttta
     2221 gatgagtatg aacacagaca agctgaccga ttgaaaaaag aaaacgaaat tgatttttac
     2281 aatagcgaga agtacaaaga acaactctct ttattctaaa attacactgt tcactaaatg
     2341 ttcggtcatt gtagctatgt aaacattgtt agcagcatga tattctatta gtgtcaaaaa
     2401 aatatgaaag agccaagata tcccaaccgt tttattaatt ggtatctgtg gctcttttat
     2461 tcttttaatt agtagttggc ataaagttat ttccatttat aaattgatca gctaaatcag
     2521 catcataatc agccgcgata aattgcgctg ctgatttaat aaatgcatcc atatcatgaa
     2581 catctttatt agtatgtcta cgaacatagt gcgtttcatc attaccaatc caagtagcag
     2641 ctttggctaa tctttggagt ttaggaaaat cattcaaata attttcaatt gttttaccta
     2701 ataacatttt ttttatttta tcttcatcct ctgaattttt agatattgca taatctttaa
     2761 ttagaaattc tagagctttt ctataaccaa ctccagcaat ttgttccaat tgctcagatt
     2821 tagcttgtgt ggcttgagta taaatatcaa caaatgtagg agaaactttt tcaatatttt
     2881 ctggtaaatc tattttaatc ggcggacggt aataatcttt aattaaaata ttttcagaat
     2941 atccttcatc ttcattaaac gtatttataa attgtaaact ataaaatttg ttgcactcat
     3001 catttataca ctgtactaat acgccaaaga ttctatcatt atcgaacgca ctatatgatg
     3061 aaaccccaaa atacacatac ggagtcatta tatttccgca atgcggacaa ctatttggca
     3121 aataaattgt ctctgtattt tgacgatcac gatacttaat attaacttct tgaagcatta
     3181 aaacacctcg catctttttt ataaatcata ccacaaaata tctgatagga gagtaaacaa
     3241 atgaatgatt ttcatgaagc ggtacttagt attgaagtag aatcaagttt ggctaaagtt
     3301 tataagaaag caattgaagc agagaatagt ccgtacagag aaaattggaa tggtaatcat
     3361 gcacatgtgc aagtggagaa tgatgattac agaactggaa tgaatacgtt agttatttct
     3421 ttattgtcac acactctacc caatctgcag gaaacgattg aatggtatga gcgaatgggc
     3481 gcaaaagtaa tccgtacaaa ttacaaagga gagaattaaa atggcaatga tcaaaattag
     3541 aacatccatt agtggaacag agtattggga ttcagaaaag aaaaggacag tagttgttcc
     3601 gaaagatcaa gaaccaaatt ttgaagtaaa agaagaaaaa ggaattttga gtgatggcaa
     3661 atcatttgtt gctgtcaatg gcgaactcat agctcatagc agtgaaattc ttgatagcga
     3721 tggaaacact gctgctaatt ttgatggaga cgaagctact aatgatcagt ccactgaaga
     3781 tacggatgaa ctggacaata tgactgcaaa agaattgcgt gcatatgcta agaaacatgg
     3841 tattgatatt cctggtgcta tccgtgcaaa aggcgatatc ataaatctta ttcgtgaagt
     3901 agaatgaaat actgtcagtt tgacggatgc acgaacaaaa tagcaaaggg tatctactgt
     3961 actgaacaca agagatcaag cagatcacgc aagaagaagc agcaagcaaa gtctgtttat
     4021 catcatgaga acaaaccatt ctatcgaacg caagcatgga aagatatgcg tcaatttatt
     4081 tatgaaagag aaggtggtca ttgtcagcgg tgtggtcagt tcatatttgg aaagagagca
     4141 cacgtccatc acattgtacc aatcaaagac aacgaactgc ttaagcttga tccaaacaat
     4201 ctcatgctat tatgttcaaa atgtcatcca attgttgaaa acgaaacgga agacaaaaaa
     4261 gtttttcctt cgtatttcaa ttgaagcccc cctattcatt ttcaaaattt tttcgcgtgg
     4321 ggagataggg tagcggggag tcacgcgcat cgttaggtca aatttttcaa aaaacaaagg
     4381 ggggtgtata caagtatgac gactaaagcg caacgtaaag cgattgttga tgaaaaagta
     4441 agtgctgaaa aagctcgtat tttaaaaata atgaatttgt ctgatttgta caccatcact
     4501 cttgatccat taatcgaatc atatttggat atttttgaaa tttaccaaca caaatatctt
     4561 ttgtggaagg aaaaaggctt tccggagacg caaaaattca cgaataaatc aggtgccact
     4621 aatcaatcga agcatccatt ggcgcagcaa gtagaaactt ggtcagataa gaaaatgaaa
     4681 gctctagatt tgttggggct aacgaataag gctaaaactg gtagacaaat aactggcgga
     4741 tcgactgcaa gaaaagatga agaaattaca cgtccagaag aaaagccagt agatgaacta
     4801 gcagcgcaca gaaacaaatg gcgtaagaag gcaggtactg aaaaatgatc gaacctggtg
     4861 taaattatgc cgatttattt gcaaaagaag taagaaagaa acctgggaaa tatcctaaaa
     4921 ctgttcgttt ggcagtggat cgttggtatc ggtggaaaaa acgtaaagat atttggtttg
     4981 atgtagatcg tgcgaatgaa atgatggact gggttgagtc gtttattgtt cacacaaaag
     5041 gtgagatggt tggaaagcca ttcattttag aaccttggga aaaatttatt tactcgtgga
     5101 tgtatggatg ggttaaagaa aatgaaaaag ggcaaattgt ccgtgttact cgtgaagcgt
     5161 atgtccaaat ccctaaaaag aacggtaaaa cattgattgc tgtcggttca ttaggctatg
     5221 caatgtatgg agaaggtgct ttgtcagtcg attgttatgc atgcgcttct gactttgctc
     5281 aagctcaata tgctgccaaa ccttttgcag ctacaatatt aaacaatcca atcctacttg
     5341 aaggaactaa aatattcaaa ggaccaaagg gaactgtttc tagtattact tatgattata
     5401 ttcatgaaga tatggcttat tcgaataaat ttattgttca aacgaagaat atcgataaca
     5461 ttgagggttc taatccatat tttgtattga atgatgagct gcataagcaa gagaaaatgg
     5521 aacagtatga caattttaag tctgcacaaa tctctttgcc acaaccattg atgtttaata
     5581 tttcaactgc tgggaaaggt tcatcgtctg ttggaatgcg cgtatatcgt gaagcaaaag
     5641 aagtgttgaa acgtgatgat aatgattcga actttgttct aatctatgaa ccaaataaaa
     5701 actatgattg gacggataga aaagtttggg aaatgtgcaa tcctaactgg ggaatatcag
     5761 ttgatctttc cgccttggag tcagcgttta aaacggcaca acgttcagca cattcaaaag
     5821 ccgagtttct aacgaagcac ttggatgtat ttgtaaacgg tgcggataat ttctttgaac
     5881 aggatcaagt agagccgtgt ttggttacta cccaagagct tggaaattta agtggtgaac
     5941 cttgttacat tggtttggat ttatcacgca cacgagattt gacctgtgtg tctttaaact
     6001 tcccaacgtg ggatgaagat ggaaaagcgg tccttaaagt gaagcagctt tattttattc
     6061 ccaatgaaga tttagagttt cgagaaaaag aagataatgt tccgtatagt gatttagctg
     6121 agcaagggtt tgttgaattt tgcgatggca aaatgattga tcaagatcaa atattgcagt
     6181 atatcgaaga ctgcatggat ttatacgata tacaacaagt gaattatgat ccagcgatga
     6241 gcgacaaact tgttgagaaa ttggagaact taggattgga atgcgttgag gttgcccaat
     6301 atcctaaagt gttgaacgct ccttttgatg atgtcgaacg gttgttttat gagaaacgaa
     6361 ttcaatttga taatccattg ttcctttatt gcaccttgaa cgttgtagca atcactaaca
     6421 tcaacggaca aaaagcgcca agtaaacgtc aatcaaagaa aaagattgat ggtttcgtgg
     6481 cgtttttgtg tggtcataag gaaacgatga atcagatgac agatattgat tcggatgaat
     6541 tagatgatta ccttagttcc atctatagat aaatagaaag gcggtgagaa atattgaaat
     6601 tacgtgatcg gttatcgaat gcagtctatt cctttatgga aaagcgtggg tacatcgagg
     6661 atatgtttgg ccactatacg cgttacggtc aaagatatgt aacagattcc tctatcatgg
     6721 aatcttctga tgtttatgaa ttggtccagg atatctcaaa ccaggtagcg ttgtcctcac
     6781 ctgttgtcat aggtccagac ggtaatgaag tgaaggacca ccacttgcta aaaattttga
     6841 agaatcccaa tgattatttg accggattcg aatttaccaa attggaaaca aataccctac
     6901 tgatcaatgg tgagacattc ccattaacag atcgggacca gcttcatttg gcatacggtg
     6961 taaccactaa aatcaatgaa cgacttcaag aagaatttga aatgaatgga caaaaaatac
     7021 ctggtcaaat gattcgacat attaagaaca tcggaactga ttcattaaaa ggtgctggaa
     7081 taattgacct tgcaagaaac actctggaag gcgttctgag cgctgaaaaa gttttgacgg
     7141 acaaatatac taaaggtggt ttactcgcgt tcatgcttaa actggacgcc cacatcaatc
     7201 caaataatag cgcccaaacg aaaattgtca aagctatatt ggatcaactg gaaggaacgc
     7261 aaaatgacag tgatcattct gttaagatga ttcctctggg aaaaggatat tccatcgaga
     7321 cgttaaaaag tcctgttgat gacgcggcta ttttgaacta tttaggtgtt tataaaaaag
     7381 acttaggtaa atttttagga atcaatgttg atacgtatca atcgttgatg aaaacagaca
     7441 ttgaaaaagc gatgatgtat ctgcataaca aagcaatcaa accaatattg aagaacaaga
     7501 gcgaacatta caccgctctt ttttttatgc caaattctgg ctatcgggtg gaatggaaaa
     7561 tcaacatctt ggattttgtt ccttactcaa caaaaacaaa tattggctac aacatcgtac
     7621 gtactgggat tacaagtcca gataatgtgg cagaaatgct tggtttccct aaacagaaca
     7681 ctccagaaac acaagctatc tatatttcaa atgacttatc taggattggc cagaaaaatg
     7741 caacagatga ttccttacca acgaatgatc aaaacgtgaa agggggtgat ggaaatgaag
     7801 aagaaggaaa ttcgcacgat tgacatcacc aatttttcaa cacgttcaga tgaagagact
     7861 catactagga taattagcgg ctacgctgct gtattcaaca gtccaacact tttatgggat
     7921 gacttaagcg aagtgattgt tcctggtgca ttctcaaagg caattagtaa ttcagatgtt
     7981 cgttgtttgt tcaatcatga ttggtccaat gtgctaggac gtactaaaag cgggaccctt
     8041 cgacttgaag aagatgaacg tggattgaaa ttcgaagttg atttaccaga cacaacggta
     8101 gcaagggatt taatcaaatc gatggaacgt ggcgatatca atcaatgtag ttttggattt
     8161 ataccaactg aagaaacatg ggattacaat tcagaaccta tgcttcgaac aattagcgaa
     8221 gtggaattat atgaagtttc tattgttcct ttgccggctt atgaagatac agaggcagcg
     8281 ctaaggagcc gtgatgattt agaaaaaaat attgcacaaa gaaaaaattt aataaaaaaa
     8341 atcaatcaag cgctagaagc gtaggaggaa actattatgg acaaaaaatt attgaaaaaa
     8401 ttacaagctc gtcatgagca acgcttaacg gaattacgcg gacgaatcga atctggtgaa
     8461 gtacgtgaag cagatttaga agccgtgaaa gaagaaattg acagtgttat tgatgaattg
     8521 aacggaatta aagacgaatt aggcgcagat tctggaactg atgaaacaga cgacaataca
     8581 gatgatcaat cgaatagtac ggattcagac gaaagccgtt ctggtgaaga caacgatcaa
     8641 gaagaagatt cagacagtga agatagttca gaaaatcgtt ctggaatgat tactcaacag
     8701 caacgagatg gattacttgg atcaattaag aacggattgg aggcacgtgc aaaaatgacc
     8761 aataaacaaa aagatcaaca actacgaaaa gcatttgcta attttgtagt tggaaatatt
     8821 tctgaagcag aagctcgagc tttagggatt gaagctggca acggttcagt tactgtccca
     8881 gaagtaattg catctgaagt tattacttat gctcaagaag aaaatttact tcgtaaatac
     8941 ggaacagtgg tgcgaacatc aggagatgtc aaatatccaa ttcttgtgaa gaaagcagat
     9001 gcgaatgtaa acaagaaaga gcgttcaact gatattgctg aaacagctat tcaatttgat
     9061 gaaattttgc ttgatcctgc cgaattcgat gctttggcaa cagtaactaa aaaattacta
     9121 aaaatgtctg gtgttccagt tgaagatatt gttgtggaag aattgaaaaa agcttatgtg
     9181 cgtaaagaaa tcaattatat gttcaatggt gatgacgctg gaaatgaaaa tcctggtgca
     9241 ttagccaaaa aggctgtagc atttgaaaaa cctttagatc taactgctgc aggtgctggg
     9301 caaaaattat atgatgcatt aatcgaattt aaaaatacac cagtgacaga agtgatgaaa
     9361 aagggacgct ttattattaa tcgagctgct ttgactgcta ttgaaaaaat gaaaacagat
     9421 gatggatttc ctttgttgcg tccatttaca caagcagaag gtggaatagg ttaccaatta
     9481 gttggctatc ctgtggattg gacagatgca gcagataaaa agggtaaacc agacacacca
     9541 gttttatatt ttggcgattt ttctgcattt aaaattcaag aagttattgg tgccttggaa
     9601 attcaaaaac ttgttgaaaa attctctggt aaaaatcaaa ttggattcca aatttacaac
     9661 ttgctagatg gtcaattggt ttattctcca tttgaaccgg cagtatatcg ctacgaaatt
     9721 acaaaaccag ttggtggtta agatggaaga gcaaactaaa gaattgtctt tagaggaaaa
     9781 attcaaatca catattcatt ttgaagaggg catggatgat tctttgctct ctttttattt
     9841 aaatatggca aaagattatg tcaaaacagc aactggcggt caacaagaat atcttatttt
     9901 gatggttgcc ggcattgcct atgaatatag agtttcagag gatgaactcg acaaagctat
     9961 gaatgccatg acgccattta tcgtgcaagg agcgattcaa aatgccgaag agacagacta
    10021 ataatctcag atggaaagcc gaattgctag acatcaaaac aggaacagac ggaaacgatc
    10081 gtccaactac aatttacgaa tttaagcgtc taatattcta tgaagaactc ggtgtgactt
    10141 ctcaagaaaa atatttatca cagcaagcca agacagacgt tgtcagacga attaaagtca
    10201 gatgggataa atccatcaca gagaaattaa gtgcgctcaa aattgattct gtaacgtata
    10261 acattactcg catttatacg aatcccgatg aaagagaaat ggagttgagt ttagcttatg
    10321 tcgattagct ttgatgaatt gaaaacagcg ctgaaatcaa caaagttgcc agtgttcaga
    10381 gataaagcca gattagggac gatgtatcca tacatcgtgt actcaaatgt gagtaacagt
    10441 aaaaaaatgg catccggtaa agtctataaa aaattaccgt attatcaaat ttcttttttc
    10501 acacttggaa cagaacagga tttagctgtt attgaagaag cgttacaaaa tgctggtatt
    10561 ccatattcag attttacagg tatacaaggt gatgagaacg acgataccgt aaccaactat
    10621 tacacatatg tgaggtgtat ggaaaatgcc aagtaataag aatggttttt ctgaaatatc
    10681 ggattattta gggaatcttt ctagagttga tccaaaaaaa ttatcattgg aatctttaga
    10741 agaagctgca aagttttacc tagagcagtt gcttcctaat attcctaagt ccctgcttaa
    10801 aaagaagcat atgagcgaac aaataaaagt tgttgttgaa gaagaccgag tgaaagtcca
    10861 gtttgaagag actgcttttt actggcgctt taccgaaaat ggtacgacaa aacgaaaagc
    10921 acaacacttt gcaagcggta cgtatgaaca aaataaagaa aaaatcgaag agatcatgac
    10981 gaagaaaata cttgatttat gggaaggatg attttaattg ggaaagcaag atacttttta
    11041 ttttgaagga ttggacgaca ttttaatcgc gatgatggca acgccagatt ctgttggaac
    11101 tgctccgact tatagtgaag ttgtccggtt gcctatcgct acaaaaattg gagtcaaagg
    11161 caatggtaca gcgttggaaa aatgggcatc aagcaaaatg ttccgacgcg tgtcccgaga
    11221 aacaaaacat gaaattgcgt tggatcatgt aggtattcca attgcggtaa tggatgaaat
    11281 caaagggatg attgcaaaaa gcggagtaac atttagtaaa aataccgcac gagaatttcc
    11341 ttaccttgca tttggattca ttggaaatat tgaaggcgga ggtaaaaaag ctgtatggta
    11401 tccaaacacg caactatcta atgtaattga tgaagaatac gctactgctg aagatgaaac
    11461 aaaaatcgat gatgtaactg caaactttgt ttctacagga ctgaaataca ataatgtgat
    11521 gtatgcaagt tttgattcga atcgtgaaga agcgacaatg gatttatttg aaaaattcat
    11581 tgcacaacct gtatacgatg aagaacagtg gaaaacattg gctaaagttg gaggtgcagg
    11641 ctaatggctc ggttatctga ttatggaatt aacgttgaag acttaaaaaa ttctgctact
    11701 gtcactattc aaggcacaga attccctatc tcatttacta tgcaaacgat ggaatttata
    11761 gcagatgtat atggtggaga ttattcgcaa ttcgaatctg atatgaacgc tatgttatct
    11821 aaaaaagaag gacaaatttc ttctgctaac ttatcgccta gtgatctaaa aatcatgcgt
    11881 gccttgattt atgccatgct gcgtactggt ggtttagagg aagatccaga aactattttc
    11941 aaatttttgg gaatgagtgg agaggtgttg tctgcttata gtacctgtat ggaaattttt
    12001 gctagccaga catttcaggt ggaagaccta aaaaaatcca agaagccaca agactttcaa
    12061 aaaacgcaag caaaaagaaa ggtaaacaaa aagaatcgga agagatagga actccttgga
    12121 gtttttatat ttacgttgct ctcactctat tgaattggag tgagagtttc tttttgaagt
    12181 ctacacctaa cttgtggctc aaatcttatt tacagtggtt acaacaaaac accgattttg
    12241 aaccacctca atctgtaact atggataaat cgccttggtg gtagaaagga gcgctaagat
    12301 aaatggctgg taaagaatct gatgtcgttc ttaattttaa aacgaatggc gaagtcagtt
    12361 attcgaaaac aatcaaagaa atcaacaaag aaatgaactt agccgctgcc gagtacaaaa
    12421 accaagtgtc tgcgatggac aaggatgcaa ctcaaacaga aaaattgcga gcggctaaac
    12481 aaaaattaga aaaacaatta ggattggctg aacaacgatc tcaaatgctg agagaggaat
    12541 atgaaaaatc tgtcaaagaa accggtgaat attctgctga atcagaaaaa ctttataaac
    12601 aattactcaa ttctgaaacc ggagaaaata aacttcgtac agcattagaa cagacgaacg
    12661 atgcacttaa agaacaaggt gacgtttctg ttgatacagc aaaaaaactc cagaaaatcg
    12721 aagaaacagg tgagaaagta aaaggcgttg gtgaaaaaat gtctgttgga gtaaccgcgc
    12781 ctattgtagc ggcaggagca gcaggacttg cagcatttgg tgaagttgac gaggcacttg
    12841 ataccatcat tacaaaaacc ggagcaacag gtgatcaagc tgatagactt tcacagtctt
    12901 tcaaaaacgt tggttcaaat actcatttac ctttacaaac ggttggggaa gctattggtg
    12961 aggtaaatac acaatttgga ttcatggata aaaaactgga agattcaacc aattatctcc
    13021 tacagtacgc tgaaatcaat gatacagatg tttcgcaatc agcaatatct gctcgacaag
    13081 ctattgatgc ttatggacta gaatatgatg atttgaattc tgtccttgat gtaacaacga
    13141 aaacatcgca gaatactggt caatctgtag acgacttgat gcaaaaagca attgatggcg
    13201 caccacaaat taaacaatta gggttgagct ttggtgaagg ggtcacttta cttggacaat
    13261 ttgagcaatc tggtgttgat tcaagcgcag ctttaagtag tttatctaaa gcaacagtgg
    13321 cttatgcgaa agacggaaaa tctttaagtc aaggactaga agaactacaa gacaaggtaa
    13381 aaaatgcagg ttctgaaaca gaagccatca atgcagcagc agaagtattc gggactaaag
    13441 gcggtcctag aatggccgat gcaattcgta gaggtacttt gaacctggaa gatttagcaa
    13501 aaactgctgg agaaagtggg ggagctgtag gagacacatt cgatgctact ctcgacccaa
    13561 ttgatcaagc cgatcaagct atgaacaatg caaaattagc aatggctgac gtaggtgaag
    13621 cagtccaagt aagccttttg cctttttttg aaaaagcaac ttccttatta caggaattct
    13681 ctaaatggtg ggggtctcta gatcaagata caaagaattg gatcataaca attgctggta
    13741 tagcggcagc agttggacca gtactaatcg ttcttggttc acttatggga tctgttacaa
    13801 aaatcgttgg ggaagttaaa agttttatag gagtttggca aggacttgct ggtttatttg
    13861 gaatgtcggg aggctggttt gcattagcag ttatagctat tggcgcatta gttactggac
    13921 taatttgggc atataataac gttaaatggt ttcatgatgg agtgaattct ttcttcaaag
    13981 gtgtttcaga tgtagctgtt gaaatattta attttttaag tggttatttt ggtggatttt
    14041 tcgatggtgt tatagcaaat ttcaataatt tttataatgc tggaaaacga attttcagtg
    14101 gttttctaga ttttatcact ggagtattta cagggaattg gtcaaaagca tggcaaggtc
    14161 tagttgatat ttttggaggt attttcgatg gtattgttgc tgtagggaaa gctcctatta
    14221 atgccatgat tggattgatc aatggattta ttggtggatt gaataatata aagataccaa
    14281 aatgggttcc tggtgtaggt ggaaaatcat tttctatttc aaaactgcct tatttagcac
    14341 aaggcggtca tctaatcaat ggacaagcga ttgttggtga agctggacct gagttactga
    14401 ctgccaagaa tggtaaaacc acagttactc cattatcaga cgaagaaaaa cgcaaaggga
    14461 tcagtggaag agtttctgga ggtagcattg aacagcatat acacattggc aaagtcgatg
    14521 caaacaatcc atctgagtta gatcgtatga atcgtaaatt tgctaaagca aacaaacagg
    14581 ctattcgtga tttgggaggt gttcctattt gagtcgacaa tttatgaatc cagatgaacc
    14641 aaattttatt tggaaaaatc ggaatgcagt aattgatatg aattgcatca ttgaggcaga
    14701 gcttccagat attatgccga acaagcgata cgaaacttac acgattcagg gccgaagcgg
    14761 agaattgacg gagacgtttg atgactatga accttttgat ttggaaatag aagggatcac
    14821 tattccacat tcgaaactga gagaagtcaa acgatggctc actggtaaaa gccaattaat
    14881 cacacataat gatccagaca agtatctaga ggctatctgc agcatggata aagaagtccc
    14941 tttcgaaaac gagtggggct tcttttatac attcgatgtt acttttcgtt gtcagccact
    15001 caaaagaaaa atcggtgaag cacctaaaat ttttcattct agtgttatgg atatttttga
    15061 tcatggtgac gaaattgcac atccctatct ggaaatagaa tcgaacggag gagatataac
    15121 gatcgctgtc ggtgataaaa gcctaacaat acttaatacg ttagctgcaa caatcgtggt
    15181 tgatactcag cttggaaaag caatacaaga aggtttgaat ttatttacgc gtggtgattg
    15241 gccagtattg cagcctgaat ggaatcaggt aaaaatatcc ggaaaattta aagaagtgcg
    15301 gttttggaat aggagtgtgt atctgtgacg caagaattta tttatgctta taaaaaaatg
    15361 ccagatgatt tgagcgtgaa cggagcgtcg ttagttgatt gggaagattt gccagaaatc
    15421 aatcgtgtgc tgaatggcca atatcgtttt tatggtaact attcaagaag cggacagtat
    15481 cgctcttatt taaaaaaagg aaattttata aaagcgaagg taccagatgg atcatggcaa
    15541 tattttgaaa tttataatgt caaaaagaat ttgacctccg tttctgtaac agctcggcat
    15601 attggtttta tggccaataa gaactttatt gtcaagtcgt ttacggataa cggcaatggg
    15661 tctcagatca tgaccaatct taaaaacagt ttggcatttg atcaaaaatt taactatctt
    15721 tcgaatgtag ggacaacgca ccaatttaca gcaagacaag tcgcgccagt tgaagcaatc
    15781 attggttcaa ataacggtaa tcaaaatttg actggcgtga ctagcgcgga acttgatatg
    15841 gataactatg atttgaaact ggtaaaacag attggatcag ataatggttt tcggattgat
    15901 tttggcatca atttagaagc aatcgaagaa gaaattgatg aagaatcaat tgtcaatagt
    15961 ttgtatttgg ttggcggtgt tcctgataac gattatgatg aagacaaaga acctatcgaa
    16021 tatggttatt tagaaattga tggagtaacc aacgagaatc gacgtattgc caaacgtgaa
    16081 aacggagatt gtaaaacagt tgatgaattg atcaaatggg gcaaaacctt atttgacaat
    16141 gatcgtatcc atgagccaaa agctactcat acagtcagta tggtggcgtt ggaacacaca
    16201 ctcgaatacg gtgacatgta tcaagagcta gcgtctttac actttggtga tgtcgctcat
    16261 gttagggcga aagagctaga tatcgagata aaagagcgta tggtcgaata cacttatttc
    16321 ccaacgcttg ggaaatataa ggatattgtt ttaggtaatg atttatcact gtacacttcg
    16381 gcagtaaata ctcaagcaca agaattgaaa aagaaaatcg acaatcgaac agaaacgtta
    16441 gttcaaaatg tgttgaatgc gacggcatgg atcactggta attctggtgg gcatgtcgtt
    16501 tttcgtccag aaaaagctcc atcagagatt cttatcatgg atactccaaa tgtggcgaat
    16561 gcaaagcgag tgtggcgttg gaatttaaat ggcttaggtt attcagacaa cggagtaaat
    16621 ggcccgttcg gtattgctat cacatcaaaa ggagaaattg ttgctgactt catcaaagta
    16681 ggaacaataa atgcagaagt attcgaaact tcctttaatg cttatggtga tgtgttaaaa
    16741 cttgtaaaag gcacgcttca aatttggaat gaaaacaaaa aaatcatgga gctaaccaaa
    16801 aaaggtatgg aattctggaa tagaaaagaa tccattggaa cgatcggaac aacagattca
    16861 gcaggaaatc catttccaaa tgctgtaaca cctacacctc ttgaggaaaa ttctttggta
    16921 atacgtacaa atggcgatgg aaaatacatt ttaatttctc caactgcaga aaaaggattt
    16981 gtgttattag gaaacggaaa agcatattat ttcggtgatt tagatattca gggaaaactt
    17041 actgttagag gtaaagaagt gataccagga caaaatggcg gtccatctgg cggaggagaa
    17101 acacctggtg ggtatcctga tgaattaaaa acagatgcag aaaagagagc ttggagaata
    17161 tacgatattt tgtgtaataa cggatttacg aaacaatctg catgtggaat attggggaac
    17221 attcaacaag aaacaggagg aacttttgat cctgatactg ttcaaatagg tggaccagca
    17281 tacggattag ttcagtggga tggttcttca tatcctcttg ttggtccagc aacatgggat
    17341 ggaaaagttt atgttcaaaa cttatttaat gctgcaggta ttaaagaacc aataacgagc
    17401 ttagatgcac aagttcgatt gcttatttgg acatttacaa atggacaatg gatgggcgta
    17461 gtacaaccta cgacggttga tggatttaag gcctgtactg atccaagaca agcagcatat
    17521 gcttttgaac gaaactatga acgtccggca gcgacacatc ctgaacgtca agattatgca
    17581 gttaactggt ataacaaatt taaagattta aaaccaggag gagctactgg agaagcagga
    17641 ctaaaacatt tagagtcttt gattggacaa cgaattggca atggtcagtg ttatggcttg
    17701 tctgcagaat attcaggata tctgggtggt tgtggcatgg gtgctggaac aaaatatggt
    17761 ttaactcatg tgataggaaa tacttctgca gcatctgata ttggtattgc ctatgattgg
    17821 tctgctgttg gttggaaagt gattcaaaac cctagatatg atcaattagt agttggtgca
    17881 attattaatt gggcaagagg tggacaagtg ggttcatggt ttgcagacgg aacttatgga
    17941 catactggcg ttatccgagg cttagctaat ggtcgtatgc aaacttatga acaaaacaca
    18001 gagttaggta tgatttgtgg aaagttagat cgccagtatt atagtgcaag tgcaatttct
    18061 tccattgtca taccaccaaa atagggaggt gatttaatgg caaagtggaa tgtcatatta
    18121 agtacaacag aaccctataa ttacgtgggg atgattcaag ttcgacaagg caataaaaac
    18181 actgaagtta tggaagctac aattgtcgaa aatggtcttc catatgattt atcagaatgt
    18241 aaggtatatt ttgaatcagt tgttggcgga aaatatccag tccaattaga aacaagaatt
    18301 gtggatgcta aaaaagggaa aatcaaatat atttttgata aatattccat gcagtgtttg
    18361 catcgacaaa cagccaattt cattatattt aaaggagaag acttgattgg aacaactcaa
    18421 gacttctcct attttgtcat caatgctgtt tcaaaaacag aaggagaaat gggttcttat
    18481 tggcaatcaa ttgaagattt aattgctgat atgacagatt ttattaatga aaataaaggc
    18541 gattttacgg actggatgaa tgaaaggaaa gaagaatttg atcgctggag agaagaacag
    18601 gagaatagtt tccaagattg gagagaagga caagaatcag attatttgtc atggtttgaa
    18661 tcaatcaagg atattttgaa gtctatcgat ccaggtggag taatgttagc cgaattaatg
    18721 gatgcacgtg tagatcttca aggtgtacgc catgaatcga tttctgaacg tttcctatcc
    18781 gatctaaatt atttgtatca aaaaatgaaa gcagcacttt tcacgattga atacggtgaa
    18841 attgaagtga ctgatattct tcaagacgat ctcttttcag ataatcacga agtcgaaaaa
    18901 attgaaacag tagaatttcc aattgaagaa ggagctttga ttattgcaac tgttgatgat
    18961 ccaaaacaga atgttttcac tcttgagaaa gttggggtga tttgatggtt aaagtaaaga
    19021 gaatgatgga gactgatgaa tctggtgtta aacgtcaatt tatgccaatt acacatgttt
    19081 ctgcagtttt aggcttagaa aaaatgatat caggacaatc aaaagttctt tcggttaatg
    19141 gtaaatatgg tgcagtcatt ttaacgaaag ctgatttagg attagagaat gcaattactg
    19201 aactacctta tgcaagcgag acaagcgatg gtattcttac tgctgaaatg tttcaaaaga
    19261 tcgtaaatgg agaaggtgga acatatattt tgccgattgc tactcctgaa caattaggag
    19321 gtataaaagt tggggaactc ttagaagtta ctgaagaagg tgttttatct gcaactaagc
    19381 aaacagactt caatttctct gaagaattaa agggaaaatt agaatctcta aaaatattaa
    19441 aagcaggtgc aaatatatca attgcagaag atggaacaat tagttctaca ggtggtagtg
    19501 ggactggtgg tgttaatcag tcatatgtag atcaaaaatt ccaagaagct gtaaatcaag
    19561 cagaaaatta cacaaacgaa agaattccaa attttacttt tgaaaaaatc ggggaggtat
    19621 agaaaatgac agatatcgta aaaataaaac aaagcggtgt acaggtttat ccacaaactc
    19681 attggaatgc tatagaaggc aaaccaacaa cggtgaaggg agataaaggc gatcctggcc
    19741 aagcagcaac aattaccata ggaaccgttt ctagtggttc aaccgcttct gttacgaatg
    19801 taggaacatc atctgcagct agatttaatt ttgttttacc aaagggagat aaaggtgatc
    19861 ctggaataaa tgccacaact acagctgtag ctacaactac agcaaatggg ttgatgtcgt
    19921 ccacagataa aactaagttg gatggaattg cagctggagc acagaaaaat ccaggtaatg
    19981 ctacaactac gacagcaggt ttaatgtcag caaccgataa agtgaaactt gatggattag
    20041 cgaatattac atttgagaaa gtggggacgg tttaatggct gatattgtgc aattaaaaga
    20101 agacggagtt gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt
    20161 ggtgcagaca gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac
    20221 aatcaataat aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt
    20281 tgtcactaac ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat
    20341 atattttgtg gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt
    20401 tccagaaggc tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc
    20461 tactttgaac agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa
    20521 ttcaagttat ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc
    20581 taatgaaagt agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca
    20641 atcaagaaaa tatccctgaa aactgtacag agattaaacc accagttcct aactggagac
    20701 caagatttga ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag
    20761 ggaatgctgt tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga
    20821 aggcagaaaa tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa
    20881 tcaagcaaga aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa
    20941 aaacgatgct tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac
    21001 aaggtgggaa gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt
    21061 ttcactaacg aacaagtaat ggttttcgtt ccattatgca ttacagacga agaagcagat
    21121 aaaatcatta ataaagataa gagcgcatct taattgatgc gtttttattt aaggtaaagg
    21181 agttgtcaca tgattaattt aggggaatgg ggaatgatag caggatcaat aaccgctatc
    21241 gtttctttga ttttattagt aattagaccg attgctgcat ctttctcgaa aattactgag
    21301 actctttcaa aagtaagcca caatttagat ttgctgacta aagatttaga atcgagcaaa
    21361 tcagatcgat tgatgattca tgaagaacta aagaaacacg atgaaagatt agatacacat
    21421 acagaaaaat tggtggaaca cacgcaacaa atcaaaactt t
//
LOCUS       DACEPW010000029        21274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEPW010000029 DACEPW010000000
VERSION     DACEPW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.029"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(104..1336)
                     /locus_tag="IW819_000881"
     CDS             complement(104..1336)
                     /locus_tag="IW819_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1244003.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="IW819_000882"
     CDS             1591..2160
                     /locus_tag="IW819_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1244004.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="IW819_000883"
     CDS             complement(2338..2778)
                     /locus_tag="IW819_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1244005.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="IW819_000884"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="IW819_000884"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1244006.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="IW819_000885"
     CDS             3732..4655
                     /locus_tag="IW819_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1244007.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="IW819_000886"
     CDS             4731..5870
                     /locus_tag="IW819_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1244008.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="IW819_000887"
     CDS             5863..6663
                     /locus_tag="IW819_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1244009.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="IW819_000888"
     CDS             6663..7490
                     /locus_tag="IW819_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1244010.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="IW819_000889"
     CDS             7468..8202
                     /locus_tag="IW819_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1244011.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="IW819_000890"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="IW819_000890"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1244012.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="IW819_000891"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="IW819_000891"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1244013.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="IW819_000892"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="IW819_000892"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1244014.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="IW819_000893"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="IW819_000893"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1244015.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="IW819_000894"
     CDS             11787..13820
                     /locus_tag="IW819_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244016.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="IW819_000895"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="IW819_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="IW819_000896"
     CDS             15353..16159
                     /locus_tag="IW819_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1244017.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="IW819_000897"
     CDS             16171..17391
                     /locus_tag="IW819_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244018.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="IW819_000898"
     CDS             17381..18967
                     /locus_tag="IW819_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1244019.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="IW819_000899"
     CDS             19006..21144
                     /locus_tag="IW819_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1244020.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6685 a         3615 c         4472 g         6502 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctcc
//
LOCUS       DACEPW010000030        20963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEPW010000030 DACEPW010000000
VERSION     DACEPW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.031"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..657
                     /locus_tag="IW819_000900"
     CDS             <1..657
                     /locus_tag="IW819_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1244021.1"
                     /translation="FKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAE
                     VIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQER
                     IDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISEL
                     EKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV
                     "
     gene            complement(1360..1575)
                     /locus_tag="IW819_000901"
     CDS             complement(1360..1575)
                     /locus_tag="IW819_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1244022.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1594..1830)
                     /locus_tag="IW819_000902"
     CDS             complement(1594..1830)
                     /locus_tag="IW819_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1244023.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2088..4241
                     /locus_tag="IW819_000903"
     CDS             2088..4241
                     /locus_tag="IW819_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ1244024.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4210..5625
                     /locus_tag="IW819_000904"
     CDS             4210..5625
                     /locus_tag="IW819_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ1244025.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            5658..6692
                     /gene="sppA"
                     /locus_tag="IW819_000905"
     CDS             5658..6692
                     /gene="sppA"
                     /locus_tag="IW819_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1244026.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            6694..7215
                     /locus_tag="IW819_000906"
     CDS             6694..7215
                     /locus_tag="IW819_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1244027.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7212..7598
                     /locus_tag="IW819_000907"
     CDS             7212..7598
                     /locus_tag="IW819_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1244028.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(8094..8166)
                     /locus_tag="IW819_000908"
     tRNA            complement(8094..8166)
                     /locus_tag="IW819_000908"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8131..8133),aa:Lys,seq:ctt)
     gene            8547..9116
                     /locus_tag="IW819_000909"
     CDS             8547..9116
                     /locus_tag="IW819_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1244029.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            9103..9963
                     /locus_tag="IW819_000910"
     CDS             9103..9963
                     /locus_tag="IW819_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1244030.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            10153..10857
                     /locus_tag="IW819_000911"
     CDS             10153..10857
                     /locus_tag="IW819_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1244031.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            10862..12697
                     /gene="walK"
                     /locus_tag="IW819_000912"
     CDS             10862..12697
                     /gene="walK"
                     /locus_tag="IW819_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1244032.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            12694..14010
                     /locus_tag="IW819_000913"
     CDS             12694..14010
                     /locus_tag="IW819_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244033.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            14011..14880
                     /locus_tag="IW819_000914"
     CDS             14011..14880
                     /locus_tag="IW819_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244034.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            14935..15744
                     /locus_tag="IW819_000915"
     CDS             14935..15744
                     /locus_tag="IW819_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1244035.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(15847..16476)
                     /locus_tag="IW819_000916"
     CDS             complement(15847..16476)
                     /locus_tag="IW819_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ1244036.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            16646..17938
                     /locus_tag="IW819_000917"
     CDS             16646..17938
                     /locus_tag="IW819_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1244037.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(18014..18676)
                     /locus_tag="IW819_000918"
     CDS             complement(18014..18676)
                     /locus_tag="IW819_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1244038.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            18901..19185
                     /locus_tag="IW819_000919"
     CDS             18901..19185
                     /locus_tag="IW819_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1244039.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            19236..20861
                     /gene="groL"
                     /locus_tag="IW819_000920"
     CDS             19236..20861
                     /gene="groL"
                     /locus_tag="IW819_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1244040.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7153 a         3404 c         4234 g         6172 t
ORIGIN      
        1 tttaagatag tattgaaaac acaaagtccg ttagaagtta aaaaatacct caaacaattc
       61 cgaccaaagc aaggaatata tttcttagat attgatttaa atcatgaagt taacggtata
      121 gaattagcag aagtaatcag aaaatatgat gttcaagcaa aaatcatttt tacaactact
      181 catgatgaga tgttacccgt aacaataaaa agaagagttg aaacgttagg atttgtaaca
      241 aaagatcaaa cactagatga gtatcgaaac gagattgttg agttattgtt attagcgcaa
      301 gaaaggatag atgcaacaaa acaggttacg aatcaagcat ttgttttttc cataggttct
      361 caaacattta cttttgatat aaatgacatt tattttgtag aagcttcaaa attaccacat
      421 agattatcat tgtgtactaa agatgggcag tacgaatttt atggaagaat tagtgagctt
      481 gaaaagaaat atcctatgtt aacaagaatt agtcgagcgt gtttagtaaa tatctttaac
      541 gttaaagaaa tagattttaa aaaacgttca ctatattttg attcagagtt ggccagaaac
      601 tttactttag ggaaagcaca aaaaattaag gagaagttga aagaaagcac tgtttagtgg
      661 ttttttcttt agggagattg catttttcta ataaaataga ttccttcact agtgcaaatt
      721 tttctgaatc ctattaaagt tgtaccaatt tttatagctg ttattagtta cgtgcataac
      781 agcaatatta gttactggag gaatatttct atggtctgga accaaaaaag ggccaaatga
      841 atagttcttt tcttgttttt attaagtacc ataaagggga attaaaatta aatgtatggt
      901 ggaagatgcg aaagatttgg caaattctac taatttttag aaagtgtttt tcatttttta
      961 gttaaaaaat ttgtcctgtc aagtgcaatc ctatgattgt acttgatagg atttgagata
     1021 attactttcc tgtttttata aatttaactt gcaggaacag gctttaatgc acatccgaca
     1081 agtacattac caatacctcc tgtaacggcc gaaataggtc ctcctgcagc accaactaca
     1141 gcgctgcaag tccttttgta aaaatatctc taccattaca tttccaaaga atttttatct
     1201 gactttaata aaagataaaa atttaatata attataaaaa ctagcacaag aagctttagt
     1261 aaaaagttct gtccattcca aacttttgaa aagataaaga acatacctat gttcgtaatc
     1321 gacacaaacc aatcttgagg tttgatattt tttaattgtt tacgcaccgg tccacatgct
     1381 gaatccagca cctaataaag ctccacctac accacaagca gctattgcat aaggtcctcc
     1441 ggctgaacaa agttttactc ctgctgttgc cccagaaaaa gtagtgccta aaactcccac
     1501 ggcactccca ccttgaatat ttttcaactc ttctgaagat aatataaatg ttgtttttgt
     1561 gtttttaata tgcatactac ttattctcct tttttatttt tttcgagcat cccagccagc
     1621 tgcagcacct ccagcgacag ctccatataa ccctccaaca acaccgcaag ctggtccgca
     1681 aattgctgtt cctaaacctg caccacctac tgcccctgtg gtaagtccac ctacaacttg
     1741 ccccaatttt ccccaagcat tattgcctcc accaacagtt tgtgcatttc cacaatactt
     1801 agttccttta cattttgcat gtttaaacac gcttctttaa ataatatttt ttacggtgct
     1861 actgtaacat acgtatttga ttaacagtaa aaatcttcct gaaacttatc aagtttattc
     1921 ttgaatagta aaatttccaa aataaatttg attcaggaat gaaaaagtta gtgttcaaga
     1981 actgttttca aagtttttgt ggaattatga gacaatattt tcactgtatt aaagctatat
     2041 tgagaatttt tctataaaaa ttttttagaa ggagttgtgt tagataagtg ataaaatcat
     2101 ttccttttgt gcagcaacaa gatgaaaaag attgtggtgt tgcttgcctt tcgatgattt
     2161 taaaatattt taaaactgat atcccgattc ataaattacg ggacctatct gggactgatt
     2221 tagagggtac ttcagcattt ggattgaaaa agacatttga aaaattgtct tttgattgta
     2281 tggctattca agcagatagt gagatctgga aagaaagaga tcttatctta ccattaattg
     2341 cacatatatt ggttgataac agctatatgc actacgttgt aatttataaa acgaagggaa
     2401 acgtactatg gattgcagat cccgctaatg gaaaaaatag aaaaacgatt gaagcgtttc
     2461 aaaaagaatg gacaggtatt cttttattac ctatgcctag agaaacttat attcctatta
     2521 aagaaaaagt agctggatta agttcttttt tgcctataat ttggaaacaa aaaggattag
     2581 tattccacat cgttttagca tcgattttca ttacgttttt tggcatagga agctcatatt
     2641 attttcaagg tattcttgat tttttcatcc ctaatcaagc acgttcaact ttaaatataa
     2701 tttccattgg cttgatcgct gtctatttat ttcgggtatt gtttgaatat agtcgtagtt
     2761 atcttcttat tattttagga cagcgaatga gtatggcaat tatgcttcag tatttcaaac
     2821 atgtgttaac tttaccaatg aatttttttg ctacgagaaa atcaggggaa attatttctc
     2881 gttttcttga tgccagtaag attattgatg ctttagcaag tgctacgtta tcaatatttt
     2941 tagatattgg tatggtgttg cttgttgggg ttacgttagc tattcagaat agtacgttgt
     3001 ttttaatcac tctatgctct ttgccttttt atgtagttgc aatattcgct ttcgtgaaaa
     3061 gctatgaaaa atcgaacagg gaagaaatgg cagccggtgc agctgtaaat tctagtatca
     3121 tagaaagtct taaaggaata gaaacaatta aagcatatag cggtgaagag aaagtatata
     3181 accgtataga ccatgaattt gttaaattaa tgaaaaaatc atttcgtacg gttacgcttg
     3241 ataatattca acaaggaatt aaacatttga ttcaattaat tagcagtgca ttaattttat
     3301 ggctaggttc ttattttgtt atggataata gaattagtct aggtcaactt atcacataca
     3361 atgctttact agtatttttg acagatcctt tacaaaacat tattaattta caagtgaaaa
     3421 tgcaaacggc tcaagttgca aataatcgtt taaatgaaat tttcgcgatt gattcagaaa
     3481 aatcgaacca gtcttttgaa tatatgcttt ctgaacaatt atttcaacaa gatatcactg
     3541 taaaaaacgt ttcattctcg tatagcatga aggccccaac gttgaacgaa atttcatgta
     3601 caattccttc gcacagtaaa acggctatag ttggagttag tggttctgga aaatcaactc
     3661 ttgcaaagtt atttgttcgt ttttatgaag catcagaagg atcgatccat tatggaaaca
     3721 ttaatcactt agacattcct tttcagatat tacgcgatcg agtgacatat ttgcctcaag
     3781 agtcattttt ctttagtgga acaatcattg agaatcttat attcggttta gatagcccac
     3841 caagtttcga acaaataata gaagtttgtg agatagtcca actaaatgaa tttattaatc
     3901 aacagccatt acgttacgag acattgttgg aagaaggtgg aaataatctt tcaggagggc
     3961 aacgacaacg attagcctta gcacgggctt tattaaaaaa tccggacata ttaattttag
     4021 atgaagcaac tagtggatta gataccttgt tagaacatgc aattatgaat tatctcctca
     4081 atttaaaaga aaaaacgatt atttttattg cgcatcattt accaatagca aaagcatgtg
     4141 atcaaatttt agttctccat gaagggcatt tggtagaaaa aggaacgcac gatgaacttc
     4201 gttttgccga tggtatctat caaaaattat gggaaatcta ggagagggaa acaatttatg
     4261 cagaataata attggttcga acattctagt atctatacta aacaacatca tagcttctac
     4321 cgttgggtgt tataccctgt aattcttttt ttatgtatta ttggattgtt tttaaccttt
     4381 gctaaaaaag aggttgttat tcggacaacg gctcaattga accccgaaaa aatagagaag
     4441 ttacaagtgc cattagaagc taaaataatg gaaaacttcc taaaagaaaa tcaatttgtt
     4501 cataaggacg atgtgttggt tagattggat tgttctttaa tagagaatga aaaggctcag
     4561 atagaacaag aaaaccaacg tattactcaa caaataaaga tggctcagct atttattgaa
     4621 agtataagta aaggaaaaaa tttgttttca acggatgaca gttttggcta cagtaatcaa
     4681 ttaaagagca tgttgtcaga aaaagaatca ctccgctacg ctttgaagca aagtgaatta
     4741 aatgatcaaa agcaattaga agtatacgaa aagacaaaaa gacaactaga aaaacaaatt
     4801 gagagttcag atagtaaatt acaagaatgg caacaagtac aggtagcttg gagtaataat
     4861 caatcattaa aagatttttc aaaagaaatg atggcaaact atgagaattg gcaagaacaa
     4921 ctaaataatg tttctgatga tcaaaaaaat caagtgaaac tgacaatttc agcaagcata
     4981 aatgaacaaa ttgagcaact aaaaaaagaa gtagaacagt atcagtcaga aaaagctaaa
     5041 ttagttaaac caactacttc tgagaatgac agaattagtc aaacggaaaa aggaaagcaa
     5101 gagctagaac aagcaataac aactgtaaaa caaacagtta cagagctaaa tgaaaaacaa
     5161 gagaagaacc agtcagttat caaaagtttg gacgatcaac tttcaaaagg aatactaaaa
     5221 gctcctgtat ctggaacggt tcatttaaat gaagaaacaa agggacaagc agaactagcc
     5281 aaaggaacag tgcttgcaga aatttatccg atccacgaaa aatcaaaaat gaaatttaca
     5341 gcacttttac ctgcgaatga aagtacctat attaaagaag ggatgaaggt tcattttaaa
     5401 ttggatcaaa aaggtggggc tccgattacg atcgacggaa ctttagatga aatttcagaa
     5461 aattctactg ccactgagaa aggtgttttt tatgcagtga aaggaacgtt aaaaacaact
     5521 caatcgtttc cgtttcgtta tggattaact ggagagttgt ctttaattgt aggagaaaaa
     5581 acatattggc aacaaattgt cgatgtgttt ctaaataaaa aataattatt tttactaaga
     5641 atataggagg agtttatatg aataaaagaa gatggattgc tgtgggagta gcgtgcggat
     5701 tattgttact atccattatc gtcgcgctaa ttccaggaaa agataaagag gaagcatcaa
     5761 atacaaccct aacaggaata aataagattt tttacggatc taacgaaatc acagaagaaa
     5821 cgttagaaga aggcgcatca aataaaaaga ttgtcaaact atccgtcaat ggagttattg
     5881 cagatacagg tgaaagcaat ctttttagta gggaacaata taaccatcag aattttttaa
     5941 cacagctaaa gaaaattcaa gaagacaaag cggttaaggg agtgttatta gaagtaaatt
     6001 cacctggggg aggaatatat gaaagtgctg agattgctaa agaaatggca aaaattaaaa
     6061 agctagatat tcctatctat actgcattta aaaatacagc ggcaagtgga gggtactaca
     6121 tttctgctgg ttcagataaa atatttgcta cggaagaaac aacaactgga tcaattggtg
     6181 tcattatttc tggtttaaat tattctggct tattagaaaa acttggcgta actgatgcga
     6241 cgtataaaag tggtgcatta aaagatatga tgtcacctca acacaaacct tcagaggaag
     6301 agaataaagt tatacaggaa tttgtgatga gtgcttatga tcgttttgtc aatgttgtag
     6361 caaaaggaag aaatatggat actaatgcag tgaaagaact tgcggatgga agaatttatg
     6421 atggtaacca agcagtagaa aatgggttgg tggatcaaat tggttactct gaagatgcat
     6481 tagattccct aaaaaaagag aagaaattaa ctgatgcgac ggttatagaa tataaaaatg
     6541 acacaactgg ctttgcttct tcttggttag gtaataagat tgctgaatgg caagggctaa
     6601 aggcaactac atcagatcgt gtaatttcag tttttgaaaa acttggattt tctgatactc
     6661 caaaaccaat gtattactat ggagggctat aaaatgaata agaagagtgg cattgaaact
     6721 ttgcctaatt atgtatttgc gggattttgg ttacgatttt ttgcttttct tattgattta
     6781 atttgtatta gcatcatcac ccatgctact ataggtctct tttacaaaat catgggactg
     6841 gaatctgaca aatccattat tactttttat ggtatagcgt cattagctat ttatttaacc
     6901 tattttgtac ttttgactaa gctgaattca ggacaaacaa ttgggaaaat gatttttggg
     6961 ataagagtag tgagctttga ggaaaaagag ctaagttggc aaactatctt tattcgtgag
     7021 tgtatatgta ggtttatatt aaaaaatttt gtatttcaaa ttgggtattt ggtggctgct
     7081 ttcacgccca agaaacaaca tattggggat tatttttcta acacgtcagt ggttgttatt
     7141 cgttcattag aagctattaa ctatggtcaa aaaatttcta attctaaaac acttaatctg
     7201 gagagaatta aatgaataag agaaaaacag agtttatttt gattttaatt agtggaataa
     7261 cagggatttt atgtagtctt ttagcattga tgggtggtat gtttctatct tctattgatt
     7321 caactacttt aaatactaca gaactaacca gtaaggatat agatatgtta gtcaatctaa
     7381 gcgggaatgt tgttttattt ggaggaatta ccctagtttt ttcgataatg attttcataa
     7441 taggtttttt agtaagaaaa aatcaaaaaa cagtggtcta tggtattagt ttactcattt
     7501 tatctattat tccatttttt cttttaacat tattgtgggt aattcctgga gttttaggta
     7561 ttattgcggg attgatgctt ctaataagaa aatcttaatt aggcaatgtg tagacaacgt
     7621 gaaacgatta atcaattaaa agctaaatac gttttgagaa atgataactt gtagtcgatg
     7681 gaagacatgt agataaaaaa actacgtgtc ttttatttat tctaattttt tcgaaaaaaa
     7741 gacactctct gattgttgta ttaatggaaa aggggaaagc agcaagtaaa tttagttttt
     7801 ctattaaata ttcttcactt tttaaccttt tatagtactt agatttaatc ctatatgttt
     7861 agagagttca ttggcagcat gattttgcaa agcttctcga attattgtta tattagaaac
     7921 tttactgttc ataattatgc ttctttttta actcaaagtc caaagggcta cagtggactt
     7981 tgagttaaaa ataaaaagcc ctagttattt tgaaaaataa aaaaagaaca gcgtaaatgt
     8041 tttcgaaatg ctttcgcacc aacatttacc tgttccgtgg gtttggcacc caatgggccg
     8101 tgaggggctc gaacccgcga cccgctgatt aagagtcagc tgctctacca actgagctaa
     8161 cggcccgtac aaaaagtact taataatagt agcactaaga attttaaaat gcaagactac
     8221 atttaaagaa aatcagaaaa atctttactt ccttatagga aatcattcat agattattca
     8281 catttgctcg ctattatata agcataccaa caaacaaagt tcttgcaaga gaacaaaccc
     8341 taacactttt ttcatttttt actcctttcc cgccgtctcc ccggcgggtc tcctgttctt
     8401 aacgataaaa gacaagaagg cttagccttc ttgttttttg ttttcatgga atcccgctaa
     8461 tcaaatcacc tagttaaagc aactagattg aatgtttatc ataagtctga tatcatgttt
     8521 atgaaatgaa aaaaggagcg gttatgatgg aaaaaatgcg tcagcaatta atggagtggg
     8581 ggaaggaact agaaacattc cgtctgcctc actgggaaga actccctgac ttagaacttt
     8641 atatggacca agtcagaacg ttagttgatc gttatctttc acctgtgata caaggagaaa
     8701 agcatccatt gcttacctct tctatggtaa acaattacgt taaactaggt ttgataccag
     8761 caccagttaa aaaaagatat aacaaagaac acgtagcatt tttacttgca atcactacgt
     8821 tgaaacaagt attgactatt cctgaaatca aagaagggat attgttccaa ggaaaaacag
     8881 taggaattcg tgaagcctat aatctatttt gcgatgaaca agaagcagct gtgtggatgg
     8941 tcagccaatt agcacaaggt aaatctcatc cgcaaaaatt cgaccaggca acccctgtag
     9001 aatatattgc tgtaagagcg gcaactttat cgtttgcaat gaaacttctt gcggaaaaaa
     9061 cgattgttct agaaaccgaa tatttaaaag aggagaaaac aaatgaaaaa caataaaatc
     9121 gcaattttag tagattcagg tacggatgtt ccagaagaat tgataaaaaa atacgagatg
     9181 tatatgatcc cattgaaaat tatttataaa gatcaggtat atacagataa aattgacatt
     9241 acccctgaag agatctatca acgtttgcca gaggaaatcc ctggtacatc tttgccggat
     9301 ggtgagtcga tcacagaaat ctttgagcaa atcaaaaaag acggttatga aaaggtactt
     9361 gcggtaacaa tttccagtgg attgagcggg acatataatg tggttcgttt aattgcacaa
     9421 cagcaagaag aattggaagc atatgtacta gatacgaaaa atattggtat tggtgcaggt
     9481 ttttctgcga tccaagcagc aaaatggctg gaagaaggag tagaatggga tcaactgatc
     9541 agtaatctga atgagttagt cgaaagaaca aaagttttct ttaatgttgc gacattggaa
     9601 tatcttcaaa aaggtggacg gatcggttta gttgcttcta ttgtaggaac cgctttaaag
     9661 cttaacccaa tcatctcatg taatgaagaa gggatttatt acacagtagg aaaagctagg
     9721 ggacgtaaaa aaagtttgga taaaacggtt gcttacgtca aagaaaaaat cggtaatgca
     9781 aaggtcttca acttagctgt tgcccatggg gatgcgaaag aagaagcaat tgaaatggaa
     9841 gcgagactta aaagagagtt cccacaagca aaccaaatct actttggcca gatttctccg
     9901 gcattagtag tccacactgg tccgggatta ttgggtgtcg gtgtacaagt tttagaagga
     9961 taaatcaaaa aaaggaaaca ctgaggtgaa aatcagtgtt ttcctttttt attgaagtta
    10021 gaacaaaaaa taataaagta ttttgcactt tcattttaat tttctctttt gttttatagg
    10081 caaagatgaa acaaaagcag aaatacggta taatgagaag gtatgacaat aatatataag
    10141 aggtggctct ttatgaaaaa agttttagtt gtcgatgatg agaagccgat ttcggatatc
    10201 gtcaaattca atttagccaa agaaggctat gacgtttata ctgcatatga tggggaagaa
    10261 gccttggaaa aagttgctga ggtagaacct gatttgattt tacttgattt gatgctacct
    10321 aaaatggacg gcttagaagt tgctcgtgag gtacgcaaaa cttatgatat gccgatcatt
    10381 atggtaacag ccaaagattc tgaaattgat aaagtcctag gcttagagtt aggcgccgat
    10441 gattacgtga ccaagccatt ttccaaccga gaattagttg cacgtgtcaa agcaaatctt
    10501 cgtcgcggtg ccacggctgc gaaggaacca gaagaggcag cacctgcgga attgacgatt
    10561 ggtgatttga cgatccatcc agaagcttac atggtgacga aacgaggaga aacaatcgag
    10621 ttaacacatc gtgaattcga actactcttt tacttggcga agcaccttgg acaagtgatg
    10681 acaagagaac atttattaca aacggtttgg ggttatgatt attttggaga tgtacgaaca
    10741 gtggatgtaa ctgtacgtcg tttgcgtgaa aagatcgaag ataatccaag ccatccaaat
    10801 tatttagtga cacgtcgtgg tgtcggttac tatttgcgta acccagaaca ggagtaattg
    10861 gatgaaaaga aaagtccgct tttttcgatc agtcaatttt aagatcgcta ttacctttat
    10921 tctgatttta ttgatctcga tcgaaattat tggtgcctac tttatacgtg gtttagaacg
    10981 ttccacgatc aatacattta ttaaagatat gaatcaaacg gtggaatcat tagcaacaac
    11041 aatcagcccg gaattgaatc gaaaagataa tgcagatgat gaagaggtaa atgcgaatat
    11101 caaaagattt attgaaaaca gtgcgactag cgatatcatc gaaatacggg tcgttgacga
    11161 aaaagggatc atccgaggaa caaccgatgt caatgaacaa agcgctgtgg gaaagaaaaa
    11221 tgattatgtt gatatcaatg atttcacgac taaacgttat gttgctttag ataacgacga
    11281 taaacgggtg aatatcaatg ttcaaccgat cctgtccccg accggcgatg ccgttatcgg
    11341 ggctctttac gtcaaaagta atatcgaaca aaaatattcg gaaatcaata acacagcagt
    11401 cattttcttt actgcctcac taattgcggc atttatttca atgatcgtat ctgttttagt
    11461 tgctcgttcg attacacagc cgatcggtga aatgagagaa caagccattc gaatcgctag
    11521 aggagactac agtcggaaag tcaaagtgca tggacaagat gaacttggac agctggctga
    11581 taccttcaat cagttagctg aacgtattga agaagctcaa gatacaatgg aagctgaacg
    11641 caatcgtttg gatagcgtgc tatcccatat gacagacggc gtcattgcga cagatcgcag
    11701 gggtaaagtc attacgatca atgatatggc tgtttccttg ctagatatca aaaacgagga
    11761 agcaatcgga caatcgattt tgacactttt agatatcgaa gaagaatata cgttgcggaa
    11821 attattagaa gatccaaacg aaatggtact tgaccgttca accagtacat tggaaagtga
    11881 tcagatgatc ttgcgagtag acttttctat gatccgtcgc gaatcaggtt ttatcagcgg
    11941 attagtagct gtcttgcatg atgtgacaga acaagaaaaa acagaacggg aacgtcgtga
    12001 attcgtctct aatgtttccc atgaacttcg tacaccgttg acaagtatgc gtagttatat
    12061 cgaagcattg agcgaaggtg cgtggaaaga cgaggaagta gcaccaaatt ttttgaaggt
    12121 aacgctagat gaaacagacc gaatgattcg aatgatcaat gacctcttaa atctatctcg
    12181 catggataca ggtaataccc agcttcaatt agaatatgtc aatttcaacg agttagtgaa
    12241 ttttgttctt gaccggtttg atatgatggt gggaaatcag gaaaaaaatt ataagatccg
    12301 tcgtgaattt acgcaaagag atctttgggt cgaagtagat acagataaaa tcatccaagt
    12361 agtcgataat atcatgaata acgcgatcaa gtattcacca gatgggggaa cgattacttg
    12421 tcgcttatta gaaacgcata ataacgtgat tttaagtatc accgatcaag gattggggat
    12481 tcctaaaaaa gatctgaacc gagtgttcga gcggttctat cgagtagata aagcacgtgc
    12541 aagagcacaa ggtgggacag gacttggatt agctatttct agagaagtga ttaaggcgca
    12601 tcgaggcgct atttgggcag agagcaagga aggaaaaggg tcgacttttt atatctcgct
    12661 gccatatgaa ccttatgagg aggaatggtg ggaatgagaa tctctgaaaa agtcgttcgt
    12721 ataggactca ttttgatggt tgcgttaagc atatacttct cttatgcaat ctggctgagc
    12781 ccagcaggaa aaagttcaat caacgtagat gagacaaaca ctcaaatgat cgagtctcaa
    12841 agcaatcgaa aaacttccga agtctttctt ccgcttcatg ttacatggca ccattcggga
    12901 atgatccaag aaaccaacag tgaaaatctc gtgtaccgat tgcaattgat catcgataaa
    12961 gcaagatttg gaaagctttc tgaagtagtt tcaggagaca aatcacagtt tgaacaggtg
    13021 aaaaatctga actctggttt tgagttggcc tataatgcgc cttttttgct aagagaatac
    13081 aaggaagcat ttgatttaga tttagatgtg tcgaacttgg caaaagaaag cgaaaatatg
    13141 tattttacta gaatacaatt cgatcaagga aataaaaaaa tccgattttt gaattattcg
    13201 aaccatctcg tatatgaagc aaatatttcg ttgaattgga cagatatcaa taaagaatta
    13261 acagcaaaag acacaaaatg gacagaaatg acaacagaac catcgacgat cgacacgcaa
    13321 tatgctacga aacattcgat caaactgaag aagtatagtt atatcctgtc acagcaaccc
    13381 tatacacttt ttagaaatgc ttttttccag aaaccagata gcgtgaaaaa taatgatgaa
    13441 agcaatgaat tgatttttta cggtgggaat gaaacaatgt ctatccattc ggaaacgcaa
    13501 gtagttgatt ttcgtggaga gctgccagag aaaaaagaaa atgctagtgt attttctgaa
    13561 agtttcccct atattagcaa attaggcagt tctttaggga acctcagata ttttgaccgc
    13621 agtgacaata cgatcgatta tcggatattc gtggaaggct tcccggtttt tgggacagac
    13681 agcagaggac agattggtgt ggaagttggc ggtgaaacga atggttctgt gcaagtgaat
    13741 atccaaacca gtttgaatac gattcaagtg ccgatacctt cagacgaaga agtagaactt
    13801 gcaagtacga atgaaatcat ccagcagtta aaagctaagg gagcagatag caacaagatt
    13861 ggttcagtga tcgttggcta tacatggcaa aatgtcaaag aaacgaatcg tgtcgttgat
    13921 ctcttacctg aatggtatgt gaaatataaa gataactggt attcagctaa cgaccttctg
    13981 gaaaaattac ctggattgga ggcgaattaa ttggatttca aaaaaatcga atggatattt
    14041 ttcgtcgctt ttttagggtt gaatgtgttt ttattcagca gttatcatga cgcggtatac
    14101 caagaaaaca atgtcattcg ctctaatcag actgaatcaa tccaacaacg tctggctagc
    14161 gacgatatca cttacacggg gaagatttcg tctgagaaca agcaaggcta ttatcttagc
    14221 gcggagcaga ccaatcttag cacttcatta gcgaattatc gcaaggctgg taaaagcggc
    14281 ttgctggcaa gtggtgtgtt tttagacggt gatgttttga cgaaatcttt atccagttca
    14341 gatgctgaaa agaacgtgat cgatccagat aaaattagta gttccttgga agatttttta
    14401 tctaacaaag acaatatttt attcggtaat gattatgtgt acacgaaaaa cttttctcat
    14461 ttagaagaag aaccactaga aatccaagca agccaagagt atgaaggcat cccattcaat
    14521 gatgatacag caaaaatcgt tctttcttta gaaaaaaacg ataatgatta tagcatcacg
    14581 aaatacatgc agacgcattt atctgatatc gagcagctgc gcgaaaagac ggaactgtat
    14641 acggaagagg aagcaatcaa tacattgtac atcaataaca aaataccaag aggatcaaaa
    14701 attttatggc gtcagctagc ttattcctgt attctgaaag tccgggaaaa aaatgtgtat
    14761 gttcctgttt ggcatgtagc gatcgaaaca agcgataaag tggtccaaat tgagagcatc
    14821 aacgctttca gcaatacgat cgtgacaaac aatactgtac caaaagtgga agatcaataa
    14881 aaaagcgagt ataatgaaaa aagtttacaa agtaagaagg gaagtaggag tcaaatggct
    14941 gaagaaaaac cagcttttaa aatcagtatc ctagcaagtg gaagttcggg taattcactt
    15001 tatatcgaaa gtgaaaaaaa acggcttttg gtcgatgcag gacttagcgg taaaaaaatc
    15061 acttccttga tggctcaaat tgatcggaca ccggaccaat tagatggaat acttgtgacg
    15121 catgaacatc gagatcatat acatggcgtg ggtgtcttgg ctcgtaagta tcatttggat
    15181 gtttttgcca atcagcagac ttgggaagcg atggaaccac tgattggaaa tgtaccagtg
    15241 gaacaaaaac atctatttga gatggggaaa gtcagaacat tcggcgatat cgatattgag
    15301 agtttcggcg tctcccatga cgcagcggct ccgcaatttt atcgtttcca taaagaaaat
    15361 cggtcattcg tcatgttgac ggacaccgga tattgcagtg attatatgcg aggtgtcatt
    15421 gaaaatgccg atggttattt gatggaaagt aatcatgatc ttgagatgtt acgcatggga
    15481 ccttatccgt ggaatttaaa acagcgtatt ttaggagata gaggacatct ttccaacgaa
    15541 gacggtgcac ttgtcatgac tgatgtgatc ggtaatcgga ccaaacgaat ctatcttggc
    15601 catcttagca aggaaaacaa tatgaaagaa cttgctcatt taacaatgga aaatgttttg
    15661 aaagaaaaag attttggcgt tggacatgat tttgaaatct atgatacaga tccagatgaa
    15721 gcaactgagc tattttcgat ttaaacagtt caatcaataa aaatataaaa agcagaagat
    15781 aaaatcaatc agggttggtt ttatcttcta tttttatttt tttagcatat taaaaaaata
    15841 accgtattat cgactgacta cactgatgtg ctgcttttca tgtttctcat ccagtatctc
    15901 atctactaat gcaatcgcgt aatctgccat gctgatcgta ctgtcgctag cagcgtttct
    15961 ttgtaaataa tcatcactta acaaataagt acctgtacgt ttgccatgtg gatcaaataa
    16021 agaagctgga ctgatataag tccaatgtag atttttagat tgtttcaatt ttaacaatcc
    16081 ttcgtacata ttgaaagctg ttggatagaa gggtgcattt ttaggtactt ggtcaatcat
    16141 acgagtcgtc ttttctgtat ctaagaataa cgaagaggca cccccaacga caattaagcg
    16201 tgtatcggtt ccttcaagca tctcaatcaa atgggacaaa gaagtttgat gcatttcttc
    16261 ttttcctttg ggagcgttga aagcatcaac gacaacatca aaagatgcaa gatcagtagt
    16321 tgtcaactca aataaatcct tttgtataaa cggaacatct gatggcaatt tttctggatg
    16381 acgcacgatt gccgtcacgt ctaaatctct ggacaatgct tcgtctaaaa taaatgaacc
    16441 tgcatggcca gtagctccaa tgagtgcaat tttcataaaa aacctccttt tttattcctg
    16501 tccattgtaa gcagttgctg aatattcgtc aaagaatccg atttttgaaa agctcagtgc
    16561 gcattccttt catggttcgt gatcattggg tatagttaaa ttaagaaaac tgattgataa
    16621 aaatagaagt tgaaggagat aagaaatgaa agagtggtta gatcgtgcct ttttaccaat
    16681 gataacatct gttgtgaatc ttagaccctt aactgctttg cgtaatggaa tgacttacac
    16741 gatcccaata attattgttg gctcgctgtt tttcttattt gctcattttc ctgtggatta
    16801 catcgctcag tggatagaca aaacaggatt ttctatgtat tttacacaag tataccaggc
    16861 tacatttgat gtgatagcct tttggatcgt ttttgccatt gcatacgctt atgcaaaaga
    16921 agaagacatc gatcgcttac cagtcggcat aacagccttg tctgcttatc tactgcttat
    16981 tcagccacta aatactcaga tagatactgt ctggacaggt gctgaaggga tactaggagc
    17041 agttacaact gggctcatca tcggatgggg ctatgcacgt ttagtaagac ttgcgaaagg
    17101 gtggaagcaa agagagaaga tacctgaaaa tgtttggcag tcttttactt cattgattcc
    17161 aatcattgtt atagtcactt ttacgcttat cggttcagcg gtttttcaac atttgttcag
    17221 aatgactgtt gttcaaggag tatggacata tgtccagctt cccattcaat cgttcataga
    17281 cacgttgttt ggtgtgcttt tattaggttt ttttgttccg tttttatggt tatttggtct
    17341 tcatggatct gcgatggtca atgggttggt tagtcccgtc ttgcaagcga attcattagc
    17401 aaatgcagaa atcttagcct caggacaaga gttgacggtg gcaaatggcg gacatatcgt
    17461 tactcagcaa tttctcgatc agttcatgac ggttacgggt gctggattga cgttaggggc
    17521 tgtatttttt atgacggttt ttgcgaaatc gaagaaatac agagaattag gaaatcttgc
    17581 tcttttacca gcctttttca acatcaatga atcaattatt ttttccacac cgatcgtgat
    17641 gaacccgatg atggctgttc cttttatttt cgcaccggtc gtttcaggat tgattaccta
    17701 ttccgcttta tattttggat tcgttccttt atttacagca gtccaagtcc cttggacaac
    17761 accgccgatc ctctcgggtt tcttagtagg cggagtgcct gcagcgattt tacaaggtat
    17821 cgtgttgtca attagttttt tcatttattt cccatttttg aaaaaaatag attctgcaaa
    17881 ctgcaaaaag gaaagacaga caaaaaatga tggagcagca gaaaagatga acgactaaaa
    17941 aacaaaaata ctcctgtcgc tttagattgg gaaaatcacc aatctaggat gacaggagta
    18001 tttatagtca tgtttatact agcccttttt ggacagctaa ttgaacaaga acaactaatg
    18061 tgttcattcc tacgtgtgtg atcatcgatg tccagatccg accagttgct ttgtactcta
    18121 ggctgaagaa gaaacctagg aaaaagtaga ttagcagatg accatcatta tgagcaaacg
    18181 caaacagcaa agagctgatc actgctccga cccaaaaatt tgaatatcga ctgatgatcc
    18241 ccagtatact gcggcggaat acgaactctt ccatgatggg tccagcaaca gttgtcgcca
    18301 aaataaaagc gggtgcttcc atgatcatct ggatgatatt ttgtgtattc tcggaagggg
    18361 ttgctttgcc aaacaataca gattctaaca tgacagcgat gctttgcaga atcaatgcga
    18421 caaagattcc aaccaatcca taaataacaa tctttggagc agaagctttt tttaccgctg
    18481 attcgatcgc taagggttca cgttgcttaa aataaaaaat agctaggaca acggcgccta
    18541 gtatatacat gactgcagtt gctgtgatca attcagacgt agattttaca agtcctatcg
    18601 aagctaggat caagggtgag ataaaaatta gaccatagca aaaaatgctg aggaagctat
    18661 atttttttaa agacatgtaa ttcctccttt taagggataa caagcatgca aggcaagctt
    18721 atatcgattg atttcaatta tatcataatt tcaataagca gctgtcagaa taaaatttct
    18781 tttctttgat ttaaaagctt gcaaaaaaga gggagaatga gtattattaa aaatgtaggt
    18841 tagcactcga aggttaagag tgctaacaga ttaaaaaaag agttatcgga gggatttatc
    18901 gtgttaaaac cattaggtga tcgcgtcatt atcgaagtcg caaaagaaga agaaaaaact
    18961 gtcggaggaa tcgttttagc ttctgctgct aaagagaaac cccaaactgg gacagttgtt
    19021 gcagtcggtg aaggccgttt gttagaaaac ggtgaaaaag ttcctgccgc tgtaaaagca
    19081 ggcgaccaag ttatgttcga aaaatacgcc ggtacagaag taaaatacga aggaaaagaa
    19141 tatctgatcg tcgcaggaaa agatatcatg gcgattgttg agtaaatttg gaagataaaa
    19201 aaataatcaa aaataatcaa tgaggtgaat caatcatggc aaaagaattg aaatttgcag
    19261 aagacgcacg cgcagctatg ttacgcgggg tagataaatt agcagataca gtgaaagtaa
    19321 cattaggacc taaaggccga aatgtcgtac tagaaaaatc ttacggctct ccattgatca
    19381 caaatgatgg tgtgacgatc gcaaaagaaa tcgaattaga agatcatttt gaaaacatgg
    19441 gtgcaaaact tgtatcagaa gtagcatcaa aaacaaatga tattgcagga gacggtacaa
    19501 cgacagcaac tgttctgaca caagcaatcg ttcgcgaagg tttaaaaaac gtaactgcag
    19561 gtgctaatcc tctaggcatt cgtcgcggga tcgaattggc aacaaaagca gcagtcgaag
    19621 aattgcataa tatctcaact gttgttgatt caaaagaagc cattgcacaa gtagcagctg
    19681 tttcttcggg ttctgataaa gttggacatt tgattgctga tgcaatggaa aaagttggaa
    19741 acgacggtgt catcacaatc gaagaatcaa aagggatcga aacagaacta gatgttgtag
    19801 aaggtatgca gtttgatcgt ggttacttat cacaatatat ggtaacagat aacgacaaaa
    19861 tggaagctgt tctagaaaac ccatatatct tgatcacaga caaaaaaatc tctaacatcc
    19921 aagatattct gccattgtta gaacaaatct tgcaacaatc acgtccattg ttgattattg
    19981 ctgacgatgt agatggtgaa gcattgccaa ctcttgtatt gaacaaaatc cgtggaacat
    20041 tcaacgtagt agctgtaaaa gctcctggtt tcggcgatcg tcgtaaagca atgcttgaag
    20101 atatcgctat tttgacaggt ggtacagtaa ttactgacga tctaggttta gaattgaaag
    20161 atgcaacaat cgaaaactta ggaaatgctt ctaaagttgt tgtagataaa gacaatacaa
    20221 cgatcgtaga aggttctgga gaaaaagaag caatcgaagc tcgtgtgcaa ttgatcaaaa
    20281 accaaattgc tgaaacaact tctgattttg accgcgaaaa attacaagaa cgcttagcta
    20341 aattagcagg tggtgtagct gttgttaaag ttggtgcagc tactgaaaca gaattgaaag
    20401 aattaaaatt acgcattgaa gatgcattga atgctacacg tgccgcagta gaagaaggaa
    20461 tggtctcagg tggtggtaca gctctagtga acgttatcag caaagtttct gctgtagaag
    20521 cagaaggaga cgtggcaaca ggtatcaaga tcgttgttcg tgctttagaa gaaccaattc
    20581 gtcaaatcgc tgaaaacgct ggttatgaag gatcagtgat tgttgataaa ttgaagaacg
    20641 tggaactagg cactggtttc aatgctgcta ctggagaatg ggtaaacatg gttgaagcag
    20701 gtatcgtaga cccaactaaa gtaacacgtt ctgccttgca aaatgccgct tccgtttctg
    20761 ccttgttatt aacgacagaa gcagtcgttg cagataaacc agaaccagca gcgccagcag
    20821 ctcctgcaat ggatccatca atgatgggcg gtatgatgta agaaaaagca acttgcttat
    20881 ctacaactga tcattagtga aagacagata aaaagaggct ggaacagaag cgtttaactc
    20941 caagaaataa gaagaaattc acg
//
LOCUS       DACEPW010000031        20903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEPW010000031 DACEPW010000000
VERSION     DACEPW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.032"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..536)
                     /locus_tag="IW819_000921"
     CDS             complement(93..536)
                     /locus_tag="IW819_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1244041.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(754..2094)
                     /locus_tag="IW819_000922"
     CDS             complement(754..2094)
                     /locus_tag="IW819_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1244042.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2363..3259
                     /locus_tag="IW819_000923"
     CDS             2363..3259
                     /locus_tag="IW819_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1244043.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3409..4350)
                     /locus_tag="IW819_000924"
     CDS             complement(3409..4350)
                     /locus_tag="IW819_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1244044.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4476..5597
                     /locus_tag="IW819_000925"
     CDS             4476..5597
                     /locus_tag="IW819_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1244045.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5855..7357
                     /locus_tag="IW819_000926"
     CDS             5855..7357
                     /locus_tag="IW819_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1244046.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7424..7951
                     /locus_tag="IW819_000927"
     CDS             7424..7951
                     /locus_tag="IW819_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1244047.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8162..9223
                     /locus_tag="IW819_000928"
     CDS             8162..9223
                     /locus_tag="IW819_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1244048.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9226..10122
                     /gene="murQ"
                     /locus_tag="IW819_000929"
     CDS             9226..10122
                     /gene="murQ"
                     /locus_tag="IW819_000929"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1244049.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10136..12118
                     /locus_tag="IW819_000930"
     CDS             10136..12118
                     /locus_tag="IW819_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1244050.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12183..12629)
                     /gene="nrdI"
                     /locus_tag="IW819_000931"
     CDS             complement(12183..12629)
                     /gene="nrdI"
                     /locus_tag="IW819_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1244051.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12840..14264
                     /locus_tag="IW819_000932"
     CDS             12840..14264
                     /locus_tag="IW819_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1244052.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14445..15218)
                     /locus_tag="IW819_000933"
     CDS             complement(14445..15218)
                     /locus_tag="IW819_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1244053.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15215..16120)
                     /locus_tag="IW819_000934"
     CDS             complement(15215..16120)
                     /locus_tag="IW819_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1244054.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16274..16594)
                     /locus_tag="IW819_000935"
     CDS             complement(16274..16594)
                     /locus_tag="IW819_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1244055.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17068..17754
                     /locus_tag="IW819_000936"
     CDS             17068..17754
                     /locus_tag="IW819_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1244056.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17756..18700
                     /locus_tag="IW819_000937"
     CDS             17756..18700
                     /locus_tag="IW819_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1244057.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18899..19543
                     /gene="pcp"
                     /locus_tag="IW819_000938"
     CDS             18899..19543
                     /gene="pcp"
                     /locus_tag="IW819_000938"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1244058.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19622..20899
                     /locus_tag="IW819_000939"
     CDS             19622..20899
                     /locus_tag="IW819_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244059.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6753 a         3767 c         4344 g         6039 t
ORIGIN      
        1 cttattcacg gcgttccaga agtagcttct tcttatttct tgaagctgat aacttctgga
       61 acatcctctt tttttatttg gacggataat ggtcattcca tttctttgat catttttttc
      121 attttgtcga tctcttcttc ttgtgcctga atgatttctt ccgctaattt ttttacttca
      181 ggatctttta aatcagctcg tttacttgcc aggatagcga tagaatgatg aggaatcatg
      241 gccttcatcc aagctgtgtc gttgacagta gtttggcttc gtaccataaa caaggaaccg
      301 gcaaaaacaa gaagactagc ggctaagatc ataacattta tctttttgtt ttcatacatt
      361 ttccacatga acaacagcat gatgactgcc atgactgcac ccatcatcaa cgccatgaat
      421 actcgtgtac gactgaagaa gatatgatcg agcgcataga cattcaagta catcatacca
      481 aacataacta ccgttgaaac accgatcatc aacaaaaact tccaatactt tttcatttaa
      541 aacaaactcc tttttttcat tcctttcttc tatcatgtca tgagcatgga gaacaatcaa
      601 ttattttgca cagagggttt ttgaacaccg tttaccaaga ggtcatgaga ctgtttattc
      661 ggaaaaagga gttgcaacaa gacttttgtc acaactcctt tcttcaatag ctccatctat
      721 tccacataaa taaaataaaa ttttctatcg atttcattca ttcgtcaata cgaacgcatc
      781 attctttttt tgataactga aataaatgaa caggtaaagc aggccagtca atacagttac
      841 cccaatggag ttcgtgaaca ggataccaag gaaaatcaat cctaagacag ctaatgcaaa
      901 aaagctgatt cctttatccc ataatgttcc ttttcttagt gccagtacaa aggatgccgc
      961 actgataatg atccagatca ataatactgt atacgaaagg gaacctgcca gatagccaaa
     1021 taatttgtca ccgacaaaat aagaaagagc tacaccaaga taaagaacac tggtacaaaa
     1081 taaaacagca cgttgcggta cttgatgctt gtttaaaaca gctagttttc tagaggaacc
     1141 ttttcgatcc ttcaaacgga agaacaaaat tcttgaagat gcatacacac ccgaattgat
     1201 agatgagaac aaagcaagga caatcacaaa attgacgata tccgctgcaa atgggatatt
     1261 cattttttca aataccatca caaaaggact gacattcgtg cctgctaaag tattccaagg
     1321 aaaaagaatc aaaagcaaaa acaaaggaat gatgtaaaag gaaatgattc ttcccatgac
     1381 acctttgatt gctttaggaa tcgcacgttt aggatcatct gcttcgctta ccgtgatagc
     1441 aatcagttca gaaccgccgt aagaatagat aactacaagc aaagaactga tgattccctt
     1501 cataccatga ggagtaaacc caccatggtc agtcaattgc tgaagggtag ggaaaacgcc
     1561 tgtgcccaac atttgctgac cgacaagata agtagcaaaa atgatcagta agataatgac
     1621 actgatttta gcaaaagcca gccaatattc tgtttctgcg aataaccgta ccgaataaag
     1681 attgatgatc gtagttaaaa cagccaacat aaaaacaaat acccaggctg gaatgtgcgg
     1741 gaaccacaac tgcaaaaagg aagcagctgc aacagcttca gcgatgatat tgataatcca
     1801 catagaccag tagacccaat cagtaaaatc agcagaatga gcacctagat aagggcgtat
     1861 caaaccagat aatccgtgat gcgtgtcgcc accgcttaaa acgatttttc ccagaccttt
     1921 catcacgata tataaaatca gcccaccaat caaaaaagca atcaatacag aagggccggc
     1981 gatcccaatc gcttcgccac ttcctttaaa tagacctgca ccgattgctc cacctaaagc
     2041 taacatggtg atatgtctgg agctaagttg ttttttcaat tttttatctt ccataaatga
     2101 cctctttctt ttaaattcac aatcaaaatt tatattaaga ataaatagca atatttaaca
     2161 atgactaatg ataacaaatt ttcagacaat tacaactatt cagaaaactt ttaggcgaat
     2221 tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat tttcatacaa
     2281 atataggaaa cgttttactg gctttttact atttaaacag gctatcatag gaaaaagtga
     2341 cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg caaaaaataa
     2401 atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa aagatttact
     2461 acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc tgatcgtttt
     2521 tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa
     2581 aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc tgaagcaggc
     2641 acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag caaaagaact
     2701 tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt ctagcgaacc
     2761 aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag cagaaagcta
     2821 taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc cacatcgagg
     2881 gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag gggaatcgcg
     2941 cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga catttgaatc
     3001 catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt atcattttta
     3061 tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac cggaatttct
     3121 gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg cagaaaattg
     3181 ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat atgacctctc
     3241 ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg aaacaaacaa
     3301 gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta ttgggcttaa
     3361 aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt attctgcgcc
     3421 aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat
     3481 atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt cagtcaaacg
     3541 atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt tgtcaggatc
     3601 ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc cttcttttgc
     3661 tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg ctttttgcgc
     3721 gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg ttccatttac
     3781 acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat ttaaatttgc
     3841 ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt ccaagttctc
     3901 tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta gaccagctgt
     3961 ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca gtacatcttc
     4021 atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa tggattggat
     4081 cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca agtccacgtg
     4141 ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga ccacttcttt
     4201 accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag caggccaacg
     4261 atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc ggctgcccat
     4321 tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc aatgagttcc
     4381 cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa
     4441 aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg gcaattgatt
     4501 catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat ggctgggaag
     4561 atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat actgcagaag
     4621 caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat ctcttattcc
     4681 gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg
     4741 aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac
     4801 ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac
     4861 aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat
     4921 ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac
     4981 ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg
     5041 ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag
     5101 gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa
     5161 aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg
     5221 gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa
     5281 tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga
     5341 tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat
     5401 atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg
     5461 attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg
     5521 aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca
     5581 ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata
     5641 ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag
     5701 actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt
     5761 cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa
     5821 ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct
     5881 gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga
     5941 cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa
     6001 tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta
     6061 tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac
     6121 ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat
     6181 cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact
     6241 tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat
     6301 ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt
     6361 cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag
     6421 tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga
     6481 tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac
     6541 agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt
     6601 cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc
     6661 acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa
     6721 agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc
     6781 tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt
     6841 cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg
     6901 tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg
     6961 agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa
     7021 tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa
     7081 caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag
     7141 tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata
     7201 tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact
     7261 gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa
     7321 agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca
     7381 aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag
     7441 acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct
     7501 gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt
     7561 tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt
     7621 tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg
     7681 ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc
     7741 tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt
     7801 gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat
     7861 aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt
     7921 agataataca gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata
     7981 tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata
     8041 atataaactc aacctttaga aatgaaattt aattatttta ataaaagtat tgaaattttg
     8101 tttctttaga tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa
     8161 tatgtttggc ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat
     8221 ccgccaaatg gcatccaatg gctttgtcgg tattttcact tccatgcata taccagaaga
     8281 tgatgtttcg gcatatcaaa agcgattgat cgaactagga agcgaagcga aaaagaataa
     8341 actagaattg atggtcgata tttcaggaga ggcattggaa cgagcgggtt tttcattaga
     8401 agatctaacg cctttgaaag caattggtgt aacaggttta agaatggatt atcatatttc
     8461 caatcagcag attgccgact ggtcacatca attgaaaatc agtttgaatg ctagcacgat
     8521 cacgcctaaa gatatcgatg aattgaaaga agcagaagct gatttctcac agattgaagc
     8581 ttggcataat tattatccac gtcccgaaac agggctggac aaagaatggt atcagaaaaa
     8641 aaatcaatgg ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag gagatacaga
     8701 gttgagaggc ccactatatc aaggattgcc aactttggaa gaacatcgag ggatccatcc
     8761 tcttgcaggc gctctagatc tattagcaag taacacggat attgtttaca tcggagatgc
     8821 aggtctgtca gaaaacgttc aggagcaatt tgcttctttc caaaaagaac agacagtctt
     8881 gctccatacg gaacctgtag atgaagaatt ttacgaatat atattaggga agcatacgaa
     8941 tcggcaagac gatgcaagag atgtcatacg aagcgtagat gcacgttttc gagagattcc
     9001 gcctatccca gcaagaaata cagctacgcg tatgaaaggg tctattactt tagataacga
     9061 aaagtatttg cgctatatgg gtgagatcca attaaccaaa tatgatttgc cagcggatga
     9121 aaaagtgaat gtagtagcta aagttatcaa agaagagcta ccattgatca accaaatcaa
     9181 agctggtatg aactatcaat ttataagaaa agaaggaaga taaaaatgag caaaatcaat
     9241 ttagagaacc taacaacgga acgcagaaac caagaaactt ttggattaga tgagatgagt
     9301 atttctcaag ccgttcaact aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa
     9361 aaagaactga caagtatcga accagtgatt gctaacacga ttgagtcctt taaaaaaggt
     9421 ggacgtctga tttatatggg agccggaaca agtggaagac taggtgtgtt agatgctgct
     9481 gaatgcgtac cgacattcgg tgtagaaccg gaaatggtca taggtctaat tgctggtgga
     9541 gaagaagcaa tgactgtagc tgtcgaaggg gccgaagatt cttatgagct agggcaagaa
     9601 gatctaatca agctgaattt gacaaaaaat gatatagtta ttggtatcgc agctagcggt
     9661 cgtacaccat atgtgatcgg tggattagat tacgcaaaat cgatcggtgc tgtcacagga
     9721 acgatttctt gcaataaaca agccgaaatc agtaaacacg cggatttccc aatcgaagtc
     9781 gatgcaggtc cagaattttt gacaggctct actcgattga agtcaggaac agcacaaaaa
     9841 cttatcttga atatgatttc tactatcagt atgattggta taggtaaagt attcaataac
     9901 ttgatggtcg atgtcaaacc aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc
     9961 atgcaagcga cagaagctga ttacgaaaca gctgaaaagt actttgaaga agcagatcaa
    10021 aatgttaagc tggctatcgt catgattttg acaaatagtg ataaagagga agctcaagca
    10081 aaattagctg aagcaaacgg atttgtaaaa gggaccttat gagggggaaa agaccatggc
    10141 aaaaaacgaa ctgactttgg aagaactggc aaaacaaatt tataaagggg caggcggcat
    10201 gggcaatgta gaaagcattg tccactgcat gactcgtgtg cgtatgaaga tcatcaatga
    10261 tgatcttgta aatgtctctg aattaaaagc gattccagga gtgcttggtg tagtagaaga
    10321 tgaacagctt caagtcatca tcggacctgg gaaagtgaac aaagtagcac agcaaatggt
    10381 tgatttggca ggcgtgaaat taggagaaaa actttcttct gtaagtacag ctacagcaaa
    10441 ttcatcagca tctggaaaag accgtgtaaa cgaacgggca caagaaatga aagcggcaca
    10501 aaaatcgaaa caaaaaccat ctaaaatcaa atcggtatta aaagacattt ctaatatctt
    10561 tgtgccaatg attcctgcat ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt
    10621 agcaaactta gtcacagccg gaacacttga tgcagcaaca tggcaacaat atatcgatgt
    10681 gatgaacatc ttgaaaaacg ggatgtttag ctacctagtt atctatacag gtatcaatgc
    10741 tgcacaagtt tttggtgcaa caccaactct tggtggagtt atcggggccg ttgtgatgct
    10801 gacagggatg aatccggaag caccactgaa aaacttattt actggtgaag ctcttgcagc
    10861 tggacaaggt gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa
    10921 aaaactgcat aaagtagtac ctgatgcggt agatatcatc gttacaccaa ctctttcatt
    10981 gattgccatc ggtctgattg aaattttctt catcatgcct ttagcaggtt tcatttctga
    11041 tggcatggtc ggtgggatca actgggtact tggcgttggt ggagcatttg ccggcttcgt
    11101 tctaggcaca ttattcttgc cgatggttat gttcggactg catcaaattt tgactccaat
    11161 ccatgtgcaa atgattgatg aaactggtcg tacgctattg cttccaattc ttgcgatggc
    11221 tggtgcagga caagtgggtg cagcattggc attatggatt cgctgcaaaa aagataaaga
    11281 attaacagaa atgatcaaag gagctttacc agtcgggatc ctaggaatcg gcgaaccatt
    11341 gatttacgga gtaacattac ctctaggacg cccatttatt actgcttgta tcggtggagg
    11401 tatcggcggg gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc
    11461 aggagccgct ttgatcccat tgatctctga cggtaaatgg ttaggctaca tccttggatt
    11521 attggcagct tatgctggtg gcttcgttgc tacattcttc ttcggtattc ctaaagaccg
    11581 tttagaaaaa gaagaattag cgactgaaac agccacagta aatgaagtgc ctgaaaaagt
    11641 agtacctcaa acttcagctg aaagtgtgac tatcctctca tcagttgctg acggaacagt
    11701 tgtttcttta gataaagcaa gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta
    11761 ctttgtagaa ccagaaaacg gacatatcta ttctccagtt actggggtag tcagctctgt
    11821 cttcccaacg aaacacgcta ttgggatcac aacgccaaat ggtttagaaa tcttgcttca
    11881 tatgggaatc aacacagttg acttaggcgg tacgccattt gatgtgaaag taacagaagg
    11941 gcagcaagtg acacctgata cgcttgtagc agaagctgac ttagaagcga tcaaagcagc
    12001 aggcaaagaa acatccatga tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt
    12061 cttggaaaaa acaggtaaag caaaagcaaa agcacctgtg atggatgtca aagcttaata
    12121 tgatagaaac aatagaaacg aaagaagaaa ctgaaatcta tttttcagct tcttcttttt
    12181 tattatttat tgttttctgc taaaatctga taaattcgtg tcacatcaga tggtgttccc
    12241 cgtaattcat aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt
    12301 agacaatact gattattgaa gtttttattt ccgctaccga ctacaccaag acaaagttta
    12361 tgattgtctt catgttccag atattcacgc atcgtttcgg tcaagatttc tgtatcaccg
    12421 ttatctaaac catttccgcc gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt
    12481 tctttcgtaa aatcactatt ttcatggatt tcttttaaag taacttcagg attgacagga
    12541 tcttctgcat gcattttttc agcatattct gctagatgtt tggcaaaggc acgcgtattt
    12601 cctgaaatcg atatatataa aatgttcatg gatcatcctc caattctttc cttttagtat
    12661 agcatctttt ttaagttttt tggtggtgta atttggaaag ggactaggcg ctttgcttgt
    12721 ataatgagaa aaaagccggt agaattatat ttctattaaa gtttttatta attaagtaaa
    12781 aataaaaaag gtttgttata ctttttgata aaagcggttt tattttggag gatgcttaca
    12841 tggatacaga gcaacagaaa aaaggaattg gtttaattcc acttgctgcc ttagtaattg
    12901 gttcagcgat tggaggtggc gtatttggta ttatgacgga catctccgga tcggctggcg
    12961 gcccagctat tttcagttgg gtactcgtag gaacagcaat gttgatgctc tccttgtcga
    13021 tcaataatat caataaaaaa cgtccagatt taaaaggcgg gatctttagt tacgcagaag
    13081 aaggttttgg ccgtttcgct ggttttatca gcggttgggg ttactggctc acatgctggt
    13141 taggcaacgt agcttttgca acattgttga tgagcgcatt aggatatttc ttcccagtat
    13201 ttgaaggtgg gcaaaatctg gcatcgatcg tgatgagtac agtcttttta tggggatatg
    13261 catggctagt gaatcgggga gtcgaaaatg ctagtttcgt caatgctatc gtcacattct
    13321 gtaaattagt gccattattt ttatttattg tagtagctat cagcacgttt tcgatccaac
    13381 aattcattga taacttttct agcgacatta tattgaatgc gaatggacag ccgatcccgt
    13441 tttccagtca agtattgagt tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag
    13501 gagcctctgt cgtaggttca cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc
    13561 tagggatatt cggcttgatt attatttacg tcttggtttc catgcttcca tatgggacaa
    13621 tgacgatgaa tcagctgcaa acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa
    13681 tgatggttgg taaatgggga gcagtcgtga tcaacggcgg tttgatcatt tctttagttg
    13741 gtgtttggct ctcatggacg atcttaccga ttgaaacaat gggaaacatg gctgaagacg
    13801 gacttttgcc taaagcatgg ggaaaactca acaaaaatgg ggcaccaact tatgctatcg
    13861 tattgacgac gttatgtacg aatgtctttt tactttcatt attattcact gacagtgctt
    13921 ataactttgc ttacacactt ggtacagcag ctattttctt cacgtggctt ttcttaggat
    13981 tgtaccaaat gaaactttct tatcagcgga aagaatgggt ccaattttta gtagggcttt
    14041 tagcaagtgc ctttcaagta tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg
    14101 ttcttgttct tttcttgcca ggcatttatt tttacctcaa agcacgtaag gagcaaaacg
    14161 agcctgtaca acatatccac ttagataaga ttttacttat aggtgtgacg atggtcggag
    14221 tgattgcaat ggttttattt gccactggga tgcttcacgt atagaagtat aaaagaagta
    14281 taaagagagg ttgtgagcct gtcgtttatt caaaaacata ctaggaaagg agctgtgaca
    14341 caaatgttgt cacagctcct ttttcagaat aaacggtgtt taagagacag ccaatcggca
    14401 ataaggctga acgtctcttt cacagcctct tttttgatga gtcatcatac attttttcgc
    14461 ttaaatacga gatatcctaa tgctaagaac aaaacgatgt aagctaaatt accgaatagc
    14521 atttgattag tagaaagatg agtcaattgc ttcatgatct cttcgtttcc aatttggttt
    14581 tgcagattca acatactgat cggattccat ttgatccact cccatttttg gatgagtgca
    14641 ataaggatac cagatatgat tgacgaagca aaataaaaga cgattcctgc tgagatggat
    14701 gcgccggaac tattgataaa gcaagcaatc atcaacacta aacttaaaat cagccataaa
    14761 ccgatataac tacccaaagt ataagtgaac aagctttgga tcagtgtttg accagtagaa
    14821 accttctccg tgaaggaaat cgaagggaaa agaatcagtt tcatcaacac agtgacaata
    14881 atagctaaaa ggtataaata aacagaataa ataactaatg tgatccattt gcttactaat
    14941 atttctcctc gagaatactt tcgataaagg agatttttca atgtaccata ttgtgcttcc
    15001 atcgaaatga tcgtgcttgc agctgcaatc atgataaaga caatccaaga tgtggcacta
    15061 aaaagctgag cagtcatttc aactggatca atgatcgtcg tgtatttttt ggctagaagt
    15121 gctgttccta tgagcaaaac aactaataga acagataaaa cagcagttga ctttttcttg
    15181 atcaatttaa ataattcttg ttttacaagc aatgtcattt ttcttcttcc ccctttactg
    15241 aatccagaat ttccaataga gaagattcca aatcattcgt cagatgctgg acgtctgtca
    15301 catggatatt ttgtgcagac aatagctgga tcacggttcc taataattct tcgttttctt
    15361 ttaaaagctt gattttatca ggatcttcca aagtatatcc agcttctgtc aagatcgttc
    15421 gagcagctag atcatttgtt gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt
    15481 cagccattgt acattgtttg atgatctttc cttgattgat caggatcagc tcatcgacca
    15541 ttttttcaag ctcgctgagt aagtgactgg agatcaagaa agaggtgccc tgagtagcca
    15601 agtcggtaat gatttttcgc acatctcttg ttgcttgtgg atccaaaccg ttcatcggct
    15661 catctaaaat aacgatagac ggatggttca gtaaggcaag agcaattccc aatttttgtt
    15721 tcattcccaa cgagtaggat ttcgcttttt tgtgaatgta cttttccatc ttgagctgat
    15781 tcacgatagt gtcgatagca gatacatcgt tggtttcaga gaataacttc agatgatcat
    15841 agcctgttaa gaatggatag atacccgggt attcgatcaa tgctcctact tgttccaacc
    15901 ctttatgact agagggagaa atctcatttt caccaataca gattgttcca tctgagtaat
    15961 ggatcagtcc taaaatagct ttcatgatcg ttgtttttcc agcgccatta ggacctacta
    16021 atccaacgat tttgccaggg gataagctaa aactgacatc gctgagggct tgtttgtgac
    16081 caaagttctt ggataattct tggaccgaaa gacttttcaa aaatattcac tccttttcta
    16141 tagatagtac aagcatacac ctgatgagtg gagaagtcgc taggtcattg gacttatttc
    16201 ttattttttc ataaaaaaat tgaatattaa ataaaaaggc ttattttttc tgatcagtac
    16261 ttattccaag tgtctagtgg ccgctagtaa tcttgatgcc aatgatactg atgagtagca
    16321 tccccacaaa gaaccaagtg gaaagattca gcttatcatg aaacaagacc acaccaatta
    16381 tgaccgaacc aactgcgcca atccctgtcc agataggata agagagactc atcggcaaac
    16441 ttttgattgc ctttgacaaa aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag
    16501 aataattgat tttagaaaaa ccatcactcc atttcatcat cgttgaccag aatacttcca
    16561 aaattcctgc tatgattaat tccatccatg ccatacttac attcctcctt aactataaaa
    16621 aaatacacaa aaagaggaaa atcatccctt caaagaccta atggaatgat catcctcttt
    16681 atcctacccg gtggcagatt gtatatcttt ttctgtagta tagcacacct atccagatat
    16741 cggcaatctc atacttttta gtaaagtgta ccaagcactg acggatatat cgttagcgga
    16801 aaagaagatg aatgctttgc aatttcatag aaagaagttg ttatttatta atcatttaaa
    16861 aaaaacagta actagacctt atttctatat attatcgggc aataaattaa aaaaagcttt
    16921 accttacaat aagtaagata atttttgtga taaaaaaata acgaaaaata tttaaaaata
    16981 gtgtctattt cgtgagaaaa caactatatt ttttatgtgt tttgctttat aattaaaaaa
    17041 atattgtcat tttatggagg aaaatatatg gaaaattatc ttagcctttt aggcgtcttg
    17101 attgttattc tcgggtttgc atttaaatta gactcgatat tgatcgtaat ggttgcttta
    17161 gttgttacag ctttgactgg cggtcttggc attcaaggaa tgttagaact tttaggtaca
    17221 agttttgtaa acaatcgcgg gatggcgatt tttatcatca ttatgttggc aacaggaacg
    17281 cttgaaagaa atggtctgaa agaatcagcg gcttccttga tcaagcgatt caagaaagta
    17341 tctgctggta tgattgtaga tatttatggg atctttcgaa tgatctttgc ggcttttaac
    17401 gtgagttttg gtggagttgc tggatttgtt cgtccaatca ttttaccgat ggcattgggg
    17461 accatcgaat caaaagggtt agaagtccat cctaagcatg aagaagaact aaaaggtatg
    17521 gcttcagcta tggaaaatgt ctgctggttc tttggccaag tactattcat cggaggagca
    17581 ggggcgttgc tagtccaatc aacactgaaa gatctaggat atgacgtaac attgggacag
    17641 cttgctttag tggaagttcc agtggctatc acggctttag ttgtggcaag tgtctacttc
    17701 tatcttaaag aaaagaaatt agcgaagaaa tattatccag gaaaggggca ataagatgga
    17761 cttttttaca aatcctgata ttttattagg cgataaatta ctggagatcc tttatatcgt
    17821 gatgggactc gtattgattt acactggagt aagaaattta acagataaaa ccaatcctca
    17881 tcgttatggg tcagcctttt tctggacggt cttaggggta gttattgcag gaggaagatg
    17941 gcttccggca atcgtgaacg gtggcttgat ctttgcgatg accataccag caattgctaa
    18001 gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga
    18061 caaactagga atgaaaattt ttattccagc tttgagtatc ggtgtctttg cgatcttgtt
    18121 tgctctattt accaatttag gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat
    18181 gctaatcttg atgttctttt ctcgagataa tcgaccaagt acatttttag atgatgcagc
    18241 agatatgctg ggcacagtag ggccgttaag catgttgcca atgcttttag cttctctagg
    18301 tgcggtattt acaagcgctg gagttgggac agtgatttcc aatgcagtag gtacgatcgt
    18361 tccagaagga aatgtgaaca tcggcatcat catttacgct cttggcatgg tgatatttac
    18421 ggtaatcatg gggaatgcat ttgcggccat caccgtgatg acggttggga tcggtgcacc
    18481 attcgtattt agtcatggag cgaatccagc gttaatagga atggttgcat tgacttgcgg
    18541 tttttgtggg accctgctga caccgatggc tgctaacttc aatatcgtgc ctgtagcgat
    18601 gctggagatg aaagacaaat acggagtgat caaaaaccag ttgtttattg cgttatttat
    18661 gctagttttc caaattgctt atatgattct atttaaataa tagaagagag gggttgtgac
    18721 agaataattg tcgcaacctc ttctaacatt gtagtggaac actatttttt tataagaata
    18781 tattaagttt gatctgttat gttatttaaa ctattgtttc ctttatttac gctgttcaag
    18841 gaaaatggta tgataaaaga taaccatatg ttaaatcaat cggacggagg aacaacagat
    18901 gaaagtacta gtaacaggtt ttgatccatt tggtggggac aaagtgaatc cagcctatga
    18961 agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa atcatcaaag tggaagtacc
    19021 gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc atccaacagc accaaccaga
    19081 tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc
    19141 aatcaatcta gcagaagcaa gaatccctga taatgaagga aatcagccat tcgatacggc
    19201 tttagaagaa aacggtccag ccgcttactt tacctcattg ccaatcaaag caatgacaaa
    19261 aaatgtacaa gatcatggtc taccagcgta catctcttat acagcaggaa catttgtctg
    19321 caatgatatc atgtatcgtt tgcttcattt gatcgaaaca caattcccgg caattcgagg
    19381 tgggttcatc cacgtgccat tctcacccga tcaagtgatc gatcgtccag tgggtacgcc
    19441 ttctatgtct ttagaagaca ttgcttcttc tttgacttat gcagttgaag cagcagtaga
    19501 aaataaagaa gatatttccg gaaatgccgg aacaactcac taatttagca agaaacactg
    19561 gctggttgct aacgttatgt tagtagccag ctttttgtaa ttagaaagga aagaaacagc
    19621 cgtggaagaa tggcaatccg tctttgaaga atggtttcca aaagaaatca gcaaaagcta
    19681 tcccatcaaa atttctaaac aatacacaag cagtcaacgt tgggagatct atgcaaaatt
    19741 aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga tatctaatca gctctcgttt
    19801 tatggaagaa cattatttag ctgcaacaga ttgggtgttt tctgacttca agatcaatcc
    19861 gttttttcga acaaaacgaa gccagcaaaa attgtattgt gagtgtggta gagaattaaa
    19921 agtgcaatac atcgttaaat cgccaaaaac cggaaagatc ctaaaactag gcatcaacca
    19981 ctttgccgat cacctgcatg tctctccgac agtggcagct tcgatccatc agggaatgac
    20041 aaaagttgat ctggccttag acgaattatt atggcttaag cagaagaata tcgattttcc
    20101 tgaaggacta tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc
    20161 atatttaccg gatataaaat tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc
    20221 gatttacata gcggattatc aagcattgga aaatgaaatc aagaaaataa gtgaacacat
    20281 caatgggcag tcaaaaaagc gacagataaa aaaagaacta tttgacgact ttgcagaaga
    20341 attagtaaag gatgtagaag aattcttgat caactatcgc gcatttttac gtaaagattg
    20401 gcaatccatc gtatatgaag aagtgccagt acatcctaat gcttattttg aaacatttat
    20461 ttccgtgttg agaaagacaa aacgtcaacg aacaccagaa gtcactgctc agatggaata
    20521 ttttgcgaaa aaccaacgat tcatccaacc aaaaatctat ctatttattt ggaagcaata
    20581 ttgtcgctat ggttttacag aagggttctt cgacagcatt cctcggatag tcagaaatgg
    20641 ttttttaaaa gtattgcgta aagaacgaga agctatacaa tctgctgata agaaagaccg
    20701 tacagtctca aaagaaaaat ggcaactagt agtgaaagat atccaatctg gaaatgtgca
    20761 agagacaatc gacaaatgga aaggaaaaca ttatcgtttt actgaagctc agaaacaagc
    20821 gttggaatat tatcagaagt tagaagaatc actgcgtttc aatgatgagg caagaaagta
    20881 tctgaaggaa ttactataaa aaa
//
LOCUS       DACEPW010000032        20540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEPW010000032 DACEPW010000000
VERSION     DACEPW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.033"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..442)
                     /locus_tag="IW819_000940"
     CDS             complement(<1..442)
                     /locus_tag="IW819_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1244060.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKY"
     gene            complement(572..1282)
                     /gene="budA"
                     /locus_tag="IW819_000941"
     CDS             complement(572..1282)
                     /gene="budA"
                     /locus_tag="IW819_000941"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1244061.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1295..2959)
                     /gene="alsS"
                     /locus_tag="IW819_000942"
     CDS             complement(1295..2959)
                     /gene="alsS"
                     /locus_tag="IW819_000942"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1244062.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3164..3826)
                     /locus_tag="IW819_000943"
     CDS             complement(3164..3826)
                     /locus_tag="IW819_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1244063.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3836..4651)
                     /locus_tag="IW819_000944"
     CDS             complement(3836..4651)
                     /locus_tag="IW819_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1244064.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4913..5356)
                     /locus_tag="IW819_000945"
     CDS             complement(4913..5356)
                     /locus_tag="IW819_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1244065.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5490..5828)
                     /locus_tag="IW819_000946"
     CDS             complement(5490..5828)
                     /locus_tag="IW819_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1244066.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5816..6193)
                     /locus_tag="IW819_000947"
     CDS             complement(5816..6193)
                     /locus_tag="IW819_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1244067.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6417..7610
                     /locus_tag="IW819_000948"
     CDS             6417..7610
                     /locus_tag="IW819_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1244068.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7776..8198)
                     /locus_tag="IW819_000949"
     CDS             complement(7776..8198)
                     /locus_tag="IW819_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1244069.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8593..8787
                     /locus_tag="IW819_000950"
     CDS             8593..8787
                     /locus_tag="IW819_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244070.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8784..9278
                     /locus_tag="IW819_000951"
     CDS             8784..9278
                     /locus_tag="IW819_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244071.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9440..10612)
                     /locus_tag="IW819_000952"
     CDS             complement(9440..10612)
                     /locus_tag="IW819_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1244072.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10818..11633)
                     /locus_tag="IW819_000953"
     CDS             complement(10818..11633)
                     /locus_tag="IW819_000953"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1244073.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11783..12229)
                     /locus_tag="IW819_000954"
     CDS             complement(11783..12229)
                     /locus_tag="IW819_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1244074.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12226..13230)
                     /locus_tag="IW819_000955"
     CDS             complement(12226..13230)
                     /locus_tag="IW819_000955"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1244075.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13270..13599)
                     /locus_tag="IW819_000956"
     CDS             complement(13270..13599)
                     /locus_tag="IW819_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1244076.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13725..16052)
                     /locus_tag="IW819_000957"
     CDS             complement(13725..16052)
                     /locus_tag="IW819_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1244077.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(16895..17188)
                     /locus_tag="IW819_000958"
     CDS             complement(16895..17188)
                     /locus_tag="IW819_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1244078.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(17591..18769)
                     /locus_tag="IW819_000959"
     CDS             complement(17591..18769)
                     /locus_tag="IW819_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1244079.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(18873..19187)
                     /locus_tag="IW819_000960"
     CDS             complement(18873..19187)
                     /locus_tag="IW819_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1244080.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            19294..19509
                     /locus_tag="IW819_000961"
     CDS             19294..19509
                     /locus_tag="IW819_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244081.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(19809..20051)
                     /locus_tag="IW819_000962"
     CDS             complement(19809..20051)
                     /locus_tag="IW819_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1244082.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(20038..20466)
                     /locus_tag="IW819_000963"
     CDS             complement(20038..20466)
                     /locus_tag="IW819_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1244083.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         5993 a         4069 c         3523 g         6955 t
ORIGIN      
        1 catatttttc tagaaatgct tgatcgattt catcataccc tttcgttact ccgcaatcga
       61 tatctaaaac accgattaaa ttaccttctt ttaccattgg cactacgatt tctgattgtg
      121 cggctgaatc gcaggaaatg taatttgcat gggttttgac atcatcaacg atcaatgttt
      181 cttgtttttc agctgattct ccacaaactc cttttcccat cttgatacgt gtgcaagaaa
      241 ctcgcccttg gaacggtcct aaaatcaatt cattaccatc gaataaataa tatcctgcaa
      301 agacagtctc aggcaaagtc tcattcaaca atgcagatga atttgctaaa tttgctaacc
      361 agttatcttc tgcttctaat aatccttgtt gctgtaataa taaaatttcg tatgcatctt
      421 ttttctgttt ttctgtccac atgataaaaa ccgctccttt ttgatctatg gagaagatta
      481 tagctcagtt tttttgtaaa cgctaggttt cctcctcaaa aaaacgagat acgtcaggtt
      541 aatcaccttg aacgcatctc gctgtctttt tctattctgc ttgttggatt tctgctgcta
      601 aatcagcata atcaatcgtt tcgtgaacaa aagtcggatc atcaactgga aaatgctgta
      661 caagggacga gatgcggcta atctcaactt ttccttcagc cacttcaaaa tccattacat
      721 gtccgccaaa tgtatgtgta tcatcgataa aatgcaagtg aaatcccgct gcagcaactc
      781 cttggaataa ttcaggcgta aagaacccga ctaatgttcc ttggacattg ctttgttgga
      841 attctggttg agactctgaa atttttgcta gtcttctgta aggtttttct tgcttcggca
      901 tgatacggat gtgcatattt ttgaataatc cagttatctt cactgccaaa aataggtttt
      961 ccccggcttc tcttactaat atgtcttttt tcaattgttc tccatctgca gtttttgaga
     1021 cagaaaaact agtatctggc gtaaaatctg taattgctgc atagggggta agttcacttc
     1081 catctaattg ggcaactgag ccatctgatc ttccttgata agctacgcca tctaaaaaga
     1141 tgacttcacc atcaagccca tgcaatgttc ccaatcccag agttcctttt tctaatatat
     1201 gagcaatcgt ttctgttcca tccatcaagc cagccatcaa agctcctaat gtcccgtgct
     1261 ggtataagat tttttcagtc atgtcgacac ttccttaata gaattgatct ggtaagattg
     1321 tttctcccaa tttctcatta tcacggtaat caatcggaat atcaataatt acgggtcctt
     1381 ctgtcgcaaa tccttcttct aaagcttttt ctaattcctc tacacttgta gctcggattc
     1441 ctttagcgcc aaaagcttct gcatatttca caaaatcaac tggaccaaag tctacacctg
     1501 acgcccgctg atatttcatt ttttcttgga actctaccat attgtagtgt ccatcattcc
     1561 aaatcaaatg aacgatattc aattttttac gtaccgccgt ttctaaatcc tgggcagaga
     1621 ataaaaatcc cccatcgccg gaaactgata cgatttgtgt atttggacgc accaatgcag
     1681 cagaaatcgc ccatggcaat gcaacaccaa gcgtttgcat accattactg aataacaaat
     1741 gacgaggttc ataacttcta aagtggcgtg ccatccaaat ataatgacta cctacatcta
     1801 cggtaacagt catatcatct gttacttttg actgaagcgt gttaataact tctaaaggat
     1861 gcaagatacc tgcttctcct tttgattgaa caatgtcccg ctccgttaat tctgcttgaa
     1921 gagatgctaa atattctttt gcatcttctg acacttgttg aggctctaat gaacctgtca
     1981 ataaatcaag agtggctgag atatcaccga tcaattctct ttcaggctgc ataaaaggat
     2041 cgatttcagc tggcgcttca tcgatcacaa tgatccgcgc atctttttcc gcattccagt
     2101 ttcttgcttc atattcgatt ggatcatagc caatagcaat aactagatca cttcgtttca
     2161 aaagcatatc tcctggttgg tttctaaata aacccacacg accaaagaaa tgcgcttcta
     2221 attcacgtga gatcactcca gctgcttgga atgtctctac aacaggcaat gctgtctttc
     2281 caactaattg cctgatggct aacgtttctt tttcacttga tcctcgcata cctactaata
     2341 gtaccggtag ttttgcttct ctaatgcgtt cagctaggta acggatatca tctgcagaag
     2401 cagaacctaa tttaggatcg ctcaatggct tgatgacatt cccttggaca ggtgcatcca
     2461 ctacatcttg cgggatactg ataaagctcg cgcctttttt tgaactctta gccatacgat
     2521 aagcattggc aatcacttct gaaattgttt ctggatcttg gatttctgca ctgtattttg
     2581 tgattggctg gaacagtgct gcattatcca tgctttgatg ggtcaacttc aatagatcgc
     2641 ttcgttttac ttgtccggca attgctaaaa ctgggtcacc ttctgctgtc gctgttacca
     2701 aacctgtagc taagttacta gcaccaggac cacttgttgc gataactact cctggctctc
     2761 ctgtaatacg tccgattgct tgtgccataa atgcagcatt ttgttcgtga cgagtcacga
     2821 ttaattcagg accttgatcc tctaattcat taaatacacc atcgattttt gcacctggta
     2881 taccgaatac ataatcaaca tgatgattaa tcaaactttc tactacagat ttcgaacctt
     2941 gtttcatttt ttcgtccatt gaacatcgtc ctttttatgc tttattttta attattagtt
     3001 agaaataatc acaaataatt gatacagttt ctaaacctgt ttcattcaaa agtatagcac
     3061 gttttatttc atttgtttag ccatatgttt caaaaaatga aaaagaatga cagtttttac
     3121 ataaaaactg tcattcttaa ttgaaagttt caatttataa agtctacaga tccatagttc
     3181 tcatcaattt acgattgttg aagtaaatca aaccaaatac gatagaagga cctagtagaa
     3241 tacaccattg gggctttacg aaaaagaaga gaaacaaact tgtcaatagt aaaaaatcta
     3301 gtaaactcac ataccatttt ttcaacgtat agtagaacgc tatccgtaga acttgatttg
     3361 gtttcgacgc tggatttctc acctgaaaat accagtagtt gatgctaaag gaaactgata
     3421 aacaagcaag taaaacaaac aacggaatca accattttcc ataagaaaaa cgtataaaaa
     3481 agaaacaatc aaaaatcatt atcacactaa ctatccaagc aaatagccaa taaaaaaatc
     3541 cctttagcca aaattggcga aacgtcttta tgaacaaacg tactggtgaa aaactttttt
     3601 cttttgctaa ttcttctgtc atacctatca tcacgatgca acctggacca ataaaaagta
     3661 aagaaaaaat aaaggaaaag acattccttc catctatcga caaaaggaac gcagctgcta
     3721 tatacaagat attgacacac caaaaacaaa ggctacttgc caatgattga tacaaccaat
     3781 aaaagaattc catataaaca tttttgtcaa aacgctgctc tttcataatc acctcctatt
     3841 ccttcattcc tgatgaagcg atcccttcta caaaatattt ttgcatcgct aaaaatataa
     3901 aagcaacagg aagaacagaa acaactgagg cagccatgat taatgcgtac tctgcatcaa
     3961 atagcccaat aaacattttc aaccctaact ggattgtttt attttccgta gaagacaaat
     4021 aaatgaatgg ccccatataa tcgttccatg tattaacgaa tgtgatgatc gtcaaactgg
     4081 caatcgccgg ttttgtaaga ggtaaaatga tcttgcgata gattccccac tctgataagc
     4141 catcgatccg cgcactctca cataaagaat ctgggatact actgtagtat tgcttcaaca
     4201 agaacacacc aaaagcattg aacgcttgta atagcaccaa cgaccacaac gtatatgtta
     4261 aacccacttt tctcatcata atgaactgcg ggatcatata agactgccaa gggacagcaa
     4321 tcgtgccgat atatgctaaa aataaaatat cgcgcccttt aaaatttagt ttagaaaacc
     4381 cgtaagcagc aaaactagaa gtaaacaact gaatcaacgt gatcacaacg cttaaaaaag
     4441 ctgtattctt aaaaaatgtc actaaaggaa tacgagacca aataaccaga taattctcta
     4501 aatgccaagt tttagggatc cattgaatcg gaatcgtgaa cacttcatta tttgctttga
     4561 atgaagcaga aaccatccat aaaaaaggaa tgatcgtcaa taaagctaat acaatcagca
     4621 gaatcatacc caaccattga ataaagggca tgttttttga acgaattttt gctttttttc
     4681 tttcatttcg aacacctcca aagttataat cttcatttct ttgtaatgat tacaatccag
     4741 aacaacggtt attatagctg attcatttcc gttcttctta tgtttgtaag cgcttattta
     4801 acaagttatg taaatctctt tatttttttt gatttaaaaa tgaaaaaata caaaagcaag
     4861 actgtcatac gtctcgcttt tgtattgaaa tttcttcgat tagagatact tattactgtt
     4921 caaaagcttc tttgaaacgg tgaatcaatt cattgactac aggtgataaa atccgattct
     4981 ttttccagac tagcatgcag ttcgtttcaa tcattgggtc aagcggaacg aatgtcactc
     5041 gctttgaaag atgacttaca gcaccttcta tgactaaagc agcacctacc tgattctcaa
     5101 ttagcgcaaa tacattgaaa atcaaattgt acgttcctgt gatcagcaac tgctcctttt
     5161 ttatgcctaa ccagtcagca aacattcgct gtatttctgg acgctttgag atcaaaagag
     5221 gtacattcgc aaagtcttct tttgttatca cgtctttttt tgctaaaaca gaattttcta
     5281 aaatgagcaa tccccatttt tcctttcttg gtaacaaaat cgtttcgtat ttctctgtat
     5341 ctactggatc tagcaaaatc gctaaatcta acaggctttt ttctagtcac tccgtgatat
     5401 catcacttgt tccgctgtgg atatgaaatt gaacgctggg ataatcacgg atcaactttt
     5461 ctagtaatag tgccaatgta ttcgaattat cagcttccac acagccgatc gtaaatcggc
     5521 cgctgaataa ttgttttttt ctgttctcga attcttgagt ggtttgattc gttaaatcta
     5581 aaatttcttc tgctcggctt ttttagaaaa taacctgctt ctgtcaagat cattttgcgg
     5641 ttttcgcgta caaacagttc tgttcctagt tcctcttcta actccttcag ttgtcggctt
     5701 aaggttggtt gtgtaagatg tagaacttct gcggcttgag agattgttcc ttcttctgca
     5761 attgtccaaa aatattttag taagcgtaac tccatagcga tgctccttga tttttttact
     5821 tgatacattc cattttattt ctagataaca cacgatcata tttcttgatt tttccttgta
     5881 aacgttccaa acactcttgc atttcagcaa tttctttcgt caaaagttca ctttcttcaa
     5941 tcaaaagctt acgccgctca aatacagttg cttctccttc gctagatagg cgaatatatt
     6001 caatcaaaga ctcgatcgat aatccggcat ttcgcataca gatcgtaaag tcgacccatt
     6061 ttaggtcttc ttctgtataa tcacggattc cgccttttgt ccgattaact ggtggaatca
     6121 acccgattcg ttcataatag cgcaatgtat cggccgaaat gcccttttct tcactgactt
     6181 gtttgatatt catctgatca ctctttctct taatttcttg ccactttact tatgtatttt
     6241 atttattcta caactttaag tgtactccaa gtcaatactc atttctatta aaactaagct
     6301 ggattatcta tatcggttgt ttgtgtatta tttcaaataa aaaagtcttg tttttttatt
     6361 cttttttgga tatactttta acaatttagg gaaattttag gaaaaggggt ataaaaatga
     6421 caacgtatca gcgaaaggta ttattatcga catcggcggg aattgcttta gagaatatgg
     6481 atattatgtt tttagctttt tcattatctt caatgattac ttcttttcat attagtggta
     6541 cgcaagcggg tttcatttca acgatcacta atttggggat gctcattgga ggtatttttt
     6601 ttggcatcat agctgataaa tatggacgtg tgaaagtatt ttctcaaacg gtccttctgt
     6661 tttcgattgc ttctttattg atgtattttg cttcaaacat ctacttggtt tatctctttc
     6721 gctttatcgc aggtgtagga gcgggaggag aatacggggc gtgtatgtcc ttgatttccg
     6781 aaagtttttc aaaaaaacaa attggacgag cttcttcggt tgctggtatt ggtgcgcagg
     6841 tcggtgcggc tttggctgcg attttagctg ctgtgatcat tccaacgttt ggttggaaga
     6901 tgttgtatgt cgtaggtgta ctgccggtgt tgatggtctt gttcattcgt cgcggcttga
     6961 ctgaaccaaa ggaattcaaa tctgtacaat ctgccgaaaa gaaaaccaaa cttagcagac
     7021 tgtttgaatc aaaacatctt gcttggcaaa catgtggcct tagtatcatg gtaactgtcc
     7081 aaattgccgg ctattttggg ttaatgaatt ggctgccttc aattatgcaa aatcgattag
     7141 ggctttcggt ttctgggtct tcattgtgga tggtcagtac tattttaggc atgtctttgg
     7201 ggatgatgac ttttggcata atcatggata aattggggcc taaactagcc ttttctttgt
     7261 ttttaatttg ttcatcctta tctgtctatc tacttgtgtt agcccatagc cagtggagtt
     7321 tggtagcagc cgctgtgata gtcggctact ttatcaatgg catgtatggt ggatatggcg
     7381 cagtgattag tagtttgtat ccaacagaaa tccgcgcgac ggcgaataat ttcattatga
     7441 accttggacg agctgttggt ggtttttctt ctatcgtcat cggtttcttg atggatcatt
     7501 atagtttaac tgccgtaatc ttgtttttga gcggtattta cctagtcagt ctgctggtct
     7561 tgacaactct tactggtgtt acagcattaa gggaaagctt gcagcattga tagttgagtc
     7621 tgtaaatgac aagaaagagg tgtggcaaaa gcttgcatct gcggtaataa gaccagccaa
     7681 atcaaaaaca tgagacactg tttccgtttg gctggtcgta ttacttgatg cagggcagct
     7741 ttttcaactg ctaatttcct tctatctggc ggcgactatt ctttgacgtc cgtcatttca
     7801 aagtacttta ctaataactt agcataacga aaaactgtgg atacactcgt atattgagcg
     7861 tctgcataat cttccaaatt tttctcagga tgttgataca tgaattcaat caaatgataa
     7921 ttatatgatt ttctgtagaa atgaagataa ataatgtaga ttgggaagtt ctcctgcttt
     7981 tccatagaaa agcgccgatt attctcattc ttcaaaatga ccttatcagg atatttttgg
     8041 aacaattctt ctaaatctgt tagataattc aagatagact tacttgatac cttcaattcg
     8101 ttttgtagtt cttctattga acaccaacgt tctgtttcaa gcagtaaatg aatcatttta
     8161 attttctttt gagaaacttt tgttaaaaaa gtctgcatat tttctcctcc tgtatctact
     8221 ttactatact acatacaaga aaaaaaacgg atacaaatat catattgtca caccgtcaat
     8281 agtgcataaa cctgttatta tttctctttt tttcctaagt attctattga tatgaatcat
     8341 ttaatcgttt attatataaa tgtcaataat ctaataataa aataacgtac atattttatt
     8401 attgcatact taataacaga actatagcaa aaaatcccgt gtatcaacac gggattagat
     8461 taagtttcct caatcactat tcataacaca attattataa caagtaagta aacaattgaa
     8521 aaggacattt attgaaaacg gttgtttttt acttaaggag gaaatttagc gaaaaaatta
     8581 gttgcattgt ttttggtcgc aggaagtttg ttgagtgggt gtggtgctac tacgtctact
     8641 actcaaagca caagttcgga tcaggcttcc agcagcagta ccacgatctc cgaaagtacg
     8701 gcagattcat cgaaagaaca agaacaaatt gaaaagaaaa aagaagagaa acaaaaagcg
     8761 gaaaaagctg cactagaaaa aaagtgacag aagcggaatc tgctttgtca caagctgaag
     8821 caaatcctac aaaagaaaac tatgcaactg cttcagcagc tatccaagcg ctacctgaaa
     8881 acaaacaaga gttgacaagc cgtcttgctg caattgattc cacaatcaaa gcaaaagaag
     8941 cagaagaaga acggaaaaag caagaagaaa ttgcacgaca acaagcagaa caagcgcaac
     9001 aagaagcaat cgcacagcaa caagcagaac aggcgaggca agaagcaatc gcacagcaac
     9061 aagcagaaca ggcgcgacag caatcagcag ctgaacaggc ctctcagcag gcacaagccg
     9121 cagctcctga gcaaaataat gaacaaactg tctatgtcac accgaccggt tcaaaatacc
     9181 atacacataa atgtggaaat ggtacgtata cccctgccac tttaagcgaa gctcaagctc
     9241 gtggtttaac cccttgctcc aaatgttatg gaaactaatt caaaagttat actataataa
     9301 taaaattctt ctgaaacata aaaaaataga agatggcgtt ccagatagta aaccaaaaat
     9361 aacagtttac acgtccattt cgtcagcttc tctctttttt catttagtct gttccctctg
     9421 cttttttgga catcttcatt cattctttct caatcaaata gtacacctct ttttcttttc
     9481 gcagtttata tcttcctttt ttcaatttca ttgaaaactg tttcgaatgt ttatgtttta
     9541 catggatttt aagcaggagc tcctcatgtt gcaagcgata gttcctgcct tttaaatcag
     9601 aaaatctcac tagtccgtat tcatcagaag ctttcacaaa tggaactttt tctctagtaa
     9661 tatagtgttt gccgttgagc gtttttacag aaaagaccat tccttcgatt ggtcgtccgt
     9721 ttgtgttctt tacataaaaa gataaaggat agtttcgctt agcgatggcc aaagaatcta
     9781 aataagcgat gagtagccaa actaagccga ttgatccaag tagtataccg ataaaccaaa
     9841 taaaaaattt ccattttttc cattgatcaa ttccttggat cgcttttttt gcctcttttg
     9901 cttgataagg aatacgatgc cccgtcacaa gcagtcgatg tgtattgacc atataaggag
     9961 tacaggtaag caaagtcacg agatctctgc ctttttggat acccaaagaa tctacttcat
    10021 caggtaagac cacttggatt ttttctactt gatacgccaa tgtctttcca ttgatctgga
    10081 tgaaaaactg gtctcctttt ttcaacttag gcaaatctgt aaataatttt gcttctggga
    10141 gtccacgatg cccagaaaga acggcatggg tgtttttccc gccagtcgga taagaacttt
    10201 cttccaataa tgacgttcct ttttgcaaaa atatttccgt tgtctgatcg aatataggta
    10261 agctgacatt gatttttggg atttcgatga ctccaagcat atgttccata aaaaaggcat
    10321 tactaggtaa atcttttaat gcttttgcat caactgcttg gttgaaactg cctatccctg
    10381 gtgatacgtt ttgctctgcc aactgttggt ttttctttgt cattttttcc tgctgttttt
    10441 ttatttcctc tgaattctta ttcgatgctt gcttttgata atggatgatc atttgctggg
    10501 caagaaaatc attcagtgaa tcttctacaa aaggataaga aaagatcccg atcccaacga
    10561 ctaaaatgat tgccatgaag atgtccagta ttcttattct gcttttattc atcatgtcca
    10621 cctattttct aatatgtata tgaagaggtt gtgaaatcag cgccttgacc tgagtattag
    10681 cgaagcaaac ggcaaaatag ttcctcctat tttgccgttt gttgagcgaa taccaagaga
    10741 ccgaccactt gtcacaacct ttctccccat ttgaatcact atcgtttatt tatcgtatga
    10801 tttttgtttc caatagttta agcttctgtt tcttgatggc gttttttgaa atagatcact
    10861 gctcctccaa ctaaagcagt acctaccaag atgaatacga tgatccccat accaccagtt
    10921 gaaggcaaga ttcctttagg ttggttatcg atcgttgttt catcagaagt tgttggatca
    10981 tcatctactg taaattcagt gtacgcagct tcattcttag gtaatgcgta gccttctggt
    11041 gcttttgttt cttgaaggca gtatgcccct tgtttcagcc cttcgattga aaatttccct
    11101 tctgaatcag aaatcaattt cacttcttcc cagtcagaac ctgtgacttc atctgaccaa
    11161 gcatatgggt attctcctga ggcaaggttt gctatttttt tcattaattt aacagttgga
    11221 tcttttgctc ttagaataga aaattctgca tcggctaatt tttcttgcgt gttcacatct
    11281 tgtttgatga agtggaaatc accgtagttt tggacatttt ctacagtaat cgtgttatcc
    11341 gtagttgtat tttcccgatt gactacaccg tttggattcc caagattggc ttcatctgcg
    11401 ccatggatga taccggttgt ttgttgcaat tttccgtttt ttactataaa agtagtttct
    11461 ttttgtggat tggttaattg ataaccatct ggtgcttttg tttcaactaa agtataattg
    11521 ccgtctatta atccattgac actgatggat ccttcagtat cagttgtgaa tgtcaacgct
    11581 gactcagcag gatcatagag agcgacacca gtgttttcat caaaaccggt cacgaattga
    11641 ttctctgaat ttttgatctg gaattgcgcc cctgctaact tatctacccc tgaaaagttc
    11701 ccagctttct ggattttgat attgctttct ttgatgacat ttttagcata taggtgaatc
    11761 gtgtctaaag gtttgtcagg gtctagggta agcttaacac gatatttggc gacgtttcga
    11821 caccgggctt tggtgtttct acaaagacgt atacagcatc tttcccatca gatttacctg
    11881 gaagagtcag gttagctaat ccttcaccag aagtcgttac acttgcgaag ggtgtagcgt
    11941 ctgctggaac tgctgtgtct ttacttggat agtacgtagc agtgatgtca tatacgttga
    12001 attcaacgcc gttcaacggt tcgctattcg gaaatgtcaa ttccgttccg ttgtttattt
    12061 gattttctgg tgctgaagtg aatttcaatt tatgcaaatt aaccgtaaca ttttctggtg
    12121 taggtgatac ggctgcagaa acctctgcag ttcctaggcc gagaacgaaa ggaagcagca
    12181 tgatagcagt tcctaaaaac gtccatattt ttgttttgat gtttttcatt ttatttccct
    12241 cctattgttt cctttttgtt gttttttata aaaccaagct gtccctgttg aaaggactag
    12301 caataaccct aaaattgaga aacctccaag catctcttcg cttgtttttg gaaagcttcc
    12361 tccatttttt tcatttgatg gaccatttgt ctgcatctga actggcgttt ttccattatt
    12421 cgtcggattt gcttttggtt cgttgatgat ttccaatggt ataggatgaa gcggatcata
    12481 actgcctttt tcaacttcaa agagaatggt tgattgactc aatatatatc ctttaggcgg
    12541 tttaatctcc actagttcgt aacttccaaa agacaatcca tggacagaaa atgttccatc
    12601 tttactggaa gtaaaaatcg ttgctcttga atcatctttg gctacccaat gatttatccc
    12661 gttttcttgc gctaagtatt ggtcattttt tttgactaga aattgagcat ctgctagttt
    12721 ttgtttcgtt tgccgatcca ctttgacaaa ttgtttgcct cctgtttgga tggcaagatg
    12781 tgtttctact gtttgattgt cagtcgtcaa acgtacatcg ttatcaaatg tcgttttttc
    12841 actgttttct ttcaatgtca tttggtaacg gatcgttaat ttttttccag caaacgaact
    12901 aagatctttt ccagaatctg ataagacaaa accatgtttt attttttgtg tgtgaaatgt
    12961 atgaatcttg tctccaccaa tcaaaaaatc gatcgtttct ggttctagcc ataactgagg
    13021 atcagctgta tctttgatcg tataggttcc tatctcgttg atgttctcag gaattgttac
    13081 ggtgatgaga taagggactt tatccccatc tgcaaaatct gtctgcttat tctctactgt
    13141 ttttgttaat gtgagtgcag caggtatttt ttgttcgctc tttggataca agtggatggt
    13201 cgtcaattta tgattagatg aatctgtcac aggaagcaca acgacaaatg gtgctgattt
    13261 ttcttttact taatctgaag tctttatttc cgtaaatcgg taaacagcgt cacgaccttg
    13321 ctcatctttg tccgatgcac taaaagaagc aatgccctct tcctcgtcta ctgtttttgt
    13381 cacattttct gccagtattt tttcactatc acttttttgt gctagttttc tttgtgcgtc
    13441 ttccacacta cttccttctg accgcagctt gtaaaattca tccgagacat catatatagc
    13501 gaaagtaacg ccgttcaacc cttttactcc ttgaaagtta tccgggatat ttattccgtc
    13561 gttttgactg atacttggca tcttatcgct ttcgaacatt cgattatgca aagtaaatgt
    13621 aataggtgat tctctagctg acacagtcgg agaacctata aaaaagacca gtaggataaa
    13681 aaagagaaca gcaaatatat ccacacgtac attttttgtt tttttcatcg ctgttcacct
    13741 gcttttttct tacgatggat aaaataaaag aagccagaaa aaataaagat cagtcctaaa
    13801 aagacaaagg gcagtagacc tatcccacct gtcgaaggaa gcaagagagt cggattgatc
    13861 gtcaacttag cggtatctgt tgcgataatc gtataattat tgtttacact tccttcgcct
    13921 aaactaatcg ttactatttg tgatccaata tctgtccggc tgttaactgt tagggtataa
    13981 tccaatgatt cgcctaagac aacaccggta acgtggagat ttccaaccga aatcgagtga
    14041 ctttgggcat cataattaaa ggcagcgtca tcgacagtaa tcactgcgtt agctttatag
    14101 atggtataag ttcctgatgt gttggctata tcagtaaatg acaacttggg atttgctgct
    14161 ttgactttgt ctattccgct attcgttagt ttaattgtat agctgccagc attaaccata
    14221 ttttttgctg attgtgcatc tgctgtgtaa tccatactgc taagtgccac ttgtactgga
    14281 gtaagcgaac tagtgacacc ctctgctagc ttgacatgga ttgacgggac acctgctttt
    14341 ccatcatatg tcttactgcc actgagctct accgcaccgc tgatttgctt aatcgtatag
    14401 acatcagatg tctgcacgat agtaaataaa tagtttgttc tggcagctag tttagctagt
    14461 ccgctttttg tcaattgcca agacgtatag gttccaattt cagtaggcgc agttacttca
    14521 actccctttt cattcatcgc tttgaaatca gttggattcc atgtgatttg atcaatactg
    14581 agatcattgt ttaaaaggga tgttgattct gttttatcat taaaggcata tttaacgatc
    14641 ggtacaacag aaagaccatc atagtactta ctgccatttg cattgattga tactgttgca
    14701 tttgtcttat aatataggat gacttcagaa atctgttttc catttgcgtc ctttacgatt
    14761 acatttccat ttgtatctaa actagctaaa acactcattg ctgattggtt ctttgtttct
    14821 atcgctgaaa cagctgtaaa ggtatatccg ttgatagtag gcgtattagt cgtcaggtct
    14881 tgtgcagcac ttccgtaagt aagattcatt attttagaag gagcaatcat ctgtccaaaa
    14941 tagtcttgat acttaatagt gattggtgtt tgtttgcctg tataggtcac cgtatcgacg
    15001 ttactttcta gggctgtgaa agaaccgttt gtttgtgcaa aagaatagcc cgttactgct
    15061 gttggagtga taaccacttt gtcacccaca tggattccag ataatgtagt cccgtacgtt
    15121 ccgatttttg ctgctccacc attgactgta taagaaaaag ttcctgtccg attcgcattg
    15181 acctgaaccg ttgctgcttg ataggttgga aaatttgaaa catttccctt accatcaacg
    15241 atctgcccat ttttgatcga gtaccccgtg aatggaatat catttgctaa ctgtataccg
    15301 attgaaaaag tactcatacc tcctccggca ggaagccctc cccatgttga gggtgtaaaa
    15361 gaataacttt tatccgcatt gggtgtaact gaatcgatcg tatttccagc ggtagccacc
    15421 cccatataga tactttttgg gaatgtgata gtagtaccat ttactgtgga gccaataaac
    15481 agattatgga tatctggacg gttatcaata aaagcgattg ctttggttac gccttgtaac
    15541 gcagttttat ccgcactcat cgatccgtta accgcaacaa tgactccatc tttggcatat
    15601 atcactgtat atccatctgg tatggtatgt ccgttgaaat tcgcagtggc cgcaacagta
    15661 ctaaatgtct ggcttctacc tgtttctgaa taagctttct cccctgctgc ttttgctgct
    15721 gtgatttctt tttctgtcgg atcaatcagt tgagtcgagg atgtggttga ggttgtctcg
    15781 ttgttgctag actgattggt gctttctatg gactgtattt cagaataggg tggactattt
    15841 gtctcctctg tcgtttggtt agcaaaattt gacgtttctg tataaacgtt tacataaata
    15901 cttgataagc tccctaagaa agtaaaaatc aagatgatcg aaatcataat gggatgtaag
    15961 tattttcttt tagatacagc tttgagtcta gaaacaaaat gtttttgaca tatacgttgg
    16021 attttatttt gagttttctt aatcattttc atccctcctc tcaataaaaa atcatcaaat
    16081 aatcctttac acgttattct aaatttaatt tttattattt tattgatgta tagaaagttt
    16141 ggggcttttt ttctcttttt attctaaggt tattttagtc ttgtccttta atacaaagat
    16201 tttattttta aataaaaaat actctttttt tacgattatt gaaaaaaaaa gataaaactt
    16261 gattaattga agtattgctt aaatttggaa gctatgaggg aagaataaaa gagttaggac
    16321 gggattatct actatttttc tttgttttaa atatttgtta tctttgaaca taaaagagaa
    16381 atgtgtcgac tgcagtgttg tgtccctcat cacgaataaa cggtgttcaa ctagctgatc
    16441 ttgtattcac ttaaaaaatg gtaaagtacg agttgctatt ccccattttc aattttctaa
    16501 cgctcaggac aaaatactgt tttcacaacc tcattggaat aaaagcatat atattgtttc
    16561 aagaacgaaa tcttgttatc tttttaaagg gcatctgtcc aaaatctaat tttaggaggt
    16621 acgttttggt atttattcgt acaaataaaa gagaattatc tttattcatg taacgcatcc
    16681 aattgatttt ttcatgtacg ctcctggaac gattttactg acgattgctt atttaactgc
    16741 gaatcgctca ttagctttta tcatagctat ccatattttg aataacgttc ttggctttgt
    16801 attatagata cagatgacaa aaaaagttca agtatgcggt gtagaaccaa tatcgcatac
    16861 ttgaactttt ttcaatgagt ataaattaaa acttctaatt ttcaattccc atcatctgat
    16921 atgcccgacc gtctgaactt cttttgaaaa agccaaatgt gattagataa cgtctttgtt
    16981 cgacataatc gccactagaa aatacgttca gcagtatcga attaacctct gtttctgtat
    17041 aagtcactcc atggtcaaag cgtttcaaga cttctttcaa taggatgtat ttcttttttg
    17101 ttgaacttgg tacttgtttt acttttccat cattcatgaa tgtgtctaaa actttcatct
    17161 tttcttgctc cgtataagta taaaccatct tctttctcct tttgattcta agaatactcc
    17221 atttatgtta gaatattctg gattttttat taaagcggtt actcttaaga aagatattat
    17281 catagtgctc ttttttttac tactacttat aggtagattt attctaatcc attattctcc
    17341 cctaccaata attaaagaaa aaactggatc agggtgttca aattacatgc taagaaaaaa
    17401 gaacccttga ataccaaggg ttctttctca gtctgataac aatgattaac gacggatttc
    17461 tttgatactt gtgaaaagtc gttaaagtat tgatataaaa ggatttttat tacctctgac
    17521 tacatattga ctaatttgtt aaaaaatcgt ttttcaagaa tcctgtaata ccagtgcttt
    17581 caccgttttt ttaactctct aaagaactaa aagcaatcag aggttttacc ttttcatttg
    17641 acgttaagtg gcgataaatt ttcgaagttg tttggtcagt atggcctaag cgatctttca
    17701 agacttccca gctacagtgt tccgctacta agcttgccat tgtatgacga gctaaatgag
    17761 tatgaaatgg cttattaatt cctgagcgat tcacgcaccg tttgacgtga tcatttacat
    17821 actctcgacg ataaggaaca ccacgtttac tagaatcaag atacacaaac aaatatgttt
    17881 cttctataat aaacctgtaa tgttgcctca tctcacccaa agccttaatc aactgttgta
    17941 gcttttccat aactagagga gttactagaa ctcgtctagc agaactaact gtcttagtag
    18001 tttcaattcg aaaatcctta ctcttacctt taactgccgt ttgcttatag aacagaattt
    18061 catttctttt gaaatctata tctccaggct gtagagcaag cgcctcgctg actcggcagc
    18121 cagtgtaaaa tagaacatgg aaaagtacag acatcggtaa ttcctcatga acaatgccat
    18181 aattcaaaaa agttctcaat tcatcgacag taagaaactt gtcctcaata ttatttatgg
    18241 cttcttttaa atcagttacc gttaactgat attttggcat tctcacagac ttcattggat
    18301 tttcacttat caaattcttt ctttgacaat atttaaaaat catatttaga acttgaataa
    18361 gtgattgtaa actatttttg gcatagccaa catttgttcc attaacgcca cattccttta
    18421 aggtaaaaag caattcttga aggcactctg gtgtcaaatc agacaacttt ttatcaccaa
    18481 tccatcgaac aataattttt aattgttcct ctcgcttatc ataggttatt tccttttgtt
    18541 catttaacga acggtaatat tggaaccatt gctcacctac aacactaaac ttgtcattgc
    18601 ttgcaacaat ttttttctta agcctttgtt gtttctccat ttccagataa gcgagttgcg
    18661 cctcacgttt tgaattaaag ccacgtcttg ttgttcgata tgcttgaaag taccatgtga
    18721 tcctaccagt agaactgatt tgcttcttga atcgatctgt tttttccatt tattgattcc
    18781 tcctattttg aaggaaccca acaagcagaa accctttgga aatctttcta tctatgtcat
    18841 cattgtaccg catcttagaa agaaaatgta acttataaag atcgaagtag tcgcaatttt
    18901 tctcgatagt cattcgaatc actctttctc atccattcta aaacatcatc acgaaaatag
    18961 tgtcctcgaa tcccgtacca acagggagga aactcaccaa gttttactaa acggttcagt
    19021 gtactatcac ttatctgaaa tagtcgcttt aaatcagtat ttgttaatac cagaggtaaa
    19081 tgactgactg caagcaattt ttccgtgtaa tcttccagtt ttttttgtaa aattgaatag
    19141 aactctattt ccagagaatt aacatttgtt ttaacttcca actccatcac ccttctctct
    19201 caaaatcaaa acgaccacca cggtacccct taaaattgta aaaaaacact ccagtactcc
    19261 atcaaaacgg actagaaaag aggaattttt aatatgaaac aaggaattgc agacataaaa
    19321 ataattaaag aaattcttga aaaatctacg gctaatgcaa tagcattcgg aacaggaatc
    19381 aacttgagta cagtaaaaaa acttaaaagt ggagagagag ctgaagaaaa attaaatctt
    19441 gccgatgcta ttaaaatcac agaatttggc atgaaaaata tgccaaccaa aatcgaaata
    19501 tggaaatagt agtttttctg atctatcagc tgttttgcca aagcacatag gaatctcacc
    19561 tccccccagt aatggcgggc cgatttggtt acggaagtat cattatcgta gtcgcgattc
    19621 tcatattaaa atttgtcgct taaaagctaa ccttgtcatg gcgtaactag agaatcaaaa
    19681 tctacatgta ctgatagatt tgctattcat ttgtcaaaga gcgctaggct attttcagcc
    19741 tatggaaatc ctttattttg acccgataaa aaacttccat agaatgaaaa ggaaagggaa
    19801 aaaatacttt aacgagttgt atcaaattca agtatcttct caatcaattt tgtttccagt
    19861 tgtctacgca tatattcatc tacatacata accattcctt tttcttcttc ataaactggt
    19921 ttcatggcta accgtgaaat ataattttga tattttgaca gaacgatgga catggcaatt
    19981 gaatcaccag aagctgcggc tttgattgtc gttagtggta tgaatggtat atatcgttta
    20041 ttttgcttca tatttgacac ccaaatactt tcctagctct tccaaagcca tttgtcgttg
    20101 gtaccaaaca ctgccgagac ttttcccaat aatatcgcca atttcacgat cattaagacc
    20161 gatgaagaag gacataaaaa tgatttcttg tctttgacta ggtaatgctg caatcgcatt
    20221 tgccaactcc tcattggaaa taaagatttt cataccaagg acaataaaag taccactact
    20281 attcaaattt ggttccaaat aatagaactc tgtacctaac cctttttgtt ccaatatttc
    20341 cattgatatt tgccgttggc gaaatctagc gtacttcttt tgaatattta acgcctcatt
    20401 gcagactact ttcttacaaa aactgtcaaa cgtatgttga atgtgggttt cctctgagtc
    20461 gcccataata ttctccccct caaaaaattc attcaactcg ttttacttct atccctttcc
    20521 attattacta caatctagga
//
LOCUS       DACEPW010000033        20469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEPW010000033 DACEPW010000000
VERSION     DACEPW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.034"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(133..306)
                     /locus_tag="IW819_000964"
     CDS             complement(133..306)
                     /locus_tag="IW819_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1244084.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IW819_000965"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IW819_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IW819_000966"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IW819_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1244085.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IW819_000967"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IW819_000967"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1244086.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IW819_000968"
     CDS             5704..6489
                     /locus_tag="IW819_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1244087.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IW819_000969"
     CDS             6502..7353
                     /locus_tag="IW819_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1244088.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IW819_000970"
     CDS             complement(7477..7929)
                     /locus_tag="IW819_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1244089.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IW819_000971"
     CDS             complement(8394..8762)
                     /locus_tag="IW819_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1244090.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="IW819_000972"
     CDS             8912..9394
                     /locus_tag="IW819_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1244091.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="IW819_000973"
     CDS             9431..10441
                     /locus_tag="IW819_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1244092.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="IW819_000974"
     CDS             complement(10556..11269)
                     /locus_tag="IW819_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244093.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="IW819_000975"
     CDS             complement(11459..12049)
                     /locus_tag="IW819_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1244094.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="IW819_000976"
     CDS             12533..12760
                     /locus_tag="IW819_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1244095.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="IW819_000977"
     CDS             12776..13942
                     /locus_tag="IW819_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1244096.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="IW819_000978"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="IW819_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1244097.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="IW819_000979"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="IW819_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1244098.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="IW819_000980"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="IW819_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1244099.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="IW819_000981"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="IW819_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1244100.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="IW819_000982"
     CDS             complement(19155..19820)
                     /locus_tag="IW819_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1244101.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="IW819_000983"
     CDS             19796..19969
                     /locus_tag="IW819_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1244102.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..>20469)
                     /locus_tag="IW819_000984"
     CDS             complement(20014..>20469)
                     /locus_tag="IW819_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244103.1"
                     /translation="LSILENIILAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPE
                     MLSGGQKQRVAIARALAMNPDIMLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVT
                     HEMGFAKEVANRVMFIDDGNFLEDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT         6879 a         3485 c         4293 g         5812 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagaccat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaag
//
LOCUS       DACEPW010000034        20342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEPW010000034 DACEPW010000000
VERSION     DACEPW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.035"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(141..416)
                     /locus_tag="IW819_000985"
     CDS             complement(141..416)
                     /locus_tag="IW819_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1244104.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /locus_tag="IW819_000986"
     CDS             complement(659..1969)
                     /locus_tag="IW819_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1244105.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="IW819_000987"
     CDS             complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="IW819_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1244106.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3515..4195)
                     /locus_tag="IW819_000988"
     CDS             complement(3515..4195)
                     /locus_tag="IW819_000988"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1244107.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4244..4879)
                     /locus_tag="IW819_000989"
     CDS             complement(4244..4879)
                     /locus_tag="IW819_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1244108.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4945..6348)
                     /locus_tag="IW819_000990"
     CDS             complement(4945..6348)
                     /locus_tag="IW819_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1244109.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6335..7366)
                     /locus_tag="IW819_000991"
     CDS             complement(6335..7366)
                     /locus_tag="IW819_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244110.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7397..7618
                     /locus_tag="IW819_000992"
     CDS             7397..7618
                     /locus_tag="IW819_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1244111.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7783..8520)
                     /locus_tag="IW819_000993"
     CDS             complement(7783..8520)
                     /locus_tag="IW819_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1244112.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8673..10013)
                     /locus_tag="IW819_000994"
     CDS             complement(8673..10013)
                     /locus_tag="IW819_000994"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1244113.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10250..11773)
                     /locus_tag="IW819_000995"
     CDS             complement(10250..11773)
                     /locus_tag="IW819_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1244114.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12152..12763)
                     /locus_tag="IW819_000996"
     CDS             complement(12152..12763)
                     /locus_tag="IW819_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1244115.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12886..13011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13093..13809)
                     /locus_tag="IW819_000997"
     CDS             complement(13093..13809)
                     /locus_tag="IW819_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1244116.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW819_000998"
     CDS             complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW819_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1244117.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14368..15165)
                     /locus_tag="IW819_000999"
     CDS             complement(14368..15165)
                     /locus_tag="IW819_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1244118.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15135..15506)
                     /locus_tag="IW819_001000"
     CDS             complement(15135..15506)
                     /locus_tag="IW819_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1244119.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW819_001001"
     CDS             complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW819_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1244120.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(16508..16981)
                     /locus_tag="IW819_001002"
     CDS             complement(16508..16981)
                     /locus_tag="IW819_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1244121.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(17086..17949)
                     /locus_tag="IW819_001003"
     CDS             complement(17086..17949)
                     /locus_tag="IW819_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1244122.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(18145..20244)
                     /locus_tag="IW819_001004"
     CDS             complement(18145..20244)
                     /locus_tag="IW819_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1244123.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT         5528 a         4349 c         3434 g         7031 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     2101 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     2161 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     2221 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     2281 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     2341 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     2401 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2461 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2521 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2581 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2641 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2701 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2761 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2821 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2881 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2941 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     3001 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     3061 acttttttag catctaaacg gcgttttgaa agtaggtagc tgccgttttc tttatctttg
     3121 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     3181 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     3241 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     3301 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     3361 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3421 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3481 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3541 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3601 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3661 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3721 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3781 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3841 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3901 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3961 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     4021 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     4081 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     4141 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     4201 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     4261 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     4321 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     4381 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4441 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4501 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4561 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4621 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4681 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4741 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4801 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4861 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4921 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4981 aatcgtttcc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     5041 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     5101 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     5161 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     5221 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctt
     5281 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     5341 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     5401 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5461 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5521 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5581 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5641 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5701 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5761 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5821 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5881 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5941 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     6001 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     6061 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     6121 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     6181 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     6241 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     6301 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     6361 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6421 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6481 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6541 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6601 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6661 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6721 aaaggtggat acatctgctt tgttgcagat aaacctctga ctcatcattg ttttttgcca
     6781 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6841 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6901 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6961 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     7021 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     7081 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     7141 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     7201 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
     7261 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     7321 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     7381 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7441 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7501 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7561 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7621 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7681 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7741 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7801 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7861 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7921 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7981 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     8041 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     8101 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     8161 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     8221 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     8281 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     8341 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     8401 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8461 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8521 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8581 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8641 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8701 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8761 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8821 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8881 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8941 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     9001 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     9061 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     9121 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     9181 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     9241 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     9301 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     9361 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9421 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9481 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9541 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9601 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9661 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9721 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9781 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9841 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9901 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9961 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    10021 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    10081 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    10141 gaaaaaacac tcactgaatg gagctattga cttgtccacg tgcttatcga tagccgttta
    10201 tcggtttctt cattcttatg atagaccttg tacctgtatt tgtctaggtt tagaacattg
    10261 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    10321 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    10381 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10441 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10501 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10561 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10621 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10681 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10741 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10801 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10861 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10921 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10981 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    11041 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    11101 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    11161 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    11221 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    11281 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    11341 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    11401 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11461 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11521 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11581 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11641 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11701 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11761 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11821 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11881 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11941 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    12001 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    12061 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    12121 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    12181 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    12241 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    12301 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    12361 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    12421 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    12481 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    12541 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    12601 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    12661 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    12721 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    12781 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    12841 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    12901 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    12961 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    13021 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    13081 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    13141 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    13201 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    13261 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    13321 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    13381 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    13441 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    13501 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    13561 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    13621 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    13681 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    13741 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    13801 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    13861 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    13921 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    13981 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    14041 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    14101 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    14161 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    14221 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    14281 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    14341 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    14401 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    14461 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    14521 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    14581 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    14641 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    14701 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    14761 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    14821 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    14881 gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
    14941 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    15001 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    15061 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    15121 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    15181 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    15241 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    15301 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    15361 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    15421 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    15481 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    15541 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    15601 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    15661 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    15721 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    15781 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    15841 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    15901 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    15961 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    16021 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    16081 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    16141 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    16201 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    16261 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    16321 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    16381 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    16441 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    16501 tagacactca gacggactgg tttctttttt ctttttcata gcattcctga cagattccat
    16561 ggaacgttag ccgatgatct tttactaaaa aatgataacg ttcttctact acttgctcca
    16621 cttcaagtaa aagatcctct tctacttcct cgatcgtgcc gcagttcaag catagtaaat
    16681 gatgatggaa atgtttggcg ccttcttttc tcaaatcata acgggctaat ccatcattaa
    16741 aacttacttt atcgacaatt ttcaattctg tcaagatttc aagggttcga taaacagtag
    16801 caagtccaat ttccggactc ttttttttca ctaaaaagaa gatttcttct gcagataaat
    16861 gctctttctc attttccaaa agcacaagca gagtagcttc tctttgcggt gtcagcttaa
    16921 accccgcttc atgtagctgc tgttttgttt ttttcatcgc cgaaatagca ttcatgacca
    16981 tttactcaac cccaacaatt tataataatt acaaataaaa aaagatataa cactatgtta
    17041 attaaaatta ttataagcta gttaagaaaa acatgcaagg gattctcaat tagatttttt
    17101 gactagtttt gtcacaccgt tttcttgtgt aatcaatcct tgtttcaaaa gattacctaa
    17161 tgcgcgctta aatgctcctt tgctgatacc aaaagctttc atgatttctt ctggatccga
    17221 tttatctgta aagttgattt gctgatctgc agaacgttct aaaatggtta aaatcatttg
    17281 tgcatcatct gatatagctt catatgctct tggcttcaat gaaagattca gtacaccgtc
    17341 tggtcggaca ccaatcactc gaccttttat ctgctcgcct agacgaggtt cattatatcg
    17401 ttcagaagga tggataaacc caagatagaa atcttccgtt aaaacgtacg tacctgttaa
    17461 tttcaagcgg tatgctgttc ctatcacatc tttgtttttc atttcttctg ttccatattt
    17521 gcttaaagat ttgaatattt tttcatctgc taaactgccc caaatacggt cttttgcatc
    17581 aactctaagg gaaatcatca agcgatcccc ttttttaggc catagctctg tcattgtcgg
    17641 caattcatca agagatacga caaagtcttt atctggaaga ccgatatcga caaacgcacc
    17701 taaatctttt cgtgtagcag taacgattcc aaaagcgtaa tgaccgatac ggcttttagg
    17761 aatttgtgta gtgatccgat tttcatgttt ttgattttgg taaccgaatc cttcaactgc
    17821 ttcaccgatt ttgtgttcac cttctgattt gcttaagcca aaggtttgtc cgtttttttg
    17881 gacaaagtag tttttttcat tttcatcaat aataatacct gtaaatatag ttgctaataa
    17941 atcgttcatt tttttctccc tctatagtga aaggttttct taatacattt ctttgctatc
    18001 ataacataat gttatctact tgaatagctg agccttggtt tggaaaggtg aaaaaaggag
    18061 tatgacaaca gttgtgtcat actccccttt ttcgaataaa cggtgttaaa cggcaggctc
    18121 acagcctctt tcatttgcct aaaattaatt gctcccattt cctccgaacc atccagtttt
    18181 acagttgatg gttacgatgt aaaatggcaa agcactttct ccaatgcctt tcatcaatgc
    18241 agattttgtt gaagtggcat atagattata atagccttcc ccattttcga ttctggcttt
    18301 ttctaatctg taacctcctt caacgatata tccacgttgg atacatgtag caatcacttt
    18361 gctttggaca cctggtgccc acgtccatgc tggattagat gcatcggcaa tatcacttgc
    18421 tctggcagat ttgattggct gattcgctga gtttgaagag gaaggggtcg aggatgccgc
    18481 ttcttgctgt gctgcttgtg cgaccgcagc ttgttgagcg gcttgagctg cagctgctgc
    18541 ttgagcggct tgttttgctt gttcttcagc tgcttttttc gcttcttctg cttttttctt
    18601 ttcctcttct tcttttttct ctttatcagt gagcatctta tctacttgtt tcaattgttc
    18661 attcaactga gcagatagat ctgtattttt cactttttct actgcttctt ttgccgtctt
    18721 gtattggtcg cgtgttgctg aatccactac ttttccgtct ttaaagacta tagctgtttt
    18781 ttctttagct gtttgcaatt cagcatattg atttttagct tctgtttgtg cttgttgaat
    18841 caattttcca aaatctgtgt tatccgtttc gattgcttgg atctctttat ctgctttcaa
    18901 cttgatgtca tctgctagat gatcatcgac gatggcaggt gctatgaacc actcattcac
    18961 tttttggata tctcggatct tagattggat atcttggtaa gttgtttcta actctgaata
    19021 tcctttttca ttcttaagac tggataaatc attttttagt tttgataaat cttgatgaat
    19081 catatctgca cgaataaatt gatggtgatc atctgtataa aaggcagcaa gtttctcatg
    19141 aagttcttcc atttgttgct gtctttttgc ttctgctgct acttcggctt ctactttttg
    19201 atgatggtca taataagccc agcttcctcc accgagtaga agtactgctg ctaaaacgag
    19261 ataaggtact ttttttgatt ttttcttttg cgctttatct ggatctgttt tttctaagtt
    19321 tgattcttga tgtgtttttt cagtatctgg cacgattgtt attgatttcg cttctgcttc
    19381 aaacagctta ggctggttct cttctttttc tatttttgct tcaagatcag taccctcttt
    19441 gcttacagat tgattttcag catcggattc actatttatt tcatcgggct ctgttgactt
    19501 tttgtctgtg ttttcgagta tttctttttt ctctgcttcc ggcagtttat tcgttttttc
    19561 cttttctaat tgtttttcag taaatacttc agttttttct gtgatatccg tttttacgtc
    19621 ttttattttt actgtttctt tacctgtggc gttttctcta tcagagtctt tttcagtttc
    19681 ttgttccttt tctggctcta ctttgttttc ctgtgcaatt ttttcactga tcattttgag
    19741 cagctctttc gcgtgctctt cttttgtatc ttcacgatgt gctttgatat aagcggatag
    19801 tatcgaattt tcttcctcat tatcgacgat ttctttcagt tcagctactc ttcttttttg
    19861 ttttgattca agcgtttctt cttgattctt tcctgattct tccgaagtct catgaacagt
    19921 tgctttcttc tgttcatttt tctctatatt agttacttga tcgtgagcag gtggttcttc
    19981 gtcttctggt tctgcttttt cgaataattc cattacagat tctagaggta aatctttcaa
    20041 ttccgaccat tcgattggat cgtttaattc tgtatcagga aagactgcat cttcagttga
    20101 tttatcctgc atagctgcat ctgtatttgt ttcttcaatt gattctttta tttgctgatc
    20161 ttctgcagtt atttgatgac cgcagtctgg acagatagta ttctgctcca tttttttcga
    20221 tccgcaattt ggacattttt tcatcactta gcgatcggct cctttactag gtttacgcta
    20281 ccatttaatc tatcatattc tatacgtcac gccaatagct tatttttagg atttacttaa
    20341 aa
//
LOCUS       DACEPW010000035        20310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEPW010000035 DACEPW010000000
VERSION     DACEPW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.036"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(472..1359)
                     /locus_tag="IW819_001005"
     CDS             complement(472..1359)
                     /locus_tag="IW819_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1244124.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            1544..2383
                     /locus_tag="IW819_001006"
     CDS             1544..2383
                     /locus_tag="IW819_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1244125.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2579..3553
                     /gene="sgrA"
                     /locus_tag="IW819_001007"
     CDS             2579..3553
                     /gene="sgrA"
                     /locus_tag="IW819_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1244126.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            3851..4207
                     /locus_tag="IW819_001008"
     CDS             3851..4207
                     /locus_tag="IW819_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244127.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            4210..5367
                     /locus_tag="IW819_001009"
     CDS             4210..5367
                     /locus_tag="IW819_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244128.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5393..5833)
                     /locus_tag="IW819_001010"
     CDS             complement(5393..5833)
                     /locus_tag="IW819_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1244129.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5993..6499
                     /locus_tag="IW819_001011"
     CDS             5993..6499
                     /locus_tag="IW819_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1244130.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6667..8580
                     /locus_tag="IW819_001012"
     CDS             6667..8580
                     /locus_tag="IW819_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1244131.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            8585..9586
                     /locus_tag="IW819_001013"
     CDS             8585..9586
                     /locus_tag="IW819_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1244132.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(9788..9994)
                     /locus_tag="IW819_001014"
     CDS             complement(9788..9994)
                     /locus_tag="IW819_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1244133.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(10640..11488)
                     /locus_tag="IW819_001015"
     CDS             complement(10640..11488)
                     /locus_tag="IW819_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1244134.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            11652..12554
                     /gene="gnd"
                     /locus_tag="IW819_001016"
     CDS             11652..12554
                     /gene="gnd"
                     /locus_tag="IW819_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1244135.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            12556..13557
                     /locus_tag="IW819_001017"
     CDS             12556..13557
                     /locus_tag="IW819_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1244136.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            13554..13838
                     /locus_tag="IW819_001018"
     CDS             13554..13838
                     /locus_tag="IW819_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1244137.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            13871..14668
                     /locus_tag="IW819_001019"
     CDS             13871..14668
                     /locus_tag="IW819_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1244138.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            14682..15515
                     /locus_tag="IW819_001020"
     CDS             14682..15515
                     /locus_tag="IW819_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1244139.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            15533..16390
                     /locus_tag="IW819_001021"
     CDS             15533..16390
                     /locus_tag="IW819_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1244140.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            16412..16921
                     /locus_tag="IW819_001022"
     CDS             16412..16921
                     /locus_tag="IW819_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1244141.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            16958..17359
                     /locus_tag="IW819_001023"
     CDS             16958..17359
                     /locus_tag="IW819_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244142.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            17471..17824
                     /locus_tag="IW819_001024"
     CDS             17471..17824
                     /locus_tag="IW819_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244143.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            17858..18301
                     /locus_tag="IW819_001025"
     CDS             17858..18301
                     /locus_tag="IW819_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1244144.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            18318..19403
                     /gene="uxuA"
                     /locus_tag="IW819_001026"
     CDS             18318..19403
                     /gene="uxuA"
                     /locus_tag="IW819_001026"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1244145.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            19396..20109
                     /locus_tag="IW819_001027"
     CDS             19396..20109
                     /locus_tag="IW819_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1244146.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         6946 a         3127 c         4241 g         5996 t
ORIGIN      
        1 aaacgatgta ttccctaacc tctaatacat ccttgtaact ctgaagttac gccagttttg
       61 aataaatttc ataaaaacaa atgaaaagtg tcgcataaaa atatctttcg tgtttctcta
      121 gtataacaga ataagaggaa caagtccaga agggatattt ttagtctttt ttaaaataac
      181 ttttcagtcg agtttgagtt tatgatagtt gtttttgaag cgatacggca agtgaaaaaa
      241 tagggaagca tgaaagaatc tacttaatgg cgcattgatt catgtgatag aaataaaaaa
      301 cttttttaaa ttatatcctc atgattgtaa caaatccagt gatttgtttt ggtacaaata
      361 aaaaaaggcc ccgatgaagt tgtggcaaaa gtggtttgcc tcaaaaataa gaaggaattt
      421 ccaaaaatca cttctcaaat tttgccacat ccccaattgg gtcaaatttg tttatttgaa
      481 tatatttttt acataaggat ctttaatagt tacattgtct tttttcatca ctttacggat
      541 tgttttatcc atgaattccg tatcaagagt ttttgcttcg gtagcaattt tttctagctc
      601 agatttgtat ttgtctttat tagaaccttt atcctgtttt tttaccattt ttactagata
      661 atagctttgc tgataagtcg ttggatctgt tacaggaatg atatcagaaa cttccccgtt
      721 ttttagttta aatgctgctt tttttacttc agtaggaatc tctggattgg ttgaatcaaa
      781 attcattttt ccatcagttt cctttaatgt tttatttttt ccataagctt gaacaagctt
      841 ggagaaattt tccccttcat ttgcttcttt ttttgcttta tctgcttctt ctttagtcga
      901 aaatagaatg atctgtgtac taacttctgg atagaattct ttccaagcag tgtctagatc
      961 tttatctgtt aattttacat tggctttcaa accgtattgg aaagccagca tctgtttaaa
     1021 gagtttctta tatgtctctt ctgtttggcc aactgctttt aattgttcct tgtagtttgg
     1081 accgagtttt ttgatttgtt cattatattc ttttgttact tcctttgaag aaatctcttt
     1141 tccgtataca tcatcaaatg ctttgaagac aatcattttt tttaatagtg tttgattatt
     1201 tgatttaact tttgggttat cataaaaatc ctgaactgta agtgttccac ctttaaacgt
     1261 tgcaatcgtt tcattggact tgctacaacc tgagaatagt aaaattgcac atgctgaaag
     1321 gcacataatt agtagttgtt ttttcaaaaa atttcccatc ctttccttat taaaaacagt
     1381 ctaacagaag aattagggag acagtctttc attttcgtaa ggttgctgta gatattctta
     1441 gttcgtaaaa atctattctc tctatgatgg agaacattta catagttttc acgagacaag
     1501 aaagaaaagg gttgttaaac ttaatataaa ggagggatga aagatgatca ggattattcg
     1561 gttgattgga tctgttttaa tagtcttagt tggttttgtt ttaggaattc ttgtcaataa
     1621 tgcacttgcg gatatgttta ttggaaatag tgaatggatt ggagcaattg ccggtacggt
     1681 cggacattta gctgtttttt tactagcttt atttcttgct agtaaggtcg aaggaaaaag
     1741 aatagaagat tatggtgttt ctgttcgagg aaaagactgg gggtatttag cgggaggtct
     1801 cgttgttgga attggagtgt ttctgcttat tactagtcca ttatatttga cgggggcgta
     1861 tcggttagat tcaggaaacg caaacattgt gccgctgata acgagtttta ttttatttat
     1921 tgcagtgggg gcatcagaag agcttttatt ccgtggcttt tttcagcatc agatgcttag
     1981 atttggtccg ttaatagcaa tgattggatc tgctgcatta tttgctcttt tgcacggatt
     2041 gaatcctaac atgacatttc tggcagtttt caatatcttt ttagcaggtt gctttttcag
     2101 cgcacttatc tacagtacag gaagtttatt tacggctatt ggtgctcata ttacttggaa
     2161 ttggacacaa ggagctattt tgggtatacc agtcagcgga acgcaagaac aaggtttttt
     2221 ctccactctg atccaatcgc aaaatacaat aattactggg ggaaactttg gggctgaagc
     2281 atctatatca actacattat tcttgttagt cttgattgtt ggtttgttac tctatgcata
     2341 taaaacaaag cggatgaaga aatacactgc tgtttatcaa taaaagatga agaaagcaag
     2401 gctgatatcg gtcttgcttt tttaagtctg tcattgatta aacgaaaaag taaacaaatt
     2461 caagcgaata cacgacatct tttgttagat tttcacaact gactgtcgtg ttctcatcat
     2521 tttcatttta tataattcct ttatcaaata gaaggtaatt atatggagag tgatagaaat
     2581 gaagaaaaca gcgaccgtat tatttgttat tcttgggctg agtataattg cagcaatgat
     2641 tggaatcagt acttctgcaa atgaacgggc aaatgagaat ctttcattta ctgtgaaaac
     2701 tgatcggatt gtttatgaca tgacacaaca agtcattact attccagtca aaccaaacaa
     2761 aagcgtgaat gctagtgatg tacatgctgt tctaacatat ggttgggatg gtaatggaag
     2821 ttcagaaaaa gttattgggg aagtttattt aaaagacgta caatggaccg ctggtattga
     2881 gtatacaata atgatttctg cagaactatc gattgatgag atcaagagta aagataaagt
     2941 tgatttgatt gttttttatg atggacagat gacgatcacg gagaatttga aaccaagcag
     3001 ttggacagtt gttggaccgc caagcagtac ggatagcaca agtagtgaaa gcagtacaga
     3061 aaacacgagc ggtgaaagca gtacagagag tacgagcagt gaaagtagca cagaaaacac
     3121 gagcagcgaa agcagtacgg agagtacgag tagtgaaagt agcacaggaa gcacgagcag
     3181 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcatga gtagtgagag
     3241 tagcacagga agcacgagca gcgaaagcag cacaggaaaa gtaaataatg aacttgattt
     3301 ggtacctatc ataccaacta aggaacaaaa gagaaatgca gggagcaacc taaaaagtgt
     3361 gctaaaacag cctgattttg tttctcgtgg aattgatcat caagaaaaag cgaaagaaac
     3421 agataataga aaaagcgatt taccagaaac tggtagtaga accttgaaca agctaattac
     3481 atggatgggt gtgttactca ttttgattgt aggagcaagc tattttcgtt ctctcttcca
     3541 tcgagtgaaa taaagaataa aagcaacaga tctaaaattg aataaaacga tagatttttg
     3601 agtgaatatt tcattcgaga gtgctgtatt gcctggcgaa gttgtagctt tgccagatct
     3661 atttttgttt actaacatat ttgagaaaca ggaaataaca ggaacacaaa tggaaaataa
     3721 gagcaagaat aattgacaat gcacactgcg catggtagta tctctgaaaa atcagtaacg
     3781 aatctatttg tacgttattc atgtagctgt cggaataagt ctaaataata gataaaaagg
     3841 agtatgtact ttgtttgaag aatttattga tatcaatgaa aggcaagtat atcaattttt
     3901 gaactattgt tatgaaagag acgagaaatt atatgtggtg aaggatatcg ctttagatct
     3961 aaactacacc ctggctaaga tgaattcggt gattcaacaa gcagaatcgt tttgcgaaag
     4021 gtatccagaa tataaattat cttttctttc agaaaacaag atgataaaag tcgagttttc
     4081 ttctcagttt ttactgagta aagtttattc tatactttta gagggaacga ttggttatat
     4141 tttactggat tcactttaca aaggtactta tcaatcctta gaaaatctta gtcaaaaaat
     4201 tatataagtt tgcgtacgat tcaaagaaaa ttaagagaac ttaatactat tttgaaaaac
     4261 tatcatttat cttgcagctt gaaaaaaggt aatccgatag aaggagaaga ataccgtatc
     4321 cgatactttt ttcacttgat gtattggcag tcattcgacg aaaatcattc tgatatatta
     4381 tcaagtataa aaaataaacg tcaactatta gaaaaatttg cgaaacatgc gccatatacg
     4441 cgtagtatag atagaaagaa atggatcagc ctactaagaa tttcgttgca acgaatgaaa
     4501 caaggccact cgattacgta tgtcccagaa gagatgaaca aatttgtgca tccattgata
     4561 acaaaagaag agtttacgca ttacatacta gagccttttt ttgaagcaaa cttctggtcg
     4621 atgaatcaca tcactccaac agagcaagct tacctttatt ttatgtttgg tgtaatgaac
     4681 acctatttgg aagaagattt gttggatact ccagataaag tacaagaaag ttggggctgg
     4741 attgaatcgt ctattgtaga aaattttgaa aaaaacttca agatttgttt tacaacttca
     4801 gaaaaaaatt atctatatgt caatttggca atgatccatt tgtattcaag ggtttttggg
     4861 acaaaaaaga aggtggatgc ttttggaaaa tcagcagaag acagagattt tcagcatata
     4921 tttccagtga tgtatccaat catgcaacag ttttttagtg gggtagctcg cagaattcca
     4981 gagcttaata cttattatga aaagaacaga cggttgattt ttcaatactg tatgttagtc
     5041 agggatattt ttataaaaca cgaacaacct gtcatattct atattcaatc aaagtatggt
     5101 aaaacacagg aattatgggc aaagaaaagg atattatcta tgactgaacg cccaataaaa
     5161 tatagtgcat ccgctgatca gttacctgat attgtcatta gcgattatcc tatcgacaaa
     5221 aaggcatatg atcaaacgaa tacacagata ttttactgga atggacaacc tacaaaaaat
     5281 gattgggcgc gtctgaaagc tgaaatcata aaaatcagga atgagaaaaa tagatccata
     5341 ttggaaaaat tggatttcgt taattaagaa aagcagattg tttatgccaa aattaagaca
     5401 ttgcaaaaag attattttct ttaaaatcga caagatgacc agaaagcaag agttcgttga
     5461 gctcttgctt tcttttagtt ttagaaagga acatcctgat tttatcttcg ctaaatccag
     5521 tcaaaagttt tgcagattgg tcgtagataa tcggtttttt caataaagaa ggggatgtac
     5581 gaatctcaat atatagttca ctcaaactga gttcatctat tctgtgaaca agatttttat
     5641 aactaataga atgagtagag agcatttcgg tcacgccgtt gtttgtcaat tttagtaagt
     5701 ggataaattc atttttggtt agtggttgac gaattaagtt ctgttcgata aacggtatat
     5761 catggacgat aagccaacgt tttgcgcgtc ttgaagacga gcagccagaa cctgtataca
     5821 atttaatcat catacattcc tccaatagct aagtatttat gaaataataa catgaagaaa
     5881 tagtcgaaaa gtgccattta gaggtgtaaa ataagaaaat gttgtcacgt ataatggaac
     5941 tagctagata agaaatacta ttaaatcaag aaaaaagaaa gaaggcacaa gtatgtcaaa
     6001 cacagccgac gcaatagtag tgatcgattt acaaaaaggg gtatgttttg attcttcgcc
     6061 gatacacgaa ttagaaacag tgacagcatt agtgaataaa agaatcaaaa catacgaaac
     6121 gattgggaaa ccagttattt tcgttcaaca cgaagatgct caattagtgc cacaaacaaa
     6181 accatgggag ctattgtcgg aattatctat tcctgagcac agttattttg tgagaaaaac
     6241 ccatgcgaat tcattttata agactaattt gagtgtttta ttaactgaat tggactgtca
     6301 ttcgatcgaa ttatgtgggg cacaagcaga gtattgtata gatacgactg tgaagtttgc
     6361 tcatggatta ggataccagc ttatgatgtg tagaggagca acgacaactt atgataatcc
     6421 ttttatgaaa gcaaaggaaa caatcgcttt ttacgagaaa atttgggata aacgatttct
     6481 gacatttttg gagcaataag ttgtttcttc gtttaaaaaa agcagaatgg atactggttt
     6541 atccattctg ctttattacg taaaatgaga aaataatcct gttctatttt tatgggataa
     6601 accatttatt ttttgtataa aaaatgtttg cgtttacata taattctcct agaaacataa
     6661 aaaggagtga atagcttgag aacaaaacaa gtattaaagg gagcgttggt catcttattt
     6721 gctgtaagta caagcgtccc gcaatggaca gtatatggag agagcatttc ttccgaatcg
     6781 gaatctcggg aggtagaaac cacagaaact actgatgctg gaaatgaaac gaaatcgagt
     6841 acttcacaaa cagaaacaaa tactgaaaca acggttgaga catctgttac tagcacagat
     6901 caagaaacga ccgctaccac agataaccaa caaacagaag aagcaacagc tacaaaaatg
     6961 tcaaacgacg aatcctcttc tgctaaagta agtgacaaag agaatgggac agaaaatgat
     7021 gatatcggta aaggtgaaaa tttcaatggt aaagaatatt tgaattactt cggtaaacag
     7081 atgacagaaa tacttaatgg accaacagaa gacatcaagt caaaattaat agagttatta
     7141 gtcactgagc ttgctgttaa tgattatggt gtagataaag agattatcaa acatctgatt
     7201 gctacttatc tattgtacga tatcgctttt gcaaaaaaca gtgtgttgat gtcgatgctt
     7261 ggaggtacag atgatgggac aacgcctaca gaaaacacag gcttctatcc aaatacaaaa
     7321 caacttcatc aaactgtact aaacaaacaa gatggcaaaa tgatggatgt atatgcatat
     7381 tatgtcgatc aaggttcaga taaaacagtc atgatccatg gcggatttcg agggaattgg
     7441 aataacggga tcgttacaga agaatacaat gatttttata aggccgggta taatttatta
     7501 tttgttgatt ccagagcgac tggtaatagc ggaggcgatt atgtgaccta tggacaatat
     7561 gagtcagatg acgtccttta ctggataaat caagaagttc gagagcggcc atctcaaaag
     7621 attttacttt atggtggttc gatgggagca gctacgatga tgtctgtatt agcgaaagat
     7681 attcctgtca atgtcaaagg aattattgaa aattgcggat ttgcatcaat tgatgaacaa
     7741 ctacgtttta cgtacagcca gacagtagcc cccgcactac cggatgccat aaaaaatcag
     7801 ttagacatca ttggggatca agagcatgaa gatttattta tgggcctttt gaaacaatac
     7861 tattttgatc aggagatgca tttggataca aaagctgctc tacctacgat tggaatgtca
     7921 gattcattgc ctaaattgat tatccatgga acagcagatg atgtagtacc tgtgtctaac
     7981 gcgcaaaaat tatatgagtt atcaggaggg tataaagatc ttttattagt agaaggagca
     8041 gggcatggaa aggcacagga agttgaccat gctgcctaca cgaaacatgt aacggacttt
     8101 ttgaaagtag tgttccatga tcagataaac gtaaagtatg tggatgaaaa caataaaagt
     8161 ctactcaaag accaggacga aatccagctt tacggagctt atggagaaaa ttatatgaca
     8221 gagcaaaaga cattcgaagg atacgaatta gcaaatgttg aaggaccgac tgaagggatt
     8281 tttaatgaga cgataccaac tattattttc tattacaaaa aaatcccagt agctccacca
     8341 aaaaagcaag atccgacaga gaatgcagac aataagggaa agaatgaacc tcgagaggat
     8401 caatcaagtg atgatacgca accgattcag atgacagtga aagtacaaaa aaagacagat
     8461 gcaaaaaaaa gaatgttgcc gaaaactgga gaaaagaaac attcgcttct catttattca
     8521 ggtggatttt tatctgttag cacgattatc acatggattt ggcgaagaaa gagagggtaa
     8581 atgaatgaaa tggttcaagc gaatagtgat tgcagtacca gttatcatcg tagttatttt
     8641 agtcactgta tttttgatta atcaaatcac accaaagccg attagcttgc ttgttagaaa
     8701 acaatttgac acaagtgaaa aagaacaagt atatgcccga ccagatgatt tccagcaaaa
     8761 aatcgaacaa atcgatataa aaaaagaaat atcctatcct tcaaagtaca aaaataataa
     8821 tatggatatc tacacaccga aagtggaaaa gaaaaaatta ccgattcttt tctggatgca
     8881 tggaggcgca tatgtaggag gggataaaaa tgattgtcgt gattatttgg aatatctatg
     8941 ttcagacacg cagcaaatca tcgtaaatat agattatgaa cgatcaccgg aagcaaaaca
     9001 tcctacacca gttatccaat tgaacgaagc cattcaagca gctaaaaaag agataaaaga
     9061 tcaagcagat tggtcgaata tttcgattgg tggaaattcg gcaggtgcac aaattagtgg
     9121 agaatatttg ttagcacttc aaaatgaaga aattaaaaaa gctgatcatc ttgatccgac
     9181 tttggagaac aagcagatta cgaagtttat ttctttgtct ggtctgttgg atccttctgc
     9241 acttactcaa gtaagtgata aaatctctag ctttctttat gaaaaatgtg gatgggctta
     9301 ctttgataat aaggactttg aacaatcaga gaaggtcaaa agtttagcct tggtcagaca
     9361 tgcggatcgt tggacgcaaa acacattctt aacggatgga aatacaaata cgtttacgaa
     9421 acaaatggaa gaagtaactt ctgtatttga gaaagcgggg gtgaaagtca caaaagtaga
     9481 ttatgcggag aaaaatgctg ttttgaacca tgaatatcag tttgatttca gcaataaaca
     9541 agcgcaagaa acatatcaaa agttagtaat gtttttcaag gagtaaagtc ttatgatgca
     9601 aaagaggttg taatctgtca cttagctcca agtcacaagg agcaatttca ataaacaagc
     9661 ttttcatggt ttatgaatat tgtaacttgt gactgaggag ttagagatgg caaggggcac
     9721 cttttattcg aaatttggga atatcacacg attattgtca cgatctcttt tacgtaaatt
     9781 ttataagtca gatttgttgt tttgatcgtt taaaaaaagc aacaaatccg aagcctgcgg
     9841 aggcaagaag ggacaaaaag ccgagtggta agcaaaagaa tgctggttcc actaatattc
     9901 cgttgttatt gatataaggc tgggaacaaa gatgaatagc aaataaaatc aatgcactag
     9961 cgaaaaaaag caaagaaata tggatagctt tcataagcag cacctccttt tttataattg
    10021 tatgataaat agattaagaa tctataaatg tgacaagttt gttaggatgt gaataaaatg
    10081 atcatctagc gaacttgaaa caaagatggc taatgataag caattaatga ttcagcaatc
    10141 agataggtac aaagtttacc atcatacgtc tagaccaata atagaaaata tgggaatata
    10201 atagaaaaga tttaggatac ccccaaaaga atttccccaa aaattcgatg tatcctaaac
    10261 ttttttattt tacataacaa tcatatcaac tgtgaaataa aattacaaat gataacctaa
    10321 aatgaggcca aaagccagtg ttatcaagga aatacttaat tcaccaaaat tttttttaat
    10381 tttttagaaa ataagaaaca aaaataataa aaaaaagaaa agataaattt tttactactg
    10441 tatactaact ttgttatcgc atctaacaag taactcgaat aataccaata caaccttctc
    10501 caaagatgcg atgacatacg ttctaaccca ccgactattg atgttctgta ccacccttgc
    10561 agaaccaaaa aagccactac caaatatttg gtagtggctt tttttgtttt atggattttt
    10621 cgctttttct gttatggtgc tattctttga tttcagaaat agtatgtcga actttagcga
    10681 tagaacgacg tgattctttt tcgttgtaaa acatcaaaat cacatataga gagtccaaaa
    10741 tgcttagttg agaaatacgg gaagctaaag cttcagatcg gaattctgtt tcttcagaaa
    10801 tagaaataaa aacgacatcg ccaagtttag caagggaaga attagcttgg ctagtaataa
    10861 cgattgtttt tgctccagtt tttcttacgg tttcagcaat atggattgtt tcttttgatt
    10921 gccctgtgtg agaaatcaaa acagcacagt ctttttccgt caataaagaa gcttccatca
    10981 attgaatatg gtaatccgtg ctgtgagaag ttgcgatagg ggaacgcaaa aatttatgat
    11041 aagcgtcagt tgctacaatc tctgaaccgc caacaccgaa aaaatagaca cgtttagatt
    11101 cattgataat agtagccgct ttttttagat ccttttgttg taacaacttt tttgtgtcat
    11161 ttaaagttga aatactagac tcgaacacct tttgcgccat agtgagctca gtatctgttt
    11221 tctgaatatt ttcgtggata gaaatagcag atagatcatt ttcttggatc aacatggcca
    11281 ttttaaaatc tttaaatcca ttataaccaa gtttttttgt aaattgaaaa aaagtagagt
    11341 cagcaatgcc cagttcactt gctaaattgc taataggact atgacttact ttgcttggat
    11401 tttctaaaat ataatcggcg atagcctgtt cttttgggct gaagtcgttg tacattgttt
    11461 tgatttgtag tcttatggag acgctcatga gaattcctca ttttcataat aagattatca
    11521 ttttttccgt tctcattaat gttaaagata caaggagagt aggaaaaaat caaacaaaaa
    11581 atatttttgt aaaatctatt tacaaaaaaa tttttttgta taatgagggt ataagaaatg
    11641 gaggcgctat catggaaatt ggtgtaattg gtttgggaaa aatgggattg aatttagctt
    11701 taaacttaaa agagcaccat cacaaagtac aaggactcga catttcaagt acagctgtta
    11761 gtcaggcaag agaaaatgac attcctgctt atcaagagat ctctgaattt ctagctactt
    11821 ttgagcagaa aaaaatcatc tggttaatgc ttcctgctgg agagataacc gaaaacatgt
    11881 taagaaaact ttttccaaaa ctcggaccag gagacattat tattgaaggc ggaaactctt
    11941 attataaaga ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc ggttacttgg
    12001 attgtgggac ttctggagga atcgaaggag cacgacatca agcctgctta atgattggag
    12061 gagataaaga aacttttcta tccgtggaac aattattcaa agatgtagca attgaaagag
    12121 gatatctgta tgctggtgca tccggaagcg gacacttctt gaaaatgatc cataatggaa
    12181 tcgaatatgg aatgatgcaa gcaatcggtg aaggattcca acttgtacaa aaaagtgagt
    12241 atgcttttga cttggaacaa gtcgcacgtg tttggaatca tggttcggtg attcgcggct
    12301 ggctgatgga aatcatggaa gatcagttaa acgaacatcc caatttaagt aattatcggg
    12361 gaatcgtttc tgcatcaggt gaagcaaaat ggacggttga aacagcgcta gaaatggctg
    12421 ttccagttcc agtaattgca actagtttgt tcatgaggaa tctttcacag gaagaagata
    12481 gtttttcagc aaaagtagtt tcagcactaa gaaatgggtt tggcggacat gaaatagttg
    12541 aaaagggtga gtaaaatgcc agattatcat atagcaatcg ttaattccag cagtttcgga
    12601 agagtctttc cacaccactt ggaaagatta aagtcagttg gagacgttca tttctttact
    12661 gttgatcaag agattccagg aaaagaacta gcggagttac ttaaaggatt caatatcatt
    12721 attgcaagtg ttacgccatt ttttcgacga gatttttttg aaaacaaaga tgaactgcta
    12781 ttgatttccc gacatggtat tggttacaac aatattgatt taaaagcagc aaaagaacac
    12841 cagacaatcg tatctatcat acctgcttta gttgaacgaa atgctgtagc agaaaataat
    12901 ataactaatt tgttgtccat gcttcgacgc acgacacaag cacaagcaag agtcaaggac
    12961 gatcgatggg aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt
    13021 gtgattggtg caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc
    13081 tgtgatgttc tagcttatga tccttacaaa tcagatttat acatcgaaag ctttggtgca
    13141 aaaaaagtag aacttcaaga attactggaa caatcagata tcatttgttt atgtgcgaat
    13201 ttgacagaag agaactatca catgatttct gataaagaag tcgatcagat gaaagaaaat
    13261 gtttatctat ctaatagtgc aagaggcgct ctagttgatg aagaagcgat gattaatggt
    13321 ctgaaaagcg gaaagatcgc aggatatgcc acagatgttt tagaagaaga accgggacgt
    13381 caagatcatc cgtatttagc gtttgaacaa gtagtgatta cgccacatat atcggcctac
    13441 acaagggaat gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga acagatcagt
    13501 cagcaccttc ttcctgaacg ttctgtccag acaaagagca actatataaa aggatgatga
    13561 agaaaatggt gaacggttta aaagtgaaaa caggccctca attttattta tatgaagaag
    13621 gtggtatttc aaaagtttct gatctcttga aatcatacgg cgcgaaaaga gtactggtca
    13681 cccacggaac ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa
    13741 cgattcaatt tttttatcat cgttattctg gagaatgtag ttatgctgaa gccagacgaa
    13801 tagctacaat catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact
    13861 gaccgatcta gtgggttata gtgccaatct tgcaagaatc gatttcggag tcatccctac
    13921 tttagctagt aattgtgctc catggacacc tcttgctgtc atgtataagg agaatggaca
    13981 agcagaaggg aaaacagaac acttttacag acaagctgct ttttttataa cagatcccca
    14041 attagtgatt gattcccctg ttccatattt cattgccggg ttagcagata cactagcaaa
    14101 atggtatgaa tctgagctca tcttagaaca agattttttg caagatgaat catttttgaa
    14161 gctagcacaa gatactgcga agatctgcaa agaagagatt ttgaatcatt ctgctaaagc
    14221 aatccaagat atggcacaaa gaaaactgac atcggagttc aaacagcttt cagagattat
    14281 ctttgctgtt gctggtttag ttggaggatt aggagataaa tacgcaagaa atgtggcagc
    14341 acatgctatg catgacgcta tcagtaaata tattccggaa agccatcgtt ttctacatgg
    14401 agaaaaagta gcgtatggga ttttctatca actggcacta gaaggtcgct gggaaaaaat
    14461 agacgagtta tatcctttgt atcgggaatt agaactgccg gtttctctag aacaaatggg
    14521 attatttccg aaagataaac agataataga taaaatggct gaatttattg attcgaaaga
    14581 gaaagtgcat ttgattccta tagaggtaac acagaaatca ctgatacagt caatgatgga
    14641 actggaaggt tatatcagaa agatctgaag gaggaggaca catggaaaca ttaagtgtag
    14701 tacaaagttt gttgattgct ttatgggtag ccgcggttat gtcccgctgg ttaggtggag
    14761 gagccacgtt gaccttacgt ttttctcctt tgatgactgg attagtcgtc gggttgatca
    14821 tgggggaagt tgcacaagcg atgattgtta cagccgcttt gcaaatgatt tatatggggg
    14881 ttttttctcc aggaggatca atgccagctg aaccatctat tgcagcagct atcgcagtac
    14941 cagtcgcatt attaggggat ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg
    15001 gattgctcgg aagttacttg tatcagtttc gttttttcat caatactttt ttaggtaaat
    15061 atacagacaa agctgtagaa gatttgaata caaaagctat ggcccgatca attattcttt
    15121 atccaacggt tgcctcattt ttattattcg ttccgcttgt tttcattgct ttatatttag
    15181 gggcgccatt gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt atccacattc
    15241 ttgaagtagt aggtggaggg ttagcggcaa tcggtatcgc gacaactgta tacgttatcg
    15301 gtcgaaaaga ttatctggtc tttttcttcc ttgcttattt tatgagtgtt gttttgaaat
    15361 ctttagatat tacgatggtt acttatgcca tttttgggat tattatcgct ttgatctttg
    15421 tccagtcgca aaaagggaaa caagtacagc ctgccacaag caataacaat tcagcatctt
    15481 ttgatgacga agatgactat gatgacggat tttaatttag gaggaatggg aaatggctga
    15541 aataatggat aatcaaacag gtgctcctga agaaatcacg aaaaaagatg taacaaaagc
    15601 ctatctgaga tggcattttg caaacgaaat tcctcactct tttgaacgtt acttggcgcc
    15661 atcactacta tacggaatga tgccaatctt gaaaaaactt tataaagatg aagactcttt
    15721 aaaagcagca tataaacggc agttactgtt cttcaacact caattaagct ggggtggcgg
    15781 tgtgattact ggcctaatgt cttctatgga acagcaaaga gcagaagaag agtatgaaga
    15841 aaaagagatc ttgatgcagg atgacttgat gtataacacg aaagcgggat tgatgggggc
    15901 actagcagga atcggagatg caatcgattc aggaaccgtc caatatattt ttatcgcaat
    15961 cgcggtacct tgggcacaac agggaagtcc tttaggcgca ctttttcctt ttatctgctt
    16021 tgggttgtat caggttttac taggggtatt tttcgcaaga caagctttca ctatgggccg
    16081 taatgctaca ggcctaaagc aaaacactgg tgttcagaca gcgattgaaa tgctatcagt
    16141 tttagggttg tttatgatgg gggtgcttgc gggaaactat gtaaaagtag aatcttcatt
    16201 gaaatttgcg atatccggac gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc
    16261 agggatatta cctttagctg tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt
    16321 gaaagtcaca caagcattat tatggctgac tggaatatta attgtgctag caggaatcgg
    16381 tattttataa aaagaaggga acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga
    16441 tgagcgtttg atccatgggc aagtaatggt aacgttatct caaaaaagtg gagtgaattc
    16501 catttttgtt gtagacgaag ttgtagcaaa agataaattt atgagagatc tttacaaaaa
    16561 tgcaggaagc cgtacaggac aaaaaacaat tgtcattaca ccagagaaag ctaaattcta
    16621 ttgggatgaa tatcaattta aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac
    16681 gatttacgac ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc
    16741 tcaaaaaaat cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga
    16801 tgctgaaaaa ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc
    16861 cactccatct gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata
    16921 aacaaatgct tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg
    16981 tgattgggaa tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca
    17041 gcgttttctt tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat
    17101 tgcttacgat acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc
    17161 agagaaagtt atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat
    17221 gtttgattgg aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt
    17281 gttgttcagt ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt
    17341 cgatctgcta tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt
    17401 attgggaaaa gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc
    17461 catattaacg atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt
    17521 tgttgatgaa ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa
    17581 agaggctatt cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg
    17641 aaattatttt tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa
    17701 cgtcaattat atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac
    17761 agatttacga gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg
    17821 ataagtaaaa agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac
    17881 acatggtaag ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag
    17941 ccatattgaa acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt
    18001 caaagaaaaa ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat
    18061 tagcgcaagt ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa
    18121 agcagccact tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca
    18181 tcagatgctt cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga
    18241 taatttaact gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta
    18301 gaaggaaggc tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga
    18361 tcccattaaa atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga
    18421 ctaaaatgcc tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg
    18481 agagccaagg actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg
    18541 cagggacaga tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt
    18601 ctcgatgtgg ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa
    18661 ctgatttgtt taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg
    18721 tcgatcagat ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca
    18781 aattgccggg ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg
    18841 aaggaatgac cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc
    18901 cagtgtgcga aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac
    18961 tttttggttt tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag
    19021 tagattctcc atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa
    19081 acgatttagt tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa
    19141 acgtggagtt tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct
    19201 cccttgatat gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac
    19261 gccctgatca tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg
    19321 atcgtgcaat gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac
    19381 cactgggaga gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta
    19441 ttagaaactg gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat
    19501 gaaatgcgta aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa
    19561 caaatgggca ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat
    19621 caatataaag aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa
    19681 gaaattttat cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca
    19741 cgggtaaaag tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc
    19801 atttcagggg atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt
    19861 tcaaaaatga atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc
    19921 gaagcagcaa ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg
    19981 cccgatcata atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa
    20041 gcagactatg aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg
    20101 tatctctaat aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag
    20161 ggaacagatg aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac
    20221 aatttagaac aaacacaaaa cgcatctact gttatctacc agtagatgcg tttaaagaga
    20281 agaacgagaa tcgtccatat gagggttttc
//
LOCUS       DACEPW010000036        20296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEPW010000036 DACEPW010000000
VERSION     DACEPW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.037"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1152
                     /locus_tag="IW819_001028"
     CDS             <1..1152
                     /locus_tag="IW819_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244147.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IW819_001029"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IW819_001029"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1244148.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IW819_001030"
     CDS             2728..3003
                     /locus_tag="IW819_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1244149.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IW819_001031"
     CDS             3009..3776
                     /locus_tag="IW819_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1244150.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDELLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IW819_001032"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IW819_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1244151.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IW819_001033"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IW819_001033"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1244152.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IW819_001034"
     CDS             7920..8222
                     /locus_tag="IW819_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1244153.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IW819_001035"
     CDS             8225..9388
                     /locus_tag="IW819_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1244154.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IW819_001036"
     CDS             9414..12842
                     /locus_tag="IW819_001036"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1244155.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IW819_001037"
     CDS             12863..13987
                     /locus_tag="IW819_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244156.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IW819_001038"
     CDS             13977..14414
                     /locus_tag="IW819_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1244157.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IW819_001039"
     CDS             14398..14736
                     /locus_tag="IW819_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1244158.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IW819_001040"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IW819_001040"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1244159.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IW819_001041"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IW819_001041"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1244160.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IW819_001042"
     CDS             15893..16936
                     /locus_tag="IW819_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1244161.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IW819_001043"
     CDS             17095..17823
                     /locus_tag="IW819_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1244162.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IW819_001044"
     CDS             17939..18184
                     /locus_tag="IW819_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1244163.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IW819_001045"
     CDS             18181..18399
                     /locus_tag="IW819_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1244164.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IW819_001046"
     CDS             18535..19074
                     /locus_tag="IW819_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1244165.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..>20296
                     /locus_tag="IW819_001047"
     CDS             19067..>20296
                     /locus_tag="IW819_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HAQ1244166.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRW"
BASE COUNT         6806 a         3502 c         4432 g         5556 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 tattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctgg
//
LOCUS       DACEPW010000037        19999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEPW010000037 DACEPW010000000
VERSION     DACEPW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.038"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..810)
                     /locus_tag="IW819_001048"
     CDS             complement(<1..810)
                     /locus_tag="IW819_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28"
                     /protein_id="HAQ1244167.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVS"
     gene            complement(822..2240)
                     /locus_tag="IW819_001049"
     CDS             complement(822..2240)
                     /locus_tag="IW819_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1244168.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(2480..4048)
                     /locus_tag="IW819_001050"
     CDS             complement(2480..4048)
                     /locus_tag="IW819_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1244169.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4052..4333)
                     /locus_tag="IW819_001051"
     CDS             complement(4052..4333)
                     /locus_tag="IW819_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1244170.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4330..5253)
                     /locus_tag="IW819_001052"
     CDS             complement(4330..5253)
                     /locus_tag="IW819_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1244171.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5250..6128)
                     /locus_tag="IW819_001053"
     CDS             complement(5250..6128)
                     /locus_tag="IW819_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1244172.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6115..7452)
                     /locus_tag="IW819_001054"
     CDS             complement(6115..7452)
                     /locus_tag="IW819_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1244173.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(7455..8558)
                     /locus_tag="IW819_001055"
     CDS             complement(7455..8558)
                     /locus_tag="IW819_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1244174.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(8632..10140)
                     /locus_tag="IW819_001056"
     CDS             complement(8632..10140)
                     /locus_tag="IW819_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1244175.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10106..10798)
                     /locus_tag="IW819_001057"
     CDS             complement(10106..10798)
                     /locus_tag="IW819_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1244176.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(10820..11794)
                     /locus_tag="IW819_001058"
     CDS             complement(10820..11794)
                     /locus_tag="IW819_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1244177.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12094..12966
                     /locus_tag="IW819_001059"
     CDS             12094..12966
                     /locus_tag="IW819_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1244178.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(13548..15746)
                     /locus_tag="IW819_001060"
     CDS             complement(13548..15746)
                     /locus_tag="IW819_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1244179.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            15978..16970
                     /locus_tag="IW819_001061"
     CDS             15978..16970
                     /locus_tag="IW819_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1244180.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17030..18451
                     /locus_tag="IW819_001062"
     CDS             17030..18451
                     /locus_tag="IW819_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1244181.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            18444..19943
                     /locus_tag="IW819_001063"
     CDS             18444..19943
                     /locus_tag="IW819_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1244182.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
BASE COUNT         6019 a         4121 c         3429 g         6430 t
ORIGIN      
        1 actaactcca gcactaactt cgctaccaat agttacaccg tatcctgaac ggattttaca
       61 tctagcaacc tcgattcttg atgattttcg attgactctt gcaccattgc catctctgcc
      121 agacttaata gcaatacaat catcaccaca ggccaaatca cattcataga cacgcacatt
      181 tgttgaagaa tcaatatcaa tcccatctgt acttggtcca tcattgttgc ggatgattac
      241 tttttctact aatacatcgt tagaataagt gatttgcaag ttccaaaaac ctgatttctg
      301 aaaaatcaaa tcacgaacaa caacatgttc tgcatgatac agtaaacaag cgcgtggacg
      361 tttaatagca taatccgcaa tccaacgaag tcccttttga tcgtattcag ctcttgtccc
      421 gcttttttga tcttttcccc aatacagctc ccaccaatgg tctccttgac cgtcgattat
      481 tccttttcct tctataagaa tatctttcgc cgagagaaca ttcaaaatcg ctgctggcca
      541 tttcatttct acgccggcta ctcgtgtatc gatttctgga aatgcttcga tatctgttct
      601 ccccttgagg actgcacctt cttcaaagat aagatgactc ttatctttta aaaaaagggc
      661 tccgactaaa taaatgcctt ttggaataaa tacaggtaag tgttctttcg caccttgatc
      721 gattgctttt tgcagtatta ttgtatcatc ttgttgagtg tcgtttgttt taaagttcaa
      781 agcattgata atcccttttt ttgtccgcaa tacgtttctc cctatctttc attgattttt
      841 ttcaattctt caccaataaa tacagttttt gaacgattca gtgggtaacc tactagcaaa
      901 atgattgcgg atacaaataa gcagaatgct ggtaacaagt ttgccatcgt ataaatcctt
      961 tcttgtacta aaactgattg gatggctcca cctgttgtag acgattggta accaatgagt
     1021 gccagcataa atccgcctac cgctcctgca catgcttgtc ctacttttct cgcaaaagaa
     1081 tttactccat atactgttcc atcttctctt aagcctgtct tatattgatg ataatcaatc
     1141 acgtcagtaa tgaatgccca gaccatcaaa ttgaataaac cagatcctaa agtagcgata
     1201 aataaaagaa tcagatatac ccatgcattt gttatatgaa ggaaatacat cagcaagtac
     1261 atacatgaag aagcaagtaa cgcaaatacg cttgcttcct ttttaccaaa taattttgtc
     1321 agctttgtag caaatggtgc taaaactact actgttccgt aagtaaataa aagagcaaca
     1381 gacattgctt gtttattatg gaagtaatca ttgaataaat aagtcatatt cgtcccagct
     1441 aaaatctgat tgatcacaat gaaaatgtca acaatgacta aaataatcag tgctcgatta
     1501 gctaacaaac cttttaccaa ctgagaagca gatacttttt cgtttttgac aactcttact
     1561 ctttcagtag tcaatttaca agttaacata tatgcgacaa atgctattgc agcacatccg
     1621 attgaaatcc aaaagaaatg attccctgaa acgacttgtt gtccactctt gttatgggta
     1681 tacattaaaa tcggaatgaa gaatcctgta gttgatccgc caatcgcaga accaatgctt
     1741 cggaacgtgg acaacgaggt gcggtcatta ggatttgagc taattgctgc tgccattgct
     1801 ccatacggga tattgattgt tgagagaaaa atcccataaa caatgtatga aacaaaaata
     1861 taagcaatct tcaccgtcat tggaagatgt tggacgaatg gcaaaaacaa aatgacagtc
     1921 actaagcaaa atggatactt catttttctg atccaaggaa taaatcggcc agtttctgtt
     1981 aactctgaga catcacataa tcttccaacc atcacatcag caaacgcatc caaaattctc
     2041 gaaatcaaga ataacgtacc tacgattgcc cctgaaacgc caagaacatt cgtataataa
     2101 atcatcaaga aactattgac taaacctaaa ataaaacagt tacctagatc accgaacatg
     2161 tagcctatct tatcggaaat cccaaattcc tttttttctt tttttactgt atttgctgta
     2221 acagcatttt gtgaaagcat ttgaattcct cctacctctt ctagtaaacg ttttcaaaat
     2281 tataactctt tttttcttgt ttttatattc acaatgttgg ctttttattg cattatcttg
     2341 acagataaaa aacaaaaaat aatctaaaaa cattgatgca taagattgat aacttttttt
     2401 acataacaat atatacaaac aaaaaaattc actaactagt gaaaataagt tagtgaacct
     2461 catttattaa agaattaaat tacagatgat cttttccatc atacttgaaa aactcaaaat
     2521 cagcataact tcctcttttt ttatttagat catgcactgc tatcccgata aaatttccag
     2581 taaaccctcc tgagagtata cacatgtttt tttgcggacc tactttcttc cattcttcag
     2641 ttgatacgtc tttcaaatga aactgtgctg tctctttctt tacagtgact ttcaaaaaag
     2701 tcttcttttc tgtgagtgga atcgtccaat cttctatttg tgctactccg ttttctgatt
     2761 ctatcatacg taggcatttt cccttgtcct cattccaagt aacataacaa taaatataat
     2821 tggattcatt taagtacagt accagtccag ccatctgatt aaaattatta ggcatgtatt
     2881 caaatgctgt ctccgcatca aaagtaaaat ccgtttgccg aatagctaac aaatgatgtt
     2941 cgaaggtaga ttgaagagat tcaccggaaa tcagacgtaa aaaacctggt ctacattttg
     3001 tatcacacca atcagcattt ggcaaaatgc gtctggcatt ccagttcttc tttagctctc
     3061 cttcaaatgt atccataaac tgatgatctt tttcttgttg tacctcttca tttgtctcaa
     3121 tcattatttc ttccatcgga gttgtctggc tatctttaag tcttaaccaa tcgtcggacg
     3181 tccaatacac ttcttgaata gcggtttctc ttcccaaaat agccgcaccg tttaatggtc
     3241 tggtacaaag atatgtcatg taccatcttc ctgacggggt ttgtactaag ctcccatgtc
     3301 ctgtacattg caatgtagaa gcaggtttgt cactagctgt cagcatagga aaaccaggat
     3361 caagctcgta aggaccagta atattctttg ctcggcaaac tgtaactgaa tgtcctcttc
     3421 ctgtaccgcc ttctgccgtc atcaaataat aatacccgtt acgaaaatac aaatgtggtg
     3481 cctccgtttt cgctaattct gtacctgcaa atattttata gatgggacca tcaagcttct
     3541 tcttctctgc atcatattct tgtaatacga taccgctgga tttattcggt gtgtccagac
     3601 gataatccca tagttcattg acaagccatt tttttcccgt ttcaagatca tggaacaaag
     3661 aaggatcaaa gccgttacta tttaaataaa ttggttcgga ccaaggacct gtgatctcag
     3721 atgcagtgat caaataattg tggacatctt taaacggacg gcctgcactt ttgacatctg
     3781 tgtaaacaag ataaaacaat ccatcagcat aactgatctg tggcgcccat atacttccat
     3841 ttgtaggatt tccttgcaaa gatacttgag tggtcaaaat atccgttgta tgtgaccagt
     3901 tgactaaatt tttcgactca tatactcgga ttcctggaag ccattcaaaa gaggagacaa
     3961 taatgtaata ccgctctttc actcttaata tattaggatc tggattgaat cctcttaaaa
     4021 taggattttt cactttgata gtctgcatga ttcaggctcc ttttctcatt cttactcgat
     4081 ataaactgaa tgccccagct gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     4141 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     4201 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     4261 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     4321 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     4381 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     4441 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     4501 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     4561 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     4621 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     4681 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     4741 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     4801 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     4861 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     4921 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     4981 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     5041 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     5101 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     5161 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     5221 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     5281 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     5341 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     5401 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     5461 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     5521 ccatgatgat ctaggcggcg atgtagcaaa agtgtttcga tgtccacctg tgccgctaga
     5581 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     5641 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     5701 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     5761 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     5821 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     5881 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     5941 aggtaaagga ccgtcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     6001 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     6061 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     6121 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     6181 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     6241 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     6301 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     6361 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     6421 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     6481 gaatgctgat gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     6541 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     6601 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     6661 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     6721 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     6781 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     6841 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     6901 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     6961 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     7021 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     7081 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     7141 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     7201 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     7261 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     7321 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     7381 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     7441 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     7501 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     7561 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     7621 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     7681 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     7741 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     7801 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     7861 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     7921 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     7981 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     8041 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     8101 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     8161 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     8221 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     8281 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     8341 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     8401 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     8461 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     8521 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     8581 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     8641 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     8701 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     8761 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     8821 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     8881 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
     8941 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
     9001 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
     9061 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
     9121 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
     9181 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
     9241 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
     9301 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
     9361 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
     9421 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
     9481 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
     9541 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
     9601 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
     9661 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
     9721 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
     9781 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
     9841 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
     9901 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
     9961 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    10021 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    10081 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    10141 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    10201 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    10261 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    10321 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    10381 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    10441 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    10501 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    10561 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    10621 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    10681 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    10741 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    10801 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    10861 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    10921 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    10981 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    11041 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    11101 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    11161 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    11221 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    11281 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    11341 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    11401 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    11461 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    11521 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    11581 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    11641 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    11701 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    11761 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    11821 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttatc
    11881 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    11941 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    12001 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    12061 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    12121 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    12181 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    12241 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    12301 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    12361 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    12421 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    12481 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    12541 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    12601 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    12661 ccattagctt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    12721 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    12781 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    12841 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    12901 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    12961 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    13021 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    13081 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    13141 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    13201 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    13261 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    13321 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    13381 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattaaacgg cgtttatctt
    13441 gttgattctg cggcaataaa atcagccagc agcaaaacat gaagtgctgt tttttgctgt
    13501 cctcttttat tgcttgaatc agatcacaag aaaaacagcc tcttgattca caaatatttt
    13561 tttcgatatt ctcccggcgt aatcgattcc ttctttttaa agaaacgaat aaaattttgc
    13621 ggatttcgat attgcaatct ttctgcaatc tcttttacgg agaggttagt ctctttcaac
    13681 catttttttg ctacctctaa acgatgattt tgaacaaaat caccaaattt ttctcctgtt
    13741 tcctttttaa atacattact taaatagtta gggttataat gcaacctatc agcgatcaca
    13801 tctaaagaca gatcacgatc atattctgtg tagacgatat gcataatatt ctccgacaaa
    13861 tttttaaatt catgatcagt gattgcttga gagcattctg tgattggcag aactaattgt
    13921 tctaaaacaa gtttttctag ttctttggga tgataattgt tcagtgcttt tacataaatt
    13981 tgtttcatgt tatcaaagtt ttttgaatct actcccaata actgtcctaa ttgaacgagt
    14041 tcattgatca agcggaccga aactacttcc cgactcaaag gatttttatt ttgtgcaaac
    14101 aatgcatcaa tcaattcatg tactaaattc ttcacttcct gatttccaga acgaatggct
    14161 tcaaacaatc gattttgttc ttctatcgga tatctaataa gtgttttttc atgagcagca
    14221 ggaacgattt cctgataaaa aataattgaa cttggaccag tatttacacg ataatacagg
    14281 gcttcttttg cccgatcaac cgattgcttg ctttcattca gcgtttcgaa ccggttactg
    14341 atgcctatac tgatagttaa attcaagtat tcttttacac gtttttgaat gagtcggcaa
    14401 tactccatga cttttttgct tgcttctgca tcatctttat ccattcgata aatagtggct
    14461 tgcatttctt cattcaaaac gatcggcagc attcgatttt cctttggcac aatttccgtg
    14521 atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc caccgtgact atcttctaac
    14581 agatcgattt gaaccaaagc tgtataatag cagtcatttt gagaactata accaaattgc
    14641 tgcaaccgct gatctagttc tcgcctttct actcgatttc taaataaact aagtacaaat
    14701 agcgtttcca gctgtggctt ttgggtaact aagtgtgcac ttaacgcttc tttttctcct
    14761 atgatccgac tgactgattg agcgaccaaa tctagttctt tcccactgaa tccttttgat
    14821 ttaagattca gacgttcctg tatttgagaa atcggacggg caaatctgtc cgaaaataag
    14881 taggaaaaaa gaccaatgag tcctaccagt gttaaagaca ctgcaactag cccgattctc
    14941 agattgcgga tcattgcgcc aattgcacta gagtcaactt ctaatctgta aatccaatta
    15001 ttataatctg atttcagtac tatatacgtt ttatcttttt ctcttatcac tttcagctga
    15061 ttgtaatcta gatccacgtc cgttaataca gttggttgct ctttattttt tactgtctga
    15121 gtagtaaaaa ggttgccaac ttcgttttca atagaaagta tgtcttctcc actttcacca
    15181 acaacttgtt ctattgtatg tttctctatc gtagcaattc ccaatgcata tttttctttt
    15241 tcaaacattg gcaaagaaat caccatttcg attcctttgt ttttcttttg ccaaaataaa
    15301 ttatttttgt tatttatgta cttctgttga tacttttcaa cttcctcttc ggtcagttga
    15361 ctcaacgatc cattgattac tccccattta ccagccaaac tgactaaatc ataattatta
    15421 tcatccataa caatcgtttc tataaaattt agttctttcc tgatttcaga ataagtggag
    15481 taatctgtat atgtcagcgg atgcttgaat atgtccgaga aatttttggt cgaaccatac
    15541 gtagaaaacg aatattcaat cgttctaatt ttccattcaa cgttagtttt aacttgcctg
    15601 agcaaattct ttttgtctgt taaataactc gattcgatcg attcataggt attctggtaa
    15661 atgaataaac taaatcctat gacaagtacg gctcctaata aaaacatcgg taaaaatatt
    15721 ttataaaata ctgtactttt cttcattttc ttcctctcct ccttttaaaa taaaaggctt
    15781 tcatttttct gtaaaattga tactcatcta attaaatgag cattccccgc tttcaagaat
    15841 agcataatta aaaataaaaa tccaagtaat ttttaacgtg tgcaatcttt tcatatcaaa
    15901 gatctattga cttatcgtag ttatcgtaat atgatgaatg cgttaacaaa aagtgaaaaa
    15961 gtaaggaaaa tgatggaatg gtaacgatca aagatatcgc taaagcagct ggcgtttctc
    16021 atacgacagt ttctcgtgct ttgaataaca gcagtttaat aaaaaatgaa acaaaacaaa
    16081 aaatacgaca actagctaag gaaatgaatt attcgccaaa ctatagtgca aaaggtttag
    16141 tgaatcaaaa aaaatatcta atcggactat ttttctcaag tttgcataaa ggaacttcat
    16201 caaatttcct agcagattcc attgcaggca tccattcagt attagacaca aattacagtt
    16261 tatctgttga aagtatcgat gttgttgatt taacagaaat caacctgcaa cgctacgatg
    16321 gactgatcgt tatgagtcaa agtgatgatg atcagccttt catcgactat ctcaaagaaa
    16381 aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga tccagcggtt atcaacgtca
    16441 ctgccgatga tgcggccggt gttatgcagg cagtagatta cggtattgga tgcggacata
    16501 cgaagatcgc ttatattggc ggaaagaaaa attttcgttc gacaaaagaa cgggaaaaag
    16561 gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa tctagcctat tttttaacag
    16621 gagattacag cttagaaagc ggcctccaaa acatggaata cctattatca ctttctgacc
    16681 tgcctactct ggttttttgc ggcaacgacg acatagcgat tggtgcattg agagctgtac
    16741 atttagctgg cctccgtgtt ccccagaaca tttctctgat cgggttcgat gattctccag
    16801 tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa gcctttgaaa gaaatgtgtc
    16861 aagaaggaac aaagctttta ctagatttga tcgaaggcag gacgatcgaa caatctaaaa
    16921 ttgagctctc caccttgttg atccttcgtg agtcgatcaa ttacttgtaa actacaagta
    16981 attttatata aaaatgttaa cgtgtgcata acacagaaag gaagtttata tgaaaaaact
    17041 gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa gttcttcagt ttggtgaagg
    17101 caattttatg cgtggattta ttgattggca gattcagcag ctgaataacc aacaattgtt
    17161 taaaggtaat gttgcaatca ttcaaccttt agaacaaggt cttggaaata tgatgaacca
    17221 gcaagataat ttatatacgg tcattttaca aggactcagc aatggcgaaa tcgttgatac
    17281 ttccgagatt atcaccgtag tagaacaaat gatcaatcct tatgatgaat gggatgctta
    17341 tctatctcta gcagaaaatg acgacttggc ctatatcgtc tccaatacaa cagaggcagg
    17401 aatccaattc gtggagactg ataatttaac agatactccc ccacagagct tcccgggcaa
    17461 actgacagcc tttttatacc gacgtttcaa attaaacaaa gctggcttta ccatcattcc
    17521 ttgcgaactg attgacagaa acggcgaacg tctaaaagaa atcattttac gctacgcaga
    17581 aaaatgggat ttagaacctg cttttgttga gtggttagaa aaagaaaatg ttttctgttg
    17641 tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa gatgcaggtg ttttcaatca
    17701 acggcataac tatgaagatc aattgatggt caaagcagaa ccattcatgc tttgggtgat
    17761 cgaagggcct aaagaattgg agaatcaact accattcaaa caggctggct tgaatgtcat
    17821 catcacagat gatatgactc cttatcgtga acgtaaggtg catttgctta atggaccgca
    17881 tacagcgatg gtccctcttg ctttgttggc ggaattggaa actgtagaag aagtaatgaa
    17941 agatccttta ttctccgcat atgttgatca gttattcaat ctagaattga ttcctatgct
    18001 ttctttacct aaagatgaat tagccattta tgctgaccaa atcaaagaac gtttcttgaa
    18061 tccttttgcc catcacaaat tagaagcgat ttcattaaat agtgtttcaa agttcagcac
    18121 ccgtctgctt ccagtgttca agaagtatat cgaagaacaa aatcaggtac caccgttaat
    18181 cactgtttca ctagctgcgc tacttcttat gtatcgaggc gatcaagtaa agccacatga
    18241 tgatgaaaag acgataagcg aatttactga tgcttggtct gataatggaa cagctattcc
    18301 tcgattattg caaaacgctg cgctttgggg agaagatttg tctcaaatac cgaatgtgac
    18361 agatacagtt caagaaatag ctgcaaagat cgatcaaaaa ggtgtccggt cagtattaca
    18421 agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca accaacaatg aaattaaatc
    18481 gacgagacag tgtggtcgtc gcattaaccc ctattccaaa acaaacacaa ctgacgattg
    18541 atcagcaaac gatcaccact cttgaagata tcccgcaagg tcataagatt gcattgggtg
    18601 atttgaaagc tggagacaac gtgatcaaat atggctatcc aatcggtcat gtgacgacag
    18661 acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa aaccaatctt tctggtgaat
    18721 tggattatcg atatgaaaaa gatgtccatc caagtcatta cctttttgag aaccgaacat
    18781 ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg taatgatttg tttttagtac
    18841 ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt caaacagttc aaggaaaaac
    18901 atccagatct gggacagttt gaccatatta cgattttaaa gcatccttat ggctgttcgc
    18961 agcttggaaa agatcatcaa aatactcgag aaattttagc ggacgcagta aatcatccca
    19021 atgccggagg agttttagta tttggattag gttgtgagaa caatacagtt cctgaattca
    19081 aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt tctagtggct caagatgttt
    19141 acgatgagat cgaacaaggt gtggctctat tagaagagct tttaacagcg gcggaaaatg
    19201 accagcgcat atcagtcccg ctctctaagt tgaatgtcgg tttgaaatgc ggcggttctg
    19261 atggtctatc gggaataact gctaatcctt tacttggtgc attttctgac tacttgattg
    19321 ctcaaggagg cagcacgatc ttaacagaag tgccagaaat gtttggcgca gaacaagtat
    19381 tgatggcacg agcggaaaat gaagaagttt ttgactcaat cgttgacttg atcaatgact
    19441 tcaaacagta tttcttgtct tacggggaac cagtttacga aaacccatct cctggtaata
    19501 aagcaggtgg gatcactaca ttagaagata agtctttagg ctgcacgcaa aaatcaggaa
    19561 gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat cagaaaacca gggcttagtt
    19621 tattgcaagc tcccgggaat gatcttgtag ctgcctctgc tcttgcttca tccgattgcc
    19681 agttggtctt gtttaccaca ggaagaggga ctccgtttgg ctcatatgtc ccaacggtca
    19741 aggtatctac gaatacaact ctttttgaac gcaaaggtca ttggatggat tttaatgcag
    19801 gcgtcttatt agaacaacca atggaagtaa tcttagaatc gtttgttcaa aaaatcatcg
    19861 ctgtagctag tggagaagaa acgaaaaatg aacaaaatga cgttcgagaa attgccattt
    19921 ttaaaaacgg agtaacactt tgattttcat aaaacagtat aaaaagaaag aaggaagatt
    19981 tatgttttta tctgatgat
//
LOCUS       DACEPW010000038        19456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEPW010000038 DACEPW010000000
VERSION     DACEPW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.039"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            132..3659
                     /locus_tag="IW819_001064"
     CDS             132..3659
                     /locus_tag="IW819_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1244183.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="IW819_001065"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="IW819_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1244184.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="IW819_001066"
     CDS             7447..7788
                     /locus_tag="IW819_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1244185.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="IW819_001067"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="IW819_001067"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1244186.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /locus_tag="IW819_001068"
     CDS             9145..11565
                     /locus_tag="IW819_001068"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1244187.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IW819_001069"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IW819_001069"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1244188.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12999..13736
                     /locus_tag="IW819_001070"
     CDS             12999..13736
                     /locus_tag="IW819_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244189.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13750..14565
                     /locus_tag="IW819_001071"
     CDS             13750..14565
                     /locus_tag="IW819_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1244190.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14578..15225
                     /locus_tag="IW819_001072"
     CDS             14578..15225
                     /locus_tag="IW819_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1244191.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15241..15897
                     /locus_tag="IW819_001073"
     CDS             15241..15897
                     /locus_tag="IW819_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1244192.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQSTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16059..16880
                     /gene="racE"
                     /locus_tag="IW819_001074"
     CDS             16059..16880
                     /gene="racE"
                     /locus_tag="IW819_001074"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1244193.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16883..18238
                     /gene="rph"
                     /locus_tag="IW819_001075"
     CDS             16883..18238
                     /gene="rph"
                     /locus_tag="IW819_001075"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1244194.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18239..18757
                     /locus_tag="IW819_001076"
     CDS             18239..18757
                     /locus_tag="IW819_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1244195.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18864..19364
                     /locus_tag="IW819_001077"
     CDS             18864..19364
                     /locus_tag="IW819_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1244196.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6540 a         3341 c         4179 g         5396 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12961 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    13021 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    13081 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    13141 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    13201 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    13261 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    13321 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    13381 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    13441 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    13501 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    13561 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    13621 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    13681 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13741 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13801 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13861 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13921 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13981 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    14041 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    14101 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    14161 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    14221 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    14281 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    14341 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    14401 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    14461 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    14521 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    14581 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    14641 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    14701 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14761 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14821 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14881 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14941 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    15001 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    15061 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    15121 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    15181 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    15241 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    15301 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    15361 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    15421 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    15481 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    15541 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    15601 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    15661 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15721 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15781 cagagcacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15841 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15901 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15961 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    16021 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    16081 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    16141 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    16201 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    16261 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    16321 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    16381 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    16441 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    16501 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    16561 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    16621 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    16681 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16741 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16801 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16861 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16921 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16981 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    17041 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    17101 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    17161 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    17221 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    17281 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    17341 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    17401 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    17461 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    17521 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    17581 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    17641 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    17701 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17761 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17821 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17881 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17941 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    18001 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    18061 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    18121 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    18181 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    18241 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    18301 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    18361 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    18421 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    18481 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    18541 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    18601 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    18661 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18721 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18781 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18841 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18901 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18961 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    19021 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    19081 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    19141 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    19201 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    19261 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    19321 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    19381 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    19441 aggctgggac aaaagc
//
LOCUS       DACEPW010000039        19232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEPW010000039 DACEPW010000000
VERSION     DACEPW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.040"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            912..2162
                     /locus_tag="IW819_001078"
     CDS             912..2162
                     /locus_tag="IW819_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1244197.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2181..3191
                     /locus_tag="IW819_001079"
     CDS             2181..3191
                     /locus_tag="IW819_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ1244198.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDVHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFEDGGTRVIHYTPETYIEKQ
                     NG"
     gene            3201..3854
                     /locus_tag="IW819_001080"
     CDS             3201..3854
                     /locus_tag="IW819_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1244199.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3873..4871
                     /locus_tag="IW819_001081"
     CDS             3873..4871
                     /locus_tag="IW819_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1244200.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWKPTVSLEEGLRESYNWIEKQIKG"
     gene            4947..5708
                     /locus_tag="IW819_001082"
     CDS             4947..5708
                     /locus_tag="IW819_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ1244201.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5726..6832
                     /locus_tag="IW819_001083"
     CDS             5726..6832
                     /locus_tag="IW819_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1244202.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRDVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6822..7055
                     /locus_tag="IW819_001084"
     CDS             6822..7055
                     /locus_tag="IW819_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244203.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            7254..7709
                     /locus_tag="IW819_001085"
     CDS             7254..7709
                     /locus_tag="IW819_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ1244204.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7723..8496
                     /locus_tag="IW819_001086"
     CDS             7723..8496
                     /locus_tag="IW819_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1244205.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8483..9613
                     /locus_tag="IW819_001087"
     CDS             8483..9613
                     /locus_tag="IW819_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1244206.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9644..10879
                     /locus_tag="IW819_001088"
     CDS             9644..10879
                     /locus_tag="IW819_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244207.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFVPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLSVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10904..12013
                     /locus_tag="IW819_001089"
     CDS             10904..12013
                     /locus_tag="IW819_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244208.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAKYEKYMDFVWEE"
     gene            12055..13389
                     /gene="gmhB"
                     /locus_tag="IW819_001090"
     CDS             12055..13389
                     /gene="gmhB"
                     /locus_tag="IW819_001090"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAQ1244209.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13424..15061
                     /locus_tag="IW819_001091"
     CDS             13424..15061
                     /locus_tag="IW819_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1244210.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            15163..15558
                     /locus_tag="IW819_001092"
     CDS             15163..15558
                     /locus_tag="IW819_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1244211.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15576..16307
                     /locus_tag="IW819_001093"
     CDS             15576..16307
                     /locus_tag="IW819_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244212.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            16331..17782
                     /locus_tag="IW819_001094"
     CDS             16331..17782
                     /locus_tag="IW819_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1244213.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            17808..18419
                     /locus_tag="IW819_001095"
     CDS             17808..18419
                     /locus_tag="IW819_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1244214.1"
                     /translation="MIDIISAIMVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            18601..19116
                     /locus_tag="IW819_001096"
     CDS             18601..19116
                     /locus_tag="IW819_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAQ1244215.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNGERLSF"
BASE COUNT         6194 a         2684 c         4207 g         6147 t
ORIGIN      
        1 atatatatgt atatggtgac tgaagacaaa aaaacagaac taatgaaact gaataaaaat
       61 atagagtgaa ctagtttcta agagggattt taaactagat ttcattggta ataaaatttg
      121 gagagatgga aaaacagcac ttaaggaagc agattttgtt gtcatttttg ttcctacaaa
      181 ctatggtagt gaatgtgtta ttgaattagt gatgtctgtt atttcagagg cattggggat
      241 aattaaatcc actatacctg taggatatac aaaaagtgga agaaaaaaat attatcaata
      301 gattttattt agttcagaat ttttacgtga aagtagatta ttaatgataa tctttatcca
      361 attagaatta ttatcgtagg atgtgatgat gaaagccgtg aaattgttca agtatttacc
      421 aatgtctccc tgccagtaaa tccgcttaga atttgagcct tgaaaatgtg caatgataaa
      481 aaacaaaaac tcgctcaaaa tagacagttt ggggtgggaa tagaccaata atgtaccaga
      541 caaacttcca tttggggtga aaataggtca ataaagcgat gttaaacata cctttggagt
      601 aagaatagag ggtaataagt gaatttataa actttttggt gactggttga gtggaaaatg
      661 taggtacatt tcaatgcgaa aatgtattat aaccccaaac gaggtcaata aaaatgactc
      721 aaacttgcaa tataacccta aacgggtagg ggatttatga aaaattgaat ttgccggcgt
      781 aggagctggc tgttttaagt atgaatgaac acagctttcg ggctgtttta tataaatagc
      841 aggttactgt tcacaaacag cattttgtta agaaagtagt gaccacagca gtggtggaaa
      901 gaggaaatat tatgaagaaa cagtataaca ttgcagtcgc cggcaccggt tatgttggtt
      961 tgagtattgc cactctgttg gcacagcacc ataaggttac agcggtggat atcattccag
     1021 agaaggttga actgattaat aaccgtaaat ctccaatcca ggatgattat attgaaaagt
     1081 acctggctga gaaagaactg aatctgaccg caacattgga tgcaaaggca gcataccagt
     1141 ctgcggattt tgttgttatt gcggctccta ctaactacaa tagccgcact caacattttg
     1201 acacatccgc tgttgaggct gttattaggc tggtcatgga agtaaatcct gatgccatta
     1261 tggttattaa gtccaccatt cctgttggat acaccgcttc tgttcgtgta aagactggta
     1321 gcaagaatat tattttcagc cctgagttcc tgcgtgaaag taaggcactg tatgacaatc
     1381 tgtatccttc gcgtattatt gtgggtaccg atatggatga tgagcgtttg gttgaggatg
     1441 cacacacttt tgctacactg ctacaggaag gcgcaatcaa agagaatgtg gacacattgt
     1501 tcatgggctt tactgaagca gaagctgtta agctgtttgc caatacatat ctggctcttc
     1561 gtgtcagcta cttcaatgag ctggacactt atgcagagat gaaaggcttg aacactcagc
     1621 agatcattaa tggagtttgc cttgatcctc gtattggtac tcattacaac aacccttcct
     1681 ttggttacgg tggctattgc ctgccgaagg ataccaagca gcttctggcc aactacgcag
     1741 atgttcctga aaatctgatc gaggcaatcg ttgaatctaa ccgtactcgt aaggacttta
     1801 ttgcggatcg tgtacttcag atggctggtt attacaatta cgcagaagca aatcagtatg
     1861 atgctggcaa agaaaagcct gttgtgattg gtgtttaccg tttgaccatg aaatccaatt
     1921 ctgataactt ccgccagagt tctatccagg gcgttatgaa gcgtgtaaaa gctaaaggtg
     1981 caactgttgt gatttatgag ccgaccttga aagatggaga gactttcttt ggcagtaagg
     2041 ttgtgaatga tttggaacag tttaaggcaa tgtgtgattc tgttatagca aatcgctacg
     2101 atgcttgcct tgatgacatt aaggaaaaag tatatacccg tgatatcttc cagagagact
     2161 aagattagga gagagtattt atgattatta caaaaacacc gtttcgtatg tctttttttg
     2221 gcggtggaac ggatatggag gattatttca aagagaatgg cggtgcagtt ctttctacta
     2281 cttttgataa gtactgttat gtgaatgtga gacatctgcc tagatttttt gattacagcg
     2341 ctgagttatc ttattctcgt actgagagag taactagcat tgaagatatt gagcatcctg
     2401 cgattcgtaa tgcgatgaaa atgctcgatg tgcatgaaat tcggcttaca tacgaagctg
     2461 atttacctgc tcgctctggt ctgggtacat ccagttcttt tgcagttggt atgttgaatg
     2521 ctttttatgc actggagggt aagtatgctg acaagaagaa acttgctgat gaagccattt
     2581 atcttgaaag agttctgtgt aatgaagccg gtggatggca ggatcagatt gccgcatctt
     2641 atggtgggtt caatcgaatt aatttcaatg tggatggtta tgaggtgctg cctgtcatta
     2701 tttcgcctga acgtaaaaag cagcttaaca acaatctgat gatgttcttt accggattca
     2761 ctagattttc ttctgatgtt caaaaggcta acaacgtcag tggcacagag gataagagag
     2821 taagactgaa aaagatgtat gagcttgtag atgaagctga ggcaattctt actgataaga
     2881 acagaaattt agatgatttc ggtagacagc ttgatgttac ttggagattg aaaaaaggta
     2941 ctggtggagc aattagtaca ggaagcattg atgaactata tgaaaagggt atggctgcag
     3001 gtgctttagg cggaaagttg ctcggtgcag gtggtggagg tttcttggtt ttctatgtac
     3061 aacctgagaa acaggatgca gttcgttggg ctatgcgtga tttgatgtat attccgtttg
     3121 aatttgaaga tggtggaact agagttattc actacacgcc ggaaacttat attgaaaaac
     3181 agaatggata aggagtaaca atgttagcgg aaaaacttaa taaacatatt gacttattga
     3241 ttaaacgata tcctacactt gagacgatta aacaggacat tatcgatgga tacctaatta
     3301 tggaggaatg ctatttgaat ggtggtaaac ttcttgtggc aggaaacgga ggatcagccg
     3361 ctgactcgga acatattgct ggcgagttga tgaagcggtt taagacgcca cgtcctgtaa
     3421 gtaaggaatt tgccgataag ttagttgcaa ttgaccctgt aagaggtccg gaattagcca
     3481 aaaatctcga gtgtggttta acggcaattc ctttggtcgc acacgaagca ttgtctactg
     3541 cttatattaa tgatgtcgat ggtttgggtg tcttcgcaca gcagctgttt ggctttggta
     3601 gatctggtga cgtattcctt ggtatttcga cgtccggaaa tagcaagaat attatgagcg
     3661 caactgttgt tgctagggct ctcggtataa aagttattgg cttgacgggc gcaaagggtg
     3721 gggaacttgc cacggtagca gatgtttgtg ttaaggctcc tgaagttgaa acatacatga
     3781 ttcaagagta tcacttgccg atatatcatt gttggtgttt gatgctggaa gataaattct
     3841 ttggaaacga ataaaatttg gaggaatgtt aaatgaaaaa gacaattatg gttaccggtg
     3901 gttgcggtat gattggctcc aatcttgtta agagactggt gaaggaaggc tgctgggatg
     3961 tttatgtagc tgataatctg tggagaggaa agttggagta cctgaacgac gaagatgggc
     4021 atcctgtcat cgacctggat actcatttct tcaatgctga tctgactgat tatgagcagg
     4081 ctaaaaaggt tattggtacg actgagtatg tagttcatct ggctgatgtt gtggctggca
     4141 ttgactatgt gtttaagaac cagggcgaat tgttccgaat caataatctg atcaattcta
     4201 atgtttttga ttgttgccgt aaggctggca aggagaaaat caagggtgtt atctatgttg
     4261 gtaccgtatg ctccttccct ctgaccagac agaatacttt gaatcctgag cctctccggg
     4321 aagaggagtt gttccctgct cttcctgagt ctgcttatgg ctggagcaaa ctgatgggcc
     4381 agttggagat ggggtatttg gaaaaagaaa ccggaattcc gtgctgtatc ctgatgcttc
     4441 ataatgttta tggcacacct actgattttg gcgaacgtag tcaggttatt cctgctctga
     4501 ttcgtaaagc aatcaataat cctgaagagc cttttaatgt gtggggtagc ggagagcagg
     4561 gacgtgcttt cattcatgtc aatgatatcg taaatgcatt ggtgctcgcc ctggataagg
     4621 gttggggtca tggtcatatt cagattggcc cttctcactg tacttccatc aaggaaattg
     4681 catataagat cattgaaatt tctggcaaag acatcaagcc tttctttgat acttctaagc
     4741 ctgaaggtga taaggctcgt tgcgcagatt attctaaggc gaaagagatt ttggattgga
     4801 agcctactgt ttctttggaa gagggtcttc gtgaatccta caattggatt gaaaagcaga
     4861 ttaagggtta ataagtatat aagctgttct tcctccgtgt aattagagga tgcggaggaa
     4921 gaacatatga gtaaggatgt actgagatgg aaaagaatat gtataaaaga aaacgtgatt
     4981 tcactgtgtg tggaatagta ggtgtttgtg ctactacaaa gaacgagttt gtaagggaag
     5041 ttttttccat gccaacatct ccggtaagtg cgactgttga tctcgtggga gtgccggcca
     5101 ttatttcggc aaaggaaaat cccaaaattg ctgagatgta tagccaggct acaatggctg
     5161 caatagatgg tatgcctatt gttaaaattg gacggcgcaa aggatttacc tgtgaacgat
     5221 gtgctgcacc agatattatg ggaatggttt ttgaagaagg cgttagacag ggaaaaacac
     5281 attatttcta tggtggaaaa gataacgaag tgttaaataa gcttcgtgca aacctagaaa
     5341 gagattaccc gggaatcaaa attgttggaa tgtattcccc accatttcgt ccgttaaatg
     5401 aagaagagga tagcaagatt tgcgaggaaa tcaacaactt aaatccagat tttctgtggg
     5461 ttggtattgg tgcgcctaag caagaaatgt ggatgtggga acaccagaag aagatccata
     5521 acacagtaat gttcggtgta ggcgccggct ttaacttttt tgcgggcact ttggaaaaag
     5581 ctcctgaatg gatggaaaaa gctagtctag agtggttatt tagactggta aaggaacctc
     5641 gtagactatg gaggcggtat atcttgggag gtataaagta tatctactat actattgaat
     5701 ctaagtaggg gggcaataaa aatagatgaa aatacttata gtagttacaa tgacgaataa
     5761 ctttggtaag aaggggtcct ataattctca ggaaatagga ttagctcgag ggttagctga
     5821 attaggccac gaagttattg tatataaaag tgtaaaaagt gatgaggaag ttggaacaga
     5881 gcaattaagc aatacggcga tgattcacta tatcaagtgt cgttatgtag gatcaaatgg
     5941 attcttatca acaaagaatt ttgataagaa tgcggatgtt ctaatacaat ttgcggacct
     6001 tcaactatca gtacctcatg tatactcatg gactgtaaaa aatggaataa aatatatacc
     6061 ttataatggg ataacgcgaa gtcaaagtgg caataaatat ataaagacaa ttattgaaat
     6121 attggcgcat cgaaattata aaatttataa gaaaaatggg tgtgttgcca aaaatgatcg
     6181 agtttctgaa aaattagtac aattaggagt aaataaagtg aaacttgctc ctgtttgtgt
     6241 ggattttgaa cagttacata agaattacga agcaatagat tttactactc tcagagaaaa
     6301 atggaatatt cctcgcgatg ttcgtgttat tttatatgta ggacggtttg aaaaggataa
     6361 aagaccgtta gatatgatta gtttatttaa taaacttgat gagcataatt atttgctgat
     6421 ggtaggaact ggtgctttac tcgagcaaac gaagcagcag ataaatttac ttggactgca
     6481 gaatagagta aaaataattg agcgtattcc taacaaagat atttgggagt tatattgctt
     6541 atcggatgta tttgtaaatc taaactatac tgaaatttgg ggtatggttt tgctagaggc
     6601 aatgtactat cgcctcggaa ttattgctgt taatgcacct ggcccacaga atattatttt
     6661 ggataataga tatggaatgt tggctaaatc tgacgatgaa attttgacat tcttaaaaga
     6721 cggacgattt accaacgata tcatagatgc agcatatcag cgtgtaacta cagaatttag
     6781 atggtcaaag tcgggagaaa ttatgcttag catgttggaa ggtgataaat gatatggaag
     6841 taacgaaatt aatggcattg aggaatcgat atgctttaaa catagttgat aattgcacga
     6901 gaaaaatagc caaaattctt ggttgttgta ttggtaaagg tgcacagata gggaactctg
     6961 ttgaatttgt tcataattct gtaggcactg tgattcattc tgacacaatc cttgaagatg
     7021 gtgttaaagt ttatcagaat gtgacgtgtg gttgagcaga tagtaatgaa cgtatcatca
     7081 ttcgagtaaa aaagaacgct gttttatgtg tgggtgctaa aattctttgt aaatctaaca
     7141 cagaattatg cgttggggaa ggtgctgtag ttgctgcaaa tacagtggta ttgaggtcta
     7201 ttccacccta cgaagtgtgg ggggtacctg ctaaaaagat ggggagataa aacatgaatg
     7261 catataaatt atacaaagta gaacgatggt tttacttaca caaaatggag ttttttgcca
     7321 agattgtaaa ggcactaatt tatgtacttc ataatagctc aatttcatat caaacagaaa
     7381 ttggagataa ttgtaagttc ttatacggtg gcataggttg tgtaattgga aaagatacaa
     7441 aaataggtaa tcacgttata atcggaacga atgtactgac tggtggtaga tccaataagc
     7501 ctggtatgcc tacaattgga aacaatgtat atatcgctac tggcgcaaaa attttaggta
     7561 atatcacaat aggggacaat gtaatagtag gggctaatgc tgtggtcatt aatgatgttg
     7621 aacctaattg ttcggtagga ggcgttccgg ctagagtatt gaaaaaagat attgatgtaa
     7681 atgattattg tagtttgaat cgttactaaa aggagagatt gtatgttgta ttttgttatt
     7741 cctttaatga gtgcgaaggc agcaaacaat tgggagatgg ttagtacctt atttaaccga
     7801 acattatggt cgtgttataa tcagactgat cctgatttca aaatcattgt tgcttgccat
     7861 gaaattccta aactcacgca tgaatatgat gacagagttg aatttattca agttagcgaa
     7921 aaggatgccc ctattcccac aaatcaagac gaaaaaatga ttgataaggg ttataaaaca
     7981 catgctctcg caatgcgttt gcgagagttg ggcggcggtt tcgctatgat ggttgacgca
     8041 gatgatcttg taagttctca tttggcggaa tatgtgaatt cccatcccca ggaaaatgga
     8101 ttttatgtca aaacgggata cgtgtacttt gttggagacg attatatgaa ggttctacca
     8161 aagttctcat caggaagtgc gtgtatagta aaatatgcag tggaagacct gcctgaggca
     8221 tatccggaag ttatgacagc aaattgtgat gataatccat ggatcattag aaaacgacat
     8281 gggggcgttg ttccagcatg tgaagagcaa gggcgtccat taaaacctct tccattcaaa
     8341 gcagcagtat atgtattagg aacaggggac aatcactcgc tttatggtaa aacgacaaaa
     8401 tatcagacga agatgcgaga gatactggaa atgtttatag caaaacgtaa aattaaagga
     8461 aaactgaaag cagaattttc tgttgattgg ttataaggtg agtattatga aggtattgat
     8521 gtgtgaaagc caaatgccag agaattggta ttcgtttgat ctttctaaag aacttaatca
     8581 atatgttgat ttaacattgc tgtgtaagcg tgatgttcct cctttagatc ctaaaattaa
     8641 gagcaaacat attttgtatc ataaaagtac aaataaagtg ttttcggcac ttgtttttct
     8701 gaaaagcttg ttcttaattt ggaaggaaat aagatttggt gattatgata ttgttcattt
     8761 gcaattgatg aaaagtctca aatatgaagg cggattgtac gttttatgta agagacacta
     8821 taaaaaatta attatgacag ttcataatgt gttaccacat gaggttcgtg aaaaggatcg
     8881 ccaagagcac tttaatatat attcaaattg tgatggtttc attgttcaca atcgagtaac
     8941 agaacagcat ttatatgctg aattcccaga attaaatgca aagtcatatg tttgtccaca
     9001 tgctatctat ggagatgtta aacatatatc tagagcgtat gataaagata agtttgaatt
     9061 tatcttgttt ggacaaattc gtccatataa gggaattgat gtactgctta atgcaatcac
     9121 agaacttcca atggatgtaa gaaagaaagt tcattttacc atagcaggtc aacaacatcc
     9181 taaacaggat agtactgact ataaaaaaat gattgaagat ttaaaggttt cagattgtgt
     9241 tctatataag ccagagagaa tttctgatga agaaatggac agtatgttca gaaatgcaga
     9301 tgccgcaata tttccatata aagaaatcta tggaagtgga gcgcttctta tggcatacac
     9361 ttattgctta ccaactattg ttagtaatgt acctgctttt atggaagaaa ccgaggaagg
     9421 aaagataggg ttacttttcg aaaatttgaa ttctaagagc ctagcagaca caattgtaaa
     9481 gtatgtgaac cttagcccag ctgagaaaag caaaatagag gatgatatat atgagcttgt
     9541 aaaccgcaag tatacctggg cggcatctgc aaaactcaca tacgaggcat acaaacaaat
     9601 tttgaatgag tagtaaaaca attagaaagg aataataatt aacatgcaga ttagtaaatg
     9661 gaaaatcata cagtttctaa tggtctttct aatgattatc tcatcaagca tattgaagcc
     9721 tttgggcggc tggttgggag aaacgatgaa cgttagactt gtgacagtca ttctagcaat
     9781 agtatgtttg ttaatagcct cattaccagc atttagaaaa aatagatatt taaatttctg
     9841 cactttactg atttgtgttg ttataatagc agcttacatc tactcagata agacaatatg
     9901 ggttaaatct agggaaagta tgagtttata ctatgtatta ttggcgtatc cattgttctg
     9961 tactttaatt aaccaagcgt ggaatttgga ttcaatgcta aatacgatat gtggtgtaac
    10021 atttctttca tacattctta gaacatctat ttcacttatt cagaaatttt caggagtact
    10081 tctctatgaa aacatatatc gtgagtgtgc cccggaaaat tggtatcgtg gaggcttttt
    10141 aagaattaat ccaccatgtt ttacaattat aataataccg attgctttgt atatgattga
    10201 gcgacaagtt gtagctagaa gaaaagtaaa atattattta ctgattgcta gcgcactggc
    10261 atattctgct gttattcatg gttctagatc tgtccttgtc tatcaaatta ttgtacttgg
    10321 ttgctatatc ctttttaaga aaggttcttc gaaaagaaaa attgcaatgt gggttttagc
    10381 tgggttgctt gtagtcatat ttattaattc gaccatgttc tcaacatttg ttgaaacttt
    10441 cacaaacagt acaggtgaat atgcaggatc tgctgaatct cgatttgctt cagtttggtt
    10501 ttttgttcct aaatttttgc aatcgccttt gttcggtact ggtattctta ctggtgagga
    10561 atttacgttt atattaccag gaggagttag aggacaccta tctgatattg gatttttcta
    10621 tacaattact caatatggtg taggtatact tatatttgat attctgttta ttataaaagg
    10681 gttcaaaaca gcattacttg catcaaaagt gggtatgact ggctatcaat atttagcatt
    10741 tggaattaca ttaagtgttt tgctaacagg gattaatata gactggtttt atccgatatt
    10801 tactccagct attcctgtat gcttagcggt tatagaatac atctatcaag aatgtagaag
    10861 tgtggctaac atagaatagg aaatattaat aggagtgcat ctcatgaata agaaagagat
    10921 tttatataac atattgaaga agtacgaaaa tttaccatat gtgtttagga acatgtttgg
    10981 tcataatata agtaaactcg tatcttggaa attgactgct gattgtgcaa cagtagaaga
    11041 aaattctgaa gtttatgcag cgtttcaatt gcgctcaaag gacatgtctg atgttccagt
    11101 gatgggttta gttaattgtt gcaaagatgt agcaattgta atgcaggggc cactatgttt
    11161 aaaggatgat ttctcttata atactctggc ctactataag aaatgttatc caaatgcttt
    11221 aattattctt tcgacatgga aggacgaaga tattaaggca ttgaaaagga tagaggatct
    11281 tggagttata gttatttgta gtgagaaacc tgaaaagcct ggacacttaa atattaatta
    11341 tcaagttgga aacactttgc ctggaatagt cagagcaaag cagcttggtg caaagtatgt
    11401 ttgtaaaact agaacagatc aaagaatata ccatccaaat gcaatggcgt ttttctgcag
    11461 tttacttgaa cagtatcctg ttaataacga agatgattgg aaattaatac aaaatcagcg
    11521 attactgtta ctatcaatgc cgtatggaga catgtttttc ccgtattgtt tgtcagactt
    11581 cctgtatttt ggtgatgttg atgatatgat aaacctcttc tcgattccta gtgatgtgag
    11641 agaaaaggga gctgtgtcta gaggagtctc aagaagggag atatctgaaa ataatttagc
    11701 tccagaagtt cagcttcttc gaagctatat ttcacgaatg gggggcaatg aggagtgctc
    11761 tattagggca tattgggagt ttgtaaaaaa ccatgttatt actattaaca aaaatcaaat
    11821 tgatttgtac tggcataaat atgtaggtcg ttattcaaat aatactattt atggtacgta
    11881 ttatattgat gatagccaag atgcacttca ctgctataat tttgatttta taaattggtt
    11941 gaatttgtac actggaaaat acgaatacaa agcaaaatat gaaaaatata tggactttgt
    12001 ttgggaagag taattaactt ttatagtaaa aagtacttta gggaagggca tattatgaaa
    12061 acagtaatta tggcgggcgg acgaggaagc cgaatttcgg aattgtttcc ggatattcct
    12121 aagccaatga ttcctataga tggcattcca gttttagaac gagaattgtg ttcgcttaga
    12181 gatcaaggct ttaatgatat tattttgact gtttcctatt tgcatgaaaa aatagaggag
    12241 catttcggag atggttcaaa attaggaatt aaaatcaaat attttattga aaaaactcca
    12301 cttggcaatg caggtgcatt gtattttttg ttggatgaac tgggtgatga accgttttta
    12361 cttttgaatg ctgatgcagt atttgatgtt gatttcaggc agatgattag atttcatgtt
    12421 gagaataacg cagatgttac tttgtttacg catcctaact cacatccgta tgatagtgga
    12481 ttaattatat ccgatgacaa taagaaagtg cttcaatggt tagcgaagga agatcaaaga
    12541 cctgaatatt ataaaaaccg agtaaatgca ggtcttcatg ttataaatcc gaaggtgttg
    12601 agagaaaatc cattaatgga aattgtaaat aaatcaatag ttgaaggtgg ggggctacca
    12661 tacaaaattg atttagatag aatgattctg aaaccattgg ctaacacagg gcgtatgtat
    12721 tgctacgatt ctccagagta tgttaaagat atgggaacac cggaccgata tagagctgta
    12781 attaaggact ttcaaaaggg aattattgtt ggtaaaaatc tatccaatcc acaaaaagca
    12841 atctttttag atagagatgg aacgattaat aaatacgttg gttttttgag aaatgcagaa
    12901 gaatttgagt taattgatgg agtatcggag gcaattaaga ttattaattc tctaggtttt
    12961 ctgtgtattg ttataacaaa tcagcctgta attgcaagag gtgaagttac tgttgatgag
    13021 cttaatagta ttcacaataa gcttgaaaca atgctagggc atgctggtgg atttttaaac
    13081 ggtatttatt actgtccgca ccatccccat aaaggatatg caggagaggt tatagaatta
    13141 aaaattgatt gcaattgtcg caagccaaaa cctggactgt tattagaagc atcaaaggat
    13201 tttaacataa acctaagtca atcatttatg gttggagata gtgaaagtga tgtaattgct
    13261 ggaaaaaatg ctggttgtaa gacagtactg attacagaag acatcacctt gcctgctaat
    13321 tatggccaag actatagtgt ctgttcattg ttagaatttg ttaaaaatca tttatgcact
    13381 atgcaataat tggtttagtg aaacttgtaa aaggagtaat tagatgaagg tattaattcc
    13441 agcagcaaaa gttgttgaaa aagaattgca gagtatagga atgttaccag caattatcta
    13501 tccaattaat aaaaaaattg tctttgatta tttgtataat cagtataatg caatctgcga
    13561 ttcattggat attatctgtt atgaaaaagc ggataaggtt catagaagac tgaaaaacta
    13621 tcatgattcc agaattcata tatgtgacct tcctgaatta ggggatctag ggcaaaccgt
    13681 gtattttggg ttatcagatg aagatgagcc tgttctaatt aacttcgcag atacaattgt
    13741 tatggagagt atgtctgata tagaaatgga tacattctat tattctgaag attttgtgtc
    13801 gagtacttgg acatacttta aaattaatga aggaataatt actgatatcc acgataaaga
    13861 ggaagcttac gtatccgata aacgtgaaaa attatttatt ggcgtattta tgttttcttc
    13921 gtcaaaactt ctgaagagtt gcttaagaga ggcagttgag agtcggaaac aggggggaat
    13981 aaactcattt tatcgggctt tacagctata cagcgaacgt tgtccgctaa aggccgtaag
    14041 aacagaaaag tggtttgata ttggacacat cgataaatat tacaatacaa agttggaagt
    14101 taaagcacga gagtttaatc atatctctat agataaagat cgagggatat tgaagaaaac
    14161 tagcgatgac aaggataaat ttattggaga aataaagtgg tatttaaaac ttcctgctga
    14221 tattgaatat gtaagaccta gaatttttga ttattctaca tcttacgtag ctccctatgt
    14281 tgatatggaa tattatgcat atcatacggt tcatgagtta tacttatatg gtgatttaaa
    14341 atataaccag tggcatgatc tgtttagccg tataaagttt atctgtagcg attttaagcg
    14401 atacacctta aaagatgaaa agattagaga gtcattggaa gatatgtatt tgcgaaaaac
    14461 catgcagaga tttcaaaaat tgtctgaaga taaaagattt tcagcttttt ttgagaaaca
    14521 aattcaaatt aatggtcgta agtttatttc tttaaccggt gtaattgaac ttctaaagaa
    14581 tcttatccca agtatgctct atgatgtaaa tgaattttgt attatacatg gagatttgtg
    14641 ttttgctaat atgatggtag attccaattg ttcctttgtt aaggttattg atcctagggg
    14701 aaaatttgga aaatatgata tttatgggga cagcagatat gaattagcaa aactatttca
    14761 tagtttagat ggtaaatatg attatatcat caaggatcta tttactgtag aatatgatac
    14821 cgagtgtgca gtgatagatt atacaattaa tacgcgtgta agagattacg atttatttga
    14881 attgtttaag tcggtctttt cggacgagat taaggacgat tttgataaga ttgtattgat
    14941 agaagcgttg ctatttctta gtatgattcc attacacggt gaaagttttg atcaccaaat
    15001 agtgatgctt gctacaggta tggaacttct tgcaagagta agtaataaag ttatactcta
    15061 atataaattg tgccttaatg aataacggtt acaatactgt tttagcaatg taggcattaa
    15121 ggcacgatta tattaattaa tacaatagaa gggaagagaa aaatgtatag tgaatattct
    15181 tttgtgtttg atatcgatgg cactttgtgt ccgataaaga agaaggatga gcgatatgag
    15241 gatttggtgc cgtatgagaa tatggttgaa aaactgagat tctataagga gaatggtgct
    15301 aaaattacat tgttcacctc acgcaatgtt aattcctata aaggaaattt gggaatgata
    15361 aatgctaata ctgcaaaagt gctcttggcg tggttggata aatgggacat tccgtatgat
    15421 gaaatcctgt atggtaagcc ttggccgggg cataaaggct tctatgtaga tgatcgaacg
    15481 gttagacctg atgagttttt gagatgctct gtagatgaat taaatgaaat ctgcaacagt
    15541 tcaaaaaaaa gattctaaat aaggaggtta gttctatgga aaaaaagatg acagttatca
    15601 ttacgatggc aggtttggga agtcgttttc gaaaaattgg ttatgcggtt cctaaatata
    15661 tgattaatgc caaaggaaga actttatttg attgggccat ggatagtttg gttggataca
    15721 ataaaaatgt tgataagtat atttttgttg ttcgtcagga agacaacgct tcagctttta
    15781 taaaggagca ttgtgcttac tttggtattg aaaacgtaga agtgattgaa atagactata
    15841 tgacagatgg tcaagcaact acatgtatgt tggctattcc ttattgcaat caagacaatg
    15901 agattatggt ttacaatatt gatacatacg tggaatcaaa tgagatgaaa tacgaagata
    15961 tttgtggaga tggacatatt ccatgctttc atgctgatgg cgatcattgg agttttgcaa
    16021 agcttaacga tcgaaatgaa gtaatagaag tccgcgaaaa agttcgcatt tctgataatt
    16081 gtactcttgg tgcgtattat ttctcatcgg caaagctgta taaagcttta tatgaagagt
    16141 attatgcaga ctcctctaat atggagaaga atgaaaaata tatagcacca ttatataact
    16201 ttatgataaa aaaagggtta aaggtgacta ttagcatagt agatgcgaaa aaggttcatg
    16261 tgcttggtac tccagaagag ctcaatgtgt ttttgaatca agaataaata gtgtttagga
    16321 gaaaatagca gtgaccagta ataatctaat tgtaaaaaca ttaagcagat ataagaatac
    16381 tccacttcct gttaaggctg cattatggtt tatggtatgt ggttttttac agagaggggt
    16441 ttcaatgatt acaaccccaa tttttactag agttttgtct acagaacaat acggtttata
    16501 cagcacttat ctatcgtggg aatccttatt gctcatgatt acatcgctta gtttgttcaa
    16561 agccatgatg aatctttatg taaaatatga taatcaagaa catattcttt cgacagtatc
    16621 cggattgact ttagtactga ctattttttg gtttgttatt gctattttta ttaaggatat
    16681 attggcagaa attttaggat tgacaccagt tctagttgtc tgtatgttct catattttat
    16741 atttcaggca gaaatacaat gttggtcttt acacaaacga tatatatatg attataagac
    16801 tttattagta gctacccttg cagttactgt tggctcatct gtagttggct gtttagcggt
    16861 tctgtttata tctcctaccg ctgaggcgag agccttaatg aatgtcctag tgactggtgt
    16921 tgttggcgtg gctgtgtata tatcggtatt tagaagatgt aaggatttta aggataatga
    16981 agtgtggaat ttcgcagtag tattttgtgt atcgttgatg ccacactatt tatctgaatt
    17041 tattctgaat ggttctgata aaattatgat aaattactta tgtggttcgt cagatgttgc
    17101 tatttacagc gtggcatatt ctattggtgc tctaattaac cttgttacaa attctgtcaa
    17161 ttccactttt gcaccttatc agtatcaaaa aatcaagtca ggagaatatg atttactatc
    17221 aaaaagagct aatgaagttc ttggtttggt agcggctatg ctttttgcat taatgctatt
    17281 tagtaaagaa attgtgttga tatttggtgg gcagaaatat atcgacagtg ttaatttaat
    17341 tgtacctata tgtatcggta tttatttcaa ctatatgttt cagctgtttg ctagagtaca
    17401 ggaatacttt gaaaagaaac tcactgtcgt tattccatcg gttttgtgtg caattatgaa
    17461 tattgtacta aattatgttt ttataaagtt atatgggtat caggcggtag catacacaac
    17521 tgtattctca tatatgtttt tctgcgtcat ccattatatt ttttatagaa aagtttgcaa
    17581 agaactattg gatggaaaaa acatatatga tgtcagagga atacttttga tttctgcaat
    17641 cacgatagct ggtggcatcc taatttcaat aattaataaa tttgatttca taaaatattt
    17701 aattatagca attgcttgtg tcgctgcaat tatatttaga aaacgattgc tacttttagt
    17761 aaggagaatt atgagcaaat aaacatttgc tcatagagag atagagaatg atagatatta
    17821 tttctgctat aatggtaaat gtattaacag cattatatca gccgttctta tttgccgtat
    17881 taacagcgta tctgtttatg tactggtatc tatttatagt tgataactct cgagatccta
    17941 aaaacttaaa agattctgta ttaatctggt tgagatattt taaagcatct agtaaatttc
    18001 gcagactctt ttttcttgcg ttttatttta ctttgatatt atttaaaacc ttgcttaatc
    18061 gaaatatgtg gatgaacccg ctttcaaacg ttattggaca gtggtcatta tatattatag
    18121 ataatgctac aggagagagc agattgacaa cagaatgcgt ggagaacacg atgctgttta
    18181 tccctttttg catgttattg ttatggtggc gtgacattaa atgtgatatt atacgcacca
    18241 tctatgttgg gattaagtat gtgtttttat tctcattaag tatagaattt acacaactgt
    18301 tttttaggct tggtacattt caactatcgg atttgtttta taacacccta gggggcttaa
    18361 ttggtgcttt gctatattgg ctattctacc gattaaataa gtatatagac ttaaaataga
    18421 actgctttga attttaatat ggttaatatt tttctgcaga caggattgtg taatctcatc
    18481 aagaatactt tacatgccat ctatttttgc taaaacattg ttgttatatc ttcaagaggt
    18541 agggaatttt attgctttaa atgaggtcga gcgaattaaa aaattggtaa aatatataac
    18601 atggtggtag ttatggtaaa agtgtttgat ataattaagt tttcggattt cagaggatat
    18661 actgctgttg cattggataa tgagactctt ttctcgattg gtattgagtt tcacattaag
    18721 caaataaatc aaggatttag tcataataaa tatactatgc gagggctaca ttatcagagt
    18781 ggagagcatg ctcaaggaaa gatagtgatg tgcctacatg gtgaaattat gaatatcgca
    18841 cttgatattc gacaaggatc ttccacttat ggatgtgtgt atgtcaacac attgagtgaa
    18901 agcaatggta aatgtgcgta tatcccacgt ggatatgctc atggctatct tactatggag
    18961 gacaatacgc tagttcagtg gtgcgtagat aatgatttca attctaatgc tgccaaatgt
    19021 ataagttatg aaagcgtaga tattgaatgg ccggtcagtc ggcagtgttt cgttataagt
    19081 gagaaagaca aaaatgggga aagattaagc ttttgattaa aatgagctgt ggagaaaaga
    19141 aggatactgg aaaataagaa gaacaataac cgagcaaatg ctcggttatt tctatgagga
    19201 atacataaaa ctaaatttag atatagatat tt
//
LOCUS       DACEPW010000040        18576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEPW010000040 DACEPW010000000
VERSION     DACEPW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.041"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(52..279)
                     /locus_tag="IW819_001097"
     CDS             complement(52..279)
                     /locus_tag="IW819_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244216.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(445..2415)
                     /locus_tag="IW819_001098"
     CDS             complement(445..2415)
                     /locus_tag="IW819_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244217.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2430..3296)
                     /locus_tag="IW819_001099"
     CDS             complement(2430..3296)
                     /locus_tag="IW819_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244218.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3286..3519)
                     /locus_tag="IW819_001100"
     CDS             complement(3286..3519)
                     /locus_tag="IW819_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1244219.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3531..5693)
                     /locus_tag="IW819_001101"
     CDS             complement(3531..5693)
                     /locus_tag="IW819_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244220.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYQKEEKEAKAKK
                     AKVKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEEVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLLIPDDPNMFESEVEEATDFYSAVEQLEVATN
                     GLERLAETFNSSEEVEFEALAEDIPDEKEALFADDDKPTEAENQEVSIEETQTQIQTE
                     TYQQSKIDEKPESQSDKENYVYEVEKIVSESDVPKSLDADFLEKNFEQIIADKFQPVV
                     LDEINDPVDLNEEMLSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGK
                     QMLADELLRIEQATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIENSLTDVQEKTLEIVEKTKSENEAEFHDTKEKITDVVVK
                     YFANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYRSNSEKLAEQS
                     EALREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIIAVTAMACGTIL
                     GSSYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVIS
                     IDGKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5693..5935)
                     /locus_tag="IW819_001102"
     CDS             complement(5693..5935)
                     /locus_tag="IW819_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244221.1"
                     /translation="MVFDREKMLAHANEVLMSGLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTIDRKKGVLQ"
     gene            complement(6045..6383)
                     /locus_tag="IW819_001103"
     CDS             complement(6045..6383)
                     /locus_tag="IW819_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244222.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNEQLG"
     gene            complement(6782..10423)
                     /locus_tag="IW819_001104"
     CDS             complement(6782..10423)
                     /locus_tag="IW819_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244223.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLIDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDENGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGNGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLSKETDKEQSDVKKKFIPTPNINQTDKYETNNGLGMSKDNQSIREP
                     ELTNDNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKSP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10426..12375)
                     /locus_tag="IW819_001105"
     CDS             complement(10426..12375)
                     /locus_tag="IW819_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1244224.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFG
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPKKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12417..13475)
                     /locus_tag="IW819_001106"
     CDS             complement(12417..13475)
                     /locus_tag="IW819_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ1244225.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13594..14049)
                     /locus_tag="IW819_001107"
     CDS             complement(13594..14049)
                     /locus_tag="IW819_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244226.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(14633..15436)
                     /locus_tag="IW819_001108"
     CDS             complement(14633..15436)
                     /locus_tag="IW819_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1244227.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            16394..17617
                     /locus_tag="IW819_001109"
     CDS             16394..17617
                     /locus_tag="IW819_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ1244228.1"
                     /translation="MESYQFQIFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKKFD
                     LTVTLSNELVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            17652..18419
                     /locus_tag="IW819_001110"
     CDS             17652..18419
                     /locus_tag="IW819_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1244229.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
BASE COUNT         5588 a         3578 c         2660 g         6750 t
ORIGIN      
        1 ggctgagatc acctttcaat ctaaatacac ggttcttttc tggtaactta ttcatgcttc
       61 aaccacctca tactgttctc tcataccttt gacttcatta aaaaaaactc tcgctttttc
      121 ttcatcagta aagtttttat acaactgatt acaataatga ggtttttgaa gtttctgaat
      181 acctatacta acttcaaatc cttcttcatg ttgaattagg attacttctg tgtctctacc
      241 tagaaatatt cgatctaaaa tttttatgtt tggtttcatt atttaacctc cactataatt
      301 atttcactca gtaaggtttc gctttatgaa atttctatag ttctcttttc acaaaacgta
      361 ccatgttctc taagactgtt tttagtctca aatatacatg tttttcttat cctttcctta
      421 tacactcaaa atcaaagata aaacttaaag aataaactct ccaataacaa aattattagt
      481 cagatccact ggtctcctga tctgaaattt attacttctt cctgttgtca aatactgtct
      541 caaatcaaca gaaagttttt gatcatcttc tccttttcta gcaggagcaa cttttttttc
      601 atacttcatt aactctccac tggtcatctt tccaatgata gtgtaattaa aatcactttc
      661 aaaactttgg taaatttttc catctgtatt gaatatatca gctgtttcaa tatcttcttt
      721 gtattcattg tcttctcctg aaccgattgt atcaaataga aaggcaagac gaattccatc
      781 ttcttcaagc attgttaatc tttttttaat tagcccaacg gtttcaatag ataacttatc
      841 gaagccgtga atcataacaa tatcatcttt ttttgcttgt gatgtgatga aatcaaaggt
      901 atttaaaaat tgagcttcca taacattggt ttctgctctt agatcaccta agttatagta
      961 aaactgtaat ttgtcacttt taccgatttc tttcagtgtc gttctttgat taaacatcct
     1021 agagaagctg tctaatgctg ttctcaatgt gtcttttaat acaataatac gatcgacttc
     1081 tttttcagta ccagattttt tggcttttgt ttcagcatta gtcaaatagc taataaaatc
     1141 cccaagaact ggcattgtat cactactgtt taccaaaagt tttgaacgtt cttcataatt
     1201 aatatcccaa agtttctgac ttatataaaa gttacctaat tcttttttca ataaaactct
     1261 agttgcagaa tctaatgttc tgcctgctaa caaataaaaa atctgagtta gtttatcgag
     1321 attccgtcta tataaatcac caatgtcctt ttgattacca aaaagctcca aagagttaag
     1381 accttcttca gccaaattaa ccttttctat tacactgttt aacgaaggtt tggcaacaaa
     1441 tgttctacca gttggatcac caaagtaatc aaatttattt aagacaatat gaaaagttcg
     1501 tttatgattt gccattgcat tattagcaat caattgcccc caaagtgatg atgctgagat
     1561 tttttcatca tagcctttaa ttgtagcgtt tttgtgtgat gccactaaaa ctttacttgt
     1621 aaaagaatca tccagtgcca taaaagctac accacttgcg tagctagtag acatagtgcc
     1681 gatagcccat ccattcgcat catctaaacc tttgcgaatt gcatgatcat ttcctgcaaa
     1741 atccgaactc atagacgtat actcatagac attcccgatt ggttcactta aaagtctatc
     1801 aaacagatca cgttgatctc ctccatatga cataagcttt aatccataca ttttttcatc
     1861 atacaaccgt tggagtttaa ttagctgttc gctgatttta ttaggagaat cggatactaa
     1921 taggatttct agcttccaat caataatttt atttgccatg ctgtctagta aagatgctgt
     1981 attcaaatcc ataatccgca taacagtttt gctaatttca gcatctgaat tttttccttc
     2041 taaattattt tcataactat tcgttgatcc cccaacattc tcgtgcatta tttcttgttg
     2101 atctttttgt gacactggct tatctgcttc aattagatag gcttttaccc cttctactct
     2161 cacttcagga atagctttaa ccatccaacc aaatccataa tttctattca ttccataagt
     2221 attcactaat ttcacaattg ttccccaccg cttacctgtc ctaatgtaag atggtgtaaa
     2281 ttgataacca actaaagatg ttaaatcact aacaaaggta ttattcacta cctttgaatt
     2341 tctagttttt tgttcttttt tagtttttct cttttttttg aaatttaata gttgtgatct
     2401 acccataatt tccatttaat ctctcacctc tatttcagtg cataaatatc agataatatt
     2461 tcttttgttt gtttagctga caactgagtg acactataat aaagtcctct actcgtatag
     2521 ttttctaaac gttccacacc ttcatccaat accatcttag attgagattt caagataaga
     2581 tattggatta ctgttgtttt cacaccatca atatattctg atacaaatcg ttcttgctga
     2641 tctattatgt caaagactgc tttgttactc cttgcattcc gtttttgtat tttcatattt
     2701 cgagtttgtc tttccgtatt ctgaggttga gatgaagtga ttttgatttc agctaaggaa
     2761 cgatctctgg tattttgata ttgtattgat ttcgattcct gagccataac ttcttctgga
     2821 aaagcggtta gtgatgtagc cccatctaga ttgaacatta atgctacact cccatctcga
     2881 tatacgattg ttccatcttg tttcactttt aaaatctctg tgcctcgaaa tggtttcttt
     2941 ttccccatag atactcttgc tgaattaact actgatttga acagtctaat agaaccagaa
     3001 atcaatgaac cactagatgg atcaataaac atatactggc taattagaat aagaaatcca
     3061 attatcatta ataacgttgg aacaaaaata aattttagtt taatcgtatc agaaactaag
     3121 taaacaattg atccaagaac aacgactgca acaactatag ctgatacaat taatcttttt
     3181 gctgtaacag aataggtgct attgacatgc ttttcaagcc attcaactct gacaattgtt
     3241 tcagaaggtt tttgatgtaa gttatgttta aatccatagt ttgagttatc tgccatcaga
     3301 aatacttcct ttcacgttca acttacggta aacacctaca gttatcacta ttgcaacaat
     3361 aagcaagcca attaaagata ttgacttgct cattgtctcg ttcgtttgtg gtagttcatt
     3421 tacctgatac acgagctgtg cttcctgtaa gtgctgagaa gaggtcatgt tttcttgatt
     3481 gttttgaatt actggataaa ctattttttc ttctcgcata atagccctcc ttagttatta
     3541 tcagataagt gagcttgaat tttgtactga tttccgtctg gagttttcac aactgctttt
     3601 tctccatcaa tagaaatcac ttcagcctta gttgttttcc catcaatact gatgacaaca
     3661 tctttcccaa ctttgaactc tgtagttggt gttgcttcat agctactatt tgttgaagca
     3721 gagttcgtat ttgtcccaat cgaattttcc actctcgaaa tagatgtttg agtttgagcc
     3781 tgtgcttgtt gattattatg actaataaag taggacgatc ccaaaatcgt tccgcaagcc
     3841 attgcagtaa cagcaatgat agtagccatc tttttacttg taaaatcacg ttttgaactt
     3901 gattgatttt ctgtagcaac tgctaatgat ttcgtttttt ctgccatttg agctagatca
     3961 cttcgggaac gagataagtc ttctcttaaa gcttcacttt gttctgccaa cttttcgcta
     4021 tttgatctat aaccctctgc taaatctttg acctctttca ttcgatcttt atattcttcg
     4081 atagtttttt gttgctctac aagttttgtt tctaattctt tcgtgttgtt agctacttgc
     4141 ttttcaactt cttctttgtt cgcaaaatat ttgacaacca catctgttat tttctctttt
     4201 gtatcatgga actcagcttc attttctgac tttgtttttt caacaatctc taacgttttt
     4261 tcttgcacat ctgttaatga gttttcaatt tctggtttca atgaattgat aatccgttcc
     4321 accacaactt cttttgcttc ttggttttgt ttcaatgcat cgtttttaat agattcaatt
     4381 gcttcttggt actgttgatc aagactttct tcagttgctt ttctatattc atcttcttgt
     4441 tgtttaactt ctggtaaatg ttcagcatca taccgttgct ctagtttctt tagttcagca
     4501 tccaaataag cctgtttcgc catatcataa tctttacgaa tgatactgga tttttccatc
     4561 actaaatcat caatcttaga tgcttctgct tcgtagctct ctttagcaac cgataatgta
     4621 tttgtaaact gattttgacc atttaatgag gttgcttgct ctatccgcaa aagctcatcg
     4681 gctaacattt gctttccttt gaggatcaat tgctccttcg tcttatttaa ttcactgttg
     4741 attttattat tagcgataga aatacgagca ttcatactct ctttagctaa aaacagcaat
     4801 ggtttcaact cagctatgga tagcatctcc tcgtttaaat caacaggatc attaatctcg
     4861 tctaaaacta caggttgaaa tttatcagcg atgatctgtt caaaattttt ttctaaaaaa
     4921 tcagcatcta agcttttcgg tacatcactt tcggatacga ttttttctac ttcataaaca
     4981 taattttctt tatcactctg ggattcgggt ttttcatcaa tttttgattg ttggtaggtt
     5041 tctgtttgta tttgagtttg tgtttcttct atgcttactt cttgattttc tgcttcggtt
     5101 ggtttgtcat catctgcaaa aagagcttct ttttcatctg gtatgtcttc tgctaaggct
     5161 tcaaattcaa cttcttcaga agagttgaag gtctcagcaa gtcgttctaa tccatttgta
     5221 gctacctcta attgctccac tgcgctatag aagtctgtag cttcttcaac ttctgattca
     5281 aacatattag gatcatccgg aataagtaat aattgaccac tactatccat aaaacggaca
     5341 cctgctaata gcttctcttt aaatttttct ggtagatcag ctacataatt caaacttgac
     5401 ttcaatagaa cgacatactt ttcggaatcg agaatagcat catcctcatc tataacttcc
     5461 tcactgttga gcttttgtac atcttcaatt accatataat ttttctttgg taaatctttg
     5521 gtcagcaacg catcgaattc tttccatacc tttaccttag ctttttttgc cttcgcttct
     5581 ttttcttctt tttggtacac ttcgagtaat tgtggcttca aagagagtgc aactacagga
     5641 actgtttctg ccacataatt aatagattcg gtaatatctt tgaattttgc cattattgta
     5701 aaactccttt ttttcggtca atagttcttt tctgtttgtc tagcatatat acatatgctt
     5761 tttcgaattt aataagcgtt tcaagtctgg ctttttcaat ctttttactc ccattccgat
     5821 aatcatagaa ctggttaaca ttcatattca ttatctttgc cagttcggta cctttcagac
     5881 cgctcattaa tacttcgttg gcgtgagcta acattttttc tctatcaaaa accatcttac
     5941 atttactttc ttccctcact tctaaactac gcttggagcg tagttttgtg gtaaaaagta
     6001 aattgaacaa atcggcatat tatccaaatt tgttcaattc acttttaacc taactgttca
     6061 ttttttggaa attttgaaac tagatagata ccaacaactg gaataagtgc tgatataata
     6121 ttagcttttt ggttcaaatc attacctgtt ttagtaccca ttgtgtaaag cataacggtt
     6181 gtaattacag ggataataaa gattgcaata atcgccccaa catattttaa tccatctccc
     6241 cattttgcat caccaaaaaa tttcattgca gtgaatcctg gtttcaacga aaaaacgaat
     6301 gccaagaaat acaaaacaac ttgtactaaa gttttcatat caggcgtcat tccgctaacc
     6361 aaatttttaa tagaatcgaa catataaaaa cctccttttt aatattttta ataagttaat
     6421 tatatcatcg acaaatctta tttgttcact tgtcaatgat ctaactaaga aaaagacgtc
     6481 tcgatattta catctttatg ttatcataat aacataaatt tttgatgatt aaaagagatt
     6541 ttccattaaa ttatttcact ttttttattg gctaaaaatg ctggtttatc aagtttttgt
     6601 ttagataata attctgttct ttaccagcat aagccctttt taattaaaca actttgaaca
     6661 gtaaatattt aattcattta tagataattt taaaaatttt tttgcataaa tagaaatttt
     6721 tcttgacatc ttcagttaaa aataaaagta aaaagaagga gcaaatcaat gctccttctt
     6781 tttattcgtc ttcagactta ttttcagttg atttctgatt accgtatttt acaatatagt
     6841 ccttgactga catggtagat ttcttcacta ctttaccagt cttggatgct cctgtagcag
     6901 tagccttggc aaccttagcc gttcctttac ctattttaat agcttcgttt ttagcttcat
     6961 aggttatccc tgctagacta ccttttggag attttgctaa agctttatct gctgaggtgt
     7021 aaacattttt accagttttc gatagatatt caacggctga tttggctgtt gatggtttct
     7081 tggttgattg attagatttc cctgaattat ctgaagtaga tttagaatta tttgttctat
     7141 tattatcatt tgtcagttca ggttctctaa ttgattgatt gtctttcgac attcctaatc
     7201 cattatttgt ttcatattta tctgtttgat taatattagg agtaggaatg aactttttct
     7261 tcacatctga ttgttcttta tccgtttctt tcgatagcga ttctctgtct ttaagtgctg
     7321 tgtcattccg aatagcacta ttatcaaagt tactgttaag ctctcttgac tgactttcac
     7381 cagatgctcc atttcctaat tcccgatgca ctctgtctga cacatcctca ccactcattt
     7441 catttcttag ttcttgatga gtttgatcta atgtaatctc gtcatcttca ttctcaggaa
     7501 ttgcaacagc aattggcgta tgccgttgtt gttcgagttc tctatttcga tctgccacaa
     7561 gcgcatctct cgtctcaatg atattcccat tctcatcgac taaatcgcca ttcacattat
     7621 atccatcgct gtgtttacca agtttattac caaactcatc aacaagattt ccatcttcat
     7681 caactaaacg ccctttttcg tcaactaaac gtccgttact atcctgacca aaatcaccat
     7741 ttagcaacct gcgtccattt tcgtcaacaa gatttccatc ttcatcaact aagcgacctt
     7801 tttcatcaat taaacgtcct tcgctatcca taccaatatt accatcttca ctatcttcca
     7861 atggtttacc attagtatct ataaggtttc catcttcatc gatcaaacgc cctttttcgt
     7921 caataaagtt gcctttagca tctttaccgt atttactatc ttttaatatt tcaccatttt
     7981 catcgacaaa gtttccgttt tcatcaacca tacgtcctaa atgatcaata tattgttggg
     8041 agttattacg tagttgatct tgacgaacta aatctttgaa agaggtatta ttgattgttg
     8101 aaccaactcc gccaccgttg aatgacattc cttctggtgt ttcaggaatt actttatcag
     8161 caatggactt agcatcagca ttgctatctc ccatattgtt caaacgttca gccatttttt
     8221 gcgctccaac tgctccaaga cctcctgcaa ccatatcttt tactgagctg ttgccaccat
     8281 cataataggg atttttagct ccttggcttt tcttgattgc tttagaaacg ccgtccacaa
     8341 ctcgcacagc atcatttcta ttaccaaata ataacttagc acgttcatca aacccaaacg
     8401 ctacttcaag tggtaagcca ataagcatat taatcaaatt atttactttt tcgccttttt
     8461 taaaaactaa aagatatcct acacctaaaa tcatagcaga tcgaacaata tttgctacgc
     8521 catcgccaac taatgtatta ggaatgaatc ccacagcagt atcaccaaat gcacgaataa
     8581 aagtaatcag aaagataggt aattgtagtc cgatcagaga tgttgtaaca acacctaatg
     8641 aaattgccat cacacctgct cctgatgcaa gtgatcctgt tagaactact tcccaaatcc
     8701 gaataaattc acgtttaaat gctttcacga tattcacatt gaacatcgcc caagcacatg
     8761 ctaagtaaag aactgctaaa acgattgatt cagcgttcat cgctagccac gagccaaact
     8821 tacccatcgc tccatgacca actaaggatg ctgtacgcca ttctgttttt actgttgaca
     8881 tattcttagc actgccttga attcctgaag gagcgatatt atacgcataa aacttagctc
     8941 ctgatgtatc aagtgccgat tggagcaata aagctaccga ctgtgtgcta aaagttgcat
     9001 tgttagcaac gccccaaggt tgagtagcat caaagttatg cgctgttcct tttccatcag
     9061 gcttgcgatt gtcgtctgtc acattttgat ttgctgacca aatatatcca tttaaattgg
     9121 ttggtttctt aattgcactt ccttcttttc catcccctaa attatagcct gtccaaacag
     9181 caggcgctct tcccatataa gcatcatttt cggcgacatt aacatcaccg gcataagtat
     9241 taatattgaa agtatcattc gatgaccatt cttttaatag agccataccg actgaattag
     9301 aatctagatc attattttgt ctggcataac tgctagtatt gatcgctctt atcattgcag
     9361 attttttaga tggttcataa tctgtatcaa cgaacttgat ttcggatgta ggctttccgt
     9421 caggtacatc accaccagta tcagatgatg gagcaagatt ttgagtagaa gcccaaagtc
     9481 ttgtgttaat gatgtagtca gaaacaacat ttgttggtat atctccatct ttttttgctg
     9541 actggatgcc gttgatataa actcccaata gcgatcctgc cactggtaag aatataaaca
     9601 atacaaaaat acgaacaatc catttttcaa gtactggtga taatcccttc gagttaacac
     9661 catctttcga caataatttc attccttgat aacctagtat tgctgtgaaa aagagcatag
     9721 ataagcttgc tatcgcttta aataattcat tatttaatcc caaattgtta agcaagctag
     9781 ttaatgcttt agataccgga ttagatacat ccgatccacc ttctccccac cccataagag
     9841 aaaatatatt catactcatg aaaaattttc caaggagttc atttatggat tcagcaatat
     9901 tagaaaggat taaactaatt gatccgataa aactaccaat cgtcctacca atacttccta
     9961 gaatggatga tgcttgactt gcaatagcat ttcctgagac tgtttcaata tatccgtagt
    10021 aactataagt tgctaaagta gatgctagtt gcgtacctgc attaccccct gcaatcgaat
    10081 taccttgttt agaaatgtca gaataaggga aatcggcact gataccacct tgtcctaaca
    10141 aattcatttg tccagtttta gcggcactat cattgacttc tttatctccc attaggatac
    10201 gagcatatag tgtccacatt ccttgttctg ccggtttact ttccgcataa gcaacgttct
    10261 tatttataac tgtttcttgt ttgctatctt gattgtcagg agttggatca ttcattccaa
    10321 aaggagcgcc taaagtaact gctaatactc ctccaataac tagacctctt acgacccatc
    10381 gttttattct aaggttttgt agtaaatatt tcttgaattt catagctacg cccctccaat
    10441 cgaaaataca atcttgtttt ctttactttc agcaacggaa ggctttgcaa taatttcagc
    10501 attagaatca ccttttcctt caaacgctcc ggcttcactc aacttaacat cgaatatttt
    10561 tgcgtttgga taagtaatta ttgcttcagt aatcgcttct tcaaactcag attcaactaa
    10621 atgcaagata ttccttgcac cccctgattt agaatcctct tgtgaagcca tgtcaaaaac
    10681 taagtgcatg gagagatcgt ctgcataaaa attttgacca tatactcttt ctttcttagg
    10741 tggaagacgt aaatctatat ttaaatgttt tcgtactaat tcagcactct tcaacatttc
    10801 attataagca atttccagaa gagtttgttc ggataaagcc caaaatggaa ctattcgtcc
    10861 aaatcggtta ataacttcag gctttagacc ttcaccaatc aaggcttgtt tgatccctga
    10921 tgcgactttt cggtattctc tttttagttc agtgctgtat tcattagaat tttccttgtt
    10981 tgtaacacga gatagtgagc tatatcggtt taacgaatcg aacatctccg cacctgcatt
    11041 tgttgtcgca aataaaattg tatttgacaa tgaaacggtg tcgtgaactt ctcgatcatc
    11101 ccatcctctt tcagtaaaac ttatcctacc ttcatctaaa attgataaaa aggcatcgag
    11161 tgtttctaca ccagctttct catattcatc acacacaata atcgcagaag gatcatgcct
    11221 tacatgagta cctataagtc gtctaaaacg cattgcatca tctttggttt tgtagtccgg
    11281 catattaact aagagaactt tattttcatc gccataaagt cctcttgcca cggctttagc
    11341 cattgcagtt ttaccgaccc ctgttgatcc tgtaaacaga gcagttgctt ttggttgctt
    11401 tcgaccttta aaacgatcaa atccaagctt catacgttta aacaacattg ttatagtata
    11461 aactgctaac ggttgtcctt ttatggactt taattcctct tgaagttttt ccatatcaaa
    11521 atcagaatct agctgaatat tatacttacc aaatatttgc aatagacttt ctcgattata
    11581 agcatttccg tctactcgat cttttgcaat caaatcctcc attacagcaa ttgatttagc
    11641 cggttcggac aaatcctcac gataaagttt gtttgcttgt acgatttctt gaagtaattc
    11701 atcaggaatc ctttgcgata atccaaaaga agcctgagga tcatctttaa cagaggatgt
    11761 atttagccat tgtctcaaaa tatcgacgac aacatcgctt tttagttcat tcaattctaa
    11821 cggttgtagc cgtctcccta aggcatcatc tcttttgata tagttttcat attcttttct
    11881 tgtagtagcg gtaatcattt gaagtgattc atttgctaac ggttctttta gcgaatcacc
    11941 cccaatttta ttgccactcc caaaagagct aacaacttta tgaaactcgt caatgaagag
    12001 gacaaattca ttatctttgt cctcagcttt taaaattcgc tcatattctt tcaatcttgg
    12061 aattaatgtt tctaaacgtg tttggaggac atcatctccc ccaacacgca attgtccgat
    12121 tgataattta tatacttgta ctttctttcc atcttgtcgc tgtctttcag cccattcact
    12181 tgcaagtgct gttttacctg ttccggcttc acctaacaag atagcttgcg gtgtttccgg
    12241 tctattcaag atcagtgaca actcatggag ttcgtcatca cgtcccacta acttatgttt
    12301 tggtggtttc attggtgtga gagcatgatt aaattcggtt tgaatattct taaaaaactc
    12361 ggtactattt tccatctgtt tgtgttcact tcctctaaaa taagaataat ttacttctag
    12421 tcagtcaaag ttaggtactt aaatgtcgtc ttcatgtctc caaaagaatc tttgttaaag
    12481 tcagcatttt tgtattgatc aatcaatttt tgtgtatcat tgattgcttt aatttcggtc
    12541 tccattcgac tgtctagttg acgaatactt tcttgcgaat ctttatcgtc aggattttgt
    12601 tgtaaacgtt ccagatactc agcttttgtg gcttcaattt tcttgatgtt agtatttgaa
    12661 gcatttaact gatcttctag cttatttaca acattattct tacttgtaac tgctacaact
    12721 tttgaataat caatatcgcc atctttggta agaaagcttc cttgatagac attagttgaa
    12781 tctgaatatg gatttattct gaaattgata ttatcaggag tattaacagg ttttgctttt
    12841 ttcccaaaag aaattactcc ttcattatta acatcattca tagaatattg agaaagtgct
    12901 tttgtaactg aatctgcttc attcacattg actttggtat catcgtcagc tgtatcatca
    12961 ccagaaatta gtgatacttg gtttgcaata aaaacttgat aagctctttc ctctaattta
    13021 ccttttagga ataagaaagc atccccatca gtacttaaca tcccatagct catttcaagc
    13081 ttcttaggac gatctttttc ttttgttgcc atatataagt tatagttttt tccttgcgta
    13141 gataacaact ttctagcgtt ttgggagtaa ccaagtttta ctactgttac atctctattt
    13201 tgatctgtcc acacatctga aatagtgatt tctgctcctg atcgagcaaa ctccaatgaa
    13261 gaccctatag gtgttgtatt gttcacgaga taataattat agtttcctaa ttgataaatg
    13321 tagctaccca agcctgttaa taaaaaaagc actcctagaa aagttgcaag cataatgcgt
    13381 aatccgtgac gtgttctctt atccttatca ccaagcaacc ctccaataat actgtattga
    13441 tctttggtag atgttgaatt gacgtttttt tccatcagtt tgtctgcccc ctttcataaa
    13501 agagcaaaca atcagacgtt ttttcggtaa ttctaggttc gggattttga atcatttcgg
    13561 tatattcttc cccattaata tttaacacaa aaattatctt cctttcttcg ttcctttctt
    13621 ttgctcttta ttttccaaat aatcaatgat ttttgctatt gtaccaagat agtaattccc
    13681 ctcagctttg ttttttcgga ttttgctaac cattggagta ggtacaatct cttcatctct
    13741 caattcactg aaagattttt tacctagata ttttccaata acagatttta aaaaacgttc
    13801 aaaatctttc tcgtaagttg ttttcttgcc ttttagttca tggttcatat tgtcaacgac
    13861 ttttgcggtt tcaataattg ttttagttct cagcttaata atttcatctg gaattttcaa
    13921 tcctcgtaat ttcatcgttg tactaactgg taacccatgc tttactgcat cagcaatact
    13981 aaaacttttt agatcatcta gtaagctctc catatagtta acaaattcat gccaattaat
    14041 agcattcatt gtctcacccc ttcatttttt taaaatgagg ttcgggcgta acacatttat
    14101 gttattataa tatcataata acataaataa atcaatcagc aaatttagat atcccttaaa
    14161 gtatcagcaa ttattcaaca agatatttat tttagctatt tcagtcatca taaaagacta
    14221 attagcaata aaataactaa tattatccat ttatcagacg aaaagaaaaa acttatctaa
    14281 ttactctata ttaactttta acaaatagtt gacttgtcca aaaatgttac cttcaatctc
    14341 acaaagatat taagatgttt cctaccaaaa ctgatatcac cggacttttt attctgtgtt
    14401 gtccaaaagt gttaccttta gtcgatcaac aaattaaaaa aaattgtttt tttcttgact
    14461 tgtccaaaag tgttacccat agttgttaaa atttgtatag attagccatt ctttcgaata
    14521 ttatttagac tgaaacttgc ttacaaaaaa acttcttgca tttattgtcc aaaagtgtta
    14581 cccatacttt tttaaagaag caggttgtat taaaattaca acctgcttct tctcaatctt
    14641 cttctaacca attatgcatt gaaatggttg gcaagtttga ttctggctca ctaaaagtga
    14701 atctgagccg atcaactctt cttcctttct ttgcatagat tttttcataa tcaaaagttt
    14761 caaatacctg tattccgttt tcatcaggag ctaaaaattc ttctttcgcc tgctctagta
    14821 ttcttttgtc aatatttgtc attctataac ttgaaggtat acttaatgct cttctaaaat
    14881 cttcaacttt gactacccaa taaccatttc gccactcttt atttctgtat tgcatgagat
    14941 gacgatacaa ttcttttgtg tagctacttc ttagttttgt aaaattatca agttcaaaac
    15001 gagtaaagtt tttgccaatt gagtttaaga taaattcaaa ttcctcattt gccttaattg
    15061 taactgtttc cttctgttta tctatccgga atttttgaaa aagccacagc tgttcataga
    15121 cgtctgaatc ttcaaaacgg taggttaaag tacctagttt gtcactcaaa gcttttaaca
    15181 cagaataaaa actgtccttt tctcttctgt cgtatttgac caaataacga aagaggttga
    15241 aatcaaaagt tacttctgct gttccttgac gtttcatttt tgataaaata gcccaaaaca
    15301 catccatttc aacaggctta aatcctcgca aagctgttgt attcagacca ttgttataac
    15361 gcacaattgc atttgtttgt tctgatacta aatctacatc actatcaata atcttttcgg
    15421 taacttcttg agacattgat tactctccaa tcttcttttt gcgctccttt aatcatgtta
    15481 atctggtact aattaaagga aacaattttt tctgcttgtt acctaaatta aatcacaact
    15541 aaaggtaaca gtcaacaaag atttgttact aatctaattt tctatttttt cgccttccaa
    15601 atcaagaaaa ccgaaggtaa cgtcttatcc tagtgtgata cctcaaatgt ccacttttgt
    15661 tacctttgct gtccaaaagt gttaccctta ctgtccaaaa gtgttacctt tactgtccaa
    15721 aagtgttacc tttatacccc tttaatactt actctcccaa gagattaagc ctcccctaac
    15781 agatatatta acagatattt aacagatata ttaacagata tttaataaag gaggattact
    15841 cctctcttat ttcaaaatct taaaaataaa aaatcttaaa aaaaactaac actatctagg
    15901 agtttatttt gcttgtgtaa ctttgaataa ttgaaaaaaa ggaaaaggag gaagcttcag
    15961 cttccccttt tatttatatt tataatatat attttaatct ttaaaacttt agcaaccttc
    16021 aaaaaggctt tatatcaatc ttaaatagat tttagggtgg acataaagcg atgcgaacat
    16081 ggacaaaaag cgatgtgaat gtggacaaaa agcgatgtgg tattccgaat atatccaaaa
    16141 aaagaaaaag tatgtgaatt tatttctaaa aactagaaaa atatttgttt tttaattttg
    16201 gtaattttta taatttcgta tttttttgaa aaggattttt atattgaaaa tgttctaaaa
    16261 tagcgatttc tgctgtctta tcaatgaaaa ttgattaaaa aaataaaatg tgttttacag
    16321 agattgagtt atgttataaa tttacgatac atttcttatt gtatcgtatc gaaaacaggt
    16381 ctatacttat tcaatggaat cgtaccagtt ccaaattttt aagctcgtac caagcttaga
    16441 aagaagaggc aatcgtatga aaataacaga aaagaatgta aacccgaaat ttgaacctac
    16501 cgaaagcaat ctaaaaaagt ttgatttaac tgttacttta tcgaatgaac ttgtagacag
    16561 cattgttgaa atggatgcta tgccacaaaa aatttatctt tatgctatag gacatcttga
    16621 ccctaaaaat ccgcctttta ataatactgt taaagtatct aaaaaggctt tatttgcgtt
    16681 gatgtcttct agttcatctt cttcaaaaca ttcaagatta aaacaagtta ttgaaaaatt
    16741 gcatcagcaa tctgtcatta aaattcaaag aactaaacct aacggtaaga ctgaatacaa
    16801 agtcatctca ccgctagaaa ttactgaatt caatgatttt tctgatgcag tatctattaa
    16861 atttagtagt tcaattatgc ctttgttaac gaacttagag gatcatttta cacagtatac
    16921 atttgatcaa gtagttaact tgacaagcaa atatgcgatc agtttttttc aattattcac
    16981 taaagatttt aatctgtatg aagcaaatag agataactta acatggagta caaaatcact
    17041 tgatagatta aaaaatcctg ttttctccat tgcagattta cgaaagaaat ttagtattga
    17101 gaaaaaatat aaagcttttc gagactttga aagacgtgta attaaagaac cgattgccca
    17161 actgaataaa tacactgatt ttaaaattga ctatgagctt atcaagaagg ggcatactta
    17221 tacaaatata aagttcttgc ttacacgtac catgtcagtt ccggtacctt ataagcaaga
    17281 acaaagagac cctgcttatt taaaaggcgc agaagataaa acagagaaaa tgaatcaatc
    17341 gtacttgaaa gctatgaata gcaaatacac taaattacta atcaaaaact ttttgatcag
    17401 ttctgccgac ttatgcgatc ttaaacttat gagccgactt cagaataaag tttatccgct
    17461 ttatgatcgt ttatccaaag atagaggatt tgaatcagtt gtcaatcacc tatcttacgt
    17521 tgctactcat aaggaggatt acacaataaa aaatactgtt aaatatctcg aaaaatcaat
    17581 tacgagttat ttgccttatg tgaaccttta tgattaacaa ctgaaaacat gcgtgaagga
    17641 aggaaaaact tttgaaaaaa gatgaactga caaagtacaa tgaggagttc ttagcaacaa
    17701 ttgatatgaa aatggattta aagtcgtatg aactttatca attgctgttc agttatatct
    17761 cacccacaaa gctaaagaaa acaggcgtag tagatttacc tgttaaagcg attattaatt
    17821 acatggatgt tgatcaaaat cttgaaacgg aactaaaaac catagttaac actttgcaaa
    17881 gacaagcagt ttatgaaata tcgaatctag acaagaaata taagacgtat actactgtat
    17941 ctgctattga tcggattcat tgggatttag aagagcatag tgtatctttc agtttttcag
    18001 aagatgtaat tcccttaatt gtctcaatca aaaagaagta tccgttcttt gtttttaaag
    18061 acttagcatc ttttcaaaaa aaatatagca tcgtgttata caaatatttg gcgatgtatt
    18121 ataaaaaata tcttatcgcc aaaaataaag gagattttga gaaagctgaa atatataaga
    18181 atcccctgat tgaaatagtt aaaattcgtg agattacgaa aacagaaaat gagtataaaa
    18241 caatatcgaa ttttgataga cgtgttttaa gggaatcagt tgaggaaatt aatgaaaaat
    18301 caatgctgaa gattgatttt gctaaagtta gaaaaggcag aaattacaat tttgctgtcc
    18361 agtttaatat ttctagtgag tttgatgaaa aaccttatag agaaaaaaaa ttaggttaat
    18421 gcaaaatata tcttactcgt ttgaagatgc ttcagacgta tgctaagata tgtagtgcct
    18481 tgggagcaag gtattaatct cctttagagg ttaattagct tgtgatcgcc tatcaatttt
    18541 ttggtaggcg attctttttt tgaaaccaaa aagcaa
//
LOCUS       DACEPW010000041        18234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEPW010000041 DACEPW010000000
VERSION     DACEPW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.042"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            25..1812
                     /locus_tag="IW819_001111"
     CDS             25..1812
                     /locus_tag="IW819_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAQ1244230.1"
                     /translation="MDTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLE
                     QADQTGDYSKLTPNNLVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQ
                     YGATKIGSDKAKGNILSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHES
                     LLNQRIVVEFYHKGKKFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAW
                     YEFSQKVLKDEEGKIVYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPM
                     AIFMLTPDYDPSNHIIVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMD
                     NMEGIMTVTAGRNMLWDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEE
                     FSKKVGNKTVEQENSTINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQ
                     DLKGHKIRPYPIFNHGETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYD
                     YFLPDEYARNLYHGLHDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQE
                     YVEKLIKNEEISEMIRKRLLLEAEKAVKE"
     gene            1809..4187
                     /locus_tag="IW819_001112"
     CDS             1809..4187
                     /locus_tag="IW819_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244231.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            4248..4790
                     /locus_tag="IW819_001113"
     CDS             4248..4790
                     /locus_tag="IW819_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244232.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            4868..5200
                     /locus_tag="IW819_001114"
     CDS             4868..5200
                     /locus_tag="IW819_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244233.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            5201..5794
                     /locus_tag="IW819_001115"
     CDS             5201..5794
                     /locus_tag="IW819_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244234.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            5816..7510
                     /locus_tag="IW819_001116"
     CDS             5816..7510
                     /locus_tag="IW819_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1244235.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            8198..10075
                     /gene="ltrA"
                     /locus_tag="IW819_001117"
     CDS             8198..10075
                     /gene="ltrA"
                     /locus_tag="IW819_001117"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1244236.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            10206..10583
                     /locus_tag="IW819_001118"
                     /pseudo
     CDS             10206..10583
                     /locus_tag="IW819_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            10583..11782
                     /locus_tag="IW819_001119"
     CDS             10583..11782
                     /locus_tag="IW819_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1244237.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            11798..12442
                     /locus_tag="IW819_001120"
     CDS             11798..12442
                     /locus_tag="IW819_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244238.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            12453..13259
                     /locus_tag="IW819_001121"
     CDS             12453..13259
                     /locus_tag="IW819_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244239.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            13282..13511
                     /locus_tag="IW819_001122"
                     /pseudo
     CDS             13282..13511
                     /locus_tag="IW819_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13670..14605
                     /locus_tag="IW819_001123"
     CDS             13670..14605
                     /locus_tag="IW819_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ1244240.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            14649..14918
                     /locus_tag="IW819_001124"
     CDS             14649..14918
                     /locus_tag="IW819_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244241.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            15194..15469
                     /locus_tag="IW819_001125"
     CDS             15194..15469
                     /locus_tag="IW819_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244242.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            15511..15933
                     /locus_tag="IW819_001126"
     CDS             15511..15933
                     /locus_tag="IW819_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244243.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            15953..17545
                     /locus_tag="IW819_001127"
     CDS             15953..17545
                     /locus_tag="IW819_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244244.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            17566..17901
                     /locus_tag="IW819_001128"
     CDS             17566..17901
                     /locus_tag="IW819_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244245.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            18047..>18234
                     /locus_tag="IW819_001129"
     CDS             18047..>18234
                     /locus_tag="IW819_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244246.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         6753 a         2786 c         3783 g         4912 t
ORIGIN      
        1 gtgaaatact ggaagcgagg agacatagac accgcaacac agttaaccaa tacttttacg
       61 aatacgattt attatgatgc aaatgcttca gacaataaat actttaacga aaatgcgcag
      121 aaagctgtca atgcgcttat ttttgcgtta ttggaacaag cggatcaaac aggggattac
      181 tccaaattaa caccaaataa tctcgttgaa ctactttcag aaattggcgg ttttaattat
      241 tatccagatc ctgagaatga attcaagcag ctcaatgcct tagatgagtt catgaatcag
      301 ttaccccctg gaaatatagc aaaaaaacaa tatggggcaa caaaaattgg atctgataaa
      361 gcgaaaggga atatcttgtc tacggcaatt acaggattga atccatttac cctaacgaaa
      421 attgcgaaga tgacttctca atcgaccatt gattacaagt ccattggttt tccgaagtat
      481 ttggatctca aattacatga aagcctatta aatcaacgca ttgtcgtgga attttatcat
      541 aagggaaaga aatttcacga ggaacgagtc aaagttggct atcgtggttt ttgtgaaatc
      601 aactttgatt gtcatttaga gcagggagat catgtttgct taaaatacaa tcatcaagga
      661 aaacaatatg ttgcttggta tgagttttct caaaaagtat tgaaagacga agagggaaaa
      721 atcgtttatc gaaaaaaacg aggagaaact catttgccag aactagaaaa agaagccgtt
      781 ctaacattag atgagaagaa aagtaaccta ttagtagagt ctattcggat gcactatacc
      841 gataaaccaa tggctatttt tatgttaacg cctgattatg atccatctaa ccatatcatt
      901 gttagcatct ttcttagtca gttgtataaa gaattatcta cgcaatgtgt taagactaaa
      961 ggagataaat gtcaccgtag gattcatttt attttagatg aatttggaaa tatgccaccg
     1021 atggacaaca tggaaggaat catgacggtt acggcaggtc gtaatatgtt atgggatctg
     1081 tttattcaat cttatcagca actttatgcg aagtatggag aacaaaacgc agcgattatc
     1141 aaagaaaatt gtcagacaca tatttatatt atgtcgacag atgataatac catcgaagaa
     1201 ttttctaaaa aagtggggaa taagacggtt gaacaagaga acagtaccat caattcatta
     1261 ggtatgaata ctcatatcaa tcgcaatgtt gattcagatc gtatcttaac tcctgaaaga
     1321 atttctactt ttttagaagg tgaaacgatt gtactcgctc ctttaaaacg acaagatttg
     1381 aaaggtcata aaatacggcc gtatcctatt tttaatcatg gggaaacgaa tcttccttat
     1441 gcttatcaat atattgaaga tttcaatcca tcgtgggata tcaatgatat agacatacat
     1501 tgtacacatg cggacttgga tcttaaggct ttacaaattg attatgatta tttcctacca
     1561 gatgaatatg ctagaaatct ctatcatgga ctgcatgatc aattagaaga aaaaaacgaa
     1621 gaaaatatgt tttataaaat cgttcaatct tttgagaatg agacggctaa aaaattgtta
     1681 attcaatttt acgaagaaca agcaacggat cgtcttcaag aatatgttga aaagttgatc
     1741 aaaaatgaag aaatttctga aatgattagg aaacggctgt tattggaagc tgaaaaggcg
     1801 gtgaaagaat gatcattgga ggaattttct ctggcatagc cgactggtgg tcaggtttat
     1861 tcggagaagg ggatgcagat cccaaaaccg taacgaaagt cttacaagat tatggtaatt
     1921 atctgcagta tcaagatgta ttcagttgga tttgtaatac ttttttatgg ggattgatca
     1981 aagggttata taatctcaat actttattag aaaaaacaat ttatcaaagc tttgatttaa
     2041 aagacttatt gaacgccgct ggtgttaatg aactatttca aagtttgatt agtaaggtat
     2101 tggctctttt ttgtgtcgtc acattaattt atttaggctt gaaatttttt atgagtaaac
     2161 acccaccgaa agtcaaaaat atcttggtta atttatttat ggccatgatc ttgatttttg
     2221 gtggtagttc tttaatcgat gaagggttaa atatcagtaa aaacttttat ggagacgtca
     2281 ctgcagcaaa taaagcagat aaaacgggtt cacctgcttt tcaaattatt caaaataatg
     2341 tgattgatgt cagtacatta ctagcgactg acccgaataa agtagaatcc ttaccaacag
     2401 ataaaagaaa tgctttaaca gcgaaaaatt tcaaaacagc gaatatcaat gggattatta
     2461 cacctgatca agctaagaaa atgtctgaat caagtgatat tagtacagta caaaaagatc
     2521 ggttaaaggc attacagtat cgactagagt tagacgataa tgggaaagaa atccctgttc
     2581 aaattagtga tgaaggttta gcaaaatatg tgtatacgtc aggctatcgt cgatacatta
     2641 gtagtccagg gattattttg gctggggaat cttctttgtc ggtagcctat ttatttattt
     2701 tgtttacaat cgttacttgc attattgagc tagtatttaa aaaattctat ttagtaattg
     2761 ccgcgtcaac ggactttgag acaggacaac ggatgaaaag tgcgatagaa gatatttctc
     2821 aatccttctt gttattggcg tttacgattt tagaattacg aatctataca ttaatgcttt
     2881 caggtatcgg tgatttgcat gcggtaggaa agatcaatgg attcttgtat gttgttgctt
     2941 taattgtgct aactattgcg ctattcaaag gctctcaatc ggttacgaag attttcggtg
     3001 ttgatacgag tctgaaaaac ggcagcaatt ctcttcttag catgtttgcc ttaggaaata
     3061 ttgcgaaaaa tgtgggtagc ggagctaaaa accttgcaag tgctggtaag agtggtttaa
     3121 gccatttaaa tgatattcgt aagaatggat ttggccatcg aaaagatatt acccatgata
     3181 acggggaaga acaatcagac gataaagcta caactgtacc taatagtaag aagacagttt
     3241 ccgaaatgtt cgggaaagcg aaaaatggtg tttctaaagc cgcagaaggt cttggttacg
     3301 tcaatgaacg tgggttaaaa ggaacagttg aagatcttac agaaaaagcc aaagacacct
     3361 tagaagagaa attagacggc acgagagcta aagaatttgc tgaagctgtg aagaatccat
     3421 ccgaagcact agaaaaacaa aaagaaaaag cttcagcagt caaagaagga ctaaaagatg
     3481 cgtatggtga aggacaagtc aatgcagcag tgaaggcaaa tacaacaagt gaaaaagaaa
     3541 atcaagaagg tgcagcgatt ccttcagcca aagatcacca agatgagtta cattctaacg
     3601 aaaattcgat cggagaaaaa gattcagaag aaggacaaca tggtcagtta ccaaaaaccg
     3661 atccagaaca atatcgttat aaatctaaca ttaattctga aaacgaaaaa cgcatttcag
     3721 atttaccaaa acgaaatcaa gaagtacaaa cggatgagga ttccatcgat ctgccaaatg
     3781 ataatggcat cgaagttgga actgaaaaat caacgaaaat tggtttgcct aagacacaaa
     3841 atttaccaat gaaagatagt cgtttgaatc aattcaaaga gaatgatcaa agctttgttc
     3901 atcaatcacc tgcaggtact gttcaaatga aatcaagtcg tttgcctgaa aatggaaatc
     3961 tgacaactgt ttcttacaat ttagctgaaa gtggtgaaac agagaatgcg tccgttcaaa
     4021 atattgtgac aaaagaagct gcacctaagc caatcaataa agttcataat ggatacagac
     4081 cagcaaacgt agcatttact gcaccgacaa aaataaatgt gacaagagaa actaaaaaca
     4141 ggaacaatga attgaaagaa caactattga aggatatcaa accataaaag gcctatttaa
     4201 gggccttttt ctttataaaa caagtttaaa atggaaagga gaaaattgtg gaggatattt
     4261 taatagctac gtttataact gcactgggag catttttaac gatatttgtg gtgactatta
     4321 aaggatttaa agatacggat agtttacaaa aagatcatca gtctatttta gatggtcaaa
     4381 aaacacatac cgaaagcatt attgtacacc tttcagaagg attcacttat ttgaaagatg
     4441 ctatgcccac attcacagta aaattaacgg aaatatttca ttctgttgag caaatatcgt
     4501 cagaattgaa agagcaaaga caacaaaaag atcgtcaact tggctcactt aattttaggc
     4561 aagaaacgat acaagaatca ttggagaatg taaatcgatt aaacgatgaa atgcaacgag
     4621 ttcatttgga aaatgaagaa ttacgcgcac aaaattatga tttaattcaa aagaaaagaa
     4681 atctggaaca aaaaattgtt gaattaaaag aagaaaatcg tcaattgaat tatcaattac
     4741 gacgaggaaa aaatcgttct cgtgatttag gaaatgaatt agagttgtaa tatttagaaa
     4801 aacgctgtga gaaatcacag tgttttttat ttctaaaaat acaaaatata aggggtatgg
     4861 ttatattatg gataaataca ttcatatgaa aaatcctcca agatttagtg gggaatggaa
     4921 gtttttcgcc tttttctttt tagatctatt aacgcttggt ggctgctttt tttgggcgaa
     4981 aatagtcaat aatcttgtcc acttaccact cttttttgca ctcttgaatt atggattatg
     5041 gcttgcgata gcgttagtga ttgtttggcg accctataat aaccctggtc aacgccaaat
     5101 ttttgcgatt atcgaatcta ttttctttac ggataagaac cactatcatg cgttagatcc
     5161 taaccgaaat tatcatggta cgtataagga ggatgaataa atgcgagtca aaggacaaaa
     5221 ttcagttgat aagaaatcca ttttgcagtt tggaaaacca gaagagcgct ctatcttgga
     5281 cttatttcca tataaatcct gtacagaaga gggagtctta attactagag aagatcattt
     5341 tcagcgttat tatcgtgtcg tttccactga cgtggaggga ttaaatgaac aagagaaact
     5401 agaacgaatg aatcagttga ccaccgtcat gcgtacgtat gtgccaacaa ttaagttagt
     5461 tagtttgacc acagagacgg atctaacgga acaaatcgtg caaaagcggc agttattaga
     5521 taaaaatcgt atggaacagt cactaggaag aaatctacag cgattaagaa aatatgaaaa
     5581 gaaattagta gaggagatcc aggaattaaa acgggcagaa caagaacgtc ctgacttaag
     5641 cttctttttc gttattgaag gaaagacaat caaggattta caaactagaa atcgtcagtt
     5701 aatgcgatct agtggcattt tagggttaaa accactctac aaaaaagaat tgattaagat
     5761 tctttatcga atgaacaaca tgaatgacga ataaaaaaga tggaggaaat gattcatgct
     5821 agatgtgata aaaacagctt tttcaacgca aagtaatcaa aaggtgattg atccattaaa
     5881 acaacttggt aatcgtgaaa tagagatttt aaaatcaaaa ggttatgatt tagatttctt
     5941 aaaacaagtg atgccacaag gtgggttgaa ttttgatgaa gaatatgtga cctatggaaa
     6001 tggttattca cgatgtgtga cagtttatca ttacacaaaa aatcctactc ttttttggtt
     6061 ggccaatctc atgaacaatc ctcatacagt ggccacacta gatgttagca cagatgaaaa
     6121 gcaaaaagtg attgatcaaa tcaaccgcgc attagatgaa ttaaatgatc gatcagataa
     6181 tgaacgaaaa gcgactgacc gaaataaaag tgcggaagaa tacctggaac tagcggaata
     6241 tgcccgaaaa ttgacttcta atggggaaat ttctaaacaa gtcaaagtga gagtacatgt
     6301 ttacggaaat acgatccaag aagtggatga acgggcgcag gaactgatcg aaaatctaaa
     6361 agggatggat tatcgtgcgg tgacttatct gcataaaacc aaacgagatt ggcaatcgat
     6421 gtttaccatg ttcgatgaac aagctgaatg gtttggaaat atcaaagggc aatcacttcc
     6481 atcgttaaac attggcggag gattcccgtt ccaccaccaa acgctaaaag atccaggagg
     6541 cacacaatat ggggaaacca tgacaggcgg cccatttgtc tggaatgcca cgtataaaaa
     6601 tagtgtacgt ttatctacca ataccttgct tctaggaatg atgggagctg gtaaatcgac
     6661 cgttcttaaa atgatcgcgg aagcccattt agctgcagga gactttattt ggggctttga
     6721 aaagggaaaa gactttattc ctttcttaaa ggaatataac ggtattatgg tgcgtttaga
     6781 cggatctgac gggatgatca atcctttaga gatctttgcc acacggacgt atgatgaagc
     6841 atccagttta tatgacgatg gttcagtcaa agatttgaag atcaatgaag cagcgtccta
     6901 tcaaacgcac ttagacaaag tagtctatca ggtacagtta gtgagtcctc agctaaaagg
     6961 aacgatgaaa gcagaattca agacctactt gaatcgtttt tatgaagagt atggaacagt
     7021 accacgcggt tttacttcct caaattcgcg ttcaaatact gatacgcaag tcacagggaa
     7081 agatccagaa gcttatccga cgttcaaaga atttttagcc tttttaggac aactggaact
     7141 accaggggct tcccaagaga aaaagaatcg gaaagaagaa atggagtcta tcgtggaatc
     7201 cttatgtgag acttatggca tgatttttga tggtcatagt acgattcgac accttgacca
     7261 acaacagttg gtcttttttg acattgactc tattacaggc ctagatcgag gggtacaaca
     7321 atgtcagatg tacatggcca tgacgttgat ttggaatcag gcattgattc aaggacgaag
     7381 acaacgacgc ttaattaaag aaaaacaaat ggaagaacaa gacttcaaac gcttcttggc
     7441 gatgattgat gagtgccata atggtgcgac ctgttctgca gtgaaaagct gtgggttgca
     7501 agtagtctaa aaactattgt aataactctt ataacgagaa gtcaaatgag tgggtaacgc
     7561 cacaaagggc ttccactgat actccgacat gcaacaagta gtttatgcag accaacgttg
     7621 tgtgaagctc ggtgaagtcg gctgaaggat accgtaaggt aaaagaaata gtgaatacca
     7681 cgaaagttct ttagaggtaa agggtgtatc cgataagtcg ggtgtccaaa aaaacttaat
     7741 atggtgagta tgtacaaacg tactgacgaa ctagcgaatg tactgtccga aaggcatagc
     7801 tcctagaaat gggagagaca gtgagcaaaa aatcactcat tgtgctaact tgtggttgag
     7861 taaaagtcgt tgttatgaaa gaccatgctg cgttacaggc gtaggagaag ttagggggca
     7921 aaagctagca tctaagttaa ggcatgtgga tagatataag ggaacagggg aagccatcaa
     7981 catggagagt gcatactcga tgaagtggta gtaagaaata tgtgaatgac aaataactta
     8041 ctttagtgat ggtgaaagta gagtcacgac tgatgaaggt tctgtaatgg aactggagga
     8101 acaggctcga ttcgtctgta aaagtcatga aacccgttca accatgggat tctcgtatga
     8161 ctaaaaaggt cacgagccaa ttggagcgtg ataaaccatg acaaaagaaa caagtccaaa
     8221 agataggaag ttgcggcatg cagaatatta tggcatgacg gaaatcttcg ataagatata
     8281 ctccaaaagt ttgaagggag ataatttcaa gaatctaatg cagattatca tttctgataa
     8341 caacattgtc ctagcatatc gtaatatcaa gagaaatcac ggtagtgtga caagaggaga
     8401 agatggagta acgattaaag atattgaaaa tctcaaccgt aatgaattcg tagaaattgt
     8461 taggaagcga ttcgcacatt ataaccctcg gaaagtaagg cgtgtagaaa ttcctaaata
     8521 taatggtaaa ttaagaccgt tgggtattcc atcaatatgg gatagaatcg cgcagcaatg
     8581 catcttacaa gtattagaac caatatgtga agcaaaattc aataatcaca gctatggttt
     8641 ccgcccaaat agatcagcag aacacgcaat agcagattgt atggcccgaa tgaatcaaag
     8701 tcacatggaa tatgtggtag atgtagatat caaaggtttc ttcgacgaag tcaatcatac
     8761 taaactcatg agacaaatct ggtctatggg tatacgagac aaacagcttc tcttaatcat
     8821 taggaaaatg ttaagagcac ccgtagtact tccaaatggg aagttgatat acccaacaaa
     8881 aggaacacca caaggcggaa tacttagccc attacttgcg aacatcaatc tcaacgaatt
     8941 tgactggtgg atagcaaatc agtgggagga aaaagatgcc aaagagctaa aacatcattt
     9001 taggaaaaat ggagcttggg ataaaggtaa tatctatcac aagctcagaa agagtacaac
     9061 tctaaaagaa atatacctag tgagatacgc agatgatttt aaaattttct gtagaaatta
     9121 taatgaagcg aaaaaagttt tctatgcaac taaaatgtgg ttacaggagc gtctaaagtt
     9181 accaatatct gaagaaaagt caaaaatcac aaatctttca aaagaagaaa gtgaattttt
     9241 aggctttacg atgaaattag tgaccaaagg taacaaaaag gttgttaata cgcatatagg
     9301 tacaaaagca caaagcatga ttaaagtgaa actaaaaaaa caaatcaaac gaattcaaaa
     9361 aagtcctaat agcaaagata cctttatcta tattggagtc tataacagta tggttattgg
     9421 gattcataat tacttcaaaa ttgcgactca ttgtagtgta gcaatgcgaa aaattgcaag
     9481 agaactcaga ccaagttttg aaatacgact acgagaacaa ggaatctcaa aagaaggaca
     9541 atacggaaag tacgaaggat acaagcctta cctacggtca ggacaaatac gatatataag
     9601 agggtttcca atattaccag tagggtatat tcaacacaga catccgatgt gtaaaaaaag
     9661 aaatattaat aaatacaccg aagaaggtag ggaagccatt cataagaagc aacaaaccgt
     9721 agaagaatgg aagatatcgt ggttgtgtag caatccagtg cttagtaaga gagcaacgat
     9781 tgaatttgcg gacaatcgaa tttcaaaatt catcgctcaa aagggaagat gtgcagtaac
     9841 aggtgaggag cttatcttat ctgaaatgca ttgtcatcat attatccctt atcatgaaag
     9901 caaaagtgat agctatgaaa acctagtgat tgttactgag gaggttcatc gggtaataca
     9961 cgctacacaa agtgaaacga ttgaggaact tctaaaatat ctaaaattaa atccaaaaca
    10021 aaaagaaaaa ctcaatgagt tgagattaaa agttggtaac gaagaaatat cttaataaat
    10081 atttgtagga cgggaaaaca gaatgtcttt tatagacgag ggaacgccgt atgcggtgaa
    10141 agtcgcacgt acggtgtgga ctgggggaaa agacggagat aatatcaaac tcttacctat
    10201 cagtatcgat taatccagat ttcattgaaa cggtaaatta tgtcacaaac ttccaacgag
    10261 aaatgcgtaa agtagaagca atgactgttt tagctacaca atccccacaa gaaatggtgc
    10321 cagaaaatat gtcttctgat aacttctcga aactgaagaa agtctttgaa ttttcttcta
    10381 cgaagatctt tatgcgtatg gatgaatcta ttttacgtca tatagaagga ttagttggta
    10441 aatcaatgac gaactcagaa ttagaatcca ttcctcaaca aaatcaagga gacgctgtga
    10501 tcaattttgg ctcaaaagaa accattcgtg ttcattttag tccgaacaaa cgtcaattaa
    10561 aattgtttga tggaggaaaa taatggaaaa ccaaaatgaa agtttaatca aacagtatgt
    10621 taagcgtcgt gcaaagcggc gcttattttt gtgggtgttt ggtacttctg caggtttgat
    10681 tactatattg atcactgtgt ttgtgaccct ttttctaata cttgcggcag gttctattga
    10741 taattctgat tcaagtagtg gtggcgaagc ttttacagga gaatattcgg aaggcttacc
    10801 catttataaa gaaatcaaag gaagaggccc tttttctgat gagattgcgc aatatgctgt
    10861 aggtgcagca gtgaagtata aattattacc aagtgtgatc ctttcgcaat acggttatga
    10921 atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc aattattttg ggattacgtg
    10981 gtttgatggg tgtctgtttc ctaaaggaac tgcgcgtgga attggtggca ttgaaggtgg
    11041 ctggtacatg aagtttccca actcaaaagc tgccttctcc tattacggct ttatggtagc
    11101 cacacaatca aattttaatg cttgtgtcgg aaataaaagc cctggcgcaa gcttattgat
    11161 cttaggacga ggagggtatg ccgccgcagg aattactgaa gacagtgcct attataaaaa
    11221 ttgtatgtct attattaata caaataaatt gatggagtac gatgagtttg cgattaaaca
    11281 ctggagagaa ggcggcggaa ctggtggtac gattacagga agctggggca atccattccc
    11341 aggaagtagc ttagataaaa attcgttcag tggaggacaa ctttttggga aaaatccagg
    11401 aggagaattc cgacctaacg ggttccatga tggactagac tttggttcag tcgatcatcc
    11461 aggaagtgag attcatgcgg ttcatggcgg aaaagtcgtg tacgttggca atcctggtat
    11521 ttcaggtttg ggtgcgtgtg tgatcgtgat taattacgat ggcttgaata tggtgtatca
    11581 agaatttgca aatagtacag gaaattctcg agtgaaagta ggcgatcaag tgaaagttgg
    11641 tcaagtaatt gggatacgag atacagcaca cttgcattta ggatttactc gaatggattg
    11701 gcgtcaagca caaggacatg cattcattga tgatggtaca tggatcgatc cgttaccatt
    11761 tttgaatagc agcaaaaaat agatcaggag aaaaaatatg gaaaataaaa ccttaaaaat
    11821 cgtcttaaca gctgcaatta gcgttagttt aatcggcaat attgcgttgt tttggcagct
    11881 taaagaagaa caagagacaa atacctataa aattgaagaa gtggctaaaa acgcgtctga
    11941 taagctcaaa aaaaattctt ctgaagcaca ggcgaagatt gacaagttaa atcaaaccat
    12001 caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa gatggcacaa aagcaaaaga
    12061 ggaagtaact gaagcacaaa gaaagacggc agaagagttc ggtcaattgg ccattgataa
    12121 atcattaagc agaaatgaat taacggaaaa actaaaagat gtcgcaaccc aagaagtgat
    12181 cgacaaatta agtcctgtag acgatgatca ccaacatgat gattacggaa gttactcttt
    12241 tacattaggt gacgtgcaaa cttacgctca aacaagcagt gtaaaagagg aacaaaattt
    12301 tgtcgtattt gttgattata caatcggcaa tcctcaattc aaagatgtca aaccacaaaa
    12361 aataaaaggt ggattaactg tcactgaaaa acaagtagat ggtcaatgga aagtcacaga
    12421 ctttagttat ttcactcgtt aggaggaaga aaatgagtaa gaaaaaagga gcaagacaac
    12481 gttttaaaaa acgaaaaagt tatcttagat ggctggggat ctttatgatt ctcggctttt
    12541 tgattcttgc tagtccgatt tacacacata aaaaggtaga cataccagat gaagtaagca
    12601 cgaacgcatt tttaaacacg aaggaactga cgatggtttc aaaagaaaaa ggagcgaacc
    12661 agcaattagt ttttgaattt gcgatcgacg atgaagatca gagcttatta aaagaactag
    12721 ctaatcttaa ttatcgtgta gaagtaaaga cagctaaagg ggattatcaa gcaataaaag
    12781 ccaaagtgat caaggtgtct gatgattatt ttgtagtgaa gttagcacat gtaccagaag
    12841 aatatattgc tatgcgatta actattattc ctgaaaaaat tgatccaaag gtggatatgc
    12901 aagaaccgca agatctgatt ttttatattc acgaagataa ggtcaaagat cacgtgaaag
    12961 acgaagatta tgaacaacat gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa
    13021 aagcagcaca aaaacaaatt gaatccttcc aagcaaccat tgatttgaat gaagaattag
    13081 tgaagaaact aaaagatcaa ttgccttatc aagtcgcaga agatcaggaa aatactgaaa
    13141 ataagattaa tagttaccag caagaaattg aaacatcgaa aacgcaaatg aaagatcaag
    13201 aagagagcat tcaaaaactt caagaaaaaa ttgatcgtat gacaaaagaa aatatttaga
    13261 agaataggtg gagtaaaaga aatgaagcga gcgaacttgg tcggcaaaga tactttcgct
    13321 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcaa
    13381 gaagtgaatt atctaacaca acaatatctg agttgaagaa tggtagaaag agtttagata
    13441 ccttaaaatt cagatcattt gaagctctat atatagctgc taaaaaatat tttacagctg
    13501 ataataaata aaaaattaag gtgggttcta tgtgaaaaaa acgtatcaaa agtttttatg
    13561 gattagctat atcgtcctat ttatcgttgg gtttctcgtc agtgtgtccg tgatcgtcgg
    13621 aacgcttttt cttttgttct ctgacgaaga tctggtactt aaggggttaa tgagtacgtt
    13681 atcgtgtgtc atgttacttt atttatgtat acagatcaac gaaattatgc cccctcattg
    13741 gcaagaaaag aagaaagaat ttaaggaaga aatcgaaata aaatccaatg actggattga
    13801 atacacatgg cttcctctga aaagaaatag caaacgaaag gaattttggg cattgcaatt
    13861 atggccaata ggaaaacttt ttatttatat ggcttcactt atggttactt taatcggtta
    13921 tttggtggtg gtctatgggg tggcctatta cttgttaagt gagccaaaac agatcgcggc
    13981 gtcaaatatt ttcacggcag taatgacatt aatgtgtgtc atgtgctttt ctatttttag
    14041 ttacttactc gtccaattta tcaggcaaaa tttaacaatg agtaaacttt tcaaaagttt
    14101 attggactta cttatcttag gcagtatgtt aacgactcta acagatcggt tcgaacaatc
    14161 tgaaaaaacg gtagagggga taaagaactt cttaacgacg attgatcaat ttataaatgg
    14221 atatatgtat ttgtttgaag taggctttat tctcactttg tttggttgtg tatttataaa
    14281 actcattaaa agatcaatcg aaaaagaaat ggttattgat ctaattccga cgctgagcgg
    14341 ctatgagtat tacgttgatt ttggaaagta tgaggagttg atccatacgg atatcgttca
    14401 cacgagtatc catgaagtaa ccgttaaggg catcagatct actttaaaag atggacagga
    14461 gacgaaaaca atcgaccgaa gaaaagaggt ctacaatttt tataaagttg aagcaagtct
    14521 acgatatggc attgggaaat tgaggttaga tgggagtaat attcaacaaa acaaaccata
    14581 tgttttagga aaagtggtga tttagaactt cctatgaaag atgaataaat aaggagaatc
    14641 ttttgaaaat gattcttctt tacagaattg tgcttatagt tggcacggag ttaaaaatct
    14701 taaagaagga gcagttattc aaagtgaaag aacatcataa tcagttacta tgtattaaaa
    14761 cgggtacgat gaacattcaa taccgaacag aatatttaga attaacgaat caaactgatt
    14821 ttttgtcgat tcgagagcaa cataagcaag aaaaattaat ggctacttat agccaattga
    14881 ttgatgagtg ggaacaattc aaggaatcta aaacataaaa ttccatttaa acaccttatt
    14941 ttttaaagag cataaggaaa cgtgtaccca ctttcaaaat aaagtgggta gataggctgt
    15001 gcctatcacg tttctgaaaa aaataaatga ccccaaaatt ttcaatttat gtgtaggttt
    15061 atgggtagca aaaatatgaa tgtgggcagc aaaaagttca tttgtgggta gtaaaattca
    15121 aatgtgtgta gcaatgtgtg tagctacaca cattgctaca cacgctataa catatagtga
    15181 aaaggagata tttatggaag taaaaataag agatattgat gaaaaaacga ttcaaagaat
    15241 tgaccgaaga tgtattgaat taagtgaacg caccgggact aaatggagcc gaaatcgata
    15301 cctaaaagtt ttgattgaaa atgattttga ccatgaactt atggatcata gaaaagatca
    15361 atttgatcgg ttgttagaaa aatttgtgga tattcaaact tataatacga aaattttaga
    15421 agaatatatt gcaacaaata atcaattaat cgccttgtta attgaataat aaaaatagta
    15481 ttgtgaattt ataaaaggag gaaatcaatt ttggcaaaag caacaagaaa acatgtatat
    15541 ctttacgata tgcatatcga agtacttgat gaaataattt ctactcatga agatatatct
    15601 aattatagtg aagcagtcag acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa
    15661 ataaatccaa ttcaaattaa attgaatgca atgggaaaag aaatttctat attaaccgaa
    15721 atggtttcag agtttgcgga tcttcatttg aaagatacag gaatactaat tggtacaaat
    15781 tcaactgtct atcaagaagc aaaagagcgt gtggaaggga aaataaaagc gaatcaaacg
    15841 aaaaagtatt ctcctaagat caagaaacca ataaatgaat ctgttcatgc cagagtagac
    15901 ccgtttgatc caacaaagtt tttaaaaaga taaaaagtag aaggggaaca tgataaataa
    15961 atcgcctgca gtcattctca cttgtcaatt taccttgccc aataaaaaat ctttttcaac
    16021 atacattgat tatatgacaa gagaaaaagc gctggaagaa aaggaacgac gcacgccgga
    16081 cgaagaacag gaactgcaac gaatcaagaa tgctttagag gatttttata ttcctgaagg
    16141 agaaactttt tcggagagta agacagataa agaattcagt gaaacgtcga atgaagctca
    16201 atcgttgatg gaacatggaa tcaattttga caagttagag gaacaagatt ttaccaaata
    16261 tctttcttat atgacccgtc actatgcgtt agaacaaaag aaagaactga cgcaaaggga
    16321 acaaaaagaa taccaacggt tgaaccaagc aatgaaaaaa tatcaaacag agccacataa
    16381 aaaagcttca ggaaatcttc ctggagtttt ttctatggct tcagacgaag tgaaaggaag
    16441 tgatctgaag gagatcaaac aaatcttcca aagagggcaa caaaaaggct caatcattta
    16501 tcaagatgtt gtctctcacg acaattcgta tcttgaaaaa atgggcttgt acgatcccaa
    16561 aacagatacc ttagatgaag aaggattaaa agcagctgga agaaaaatga tggagacatt
    16621 attcgagaaa gaacaattga atgaaacagg ttactggatg gcaacgattc accgtaacac
    16681 taagcatatc catattcatt ttgcagttgt tgaaaaagaa aatacgcgta aaccttacac
    16741 ggaggttgaa aatggtgtga gttatatcgt accgaaaggt aagagaagac aagcaacatt
    16801 agacgcaatg aagaccaatt atgtacatga gttagaacgt ttttcttatg aaaaggatcg
    16861 aatgattggt atggagaaac gaaatttatt gacgcggaaa agtgatttgc gtaacacctt
    16921 aacaaaagtg gtaaaagagc cagcgcgtta cgatcgacac gcaatgaact tattaaatga
    16981 agtatataaa accctacctg acaaacgttc ggaatggcat tatggggatg aaaaacgaac
    17041 caaactttca ccatacacaa gagagaaact cgatgagtta accaaatatg tgttaacaaa
    17101 agaacctgaa tatcaagaat atatacagat ttcaaaatca ttgaaagaag aagcaaaaag
    17161 tctttatggg gaaagcaaga gagaatcaaa ggatgcagaa aaaaatgctc tctttgattt
    17221 gaagaagcgc acaggaaatg ccattctaac agaactgaag aaacaggata gtcaaataaa
    17281 acctttaata gaaagtatgg atcagatgca atctatttac aatgaacgat cctttcgaac
    17341 gcttaaacag ttaccaacga atgaatacaa atatagttac gaagaattca tgaagcgcag
    17401 gaaacaattc caacaaagag ttgtcacgca acggagtttg cgtcaattaa atcgaacgat
    17461 taatgcccat gaagaaaaat accacgcaag taaagcctac gaagagcaac aacgaagaat
    17521 tcaaatggaa caagaaagac agtaaagaca gtaggaggaa tagcaatgaa acaatattta
    17581 gatcaatgga aagttataga aggatcatta agagaagaac gaatcgaaca acttcccgat
    17641 tgtttagaaa aagagcattt gtttcagata agagaaatgt tgagaaacga acagtttgat
    17701 ccaaatcaat ttttagtcgt tgagtactca gctactggtg tgtactgttg taatcatgtg
    17761 aagggtgaaa aatattttat cattcaagaa tatgagggca agttagcgcc ttattatact
    17821 acttgggaaa tgaatgaaga ggggatcaat aacttcccgt gtaaaagcat tgaagagtct
    17881 atttcactca cagaatgtta gttgttaagt gaataaaaga agtaaaagag tcctgaacgt
    17941 aattgtccag gactcttttt ttcaatgaat tgttttccgc caattcaatg agtggtgcga
    18001 tttgcgacta cattatatca aaagaaagag aagggaggaa gaagaaatgg cttattcaac
    18061 gattggagtt gttgttgaaa aaagcagaga caatctcgtt tttgtgacag aaattcaaac
    18121 gggcagagct tttgtcgtca cagacaaagc ggcaaaagcg tatcaaagtg gcgatatttt
    18181 aacgttaaat ctgactacga agacgttcgt cgatgcggca gaagattatc catt
//
LOCUS       DACEPW010000042        18096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEPW010000042 DACEPW010000000
VERSION     DACEPW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.043"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..145)
                     /locus_tag="IW819_001130"
     tRNA            complement(75..145)
                     /locus_tag="IW819_001130"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Gly,seq:ccc)
     gene            262..1014
                     /locus_tag="IW819_001131"
     CDS             262..1014
                     /locus_tag="IW819_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1244247.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            1030..1509
                     /locus_tag="IW819_001132"
     CDS             1030..1509
                     /locus_tag="IW819_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1244248.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(1760..3340)
                     /locus_tag="IW819_001133"
     CDS             complement(1760..3340)
                     /locus_tag="IW819_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1244249.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(3333..4076)
                     /locus_tag="IW819_001134"
     CDS             complement(3333..4076)
                     /locus_tag="IW819_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244250.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(4252..5028)
                     /locus_tag="IW819_001135"
     CDS             complement(4252..5028)
                     /locus_tag="IW819_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1244251.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(5276..6112)
                     /locus_tag="IW819_001136"
     CDS             complement(5276..6112)
                     /locus_tag="IW819_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1244252.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(6123..7052)
                     /locus_tag="IW819_001137"
     CDS             complement(6123..7052)
                     /locus_tag="IW819_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1244253.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            7244..7597
                     /locus_tag="IW819_001138"
     CDS             7244..7597
                     /locus_tag="IW819_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1244254.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(7671..8327)
                     /locus_tag="IW819_001139"
     CDS             complement(7671..8327)
                     /locus_tag="IW819_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1244255.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            8553..10487
                     /locus_tag="IW819_001140"
     CDS             8553..10487
                     /locus_tag="IW819_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1244256.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(10573..11796)
                     /locus_tag="IW819_001141"
     CDS             complement(10573..11796)
                     /locus_tag="IW819_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1244257.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(11790..13688)
                     /locus_tag="IW819_001142"
     CDS             complement(11790..13688)
                     /locus_tag="IW819_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1244258.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(13682..14800)
                     /locus_tag="IW819_001143"
     CDS             complement(13682..14800)
                     /locus_tag="IW819_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1244259.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(14883..15716)
                     /locus_tag="IW819_001144"
     CDS             complement(14883..15716)
                     /locus_tag="IW819_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1244260.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(15709..16626)
                     /locus_tag="IW819_001145"
     CDS             complement(15709..16626)
                     /locus_tag="IW819_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1244261.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(16649..16828)
                     /locus_tag="IW819_001146"
     CDS             complement(16649..16828)
                     /locus_tag="IW819_001146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244262.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(16853..17977)
                     /locus_tag="IW819_001147"
     CDS             complement(16853..17977)
                     /locus_tag="IW819_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1244263.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         5670 a         3559 c         3029 g         5838 t
ORIGIN      
        1 aaaaacataa gaagcttttt tgcttgacta ttcttattac ttgatgcagg gcagcttttt
       61 cacagcctct tttaagcggg tgacgggaat cgaacccgcg acatcagctt gggaagctgg
      121 gattttacca ctaaactaca cccgcgatac aaaaacagta tacgcctctt tcactccttc
      181 ctcaagtttt tttattcttt acgcttccga tatgttttgt agtaaaataa aactaatcaa
      241 tcaattattg gagaagatgc tatgacggga aaagaattag atctgttgct ggacacttgt
      301 ttactcgctg ggaaaatcat gatggaaagc aatgcggaga tgtatcgggt agaagatacg
      361 atgagtcgaa ttgctttagc ttctggaaat ttccgtttgg tcagctatgt cactcaaacc
      421 ggtctcttta ttggtcttga tcgtacctcg accatccgaa tggaacaaat taccaaccgt
      481 tcgattaatt tagaaaaagt agtaaaagta aatgatcttt cacgtaaata tgttgcttca
      541 gagctaacat tagaagaact ttactgcgaa ctgcaagcaa tcgaccgaga ccgttctttc
      601 tttcctgtct ggttgcaaat cgttagcgcg gcagtgatca gtggaacaat tatggtgtta
      661 tttggcggaa agatgaccga tttacctgtg actttagtca ttggaggatt gggatacgca
      721 gcttatctat atagtttgaa attttttaga atcaaatttt tatcggaatt tttatcttct
      781 ttattgattg gcatagccgc tatctacagc gtacgttttg gcttaggaag caatcaggat
      841 ctaatcatta tcggatgtgt gatgccttta gttcctggag ttcaaattac gaacgctatc
      901 cgtgatttgc tagctggaca ttatgtctca ggagtttctc gcggaacaga agcgatgatg
      961 actgctacga tgatcggttt tgcgattgct tttattttcc agctttttta ttaggaaagg
     1021 aacacaagta tggggacgat atttattcag ttttctttta gttttttagc gactgcggca
     1081 tttgccgtta ttacaaatgt acctaggcgt tcattgattt gttgtggtct ttctggtgct
     1141 tttggctgga tgatctactg gatctgtgtt caaataggcg gatctgctgc ttttggttca
     1201 ttgcttggtt ctttaggagt agcattcatc agcgatcttt tctctaaacg tttgaaaatg
     1261 cccgtaacga ttttcaatat tcccgggatg gttcctctag tccctggtgg tttagcttat
     1321 caagcggttc gaaatctcgt tactgaatcc tatcgagaag cggctcatta tgcggtccaa
     1381 gcaattatga ttgctggagc aatcgcttta ggtcttgtat tatcagaagt ctttaaccat
     1441 aacatccgta atttcaaagt aaagcgtgac gcaatcatga tgaaacgaaa aaaacggctt
     1501 tttaaataaa cgaagtgtaa caaagaaaag gttgtgaaag agacgttcag ccttgagcga
     1561 tgacaaaata tctcacaaac attgcgacaa gtaaccgcag gagcaataag aagtaattat
     1621 gagtattaaa aaaagttttg taaaaatagc ttctcacatt tttacataaa attgacttaa
     1681 tactgagaaa ctgacttttg aacaccctct atctgtaaaa aaggggctgc gacatgattt
     1741 ttgtcacggc ccctcttctt tattcaaatt ttttccaaaa cgttcgttga taataaacga
     1801 tccatagagc ggaaagtatt agaactaaac cgatcacagg aatattcact aaccaaaatg
     1861 gataaaacaa agccaacatt ccgtataaga cgataagaat aatactgatg atcaatgaac
     1921 ccatcatagt tgcgactaaa cccaaacttc ctgaacctct gttaaataat tggttaatat
     1981 tggtccagtt cagcagcagc aaccgataat ctctagcgaa ataacgtagt gacaataagt
     2041 agcagccgaa taaagaacca aatagcaagc tagggataaa aaacaaaggc agctgaaaag
     2101 acagtccgct taataatgcg atcccaccag ttaaaaaact ctgcaatatc catccgaatc
     2161 tgaacttttc ttttaaatat tggttcatag agattggcaa agaacggata aacagaaaat
     2221 tttcttgatc caaagaaata agattgctga taaaagaagt ctgattaact gttaagaaag
     2281 ccaatgcgat ccctaccagg aaaacaacac cgattaactt attatctaaa tcagttaatt
     2341 ttatttcgcc tcctagggca aaagcaagaa taaaaatgat tggagttaac aaggaagaag
     2401 aaagaacttg cataatcaaa ttaggttctt ttactaattg agcattgtaa ctaaataaaa
     2461 gctgatgcac attttggttc tttttatggg ttctttttat tttgaactca ctgtttgaat
     2521 cagtcagttg ctctgtaagc tttggtacta tcagctgttt tacagcaagg aaaaatccca
     2581 gagccgtgat agctagtatt ccccaaccca ataaaccttg ttgagaaagt ggctgactgg
     2641 cagcatcata gaatggcaat aatatcgaaa tcggatgccg atctgttaac tcattttcaa
     2701 tagtccccgt ttgatgattc atccacatga ttccagcaaa tgcgatcagc atcgagctgc
     2761 caagcaagat actagaaact gtttttctat gttttttaaa cactgctgtt cgagccaaac
     2821 caaaaacaat caaactagcc agacaaaaaa gaataatcaa aaatagagaa aacaaaagga
     2881 ttgagataat gatagtaagc aaaatcccta gatcagctcg ccatccggtt aaaataaaca
     2941 tgactagaag cggaaaaaca aaaggaatga tggtcaaagc aactaccaaa atctttgctg
     3001 aaaaaacctg ttcttgtcca aaaggcagag gcaaaaatac ttgcaggtca tttccttcaa
     3061 aaaagatatt ataaatgact gatatacttt gagttaaacc taacatacca aaaagtgcca
     3121 cgtaataagt aaagaaacct ggcaatttaa caaagttcat agcaaacata gcaaatccgt
     3181 aaatcagtaa aaaaatcagc cctgaaaaaa gattttgaga caaaagatag atagttaatc
     3241 gctttccctt ttttcctttc tctcttgctt tattcgtgag ctgtggatta acatataaaa
     3301 gattaactcg aattaattct aataattgtc gcttatacat acggtccatc tcctgtcgct
     3361 tgccttccag ccattttcaa ataaatcttt tctagcgttt catcaggtga gccattcaat
     3421 aattcactaa ctgccccctg ataaatcaat tcacctttct tcaaaatagc cagtttatcg
     3481 cataactgtt gtgcggtatc caatacatgt gtagaaaaaa tgactgtttt tccttttgcg
     3541 gcatgagcct tcatcatttc tttcaaatca aatgctgctt gaggatcaag tccttgcaaa
     3601 ggttcatcca atacccaaat atctggatct ggcagcaacg tgccgatcaa aatcgttttt
     3661 tgacgcattc catgtgaaaa acttgcgatt gtttcatttt gatgactata catatcaaaa
     3721 agcgctgtca attctgctaa acgcttttct tttttttgtg tgtctaattc ataggcagct
     3781 gaaatcaagt cccaatattc tccagccgtc aattgtaaaa aaatatctgg ggaatcaggc
     3841 acataaccga ttttttgttt gatactcaat cgattttccg tcaatttcat tccatctact
     3901 aaaatcgttc ccgatgatgg ttcaataata ctaactaaac ttttaatcgt agttgatttt
     3961 ccggcaccat tatgtcctaa aaatccaaaa atttcccctt gtcttatcga gagagacaag
     4021 tcttttaatg cttgtttttt cccgtaactt ttacttacat tgatgaattc aatcatttct
     4081 gtactccttt tgtttgttat tactttttat tgtacccttc gttattggtg gagtaaatgt
     4141 caaaaagagt gcataacaaa aaaggataga aacgactagg ctgctacttg ggttgttctc
     4201 ttctacagga aaacactctt ctaacaggta agatatgagt ctttgttttc tttaaaatga
     4261 ttcattttct atcttttctt gcataaaatt ctttagttta tcaatacttc tctttcccat
     4321 ctctagttgt tctccatttg caaatgttac tatttcgttt actctgctaa tagatgtaat
     4381 gttttcagta ttaatgatat aagcttttaa atctttacat aaatactcat attcgagtga
     4441 ttttttatat tttttaattg tgccgtttac gagaatctct tcatctttca atactaggcc
     4501 tattttattt ttataacctg gcatgctcat tagataaagg atttcatcgt aatggatata
     4561 gtgatcgcta ttaccataac ggacagttgt ccaccttttt ttcgtagcat gttgaacagg
     4621 atagttttct ttttctttca ataagtaact ttggatgctt tttttatttt tttcataagt
     4681 gaattctttt atcaaataac cactaggcgt aatctttcga ttaataacag ttattgcttt
     4741 attcggatga gacgttagat aaacaatttt acacttttca ttcttttctc taagcatttc
     4801 gcctagttcg ataccgctat aataggcgtt caaatcgatg tctaggaaaa aaatatctga
     4861 ggaatagatt tcattatttt taatgaattc aacaaattgt ataacgttca atccctgaac
     4921 gatctcaaat ttccaaggac ttaataaaat tctggattct tccaaaattt ctactagcat
     4981 tttttcaaaa accaaactat catccaaaat aaatatacgc gttaccataa aaagtacctc
     5041 cactcgatat tttactatca aaatcatttc atacaacttt tcaacataca tgttaggaat
     5101 aaattgtaat tctcttcaca atcttttcca acacctttga aaccgatatc aaatactttc
     5161 aggatgctgc ttctaaaata aatatcaaac aaatgaatac atcatgcaaa aaaagccaga
     5221 caaccgacgt ccttgcgtgt cagctgtctg agctatgttt atttattttg ttcttttaaa
     5281 aagaacgata cacatactga tttttcggag cagagacttt ctcagcgttt gtaacaacta
     5341 cagatgggat ttcacgcttg aatggttgat aatagtctga ttggatggtt ccttctattt
     5401 tataccattc atcattttta accgttactc cttccggcat atgcgtcaac aaaccaaaga
     5461 ctcctgaatc tgccacacaa tggataatgc cgaatcggaa caagaagata tctgtattgc
     5521 tatcgttcgg tgtttggtaa acaaatcctt catagctgat ttttttgcca atgaattcgc
     5581 tagggtaatt ataaattagc tccataactt ctaaatagtt ttcatctgtg atcttgatag
     5641 tttgcttatc cccatacttt tctttcaact cattcatttg gtctaagtag tctgatttgt
     5701 tgaaataaat actggtatcc ggctttaagt attgtgtctg catctccgga tcccctactg
     5761 attctttact tactgggaaa ttaaatcctt tggcttctac gatcgtagtg tcgagactaa
     5821 ctgttggaaa caacgtcccc acgattactg gcaaagctag cagtacataa gcgattccac
     5881 gttgataagg ttttgacaac ccatggtcat gatcgtgctc gtgtgccttt tcttctgctt
     5941 ttccgttttt gacccacaaa tacagctgaa ctaaagccaa aagagcagat aaaatcattg
     6001 aaagaatagc taagtagcga taatggacat tgatatattg atcgagtttg ccagaaactt
     6061 gcaagtacat catcaattcg aaatacccag ataaaatcaa aaaccgcagc atattttccc
     6121 tcctaaataa ataatgtgaa tccaataacg ctaagggcaa tcagtccgat cagcgataaa
     6181 ataaagcgtc ctgtaaaata tcgtttcatc atcagtagat ttttgatatc gaccattggg
     6241 ccaaagacca aaaaggcagc tacaggtgct gtgccgaaca gacttaacaa agaagatcct
     6301 ataaacgcat ctgcttctga gcaaagagac atcgtaaagg ccaataacag catgatgata
     6361 atggctaaaa tctttgttga gccgagcgtt agcataattc tagttggtaa ataagtctgc
     6421 atcgcagctg cgattaatcc gccaatgatc aaataacgac ctgtatcaaa aaattcgtcg
     6481 attgaatgtg tcaacacatg cccagtttga tggatcagct gattttttgg ttcattcttc
     6541 tcactttgtt cttcttcatg gacatgatga tggtgatttt ctgctacttt agacgtgatt
     6601 ttttctttca atacagcgtc tttttgaaaa taggcaagcc agacaccaat cacaagggca
     6661 acaaaaaagc tgcctatcac ccgccagctt gccatctgcc aagaattgcc aaatgcgata
     6721 aaagtggaaa aaatcacgat tggattgatg atcggagcag tcagcataaa agcaaacgct
     6781 gtgtgaactg gtacgccttt tttaacaaat tgatgaacga tcggtacgat gccgcattca
     6841 catgaaggaa agaaaaatcc taagatcgaa ccagtaaaaa tagagaagat acgattttca
     6901 ggaagccatc tcttcacacg ttctggggtc aaaaatactt gtaaggcgcc tgagataaga
     6961 cagcctaata atacgaaagg taatgcttca ataacgatcg ataagaaaat cgtactcatc
     7021 tgcaaaaccg aatttggaat aaaagaaaac atgagtttcc tcctttttat ttctagttca
     7081 ttcgctttgc aaatatacgc ttttattcaa aaaaaacaag ttgctctgta aatttttaca
     7141 caactcttat tttttaccaa agcacttcaa ttgacttttt ctgttattcc cattaaatta
     7201 aggtctcata catatcactt ctatcataaa agggagaata aaaatgaaac aaaccactaa
     7261 cttgactgaa gttcaagaca tactgcaaca gtcatccgtt gccgtcattt atctttcgat
     7321 gcctaattgc agtgtttgtc acgctgttcg tccacgacta gaggaacttc tcacacatta
     7381 tgatattccg gcacttcatt tggatgcatt tgaaacacca gaagtagcta gtaaatttga
     7441 agtgctgact gcgccagttg tcctgatttt tcatcaagga aaagaagtct ttcggcaagc
     7501 acggttcatc gactttaaaa aaatccgtta tctactagat gaactgacag cagaagatga
     7561 ttccctttct tatgaagaaa tcttccgaac aaaataatcg ttgtccaaac agaaaaaagc
     7621 gattgaacaa gtgaaagttt tgccttcatt tgttcaatcg ctttttattt ttattctgct
     7681 gtattttcgg aatgcgcatt tgcgtcagaa tgcaagaaat agatcaaggt ttgcaattca
     7741 ttcgtcaaat caacattttg gatcaataca tctgacggag cattcaatcg agcagcggta
     7801 aagttcaaga ttcctttgat tcctgcttcc gttaactgat tgactacttc ttgtgctctt
     7861 tcagcgggta tcgttaaaat tgctacttcg atttgttgga tcttgatttg ttctgacata
     7921 tcttccattg gataaacggg gataccgtct acgatgcgcc caacgatttc ctcattcaca
     7981 tcaaaagcac aactgatacg tatgctatta ctttgatgga atttgtattt tagtaaagca
     8041 cttcccaagt tccctacacc gatcagcgca acatttgtca attgatcgtc gtttaatgtc
     8101 tttgcaaaaa agtgcataag atcttctaca tcataaccat acccacgttt tcctaattcg
     8161 ccaaaataag aaaaatctcg acggatcgtt gcactatcta cttgaacggc ttcacttaat
     8221 tcagttgaag acactttttt ctttccagca ttatttaaaa tccttaaata gcgataatac
     8281 aaaggcagtc ttttagctgt cgcctttgga ataatatgat ctttcacgaa atgacctcca
     8341 cgtattgtct aattacatct ttgatttctc catcatagca gtatttatag gaaaaaatca
     8401 attccattgc tcagatattg tgaaatttta ttctttattc atttatttat catttctgca
     8461 aatctcgcag tataatggaa tttatggtag actactaatt gaaaaatagt tttttatttg
     8521 aggagaattc acaaagtaga aaagaggatt ccatgatatt attacaagcc aatcagattg
     8581 ctcgccattt tggtgcagaa gtcttatttg aaaacataca tttagaaatc agtacaggcg
     8641 ctcgtgtcgc tttagttggc agaaatggcg ctggaaaatc gactttatta aagatcattg
     8701 ctggaattga agcacctgat gaaggaaaga tcgctaaaaa taaaacagcg actctcggat
     8761 acctcgctca ggacacagga cttgattcca atgaaacagt ttggaacgag atgctgaaag
     8821 cttttgaaca agttagacaa atggaagagc ggatgcgcga gctagagatt ttcattagcg
     8881 aaggtacgcc agacacacct gcttatagtt cttatttaaa agaatacgac cggctgcaac
     8941 acgatttttc agatcgaaac ggctatggtt atgaaaatga aattcgttcc gtattgcatg
     9001 ggtttaaatt cgattcttca ttttatgatc agaaaatcga tactttatca ggcggacagc
     9061 gcacacgttt agctttagct cgcatgcttt tgcaacaccc tgacattttg attctagacg
     9121 aaccaacgaa ccatttagat atcgagacac tttcctggct tgagaattat ctgcaaagct
     9181 atactggtgc attattgatt gtttcccatg accgttattt tttagataaa gtagtcaatg
     9241 aagtctatga attaagcaga agaaaaatgc gccattacaa aggtaactat tcgaaatatc
     9301 tagatttgaa agcagaacag ttagcaagtg aatggaaggc ttatgaaaag cagcaaacag
     9361 aaatccataa attagaagac ttcgttgctc ggaaccttgt tcgcgcctct accacaaaac
     9421 gagcgcaaag ccgcagaaag acattagaaa aaatggaccg gctggatcgt cctcaagggg
     9481 atgaaaaaca agcaaacttt ctattcagta tagaaaagcc ttctggaaat gtagtcttgc
     9541 aaatcgaaga tgccgctatc ggatatgata atgtcacctt atctgaacca atcaatctag
     9601 atatccgtcg tcaagaagcg attgctctag taggtccgaa tggtatcggg aaatcgacgt
     9661 tgctgaaatc aatcattcac cagatccctt ttatccgtgg aaaagaaacg ctagggacaa
     9721 atgtcaccat cggctattac gatcaaggtc aagctgatct gcatacaaac aagacgatcc
     9781 tagcagaatt gtgggatgag catcctacca ctccagaaaa agagatccga agtgctttag
     9841 gtgctttctt attttctggc gaagatgtag aaaaaccaat ccctctgcta agtggcggtg
     9901 aaaaagcacg ggttgcttta gccaagttag cgatgaacaa agaaaatttt ttgattttgg
     9961 atgaaccaac aaaccattta gatattgaca acaaagaagt attagaaaat gcattgatcg
    10021 attatgacgg cacactgctt tttgtttccc atgaccgata tttcatcaat cggatcgcga
    10081 caaaagtcgt cgaactctct gaaaatggca gcaaagtcta tctaggagac tatgattatt
    10141 atttagagaa aaagcaggaa gaagaagaaa tcgccgaatt gttggcacaa gatgagccag
    10201 aatctgctcc tgctcccacg aacaaatctg attattacca aagcaaagaa cagcaaaagc
    10261 ttatccgtag ccttcaacga aaaattactc aggttgaaga agaaatggcg cggatcgatg
    10321 aatggatcga tcagttggaa caagaaatgg tcgcgccaga aaacctggaa gatcacgtaa
    10381 aactcaatga gctgaaccaa gatttagaag ctgctagaca agatcaagaa acaaaattga
    10441 cggaatggga agaactaagc ttggaattag aagaatatga aaattaacga aaaaaccaaa
    10501 acagaaacca ttcatgctgt tttggttttt ctttttttat tttatcaaac aaaaattcca
    10561 tatttacctt tttcaccaat aagaagacca attactagtc attctcacta aagcttctaa
    10621 ataatagtaa tcaccccaaa tacaaggtgt atttacccca ttatttttgg gcttattata
    10681 aacagactgt gttaaaaagc catcttcttc ttccgttgta gcatatcttt ctttcaattg
    10741 tcgcataatt ttccaaacag cagactggta taagttcttt tctgttttgt ctaatagacc
    10801 tgattctatt aattctaata atccacaaac catgatcacc atactagatg tatctttttc
    10861 ttctctgcta gaatcatcaa agatcaaatc ccaataacaa atggtatctt ctggcaaccg
    10921 attcataaaa taattggcta atattttgct taaatcaaaa tatttttgtt ctttcaaata
    10981 acgataactt aatgcaaaac cgtaaatccc ccatgcttgg cctctagccc aacaggaatt
    11041 atcactataa ccttgatgcg tacttccata tcttggtttt ccatctttga catccatata
    11101 aaatgtatga taagttgaac catcttctct tactaaataa tctgctgcat tttgaatatg
    11161 agcctgagca gcttttttat atttttgatt tccagtttct tcactcgccc aatacaataa
    11221 tggaagattc atgcaacaat ccatgatcat tctaccttgt tgtgaaggat cactaggatc
    11281 tccccaagct tgtataactc ctgccttctc ccaaaaacgc tgaagtaatg tttctgcagc
    11341 atcaattgaa gcttccttca tttttcgatt tcccgttatt ttataatctg ctacggttga
    11401 taaagaatac aaaaaaccaa gatcgtgatg attagtgata atttgacagt caatcctgtg
    11461 gataaaatct atatctgctt tttcagctgt ttgttgaaat aaaggatcac ccgtcttctc
    11521 aaataacaac caattaattc ctgtccaaaa tccttctgtc caaccaatat tgtctcttaa
    11581 tggataacgt ccttttatag gacactcgct tgggaattga tactgactgt tactgatacg
    11641 tttgattaac ctctccgtgt gtctgcgaca acgctcgatc tcattattcc attctttttc
    11701 cattaattct ttataacatt catttatcca tttttctgga tgtattatct cttcaaacat
    11761 cactttatca agcatttccc caccagcctt tagcataatt ttagtctttt tttattgttt
    11821 ttattttgga tagttagccc ggttgtccca tcaaaatcaa ttttgggacg attgttccat
    11881 acttctaaac tataatcccg atcttgatga gcaaccactc ctgttccgag ccaatgggtt
    11941 ccttttccaa atgtaccctc taatccagga ataaaagtgt tttcccatgt catcaagttt
    12001 gtattgggca aacccataat agctgtggat tttcgtttaa tagtatcgtc acttaaacag
    12061 ataatacctg aaaagccagc ttcactcacc ctatagtttt ccttgtcttc ttctatgatt
    12121 attccttctt tctcaggaga acattcaata gcaaaccctg tctcatatag aacatattct
    12181 gtatctaatt ctactttgtg tacgcgaatc tgccagcctt caaaaggaat aagccaagtt
    12241 ctgatttttg cctgtaaagt aggttcccat gtagaggcaa gtacctcttt cttacataca
    12301 atgtctcgta cattatttcg aacgatgaac tgtccatagg ttttgatgct ctcattctta
    12361 aaactggtcg tgatatatgt gtccgctcgc tgaatcccta gcattgaatc cattgccatt
    12421 tctgctttca attgattcgc tcgcggtatc gaaaaaccaa attgactaga ataagcaaat
    12481 tttaaatatt tttcattggt caatgtatgg aaatttggcc ctaattgtcc cgcattcaaa
    12541 aaaaatgtat gtccattatc atgttgtatg agcaaattgg cttctgaaag aagtttagtt
    12601 ccttcacgaa taggcatagg catttcttta gccgtccaat actcatggtc ttcaggtagc
    12661 gccaacaata aaaaaatctt attcagccat aatggagata acgtactatt atagggttca
    12721 gtcaatgtca attgagggta ggtataacca atattcagga tacctttttc atcaaatatt
    12781 tctttttgca tccaccatct tatattccga ttgactaacc ccttgatttc acctaattta
    12841 aaaggcaaaa gatcggccca caacatagct gaccaaaagc ttgaaactgc aaaacgataa
    12901 atcatgcttc gaccaaaagg cacatttgca ccatcttccg caaaaaaatg aatatagtct
    12961 ctagaaaaat caatagctcg gttcttatat atctggctaa tgggcttttc tggttctagt
    13021 acgctataaa tcaaaccata ataatgaaat gcaaaaggat tataataatc ttggcgacct
    13081 cttgaactgt ctcgatacca gccattttga agataacaat tttctatatt atcaaatgct
    13141 tctttcagtg ctgattcttt aactggataa ccaaattttt ggataattgt tccgacaatt
    13201 atcttaaaaa attgccaatt accatctgca cattcatatt caaaaatttg cgagaaccaa
    13261 tgaataatct ggcttttttc tcctgtatct aattttttcc ataataattg ttcatgatga
    13321 tataacgcta ataacattgc gggcatttct accatacgct ggtctctttc atgtatttct
    13381 ccccaataat cgggatggga aggatctgtt ccagctttta ttcctgatag aattaatttc
    13441 aaccatcgtt catctaattg gtcaattgcg ggccccgctc cccataaaac ccgagagaat
    13501 ccttccattc ctgcaatttt atttccatat cctacccctt gatgtatata atcaatttgt
    13561 gcgccacttg ctcgataaaa cggctggagt ggttgattta tctttttaaa taagtcaaag
    13621 taatcctgtc tggtccttaa tagtggtact ggatgtttca ttgtattttc tcctcttttt
    13681 actaccaaat agctaattcc gactgttcta atcgaagtaa gccttcaata aaaaaataat
    13741 cagaatagat cagtggcaca ttataagtca gttctctatg gtatgcttct gctccgcctc
    13801 caatgatccc atcttcatcc atattccaat ttgcatagtg cttttctgtc ttttggagta
    13861 tattgatagc agcttcatga tacatttttg atcgtacaga ggaaacatat cctgctaatt
    13921 ccagaagacc gcatgcagcg catactccag cactggtatc aatattcgaa tcttttacag
    13981 caaaatcaat gagaggaata taaccagttt tttcgatttt ctgaataaaa tttactgcta
    14041 tcttttctgc tatagctaga tagtacggat tttttgtgta acgataactt aacgcgaatc
    14101 catataaagc ccatgcattt cctctgctcc aacaagaatt ctcattgtat ccttgcccgc
    14161 caagtaaccg aatatatttc cctgattctg catcaaattc tgcgatatgc ccggttgatc
    14221 catctttcct cactagatac ttagctgttg tatctgcatg ctgccgagca attttagaga
    14281 acctcggatc accagtttcc ctcgaagccc aatacagtaa tggtaaattc atcatactat
    14341 caataatcat ccaaccaggt ttatggtcat tccatgctac taaatagttc cctgaaatat
    14401 tatagcgtcc agcaagtacc gtagctgcat gtaaccctag ttgtcgacta tgtttgtttt
    14461 gagactgttg ataatgtgca atagccgatg gtaagaataa aaaacctaca tcatgatgaa
    14521 cgtctgaaaa atggtacagg gcagattcta ataattgttc ttgttgtatt gctgcctctt
    14581 tgaacttttc tcgttttgtc ttgtgaaaga gctgccataa aataccactc caaaaaccat
    14641 tggtccacca gtcgattcct ctgtccttta ttacgtctgt atattttccg ttgtaagaaa
    14701 tatatggaat tttattcccc attctgtcta attccgcatc tatcttttca gaagctacat
    14761 tcaaaatttt tcttgaccat tccttcgtct gttcattcat ggatatcccg ccattctttt
    14821 aatttttatg aatgtggagt tgctcctaga acgaactaga tggttaacat tcgttatttt
    14881 ttttacttat ttgcagctcc aatgtcattc tccttaaatt tagactgcgc ccacacataa
    14941 atcaagacca ttgggatcac tgaagttgct aaaattagca taattgcgcc ataattaatt
    15001 ggctcacctg tactagaaac cgtcatattt ttgataatta agggaaccgt atatttatct
    15061 ttatctgaaa gcatgactaa tggaaataga tattgattcc aacttcccat aaagataagt
    15121 aaaccactag taataatcgt aggtgataaa atcggtacag cgattttcgt aaaaatcacc
    15181 cactcattgg caccatcgat tctggcagcc tccatcagtg ctgtaggaaa ttgactgcta
    15241 tattgtcgca tcataaacac tgaagttgta ctggcaatcg aagggatgat cacacctgca
    15301 taagtattcg ataatcctaa acgactcata atgtaaaata aaggaatcaa tgtggtaaaa
    15361 aaaggcacca tcctagatag cataactgta ttgaacaaca gtttattccc cataaaacga
    15421 tactttgcta atgcataacc cgccattgta gaactggctg tagctgaaac tgtactgatt
    15481 acagcaataa caatcgaatt ccaaataatc aagggataat tatatctcgc catgatggct
    15541 tgccagtttt ttaatagcat atccccaaac ttgaaattga ttccgccact taaaatttct
    15601 tgattacttt gcgttgaagc tacaatcata tataaatatg gcatcaaaaa tattaaggca
    15661 ccaaacacaa taatgacgta cgtaaaaatt ctgctttgta tcttttgttt agtcattttt
    15721 atcacctccg aatttaaatt gaatagccga taatataaca gatacaaaca ccatcgtcca
    15781 ggcaatagca gaggcttttc caaaatcaac catttcaaaa cttgttttat ataaattgac
    15841 tcctacgacc aaagctgaat tttctggtcc attggaagtc ccaaagaata tatttgggat
    15901 attgaaatta cccaagccat tgatagtggc taaaactgtg gccattaaaa tgattggctt
    15961 tagcatgggg atcgttaata aaaagaattg ccgaaatttc gatgctccat ctatttcagc
    16021 actttcgtat atctctttag aaatcccttg aagaccagct aaaaataaaa tagtgttata
    16081 tcctacacta agccaaatca tcgtgatgat cattgatatt cgtatccaaa aaggatttgt
    16141 taaccactgt acaccgttaa ttcctagcgc attcaaaaat ccattgaata acccagtcgg
    16201 attgaaaaaa gtcatgaata cggaagctac agccaccgtt gatgtaatgg ttggaatata
    16261 atagacagtt ctaaaaaatc cttttccttt taactgtgct gaattcaata aaacagcaaa
    16321 gtaaatagct aatagcatcg ttattggaat tgatcctgcc atgataatca aaacattcgt
    16381 gtaagattta ataaacaatg gatctcggaa gatagctaga taattgtcaa atccaacaaa
    16441 ttcaaatgag tagccaattt tcataaaact aaaagcaaac gagataatga tgggaatcaa
    16501 aatcgtcaca ataaagaaaa taacaaaagg tgtgaccaat ccataagcac tttttttatg
    16561 agacgtatct gttgcttttt tcttgctgtt ctttattgtc ttgcttggta acgaagattg
    16621 catcatcgtt aagtcctcct aatatttttt aatcaccagt taattttacg ccgtacttat
    16681 gagcaaaatt ttctgcttct tttagatatg aattttcaaa atcacctgtt tgccaatagt
    16741 tatatgcagc ggtagtcaaa taagacgtag catctgaata agtggctggt aaaacagtat
    16801 ttgatgtatt ttcattgatt ttattcaata gttcatagta tttttgtcct ccttagtagt
    16861 caaacttttt ctcagctgct tctgtttgat aggccaatga gttagatgta gccacgccca
    16921 gctccagtgc gttggctaag cattcttcat ctgtcagcat aaatgtaaga aattgttgtg
    16981 ccacttgtgg atttttggca tctttaggga cataaaaact tgatcctcct gatactgcac
    17041 cttttcctgg attttctttt gaaaaaggaa tcagtggagc catgcgccat ttgtcagatg
    17101 catcaggaaa attcaaagac atattcgtag cataccagcc gccagcaaca aacattgcag
    17161 attcttgaaa tgctttctca gcatcttgtg caccgtagaa atgtacaata tctgagtcga
    17221 tcatttcttt gattaacttt gcagcatgtt tcgcttcttt agtattcaga cggatatttc
    17281 cttcttcatc aagcaaagat tgattctgct gggctagcat gttgataaac aattcttggt
    17341 ccccagattc tggcaaggca attccaaaac taccagtttt ttcttttaat gcttttgcca
    17401 cctggatata ttcttcccaa gaactgattt cttcaaaatc tacacctatt tcagcaaatt
    17461 tttttggttg ataataacaa agtgctgggg taaaatcact agcccaagga ataatcgttt
    17521 cattactcgt ttgttgacgc aaaatattca atttttgatc ataaaactga tcgccatatt
    17581 tgtcataaaa tccatattca tcagcactat aaaaagcatc ttgaaatttt tccagcacac
    17641 ctgctgctga agtgtctgcc atggagataa tatccggcat atcttcgtgt gttaccaatt
    17701 ttggggtgat catttgtact gttgctgttc ccgaagctac attcacaatt tttaagtcaa
    17761 tatttgctcc agttttgcgg ttatagattt ttgcagcatt ttcaacatag tttgtcagtt
    17821 tgccgtttgc gacatatact tctaatgtag tggctttttt tatcattgga tctgcgatgt
    17881 ttgctgttac ttcttcgtta gtttttccac atgcacttaa gctgaataat agggtaatgt
    17941 ttagcaaagc tatcaccaat ttgtatcttt ttttcatgtc tatcccttct ttcttttcaa
    18001 gttcttatat aaaacgcttt cacttatgta tattaacgga attagtaaca caaatattta
    18061 tataaaaaaa tattttactt gtcgttttgg cttttc
//
LOCUS       DACEPW010000043        17557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEPW010000043 DACEPW010000000
VERSION     DACEPW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.044"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            369..998
                     /locus_tag="IW819_001148"
     CDS             369..998
                     /locus_tag="IW819_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244264.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(1122..2231)
                     /gene="ugpC"
                     /locus_tag="IW819_001149"
     CDS             complement(1122..2231)
                     /gene="ugpC"
                     /locus_tag="IW819_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1244265.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2440..2862
                     /gene="mgsA"
                     /locus_tag="IW819_001150"
     CDS             2440..2862
                     /gene="mgsA"
                     /locus_tag="IW819_001150"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1244266.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            3185..3721
                     /locus_tag="IW819_001151"
     CDS             3185..3721
                     /locus_tag="IW819_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1244267.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(3761..5524)
                     /locus_tag="IW819_001152"
     CDS             complement(3761..5524)
                     /locus_tag="IW819_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244268.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(5527..7242)
                     /locus_tag="IW819_001153"
     CDS             complement(5527..7242)
                     /locus_tag="IW819_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244269.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(8021..8245)
                     /locus_tag="IW819_001154"
     CDS             complement(8021..8245)
                     /locus_tag="IW819_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244270.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            8445..11213
                     /locus_tag="IW819_001155"
     CDS             8445..11213
                     /locus_tag="IW819_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1244271.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            11294..11713
                     /locus_tag="IW819_001156"
     CDS             11294..11713
                     /locus_tag="IW819_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1244272.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            11725..12195
                     /locus_tag="IW819_001157"
     CDS             11725..12195
                     /locus_tag="IW819_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1244273.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            12212..12991
                     /locus_tag="IW819_001158"
     CDS             12212..12991
                     /locus_tag="IW819_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1244274.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            12978..13799
                     /locus_tag="IW819_001159"
     CDS             12978..13799
                     /locus_tag="IW819_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1244275.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            13816..14865
                     /locus_tag="IW819_001160"
     CDS             13816..14865
                     /locus_tag="IW819_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1244276.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            14878..15882
                     /locus_tag="IW819_001161"
     CDS             14878..15882
                     /locus_tag="IW819_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1244277.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            16515..17534
                     /locus_tag="IW819_001162"
     CDS             16515..17534
                     /locus_tag="IW819_001162"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1244278.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         5626 a         3082 c         3568 g         5281 t
ORIGIN      
        1 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
       61 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
      121 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
      181 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
      241 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
      301 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
      361 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
      421 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
      481 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
      541 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
      601 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
      661 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
      721 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
      781 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
      841 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
      901 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
      961 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
     1021 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
     1081 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
     1141 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
     1201 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
     1261 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
     1321 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
     1381 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
     1441 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
     1501 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
     1561 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
     1621 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
     1681 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
     1741 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
     1801 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
     1861 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
     1921 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
     1981 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
     2041 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
     2101 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
     2161 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
     2221 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
     2281 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
     2341 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
     2401 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
     2461 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
     2521 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
     2581 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
     2641 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
     2701 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
     2761 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
     2821 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
     2881 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
     2941 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
     3001 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
     3061 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
     3121 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
     3181 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
     3241 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
     3301 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
     3361 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
     3421 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
     3481 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
     3541 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
     3601 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
     3661 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
     3721 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
     3781 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
     3841 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
     3901 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
     3961 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
     4021 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
     4081 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
     4141 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
     4201 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
     4261 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
     4321 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
     4381 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
     4441 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
     4501 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
     4561 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
     4621 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
     4681 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
     4741 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
     4801 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
     4861 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
     4921 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
     4981 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
     5041 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
     5101 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
     5161 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
     5221 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
     5281 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
     5341 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
     5401 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
     5461 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
     5521 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
     5581 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
     5641 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
     5701 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
     5761 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
     5821 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
     5881 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
     5941 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
     6001 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
     6061 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
     6121 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
     6181 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
     6241 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
     6301 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
     6361 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
     6421 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
     6481 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
     6541 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
     6601 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
     6661 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
     6721 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
     6781 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
     6841 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
     6901 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
     6961 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
     7021 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
     7081 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
     7141 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
     7201 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
     7261 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
     7321 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
     7381 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
     7441 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
     7501 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
     7561 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
     7621 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
     7681 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
     7741 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
     7801 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
     7861 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
     7921 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
     7981 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
     8041 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
     8101 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
     8161 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
     8221 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
     8281 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
     8341 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
     8401 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
     8461 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
     8521 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
     8581 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
     8641 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
     8701 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
     8761 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
     8821 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
     8881 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
     8941 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
     9001 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
     9061 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
     9121 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
     9181 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
     9241 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
     9301 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
     9361 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
     9421 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
     9481 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
     9541 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
     9601 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
     9661 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
     9721 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
     9781 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
     9841 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
     9901 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
     9961 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    10021 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    10081 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    10141 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    10201 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    10261 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    10321 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    10381 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    10441 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    10501 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    10561 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    10621 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    10681 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    10741 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    10801 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    10861 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    10921 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    10981 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    11041 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    11101 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    11161 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    11221 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    11281 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    11341 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    11401 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    11461 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    11521 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    11581 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    11641 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    11701 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    11761 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    11821 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    11881 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    11941 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    12001 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    12061 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    12121 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    12181 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    12241 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    12301 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    12361 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    12421 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    12481 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    12541 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    12601 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    12661 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    12721 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    12781 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    12841 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    12901 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    12961 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    13021 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    13081 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    13141 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    13201 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    13261 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    13321 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    13381 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    13441 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    13501 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    13561 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    13621 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    13681 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    13741 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    13801 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    13861 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    13921 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    13981 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    14041 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    14101 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    14161 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    14221 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    14281 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    14341 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    14401 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    14461 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    14521 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    14581 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    14641 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    14701 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    14761 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    14821 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    14881 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    14941 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    15001 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    15061 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    15121 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    15181 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    15241 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    15301 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    15361 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    15421 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    15481 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    15541 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    15601 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    15661 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    15721 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    15781 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    15841 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    15901 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    15961 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    16021 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    16081 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    16141 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    16201 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    16261 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    16321 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    16381 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    16441 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    16501 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    16561 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    16621 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    16681 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    16741 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    16801 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    16861 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    16921 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    16981 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    17041 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    17101 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    17161 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    17221 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    17281 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    17341 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    17401 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    17461 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    17521 tttctcttcg ttaaatagat gatcaatagt taatcgt
//
LOCUS       DACEPW010000044        17417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEPW010000044 DACEPW010000000
VERSION     DACEPW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.045"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(39..1240)
                     /locus_tag="IW819_001163"
                     /pseudo
     CDS             complement(39..1240)
                     /locus_tag="IW819_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1357..1872
                     /locus_tag="IW819_001164"
     CDS             1357..1872
                     /locus_tag="IW819_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1244279.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2071..2517)
                     /locus_tag="IW819_001165"
     CDS             complement(2071..2517)
                     /locus_tag="IW819_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1244280.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2564..4042)
                     /gene="msr"
                     /locus_tag="IW819_001166"
     CDS             complement(2564..4042)
                     /gene="msr"
                     /locus_tag="IW819_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1244281.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4396..5025)
                     /locus_tag="IW819_001167"
     CDS             complement(4396..5025)
                     /locus_tag="IW819_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1244282.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5009..5473)
                     /locus_tag="IW819_001168"
     CDS             complement(5009..5473)
                     /locus_tag="IW819_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1244283.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5960..7228)
                     /locus_tag="IW819_001169"
     CDS             complement(5960..7228)
                     /locus_tag="IW819_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1244284.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7459..8196)
                     /locus_tag="IW819_001170"
     CDS             complement(7459..8196)
                     /locus_tag="IW819_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1244285.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8263..8832)
                     /locus_tag="IW819_001171"
     CDS             complement(8263..8832)
                     /locus_tag="IW819_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1244286.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8960..9832
                     /locus_tag="IW819_001172"
     CDS             8960..9832
                     /locus_tag="IW819_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1244287.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10174..11046
                     /locus_tag="IW819_001173"
     CDS             10174..11046
                     /locus_tag="IW819_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1244288.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11043..13001
                     /locus_tag="IW819_001174"
     CDS             11043..13001
                     /locus_tag="IW819_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1244289.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13252..14103)
                     /locus_tag="IW819_001175"
     CDS             complement(13252..14103)
                     /locus_tag="IW819_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1244290.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14226..14693
                     /locus_tag="IW819_001176"
     CDS             14226..14693
                     /locus_tag="IW819_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1244291.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14956..15438)
                     /locus_tag="IW819_001177"
     CDS             complement(14956..15438)
                     /locus_tag="IW819_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1244292.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFAKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15538..16401)
                     /locus_tag="IW819_001178"
     CDS             complement(15538..16401)
                     /locus_tag="IW819_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1244293.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            16999..>17417
                     /locus_tag="IW819_001179"
     CDS             16999..>17417
                     /locus_tag="IW819_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244294.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDK"
BASE COUNT         5224 a         3600 c         3086 g         5507 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt ttcaaaccga tccgactaga taagaaacct gtaaatagca
     1021 tcaccacacc agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag
     1081 cgtttaactg tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag
     1141 cgatcataca ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg
     1201 tttgttcttg tatatctatt tgattctcca atatttccat ccctttctct ctattagcat
     1261 catacatgat ttattttaca tgtaaatatg cacatgtaaa acgtggaaac tttactgtga
     1321 tataataaag aaaataagac gagaggtagg atttttatga atactttagc ctatatatta
     1381 ttatgcgtgc ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt
     1441 ttttgggaag cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt
     1501 tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat
     1561 gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa
     1621 gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga
     1681 ctgctattca ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg
     1741 aatgacctct cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga
     1801 attatgcttt gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag
     1861 ttttttgagt aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc
     1921 ttcaataaaa gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc
     1981 tatcgttcgg atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc
     2041 caaggccttt tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac
     2101 acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat
     2161 tttccaagca ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac
     2221 accgaataaa gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat
     2281 catttggacc ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac
     2341 accgatgatg aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa
     2401 caaacttgat aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac
     2461 catcaaaaaa cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct
     2521 gtaccactcc tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa
     2581 gtactttttt tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa
     2641 agtaagaatc atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta
     2701 aagcttcgat cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac
     2761 ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac
     2821 ataacggttt cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa
     2881 gcgattctga aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca
     2941 tttgataacc cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt
     3001 ctccttgttt gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa
     3061 tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac
     3121 ttctttccaa ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt
     3181 ttggtactgg aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt
     3241 ggagtcttct ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt
     3301 gtttagagga agacaaccga tccggtctga tttgttgctt tttcttttta ctggacactt
     3361 tttccaactt ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct
     3421 ctttcatgat cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga
     3481 gataggcact ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt
     3541 tcgatgctag ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat
     3601 aataacgaag ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct
     3661 cgtctagaag catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt
     3721 cttcccctcc gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg
     3781 ctaattccaa atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga
     3841 aggtgatctc tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga
     3901 gcaaagtcga tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga
     3961 cacgaagaga atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta
     4021 tatttactgc taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat
     4081 cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta
     4141 ctctgaacga aataactatg actaaatcac caaatctgtc atagctttca cctattctac
     4201 tgtcgttcga aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg
     4261 aatttattta gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc
     4321 cgacctcctt ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata
     4381 gtaactgaaa accttttatt cgaacgtagt gatcttttct ggtgctaaac gaacagactc
     4441 gtaaccttct tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt
     4501 atccaaacca aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc
     4561 atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt
     4621 gactacatcg ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa
     4681 ttgttgatct ctgacttctt ttggcatctt ccctgattcg taagcttggt tatagatttc
     4741 ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc
     4801 attttgtctc gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc
     4861 agcgaccaca aagcgccatg gttgcatatt gacagaagaa ggtgcagtcg tcgcttcttt
     4921 gatcatttcc agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga
     4981 tcttctacca gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt
     5041 tcgtttctgt tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt
     5101 gtttcatttc tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca
     5161 tttcacaatg ctcgattttc ttctgggctt tttctgttag cgctacatac acttcccggt
     5221 tgttctgagg atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac
     5281 gagtaactgc tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat
     5341 tttcttcttt cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt
     5401 caaagatctg gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct
     5461 cttctaaaga cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa
     5521 taatagaata aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc
     5581 aatagaaaag tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa
     5641 agcacaacgc taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg
     5701 cagtagaaaa ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg
     5761 aatattagaa attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg
     5821 tcacaacctt ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac
     5881 caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca
     5941 gctttttcac tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca
     6001 tcggtgttag ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt
     6061 catttggatt aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt
     6121 cataacgcca accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat
     6181 ggtctccact gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc
     6241 aagcaatccc ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca
     6301 atgtatataa atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag
     6361 ttggtgctgt atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac
     6421 cataattagg cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt
     6481 tcgtattgct tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct
     6541 tcaataccgt tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg
     6601 cattcaccgt aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc
     6661 cattatagct tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt
     6721 tcccccagcc actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg
     6781 cacttgccaa actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag
     6841 cagatgagcg agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt
     6901 gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct
     6961 ctggattgtt ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt
     7021 cagttgttgg ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac
     7081 tctctgtatc gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt
     7141 ctgtcgattc ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa
     7201 cgctaaataa taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc
     7261 taaataacag tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt
     7321 gtttcttttt gataaatttg ctttttctac ataaaaagac gtaaatgaag aatattcttc
     7381 tccattcacg ccttaaattg atggtcgttt gaataactat ttaacaagta attctctttg
     7441 gatattatcc gttatggatt atacttcctg tgtcgtcgga cggataatca tgttactgac
     7501 agacatccgg tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcggaggt
     7561 caatccccat tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt
     7621 ttgatataat tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg
     7681 ccgttgctct tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata
     7741 aacggcggaa tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc
     7801 agatttttgt ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat
     7861 gttgatatca agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg
     7921 catgattcct gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc
     7981 gataactgct tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc
     8041 ttctggtaaa gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag
     8101 tttagcccct ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc
     8161 catgatgaca atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa
     8221 atctcttgct tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac
     8281 aggtttgacg attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt
     8341 aacagttgca ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt
     8401 tgctcctgct ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct
     8461 tcgttctttt ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa
     8521 tactgaatct tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa
     8581 gacatttttc cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt
     8641 caccgtatca tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg
     8701 ttcatggtac ccagtattca gcgctgctaa aggctcttcg ttttcagctt ggatctgatg
     8761 gatcacttca aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat
     8821 gaggcttgtc attttgctcc ctccttttat ccttacttta taacggacgg cttatcatgt
     8881 ctaatactta taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt
     8941 taaaaacagg agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag
     9001 agaaaaaaat atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa
     9061 acaactgaaa gatttagaag aagaacttgg tgtgacactt ttcactcggg gctcccgaga
     9121 gatacagttg acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt
     9181 ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat
     9241 tggcggaggc gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa
     9301 ggcgcatcct gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa
     9361 actggatagc ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata
     9421 ccagtcattt cgactgccag cccaagatcg ttggggactt ttgatacatc gaaatcatcc
     9481 tttagcaaag caaaccgtca tccatccgca ggaactgaaa actgttcctt tgatcatttc
     9541 ccggcaaacg tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt
     9601 atcgatcgtc ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac
     9661 ttttggtgca ttagctctag atgggatcat caatacttat gggacggaaa aaatattcat
     9721 cccactcgat ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct
     9781 ttctcctgct gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt
     9841 tttttccttc gctttcagct ttttaacaac gaataagata agcaaaagat ccaacgatta
     9901 aataacataa gtttcttaat ttatttcttc cttttatttc tgataattcc agtaaattac
     9961 tataattata aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa
    10021 caataattcg tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg
    10081 tttccctagg aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa
    10141 cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa
    10201 aatgcagtat tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga
    10261 tttgacagaa aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg
    10321 gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta
    10381 tttgcagcag aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa
    10441 catgtattga tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt
    10501 atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt
    10561 attgctcaat gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac
    10621 gaagcaggat atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat
    10681 cgcagtatcc gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg
    10741 gaaacagata tccattcaga accattttca ctgaattatt caagactggt gaatcattta
    10801 cgactgcttc tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta
    10861 gaaatggtca aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg
    10921 ctgaccaaag aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat
    10981 atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa
    11041 aattgaaagc atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac
    11101 ctgctgctgc tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg
    11161 tcgctcattt tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg
    11221 ccgttgggtt agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt
    11281 tggtcgcatt tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga
    11341 atatcaaacc agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta
    11401 ttattgccgg attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga
    11461 tggctttatc tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt
    11521 tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt
    11581 ttgctaaatt cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc
    11641 gtttactgat ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag
    11701 caggaatcaa tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa
    11761 tgtatcaagc tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta
    11821 tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ctagcagcag
    11881 cttttgcttc attctttact ggtgtgacag aaccacttga attttcattt atgttcgttg
    11941 cttggccatt atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat
    12001 tcttccattg gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt
    12061 tgaaaaatcc gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag
    12121 tgatttacta ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc
    12181 gcgaggaagc agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg
    12241 cggcacaagc aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg
    12301 ataactgtac tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata
    12361 aaatcaaagc cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca
    12421 tcgtaggtac agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg
    12481 cagcacgacc tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtgaaacga
    12541 tcgaacaaga tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag
    12601 atgatgtatt cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag
    12661 aaatcttctc tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg
    12721 gaatccaaac agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact
    12781 taaaaggcga accattcact ttatatgtag aagaaggtca aaaagtcgca agaggtcaat
    12841 tgattgcttt agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg
    12901 ttgtattcac aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa
    12961 aagcaaatga taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac
    13021 ttttttatcg ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg
    13081 aataaaaacg gcgttctcct gccaactccg cggtaataag tccagccaaa cgaaacatat
    13141 gaagagctat tttttatttg actcttctta ttacttgatg cagaacagct ttttcacaaa
    13201 atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac attattcctg
    13261 atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca atactttttc
    13321 taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga tcgctaaaaa
    13381 catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa acgctgcatc
    13441 acttgcttca ataatttcta actctttacc tgtcgcacgc tctaatgcct tggctaattc
    13501 ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac tttctactag
    13561 tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt ttccatttcc
    13621 tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta attcattctc
    13681 tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa tcattttttc
    13741 agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg caaaacttgt
    13801 ataagcgata taagataccc cagctgcttg tgctgccttc actacattct tatgttcggc
    13861 ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt tcaacgcttc
    13921 tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag cttctttcag
    13981 tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg gaaccatctt
    14041 ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta ctaaataatg
    14101 catatttgcc actccctatt ctttttattt gttaagattt acagctgtac atttataaat
    14161 tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac
    14221 aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc tatattgaaa
    14281 tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc gaaacaaatg
    14341 cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa
    14401 ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta ctagatgtct
    14461 atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca aatccaaatt
    14521 gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa
    14581 aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa atagccgttt
    14641 tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta taattctctc
    14701 tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat tctccggggg
    14761 acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc gctgaagtca
    14821 tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct gaaaaatgca
    14881 aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag cgctactttc
    14941 acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc ccatatattt
    15001 cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg cgtaatcttc
    15061 tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta attcttcagg
    15121 cgattctgct aaagcgctta caacaagata cgttcctttt gcgaatggga tgattcgcgt
    15181 agtgtttgga acatcctgac tcgtttgaac accgacatag tacatcattt tcccgttata
    15241 gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg catctattgt
    15301 tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc
    15361 cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg gtttttcttc
    15421 gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat cttacataaa
    15481 attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat tttttaacta
    15541 tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat cctctcccca
    15601 ttttgtaaaa gagatctcgg gagcttcgac taactcataa tctgaagaag gaagccattc
    15661 tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt tattaggaaa
    15721 aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg gattttcttt
    15781 tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc ggtctccatc
    15841 aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact tatgcatctc
    15901 ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat tgactccttc
    15961 aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt tcttttcgat
    16021 acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga
    16081 tttttgtatg ccgtatctgc tgatttctga aggcagatat cctgaccaat cacgaaaagc
    16141 tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg tcactcttga
    16201 tccatctaga agatcgcgat tagccaaagc caatctacgg tttttgatat agtccattag
    16261 cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga ttcctgtatt
    16321 ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa
    16381 ttggttcaga tctttaagca tatttatctt cctccttgat tgccttttta tcatattatc
    16441 aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag aaaatgttgt
    16501 atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta ttatctgcct
    16561 atctatgttt tacctttaga aaaataaatt ttggggaaga cagattctaa gtaaacaaag
    16621 cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt acaaagcaaa
    16681 ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat acatcatacc
    16741 tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt ttgtcagata
    16801 aataaaaaac aaaaaaagtt ccatctttaa aacacgctat tcttttactt tttctcacat
    16861 tctctaagaa aagaaaagga gaatgaattt ttgacaattc gagcaaaagt gattatcaaa
    16921 tatgtaaaat cagctacttt tttcttaaac gtttctttat actaaatata aataagtgtt
    16981 tcatgaaagg agaactagat gaataattgg cttgagtatt ttcctgaaaa cgttttggaa
    17041 cgcggttaca gctatcatct gcacggtttt gtccgtcatt tgaactatac aagcaagtac
    17101 ttgtccgcaa cagtttctgg cacggaagat tataaggtag tgatcacttg ggatgaaaag
    17161 accaacatga cttgtgattg tctatatgcc atagaaggaa aaaaatgcaa acacatggct
    17221 gctgtcttat tcgcttacga agaacgtcca ataaaaaaat ccaactactc actttccgaa
    17281 ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac gggagctttt aacagagatt
    17341 cttatagaac atccgcactt tatcgaacgc ttcaaagtta agatgccatt tcatgcaata
    17401 aattattcag ataaatt
//
LOCUS       DACEPW010000045        16650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEPW010000045 DACEPW010000000
VERSION     DACEPW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.046"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW819_001180"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW819_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1244295.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IW819_001181"
     CDS             808..1242
                     /locus_tag="IW819_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1244296.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IW819_001182"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IW819_001182"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1244297.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IW819_001183"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IW819_001183"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1244298.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6191..8422
                     /gene="pflB"
                     /locus_tag="IW819_001184"
     CDS             6191..8422
                     /gene="pflB"
                     /locus_tag="IW819_001184"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1244299.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8666..9427
                     /gene="pflA"
                     /locus_tag="IW819_001185"
     CDS             8666..9427
                     /gene="pflA"
                     /locus_tag="IW819_001185"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1244300.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9512..10438
                     /locus_tag="IW819_001186"
     CDS             9512..10438
                     /locus_tag="IW819_001186"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1244301.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10620..11513
                     /locus_tag="IW819_001187"
     CDS             10620..11513
                     /locus_tag="IW819_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1244302.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11647..11772
                     /locus_tag="IW819_001188"
     CDS             11647..11772
                     /locus_tag="IW819_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1244303.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11906..12538)
                     /locus_tag="IW819_001189"
     CDS             complement(11906..12538)
                     /locus_tag="IW819_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244304.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12686..13228)
                     /locus_tag="IW819_001190"
     CDS             complement(12686..13228)
                     /locus_tag="IW819_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1244305.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13363..13752
                     /locus_tag="IW819_001191"
     CDS             13363..13752
                     /locus_tag="IW819_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244306.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13756..14520
                     /locus_tag="IW819_001192"
     CDS             13756..14520
                     /locus_tag="IW819_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1244307.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14649..15596
                     /locus_tag="IW819_001193"
     CDS             14649..15596
                     /locus_tag="IW819_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1244308.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15726..16466
                     /locus_tag="IW819_001194"
     CDS             15726..16466
                     /locus_tag="IW819_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244309.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         5877 a         2817 c         3456 g         4500 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtga cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaagatgaaa gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt
     6061 taaatatatg taaataaaac ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa
     6121 gatagggtgc tgtgttatac taatcacgta aaaactgtta cataaaacag aaatttagga
     6181 ggtccataca atggagcaat ggaatggatt taaaggtacc aaatggaaag aaggcgtaga
     6241 cactagagac tttattcaaa ataactacac tgaatatcgc ggtgatgaca gctttttgga
     6301 aggcccaaca gaagcaacag aaaaattatg ggatgaattg caaaagttaa atgatatcca
     6361 attcgagcgt aacggtgtct atgatatgga cacagaaatc gtatcaacta ttacttcaca
     6421 cgatgcaggc tatttggata aagatttgga aaaaatcgtc ggtctacaga cagacaaacc
     6481 tttgaaacaa gcattcatgc cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag
     6541 caatggctat acaccaaacg atgaattgac acatattttc actgactggc gcaaaacaca
     6601 taatcaaggt gtatttgacg cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat
     6661 cattacaggg cttccagacg cttacggtcg cggacgtatc atcggtgact atcgtcgtgt
     6721 ggcattgtat ggaatcgact atttgatgga acaaaagaaa aaagatcatg atatgactgg
     6781 ttatcataca atgagtgaag aagtgattcg tttacgtgaa gaaatcactg aacaatatcg
     6841 tgcattagcg caaatgaaag aaatggctgc gaaatatggc tatgatattt ctaaaccagc
     6901 tgcaaatgca aaagaagctg tccaatggct atacttcgga tatttagcag caatcaaatc
     6961 acaaaatggt gctgcgatgt caatcggacg tatctcagca ttccttgata tctatatcca
     7021 acgtgatttg gataatggag taatcacaga aaaagaagca caagaattga tcgaccactt
     7081 agtaatgaaa ttacgtatgg tcaaattcgc gcgtacacct gaatataacc aattattctc
     7141 aggattccct atctgggcta ctttatccat tgcaggtatg ggattagatg gtcgttcatt
     7201 agttacaaag aatgatttcc gtctgcttca tacattaaca aatatgggac cttcaccaga
     7261 accaaacttg actgttttgt attctgaaca ccttccagaa ggattcagaa catatgctag
     7321 cagaatcgct atcgaaagtt cttcgattca attcgaaaat gataatcagc ttcgtgaaca
     7381 atggggttct gatgattgtg cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga
     7441 catgcaattc tttggcgctc gtgctaactt ggctaaaaca ctattatatg ccatcaatgg
     7501 cggagtcgat gaagtgactg gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga
     7561 aatactagac tttgaagaag tgaaacataa cttcatgaat atgatggatt ggttagcaga
     7621 actatatgtc aatacgttga acatcattca ttatatgcat gataaatatt cttatgaagc
     7681 agctcagctg gctttgatgg attctgactt gcaacgcaca tttgctacag gtattgctgg
     7741 gatctctcat gcggcagaca gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg
     7801 taatgaaaaa ggaatagcag ttgattttga agtggatggc gacttcccta aatacggaaa
     7861 tgatgatgac cgtgcagatg aattagtttc atggatcttg gaatacttca tgggacaaat
     7921 caaacgtcat aaaacttacc gcaactcaaa accaacaact tcattattga cgattacttc
     7981 aaatgttgtt tacggtaaaa atacaggaaa tactcctgat ggccgtgaag ctggaaaacc
     8041 acttgcgcca ggagcaaatc caagttacaa tgcggaacaa agtgggttat tagctagcct
     8101 gaactcaaca gctaaaatga attacggctg tgcattggat ggaatttcaa atacccaaac
     8161 aatcaatcct tcaggtcttg gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt
     8221 cttagatgga tacttctcaa aaggagctta tcatttgaat gtcaatgtat tcagtaatga
     8281 tttattgcgt gatgcaatgg aaaatccaat gaaatatccg aacttgacga tccgggtatc
     8341 tggttatgca gttaaattcc gtgacttgac aagagaacaa caattagatg tcttgatgag
     8401 aacttcacac gacagaatgt aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa
     8461 ggacgttgaa tagccatcgc atcaaaatcg aataatcagt atgcttattt gaaagaaacg
     8521 atttacaggg gcaggacttg tatgttccgt ccctgtaaat tttacacata acgaagcggc
     8581 aattgcgaaa aattttgttc agaaagcttc gcttcgacag ctaacttgta cttaaaatga
     8641 aatcaaagaa aagatgtgaa cttaaatgga agaaaaaaca atcggctatg ttcattctat
     8701 cgaaactttc ggttcagtag acggacctgg actgcgcttt gtcgtgttta tgcaaggatg
     8761 ccgcatgcgt tgccaatttt gtcacaatcc agatacttgg aatattggcg gagggaaaga
     8821 atacacagca gatgaacttt tagataaagc agaacgcttc agaccttact ggggagataa
     8881 aggtggtatt actgttagtg gtggagaacc tctattgcag atcgattttt tgatcgaact
     8941 atttaaaaaa gcaaaagagc ggaagatgca tactacattg gatacatgcg gaaaaccttt
     9001 tacgtatgaa gaacctttct tttcacgttt tcaagaattg atgaagtata cagatttact
     9061 gttatttgac atcaaacaca tcgataatga agagcataaa aaactaacgc attgggacaa
     9121 tgaaaatatt ttagaaatgg ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg
     9181 ccatgtacta gttcctgagc gaagtgatta tgatgaatat ttgatccgac tagacaactt
     9241 tattcaaacc ttgtcaaatg tagacagagt agagattctt ccttatcata cgatgggaaa
     9301 atacaagtgg gaaacacttg gtttgaaata tccattagaa gggatcgaac cacctacaaa
     9361 agaacgtgtc gaaaatgcta aacgattgct tcatacagaa gagtatacaa aatatttaac
     9421 gcgataggtc gcatcggaaa gaagataagt attgcttctt tccgtttttt tgctattatt
     9481 aaataggaga aatcacaaag gagcgaatga catgtcaaaa attttagttt ttggtcacca
     9541 aaatccagat acagatgcaa tcggggcagc cattgcgttt tcacacttgc aaaatgaatt
     9601 aggtaaagaa acagaagcag ttgctctagg tacacctaat gaagaaacac aatacgcatt
     9661 agaccacttt cagctatctg caccacgagt agtagagtca gctaaagcag aaaatgcaga
     9721 tgtcatgctt gtggatcata atgaattcca acaaagtatt tcagatatca gcgaagtaaa
     9781 tattttagct gtagtagacc accatcgtat tgcaaacttt gaaacagcta atccattgta
     9841 ctatcgtgca gaaccagttg gatgtacaag tacgattatt ttaaaactat ataaagaaaa
     9901 ccaagtaaca attcctaaag aaatcgcagg aatcatgctc tcagctatca tttcagatac
     9961 attgttgttc aaatcaccaa catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc
    10021 agcaatcgct gaagtcgatc taaacactta tggacttgaa atgttgaaag caggaacaaa
    10081 tctaagtgat aaatcagctg cggttcttct tgatttggat gcaaaaagtt tcccaatggg
    10141 cagttccaac gttcgtatcg ctcaagtcaa tacagtggac cttaatgaag tgatggatcg
    10201 acaagcagag ctagaagcag caatggaaga tgaaatgaaa gcaaatggct acgatctgtt
    10261 cgtattagtt gtcacaaaca tcttagacag tgattcagaa ttacttgttg ctggtaaagg
    10321 aaaagaaaaa gtagaagaag ctttccaaac aactttgacc aacaaccgtg ctttgcttaa
    10381 aggtgtcgtt tcacgtaaaa aacaagtcgt acctcaattg acggctgtat ttagctaatc
    10441 aaatggtagt atgaagcatt caaaatgaat ggataagcga ttgatataaa aagaaacata
    10501 gaacatacta aaggccgaag aaatggagga gaataacagt caattatgac tcttgttctt
    10561 cctccttttt acgtaaaagc cttttaagta aaataaaacg gatagaaaag agaacatata
    10621 tgaactatta ttcaaaaaat aaggaaaact tttatcaaaa actgaccgat gatccgcttt
    10681 tttcttcatt gaccgattat ctatatgaac atcgagaaca agaaacgatt ctaagagaat
    10741 tgaaaaaaga attttcgcaa aataaatttt cccattttct tgatttattg atcgatgcag
    10801 gattaatcaa gagggaagaa cgaagatatc atttgaactt tccaatattt gatcctaaag
    10861 attacttgca acaagccact tcggctgctg aaaccatagc tgaacaattg aagagactct
    10921 ctgttgatga acaaaaatta gcgatgggag aagttatttg ggcgtattgt ttcgaagatg
    10981 aacggaaaga agcgtatttt tatggagtaa gaaacagccg agagacagaa ttgctgagga
    11041 caactgcagg aaatgagaag taccgtttta tcacactttc ctctatagaa cacttcccgc
    11101 ttactttggc taattacttt tttgtacaga agaatcagct tcctgtaacg aaggcattca
    11161 aagaattagc cgaacttatt ggagacgtaa atgaatcgta cttttttgat caaatagaag
    11221 ttatcgttga caggatacgt aagaataaat ataaaaatcg ccggcctagt attttccatc
    11281 agtctctact tgtgacgaat actataaagg aagaagaatc attcacatta gaattaccta
    11341 ttgtcgaaaa aaacaatttt gagattgaat taccaacatt ggatccttca ttgacaatgg
    11401 aggaaatggc ctttcttaaa cgacaaatct tttctgagct gagcaagaaa tttataccgc
    11461 atgcattttc ttatatcaaa gaatatggaa cagttttagt gagtaaaacc taagagagca
    11521 gtaagcaaaa attatactga ctcttgcagg aggagccttt ctcttccttt atggttaaaa
    11581 gaattatttg agtattttgc ctatctacca cctattagat tcactacatg gtctggaaac
    11641 aaaggaatgc aaaaaaatgg tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa
    11701 gttagatttt taaataatca atattatgat tcagtcaagt atggcatttt atgggaagaa
    11761 ctagctgatt agattaggaa gtccatttga taatccagaa tcactaagaa acgaatggcg
    11821 agtcattaat aatttatcca atcttgggtt attcttttag attgaagtgt ttaaaactaa
    11881 aaaagcattt atgaagtcgt aatagtcagg cttcaaaatg ctttttattt tgtttgtttt
    11941 ttctttgcca cataaataca cctagactga cgatgaagag catatccaac agatttcctg
    12001 cgacttggat cgtcgaaggg ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca
    12061 agctgtaaat aaaacaggtg acaggaacta atcgaaagaa gagcggacat ttcaattggc
    12121 taaaaatgag tgaaaaaata aaataagtaa aaatggataa aatcacgact aaaccattca
    12181 tgataaagaa gaaaagcgta ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa
    12241 acgttttaaa tgcaatcaat acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa
    12301 aaaaataggc accgaaataa acagaaaaca gtaagccgca aagagaagac aaaatcaaaa
    12361 aaaacggatt ttcttcggct tctgtgatat cagaatgatc caaaagcaac cagcctaatg
    12421 cactagaagg cgcatgtgag gtcttaaggt atgaataaca tagaaaaaat aagatcgtca
    12481 aagttgcaaa aaaagagcct aaatgtaaaa aattagtggt tctacgttga atttccataa
    12541 tatctccaaa ttaaattgat aagtatataa agtataataa caaattgatg agtatatgtc
    12601 gatgtttttt aatcaaaata ataaataaaa actgtctaac atttaaaatt taatttagat
    12661 attagacagt ttttaagttt gtgagttatt ttccttgtcg ttttctcact tttttagtcc
    12721 agaaaccacc tgaaatatat tttggctcat aagaaatgat aaaagcacgc tcttcgattt
    12781 ctttgatcag gcgatacagt tcacgttcgc tttttctagg agaaagtatt tcgagaacta
    12841 gccgctcacc ttccagcccc attgcagaag tctgtgtcac accataaccg tgttctctca
    12901 gtatacccgg caaactttta tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga
    12961 tgtatcctaa tgccaatttg tcttcaatca aaattccgac actgatacct actgcgtaac
    13021 ctaacgcata gacaaataaa ttcagtggat tatctaaacg atctaaaaca agggataagc
    13081 cgacgatata aatcgtgatc tcgaccatac tgacaagtgg agcaatcagt cgatagcctt
    13141 tcattgtcaa catgaaacgt aacgtgttca gagtgatata agcaaaatta atgaggaaga
    13201 tcatccccaa catttttgga tcaatcattt gtaaaactcc tttcatttat ataacaacaa
    13261 tatcaaaaaa aatcgtaagt gaagaaaaat tgaaacttta tacatacaaa aaaatgataa
    13321 aatgattata atagaattat aaagataggt taggtgatta aaatgaaaac ggtcaatcgt
    13381 gtgttttgtg ccagccacgt gctaagtctc cttctcagta tatacttagg tgtctattcg
    13441 attcaacagc caattgaggt agagaacatt gtttttcaga tgtcgctgac agatggttta
    13501 attttaagcg tttttttctt tttgatactg tttattggta atattttcgg tagcgttgcc
    13561 agttttttga atttttctat ttatccactc ctttcgctag cattaggagt ggtgggactt
    13621 tgttctctgt gcttatttcc cgagccattc agtcctgcat ttatattatt tggtacactg
    13681 aacctgttcc agacaactgt tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa
    13741 ataggtgagt aacaattgaa tccaattata ttgaaacgtt ctgtccatca tattccagtg
    13801 ttagaagttg ttctgaaaga attaagagag gaacgtattc ccgttgtcat ctattatcac
    13861 ggatggcaga caagtaagga gttagttttg acgcaaggaa gaaaaattgc gcagcagggc
    13921 attcgtgtga tcctgccaga tgcgatgaat catggggagc gcaaacagcc ggtttcttct
    13981 attccatcct atacattttg ggatagtatc tacggaaatc tgtttgaatt tgacacactt
    14041 atcaagcatt tagaaaaaag aggtcttcta catgatcagc ttgcagttgg cggggtttcg
    14101 atggggggaa tgacaacatg cgccttgttg gctaaacatc ctcatatagt agcgggcgtg
    14161 tgtttgatgg gttcccctgc acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg
    14221 gaatataatt atgtcgtacc aaaagactat tttgatttga tttcatgggt taagagctat
    14281 gatttgtcat tgcagccgga aaagttagct ggacgtcctc tgttcttttg gcatggacaa
    14341 gctgacgaaa aaatccctta tatacaaacg gaacgatttg tagaaaaaaa tcggcataag
    14401 tcttacggaa aagaaattgt ttttcgttcc tcaaaaacac agcgacatat ggtacaaata
    14461 ccactaatgg aagaagcttc cactttttta gcaaaaaatt tgataaaaaa gtataaataa
    14521 ttgaaacgga aaatcactca tgacgataga ggattttccg ttttttattt tgggctataa
    14581 taaactctgt taggaaaatt tggtataatt gatagtaaga atgacaacat gttagaaagg
    14641 aagagagcat gcccacttta gcagatgttg caaaacgtgc caatgtttca aaaatgacgg
    14701 tctctcgagt catcaatcat ccggaacagg tgactgacga attgaaagag ttagttttct
    14761 cagcaatggc tgaactggat taccgtccaa atattgctgc aaaagcactt gtcacgaatc
    14821 ggtcgcagat catcaaatta tttatactgg aagaaatcga cacaactgag ccctattaca
    14881 tgaatttgct gatgggaatc gctaaacgga tcggtaaagc gcattattct ctccaattag
    14941 ttacaaatga tgccttcgac gtcggttctt gtgatggata catcatcaca ggtgtcagaa
    15001 aaaaagactt cgattggatc gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt
    15061 ctgaaggata tgattatgta gacagcgata atgaatatgg tacagcaaaa gcaacgcaat
    15121 atgccttaga aagagattat caaacgatta tttatatcgg tatcgaagtg gacgagcctt
    15181 ttgaaatttc aagggaaact ggttatttat ctgtgatgaa ggaagttgat cttcctagta
    15241 aaatcttgcg gtttccaaat cattcgacag aagtagaaag atatatgaca gaaaactggg
    15301 cagattttac agataaaact tgttttgtat gtagttcaga tcggctagca ctaggagcag
    15361 taagagggat tataaatgct ggaggacact tgcccgaaga atttggtgtg attggatttg
    15421 atggggtatt tttagaccaa atcagttcac ctaaattgac aacgatcaag caagatatta
    15481 tacgtatggg tgaagtatgt ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga
    15541 gtcaaggtta tcgtcgtttt tttcctgaac tagttatcag agagacaacg aattaagtct
    15601 tattactata tatatattta tgaaattcat cgctattttc ttaaaataat cagcgcattg
    15661 ctactgaaaa caatgaatct gtgctattat tgagaaaagt tccatttttg agaaggatgg
    15721 tttgaatgaa agaaatcaga cgaaaacatt tccgttttcc tgcgtacgat gatgagttag
    15781 gcgtaaaatt aaccaacgac aagaagcgag tattatttga cggacaagaa gatcttttga
    15841 ctgaacagaa tcagtccccg tttgaacaga tgaaagaatt tcatttcacg aacacgaaca
    15901 ccgagaaaat ccgtcgcaag cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg
    15961 aaaatttgga aaagcataaa ggaaaattac cggattatac cggccattac caatcagaaa
    16021 tcacatcgac aggacgaaaa aaactttttg gtgattcctc tcgttctgtc agacaaagtg
    16081 aaacaaggac agagtccaag catactacac gaaacgcatc tgttgacacg aaggtgcggg
    16141 agcgttctta ttttgttcct aaatatgtac ccgcatcgat tatcccagac cctagagaaa
    16201 gcaaaatttc tgaggatcat ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt
    16261 tgttcgatac agaaccgacg ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa
    16321 cattcaatcg aacaaaaacg ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa
    16381 aaagccatgg tatcttagaa cgctcattac aaggattgat cgatgatcaa ggaaactctt
    16441 tgggagaaaa tagctatttt aaataagtga tatacaaatt tatattcggc tacagttgga
    16501 aaggaaacgc tgcggcgttt ccttatttta tactctatcc gaaagtttat atcgtatcct
    16561 caaatttttt tgagtttaag cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa
    16621 taaaaaaaca aactattttc gcagatcgtt
//
LOCUS       DACEPW010000046        16517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEPW010000046 DACEPW010000000
VERSION     DACEPW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.047"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            329..658
                     /locus_tag="IW819_001195"
     CDS             329..658
                     /locus_tag="IW819_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244310.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            645..986
                     /locus_tag="IW819_001196"
     CDS             645..986
                     /locus_tag="IW819_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1244311.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            1100..1339
                     /locus_tag="IW819_001197"
     CDS             1100..1339
                     /locus_tag="IW819_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244312.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            1406..1645
                     /locus_tag="IW819_001198"
     CDS             1406..1645
                     /locus_tag="IW819_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244313.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            1772..2461
                     /locus_tag="IW819_001199"
     CDS             1772..2461
                     /locus_tag="IW819_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ1244314.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            2467..3633
                     /locus_tag="IW819_001200"
     CDS             2467..3633
                     /locus_tag="IW819_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1244315.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            3715..4386
                     /locus_tag="IW819_001201"
     CDS             3715..4386
                     /locus_tag="IW819_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1244316.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            4439..6415
                     /locus_tag="IW819_001202"
     CDS             4439..6415
                     /locus_tag="IW819_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1244317.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            6428..7180
                     /locus_tag="IW819_001203"
     CDS             6428..7180
                     /locus_tag="IW819_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1244318.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            7196..7957
                     /locus_tag="IW819_001204"
     CDS             7196..7957
                     /locus_tag="IW819_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244319.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            7970..8230
                     /locus_tag="IW819_001205"
     CDS             7970..8230
                     /locus_tag="IW819_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244320.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            8227..10317
                     /locus_tag="IW819_001206"
     CDS             8227..10317
                     /locus_tag="IW819_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1244321.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(10353..12410)
                     /locus_tag="IW819_001207"
     CDS             complement(10353..12410)
                     /locus_tag="IW819_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1244322.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(12555..12746)
                     /locus_tag="IW819_001208"
     CDS             complement(12555..12746)
                     /locus_tag="IW819_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244323.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            12934..13389
                     /locus_tag="IW819_001209"
     CDS             12934..13389
                     /locus_tag="IW819_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244324.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            13386..13628
                     /locus_tag="IW819_001210"
     CDS             13386..13628
                     /locus_tag="IW819_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244325.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            13646..13948
                     /locus_tag="IW819_001211"
     CDS             13646..13948
                     /locus_tag="IW819_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244326.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            14017..16071
                     /locus_tag="IW819_001212"
     CDS             14017..16071
                     /locus_tag="IW819_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244327.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            16071..>16517
                     /locus_tag="IW819_001213"
     CDS             16071..>16517
                     /locus_tag="IW819_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAQ1244328.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLK"
BASE COUNT         5812 a         2572 c         3365 g         4768 t
ORIGIN      
        1 ttagacaagt tagaatagcg cctagattaa tcatgagcag ctactaaggg atctaggctt
       61 tgacgcaaac tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa
      121 ttgtgtcgtg tgctgcagta catgtagata cttgcgagga cgttgatcat tggatcaatc
      181 gagcatttac cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgattta
      241 ttttatatat ttttgagtga acgaaaaacg ataaagatat tcgttatgaa gtaaaactaa
      301 tttagtagat agcaaaggtg gtagaggaat gattcaaaaa tttgcgaaaa tgagtaaaac
      361 tgcatggatt gaaaagcttg aaaatgactt gagtgtcatg cgattagctc caaagaaatt
      421 caaacgagag attgcagaaa aagaagcaat tttgtcggta ttgaatgcgg tagacgataa
      481 atgtccagta aaaattagtt tttcagatat agcaacaatt atcgaaggta tttattatgg
      541 tgaagttgat gaagagatat cttctacaaa agatttatta gattttttgg ataaaaacaa
      601 agataccttt atagtactaa ctgattatga ggaggttaca acaaatgaaa aattatgatc
      661 cgaatatacg attgggcaca catacgatta aggtttcttt ccaacgttgg gattacaaag
      721 gatttgtgac gtttagaaga ggcggaaatt gtaaaggatt agatgtttta gcattagacg
      781 aggatgattt atacgatcaa aaattgactg ataatccaat tggattcggc ttgctacctg
      841 aagatgatga aggtaatgaa tggtttaaaa tgacgcttac ggatgataat ggagacgaat
      901 tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcgtt gaaatcattg
      961 aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt
     1021 agaaggagtc acgacgaata ataaaaaggt agtattacct taggcattga gtaaggaatt
     1081 tatttaggag gaaaattaga tggcgcgacc agttttagag gcgatggata tggatacttt
     1141 gacaaaagga aggtatgtac agctgttagc tcgtggttat tcacctaaag atgtattcaa
     1201 atccctgtct aaaggaacag atatggaaaa agaaagatta cgaaaagaat tcgattattg
     1261 gagaacccat aatggaatca aggatttgaa accagcacgg ccagtaatta aacgaaagaa
     1321 aaagataacc caagactgaa gtgtagagcg tatgtgatca taggcttttt tatttttgca
     1381 aaaaacaaga aaggtaggaa aactcatgaa ttatgaagaa atcgaaaaca gaaaaaaagt
     1441 gtcgaaagaa atggaagaaa agttgctaaa aatgatgaaa caaaaacacc ttaaaagatt
     1501 atctgtcatg caatatatca atgatatgaa gataacagga aaagagaaag cttgtttgtt
     1561 aggttcaatg aaaaactttg aacagttgag gcgaacttat gtaaaaacag gttccaattg
     1621 ccaactatta ttagaagttt cttgacgatt atgatatgaa aatgatcaat tatgatgtgg
     1681 cattgttatt gaataaaaag aatagtaaaa tttaacatgg tttatcttgg ttagatgtag
     1741 gtaaattaat tcatatatag aaggtagata gatgaataaa aacaataaaa ctttatttaa
     1801 tccttacgtg ggcgtaatca ttgtttttct tctttttagt tattttgctt atcaaggtta
     1861 tcaaaaggac aaaatcgctg aaaaagtaga tggtgtaaca aaaacaatcc atattcagga
     1921 acaggctaaa acctatcaag ataatttaac cagtttaacg gatatttcta agagacaact
     1981 gattactaat caaaaacagg cattagaaaa ggatctcaaa ggtgaaattg ctggaaaact
     2041 tgttttttct tctacaggat ttattctccc tgtttttcaa ggagcgaata gcgacacgct
     2101 tagtttagga gtagcttcta cttattatct agatactgaa atgggaaatg gaaattatat
     2161 tcttgctggt tataacgtag atacaccaag cgttttatta tccgatatga ctgatttatc
     2221 tgaaggagaa gaaattgatc taatcgatgc tcattacaag tatcagtatg aagtctccaa
     2281 aagaggaaaa atttctgatt acagccaggt agttcaatct aaggaaaaca attccttttt
     2341 atccttgcca aaagcaggta aaaaaccact actcacactg ttagcctttc aagcaaataa
     2401 tatgaaaaag tgcgatgtga tccagtgtga attagtcaat gtagaaaaaa tcaataaata
     2461 agggaagtga gtcacattca agaacttgta gaaaaattga tcaataaaat gagtgccatt
     2521 tttctgttac tgttggtatc cattctatat ttaatatgga cggtggggtt aaatcctcct
     2581 acgatattat ttatttatga taatgctgca cagttagtga cgtttttcgt aggctgtttg
     2641 tttcttttga atctgtacaa ggtaaagaga aacgatctat tcttcattgc agcaggcatt
     2701 cttttttacc ttttcttttc tttttttaga agctaccgag gagatacttt ctatggtgat
     2761 ctgatcgttc cgacaattat tgctattatt ttgattgtaa aatggacgaa atttactgta
     2821 attgatcgta gcttgtatct tgtagtattc tatgtctttt tactcgtaac ggtttatcga
     2881 gtatttactg agataccagt accagaagga caaagtattt ggaatccatc gaacaaatta
     2941 tcagatattt ggatcaatac aaatacgata ggtagttctt taatgacatt agcccttctt
     3001 atcacaggat ttgctagttc atttggaaaa tggtacatgc gattgctatc attaccagcg
     3061 ttgattcttg ctggggcaac gatttgggtt tgccaatcga aagcagcatt atatgcgctt
     3121 gttatcttca ttatcttaga aattttacct aagaaggttt ttcgaaatac acacttgtcc
     3181 ttttggatgt atgcagcagt ttcggttcta gctttaccga tttcttattt tgctgcaatg
     3241 tcagacaaag ttcacttatt tacaggacga gaagaaattt ggcgcaaatt ctatgaaaca
     3301 ttaggcgaaa aaacaggaca aatatttgta gggatgaaac ctttcttttt ccatagagga
     3361 aatcagattc taggtaatca taatacgtat cacgcactcg tcaatcacta tggattgatt
     3421 ggttttgttc tatcgattat tcttttgctt agtttcttat ggttgctcgt aagaaaacgt
     3481 gagctgacaa atggacaact aacattcttg tgggcttttt tagcgatcat gtgtcaatcg
     3541 ttcatggaag aaacattaac gacaatcagt tgggtgccaa ttgtattctt aatgttagca
     3601 atggctacac accaatataa aaccaccaga taaatgaaat agaatagtaa taagagaaaa
     3661 cactttttcg ttgtgaaaaa gtgttttttg ttttgtctga aaggaggcag atggatgaaa
     3721 aaatggttat ttggtttttt aggagtcgct ttaatcgtcg tttgcagtgt gtttggctat
     3781 gttagctacc aaaaacatga aggagaggtg tttaagcaaa atatagaaaa aaagatgccc
     3841 gtagatcaaa tcaatgcaca cgcaaagagt tataaggagg atgcaacgaa cgtcaataat
     3901 gatatgtctt tggggcaaat gttgtccata caaaaagaag caatcgaaat gggcgtcaat
     3961 aaacaagtat ttgcacaaat tcagattcct gcacttggtt tagcattgcc aatttttaaa
     4021 ggggcaaatc aatacacttt gagtcttgga gcggctactt atttttatga agatgccgaa
     4081 atgggtaaag gaaactatgt cctagcaggt cataacatgg aaatgccagg tgttttattt
     4141 tctgatattc aaaagttgtc attaggggag gtgatggact tagttagtaa cgatggcgtc
     4201 tatcgttaca aggtaacgcg taaatttatc gtacctgagt actttaaatt aatcgatggc
     4261 gttcctgaag agaactcttt cttgtcttta ccaaagaaag gagagaaacc cttattaacg
     4321 ttgtttactt gtgtttatac gtcacaagga aaagaaagat acgtggtaca gggagagcta
     4381 caataacaag aatttaatca acgcgagtcg ttgaaatatg aaatgaggag agtaaaaaat
     4441 ggcaataaat cggtatgtaa aaaaagctgg aatgattgtt ggaatgttag gaatcattgc
     4501 aggctgcttt caggggtatc gagcttatgc agaagatgtc acgcaaaaaa cgccaccaga
     4561 aagggtcaat atcacggttc ataaattaat gtacgatcaa agtacgcaat tgaacgttga
     4621 tattgacgga atcaaaaatg atggttatac gcacgatcaa tatcctacag gtgtaacgaa
     4681 atacaacaag gcagattatg gtgatgttga gtttacgtta ggaaatatta cggatcaagt
     4741 attaccgact gaagattctg atctaaccaa cgcgaaagtg gacgaaatcg ttaaagatgt
     4801 agaagacaag ggatctaaca gtgagtacgt taagaatgca accaataaaa ttacttcagc
     4861 tgtagatgaa aatggtgaga tcacttttgc tgatcaacca gcctatgtca attccaaagg
     4921 gaatgtatat gtggtttatg aaagtaaatc cgctgctgga ttagtcacac aaaaagcaaa
     4981 accaatggtt gtgattgcgc caatgacaga caacacagga tcaggatttt taaaagatat
     5041 tcatttgtat ccaaagaaca ttgtttctaa gttgagtttt gaattaacga aatttggtga
     5101 cgatggcaca gctcaaagca aacaaacacc tttaaaaggg gcaaaatttg agttatacaa
     5161 gggcgaacct ggaaaaggaa caaaattagg agagcttgta tcggatgatc aaggaaagct
     5221 gactgcaact gatttgacgt taggaaaata ctactttgtg gaagttcctt cagaagttgt
     5281 agttggatca gatcaagaac caacagcaga ccaatatttg ttaggcgcag acgctcgcaa
     5341 cgatgcgcac aataagttaa cctttgaaat tacgaatgac ggcgtgacaa gtgatttaaa
     5401 agcttcctac gtgaactata aagcaccagt gatcgataaa acagtgacaa atggaacagg
     5461 agaagaacat tctttccaaa ttggtgacgc agtgaattat caaggtacaa ttcatatccc
     5521 tacagatatc gcaggtggaa cagatgggat tacagtaaat ggtgtaaaaa gtgaaacttc
     5581 tccttacagt gtatttaaat ggggcgacac agcaggtaaa ggactttctt atgttgctga
     5641 aaaagcagat attaaagtaa caaataatga tggttcagtc gtattaaaag aaaatactga
     5701 ctataaaatc gaaaattctg atagtggttt tgtgatcgat tttatcgtaa ataatggaca
     5761 agtaagtgat acagttgcga atttacatgg tcaagatcta aaaatgactt ataacatgtt
     5821 tgtgaatgat agtgctgaaa tcgcaaatcc attaacaaat agcgtggatt tcgtttataa
     5881 caataatcca tttaatcagg aagaacacca cgaaaaaacg aaagctgacg ttgtgactta
     5941 tggcgcaaaa ttcttaaaag tcgatagcgg cctatttgga acaggtatca aagcaacacc
     6001 gcttgaaggc gcagaatttg cagctaagaa tgcagaaggt aagtactacg gtggacttac
     6061 tgatacagat aaagatggtg tcaaagaagc agtttgggta gacgatgtag caaatgcagc
     6121 aatcctaaaa tcagataaag agggtcattt tgaaatcact ggtttaactg aaggagatta
     6181 ctcactggaa gaaacaaaag ctcctgaaaa ctatcaaaaa ttaacaaaag agatctcttt
     6241 caaagtagac aaagattcat acaaagaaga aaaccgaatc acgatcaaaa ataatcaaaa
     6301 agcttccgta ccaatgactg gtagcaacgg tttccaatct tacgtattga tctcttgctt
     6361 gttgcttggg gcaggcgcaa tttctgcagt tgtttacttt aagaagaaag cctagaagga
     6421 tcagctaatg attatcagac accctaagaa aaagcgaatc atgggtaaat ggatcattgc
     6481 tttctggtta ctttcagctg ttggtgtctt acttttgatg cctgctgaag cgagcgtggc
     6541 gaaataccaa caaaatcagc agattgccgc aatcgatcga acaggaacgg cagcagaaac
     6601 ggattcttct ttggatgttg ctaagattga gttaggcgat cctgttggta ttcttacaat
     6661 tccaagtatt tctttaaaac taccaattta tgacggaact tccgacaaaa ttttagagaa
     6721 tggtgtaggg attacggaag gaactggcga tattacagga ggaaacggta aaaatccttt
     6781 aatcgcaggg cacagtggac tttataaaga taatcttttc gatgatttgc cttcagttaa
     6841 aaagggagaa aagttttata tcaaagttga tggtgagcag cacgcttatc aaatcgatcg
     6901 gattgaggaa gtgcaaaaag acgaactaca aagaaacttt gtcacttact tagaaccaaa
     6961 tcccaatgaa gatcgagtaa cgctaatgac atgtacgcct aaggggatca acacacaccg
     7021 tttcctagtg tatgggaagc gagtaacctt tacaaaatca gaattaaaag atgaagaaaa
     7081 taagaaacaa aaattatctt ggaaatggct tttaggaagt actgtttttc tttcagtcat
     7141 gatcattggt tcgctatttg tttataaaaa gaaaaaataa aggagggtaa ccaacatgag
     7201 aaatagaaaa tggctatttg tttgtgcagc cgctcttttt tctttcactg cctacacaca
     7261 gttgacgttt gcagacagac aagaaagcac ggatagcaca gaacaaacaa tccaaacgtc
     7321 agatagcagc acgattcaaa gcagcgatga aaccgtcgat tcgtcaacag ttgattcttc
     7381 agaagaacct aaaattgctg aagacgatca aatagtcaaa cagaccattc acgttcaaaa
     7441 aattatttct tccgaacaat tagacaacca ggggcaaaag cttgaaaaac aagaaggagt
     7501 aaatggcgcg aagttttctg tttatgatgt atcggacatt ttacaaaaga tggatgttaa
     7561 ggatctcact actgaccaaa ttgaatccca attaaaagat cgagtgaaaa aactatccag
     7621 tgatcaactg aagctaattt ctaatggaga aacaaagaca attgatcaac aaacaggtgt
     7681 ctttgaattc tcggtagaag tacaggcaaa tcagaaacaa gcttattata ttgtcaatga
     7741 atcaagtcct gaaaacattt ctaatagcga ggatattttg ctattaacac ctgtttctga
     7801 taaaaatggt gaattcttaa aggatgtgtg gatttatcct aaatcagaag cttcgcaacc
     7861 taaagaagaa gtaaaaaaaa tcgtatctac tggcgtaaag aaaaacttct ttgagaattg
     7921 ttgggatttt gttactcact tgttttctag agattaagga agaaataaaa tgaaactgat
     7981 caaattatta ttattcaatt tttcttgttt aaccgcagtc tttttgtact gcggtttatt
     8041 attttctaaa ctatctattt tgccaagcta ccaactatac ctaatgagga ttttaaacat
     8101 accagctgct tccatgccgc tagatattat ggaaaagctc attaggaatc cattatttac
     8161 tacgatcaat ttgatcgtta ttgtcggaat ttcactagtc atttataaaa aagaggggat
     8221 gaagggatga attttaaaca ctcgtggaca agacggttaa aatatgtttt tcccataatg
     8281 atgatgttag gtacattgtt tgggttaaaa acagccaatg tctttgctga aaaagttatt
     8341 gtcgatccgt caaatccaat ccagaacgta aagaataatt ttattattcc aaaagatgtt
     8401 cctaatgcaa aaacaaatat tactgtaaac ggagcgtcaa aagtttacaa atatcatgtt
     8461 gatgccaaca aaggtggttt tacagacgac tacgtaggat tagtggaggg aaacaaaaat
     8521 atacgctatc cttctttcca aaaagaatac ggtgaaacaa accttgtttt agaaggcgta
     8581 acaaccaaca ccaaagtcaa tattgactat ggaaaaatcg gtacgtataa cgggaaaaaa
     8641 gtcaatatca agctagttct atctaatatt cacttatact cggatacgtt gccgtggaat
     8701 ctactagata acaactatac aaaaactcat tttcgagacg atggctataa aaatacgaat
     8761 ggtgcaatga gtaaatctaa aaaaagaaca gtactttgga tctcagacaa tcttttctca
     8821 ggaattgttt accattcaac acaaatgaat gtgcagctag tagccacata tgaggacggt
     8881 agccccgttc aattttcagg agatacattt atcagtttta actctttgaa ccctgcagga
     8941 ggaaaaagca cggatcttaa aggagaatat gcccactatg acaaaatgaa tacgacagat
     9001 tggtatgtca gaaaagatac ggttctttca gaatttaaat ctttctacaa caatctgaac
     9061 gtggtaggtg gccacccagg aggaagctct aagctgacac aagcggataa tgatttcaat
     9121 aatttgcatg ataaattagg agatccgaag tttggtcaag gtactgtgtc ctttaaaata
     9181 tctgaagcaa atccgacgtt tgtgattggt tcttctaatg ttcaaacctg gtttacttta
     9241 tcttctgcga caatctttag tgttgttcct gatcaacctg aaaaaacagg ggttgataaa
     9301 aatggaaata acgtgaacga taaaatgttg caagtaggag acacgattca atatcgaatc
     9361 aaacagaagg tgaatcgatt aggagtggat ttactagcgg tttacgatcg atttgagtta
     9421 atcgataatt taccaaaaga agtaaattac gtcgatgcac acgttgaaag tggtacaaat
     9481 aaaaaattcg atgtatctgg tgaagtgacc tatgacaaaa caaaacacca ggtaaaatat
     9541 gcagcgaaag ctgatacatt aaaaaatcga atgaagtata acggcgaaac ttacgaatta
     9601 gtgatcaatg taaaagtcaa tgaattggcc aatcaaaata gtgtagccaa aaatcaggga
     9661 acgtcgatca ttaataaagt agaaaaggat acaaatatca ttaccgttta tttccctaaa
     9721 attccagtga aagaagtaca acaaaatgga aaagatgtaa acggacgaaa tgacggtaag
     9781 aagggtacgc cgacagctcc tttaaacgca ggatctgaag tacaatatct agtcacacaa
     9841 aaatggcaca caaaaggcgt agacgctgcg tcagatcact acaaacaatt tagtattcaa
     9901 gatccaattg aagctcgttt gacatataaa gaaggttctg cgcaagtaat agataagagt
     9961 aaaggaaaag atattacttc tgaaggcaca ctaacctatg atagcaattc aagaacgctg
    10021 aagtgggaag cttccgctga cttcttaaat aacaatcttt tagatggacg agaaattcag
    10081 ctgatattta cagctaaaac tccattacaa tcagaaaaaa atattgataa ccaagccgta
    10141 gtagcagtag ataatgtttc taataaaaca aatgttgtga caattggggt tgatcctaac
    10201 ttgccacaag ttattgttcc taaaacaggt tctacacatt tagtaacgat tttagcggtc
    10261 agcttactat tacttgtgtt agcaactttt ggctacgcag ttctgaagtt caattaaaca
    10321 aaaaaacaag gctgaaaatc agccttgttt ttctatttga aaatgggatt ggcactaata
    10381 aatacgttgt ctgaagaaag tgatccgtct gcagaaactt cataagcatg ggataaacca
    10441 acgatacttt gattgttgta aagcaagttt gcgttatgag cagcgctatc tttccacata
    10501 accatagcat ttactgctcc tgtatctgaa aggatctcgt caacgtaaag gtagaaacta
    10561 ttgatatttt ctcctttagc accgtagcag cctaagcttt ctaagagatc ttggaaagta
    10621 ccgccgccag gtcggtaatg actgaagctt acttgtaatt cttttgcacg aatatctgca
    10681 gcttgttgaa cgatatcatt gaattgcatt ggggcaacgc cattttgttg acggtagcta
    10741 tttaaccaag caagttcttt ttgacgcaca tagttgttta aatcacttag tgagatattt
    10801 ttgtatttga aagtgatcgt tcgattttgg tcaacattat tgattacacg ttgtccattt
    10861 ttatcaacgg taacagatga atcgtcattt ctacacaaga agtagttttg atcggtcata
    10921 tttttccaag aagtagagaa actatctccc tcgtaaaccg tttgtgtttc agtagataaa
    10981 acagcgtttg tgtcaacgtt tatatggttc acagtgacgt tgtattgatg agtataggca
    11041 aaattgattc cttgattact tgtaacgttg tttaaagtca cagtgttgct agagctttct
    11101 ctaggatcaa gaacaaaatt agtgaacata ggtgcttcaa ttgtgatgct ttgtccataa
    11161 acgacgggat aactcttact gtaattatag agttcttgga tatttggatc agtatccgag
    11221 actttatttg gacgagcttt cacttcaact agaaagactt catcatagta aaaagtaaat
    11281 gtggtatttg tgttaatcgt ttgtgtttgt gtttcattcc catttacttg atactgtggt
    11341 gcttcaaagt tagtcaagtc ggttctagct tttgcagtgt aacttgtacc agcttttact
    11401 gagacatgtt cttcttttag cagcgttcca gtttgtcgat tcatatgacg gacagttacc
    11461 gcatattctg aaggtttagg tttgtgttgt aaccctttaa atgcagaatc aagactattt
    11521 ttagcattat ttacttgggt ttgagaagct ttagaatcgt ctagtacttt tttagcattg
    11581 gataaagcag tctgaaaatt attccaagat ccttctgtaa aatctccttt agcagttgat
    11641 tttacttggt tgtacaaggc ttgtaattcc gttttgttca cagtaggagc tggttttttc
    11701 tttaatccct tgtaagcaga atctaatgtg tttttggcac tatccacttg tttttgagtc
    11761 gcttttgagt catttaacac ttttttagca ttgtttaaag cggtttggaa tgcgttccag
    11821 gtattgtcgg tatagtcacc ttttttagtt cccttaacgc tcgtataaag agcttctaaa
    11881 gctgatttat tcactttggg tttagaggtg tcagcaccat accaaccaat accttcgtat
    11941 ttccagccat agtctttggt taaccattgt acttctttcc aacttgcagt gtagtgatgc
    12001 gaaccagagg taacacctgg aaggaaaagg cggtagatcg gtttattatt tttttcggca
    12061 gaacgccagg caggcccttc ataagtccaa ccgtaatttt tggtcaacca tttcacttca
    12121 tcccaactag ctgtgtaatg gtgatcacga aggcctttat tgtataaacg atagacaagg
    12181 tggccaccgc tagtcggtgc ttcccaagcg gggccttcat aattacccca cttaattttc
    12241 actaggtgat cacgttcttt tgtgctagag gtgtaaaaat gttcatggtt atttggatta
    12301 taaaggcgat acatgacgtt agttgaagca gcgtcagcag atccagcaag agcaactgtg
    12361 gcagcaaatg ttccaatagc tataacagtt tttgggttta ataatttcat gttttcaact
    12421 ccctattaaa tgattaaata ttttgtttac attcataata taccacggta taccgaggta
    12481 gtcaacggta taccgtggaa attttgaaat aaaaaagccg ccctatgaag agtgtaagga
    12541 tggctgttta tatgctatga agaaatggat tcggcgtatt tggttagttt ggcagcatta
    12601 tcaaatcgca ttttttcaat cgcacttttt ttctttatta aatccgcaat tgttgattct
    12661 gcgactgaag aatgtttgct aatttcatat gctgtcttgc cagtttcaaa tagtaaccat
    12721 tcgattttct ctttatcagc gtacattgca atgctccctc ttgatttttt ctttattata
    12781 gctctttttt gtaataaatc acaatatcaa gaggaaaagt ttctatagaa aggtgggaaa
    12841 ttatgagtat ccgttccccg tgtatcaaaa tgaggtttgt tgctactttc gttatttaat
    12901 atactaaaat aaaacaacga aagaaggaat agaatgaaaa agttaataat ggtatttact
    12961 tcgttgattg ccatgattgt tattttaggt ggatgtggta gtgaaaaaaa taagcaagat
    13021 agtcaagata atggcgtgtt aaaaccagga acagtgtgga aagaagaagt aggcggactt
    13081 gttcataatt taaaaattat agatgataca acatgggaat attccgaaag tgtttggcat
    13141 cctgaacaag tacaaataac agttgagaag ctaaaagact ataaaggatt agaaagatat
    13201 aaggtaatcg attcaggtgg agtagattta ttcataggta aaacagcctt aatagttccc
    13261 tataacaaag acggtttaga aacgtttaaa tttattccaa caaacgaaaa agaacatcga
    13321 gataaaagtg aaatattaaa agaatacagt actaaatctg gatacaagtt acaaaagact
    13381 agcgagtgat tgctagtctt tttatttatc ctgaaggagg aatcgcagtg gcaaaagcta
    13441 aaacgccatt tgaaaaagcc gcgtctaaaa tcttagaacg tcgcggtgta tctgaaacac
    13501 agtggaaaca agaagtcctt gaaaaagcac aatttgacct attacgggga aaagacaagg
    13561 acttagaagg atatatctat caacgtgaac gtgaaaagct tgttttcgat gaaattatta
    13621 aacactaaaa ggagcgaaca attttatgtt tgaatcaatt acaatgaatt ttttaacaca
    13681 accgaaagta gtagctgcgg ttgatatttt tagagcatta caaggtgcag caactagttt
    13741 aaaaaacaat ctgatgattt ttgcggctgt agctgccgtt gcctccattg ttcttggggg
    13801 gattttcttc ttctttggcc gaagaggagc tgaaaacggg aagatgcacg taggttatgt
    13861 attgatgggg gtaggagtta tattagctgc cgcctctatt gtggtttcgt tatttggaat
    13921 gtttggacaa accccacagg gactataaaa aataaataga aggagtaaaa agtacgtggc
    13981 tactttttac tcctttttta tgaaaggaga atactcatgt actttacgat ttcaggcaat
    14041 accaaaaaag ataaagagtt aataaaaaaa gcaacgaaga aagtgggaaa gcgtactgcc
    14101 tttttaccga aaacaattaa atattctgtc attgaaacgg aagaacagct acatgtgtta
    14161 ggacaagtgt tagaacaaga gaatcaatct tctgttaaat tgactctcac gttacatgat
    14221 aaagaaggaa aaatagaaaa ggctggcaca gaatacgttt ttggcccact ggattatcaa
    14281 atttcagaag gtttttatcc tacattgatg gatttgatcc gccttgatgt gtttaaggaa
    14341 acggatgaaa ccatgagtgt ggaagagtct tatcgaaacg aaaaactgct acaagtttta
    14401 gaagaagagt tagagagcga ccaaagaaaa gtgagtcaag tgagtgaagt cgaagtaaat
    14461 gatgaagtac catgggaacg accaatcttt ccaaagaagg agtcaaaaga aaaagagaca
    14521 gtctatgttg acgaacaagc tgatattccg actcagaaat cgatatttga ggaagagaac
    14581 gatgtaatgg gtcaattgga ggtagatccc tttgaagaaa caatggagga tgaagagacg
    14641 gccgaagtag aagttgatct agttgaagct aatgaagagc caacatggac tgaaggggat
    14701 tctcaggaat atattgaacc acttatcagt gaagaagtag agaaaaacga aacagagata
    14761 caaaaggcaa ctgtatttga ttatattttg gatcaatatc cgccagagca tgaatggatg
    14821 aaagaaaaaa ataaattatt tttaaatgag ctttattcga aatatcaatt accccaaacc
    14881 tatcaagcat ttttagagag taaagaagaa ttaatctcac agggtaaagg cacactttta
    14941 gaacagttag agtctgttca atcaacaaat tggcatgagc ttgcacaaga agatttatta
    15001 gccacttttg aagaacgtca acaagaaaca gatcaagaaa taaatgagta tgcgacggaa
    15061 cagtcatcta attgggagag aacaaaagag gaactaaata aagaagaaca acgagtgatt
    15121 gaagaagaag tacaaaaaat taaagagcga tacgaaaaga aacgcgacaa tgcgtattcc
    15181 ttgtgtatag agaaaattaa taactttact aaaactaatc aagaagcttt atcgaaagaa
    15241 aaagaaaact tgttaaaaga acgtataaaa gaacgaaaag aagagttcta ccgaggttta
    15301 cgtacagata agctaaagat tagcaaagaa ttaaatgaac aactgaataa actttatcaa
    15361 acaacgactg aagttcttat ggaaagacac gggcagatcc agcaagaatt acaagcacaa
    15421 attccaaaat ggaaaaaaga gtatgaaatt caacaacaag aattgaaaga acaagaagaa
    15481 aaacaacgac aagaagaaaa agagcgcgaa caattagaac taaaaaaacg tgagcagcat
    15541 cttgcagaac aaaaagtggc gatagaaaaa caacggttag agcaagaaaa ggaaaaagaa
    15601 gcagctgctc gtaaggaacg tcaagaacaa atggatacat tgcgacaagc acagttgaat
    15661 tatctagcac aacctcaacc agtagctgct gttcaaacgc cagtgaacaa ccaagcaaat
    15721 ttaactcctt caccacagcg ttcctttaac ggatggatgg tgggatgtat tgcaagttta
    15781 agcttgcttc taggtggagg aacagtattt gcttttaatc atatcaatca atcttcacag
    15841 gcagattctg tagccacaac ggcgtcaatc aaggaagaag cccaggaaca agcaagaaaa
    15901 gagtatgaac aacaattgga agaattattg aatcgtacca atcagtcaac agcagagtca
    15961 agtatggaaa atagcaaaga gagtgatcct agtaaggcta aagaaggatc aagcgattca
    16021 actgtacact caaacacttc cgaaagttca gaatcaaatg aggtgaaata atgcgtatta
    16081 gagaaacaac gagacgaaaa gacaaacgat tcaatttaga aaagaatagg tatctttctc
    16141 atttctatcg ctatactcct tcaggagaag agctaccaga aaaacatgac aaacgtattt
    16201 ctcaaaagat tttttatagt ggcttagctt atcttccttt aaactatgtt gttacaagaa
    16261 taccagaagc atttcatcga tttgagatta ttccaggaag tttacaagga gtgaatattt
    16321 ttcagttaca agaaatgccg cagttattcc attttgattc aactggtttt tggaatcctt
    16381 tgaccccaac aacaagttta ggcacacgtt tagttgtttc tacgatttgt gttgttccag
    16441 ctgtacttat gggattgaaa atggacatgt ggtttcgtcc gctagcagat gggcaaaaag
    16501 gagataaccg attaaaa
//
LOCUS       DACEPW010000047        16147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEPW010000047 DACEPW010000000
VERSION     DACEPW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.048"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(207..626)
                     /locus_tag="IW819_001214"
     CDS             complement(207..626)
                     /locus_tag="IW819_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1244329.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(628..1212)
                     /locus_tag="IW819_001215"
     CDS             complement(628..1212)
                     /locus_tag="IW819_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1244330.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLNEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(1259..2734)
                     /locus_tag="IW819_001216"
     CDS             complement(1259..2734)
                     /locus_tag="IW819_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1244331.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDVIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVKDWEKYLK"
     gene            2915..3226
                     /locus_tag="IW819_001217"
     CDS             2915..3226
                     /locus_tag="IW819_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244332.1"
                     /translation="MKKMEESFPKAAKVENIANILKVTFENGEVKYVKSHWTEEITDD
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3341..5677)
                     /locus_tag="IW819_001218"
     CDS             complement(3341..5677)
                     /locus_tag="IW819_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1244333.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSD
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            5819..6487
                     /locus_tag="IW819_001219"
     CDS             5819..6487
                     /locus_tag="IW819_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1244334.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     HIELIITDVMMPEMDGYDLVGSLRNARFDMPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSILTIGQTKLELDTYTVVQNQHSFILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6490..7530
                     /locus_tag="IW819_001220"
     CDS             6490..7530
                     /locus_tag="IW819_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1244335.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRVIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQVNGKIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAIIRLSK"
     gene            complement(7770..8651)
                     /locus_tag="IW819_001221"
     CDS             complement(7770..8651)
                     /locus_tag="IW819_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1244336.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITDFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            8810..9538
                     /locus_tag="IW819_001222"
     CDS             8810..9538
                     /locus_tag="IW819_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1244337.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            9695..10012
                     /locus_tag="IW819_001223"
     CDS             9695..10012
                     /locus_tag="IW819_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1244338.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10005..10613
                     /locus_tag="IW819_001224"
     CDS             10005..10613
                     /locus_tag="IW819_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1244339.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            10668..11459
                     /locus_tag="IW819_001225"
     CDS             10668..11459
                     /locus_tag="IW819_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HAQ1244340.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADYELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            11597..12085
                     /locus_tag="IW819_001226"
     CDS             11597..12085
                     /locus_tag="IW819_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1244341.1"
                     /translation="MKAEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12136..13008
                     /locus_tag="IW819_001227"
     CDS             12136..13008
                     /locus_tag="IW819_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1244342.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13184..13546
                     /locus_tag="IW819_001228"
     CDS             13184..13546
                     /locus_tag="IW819_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1244343.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13555..14307
                     /locus_tag="IW819_001229"
     CDS             13555..14307
                     /locus_tag="IW819_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244344.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14321..16123
                     /locus_tag="IW819_001230"
     CDS             14321..16123
                     /locus_tag="IW819_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1244345.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQTTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
BASE COUNT         5038 a         2814 c         3297 g         4998 t
ORIGIN      
        1 caaatttatg caaaaatgtg gaaacccatg tttacaaaat ttttcttact actcgaaacg
       61 aagcggcttt atcacaacct cttgataaac ggcgttcaat cagctgtccc tcggtattag
      121 ctcaaaaacg gcaaaatatg agaagctatt tttccatttt tttctctcat actcaggtca
      181 taacgctgat ttcacaacct cttctttcat aatggttttt caccatcttt ataataaact
      241 ggttctttga tatggaaatc tattttttcc aaccaatcta aaatcttgta acctaactct
      301 tctgacatcg gaaaagaggc gttgatttgt gcattggaga tatggtcaac atggataccg
      361 gaagtaatcg tacaacttcc cggaacggca gttttgatga tcacagctat tttttcggct
      421 aagacatcgt ctttatggaa ccgaccatcg tggctaggga aacgtacagt ttgcatctct
      481 gtatcacgag aaaatgtagt gactgttccg atatgtggtg tatctccgcc tgttaaagtg
      541 atcaataagt ctttaccgat tactttaaca gcagcttgga tcgtgaaacc tagtttggtt
      601 acttcaacta tcgtttcttt ttccatatta atcaccctgc caacgttttg aatgctcatt
      661 tacaattcca aattgatcca aaagtttcat tgtatggtga ttaacaagat cttggatcgt
      721 ttgaggctga ttataaaaag caggaattgg tggtatgatt tgcacaccta gtcgtgctaa
      781 tttagtcaag ttttccaagt gaatggcatt taatggcgtt tcacgaggaa caatgactaa
      841 ctttcgttgc tccttcattg ttacatcagc agcacgtgca ataagattgt cgcctaaacc
      901 aacagagata cctgcgactg ttttcatact tgctggtacg atgatcattc catcggtcaa
      961 aaatgatccg ctggcgacag cagcacctaa atctttgttt gaataatggt aatcaaacag
     1021 gctttcgaat tcatttagtg ataagtcgga ttccagcttt aagttctgtt ttgcccatgg
     1081 actgatgatt ccgtgtgttt ctatcatcgg atattctttt agtttcttca ctaggttgat
     1141 ggcataaatg gtgcctgaag cgccacttac accaatcacg atttttttct tccgttgtgc
     1201 caattgttcc attttccgca ctccttttta tgatgtcaaa acagttgcag atcattcatt
     1261 atttcaaata tttttcccaa tcttttactt cttggaattt tgcacgtaca aaatgttctt
     1321 tcatatcgaa aggaactgta ccgtcaaaga tagtttttgc actcatccca cggaaacgaa
     1381 tagagccagg attatattct ggtaattctg atgggtccaa tggatggttt ctcattcctg
     1441 ggatcacaat gacgtcttta tctccttgga aacgagtgtt gaaagtccaa actacatcgt
     1501 tcatgtcgaa aatatcaaca tcttcatcga ctaaaatgac tgtcttcaat tctttgaagg
     1561 ctgataaagc aagaatagct gcttgacgct gaataccttc atccgcttcg ttttctttac
     1621 ggatttgcat gatggtcatc aatttaccac cacctgcagg aggattgtag acatttacta
     1681 cttttcctgg aatagcttta tcgaccaagg caagaatact tgcttctgta ggaataccgg
     1741 ccatagatac gtgttcttca ctgggaccaa tcgttgtttg catgattgga ttgtcttttc
     1801 ggtgagtaac tgctttgact ttcactactt gtacagctgg attagcatcc ccagtatagc
     1861 caggaaattc tggcattgct tttcctgagt tcgtgttgat atcttcttgc attgtttcat
     1921 ttggcatgat ttctgcttca atgatgaatt cagcacgagc aatacccact tcatcgactg
     1981 ctataccatc aacgatttga ataggttcgt tacgcaaagc tcctgcacac catagttcat
     2041 tgtagccaag tggagtggta ggtggttcga aagtcgtgcc aatggcaatt gctggatcta
     2101 aaccaatatt gatagtgata ggcattggtt tattcaaggc ttcgaattgt ttttggaagt
     2161 gaccaatgtg gcgtccgcca ggcatgatgt acataccgat cgtatcttta tcttctagaa
     2221 ccatacgatg gatcgttaca tcgctcattg ttttttctgg atctgatcct aagactacac
     2281 ccattgtgat gtatggacca gcatcttcag gagtatttgt aggtgcaggc aaaatgttgc
     2341 gaatatcgaa gtctgcatcg gtagctagat gaacgacttc ttgtgctgga gcgtctgctt
     2401 tgtctacttt cacaggtttg attggatttt ctacagcgtc tttcaagaaa cgaccaagtg
     2461 ttcgataatc atggtgcaaa atttcaccta cacgtttacg actagccatt gttccgatgt
     2521 tcacacgtgt gccggggaaa cctttgatat tgttgaaagt catagctggt ccttcttgag
     2581 tcggacgttt tactgtaccg ccagcaccga tataacggta aacaccagat aattctgctt
     2641 caggatcgat ttcaatatct gtttcgtgat attgacctgg aagtgttttt agttcttcga
     2701 tgacttttct taaatcatac ggttgatctg tcatgagaga ttcctcctta gttgtcttta
     2761 cctatctata gtaaaaggtg aaaaagagga aaacaattca acttgttcgg taaagtattc
     2821 tacaatggaa tggatgcgtt tttttgataa tagaataaac tatgatatac ttatcaataa
     2881 aagaaagcgc atacttagtg ttggaggaag gaaagtgaaa aaaatggagg agtcatttcc
     2941 taaagcagca aaagtggaaa atatagcgaa tattttgaaa gttacatttg aaaacggaga
     3001 agtaaaatac gtgaaatctc attggacaga agagataacc gatgacttgc aatttggaaa
     3061 aaaaggaaga ggaaaaagaa aaaatcttct cgcactttct cgtaatatgt ggattgggac
     3121 tgaagtaacg attgaagcag atggaacagt attcataaat ggaaaagaca ggtatacgcc
     3181 agaagagttg tggtacaaag gaaagaaaag tataccagaa ttataaaaaa taattaaaaa
     3241 aaacagcgcg aaacaaggaa catattactt cctcgtttct tgctgttttc tactatttca
     3301 gcgctcttat gactcaaatc taaatgtggt tgtcacaacc ttattctgca cgcaaagcga
     3361 tagcggcgtc ttttttcgct gccattcgtg caggaagatg accgccaagc atcgtcaata
     3421 tagtagagac gatcactaga ataatcgcat ggacaggatt tagttgagca acatttttta
     3481 gatccgtcat gttgtataag atgctattga tagggaatgt agctagccat gcaataaaca
     3541 cacctaatat cccagaagaa atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga
     3601 tgtctttttt acgtgcacct aaagctttta gtacaccgat ttcttttgtc cgttcaatca
     3661 cagatgtata agtaatgata ctgatcatga tcatacttgt tactaaagag atccctgcaa
     3721 aagcaataag gacataagtg atagcatcca tcaatccgcc agtcagttca gtcatcgttc
     3781 cagctaaatc agtataaatg attttatctt catctgattt gcctttattg taatcatcta
     3841 gataatccaa gatcttttct ttgtcttcaa aattgttcgg ataaatcatg atacttgaag
     3901 gcaagctgtc tccacctaaa taactaagca atgtttgttt ggcattttcg tcaacttttt
     3961 cagtagttaa gacatttaca tcactgtctc tttgagcttt gacgatttct gattctttgt
     4021 tttcgttgac gatttgagtc gttaattgat cgctataagc gattcctgga gataagagat
     4081 tcattgtaga agaagatttc acgcgtagga tccccgagat cttcaattca tcagaagcat
     4141 tctgatacat cgcatcataa tctgtattag ggataaaatt cccagttgga agttttgtat
     4201 agtaagtatt gttgtttact aatttgaatg ttgtaccaac gatatcatca aaatcaagtt
     4261 tttcgtcttc tttcacatca aatccaagat tcttcaatgc gttgatatta gtattattat
     4321 tgccgtctac gatcagcacg acatctgttg ctgaggctgg ataacttccg gcaagtaaag
     4381 aataattgtc ttttaaaaag ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca
     4441 ctcccacacc tgtcattgct gacatcgtac tagataaaga aagagattcc gtatcaggat
     4501 tttgattcga gaagctgaca ggctgtactt tgccatttac atcacgtaaa agattgattc
     4561 ctgtagtacg ggtaaatccg atattattac ttaagttagg atcgatgtct gttacgtaat
     4621 cgacgtatgt ttgatcaatc ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg
     4681 cagtaatcgt tttgctgtct ggataatccg ctttgcttgc acccaatccg gcatcgtcac
     4741 gagtctggtt agtcgttacc ttagaaatag taatcggaaa tttcgccatc gtttcagctt
     4801 gcgtgttatc gatttgtttt tggaatccag aagaaaggga cagaacgatc gcgattccga
     4861 tgatcccaat actggaagca aaggaggtca agaaagtacg tccttttttt gttcggatat
     4921 tgttgaagga aagcttcaac gctgtccaaa aactcatctt tgttctgcgt aagttaaatt
     4981 gatcatcttt tggccgttcg atgtgaggat gggaatctgt taagatctta ccatcagaaa
     5041 attcaatgat ccgatctgcg tattgatggg ccagttcagg attatgtgtg accatgatca
     5101 ctaatttttc tttcgataac tcttggatca attccatgat ctggatactt gtttctgtat
     5161 ctaacgctcc ggttggttca tcgcacaaaa ggatatctgg gtcattggct agtgctcgag
     5221 cgatggccac tcgctgcatt tgtccaccag ataactgatt tggcttttta tgcatatgat
     5281 ctgtcaagcc gacccggtgc aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa
     5341 caccacttaa tgtcatccca agctcaacgt tttcaataat gcctagatga ccaatcaagt
     5401 tgtaactttg aaaaatgaaa ccgatagagt tgtttcggta ggcatcccag tcactgtcct
     5461 taaagtcttt agtagacttt ccgttgatta ccatatcacc ggaatcataa ttatctagtc
     5521 caccaatcac attcagcata gtcgttttac cagaaccact aggacctaaa atcgctacga
     5581 attctttttg tctgaaagcg acagacacac catccaatgc tttggtggtg gtttcgccga
     5641 ctttataata tttttttata tcttttaatt gaagcataat cacccttctc ccgtattgaa
     5701 atattcggta tactaataag tatatcagaa caatgatcgt agtatacgaa ataaatgtta
     5761 actgaagatg aactacaaga aaaaatctag aaatttttca aaaaacagag ggagatcaat
     5821 gaatcgtatt ttagtagtag aagatgatcc gcaattacag ttgctttacc gttctgtgtt
     5881 ggaacgagcg ggcttttcag tcatcaccgc actgaatgga gaggatgcgc tgaaaaaact
     5941 agaagcgaca catatagaat tgatcattac agatgtcatg atgcctgaaa tggatggcta
     6001 tgacttggta ggatcattac gtaacgccag attcgacatg ccagttctga tgattacagc
     6061 aaaggctgat ttcgaggaca agaaacgtgg attcaaatta ggtgcagatg attatatgac
     6121 aaaacccatc gatgtcaatg aaatgatcct gcgtgtggaa gcattgcttc gacgttcaaa
     6181 aatcaattac agcagtatct tgacgattgg gcaaacaaaa ttagagctgg atacttatac
     6241 agtggtacaa aatcagcatt cttttattct gcctcaaaaa gaatttctgc ttctgtataa
     6301 attactgtct tatccaaaca agattttcac tcgccagcag ttgatggacg atatttgggg
     6361 attagacacg aatacagaag aacgaacgat cgatgtacat atcaaacgtt taaggacccg
     6421 ttttgaaaag acagaagatt ttcaaatcat cacagtacgg ggactaggct ataaggcggt
     6481 tatttaaaga tgaaaaagaa gctattttca caattatgga tctattttgc tagtatcgta
     6541 tttatttcga tcttagtaac actgctgtgt tttcttggtt ttattttctt attgtctcga
     6601 cattctatct cgcctgctga atcggctgga aaaccaacgt tatacccgtt attcgtcttt
     6661 gcggggttca gtatggtcgt gggtacagga atatcgattt ttgttgggcg gcgtatcctg
     6721 catccaatca gtgcgctagg gacaaatatg agtctggtag caacgggaga tttttcgatt
     6781 cgaatggatg aacagcagaa agtagcagaa gtacagcaac tgtataaaga tttcaacgtt
     6841 atggtccaag aactcaatag tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc
     6901 catgaattca aaacaccact agcaacaatc caaggatatg ttcaattgtt acaagcccca
     6961 aatctttctg acgaagaacg ccaaatattt ttatatcggg tcatcgaaag tatcacgcag
     7021 ctttcacaat taacagagaa cattttaaaa ttaaacaaac tggaaaacca gcgtatccaa
     7081 ctggaaaaga aagagtatcg attagatgaa caaatcagag aagtaatcgt ttttttgcag
     7141 cctaaatggg aaaaagaaca gctagaattg gatatcgaac ttgctgcagt aaactataca
     7201 ggtaatgaag agtttttgta ccaagtgtgg ctgaatatca tggataacgc cataaagtac
     7261 aatcaagtca atggaaagat tcatatcaaa ttatttgaga cagccacaga gatcgtactg
     7321 gaagtgaccg attcaggtgt aggaatgaat gaagagaccc gagatcgaat gtttgaaaaa
     7381 ttttatcaag gagatacaag ccgtcagatt tctgggaatg gactagggct ttcactggtc
     7441 aaaaaaattc tagaactaca tgatggacgt atcgattata gcagtatcga aggagtaggg
     7501 acgacagcaa taatacgttt aagcaaataa taggaaaaaa gaacaaaata cctaaaaatt
     7561 ctagtgaagg tttttaggga tttttagacg tttttttgtg attagcgaat tgctcttaat
     7621 gttcttccgg cataaaatcg ctgatcttct attgaaatac gagaaacgtt agatgtgtca
     7681 ttgaatcaat ggcacgatgt aagaaagaga gcgaaagtca tgcgtcgctc tcttttttgg
     7741 ggtggataga tggacagaaa atgtagggct tagttttctt cttgatttaa atactcccaa
     7801 aatgtatctg aagccaaaga atgctcaccg actttacgag taaatgcaag ataactctct
     7861 gttgttggag aaatagggag aatcacagta ttttctttta atagattctt gactgttttt
     7921 tccataacaa tagcaatccc catgtcattt gcaatcatat tgatgatcaa gtcgattctt
     7981 gctcctttat aagagatttt tggttcaaat ccagcttcgc gacacagatc tagtacaggt
     8041 tgatacaagt tcgtagtggg gccaagtagc aaaaagttct cctttttcag ttcgcttaga
     8101 tccaatttct ttttactagc taaaggatga tttttaggta gaactgctac aaaatcgtct
     8161 gtttcagtag gcaaccactc aatcgaactc ggcggggcct ggaaacttct agcaaagtgg
     8221 atcgtgtcac tatgttcatt ggcgtcattg taagttaatt cgacactttc cacttctttc
     8281 aactggactg taaattcagg atggcgtttc aaaaagtcgg tgattttgga aaaaccttta
     8341 taattgatca ttgtcggtat cgttaagata tttaatgaaa gattgttgtc tttcgtatag
     8401 gtttcaagca cttcttgcat agcatggtat tgttctacga cattttttac atatgggagc
     8461 aataagcttc cagcttcggt caattcgatt tttctatggg cgcgttcgaa taatttgacg
     8521 tctaattctg tttctaacga aagaatctgt ttagaaatat ttccttgggt ggtgaattgt
     8581 cgatcagcag ttttggtgaa attcattgtt tcggctaaat caagaaaaac ttcgaattta
     8641 tggatatcca tcatattcct cctacttatt ccaataaaga atactttaac acaaaaaatg
     8701 aattggtgta atcttctctg tctgttacaa tctttcttgc agcgaaagca ataattacag
     8761 aaatttttct gatttttttc gcttcgtaga aaaagagagg agttttttga tgccaactgg
     8821 agttttgatc aatgtaggtt ccgttttgct tggcggacta attggcggat tagtaggaaa
     8881 taaattaagt gaacatttta aagcacagct gacgatggtt tttggtgtgt gttcgatggg
     8941 gatgggaatc tattccattg cgccgatgaa aaatatgccg gctgttattt ttgctttagt
     9001 gataggaaca gcgattggtc tgattgttca tttaggtaat gggataaata agggggctgc
     9061 gctgatgcaa gtacctattt ctaaaatctt ccctagtgaa aagcttggta tgacccacga
     9121 tgaatttatc agtacattag tgacggtcat tgttctattt tgtgcaagtg gtacggggat
     9181 ctatggaagt ttagattctg gaatgacagg agacagtacg attttgattt ctaaatctgt
     9241 ccttgatttt ttcacagcag ctatttttgc gtgtaattta gggtatgtcg tttctgtgat
     9301 agcaattccc caattcatta ttttttatat tttgttctta ttggcaaaat ttatttatcc
     9361 attgactaca ccagacatga tcttagattt taaagcttgc ggcggattct tgatggtcgc
     9421 taccggtttt agaatgatca acgtgaaaat gttcccagta gctgacatga tacctgcaat
     9481 gatcgttatc atgccactga gctggatgtg gacaaattgg attatgccac tgttataata
     9541 aaaatcaaag agaagccatt tttttgctga ctaaaaagga tcaaatcttt tagtcagttt
     9601 ttttgtattt gacaaactga ttttgaaact atattatatg ttgtataata tagattgaaa
     9661 taaaatatta cacttgagaa tagaaggtga cgtaatgcac atccagattc caactgttct
     9721 tcttgatggt actgtgttag cagcattaag caaggaagat atgtatgggt atgccttaac
     9781 aaaaatcgta caagaaagtc tctctgtcag tgaatctaca ctatatccgg ttttgagaag
     9841 attgaaaaaa gaaggatatc ttgagaccta tgatgaacct tttgaaggca gaaaccgtcg
     9901 atattactcg ttgacaccaa tgggaagtcg tcatttagaa gaaattattg atgaatggat
     9961 tgtttttcga gattctttag accggttatt ggaggagagc acaaatgaat gaatatttga
    10021 atgaagttgt ggatcatctt caagaaattc ctgaagacga tcgttttgat ttgatccagt
    10081 attatgagga atatttttta gattctggaa aaacattaga tcagatcatt gatgaatatg
    10141 gtacaccaaa gcagtttgcc ttaaaattaa aaatcagtta cttttcagaa gaagaacaag
    10201 cagaaaacga gttagtgaaa aatccaaaaa gacaaattcg cctgatttgg ttaatcatta
    10261 tcggtttatt tgcttctcct gtcctcattc cactagcact ggggtttata ttggttattg
    10321 ggggactatt ggttgcttta ctagcgctca tttttagtat ctatgtttta tgtatcagta
    10381 tcttaggtgt tggcctattc atgatcatat caggaataag tgtgtttgga caatcgattc
    10441 tgagtggatt attctttatc ggtgtcggtc tgttcgccac aggaagcgca gtatttattt
    10501 cgccactgtt gctgaaagga acaaaagggc ttttttatgt catgatgaac tttgttaaat
    10561 ggatgggacg acgttttatt acaaaaagag atctgcattc agcgggcgtt tgaaggagga
    10621 cacataaatt gagattgaaa cattttttga ttaccggtat tggtctgatg cttactggtg
    10681 gcctcctagc aggtgcaact tatacattag gcgctcaaaa gagtctgact tgggataatg
    10741 gtcctcggtt gatcgatatg gttactgaga caaaaaaaat caaggatgca aacaaaatcg
    10801 tcatccatgc agaaaatcaa acagtttcgg tcatacgcgg attggatttt gagataaaaa
    10861 cgacttatga taagacagaa aagccagaaa taaaggagaa aaatggcgtg ttgactgtga
    10921 atgcagtcaa aaaatcaaca ggtgcaatca ttagtttaga agacacaagt gcacaaatca
    10981 ctattgttgt ccctgcagat tatgaattat ctgaattgac aatcgaaggg aataatagtg
    11041 caattgatat caacgaagta tcaatcaaaa aagtgaatac taagacacat gatggctggg
    11101 tatcgttcaa tgaatttaca ggaggaacaa taactgcgaa agatgaatta ggctcacttc
    11161 gactagatga ggccaatgtg gataacttga tcgtcacagg tgaaaacagc aacttccatc
    11221 tagaagacag tttgatcagc aaagaaggaa aagtcatcct aaacggtggt aatttatctt
    11281 tgcatgagat ggaaaacagt ggctatacta tccaaacgtc tggtgaatct tatattgaaa
    11341 aagactacca gccaataaca aatactttgt tagaacaagg acagaaaaag ctaaagatca
    11401 ctgctaaaga cacatcgatc aatattacga cggatcaaga ggacaatgaa acagattgat
    11461 cctgtactca actagatatt ttaagaaaaa acagcaaaga tccttccaat ctttgctgtt
    11521 tttcatcgct taacgcacat aactatagta aaataagaaa gaaaatatct ataaagaaaa
    11581 cagggtgaaa aagtatatga aggcagaaga aggtcaaaga gaaaagttaa agacagaaat
    11641 ggaaagatta catacttata tcactcaact aagccaaacc ttctatgatc cagacaaaga
    11701 aaaagtgatg gttaattacc cgaacaattc agaaggtcgc caattagagc aagtgtacca
    11761 tgaagttttc aaacatttat tgacggtgaa gaaagagctg gattattatt ctttaccgat
    11821 tatcgataca ggaatattga agtatgatgg gaagaaggaa cgatttatct ttaaatctgt
    11881 tcgggaagat ttgcctcttt cagcagggat ggatctagag gtcttggtag aagactattt
    11941 tacagaagaa aagcactggg ttcgaacaaa attggattat ttaccgcagg cagccggagg
    12001 gacacaggca agcggttggt acatcactga agacaaagag ttagaactag aaggtgtaat
    12061 ggcgcgtatt cgaaaaaaaa attaaaaaag taacattaga tatttattga aggataaagg
    12121 agcgcaggaa gtagcatgag agaaaaaaga gcattttata tcaatggtta tgcagggatt
    12181 gtcggattgg tcgtattagc tttagttgga ttgtttttct tctatttagg catgtggcaa
    12241 gcgaaagttt gggcactttt tttaagtatt tttctttggt tgatcacgtt attgttgtta
    12301 agttcggcaa ctgtagtcag tcctaatcaa gcaaaagtaa ttttgttttt tggacaatat
    12361 ttaggtacga ttcgagaaaa tggctttttc cttacgatac ctttggcaca aaaaatgact
    12421 gtttcgttga aagttcggaa tttcaatagt tctgttttaa aagtcaatga tttggatgga
    12481 aaccccatcg aaatctcagc agtagtagtc tttaaagtcg ttgatacagc aaaggcatta
    12541 ttcgatgtag cgtattatca agattttgta gaaatccaaa gtgagacagc cattcggcat
    12601 atcgctagcc aatacccgta tgatacattc aatgagaatg atttgacgct tcgaggaaac
    12661 acgacggcag tgtctgacga gttgcaaaaa gaactgcaag agcgtttagc agtagcaggt
    12721 gtcgaagtaa tcgaaacacg cttgaaccat ttagcctatg caacagaaat cgctagtgcc
    12781 atgctccaac gtcaacaagc aaaagccatt ttgtctgctc gacaaaccat tgttgaagga
    12841 gctgtcacaa tcactcaaat ggccttagag caaatcgaag atggacaaga aatcaatttc
    12901 accgatgatc ggaaagtcca attgatcaat aatttacttg tctcaatcat tacagacaaa
    12961 ggaacgcaac ctgttatcaa tacaggcgac atgaaagacc gtaagtgaca tactttttgt
    13021 aagataggaa gaaggagttt aggacggatt attgtcttga actctttttt tagttgtttt
    13081 aaagaaaatg acaaaattga aagaagaatg atagttgatt cgaaggcaga attgttcgct
    13141 tttttctata atcagtatat aaagtaagag agaaggggaa aaagtgaaaa aacttctgat
    13201 cggtctactt gttttcatcg ttgtggtact tgcaggggtg aaaatcgcag attacgtagt
    13261 tatgggtgga gaagaatact atgtacaaat tacgacagat ggtacaaaaa cagtttcaaa
    13321 atcagactct ggacaagaaa cagtcaacta tagatatatc ctgcctggct atacgaaaga
    13381 gggtaaggaa aaagaactgg acttcaaggg acaattgact cgaccgcttc gtaaaggagc
    13441 atatttaaaa atcacctgga acaaaaataa aggtgtcact tcgtatgaag aagtcgaaaa
    13501 aaaagacgtg ccgaaaacag cactggaaaa actaattaaa gggtagggaa agaaatgaac
    13561 aaaatagtcg aagtacagaa tgtaacaaaa gtatacggaa aaaacaatga gaaaaagacg
    13621 caagcattaa gcggcatcag cttcgatgta gaaaaaggcg aatttattgg gatcatgggc
    13681 gcttctggtt ctggtaaatc gacacttttg aatattttat ccacattaga taaaccaaca
    13741 gatgggcata tccgaatcaa tcaaaaagat gtgaccacat taaaaggaaa tcaactagca
    13801 gattttcgtg cgaatgagat tggctttatt ttccaagact ttaatttatt agaaagcttg
    13861 acagcacaag aaaatatcgc tgttcctctt tctttacaag gcgtcagacc aaaagaaatc
    13921 aagcaacgtg tccaaaaaat tgcagaacgt ttatccatct ctcatatttt ggaaagctat
    13981 ccttctgaga tttccggtgg acaaaaacaa cgggtcgccg ccgcacgtgc attgatcaca
    14041 caaccaacga ttctattagg ggatgagcct acaggtgcgc tggattccaa aagtgcccgg
    14101 gatcttttag atacgatgga cgaattgaat acaaaagatc atgtatcgat tcttcttgtg
    14161 acgcatgatc cgttttctgc aagttattgc caacgtattt tattcatcaa ggatggcggt
    14221 atccatcaag aagtgaaacg tggagatcaa agccgtgatt ccttctatcg agaaatacta
    14281 tctattcttg gaaacttgga acagtagggg gagattgact atgctttgga aattatcact
    14341 tactgggatc aagagccgtt taagagacta cttggtgtta ttctcaggat tagttatggc
    14401 atctgctatt ttttacatgt ttgaatcaat ggcaagcaac gaagcttttt tgaaaagtaa
    14461 tacgatcgtt gcggctacag ttatcatttt ccgattcggt tctgttttac tagggatcat
    14521 cacattcgtt tatattttat atgcaaattc ctttttgatg acgatgcggc aaaaagacta
    14581 tgcaatgttt atgatgctag gagcaaaagg cagaaaaatc gcacagatga ttttttctga
    14641 aacattctta gtagggatcg cagctacttt agtgggttca gctgtcggtg taggattgac
    14701 aagtatcgtc caccgattat tagtggatca gttagatctc aatatttcgc attttacacc
    14761 ttttagtatt cacgggttgt tgatcactgt tttgtttttt gctgttttgt ttttattagc
    14821 tgctgttgtc aatgcttttt cgattgtcaa aaaaccgatt ttagcattga ttcgtgcgga
    14881 tcagacacca acaagaatca agcagcataa attcttgttt ttccttgaag tagtactggg
    14941 aattgctttt ctaggctgcg gttactatat gatgaaacac gtggggacct atcaagtctt
    15001 tggtatagga gtagccttag ttacgatcgt attaggaact tatttcattt tccattctgt
    15061 gatcattttc tttttatcat tactaaagaa atcagaacag atttcattga aaaaattgaa
    15121 caatttcaca ttgtcacagt taaactttcg tattcgtgaa tatacacaaa tgctctcaat
    15181 ggtcgctatg ctgtttgcct tggcactggg cgctttgacg gtaggattag gttttaaaaa
    15241 tgaaattgca gacttgacag aaaacaattc tggatatgat ctagtgatga ataatcctcg
    15301 ggcagaagat cagcaaaaaa tcaaagaact ttcaccaaca ctttctgctt cttatactca
    15361 aaaagaagat gcgggaacga tttactataa tgcaagtgaa tttgcggctc aaccattgat
    15421 catgataaaa catgaagatg gaacaccgcc gaagatcacg aaagaaaaag ttcctgctga
    15481 aaaaatgaat gaactgactg tacaagagga acttcgaagc tatgaattgc cggaacagca
    15541 agaaaaagaa atcaaggtag tcagtcaatc agaatttgat caactgaact tgccacaaac
    15601 cactttacag ctagttcagg taaaagactt ccaagcaaac ctgaaaccaa tcgaaacact
    15661 tgttgctcaa aataaaatga acaatccatc tttgcaaaaa gtagagtaca gcaaccagaa
    15721 atatgaaatg tatgaagtgt ataatggtct attctctggt tttgaattta tgggattctt
    15781 cctaggttta gctttcttga ccatgcttgc tagctgtttg atgttcaaga tcttgtcagg
    15841 aagcaagagt gacattgctc gttatgaaat gcttgaaaag ataggaacac gacgcgggtt
    15901 gcttaaacaa tcgatccgta gagaagtagg cgtgttattc ttagcaccag gaatcttagg
    15961 tgtagtccat gtcttattcg gattacaaat gtttggtcta ttcatgcaaa atccatacaa
    16021 agatatttgg gttccgttta cgatcttctt cgtattgtat ttcatctact atgtactgac
    16081 gacatggtta tatactggaa tcgtattgaa aaagagattg tgaaaaagct gtcctgcatc
    16141 aagtaat
//
LOCUS       DACEPW010000048        16132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEPW010000048 DACEPW010000000
VERSION     DACEPW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.049"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            181..2652
                     /locus_tag="IW819_001231"
     CDS             181..2652
                     /locus_tag="IW819_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1244346.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2755..4491)
                     /locus_tag="IW819_001232"
     CDS             complement(2755..4491)
                     /locus_tag="IW819_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1244347.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4488..5192)
                     /locus_tag="IW819_001233"
     CDS             complement(4488..5192)
                     /locus_tag="IW819_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1244348.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5323..5826
                     /locus_tag="IW819_001234"
     CDS             5323..5826
                     /locus_tag="IW819_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1244349.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5786..6124
                     /locus_tag="IW819_001235"
     CDS             5786..6124
                     /locus_tag="IW819_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1244350.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6145..7563
                     /gene="ffh"
                     /locus_tag="IW819_001236"
     CDS             6145..7563
                     /gene="ffh"
                     /locus_tag="IW819_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1244351.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7675..8253
                     /locus_tag="IW819_001237"
     CDS             7675..8253
                     /locus_tag="IW819_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1244352.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8257..8778
                     /locus_tag="IW819_001238"
     CDS             8257..8778
                     /locus_tag="IW819_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1244353.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8857..9411)
                     /locus_tag="IW819_001239"
     CDS             complement(8857..9411)
                     /locus_tag="IW819_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1244354.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9664..9939
                     /gene="rpsP"
                     /locus_tag="IW819_001240"
     CDS             9664..9939
                     /gene="rpsP"
                     /locus_tag="IW819_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1244355.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9951..10202
                     /locus_tag="IW819_001241"
     CDS             9951..10202
                     /locus_tag="IW819_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1244356.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10327..11118
                     /locus_tag="IW819_001242"
     CDS             10327..11118
                     /locus_tag="IW819_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244357.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11288..11809
                     /gene="rimM"
                     /locus_tag="IW819_001243"
     CDS             11288..11809
                     /gene="rimM"
                     /locus_tag="IW819_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1244358.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11799..12560
                     /gene="trmD"
                     /locus_tag="IW819_001244"
     CDS             11799..12560
                     /gene="trmD"
                     /locus_tag="IW819_001244"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1244359.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12696..13043
                     /gene="rplS"
                     /locus_tag="IW819_001245"
     CDS             12696..13043
                     /gene="rplS"
                     /locus_tag="IW819_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1244360.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13416..14078
                     /locus_tag="IW819_001246"
     CDS             13416..14078
                     /locus_tag="IW819_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244361.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKKKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14157..>16132
                     /gene="ecbA"
                     /locus_tag="IW819_001247"
     CDS             14157..>16132
                     /gene="ecbA"
                     /locus_tag="IW819_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family collagen-binding adhesin EcbA"
                     /protein_id="HAQ1244362.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDG"
BASE COUNT         5432 a         2926 c         3329 g         4445 t
ORIGIN      
        1 aacaccgttt attcgaaaaa aagagctacg acatagaact gtcgtagctc tttttttgct
       61 atatgacagg aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt
      121 ctatactgga taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac
      181 atgaagaata aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat
      241 aaagtcatcc gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa
      301 cgaatagaaa aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa
      361 atcgtattgg aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg
      421 ctttgtttag tagaatccta taagaacttg acttttttac cagttatcat agccaataca
      481 ggaatcgcca tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta
      541 aatacagcaa aagtcaatgt catacgggac gggatagaac aaacaataga tatagaagac
      601 cttgtaatag atgacattgt tattttagag actggacatc aaattccagc agatgcagag
      661 gtcgtggaag gaaaactgca agttaatgag gctttattga caggcgaagc agatgaaata
      721 accaaagaaa taggcgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac
      781 gcccgattag acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag
      841 acaatgggag aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg
      901 attgggatca tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat
      961 ggaaacacga tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct
     1021 gaaggactgt acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag
     1081 caaagagttc ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt
     1141 tgtgtagata aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa agtgatcgtc
     1201 tctaaaaaac aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat
     1261 gcaaaagcga tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa
     1321 accactgatc aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt
     1381 gtagcgttgg cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa
     1441 ttgccggaat atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc
     1501 tttggtgtgt acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta
     1561 ggctatattc tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc
     1621 aaaaaacagc aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat
     1681 gtagctgtcc aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca
     1741 gaagaagaat atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa
     1801 caaaagaaaa tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat
     1861 ggcgtgaatg atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga
     1921 aatgaagccg ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg
     1981 ccagaagttg ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg
     2041 tttttagtga agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg
     2101 acctatcctt tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca
     2161 tctttcttgc ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat
     2221 gtgatggaaa aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg
     2281 atctgcgggg tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt
     2341 ttgattgcag tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga
     2401 agtcagatca ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag
     2461 ctgttttcaa tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc
     2521 tcggcagatt cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa
     2581 aatgaaaaag agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa
     2641 cgagaaaatt gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc
     2701 tttcgaagca ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat
     2761 tttcggaatg gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt
     2821 accattaagg agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga
     2881 atgtcggctt cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc
     2941 aatgtcgata cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata
     3001 gcggataata atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt
     3061 gataattgga taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg
     3121 aatcgtcaac cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata
     3181 aggttctgta taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat
     3241 gatttcccga atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga
     3301 agtcagagtt tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag
     3361 tgaagtcaca ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt
     3421 ttctaaacgt cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc
     3481 tgaattagta aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat
     3541 ctcctgatgg atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg
     3601 ctcttgaatg acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag
     3661 ctgcatcttt ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc
     3721 aatcatcagt tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata
     3781 catcttactc atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc
     3841 gggtgtttgc atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg
     3901 cagcacggtt ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag
     3961 aacaaggaag aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg
     4021 taaaaaaccg cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt
     4081 gactggtaat gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct
     4141 acctaggttc ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt
     4201 caatgtttca ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg
     4261 ttcgttcgac tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt
     4321 ttcgacatct aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg
     4381 ttccacgatc tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact
     4441 aactaaaaga ataagaaaga agagacgttc ataaattttt ttccatttca tcgttttggt
     4501 tcctgaaact tatagccaaa tcctctaata gtcaccaagt actttggatg ttttggatct
     4561 cgctcgattt tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg
     4621 aagtcaaaat tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt
     4681 ttcatgaaat aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct
     4741 acggtcactt gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc
     4801 tcagcttcat cttttgattg cttacgaggt tcaatccgtc tgaatattgc tttcatacgg
     4861 gctaatactt cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg
     4921 ccaatgatcc gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt
     4981 ttttcttgac gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct
     5041 aaaatgatga aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg
     5101 gcactggtta ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa
     5161 ggttcatcat caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc
     5221 tgtttatatt ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta
     5281 taataggaag gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca
     5341 tgtttaggcg gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg
     5401 gaactagttg aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca
     5461 attcctagag atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa
     5521 gcaaaagtat ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc
     5581 aaagcctatc aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc
     5641 ccttcatgcg gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt
     5701 gccggagtgg ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa
     5761 tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct
     5821 ctttgaattt tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta
     5881 tgcagatgat ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt
     5941 atatgacaat atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct
     6001 tttttctaat tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga
     6061 taagtacccg gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga
     6121 ataaataaaa ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag
     6181 gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg
     6241 cgggaaatcc gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt
     6301 actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca
     6361 caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca
     6421 gagttaaata aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca
     6481 ggtaaaacga cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt
     6541 ccgctcctaa tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta
     6601 ggtcaacagc tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc
     6661 gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg
     6721 gcaggacgtc tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg
     6781 acgcaaccaa atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac
     6841 gtagctgata gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat
     6901 ggggatactc gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa
     6961 ttcatcggtt ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct
     7021 agccgtatcc ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac
     7081 gacgagaaaa aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat
     7141 gactttattg aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa
     7201 atgatcccag gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat
     7261 gttgaacgca aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat
     7321 ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa
     7381 gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa
     7441 ggggatatga acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta
     7501 ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa
     7561 taagatgaaa aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat
     7621 cagcaatttt tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga
     7681 tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt
     7741 cctgaatatg atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga
     7801 ttgagctggc agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc
     7861 gatatcaaaa aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa
     7921 ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg
     7981 aaaattcaag aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa
     8041 atcgttgatt cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat
     8101 gaattagcaa aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat
     8161 gcttttttag aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa
     8221 gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt
     8281 gctataagtt tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac
     8341 actccaacag gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc
     8401 atgggcagaa aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca
     8461 gagaaaaaga atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc
     8521 gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc
     8581 tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa
     8641 tggtatatcc agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga
     8701 acatatgaag cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg
     8761 cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc
     8821 tgtacatagt tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa
     8881 atctagatac gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc
     8941 attttcatca attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg
     9001 cacattcaaa aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc
     9061 acctggtgat gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg
     9121 tcttgagcct acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg
     9181 agtaacgaaa acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc
     9241 aggagcctgt tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac
     9301 aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt
     9361 gtaagaatct tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt
     9421 cctccttcaa actatctata ataatcttac aaaagataag taactggagc aaataaaaac
     9481 acttactgac aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta
     9541 aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta
     9601 cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa
     9661 ataatggcag ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt
     9721 atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact
     9781 tacaatcctt tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg
     9841 ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt
     9901 atgcaaaaac accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt
     9961 taagcgactt agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt
    10021 tagacgtcgt agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata
    10081 ttggccgtat cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg
    10141 gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt
    10201 aaataaacgt gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga
    10261 agaaatgatt atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa
    10321 cagccaatga gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt
    10381 tcggttactc aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc
    10441 agtccagaaa aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca
    10501 aataatgaga aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg
    10561 cctctagatg taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa
    10621 ggttattcta ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg
    10681 aatcaagaaa cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa
    10741 acaatccaag ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt
    10801 tctcgaatca cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa
    10861 ggaaactggc gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta
    10921 gctaaagaaa tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt
    10981 aaaattactg ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa
    11041 ccgaaaaatg cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt
    11101 tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca
    11161 caggaagtaa tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa
    11221 agaaaactag aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg
    11281 atataagttg actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg
    11341 cgaagtccgt gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt
    11401 gctaacacta ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa
    11461 acataaaaat tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa
    11521 atatcgggat ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga
    11581 attctattac catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa
    11641 agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa
    11701 agctgatata ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg
    11761 agtggtccgt gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt
    11821 tatttccaag aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata
    11881 aaggattatt agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga
    11941 cagtagatga ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct
    12001 atgataacat ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc
    12061 ttgacccagc tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac
    12121 acttagtatt catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga
    12181 cagatgaagt ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca
    12241 tgatcgatgc aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa
    12301 cggattctta ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata
    12361 aagggatgaa agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc
    12421 aattaaaaga atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg
    12481 aatggacacc tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa
    12541 cagcagatga agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt
    12601 tagtatttta tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag
    12661 ctcataagaa tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa
    12721 caaaagaaca attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc
    12781 acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca
    12841 aacgccgcgg tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg
    12901 gcgttgaacg tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct
    12961 acggtaaagt acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc
    13021 gtatcaaaga aatcagaaga taaacacgga aaacttcctt ggaaatctga aatttgtgaa
    13081 aagtccttgt atatcaaggg ctttttcttt tttctctatc acagttttaa gccatttttg
    13141 gctgttttgg accatttttc aatcatattc ccttaaaaat tgtcctagat tgtccgacat
    13201 tgactatcag tgtcgtcaaa atagggcaaa atttagtagt cagcttgacg acaaatataa
    13261 tacgaaaaat accaaaacat ttaccgaatt tagcaacttt tgaacccaag tttctcaaag
    13321 ggtcagctat tagcaaaaaa gacaaaacat gtacttttct actaaaaagt tgatgtgata
    13381 ggcttctatc taaataacta ttaggagttt ttatcgtgaa gcaaaatgat ggacgtatca
    13441 tttggaaaaa tcaaagtgag ttaaaactga ttttaacaat taatgaattc atcgaaaaac
    13501 acggaattac atctagccgt caataccaga aaaagctatc agaaaatcca aattctgcac
    13561 caagtatgtg gttcattaac aaaaaatatg gatcatggga gaatttattg attagtatag
    13621 gacgagaaaa tactgggtat ggaaaatggg ctagaatgtc agaacaagaa ttattggaaa
    13681 tagtagaagc ctttattaag tgcgagaaga taacctctca acgaatgtac gaacaaaaat
    13741 cagtagggaa gaatattcct tcacttagta ccattaaaaa gatgttagga gatatacgtc
    13801 cactttttaa aaaaaaaaat gatggatctc gatttactga tttcgaactg ctgttagaac
    13861 taaaaaatga aataatccga ttaaaactac aggatgattt atcaatgaca aagtttcgaa
    13921 aactagttca atccccaaaa ctaccatcag ttgatacaat tatgaaaaga acgaacaaaa
    13981 attgggagga attgatggca gagattggat ttgattatcg aagaatcaaa atttataagc
    14041 agagaaacaa cctatccaag acaaagaaaa ctaaatagta tcactctaaa tcagacgttc
    14101 aaaattaaaa tttggacgtc ttttttatta cccaaaaacg aaaggagatt tattttatga
    14161 aaaagccaag atttaagaat tggcgtttgc tcgcaacact gagtctatta tgccaaacaa
    14221 ttggtggatc gcttggccca acgattgcct ttgctgatga aattactcat ccacaaacgg
    14281 taactgtgga attggatttg gctcaccagt atgctgtcga aggtacattt agcgatggtc
    14341 gtcccatgtc agaagttacc gttccgcact atgcagttta caatggtgtg aagcaagata
    14401 ttttctgtat tgagccaggg gtgccgattt acaatgagtt cactcctggt tatgagaaaa
    14461 atccattgcc tgatatgtcg gagaaagcga aattagtttc cgttttatgg aaggaagctg
    14521 gtacggatgt ggatacacat attgtggccc agaagatgat ttggcaagaa gttaatggct
    14581 acacgcttca ctcaattaaa cgttctgatg gtagtgcagt aaatatcgca gccattgagt
    14641 caaaaatcaa tcaagccatt gcggattatc aaaagaaacc aagcttccat aactccacag
    14701 tgaaaaccgt gttaggtcaa tcgactactg tgacagatac gaacggcttg aatttatcgg
    14761 agtttgatga agttgtcgag aatatcgcaa atattgatta tcgtgtgaac ggcaatcaac
    14821 tagtgattac tccaaatgcc aattcaaaag agagtggtga attaacccta aagaagtcag
    14881 ctaatacagg aacaccggta gcttataaaa tggctggtca acaaactttg atggcggggg
    14941 caattgataa gcccaatacc tacacaatca aaattgatgt ggaaactgag ggttctttga
    15001 agattaaaaa agtcgataaa gaaacaggaa atgttgtacc gggaaccgtt ttccatttag
    15061 actttggaaa aacattacct gcaaaagacg tgaccaccga taaagaaggc attgcgacat
    15121 tggatgagat tcctcatggc gcgaaagtaa ccattactga aaaatcagtg ccagctcctt
    15181 atacgattga cactacacca atcaccgcaa ccattaaatc tggtgagact atttctgtga
    15241 cttcaaaaaa tactcgcgaa aaaggtcaaa tcattttgaa taaaatgggg gtggaaacag
    15301 gaactgatct ttggaacgac aaatactcat tagccggaaa tacgtttgcc attcgcaaag
    15361 acagtccaac cggtgaaatt gtccaagaaa tgaccacgga tgaaaaaggt catgcggaaa
    15421 caccaaaaga gattgccaat gcgttggaat tgggaaccta ttatgtgacc gaaaccaaag
    15481 ctagtaatgg ctttgtgaat acctttaaac cagtaaaagt cgaattgaac tatgtcaatc
    15541 aaaccgtggc tcttgttacc agtaacgtaa aagggcaaaa ccaagaaatc actggtgaaa
    15601 ccactttgac aaaagaagac aaagataccg gtaatgaaac tcaaggtaaa gctgagttta
    15661 aaggagctga atatactctc tttactgcaa aagatggtca agcggtcaaa tggagtgagg
    15721 cttttaaacc agagttagtg aagggaacga aagcttctga tgaaacggtg actttgactt
    15781 tagatgaaaa gaatcaagtt gctgttaaac acttagcgat taatgagtat tactggcaag
    15841 aaaccaaagc acctgaagga tataccttgg atgaaacaaa gtaccctgtt tccatcaaaa
    15901 aggtggatga taacgaaaaa aatgccgtga ttacccgaga tgttacggca aaagaacaag
    15961 tcattcgctt tggctttgat ttctttaaat ttgctggatc agctgctggg actgccgaaa
    16021 ctggcttcaa tgatctgacc tttaaagtat cgccattgga aggaaccagt gagattacag
    16081 atgccgaaga tgaagcaatt acagcttata acgagcaact aggttttgat gg
//
LOCUS       DACEPW010000049        16025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEPW010000049 DACEPW010000000
VERSION     DACEPW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.050"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(73..693)
                     /locus_tag="IW819_001248"
     CDS             complement(73..693)
                     /locus_tag="IW819_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1244363.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(769..1206)
                     /locus_tag="IW819_001249"
     CDS             complement(769..1206)
                     /locus_tag="IW819_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1244364.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1369..2199
                     /locus_tag="IW819_001250"
     CDS             1369..2199
                     /locus_tag="IW819_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1244365.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2183..3550
                     /locus_tag="IW819_001251"
     CDS             2183..3550
                     /locus_tag="IW819_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1244366.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3569..4378
                     /gene="yidA"
                     /locus_tag="IW819_001252"
     CDS             3569..4378
                     /gene="yidA"
                     /locus_tag="IW819_001252"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1244367.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4421..4864)
                     /locus_tag="IW819_001253"
     CDS             complement(4421..4864)
                     /locus_tag="IW819_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1244368.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4998..5369
                     /locus_tag="IW819_001254"
     CDS             4998..5369
                     /locus_tag="IW819_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1244369.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5538..6320)
                     /locus_tag="IW819_001255"
     CDS             complement(5538..6320)
                     /locus_tag="IW819_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1244370.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6317..7288)
                     /locus_tag="IW819_001256"
     CDS             complement(6317..7288)
                     /locus_tag="IW819_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1244371.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            7380..8774
                     /locus_tag="IW819_001257"
     CDS             7380..8774
                     /locus_tag="IW819_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1244372.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            complement(8966..10321)
                     /locus_tag="IW819_001258"
     CDS             complement(8966..10321)
                     /locus_tag="IW819_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1244373.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(10351..10986)
                     /locus_tag="IW819_001259"
     CDS             complement(10351..10986)
                     /locus_tag="IW819_001259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1244374.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            complement(10993..12369)
                     /locus_tag="IW819_001260"
     CDS             complement(10993..12369)
                     /locus_tag="IW819_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1244375.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            complement(12362..14143)
                     /locus_tag="IW819_001261"
     CDS             complement(12362..14143)
                     /locus_tag="IW819_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1244376.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            complement(14175..14486)
                     /locus_tag="IW819_001262"
     CDS             complement(14175..14486)
                     /locus_tag="IW819_001262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1244377.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            complement(14476..15462)
                     /locus_tag="IW819_001263"
     CDS             complement(14476..15462)
                     /locus_tag="IW819_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1244378.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            complement(15477..>16025)
                     /locus_tag="IW819_001264"
     CDS             complement(15477..>16025)
                     /locus_tag="IW819_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1244379.1"
                     /translation="EAAEQERAALEQEERMKIDQNFEQKRTQVETEHQKQKEKQIELL
                     EKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQIQFIKNALYSLP
                     LTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGFLINDQGVQYNFL
                     FSSLVQDIQGTMSFEIANQLFE"
BASE COUNT         4725 a         3474 c         2758 g         5068 t
ORIGIN      
        1 aaaatcgtgt cacacctcct tattctgata ggctgatcgt ctctgtcaca acctctcgta
       61 agaaaataat gtttattctt tttcagagtc agaaaattct tcctcttcat aatcttcatc
      121 atcatcgtct tcgtctatga tacttaaatc ttcttcgata tttcctggca agtcttcttc
      181 atcatctgtc gggtcatctg ctccgatttc ttgcaagtca gaattataag cagcgatttc
      241 ttcatcttca tcatcatcgt catcaaacag atcatcgtca tcttcttctg tcagatctgt
      301 atcttctggg tcatcgtcat tgtaatcgat cgcgtcttca tcatcattga taaaggcgtt
      361 gactttcttg cgtttacggc gacgtggagt atcttcatcc tcttcgtcca ttccatggtt
      421 tacttcttca tcgatcgaat cgatagcata ccatgaacgc aagccccaac gattatcccc
      481 taatgagata aagctgccgt caatattcaa gtcagtataa aattgtgcga gtgaatcacg
      541 aatttcactg tctgatttcc caagatagtt ttggatctgg ttagctaaat cagaaaaatc
      601 catgacatcg ccacgttgtt ctaagatcgc gtgtgctact tcgatcattg ataattcgct
      661 tttgtttgca ccttcaaata cactaatttc caatcaggac acgtcctttc acagtctact
      721 ccttatcata caaaatcaga cacaaaaaat caaacttttc ttttatattt acgctgtaaa
      781 atccaaggtc aaataatatt cgccgatttt ttccggtccc atgaacagat cataatcgat
      841 tcgaaccttc cctgctgtgg gacggtcttt caaactaaca tgcagttctt tcgtaaaagt
      901 agcaatctgg atcataccgt atggtgttcg ataggatgtt tctaaccgtt ctttatagcc
      961 aaatttcaaa cgcatccgca tttctcctgc acggatcagc tgtacatgac catctggaac
     1021 taatttgatc gttacaggaa tctcatcgcc cgtctcctcc tgaatctctt tataacgtat
     1081 gtacagcgta tcgccaattg tcaccatttg ccctttcaaa tcaaaataaa aatcttgcac
     1141 ttccccttgc tgttttactt ttgtacgcaa tttgattgaa atcggtgttc cttgtgataa
     1201 ttccattatt cattctcctt tacttctcat agctacattg ttcctagttt cttttgtttt
     1261 gtcaaccaac ttgtccattt ttacagaaaa ccttttttcc aaaacaactc tttgatttta
     1321 gagtataata ttgtagaaaa tatgaattta tacaaaggag ccggttgaat gaaccctgcg
     1381 attcgactag atcagcacat ttatcaaaaa aacgactttt ccccttttgc attaacagat
     1441 atattaacgg aatattctaa agaacaccag caaatctttt tacatttctg gcaatatgac
     1501 tgcacagtca tcttaggtat gaaggatgtt cgaactcctt ttctaagtga cgggatccaa
     1561 acactggaag atgcaggtta cgctccagtg atcagaaatt caggtggatt agggatcgta
     1621 tcagatgaag gaattttgaa tatttctttg attttccccc aaagtgacac aaaaaaaata
     1681 accatagatg acgcttacga gcaaatgctc ctattgacta aatacgcttt tccaaaggca
     1741 ccgatacaag cattcgaaat cacccattct tattgtcctg gaacttatga tttaagcatc
     1801 catggcaaaa aatttgccgg tatcgctcag cgtcggatca aaaatgggat ttctgttatg
     1861 atgtacttaa gtgtcaacgg taatcaggaa aaacgtgggc aagtcatgaa gcgattttat
     1921 caatctgctt taaaagaaga ctttggtaca aatggctatc ctgcagtgga tcctgctaca
     1981 atggcaacac ttgaagaact gcttgaccaa cccctttttg taaaagaagt caaagaagca
     2041 tttgcaaaag cttttgaaaa gctgtattct gcaagtgatt tacaggattc taccgactgg
     2101 ctaaaaaaag aaatccagct ggagcaatgg aacaaccaaa tccagagaat ggaacaacgt
     2161 aataacataa aggagctaca ttatgacaac cccttataaa tatcaaatgc tgcctatgga
     2221 aaaagttttt cgtgatccag tccataacta tatccatgtc cagcatcaag taatcttaga
     2281 tttgatcaat tcgaaagaag tccaacgtct acgacgcatt aaacaattag gcacttcttc
     2341 tttcactttt cacggagcag aacatagccg ttttactcat tcattaggcg tttatgagat
     2401 ttcaagacga atctgcgata tcttttctcg aaatttttca aaagaaaaaa taggcaacgg
     2461 aggctgggat gatcaggaac ggctggtgac tttatgtgcg gcactgttgc atgatgtcgg
     2521 tcatggtcct tactctcata catttgaaca tatcttccat acgaatcatg aagccatcac
     2581 tgtagagatc atcacttctc cagaaacaga agtgtatcag attcttaacc gagtagaaga
     2641 aggttttcct gaaaaggtag ctagtgtgat ccaaaaaact taccccaacc cacaagttgt
     2701 ccaaatgatt tctagtcaga tcgatgcaga tcgaatggac tatttgcttc gggatgctta
     2761 tttcactgga acagaatatg gaacatttga tcttacccga atattacgtg tgattcgtcc
     2821 ttatgaaggc gggatcgctt tttccatgag tggcatgcat gcagtggaag attatatcgt
     2881 tagccgatat caaatgtatg tgcaagtgta cttccatcct gtttcccgtg gtatggaagt
     2941 aattttggag catctgcttc atcgcgcaca cgagttgtat caagaagatg ccagcagttt
     3001 tgaactccat tctcaattac ttctgccatt tcttgacgga agctttactt tagaagaata
     3061 cctcaaactt gatgatgggg tcttgggcac ttactttact caatggtctg aatctgctga
     3121 tcctatttta aatgatttag ctaaacgttt tttgaatcgt aaaccactga aatctgctac
     3181 tttttctggc aaccgggatt caaaattagt ccaagaactc actttgttag tagaaaaggt
     3241 cggatataat cctgtttatt acactgctgt gaattctagc tacgatctac cttatgattt
     3301 ttatcgccca gaacaaggca gacatcgcac acaaatcgag attttgcgaa atgacggaac
     3361 tttgatagaa ttatcacagg ttagtcagct tgttgctgca ttagcgggac aagaacaagg
     3421 agatgagcga tttttctttc caaaagaaat ggttgatccg tccttgcgtg atcattatga
     3481 tttatttgac gaaacttatc aagaatttgc cagccatatc cgtaatggcg cattgattga
     3541 aataaattaa aatatattgg aggaaattat gtctatcaaa ctagtcgcta tcgacatcga
     3601 tggtacttta ttaaatgaga aaagagaagt aacaaaagaa gtaaaagaag cgattgccgc
     3661 tgctgttgca aaaggtgttt cgatcgtttt atgcactggc cgtcccttac caggtgtcca
     3721 ggaacagtta aacgagttga acctttttca agataatgat tacgttatta cttataacgg
     3781 ggcactcgta caacaaacga aatcaggtaa aatcatcgcc cgtcacggac tgacacatga
     3841 agatttccta gagatcgaag taatggcacg ccgtgtcggc agtcatctcc attcgatcga
     3901 tgacaaagcg atctacacgc ctaatcgaga tatcagcgct tatacgatcc acgaagcaag
     3961 tttagtcaag atgccgctaa aatatcgaac acctgaagaa atgacaccag acatgaacat
     4021 cgtcaaaatg atgatgatcg acgaaccaga aattttagat gcagcaattg cgcgtttacc
     4081 gcaaactttc cgtgataaat acacaacggt caaaagtgcg ccctattact tcgaagtact
     4141 aaataaagaa gcaagtaaag gtgcggcagt tgcgaatctc gcacagcacc ttggtattga
     4201 tcaagatgaa atcatggcta tcggagataa cgagaacgac ttatccatga ttgaatatgc
     4261 tggtctagga gtagcaatgg gaaatgccgt ttctcttgtc aaagaagcag caaatgtcat
     4321 cacttcatca aacgacgagc acggtgtagc tgaagcaatc aaaaaatatg tactgtaata
     4381 aataaaaaaa ctgagagaaa gctgatctac tcgctttctt tcagttttta tttttttcga
     4441 tccatctctc gactgcccat cggtggctac cacgtttcaa tttcacaagc gcttcttgag
     4501 ggctcaccca ccaaatttta ttggtctttt cagttggttc tcctatacgc ttccaagaat
     4561 cagcaacaaa gaaatatcct ggattataat aataagttgc acgataattc gagtaaaaat
     4621 actcatcagc ttctcccaga taagaagaaa tttctgccac aattcccatt tcttctatca
     4681 gctcacggac aatcgcttct tctttcgtct cttctccttc aatttccccg ccaggaagaa
     4741 aaaaagcacc atttggagcc tcgattactg caatctgctg atctctttct atgacaatat
     4801 atgctgcata acgggtttga taatcagcaa attgcttttt ttcaccaaaa attggtttat
     4861 ccatgaatag tttccacctt aacagtttta ttttctatcc attttacatg agaaatttct
     4921 catgaacaag cacaaatcaa ctatttcctt acttcttttt gttatgattg agtaaaagga
     4981 aaaggagaga tgaatctatg aaaatggctc acacatgtgt tcgagtgaaa gatcttgaag
     5041 catcaatcga attttatcaa aaggcatttg gttttgaaga aagccgccgc cgtgattttc
     5101 cagaaaacaa atttacactt gtttatttga cacttcctgg ggatgactat gaattagagt
     5161 tgacttacaa ctatgatcat ggtgcgtatg atctaggaga cggctatggt catatcgcta
     5221 tagcagctga tgatctagaa aaattacacg agcaacataa agctgctggt cttgagatta
     5281 ctgacttaaa aggattacct ggcactgcgc cttcttacta ctttgtcatt gaccctgatg
     5341 gttataaaat cgaagtcatc cgaggatagt tagtatgggg cattttactc ttgattgaat
     5401 aaaaaacaaa caccaataat taaaatgaga cgcaaacttt tcattaaatt gaaaaagttt
     5461 gcgtcttttt ttaacaacca ttgtagctat aaggttgcca ctctccacca atttattttt
     5521 aattcatttg aaagaaatca aataaacgag gtacctttag aaaacaaaac tgcttttcct
     5581 cctattatca cacgatcttc ttttaattca cattctagat ccccgcccct tgatgacatt
     5641 tgataagctg acaattttgt tttattcatt ctttctgccc aataaggaat aagattagaa
     5701 tgtgctgatc cacatactgg atcttcattg attgttaatt ttggaaagaa ggttctagaa
     5761 acaaagtctt tttgcacact ttctgctgtc acgatgactc ctattcctaa ttcaaaattt
     5821 ttgatagctg taaaatctgg ctgcaattta gccaccgttt tttcatcttc taatacgaaa
     5881 aataaatcac gtgctaagta ggcttcttta atcttagccc caatcgcttc ttcatattca
     5941 gacaatatcg gcactttttt cggcataata ctaggaaaat ccatgtaata ataatcttta
     6001 attcttgtca caaatagatt accgctttga gtagaaaatt taatcgtttc atcaggtatt
     6061 ttgtaatagt taaataaaac aaatgctgtt gctaatgtcg cgtgaccaca aagatcgatt
     6121 tctcgatctg gcgtaaacca tctaagctca aattcttggt ttttcttcac tgtaaatgct
     6181 gtttcagata aattgttttc aatggcaatt ttttgcatcg taccttctgg cagccattct
     6241 tctagcacat aaaccgctgc tggatttcct ttgaacacct catctgcaaa tgcatcaact
     6301 acgtaataat ctaagttcat tttaaagccc ctttctctaa tttgttataa atatattctg
     6361 cacaacaaag atcttcaata gctagaccaa ctgcgtcgaa aatcgtcggc ctcgattctc
     6421 ctttataaaa tgtttttttc tcagaaacta attcgcctaa tgtaccaaca atacaagatt
     6481 cctctaaatc tccttctgcc atggctataa gcaggtcccc gctttctgtt aaggcagctg
     6541 agtaatcatc tacatagatt tgagaagttt ccatcaacga tgtacttatc tctcttgtat
     6601 caggagtaaa tgtgcccaca gcattgatat gtgcgtcttc ccttacccac tctttttgaa
     6661 gaaatgcctc tttactagag gtcaacgtac aaatgatttc cgctggacgt actgcatctt
     6721 ctactgatat tgcatttata aacgttattt taggatactg ttttttagct ttagcgataa
     6781 aagtatttcg tctttcttct aaaatatcgt aaacaaacac tgtagtgatg ggacgaatgg
     6841 tagaaatcgc agtcaaatgc gacaatgctt gttgaccagc tccaattaaa gcaagtgttt
     6901 ctgcatgcgg atttgctaag taatctgttg ccagcgcaga tacagcagct gttcgaatcc
     6961 aggtgactgc atttgcgttt atcaatgctt tgggtagtcc gttttgagca tcaaacaaga
     7021 aaatctctcc taaatgagaa ggtaaatgat actgatgatt atccggaaaa gcggtaatga
     7081 cttttgtgcc aaaatatctg ttctccccca aataagcagg catcatcgca aacatatttt
     7141 tgccttttcc gtcaggtaaa atttccacca atcgaggatt ttggctgatt ttaccacttt
     7201 cataatcaaa aaaacatttt ttcattactt gaatcgcttc ttcaaaacta aaagaagcta
     7261 ctacttcctc aaaattaata attttcatag acaattccct cctcacaaaa gattaacttc
     7321 taaaatcaat acaatcaatg gtaaaattgt atggtataca attttaagga gtggagaaaa
     7381 tgcctattaa ttcttatgac aattacccgt taacttggcg accagacaaa caaaaattga
     7441 agcgtcctta ttatcgatcg ttgattcaac aattagaaca agatatttta aagaataaat
     7501 tacagaaaaa cactcagtta ccctcccaaa gagagctagc tgattattta gatttaaatt
     7561 ttactaccgt tggcaaggct tataaatatg gaattgagaa aggtttgctg tatacgaata
     7621 ttggaagtgg cacatttatt tctcccaatg tctttgagtc catcaccatc tctaccaatg
     7681 aagtaaccga acatatcatt gattttggtc tggttagttc ttttgaagaa tgtaataaat
     7741 tcgttgtccc gtttattcaa tcaactagta ataacccaaa tcttgttggt ttgcttaatt
     7801 accaagatcc caaaggcaca aattttcaat tgactactgc agcaaattgg ttaaaaactc
     7861 aaggagtcat tccgtcctta gaatctctat ctattgtttc cggtgttcaa aatggattgg
     7921 ctatcacact ttctgcactt ttctctccag gtgatagaat agcaacagac cgatatacat
     7981 acgctaattt cattgaattg gctcagttat atcatttaga gatcgttcca attgattttg
     8041 acgagcaagg aatgcgacct gatatattga ttcaggaatg tagaaaaaag aagattcatg
     8101 gtgtctttct tatgccttct tgtaataatc ctatagggtt tcaaatctct cacaaaagac
     8161 gagagcaatt agccgaagtg attgaaaaag aaggtctatg ggttattgaa gatgatatcc
     8221 attcattttt aacaacttac tatcaacata cgatcattcc tcctttccaa aatttattac
     8281 ctaaccaaac aatctactta gctggaatga caaaatttat ttgttctgga ttgcgagttg
     8341 cttatcttgt agttccagta catgtccaaa gcaagataga acaagcaatt tttaatataa
     8401 atgtgaaaac ttctggatta gatgtggaag tcatcactca aatcctccat tcaaatgaag
     8461 caaataggat cttagaaact aaattaagtc ttacaaaaag agccaatcag ctttttgatt
     8521 ctcttttcaa agtagaaaaa ccaacgaatc gtgtccctta ctatcgaatt cttccagttg
     8581 atcctgagat tgaccctaag ataattgaga atgactttct tgctaacggt atccgacttt
     8641 atcattccag acgctttact acacgtgcgc aaccagatgc attcgttcga atttccctat
     8701 cctctaataa ccttgaaatt ttagcttctg gactaagtat cgtaaaagaa aaaataaaaa
     8761 aatatgaatt ataacctatt gataaataaa ggaatcaaat cttattgatt cctttcaata
     8821 aaaaagaggc actacccgag gttcaacaac gggtagtgcc tcaaaaaata aacgaacatt
     8881 ttgcacttaa agtatccaag aagcaagaaa aattatttta ttttcttatt ttactaaatc
     8941 actaggtttc tgtcagaaga tgaaattatc caatcatgac actttcttct ggatatttgt
     9001 attttgattg tgttctttgt gcttttgtca tcagagagaa gaccacagtc aatacaccaa
     9061 ctcgtccaat gtacataagc gctataatga tcaatttccc tattgcagtc aagtctggcg
     9121 tcaatcccat cgtaagacct actgtaccaa acgcagagaa gacctcaaaa gcaatatatt
     9181 cgatgccaga agttttaggt atcgtttcag tcaccgataa aatcatgatg gcgactacac
     9241 acaacgtcaa cgtaacaaaa aataaagtaa gtgcccgcaa cactgcgcct tgtcggatgg
     9301 tacggccaaa tgcttccgct ctcgtacgtc ctttcaacat cgaacgcatc tggatcagca
     9361 acacacctaa cgtagttgtt ttcaatccgc cggccgtcga cccagatgtt ccgccgatgt
     9421 acatcaaaaa catcgttaaa atcagacctg catggctcat atggaggtaa tcaactgaat
     9481 aatatccagc cgttctaggt gtcacactca taaaaaaggt gtttgccaat cgttgaaaca
     9541 tattcccttc cactagcgtt gccccattgc gctctgtgaa aaagaacaca acgaatccgc
     9601 ctaataaaag ggtacctgta acggttaatg cgatttttga atgaatcgtc atcttctttt
     9661 tctgatgata ttccagtaaa tcacgccata cgatgaagcc taacccacca gaaatgatca
     9721 aagcagatac caccatgatc aaataaacat tttgctggtg attggctaaa ctgtctccta
     9781 acaaatcaaa ccctgcatta caaaaactag agattgcatg gaacaaactg taccagatac
     9841 ctttcaacca gccaaattca ggcacaaata caaaagataa agcaaccgta ccaatcaatt
     9901 ggatcacaaa ggccaatttt aggatgtaaa gcatgagctt catcacacct gatacttctt
     9961 ctagattcag cgcttctttt aatataatac gtgtgctgag actgactttc ttctttatca
    10021 atgcaaagca tagaatcgga accatcataa aaccaagacc gccgatctca atcaaaagca
    10081 tgatcaaaaa ttgaccagca ctgttccaat gctctgctgt attcaatgtt gtcaatcccg
    10141 ttacacagac agcagaagtt gcagtgaaaa gcgcatcgat aaaatttgtc ccatgcccac
    10201 tattactaaa aaatggtagg gtaagtaggc cgccgccaac taaaatcagg agaaaaaatc
    10261 ctaatgcgag aagctgaaca gccggcaaac gtttctttat ttgtttgctt tgtttaatca
    10321 tcgttttcct cttttaaaaa aagataccac ttagtcagtg gtacccatat tttttacttt
    10381 gatcaacctt gttacttctg cgcgctcgtt ttcttccagc ttcatacgaa tataatagat
    10441 cgtttcttct aattgaggga tcgtcatata ttctaatgca ttgactcgcc gacgtgtctt
    10501 ttcgatttca ctcgccatca gttgacaagt tttttcgatt tctgtcagtt ccaaaagttt
    10561 tggaagcagg gtagtaaacc gatcgattga ccgatccagt tcggcactgg aattcaaata
    10621 cccgtattcc aaaggtgtgt ctgtcagctg ttcatcatat tcaaaattca tcacaggtac
    10681 ttttacactc atgatatttt tgtctacgat agacaaatca acgttttccg ccggcaaaac
    10741 aaataattct tcgatgaaag gttccgtgat cgaagcattt gccaatacaa aatcagacat
    10801 tgcttctgtc atagactctt ccatttcttg acgcagctgg ttgttctttc gaatcaacag
    10861 gatgaattga cgcatcagtt catcttgctt atcttttaat aatttatgcc ctcttgtggc
    10921 agttgtcaac tgttttttca atcgagtcaa ttccatgcgg gtcggattca cattcaatcg
    10981 tgccatcaga actcacttcc cttctggcag atatttgtcc agcatgtcgt ctttgattcg
    11041 ttttaattcg gttcttggca acatcgatag cagttcccac cctagatcaa gtgtttcagt
    11101 aatcgaacgg ttcgtgtaga atccttgatt gacatattct ttttcaaacc gctcagcaaa
    11161 cttcgcatag atcttgtcta catcagataa agcagattca cctaaaacta cggctaattc
    11221 ttttgcctgt tttccttgtg cataggctgc aaaaagctga ttcatcgtag ctgcatggtc
    11281 ttctcttgtc tttccgggtc cagttccctt atcttttaac cgcgaaagag acgggagtac
    11341 atcaattggt ggctgaatcc cactcttgta aagctctcta gtcaagatga tctgtccttc
    11401 tgtaatataa ccagtcaagt cagggattgg atgagtttta tcgtcttctg gcattgttag
    11461 aatcggaatt tgagtaaccg atccttttag tccgcggatt cttcctgctc gttcgaataa
    11521 agtagctaag ttcgtgtaaa gatacccagg atatcctctt cttcctggaa cttctcgccg
    11581 tgctgcagaa atctctcgca atgcctctgc atagttggtc atgtctgtca tgataaccaa
    11641 aacatgcatt cccttttcat aagcaagata ttctgcagca gtcaaagcca tacgaggagt
    11701 agcgatacgt tcgattgcag ggtcgtttgc taaattcata aacatgacag aacggtcgat
    11761 tgcgcccgtt tgacgaaaat cttccatgaa aaattcagct tcttcaaaag tgattccaat
    11821 agcagcaaag actacggcga aatcatctga tgaatcgagt accgttgctt gtcgcgcgat
    11881 ctgagcagct aattctttat gaggaagacc agagccagaa aatactggta atttttgccc
    11941 acgaactaat gtattcaaat gatcgattgc agagatacct gtttggataa actcatctgg
    12001 ataatcccga gctactggat tgattacttc tccgttaata tccaaatgct tttcaggcag
    12061 gatctctggt ccgttatctt tcggtcgtcc caaaccatcg aatactcgac cgatcatgtc
    12121 ttccgataca cctagttcta aaggatggcc taaaaatcgt acagaagagt cacgtaaatt
    12181 gataccactt gttccttcga atatttggac cattgcttta tcttcttgga cttccaatac
    12241 ttgtccacga cgaatctcgc cattttgcat acggacttcg attaattctt catatttgac
    12301 accagcaact ttttcaaccg ccatcaatgg accgacgact tcaccgatcg tacgatattc
    12361 tttaatcatt gctcatgcct ccttctgaaa cgatcgtttg catcgtttgc ttgatttcac
    12421 caataatttg atctagtttt tctaattctg tttccgggat atatttgctt cgtccgatcc
    12481 tttcacgaat cgatacggta ccgtccatga tctcacttaa ataagcacct aacgacaacg
    12541 cacggcgtcc ttcttcacca aaggtcaaga tcatcttcaa cattttgaat tgtttctctc
    12601 ttgaagtgaa tgtatcgaca tcatcaaaag cattttgctg caaataatct tcacgaatgg
    12661 attttgccac ttctaatgtc agacggtcat tatctgaaag ggaatcaatc cctaccaaac
    12721 gcacgatttc attcagctgc tgttcttcct gcaaaatacg catgccctcg gtgaccatgg
    12781 atgcccagtc agcttgaagg atatgatcca tatacttccc tacttctgtt gaataaagag
    12841 aatagctttg tatccaatcg atcgaaggaa aatgtctttt ttgggctaaa ctggaatcaa
    12901 gaccccagaa tacttttacg acccgcaatg tattttgtgt gactggttcc gaaatatccc
    12961 ctccagacgg ggatacagca ctgattgcgg ttatacttcc ttcgcgacct tctgatccta
    13021 atgcaatgac tcttccagat cgttcatagt attccgcaag ccgtgaacca agataagctg
    13081 gataaccttc atcaccaggc atttcttcca aacgtccgct catctcacga agtgcttctg
    13141 cccatcgtga agtggagtct gccatgatag caacgtcata acccatgtca cggaaatatt
    13201 ccgcaattgt gattcccgta tagatagaag cttctcgagc tgctactggc atgtttgatg
    13261 tattagcaat caataccgta cgttccatca acgattctcc tgtattaggg tcaatcaatt
    13321 cagggaattc atttaaaaca tccgtcatct catttccacg ctcgccacat cccacataga
    13381 caaccatgtc cacatcggcc cattttgcaa tttgatgctg aacgactgtt ttccctgcac
    13441 caaaaggacc aggtacagct gctgctcctc cttttgtcac tgggaagaat gtatcaatca
    13501 ctcgctgtcc agtgatcatg ggtgcatccg ggtttaattt ttctttgatt ggccgactac
    13561 gtcttactgg ccatttttga agcatcgtca aatttttgtc accattttcg gtttcaatga
    13621 cacatactgt ttcatcaata gtgaatgaac ctttttcgat tgttttgatc gttcctttta
    13681 tcccatttgg caccatgatt ttatgtttta taacttttgt ttcttctacg atcccgataa
    13741 catctccagc actcaccgtt tctccggctt ctactgttgg ttcgaaccac cattttttct
    13801 catggtccaa tgcgggcaat tgaactccgc gccctaagaa attactttgt gtgatttcca
    13861 taaaggtatc taatggccgt tggattccgt caaacatttg tgaaatgatc cctggtccta
    13921 attcaacaga taaagcttca ccagtagagc gaaccggttc gcctggacca attccagaag
    13981 tctcttcata tacttgaata gacgccacat cgccacgcat ctcgataatt tctccgataa
    14041 ctcctagatc tcccactaga cacatgtctt ggatactggc atcagacatg ttctcagcca
    14101 ttacaagagg tccggagact tttatgattt ttccaatttg caaaatttct atctcctttt
    14161 catactattt catactataa gatattttga cctactgctt tttctacgtt tttttgaatt
    14221 tcttccagcc ctatccctaa tgatccttga tgattgggaa tcaatacgat agcaggtatc
    14281 atttgttcct tgtaacgttc gatcgtttct gggctcaatg ctgcacattg ctctgtgaca
    14341 taaatcacgc catactcatt ctttgccatt tcttcaattg cctgacgaac ggtttgcttg
    14401 gttgtaccat aacgaacatc aaagccaaac aatttgaatg gcaaaacaga atctttgtcg
    14461 ccaactacac cgatcttatg cgtcatagac ttgtctcacc cgctttctga ttgtttctga
    14521 ttcgaaatgg cttcttttgc cgataaccag taaacgaagg tttttgcttt ctacctcttt
    14581 tgcgttcaaa aaggctagta gtggcagtgg tccgaaagcc tgggtttgtg cttcttgata
    14641 caatgaactt agataatcat ctttcaactg ttcaagcttt acgagatcaa tatttccctc
    14701 acggatgaca gggcgcagaa aatcagcata atctgtctct aatagaaaat tcgtgtaatc
    14761 ttccatatct ttatctacaa agttcaaaaa tacttctttt tcgatacttc ccgagctgga
    14821 aagtaccgtc gtcatgaacc catatgttct tttctgcaat attcctcgag ccatcgttgt
    14881 gatattggtc aagtcaataa aagcaatgac ttctgtcagc aattcttgat atcctagctt
    14941 atcggcaagc cgtcgctgct ctgtcaaaaa ctgtcgatca taaatcacgt caattccctg
    15001 aagaatggta gactcttcaa aataatcacg tacttctcgt atactgtcca taatgctttc
    15061 aggaagttca tttgaaactt gcgtatgaat ggcatccttc aaggtttcta atgaataaaa
    15121 tccatcgtcg atatacaaat ggtccagatt ttgatttgtc atctcagctt ttgtcaaaac
    15181 cttcagatta tggaaagtat aacgcatcgt atagatccaa acgatttcag gctccggtgc
    15241 tgattccttt aaccagcgaa atagacggct ctgctccttt aaaagattct gttcgaagtc
    15301 atattcatat ccgtcataaa tatatggact gtaaatcgtg gatttcaata tttcaccaag
    15361 tgcttcgaca gaatttgttt gaatcatccg ttcaaatgtc tcattggaca aaagttcaag
    15421 ctctcttccg cgaatcaagg gatttagttc gtggtattcc atacactgat ccctccttat
    15481 tcgaataatt gattggcaat ctcaaaactc atcgttcctt ggatatcttg tacaaggctg
    15541 ctgaaaagaa aattatactg aactccttga tcgtttatca gaaaacccgc ttgatcagca
    15601 acagtttcat cacttaaaac catcataaag ggaagttcat tgttccattc atccaacaac
    15661 gtctgagaaa gataggctgc tgatttttct ccagcaatga atgctacttt tcctgtcaaa
    15721 ggtaatgaat acaatgcatt tttgataaac tggatttgtt cagattcatc ccaattttcc
    15781 atttcagtta ccgcatctgc aaacaatcgt cttaaaaatt cctgcttcgc attcaaattt
    15841 tcttgtcgta cttctacttg ctggcgattc cttagctgac ggtatttttt ttcgagtaat
    15901 tcgatttgtt tttctttttg tttttgatgt tctgtttcaa cttgcgtacg tttttgttca
    15961 aagttttgat cgatcttcat tcgctcttct tgttctaacg ccgctctttc ctgctctgct
    16021 gcttc
//
LOCUS       DACEPW010000050        15930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEPW010000050 DACEPW010000000
VERSION     DACEPW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.051"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(20..904)
                     /gene="rsmA"
                     /locus_tag="IW819_001265"
     CDS             complement(20..904)
                     /gene="rsmA"
                     /locus_tag="IW819_001265"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1244380.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="IW819_001266"
     CDS             complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="IW819_001266"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1244381.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1459..2235)
                     /locus_tag="IW819_001267"
     CDS             complement(1459..2235)
                     /locus_tag="IW819_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1244382.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2390..3712)
                     /locus_tag="IW819_001268"
     CDS             complement(2390..3712)
                     /locus_tag="IW819_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1244383.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            3887..4474
                     /locus_tag="IW819_001269"
     CDS             3887..4474
                     /locus_tag="IW819_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244384.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(4953..6962)
                     /gene="metG"
                     /locus_tag="IW819_001270"
     CDS             complement(4953..6962)
                     /gene="metG"
                     /locus_tag="IW819_001270"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1244385.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            7230..7508
                     /locus_tag="IW819_001271"
     CDS             7230..7508
                     /locus_tag="IW819_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244386.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            7605..8426
                     /locus_tag="IW819_001272"
     CDS             7605..8426
                     /locus_tag="IW819_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1244387.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8711..9721)
                     /locus_tag="IW819_001273"
     CDS             complement(8711..9721)
                     /locus_tag="IW819_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1244388.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(9733..10686)
                     /locus_tag="IW819_001274"
     CDS             complement(9733..10686)
                     /locus_tag="IW819_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1244389.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(10676..11746)
                     /locus_tag="IW819_001275"
     CDS             complement(10676..11746)
                     /locus_tag="IW819_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244390.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(11752..12795)
                     /locus_tag="IW819_001276"
     CDS             complement(11752..12795)
                     /locus_tag="IW819_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1244391.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(12796..13737)
                     /locus_tag="IW819_001277"
     CDS             complement(12796..13737)
                     /locus_tag="IW819_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1244392.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            13821..14168
                     /locus_tag="IW819_001278"
     CDS             13821..14168
                     /locus_tag="IW819_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1244393.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(14238..15899)
                     /locus_tag="IW819_001279"
     CDS             complement(14238..15899)
                     /locus_tag="IW819_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1244394.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         4482 a         3296 c         2863 g         5289 t
ORIGIN      
        1 tctgtcacaa cctctttttt tactgcatat tttctgacat cgcattgctt aatgcggcga
       61 attcttgtaa tgacaaagtc tccccacgac gttttggatc gattccagct gtttccaagc
      121 tttttgctaa ccatgctttt gtctggtcat ctttgccata cgaatgctgc aagttattcc
      181 aaagcgtttt tcttctttgc tgaaatgccg cttttgtcaa tcggaaaaat gctttttcgt
      241 ctgtcacttc gactgccggt atatctcttc ttgtcagttt caaaatcgct gaatctacat
      301 ttggctgagg gacaaatact gtttttggta cgatgaatgc taaactggct tccatataat
      361 attgaacagc aatcgataat gaaccataag ctttcgttcc tggttcagca gagatacgat
      421 cagctacttc tttttgcatc atgacgacta gttcatccac aaccaaatct gattcaagaa
      481 aatgcatcat gatcggtgtc gtaatatagt aaggtaaatt cgctacgact tttaacggaa
      541 gctcctcatg aaatgtttct ctaacagctg ttgacaaatc ggtctttaaa atatcttgat
      601 gtacaatagt cacattgtca tagggttgca tcgtatcagc caatacaggg atcaaacgat
      661 catcgatctc aaatgctacg acttgttttg catgcttggc taattgttcc gtcaatgcac
      721 cgattcctgg tccaacttcg atcacgttcg tatgttcatc tatttgcgca gtttctacga
      781 ttttccttaa aatatttggt tcagtcagaa aattttgtcc taaacttttt ttaaatgaaa
      841 aaccatgttt caataaaatc tcttttgttc tagagggtgt cgcaatttca cgatattcac
      901 tcatgttgtt cctccactat tttcattgct tgggcgagtt ctttcggcgt aatacgaaac
      961 atcttcaaac gtttttccaa ttgtttgccg ttcgtgtaac cgatgcgcaa ctcgtcacct
     1021 agtttttctc gtttttcttt tgcttgtgcc ccagctatta aaccatagtc taaaaggtct
     1081 tgtctagtga tttcagaaac agccgtttgg gaaatgtctg gcgtcacgac ctttttcaat
     1141 gcttctaaaa tagcagcatc acttgcatgc tcgacaccaa ggcttccctt tttctttggt
     1201 ttagcttgtg tgcgcggcaa aaaagcatgt tttgcctgag gaacagcttc cataatgatt
     1261 tttcttattt tttctcctga aaaatcagga tcggtaaaaa cgatcactcc acgagtctct
     1321 tgggcatgtt caatttgcat caggatctct tcattgattg cagaccctat tgtttctatc
     1381 gtatctgcat caactacttc ccgcagcctt cttgtatcgt cttttccttc aacgactata
     1441 atctcttgga tcttcatctt atccatgttc gcttagacca aataatcggt gcgcatttgt
     1501 ccatgtttgg gcagccactt cttctgtcgg aagattccga agttcagcaa tcttttctac
     1561 tgtataacga gtatagcctg gttcatttcg ctttcctcta aaaggaactg gtgccagata
     1621 aggggcatct gtttccacaa gtaatcgttc gattggtaca tttgctgcag cctcttggac
     1681 gtctaatgct tttttgaaag tgacgacacc gctaaatgaa atatgcatcc ctaagtcaag
     1741 aaatcgtttt gcccattcat aatcaccact gaaactatgc atgattccac cgatatctcg
     1801 aacatcttct tcttttagga tttgatacgt gtcttcaagt gcttctctag tatgaatact
     1861 gattggtagg ttcatttctt ttgcgatagc aatctgtctg cgaaatactt tatcttggac
     1921 ttctttcggg tcttccatcc aatgataatc caagccaatt tcccctaatg ctacaacctt
     1981 tggtaaagtc aattgttctt gtaatttatt ttcgatttct ttcgtataac tgccagcctc
     2041 tgtcggatgc cagccgataa tactgtatat atttggatag tcatggctta attgcaaaga
     2101 cttttcaatc gttggcgtat cgaagcccac gaccgccatt ttcgttaccc ccatttcttg
     2161 tgctcgctga atcgtttctg gaatatcttc attgaactgt tcagcattta aatgggtatg
     2221 agaatcaaat atcatgttta tctctccttt tcattcttac tttagcacat aagcactact
     2281 tgcagaagta gcaaaaatca aaatagaaga acttgtttta ttgaaataga aaaagcacga
     2341 agatctagag gacctagttc gtactttttc tgtctgttgt tgaaatattt tactcttctg
     2401 agcgtttttt ctttttttga taatagactg ggatacctat caaggtaaca cctataccga
     2461 tcaatgctaa tttcgtttgc gtcatcatcg tcatacctaa aatgaaaata gcaccgagaa
     2521 tcgcaataat cggaacgatt ggataaagtg gtactttata tggacgtggc aattccggcg
     2581 ctttttttct taaaataaag acaccgataa aaatcagtaa actgaatagc cacatcacaa
     2641 aaatcaacat gtctgttaaa aagtcgaaag aaccaaggct catcataata caagctactg
     2701 ctaactggaa aaccgcagga acataaggaa ccgtaaactt ctttgaaagc tttgtcagct
     2761 gtttactgaa aggtagctcg tcttctaaag ccatcgcata aggcacgcgg atcccagtta
     2821 gtgtataacc gttcaatgca ccgtataccg agatcaagat cccaatagtg accagctttc
     2881 caccgattcc tccaaaaatg acgtctgatg cttcagaagc tgcattcaaa ttaccggcaa
     2941 gatgatcaat cggcaaagtt ttcaaaaaaa caaaattgat caatagataa acgactgtta
     3001 caaaactcaa accaagaata attgctttag gcaaatcttt ttccggccgt ttcatttccc
     3061 ctgccattgc accgacacct aaccagccgt cataagcaaa caacgttgct aacaacgcac
     3121 tgctcaatcc ttcgacaaaa gtaacatctt ttccagcttc gattggaaat aactggacag
     3181 ttccttgtcc tggggtcaat aatccccata tgacaatcac agcaattggt attaatttaa
     3241 caatcaacgt cactgattgt acagaagcac cgacttttgt ccctaacaaa ttcaaccctg
     3301 tcacagaaac agcggtgatc actgcaatca acaaaagata atcggtagat aattgtaata
     3361 agttcgtcaa ttgtgtcgca aagataatcg ataatgcagc aatattagca ggaaagtaaa
     3421 tcaagctttg cgcccatcct aataaaaagc tgggcaattt tccatacgca gcttcaatat
     3481 attttacagc tccaccagtt tccgggattg ctgtagctaa ttcagccact gtcaatccag
     3541 cacagatcgt taagaaacca cccaaaagcc aagctagcaa tgtcaaactt gtagactgag
     3601 tactagcagt aacagcagca gttttgaaga agacgcctgc accgatcacg gttcccataa
     3661 cggtagctaa tgcgccaaac agattgattt ctcgttttag tttgcctttc attcccatcc
     3721 tctttcttct ttgaaatttg gtataatttt cagtataact gagaatattt taatttacca
     3781 ttcttttttc tccactttca gtgaaaaact ccctcttaag acggatgtat ttctttttta
     3841 tttttcgtat actaaaccta ccttttccac gaaggaggaa cagtttatgg attttaagcg
     3901 ttcttatccg aaattagcag attatttatc caaccgggtc gatttttccc gtaaagaaca
     3961 acagcagtcc cattggcatt ggttcacatt aattttattt ggattaatga ctgttgcaac
     4021 gttatcacaa aaactaatat tgctgacttt gttcatcgca atcgttgcat tgatcaaagg
     4081 tcctgtgatg atcttgtggg ggatgttgta ttcctttttg gttgggcttt ttccgcctat
     4141 cggtattttg ctttcagcga ttttcttttt attgaatatc ggaacattta caaagaactg
     4201 gcggatgacc ttagtcggga tctattttta tttctatcct tttggagtga tggcacttag
     4261 tgaagtcatg cattgggata atcattggtt tatagctggc tccttgcttt tagggttgat
     4321 cttgttacac gtgatgttga caaagctcta tcaacattat ggtatcggac gtacgatttt
     4381 ctggtatgtc ttttcgatcc catttgcact gcttactgct ctcttaccaa gccgtttgaa
     4441 gacaaaaata aaaggttacc ataaaatcaa gtgataggat ttttacaaaa taaagaaaac
     4501 aatcatcgaa gtcaaagccg atgattgttt ttttatctat attttcaatc acacatataa
     4561 gagcacaaac taattgaaca taaagtgata gaacttataa ttaacacatc aacgctactg
     4621 ttagatataa atatagatgt aataaagtca ttgcattcaa actttatgaa tgtaaggaca
     4681 ataaaaaaga gtaccagatg aatcaattaa aatctgcccg accctttatc attatgcttt
     4741 ttttctgttt ttacgattcc tattagtatt attctaaatt aacaaaaatc agcgaagctc
     4801 gtacttcttt ttctaggatt tattttggat agttctctgt ttctgtatta tatgattggc
     4861 ttcaagtaat aagaaggaat ttacgaaaat tgcttctcaa atttcggaaa aagggggtat
     4921 gacacaacta gtgtcatacc ccctttgaat ttctatgcga tttctgagcc atttggcatg
     4981 gattttggtg cttcgattac ttgtaaagag ccatctggtg cttctgcaga aaggatcatt
     5041 ccttgactga tttgtccacg catctttcgt ggtttcagat ttgctacaat gacgactttt
     5101 ttcccgatca attcgcttgg atcaggataa aattcagcga ttcctgacaa gatttgacgg
     5161 tcttggctgt ctccagcatc tagtcggaat tgcagcaact tatctgcccc ttctactttt
     5221 tgacagttga tgacttctgc tacttttagt tctacttttt caaatacatc aaatttgatt
     5281 tgtttttctt tcgttgaaac aagttctgtt tcttctggat cccatttgac tgtatcttcg
     5341 tttgtttgag taccttcaga cattttttct tgaataaaag caacttcttc ttccatatct
     5401 aaacgtggga agattggtgt accttttgca actacttttg ttcctgaagg aaattcgcca
     5461 aagtgtaatc cttcaagatt cattgtttca gagtccaatc ctagttgatt gaagatcttc
     5521 tctggcgttt ctgtcatgat tggctgaagt aatatcgctg cgatacgcaa agattcagct
     5581 aaatggatca tgacactatt caattcagct ttcttttctt cgtcttttgc taatacccat
     5641 ggttctgttt catcaatgta tttgtttgca cgagaaatca gcacccagat ttcagccaat
     5701 gctgtattga attccatttt ttccattgca tcatggtaac gtccgacaac gttagcagcc
     5761 gtcgtagaca gttcactatc aaatggagtg acaagtgagg catagtcagg aacgagtcca
     5821 tcacaatatt tgttgatcat agcaatcgtt cgattcaata agttccctag atcatttgcc
     5881 agatcataat tgacacggga aacaaaatct tctggcgtaa agacaccatc tgacccaaac
     5941 ggaacagcac gaagcaaata ataacggagt gcatctaaac cataacgttc cacaagcatt
     6001 tcaggataaa cgacatttcc ttttgattta gacatttttc cgtctttcat caatagccag
     6061 ccatgcccaa atactttttt aggtagaggc aaatcaaggg ccatcagcat gattggccaa
     6121 tagatcgtat gaaagcgaac gatttcttta ccgaccatct gaacatctgc aggccagtat
     6181 ttttcgaata gactttcatc ttcggagcca tatcctagtg cagtaatata attggataat
     6241 gcatcgatcc atacatacac cacatgtttc ggatcactct tgactggtac tccccaagta
     6301 aaagttgttc gtgaaactgc aagatcctct aatcctggtt tgataaagtt gttgatcatt
     6361 tcgtttttac gagattctgg ctggatgaat tcgggatgtt cttcatagta ttccaataga
     6421 cggtctgcgt atttgctcat gcggaagaag tatgattctt cttttaccaa ttctacttcg
     6481 tgtccacttg gcgctttacc accgatcacg ttaccttcgt catctttgta cacttctgca
     6541 agttgtgttt ctgtaaagaa ttcttcgtca gaaacagagt accagccttc atattcacct
     6601 aagtaaatat ctccctgttc taataaacga tcaaatattt tttgaacagc tgctttatgg
     6661 taatcatctg tcgtgcgaat gaatttatca taactgatat caagcgtttt ccataatttc
     6721 ttgacatcag ccgccatttt atctacatat tcttgcggtt ttacacctag ttcttctgct
     6781 tttgtttcga ttttttgacc atgttcatcc actccagtga gataaaacac atcaaagccc
     6841 atcaaccgtt tataacgagc aattgcgtca catgcaatcg tagtatacga gttgccgata
     6901 tgaagtttgc cgcttggata atagattggc gtagtgatat aaaaagtttc cttttctgcc
     6961 acggggctct tcctccttta tcgcatgatg cgaattcgtt tccttgctag tataccataa
     7021 aaaaagcatg ctgttagctt tttttcaaaa tgaaaaagac tggataaacg acttctatct
     7081 acgttcaatc tatcctttaa aaggatgaat aggttgaata aatgcgggtt tttgttttgt
     7141 ttcatatcta ctaaaaaacg gctttttatt tctcccctca cacgtggtat aataaagact
     7201 atctatctta gtaagggact ggaacaatca tgcagaagaa acatcttttt tttacactta
     7261 gtattgcttt tctttcatta gcacatttaa tattcagcta tttttatatt cgtatgtatg
     7321 gttattttaa cctccatggg catttgaaca gttttatgac agcagcttgg attttgcgtt
     7381 ttatcattga cgtatatatc gttatttgtg gcttctttgc cattcgtgaa gagcggtata
     7441 aagtattgcc gttttatctg cttttctttt tattcaatct catacttcca tttatttttc
     7501 atatttaaat gataacctag atttttcaag taaatccttg gttttttttc agagtctgct
     7561 acaataaggt aaaaagtact tttactgtca atggaggtgg tcagatgaac cgttttgctt
     7621 ctaaagaaga agaaaaatta tattatgaac ataaagcaag tgaagaagaa tttcttaact
     7681 ggtatcaaaa acaggaacgt ccagaatatg acaagccctc tttgactgtc gatatcgtac
     7741 tgatgtgcta taacaaagaa gaagatcagc tgaaagtttt gttgatccaa agaaaagggc
     7801 atccttatcg aaattcatgg gcattgcctg gtggctttgt ccaaaaggat gagtcaactg
     7861 gagaaagcgt cttaagagaa acaaaggaag aaactggtgt ggttatttca aaagaaaata
     7921 ttgagcaact gcatacattc agtacgccta atcgagatcc gcgaggctgg gtcgtcacag
     7981 tcagctactt agcctttatt ggagaagaac cgctaatcgc cggagatgat gccaaggaag
     8041 tacgctggtt cacactagaa cgtcatagta acaagatcca tttatctagc ggagaagtgg
     8101 ctatctcgct tgatttagtc actggagagt cttctggcaa agatactttg gcttttgacc
     8161 atagtcaaat catcttgaaa gcattcaacc gtgtagtcaa taaaatggaa cacgagccgc
     8221 aagttctaca agttttaggt aaagatttca cgattacgga agctcgaaaa gtatttgcta
     8281 aattcttagg gatcgattat aaaacgatcg atcattccaa ttttaagaaa gctatgctcc
     8341 aatatttcga tgagatcggt gaacgtcctg taggaatcgg ccgtccttca aaaatctatc
     8401 agctcaagcc ggggcaccat gaataaggtt gtgagcaaaa gattcagacc taatcattca
     8461 agaaaaaacg caaaaacaac tctttgtgag ttacgttttt tgtttcagaa ttttgatgta
     8521 ttcgtaaact ttctgaataa aaaattaaca atagcagaaa aagctcgtac gaactttttc
     8581 tgctattgtt ttattggttt gtcataatta gtgagctttt cttcaaaaga aaaattccta
     8641 gaaacaaaag tcattaagac tttttattaa caaggctaac cctaacgctc ctatcagttt
     8701 cattcatttt ttatcccttg ataaccgaat gtctgcccat tttttctact acttggctct
     8761 ttcttacgga attgctttct tctgctccgt cggtattgat caaaagaaat tttttattat
     8821 accattggga cttataacgc aattttgtta ttaatttatg ccagtcgtga atagaaaaat
     8881 cagcatgtgc ctcttctttt ggaaaagatt ctacaaataa gtagtcatat tgtttgacca
     8941 atttcccaga taattgttca agataatctt ctcgctggtt cttttgtttc agataaagat
     9001 ccattacttt tctcttttgt ttctgataat tgcttgcctg atctaacctg atctttctat
     9061 ggtgagcgga gcggactttc acattcaatt tccgctgtgc atgttgcagc ttttcttctg
     9121 tctggtgaaa ttttggtaaa gttacttcta gaggtcgaga tgtttggatc aatgtcttcg
     9181 gatgataggc gatcccgatc catttagcta aagattcttt ttttactggc gaaacatcac
     9241 aaagtagtgc gacataaaat tcttcattat atcgagcaga aatcgtagcg gagcgaatcg
     9301 atccttcaac tggccgatgc tgatttatct tgattagttc tttttgtttt ggtaacttta
     9361 agtagccatt ttccagatag atcgtacggg tttggttgtt ggtcgtataa gattgccaca
     9421 tacttttttt cgttttaagc tttgggaagc tggcacgtcc cttgaaataa ttgcgaaaag
     9481 cccgatccaa attaagctgt gcatttgcta aagcaagact gtctacttct tttaagaatg
     9541 ggtattcttt ttttaatgta gcgggtgtga gcgaaaaatt ggctactcct gtttgttgcc
     9601 aacttttctg ccgctttttc agcaaatggt tataggtaaa tcgagcacat ccaaaagtac
     9661 gaatcaattg ctgtttttgt tcttctgtcg gatacatacg gaatttaaaa gcttttagca
     9721 tctatgtccc tcctagttag caaccagaag agattccttt tgtcgtttta gtttttttgc
     9781 gtaatatggg acttcctgct cagttgcata gacaaaatgt tctggtgcga tttcacgaag
     9841 ttgacgtact tgtccgtcat ctggttcagg ttgatatttg attcttctgc gttttctctc
     9901 atggtctgga tctggtagag gaatagcaga taacaaactt tctgtataag gatgaacacc
     9961 gtgattatag atttcatcac ttgttcccat ctctaataac aagccgttat gcattacacc
    10021 gattcggtca ctaatatgtt taaccatcga caaatcatgt gcaataaata gataggtcaa
    10081 ttgatgttct ttttggagat cttgcaacaa attgaccact tgggcttgga tcgacacatc
    10141 tagtgcagaa attggttcat cacaaatgat aaaacgcggt tttactgcca atgctcgtgc
    10201 aataccaata cgctggcgtt gcccacctga aaattcatga ggataacgag taccgtggct
    10261 tggattcaat ccaacagttc tcaatagatc atcgactttt tttgcacgct cttcagatga
    10321 tttagctagt ccattaacat caattccttc ggcaatgata tctctgactt tcatacgagg
    10381 attcaacgaa gcgtatggat cttgaaagat catctgaaca tcatgccgga atttcgtcaa
    10441 acctttttta tctttgatct tcgttatatc ttgaccgtcg aataatatct caccatcagt
    10501 aggttcattc aagcgaatga tcgtacggcc agtagtggat tttccgcttc cagattctcc
    10561 aactaaaccg aatgtttctc cttcataaat gtgaaagctg atgtcattta cagcatgtac
    10621 ttcattcttt tttcctacat tgaaatactg tttcaatcct ttaacttcaa gtagtttatt
    10681 tggcatcagc aattcctcct tgaccgtaag tatgtttttg tttctcttta aagatagccc
    10741 agcgacgttg gatttcttct ggcggtgtca ctttgggtgc ctgcggagca agtagccaag
    10801 ttgctgcctt gtgtgttttt gatagttcaa aaaatggtgg ttcttgttct gcatcgattt
    10861 tcatcgcgaa ttcatttctt ggatagaatg cgtctccttt tggcgggttc aataaatccg
    10921 gtggagaacc aggaatagca tacaatcgat cgttcgcact atctaatgtc ggcattgaac
    10981 caagcagtcc ccaagtatag ggatgttgcg ggttgtagaa aatttcttcc gatgttccaa
    11041 cttcaacgat tcttcctcca tacatgacag cgacacgatc agcgacattc gctactacac
    11101 caaggtcatg agtgataaag ataattgatg tatcgatttt tgtctgaatt tctttcagca
    11161 gctccaaaat ctgtgcttga atagtcacat caagcgctgt tgtaggttca tcagcaatca
    11221 ggatttccgg ataacatacc agtgcaatcg cgatcacgat ccgttgacgc tgacctcctg
    11281 agaactgatg cggataactg ttgatacgtt tctcagcttc gggtattcca actaatttaa
    11341 gcaattctaa tgcagctttt tgtccctctt tttttgaaac tttattatgc tttctaaggg
    11401 actcagcgac ttgcttccca atgggcatcg tcggatctaa agaggtcatt ggatcttgga
    11461 aaatcatcgc tatttcttta ccacggattt tttgcatctc tctttctgat tttccaacga
    11521 tatcgttacc tttaaagagg atttgaccag catctatatt ggcgttgctt gacagcaagc
    11581 gcatgatgct tctggtcgtc actgattttc cacttccaga ttccccaacg atcgccaatg
    11641 tttctccttt attcaaatga aaattgacac cgcgaatcgc tcgtaccttt ccagcatacg
    11701 tatcaaaaga gatttctaag tctttcactt ctaaaatttt cgtcatcgtc ttcactcttt
    11761 cattttagga tcaaaggcat cacgcaatcc atcagcaagt aagttaaagc caatcatgat
    11821 aaccgataaa gttactgccg gaatccataa tagataaggc agatacagga atgtcttgta
    11881 cccttcgctc aacatcgttc ctaatgaagc tgacggaggc gtaagaccta aaccgataaa
    11941 gcttaaaaag gcttcgaaga aaatagcaga tggaatacta aacatcattt gaataataat
    12001 gacactagag atattcggca aaatatgttt gaatgcaatt ttcattcttg attcacctaa
    12061 tgtcaatcca gctaaaacat attcttgatc ttttaatttc attgtttgtg cacgaacgat
    12121 acgtgccatt ggtatccagt ttgtgatggc catagcagcc acgattgaaa aaattcctgg
    12181 ttcaaaaacg accaacatca aaatcatgac aacgaggttt ggaattcctg ataagatttc
    12241 caaaaaacgt tgcatcacgt tatcgacacg tccgcctaac aaaccagaaa ccaacccgta
    12301 agctacacca atcgttacat ctagtaatgc agcaataaat gcgatcagca acgagattct
    12361 cattcccata aataagcggc ttaatacatc acgtcccaaa ccgtctgttc ctaaaaagaa
    12421 atttacgttt gaagggacgt ttgcttgagc atatttatca acaagttcac caccaaccat
    12481 tgcttttcca ttcaatccat cgatattgat tcccggaata cgaggaggca aattgatata
    12541 agccacgttt tgggcagttg gatcatgagg agaaacaaag attgtgatca cagagatcaa
    12601 aatgatgacc aatagtaaaa ccattgaaat aacggcagct ttgtttttct ttaaacggcg
    12661 ccatgaatct tggataaaat tcaaagaagg tgcagtaatc tgttcacgtt cttcttcggt
    12721 atttttttgt aatggaagaa attcatccgc agggatatct gcaactgttt cataacgctt
    12781 tggtatcatt tccatttatc ctcggctccc ttctgaaaca cgaattcttg gatcaactag
    12841 accgtacaat agatccacga tcaaaataac aaaaatcagc ataaatgagt aaaggatcgt
    12901 tacagccatg atcgtaggat aatcatttgt tgtgattgac ttaacgaatt gttccccgat
    12961 accaggaata gcaaagatat tttctaccac cagtgatccg gtcatcaatg ctacagctaa
    13021 cggtcctaat aaagtcatca atggaatcaa actatttcga agaccatgtt taaaggcgat
    13081 ttcccaacgg ctcaatcctt ttgcttttgc taattccaca taatcactat gcagtacttc
    13141 caccatttcc gtacggataa accgagcaga gtcagcaagc ggcgacatgg ccaacgcaat
    13201 tgttggtaag attgtgtaca caaagccatc ccattttgcg attggcaaaa cttgtaattt
    13261 gattgcaaag atatattgta ataaaacagc aaatacaaag tttggaatgg aacgacctaa
    13321 tatcgctacg agtgtagaag atgtatctgc ccatgtattt tgcttcatgg cagagatcgt
    13381 tcctaaaatc gtccctagga ctgttccaaa gataattgcc tgtaacccta actgcaagga
    13441 tggtccgatt cttccagcta acaaatgtgc gactggctgg tttttaaatt ggaaggaaat
    13501 tccaaaatct ccttgaagca aattagaaag ataaatccca tattgtacga taactggttt
    13561 atccaaaccg acttgtttat ttagcatctc gattgtttca ggactcaaac gttcctgatt
    13621 cgtatatggc gtgcccggca gcaattgcat caagaaaaag gtgattgttg caattaacca
    13681 aagcgtgatg atcatgaaaa agacacgttt taatacatat ttgatataac tattcactaa
    13741 tgtttccccc tagcttttga cttctgacag atgtttcgct acattattag ataatgaacc
    13801 aaaaataaag gagcaatcaa atgaaaaaaa aatctatccc ctacgcagta gcttttctac
    13861 tgattctggt tattttaata aaaaatctca tcaaccattc ctttacactc attcaattat
    13921 ctaatgatct attcttatgg tcattgccgt ttctgattat cggcggtttt ttatgggtct
    13981 tttcttcggg tttcttcgat cattttcagc gatcggtcca tcttgcacga acaaggaacc
    14041 gaaagaaaaa accagaattt tcctcgctct cgtctgccag ctacggtatg tacagctttt
    14101 ggctgatcat tgccggaata ctaattgctc tttctgctat tttcatgctt ttttctttgt
    14161 taggataagt gaatatgaga acgaggttgc gacatttgcc gcagcctcgt tttttctcat
    14221 gctcacggta ttaacgatta ttcttcaacg tatgtccatt tgtaatccca tgaagcgcct
    14281 gccgcatggt gaacgatacc tttgattttt tcattcacta ggtgaccttc tgctttttgg
    14341 tacaccggaa cgacacccat atcatcagag atgatcttga ttgctttttg gaaatcatcc
    14401 caacgggctt gttcatcgcc agcgtctgtt gatgaagctg ctttaactgc ttcgtcatat
    14461 tctgcattgc tccagcgacc acggttgtat gaattacctg tgacaaacaa atcagtaaag
    14521 ctgcttgggt ctgcatagtc agcagcccat ccgcctagta ctgcgtcaaa atcacccgaa
    14581 gttgagcggt caagacgtac agagaatgga acaggtgttg gtttgactgt tacgccatcc
    14641 aagttttctt ggattgagtt ttgaacatat tcgattactt ttttcgatga atcatcatca
    14701 gaagccatca aatcaaattc taaagaatca acgcccaatt cttttttcgc tttttcccaa
    14761 tattcttttg ctttatcttt gtcataagaa actaattttc cagcttcttt tgcaaagtct
    14821 tcgttcgttg ttggactttt taccatatct gatgggatca atcctgtaga gacaactgat
    14881 ccgtcaccta gtacttgttt tactaaagct tcacgatcga tagaataaga aatagctttt
    14941 cttaggttca cattgttgaa tggtgaattc ttttcacgtt gattgaattc gatatagctt
    15001 gtccgcgctt ctttaacaga agtataagct ggatcgtttg cgttttgctg tgctaactca
    15061 ccagacaaga tcacgtcatc tgcttggcca tctttgaata agttcaatgc agtagaagat
    15121 tcttttacta cactgacttt gatttcagaa agttttactg tatctttgtc ccagtaatga
    15181 tcatttttct tataagtcca ttctgtatct gttcctggtc catcaaaatc ttctaatgtg
    15241 aatggtccat tataaacagc tttatcactt gcagatgcat aggcatcccc attttcttcc
    15301 actacggatt gtttttgtgg gaaaaatgat gggaaagcaa gcagatattg gaaatatggt
    15361 gtttgtttcg tcaatttgat ttctaattca tagtcaccaa ctgctttgat ccctagttct
    15421 gatttatctt tctttccagc tgtaatgtct tctgcatttt caactggcgc atacaggtaa
    15481 gcatattcag aagctgtagc tgggtctact gttcgttgcc agccataaac gtaatccgct
    15541 gctgtaacag gatcaccatt tgaccatttt gcgtcttctc gtaatttgat tttgtaagtt
    15601 aatccgtctt cactgacttc tgccaactcg ctagctcctg ccggctgtgg tttgctgtct
    15661 tcatcaagac ggtaaattcc ttcataaacg ttattcaatg ctgtaaaact gattgtatct
    15721 gttgcaactg ataagtcagc tgtaggcatt tcttgaggca cgactaagtt aaatacgcct
    15781 cctccatctg ctgaaccgct ttttgctgaa ctactgcttg tggctgaact gccgccataa
    15841 catccagcaa gtacaaggcc gcataccgca accaaaccaa atgtaaattt ctttttcatt
    15901 ttacgtaccc ctttattgga attttcagaa
//
LOCUS       DACEPW010000051        15620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEPW010000051 DACEPW010000000
VERSION     DACEPW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.052"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..656)
                     /locus_tag="IW819_001280"
     CDS             complement(<1..656)
                     /locus_tag="IW819_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244395.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     V"
     gene            complement(671..1555)
                     /gene="pstA"
                     /locus_tag="IW819_001281"
     CDS             complement(671..1555)
                     /gene="pstA"
                     /locus_tag="IW819_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1244396.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(1555..2475)
                     /gene="pstC"
                     /locus_tag="IW819_001282"
     CDS             complement(1555..2475)
                     /gene="pstC"
                     /locus_tag="IW819_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1244397.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(2590..3447)
                     /locus_tag="IW819_001283"
     CDS             complement(2590..3447)
                     /locus_tag="IW819_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1244398.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(3784..5232)
                     /gene="cls"
                     /locus_tag="IW819_001284"
     CDS             complement(3784..5232)
                     /gene="cls"
                     /locus_tag="IW819_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1244399.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(5384..6277)
                     /locus_tag="IW819_001285"
     CDS             complement(5384..6277)
                     /locus_tag="IW819_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1244400.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(6261..6947)
                     /gene="ftsE"
                     /locus_tag="IW819_001286"
     CDS             complement(6261..6947)
                     /gene="ftsE"
                     /locus_tag="IW819_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1244401.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(7052..8153)
                     /gene="prfB"
                     /locus_tag="IW819_001287"
     CDS             complement(join(7052..8089,8091..8153))
                     /gene="prfB"
                     /locus_tag="IW819_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1244402.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(8279..10813)
                     /gene="secA"
                     /locus_tag="IW819_001288"
     CDS             complement(8279..10813)
                     /gene="secA"
                     /locus_tag="IW819_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1244403.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(11035..11586)
                     /gene="raiA"
                     /locus_tag="IW819_001289"
     CDS             complement(11035..11586)
                     /gene="raiA"
                     /locus_tag="IW819_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1244404.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(11714..12433)
                     /locus_tag="IW819_001290"
     CDS             complement(11714..12433)
                     /locus_tag="IW819_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1244405.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(12430..13743)
                     /locus_tag="IW819_001291"
     CDS             complement(12430..13743)
                     /locus_tag="IW819_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1244406.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(13824..14144)
                     /locus_tag="IW819_001292"
     CDS             complement(13824..14144)
                     /locus_tag="IW819_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1244407.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(14252..14539)
                     /locus_tag="IW819_001293"
     CDS             complement(14252..14539)
                     /locus_tag="IW819_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244408.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            14666..15310
                     /locus_tag="IW819_001294"
     CDS             14666..15310
                     /locus_tag="IW819_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1244409.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(15369..>15620)
                     /locus_tag="IW819_001295"
     CDS             complement(15369..>15620)
                     /locus_tag="IW819_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAQ1244410.1"
                     /translation="YSKAEITESLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEV
                     KRGDAVLRLRQISYFEDGTPFEYVRTQYVGNRFEFYLEK"
BASE COUNT         4402 a         3425 c         2740 g         5053 t
ORIGIN      
        1 acgatgatga tcgtatagtc ttctttcaag tttacaagtg tttcttcaac cgttcctgtc
       61 gaaatagggt ccaaagcact tgccggctcg tctagcagca agatatcagg tttcatcgcg
      121 atcgcacgag caatgcataa acgttgctgt tgcccgccag ataaagccaa ggcacttttg
      181 tccaagctgt cttttacttg gtcccacaag gcagcttgtt tcaagcttgt ttcaacgatt
      241 tcatctaatt tctttttgtt tttttcgccg tgctgtttca aagcaaatgt aatattgtca
      301 taaatcgact tgctgaacgg attaggacgt tgaaaaacca tgccaatccg tttgcgcatc
      361 tcataaacat ccacttgagg cgcattaata tctacgcctt gatacatgat tttcccagtg
      421 attttcgtat tagcgatccc atcattcatt cgattcaacg aacgaagata cgtagatttt
      481 ccgcagccgg aaggaccaat cagtgcagtg atcttatttt tttcaaattc cagatccact
      541 cctttgattg cttcattgtc gccataccag acatgtatat catccgtata tagtgctact
      601 tctttctctt ttggcatggt gatgacattt gtttcattcc agttatattt tttcattttg
      661 cttcttctcc ttaggctgaa gtcatttttc ggtataatcg tttgccaatc gcacgagcgc
      721 cgaagttgaa cagtaaaaca gcaatgatca acacggcaga agcgccagcc gaaacagaaa
      781 caccatcagg catcgtgcct tctgtattga tcttccaaat atggacagct aatgtttcag
      841 cttgacggaa aatgctgatc gggcttgaaa cacttaatgg attccagttc gtaaaatcta
      901 acgcaggtgc actttgtcca gccgtataga tcaatgcagc tgcttcacca aaaattcttc
      961 cagaacttag gatcacacca gtcaaaatcc ctggtgtcgc ttctggaacc accactttca
     1021 tcactgtttc ccagcgtgac aaacctaagg ccaatcctgc ttctcgttgt gtgtaatgga
     1081 ccgcttgtaa agactcctca actgttctag tcaaaagagg caagttgaat acagtcaaag
     1141 ccaatgcacc agaaataatc gagaaaccat actggaattg aataacgaaa accaagaaac
     1201 cgaataaacc gacaacgaca gaaggcaacg agctcaagat ttcaatcgac gtgcgaatca
     1261 catctgttag ccagtttttt ccagcatatt ctgaaagata gatacccgca cctaaagaaa
     1321 taggaaaact gatgatcatc gtgatcaaaa gcaaataaat cgagttaaat agctggatcc
     1381 cgattccacc accttcttgg taggctttgg aaggttcggt caagaaggac catgaaatgt
     1441 gcggtattcc ccgaactaag atataaagta gtaaagctgc taaaatcaat acgatgatcc
     1501 ccgaaactat atataaaaca cctgtagcaa ttttatctga gcgttttgcg ttcattattt
     1561 cagctctcct ttcttcccaa tcatacggat cacgatatta aagaataatg acatcaatag
     1621 caagatcagc gctaatgacc ataacacatt attttcaact gttcccatga ttgtatttcc
     1681 gattcccatt gttaagatgg acgtcaatgt agaagcagga gtagtaagtc ctgttggcat
     1741 caatgctgca ttaccgatga ccatttggat agctaaggct tcaccaaacg cacgagccat
     1801 accaaaaacg actgctgtta aaatcccggg cacggctgct cgtagcacga ctttgtagat
     1861 tgtttgccat cttgtggcac caagcgctaa tgaagcttcc cgatagtgcc gtggtactgc
     1921 ctttaatgca tcaacagtca tcgttgtgac agtcggcaag atcataacga acaacacaaa
     1981 tgtaccagct aagataccaa accctgtccc gccaaaaata cttctgacaa aaggcacgat
     2041 aactgataaa ccgataaaac cataaacgac agaaggaata cctacaagca gctcgatgac
     2101 cggctgtaag atcttcgttc cgtttttagg agagatctcc gtcatgaata ctgctgcacc
     2161 aatggcaaat ggcgtggcaa cgatagcaga taaaaaggtc acaataaacg aacctgtgat
     2221 cataggcaat gcccctacca ttggtttccc atttgctccg atttggcttg gattccaatc
     2281 tgtaccaaac agaaaatcaa atacattgat cttatcagta aaaaaggtgg ccaatccttt
     2341 actagctaca aaataaaaaa tagataatac aaccaacacg atcagcgcga tacataaaaa
     2401 actgatgaat tttccacgct gttccatttt tgaacgtttt gattttgtta ataatacttt
     2461 tttcacatct tccacagatg actccccctt aatcaggttt aacatactca ctcagtgtac
     2521 aaattgaaca tcaatttggt tttaataaca tgtaaaagtt tagctaagtt tgtaaatttt
     2581 gtgtaaagat tactctttaa cgatatttcc ttgccaatca cgttcgactt tcatatgaga
     2641 aacagggata taacctagtt ctccaacgat gttatcttgt acttcatcag ataagatata
     2701 gtctaaaaat tcttttgtca gtttttttgg atgatctctt gtgtacatat gctcatatgc
     2761 ccaaatcgtc catttgttcg ttgtcacatt ttcatctgtc ggttctacac cgttaacatt
     2821 caacgtatct acatcatcgg aaacataaga aaaagcaaca tagctgattg caccgggtgt
     2881 gtcggcaacg atctggcgca ccattccgct tgaatcttgt tcttgtgcct gaacagctgt
     2941 ttctccgtcc aatacccatt tttcaaacgt gctacgggtt ccgcttcctg aagcgcggtt
     3001 caatataact actggctgat cttttccacc gacttcactc cagtttttga tctcgcctgt
     3061 aaatagtttt ttcaggtttt ccatactgat gtcttttacg ccaatatcct tgttcacaat
     3121 aggagtgatc ccaacaactg ctactcgatg atcgactaat gctgaggcat cgatccccga
     3181 tttttcttcc gcaaacaaat cagagtttcc gatgtcgact gctcctgact gtacttgaga
     3241 caaaccagtt ccgcttccgc ccccttgtac attgatgaat tttccaggat tttctgtctg
     3301 gtaagtttca gcaactgttt ctactaaagg ctgtaaagca gaagagccaa cagccgtaat
     3361 ggattctcct cgatcaatcc actttgaaca gcctgataaa agaatggcta atcctattat
     3421 tggtaagaat aatttaagtt ttttcattag aaatctcctt taatcctaaa gtatgtttat
     3481 tttatcattt tcattacttg atgaaaacga atcaaaatca ttaaataaca aaataaattc
     3541 tcataattgt aaaaaaaact atctcattaa aagtttctat gctatactgg ctttgaaatg
     3601 tgtctctttt aatagcatat attagcttat ttcttgcttt ccccaaaaaa caagagatac
     3661 cccaaagtta atttcgctca tgcgagacgc tttctccatt tctgacgtac tctcagaaat
     3721 ggccccgtta gaaaaggaca cgagatctcc atctgttgat ggttctcatg tcctttttat
     3781 gttttaaagg agcggcgaaa tcaatcttgc taatccttct ttcatcttga tgattattcc
     3841 tcggttttca tacatttctt ttgtgagatg cgtagaaatt ttcgagtctt caaaaaaagc
     3901 ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt gtattgacct caaagtccaa
     3961 gcgaaatgaa cgaacatcga tatttgctga gccaactgaa acaatgccac catcgataat
     4021 catcgtcttt gcatgaataa atccattctc atatgtttcg atccttgctc catactcgat
     4081 cagctctgca gcgaaagaat acgttgccca gtaaacgaga agatgatctg gtttgttagg
     4141 tatttggata tgaactttta cgcctgaaag aagagccaac ttcagtgctt catgaatcga
     4201 tccatccggt atgtaataag gcgtttggat caatatctcc cgtttggcta acgaaatcat
     4261 tttcaaataa gtcattttga tttgttcatg ttcggtatct ggtccgcttg tcacgatttg
     4321 aacagctata tcaccagtag tgtgaatgga cgggaaaaat ttgggagaat agcctacttc
     4381 ttttgtatgc tgagaattcc agtccatcaa aaaacggttt tgcaaggtat acaccgcttc
     4441 tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat ttctttacca atcctagata
     4501 ttcatttcca acattgaacc cacctgtata tccgattttc ccatcaataa ctacgatttt
     4561 acgatgattg cgataattga tccgaggatt aatatacgga acaaatagag gaaagaaaaa
     4621 tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag ctggaagaaa cttgtgtgga
     4681 accccatgca tcaagaagga ctctgacttt tacccctcgt cttgccgcct ctgtcaatgc
     4741 atctcgaact tcttcaccta atgcatcact gcgataaata taatactgga agtggatatg
     4801 gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat ttttcacgac catctgtaaa
     4861 taaagtgatt tcatttttag tcgtatagag cgtactttca aataccgtga gcatatagat
     4921 caattgtttc acatctactt gtccagtagg cggatgagga taaagccctc gcatcaatgc
     4981 ctgtttgtct tcttcaattt ccaggttctt accgattttc ccttggatct tcaaatcaaa
     5041 gattttctct ttggaaatcc ctcttcctaa aaaaatatat aaaataaatc ctacaactgg
     5101 aatgaacatc aaaacgagca gccatgccca tgtttgagca gtttcctttc tttctcgaaa
     5161 aacgatgata aaggaaagca aaatattcat aatcaaaagg atcgtaaaaa aattgtcaac
     5221 gataattttc acggcttcac ctcttcttct accaacttaa gaatacccaa gttgtttttt
     5281 aaagtaaagg gaaaaaagcc gcttccttct attctcctag ccaataaaaa aagaaagctg
     5341 gaacgaaact gtccaacttt ctcattttat ttatattgtg gtattagaat ttcaaaaaac
     5401 gtctcataga aatgatcgag ccaagtgaac caatgatcac accaatgccg accattaaaa
     5461 gattgatttt ccaaacgata ttttgtggac gaatcaatga ataatgggaa cgtaataatg
     5521 atggattgat caattggaaa aaccatgcat aaccaaatgt gatgatcaaa atcggaagaa
     5581 tcgcaccgat cagaccgatc cagccacctt caaggaaaaa cggccagcga atatatccat
     5641 tttttgcacc gactaaacgc atgatttgaa tttcacgctg tctagacaaa atcgtgatcc
     5701 gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag caatgccgct gcaactaatc
     5761 cccatgtccg aactgcacca gcgatttgga aaatccgatc tgaggaagct ccaccgtaat
     5821 cagaacgaga aacattcttc aaatctgctg cttcttctgc tacttgtttt gtataagaag
     5881 gatcagtagc acttactaca tacacatcat ataacgggtt attgtcacct tcaaacaatt
     5941 tccaggaatc acccatttcg ttttggattt ttttcaattc ctgttctttg cttgaaaatt
     6001 caactttttt aacatgatct aagtcattca attctttttc tagctttttc atctcttcgt
     6061 cttttgtacc gatatctacg aatacagata catcaacgtt tccttctata tcctgagcta
     6121 atttagttgc attcatgata actgccatga atacaccgac taaagtcaac gtgatcgtta
     6181 ccgcactgac tgaagcaact gtcatccagc cgttacgttt caaactcttg atactttcta
     6241 ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt ccccttccgc ttgatctcga
     6301 ataatgcggc cgttttcgat agcaattact cggtgccgaa tcgtatttac gatcgtactg
     6361 ttatgtgtag ccatcacgac agtagttcct tgggcattga tccgatctaa taatttcatg
     6421 atttcccatg agttttctgg atccagattt cctgtcggtt catccgcgat aagtacttta
     6481 ggcgtattca cgattgcccg agcaatagag acacgttgct gttccccacc ggataattcg
     6541 ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt ccagcacttc cattactcgt
     6601 tttttgatat ctcgaggttt tctgccgata acttgcatag cgtacgcaac attttcatat
     6661 actgtttttc ttggtaataa tttatagtct tggaacacca cgccgatttc tctgcgtaga
     6721 taaggaactt cgcggttttt gattgctatt agatcatgac ctgcgacgtt caaagttcct
     6781 tttgaagctt tttcttctcg atacatcaat ttgatgaatg tagatttacc ggctccagac
     6841 ggaccaacca catagacaaa ctctccttgt tcgatgacaa tcgagagatt gcggatagcg
     6901 gtcgtaccat ttgagtactt cttcattaca tccttcattt caatcatggc gttctctcca
     6961 tatctttttc agtctttagc tattatagca tggcaatgtt tcaacatgct tgcaaaatct
     7021 tacaatttta tttcaattta acaatgagtt cttattgtag atgcattttt aaatagccgt
     7081 caataaataa atcaaggtca ccgtccataa ctgcttggac atttcctgtc tctacgtttg
     7141 tccgatggtc ttttaccata gaatagggat ggaaaacata agaacggatc tgtgaacccc
     7201 agccgatttc tttttgttct cctcgaagtg cagctgcttc ttgttctttc ttttccactt
     7261 ctaattgata aagcttagct tttaacatcc ccatcgcttg ctcacggttt ttcaattgtg
     7321 agcgttgtgc ctgactagct actactgttc ctgtaggcaa gtgagtgatc cgtacagcag
     7381 attctgtttt attgatgtgc tgaccacctg caccacttgc tcggtacgta tcgattttca
     7441 aatcatctgg attgatttct atatcgatcg tttcatcaag ctctggcatc acatctaccg
     7501 aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg cgaaatccgt acaaggcgat
     7561 gaacaccttt ttctgatttt agataaccaa aagcgttatg tcctttaatc agcagggtca
     7621 cactcttgat ccctgcttca tcacctgctt gataatctag tgtttctact tgatagccat
     7681 gctgctctgc ccaacgtgta tacatacgta aaagcatgct gccccagtct tgggactccg
     7741 ttccgccagc ccctggatgc aattcgacga tcgcattgtt tcgatcataa ggtccgtcta
     7801 acagcataga taattcatac gtcttcattt tttcatctaa tgcattgata cgtgtttctg
     7861 cttcttcttg cattgctgca tcaggttctt cttgcagcat ctccaccatc aattcgattt
     7921 cttctagttc ttcggcaaga gaatggaatt ggtcataggt ttctttatta gcgttatttt
     7981 cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa accaggttca gccatttttg
     8041 cttctgcttc cgcgatgtct tcttccagct gatctaagtc aaagagacct cctgaaactt
     8101 gtgacctttt cctgcatttc tgtcaggaga tttcgaattt cggatatttc catgtttttt
     8161 ccttctttca ctatactttt ttcattatat ctagtctaac agaatcagca aataacagaa
     8221 agaaggtgag acgaattctt aaaaaaagaa tttgtctcac caatcctgct gtcagaaatt
     8281 agtttgcatt ttttccatga cagttcttat attttttccc gcttccacaa ggacaaggat
     8341 catttcttcc gaccttgttc acatggattg gctgtttttt cgcgctagta ttactatttt
     8401 ctttggcatc tccgtcttct gagtgagaag ctgttccttg agaaacctgt tcacgctgta
     8461 cgttttgacg gatctcagct ttcataaaga ttcgagtgac ttcatactcg atggcgccta
     8521 ccatatcttc aaacatctta tagccttctg tttgatattc gactaatgga ttgttctgtc
     8581 cataagcacg taagccgatt gactgacgta attgatccat tgcatcaata tggtctgtcc
     8641 atttagagtc cacaacacgc aagatcacga ctttttcaaa ttccaacagt tgctctggac
     8701 catttagttg agccgctttg gtattaaata cttcttgtgc tcgttcgttt agatactctt
     8761 tgatctcctg agatgttttt ccttcgatat ctgcaagagt aatactatct tcatggacaa
     8821 gagtactgcc ggcaaaatcg atgatacttt ccaaattcca attttctttc tctaattgtg
     8881 tatgcgcatc tactacacga ctgattgtcc gtttgaccat gttcattagt ggcttggata
     8941 agcttttttc ttccatgatt acttcttgtc gctgtttata gattacttca cgttgttcac
     9001 gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc atagttattt ccttctacac
     9061 gtttttgtgc agattctact tgtttcgtca gcatcttgct ttggataaca gcatcttcct
     9121 caccgatctt catccgatct aagaaagctt tgattcgttc tgaaccgaat cgtttcatca
     9181 aatcatcttc caatgacaag tagaattgtg acataccagg gtctccttga cgtcctgcac
     9241 gtcctcgaag ctggttatcg atacgacgag attcatgtcg ttctgtcccg ataactgcta
     9301 gaccaccaag ttctcttacc ccaagtccta acttgatatc tgttccccga ccagccatgt
     9361 tggtagcaat cgtcacagca cctttttgac cagcattcat gatgatctct gcttctttaa
     9421 agtgattttt cgcattcagc acttcatgag gaatacgttc ttgattcagc atatctgata
     9481 ataactcaga tgtttcaacc gcaacagttc cgactaacac aggttgacct ttgcggtagc
     9541 gatctttgat atcttgcacg acagcgtgga acttgctttg taatgttggg taaagtaaat
     9601 ctggacgatc atcacggata accggacgat ttgtcggaat ctggtatact tggatgttat
     9661 aaatttctct gaattcttct tcttctgttt tagctgtacc agtcattccc gcaagtttct
     9721 tgtacatacg gaaatagttc tggaaagtga tcgtcgccat ggttttcgtt tcgtcttcga
     9781 tctctacgcc ttctttagct tcgattgctt ggtgaagtcc atctgaataa cggcgtccat
     9841 ccatgatccg accagtgaac tggtcaacaa tcagtacttt tccgtcttgg acaacataat
     9901 cgatatctaa aagcatgata tagttggctc tcaatgcttg atccaagtga tgggtcaatg
     9961 cagtattttc aatgtcgtat aagttatcca aaccgaatgt ttcttctgct ttttcgatcc
    10021 ctgcttcggt taaactgatt gtttttgatt gtacatcgat tttataatct tcttcttctt
    10081 tcaaacgttt cacgaagttg tctgctcttg tatataaagc tgtggatttt tctgcttgcc
    10141 ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat tgagtcaact tcatcgacaa
    10201 tggcataatt cagcggacgt tgtaccattt gatggcggta aacgaccata ttgtctctca
    10261 agtagtcaaa tcctaattca ttattcgtac tataagtgat atcacaatta taagcctcgc
    10321 gtttttcatc cgaagatttt gagttgatat tcaaccccac tgttaatcct aagaaattat
    10381 aaagttcgcc catttccgtc gagtcacggg ttgctaaata ttcattgacg gtaactacgt
    10441 gtaccccttc gccagtcaga gcatttagat atactggcat cgttgcggtc aaggttttac
    10501 cttcaccagt acgcatttca gggatatttc catcgtgaag cacgattcca cccatcaatt
    10561 gtacgtggta tggatataac cccaatacac gttttgctgc ttctcggacg actgcaaaag
    10621 cttccggaag taattgatct aatgtttctc ctttttggta tctgcccttg aattcagcag
    10681 tttttgcctg taattgttca tcagttaatg ctgccatttg gtcagcgtat gcttcaactt
    10741 tttttgcaat gctatctaaa cgttttagtt ctttcttatc gttttcgata agtgaacgaa
    10801 tcaaattggc catgcgttgt gtaatctcct tttccttctg tgtattatcc agactatgta
    10861 gactttactt ttcattttat cattagttat agataattga aatatcttaa gcgtattaaa
    10921 gtaagaactt cataatttat tcatatttca tctaaaagag gaggaaactc aaaaaaactt
    10981 gcggcagctg tcagtagctg ctgcaagttt tcgttgttat ttaagtaagg ctgatcagtc
    11041 tgtttcaatc agtccgtatt ttccatcttt acggcggtaa acgatacttg taccatttgt
    11101 ttctgcatct tcaaaaataa agaagttatg tcccagcatg ttcatttgta agactgcttc
    11161 ttcactatcc attggtttca atgaaaggcg tttcgtacgt acgatatcta attcagaatc
    11221 attttcgttt tcgtcctctt cattgaataa agcagcagca tctgcagtat tcatgcccgt
    11281 ttcacgggat ttacgattaa tttttgtttt gaatttgcgg atttggcgtt ccaatttatc
    11341 aacaaccaaa tcgatacttg cgtataaatc tggagaagtt tcttcggcac gtaatacaag
    11401 ataaggtaat gggatcgtta cttccacttt tgctgttttt tctgtataaa cttttaagtt
    11461 cacatgcgcc gttgcatcgg gtgcgtcact gaaatagcgt tctagtttgc ctactttttt
    11521 ctcaacgtag tcgcgaatcg cttcagttac ttcgatattt tcgccgcgta cattatatct
    11581 aaacataaaa attacccctt tcgtctacca caatcaagaa ggagaaggac cactctttcc
    11641 ctcttcttaa ttctattata tcgaactcta ggttatttgc aaattaaatc gctatcaaat
    11701 ttccacatct aattcatctc gccaaagaaa tggttcggat gattttcggc tgtgcgacct
    11761 gcaaacaatc tgctgcgtgg aaaagagtct gtcctgttgt atagacatca tcaataatca
    11821 atattttttt attttgtatc ttagaaaagg tgactgcaag ttcaaaaggt tgaggcatct
    11881 ccaatctttc ctttcgcgtg ttttttgctt gagccagcag atgcttctct cttttgagta
    11941 gcatctgaaa aggaattctt gctgctttca acatctcgct tacttgattg aatcctcttt
    12001 ctgttcgcct tttttcagat aaaggtattg gacaatagat gtatgtcggg tattcttttt
    12061 ttatctgcgt ccatttatat gcaaaggtgc ctgctagtcg gatatctccc ataaacttgt
    12121 attgattgag ccaggccttc atattttcat cgtacacaaa aaaggcacgg tgctgaaaat
    12181 cataggtcgg atatttcttc ttccattctt gacagtcact gcagtaaacg ggaatagact
    12241 ttactttctt tttcgtactc tctagattga aatggattgg tttttgacag ccagaacagc
    12301 ctcttgtttc caataaatga aattgtgcgc gacaaactga acaagtctga ggtaatctgt
    12361 atttcgaaac accaataatc tcgctgaatg ttaaattttg gaagatatct ttactgcagc
    12421 aactgcacct cacagcatct tcttcccttc ttcattcatt tctttgattt cctgacaagc
    12481 ttttcgaata gctgcagttt tttcttcata gaaaaagtat acttgactgt tcgtataagg
    12541 ccctttacga tcggctcttc ctgcgatttg taccaaagaa gatttagtaa aaaccggatg
    12601 atccgctcct aaaacgataa cggatattct ttcaaatgtg actccacgtt caaggatcat
    12661 cgtggttaac agtatatcat attttgcttc tcgcattttc tggacttttt ccgcccgctg
    12721 attgtctctt gaagaaacat ctgtgatctc atatctcgga agagcctctt gaagtgtggc
    12781 tttcaaacga tgcatcatcg tgatacttgg acaaaataaa aggatatggt tttctttaat
    12841 taattgatga atgatccgca ccaaagattt tatcttcttc ttgttcttgc atttttgtcg
    12901 ccaattattc atgaaaacca agacgggttc aggaagcaaa cgccgatgaa agcgtaaagc
    12961 aagtttgtgg atgtcaaacg tctgcttgat ttcctttagt aactttttat caggtgtggc
    13021 acttaagtag atgaatcgtc cgtctggttt aatcgctgtt tgcacagcat gagctaatcc
    13081 tgcatccccg ttataaggaa acgcatcgat ctcatcgatc actattaaat caaaggaccg
    13141 ataaaaacgg tatagctgat gaatcgtaca gataacaaaa gaagtaaagg tataagccgt
    13201 tgctgtattc ccatggagca acgtgatttt ttcttctgga aaaacataac gatagcgtaa
    13261 aaacaattca ttacaaacat cgactcgcgg tgtacaaatc gctactcttc ctccagcttt
    13321 taaagtttcc actaaaatag gataaagcat ctctgttttc cctgcacctg ttacagccca
    13381 aataagatga tgtctttttt ctgaatggca aagtattctc tttgagattt ccgtttgttt
    13441 ttcagtaagc tgtcctgtcc aaaaaaagac gacttctcga ggttttgctt caggctgatc
    13501 aaacaaatat aaatcttgtt gagtgtcgca tcgccctaaa tatagacaat gtgtacaaaa
    13561 ataaccgtaa tctgcaagct gtccatcaat gacggacgtc ctcattccac agcgatgaca
    13621 aagaatccag ttgcccaaac gttccactgc tgcgatcttc tcataatttg gtaaatttac
    13681 ctcctcacag tctattgctt caatatctct cgtcagaagc cttctacccc aaaaatgatt
    13741 catattcttc gccctaaaaa aatatacgaa aaaacaagcg atctattttt tggatcgctt
    13801 gttcgatagt tatactgttc tatttattca cctatgagcg tgattgcttg atctaacact
    13861 ttttcgccat tcatcatacc ataatcagcc atattgatga catctaaagg aatgcctttt
    13921 ggttccagac gtttctcaaa atcagctttc ataaagcgaa cttgtgggcc taataacaac
    13981 acatccacgt ctttatttgc aaggttgtta tctgcgtcag aagcggacac agcgaaaata
    14041 tccgagtcga ttccttttgc ttcagcagct ttttgcattt ttgttactag taaacttgtg
    14101 ctcataccag ccgaacatac aagcatgatt gtttttttag ccatactttt ctctcctttt
    14161 taaaacaaac tatgcaaacg ttttcttcgc gtctgttact ctattatata aaaagaaaga
    14221 aatttgtcaa cgttttctaa acgcggttta tttaatcaaa tagccaacta gatttcacta
    14281 atttattgaa cacgacatat tctttgtcca aattgatgac aactacttca tagcctgcat
    14341 ttatccagcc ataggcacct ttttcagggt gtttataatc atttgcccac caatctgtac
    14401 tttcccttgc tgttttggga agtccgttac tgggaaacag cagttcgtca aattgggtaa
    14461 aggtcaacgt tacttgagag ccgccattgt tcttcaaata atgcgtaatt gcatcgtact
    14521 ttttcattcg ttcctgcatc cccagatcca cctgcctttg tctttattat cgcatactac
    14581 agcaattgcg caaccgttac agtgttgaaa caacctagaa ctttatttat actgaataaa
    14641 gaaaatgaaa aaaagaggtg acaagatgat cgagcgctat cgtacaataa aagaaaatgg
    14701 acaaagtgag attgagatca aaaaatcacg ctttatctgt tctcttagac gaatagaatc
    14761 agaggaccaa gcaaaaagtt tcattcaaga aataaaaaaa gaacattgga aagcgaacca
    14821 taactgcagc gccttcatca ttggagaaaa aaatgaaatc cagcgaagca gcgatgatgg
    14881 agaaccaagt ggaacggcgg gcgtaccgat gttggaagtc ctcaaaaaaa atcaactgat
    14941 caatgtagct gcagtagtca ctcgatattt tggaggaacg aaactcggca caggcggatt
    15001 aatacgagca tacactcatg cggtctctca agcattgtct gacattggaa tcgtagaagg
    15061 taccttgcaa caggagatct ctgtgatgat cacgtatccg caactaggaa aattacagaa
    15121 ttatcttgaa ctccaacatt tttttataaa agaaatccaa tatactgatc aagttagcgt
    15181 catctgtgct gtagatgaaa aaagtgctga ccactttaaa gaatccatca ttgagctgct
    15241 gaacggacaa gtccaatttg aagagggttc tgtttcttat cacgagcaac tgataactga
    15301 tcatatataa aaacaggtga ttctgattct cttttatttc taataaggaa tcagggatca
    15361 cctgtttttt atttttccaa ataaaactca aaacgatttc ctacgtattg cgtacgcaca
    15421 tactcaaacg gcgtaccgtc ttcaaaataa gaaatctggc gcaaacgaag aactgcatct
    15481 cctcgtttta cctctaaata gtcagcaatt ttctcagaag ccaacacagc agtaatcgtt
    15541 tgattcgctg gaccgatctt tttcccgcct ttttcttcta gcgtgcgata caacgattca
    15601 gtaatctctg ctttgctgta
//
LOCUS       DACEPW010000052        15607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEPW010000052 DACEPW010000000
VERSION     DACEPW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.053"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..1528)
                     /locus_tag="IW819_001296"
                     /pseudo
     CDS             complement(1..1528)
                     /locus_tag="IW819_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            1654..2319
                     /locus_tag="IW819_001297"
     CDS             1654..2319
                     /locus_tag="IW819_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1244411.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(2716..4749)
                     /gene="brxL"
                     /locus_tag="IW819_001298"
     CDS             complement(2716..4749)
                     /gene="brxL"
                     /locus_tag="IW819_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HAQ1244412.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(4867..7389)
                     /gene="pglZ"
                     /locus_tag="IW819_001299"
     CDS             complement(4867..7389)
                     /gene="pglZ"
                     /locus_tag="IW819_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HAQ1244413.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(7505..11137)
                     /gene="pglX"
                     /locus_tag="IW819_001300"
     CDS             complement(7505..11137)
                     /gene="pglX"
                     /locus_tag="IW819_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HAQ1244414.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     NVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(11201..14872)
                     /gene="brxC"
                     /locus_tag="IW819_001301"
     CDS             complement(11201..14872)
                     /gene="brxC"
                     /locus_tag="IW819_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HAQ1244415.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKFTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(14897..15472)
                     /locus_tag="IW819_001302"
     CDS             complement(14897..15472)
                     /locus_tag="IW819_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HAQ1244416.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(15472..>15607)
                     /locus_tag="IW819_001303"
     CDS             complement(15472..>15607)
                     /locus_tag="IW819_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1819 domain-containing protein"
                     /protein_id="HAQ1244417.1"
                     /translation="IETGLVRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL
                     "
BASE COUNT         4850 a         2979 c         2178 g         5600 t
ORIGIN      
        1 aatacgtact gtttttcaat gtcagtattt caacaaccgg ataatgttcg atccagggga
       61 tagaaggaat aaatgtataa atgggttgat tgttttttat aaatagaaat ttcggttctt
      121 tttcactgat ttcttgataa tattctgtta tttgcgccag gtcatttttt aaatctgtat
      181 gtctatatcg ttcagggatc aatataacag ggtgcgtttc tttttcggtt atttggatca
      241 accttccaga gacatcaatc aaaggaaatt tcttaagata attcagattt atttttatcc
      301 ctctgccaaa ctgttcattt tcttcattca agtaatctga tacattgact aatatacttc
      361 cgtttttatt caaagtttca tataattcct gatcggctgc tgatgcaaaa cgattggaat
      421 aaataaaatc cacgtggttt ttcccgatgg ttactggata aaatacatga tagtcctcac
      481 cagcgatttc ttttggtgtt tcatgggaaa cagctaactg tgaagaagaa aaaacgatag
      541 ttgctaattc gacattggca acggtcaaaa gaagagaaaa caacaatcca aatgtaaaaa
      601 gaatgtattg gatatagttg aacgcttctc ttaacgaagc gattttttca actaataaaa
      661 gagaaatcaa agagataccc tatattccgc tgaacaagaa aacgtacatc tggaacaaat
      721 aaagaaattg ctccttataa tacataaaaa taaaaccacc aatgattcct gcaattatga
      781 atgtaataat gattttcttt ttccccaata aacaccacaa tacttgatgt aatgaaaagc
      841 cattattcaa caataaactg atttctttat tcatcaataa gaaaaaggat gataacaaaa
      901 ggaataaaag aatcaatcca atagttaaat acactcgata attatatacg tctgtacttg
      961 aaataaccga atacgaatag ctattttcaa agtcttcaaa aatattagtg gcataatcag
     1021 tgtgaaatgc ttgattgaat cgttccacaa atagtaaaat gaaccgttca taggcttctt
     1081 tttctctcgt catcaagaaa aattcacctt caaaatcttt tattttgatc gattcttcaa
     1141 tggacgttac gcgaaaggag gtattagaat agggtacctt ctcgtgagaa gatttgacat
     1201 ccccttgata atacaatgtc atttcaagct ttggctgtat cttgaagttc caagatgtat
     1261 ctacttcatt ggattcattt actgtttttt tcatataaga aagctcagtt tctgatgaga
     1321 cattttcaaa taccatgatt agctgctcat attcttcttg cccaataaaa tgttgtggca
     1381 atcgaatcgg agtataagta tctgtttgat ggaaaaattc atttgtccga tagagtacct
     1441 gtttatttat aataaccaag gcaaaaccac tcatgatacc ggtcactatg attgtgagta
     1501 aaactgtgaa cttttttttg attatcacca ctctccctta cttaaatgat tctttgataa
     1561 aatcataccg tttttcatca aaaaaacgta tctatcaaat gtataggtca tgtagaataa
     1621 atctatatac tgtagaatcg aaaggagaag aagatgaaca tcggagaaaa aatacgaact
     1681 taccgcagaa attgtaagct tacgcaaaaa gaattaggcg aaaaaatcgg tgtttccgat
     1741 aaaacaatct ctagttggga aaattctcgt acaatgccag accttgaaat gctgtcactt
     1801 ttacatcaga ctttaggtct gccgattgat tttccttcta ttgaatcagc taccgaatca
     1861 atagaaccaa cattgccgga tgaacctctt gttatcaaga aatcttattc atcaaaacaa
     1921 cccttctcac tcaaaaagac actatctttc tttctgctcg tcttttgttt ggtctatctt
     1981 tttcttatca attatcaata taaaaatgaa acggttgatc gattcaatcc ttttttgaaa
     2041 ccacaaatcg gctataccac actccctgta ggatacgtgg aaaattccat cgaacattgg
     2101 gtatgtaatg atatctttgt tcatggtaca tggctgtctt ttcacgctgg agaggtagaa
     2161 aaaggtaaga atcatgctgt aatcaaacac aaaggaaaac acgtcatgaa cgttcgcctg
     2221 ctcacttggg aagccattcc ctcagattac caagaaatcc tcatccaaga ctatgacgat
     2281 gccttaaaaa gaagccctca ggagccagat aggcattaag taggagatga acgtagagat
     2341 agaaaaaatc aatgcacatg atcttatcaa aaaaaaacag gggtacaaat taattgaaaa
     2401 agctttgaga tgaaagttcc taaataaaaa agttcagaat gataggtaaa aaaatgacta
     2461 actattctag tattaggcta tttgtattat tcttctgtta tttttatatc aaaaaatcca
     2521 gaaataacta aatataggta tttctggctt atagtataat atttacgcca cagcccccaa
     2581 tgattctttt tggaatcatg atcaaggacg gacatacaca aaaatatttt gcccattcga
     2641 tccgaataga ttaacgacta atctagtaag taatatagat tggtacgtag tcaaaccagc
     2701 aaatggaaaa gttgtctatt ccaccccaag ggctttaaag acagcatctt ccggcgtttg
     2761 ataaaaaatc aaattaaagg tacccattaa ttctgggggt acagttccaa tatcagcagc
     2821 gcttatcata ggtacaagta cttttttcgc tccggcatct tttgctactt gtaaacagtt
     2881 agctaaatta tctaccttaa tcatagaacc accaattgta atatccccta aaataactaa
     2941 acttggtaat acaggtttat ttaaggaaac tgagcataaa gctattatag ttgataacga
     3001 taatgttttt gtcataccaa tgccattcat atcttgaaca tgaacgacaa aatcttttgt
     3061 cgtcaaacta atagactgac taacacttcc ataattagct cttaaatatc ggtatgccga
     3121 atccatcgct tctcttgctc cagcatctga attgatccca gtacgttcaa actttccatt
     3181 tcccgaagtc atttgcgttt ccaatttata ggtacctaat tgcccagttt tgccataaga
     3241 taccgtgtac aaatgccctg gcttccctaa cccttctgga atcaaagttc ctccaccttg
     3301 ttcaggaaca gaaacatact cttctcgctc ttcttctaaa tcaatgtatg aaaaattgac
     3361 atcataaaat tccattccac caattttctt taattgttct tttacacgac gacgcatttc
     3421 aagagcaaat cttagtattt cttcgacatc acttttttca aatctaccat caggatacaa
     3481 cagtttcatc atcccagata caatccgacg aactgcaatc gtatcccgct gatttaattg
     3541 agaaccaagt ttgaaatatt tatcaaaact atcgccaaaa ctaacttttc gaagctcacg
     3601 catgacttca gaaaaataat ctgtaatgaa gccatagtca tttgtaaaaa aatctggtcg
     3661 atatttagga atttcccaac ctggtaaata acaatgcatc cgatccaaaa aggctgaatc
     3721 cgtccccata acctccggaa atggctcaaa taaattcgaa gttttcaata ttacatcaac
     3781 gctttgatta atatttccta caaaagccat tgatgctgaa gcattctttt cttctttgcc
     3841 tctagcaaaa gatcctgaag ccatgtaatc tttcatgatt tgaacgccat cattatcctt
     3901 aaatttaatc ccagcaactt cgtcaaaagc aacacagtcc cacataccta caagtcccat
     3961 tgtacgacta gccatattat agaaaaggtt tgctactgtt gtttgtccac ctgaaacaag
     4021 gattgagttt ggtgaaattt ctttatatac atgcgattta cctgtaccac gtggtcctag
     4081 ttcacagaga ttaaaattat tttcaattaa tggaataagt cttgctaaat gaagccattt
     4141 taccctttta ttaaattgtg taggttccat accaatggac cgtagaagta agtcaatcca
     4201 ttcatttgta gtaaactcct tgcgagccat cttgtaagcc tcaatgtcta aaccaggcat
     4261 ttggattgga tcaacctttt ttgcaataaa agggctattc caacgatcat tctcatcata
     4321 ccaatattcc atcgaaacaa tacaccagat ccccccacct aataatcgat caaatttttt
     4381 cggatactct tcactaattt gaacattttt taatcccagg ttagagaatt ctgcttcata
     4441 acaatctttc ttttcattta aatgtacagt tactttatca ataactgtat aaccacccaa
     4501 ttcacgaatt tttgctttta ctttttctgc ttcatcagga cgcacatagt ttcgagcaag
     4561 aatattttta acattttcca cacctttttc aaccaatgtt tcatccaatg acgaagcata
     4621 cataccaagt aaatattcca atacataaac aggtacattc gcaccttctt taattttctt
     4681 tgttaaatct tttctgacaa tctttccggg aaagaattca gataacttta aaatttcctg
     4741 ttgttccact agaattcctc catgtgttca gtcattataa tagcaattaa agtatatcat
     4801 acttaaccag taggcttgat aatacaaaag aaaaagtatc cacaaaaaat tgtggatacc
     4861 tcgtttttat atatcaaaat caaatgattc tgcaaaagga ttatctataa tgaattttat
     4921 tcgctcggtc aaatcatttt tttgattttt tatcactaaa taataagtat cttcaactgt
     4981 atatcgacga tttacaaatt caaaaacaaa tttagtaaac cgctgatttg catcttctgt
     5041 ttttgaatcc gcaacataga tttcttcatt tgagatacgt tcaccatatt catcttcaaa
     5101 ataaagccag aaagatgcgg ggtcataaac atcactaatt gcattttttt gatagaattc
     5161 taacgtggtt gataatcctg taattctgcg gttgctagtc attaattcaa ttgctactgg
     5221 ttcctttgca ttgctacctt tggctaccgt aatttctaga acagggacta gcatttcttg
     5281 aatagatgac cctccatgaa cgtagctttg tcccccgcca gctttcttaa aaatacttga
     5341 agtaatcggg acacaaatgt ttctagtgtc atcatttcct aaagaatctc ccaaagagta
     5401 atttttcgta ccaattattt cgtattcttc ttcactaaga ataaaacgac gttctacacg
     5461 atctttttca tttctagatg gattttcaat cttatctact tcttcaacgt ggcttctttt
     5521 ataaataaat ccatgatcag ccgtaactat aaaacgatag atactgccat cattatgcaa
     5581 gcgattaata agtcgggaaa tttcgcttat cgctaaatca caggcctcaa aaactcgatt
     5641 ttcattcgag tgatcacctg ttctatccac ttgatcgtga taaatataaa tgactgattt
     5701 gccattaaat acttctcgta cctcagcctt attcatctta accacatcat cataatttaa
     5761 agctacagca tttgcatctc gttgctggag aatcgcattt cgattggata aaccattcgt
     5821 ttttttccca tcaatcagta cttctgttcc ttcattgtaa gttagttgat tgtttggtaa
     5881 taaggcggct ttaccgaatt cagtaactga aggtaataca gaaagaaagc tagccatgtt
     5941 tgttgaatac ttcttttctc taactaactt acgttctaaa ttcatcccta actcatatcg
     6001 aaaagcatct gaaataataa ctacagtttt aacagagcgt ttcattacag cttgattata
     6061 aaattttcga atggatggac gattcttaaa ttcaaaagtt tgattccaaa tttgtcctga
     6121 ttgatcaaga aacttcgaat aatttgcttc aactaatcga tacaattggc catatttttg
     6181 cttttcttct atatgatcac tattccaaat aaatttccga taatgcgtat caacccaata
     6241 catttccttc tcatattgag ttagcaaatt atccatgttc atcggcaagc acaatctgtt
     6301 tttatccaca agataataag catgtttcaa taattgatac tcataaatat aatccgatgc
     6361 gaaatgcatt ttttcgcgct gctttaaact atcaaaaatt gtttgtcctg caattttcgt
     6421 tgtagtgtca gaagccttta atcgttctgc ataaaacatt aatatttttg catgaatctc
     6481 agaaaatacg tctgcttcaa ttagatcatc aatttctata ctgtctaata atccatcacc
     6541 atcaatagcc tgatatactt ttttagatag ctgttcaaag ctttccatgt aacgtgtatc
     6601 attcataaac tgatccatga aagtaataat gtttgctttc aaatgtagta tttcaaatcc
     6661 tttgaacttc ttcggcatct cttgatcaat ttgataataa aaatagttca aataaagaca
     6721 catcgtcagc ttttccaatg ttggctggct atccgtaaag ccaaatttca tttgaataaa
     6781 ttcccaaaat ttagctgtta actgatactt atcaagttcc cttaaaggat tctccctact
     6841 ttctgtaaag ctttttagaa ctacacgcag taaatcattt aatcgaatac ttttcgattt
     6901 caacaaaaca gccattatca ttaattcaat cgtttctgat ttattaattg tcatatgcgt
     6961 actaaattca ccaaaacttc tcatgcgatc ctttgcatta aagaaattac gatactgttt
     7021 aactgtctga tgaaatctaa gtggaatagc caattcattc atgataatcg ctaagcggtc
     7081 tgcactaaat tcctctgaat aattcaatac actcaacaaa tgattatctt catctttatc
     7141 atcccaacga ggaaatggcg catagattaa atagttagtt tcgggatctt catattccaa
     7201 taaacgctta gttttaaatt gtgcgtcctt ttctaatcga tagatttttg cattttgaat
     7261 gaccaatgag tcaatttctt caagaaaatc ctgatttgaa tcaaaccaaa aaatgatttt
     7321 cctacatcca taattaggaa aacgttgatt taatttttct tcaatttgtt gcgtatttat
     7381 atctgtcatc aatctcacct aatcgttctc tttctatacc ccttatattg acataatttg
     7441 gataatttcg cacgcaattt aagaataaaa aagaaagttg ttagaaaaca actttcttag
     7501 ataactattt ttttggttct ttgacccctt ttgcaaaaat ttggaatttc tcgccggttt
     7561 taggatcacg ctgcaactta tcataattga caattacacc atcatctaag tctaattcaa
     7621 ttcgttttaa tgctaagtga cccaacttct cgtcatactc acgaacttct tttaattgct
     7681 tctgaatctt tacaatttct tttgtcattg catttctttc ttttgggtta tcacttgttg
     7741 aaatttgttg ctgacgcaat tcgattcgat tgtcatacgc tttttgtagt tcgtgtaggt
     7801 attcagtacg tacttttcct aattgatctg gtgtatagcg atgcaagtac attaacgctt
     7861 taaagccatt ttgttttcct gagtccaatt gccaataaat tggtcgattg gagtatgttt
     7921 ttaaatggtc tttgtaaaaa tcttgtacga agtaacgacg aataatttct cgacttgtcc
     7981 ctttgccacc taaagtgtca gcgataaact ggagattttc ttcaagggtt tctttaccaa
     8041 aagtaatctc aacgaaatca atgaaacgat tgacaatgtc aatttggcta tccttgaaat
     8101 actcatcatc ggtgatgagt aaaacattat cagtatcagg tttaaatttt tgatagcggg
     8161 aaggatccca ttcgccgcca gcgaagatga gaccatcttc gtctagggag taacgaccga
     8221 agatacaacc tattgaataa gaaataaaat ccttaatttc cctcgcctta tcaggtaacc
     8281 ttgcagagat atattcatct tgaacctctt ttgttaattc tttttcaaga gaataatgat
     8341 taatgaatat gtcgttcagt tcctcttcat tcgattttag agtattaaat tgattaacca
     8401 actgcctact tagacagtta aatgcctctg agagatcttt tttaaacata taacttaaca
     8461 aaatgtttct tctaaactcg tatgacgttt caagagcatt atagtctttt ctagctatat
     8521 cgatattttc atccactttt tctcttattt tttttaattt tctctcagat gtaactttaa
     8581 gaaaaggtag ttttcccatt ggtccttcat ggtaatccaa ggtgggagaa ataaaagtta
     8641 aaaagtgatt aactaattta gagtttacat agccaaatat ttcttcaaag tcagttatat
     8701 cctttgagaa cagaacagat cctttagttt caaaaattgc ctgattctgc aatctaaaag
     8761 atacttgtcc agatgaaagt gtcgaccaag taattccctt tttaaaatag taattaagat
     8821 tttgtggtct agattttaac ttcccatttt cattaacata attttttatt tcatctccat
     8881 cattctccca gttaaccacg tattcattgt ttccatacca ttttctaaaa cgaccacctt
     8941 tagttgtagg aaaccatttt tttctagact ttttagcgtc ctctaatgtt ttagcatcaa
     9001 actttatttt gttaaattct acttcaaacc aataccgcaa aaatttattg ttatttccgg
     9061 ttgccaaccc ttgttttgca tctgcaacat cgttagttaa ttttccttga gcaaaatcag
     9121 tgattacgtt atcgcttgcc cagtaagcaa tcggcattcc tgggattttt ttgaagttgt
     9181 cttgattagt actataaaaa taatcgcatt caccatgctg atgaattgct tctaacgttt
     9241 taattttttg aacttccatt ccacctttaa aatctgataa ttttatatag cttccatctt
     9301 gttcatttga attttttatc gtaaacgtac aaattggaac ggttgcttct tcaaaagctg
     9361 aatactccat ttgaatcaaa ctagaaatgt ttctttcatt aattaaaaat gagcgtaact
     9421 tttcatagct agaaatgaac atccaaacaa atggcgtcat aaaggaagca tatccattat
     9481 caatcagcat ttggctattc aatttaataa acacagaaaa tagatctttt ttgacatcag
     9541 aatagttatc atttacatat ttttttaatt tcttatccat tttattaaga taaggcggat
     9601 tagtgactac cgctacatat ttatcaacta gcatttcagc cacgtcaaga atgtgcaagt
     9661 atttttcttg tatttccttc atcccataaa gaatatccat attcgtaaat tcgttgaatt
     9721 ttgatatata ctctcttact tttcctaagt attctttagt tattgcctga ttatttttat
     9781 cgactaactt aatgattgat ccaagttctt tcccattttc aaattgatca aaaattaact
     9841 ctttgtattc attccagcgt tcactatact tatcattgtt caattcacta atacgctctt
     9901 gatactcgtc tgtcacttta ggagaatcaa taaactctct taaattcggc gatagacttt
     9961 ttttcaaaaa aagacgatcg tattgacgcc ctttcatcat aattgcaaaa taagacaatt
    10021 gatacgctct tttatcgatt tcaagaccat ataaattgta ttcaacgatt gcacgtgcag
    10081 catcacgctc agaatatcct aacgtaagat aaatttccat caatacatcg aaggcgtaaa
    10141 ctaaaatatg cccagacccc attgcgttat cgataatttt tagttcttct ggttttaaat
    10201 tacttacaga tgttgcttgt tcaaaagcat atttatctgg aattaagtat ttcaacttat
    10261 ttcttaattc actattggga taatgatcta accaatactt tcctaaacta ttgtcaacca
    10321 tataacgaac gacccaatct gtggtaaata gttgagttgc tgccggaata tcttgttttt
    10381 tgacaatccc tttataaata ttcaccactt gattatgtgg ctcactatta tagtactgat
    10441 acaaccaccc aataatctct acctgtccac cagcttgaac attgaaatca tcctctggaa
    10501 tacttaaaag atcagcgatt acaccatctt ttttaatata agaagctgta aacaaaagtt
    10561 ctgtataatc ttctacttct tcaaatagct caggtaagta atcattcaat tgattacaga
    10621 tttttataaa aatcaattga tacagcttgt ccattgcttc tgctgaatta tctgttttcc
    10681 aagttctgac ttgttgctgt tcattactag ataattcttc aaagatatcg gcatcataaa
    10741 tatctgtaat aatatctggc tcaattttcc cacttatttc actagataat acacgatacg
    10801 tgtcaggtaa ataatcattt acctccataa accgaatggc aatcaatcgg ttaaaccacg
    10861 tataagcaac ctcttcgatt aactcttgaa aagcttctga ataagaagtg acagaagcac
    10921 gttgttttag ttctgcaatc aatttgtttc tttgcgctat ttcttttcct tgaattctta
    10981 ttcctgattt ttcgatttct tgtaaattgc tatcaatttt cttcacatct tcaatttttg
    11041 attcatcaat tcctaaacgt tttgctttag tcattacttc atcaattaat tttctacggg
    11101 cattgaccgc gaaattcttt atcgctttct tatccatttt tctttccctt ctatatgtaa
    11161 cctttgaaaa gacaggcacc tttttatgcc tgtcttttca ctagaattga atcgtgattt
    11221 ctgtattttc ttgaagttgt tccttcagtt tttgattaat ttcagctgtt aactcatcaa
    11281 tatctgctgc tgacttaata gttttaacat gaacgttaag tgttctcatt ggtactgttt
    11341 tctgttgaat agtaggtttt actatcgcta tttcttcagc ttttgctgca gagtctattt
    11401 catctttttc agtttcacga atcaagtttg ctgcttcaat ttgtttacgt gcttgttctt
    11461 cttgacgtct gcggttttct tcattatcga aacgttgatt aaagatcaaa tactgttctg
    11521 ctacgtttaa tgataagaaa acaagatcat ctatagtatt cgcttgttca atttttgctt
    11581 tcagttcttg aaattcttgg tcaactattg gtacaaattg atccttaaaa tcttttcctt
    11641 ctaataattt aaatgttgct tgtttttctt ccgtgaattt atttaatatt tcctgacttt
    11701 ccaactctaa cagttgatta tattgttcaa taaattcttg aatcaactca tttagtttca
    11761 tcatatctga ataaggatca gcctttttca aaatggtttt catctcatga atcaattgat
    11821 caatttcttg gctagaagtt tgacgccgac ttttttcgat taatctaatt ttatcttgtg
    11881 cattctccca gatttttttc tgagtactat tttgcttgtg gaaattttta attttatcat
    11941 atgtttcagc ccagtcgaat aattcatcgc gatatttttt gattgtataa aaaaagtctt
    12001 caacatctga cgtttgaaca attacttcaa gatatttttt tatttgctca tattcataag
    12061 cactagggta ggtataagtg ttttgtaaat caaaataaga gcgtaaattg tctaattgac
    12121 cttttaaata tttcttgaaa ttttccattg tatgttcatt gttattttca tcaacaatat
    12181 tacgactttg aaacagttct ctaccgattt cgtcaaccaa tttttttgct tttggcgaaa
    12241 tcaccgcgct tggttcaatt tttatctttt cactttctcg tcgtttttga ataatcatta
    12301 caatactatt tggatcatcc atccgtgaaa tttgtacatt gttataaagt aaatcaattt
    12361 taccagacat gaaaagttta gcgatgcacc attccacatc aagttctgtg aatccatacg
    12421 gttgttttgt ttcaaaacga tcacgaattg ttttcatcgt aatcgttagg tgatttctct
    12481 tttgatcatt gataaaattg aaaatttcct gaactgcttg ctcatttgat ggatcttcaa
    12541 taatttcatt tttgctttct ttcactaaat ataagatatc ttctttttct ttaggcgcat
    12601 caatttggtt taatttatgg tatacttctt cagccatttt ctgaagtgct gattggaatt
    12661 ttgctttaaa gtcggagcct gtcagttgaa tttctgcacc gttatagaaa aattttgcag
    12721 atttcaacag ttcatttaat tgatcgttga tacttgcact tagttcctgt aattcaccag
    12781 atttttccga acgaatcgcg tcaaagttct ttactacact cgaataagtc gttgttaaat
    12841 aaagattcat tctagcatac attgctatat catctttaaa ttctttatct gatggcaatt
    12901 ctacgaataa taagttacgg gaactcttaa gcgcaagttc tgcctcttga cctgctaaat
    12961 cagagtatgg tgaaataaaa ttaactgtca tttcagcatt tccagcttgc ttgtatggac
    13021 gttgatctaa cacttgtgca taagtaaacg tataccggtt tttaaattta tttccacctg
    13081 acatgcctaa tttttcatag gcaaaactat ttcggtctag aacgccgtca aaaattcgtt
    13141 tagatagttc tacgatcaaa tcactatctg taatattttg atttttaatc tcacgagtaa
    13201 tttcctgttc ttcatcagtt tggaaaatat aactatctaa gttcttccgc acaaagtttt
    13261 ccttaattaa aaggtccaat gcttcttcta ctcgtttttt agtttccagg cggtcttcgt
    13321 tgatatttct tatcatcaag ctcgtaatat tttcaacatt tggtttgatg gcttgatcaa
    13381 catatttaat catgaataaa attcgtaaga catttacgtt aaagttatct atatcagttg
    13441 gtctttgaat atgagaatta tttgtagctt gtgtaatcac tcggctaatt gctgtatcaa
    13501 tccaacgttc taacgagtaa taaaactgat ctaaagaaac caaactggtt gtatcatttt
    13561 ccttatttcg ttcagcggct tctttaaaca ttgctagtaa tgaacgttca ccagacgata
    13621 agtttgatcc ttgatagctg tgattacgaa tctcggttag aatttgcccc aataaattga
    13681 attgataggg aataaatgga taaacttctg cgtaattttc agcatcttgg aagagcttta
    13741 attcaggact atcaacaaag agtaatttat ttttcaattc tgtttgaaca gtttcaaatt
    13801 gtaaggctaa agaatcattg gcaaactctt ttttcttcaa aatacgttct ttaattactt
    13861 catcggcatt tgctgaagat aaactcacag ttgtggcaaa acgatccttg atcttagaaa
    13921 aatcattatc agaattgtta tcaaagtttg ttacgtgatc aatcgcttcc tgacttgtca
    13981 ctaataccca agccttacct tcacaaattc gacccaaatc ttccacaacg gtttgtaact
    14041 gcaaagtcaa ctcacgatta ccgccgatat attgtccgac ctcatcgact agaaaggcaa
    14101 tatgatggtc tgcttctttg gaatcaagat agcttttaac tagatttgca aagtcatcaa
    14161 tgcttattaa ataatttcct gatgcttttt ctaaaaaatt atcaatagcg gcttcactca
    14221 taaaatcgat tttaagtgcc gcttctttga tatcatcttg gatccaatca aactcatggc
    14281 gctcttcttg ccattttgcc ccattaatct cttcaaaagc agctttgaat gcatcaaact
    14341 tttcttcttt aattaaccga cgttcaaaat ctgctagatg tggaatcgtg cttaatcctt
    14401 gcatttcgtt aaataccttt aaaaaaacat tcacgattcc gtttttgtct tttttaccac
    14461 ctgcatcact ttttgaatca atattaaaca gaataacatc tgtatttatt tgtgagcacc
    14521 gttccatcgt tgccaaagtc agtggatctt tgattttccc atcttctttg aagtaatcaa
    14581 tggctttctt gtcatttatt tctcgatttg cgagcaagta agaaataatt tttaagaaat
    14641 gagatttacc tgaaccaaag aacccctgaa tccaaacacc cattttatct gttggtgtat
    14701 tgatactccg ttggtaggca tcaaaaattg ttttaaagtg tttttgtagt tctttcgtaa
    14761 ccacgtattc ttctaattct tgttgaacat tttcatcctt tgcttgaccg accttaataa
    14821 ctccttgaat atttcggtca attggttttg caaaaatatc tttaattagc atttaatctc
    14881 ctaccttcca tcccaactaa attctaaatg ctcgataata attatcatct ttattggtat
    14941 taagaattgt caaacttaat tcatcatatt cccctggata catcatcaca actggtacgc
    15001 gatcaaatac ctgatgcatt ttatttaaaa tagagtgcgc tctaattaat ggatataatt
    15061 cgccaattcc tgtcaggaac acaactgatt tttctggtgt atgggcatcc acatattcaa
    15121 aaaatacatt ttcttcctca ggtaaatcca ttttcaataa acgaccaatg ctttccacta
    15181 atttttgtaa accacgtcgt tcttccatct taatcgcttt ttcagtgaaa ccgcgttttt
    15241 ctagatactc taacataatc acatagagat tataaacttt cacttcataa ttatcaccaa
    15301 cagatgttcc agatatatca ttaatataat ctcttacaat taactcgtct ttcggatcgt
    15361 agtcaaagat ataatagcct acttcattac ctaaaccacg attcaataaa aaatcttctt
    15421 tttgaattac ttcattcaac ttttccaaat gctcttgaat cgataatgac attataaaat
    15481 tcctcccagt gctagtacaa tatctttacg attcttgtca actaaccatt gaatcaatcg
    15541 tttatctaat aatatcggag tcattcgtcc atattctttt tcccctctga ctaatcctgt
    15601 ttcaatt
//
LOCUS       DACEPW010000053        15470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEPW010000053 DACEPW010000000
VERSION     DACEPW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.054"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(52..522)
                     /locus_tag="IW819_001304"
     CDS             complement(52..522)
                     /locus_tag="IW819_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1244418.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKITAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="IW819_001305"
     CDS             complement(681..1139)
                     /locus_tag="IW819_001305"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1244419.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="IW819_001306"
     CDS             complement(1310..2455)
                     /locus_tag="IW819_001306"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ1244420.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPHHELHGVAKQQMSGFSGMISFTLKNEETAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="IW819_001307"
     CDS             complement(2468..3385)
                     /locus_tag="IW819_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1244421.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="IW819_001308"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="IW819_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1244422.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="IW819_001309"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="IW819_001309"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1244423.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKERSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="IW819_001310"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="IW819_001310"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1244424.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /locus_tag="IW819_001311"
     CDS             6911..7792
                     /locus_tag="IW819_001311"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1244425.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGKEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /locus_tag="IW819_001312"
     CDS             7789..8505
                     /locus_tag="IW819_001312"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1244426.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="IW819_001313"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="IW819_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1244427.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAGTVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="IW819_001314"
     CDS             9222..10223
                     /locus_tag="IW819_001314"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1244428.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="IW819_001315"
     CDS             10220..11581
                     /locus_tag="IW819_001315"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1244429.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYSKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="IW819_001316"
     CDS             11541..12692
                     /locus_tag="IW819_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1244430.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFHYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSRSKEERVYNDEFIQSI"
     gene            12667..13836
                     /locus_tag="IW819_001317"
     CDS             12667..13836
                     /locus_tag="IW819_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1244431.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPKHVKQAGIAAIEEDFSHYTGMRGLEELREAACMFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14012..15391
                     /locus_tag="IW819_001318"
     CDS             14012..15391
                     /locus_tag="IW819_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1244432.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSVSDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         5062 a         2727 c         3287 g         4394 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtta ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcagt ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 ggggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct cgaatattca tattgcttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatatggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggattgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaattcttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 aaccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga gagaagaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgtttttg tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt actttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgatttccc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagaagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagcca atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatt gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tacagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 cggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggaaaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttgtcttatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat attggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagga actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atattcgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt taaacgacat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata aattatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga ttgaagactt
    11341 agctttgatc tctgtcgttg gcggaccaca taaagaattg atcggtttat ctggaaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa ccaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcggatt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 gggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaagggtata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatct ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 actataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagatcaaaa gaggagcgag
    12661 tatataatga tgaatttatc caatcgattt agtcccaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctaaacat gtcaaacaag ccggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atgtttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgataca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgca
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgaa tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaaaa ttaacgattg ttttttaaat ttagttcaaa
    13921 tgaacgcgtt tcttttgatt cataactaat gtaatgttaa gtttaaattg tttagaatga
    13981 ttttaaaaga atattcaagg aggtttcatc aatggaaaaa atcaaagttg tcattgtagg
    14041 tgcatctcat ggagggcatc aatcaatctt ggaactattg acacgatacg gtgaaaatgt
    14101 agatatcacg ctatttgaag caggagacta tatttccttc atgtcatgtg gtatggagct
    14161 gtatttagaa gacaaggtaa cagatgtaaa tgatgtacgt aatttcagac cagaaaactt
    14221 tccgcaatcc aatgttcata ttttaaataa tcacgaagta aaaacgatcg atgccgataa
    14281 aaaaacagta acggttacta gcgtatcaga taacgaaaca aaagaatatc cttatgacaa
    14341 attgattttg agttctggtg tcaaaccaaa caatctgcca gtaccaggag cagatctaga
    14401 aaatgtctac ttgatgcgtg gatacaattg ggcaaccaaa atcaaagaaa aattaaatga
    14461 tccggctatt aaaaaaatcg ctgtcgtagg tgcgggttac attgggatcg aagcagcaga
    14521 agcaagccga aaagctggga aagaagtcgt attgcttgat gtgatcgata gacctttagg
    14581 cacttattta gatgctgaga tgacagatat tttagaacaa cacctaaatg aaaacggtat
    14641 tgaagttttg acaaatgcaa aaatcgaagc ttttactggt aacggaaaag ttgaggcaat
    14701 caaaacaagt gaaaaagaaa tcccagcaga tctagtcatt caagctgccg gtgtgaaggc
    14761 aaatacagaa tggttaaaag ggatcgtcga cttagatgaa cgtggctgga tccaaacaaa
    14821 tgaatacttg caaacgaatc ttccagatgt gtatgcagta ggagatgcta cacttgctta
    14881 ttctatccca gcacgcaaaa aaatgccgat cgctttagcg actgttgcac gtagagaagc
    14941 acgttatgta gtaaaacatc tttttgaaga agtaccaaac aaaccgttcg gcggagtcgt
    15001 aggttcctct gcattaagtg tctttgacta tcattttgca gcaagcggat tgaatagctt
    15061 tacagcagca aaagcaggag tagatgtacg agtgtcttat tatgaagata cgttacgacc
    15121 aaaatatgta ccagaagaac aaggaaatcc aaaagtgtcc atccaattat tctcggatcc
    15181 aatgacccat caattgctag gaggagcagt attatctacc tatgatgtta caggtcaagg
    15241 aaatgtattg gcactagcga tccaaaacaa actgacttta gaagaactag cagaagctga
    15301 cttcttcttc cagccaggct ttgaccgaca atggagcatg ttgaatctag ccgctcaaca
    15361 agcgttagga gaagaaccat ttgtagaata agaaaacgaa atatttgaaa agaggttgtg
    15421 acagaagtgg tcagcttcaa gaaataagaa ggaattcacg aaaatcgttt
//
LOCUS       DACEPW010000054        15340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEPW010000054 DACEPW010000000
VERSION     DACEPW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.055"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..847)
                     /locus_tag="IW819_001319"
     CDS             complement(<1..847)
                     /locus_tag="IW819_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1244433.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFY"
     gene            967..1878
                     /locus_tag="IW819_001320"
     CDS             967..1878
                     /locus_tag="IW819_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1244434.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            1885..3531
                     /gene="mdcA"
                     /locus_tag="IW819_001321"
     CDS             1885..3531
                     /gene="mdcA"
                     /locus_tag="IW819_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ1244435.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            3633..3932
                     /locus_tag="IW819_001322"
     CDS             3633..3932
                     /locus_tag="IW819_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ1244436.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            3922..5598
                     /gene="mdcD"
                     /locus_tag="IW819_001323"
     CDS             3922..5598
                     /gene="mdcD"
                     /locus_tag="IW819_001323"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ1244437.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            5602..6195
                     /locus_tag="IW819_001324"
     CDS             5602..6195
                     /locus_tag="IW819_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ1244438.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            6219..7295
                     /locus_tag="IW819_001325"
     CDS             6219..7295
                     /locus_tag="IW819_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1244439.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            7320..8189
                     /gene="mdcB"
                     /locus_tag="IW819_001326"
     CDS             7320..8189
                     /gene="mdcB"
                     /locus_tag="IW819_001326"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ1244440.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            8300..9064
                     /locus_tag="IW819_001327"
     CDS             8300..9064
                     /locus_tag="IW819_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1244441.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            9553..11646
                     /locus_tag="IW819_001328"
     CDS             9553..11646
                     /locus_tag="IW819_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1244442.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            11671..11973
                     /locus_tag="IW819_001329"
     CDS             11671..11973
                     /locus_tag="IW819_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1244443.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            12000..13289
                     /locus_tag="IW819_001330"
     CDS             12000..13289
                     /locus_tag="IW819_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1244444.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            13308..13715
                     /locus_tag="IW819_001331"
     CDS             13308..13715
                     /locus_tag="IW819_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1244445.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            13738..14607
                     /locus_tag="IW819_001332"
     CDS             13738..14607
                     /locus_tag="IW819_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1244446.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            14609..15229
                     /locus_tag="IW819_001333"
     CDS             14609..15229
                     /locus_tag="IW819_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1244447.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
BASE COUNT         5039 a         2702 c         3304 g         4295 t
ORIGIN      
        1 aataaaattc ccgcatcgtt tttgcaatca ttttgatcaa aggatgatct tctgtgaagc
       61 tagaagataa ataaattgtt cgtgtcaagg gcaaatcttt gatttcccgc aagacgaaag
      121 gatcattttt atcgtaagac caagaaactt ttgggaaaaa gctaacagct aatccttgct
      181 tgacaataga acgtaatgtc gcaggatcat ctgtcgtcga actgatcgtc aattcgatac
      241 ttttcgcttc aagtgctgca tccagtgtat gacggaaggc gttgctcttt gacagcatca
      301 agatgggggt gcgttggata tcttctaatt gcagatgttc ttttttagca agaggatgtt
      361 tcttaggaat ggcgacataa acaggttctt tcatgatagg aatattggtc atgccaggct
      421 tcatctccga agtgatgtac agtacaggat cggtaatatc atctggtaaa tgatgttgca
      481 tgatcgttaa ttggatatca ggatgctttt ccataaaaat tcggaccaag ttcggaatag
      541 aaacagaggc tacctctaaa tacaatatga cggaagtaac gatcttctca ttgaccgaag
      601 acaataattt gattccttct tctaatgtgt ctaatgtctt gactgcatat ggataaaatt
      661 cttctgcata aacggtcgtc tcaatgtttc tgccgctatg gtaaaacaaa gggacaccta
      721 attcgttttc caaatgtttg atcgcacggc tcaaggcagg ttgagaaata aataattctt
      781 tggctgcttg ggtaacattt tttgtttcga atactttttt aaaatactgc agatgaagca
      841 atttcataat tatccatccc atccatacct ttttgttatg gttttttctt tttttggcat
      901 tttttattat agcagaaatt gactaagata gcagtggaaa aagtaagtgc tgaggaggcg
      961 aggctcatga gtacaggttt cttatttcct ggacaaggca gtcagaagtt agggatgtta
     1021 gcggatatcc ctgaaatgta catggaacgt ttagaaaatg tgacggggta tcggttagtt
     1081 gataaagaag aaaactatca agatacggtt tttatccagc tagcattact gacaaaagca
     1141 gtattttatt tagatgaaat ggaaaaaaac ggtattcagc ctgatattgc agcaggacat
     1201 tcgattggtg ctttttgtgc agctgttgct tgtcgatctt tgagatttga gaatgcagcg
     1261 gaactagtct atcaccgcgc agtcttaatg aaagaagctt atccaagcgg ttatgcaatg
     1321 ggcgtagtag taggtgtgac gagaagcgaa gctgaggaaa ttgtagcaaa aacatttgat
     1381 cctgaccatc cagtttatct gtcaaatgaa aattgtccga tgcaacatac gttatctggg
     1441 agtatcgaag gtttatccgt tacgttggca gaagccaaac ggcaccaagc gcaaacagcg
     1501 aagctgctga aagtccctgt gccatctcac tgtctcttga tgaatgatac ggtcaagaaa
     1561 ttcaagccgt atatgcagtc tgtccatcta tctgatgcta gatgtttgta tataaaaaat
     1621 acggatggaa gagccactca ggacgcagaa gagattcgta aagatttgtt ggaaaaccta
     1681 gctttccctg tgcagtggaa ccaaatgatg gacttggcaa aagaattagg aatggatctg
     1741 acgattgaat ttccccccgg caatactttg acaaagctga ttcatacaaa atttggtgta
     1801 gataaaggga tccgtacgat caatctagac caacatggta ttgatgacgc cacgtttcta
     1861 tatcaaaaat ggagatgact aattatggaa aaaccaaacc gcaactgggc ttctaaacgc
     1921 gaaaaaaaag cgcagcgatt ggaaaaaatc agcaacttgc tcaatggcaa gtatgtagaa
     1981 actgaaaaaa tcgtagaagt aatggaaaaa ctgattgctc ccggcgacaa agttgtatta
     2041 gaaggcgaca atcaaaaaca agcaagtttt ctttcagaaa gtcttgaaaa agtagatgca
     2101 gaaaaagtga atgatctgca tttgatcatg tctagtatct cacgaccaga acatttgaat
     2161 atttttgaaa aaggaatcgc aagcaagatc gatttctctt atgctggtgc acaaagcttg
     2221 cgtgtcgccc aaatgattga agacggcaca atgaaattag gtgatatcca tacttatttg
     2281 gaactttatg gacgcttatt catcgatttg attccaaatg ttgttttagt agctgcagat
     2341 aaagcagata aagacggcaa cttgtacaca ggtttcaata cggaagaaac accaacaatc
     2401 atcgaagctg cagctttcaa agatggtatc gtgatcgttc aagtcaacga agttgtcgat
     2461 tctttaccac gtgtagatat ccctggtgac tgggtagacg ttgtcgtgaa agcagataaa
     2521 ccttatcaat tagaagcttt attcacacga gatcctcaaa acatcacaga attgcaaatt
     2581 ctaatggcaa tgatggcaat caaaggaatc tacgcagaac acggcgtaca atctttgaat
     2641 cacggtattg gattcaatac agcagctatc gaattgttac tgccaactta tggtgaatca
     2701 ctagggttaa aagggaaaat cgcgaagaac tgggtattga acccacatcc aacgatgatt
     2761 ccagccatcg aatcaggttg ggttgaaagc atccatagtt ttggtggcga agttggtatg
     2821 gaaaaatata ttgctgctcg cccagatgtc ttttttgtcg gtaaagacgg cacgatgcgc
     2881 agtaaccgaa cattatcaca agctgcagga caatatgcca tcgatatgtt tatcggttca
     2941 accttgcaat tagattacga aggaaactct tctaccgtaa caaaaggacg tttatctggt
     3001 tttggcggtg cgcctaacat gggacataat cctggcggtc gtcgtcattc tacaccagca
     3061 tggcaatcac ttcgcgacaa tgatgatcca ttagggaaag gccaaaaatt agttgttcaa
     3121 atggttgaaa catatggttc aaataaaaaa ccagtctttg tcgaagaatt agatgcacta
     3181 gctgttcaaa aacaagcagg cttagccaat gcaccagtga tgatttatag cgaagatact
     3241 acacatatcg tgacagaaga aggaattgct tatttgtaca aagcaaaaac aaaagaagaa
     3301 cgacaagcag caattgcagc aattggcggt gttacacctc ttggaatgac tagcacgaaa
     3361 gaatcactag atagcttgcg tgcagccggc atcgtgaaat taccagaaga tctaggcatc
     3421 aaacgtagtg aagcgaaacg ttcattatta gctgcacaaa ccatcgatga tttgatcgaa
     3481 tggtcagatg gcctgtatga accaccaatg aaattcagaa cttggtcata aaattggcga
     3541 gaaaaacctt ttgtcgccat ttacccagat ggaagaagaa agacgaataa gcagaacgtg
     3601 tatgctctgt tttacaaaaa ggggagagat ccatggaaca attagcttat aaatatccaa
     3661 cgaaacagcc gatcaataag aaaatccatg taggagtggt cggttcagga gatttggaaa
     3721 tcttgatgtt tccaacagaa aaaacagaat ctactgtaaa aatttgtaca ggaagcgatg
     3781 gcttcggcga agtatggaac aatgtattaa cgagattttt tgatcgttat ccaatttcag
     3841 cagatatcgt gatcaatgac ttcggtgcta caccaggagt tgtgtaccta cgattgacgc
     3901 aagcaatgga ggaactttca gatgaaaaat agttttgtag aattaaacgc cagagaacga
     3961 gtgaacgctc tacttgacaa tggacgtgaa ttgattgatc cattcgatca aatcatcgcg
     4021 ccaagtttac cagcacaagg aatcgtccct gaaagtgatg acgggatcgt tgtcagccgt
     4081 ggcacgatca atggcaaaca agcagtagta atcgctatgg aaggaaaatt ccaaggtggc
     4141 ggtatcggtg aagtttccgg tgcaaaaatc gttgctgcac ttgaacatgt gttagaagaa
     4201 aataaaaaag gaaatgagat ctttccagtc attgtactag atacaggtgg tgttcgtttg
     4261 caagaagcaa actacggact gttatctatt tcagaaatcg gaaatgtaat cattgctcta
     4321 aaacaatatg taccagtcat cggtttgatt ccaggacgtg taggatcatt tggcggtatg
     4381 tcgattactt cagcattaat gagttattta atcgcaacaa aaaaagcgag agtcggtttg
     4441 aatggaccag aagttattga acaagaagca ggcgttcgag aatttgattc tagtgacaaa
     4501 gacttgattt ggaatacatt aggatctcgt caacgggtag aatcaaaaat cattgatgaa
     4561 ttagtgactg atagtgtaga agcaatcaaa gctgctgtca tcgcagcaat ggacgaacga
     4621 aaagacacgc atcgcagtga aagctcagca ttttatttat cattacttga ttctttagat
     4681 ctttcaaaac caatggccat tgacgattac aatgaagcaa ttgctaacca tcaagcaaaa
     4741 tcaattgact taccagaagt agcagaagga aatgaaccag ctgcagcaag tgttggttac
     4801 gattggtttg cggcactaac tggcatgaaa aatccaactt cttctattag tactgtgtat
     4861 tatggtaaat caagtaaatt catagaagaa gccatcgtga catcaatcgt acctgatcct
     4921 aaaaacccga tgtaccgtgt acgaaacggt gaagttggtt tagttgaagg tttccgcatg
     4981 ggacaaattt tggatcacgt ttacgaagaa gataaagaca aaaaagtgaa acggccaatc
     5041 atcgttgtga tcgatgtacc aagccaagca tacggctata acgaagaatt gatcggtatc
     5101 catgtggctt tggctaatag tgctgctgct tatgcaaaac tccgtcaagc tggacatcca
     5161 gttattggat tgattgcagg aaatgctatt tcaggtgcat ttttagcaca tggattacaa
     5221 tcaagccgct tgattgcttt aaacaataaa gaaattaccg tccaagcgat gtctaaagaa
     5281 agtgctgcac gtgtgacaaa acgttcggtt gctgaaatcg aagcagctgc tgaaaaagtt
     5341 ccagctatcg cttacgatat caataattat acaaaactag gagcgatcta tcatttccta
     5401 gaagatatca ccgaccaagc tgcttcagaa caaaatgtca gcaaagtcat cgaagcaatc
     5461 aatgaagcca ttcaagatgt ccgcaataca aatgacacga tgctgacaaa tcgatacaca
     5521 aacgaaacag ccaaaacatt cggacgtgtc gaaacaaata aagtccgtgc caaaatggac
     5581 gaagaatggg atgcataaaa aatgattcaa gcacatgata ttgtcctttt tgataaacat
     5641 gaaatagaag ggttcacctt gcctgaatgg gtcgttctaa ctgaaaagat gttcggaaca
     5701 gttcgtcgtg cgtcgacgga tcacgcagaa atactaccga ttggtttacg tggacctgcc
     5761 cgcaatcaga gatggtcaac agaaatctca aaacatgccg tcaaacaagt cattcatccg
     5821 tgggacatca tcgaacaaga atcgtataga caagagaaaa tcatggatta tcctgtttat
     5881 cagcaatttc atgctgctag aaacatatta tcaggatgta aatggggaat cggcggcagc
     5941 ttaggatttg aattatcgac tgggattccg gcagtaaaag aaaccagtga ttttgatttg
     6001 ctgctgtacg caaattcacc gattgaattg ccaattcagg taattcaatc gcatcctgct
     6061 ttttttgagc aattcgatac ccaagtagtg actcggcaag gcggtttttc actaaaagag
     6121 tatttgagag agccagagaa aaaaattcta ttgaaaacag tcgaaggacc taaattaacc
     6181 aaagaacttt ggtaataaag aaaaagagag agaaagagat gtttgaaaac aaaaaaatca
     6241 aaatcaacgt aagtgacttc cagaaagtat cagctgtaac ggctgttatg atgcaaacga
     6301 tattgagctt tgctttggca aacagccaca gcgcaacaaa tacagctttt cttggtacct
     6361 tttacgtcat cgttaaatac acagcaccaa tgtttatttt tgcaattgtt tataatatgg
     6421 taaaaacaag tcagcatttg tcttattggg aatttttgaa agaaaagttt tttgaactag
     6481 tcgttccgta tcttctatgg acgacggctt atctttggct atttccagca gtccaacaac
     6541 aaactccgta tacggatact gcaagctttt tactgaaata tgtcacaggt gatggagcgc
     6601 ctcatttatg gtataccgtt atgatgctgc agatccaatt gttcatgccg ttttttgttt
     6661 ggctcggtta caaagtattt gctaataaaa aatatgtatg gccagtattg attggttcaa
     6721 cgattttata tgtcgcatgg tatctgttct atcaaacaca agtattgact gggccttatc
     6781 atgacagctg gtacttatta gatcggtttg ttgctagttt catgatttat ggggtagccg
     6841 catttgttta tcacgaaaaa gtctatcaat atttggacag agtacgttat cttttcttac
     6901 ctgttgcctt agttatcgcg ttcttttctg tccgttcatt gctcgcacat ccaggagact
     6961 tgagctttgc gaatgcacct tacttgaata cgatccaaag tttatacagt ttagtcatca
     7021 tctttgctgt cttcataggc gcttccaaaa tgattgtaaa cgattcgcca aaattgccat
     7081 tgttcaaatg gttgtctgtt tatgcttatc gtacgtattt agccaatgta tttgttttcc
     7141 aagtactgtt attattgttt aaagatagtt ggctgcagtt accttcagga gtcatgattc
     7201 ttgtagctta tctaatgacg gcaagttgtg catttgcttt aagctggtta cttcatatca
     7261 tttgggtagc tatcaaaaaa ggtttcagca agtaatagta ggaagaagga gtgagtgtca
     7321 tgataaatga cgaacgaaaa aaagcagtga ctcagcaact cgctgcgttg gtagaggaag
     7381 cattgatcgc agaagtaaca ttaagtccaa aacctggatt agtggatgca ctggatgcgg
     7441 gtgcacattc cgatatggac tatgctttat ttcttaagtc tgctaagacg ctaactcctt
     7501 tttttgaaga aatggctcaa atagcatggc atcatgctat tgatcaggag ttaagagaaa
     7561 gaattgctga gattgggcga aaagcggaaa aggcaatgct gattgctaca aatggcatca
     7621 atacccataa aggagcgatt tgggcaatgg ggctattgat cagtgtcatt gcacaaaaaa
     7681 tcagtatgga acagcctttt tctttatcga aaatacttga tgatgtcagc aagcttgctg
     7741 cttttcctga tacaaaatat cgtcataaaa aaaccactca tggtcatagg gtgaagcaaa
     7801 aatatggcgt aaatggtgca tatgaagaag cgatattagg gtatccgcat atcaggcttg
     7861 caatccaagc agctaaggag ctagataatg attcatatgt acagaaacaa atgcatatgt
     7921 tgttagtttt gatgagttcg ctagatgaca catgcgtatt gcatcgcagt gatcagaaaa
     7981 cgttagtgga aatgcaaaaa cttgctcatg ctgctagtca aagtgcttta ccaaatcgtc
     8041 catttacagc actgttgcac ttttgtcagg aaaaccaaat ctctcctgga ggaagcgcag
     8101 atctcttgtc tgcaagcttg ttcatgctga agatagagga attatggact gccgacacat
     8161 acatttgtcc gatgttactc aatgactaaa taagacaaaa ttgctaaaaa aacagtttat
     8221 gcatctggtt attgactctt ccttttagca aggctatcat aaaaatagtt ttttgtaata
     8281 aaagataacg aggaagacaa tgaaaaaaat caattattta gcaatcattt tgttagtggg
     8341 cctatgtttg tttgctctgc cgacccaagg attcgctgtc gcgggtggtc atggtggagg
     8401 tacatctagc ggtcgaagtt cgggcagagg ttcatttggt ggtcgttcat atgggggcag
     8461 ttattttggc agtcgttctt acggcagaga ttcttatggg tattatggtc caggtacagg
     8521 tccttctagt tatgagcaaa cgggatttct tgcattagct gttttgtcag taggaggggt
     8581 ttctgctttt tctatcgtca agaataaaag acaaaagaaa aggctgaaag cggagatatt
     8641 gtcccgcatg tcaggaacgg ctaaagaaaa gaaagcacag tttctggaaa tccaaaagat
     8701 attcatgaag atccaatccg cttgggaaga agaaactcct gaaaaagcca gaagctgtta
     8761 tacagacaag ctgtttgccg aacatcagaa agttctgcaa aaaaacaaag cagacggtgt
     8821 aagaaatcat actaaaaaaa taactattca ggaagtagga aattatcgac agatccacga
     8881 aaatagtttt gccatccgaa ttgatttcag atgctgtgat tattcagtcg atcgaaaaga
     8941 tgggcgattg atctcaggaa gtaagacaca acgacagtat ttttctcaga tatggtattt
     9001 tgatttttct gagaaagaaa aaagatggca agccgatttt attcagccga ttatgctgga
     9061 ttgacaggaa atatttatga acggagttgt gacaaaagcc ttgtcgcagc tcctttttct
     9121 gaataaacag tgtgccagag atagcctatc ggcaataagg cacgattttg taaaatatgg
     9181 gaagcgattt ttcaaaatcg cttcttatcg ctcaaggctg aacatctctg tcacaacctc
     9241 tccactaaat ggtgtttaaa agcttgcatc tgcgataata agaacaacca aacgaaaaat
     9301 aggaagcact attttagttt agttgttctt attacttgat gcaggacagc tttttcacaa
     9361 cctctttttt gtatagatgg gcaaaatttt tgtaataata ggcgtatgtc tttttcgttg
     9421 aggagatgaa aaagcatatg gcttattttt tgtttgcttt gcttactttc tttccacttc
     9481 aagtggcaga aatatctact ttttattatg taagcggaac catatacttg aaatcagaag
     9541 gagatcattt ctatgaaaac aagaacaatt gaaatacttc acagaatcat gacagcccaa
     9601 tatccagtaa aagcaaaaca tctagcaagt gagttcaatg tcagtcagcg gacgatccgt
     9661 caggaagttc ttgaagcgaa tatctggttg agcagtaaaa agcttccgga gatccgtaca
     9721 attcgcaaca aagttttttt gttgaagctg gaaaaggaaa agcaagctcg attagaaact
     9781 gaaataaaag atgtgaaaga cggctttttg aatcggcgcg aacgtatttt tgatctagtt
     9841 ttagccgttg cttacagccg aatcccgatc catctaaaca aaaaagaaga agaatttcaa
     9901 atctctaaaa gtacattaga tgaagatatg agacggttac gagcaacact ggtcagttat
     9961 gggatagaga tcgtcagtta tggaaagcaa ggactggttt ataaaggttc agaacgatct
    10021 atccgtacga tgatctatga ttttatcaat cagaatctgg gaatgaccga cttctttatg
    10081 aatcaagatg cacaattaac accggctcaa aaaatctttc tccattattt tcctttggtg
    10141 gaaatcaaaa aaatacagga gatctattcc aataagttgt tgaaacaaga agatgatatt
    10201 tataaaaaac aaatcctgct ttttacattg atctggatcc agcgaatcag gaaacatgaa
    10261 tttatctctg ccattaattg gaaaaataca gaaataaaag acagtagctt ttctgcattt
    10321 attgaccaca tgatcgagga atttcaattg gacaatgtcc cgcaagtaga aagaaattat
    10381 cttcgtttca ctattgagac gttcaatgct agagatatct ccaattcaat cgagtgggta
    10441 caagctcaat tactaacgat ccaattgatc caatttgtag agaacgagac acacgtacct
    10501 ttccatctaa aggaagaagc tttattcgaa aatctctata agcacatggc tgcactgatt
    10561 gtaagaatca agaatcatat ccaagtcatg aatccattga aagaaaacat ccgaacaaat
    10621 tatttcccaa tctaccgcgc agtcactcat tttgttccta cgatcgaaga agtaattggt
    10681 ggcaagatac tggaggacga gatagctttt cttgtcatcc acttttcgac gatcgccagt
    10741 gtaatcaagc gagacttgaa ttatgtttat aaatcagtcg ttgtctgtaa ccatgggatg
    10801 gctactggaa atctgttagc agagaatctg aaagaaaagt ttcctcagat cgagattact
    10861 gcagttttaa gttcaaaaga aatcgaatta gtagataaat tggatgtcaa tctggttttc
    10921 agcacctttc aattgaatta tcgaaataag ccgcttttag ttgtcgatcc gattttaact
    10981 gagacgaata tcttggttat agaagacttt ttgaaaagaa atgaagaact acagcggtta
    11041 gagcctaaca gtcatgaatc gacggagtta ttcactcaaa taatagatgt catgaaagaa
    11101 agtggtggcc gagtagatcg aatcatctat ggcaagttag aaaaattatt cgaaacaaac
    11161 aatttagaaa tcaacaaaag ggagatccag cctatgttaa aagatatttt gacggacagc
    11221 aatatcatga tcaaagaaaa agccactact tgggaggaat cgattgaact agctgctaga
    11281 ccattggtta aagaaaacat tgtcgaagag cgctatgtcg aagcgatgat tcgtgcagtg
    11341 aatgattacg gaccgtatat cgtgattggc aaacacatgg cactagccca tgcccgacca
    11401 gaagatgggg tcaataaatt aggagtcagc gtagctacga ttgagcaacc aatcgatttc
    11461 ggtaatcctg aaatggatcc agtaaagatt attttttgtt tggcagcagt agactcctac
    11521 tcgcatctga acatcatgaa agaactaatt gaattgatca acaacgaaga gaagttggat
    11581 cgattgattg aatgcacaaa tatcgaagat ttcaaacaac tgcttttttc agtagttgag
    11641 aaataataaa tgatacgggg gagaacaaag atgaacaaat tgaacatttt attcgtatgc
    11701 ggtgcaggtt taggaagtag ttttgcagca cagatgtctg ctgaggatgt tttgaacaaa
    11761 ttaggagtag aagccaaact ggaccattgt gatatttctt ccgcagtttc catgaaacca
    11821 gatgtgatca tcacagcaga aaattttcgt tcgcagttcg aaaagttcgc agttgatcct
    11881 aaaacggcga tgatctattt gaaaaatatt gtttcaaaaa atgaaattga agaaaaaatc
    11941 accccagttc tgcaagataa gggagttatc taaatacaaa aagaaaagag ggtaaaaaca
    12001 tgggcgttat caattttatc atcgaaaata ttttgactca ggcatcgatc accattgcat
    12061 tgattgctat gttgggtcta ctactgcaga agaaatcagc aggacaagtc atctctggca
    12121 cgttaaagac actcctagga tttcaagtgt tgagtgccgg ttcgagcatc atcgttggaa
    12181 gtttaactta tttcgggaaa atctttactg aaggatttca tatgcaaggg atcatccctt
    12241 ctattgaatc tatcaatggt caagcaatga atgatttggg tttaggtaga gatattgcac
    12301 tgacgttctt agccattttt gtattcaata ttattttggc tcgtttcaca aaatggaagt
    12361 acattttttt aactggtcaa gcaattttat ggatggcaac gatgacgaca gtctttggct
    12421 attttgccgg tctgcgaggg atcgtattga tcttggtcgg tggttttatc ggagcttgtt
    12481 tcgccattgc tatgccggca gtagcgcaac cgatcattcg taaaatcact ggatcaaatg
    12541 atattgcttt aggtcatttt tgtacgatcg ggtacttatt tgaagccggt gttgctaagc
    12601 tttttggtga gaaaggcgaa aataaaaagt cgatcgaaga tatcaagttg ccgacacatt
    12661 tcgaatttct gcaagatact tatttgtctg tcatggtcgt gatggtcccg ctatatatca
    12721 tcactgtttt atttgcaggc gaaccattcg cttctgaatt atcaggagat caaaactata
    12781 tcatgtttgc ctttttacaa gccatccaat tcgtagtggg tgtgtatgta ttgctagctg
    12841 gtgtacgtct actgttaggt gaaatcgtgc cggcatttcg agggatagct atgaaattag
    12901 ttcctgatgc gatccctgcc ttagattgtc cagttttctt tccttatagc ccaaatgctg
    12961 tcattttagg tttcattacg acaacgattg gtacgattat tgccatgttt actttgccca
    13021 tgttcggctt agcgatgatt ttacctggta tgctaaccaa tttctttgcc ggagggacag
    13081 cagggatatt tggcaacgca gttggcggac gccgaggagc gatcattggc ggtattgctc
    13141 atggtttctt tattacatta ttgcccgctt tgctagtaac gatctttaat tcgatgggat
    13201 tcatcaatgc tacagcaacc gatgtagata cagtagccgc agcactctta tatgcctgga
    13261 tcttaagccc agttttgaaa gcattttaat tgaaagtagg gaaaatgatg tttggatttt
    13321 ttaagaaaaa agaaaaagaa ccgaaaaaag ttctggcaaa agaagaaaaa gccctatcgc
    13381 cagaagcgac cgaaaagatt caatcagaga ttacacaatt gaaacaagag atttccacta
    13441 cccaagataa gcataaatta gcgaaacttt atgaacaagt tggacttaaa ttttctgaat
    13501 tgtatgtgaa cgatcaagcc atccaatatt tagaaaagag tttggaaaat aaacagacga
    13561 tcggagatgg gtataaaaaa ctgatgagcc tctataacca gaagcgtgca gatgctgctc
    13621 gtgctggtga tgatcaaggg atcgattact acatgggcaa aatggatgag atgcgacaaa
    13681 ttgcaaaaca agtaacgatc aaaggaaaca aataaaaaga taaggtggca ataaaagatg
    13741 tatacaacat tgaaagaagt aacaaaaaca gcagaagagt tgaattttac agttggagct
    13801 tttaatacgc ataacctaga aatgcttcct gctatgctag gcgcagcaaa agaaatgggt
    13861 tcgccgatca ttattcaaac aagtgtcgat accgctaaat atatcggcat gcatgtgtta
    13921 gtcaatgtaa tgacagctat tgcagaagaa gaaatggttg atgcagtctt gcacttagac
    13981 cacgcccgag actttgatga tatcaaagaa gcaatcgaca gcggatatac ttctgtcatg
    14041 tatgatgggt cccatctgcc tttcaaagaa aatatcctga agacaaaagc agtagtagaa
    14101 tatgcccatg cgcacggtgt ttccgtagaa ggagaacttg gaacaatcgg cggtacagaa
    14161 gaaggaatcc atgtcgctga aaatgacaag gtatatacaa gaccggaaga cgcagtgaaa
    14221 tttgtagaag ctacaggcgt ggatgcatta gctattgcta tcggtacgaa tcatggacaa
    14281 ttcaaatcaa aaacagaagt aaacattccc ttgctgaaag aaatcgatgc agtagttgat
    14341 gtccccctag ttatccatgg aggaacaggt gtgaaagaag aagattatcc tgaattgatc
    14401 aataacggga tccgcaaatt caatgttgga acggagcttt tagtcaactg gacgaaagta
    14461 gctaaagaaa cctttagtga aacagaaatc aacaagtctc ttcgccataa tgtgattcca
    14521 gcaaatgaag cagtaaaaga aattgtcaag cataagattg gactgtttat gaacttggaa
    14581 agtccaatta acttaggaaa taaatgagat gaaaaagtat ttcattcttc tggctggtag
    14641 tcctggtaca ggaaaaacat atcttatgaa taaactacgg gaacgatttc cagatatgta
    14701 tgccatcacg ctcgatgaaa tcaaggaata ctatgcagaa tccatcgggt ttaacaattt
    14761 ggaagaacgg gcggaacaag aaaggacgaa agtttatcct ttctattaca aagcacttga
    14821 tttgtatatg gaagcaggaa agaaagtgat tgtgtccgag tatcctttta gtgataagca
    14881 aaaagcacga ctccaagaat tagcagatag atacggatat caggtgatca cgattcgcct
    14941 aacagcagac tttgaagtgt tatgtcaacg tcggtacaaa cgtgatcgtg aatcagatcg
    15001 tcatttgagc tatatcatgg atcattatca ctatggtgac gtattggaag atcgaacgaa
    15061 agggaccaat catattacga aagaagcatt caaaaagatc atcgccgacc gaaaatatga
    15121 caaattccag ttaggaacac tatatgaatt tgatgtaaca gactatactc aagtggatta
    15181 cactccacta ttggataaat tagaaaaaga aatcaatgac aataagtaac aaagaaatgg
    15241 cttaatatcg aaaaattggc ttttgaacgc tgtttaccga gaggtcgtga gcctgtcgtt
    15301 cagctccgag tcacaaggag caatttcaaa aaaacagctt
//
LOCUS       DACEPW010000055        15167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEPW010000055 DACEPW010000000
VERSION     DACEPW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.056"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..712
                     /locus_tag="IW819_001334"
     CDS             <1..712
                     /locus_tag="IW819_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244448.1"
                     /translation="LFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIY
                     YIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDY
                     ANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKG
                     QVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLY
                     RFTFSKQKQPRREVQHSH"
     gene            complement(857..1597)
                     /gene="truA"
                     /locus_tag="IW819_001335"
     CDS             complement(857..1597)
                     /gene="truA"
                     /locus_tag="IW819_001335"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1244449.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1788..2423)
                     /locus_tag="IW819_001336"
     CDS             complement(1788..2423)
                     /locus_tag="IW819_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1244450.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2570..3811)
                     /gene="sstT"
                     /locus_tag="IW819_001337"
     CDS             complement(2570..3811)
                     /gene="sstT"
                     /locus_tag="IW819_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ1244451.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4009..5007
                     /locus_tag="IW819_001338"
     CDS             4009..5007
                     /locus_tag="IW819_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244452.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5020..7038
                     /locus_tag="IW819_001339"
     CDS             5020..7038
                     /locus_tag="IW819_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ1244453.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNIIDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7098..7814)
                     /locus_tag="IW819_001340"
     CDS             complement(7098..7814)
                     /locus_tag="IW819_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1244454.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8041..8583)
                     /locus_tag="IW819_001341"
     CDS             complement(8041..8583)
                     /locus_tag="IW819_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1244455.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8588..8815)
                     /locus_tag="IW819_001342"
     CDS             complement(8588..8815)
                     /locus_tag="IW819_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ1244456.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8894..10774)
                     /locus_tag="IW819_001343"
     CDS             complement(8894..10774)
                     /locus_tag="IW819_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1244457.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10963..11577)
                     /locus_tag="IW819_001344"
     CDS             complement(10963..11577)
                     /locus_tag="IW819_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1244458.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11719..12279
                     /locus_tag="IW819_001345"
     CDS             11719..12279
                     /locus_tag="IW819_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1244459.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(12584..12898)
                     /locus_tag="IW819_001346"
                     /pseudo
     CDS             complement(12584..12898)
                     /locus_tag="IW819_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(13152..14787)
                     /locus_tag="IW819_001347"
                     /pseudo
     CDS             complement(13152..14787)
                     /locus_tag="IW819_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4818 a         2943 c         2389 g         5017 t
ORIGIN      
        1 tcttttttgt tcaaccaatt tatttccttg gcaacttttt aaccatacac cagttaagag
       61 tctacaattt gtctggcggt taaatggttt ttcttcttta tttatagcct atacgatgag
      121 tatagtgatc tattatattt tttctactaa aaataattca tggaaaataa tggtctttac
      181 gtttcttgca ctaatattgc atctctcagg agtaagcaat tctatccatg ctaacaaaga
      241 ttcgttaaca cttatagcac ctgaagacgc tgttgctata gctgaaaatt acaatcatac
      301 tgattatgca aacaaagagt caatctatca tcctgatatg attaataatg atcaatattt
      361 actaggtaac caggaaataa atccaacaac tcattttatg agtaacaaac ttacaattga
      421 attagataat tcgaataata aaaatactgt cttgacgaca cctatttatc gttataaagg
      481 gcaagtggct tctattaatg gcaaactagt acaaacaaaa ttatctaagt ttggcacaac
      541 tgaattaact attcctccag gtataaataa agtcgttatt acttatcagt acacaaaact
      601 agcaattgct tcaagatatt tatctatcgt tactttgatt ttatttttac tttatcgatt
      661 tactttttct aaacaaaaac aaccgagaag agaagtacaa cattctcact agatttttat
      721 ttaagaaaga ctttttagct gtacacataa acaggctaaa aagtcttttt ttatatttct
      781 ttaatttgct tcatacatta tttaacatcg atatttatac tgtgaaacat gcgtttcatc
      841 cattatcaaa gtatttttat cgataataaa cttcatccaa aaacagacct tgtgcaggga
      901 cggtttctcc tgcatgttgc cgcactttgc tttcaaatat ttcatcgatt gtttctatcg
      961 gcatggcacc tgtgccgatt tctagtaatg tccccatgat aatccgcacc attttatata
     1021 agaatccgtc tccgacaaaa gtaaaatgaa gcatttctcc ctttcgttcg atagataatt
     1081 catcaatcgt acgcactgtg gatttttttg attttttcaa tgcagaaaaa ccaatgaaat
     1141 catgtgtacc cactaatttt tcgcaagctg cattcagctt gtccatatcc aactcttgag
     1201 ggaaataaaa gctgtggttt cgctcaaatg ctgaaggaat cacgttgttc caaacataat
     1261 agctgtattt tttgcccacc gcattatatc ttgaatggaa gcgctcaggc atttcttcga
     1321 ttcttttgat gacaatatcc cgcggaaggt aacgattcat aaagtcgatc atttctgcca
     1381 gtccaatttc tgtgtttgtt ttaaaatttg ctacttgtcc tcgcgcatgg gtgccagcat
     1441 ctgttcttcc tgatccaata atttctatct cttcatttgt catctgatgc aatacttttt
     1501 cgatttttcc ttgaatggtt ttatcgtcat ctccaagtct ttgccatcct gaatatcttt
     1561 ttccgtcata ttcgattgtc aacttgatgt ttctcatatt ttagtggctc ctctgtcttc
     1621 catttggtct tctctctacc tttttaagat tgtagcacaa aatatagctc cttgcttctc
     1681 taagacagaa gctatacaag aaaactgtgg aaatatatat ttcagaaaaa caaaaaacag
     1741 ccaccggatg aagtatccgc tgactgttca atttgacggc tcaagtttta ctgtttgaga
     1801 tgcaagatac tcaaccaggc aatgggcaca taattaaaga aaaaactgat tctttggcaa
     1861 aaacctattt tattcagtaa tggccaagaa tttatagaag gaattcttgc cagtccataa
     1921 atcgccgcaa aaagcaaact gatcacagac agtccaaggt ataagtggct tacgtccatt
     1981 tgcccatttt ttcgatagat caagaaaaga agaactggaa acaataaaaa accactgtaa
     2041 cctaaacctg atccgatatt atggacccat gtcgagacat tccacgaaga ttcatgggta
     2101 ttgatactga acaaacctgt aaagatacaa tcacctatac catagaacgc gatggctgct
     2161 gtaactaggg tagctaacac acgagatgta ctaacaaatg cagcatacaa tgcaggcaat
     2221 gacaggatga aaaagatccc tgaaaccaca gaccaaataa gaaacgcttt tcttacagga
     2281 ctggaaacct ctccaaatac actgatcacg ctcttcattt gatctaattc aggataatac
     2341 aatcctaata cgtacggagt caaaaaatca ctcagcacgc ctaataataa aaagaaaaaa
     2401 ccaaatttac gtaaaaaatc catgatacgc ctccaataac aattacctat gcttttaatt
     2461 gttattataa acgactttat cttgatttaa cagaaacctc tatttacacg aacatctata
     2521 taactcatta aaaactcgaa tcattttaaa aatactaaat atctaacgtt caacgttcac
     2581 ttaatccatc taaacaatct ttcaatttga ttttttcccc acttctttta cgcgcgccta
     2641 gctctcctat tgcagtaaac aacagatcac ttgacgaatt taaggcagtt tccattgaat
     2701 cctggataac accgataata aatccaatac cgacgatttg catcgcgaca tcattagaga
     2761 taccaaacaa actacatgct aatggaatca ataacagtga accgcccgca acacctgaag
     2821 ctccacaagc agctaaagca gacaaaatac ataaaaggac tttcaaaaca aaaggtgctt
     2881 caatccctaa agaatgtaca gcagtcaacg tcatcaagga aatggtaatc gcggcaccgc
     2941 ccatattgat cgtagcacct aacgggatcg atatggaata agactctttt gtcaatttca
     3001 atctttcagc caacatcatg tttatcggga tatttgcggc agaacttcgg gtaaagaaag
     3061 cgggaagtgc gctttctttc aaacaaaaga aaatcaatgg atagggattt tctttaataa
     3121 acaaaaacgt gaggaatggg tacacaacta atgcgacaaa cagcatcgta ccgatcaaaa
     3181 caagtaaaag ctgcagatac tctgccaatc cagcgattcc agtcgtagca attgattgat
     3241 aaaccaatcc taaaatccct atcggagcaa atgcaataat cacttgtacg acttgtgaca
     3301 acgctataga tatctgatcc accaactctt ttgtcgattc tgaacgtgct cgaaaagcta
     3361 gtcccaacaa tacagcccag aataagacgc caaggtaatt cgcatcaata agagattgaa
     3421 caggattttg tgtgacattc gtcaacatag tagataaaac actttccaaa ctttgcggtg
     3481 ctttttcaga tactgtttct tgtaaagtaa taggaacagt aaaaattgaa cttacaataa
     3541 ctgctacaag tgcagaaagc aatgttccca ctaaatataa gatcaaaata ggcttcacga
     3601 atgtcttatt cccaatttta tgcttggcaa ttgacgccat cgtcaagaaa aaaaccagaa
     3661 taggtgcgat tgccttcaag caactgacaa acaacgttcc caatattgaa ataaatgtcc
     3721 atgtggggaa aaagatacca aacaatattc cgactaaaat accaacaaca atacgttgaa
     3781 ctaacgacat atccaaaaac ttctctaaca aatgaccacc ctttcacaat tttaacaatc
     3841 attgtatcat tttatgcaaa gaaactaaag gtataatttt ctaaaaattc taacaataca
     3901 aacaaccatt tcttgctaat tttgtacttt ttctgtctac acgttgtttt ttgagatatt
     3961 ctaagttagg atgttattaa tgggcaactt atcaggaaac gttttcttat gtttttgtcc
     4021 aatttttttg tgaggtgttt attcttgaaa aaaataaaga aggagagtta tggcttcctg
     4081 acaatctttg tcgcttgtat aatgatggtt ttacagcgta tattgatttc agcagtcact
     4141 attcctacat atgcaacaaa ccctggtcga ttctacatct atacattttt acaaacttcc
     4201 gttgttattg gtgcaaattt gatcccactt tacatgggtt accaatatca aaaaatcgcc
     4261 cgtttaaaag ttttacatta tttaagtcgt tttgccttta tattcctaat agccacacta
     4321 gtttgcaata tcttttttta cgtacaagct ggcagcttga atattcgtga ttattggctt
     4381 atcctaacac ctatcagtca gaattacttt acttatgctg tttcttgtac tttgcttttt
     4441 tgtagtattc cttatacagt tggattttta aataaattat cgaaaacaac aattaaatat
     4501 ctactattat ttttaacaat tggattagtt ggctgctcta ctctatttaa taaagacccg
     4561 tttgcattac agaatggaca gagtatttta tggatttttt atttattttt aatgggttat
     4621 ggtttgaaag aatggaactg gaaagaaaag gtccgctata agcttcctca agtatttatc
     4681 agcttgattg ttttattcgg tttgattatt ttgatgacac aaatttcact catcatcaga
     4741 ggaaatacag atacagcgct gaggttttct aaaccatatt ctctcttcgc aatgtattat
     4801 actttctctt cgtttttatt tttagaaatg ctatctgata aattaggtat aaaaggaaga
     4861 ggaaattttc ttgctgtatt tttgattgtc actcaagtta tatgtaattt ccctttagtt
     4921 tcttatcgcg tatctacatt tatgaaacgt gaatttccaa actcagggaa ggcatggctg
     4981 ataaatatcg ggcagttttt tatttgatat ttattagcag tgtccctcct agttatcttt
     5041 ttattttata tacaaaaaat ccgtttcatt catcgtttca tcgaatactt cgcttttgat
     5101 tcgattgaac aattaactca gcgttggaaa aaaagaattc attggctttt tgttcataaa
     5161 agttattttt tagttgcagc atttttttac tgctttacct tcgtgcagat tttcgtttta
     5221 gaaccaaaag aaggttggaa agaaactatc caggttgcct taaaaatatt tactcagcga
     5281 caagcaccca tgatcctgac aatcatcatt attatgggat ttttcttctt actattgttg
     5341 atttccaatc gtttttggta cgccttgact gctaccttga tcattaactt actgctcact
     5401 atttcgacag taatcaaaat ggaaatgcgg gaagagcctg tctttccttc agatttaaaa
     5461 atgctgaccg gattgtcaga actattatcg atggttagcc cagtcttatt aatcgtgggc
     5521 ggattgatcc ttctcctatt attgattact agtattattg tacaacgtag actacaaaaa
     5581 cagtattcct tgaagattca ttggaaacgt cgatttatca gtatcgctgt attattagga
     5641 tgtttttcgg gtgtcttttt cattaatcac aaaaattcgc cttctttctt gctgtttaat
     5701 ttatttaaag taaacaagac tttctttaac cagcaaggcg ccgtaaaaga taacggacca
     5761 gtcattcaat tcctaaacaa tatcgacatt aaaatcatgg aagaacctgc tggctattcg
     5821 aaagaaaaaa ttaatggaat aatgaaaaag tacaacacaa aggcagatga aatcaaccaa
     5881 acaagaagtg actggttaaa caatcagacg atcgttctca atttaagtga aagcttcagt
     5941 gatcctgcac gtgtccctaa tctgacagta cctacagacc ctattcctta catcaaaagc
     6001 attataacag aaggaacagg aggattgatg ttatccgtcg gttatggtgg cggaacagct
     6061 aacatggaat gggaaggact gacaggcttg agtatcagta acctgtctgc ttcgttagtt
     6121 acaccttata cccagctagt ggagcgtcag aaaataagtc caaatattat cgatctattc
     6181 gatgaaaaaa tagccatcca tccttttaca gctgctcttt atcgcagaaa acaagtgttt
     6241 gataaatttg gttttgacag gttctattat ttagacagtc cggataaatt aacttatacg
     6301 gataaaatag aacaaagccc acgcatatcg gatgattcag catacaaaga aaccttgaaa
     6361 gctttacaaa gcaatacgga aacgtcacaa ttcatccaat tatccactat gcaaaaccat
     6421 atgccttatg atgattacta tagtgaattg aactacactg ctgaaggaac tgctgtcatt
     6481 cctaatagaa gaaaagaatt acaaacttat atgcaaggcc ttcactatac cgatacagca
     6541 gtaaaaaaat tcatccaaga aatcgacaaa atcgataaac cgattacctt tgttttttat
     6601 ggtgaccatt tacccgcaat ctatagtggg aatgattcga aaaaatatgg attagaacag
     6661 caccagacag attatttcat ttataacaac aagtatagtc gtgaacaagc agctacgtta
     6721 aataaagaag ttgttgctcc taataacttt ggtgctatgg ctcttgagca tgcgaatatc
     6781 aaagtaactc cttactatgc gttgttgaca gaagtatcta atcatctacc tgcattaaca
     6841 attgatccta cagaaagctt gtctaatcgt tttaatggaa aacaggtctt tgttacagag
     6901 gatagtcaaa ttatccaaga aaaaaattta actgagaaac aaaaagaaat tctagaagat
     6961 tatcgtctga tacaatatga tcttgttgca ggtaaccagt acagtgcaac atgggcgaca
     7021 caaaaaataa aagactaaca aataaactga gcaaggggtt gtgataaaag acatgtcaca
     7081 accccttgtt tatctcatta atttgcttgt ccataaacga tttgattaaa gccatttttc
     7141 aattcattat tcgctgtctc gttgaagtaa agataattat cgggattccc ctgattttgt
     7201 tgtgtcacca atacttttgg ctgattttgc tgaaacccga atagatagac gtgctttttc
     7261 agatatggtt ctgtctctgc atcggaatag acggctgtga tttgatagtc tgcttgacct
     7321 gtcccacttt ctgaccattc tgcagaaaca ggcactccac caatagccat cttccactcc
     7381 ccgttcaaaa tagcttgagg gactttaaga ccatataagt ccacactcat ttgattcgta
     7441 taacttttgt attgttgtcc cattgtttgg ccccatttgg tcataaatgc ctctaattgt
     7501 gcggatttgt ctgcattcca cagtgtttgt tcaacttgtt cgctgctttc tgaagaggaa
     7561 gatgaagcct ctacagaaga acttgaagta gatgtttgag ttgtttcagt cgttttggaa
     7621 gactctgacg aagtagaggt atctgctgtt tctgtacttg ctgcagttga cttgctctct
     7681 gtgctagttg ttgtagtagt tgcaactgtt gctttcgctg tttcttttgt tgattctttt
     7741 ttcgattcat tcccgttggt acaaccagct aacaaaaaag ctagaccaat tgttactaaa
     7801 atcctttttt tcaattaaaa aactcctttc gattactccc acttctttat tctatcaaat
     7861 aagcaatgtg attttattga tttttcgtat tttctcatca atctttcaaa tcttctaaaa
     7921 actgagaagc acccgctgat gttgtctgta atacatcagc gggtgcttct tttattttaa
     7981 tcttcgtgat tttccaattt acctcacgct ttatatttgt atttgaagaa acatagactt
     8041 ttagtcatca tcgtcttcct catccaaatc ttgtcggaca gtttttcctt ggagtctttg
     8101 tacttggtag atgatttctt tattgaaact taaaagttca acaagcaatt gttgcaagcc
     8161 atatttttct tctttttcag ctaatttgat tgcccgtgtg ataaagacat tttggaaatc
     8221 aaaatctttt gcaaagtttt ccaacattgt tgaggctggt aaatatttgt tttgaccatg
     8281 ttcgtaaatt tttccataac gcatgacttc ttctaaagta gacggaacga tttcattttc
     8341 aatcactaac tcatgagaga ttttattgaa ccatctcgct tcttgatcag catattctga
     8401 aaaaagggta cttaattctt gcgaagatgt acccaaaaca tacaattgtg cttggatcaa
     8461 tttcatttct tctaatttaa aatttgctaa tacatgcccc accatggcgc cagcagttgg
     8521 tttatgatgg tcgatatctg cttgttttgt ttcttgaaca aatttttctt ccactatatt
     8581 cattgattta cgcctctttt acttttgctg attggacttc ataacctaat ttttccacaa
     8641 ctgcagctaa ttgttcaggg tggacttcat ctggatcaaa gtttgctttg attttgctcg
     8701 cattaaacaa aactttcgta ttgcttaccc caggctgttg agaaaccgca tgttcgattt
     8761 tttgcataca gcttggacaa gctagtgtat ctaattgcat aataattttt ttcatcgtag
     8821 ttcttccttc ctttttcttg atagtcttat tctagattac agcttccata aaaaaattga
     8881 ttcttatcaa gttttacgat ttttcttctt tgctttcgtg tccaaaatac aataaacgca
     8941 tcgcgttaaa gataacgatc aagatactag cttcgtgaac gaacatccca ctagccatgt
     9001 aaataaaccc ggcaaataag ccgttgagta aaaataagac gacaccaatg gcaatcacta
     9061 cattttcacg ggtatttaaa acagttttct tagccaagcg gtaggcatga accaacgatt
     9121 caaaattaga ttgcatcaag acaacgtcac tcgtttcgat agcaacatcc gttcctgatc
     9181 ccatagcaat cccgatatca gccgcagcca aagagggaga atcattgatt ccatctccaa
     9241 taaaagcaac gcgcaatcct tgttcttggt attttttcac aaattgaacc ttttcgtctg
     9301 gtaataattc agcatggact tcatcaatgc ctaacatatc agctacataa tcagcagtca
     9361 tctgattatc acctgtcagc atcactaact gttttgctcc tgcttgtttc aacttagcta
     9421 attgttcagc gacttctggt cgaatcacat ctgacacacc tatgatttga gtgatttttt
     9481 tgtcgattgc tacaataatg accgagctgc ctgtttgttg taattgatgt aaatcttgta
     9541 cttgctttgg tgtgagagaa agtttgtgag cggcaatcaa tttggtgttg cccgcacaaa
     9601 ctgtatgacc atcaatctca gctgtgattc cttgtccctt gattgtctga ttatctttga
     9661 ctgctaattg ttgaaaatca atgtctttct cctgagcata atggatcacg gcgcgcccca
     9721 gaggatgatc ggataacgtt tctaatcgag ccactaaagc taagctgtcc ccttcatcct
     9781 ctgtatagtt cttgatagtg gatacagcag tagttccttt ggtcaatgta ccagttttat
     9841 caaagacaaa agtatcgatt ttggaaaaag tatccattgc ttcaccgcct ttgactaaga
     9901 tgccccgttt cgctccatta ccgattcctg ctacattcga aacaggcgca ccaatgacta
     9961 aagcgccagg acaaccaaga accaaaacag tgattgctaa acgaaaatca cgactaatca
    10021 gaccaacaac caacgcaata agtaaaacgg ttggagtata atactgagaa aatcgatcaa
    10081 tgaatttttc cgcatgggat ttggtatctt gtgcttcttc tactaattcg ataattttgg
    10141 caaaggtcgt atcatctccg accttagttg cttcaatcgt cacgtagcca tctgctaaaa
    10201 tcgttccaga gaagacttca tctccactga ttttgacgac ttgtcgtgat tcaccagtca
    10261 ctgcggcttc atccgcatac cctttcccgt caatgactcg tccgtccaca ggaatagctc
    10321 caccagtttt tactaagacg atatcgcctt cttccacgtc atcgatttcc acttcttcag
    10381 tcgttccatc ttcttggaca acactagcag tagatggggc catctcggtc aaagccttga
    10441 ttgactcccg cgttttggtc aatgtttttt gttccagata gctaccaaac aaaaacaaga
    10501 atgtaacgat agctgactcc ttgtattctc cgatcataaa ggcaccaagc acagcaatag
    10561 taacgagtaa atcaattgag ataactttga ctttcactgc ctgatacgca tggatcataa
    10621 taggtatgac cgcaataata gaagctatgg ctagagcaat gttatagaac aagacaaaac
    10681 cagtaaattt tccgataaaa ccaaaaataa tcaacacagc tgataaggcg gtgatatgat
    10741 ttcgatgctc ttggatccac ttttgtaatt tcatctttct ccctcttttc ctttcattct
    10801 tacgagacta agtatagata cttatccatc ataaaaaatt gaccactatc aagtttagtc
    10861 aaaaaggcgg ttttcttttg gtagaataca ctacaaaaaa tagttagcaa gaagactcac
    10921 taaaagtgat cttgccaact attgtcgttt ctatcgaaat tttcattgaa tgattttcct
    10981 gtacagcata catcggagat ctatttcttt atctaagatt tttatcttgc taattttttc
    11041 ctttagtctt tcaaatccac gtcgttcata gaattgatac gtacagccac tatctgtaaa
    11101 aaggaatatt tcttttcctt tctctctttt ttcaaattct gttagcaact tggtaccgat
    11161 gccttttgtt ttcaaatcag gatttgcagc taaaaaaatg atctctccat ctggcgaatg
    11221 cttctttaaa tatgctgcaa gcatttgttc atttgctgtc tcgtaagctc ctttactttc
    11281 tttagcaaat atattttgta aaaaatcgaa tatcttaaca tatatttgct gtccaaaaga
    11341 acgatgtttc tttttttctc cttttatctc cgccagcaaa acacctgcca actcgtcctc
    11401 tgcatacaaa gcaagaattt gagttgctcg attcaactcc atatacaaaa aatatcttcc
    11461 gtaaagattc agcagaagtt cattttccat atatctctca aagttcattc cttcaacagc
    11521 aaaatagata gcttttttgt aatctttctt ttctaagggt ttaaaagtta gtttcatcat
    11581 ttcgcctcct caacttcaat gattgtgtta tacttacatt ataatagaca cagtgtctac
    11641 tatctataat caatcatcta taagtttgtc gattaagcga cagctttgct ttgatatgtc
    11701 ggataaggag ataggtaaat gaaaaagaca gatagacgcg tcaaaaaaac agaaaaagct
    11761 ttggctgaaa cactatccac attattagta aataaaaaga ttcaagcaat cactatacgc
    11821 gaactaacag aaacggcaga tgtacatcgt tcgacttttt atacacatta taaggatatc
    11881 tatgatctat atgatcaatt agaaagcaac ttttttcggg atctaaatga aattctctct
    11941 tatgatccag cccatagcta tgaggaacta tacactcgct tgattgacta tgtttacgca
    12001 aatacttttt tatatcgctt atttatcctc aaccatggtg atatttcgtt tcagaagaaa
    12061 gttaaacaag ttattgaaga aaactatctg aacatatggc tatatgaaga caagaaaacg
    12121 attatcacag aagaaatgag attctttact acgtaccacg tccaaggttg tctatccgtt
    12181 ctcaatcggt gggtagaaag caattacaag tactccaaaa aagaattatt agaactctta
    12241 cagaaactga atgatcacat agagaaaatc atgccttgaa taaaaacata ctaagcttag
    12301 ttaagaagaa ccgtaacctt ctcgtataac aactgaatct tatttagcca aattgtttac
    12361 tgaagatttt ttgagtaaaa tttctataat tcctacttat tgaaaagtag tttgtagtgc
    12421 tgcttctccc tctctaagtg atatatcttg ttgtaccaat caaattcacc tcatcattaa
    12481 taaattactt ttaggctaat ttattacaac ttataggttg aagctgtaac caatttatag
    12541 tcatattata tttttatcat ccagtttatt gaattcttct cgtttacctc aattcatttt
    12601 caaggggaat cagctcatca tctaacattt tacgccatgc tcgcttcatt tcttgtgggt
    12661 atttaaatgg tttcgcttct ggatgtttgg ctttatatcc ttcgtaatcc ttactgttga
    12721 taattgcttt catattagga attggatctt cacctggcat atattgcaga ttagaagcat
    12781 gtaatttttt ctcagaaaca aaccactcat tggaaaagac atgaatcaaa tcatcactta
    12841 cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc attgctatct agtgtacctt
    12901 catttctttg tgcttaaatt caaagataat tttctcaata aagatttttg attccagtaa
    12961 gattccttta ctccttttac aacatagcgt tgtcctttgt tttgaaacat cttcttcccc
    13021 tctttgccat taaaaatagc ccaattctct ataaagaaga ttaagcttca ttattataat
    13081 ataaagttta aatttggttt attacttact ttttcttatt ctctagtaag ctaatattga
    13141 aaaaaatgta atcagatcat tatttttata tgttgtaact tcctgataag aaaactaatt
    13201 aaaagaaaga ggtaactaca taaactaatt aaaaaagcta acttcatagt tggaggataa
    13261 atggaagaaa cttcaatgat tgtttctcct tgtgttcctt caatcgacac tagtcctatt
    13321 atcaatctct cttttttgtc catttacttt tgcgactgtt ccccaataat caaatacagg
    13381 aagcgttatt ttttgtcctt tagattcatg tactgtatag actattttcg aatccatcac
    13441 ttgataaggc atagtatgcc attccttgcc aattcttact tcatgattct gcaaatgttc
    13501 tttgtattgt aatgcttccg tgggtgcata gtcataaagt cctccgggaa ggagtgtcgt
    13561 gtaacgaata agattttctt gattcattac caagtgatct ctctgagtga caactttatg
    13621 ttcatagttt gcttttgttg cagcagatac agccaacagt aataaaacaa ttgccgttat
    13681 attaactact tttctttgct tagctgatgt gctcttcatg aaaacgaacg ataaaatcaa
    13741 cgagccaaac agtacttgaa agcctaagat tcgccaagga aattgaatca tttgaatggg
    13801 tgtattttgt aataaagacc atggaaaaaa tttggtggat aagagccaag agactaccgc
    13861 actaaggtaa atcacttttt ctttgcgatt aaatccttta aaaaagaaga gtggacagat
    13921 caaagaaata ataccaatga ttcctaccgt aaaggttgtc aggtcattat tcatcgcacc
    13981 aatcaaacta tccgagacat ttaatgcttg gtgttgtaag gtcgttttat ctggctcatt
    14041 aatcgattga accatgattt gtcggaacat cggataccaa aagtatgctg ttaaaagaat
    14101 cgtcattcct actgcctttg acaactccac aatcaaccgg atgctttctt tttttcttgt
    14161 ttcacttact acaaagccgc ctaagactag taagccaacg gctattacac tcataaaagt
    14221 ggaaagaaca tgtgtataaa tgagtaagct catactgact gctaatggaa ttgcacatgg
    14281 ctttttctca taaatcagtg cgtaagcata atacataact atcggtataa ccgtaagtgc
    14341 aatcgcttca gcaatagcag aacgatgata aatatcaatc gtacggtacc cataaaatgt
    14401 atataaacag ctaaatataa aagccgcatc gattcttttt aaaaatttta gtccataatg
    14461 aaacatcagc atgatagtac aaacattcaa tagccaaaca tataagatat aactaacgat
    14521 taaattatta cttatccaat aaaaaatgac tgccggaaaa aaggtcaagt agggataaaa
    14581 ataattcact ccgctcccat aatgattaaa cgtcgtaaaa ttgattggac cagaaaaaat
    14641 tgaagataac cctttgactc tagcaaaatg gaaggcaata tcccccgatg tataaatagt
    14701 atgaacattt ctaaaataat tgattgaata aagtccagca ataattgcta ggagaatgga
    14761 caatattatt tttttcttta cttgcattac ttaattatat actcctttca tggattgctt
    14821 tatctagtat aacttaaatt ttaaataaat cgttatttat ttttactctc acttaaaaaa
    14881 gcactaaaat tataagagag ggaggtaatg gtctgaacac caagaattgt tccaacatcg
    14941 aattgcttaa ctaatgagtt caaagcttaa ttatgtggct cttatattta ccgagcgtgg
    15001 attgcgatct ctttagcgaa acaaacatct ttcataaaaa aaacagccat atccgctttc
    15061 ttggatatga ctatttacta ctactttact atctttcatt cttcttgtct cacttatgat
    15121 aaggcgctgc gtatcatgag tgaatgatat taggaaaact tcataaa
//
LOCUS       DACEPW010000056        15099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEPW010000056 DACEPW010000000
VERSION     DACEPW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.057"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(315..398)
                     /locus_tag="IW819_001348"
     tRNA            complement(315..398)
                     /locus_tag="IW819_001348"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="IW819_001349"
     CDS             926..1426
                     /locus_tag="IW819_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1244460.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="IW819_001350"
     CDS             complement(1616..1960)
                     /locus_tag="IW819_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1244461.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2114..3034)
                     /locus_tag="IW819_001351"
     CDS             complement(2114..3034)
                     /locus_tag="IW819_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1244462.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3221..3712
                     /locus_tag="IW819_001352"
     CDS             3221..3712
                     /locus_tag="IW819_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1244463.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3736..6111)
                     /locus_tag="IW819_001353"
     CDS             complement(3736..6111)
                     /locus_tag="IW819_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1244464.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6437..7063
                     /locus_tag="IW819_001354"
     CDS             6437..7063
                     /locus_tag="IW819_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1244465.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7086..7790
                     /locus_tag="IW819_001355"
     CDS             7086..7790
                     /locus_tag="IW819_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1244466.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7982..8815
                     /locus_tag="IW819_001356"
     CDS             7982..8815
                     /locus_tag="IW819_001356"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1244467.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9012..10940
                     /locus_tag="IW819_001357"
     CDS             9012..10940
                     /locus_tag="IW819_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1244468.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11078..11533
                     /locus_tag="IW819_001358"
     CDS             11078..11533
                     /locus_tag="IW819_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1244469.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12003..12272
                     /locus_tag="IW819_001359"
     CDS             12003..12272
                     /locus_tag="IW819_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244470.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            12672..12926
                     /locus_tag="IW819_001360"
     CDS             12672..12926
                     /locus_tag="IW819_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1244471.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13015..14061)
                     /gene="holA"
                     /locus_tag="IW819_001361"
     CDS             complement(13015..14061)
                     /gene="holA"
                     /locus_tag="IW819_001361"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1244472.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14099..14890)
                     /locus_tag="IW819_001362"
     CDS             complement(14099..14890)
                     /locus_tag="IW819_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1244473.1"
                     /translation="MDVGQGDSIFLQSPFRKETILIDTGGQLHFEREKWAQGMIRPPA
                     ERNIVPYLKGQGISVIDKLILTHDDTDHVGELPTLSQHFTIKKIYIGWGAGRNEPLQR
                     HLSEAQKNGTEIIEIKHGDRISGYYDLYVLTPFEKGEGRNEDSIGLWMEYNNRRFLFL
                     GDLNQAMEKQLLLIYPNLKADVVKLGHHGSRTSSNTDFLSHIEAKHGIISCGVNNRYG
                     HPHSEVLETLEENQIKTWRTDQQGMITYRWHPVFHPHGLVETMID"
BASE COUNT         4628 a         2853 c         2660 g         4958 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat
     2041 tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa
     2101 taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg
     2161 ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta
     2221 aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt
     2281 cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag
     2341 acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg
     2401 gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta
     2461 cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga
     2521 agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc
     2581 cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt
     2641 ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa
     2701 tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat
     2761 tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag
     2821 ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata
     2881 aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa
     2941 tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt
     3001 tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc
     3061 tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac
     3121 tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag
     3181 atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt
     3241 cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa
     3301 agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag
     3361 caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt
     3421 tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca
     3481 cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa
     3541 cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca
     3601 agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt
     3661 attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa
     3721 cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa
     3781 acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg
     3841 ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat
     3901 tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc
     3961 aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat
     4021 cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa
     4081 tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat
     4141 caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt
     4201 catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac
     4261 cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc
     4321 agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt
     4381 cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag
     4441 tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa
     4501 actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct
     4561 tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc
     4621 gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg
     4681 aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc
     4741 gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc
     4801 ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa
     4861 acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc
     4921 ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc
     4981 aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt
     5041 tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa
     5101 tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt
     5161 gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc
     5221 attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc
     5281 tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc
     5341 atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc
     5401 ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt
     5461 aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag
     5521 ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt
     5581 gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt
     5641 tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc
     5701 ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact
     5761 atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa
     5821 atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt
     5881 gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt
     5941 ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat
     6001 tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg
     6061 caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac
     6121 ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa
     6181 acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg
     6241 atgaatctaa tgtaacaagg acatattaaa atgctatgaa aacttacttt ccttctcttt
     6301 agaatatcca aatacttttc atatagtctg ataattcaga taagtaaaga gaaataagta
     6361 cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa gttttggcta
     6421 tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg aagaaaactg
     6481 ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag atgcggaacg
     6541 aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc ttacccatac
     6601 tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa tcccagttta
     6661 tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt ctggactcgg
     6721 tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa
     6781 aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc attctatagg
     6841 aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt ttagaggaag
     6901 tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata gtattcggac
     6961 ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg atgcaacgac
     7021 gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact taggaggaga
     7081 ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaacgatg ttcaatacaa
     7141 ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc
     7201 attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct agtgacagtg
     7261 gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga aaagaaattc
     7321 cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa ggtggatatg
     7381 acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat gatgaaatgt
     7441 ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta gataccgctg
     7501 ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt gaagatatcg
     7561 cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat ggtttgacga
     7621 tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg cttttaaatg
     7681 gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc atcaatgata
     7741 cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa tggggatatt
     7801 ttaattttta ccagagaatc ataaaaggca aagtctgaac gagggctttg tcttcttttt
     7861 tctcttcgtg tacttatctg ctttatttct caagtttttc ttaatattaa aaaacagatt
     7921 gggtttctca aaaaataacg gtatactata agatgtatct taattgggaa ggaaggaatt
     7981 tatgtttttt gcaaatatta tcaaagcagt gattctcggt attattgaag gaataacgga
     8041 atggctgccc atcagtagta ctggtcattt gatcctagca gatgaattca tcaaactgga
     8101 tgccagttca caatttatgt cgatgttcaa tgtcgttatc caattagggg cgatcttggc
     8161 tgtcgtcgtt ctttattttc acaagttaaa cccattttct ccaagtaaat caacggtaga
     8221 aaaaaaagat acgtggacac tttggtttaa agtgatcgtt gcttgcatac cagccatcat
     8281 cattggtttg cctttggatg attggttaga agcaaatttc cacaagttct taccagttgc
     8341 cattgttttg atcgtttacg gtattgcctt tattattgtc gaaaaacgca acaaaaacaa
     8401 aacacctcgt tggacttctc tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt
     8461 attccaagtc ttggcacttg ttcccggaac atcccgttct ggcgcaacga tcctaggatc
     8521 tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt tcatttttct tagggattcc
     8581 tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag ggaattcatt
     8641 caaaatggaa gaaactgttg ttcttcttgt aggaacattc gtatcttttg tggtttccgt
     8701 cttagctatc aaattcttac taagctattt gaagaaaaac gactttaccg cttttggctg
     8761 gtatcgtatt atccttggta tcattttgat tggttattgg tttattgcaa tgtagaaaaa
     8821 accagaacac ttctcttatt tgagaaatgt tctggttttt tattttcttt aataataatc
     8881 attataaata ataaaaacaa catcaagctt aatcaccaaa tgataaccat tatcgttctc
     8941 atttagctgt tattattatc aattacattt ttattatgct atccttgttt tgacaaggag
     9001 gtctaataac gatgactaat cagaaaaaag cttttgcagc aacaatcggc tgccttttat
     9061 ttatgatctt cggttttatc ttcgaccgat tctctcttac catcacccca cttttttata
     9121 tcagtgccat tatcatcggt ggatataaac aaacatcgga aggcttgaaa gaattgtgga
     9181 atgatcggac actgaatgtg gatttattga tggctttagc tgctattggt gcctgtttga
     9241 tcggtaatta ctttgaagga gcaatgttga cattcatctt ttgtttaagc ggtgcattag
     9301 aggaatacac aactaataaa agtcaaaaag aaattactgc tttgatgaat cttcaaccac
     9361 aaaaagccca attattaaaa gaaaatggac aaatgcaaga agtagatgtt tctcaactaa
     9421 aggtggataa cttggtattt gtcgctaaag gagcagctgt tccgatagat ggctttttag
     9481 aaagcacaca ggcaacgatc gatgagtctg cattaagcgg tgaatctgtg cctgtagaaa
     9541 aatatttagg cgatgagatg tatgcaggaa cgctgaatca aggaaatccc atcacgattc
     9601 gagtaacaaa aacgagttcc gaaactgtct ttgctaagat tatccaatta gtagaagaag
     9661 cgcagaatac gccaactcaa acagcttcat ttatcgaacg aatcgaaaat aattatgtaa
     9721 aattgatttt gcttgctgtt cctctaatga tccttctccc ccactttttc ctaggttgga
     9781 gttgggatga aagcttttat cgaggaatgg tattattagt tgtggcttct ccttgtgcgc
     9841 ttgtcgcatc tgctacacca gcttctctag ctgctctttc aaattctgca aaaaatggga
     9901 ttttaatcaa aggcggtatc catttagaaa agctttctga attaaaagcg attgcctttg
     9961 ataaaacagg aacgctcact aaaggaaaac cagttgtaac tgacagcttt ttctttgatg
    10021 aaaaggattt ggcacaacaa cttcttgttg ctatggaaca aaaaacgacg catccacttg
    10081 ctcatgcgat tttgcaatat ttcgattgca caatacctga agaaatccaa tacttaccaa
    10141 tagaagaaat tactggtttt ggattacaaa ctacctattt aggtgcgcaa tggaaagttg
    10201 gcaagcacgc atatgatcct gagacgatga taatcagcaa agagattgcg gaaatgatcg
    10261 aacgtttgga aaatcaaggc aaaaccgtga tttatctttc taaagatcag cagttaattg
    10321 ctgttttagg tctgctagat attccaaaag caaataccca acaagtaatt tcctacttta
    10381 aatcccaaaa catccataca agtatgataa ccggcgacca ttctggcaca gcaaaggcca
    10441 ttgctgaaca agtagggatt gaacattact atgcaagctg tactccggaa gaaaaaacac
    10501 aactagtcaa aaaagagaat gaactttacc aagtaaatgc gatggttggt gatggagtca
    10561 atgatgcgcc tgctttagca gccgcttctt taggtgtcgc tatggggcaa gggacagatg
    10621 ccgctatgga tatcgcagac atcgtcttaa tgaaaagcga cttaaataaa tttgtctaca
    10681 gtcatcggct atctttaaaa atgcggcgga tcatcaaaca aaatattatt ttttcgattc
    10741 ttgttattct cttgttgatt gcttctaact tcctgcaatt tttaaatcta cctcttggag
    10801 ttgtaggaca cgaaggaagc acaatattgg ttattctgaa tggcttgcgt ttactaagac
    10861 ctttacctga tctttctgat tcttcaaaaa aatgtaagtc ttgtccacta tacaaagcgc
    10921 ggctgtcttc tgtcaaataa gcaaagtgtt tttttatgat tttgtttatt ttatgaaaaa
    10981 aacagcttat gaataggtct tgaaggatga attcctactt ttgtaacttt ctaaaaacat
    11041 gttatgattg tttcgaagaa agtgaggaat atatttcatg gaaagttggc tttttttagg
    11101 aattatttta cttgtagcaa ttttagctaa aaatcaaagt ctgatgatag cggcagcttt
    11161 tgttttgatt atgaagctca taccagcctc ccgttccttt ttcccttggc ttcaaggcaa
    11221 aggaatcaat attggggtga cgatcatcac agcggctatt ctaatcccta tcgcaacagg
    11281 agaaatcggt ctacgtgatc tattagaagc ctttaaatcc cctattggct gggtagctgt
    11341 tttttgcggt gggctagtgg ctgtcttatc ttccaagggt gtaggattga ttgcttctga
    11401 tccacaaatc actgttgcac ttgttttcgg cacgatttta ggtgttgtct tcttaagagg
    11461 aatcgccgcc ggaccggtca ttgcttctgg tatcacttac tgtatcttac aagtatttgc
    11521 tttaggaaaa tagaacaaat tttgtctatt ttaggagatt cttaacaccc ccaatttact
    11581 gatccgtgat atactgacaa cgaggtgata caatgttcga aaaattaaag tatttcatag
    11641 gaacaatcgg ctggtttctt ctagcggtac ttttgattct gggcttagct tatctattat
    11701 ttcctgattt gatacaaaat gtgtttgata ctgctttttg attgacctat taaaacgata
    11761 taggggatgc tcctgcgatg caggggcttt ttttaatcaa tgttttgtaa taatttaggc
    11821 cgtgccaatg agacttccta tagaattcga tgctcttttt tctttttggc tcttctgctc
    11881 ttttttgtta tactagatta cagtggacaa aagaaagaat gatcgcatcc acttcaacga
    11941 atatcaaaat ggaagaatta gtgcctgttt tcaggttact ttgaaaagaa aggaagacgc
    12001 ttgtggaaac ttttgaaggt cttttaattg atacgtacgt tttacaagaa cagccaatga
    12061 tgctgactgg gatcaacttg cagcttgagg accgtgtgat caatggcctg cttaaagatc
    12121 gctatttggc aaaatatatc ttacgtttgc ccctaaataa atatgtgctg gaagtaagtg
    12181 gcaaatggaa ccgtcggaac cagttggtca taaaaaaaat gttgataaaa aatatcgatg
    12241 aatacattaa aataatcggt acaccagaat agcctttact gatcaaagga tactggttat
    12301 aaaataactg cagtttctta atagaaaaac aaagaacaac agggaatcgt atcccttttc
    12361 aaaaaaaact ttgcaaacgg ttctctttgt tatataataa cttagtacca aacattccca
    12421 ttcaaacttt gtttaaatag ataaataaaa atctagtttg gtttgacgct attgcctccc
    12481 acaatagcgt ctttttgatt tttttcagct tgtactatcc gtttttactg aaaaaaaatc
    12541 ctaataaaag actgttaagg aaaaaaactt gtcatgttca tattgcaaag cacgctgcct
    12601 ttaggtataa taatgaatgt tgaattatgc ggtaacacgc gtaagaactt aagttatcgg
    12661 aggtgaaact aatgccaaac attgaatctg caatcaaacg tgttcgtaca agcgaaaacg
    12721 ctaatgtgaa aaattcatct caaacaagcg ctatgcgtac tgcaatcaaa aaatttgaag
    12781 atgctgtagc ttcaggtgct gacaacgtag atgcgttata taaagaagct gttaaagcta
    12841 ttgatatggc tgaatcaaaa ggtctaatcc acaaaaacaa agctaaccgc gacaaatctc
    12901 gtttaagcaa aaaaatcgct aaataaaaga atcggcttcg gccgattttt ttatttgtca
    12961 aaaaaaggac aaaaacaaac gaccttcccg catgctttgc cctcgtcttt cttcttatcg
    13021 atttcttaac aactgccctg tgcgcaaaac gaatagttga aaacttaatt ctttttcgat
    13081 ttgtcccgtt ttgatttgat aatccatctc aactaattga tcaaacaact gtaccagcaa
    13141 atgttcatcg aattttttta cttcctgcat ggcaagtttt acacgatatg gatggatttt
    13201 caatgtttca gcaatatttg cctgctgata accaatcttt acaagaaatt ttgtctgtaa
    13261 aaataaacgt aattgagaaa gcaaaatcgc attaattttg attgtttctt ccccttgcat
    13321 aactaaatct tcgtataaac gcaaagcttg ttccgtcttc ccttttaaaa tatattgggt
    13381 catgtcaaaa atattatgtt ctaacgtttt aggaactaat tcttctactt ctcttaccgt
    13441 gatttgctgt tgattttgtc cataaaggac caatttctgt aactctctca tcattttgga
    13501 taaatctaag tcggtcaacc tcaagaataa atcaattgca cttcgttcca ttttgattga
    13561 ggaattctct aaggtattca tcagatattg acgtacttct ttttcattca actgttggac
    13621 attgatgata attgctgttt ttttcaattg tttcgttact tttttccgtt catccaactt
    13681 ttcatatggc gcaaagaata ctaatacagt cgattctaat ggttctttca aataagccgt
    13741 caataaatca gtattttgtt cgattccatt gtttactttt tcacctgtta aaaaataagg
    13801 attctctacg aatactaacc gttgatctcc gaaaaacggc aacgtctctg cttcagctac
    13861 tacctcatct aaacttgcat tttccatgtc aaaacttaaa aaatttaagt catcttcgcc
    13921 ctctaattgg atttttttga caatttctgt ccgaacttga tcttggagaa acttttctgt
    13981 tcctaaaatc aagtaacaag atgcaagttt ttctttacgt attttttgta actctgcttg
    14041 tatactcaaa ttactcacca ctttcaaaaa ttatcctacc atgagcagat gagaattact
    14101 agtctatcat tgtttccact agcccatgag gatgaaaaac tggatgccag cgataggtga
    14161 tcattccttg ttgatccgtt cgccatgttt ttatctgatt ttcttctagt gtctccaata
    14221 cttcgctgtg cgggtgacca taacgattat ttacgccgca ggatataatc ccatgttttg
    14281 cttcaatatg ggaaagaaaa tctgtgttac ttgacgtacg gctaccgtga tgacctaatt
    14341 tgactacatc ggctttcaga ttaggataaa tcaaaagcaa ttgtttttcc attgcttgat
    14401 tcaaatctcc taaaaataaa aagcgcctat tgttatattc catccataga ccaatcgaat
    14461 cttcatttct tccctctccc ttttcaaaag gagtcaatac ataaagatca taataaccgc
    14521 tgatcctgtc cccatgtttg atttcaatga tctcagtacc atttttttga gcttcagata
    14581 aatgcctttg taatggctca tttcgtccag cgccccaacc gatataaatt tttttgatag
    14641 taaaatgctg agataacgtg ggtaattctc ccacatgatc cgtatcgtcg tgtgtcaaga
    14701 tgagtttatc gattacactt atcccctgac cttttagata tggaacgata tttctttcag
    14761 caggaggacg tatcatacct tgcgcccact tttctctttc aaagtgtaac tgacctccag
    14821 tatcgatcaa aatcgtttct tttcgaaaag gactttgcaa aaagatactg tctccttgac
    14881 cgacatcgat aaaagcgatc gttgaagaaa agcgtaaaaa tggcaaagtc aaaaaaataa
    14941 aagaaaaaag accaacataa aacaagtgtt tcacaggttg ttctttcaac ctatccatac
    15001 aaatcagtaa tccgaaaata tacatcccag atagcacaag gggcaaacgt cctataatga
    15061 gagacgaccc tgaagaaaaa tcgattattc gctcaaata
//
LOCUS       DACEPW010000057        14892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEPW010000057 DACEPW010000000
VERSION     DACEPW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.058"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(13..831)
                     /gene="purR"
                     /locus_tag="IW819_001363"
     CDS             complement(13..831)
                     /gene="purR"
                     /locus_tag="IW819_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1244474.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(998..1822)
                     /locus_tag="IW819_001364"
     CDS             complement(998..1822)
                     /locus_tag="IW819_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1244475.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(1795..2475)
                     /locus_tag="IW819_001365"
     CDS             complement(1795..2475)
                     /locus_tag="IW819_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244476.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(2498..3436)
                     /locus_tag="IW819_001366"
     CDS             complement(2498..3436)
                     /locus_tag="IW819_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1244477.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(3681..4532)
                     /locus_tag="IW819_001367"
     CDS             complement(3681..4532)
                     /locus_tag="IW819_001367"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1244478.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(4686..7259)
                     /gene="mprF"
                     /locus_tag="IW819_001368"
     CDS             complement(4686..7259)
                     /gene="mprF"
                     /locus_tag="IW819_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1244479.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            7400..8206
                     /gene="proC"
                     /locus_tag="IW819_001369"
     CDS             7400..8206
                     /gene="proC"
                     /locus_tag="IW819_001369"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1244480.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(8285..9841)
                     /gene="rny"
                     /locus_tag="IW819_001370"
     CDS             complement(8285..9841)
                     /gene="rny"
                     /locus_tag="IW819_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1244481.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(10252..11301)
                     /gene="recA"
                     /locus_tag="IW819_001371"
     CDS             complement(10252..11301)
                     /gene="recA"
                     /locus_tag="IW819_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1244482.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(11410..12651)
                     /locus_tag="IW819_001372"
     CDS             complement(11410..12651)
                     /locus_tag="IW819_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1244483.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(12891..13469)
                     /gene="pgsA"
                     /locus_tag="IW819_001373"
     CDS             complement(12891..13469)
                     /gene="pgsA"
                     /locus_tag="IW819_001373"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1244484.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(13678..14562)
                     /locus_tag="IW819_001374"
     CDS             complement(13678..14562)
                     /locus_tag="IW819_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1244485.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         4270 a         3305 c         2590 g         4727 t
ORIGIN      
        1 tttcacaacc tcttattctg aaaaataatt tcctggcaca accgtaatat ttttcgtttg
       61 agtatctaca tctttgacat aaagcagtga cgtataatca tcgatggcgc gatgaccgtt
      121 gaattttgat tcggcaaata cagtgatgcc aacaagttct gattcgaatt cttcaatcaa
      181 gctcttcatt ccgttgactg ttccgccgcc tttcatgaaa tcatccacta caaggacacg
      241 tgaaccacgt ttcaaacttc tcttagacag ttccatcttt tcaattcttt cagaagatcc
      301 agacacatag ttgacactta ctgttgatcc ttctgttatt tttgagtccc ggcgtacaat
      361 aacgaatggc gcattcagat aataggacac agcttgggca ataggtacgc cttttgtggc
      421 tacagtcatt acagcatcga ttttttcacc aaggtattta gaagcaatga ttcttccaac
      481 ttgacgcagc agttcaggtt ctcctaacaa atcagagaga taaacatatc ctcctggaag
      541 cagacggtct tgttctgata gacgttctgc tagagattca atatattttt tcgcctcttc
      601 atatgaaatt tcagggatga acaatacacc acctgcagca cccgggattg tttccaaggt
      661 tccataccct cgttctttaa atgttttctt gatgattccc aaatcttcac tgatagaaga
      721 tttagcagat tcataacgtt cggcaaagct tgttaaggaa atcaattcat gcgggtgatt
      781 caataaatac tgtgtcatgt caactagacg ttcactgcga cgtactttca cctattccaa
      841 ctccttttaa gaaattttat cgttttacat tatataagtt tttcttcaaa aattcgagca
      901 ttttatgaaa aaataagaaa atagttcggt atttattaaa ataaaaagaa gcctataaaa
      961 aatttttcgt ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg
     1021 caaccaatat tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga
     1081 ttgttgctcc tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta
     1141 gaaatcctac acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac
     1201 cgattgctgc gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca
     1261 tcacagcaat agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta
     1321 gcccatccac gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata
     1381 aaaagaacaa tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg
     1441 taatggtcac gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc
     1501 tcaaattcat caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga
     1561 tttctgaata ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa
     1621 cgactagcat ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg
     1681 ctaaagagac atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca
     1741 cacctagcat cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga
     1801 attcatatga aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc
     1861 cgcatactgt ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac
     1921 atgcgcacta tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt
     1981 tgttggttca tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat
     2041 tcgctgcttt tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc
     2101 tacagcttct aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca
     2161 gcggcctctt ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc
     2221 attgaaagat gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt
     2281 tttctcagca atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac
     2341 caaggttgac ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac
     2401 atgataagag acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat
     2461 ttcgatataa cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag
     2521 ctttcagatt tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag
     2581 tcaagctttc taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct
     2641 cagctacctt cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca
     2701 caaattcttt tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg
     2761 aaatcgctac ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag
     2821 tgacaaatgt gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt
     2881 caagcttttt gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt
     2941 tttcgcctaa ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc
     3001 aaacatgtgg atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt
     3061 ctgcttcttg cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt
     3121 tgctatccac tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca
     3181 cgaaaacatc tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg
     3241 gctccgttcc agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg
     3301 taaaatcata catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt
     3361 ccttgttcgt atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca
     3421 aaagatattt tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat
     3481 taacgtgaac taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg
     3541 atttaagcat gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag
     3601 ccatttcact acaataaaaa actgaagaat accattttag gattcctcag tttttcatca
     3661 cctattttac ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa
     3721 ccttttaacc cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg
     3781 gttggtccgc ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg
     3841 atttgatgga tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt
     3901 ttgatcatct gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga
     3961 tgttggatgg cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt
     4021 ggtttgacca acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct
     4081 ttgccaccga caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc
     4141 aaaccgattt ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct
     4201 cttagagcag ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc
     4261 ttcttaacaa agatcttgac accggttttg atattgtatt tattttttag caataaagca
     4321 gcttgataaa cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg
     4381 attttgtctt ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc
     4441 accatttcca actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt
     4501 atcttggctg gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc
     4561 gcaagtattc tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt
     4621 ttgtcatcgt ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt
     4681 gattcttata attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt
     4741 cctatcagac tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca
     4801 aaataacgtc cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag
     4861 gaatagaaaa aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa
     4921 aaatctgttt ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc
     4981 attttctcgt atacaataaa cagattggct aacagatagt cagctaattc ctcagattct
     5041 ttaggtaaga cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg
     5101 atgaagccaa cgactttttg atctttacgg acaataccga caccgctaga taatacatat
     5161 gatttatcaa atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt
     5221 gaaacttcat ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg
     5281 tatccttctt tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct
     5341 gttgctagat caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga
     5401 agcatcacta cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct
     5461 gcttgtttca taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct
     5521 cctaaaacaa agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg
     5581 tcttcttggt aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg
     5641 cctccgtatg tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc
     5701 gtagtatctt cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa
     5761 ccaataagcc ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt
     5821 attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa
     5881 aagccgaaca agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga
     5941 taaaattctt ttctggataa ccacacagct aacagaatca agacatatac caatatcaac
     6001 tgccaagata acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta
     6061 ggcagaaatg cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag
     6121 ccaaccaata gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga
     6181 aatagccgac tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc
     6241 gcaaaataga cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga
     6301 atgttatcta aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc
     6361 gtcataaaag gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca
     6421 tcttgccgaa cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt
     6481 ccacctggga ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc
     6541 gaaagcatcg aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct
     6601 cccgtccatt gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg
     6661 aagaacaatg tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta
     6721 ccgccgaaaa gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca
     6781 tcaataaatg taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt
     6841 gatacagtgg ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga
     6901 cttgctccta ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca
     6961 gcaagaagaa actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc
     7021 gtcacccaat catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc
     7081 aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca
     7141 ttagcaatat ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt
     7201 tttaaaatca gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca
     7261 ttttcccatt cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt
     7321 aatttttcca ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa
     7381 tgctaaagga gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc
     7441 tattattgat ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag
     7501 tggacatttt gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag
     7561 taatcaggaa gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat
     7621 tgaaaaagta ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc
     7681 ttcaggaaaa tcattaatag atttagaaac agcgcttgaa gatcctcagg cctccatcgt
     7741 acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa
     7801 tgaagctgcc acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag
     7861 cgtctatcct ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc
     7921 cgcattcgtt tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc
     7981 caaacagaca gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct
     8041 ttcaagtgat aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac
     8101 tgttgcaggt gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat
     8161 caatacgacg atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt
     8221 tttcgtgcct tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt
     8281 tttattattt agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc
     8341 caggataatc cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt
     8401 ctaaatccga gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg
     8461 cgtaacttga ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac
     8521 ggatgtagtt ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg
     8581 ctgcagctac aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga
     8641 tcgcgttgat tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa
     8701 cgtgagatcc ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac
     8761 gttttgctaa ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa
     8821 cctcgaccga atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga
     8881 tgatcttgat tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc
     8941 cgtattcgcg aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac
     9001 gcgctggatg gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc
     9061 ttcggattgg atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat
     9121 cgataccagt cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac
     9181 ctttcatttc atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg
     9241 ctgcgcagcg ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt
     9301 tggcacgctg ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag
     9361 tcgatttcat gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt
     9421 ctttttgttg atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct
     9481 gttttgaact aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt
     9541 catctttacg attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt
     9601 ctaacttact ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct
     9661 tagcttctaa caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga
     9721 ttccagcagc tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca
     9781 taaaccctaa accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca
     9841 taaaatccac ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa
     9901 ttattctaaa ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact
     9961 ttattctaat gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta
    10021 cttattcatt aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt
    10081 taaattgaga aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta
    10141 tgtacccact cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg
    10201 tcaaggagag tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc
    10261 tactaaaggc aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat
    10321 accataagct gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat
    10381 atagatttta gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc
    10441 accgctttta tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga
    10501 gatccctaaa ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt
    10561 ttttacgact ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc
    10621 tgcacgacgt acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt
    10681 gatttctgga ttcccaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc
    10741 gattgttttt gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg
    10801 tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa
    10861 agcagcaact gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc
    10921 gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac
    10981 acctaatttt tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt
    11041 tccgccattt ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact
    11101 ttcaggtcca tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac
    11161 atctaacgca agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa
    11221 tttcataata gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc
    11281 tgctttacga tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat
    11341 ttcattatcc ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat
    11401 tcgcatttat cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc
    11461 attatatctg atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc
    11521 tttagatgaa atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc
    11581 agccactccg gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc
    11641 catctgctcc acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat
    11701 ctccagaaaa ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc
    11761 agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc
    11821 tgctgttaca gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt
    11881 atcatcgccg tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc
    11941 taaaagagct tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac
    12001 ttcatttggt ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt
    12061 tagctcagtg accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag
    12121 tttttcttca gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg
    12181 tttcaactca ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa
    12241 agtccctaaa gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga
    12301 ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt
    12361 tttgtatcct tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt
    12421 tgtcaggtca tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt
    12481 ttcagctaga gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata
    12541 ataaacttcg atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac
    12601 gacttgtcct agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg
    12661 ctcccttata tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag
    12721 ctgtgacaaa aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa
    12781 cctcggtcat aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct
    12841 tgtgactcag agttagacgg caggctcaca acctctccac taaacgatac ttacattgat
    12901 cctttgaaca caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc
    12961 agacaagcat aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta
    13021 ttgataaaca gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa
    13081 gctgcagcca tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat
    13141 tcacgacaga caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg
    13201 ataaaagcag tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta
    13261 gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa
    13321 gcaaaaataa ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat
    13381 tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta
    13441 agtacagtta atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt
    13501 gactatcagc gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga
    13561 gtatttttat tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt
    13621 cacgatcctt cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca
    13681 ttctgcgtat tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt
    13741 aactggtacg gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat
    13801 ggtcgcattt gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt
    13861 cccttgataa acataggcat tgtctactag cacacctaac catacacgat tgacagcagt
    13921 aaattcaagg gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga
    13981 atcttctcct gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac
    14041 tgtagaagac tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc
    14101 ttctacttta agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca
    14161 agtcatatag cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg
    14221 aagacttgtc caaaagcgac ttggatgcga ttcttcttga tgcattgctt ttctagaagt
    14281 acttactgtt tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc
    14341 aaaaacatcg actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa
    14401 tgcccgcacg taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa
    14461 ataacgtttt tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag
    14521 gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt
    14581 ctgtttgatt ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg
    14641 acaaaagtca tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc
    14701 ggtcacaagt cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg
    14761 actcggagtt aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc
    14821 ctcggtatta gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta
    14881 atactcaggt ca
//
LOCUS       DACEPW010000058        14709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEPW010000058 DACEPW010000000
VERSION     DACEPW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.059"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..156)
                     /locus_tag="IW819_001375"
     CDS             complement(<1..156)
                     /locus_tag="IW819_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1244486.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            844..1584
                     /locus_tag="IW819_001376"
     CDS             844..1584
                     /locus_tag="IW819_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244487.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(1676..2863)
                     /locus_tag="IW819_001377"
     CDS             complement(1676..2863)
                     /locus_tag="IW819_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1244488.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            3185..4027
                     /locus_tag="IW819_001378"
     CDS             3185..4027
                     /locus_tag="IW819_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1244489.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            4024..4974
                     /locus_tag="IW819_001379"
     CDS             4024..4974
                     /locus_tag="IW819_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1244490.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            4989..5222
                     /locus_tag="IW819_001380"
     CDS             4989..5222
                     /locus_tag="IW819_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1244491.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            5553..6137
                     /locus_tag="IW819_001381"
     CDS             5553..6137
                     /locus_tag="IW819_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1244492.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            6268..6936
                     /locus_tag="IW819_001382"
     CDS             6268..6936
                     /locus_tag="IW819_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1244493.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            6938..8362
                     /locus_tag="IW819_001383"
     CDS             6938..8362
                     /locus_tag="IW819_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1244494.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            8380..8949
                     /locus_tag="IW819_001384"
     CDS             8380..8949
                     /locus_tag="IW819_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1244495.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            9059..10813
                     /locus_tag="IW819_001385"
     CDS             9059..10813
                     /locus_tag="IW819_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244496.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            10767..12539
                     /locus_tag="IW819_001386"
     CDS             10767..12539
                     /locus_tag="IW819_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244497.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            13062..13331
                     /gene="rpsN"
                     /locus_tag="IW819_001387"
     CDS             13062..13331
                     /gene="rpsN"
                     /locus_tag="IW819_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1244498.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            13346..13525
                     /gene="rpmF"
                     /locus_tag="IW819_001388"
     CDS             13346..13525
                     /gene="rpmF"
                     /locus_tag="IW819_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1244499.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            13557..13934
                     /locus_tag="IW819_001389"
     CDS             13557..13934
                     /locus_tag="IW819_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244500.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            13955..14104
                     /gene="rpmG"
                     /locus_tag="IW819_001390"
     CDS             13955..14104
                     /gene="rpmG"
                     /locus_tag="IW819_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1244501.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            14128..>14709
                     /locus_tag="IW819_001391"
     CDS             14128..>14709
                     /locus_tag="IW819_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1244502.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQ"
BASE COUNT         5116 a         2077 c         2702 g         4814 t
ORIGIN      
        1 attttttttg aatgtattca tgaaatcttt gatatcctct acgtcaaagc gaactacgcc
       61 atcaattttt attactggaa aattatatac acgaatccac ttatctaaag tattattatc
      121 aattcctaga aactcacagg tttgtttttt attcaagtag actctcaatt ttataaaatc
      181 atctcctaaa aaagattaga atttttgaat tgctttgggt aagcacatag gaatttcacc
      241 tctccccagt ttacggcgag ccttacggaa gtatcattat ttgctgctat tgtcatggca
      301 ttttgttttc ccagaacaaa aagaaatctc taagtatttg tcgctaatca ggtcatggcg
      361 tacttgagtt tctagctcga atagcaggga tcgttattca aaaattgtat ttaattgtca
      421 atgatcaaaa ataggttttt tcctattcac tattaataac gataaacatg tataaaaaga
      481 cacactacga aaaaagttaa atggagttta ctgattttca tatttgtata tggaacagac
      541 atagtcttga ataattttta taaatgtttt gatgcttatc aacttattca acccgctatc
      601 cactttaagt ataatttttc catacgccaa taaagtagtt ttaggagcat gttctcattt
      661 gagaacgctc cttttttata tattttgaaa gctacttact atacgcgtag tttcaaatag
      721 ttttgtcatt ctaatcttca gcaacagaaa aaaatctaaa ttataaaatt tagggttgac
      781 taaaatataa tttttgtata ccatatgatc gtgataatca ttatcattta gaaggagaaa
      841 gttatgttag aagtgaaaaa gcttaatgtt tgttataacg attttttagc attgaacgag
      901 atttctttaa aaatacaaga aggtgcttta acaggtatta ttggtccaaa tggtgctgga
      961 aaatctacat tgcttaaagc aatgttaaat attatccctc atcagggaga ggtctccatt
     1021 aataatgaag atatcaataa aaaactggtg aagattgcat atgtagagca aaaagctgat
     1081 attgatttta cttttccaat aaaagtaaaa gaatgtgtat ctatgggaac atatgccgga
     1141 atgaaagttt ttcaaagaat taaaaatgca gaatggcaaa gagtttcaaa ggctttggaa
     1201 aaggtcgata tgggaaaata ttctaatcat caaataggag aattatctgg aggacaattt
     1261 caacgtgtac ttttagcaag atgtcttgct caaaatgcgg atttcatatt tttagatgaa
     1321 ccatttgtag ggatagactt agttagtgaa cgaataatta tggatacgtt aaaagaattg
     1381 aaacatcagg gaaaaacagt gctaattgtg catcatgatt taagcaaggt aaagaagtat
     1441 tttgataata ttattctttt gaatcgaaat ctaattgctc acggaagtgt agaatctgta
     1501 tttaatgaag agaatttaaa aaaagcctat ggagatacta tatttattgg agagactaac
     1561 tatatagagt caagtaaaaa ttagccaaat caaaaaatat tttataacag ataattccga
     1621 aaaaagggta caacaaataa gcttcttacc attagttttt ttgaatgttc aatagctaat
     1681 cagaaacaat tatataaggc tcgtttttag tcaaaatagc atgaatcacg taacaaagct
     1741 tacgagaaac cgcacctaag gcagttagat gatgttttcc ttcggaaact tttttctggt
     1801 aaaacgcact aaaaacagga tcacattgag aagctactaa agccgccgaa aacaaggctt
     1861 ttcgcaaata tggagaccct cgtttactca taacattatg cgttgcctca tattgccctg
     1921 attgtgtcac ggaagcgtct atcccagcgt aagcaacgag ttttttcggg ttagaaaatt
     1981 tttgaatatc tccgatctca ccaagaatag tcgcaccgtt tataggacca attccaggaa
     2041 ttgtgagaat aacagaattt aaagtgtcca ttatgtgctt gatttctctt tccgtttcct
     2101 ttatttggct ttcaatgaat tttaattgtt caatcattga acgtagttgg aaggtgaacg
     2161 cttcttgagc aaattttact ccaaaggagt tagaagctgc ttgcttcaat tggtccgctt
     2221 tttctgcctt gattcggttc ctgcttaact cagctaagta ttgggccaaa gtttctgagg
     2281 aaatgttttc taagtcgatc ggggcactaa acgccagcaa taactcttta gaagcttttc
     2341 cgaaaacgcc ctgcttggta aaaatcgtcg cgtattctgg aaagacttga tccaatacgg
     2401 ctataatttt tcttttgaag tcgctcgctg tacctactaa ataagttcgg taacgagaaa
     2461 gttgtttcaa agaaaaaaca ttttcatcgg ataagaccgt ttcgacaaat gagccatagc
     2521 gcattaagtc agcaatcaaa acagaatcaa taatatcgtt ttttcgtttc cgtatttctg
     2581 tacctttacg ccatccgtct gtttgaaggg gattcacaac gtgaatcaaa taatctgatt
     2641 cgtgaaggta tgaaaagatg gacagccaat agtgccccgt ggcttccatc cctataacaa
     2701 agtcactggg tgctccggaa tagtcgacca ttcgctcaat taatgactct gctccttcag
     2761 tggtgttagg aaatgaaaaa ccttttagta atacctttcc ttcctcgttc atgatagaag
     2821 caacgtgagt gcgtttaccg atatcaatgc ctaaaaagta catgtatatc tgacctttca
     2881 aaaaaagaat tttggattga aatccactca actgaaaagc gtcactacct tgtacgagat
     2941 acggagtaaa gtaaggactt tcaacatcta actcatacgc aatccactta tcagagagag
     3001 gagccagtct ctcgagtgcg ggttcaatgg cccaaggggg gaacggctta gcctctcaat
     3061 ccaataaata tattatctca aaagaaatga gttaatacca aattaaaata acgatggttc
     3121 atagggttat ccaatcaaaa ccggaaggtt aaaaccctaa ctctaatata caagggggaa
     3181 taatatgctt caaaatttta ttaatggtct gtatgacttt cactttttac agaatgctct
     3241 aattacatct gtagttatag ggattgtttc tggaacagta ggttgtttta ttattttaag
     3301 aggaatgtct cttatgggtg atgctatttc tcatgctgtt ttgccaggag tagcaatttc
     3361 ttatatttta ggtattaatt tttttattgg tgcgattgta tttggtctat tagcttctat
     3421 cattattact tttataaaaa gcaatagtgt aataaaagga gatacagcaa ttggtattac
     3481 tttcagttct tttttagcat taggagttat ccttatagga gttgcaaata gctcaacgga
     3541 tttatttcat atcttatttg gaaatatttt agctgtacaa gatattgata agtggattac
     3601 aattggcgtt tctgttttag tgttggttac agttgttcta ttttttaaag aattattaat
     3661 cacttctttt gatccattaa tggcaaaagc aatggggatg aatgtcagtt tttatcatta
     3721 tctattaatg attctattaa ctttagtagc agtaacagct atgcaaagtg tgggaactat
     3781 attaattgtt gcaatgttaa ttacaccagc tgctacagca tatttatatg caaacagctt
     3841 aaaaactatg atatttattt cggcgacagt cggagcgtgc tcatcattgc taggtttgtt
     3901 cattggttat agttttaaca tagcagcagg atcaagtatt gttttaacag cggcattctt
     3961 atttgtaatt agttttctaa tctcaccaaa acaaaaattt ttaagaaaga aggagagaaa
     4021 gttatgatga gaaaatggaa agcagtactg ggaagtctgg ggattttgat tgctcttttt
     4081 atattcgggg catgttcaac aaatagtaaa gacaaagata cagtggcttc gaacgaaaaa
     4141 ttaaaggtag tagttactaa ttcgatttta gcagatatta ctgaaaatat agcaaaagat
     4201 aaaattgatt tacacagtat cgtacctatt gggaaagatc cccacgaata tgaacctttg
     4261 cctgaagatg ttcaaaaaac ttcaaaagca gatttgattt tttataacgg tgttaacttg
     4321 gaaactggag gaaatgcttg gtttacaaaa ttagtaaaaa atgcgaacaa agaggaaaac
     4381 aaagactatt ttgcagcaag tgatggcata gatgttattt acttagaggg tcagagtgag
     4441 aaagggaagg aagatcccca tgcttggtta aatttagaaa acggtattat ttacgctaaa
     4501 aatattgaaa aacagttagc ggaaaaagat cctgataata aaaaattcta taaagaaaat
     4561 ctagataagt atattgaaaa attggattct ctagacaaag aagctaaatc taaatttgct
     4621 tcaattccga atgataaaaa aatgattgtt acaagtgaag gatgctttaa atatttctcg
     4681 aaagcgtata atgtgccttc tgcttacatt tgggaaatca acactgaaga agaaggaaca
     4741 ccagatcaaa taaaacactt agttgaaaaa ttacgcacaa caaaagttcc ctccttattc
     4801 gtagaaagca gtgtggacga tagaccaatg aaaacagtat caaaagatac caatattcct
     4861 atctattcaa cgatttttac tgattcaatt gcagaaaaag gacaagatgg tgatagttac
     4921 tatgcgatga tgaaatggaa cctggataaa attgctgaag gcctttcgaa ataatttgga
     4981 gggtaggtat ggtacaaatt aaaacgacta ctatactaat ttatttacta gcaattcagg
     5041 aattatcaaa aagaaaaaag aaattaaaaa ataatgattt agcaaaagtt ctaagtgtta
     5101 gtccagcatc tgtaagtgag atgctggcta aacttagata tgaggactat ttagatatag
     5161 gatttcaatt aactgataaa gggaaaaaat ttattgacga ttacaaaaaa ggttttaagt
     5221 aaaagacttt atttttaggg aggataagaa aatggctatt ggagtgatta tgataggact
     5281 taccctaata gctggatata atttttttaa atctttctcg gattatttta gaaaaggtga
     5341 atgatttaga tgagtctatt aattcttgta ggattgcttt ttgtatcagt tattttgctc
     5401 tttctcatta gtttagctct aaggaatgag ttcagtaata gacgttaacg attgaaactt
     5461 caagtcctgt gtaagataat atagttaatt catcttatta ggaggaagta cccatagaaa
     5521 ccagagatta tattacaact caccaaaaat taatagaaag tggactaagc cattttgaaa
     5581 aagaaggatt tcagcaaact aagataaggc aactgtgctt gaccgcaggc gtaactacgg
     5641 gtgcttttta taaacatttt acttctaagg aagcgttatt tgaagaaatt attcagccgc
     5701 atattaatgc gcttattaat atgtataatg aagagtttac caaatacctc ttaaacttag
     5761 aaaatataga tatagagaaa agttggagag agaacttcaa tgaattagat ccatttatta
     5821 attatatttt tgagaataaa cgtatattcg atttggtttt attccaatca gctggaacca
     5881 aatatcaaga tattgttgaa aaaattacat catttgtgac aaaaaagaca gtctgttgtc
     5941 tagataagtt aaagactatc ggaatgatca atcaaaattt agtctttaat gaagaagaaa
     6001 tccacttcta catttactca ttctacgcta cattctatga tatattgaag catagctatt
     6061 caaaagaaaa gactatgcta ttaaccagaa gattgtatca attttctgtt cctgggtgga
     6121 ttgaattact gagttaatta aaaaaaagct tgcattgaga attgttctca gttatgcttt
     6181 ttgataagat cgcactgtta tctgaataac ggtgcatttt tgtcagctta agataacagt
     6241 gcgatcttat tttttggagg tgaaagagtg tcaaaaatca atccgttaac cttgattact
     6301 ttaaatattt tttttccggt cattattttt ttaggaaagg gaaatatatt tcaagtgggg
     6361 tgtttcatta ttgctacagt tttagtattg atgtttcgta aaataaagac atgtatttat
     6421 ttcatcttga tttattcagt tctgttttta gtaatgaaaa ttattgatgc tatcaagata
     6481 gagtttttta ctatattttt tgggacatcc ttatatattg tatttcgaat gttgccggtc
     6541 gtaatgatct catggttgtt ggttagtgtc tatactagca atgaattatt atcctcttta
     6601 gaaaaaatac atttaccgaa aaaaataatg ttaagtatta cagtagtgat cagattcttt
     6661 ccgacttatc gttctgaaat aagaatgatt aaagaatctt taaaaatgag aaacattcgc
     6721 ttgtcttttt ttcagcctct gcaatatttg gaatttctgt tagttcccgt tcttatacgt
     6781 gcaactatga ttgctgaaga aatgaccgct aatgcaataa caaaggggat agaatctcct
     6841 gtgaaaagaa gttcttacta taaaacaaac atgagcttat tagactatat catcctgttt
     6901 tccgttacta tttttttctt aagtattttg atttgaggtg aaagaattgt ttgaatttaa
     6961 caatgtttct ttttcatata aggatgttaa ggaacaagcg cttgttgacc ttaatttttc
     7021 aataaaaaaa ggcagtctta cattgattac tggttcttct gggagtggga aatccacact
     7081 attaaagtta atgaatggac ttattccaag actttatgac ggaaaattgg ttggggaaat
     7141 tttatttgaa aataactcgc tacaatcctt aacacaagaa agtatatcaa aaaatattgg
     7201 ctacgtaagt caaaatccta gaggacaatt ctttacaaca aatacaacga gtgagttagt
     7261 attttcgatg gaaaactttg gatttacaaa agagcatatg gataaaagaa tcaatgaatt
     7321 agctgaatta ttagatcttt caaatatatt agataaaaac attttttcgc tatcaagtgg
     7381 tgaacggcag aaaatttcta ttggatgtag tttatcgcta aactctaaat taattatatt
     7441 ggatgaaccg tcttcaaatt tggacttcaa agcgactaag aaacttgctg acttactttg
     7501 tgatttaaaa aataaaggat ttactattgt tatagctgaa catcgttttt actatttgaa
     7561 agaattaatt gatcaagttc tatttgttaa aaatagaact gtaaaaacta caactattca
     7621 gttgttaaaa actgaaaaca ataacgcatt aaggaaattt gacattttta atagttcgat
     7681 tgaagttccc tatatcgaaa aggtaagtgc agatgtatta aaagttgaag aaattactta
     7741 tgaaaatatt ctaaaagaca taaattttga tgtcaaagtt ggagatgtta ttggtttggt
     7801 agggaaaaac ggtgtgggga aaacaacctt actaagaata ctcatgaaga ttattcgccc
     7861 taccaaagga aagataatcg aaaataaaaa aaatttgtca tcgccgtttt tagtaatgca
     7921 agaaatggat taccaatttt tcacagaatc agtgaggagc gagttagccc tggggaatga
     7981 aatagttagt aaagatcagc aagaaaaaat tttaaaaaaa atggaacttt ataaaatgaa
     8041 agatcaatta ccgtttgatt tatccggtgg agaaaaacaa agattattgg tttctatagc
     8101 ttcattgtca aaaactaatt tatttctatt tgatgaacct acaagtgggt tggattatat
     8161 caatatggaa aggattgctg accttattaa agacttaaaa gaaaagggtg cagtagttat
     8221 agccactcat gatccggaat ttctttataa aacatgcaat agaattattt atttaaaaaa
     8281 tggtggaata aaaaaagata taaaacttag agtagaagat tcaactttga tagaacagat
     8341 ttttaatgac ataacgaaat agaattgagg agaatatata tgaaaacaaa agattatatt
     8401 tttattggtt tagctacagt tatttcccta gtaatttact ttttttcaat aatggttagt
     8461 agtattggtg gggcgtttgg tcacagcata tccccaggga tttttggatt attatctgga
     8521 atcatatttg tttatattag ctataactac cctagaaaag gaatttttac tatttacaca
     8581 attgttttat tgttattttt tacgttaatg ggcggggcgt acttaccttg gtttatcagt
     8641 tcgattagta tggcgatctt agctgatatt attctatctg tatttggata tgatcgagca
     8701 attcctcaag ttgtgtcttg ggttctaatg caactaggca gtgctgccgg acagtggatt
     8761 cccatatggt tctttacaga tcgttttcgt caagactgga tagatagagg acaatctgct
     8821 gcaacaatgg atgcaatgat ccactacgcg gtgggtatat ggggaattat ttcagtgtta
     8881 gttgtagcaa gtttatctat gattggtgta ttgattggaa gaaaagtctt aaaaaaatac
     8941 aaaaaataga acgtagttta ccttctatct gtttgtacat ctctaattta cgctattacg
     9001 gaaagcatta acagttgtaa acgaataaga gaagaaattg aatgcggggg aggaaaaaat
     9061 gaaagtatac aaaaaattat ttagttatgt ccctaaaatg aaatactacg gatggttagc
     9121 cactttattt tctgattttt ctgtcgtatt aacagtctat ggatattaca gtatttacaa
     9181 atttcttcag tctctaatta ttgtaggaga tgaatattta gctaagagtt atgcggttca
     9241 aactgttgct tggttaactt taggagcttt attttatatt ttttctggct tattttctca
     9301 tatccttggg tttcgcttag agactaattt gagaaaaaag ggaattagcg gtttaacaga
     9361 agcaagtttt cgtttttttg atttaaattc ttcgggatat attcgaaaga caattgatga
     9421 caacgcggct aaaacacata tggcaattgc tcacttgatt ccagatagtg ctcaagccat
     9481 agtaacacct gttttatcag ttgccttagg ctttttcatt aactttaaaa ttggattggt
     9541 tctaatcgcc atgttagtaa ttggtgtatt cttgttaaag aaaatgacag agaatacaaa
     9601 ttttattcaa aaatatcaag aatcgttaga tgtgttaagc tctgaaacgg tagaatatat
     9661 ccgcggaatt caagttataa agatatttgg tgctaaagta acatcattta aagccttgaa
     9721 taaagcaatt aacgattatg ctaagtttgc atataagtat tctttgagtg ggaaaacacc
     9781 ttttgttctt tttcaatggc tattttttgg catagtttgc attttgatag ttcctataac
     9841 cttttttata acttctctta ccactcccaa gatcttagct gttgacttgt tgatgttgtt
     9901 ttttttaagt ggaattattt ttgtttcttt catgagaata atgtatgtaa gtatgcactt
     9961 gtttgaagca agatatgcag tcgaaacact agactccctt tatttagaaa tgaagcaaga
    10021 ccaactaaaa tatgggaacg agacacagat taaaaattgt gacattgcat ttgaaaatgt
    10081 cagcttttca tacaaagaag agtgtgtttt agaaaatttc aacttagttt tagaacaaaa
    10141 aagaatttat gcattagtag gaaaatcagg aagcggaaag agtacgatag ctaaactaat
    10201 ttcaggtttc tacaatgtgg ataagggaca gattaaaatt ggtggtaagc ctttaaatca
    10261 ctattcaaaa gacacaatta ttaacacaat tgcttttgtc tttcaagata cgaaactatt
    10321 caatcagagt atttacgata acgtttctat agcaaatcct caagcgccag caagtaaagt
    10381 atttgacgcg ctaagattag ccggttgcga atcgatttta gaaaaatttc ccgaaagaga
    10441 gcatacctta attggtagta aaggagttta tctgtcagga ggagaaaaac aacgtatagc
    10501 tattgccaga gcaattttaa aagatgcaaa gattgttatt atggatgaag ccagtgcatc
    10561 tattgatcca gataatgagt atgaactaca aaaggctttt caatatttga tgaaagaaaa
    10621 aacagttatt atgatagctc atcgattatc cagcattaaa tctgtagatg aaattcttgt
    10681 cttgtcagaa ggaaaaatag tagaacgtgg aaatcataat gaattaatgg ctaaacatcg
    10741 agtgtataaa caattggtca ctaattatga gaatgcaaat gattggaggc ttgacaatga
    10801 aaaattttta taaaaaaaaa tttgctttaa ctgatcaggg cgcagaagct ttaactaaag
    10861 cttctatttc tagttttttt gtatactgca ttaatatggt accagcattc attataatga
    10921 tgctgataga cgaattagtt ttagaaaatg caaagccccg ttggctttat tttgcagtat
    10981 cgtttgttac tttgcttttc atgtattggt tgttagatag ggagtatgaa aacttatata
    11041 atagtactta taaagaaagt gcgcatttaa gagtgcaaat tgcagacgat ttgtcaaatt
    11101 taccattatc ctatttttca aaacataatt tatcagattt atctcaaact atcatgtctg
    11161 acgttgaagg tattgagcat gcgatgagtc atgcaatacc taaagccggc ggtatggctc
    11221 tgtttttccc ttttatttca gtgatgcttt tggttggtaa tgtcaaaatg ggattagctg
    11281 ttattttgcc aacgttattt agttttgtct taatcttgtt atcaaagaaa tcccaaacga
    11341 aagccaatac taaatattac gatactttga gagaaaactc ggaagaattt caagaaacta
    11401 ttgaattgca gcaagagatt aatagcttta atctatctaa aaaagttcaa gacagacttt
    11461 tcaaaaaaat ggaagagagt gaaaggattc atttaaaggt agaattaagt actttttcag
    11521 tcatggcctt atcctctatt ttctcatatg ttagtttagc agtagtcatt ctagtaggtg
    11581 ttcacttact gttaacgggt gaagtgacta tactctacgt cgttggttac ttactagccg
    11641 caataaaaat aaaggattcc ttcgattcta tgaaagaagc agtgcttgaa atattttatt
    11701 tagctcctaa aatacagcga attagagcta tgaaggaaac cagtatccaa gagggatcag
    11761 atagtcctct gaaatctttt gacattgagt tgcgagatgt ctctttctca tatgataaca
    11821 ataccccaat tttagatcat atctctttta cggctaaaca aggagaagtg actgcgttag
    11881 taggtgctag tggttccggg aaaactagta tcttgaaatt agtgtctaga ctgtatgatt
    11941 atgatgaagg ctgtatttta attgatgggt atgatattaa acgtgtatct ccagcttctt
    12001 tgtttagcaa aatcgctatt gtttttcaag aggtaacttt gtttaataca agtattttgg
    12061 aaaatatacg tattggaaat agtcaagcgt cagatgagga agtgaaaaaa gcagctcgtt
    12121 tagctaactg cgaagacttt attgaaaaac tacctgacgg ttatcataca ttggtcggag
    12181 aaaacggaag ttcactttct ggaggagagc gacaacgcct atccatagca cgggcttttt
    12241 tgaaaaatgc gccaatttta attttagatg aaattactgc cagtttagat gcggaaaatg
    12301 aaaaacgtat tcaggaaagt ttaaatcgtt taatacaaga taagacagta ctaattatat
    12361 cgcatcgctt gaagtctatt gaaaaggtaa ataaaattgt agtgatggat caaggaaaac
    12421 tagttgatca aggtactcat aatgagttgt atcgaaggtc agaaatatac aaaaatctca
    12481 taaagaaaac taaactatct gagaaattcg tctatgaaaa agaagctcaa tcacgatgat
    12541 gaaataggct ttaaattaac ctacaatttt tgggatatag ttataattct ttttcaaaaa
    12601 gaataatttt attaaacagc atctaatttt gaatacacga aagaatcagt atgattgaat
    12661 tagttcactt aatgtctatt cttataatat aaggaataat caaagtttta aaaaattggc
    12721 gataaaccag ttaaacataa aagattgaat aaaaagttat agtttctttc atattgccgt
    12781 tttaagtaga ggaaaagcta cttaaaacgg tatttgtgtt tgattggact aataaaagtg
    12841 aattcttatt tttcagagcc taaactgcat attcttttaa atgatttgat tatctctatg
    12901 taccgataaa ttcgttttca aatagttatc tattcatatg agttttattt catgttattt
    12961 attgttgaca agagcaactc ttgttggtaa agtatcagtg tcaaaacgta acgattacga
    13021 tttgaggtgt ctaaatcaaa ataaaaggag tataaaggta tatggcaaaa aagtcgaaaa
    13081 ttgctaaagc aaagaaacaa atggcgatga ttgaaaagta tgcagataaa cgtcaggaat
    13141 taaaagccgc aggagatcgc accgctctcg ctaaattgcc aagagattca aatccgaatc
    13201 gtctgaggct tcgtgatcag acggatggtc gtccaagagg gtatatgcga aagttcggta
    13261 tgtcccgtat taagtttaga gaacttgccc atcaagggtt gattcctgga gtaaaaaaag
    13321 caagttggta aaaggaggaa atatcatggc agtaccagca agaaaaacat ctaaggcgaa
    13381 gaaacggtta agaagaactc atcaaactgt agtaaaacca gaaatttcat ttgacgaagc
    13441 gaatggtgat tatagacgta gccatcatgt atctttaaaa gggtactata atggaaagaa
    13501 agtaattaaa ggagcttcaa aatagtttaa tatataactt ttaggaggga atcaaaatgg
    13561 aaattattta cccaccttta gttgaagaag gattaaagta ttatcttgaa acgacccagc
    13621 aatcgctaga taaatgcact ttttatcgtt cgatggtaga gagaggaatt attactgaaa
    13681 cgggtttacc gacccaacag gcaattgaaa atggattagt caaagattac tatgaggacc
    13741 aaggcttatc atttgatgat tttttaagaa tctatccaat ttttgaagag tatgacgagg
    13801 agttatttca atgtatcgat ggttattggg agatacctgt tgatatgaaa gaaaatttag
    13861 tttcacaatt ggaatctggc gagttaactt ttgaagatgc acaacaaatt caagcatatc
    13921 ttgaagatcg ataagtattg aaggaggaag atttatgcgt caaaatatta ttttggaatg
    13981 tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat aaacggaata accctgaacg
    14041 gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg gcaattttta aagaagtgaa
    14101 atagctaagt ggtaaaaagg tgacaaaatg ggcataccag ttacaatcgt ttctgggttt
    14161 ttaggggctg gaaaaacaac gctaatcaat caagccttgc aagtctcacc ctaccccagg
    14221 gaagaaatta ttataattga aaatgagttt ggtgaagttg ggattgatca caaattatta
    14281 gttcatagcg aggaacgggt gttacaacta aataatggat gtatgtgttg cagtcttcgc
    14341 agtgatttat tagcaatatt gatttcttta ctggaaacaa tagaagaaca tcaacaacca
    14401 atttcacaaa tcatcattga aactacaggt atagcagatc cccaaccaat cattcaaaca
    14461 cttttaacag cgcctcaact aagaggaaga gtatatatcg atagtttatt gactgttatt
    14521 gatagtgaca gttttgaact tattgaaaga gaaccacaag cccttaagca acttgtcatg
    14581 gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt tatctgcact aaaaaaatta
    14641 cagagagaaa tcaaaagtat taacccttta tccgatattc gaatgttcac acatagtgaa
    14701 acgattcaa
//
LOCUS       DACEPW010000059        14386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEPW010000059 DACEPW010000000
VERSION     DACEPW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.060"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            208..786
                     /locus_tag="IW819_001392"
     CDS             208..786
                     /locus_tag="IW819_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1244503.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            786..1715
                     /locus_tag="IW819_001393"
     CDS             786..1715
                     /locus_tag="IW819_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1244504.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1712..3448
                     /locus_tag="IW819_001394"
     CDS             1712..3448
                     /locus_tag="IW819_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1244505.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3511..4347)
                     /gene="pheA"
                     /locus_tag="IW819_001395"
     CDS             complement(3511..4347)
                     /gene="pheA"
                     /locus_tag="IW819_001395"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1244506.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4344..4853)
                     /locus_tag="IW819_001396"
     CDS             complement(4344..4853)
                     /locus_tag="IW819_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1244507.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4846..6141)
                     /gene="aroA"
                     /locus_tag="IW819_001397"
     CDS             complement(4846..6141)
                     /gene="aroA"
                     /locus_tag="IW819_001397"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1244508.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6154..7245)
                     /locus_tag="IW819_001398"
     CDS             complement(6154..7245)
                     /locus_tag="IW819_001398"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1244509.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7262..8428)
                     /gene="aroC"
                     /locus_tag="IW819_001399"
     CDS             complement(7262..8428)
                     /gene="aroC"
                     /locus_tag="IW819_001399"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1244510.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8430..9494)
                     /gene="aroB"
                     /locus_tag="IW819_001400"
     CDS             complement(8430..9494)
                     /gene="aroB"
                     /locus_tag="IW819_001400"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1244511.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9527..10549)
                     /gene="aroF"
                     /locus_tag="IW819_001401"
     CDS             complement(9527..10549)
                     /gene="aroF"
                     /locus_tag="IW819_001401"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1244512.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(11208..12182)
                     /locus_tag="IW819_001402"
     CDS             complement(11208..12182)
                     /locus_tag="IW819_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1244513.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(12163..14046)
                     /locus_tag="IW819_001403"
     CDS             complement(12163..14046)
                     /locus_tag="IW819_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1244514.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(14064..>14386)
                     /locus_tag="IW819_001404"
     CDS             complement(14064..>14386)
                     /locus_tag="IW819_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1244515.1"
                     /translation="ISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLG
                     RILASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLH
                     FKKL"
BASE COUNT         4006 a         3154 c         2602 g         4624 t
ORIGIN      
        1 aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa taaaaaaata
       61 caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg aaatggtgag
      121 gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt tttaaattac
      181 aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat cgatgatgaa
      241 cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg gtttcaatta
      301 atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca tgcaatcgat
      361 atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc gcggctatta
      421 aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga atttgatatg
      481 gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg attgagtcct
      541 gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa gcccaacaaa
      601 agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca gattgctgga
      661 caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt ctgttatata
      721 ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg atcacccatt
      781 cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt tcggaagaat
      841 ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt caaaagtttc
      901 gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca atccaagata
      961 tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc tatcataagg
     1021 gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac gagcagcaag
     1081 caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc aaagaacttg
     1141 agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg aaacatgagg
     1201 caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt gggaatgaag
     1261 aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag tatttcgaag
     1321 atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct gcaaatgcaa
     1381 aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa aattatgaga
     1441 aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc tatctctcag
     1501 ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa gtccgtatca
     1561 gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc tgtgagaaag
     1621 tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca ggaatgacac
     1681 cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag agcatgaact
     1741 ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca tcgtcatttc
     1801 tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc aatcgaatga
     1861 tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa tcgagaatac
     1921 attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg atccagccca
     1981 acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact ctctttcaaa
     2041 aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa gaacttttgt
     2101 cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg cgattgctca
     2161 gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg gattgactct
     2221 tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata cattgaatca
     2281 cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc tttttcaatc
     2341 tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga cgatcatcgc
     2401 atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac taacagtagg
     2461 tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc gccaggattg
     2521 tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg tgatttttcc
     2581 gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat ttatgtattt
     2641 ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc tagaggaaga
     2701 tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc tagggaatac
     2761 gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc aagacgaaaa
     2821 gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc attttattta
     2881 taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg aagcagttca
     2941 aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag aagaagtgat
     3001 tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga gtatgcgata
     3061 tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat tatctatgcc
     3121 gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc acaataaaac
     3181 gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat tcgaagtgat
     3241 tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc atttttcagg
     3301 aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg catatggctt
     3361 aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac cgcttcttcc
     3421 taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt tttattttaa
     3481 gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta ctgtcagcac
     3541 tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt ggagcgcatt
     3601 ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat attctcccaa
     3661 gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc agccaaaagc
     3721 tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga tgacactcat
     3781 ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc aaaaacgtgt
     3841 ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa catgctgtgc
     3901 tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta catacattgc
     3961 tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg tttctaaaaa
     4021 ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct ttgttgctgg
     4081 attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt cgacttctgg
     4141 atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat tctctacagg
     4201 aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag ggatggaagc
     4261 atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag tgaatgaact
     4321 ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat ggtatcgatg
     4381 atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc ttcataccaa
     4441 gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag agctaaaggt
     4501 cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc tgcctctaag
     4561 taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg gacaatccct
     4621 ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc tgactccact
     4681 tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa ttggatatgt
     4741 tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc taacccttta
     4801 ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc catgagcttc
     4861 tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga ttgaaacagc
     4921 ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt gcagcatcat
     4981 accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg tcggcccatg
     5041 gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta attcttctgc
     5101 caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt tgatgatcgt
     5161 gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa tcagacgcgg
     5221 aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac ttaaatcagc
     5281 cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct cctccaacac
     5341 atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca gttggctttc
     5401 cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac ctggaacagt
     5461 tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat cttccactgt
     5521 gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac gttctttttc
     5581 aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag atttgacttg
     5641 cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc ctgtcacagt
     5701 gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct cgcgtaaagg
     5761 ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca gagtagtcgt
     5821 aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg agtttcccat
     5881 gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa tcttgatcac
     5941 ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag tactcagaca
     6001 atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg ctccaaacat
     6061 gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc ttaatccatg
     6121 tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat gataactggt
     6181 attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa aactgatttg
     6241 cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca aagaaactcc
     6301 cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg gcacatcgat
     6361 aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact ggtctcgact
     6421 ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca tgatccattc
     6481 tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata tctgcttatt
     6541 gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag tgatatctcg
     6601 aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt gttctgcctg
     6661 attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg ttatacggtc
     6721 atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata gttcttgcag
     6781 tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg cattttcaaa
     6841 tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg gatgtccacc
     6901 gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg tactgctgac
     6961 atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa gataagctaa
     7021 ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac ctgtttcaaa
     7081 atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg tttttgactg
     7141 actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa gtgccataga
     7201 agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat ttgccccctt
     7261 tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac tcaacaagcg
     7321 aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca acattttccg
     7381 caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt gctttgtaag
     7441 gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga atgggtttca
     7501 tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag ccgcctagat
     7561 tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc atcacttctg
     7621 agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta aaggcattga
     7681 tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt acgtagcttc
     7741 ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc gtatctccat
     7801 ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga tcaacaactc
     7861 gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt ccaggtattt
     7921 ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca agttcttcca
     7981 gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt gcagaggacc
     8041 gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc aaatccgcgt
     8101 gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc acttctgcta
     8161 cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa gtgacaggtg
     8221 aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct ttttcgatct
     8281 tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga ttgatttctt
     8341 cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat tctggtccat
     8401 gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc tttgaatgtc
     8461 tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat gatattcaat
     8521 tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc tggatgactt
     8581 agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc agctgtcgat
     8641 ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat tccaatagct
     8701 actccttctc catgactgac taccccatat cctgctgtat tttcaattgc atggccaatt
     8761 gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc ttccactact
     8821 tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag aagatctttt
     8881 tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc tgctgatttg
     8941 atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag aacctcagga
     9001 tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc tttgtttgta
     9061 ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt agtcggaatt
     9121 tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag atcacccacc
     9181 attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc tgctaaaaaa
     9241 tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg tacgattgaa
     9301 aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc atatagtgga
     9361 gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt ttgaagccat
     9421 gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg gttatcaaca
     9481 tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa tcgagtgtct
     9541 gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt gagtaagtct
     9601 tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga tggatctcta
     9661 cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt acaagttccc
     9721 aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat tttttcatca
     9781 atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc cgaatcccac
     9841 gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca gcattaagcc
     9901 attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct gttttaccga
     9961 cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg atatcgctgt
    10021 actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact ttcattccaa
    10081 attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct tggaaagcat
    10141 atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc ccggctttgg
    10201 ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga ccagccatcg
    10261 tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta ttgccaggat
    10321 ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt tctactcctt
    10381 catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt ccaatcacta
    10441 ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata tgatcgacga
    10501 ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt gtttttcccg
    10561 ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa ggtggtttta
    10621 cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct tctttcctac
    10681 ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct ctacagtgaa
    10741 catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta tagatgaatt
    10801 tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata caaacctggt
    10861 ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa gtaatacgta
    10921 ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat gtaagaattg
    10981 tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaact ttttttaatt
    11041 ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga agaaaatagt
    11101 catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat agaataaagc
    11161 agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta ctgaataata
    11221 aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac atccgctccc
    11281 cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac cgttcgtctt
    11341 tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt gaatggtaaa
    11401 gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc ttcttgatct
    11461 ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt ttccatatgc
    11521 atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac tttataaata
    11581 ccttcgacac caccagccat ccgatccgga tatgtttccc ctgataggcc ttcatactca
    11641 aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa ttccggtaat
    11701 cctttttgtc cataataatg catattgaca agcattttac cctctccagc gatttggtag
    11761 gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat gatttcaacc
    11821 gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg caaatcgatc
    11881 tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc ggctcctaac
    11941 cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt tgctcgccaa
    12001 aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag cgattccagt
    12061 ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc taatgtacag
    12121 tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac tgtatttttc
    12181 ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa aaacgatccc
    12241 attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat agcgcatcac
    12301 ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat cccatatata
    12361 gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca ttccacctaa
    12421 cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg ctttattttc
    12481 aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat ctgaaatcga
    12541 atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat cggggtctac
    12601 ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt catttccaag
    12661 agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt ttgctcgatc
    12721 aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag gctcaaatgc
    12781 gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca catggattcc
    12841 tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg tccgtacgcc
    12901 gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta tagtgatggc
    12961 gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca tccgttttcc
    13021 attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg aatagtcaat
    13081 atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat ataaattggg
    13141 ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg tcatttgctc
    13201 ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta tcgctacatt
    13261 cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt tgccattttg
    13321 acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt gtcctcgagc
    13381 aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat cttccaagaa
    13441 tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt ccaaataagg
    13501 tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa aatgaccatt
    13561 caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt gttttccctt
    13621 tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt ctttagcagc
    13681 tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac ggcgaaatac
    13741 atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat ttgctaattc
    13801 aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt ttttataaaa
    13861 tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc catttaaaga
    13921 ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa catgatcgct
    13981 atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt ttatatttgc
    14041 tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc tgtttggaaa
    14101 tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc cgaaaatatc
    14161 cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag atatacttca
    14221 tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc ttcatcttgt
    14281 ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc caaacgataa
    14341 taatggccat actggatcac cggacgaatc gtttgatagg atataa
//
LOCUS       DACEPW010000060        14171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEPW010000060 DACEPW010000000
VERSION     DACEPW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.061"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..299
                     /gene="aspS"
                     /locus_tag="IW819_001405"
     CDS             <1..299
                     /gene="aspS"
                     /locus_tag="IW819_001405"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="catalyzes a two-step reaction, first charging an
                     aspartate molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; contains discriminating
                     and non-discriminating subtypes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1244516.1"
                     /translation="HTRELQEKVLETLGFTKEQMEEQFGFLLTALDYGFPPHGGIALG
                     LDRFAMLLAGEDNIREVIAFPKNGKAADVMSDAPSMVSPLQLFELNIDVTNIEE"
     gene            408..1283
                     /locus_tag="IW819_001406"
     CDS             408..1283
                     /locus_tag="IW819_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1244517.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            1549..3432
                     /locus_tag="IW819_001407"
     CDS             1549..3432
                     /locus_tag="IW819_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1244518.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(3507..3791)
                     /locus_tag="IW819_001408"
     CDS             complement(3507..3791)
                     /locus_tag="IW819_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1244519.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(3781..4515)
                     /locus_tag="IW819_001409"
     CDS             complement(3781..4515)
                     /locus_tag="IW819_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1244520.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            4644..5267
                     /locus_tag="IW819_001410"
     CDS             4644..5267
                     /locus_tag="IW819_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1244521.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            5459..6580
                     /locus_tag="IW819_001411"
     CDS             5459..6580
                     /locus_tag="IW819_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1244522.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            6587..9715
                     /locus_tag="IW819_001412"
     CDS             6587..9715
                     /locus_tag="IW819_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1244523.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            9734..12928
                     /locus_tag="IW819_001413"
     CDS             9734..12928
                     /locus_tag="IW819_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1244524.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(13074..13781)
                     /locus_tag="IW819_001414"
     CDS             complement(13074..13781)
                     /locus_tag="IW819_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1244525.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4798 a         2467 c         2960 g         3946 t
ORIGIN      
        1 ttcatactcg tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa
       61 tggaagaaca atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg
      121 gtattgcatt aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg
      181 aagtcattgc ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta
      241 tggtttcgcc tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc
      301 cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg
      361 aaaaacactt tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat
      421 tcgaaaattg ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt
      481 tgatcgcatc agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta
      541 ttactggtct agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac
      601 cggtatcaac aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa
      661 tcgggaagaa gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc
      721 atattttacc ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg
      781 ctgtgatggg actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag
      841 atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct
      901 acttcaatgc attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt
      961 acagtacgtt gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa
     1021 agaagatgca ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa
     1081 actgtatgcg tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata
     1141 agcagacagt attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa
     1201 tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc
     1261 gtttctatga ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag
     1321 acagacgttc gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg
     1381 cttttttatt tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat
     1441 gggatgatag aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat
     1501 acccgtatat tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa
     1561 aaaaggttaa aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg
     1621 gtgggtgtta tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt
     1681 ccgatttttt tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa
     1741 caaaatggaa aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg
     1801 gcacttgttt ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg
     1861 ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa
     1921 atcaataacg gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc
     1981 tggtctttag cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg
     2041 atggtatttg ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc
     2101 tccgccatct tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac
     2161 ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca
     2221 agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga
     2281 ggaatctcgc ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc
     2341 atctattacg gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca
     2401 gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc
     2461 aaacgaagct atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag
     2521 gtgactgata ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta
     2581 atcatcagtg aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt
     2641 cgtacattcc aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca
     2701 tgggttgttc caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg
     2761 aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa
     2821 ttagcagaag aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc
     2881 cttgataagt acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg
     2941 gcatttggtg attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa
     3001 gtagtggtag atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa
     3061 ttaaaggaac aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca
     3121 ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg
     3181 gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag
     3241 atgacgaaaa aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat
     3301 agtgactggt tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt
     3361 cacttagtca gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac
     3421 gtgacagaat gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt
     3481 ttttatattt accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt
     3541 gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt
     3601 gttgcttcgc ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt
     3661 tgaagacgag tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga
     3721 tcggttgtat atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt
     3781 tcatttgcca tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt
     3841 gatcaatggt ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa
     3901 caaaatattt gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat
     3961 tcgatagtct tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt
     4021 aaagcgtcca tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt
     4081 atgaatctca tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag
     4141 ccctttgaaa taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga
     4201 taagctgtct ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc
     4261 gatactacgt cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc
     4321 ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat
     4381 cttccctttt ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga
     4441 aaacactttg gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc
     4501 gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat
     4561 ttttattaaa aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc
     4621 ataagaaggg aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac
     4681 tattagcctt agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg
     4741 agaattacat actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag
     4801 ctgcagcacc caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc
     4861 tgcgatttat attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa
     4921 gcgtcattaa aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc
     4981 ccagtgggac aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt
     5041 ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca
     5101 agcgtcagag agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa
     5161 cggataaaga aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg
     5221 acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta
     5281 tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg
     5341 agggagagac gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt
     5401 gtcatgtaat ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat
     5461 gcgtttttta catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat
     5521 tgaagaccag cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga
     5581 ggcaatcgtg attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga
     5641 attatacaat gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat
     5701 atcaggaaat cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc
     5761 gaactatttt ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt
     5821 acaatttttc tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga
     5881 caatatccga acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt
     5941 tgattcagaa aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc
     6001 tgattcggaa acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac
     6061 accgtttgat tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa
     6121 tgctcgctat agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg
     6181 cgtatggatc gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct
     6241 gcatgatatt gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca
     6301 gtccgtcaat cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa
     6361 tatgatgaac cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg
     6421 tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt
     6481 agcagaagca ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg
     6541 gctcggagaa acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa
     6601 attacaactt aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac
     6661 agaagcaccg ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga
     6721 tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat
     6781 gcgttctttg tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca
     6841 gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa
     6901 cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga
     6961 aataaaacaa atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct
     7021 cgatgcgaaa cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt
     7081 ggtttcatca agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt
     7141 ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca
     7201 tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc
     7261 agcagcaggg tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa
     7321 acaaatcacg gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga
     7381 aaattcctac tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat
     7441 aaacaagcaa aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat
     7501 tgggtattat gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa
     7561 agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac
     7621 tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa
     7681 aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc
     7741 cttacgtagt aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt
     7801 gaatgccgag aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca
     7861 agaaaattta ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa
     7921 atatctagat gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct
     7981 cgcacagata agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct
     8041 attgaaagaa cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat
     8101 tgctcgtctt cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga
     8161 acaccccata caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga
     8221 acaacaactg gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag
     8281 tgaagcacat gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc
     8341 aacgaatata caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt
     8401 tcctgagata acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga
     8461 tagacgaaaa agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc
     8521 agaaaagctc gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca
     8581 gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag
     8641 taactgttta gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca
     8701 actagctgtt gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac
     8761 acagcttgat cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca
     8821 agaaagaata gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg
     8881 tgcatttgca caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca
     8941 tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga
     9001 gacattccca gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga
     9061 aataaagcaa gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta
     9121 cgatgaatgt ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt
     9181 acagcagctt tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta
     9241 tgtgcttcag gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt
     9301 aacgaaaaat cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac
     9361 cggattagaa atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct
     9421 gtctggcggt gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat
     9481 ccagacacaa gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc
     9541 attagatgaa gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg
     9601 aagaatgatc ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat
     9661 aagaattgat acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg
     9721 gagattggta actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata
     9781 cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc
     9841 agaagtcttg ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga
     9901 ttactgccaa tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga
     9961 actttattta cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga
    10021 accggtaaaa gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac
    10081 acaaaacaat cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga
    10141 gactaatcag tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt
    10201 ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac
    10261 agaaaaagaa acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt
    10321 tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt
    10381 attaggtacg aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc
    10441 agtagaacaa gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca
    10501 gataaatgaa caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt
    10561 caaagtggaa aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt
    10621 ctttatgcat tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca
    10681 acaaacggtt tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt
    10741 gttcaagaaa aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat
    10801 cggcgacgta gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt
    10861 tattttcttt ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg
    10921 tgaagtcatc tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt
    10981 gttacgtgac acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca
    11041 gaaaaatgaa actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt
    11101 ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg
    11161 agagttgtat ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc
    11221 atggctcgat attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct
    11281 gcaaagcttg ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt
    11341 tgattcagaa gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg
    11401 tacagaagaa ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt
    11461 ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca
    11521 tccagaaaga taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca
    11581 aaagcaaggt tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc
    11641 cgatgaaatg ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga
    11701 agaacggaaa agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca
    11761 ggcagaggtg agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa
    11821 agaacgtgaa gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt
    11881 aagacaggat ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc
    11941 acaaatggtc aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc
    12001 tcagcggttt gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt
    12061 gtttgcaacg attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc
    12121 agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt
    12181 gaaagacttg ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa
    12241 aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga
    12301 agcattcaag aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg
    12361 ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt
    12421 aaaagatcta ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt
    12481 tacagggatg ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta
    12541 tttaagaggc agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg
    12601 cgaaaaagat gtttttctga tttccttaaa ggctggggga actggattga atctcacggg
    12661 agcagatacg gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc
    12721 tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga
    12781 aggaacgatt gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa
    12841 agtgatccaa ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat
    12901 tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag
    12961 aaagcctttc taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat
    13021 tccattttat cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca
    13081 aagattgtct tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt
    13141 ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt
    13201 tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc
    13261 atatgaaatt ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt
    13321 gcttgagaaa tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt
    13381 ttttttgtct gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc
    13441 ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc
    13501 tcgtaaattt tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct
    13561 tttagtgatt ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt
    13621 acttttggga agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct
    13681 gcatctgaaa caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca
    13741 tacatcgtat catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg
    13801 ttttcattga ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag
    13861 ctgataaata aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat
    13921 atttcagttt ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa
    13981 tcattatggt agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga
    14041 tttatttttt taatacagtc tcaatataac tattcaaaca taaaataacg ttatttgatt
    14101 tatttgttta tagtaaattt tatttagtaa tcattgcttt aaaatactac gcttatataa
    14161 cagagaaaat a
//
LOCUS       DACEPW010000061        13402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEPW010000061 DACEPW010000000
VERSION     DACEPW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.062"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..661)
                     /locus_tag="IW819_001415"
     CDS             complement(5..661)
                     /locus_tag="IW819_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1244526.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            854..1132
                     /locus_tag="IW819_001416"
     CDS             854..1132
                     /locus_tag="IW819_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244527.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1151..2359
                     /locus_tag="IW819_001417"
     CDS             1151..2359
                     /locus_tag="IW819_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1244528.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2507..2872)
                     /locus_tag="IW819_001418"
     CDS             complement(2507..2872)
                     /locus_tag="IW819_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1244529.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3019..3792)
                     /locus_tag="IW819_001419"
     CDS             complement(3019..3792)
                     /locus_tag="IW819_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1244530.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3966..4826)
                     /locus_tag="IW819_001420"
     CDS             complement(3966..4826)
                     /locus_tag="IW819_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1244531.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4826..6244)
                     /locus_tag="IW819_001421"
     CDS             complement(4826..6244)
                     /locus_tag="IW819_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1244532.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6301..6750)
                     /locus_tag="IW819_001422"
     CDS             complement(6301..6750)
                     /locus_tag="IW819_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1244533.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7195..7458)
                     /locus_tag="IW819_001423"
     CDS             complement(7195..7458)
                     /locus_tag="IW819_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1244534.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7557..8852)
                     /locus_tag="IW819_001424"
     CDS             complement(7557..8852)
                     /locus_tag="IW819_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1244535.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8849..10120)
                     /locus_tag="IW819_001425"
     CDS             complement(8849..10120)
                     /locus_tag="IW819_001425"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1244536.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10285..11154)
                     /locus_tag="IW819_001426"
     CDS             complement(10285..11154)
                     /locus_tag="IW819_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1244537.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11446..13056)
                     /locus_tag="IW819_001427"
     CDS             complement(11446..13056)
                     /locus_tag="IW819_001427"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1244538.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3782 a         2829 c         2304 g         4487 t
ORIGIN      
        1 ttcatcaaaa gataatattg tcttctaaca agtccatatt caaaataacg atatgatggt
       61 ctttaacggt gataatacca tcttttttca gttgttttaa aattctgttt acactgctag
      121 cagttgagat gccacaaaat ttggcgattt cttcattcgt gatcgaaaaa tcgatcagca
      181 tcccttcttc tgtccgcaca ccgaataaat catataattc gtaaagttgc gtacaaacag
      241 ctcccagctt tccgtttaat agcatttgct gcattttttt catcgaataa agcagacgat
      301 agcggtaata ctcttttaca tagtgttgta gctttgggtc ttggttgata ttttgccaaa
      361 agcgcacacg attgattcga taaaactctg catattctga ttcaactcga atattatacg
      421 gcgaatcgat gaattgtgaa tattcgtctt ttaaaagaga gacgatttca agatctttga
      481 tatagcgaag gttaaattct ctcccatcat ttgaaataac acttgctttt accgttccct
      541 ctttcaagat atacgtatgg gaatcttcca tcccttcgaa tgtcaaatat ttatgatatc
      601 ctttgcgtat aatcggaaaa tcatttttat ccaaatattc ctttaagact ctactatcca
      661 atgatgctcc cccatccaaa ctttcatcta aaaagatcat acagtacttt aaaacttttt
      721 aaagtccaaa ggtattttac taatgatatt aacatttgtt aagtaccttt ttccatcact
      781 tatgcgattt ttttcctttt ttttattcgt tatactgcag aagtaaaatt ttttaagaat
      841 agagggaaaa aacatgaatt actggatgaa cacaatcatt aatcgattag aaacagcata
      901 tcaaacaaga tttgatatga aagcttcttt agtcttttta aatgatgcgt atcaaaactc
      961 gatcgaattg atcaaagcag tggatgagaa tccaacaaac gaatgcgaag aatttttaaa
     1021 tctgtttatg tctacaagag atttatttat tcgccaactt gttgatcgct atccatccaa
     1081 ttatcacgat gtagaagttc aaatacaaaa attaaaagca tattcagctt aaggggaatg
     1141 tataacatac atggaaaaga aattatttaa taaatatttt attggaatca cattgatcaa
     1201 ttttattgtc tacctgattt attatctgtt gatggtcatc atggcagtca ttgcgcaaga
     1261 caccttacac gccagcttga gccaagcagg ttttgcttct gggatctata tcatcggaac
     1321 attgctggca cgcttattca tggggaaaca gttagaacta tttggtcgga agtttacctt
     1381 aagaggcggc gcgatcttct atctcctttc aacattagca tatctttata cgccaactat
     1441 cggggcattg tatttagttc gattcttgaa tggattcggc tacggtacag tttctacagc
     1501 tacaaatgct atcgttactg catatatccc tgcatctaaa aaaggggaag gcatcaatta
     1561 ttatggatta agtactagtt tagctgccgc tgttgggcca tttttaggaa tgattttgct
     1621 gaatatgact gattttcgtt tcattatctg gttttcaatt gtactggtct tctttgttac
     1681 gattgcctgc cttgtctttc ctgtaaaaaa tattccatta tctgaagaac aaaaaatatc
     1741 tcttcgctca tggaatatca atagttttat tgaaaagaaa gcacttttca ttacaggaat
     1801 tgcctttttg atgggtcttt cttattcaag tgttttatca tttttatctt cttatacaaa
     1861 agtaatccac ttagttgctg ctgggtcatt tttcttcgtc gtatatgctt tggtcatcac
     1921 atttacacgt ccattgactg gaaggatttt cgatgtcaaa ggagaacaat acgtgatgta
     1981 tcctagctat ttattcttaa cagcaggatt atttttactg agtgtaacga cgaatagtgt
     2041 gacattgctt atctctggag cattagtcgg cctaggttat ggtacgttta tgtcaaatgg
     2101 acaggctgtt tgtttaaaaa tcgtccgcga acatcgaatc agtattgctt tatctaccta
     2161 ctttattggt ttagacctag gcttaggcgt tggtccttat ttgatgggga tgctaaaatc
     2221 tcttacttca tttagaggat tgtatgtgat tgccggagtc attccattga tatgtacagc
     2281 actctatctt gttaatggcc gaaaacatag tcatgaagca gagggcgaat tgattgaatc
     2341 attgaaaaat gaaatttgat cttgatgaat agataaaaaa aaccgttcag atctgaaaat
     2401 aatctgagcg gttttttgag taaaaatagt ttatgattat tcttaattaa acatctattt
     2461 atttttaaag ctttcattta tcgataagaa tagtttaact gttgatttat tccattttcc
     2521 gttcatctgg tacgatgcct tgacttagca accacttgtg gaagaaccat tctgccacac
     2581 cgatcaatag agcaggaatc aatactttca tccaaatatc gactgtatca ttcataaaat
     2641 atagatagag ccacaagata actgcagctg aaccagcatc tgcgatcgtc gctgcgatgt
     2701 tccctacttt acgtaagatc aacagatccc ctagcacata gatcatgact acaataatta
     2761 ccccaacaat cgttgaatcc ataaagctaa caccgtataa acctgtcagc acgacccaca
     2821 gaatcgccca gatcatgatt gccttcacta ccaacgcctt cacatgtttc attttctgca
     2881 ctcctttctc ctggataaaa aagtaagatt atctaacaag ataacctttt cattattata
     2941 ccatcggctg agagaagcga gcaaagataa ggtcctatct aaaaatgagg aagccactta
     3001 gaacattcac gtaataattt atccgtacag acattcgata tggtgttcct ttactttcgc
     3061 atactgttct tttgtcagtt ctttacttgt caccagataa tcgatttcat gaaaagcaat
     3121 ggcttgatgc atgaatttat gtgcaatttt ttgctgtgta actaaaacgg caagccggtc
     3181 tgattgtttt ctaaaagctt ttttgaccat tgcctctcct tcatgagttt ctgttactcc
     3241 ttggctgaaa tctagcccgc gacaactcat tatcgctaaa tccgcataat atgacaatgc
     3301 atcgttatag gcttttgaac cgttgattgt tgctcgtttc ggaacaattg cccctcccaa
     3361 tacagatact tttgccgttg tatattcact caacatagca gctgtgacga tgccattcgt
     3421 aatgatctgc aaaccatcaa atcttgctag gtttctgact aagtgtaaag aggttgtact
     3481 ggaatccaag aataagacca tatcatctcg aatcaaagaa gaagctgtat ctgcaatctt
     3541 acgtttttct gtttcttctt ctaatttggt gatcttcatc aggtaaggca cgtcaatttt
     3601 tcgattgtca ataagcatgg cgccaccgcg aactctacgg atcaatccac ttttttccag
     3661 aaaaacaagg tcacgtttaa tcgtggcttc gctataattt actaactcga tcaaatcggt
     3721 gactcttgca aattggttgt tttttagata attcacaatc tcctgctgtc tattctccac
     3781 ttcattcaac ataaaatcac cccatttact ttatatagta acaggaaaaa agaggttgtg
     3841 acagaagtga ttggcttcga gaaataagaa gaaatttttc gaaaaaacta cttctgaaca
     3901 tcgtgtgtgg ttaagagggt atgacaagac tattgtcata ccctcttgac cacacatttt
     3961 tatttttagt ataaactagc cttgcctact gtgttcaaaa tttccatttt atgtttgacc
     4021 acttcttgta cctttttatt agctgctgga taaattgcat tcggctcgta catcgatgga
     4081 ttttcactca atatttctct caaagcattg aaaaagacgc ttttaagatc tgagctcaaa
     4141 ttcactttgg caactccgta ttttactgct tcgctgactt ctgcgtccgg attacctgaa
     4201 ccgccatgta aaacaaatgg aagatcgatt tgttggttca attctttcag taagtcgata
     4261 tttaattttg gagttctgtt tttcggatac aagccatgcg ctgttccaat tgctacagca
     4321 agtgtatcga ttcctgtttc tttcacaaaa atcttcgctt gatcaggatc tgtataaatg
     4381 attgtttcag cacctccttc agcagaacca ttattcccaa tcgttccaag ctcagcctcc
     4441 actgagacat ttaccgcatg agctaacgca accacttttt tcgtaatctg tacattgtcg
     4501 tcgaatgatt tcaaagacgc atcgatcatc acggaagaat agccgtttcg aatcgcccgt
     4561 aagacatctt ctatcgtacc gccatgatcc aagtggataa cgatcggcag tttacttgta
     4621 taagcatagg ctcttactgc cgcaacaaac tcatcactta aataggcgat ttcatcagga
     4681 tgaatttcta agattactgg tgcttctaat ctctctgctt cttcgatgat tgctttcatc
     4741 atatcatagc tgcaaatatt gaaggctggt actgcaaatt gattctcttt agccactttc
     4801 aataactctt tcattgtata taacattacg cttccacctc atttgttaga ttttcatttt
     4861 tcgtccactt gtcttgtttt ttctctttca ctgttttttt caatagtcca agcataaagg
     4921 ctgtagtgac tgtacctgat gcaattgcca agacatacat aggtaaattg ttcataacat
     4981 ttggaattag catcacccaa atccctccat gaggtgccat tgaagtaact cctactgcca
     5041 tggaaatccc tgcagcgact gctgaacctg tcatcaaact aggtatcacg cggatcgggt
     5101 cagataccgc aaacggtata gcaccttctg taatatacga agcgcctaaa atccagcatg
     5161 attgacctga agtccgttct tctttggtaa atttatgttt gaataataaa gtggctaaag
     5221 caatacctag gggaggtgtc attcctgcag ccatacaagc agcaatcggt gcatttactc
     5281 cagctgacat caaaccgatc gagaaagtcg aagtcgattt attgatcggc ccacccatat
     5341 cagctgccat catgatcccg ataagtaatc cgaacaatag cccggatgta tttcccaagc
     5401 tattcagcca atttgtcaaa gcatccaaca cccatgtgat cggttgttca atcacaaata
     5461 ccatcaaaag cccaataatt aagatcgata aaaaggggac gacaatcagc tgataaatag
     5521 cagaaaattc tttttttacg ctaattttat ttgccagaaa atcacagata taccctgcta
     5581 gcacgccacc aatcattccg ccaagaaaac ctgaaccacc tgtagaggcc agtaatccgg
     5641 caacaattcc tggggcaaaa cctgcttttc cagcaatcga tgtagcaatc cccgcgccta
     5701 acgcaggaac catcatggca aacgctgtat ctcctccgat ccttgaaaat gcagctgcta
     5761 acacattata gtctgctgag ttgggatcag ccgctgtaat cccaaaggca aaactcagtg
     5821 cgatgatgat tccgcctgcg atcacaaatg gaagcatgta attcacacca ttcatgaaat
     5881 gattatagat tgtcggcttt ttctcatttg cagctggttc tttttcatta gacacaacga
     5941 atacgccaat atctctcaat gcttcgtcta ttactacttg cggttctttg attgctcggc
     6001 ttgtagagac acgctttact tttttcccgg caaatcggtc caattcgatt tctttatcta
     6061 ctgcaagaat gattgcatct gcctctttga tttcttgttc agataactga ttttctgttt
     6121 ttacaccttg tgtttcgaat tttgcttgat ggtttagatt tcttgctgtt acttctaatt
     6181 tttctgccga catataagta tgagcaattc cagtcgggca agcagttaca ccaacgattt
     6241 tcatgattat tttcctcctt tttcttcact atcgatactc ggacaaagac tctactattt
     6301 tcattcttga ctaatctgtc tgatgatatc tgctggcgta gtggcttctt ttattccctt
     6361 tctaaaatct gcattcatca atcgtctgga gagtgtgctg agaatctcta gatgcatatc
     6421 tgctgatttt tcaggaacta cgatggcaaa aatatattca attggcattt catctatccc
     6481 gctatagtcc gttaatttgg ctgtatgaaa aaaacacagt gctggttttg tcacatattt
     6541 gctttttgca tggggaatcc caaatccatc ttctattcct gtggagatct cctcctctcg
     6601 tttgaatagt gccttaacga ctttttgaac atttcttacc ttatttgttt ttttcaacac
     6661 atcccctatt cgctcaaata atgatccttt tgaatctatc gatggatcga aaacaatcag
     6721 ttcttctgcg atcaattgat caaacctcat aaaaaacctc tcccccacaa aaaatgttaa
     6781 cgttttcttg gaactgaggt cagtatagca tcgctagctg taccgttcaa tattatgaat
     6841 gaacagagga aaaagaccca tcatccgcgt gtataagtca ttaaaatgaa cgatctagct
     6901 cacaaacaat cgcaagtcta taacaaaaca aaaagccttc cttgaaagga aagcttagct
     6961 tctaatttta ggtaaatggt tttttacttc gaaataaccc ctcttcattt cgttaatata
     7021 aggtgttttt atcaagtgga taattaaaac tatatactag tttttcattg ttttgtgcat
     7081 tatgatccta cgactttttc agcatcaaac gaagttttaa attggcaaaa aacataaaaa
     7141 aacagactat ccctatcaga tagtctgcta aacgttgagt tatcagcgtt tttattattc
     7201 cgcagcgttt gcgtctttga gaccgtattt tttgttgaaa cggtccacac gtccgtctgc
     7261 ttgtgtgaat ttttgacgtc cagtgtagaa tggatgtgag tcagaagtta cttcgacacg
     7321 gatgactggg tatgtattac cgtcttccca ttcaactgtt tcttgtgaag tttttgttga
     7381 acctgacaag aatttgaaac cagttgttga atccatgaat actactgggt ggtaatctgg
     7441 atggatgttt tctttcatga ttttttcgct cctttgccct gctccatttg ctgaaccaga
     7501 gttgatttgt cgatttacaa cattaacatc ttaacatgtt tcagtcaagg ttgcaattat
     7561 cttttcagat ttctggtcgg atttttttta ccaaaggaga catcttgaaa ttctttgaaa
     7621 aattgctgat tgttttttgt tttacgcaaa aagcggatca gttgttccgt atattctaat
     7681 gaatctccag tcatattttt tcgaagtttc caaatttctt ctaattcttc acttggcatc
     7741 aataattctt cttttcgggt acctgatttt ttgatatcga tggctgggaa gatccgacgt
     7801 tcagataatt cacgtgaaag ttgtaattct agatttccag ttcctttgaa ttcttcatag
     7861 atcacgtcat ccatacggct tcctgtatcg actaaagcgg tcgctaaaat cgtcaaactg
     7921 ccaccttctt cgatgtttct tgctgccccg aaaaatttct taggtttata caatgcggct
     7981 gggtcaatcc cgccactcaa tgtacgtccg cttggtggaa cgactaagtt ataggcccga
     8041 gcaagtctag taatactatc catcaagata acgacatctc gtttatcttc gactaaacgc
     8101 attgcgcgtt ccaatactaa ttcagaaact cgagtatggt tttgcggctg cagatcaaat
     8161 gtagaagata ccacgtcgcc tttcacgctt cgttctagat ccgttacttc ttccggtcgt
     8221 tcatcaatca gtagaacgat taattctact tccggatagt tttcggtgat accatttgcg
     8281 atttctttta atacagaagt tttccctgct tttggcggtg cgacgatcaa tccacgctga
     8341 ccaaaaccga ttggagaaaa gatatcgatc attcgggtag atagtctttg cggtgttgtt
     8401 tccagttgaa gctgtctttg cggataaaga ggtgttaagg ctggaaaatg cggacgttct
     8461 tttgcttctt ccggatcttt tccattaacg ctttccacgt gcatcaaacc ataataacgt
     8521 tccgattctt taggtggacg ggcttttcct gccactttgt ccccatttct caaactgaat
     8581 cgtctgattt gtgaggcaga aatatagata tcttctgcac ttggtccata attgattggc
     8641 cgtaaaaaac cgtaaccatc ttgaccaacg atatccaata ttccttccat ataataaaat
     8701 ccttgtttct ctgcttgtgc acgaatcaca gctagagata attccttttt attcatctta
     8761 ctgtaataag gaattttaaa ttctttggca taactataaa cttcttttaa cgttttgttc
     8821 tctagttctg ccattgtcaa ataatcgctc attcagctcc cacttcctct atcatttcaa
     8881 ttgatgcccc taaggctgtt agtttctcga taatatgatc ataaccgcgt aagatataat
     8941 ctacattgta aatcgtcgtt gtcccttctg ccaatagtcc agcaatcact aaacaagcac
     9001 ctgcccgaag atcagaagcc acgacttctg tgccgtgaag tttatttgga ccattcacga
     9061 tgatcatatt tccttccacg gtaatatcag cgcccattcg tgctagttcc ggcacatggt
     9121 tcacacgttt cgcatagatc gtatcgatga tttctgaagt gcctgtagtc atcaatagta
     9181 acgcagtaag tggctgctgc agatctgtgg caaatcctgg ataaggatag gtcatgatgt
     9241 ttgcgggttt taattctttc gaggggtaaa cttcgatttc atcttcgctg attttcatat
     9301 ggacaccaat ttcctgcaat tttgctataa agctttccaa atgttcaaag atcacatttt
     9361 tcactttgat accatttcct actgcagctg ctaatgctag ataggttcct gcttcgatgc
     9421 ggtcaggaat catgaaatgc cgacagccat gtaatgtttc gactccttcg atacggatca
     9481 catctgtacc cgctcctcga acttttgccc ccatattatt cagtaaagtt gccacatcta
     9541 tgatttccgg ttctcgtgca gcgttttcaa ttactgtctg tccttttgct tttacagcag
     9601 ctaacatcac attgatcgtt gctccgactg aaaccacgtc catgaaaatc cgattgccac
     9661 gcaatccttt attttctgtc cgtaaataca tggcaccatg ttcatttgtc acttcagcac
     9721 ctagagcttc aaatcctttg acgtgtaggt caatcggtct agggcctaaa taacagccac
     9781 ctggcaggcc tactaccgct tcgccaaatt taccaagcag tgttcccata aaataataag
     9841 aagcacgcaa actattgatt tttccgtgcg gcatcggaat cgatacgact tctcgaggat
     9901 cgatttctag tatgttgttg gaaaaatgga cagttgcgcc catgatctct aaaatttcaa
     9961 ttaatgaatg aacatcttgt atgtccggca caccttccaa gatcactggg gaatcagcta
    10021 agattgcagc tggaatcaat gcgactgcac tattttttgc accgctgatc gttacttcac
    10081 cagctaaagg tcggtttcct ttgattacga tttttttcat ctgtttccac cttgtttatt
    10141 aatctttttt catggatatg cttgtatttt atcatagata aaaaaaagaa acgagcatgg
    10201 tctgccgttt taagtaaaaa agaatggaga gcgacttcaa aagtcgttct ccattcctat
    10261 atatcgtatc tgttatgtgg atgcttacgc tttgttagct gaaccgaacc agtcgatatg
    10321 agatttagca tcttcaacga ttgctgcttt acctggagct aataatttac gagggtcgaa
    10381 tcctttacct tctttatctt taccagcttc gatatattta cgagtagctg cagcaaatga
    10441 taattggaat tcagtgttaa catttacttt tgagataccc attgagatag ctttttcgat
    10501 ttgatcttga ggaatacctg atccgccatg taatactaaa ggcatgtctg gacctacagc
    10561 atcagcaatt gcttgtaagt ggtcaaaagc taatcctttc cagttttctg gatattgacc
    10621 atggatgtta ccgataccgc aagctaagta gtcgatacct gtagcaacca tttgtttgca
    10681 ttcttcaata tcagctaatt cgccgttacc gatgattccg tcttcttctc cgccgattga
    10741 accaacttca cattcaactg agataccttt tgcatgtgct ttttcaacaa cgtctttagc
    10801 taatttcaag ttttcttcaa atggtagatg tgaaccatcg aacattactg aagtatagcc
    10861 taattcgata cattccaaag cagcttcgta atcaccatgg tctaagtgga tagcaactgg
    10921 aacagtgatg ttcattgctt caactaaatc agtgattaaa tctttacaga ttttgtaacc
    10981 acccatgtat ttagctgcac ccattgatgt ttggataagt actggtgctt gttttgcttc
    11041 agccgcttca aggattgctt gagtccattc caagttgttt gtgttgaatc cgccgacagc
    11101 gtaaccgcct ttacgagcag cttttaaaaa ttcagctcct gatactactg gcataataaa
    11161 attcctccta agttttgtaa aactaaattt gttaagacaa ttcttaacca acgaccctat
    11221 tttaccaaac atttcggtag ttttctacta aaatcaatga aaatgaaaat tttcaagaag
    11281 ttttttgcaa aaaataaaat atccaatagc gctttaatcg aaaaaagtct tttgctttct
    11341 atcttttttc ctacaaataa taacaaatca gaaaaagaga aaagtgacaa gaaaattgct
    11401 tatccccttc tcttgctact tttctctttc tatcaagttc aaaaattatt ttgtttcgtg
    11461 atctgctaat gatgctccga caaatgcttt gattaatttt tgcgggcgat ttggacgaga
    11521 aatcaattct gggtggaatt ggcatcccac aaagaatttt ttctctggca gctctacgat
    11581 ttctactaaa cggttatctg gtgagacacc tgaaaagact agcccttgtt cttcaaacaa
    11641 ttgacgatat ttgttattga actcatagcg gtgacggtga cgttcttgca caacatcggc
    11701 tccatcatat gcttcagccg tcttcgttcc ttttttgatt ttacatgggt ataatccaag
    11761 gcgtaaagtt ccacccaaat tctcgatgtt ttcctgatca gccattaaat caataatatt
    11821 gtttgctgta tctggattcg tttcagctga tgacgcatct tctaatccaa cgacgttacg
    11881 tgcaaattcg acacaagcca tctgcatacc aagacaaata cctaaaaacg gaacgtcatt
    11941 ttcgcgagca taacgaatcg cttcgatttt cccttctact ccgcgatcac caaatccacc
    12001 aggaaccaag attccatctg cgtctttcaa acgttcttct acattttctc tcgttaattc
    12061 ttgtgctttg atccaatcga tctcgatgtc tgaattatag gcgaaaccag aatgtttcaa
    12121 tgcttcaact actgagatat atgcatctgg taactcgaca tatttaccga caagcgcaat
    12181 ccgtactttt ttcttcaagt tcaagacacg ttcttctaat tctttccatt ctgtcatgtc
    12241 tgcttctggt gtatctagtt ttaaatggtc gcaaacaatt tgatccattc cttgtgcttg
    12301 taaaactaat gggatggagt aaagtgtatc tacatctggc gactcgataa ctgcttctgg
    12361 ttcgacatca caaaattgtg ccaatttgtt tttcacgctt tgagaaacag gttgctctgt
    12421 acgtaccacc aaaatattcg gttggatacc taaaccacgt agctctttta cactgtgctg
    12481 cgttggttta gttttcattt ctccagcagc tttcaagtaa ggaatcaatg ttgtatggat
    12541 atacataaca ttatctgtac cgacatctgc tttcatttga cgaagtgctt caaggaatgg
    12601 caaagattct atatccccta ctgttccccc tacttctgta atgatcacat ctgaatcagt
    12661 cattttagct gctcgcatga ttttttcttt gatctcattt gtaatatgag gaataacttg
    12721 aacagttgct cccaagtatt cgcccttacg ttctttacgc aaaacttcag aatagatttt
    12781 tcctgtcgtc acgttggagt atttgttcaa attgatgtca ataaaacgtt cgtagtggcc
    12841 cagatccaaa tctgtttcag caccatcatc tgttacgaat acttctccgt gctgatatgg
    12901 gctcatcgtc cccggatcca cgttgatgta tggatcaaat ttttggatag ttacttttaa
    12961 gccgcggttt ttcaataagc gtcctaaaga agctgcaaca atcccttttc cgattgatga
    13021 aacgacaccg ccggtcacaa aaatatattt tgtcataagt tcaaaaactc ctttttaatt
    13081 tatgtgcagg aaaagtctca gacaataaac aaagctccct attcttcaat aagaataggg
    13141 agccgaaatt tctgtaaact tctgaccctc taaaagggtg cccaaggtgt ataatacaag
    13201 ctgactaagg gaaagtcaag tactcaaatg aatactatcc gcttacaatt ttttcttttt
    13261 cgatatcctt tgagaaatta tttcttaagt cctttatgag tgcaaaaaaa atttaaccca
    13321 cataataagc ttgatataaa catagctaac gttaaaccta agcaaatcag aaaatcgtta
    13381 ctgaaaaatt ttaagtgaaa ta
//
LOCUS       DACEPW010000062        13212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEPW010000062 DACEPW010000000
VERSION     DACEPW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.063"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..1016
                     /locus_tag="IW819_001428"
     CDS             147..1016
                     /locus_tag="IW819_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1244539.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IW819_001429"
     CDS             1225..2250
                     /locus_tag="IW819_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1244540.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IW819_001430"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IW819_001430"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1244541.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="IW819_001431"
     CDS             3333..5036
                     /locus_tag="IW819_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1244542.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IW819_001432"
     CDS             5038..7071
                     /locus_tag="IW819_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1244543.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IW819_001433"
     CDS             7082..7522
                     /locus_tag="IW819_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1244544.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IW819_001434"
     CDS             complement(7722..8849)
                     /locus_tag="IW819_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1244545.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IW819_001435"
     CDS             complement(8852..9529)
                     /locus_tag="IW819_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244546.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IW819_001436"
     CDS             9793..11559
                     /locus_tag="IW819_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1244547.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IW819_001437"
     CDS             11574..12011
                     /locus_tag="IW819_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1244548.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IW819_001438"
     CDS             12024..13175
                     /locus_tag="IW819_001438"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1244549.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DACEPW010000063        13188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEPW010000063 DACEPW010000000
VERSION     DACEPW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.064"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            153..1892
                     /gene="yfcC"
                     /locus_tag="IW819_001439"
     CDS             153..1892
                     /gene="yfcC"
                     /locus_tag="IW819_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1244550.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2049..3233
                     /gene="iadA"
                     /locus_tag="IW819_001440"
     CDS             2049..3233
                     /gene="iadA"
                     /locus_tag="IW819_001440"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1244551.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3344..3547
                     /locus_tag="IW819_001441"
     CDS             3344..3547
                     /locus_tag="IW819_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1244552.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3648..4310)
                     /locus_tag="IW819_001442"
     CDS             complement(3648..4310)
                     /locus_tag="IW819_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244553.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4323..5825)
                     /locus_tag="IW819_001443"
     CDS             complement(4323..5825)
                     /locus_tag="IW819_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1244554.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6373..8052
                     /locus_tag="IW819_001444"
     CDS             6373..8052
                     /locus_tag="IW819_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1244555.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8179..8658
                     /locus_tag="IW819_001445"
     CDS             8179..8658
                     /locus_tag="IW819_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1244556.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8877..9557
                     /locus_tag="IW819_001446"
     CDS             8877..9557
                     /locus_tag="IW819_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1244557.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9565..10896
                     /locus_tag="IW819_001447"
     CDS             9565..10896
                     /locus_tag="IW819_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1244558.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10911..11873
                     /locus_tag="IW819_001448"
     CDS             10911..11873
                     /locus_tag="IW819_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1244559.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMLMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11984..12322
                     /locus_tag="IW819_001449"
     CDS             11984..12322
                     /locus_tag="IW819_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1244560.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12401..13108
                     /locus_tag="IW819_001450"
     CDS             12401..13108
                     /locus_tag="IW819_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1244561.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4203 a         2323 c         2888 g         3774 t
ORIGIN      
        1 aataatcata cataggagaa aaaaaacaaa atagaaaaaa gtgattttct aataaattat
       61 tcatgtggaa atgtcatttt tactgtataa ttcatttgat tatccatttt ttattcatca
      121 aaattactga aaaaagaaag gaagaaataa atttgaagaa aacaaattct tcaggaaaac
      181 cgaaacgcga cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag
      241 tcatctttgc atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata
      301 ttgaatacaa agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt caagattcct
      361 tccgttatgc ttacgagctg gataagtcgt atgtgtttga tcaattagaa gaactgcaag
      421 atcatccagc agaacgtgaa aaactggatg ttccggaaaa aggattggaa aaagtcattg
      481 ctgacggaga aaaaaatctt acgcaggaaa aactagatga aatctccttg acagatgatg
      541 tgctttacga tcagtacggc gaaaatatct atgatacttc taaaaaattg cataaaacag
      601 ccaaaatatg gggaacagat gattttggcg gctttggttt cttaaatttt gtttttgaag
      661 gactagtatc tggagataag tatggatctg ctgtagggat cgctgcattg atccttgttg
      721 ttggaggtgc ttttggtatt attatgcgta ccggagcgat cgacgcaggt atctatgcgt
      781 ttatcagtaa aactaaggga ctggagcgtt tagctttacc gctattattc tttgcttttt
      841 cattcggtgg cgctacattc gggatggcag aagaggtaat tccattctca atggtgatgg
      901 ttccatttgt cattgcgtta ggatatgact cgatcgtagc agtaaccgtg acttatgtcg
      961 catctcaagt cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg gtggctcaag
     1021 ggattgctgg aatccccgtg ttgtctggtg cgacattccg attgattatg tgggtagttg
     1081 ttactgcatt agccgctggt tatatgatga tatatgcaga aaaaatccgt aaaaaaccag
     1141 aaagttcgtt gacttacaaa tcagatgatt atttccgttc tcatatcaaa aagacatctg
     1201 atgaaaataa aaaattcatg cttgggcata aattgatctt attagagatg ttagttgtcc
     1261 ttgtctggat cgtttggggc gtgactcaaa aaggctatta cattccagaa atcgcttctc
     1321 aattctttgt gatgggatta gttgctggaa tcattgctgt tcttttcaaa ttagatggaa
     1381 tgaaagtcaa tgacatcgct tcttctttcc aatctggtgc agctgattta gctggtacag
     1441 caatcgtagt aggtatggcc aaagggattc ttcttgtctt gggaggatct gatgcatctg
     1501 ttccttcagc tttgaatacg attttgcatg ggatcgggac ggcgttgacc ggtgttccag
     1561 ctgtcattgg tgcttgggcg atgtacatct tccaaagttt gttcaacttg gttgttacat
     1621 caaattcagg acaagcagct ttgactatgc caatcatggc tccactggca gatttagtag
     1681 gtgtatccag acaagtagca gttcttgctt atcagcttgg ttcaggtttt atggacgctt
     1741 ttacacccgt atcagccagc ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa
     1801 aatgggcaag attccaaatc aagatgcaag gattcctgtt tgttttagga actattttca
     1861 tcatgattgc cattgcaata ggtctgcagt agtactggag acctgtaaat aaaaaagtaa
     1921 tccaaacagt agataaggac tccaagaacc gtcattttac ggtaaaatca aaggagtaaa
     1981 gatcctatcg tttggattat tttttaagat cctatcatca gttatactta aagaaagagg
     2041 aaaaatgtat gaaaatcatt cgacaaattg aagtgtttgc accagattac ttaggaaaaa
     2101 tggatgtact gattgccggt gggaagattt tggctgtaga agaacagcta gaaggcggct
     2161 acgaaggagt ggaagtagaa gaactttcag gtgagggaaa agtactaacc ccgggtttca
     2221 ttgactgtca tttccatatt ttaggtggag gaggagaagg cggttatcaa aatcgaacgc
     2281 cagaagtcac attgagccaa ttgactacag ctggtgtaac gacagttgtc ggttgtttgg
     2341 gaacagacgg agaaggtaga gatatgactg cgttgatcag caaagcgaaa ggtctggaag
     2401 cagaaggaat ctccacttat gtatacgaag gttcgtaccg attgccggta aaaacagtaa
     2461 cagattctat catcaaagac tttttgacaa ttgataaaat tatcggaatc ggagaaatcg
     2521 cagtatccga tcaccgttct tctcagccaa gttttgaaga gttcgctcgt gcagtagcag
     2581 atgctcgtgt aggaggcatg ttatccggaa aagctgggat catcaatgtc cacttaggcg
     2641 gaggaaaaag aaagctagag ctattgacac gaatcgtaga agaaacagaa attccgatca
     2701 cacaattttt gcctactcat gcaaatcgca caccagaatt atttgaagct tgcgtggcat
     2761 ttgccaaacg tggtggcacg attgacttca ctgctagcga agatcctgat ttttgggaga
     2821 agacagatgg tgaagttcgc ttcagcaaag cactgaaacg attgatcgag gaagatgttt
     2881 ctttagataa tttcacgatg acctcagatg gacaaggaag cttgccttat tttgatgaaa
     2941 acaatcattt cctagggtta ggagtaggaa gtgccaaggc attgctagta gggatcaaag
     3001 aagctgtgca aaaagaaagt atccctttgg aaatcgcctt acgagctata accagtaatc
     3061 cagcacgtat attgaaattg gataaaaaag gaaaaatcga aattggtgct gatgcagatc
     3121 tgtgtatctt ggataaagaa acgcttgata ttgatacagt tatcgctaaa ggagaaataa
     3181 tggtccaaga aaaagaagtc aaagtatggg ggactttcga aaaaagtttt taatcaacag
     3241 ctctaactat cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta
     3301 ccctgaaggt agatcaataa acaaactaag aggaggaagc acaatggcag atttaaaagg
     3361 acgttctaaa gatatgaaag ataaagtagt cggtgaagga aaagaagcat acggcaaagc
     3421 aacagacgac aagagcaaag aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa
     3481 agaaaaagct cgaaatttag gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa
     3541 aaaataaaaa ccacttatta ctaattgaat caagcaaata aaaaatccga accatatctg
     3601 cgagacagat atggttcgga ttttttattt gatcattatc ctgtttttta aacttcgatt
     3661 ttttgaaatt ttttattttt tagttcaaag acttcatcac agctttttgc tacttcaatc
     3721 tcatgagtca cgatgataat acagcgattt tgttcatggg cgatttcttg gaaaagtttg
     3781 acgatatctt gtgaagttgt ttcatctaga tttccagtag gctcatccgc tacgattagt
     3841 tgatgttcac agcagatagc acgaacgata gcgactcgct gctgctgacc tcccgaaagt
     3901 ttggtcacct gtttttttgc taatatttca tcaattccta cacgagccag tcgatctaaa
     3961 gcaacttgtc gataattctc ttgcttggtc ttagtgattt ccattgctgc tatgacgttg
     4021 tctagagccg ataaataagg aagcaagttg taagcttgga aaataatcga gacatcattt
     4081 ttacgataac ttcttaaccc gattttgttc agtgaacgat cttgatagta tacctttcct
     4141 tcttttggtg catctagtcc agaaataagc gaaagaaaag tcgtttttcc agaaccactt
     4201 gctcctaaaa tagcgtacat ttttccagct tcgaaattca gttctacatc tttataaagc
     4261 gcatcttgcg gatcttgata ccaaaatccc aattgttctg tccgtaacat caaattcctc
     4321 ctttagttta ataggatttt tcttgggttt aatcttaata tccctataga agctaaaaag
     4381 acagaaaaca gactgattcc aaatgctact ccggctaata gtcccaattg tgcaggtttg
     4441 acattgattt ccaattcgct gacttgatca gaaacagcaa atggattatt cgtggttcct
     4501 tgttgaccat cgcgttgacc gccttgttct tgtctgttac ctggcatttg tccaccgcca
     4561 ggagcttgct ggttttgtgt cgtggtttct gtctgttggt caagtagctg ctgtcccacg
     4621 gcattcgcga cgacgtttcc gctaagcgaa gcaactccta atgcaagaat catacaaata
     4681 gccacttcgg taaacaattg gagaatgatt ttacttctgg attctcctaa agatagcaag
     4741 acgcctaact catgccgacg ctcccgaatg gtaatcataa tgatcagagt aagtatgata
     4801 ataccggcta cagccactag taaaacaaca tttttagaaa aacttgccac gttgtttaaa
     4861 ggttccaaca ttgactgata catggaatca tttgattgta agctaaatgt atctgtgtcg
     4921 atcagcttct cagcttcttt gagaaactca tccattttat ctggatcaga tagagtgtaa
     4981 atagcagaat ctatcgtgtt atcgtcagat gtcccattta gttcatttgc aaaagtgtaa
     5041 gaagtgtaaa gtgtattcga cggattcatg aagttgaatt tcatacccat actgccgctt
     5101 gtttcgctgg attcataaat tcctttgatc gtcatttcat aagtggtatc ttcatcttca
     5161 gtactcgtga ttgtgaaagt atctccgact gccaggtcat ttgtttctgc tagagtagaa
     5221 tcaatgacta cattgtttgt gcctttgtcg ctgtcatcta ttccttcacc atccgtgatt
     5281 tttgctgttc catcagaaaa attactattt tgtgcagttg ctgacacacc actgatttga
     5341 aagtctgctt gagtcatttg cggattcccg cccattcctc cagcaggttc accgcctgca
     5401 tcagtagttt ctgaactgtc ttcacttgaa tcagaactag tgatggcagt tattccatct
     5461 tttgccatag ctgaggcgga actttcaaag gaataactct ttacattatc taattcagct
     5521 attttttccg cgtccgttaa ggaaactggt gtaagagaaa aactgcctgg atctggacgt
     5581 tcttcagtgg tagaaccgct tgaagaagtt tgttcctgtt ttttaaatga agcttctcga
     5641 ttggcactta aagtcactgt tgctccaact tctttttgtg cttgattcgc tgcttcttct
     5701 gcagcgcttc gaatcgtgag gccagctagc acaaaaataa gaatggctgt gaacacggca
     5761 aacaataaca atgaacgtcc ccattttact tttgtacttt tccatgcacg tttcataaaa
     5821 ttcatatttt gcatccctcc tagtagatgc atttgataat agacaaccta tcttaaagct
     5881 agcttaaatc ttatgctgtt tgcacaccat caacatgagg ttctgtatat ataaaaagtc
     5941 actatagtta ttgcaaaaaa tgtggtaaaa tgaaaaaagt atgtttcaaa ccgacgaaga
     6001 agaaatgaaa gaggaatagc aaaagaaatc ctagatgcta cagagggatt tttacgtgca
     6061 tagttgttct aacaattgct gatactagcg atttagttta tttgttgtat aataatgggg
     6121 aacagctgaa tacttatata agtattctgg ctatagcgtt tcgcaaaaga tccattttgt
     6181 taatgaagta tatccaaaat aaaatgccca tccaactgtc agaacgcgga tgtgctggta
     6241 attttcctgc tgattctcca taagttgaga aatagtgatt ttgcatatgc ttcattggaa
     6301 tggctcatat atttagataa agaagaaaat cgacatcttt aaacttggat gtcataagaa
     6361 gaggtgaagt ttgtgagtac cattaaaatt atcccattag gcggcgttcg tgaaaatgga
     6421 aaaaacatgt atgtcgcaga agtgggcgag gatattttta ttttagattg tgggctaaaa
     6481 tatcctgaaa atgagttgtt ggggatcgac gtcgtaatcc ctgattttac gtatttagaa
     6541 gaaaacattg atcgtgtagc tggtgtcttt ttaacacatg gacatgcaga tgcgatcggg
     6601 gcacttcctt acttgttgtc aaaaatttct gtaccagtat tcgggacaga gttgacgatc
     6661 gagttagcaa aattgaatgt cagtcgtaat gaagcagcca aaggatttaa ggatttccat
     6721 gtggtagatg agcatacaga aatagacttt ggtgaaacag tagtgagctt tttccgtacc
     6781 actcatacga taccagattc agtggggatc aatctgaaaa caaaagaagg tagtatcgtt
     6841 tatacgggag attttaaatt tgaccaaacg gctatcccaa tgtatcaaac agattatgca
     6901 cgattggcgg aaatcggcaa agaaggagtc ttagccttac taagtgaatc ggcaaatgca
     6961 gaaaatcctc agcctgtagc ttctgaagcc caaatagcag atgaagtatt tgatacgatc
     7021 aaatattggg aagggcgtat catcgtcgct tgtgtagcaa gtaatttgca acgagtgcaa
     7081 caagtattgg atgcagcaga aaaagcacaa cgaaaagttg tcttgactgg tcaagatttc
     7141 gaacgaatta ttcgcacagc gatgcgttta gaaaaacttc aattaccaag tgaagatctg
     7201 cttgttacgt tgaaagacat gaaaaactat gatccagaag aactgatcat tttagaaact
     7261 ggccgaatgg gtgaacccat caagtctctg caaaaaatgg caaacggtac acaccgtaat
     7321 gtcaagattg aagaaggaga tttggtttat atcactacta cgccaagcat cgcgatggaa
     7381 accatcgtgg cgaaaacgga agatatcatt tatcgagctg gtggaacagt gaagttgatc
     7441 tctgagaata tgcgcgtttc tggccatgcc aatcctaatg acttgcaatt aatgatcaat
     7501 ttgatgaaac caacatattt tgtgccagtt caaggagagt atcgtgaatt agctgctcat
     7561 gcagatttgg ctcatgcagt agggatgcca tacaaaaata tctatattac tggacgcgga
     7621 gacgtgctgg aatataagaa caagcgtatg agtgttgcag gaactacaag tgcagaaaat
     7681 gtaatgattg atggattagg cataggtgat atcggaaaca ttgttttacg tgatcgaaaa
     7741 atcttgtcag aagacgggat tttcgtagcg gttgtcacta tcagtcggcg agaaaagaaa
     7801 atcatttcaa aacctcaaat tacttcacgg ggattcgttt acgtaaaagc tagccgtgat
     7861 ctgatgaaag aaagcagcaa tatgattgaa acaatcgttg agaaacattt agaaagcaat
     7921 gattttgagt ggagcaaatt aaagcaagac atccgagatc aattaggccg ttacttattt
     7981 gaacaaacaa aacgccgccc agtgatcttg ccagtcatta tggaagctac tcaacgtcga
     8041 aataaaaaat aaacaaaaag agtaagatga acctctctat agagtgctcg tcttgctctt
     8101 tttttgcgaa aaacaaataa tttttgaata cagcatccct cgctttataa taaacgatag
     8161 aataaaggga gtgaatcaat gatgagtcaa acaaaagtcg aacgttattt agaagaacaa
     8221 aagatcagct atcaaccact tgattttagt catgtggtag aagaatcttt cacagaagaa
     8281 ctacaaaaaa gagggattga tccagctttg atatgtaaaa ctctcgtagt gaaaggaaac
     8341 aaaactgggg tgatcatcgc attgattcct ttgaacgatc acttagatta caaaaaaacg
     8401 agacaagtga cgaaagatcg taaagttggt tttcctggaa tggattttgt attaatgcat
     8461 acaggatatc cccacggagc aaacacccct attggaataa aactcgcaca tcctgattat
     8521 tgttttttag cagacagttc tttaaaagaa aaagaagaag taattgtgtc aagcggtgaa
     8581 atcgggagaa gccttcggat aaaagtagcc gatttagaaa agatcgtcca tcctatttac
     8641 gctgatttaa ttagttaagg gacgagaaga gaagctaagc agagcagaga cctcaaactt
     8701 gagattatat ctgcttggta cttggtgcat cctgtattat ctgttacaat tgctttgtac
     8761 atactgtact atcaatccaa tgaaaatata tttagcagct atattttttc attgatgtgc
     8821 aagtgatgaa atcgttttta ataaaaaaga tacagagaaa acgcgaggtg taaaatatga
     8881 aaattacgta tcatggacat tcctgtttaa caattgaaat ggaaaatggc caacgcgtga
     8941 tgattgaccc gtttattaca ggaaatccat tgacagactt aactgcagaa gaagtaaaga
     9001 cagattggtt gctgattact catggtcata gtgaccacat aggagatatg attcctattg
     9061 caaaagaaaa tcaagccaca attatcagta ttgttgagat tgctgattat gcgaacagca
     9121 gaggagtaga ttcattcggt atgaatatcg gtggaaaaca tgcgtttcca ttcggcgtgg
     9181 taaaattcgt acatgcacag catagttcaa gctatgaagt agacggtatc gttcagtata
     9241 tgggcgagcc tagcggaatt attattcaag cagaaggaaa aacgatttat catgcagggg
     9301 atactgctta tttcagtgat ctaggattgc ttgcagaaga attcgatatt gatgtcgctt
     9361 tcttgccgat tggcgataac tatacgatgg ggccagaaga tgcagcaaag gctgcgcaaa
     9421 caattcgtgc caagaaagta gttccaatcc attacaatac tttccctgtc attgaacaag
     9481 atcccgaatc ttttgtagag ttactggaag atagtgaagg aaaaataatg agcgtctcgg
     9541 aaacgctgga agtgtaggca gaaaatggcg acaaaacatg atttgatttt agaatatatt
     9601 gaaagtttac ctgttggcaa tcgaatttct gtaagaagca tcgcaaaaga attgaatgtc
     9661 agtgaaggaa cagcgtaccg tgcaatcaaa gatgcagaaa atgtcggact agtttcaaca
     9721 attcaacgag tagggaccat ccgtatcgaa cgcaaattaa aaaaacatat tgaacgtttg
     9781 acatttggcg aggtcgtcag gatcattgaa ggggacgttt taggcggggc ggcaggatta
     9841 gacaaggtct tgaataaatt cgtcatcggc gcgatgacta aacatgccat ggaacgctat
     9901 attacgccag gatcactaat gatcgtcggc aaccgggcgg aagtccaaaa attagcattg
     9961 gaagatggcg ccgcagtatt gatcacagga ggattcaata cgactgctga gattgcaaag
    10021 ctagctgatg atcttgaaat gccgatctta cgcacctctt atgatacatt tacagtcgca
    10081 accatgatca accgagcgtt aagcgaccaa ttgatcaaaa aagatatcat gcttgtcagt
    10141 gatatctata tgccagctga aaagactcat tatttgcagc agacagatac gatttctgat
    10201 tatcagcgtt tgtcagaaga aacgcagcat tcgcgttttc cagtcgtcaa ccgccatcat
    10261 cgtttgatgg ggattgttac ggcaaaagat gttttaggga aatcgccaaa ccagatcatc
    10321 gatcgggtga tgacgaaaga accaatcagt gtaaagaaaa caatgagtat tgcctctgtt
    10381 agccatcaaa tgatctggga tgggctagaa gtcatgccgg tcgtttccga tgacttgaca
    10441 ttggaaggct tgattacccg gcaagatgta atgaaagcga tgcagcttgt ccagcgacaa
    10501 ccgcagattg cagatacgat ttcggatcag atatctggaa atatccatac gattgatact
    10561 gaccgtgaag gaaatcgttt ggaaaaccct aagttcaaat tcgtagtagc accacaaatg
    10621 gtcaatggcg ttggaaccat ttcttttggc gtacttagcg aaatcatttc tgatgtcgca
    10681 caaaaaacaa tggtgatgaa ccaaaaacga aatatcttga ttgaacaagt gaacctacat
    10741 tatttgcggc tgatccaatt ggaaagcgaa ttagacattc gtccacgtgt attggaaatc
    10801 ggcagacgtt ccgctaaact ggatatcgaa gtgtttattg aaaacaccat cgtttccaaa
    10861 gcaatcgttg tttgtcaagt aatggaacgt tcttagaaag caggagtgaa atgacagtac
    10921 agcaagaaat agtagataca ataaaaaaat acgataaaat cattatccac aggcatttgc
    10981 gccctgatcc tgatgcatta ggatcacaag taggattagc cgaactatta cgggaaagtt
    11041 tcccagaaaa agaaatacgt caagtaggtg aaacgattga agggcttcgc tttttagcag
    11101 aaatgcaaga agttgaagat gtcttctacc aaggagcgct agtcatcgtg actgatacag
    11161 ctaattcacc cagaatcagt gatcaaagat atcagttggc agaaaaactg atcaaaatcg
    11221 accatcatcc caatgatgac ccttatggag atcttgtgtg ggtaaataca aaagcaagta
    11281 gctgtagtga gatgattgca gaatttgctt tgatgtttcc tgaatcttta aagatgaacg
    11341 ttaaggctgc acggttactt tatgcaggaa tcgtcggaga tacagggcgc tttttgtatc
    11401 cctccacgac gtcccgtaca ttagagattg cttcgatatt gagaaattat ccatttgatg
    11461 caagtgcttt gaacagagag attgaacaaa tgctgatgaa agtcgctaaa ctttccggtt
    11521 atctttatca aaatatccag gtggatgaaa atggtgcagg aagaataatc cttccgcaag
    11581 aaattcttga taaatatgga atcgatgatt cagagacagc ttcgatcgtt tcacttcctg
    11641 gagtgattga cgaagtgctg gcttggggga tttttgttca gcagccttca gggtattacc
    11701 gcgtacgttt acgttctaag gggccgatca tcaatgaatt ggctaaacgc catcatggag
    11761 gaggccatcc attagcaagt ggtgcgaatg caaaagattt agcagaagta gaggaaatct
    11821 ataaagagat ccaagatata tgccaatcgt ataagagcag aaaaacagaa tgatacttcg
    11881 ttcgaaaacg tgccttttgt agtaaaaatc acaaatgtat cttttcaaga tcttcttttt
    11941 ccgttacaat agtgctcact aaagatttta aaggagaaag aagatgacat taccaaattg
    12001 cccagaatgt ggctcagagt acacatatga agatcgaggc ttgttgattt gcccagaatg
    12061 cgggaacgaa tggaacaaag aagaacaaga aactgtcata gaagacggat tagtggtatt
    12121 agatgcaaat ggaaaccgtc tgttagaggg cgatagcgtg acagtgatta aagacttgaa
    12181 agtaaaaggt gcgtcaggtg ccattaaaca aggaacaaaa gtcaagaaca tccgtttagt
    12241 agaaggcgat cataatatcg attgcaaggt agaaggattt ggaccgatga aactgaaatc
    12301 ggaatttgtg aaaaagaatt aaagaatatt caaggtcttt tcacattctt ttcctactac
    12361 taatgataaa ataaagtaaa cgaaaaggag tgaaacgaaa atgtatggtt atggaatgta
    12421 tggtttttta gatccgacat ttcttttggt cattcttgga ttagttattt ccggaattgc
    12481 gtcagcgtac gtcaatagta ctttccgaaa atacgatcaa gtgagaagca aaaacaatgt
    12541 gactggtaca caagcagcac aatatatatt acaaagtcaa cagataaata atgtcggtgt
    12601 tcaacaaatt gccggagatc tcacagataa ctacaattca ggtaataaga tgttgagttt
    12661 gtctcaagca actgcacagt ctacttcagt tgctgcgata ggtgtagcag ctcatgaatg
    12721 cggacatgcg gtacaagatg cttcgggtta tgtcccattg aagattcgtg cagcacttgt
    12781 tccagcagct aactttggtt cgatgatttc gttccccttg attatggtag gtgtattgct
    12841 tagctggaac tctacgttaa tcaatatcgg gattttcgct ttttctttag cattactttt
    12901 ccaattggtg acattaccag tcgaatttaa cgcgtctcgg cgagcaatcc aaattttgag
    12961 tgaaggcgga ctactgacgg aagaagaagt accgatggca agacacgtat tatttgcagc
    13021 agcactgact tatgttgcag cagcgctttc gacattcctg cagctgttgc gcttgatctt
    13081 attgttcggc ggcaaccgaa gagattaaaa taaagaagtt gtgaaatcag cgctttgatc
    13141 tgagctaata ccgagagtta gctgattgaa taccatttat ttggaata
//
LOCUS       DACEPW010000064        13146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEPW010000064 DACEPW010000000
VERSION     DACEPW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.065"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..156)
                     /locus_tag="IW819_001451"
     CDS             complement(<1..156)
                     /locus_tag="IW819_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1244562.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPS"
     gene            complement(168..656)
                     /locus_tag="IW819_001452"
     CDS             complement(168..656)
                     /locus_tag="IW819_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1244563.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(733..3429)
                     /locus_tag="IW819_001453"
     CDS             complement(733..3429)
                     /locus_tag="IW819_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1244564.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(3857..4930)
                     /locus_tag="IW819_001454"
     CDS             complement(3857..4930)
                     /locus_tag="IW819_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1244565.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(4927..5754)
                     /locus_tag="IW819_001455"
     CDS             complement(4927..5754)
                     /locus_tag="IW819_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1244566.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(5755..6561)
                     /locus_tag="IW819_001456"
     CDS             complement(5755..6561)
                     /locus_tag="IW819_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1244567.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(6573..7658)
                     /locus_tag="IW819_001457"
     CDS             complement(6573..7658)
                     /locus_tag="IW819_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244568.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(7675..8217)
                     /locus_tag="IW819_001458"
     CDS             complement(7675..8217)
                     /locus_tag="IW819_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1244569.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8475..9464
                     /locus_tag="IW819_001459"
     CDS             8475..9464
                     /locus_tag="IW819_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1244570.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            9461..10198
                     /locus_tag="IW819_001460"
     CDS             9461..10198
                     /locus_tag="IW819_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1244571.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            10195..10746
                     /gene="coaC"
                     /locus_tag="IW819_001461"
     CDS             10195..10746
                     /gene="coaC"
                     /locus_tag="IW819_001461"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1244572.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            10762..11394
                     /locus_tag="IW819_001462"
     CDS             10762..11394
                     /locus_tag="IW819_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1244573.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            11479..12126
                     /locus_tag="IW819_001463"
     CDS             11479..12126
                     /locus_tag="IW819_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1244574.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            12131..13114
                     /locus_tag="IW819_001464"
     CDS             12131..13114
                     /locus_tag="IW819_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1244575.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         4042 a         2716 c         2314 g         4074 t
ORIGIN      
        1 cgatggtgca gccatcttca atgtcatctt ttgaatatcg ttggtagccg cctcatcgct
       61 tgctactact aacccggtta aatttagact ctttgtccaa gtcatcgcta cttgtccatg
      121 aattaatcgt tcatcaattc gcactgcttt aatcattttt cttcctccta aaaaaaatct
      181 tcctcttcat ttactacatg tacctcatca agaatggtca tctttaattc ctgttggcat
      241 ttatttacca gttcctgaat atcctccttt gttaattcct tggttgccaa tactatttcg
      301 agcagaattg gcagattaaa gccagacaaa atagttacag caatctcttt ctccaaaaag
      361 gttttggtaa tcttctgatt gacacttccg ccaaataaat cggtaaaaac aaaaatctga
      421 tcctgctcgt tcacctggtt tacaaataca ttcaactcct gttcaaaatc acactcatcc
      481 acataagcat taatcaccgt aatctcttga accttccccg tcagtaattg aatactgcta
      541 agaaagcctg aagctaagtg accgtgagtc gctattagaa ttttcttcat atggttaatt
      601 cctttctttc tgttcacttt tatataagca attcctgtgc caacttttaa aaacattggt
      661 ttaatcacgt taattcacat taaaaaaagt gtctaaccta aaaaaaacag attagacact
      721 tttttagaca ctttattttt gaatatacgg tcctaataat tcatagagca gcgaaagctc
      781 ataagcattg actttaaaat tatatttcat ctccattgat tgaaaaatac ttctagaaac
      841 ataataaaat tcatcttcat ctggtttcaa atcataattc ggttcttcag atttttctgt
      901 ccgagccaag aataaacgtt ccatcatcaa tgccgtatgc atatagagat ttagttttac
      961 acggccattt aaagtgaaat gatagtaatt ttcatattta gaaatgactg tttccacttc
     1021 actcatcacg atcgttgggt tcagaaagtt caaacggtcc gcaattcctt ccaacgaaaa
     1081 gaattttact agctcctgaa tcaagcggtt aaattctgcc ggcttcattt caggactcaa
     1141 aatatgctcc aaattggctg ctccatcacc gtctaagatg tcataaacat taatattagg
     1201 aattgaaaag gaacgtggaa gatccgaagt cgtgatgacc aattttgtct tgtcaaaata
     1261 agtttcatca ttttggtcga ttaattcacg tagttctctg tagtccttcg tgaaaacttc
     1321 gatggattca tccgataaat gatgacgaaa aacatctttc aatttgtccg aaatccccaa
     1381 acccgacata caggaaatga taatgttgct actttgagaa aagccttcaa agtattgaac
     1441 attgattgaa tacttgtctg ctgcatcctc cgcaatctgt ttaaatggcg cattcatcgt
     1501 catcttcaag ccaatatcaa gtgcaatgga agtcgtcaaa ttattcagaa gaagtaactc
     1561 cccttttaaa tcgttcttga tctgtgtgta aatttgattt aaagagccca tatctacgat
     1621 cagcactaga ctatctgtat tcacttgccg tttcacgaaa ttccttgtcg attcgacaat
     1681 ctctgacaca ttactctcta ctggcatatc caacgcttca aaaacataat tgccgcataa
     1741 ttgattcact actgcttgaa tgctgctagc cgtactatct ccgtgagcaa gcaacaggcc
     1801 cttcagctcg atggattcat ctacataatc agataaagtc acgacaataa gcagtcttag
     1861 aaagttctcg tccttttctg agagcggtgg taaatgctcc aagaactgat tacataagta
     1921 agtcgtccga ggtagatttg cagctaatgt acgatttatc ccggttaagt ctgtcttata
     1981 gcttggggaa atgacatagg atctgctaat aatttctgca acaggtccac tcgtcgtcaa
     2041 tccgtacgtt tttcctaaaa cggataccca gacctttcta aacatctgaa tcatcaattt
     2101 tctactcatt gataaaaaag aatccttatc aatgtgatgc accaaatgct gtacttcatt
     2161 ctttatcgaa gaaaaatttt cattgaaatt accttcattt atcagctgag taattttttc
     2221 caaaatcgaa tcataatcaa acggtttgat tcgatccgtt tttatctttt gatttttctt
     2281 tatcaataaa ctagcaatcg gcaagctttc tacttcggat tccagcatat ttaactgaat
     2341 caagatctca tttttcccaa tcgttaatgg ttcttcttgt gttcgactat acacatccgc
     2401 acaactgatt ttaatgatat tcttcagctt accaatattc ccactaaagg gatgagataa
     2461 cattaaagac aaggcttctc ttgaaacaat aatatctttt tgtaaaacat ccgcttcatt
     2521 ttgaaaaaat aattgaatca gctctaatcg ttctcgaatg gggcgatctt caaaagttgg
     2581 caacataaca ctaatttgaa tacgccgatc aaaggtgtct aataaataat tttcaccttt
     2641 ttccgtcgtg gcgaaaataa aacgaacctt agcagaatgc caatttacat tttcaccgac
     2701 tggacgatag tttccagtat caataaatag aaagagcttt tcttgatttt ctttcgatag
     2761 gcgatggacc tcatcgagaa aaagatagcc gttgttggcc gttgccaata aaccatcggt
     2821 atccttttca gcccccgtaa aggccccctt cttgtatcca aacagtgttg ccgaaagcag
     2881 ttcgggattg tccgcataat cggcacagtt taagatagca aatggtgcat ctttttcaat
     2941 aatttgctca tggatagcgt attcaaaaat ttttgtggct aaaaaacttt ttccaacacc
     3001 cgtggctcca tttattaata cactgagtcc gttaggcgga tatttgacag cggctataca
     3061 tttttgaatg acctctctca gacttccgtc atgaccaact acagaatgaa aggcttcgtt
     3121 tatttgctcc gtattatcta caaccgcata ttcatctaaa ggcttccaca aaacaggctt
     3181 tcccggtaat ttctcgacct ttttatcttc cactaatctc gtcaaataat ggctggcatt
     3241 ctgacgagag atacctagat tcttcgaaat tttctcggca gtgacagaag acatattccc
     3301 tgttagtcgt atcagctcat caaaaactcg atctttagcg ctctttttca ttttttcctc
     3361 cagtttaatg tgtctaaaag cataataaac tatcggctgc aatatgtcta ttctatattt
     3421 gtattccaat ttttactttg aaacacaaat agtcgaatat ttgcctcaga tatctgacaa
     3481 aataatttat ctaccaattt agtttttatc taatatgatt tttatgtttt ttcatagcta
     3541 caaatattga actggattca tatattagtt ttattaatag attacagaag ggttaagtat
     3601 atttgcatag tttccaatta ctttatcctt ctgtaacttt ttcaatctta gatttactag
     3661 cccgtctcgg gccctatttt acaatggttt tgtccacttt tctctggtag aaaaacgtgg
     3721 tagaaaaaat aaaataacac aaaaaaagcg gcgtttcatc cgcttttggt agaagattgg
     3781 aaaactcaga tagaaaaagg acgattttta gctctttaga acgttgataa gtccccattc
     3841 ttactgagtt ttgccattat ctcctataca tcttgaattc taagaatagg tcattataga
     3901 tgcctaggta tttcgatcct agatctttgt agacttcaag atgggagatc gtgtcatcag
     3961 atggatagaa ttgttcgtcc gacgaaatgt cttttggcaa taacgctttt gcttcttcat
     4021 ttggtgtaga ataaccaata tactctgcat tttgtgccgc attttccggt tctagcataa
     4081 agttgatgaa atcataggca ccttctttat ttttcacggt ttttggcata acgatattat
     4141 caaaccaaag attggatcct tctggtggga tcacataatg taaatgttca ttttcccaca
     4201 tcatatcggc agcttcgcca gaaaatgtca ctgccacgga ggcttcttca ttgatcatat
     4261 acattttgat ctcatctgcc acgattgctt tgacatttgg tgttaaatga ttcaatttat
     4321 ttgctgcttc tgtcagctgt gccatattct tactattcaa agaatagcct aaactattca
     4381 aagaaagccc cataacttct cgtgcaccgt caatcagcat caaactgtct tttaattccg
     4441 gcttccacaa atcatcccaa tgcttaattt gatttgcatc aaagaattta tcgttatata
     4501 cgatccctaa agttccccaa aaatatggaa tcgaatactg attatctgga tcaaaatcca
     4561 agtctaaaaa tcgtggatca atattattta atcctttgat cttcgaatga tctaatggaa
     4621 gtactagttt ttctttgatc attttttgga tcatatattc acttggtata gcgatgtcgt
     4681 agtttgtccc accttgctgg atttttgtga acatcgcttc attagaatca aatgtttcat
     4741 aattcacttt gtatccatat tctttttcaa atttcgtgat caaactagga tcgatataat
     4801 ctccccagtt atagatggta agtacctttg ctccactcat gccacttgtt ttttcaagtt
     4861 gacgaacacc aagcagcagc accgcaataa tgatgagaat acctaaaaac agagattgta
     4921 attttttcat cgcagccgtg ccaccccctc gcgtactctt ctgtttttct tcagccgttt
     4981 tggacggttt tctttactaa taaagtagta tccaatgacc aatatcatgg agaacaagaa
     5041 aactaacgca cttagcgcat tgatttctaa actgattcct tgtcttgcac gagaatagat
     5101 ctctactgag agcgtcgtaa agccatttcc tgtgacgaaa aatgtaaccg caaaatcatc
     5161 aagagaatag gtaaatgcca taaaatatcc agctatgatt cctggagaaa gataaggcaa
     5221 aatgatattt ttgactactt ggaaattatt agcaccaagg tctctagccg catcgaccat
     5281 tgaatcgttc atttcctgca attttggcaa gaccatcaag acgacgattg gaatgctgaa
     5341 agcaatatgg gaaagcaata cactctgaaa acctaatccg aatcctagta gtgtaaagaa
     5401 aatcaagaaa ctagcaccga taataacatc aggagagact aataagatgt tattcatgct
     5461 caacaacgct gtccgtgcac gtcgtttttt cgtgtaatag attcccatag ccccaaatgt
     5521 cccgatgatc gtggcgatca atgcagataa gaaagccagc atgaacgtat tcaatacgat
     5581 aatgatcaaa cgtgtatctt caaaaacagc ttcataattt tcccaagtaa atcctgtaaa
     5641 gctgttcatc gttcctcctt cattgaagga ataaaaaatc agatagaaga tcggtgcata
     5701 taagaataaa aagacgatcc ctaaataaag atacgaccat ttaaattttt tcatttatga
     5761 gcgacctcct tttttcttct cgcctgtcag gagcatcacg atgaacatag caatgattaa
     5821 aatcacaccg attgtagaac ccatccccca gttttgtgta accaagaaat gctgttcgat
     5881 agccgttcct aaagtgatca cccgatttcc gccaatcaga cgagtcagca tgaataaaga
     5941 aagagaagga ataaatactg cttggacacc acttttcaca ccatttaatg ataacggaaa
     6001 aatcactcgt cggaaggtct caaagctatt cgcgcctaaa tctctactag cactgatcaa
     6061 agaaggattc atctcttcca atgcattgaa aattggcata atcataaatg gaatctcgat
     6121 atacgttgcc acgaacagaa aactgaagtc tgtgaataat atctgtttcg gcccgatccc
     6181 gataaaatca aagaaatgat tcacgctgcc gtgcacactg aaaatcccaa taaatgcgta
     6241 agctttaagc agcagattca cccatgtagg caaaataatc agcatcagcc ataattgctt
     6301 gtgcttcagt tttgttaaaa aataagcagt cgggtaactg atgatcagcg tgaaaaacgt
     6361 gatcaaaaat gcataccata cagaattgat cgtcatagaa agcgatgtcc ccgacgtaaa
     6421 ataagataga taattattca gcgtgaattg cccattcata tcaaaaaaag attgataaat
     6481 gatcattaat acaggcgcga tcacgaataa accgagccac aaaatataag ggacggaata
     6541 gatcctgcgc attgttttca ttcttgtccc ccctattctt cgtagctgtc aagacgtgca
     6601 tcgaattctt cttcagactc gttgaaacgc atgacatgga tatcttctgg ctcaaaggcc
     6661 aatcctactt tgcttccttc tttagctttt ttcgttgaat ggaccatcca ttcattttgc
     6721 tgttcatcat aacagatgat ttcataatgg acaccacgaa acagctgagt atcgaccgtt
     6781 acgactagtt ttcctttatc tggtgtcgtg atcgtcaaat cctctgggcg taaaacaact
     6841 tcaactggtt catttagacg catcccgcca tcgacacatt caaattgctt accaacaaac
     6901 tcaactagat tatcttcgat cattacaccg tccacgatat tgctctcccc aacaaaatca
     6961 gcaacgaaat gattgattgg ctcatcataa atatctacgg gtgtaccact ttggacgatt
     7021 ttccctttat tcatgacaaa aatctcatca ctcattgcca acgcttcttc ttggtcatga
     7081 gtaacaaaaa taaacgtgat tcctaatcgt tgttgcagat cccgcagttc atactgcata
     7141 tctgtacgta gtttcaaatc aagcgccgat aacggttcat ctaatagcaa tactttcggt
     7201 tcatttacaa ttgctcgtgc aatcgctaca cgctgtcttt gaccaccaga catttcacta
     7261 atctcccgtt tttcgtatcc aggaagctgg accatacgca aagcatcttt taccttcttt
     7321 tcgatatctg atttggacat tttcttgatc ttcaatccaa aagcaacgtt atcaaaaaca
     7381 ttcatatgag gaaaaagtgc ataatcttgg aagactgtat ttacttgtcg cttattagct
     7441 ggcaaatcat tcaaccgttt cccttcaaaa tagatgtccc cttctgtagc ttcggtgaat
     7501 ccagctataa tacgtaggat cgttgtttta ccacagccgg aaggaccaag cagtgtataa
     7561 aattttcctt gttcgatctc aaaacttact ttttttaata ctgtttcatc atcatagcgt
     7621 ttcactactt gatcaaatga aatgattggt ttccccacgt ttttaccccc tatattacaa
     7681 atatgattgc gttgcaacaa tcaatacttt tgttcgtttt ttgagatgat tccttaattg
     7741 atgttgtttt gtcgcttgat aatagatcga ctgtcctgct tttgccacat agaccgtctc
     7801 tcctaagctt aactctaatt ccccttctaa tacaaaaata aacgtttcag ataatgaagg
     7861 ttcaaacgtc ttatattctc ctgcaccttc aaaagtcagc aagacaggct ccatttcttt
     7921 ttcattcgaa tcagccacga gccattccaa ttcatacccg ttttcttcat cgaaataaac
     7981 cgtatgatct tcttttgaat aaaccacttg cagattcgct gcttcttgat gaaaaaattc
     8041 ttctggagtc acacctaaga cttcaagaat agaaaagaat gtttccattg atggtgaact
     8101 taaatctcgt tccaactggg aaatataccc ttttgacaaa tctgtgcgtt cgcctagttc
     8161 ttcttgtgtc aaattctttt ggatgcgcaa atttcttaac ttctcaccaa tttccataat
     8221 ttcctccgaa tcgaagtttt ctaatagtaa acttcctgtt tactaccact cgtattagta
     8281 tacgaaattt attgaaaaag gcaacccttt gtaaaatttt ttttgctgaa tatttcttta
     8341 tcttttggaa taaattctag ttgtttgatt ctttattttt cacctaattt ttgtctaaat
     8401 ttacggaaga acaagaataa aagagtaggc tttatggtat gataaaagaa aattttaaaa
     8461 ggagtgtctc acgaatgaac gcttttcgat cttttcaggt aataaaggaa cctgttttcc
     8521 atacagcaat cagtgatctg ccatttactg agttggctga aaatgaagtc ttggtaaaaa
     8581 ttgcctattc tgatgtcaat tataaagatg cattggcaat gagcgagtct ggtcaggtga
     8641 tacgaacata tccgatgact ccagggattg atttatccgg aactgtcgtc caaagtaaca
     8701 atccacgttt ttcaaaagaa gatcttgtac ttgctactgg ttttgggctt ggcgtcaccc
     8761 atccaggcgg ttatagccaa tatcaaaaag taccaggaga ctggttagtt cctctaccaa
     8821 aaaatatgac gctgcgtcaa gcgatgatcc ttggtacagc agggtttact gctatgttgt
     8881 gtgtgaacgc tttaatccgt caaggtatga cctccgataa aaaagttgtc gttactggtg
     8941 cttctggcgg tgtaggcagt acagcgatag ccctgttaca taaactagga ttccagtcaa
     9001 tcattgcctt ttccagaaaa gaagagtctg tcacatggct aaaaagtcta ggtgctttgc
     9061 aagtcgtacg acctgatgaa tttctgccag agacaacaaa agctttagga aaacagcaaa
     9121 tcgactatgt catcgacaca gtaggtggcg agcagctaag cagtcttctt ccattgatct
     9181 cttataacgg tgcagtagct ttatgtggca atgctggggg aatcaaacta aacgcgactg
     9241 tcttgccttt tattctgcga aacatccagt tgattggaat tgattctgtc aatgtaccaa
     9301 tagatcaacg tctttcttta tggcaacaga tggcggattt gcagattgca gatgaactgg
     9361 tcgtccaaga gattactctg gatcaattgc ctgaaacagc atctaaacta cttgctggaa
     9421 cacaccaagg aagaacacta gtaaatgtag gtgaccataa atgaagattc taatcacagc
     9481 tggaggaacg actgaaaaaa tcgatcaagt acgtgctatt acaaatcatt cgacaggacg
     9541 attagggcaa gcattagctg attatttagc tgccaatccg gataccacaa tcgattacgt
     9601 gactacgaaa caagcactga aaccgaaaag acactccaat ataacgattt atacgatcga
     9661 atctgcactg gacttatttt tacaattaga agaactaacg aaaaaagaac actacgatgc
     9721 gattatccac agtatggcag tcagtgattt tactcctgct tttagttttt cagaagaaca
     9781 gctggcaaaa aacctaccca cttcttcaac gcaagaagaa ctggacaatt ggtttgcgga
     9841 aaatgaacag acagatacca ctgtttcaaa gatctcttct aacaccgagc atcttgtgct
     9901 cgttttgaag aaaacaccaa aaatcatttc ttatctacgt gaatggcagc caaaagcaaa
     9961 aatcattggt ttcaaactat tagtagatgt accaaaggaa tctcttctgg cagtcgcaaa
    10021 aaatagtctg ataaataaca aaactgattt tattttcgca aatgatttga cggaaatcca
    10081 tggcgagaca caccacggct atctcttatc taaagatgga acagtggaag aagctcaaag
    10141 taaatctgag attgctgcat tgattactga gaaaattaga ctggaggaat caaaatgaaa
    10201 aagatcttac taggcgttac tggaagtatt tctgcttaca aaagcgctga tcttacaagt
    10261 caattcgtca aattaggtgc ccaggtagat gtgattatgt ctaaaagcag cacacatttt
    10321 atcacgccat taaccttgca atctctgtcg aagcgcgcag tccatacaga cgtcatgcaa
    10381 gaatcaaatc cgcaggtaat caatcatatc gaactagcaa aacaagctga cttgtttctc
    10441 atcgcgcctg ctacagcaaa cattatcggc aagctagcaa atggcattgc agacgatctt
    10501 ctttcaacag tcgcaatggc gcttccagtg ggaacaccaa aattgattgc ccctgccatg
    10561 aacacgaata tgtatcaaaa cccgatcact cagcgtaatc tggaaacatt gagatctgtc
    10621 ggctatcagg aaattgaacc aagagaatct cttttagctt gtggagattt tggaaaaggt
    10681 gccttagctg atattcagac gatcatcgaa caggtcatgc tgactcttaa tgaaagaaat
    10741 gcttagaaaa aaggagtact tatgaaaaat acaaaaaact ttacattaac tgcgatgttt
    10801 ttagctattt tgattttatt agctgttacg cctttaggtt ttatccctat cggtccaatc
    10861 aatgcaacaa ccatgcatat tcctgtaatc attgcttcga tcgttttagg accaagacta
    10921 ggtgcttttc ttggcggtac atttggactg atcagcatga tccgtagcac agttatccaa
    10981 acacctttgt cttttgtctt ttctccgttt atcccagtca tcggaacaga acatggaagc
    11041 tggaaagcct tgctgatcgc gtttattcct cgcatcttga ttggggtggt tccttatttc
    11101 gtttataaag gtctgcgcaa actaatgaaa caaaaagcag attccgtttc tttattctta
    11161 gctggacttt ccggttcatt gaccaatacg attttagtga tgaatatgat ctacttttta
    11221 ttccagcaag attatgccaa agtcatcgga acaaatttaa atgctgttta ttcagctatt
    11281 ctagcggtta tttttacaag tggtatacct gaaggaatcg tcgcaggtct tgcgactgca
    11341 gcagtatgca atgtcttgtt aagatacttc aagcaccctt ctcttactaa ctgatagtca
    11401 tcgtagccga tctctgattt ttttctcttt tggtgttact attgaatgga aagatttgac
    11461 gtcgaaagga tgatccaaat gaaaattttt gttgtaggtg caaacggaca aattggccga
    11521 cacctgataa aagatttagc tccaagttcc catgaaattt ttgcaggggt gagagatgtt
    11581 gcttcccaaa cattagtaaa aaaagagaat gtttcgtatg tctcttttga tttaacttgg
    11641 tccgtggaaa aaatgaccga agcattcaaa ggcatcgatg tattgatttt tgctgccggc
    11701 tctcaaggaa agaatcttct gcaagtagac ttggatggcg caatcaaaac agtgatcgca
    11761 gctgaaaatg cgcatgtctc tcgctatctg atggtcagtg ctgtttatgc agatgaacca
    11821 gcaaaatggc ctgagtccat gacagattat tatattacta aacattatgc agatgaatgg
    11881 ctgaaacgaa ctaacttaga ttttgtcatt cttcaaccag tcacattgac taacgatgaa
    11941 gaagttactt ccattcaatt aactaagcct aatgagaagg catcaaaaac gatcactcgc
    12001 tctacagttg ctgctgtact tgccgctcta gtggaagaaa cggatatttc tcgtacgact
    12061 ctagtacttt ctgaaggtag caaagagttg aatacagcat tccaagaatg ggcaaaggag
    12121 gaatagatcc atgcgtgcca tttcaatcaa gcatcctggt tctgtagatg tattagaaca
    12181 aattgaagtg cctgttccta aaccaaaaca aggcgaatta ctgatcaaag tccatacagc
    12241 agctgtcaat cgaacagata ttatgaaaag agaatcagtc tctttgaaac ctccctaccc
    12301 tattttggga gtcgaagtag ctggagaggt agttgagaat aaaagtgata tctccacttt
    12361 tactcctggg acaaaagtat acggattagt caatctaggt ggttatgccg aatatgctgt
    12421 gatgcctgcc gatcgtgcaa tccttctccc tgatacgcta gactatgttt ctgctgctgg
    12481 aatctcagaa gtttttttga ctgcttatca aacactttac tggcttggaa aattacaaga
    12541 aggcgaaact gtcttgatcc atgcaggagc aagtggtgtg ggaacagcag cgatccaatt
    12601 agctagacag ctttctcacg cgaaaatcat cgttactgca ggatcagaag aaaaactcgc
    12661 cttttgtaaa gaacttggtg cagatgaatt gatcaactat aaaaaacaag actttcaaga
    12721 agaggtacga aaaatcactg aaggtcgggg agtcgatgtt attttggatt tcatcggcgc
    12781 ttcttactgg gaaaagaatt tagcttctat agccgttgat ggacgttggg tcttgattgg
    12841 tatgctaggt gggaccatcg tgcctgagat agaccttggt gtattgattg ggaaacgtgt
    12901 ccaactgctt ggtacattat tgacacctag aagtgatcag tataaagcag agctcgcaca
    12961 aacttttatg caaaaagctg ggccttttct agaaaatggc aagattcggc caatcattga
    13021 ccgtacattc caattcagcg cagtcaaaca agcgcatgaa tatatggaag caaacaaaaa
    13081 catcggaaaa atcattttga agataactga ataaaatcat gctgatcaaa aaagaggctg
    13141 ggacag
//
LOCUS       DACEPW010000065        13098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEPW010000065 DACEPW010000000
VERSION     DACEPW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.066"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(212..646)
                     /locus_tag="IW819_001465"
     CDS             complement(212..646)
                     /locus_tag="IW819_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1244576.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(878..1588)
                     /locus_tag="IW819_001466"
     CDS             complement(878..1588)
                     /locus_tag="IW819_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1244577.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1578..2438)
                     /locus_tag="IW819_001467"
     CDS             complement(1578..2438)
                     /locus_tag="IW819_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1244578.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2443..3081)
                     /locus_tag="IW819_001468"
     CDS             complement(2443..3081)
                     /locus_tag="IW819_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1244579.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3096..3395)
                     /locus_tag="IW819_001469"
     CDS             complement(3096..3395)
                     /locus_tag="IW819_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1244580.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3438..4922)
                     /locus_tag="IW819_001470"
     CDS             complement(3438..4922)
                     /locus_tag="IW819_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1244581.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(4942..5403)
                     /locus_tag="IW819_001471"
     CDS             complement(4942..5403)
                     /locus_tag="IW819_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1244582.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5421..6488)
                     /gene="ulaG"
                     /locus_tag="IW819_001472"
     CDS             complement(5421..6488)
                     /gene="ulaG"
                     /locus_tag="IW819_001472"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1244583.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6706..7476
                     /locus_tag="IW819_001473"
     CDS             6706..7476
                     /locus_tag="IW819_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1244584.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7749..8576)
                     /locus_tag="IW819_001474"
     CDS             complement(7749..8576)
                     /locus_tag="IW819_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1244585.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9144..10379
                     /locus_tag="IW819_001475"
     CDS             9144..10379
                     /locus_tag="IW819_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1244586.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11378..11638)
                     /locus_tag="IW819_001476"
     CDS             complement(11378..11638)
                     /locus_tag="IW819_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1244587.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11665..12966)
                     /locus_tag="IW819_001477"
     CDS             complement(11665..12966)
                     /locus_tag="IW819_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1244588.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4146 a         2735 c         2049 g         4168 t
ORIGIN      
        1 aaaaaattat cgcatagcat ttgctttaaa aaaaaatcat gtgttcattt tttatattag
       61 ttcgaatttg caaaaaattg tatttgataa agaggtgagt aaaaaaataa aatagctgag
      121 cattggagga tagattgagt gatactaagt ctatcctctt gtttttctaa agaactaagc
      181 tagaattttt tcttttgtac gttattatat gttatttgtc attaccaaaa agcacctttt
      241 ttcttctagt atctatgaca taagaaatac cacccatttc ctttacttga acaaatccta
      301 ctgattcata aaattttttt gttttctcag tatcatctgt caataattcg atttggactg
      361 gtccataact agtgatcttc tccaaagctt cattcaaaag cactttgcct attccttggc
      421 gctgatacct cggatgaatg aggatatctt gaataaatgc gatcgtcgca aaatcagtga
      481 tccaacgaac gattccgata atcttccctt ccccattacg ctttatcaga gattcagatt
      541 gttcaaatgc tctagctaac cttgcagtgt cttttgtgta attcgtccaa ccaacgctct
      601 tatataattc agaaatctca tcgatcataa gggaatcaat attcattttt ttcttccttt
      661 cttatacaat tctccctcta ataatacagt tctttgacta tttaatctat cacatataac
      721 tctcctgtaa agattctctc gattccttaa aattcctctt tccttttaga atgcatactt
      781 tttgataaat aaaaaggttg tgataaattt tcttcacaac ctctgtttct ctctatcaag
      841 tgttatcttt taattttcta cccttaccct agcatctcta ttgcccgtag tatgcatact
      901 ttccatgttt gcgaaaataa tgtttatcta gtaaatattg cgggatttca catatattgg
      961 ggttgatcag ctcgttttct ttggccatca gacaaatttc atctaagatc aaactgtttt
     1021 ctactgcttt cataggtgaa tccccccatg tgaacggtcc atgtccatag acaagtactc
     1081 cagggacttc gtttggatct aattttcttt ccttaaacgt ctcgacaatc acattccctg
     1141 tatgaacttc gtaagcttct cttacttctt ctttcgttaa ttggcgtgta caaggaacct
     1201 taccataaaa agcatcagca tgagtcgtgc cataagctgg caaatcgcgt cctgcttgcg
     1261 cccacatgac ggagtgcgtg gaatgagtat gagtaatagc agttatatct tcaaaagttt
     1321 gatacaaaac aacatgtgtg gggagatctg aagatggttt taagctatct tcttctatta
     1381 tatttcccga caaatctgtt accaccattt gatctgcttg catgcattca tatctgacac
     1441 cgcttggctt aatgacgatg actcctagct cacggtccac ttcactcaca ttgccccaag
     1501 tcaatttgac taatccgtat cgcggcaatg ccagatttgc ttcaaggaca cgttgcttca
     1561 ttgctgcaat tttttcttca caagacatca tagccagcct ccttcaattt agaacttaag
     1621 tagtcttttg cctgctggat ttctcttcga aagtcttggc ttttttcact ccacatttca
     1681 atcagaaatg ttccatcata atctagacgt ttcaaatgtc ttaataatcc aagaaaatca
     1741 acacaaccgc taccaaaagg aacatcacga aactggcctg aatattcttt tgttacaggg
     1801 taagtatctt ttaagtggat agcagtgata taatcgatcc ctttctcaag ttctcgagcc
     1861 ggattatttt ccggccatgc ggacaaattg cctaggtctg gatatacttg tagaaaaggt
     1921 gaatgaatct gttctttgat ttctaaaaat tttgaaatgg agttcataaa tggatcatcc
     1981 atgatttcca cggaaagaat gatgccatat ttagaagctt cctttacgct ttcttttatt
     2041 ccttcgataa agtcttctct ggaattcatt gatttctctt cataataaac atcgtatcca
     2101 gctatttgga ttactttgac gcttagatta tgcgccaaat ggattgcttt tttcattagt
     2161 tgtttcgctg cttttttctt ttctggatca gcagaaccaa acggaaatcg ccgatgcgca
     2221 cttaaacaaa tcgaatggat acgtacaccg gagctgaaca tctcttctct cagctcagct
     2281 atcttttctt cagaccaatc taatcgtgct aaccgctcat ccgtctcatc aatcgacatc
     2341 tcgataaaat cgaagtccat ttcttttaca agggaaaaac gttctttcca tgaaatatct
     2401 ttgggtaacg ctttttcata aatcccaatt tgagccattt ccttaccccc agatacgaat
     2461 gatctcatct ttgaagtttt gtgcggcctt catcggatca tttgctgctg taataccacg
     2521 accagtgata aaggtataca catcgatccc ttcaaataag tgtaatgtgt gtggatttaa
     2581 tccaccagtt acagaaacat taaagcctag ttcaatcagt tttttgatct tcgataaatc
     2641 tttttcaccc caagtttcac cagataacag cgcatcacgg ctttgatggt agattacctg
     2701 ccggataccg atgttatgcc aatccatggc ttgttcaaaa gtccaattgc cgtagagttc
     2761 tacttgcaat tcacctattt ctttttgtgc tgcctccata gttggaatcg ttgcacaaca
     2821 aatgactgtc atccagtctg ctcctgcttt tgcaacatta ctcgccactg ttcctccagc
     2881 gtctgcacat ttcgtatcag cgactagttt tttatctggg aacatactgc gaatacagcg
     2941 aatcgctttt tgtccttcct gtaagcaaag aattgtgccc acttcaataa tatctactac
     3001 atcaccaact ttcataacat ctcctaatgc atgttctaag tcattgtgat ctaacgcaat
     3061 ttgcaaattc ggcctgctca tcttagttcc ttcctttact tgtttttttg gtaccacttt
     3121 gtttcagcaa atttttcact gatttctttg actgacatca catttttgat accgataact
     3181 gtaactcctt ttttctctgc ctgagtaaac atattcaaaa aattgactgg cgtaaaaact
     3241 acatcgtatt ggccagctga actttttccc tctgaaatgg cacaatgatg gattttggta
     3301 attggtacgt ttagttcttt cattgctttt tccacacttc tcatcatcat caagcttgtt
     3361 cctgatccat ttgcacacga tactaaaatt ctcatcttaa tccctccatt atgagtgttg
     3421 tctaatcaga ttttctttta tactctctgc ttttctgcat atgtgtcata gtcttctaca
     3481 atcaaaaaat aaccatcagg atttttccga tattgtaact gcggaatagc caataaacca
     3541 atgacaacta cggcaattcc agcataacct aggactttca tgattactgt aaacaatggc
     3601 caagctgttg cccagtcgaa catcccgata taaccaccgt atgttgacat cccaatccat
     3661 gtagcgaata atgctgaacc gcctacttgt atgattcctg aaagaaacgg gaacaggcaa
     3721 gcggctttga atccacctcg gttattggca taaactgcta taacggcatt atcaaagaac
     3781 aacggaatga aaccggctat gacgatcgtt ggtgatttca ataaaatcag taaaccaatc
     3841 atcaaaaact gtcccaaagc accaaacaag aacccaatcg ttaccgcatt tggcgaacca
     3901 aagccaaaag tagcagctac gtcaattcct ggaacagctc ctggtaaaat cgtgtttgag
     3961 attccttgga aagattgagt tagttcatcg acgaatgtcc gaacacctaa ttgcaagatc
     4021 gccagatata ccgcaaaatt taaagaagtc gtcataatgt agaataagaa actctgccct
     4081 tcttgcatga attgcgcttg aatcaaataa tctttgccta gtacaatcag aatgatccca
     4141 aagaaaaata gcatgagtaa cgaagtagca accatattct cattgaaaat cgacaagaat
     4201 cctggtaatt tgatatcttc aatttttttg tcttgcttga ttgatctatt tttcttttca
     4261 tcccgctttt tcatccactc agctaatttt gcaaatataa agattccgaa catttgctga
     4321 tgtgccactg caaatccggc accttctgtc aattcctgcg tgattcctac tgttaagtta
     4381 gaagctactg tccaataaca acctaaaatc agtcccataa aaatcaatgc ttgtatccta
     4441 cctagatcag gaaagcaaaa cagtaaaatc caaaaagcgg tagctgcttg ttgaacctga
     4501 acgtttccgg tagtgaaaac ggaacgcagt ttcgtatatt tctgaaaacg gactaataag
     4561 atatttaaaa caaatgcaat cagtaataaa atcatcacat ctccaaaggt acggccaaat
     4621 gtctccatta atccagcatc cacagcattt tgtccaaaat aaggatctgt aaccatggca
     4681 tccaaattga accgttcctt tagtccaacc aagattggcc gaaaattatt gaccaaacca
     4741 ccagagccaa cagtcaaaat aaagtaaccg accgttgcct ttaagaatcc agcgatgcat
     4801 tcatgaagcg ggcgtttcag caaaatatag cctaataaca cgataaaacc aatcaggtat
     4861 gctggctgcg tcaagatatt tgttgcaaaa tacgtccaaa tgcttaacaa aaattccccc
     4921 atcttttttc tcctctatca attaattata ttctgccatc acaacttggt aatcatcgat
     4981 ggagttcgta gagatgagac gctcaaccaa tccatctgtc attaataatt cggataattt
     5041 ttggatattt tctagatgtt cttctggatc ttttgctgcg agtgtaaaaa acaaactagc
     5101 cgttttctcc tcattctccc cttcactttc aaaacggatt ggctgtttca acttggtaaa
     5161 tgaaatagct gttccaaaaa caccatcact tttttctgaa gaatgcggca ttgctacttg
     5221 aggtgcgatc acaatataag gaccgtattt ttctacacaa tggatgatct cctccacata
     5281 cgtttcatca ataatttcct tatcgatcaa tacaagacag ctttcggcga tagcttcacg
     5341 ccaatccgtg atttctttat ctgggtaatg aatcaattga tggtcaaaaa agtatttcag
     5401 caagaaactc cccttctttc ttataaaaac gacggaaacg gtgtatcatt ttcaatcgag
     5461 aagacatctt caaatcctcg ataaaaatta aactcaagat catctttgtt tgtcggatag
     5521 atgaatttcc cgcctacttg ccagatataa ggtttaaatc catacttcag gcgatccttt
     5581 ttgaatttcc aaatttcagt gatctctttt ggatcagcca taaaattaga ccaaatatcg
     5641 tagtggacag gaatcactac ttttgcattc aatgactccg ccatgcgcag catatcaaca
     5701 gaagtaactt tgtctgttat ccctcgtggg ttttcaccat aggcccctaa acagacatcg
     5761 attttgtgtt cattaccatg tttggcaaat aaattggagt aatgtgaatc tcctgcatga
     5821 taaagattcc ctcctgatgt ttcgaataaa tagtttactg caatttgatc catctcctga
     5881 ggcatttttc cttttaatac ctcttctgga tcttcacaag tgaccaacgc tgtccgatca
     5941 aacgcttcta aagctactac attgatgtct ttgattttga ctgcatcacc tggctttact
     6001 gtaatcgttt tttcttcagg aattccccat ttttgccacg tgcgaaccac ttcttttggc
     6061 ccaatgaatg ttgcttcagg acaattttga tgaactgctg cggctgtatg gatatctaga
     6121 tgatcggaat ggatatgtgt aacgattaaa gcgtctacat tttttatcgc aaaaggatcg
     6181 attacgaaag gctgagtacg taaattaggc tgcatatttt gaacgccact catacgcatc
     6241 atctgatggc cttttttcat catgccattc ccatgactac gttttcccgt cccacaccat
     6301 agatcacata aaatattcgt tccttcatga cttttcagcc aaattcccgt acagcccagc
     6361 caccacatag caaaagttcc aggatccact acttctgttt cgatttcttc attcaagtat
     6421 gtgccccact caggaaatgt ttctactaac cacttttctt ttgttacttc attgacagtt
     6481 ttactcattt gcttgctccc cccatatcgt tgttccaact tacaagtaaa tattaatgtg
     6541 tatcagcatc catgaaaacc cttttatatg accgtttgtt ttttatatga ttttctttca
     6601 gtaatcaatg attgtttcag tataataaag acagatatga atgtttaaat gaacaactag
     6661 tcaaataaat ccatggatca agatgtccct caggaggcaa aagtcatgaa aaactcactt
     6721 gtcaatattg aaaaacgtca aaaagaaatt ttggaactgc ttcagcagac tgaacaacta
     6781 tcaactttcg aattagctga atcccttgat gtatctatta gtacgatacg aagagattta
     6841 aatttattag aagaaaaaaa cgagatcata cgcaattacg gttattgttc ttataattac
     6901 aaaaaccaga ctgattttga tcaatctggt cctgaaagga ttaaacaagc gattgccaga
     6961 gaagctagta aatacattaa cgattatgat acgctgttta tcaattctag ctcgactgcg
     7021 ttgaaaacac tggattttct tcaatctaac catttgacag tcattacaaa caacgtgaaa
     7081 atcaattact ctccgcacaa agataattgt agttatattt taactggtgg agagatgcgc
     7141 tttccaaaag aagcactagt gggcgacatc gcattaaata cgatttcttc agtcaatgca
     7201 gacatttgta tcattggctg tagcggagtc gatgtagtac atggggtcac aacaaagata
     7261 ttaaatgaat caacgatcaa cgaaatgatg gtcaacaaga caatcaaatg taagatttta
     7321 gtcgctgatc atcgaaaaat cggactgaca tcaaagttca agattgctga tatttcagtc
     7381 tttgattatt tgatcactga taaattttgt tctgaaaaag tattagagga gatcagaaat
     7441 acggggataa aaaccattca agtagattca ttttaagaac caggacgtaa atatgtaaat
     7501 aggattatca aaaaaatgaa taacaaattt atcgataaat gaaaaacacg ctacgctcaa
     7561 attgataaag gaatcccaag atgtatccat ccaatcgata ccatcataat ctattgcttc
     7621 ttttataacc gttatatgtt gcctaaatag gaatcaaaca atatcgaaaa aaggaggtat
     7681 gacaaaagtc acacctcctt ttttcgaata aacggtattc aaatcagaac actgatttca
     7741 caaccacttt atttcaggtc gttttcaatc acctttaccg cgtaatcatt tccgttgaaa
     7801 cgcgctattt tttttgcgtc catatatctt tctaaataat catcttctcc taattgctta
     7861 tggcaagaag caatttcata aagcaagtat tccaatgcat aagtcgattt atgtttctta
     7921 ttatactcaa tacccttctc acataattgg attgctttat cataatcctc gattttacgg
     7981 taataggtgg cgatatcata atatacaatg cagaccatca gattatcaaa atcagtctca
     8041 ctcaatattt taagtgtacg ctccacataa actttcgcaa aatctaattc tccttttgac
     8101 aagtagatca ttgccacgtt tgcagctgct tgtaagtgat ggatatctgt ttcggtcgtt
     8161 tgttccaaca cttgattaaa ataaaataat gctgcatctg gttttttcgc ataaagatga
     8221 tcaagcagtc cttgataaaa taaaatttga tttaaaagat cagaattact tactttttcc
     8281 tgatcgatcg tttgtaaaat tttataagat tcttggtatt ttgatacaga attcaatgat
     8341 tgaatctcat ttattttttt cctgattttg tcttcttctg tttctactaa tatatcggag
     8401 atctcgacat tcagcttttt gcaaagcgca cttaagatat tgatgcctgg cgacatattc
     8461 ttgttttcga tattgctgac agttgcttgt tcacaaatgc cttctgctaa ttgtacttgt
     8521 gttaacccct ttttctttcg gtattctttt agtaatgctc catctacttt aatcaaaaca
     8581 atttccttcc ctttaaaaaa ttatttctcc tttatatcat acacaaaaaa aagttctaaa
     8641 caaccaaaaa tgtttagaac agaataaaaa ttttaatttt aaaataatat tcaaattaaa
     8701 caactggcgt atctgctcct tgatttcttt ttgtgttatc tgaataatta tcgtttacag
     8761 ttataacaca tagtgaaaat tgtcatggcg agaaagtcta agaatatttt taaacataac
     8821 tatttctttc ttacgtgtgt ataatgacga cagcacacct atcaattaat ataattattt
     8881 tattttttta tgttctcatg tttattttta atgacacaac ttctcgtatt tatctggcac
     8941 aacatataaa tcgatcaaaa aattgttgcg tgtttttttg aaaattggag tttcaacttg
     9001 attacaaaat ttaaatttag aagttgtgaa aacagcgaga gacagtttaa tattcaaaat
     9061 atacataggc tactcgaacg ctgtgttttt tagtatacta gtaagaaacg aagggataga
     9121 ctcattccaa taaggaggat ttcatgaaga caattctttt tgctcctgca gtattcaatt
     9181 tagctgagac aacacgaatg attgaggttg ccaaagctat gaaagaccgt tattactgtg
     9241 tattctttgg cttttctaaa acttttgctc ctttgatcac agaaagtggc tttgagtatc
     9301 atttactagc gccgatcctt acgaaaaaac aagaagaaca aatcataaaa tttgaccagt
     9361 tgcgtacact taaaaaccct tttaccaaag aaatggttgc ctctcgtatc actagtgaac
     9421 agagtctgat tgctaagttt gagcctcgac tgattgtgac aggttcgaat gtgacaattt
     9481 ttttatcggc ccgcaataaa aagattcctt tagtatatgt taaaccctat gctttgagtg
     9541 aacctttttt tcaatcagag catgcgctga tgccccacga gttacgtttc ttcggccctt
     9601 taagcaatcc cctttggcac tggacaaaaa agcgtatagt aaatacccga tggatcccca
     9661 atgccttcaa acacgctgca caaaaagaaa tgcttccttt accaaaaaca agtttacaaa
     9721 tgatggatgc tgatctaaat ctcatcacaa cgcctaaact atttttggaa gaagaacagc
     9781 tgccggaaaa ttatcgaatc gttggtccaa tttttgctaa gctcagtact tccttgcctg
     9841 aagaagtgct aacttttata cagcgaaagc aacaagaaag aaaacgtatt atctatttcg
     9901 caatgggtag ttcaggcaat ccgcgtatgt tacgcaaaat tttgttcttc ttacaaaaac
     9961 aaccggatct agcggttatt gcacctattg actatctgct tgatgacatt caatctgact
    10021 cagaagatct tttaataaca gaatatctgt ctagtcatct tttgcatgaa ttcattgatt
    10081 tttcttttat ccacggcggg gaagggaccg tccaaactgc ctgtgaatcc ggcaaaccgt
    10141 ttatcggtgt ggggatgcaa tttgagcaaa gctgtaatat tgactattgt gtaaaatacg
    10201 gaaacgcaat cgccctcaca aaaccagtga ctgaaagaaa actgcagatt gctctgcgcg
    10261 aagtttcttc acccaagatt cgtcagcagg cacgcacgct gcaagccgtt tttccaataa
    10321 atggtccaca acaagcaatt caagaaatcg aaaccttcct gagcaaccac ccaatatagt
    10381 ttcaagtaga aaaagctttt ttaatagaga ctcatcagta atttcgctat tcgagaccca
    10441 gacagtgaaa atacaaaaaa agtaaatagt ggattgaaaa gttagagtta cttaaattac
    10501 ggtgtctaaa aaacacaaaa tcatccttaa aataaaagat ctgacgctat ccaaatgagc
    10561 ataggtagcg tctgccattt attcttagtc aaattctttg acacctttct ctggttggat
    10621 catctcaacg tttcttcaaa agaaagacca taataattag gcaaatacat tttcttaaaa
    10681 tgtgtcatat agatctagaa gcatcgcttt gtagagattt gtgccgctag tcccctcaac
    10741 aattactaaa tcaaatgtag gatttaccac tttatctttg ataatcaacc tccctcttct
    10801 gttgagacaa tgatctcgca atccgatgca tactcctata taaactgcca gcaatcccca
    10861 catattgagt agatcatgtc agaatgttct atgtcgtcag caacgataca ctaaaagtca
    10921 cgttcaccat gactgattgc tattcccagc acaattattt ctatacacgt ttcatgattt
    10981 gaataaacac tcacatcata catattacca ttcatacacc atactgcagc ccctactata
    11041 tggttgtaag ctcccaaatc tatatttgac ttgatcactt tggtagtaga tctactaatt
    11101 ctaaatccat tgctgataaa atttccattt tttcctcctt ctttctgcca aaacagttaa
    11161 aacaataata atctttatgt aaaaaagggc tgtgacaaaa gccttgtcac agtcccatat
    11221 tccgaataaa cggtggtcaa aagcttgcat ctgcggtaat aagttcaacc aaacgaaaaa
    11281 taagccaaca tttcacaaaa atttaagaag caattttcgt aaatttcttc ttatttcttg
    11341 aagctaacca cttctgttac aaccactttt agaacattta aagcttcgat gtgacaaacc
    11401 atctatgctg ctcgatcata aaattgatag caaaaattcc acaaagtaca tatgtccccc
    11461 atgaaaacaa aacattcata tgcggtacaa tgatctttgg aagaatcacg atcaaaaatg
    11521 atgccagtgc ccaaatccat cgattgattt gaatatgata tgtagctagc atattcaata
    11581 taaggtgact agccgttaat aaaaagataa aaacaaaaaa taaaatcagc caatacattg
    11641 tttcccacct tctaaaaacc aaacttagac agtctggact tcgttttctc cacgactttg
    11701 ttcatctgac agcatctttt tatcataagc ttttacaaat ggataataga taataaatgc
    11761 tatcaatgtg ttgatcatga tcaagataac aacacgccag tcatttcctg ctgaaagata
    11821 accgccgatt ggagctggta atgtccatgt tggcaaaaca gtaaatctat tgaccatttc
    11881 taatttgact gcaaaataac tgataatagt caaaactaaa ggaactaagt tgaacgggat
    11941 gaacatgtaa ggattcaaca tcataggagc tccaaaaatt atgggctcat tgatattgaa
    12001 gataccagga ataatagaaa aacgaccaac ctctttcaag aattttgatt tacagaagaa
    12061 tataaacaaa acagatacca cgatcgttgc tcctgcacca ccaattgtta ccgtccattg
    12121 ataaaactgt tcaggagcga tatagggtaa ttgggtggca gctgtaccat ctgccaatgc
    12181 ctttgcattc tcatccaaca tgatcagcca cattggacga acaacagccc ctacaatact
    12241 catgccatgg ataccaaatg accacaacaa atggatcaaa aacattggta gtaaaacacc
    12301 aagcaaatta ttccctaaaa tattggttaa aggctcgaat aacgataata aggcagcgtt
    12361 tacatcaaag cccgcgacgc ttcgaatcag ccaagtaatt gttaaaatga ctgcacctgg
    12421 aatcaatgcc tcaaaactac gggcaacagc aggtggcact tgttctggca ttttgatagt
    12481 aatatttcga tttttaaaga aacgatatac ttccactgca aaaatcatag ataagatccc
    12541 cgcaaacatc ccagatgctc ccaaagaact cataggtaag acccagccaa gaggtgcctc
    12601 ttcaggagtt aaaacgttta caggaacttg taacataaag aacgttccta aagataatat
    12661 accacctgtc acactatcta atccatatga tttagctaga tccgaaccca taacaaaagt
    12721 tgcataaata gccatcaatc ccatcgtcat cctaaagggt aaaacaatcg ttgccgaata
    12781 aggggcaata aattcattcc atccaggaaa cggtaaattg ataaagacca taaaaaatgt
    12841 cccaatcaaa atcaatgaca atgttgaaac aaccccatta cgaattgcta ataaatgacg
    12901 ctgtgtacca atttttgcca ttggtgggac aagtttctct tcgacccatt tcatcagctt
    12961 ttccactaaa accactcctt ttcaataaaa tatgaaccat tcattgcatg acaacgcaaa
    13021 gaatgttcat aactaattat ataattaaga aattttattt caatatattc tcttaaataa
    13081 tcaaatttat ttttaaga
//
LOCUS       DACEPW010000066        13033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEPW010000066 DACEPW010000000
VERSION     DACEPW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.067"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..1202)
                     /gene="rpoD"
                     /locus_tag="IW819_001478"
     CDS             complement(93..1202)
                     /gene="rpoD"
                     /locus_tag="IW819_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1244589.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1226..3067)
                     /locus_tag="IW819_001479"
     CDS             complement(1226..3067)
                     /locus_tag="IW819_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1244590.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3249..5642)
                     /locus_tag="IW819_001480"
     CDS             complement(3249..5642)
                     /locus_tag="IW819_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1244591.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5749..6609)
                     /locus_tag="IW819_001481"
     CDS             complement(5749..6609)
                     /locus_tag="IW819_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1244592.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6668..7189)
                     /locus_tag="IW819_001482"
     CDS             complement(6668..7189)
                     /locus_tag="IW819_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1244593.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7509..8228
                     /locus_tag="IW819_001483"
     CDS             7509..8228
                     /locus_tag="IW819_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1244594.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8254..10845
                     /gene="mprF"
                     /locus_tag="IW819_001484"
     CDS             8254..10845
                     /gene="mprF"
                     /locus_tag="IW819_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1244595.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11033..12220)
                     /gene="tuf"
                     /locus_tag="IW819_001485"
     CDS             complement(11033..12220)
                     /gene="tuf"
                     /locus_tag="IW819_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1244596.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12588..12944
                     /locus_tag="IW819_001486"
     CDS             12588..12944
                     /locus_tag="IW819_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1244597.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3742 a         2725 c         2323 g         4243 t
ORIGIN      
        1 aaaaagcttg agactgcgta gtctcaagct ttttttcttt taaaataaac caaacagttt
       61 tactgatttc tcttctggtc aatagtccac gcttattcta agaaatcttt taattgtttt
      121 gaacgagaag gatggcgcaa ttttcttaat gctttagcct cgatctgacg aatacgttcc
      181 cgagtaacac caaatacttt ccctacttct tccaatgttc tcgtccggcc atcatctaaa
      241 ccaaaacgta agcgaaggac attctcttcc cgatcagtca atgtatctag tacatcttcc
      301 aattgttctt ttagtaactc ataagctgcg tgttctgctg gacttgttgc ttcctggtct
      361 tctatgaaat cacctaagtg agagtcatct tcttcaccga ttggcgtttc aagggaaaca
      421 ggttcttgcg cgattttcaa gatttctcgg actttttctg ttggaagatc catttccgca
      481 ccgatttctt ctggcgtagg ttctcttcca aggtcttgca gcaattgacg ttggatacgt
      541 atcaatttat taattgtttc aaccatatga actggaatac ggatcgtacg tgcttggtca
      601 gcgattgctc gagtgatggc ttgacgaatc caccatgtag cataggttga aaacttaaat
      661 cctttgcggt agtcaaattt ttcaactgcc ttcatcagtc ccatgtttcc ttcttggatc
      721 aagtcaagga actgcatgcc tcgtcctaca taacgtttag cgatacttac tactaaacga
      781 aggtttgctt ctgccagacg ctgtttcgct tcttggtcgc cttcttcgat tttcaatgct
      841 aacgctactt cttcttccgc agttaaaagg gagacacggc caatttcttt cagatacata
      901 cgaaccgggt cattgatttt tacaccggtt ggcgcagaaa gatcttctgt ttttgcttgt
      961 tcagcttttt tctcagcact cttcaagctg tgaattgatg gatcaccatt ttcatccacc
     1021 acactgatac ctgcgtcttc aatcttttgg atcaatttgt ccatttcatc tgcattcaaa
     1081 gtgaatggtg tagcaagctg attggataaa tcatcatata caacttgtcg tttgggtttg
     1141 ttttctttga tgaaagctgc tactgctgct tcatacgttt ttttatctgt tccatttgcc
     1201 ataaaagaag gccccccttg agtagctatt tggcttgttt taactgtttc gttaaattga
     1261 tgatttcaat cgtcaattca agctcaagct gtttgtttcc agttcgtctg gcttcttgct
     1321 gcatttgttt ttttaatgtt atttcatcag ctaagccaga tttgctgatc acatctagca
     1381 agtcttggat ttctcgtggc gaactttctt ctgccaccgg taaatattcg atttccacca
     1441 ccaaccgcct taaattttca tcttgcaaaa agtctaaaaa ctctgcaagg ttgaattcat
     1501 aatgttgtga gacatatgca tcgaataaaa aataaacttc tgcatatgca tcatgaataa
     1561 actgaaattc tcgttctttt aaaatattac ggacagaaac ttctttaaat gcacgatata
     1621 atagaatctg ctctgccttt tctacttgcg tcaaaggtct ctttttccgt gcagtttctt
     1681 gtctcgtaac ctgagtgtgt tgactttcct gaggttcttg acgattttgc tgatttgtcc
     1741 ttcgaagatc gcgcagttgt ttttgtatcg tctctctgcc gatctgaaac tctgttgaca
     1801 gttgagatag atacatatct tgttcgattg gtgaaacaac tcggctaagt tcaactagta
     1861 attcttcgag gtattcgatc ttgtcttttt cattatctaa atttttccct tgttttaaat
     1921 aacgtgattt aaaagtaaag actgtttcgc gcccatgagc cactaattct tggaaagatg
     1981 cttctccata cttacggaga tattcatcag gatcaagctt ttctggaacg actacgacag
     2041 aaagtgctaa tcgactgttt tgccgaagca attcaatccc tcgattggtt gcttcaaaac
     2101 ctgcgttgtc tccgtcatag caaaagacca attctttcgt cagcttttcc atttgttgga
     2161 tctgttgagc tgttagactt gtccccatag aagctaatcc agttttgata ccagcctgcc
     2221 atgcagcaat cacatccata aacccttcga acaacaaaat ttctccgttt ttccggatat
     2281 cgcttcttgc tttatcaaga ttaaataaaa cttgtctttt attgaacagt tctgtttctg
     2341 gactattcaa gtatttgggc tgatcttttt cctgcccttt ttcaggtgca agccagcgtc
     2401 ctgaaaaacc gatcgtctgg ccttgagaat tacgaagtgg aaacatcact ctttgataaa
     2461 aacgatcaga catacggcca tcatcatgcg tgacaaacaa tccagagcgt tctaacaatt
     2521 catttgaaat tgattcattc ttgaatactt gctgaagaaa agaacgctca ttaggtgcaa
     2581 aacctatgtt gaattcttca attaattcta aagacaaccc acgactttgc aaatattcta
     2641 aagctggctg tccagctttt gtatgcaata gcatgtgatg atagatttcc gctgcttttt
     2701 catgagcagc aatcagtttc cccgtagcag attcaggatt ttggacagct atagtacgct
     2761 gacgccattg atcttctaac ggtatctgtt cgaagtctgc cacttttacc actgcttctg
     2821 gaaaacttag accttctagt tcttgaagaa aagtaaacac gtttccgcct ttaccacaac
     2881 caaagcaatg aaaaatctgc ttgtcttctg caacagaaaa agacggtgta cgttcttcat
     2941 gaaaaggaca cagccccaag taatttttac cagacttttt caactggaca tattgtccaa
     3001 tcacctcaac gatattcgtt tgactgcgta ctgtttcgat aacctcttga ggaatccgtt
     3061 gagccatttt ttcaccccct gctctattga tatcttttgg gtttaacaga agatatttca
     3121 ggagaataac cggtaaaaaa tcgcactaac tgaaatcagt gcgattttaa aggacacaat
     3181 tatacatgtt tgattgtatc atatttcatt gctttttcaa gtataacaca tttctatctc
     3241 attaagcatc aatttgctac tcttttcagt aactgatttt gaatatcagc cttccagtac
     3301 aatactcgaa gaacaatatt tacgagcatg aatacaaaga agtatcctac gctctctccc
     3361 aaccctaagt ttacttccaa aattccatgg aacagcattg cagctacata tatccctacg
     3421 atcgtcgtta ctgctaggaa gatgcgcaat gggttgaagg gataacaagc ttttacaact
     3481 gccatgcaac tgatcccaat cagcaagtaa tacatcaaag tagtagtttc agcatttccc
     3541 catgcgaatg tttttccaag tagatacacc gcaatgatat tcactactac aagcaaggca
     3601 tttggtaaag cattaataag tgctgtaggc aaataacggt aagtgatttt tcttttatct
     3661 tgttcaaaag ataaaaagaa ggaaggatac ccttcaatcg ctaaatcaat cagcgtgatt
     3721 tggataggga taaacggaaa tccaattgct gtaacggcac atataatcga taaaataaat
     3781 gaataaatcg ttttgataaa aaagatgcct gagactttcg tcacattgtt gaccactcgg
     3841 cgtccttcaa acaggacttc tggcaatgta gtaaaatcag agtccaacaa taccagattc
     3901 gcaatctgac gtgttgctcc atcaccttct gccatagcaa tgctgcaatc tgcttctctt
     3961 aacgccaaaa catcattcac accgtcgcct gtcattgcaa ccgttctgcc gctttctttc
     4021 aattcattta ccagcaattt cttttgctgc ggactcactc gtccaaaaac cgtatagcga
     4081 tgagcagctt ctcgcacttc catttcttca gtaatttgag aaagatcgac atacgcatca
     4141 taattcggta atcctgcacg tctagcgata ttggagacag taaaaggatt gtctccagaa
     4201 atcactttca gatccacacc ttcctcatga agataagcta gtgtttcttg agcgtttttt
     4261 cggattggat catcgatttc aaacagagca accggctcaa caaatgctgg tttttcttcg
     4321 ttcaattgct gatttggagc gatccccaac ataagtacgc gatagccatt cgcttgggct
     4381 gtttttagac tgtccggaag ccgatcttct tccaccaaac gttcaggcgc tcctacgtac
     4441 acgactccca gttctggaaa ttccatcgcg ccccatttgc gatcagaaga aaatgcgact
     4501 ttgtttgtca catcataccg attagaaagc tcaaaataat ctctcatagc ccgcatagtg
     4561 atgttgttat cactgctttc tgccagatac gtccccataa tttctgggat ggctttctcg
     4621 tacagcggat gtagtgcttc tacccgctgg atactcattt tcccttcagt aatggtccct
     4681 gttttatcca agcataatgt atcgacatgg gccagcgttt caattgaata catgtcttga
     4741 acaaggattc gtttttttgc cagtttggtt acccctgtcg ttaatgcaat acttattagc
     4801 aataccaaac cctttggaag catgcctaac aaagctgctg cagatgcaat aacagaaatt
     4861 ttgatagaag catcgcggat gaacagtgct tccaaaaata gtatgatccc caaaggaata
     4921 atcacgaaac tagtgaattt ggatactttg cgaatcgaat ctacaagttc agaacgaata
     4981 gctttgtgag tctttgcctc gctagtcaag cggacagcat aattatctgc acctactcgg
     5041 ataacttcag ccatgacaga accgcttgtc aaaaagcttc cagaaagcaa ttcagctcca
     5101 gtttctttga caatcaaatc agattcgcct gtcaaaagag cttcatttgc ttctgcccgt
     5161 ccagatataa cctgtaaatc ggctggtatc tgttctcctg ctccgatttt taccaaatct
     5221 cctaatacca gctctttagt agaaatcgat tgttctttac cgtcccggat cactagtact
     5281 tctgattggg aaacaatcga taatttccgt acaagatttc ttgcatgtat ttcttgataa
     5341 attccaataa cgatattgac gacaataatt gctaaatacg ccatgttgga atacgcacct
     5401 acaaataaca gacaaatccc aataccaaag ttcagaaaat tgaataacgt caatatatta
     5461 tcgctgaata tttgttttgt actttttgat gcatcttcct ggtagtcatt ttgctggcca
     5521 gaagccattt tctgaactac ctcttgctgt gtaagtccat tcattttctt tttctccatt
     5581 ttccgccaac tttcaaaaat aatcacgtgt ttaagtctag cgtgttccaa gattaaaaac
     5641 aacttgtttt tataaaattt atagaaaaac acacgaatga ttttacaaaa aagatgccaa
     5701 cctttttgtt tgaatcaccc gtgtgctgtt tttattatca atctatcttt acttatattg
     5761 ttcctccaaa cggctcatcc accaacctag tgctgtaccg gctataaaaa gaacaggaca
     5821 agctagtacc ccagcaactc ccaatcccgc gtaatcggta ggaatgatca taatcgttga
     5881 agcaaaaact acacctaaaa tgaaatggaa tagcttgcta tatgcctttg agaaaatgta
     5941 atccatgatt tttgacaatc caagaacaca aagaatccca ccaattccga tcggcacaat
     6001 cacttcaagt cgtacttctt taaatccgtc agccatcgct ttatacaatc ccatatacac
     6061 caaaaaatta gaagggctca atcctgggac gatcataccc aaaccaatca atccgccggc
     6121 aatcagccat gttccaaaat tctgatcgac ttgcccgtca aataattgag cgccgtataa
     6181 taaaaacaaa taagccagaa caaaagtaac aaccatgatg atcacatcag aagtatctct
     6241 tccttttttc cctgcttctt tccataacgc aggaacagtt ccagcgatac atccgacaaa
     6301 aaaccataat atcgttgttt catacgcacc taataaaaaa ctgactaaaa aagaaagtag
     6361 aaagatgcct gtcactccgc ctagaccaac aggcagaaaa aacaaaacat tttctttgaa
     6421 attttttgta aggtgggcta aaaaagaaat cagccgctca tacatgccaa agacagctgc
     6481 taatgcacca ccgctgactc ctggcaagat aaatcctgaa ccgataaaca ttcccttcac
     6541 aaaccgtaac atccagtctt tggttgtgta ccccacttct tgattttcca ttttttgcct
     6601 tttttccact atttgcgcac tccttctttc atttaatttt attcatgatt gaacatttcc
     6661 tgctatttta cttaaagact ttttcatagg tctctattat tttttcattc attttgacaa
     6721 tatcaggata aaacatgtta cctccattat gatagagata gccgccattg taaagcaagg
     6781 actggatccg ccaatatcgg tattgtttcc cttcctcatt tcctaaaagt gggctcagca
     6841 catctttaga atacgcttct gctaattcta ctgtgttttc tccgccatga ttttttacat
     6901 aggcaatata atctttccca aaattatatg cttggacagc tgtccataaa tcgcatcctt
     6961 gtttctttgc ttctactatc atttccgcca aatatttgac accttgatca atactgtcag
     7021 ctggattatc aatgatctca gcttctccat gcagactttc cgagctctgc atgagatctg
     7081 tcccttttcc ttttgattcg gtcaatatta tcgccttgat caaattctcg tagttttcca
     7141 tatgataatt ttttgcagct gttgttattt ctatttgata gctttccact cgttttaccg
     7201 aacgataagt tcttcctcat aaaaacaaca ccgcaccaat aaccgtcaga atgatcatgt
     7261 aaaaaagaat acggataata gataatcgtt ttcgcttcat ttcatcacct agttattttg
     7321 acatcttttt ttatcttact atttctttat cttcttggct atttcttcct tgatttctgt
     7381 gtacgcctta agaaaaaatg aattttcctg tattttttga catcttacga aaatgatcgt
     7441 tgtttttcag taaaaacaat tgcccaatga ctgatataat cgttatactt tacacagtag
     7501 gtgattatgt gaaaaaagaa aagaaatatg caaaaatgcc agatgggagc gaaatttact
     7561 atgaaaagag cggtcaaggc ttccctctct ttttactgca tggcaatgac ggcagcggcc
     7621 gctttttttc agaacaggtc cctgtactcg aacgctacta taccgtgtat ctagtagata
     7681 gccgaggaca tggtcgttcg acaaacgaag catctatgtt gaattttcag ttaatggccg
     7741 aggatctgaa tacgatcatg ttgctggaaa aaatcgatca ggcagatttt cttggattta
     7801 gtgatggagc aaatctagca ctggtatttg ccagcagctt ccctaaaaaa gttcatcgat
     7861 tgattttaaa ttctggaaac acgcttgtta aaggcgttcg tttttctgcc agagtcatca
     7921 gtaatattca ttatgcatgg gtatggttac tctctttatt tcgcccaagt cttcgcaaaa
     7981 atcttttagt tatcaaactt ttgctgcatg acatcgggct tacagaaaat gatttaaaaa
     8041 aaatcaactc ccccactttg atcatcgtgg gaaaaaaaga tgtaatcaag ctaaaacatt
     8101 ctttgtatat agctaaaact ataccaaaag cttcctttgt gcttgtgaaa gaacaaggac
     8161 atgaattagc tagaaaagat cctgaacgct tcaatcgaga agttcttcaa tttctttctg
     8221 aaacataact attaggaata gaaggtgatt tatttgttaa aaaataccat acaatggctg
     8281 aaaaaccacc taggactgtt caaaacaatc tttttgattt ctgtcattgt cattattgtc
     8341 ggtgaattga tgtccatcgg taaaactcta tctatccagc aattaggaga aacgtttgcc
     8401 actatacctg tatggaaaag cggactaatg ctattgattg gtcttatctc tgtgttacct
     8461 atggttggtt atgatattat tttgaatcgg atgcttgaac aaaagcagaa tcctcgttac
     8521 ctgtttgaga caagctggct gatcaatacg atcaacaata ttgccggttt cggcgggttt
     8581 gttagtgtgg ggcttcgctc tgaactatat ggacaaaaga aagagagcag aaacgtgatc
     8641 caagcattat cgaaagtttt cttgtttttg atggctggac tttctattta cagtctgctt
     8701 tcttttttac ttgtagtctt cacgccagtt gctccttttt tgaagcaata ttggatatgg
     8761 ttgatcggcg gcggtctata ttttcctgtc gtctttttat ttactacttt caaaaagaaa
     8821 ggattccttg gtggtacctc acttaaaact caaggacagc tattgctagt ttcattgttg
     8881 gaatggaccg gtgtcctgtt tagtttcctt tctgttggtt atttaatgga aatccggatt
     8941 gatctgtggc aaacaattcc attattcatt gctgcttcag tgataggaat cgtgtctatg
     9001 attccaggag aaattgggag tttcgatgta atgatgatta tcgggttgtc cgctattggt
     9061 gtgccaagag aaatagtcgt tgtgtggatc ttgctctatc gcctgttcta ctacatcatt
     9121 ccgtttttga ttggaatcgt ctttttcttc aaaaatatcg gttccacttt tgatcagcgt
     9181 tattctggta tccctaaaca attagcaaca gaaattgcgc ataaaatcgt ggttgtctta
     9241 ttatactttt ctggaatcat gctagtactt tctgccacta tcccgcaagc ttttacagag
     9301 tttcgctggc ttcatagttt gaacccattg aaatttcatt ttattatcca atttcctagt
     9361 atactattgg gatttctttt aatcattatg ggcagaggga tcgcagcacg tgtgaaacga
     9421 gcatatttgc ctacaatctt cttgattgtt ctagctttac tttatgtttt attaagtgat
     9481 tttagtttca caccggtggt tttcttgtct attctgcttc tgatcattct tgcttctaaa
     9541 aacgaattat ttcgggaaca attgatctat tcatgggagt ggcgtacgat cgatggaatc
     9601 attattggtg cactgtcctt gctttacatc gttattgggg tatacaatct gccggatttc
     9661 cctcatcggc gtcaccattt tatttccttt ttcttatttc cttctgaaaa gatttggttt
     9721 tcgggattgt tggccatcat cgctgttagt ttcatgatcg ttctatttgt tcatttcctt
     9781 caaggagaaa agaaacagat tggcgaagcc tttaatgaag aaaaggccct gaagatcctt
     9841 acaacttatg gaggaaactc cgatagtcaa ttgatcttct taaaagataa gcggatgttt
     9901 gcctatgaaa aagacggtga accaactgtt cttctccaat tcgcttgctt caataataaa
     9961 tgtattgtaa tgggtgatcc ttctggtaag aaagaagatt ttccagaagc aatcgaagct
    10021 tttatcgaag aaacagatcg actatgctat ttaccagttt tctacgaaac aagcgaagag
    10081 attgttatga ttttgcatga attcggctac gatttcatta agatggggga agaagcttat
    10141 gtagacctga atagttttac aacttcaggg aaaaaaatga agggcactcg tgctgtcttg
    10201 aatcggatcg aacgagaagg atttacattt gatgtcttac aaccaccatt ttctactgag
    10261 caaatgagta ctttcaaaaa tatctctgat aattggttgg gttcacgaaa ggaaaaaggg
    10321 ttttctttag gctttttctc tgaagattat ctacaaagag cgcctattgc agttgtcaaa
    10381 aatacccaag aagaaattgt cgcttttgca acgatcatgc ctacttacac gaacaataaa
    10441 gtaggaacga ttgatctgat gcgatatgat tctaaaaaag caccttctgg cagtatggat
    10501 ttcttgttct tgcatttgtt tgcctatatg aaagaagaac atatccaatg gttcaattta
    10561 gggatggccc ctctttcaaa tgtcggaact tccagaaaga gttttttaca ggaaaggatt
    10621 gcatacttgg tttacgagtt tggttctcat ttttattctt tccatggatt aagagaatat
    10681 aaaagcaagt acgctacaaa ctgggtctca cgttatactc tatattctag agatagttgg
    10741 attgcatatg ttatgattgc tttgctgatt attgataatg cgccagttga gcagacacaa
    10801 ggtagaataa gtggtttgaa aaaatggata cgaagaaagt attaaaaact gcataccgat
    10861 agatgaacac tctcatgatg aataaaagag actgaagtaa aacgaaagtt catgcttcta
    10921 caatctcttc ttatacaatt gaagaacaaa gagcggtcca aacagaaatc ctgtttggac
    10981 cgctctttta ttttaaacat ttttcattac caaaatattc gagtcgcgtt tcttatgctt
    11041 cgatctctgt aacgatacct gaacctactg tccgtccgcc ttcacgaatt gagaatgtcg
    11101 ttccgttttc aacagcgatt ggatggatca agtcaacatc aatcgtcacg ttgtcaccag
    11161 gcatgaccat ttctgtatct tctggcaatg tgattgttcc tgtaacatca gttgtgcgga
    11221 agtagaactg tggacgatag ttgttgaaga atggagtatg acgtccacct tcttcttttg
    11281 tcaacacgta cacttctgct ttgaattttg tatgtggagt aatggaacct ggtttagcaa
    11341 gtacttgtcc acgttcgata tcgtcacgtt ggatcccccg taacaacacg cctacgttat
    11401 ccccagcttc cccgtaatct aacgttttac ggaacatttc tacacctgtt acaactgctt
    11461 tttgtgtttc aggtttgatc cctacgatct ctacctcatc accgacacga acagcaccac
    11521 gatcaatacg acctgaggca acagttcctc gacctgtaat tgagaagaca tcttccactg
    11581 gcaatagtaa tggtttatct gtatcacgtt ctggtgttgg gatatattca tctactgtat
    11641 ccatcaattc cataatagca gcttcagcat ctggatcgcc ttgcaatgct tttaatgcgg
    11701 aacctttgat cacaggagtg tcatcgcctg ggaaaccata ttcgctcaat aactcgcgaa
    11761 cttccatttc taccaaatcg atcaattctt catcatcgac taaatcaact ttgttcaaga
    11821 aaacaatcag gtattttaca ccaacttggc gtgacaataa aatgtgttca cgtgtttgag
    11881 gcatcggacc gtcagttgca gaaacaacta agattgcacc atccatttgt gctgcccctg
    11941 taatcatgtt tttcacatag tccgcgtgtc ctggtgcatc aatatgcgcg tagtggcgtt
    12001 tttctgtttc atattctacg tgtgcggtat tgatcgtaat cccacgttca cgttcttctg
    12061 gagctgcatc aatactagca tagtcttgag gatttgctag gccttttttg cctaatactg
    12121 tagtgatcgc agcagttaaa gtagttttcc catggtcaac gtgtccaatc gtaccaatgt
    12181 taacgtgtgg tttacttctg tcataatgtt cttttgccat aatttacaaa cctcctaaat
    12241 aatatagatc actgaaaaat ttcagctctc acctttttat tatagtcttt ttttgactaa
    12301 atcaaaaaga atacgtccga cttacttttt gtaagattcg aactaagctt attatacgtc
    12361 aatattggtc aaagtcaact aagagcactc gtctgtctta tacgcgaaaa aagtaccttt
    12421 tataaataaa aaaatgtgtt ttttgagaaa aacttcaaga tagtccttaa ctttcttttg
    12481 aagcaataag aacaaaatga ggaaaagttt aaaattcagc tgttgccaac aactttcaat
    12541 agacgaaatc ttctatttcg ttaataataa ggaaagaggt gaagtacatg aaaaaattaa
    12601 ttggcgtact tgtcgtttta ggcatatttc tcggcggaag ttttgcagct tatcattatt
    12661 tttacggcgg cgaagcttat tatacaaaaa tcacgacgaa tggcgaaatg tcagctaccc
    12721 aagcagataa cggagagaaa ttcacgactt atacctacaa gcaagcggct tataataaag
    12781 atggtgaaaa aaaacaggtg acgcttcgcg aagaaagaga acatccatta aagatgaatg
    12841 cgtatcttaa actcaaagtc aatcctcgta aaggtgtatt aagctgggaa gaaataaagg
    12901 cttctgaagt gcctaaaaat gcggcagaaa aaatcgataa ttagtaaatg gtcttaaaat
    12961 tttattagat aaaaagaggc tgggacagaa gcgtttaact ccaagaaata agaaggagtt
    13021 gcttctgctt ccg
//
LOCUS       DACEPW010000067        12851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEPW010000067 DACEPW010000000
VERSION     DACEPW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.068"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            254..580
                     /locus_tag="IW819_001487"
     CDS             254..580
                     /locus_tag="IW819_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244598.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(697..1041)
                     /locus_tag="IW819_001488"
     CDS             complement(697..1041)
                     /locus_tag="IW819_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244599.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1117..2364)
                     /locus_tag="IW819_001489"
     CDS             complement(1117..2364)
                     /locus_tag="IW819_001489"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1244600.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IW819_001490"
     CDS             complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IW819_001490"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1244601.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            3397..3924
                     /locus_tag="IW819_001491"
     CDS             3397..3924
                     /locus_tag="IW819_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1244602.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            3987..5357
                     /gene="radA"
                     /locus_tag="IW819_001492"
     CDS             3987..5357
                     /gene="radA"
                     /locus_tag="IW819_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1244603.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            5391..6548
                     /locus_tag="IW819_001493"
     CDS             5391..6548
                     /locus_tag="IW819_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1244604.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            6718..8178
                     /locus_tag="IW819_001494"
     CDS             6718..8178
                     /locus_tag="IW819_001494"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1244605.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            8500..9036
                     /gene="cysE"
                     /locus_tag="IW819_001495"
     CDS             8500..9036
                     /gene="cysE"
                     /locus_tag="IW819_001495"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1244606.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            9033..10445
                     /locus_tag="IW819_001496"
     CDS             9033..10445
                     /locus_tag="IW819_001496"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1244607.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            10442..10837
                     /locus_tag="IW819_001497"
     CDS             10442..10837
                     /locus_tag="IW819_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1244608.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            10830..11690
                     /gene="rlmB"
                     /locus_tag="IW819_001498"
     CDS             10830..11690
                     /gene="rlmB"
                     /locus_tag="IW819_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1244609.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            11694..12224
                     /locus_tag="IW819_001499"
     CDS             11694..12224
                     /locus_tag="IW819_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1244610.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            12301..>12851
                     /locus_tag="IW819_001500"
     CDS             12301..>12851
                     /locus_tag="IW819_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1244611.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIK"
BASE COUNT         4222 a         2390 c         2810 g         3429 t
ORIGIN      
        1 ccgtttacca agaggttgtg agactgtcgt ttaactctga atcacaagga acaatttcaa
       61 aaaacagttt ctcatgtttg ttgaatattg tgacttatga ttgaggagtt ggcagaagaa
      121 caccatttat tcggaaaaag gggatgtgac acgatttttg tcacattccc tttttttctc
      181 aaaataatga aactggaaaa ttttcagtag gtaagctaaa attcatttaa gaaaaagaat
      241 ggggggataa tagatgctgg aagaatattt tcaggaagtc gggttgacac tcgttgaatt
      301 acccattgaa ttttcagatg atcggttgat ttatcgtggc tatatcaaag aattgccaat
      361 aatcaatgcg gaaggtgaaa ctagaaaagc gatgcagcaa aaaatagctg gtatgtatca
      421 ggtgtatcgg gaacagctac tggctgaatc agcggaagaa gaaaaagaaa aaacgatgaa
      481 cttgtccatg gatgagctgc ttcgttatta tgatggcgaa acatttgacg gattttcgct
      541 tcttaacgaa gaggaaacag atttaccagg cagctggtag gcgaacgccg tttatatgga
      601 aaaagaggct gtgacaaaat ctttgtcaca gcctctaaaa agtccggcag ttttttgccg
      661 atcttcaagg cactctactt atcgtacgga tattttttag cggaaagtcc gattcaagtg
      721 gtttttattc agctgatatt tataagataa gtgaaaaatc ccagcaatca gtagcgtaat
      781 cgtataagcg attgaatgat cgaaaaaaga atagtaattg attgtcgcta ataagcataa
      841 tgcaaccatg taactaatgt gcaagcgtgt aaccagccct ttttgcccga ttttgtcaaa
      901 atactgcgac catttttccc gttgactccc tatgcttcgt tttttgaatt gaaagaaaag
      961 ggataaaata ccgactccca gaaaagcagc gatattcaga gaaataaggt aaataaaaaa
     1021 aatcattaaa atttcaaaca tattgacacc tttctagttg gatagaacct ttacagaaaa
     1081 aaggcaaaag gcgggaataa tgtttcacac gaaacattat tcgcggattt gtccatcgcc
     1141 ataaatgata tattttgtgg aagttaattc atttaacccc attggaccac gggcatgcaa
     1201 cttttgagta gagataccaa tctctgcacc aaaaccaaat accgatccgt ctgtaaaacg
     1261 agtggaggca ttgacataga cagccgccgc gtccacttca tttaagaaac gttgactagc
     1321 cgcataacta tctgtcacga tcgtttctga atgtttagta ttatgccgat tgatatgtgc
     1381 aattgcttca tccaaagaat cgaccacttt gacagcaagg atataatcta gatattcagt
     1441 atcccaatct gtgtcttctg ctaaagcagc cgtctctaga tattctaacg cacgagcatc
     1501 cgcacgtaaa gagaccccgt gttctaccag ttctttttca atagctggca agaaaaaagg
     1561 tgcgatctca gaatggatca gcaatgtttc tgccgcgtta cagacagatg gacgggaagc
     1621 ttttgcattg acgatgatgt tcactgccat agataactgt gcttctttat cgatatatac
     1681 gtgattattt cctgtacctg tttcaatgat aggtaccgtt gcctgttctt tgactcgttg
     1741 gattaatttt gctccgcctc gaggaatcag cacatctaag tattctgtca atcgcatcaa
     1801 ctgtcctgca gtttcatgag aagtatcatc gacaaactgg atggcataag gagattcgcc
     1861 agcactgacc agtacttgct gcaataattg gactaataat tgattcgaga agaatgcttc
     1921 tttgccaccg cgaaggataa cggcattccc tgatttgaat gctagactag ctgcatctgt
     1981 cgtgacattt ggacgagatt cgtaaatgat gccggtcacg cctagtggca cgcgcttttt
     2041 gccgattctc aatccgtcgg tgttcgtcca catctcatct acttttccaa taggatcgtc
     2101 taacgaagca attttttcga tctctgtagc catggcattg attcgctcag gtgtcagctg
     2161 caagcggtct tgcatcgttt cactgatatt gttttcgact gctttttcta agtctttatg
     2221 atttgcttca atgatcgctt gttgattctt tttgatggtt tgtgccattt gaacaagtag
     2281 ctcatttttg cggtttgtag gcataagtgc tagttgttgc gcgctatctt ttgcctgttg
     2341 tcctaattgc atcaagtcag tcatttcaag tcctccttaa ataaagtacc tacttctttt
     2401 cctgcaagaa tatcaaaaat aattgccgga ttttttccat ttgctaaaat cattgcgctg
     2461 tttgcttcaa atatcctaga agcggctttt aatttactct gcataccgcc tgttccaaat
     2521 gtgctaccag caccacctgc ttgtgccaga atcttttcat cgattttatc gatatagcga
     2581 tacatctgag cctcgggatt tgtagtcgga ttatctgaat aaaacccgtc gatatcagaa
     2641 aggacgatca aaaggtctgc cgcgacaagt tttgccatga tggctgacag ctgatcatta
     2701 tcgccaaatt ttgtcagatg atctaattca tccactgcta cagtatcgtt ttcgttcacg
     2761 atagggatcg tgtccataat caaaagctgt tccaatgtat tcctcacatt tttacgactt
     2821 tcaggataat ctaccacgtc tcttgtcacg aggatctgtg ccgtctgttg attatataca
     2881 ctgaatcttt gattatagat attcatcagt tcggcttgac caacagccgc cgcagcttgc
     2941 tgttcaggaa tcaaagtcgg acgttctgac aaatgtaatt tatgcatacc aacgcctaca
     3001 gctcctgatg agactaagat cacttccttt ccccgatttc taagatcaga aaggacaaaa
     3061 gctaactgat cgattgccgc aagattgata tttccattgg gataaatcaa tgtacttgtt
     3121 cctactttga caataattcg ttttgcattt ttaatttctt tacgcatgtg acacctcaat
     3181 caaatatcca ctcattaatt tcgttctatt ctacttgttt tgacgagtaa agtcatgtaa
     3241 aaaatagttt ttttgtacaa cttgcagtcg tttggaaaaa gaacgaatta atggtttact
     3301 ttaagatagc agaatttctt gaagaatttg taaagaaagt cttttacagc ctcggatatg
     3361 ttagaatagg agtagtttat ttagggagga aaaccggtga gaaaaagagg atttgaagtt
     3421 atttctaaat ataaagatca aaatatcaat ctaccgcagc gcgctacaca tcatgctgct
     3481 ggctatgatt ttgaagcagc agaagaggtc gtgattccaa gtatctggaa agcaggctgg
     3541 aaaaaggggt tggaaacagt cgcagatgga ttgaaaaaag atgtatcaga aaatgaaaaa
     3601 caattgaaac caaccctcgt gcctactgga atcaaagctt acatgggaga agatgaattt
     3661 ttgcagcttg ctaaccgttc aagcaatccg ttgaagcggt ttttagtgtt gacaaacgga
     3721 gtaggtgtga ttgacagtga ttattacaat aatgaggcaa atgaaggcca tatcatgttc
     3781 caattcacca actttggttt caaggatgta gtgatcaaaa aaggcgagcg tatcggccaa
     3841 gggatctttt tgccgttttt aaaagcagat caagatcaaa cgactgctca acgtcaaggc
     3901 ggatttggtt cttccagtaa ataaaacgtt aaagatactt aagcttgatt taatgtctca
     3961 tgattaggat aaaaggaggc ggtacaatgg caaaaaaatc aaaagtacaa tttgtttgcc
     4021 aaaattgcgg gtatgtgtcg cctaagttct tgggacgctg cccaaattgc ggaaaatgga
     4081 atacgatggt cgaagaaatc gaacaagata caacggaccg cagaactcgg acaagtttga
     4141 caggagaaaa agcaaaacca acaaaaatcg cagacgtcgt ccctaaaaaa gaacctcgga
     4201 tcaaaacaaa attagaagaa ctgaaccgtg tgttaggcgg aggtgtggtg ccagggtcta
     4261 tggtcttgat tggtggcgat ccaggtatcg ggaaatccac tttgctgtta caagtttctc
     4321 agcaattagc agccataggc ggaaaagtct tatatgtatc cggcgaagaa agcgcagagc
     4381 agataaaatt acgcgcggaa cgcctcggaa gtatcaacac agaattttat ctatacgctg
     4441 aaacagatat gaacgaaatc agtcgtgcca tcgaacatat ctcccctgat tatgttatca
     4501 ttgattccat ccaaacgatg acgcagccag acatcacaag tgtggcagga agtgtcagcc
     4561 aagtaagaga aaccactgca gaactactca aaatcgctaa aacaaatggg atagccattt
     4621 ttatcgtcgg tcatgttacc aaagaaggct cgatcgccgg cccaagaatg ttagaacata
     4681 tggtggatac ggtgttatat tttgaaggtg aacaacatca tagttttcgg attttgcgag
     4741 cagtaaaaaa ccgatttggc tcaacgaatg agattgggat ttttgaaatg catgagcatg
     4801 gattggaaga agtcgctaat ccttcacaga ttttcttaga agaacgccta gacggtgcaa
     4861 ctggctctgc tatcgttgtg gcaatggaag ggacacgtcc aattttggta gagatccaag
     4921 cattagtcac acctactatg ttcggaaatg ccaaacgaac aacaacaggt cttgatttca
     4981 atcgggtatc tctgatcatg gcagtgctgg aaaaaagagc tgggttgctt cttcagaatc
     5041 aagatgctta cttaaaagct gcaggtgggg taaaattaaa tgaaccagcg attgatttag
     5101 ctatcgctgt cagtattgct tcaagctata aagaaaaagg aacacagcca acggaatgtt
     5161 tcatcggtga aattggactg actggagaaa tcagacgtgt cagtcatatt gaacaacgag
     5221 tcaaagaagt ccaaaaactc ggcttcacaa aagtatacct acctaaaaac aacttaggta
     5281 actgggaagc gccaaaagga atcgagatag ttggagtagc aactttggct gaaacgttga
     5341 agcgtgtgtt ccgctaacta ataaatgatt gtcgaaacgg aggaaaaacg atgcaaaaac
     5401 gtgtcattac tttgctgatg atcattgtcg gcgcaagctt gggtatttca tttttaccct
     5461 cagcttggca ggcaatcggt caacagtcaa acggatggct gaataataca tttaccaaca
     5521 gcctgttagg cgcacttatt ttctttttat tatctttgct gttggccaaa catatctcag
     5581 ctattatcaa acaagtagaa caaaaactaa gtgaggtcag cttgacttat cttctcttcg
     5641 gtgcgatcgg tgcgatcatc ggattgactt taggtgttgt gatctcgacc cctctttaca
     5701 atatggaaat tccattcgtc aatagtgtat tgccgatttt actgatgatc atcttaggct
     5761 atctgggatt gagaatggga acgacacgaa tcgaagaatg gaagaaagtt tttggttcac
     5821 gaagcaaaaa aatcgagcaa gaaacagcta gtcaagttga gagtcaactg ttagaacgaa
     5881 aatcaggaga aaatttccat aaatataaaa tcttggatac aagcgtgatc attgacggtc
     5941 ggatttatga tattacaaag actggattct tagaaggaac cttgatgatc cctaattttg
     6001 ttctttacga actacaatac atcgcggact caggggatag tttgaaacgt gtgcgtggtc
     6061 gtcgcggatt agatatccta aatgccttgc aaaaagaaga gggtatatct gtcgaaatgt
     6121 acgatggaga ctttgaagat atcagtgaag tagacagcaa attgatcaaa ttggctaagc
     6181 ttttagatgg tgtcattgta accaatgact ataacttgaa taaagtctct gaattccaaa
     6241 atgtacctgt tcttaatatc aatgcgttgg caaatgccgt aaaaccagtc gtcatccctg
     6301 gtgaaacgat gaatgttcta gtagtaaaag ccgggacaga acgccaacaa ggagtagcct
     6361 accttgatga cggcacgatg gttgtcgtag aagatggaca gcattacatg aatgaacgta
     6421 tcgaagtcgt tgtaacaagt gcccttcaaa cagctgctgg acgcatgatc tttgctaaac
     6481 ctgcacatgc aggacgagga atcgacaata aaaaagagac agagaaaaag actaaacaat
     6541 caaaataaaa aagaaattca gccaactggt aaggaaattc aaccagttgg ctttactatt
     6601 taccttagaa aaggtaaaat ttgtgctgag tggctttaaa taaatgatat ttgagaagcc
     6661 gtaactgacg gaatttttgc tgttagtcaa agtctagttt gaaagaagag gaagaaaatg
     6721 acgaaagtac gcgtacgcta tgcaccaagt ccaactgggc acctgcatat tggaaatgca
     6781 agaactgcat tattcaatta tttgtttgcc cgccacaatg atggagactt tattattcgt
     6841 attgaggata ctgaccaaaa acgtaatatc gaagatggcg aaaaaagcca gttggaaaac
     6901 cttgcttggt tgggaatgaa ttgggatgaa tctcctgaaa atcctggaga atacggccct
     6961 taccgtcaat ctgaacgtaa agaaatctat caaccgctga ttgatcagct gcttgctagc
     7021 aatcgagcat acaaatgcta ttgcacagaa gaagaattag aagcagaacg ggaagcacaa
     7081 cgcgcaagag gagaaatgcc tcattacgca ggaacatgtg cgaacttgac acctgaagaa
     7141 caagcagaaa aagaagcaca aggtctagaa tctgttgtac gtttccgtgt gcctcgcaat
     7201 acagaatacg catttacaga tatggtgaaa ggcgctattt cgttcgaatc cgataatatc
     7261 ggtggcgact ttgtcataca aaaacgcgat ggtatgccaa cttataattt tgctgttgcg
     7321 gtcgatgatc atttgatgaa aatcactcac gttttacgtg gagatgacca tattgccaat
     7381 acaccaaaac aactgatgat ctatgaagca tttggctgga caccaccaac cttcggacat
     7441 atgacattga tcatcaacag tgaaacaggt aaaaaactaa gcaaacgtga cgaatcaatc
     7501 ttgcaattta tcgaacaata tcgcgaatta ggctatctac cagatgcaat gttcaacttt
     7561 attgcattat tgggctggtc acctgtgggc gaagacgaaa tcttcgaaca ccaagaattg
     7621 atcaaaatgt tcgatccaaa acgcctaagt aaatcacccg cagctttcga tgcgaaaaaa
     7681 ctgcaatggg taaacaacca ttacatcaaa gcaatggatc tagatgcatt tactgacatg
     7741 tgtctgcctt atctaatcgc tgatggacgt gtagcagaaa acccagatga aaagacaatc
     7801 gaatgggtga agaagatcgt tagcctgtat caacctcaaa tgagctatgc tgcagaaatc
     7861 gttgatctgt ctacgctatt tttcaatgaa catcctgtat tgaatgaagc agcaaaagaa
     7921 gtacttgctg gtgaaacagt tcctactgtt ctccacgcat tcaaagcaca gctagaacaa
     7981 atggaagtag tagatgctcc aactgtcaaa gctgccatca aagctgttca aaaagaaact
     8041 ggcgtaaaag gaaaaaatct tttcatgcca atccgtgtag ctgtatcagg acaaatgcat
     8101 ggtccagaat tgcctgaaac gatcgaattg cttggtcgtg aaaaatcatt agcacattta
     8161 aatcaagtac tgaactaaac acgatgaaca gaaaaaagta agcagctgat cattacaaga
     8221 gagataccgg gtggtgcaag gtatcaatga atggttgccg aaatgcctct gggagtggtc
     8281 ttggcgaaac acagaaaaca ggaaaaactg ttttatagga aacaaagtag ccaagaacgt
     8341 ataacaggcg ttagctgtca gagataatca ttgattatga agaaaagtgg aaccacgcgg
     8401 aaagcgtctt ttgataagga aacttattta aaaggcgctt cttttcttta taagaagatg
     8461 atttcctaca taaaattctt acgaaaggag tggaaagtca tgagttggtt tacccgagcg
     8521 attgatgcag caaagagaaa cgatccggct gcacgttcga cagctgagat cgttttgaca
     8581 taccctggtt ttcatgcctt gttttggcat cgtttctctc attttttata tcagcaccgg
     8641 ctctttttat tggcgaagat caatgctcaa ttctggcgtt tcctaactgg tgttgagatc
     8701 catccaggag caacgattgg aactggcgtg ttcatcgacc atggaatggg aatcgtcatt
     8761 ggcgaaaccg cagaaatcga ggacgatgtg attttattcc atggcgtaac attaggcggc
     8821 acaggaaaag aaacgggtaa aagacaccca actgttaaac aaggagccat gctctctgcg
     8881 aatgcgcaga tattaggacc ggtaacgatc ggaaaaaacg ccaaaatcgg tgcaggtgcc
     8941 gttgtgttga aagatgttcc agacgacgct acagcggtag gagtaccagc aaaagtcgtt
     9001 cgaatcaaag gagaaaaagt gaggagatca gaatgataaa aatctacaat acattgacca
     9061 gaaaaaaaga agaattcatc cctatacaaa agggacatgt cggtatgtat gtctgcggac
     9121 cgactgtgta taattatatc catatcggaa atgctcgtag caccattgct ttcgatacaa
     9181 ttcgacgcta ttttgaatat cgaggctata aagtcgattt cgtatcgaat ttcacggatg
     9241 tagacgataa aatcatccgt acagcaaacg aattaggtat tacagcacca gaagttgctg
     9301 atcgtttcat ccgagcattt gaagaagata cgaaagcgtt gaatgtcgag cctgctacac
     9361 tgcatccaag agtcatggat catatgccag atattttagc atttattcaa gtgttggttg
     9421 aaaaaggcta tgcttatgaa tcttcaggtg atgtgtatta ccgtacccgt aaatttgaaa
     9481 attatgggaa attaagtgac caatccatcg atgaactgga agtcggagca agtcagcgta
     9541 caggggaaga acagcagcaa aaagaagacc cattagattt cgctttatgg aaacaagcaa
     9601 aacctggcga gatttcttgg gattcaccat ggggcaaagg acgtcccggt tggcatattg
     9661 aatgttccgt tatggcaaca aaacatttag gagaaacgat cgatatccat ggtggcgggc
     9721 aagatttaga atttcctcat catgaaaatg aaatcgcaca aagtgaagcg aaaacaggga
     9781 aaaaattcgc taattactgg atgcacaatg gctatgtaac gattggcgaa gacgatgaaa
     9841 aaatgagtaa atctctaggg aatttcatca ctgtacatga aatgatcaaa aacgttgatc
     9901 cacaagtctt gcgtttcttc atggcgacca cacaataccg tcgtccgatc cgctacagtg
     9961 aaacgacgat gaaagaagca gccaccaatt tacaacgact gaagaatacg tttgacaatt
    10021 tgaaattccg ccaagaaact gcctcagaga atttggcaga tgatgcggat aaaatcgctt
    10081 tgttagaaaa tttagaacag cgattcatcg aagaaatgga cgatgatttt aatgcagcaa
    10141 atgggatcac agtggtctat gagcttgcta agtggctgaa cacttatcta gaccagccga
    10201 aagtttctgc cgctgtcttg gcacaaggag aagctttatt ctcgcaatgg ctttcgatct
    10261 tcggtatttt atttacagca gaagagttat tagatgaaga gattgaacaa ttgatcgaag
    10321 aacgaaatca agcaagaagc aatcgtgatt ttgtccgaag cgacgaaatt cgtgatttgt
    10381 tgaaagaaaa agggatctta ttagaagata caccgcaagg aacccgatgg agaagagaag
    10441 catgagagat tacacacaat tgaacggttt agccttggct tatgttggag atgcgattta
    10501 cgaaatctat gttcgtgacc acctgattga tcaaggagaa acaaaaccca ataagctgca
    10561 tcgtgcggca acgaattatg tttctgcgaa tgcacaagcc tttttgattc agcaaatgct
    10621 ggaagaacat cttttgacag aagaagaaga actttattat cgcagaggaa gaaatgctaa
    10681 aagccatacg agcgcaaaaa atgctgatgt tacgacgtat cgggtcgcaa cagggttcga
    10741 agcgctcatg ggctatctgc atttgacaaa acaaaccgag cggatggaag aattgatccg
    10801 ctggtgtatt cagaaagttg gtgaaaaaaa tgggtaaaga aagaacaaac aaacatcaat
    10861 cgaaacgtgg atttgaagac aaaaaacaaa atagatattc aaataaaaag caaactggac
    10921 gaaatcagtc agtttctgat caaaatgaag aagcattaga agataatttc gtctttgggc
    10981 accatgccgc agtagaagcg ctacaacaag ggcgagggaa taaactcttt cttcaagaag
    11041 attcacgagg agaaaaagta gaagaactga aagaaatcgc acgtgaacaa gctgtgccag
    11101 tcaaatgggt tcctaaacaa aaattagaaa ctttgagcga tcacggcgtg catcaaggag
    11161 tcgttcttgc gattacacct tatgaatatt tatcccttga ccaactgatt gaacgaacaa
    11221 aagagaaaaa agaagacccc ttctttttga ttttggatag catcgaagat ccacacaatt
    11281 ttgggtcgat tttacggaca gcagacgcaa gtggcgtcga tggtgtgatc ataccaaaac
    11341 accgagcagt tggaatcaca cctatcgtaa caaagacttc cactggggca gtcgaacatg
    11401 tccctgttgc acgcgtgacg aatttggtac aagctgtgca acagctcaaa gaaacaggat
    11461 tttgggtatt tggcacagat atgaccggca caaacttcca aaaatggaat gccaaaggat
    11521 caattgcttt agtcattggt aatgaaggca aaggaatgag tccaggattg aaaaaagaaa
    11581 tggatgaatt gctgacgatc ccgatgaccg ggcacgtcca gagcttaaat gccagtgttg
    11641 ctgctggttt gcttatgtat caggcttttt cccaacgtca gggggagtag accatgaaaa
    11701 aacagctgtt gattgtagac ggctacaaca tgattggttc gtggccggaa cttgtccagc
    11761 ttaaaaagca ggacaaaatg gcggatgcaa gagaagctct tttacatcgt ttatcaaatt
    11821 atgcgaaata tgaaggaatg gaagtcatcg ttgtcttcga tgcccaacta gttccgggca
    11881 tccaacaaaa ttataagaaa taccagttgg atgttgtttt tacgaaagaa gacgaaacgg
    11941 ctgacagtta tatcgagaga atcgctggtc agctgaatga ccgtttgaca caagtgacag
    12001 tggcgacaag cgatttagca gaacagtgga tgatcttttc ccaaggagca ttacgaactt
    12061 ctgcttatga attatataag acgataaaaa aaacggaaaa aacaatagcc caagaaaata
    12121 aagatatgca ttttattgat ttccgacgta attctccatg gaatgaagag cagttaaaaa
    12181 aattgtccca aaaattggac gatttaatga gaaaaaataa ataacatatc gttctaatca
    12241 gttcgatgaa tcattgatga taagattcct ccataaacga atttatggag gaattatttt
    12301 atgtctgagg agttattgcg cgctgcacgt aatggtgacg aagaagcatt tgctgccctg
    12361 tatcaaaaat attttccact tttactacgt ttgaaaaaca cctattattt aagaaattat
    12421 gtttctgagg attgggaaca agaagcaagg atcgctttat atcaggcatt gagaacttac
    12481 gaaaaaagca aaggtgttag ctttggaagt tattataaaa gcattataag caatcaaatc
    12541 tattctctgt tgaggaagca gaacgcgctg aagcgtcgtg atcaaaaaga tgaagtctca
    12601 atcgaccaaa aaatagagac agaaggacca gactttttag cagagatcac attgcaagaa
    12661 cctttagcga ttcagcatct tttattaaaa gaagcaatcg aaaattgcga aatccagttt
    12721 tcaaaaatag aagctaaaat ttttcattat tatatgcaag gcgttgattt tgtagacatg
    12781 gctgaatgtt tagcaatgga tcacaagcaa gtaatgagcg cttacgggag agtaaaactc
    12841 aaaataaaaa a
//
LOCUS       DACEPW010000068        12808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEPW010000068 DACEPW010000000
VERSION     DACEPW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.069"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..634
                     /locus_tag="IW819_001501"
     CDS             200..634
                     /locus_tag="IW819_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1244612.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            735..1826
                     /locus_tag="IW819_001502"
     CDS             735..1826
                     /locus_tag="IW819_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1244613.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            1856..2533
                     /locus_tag="IW819_001503"
     CDS             1856..2533
                     /locus_tag="IW819_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1244614.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            2865..4160
                     /locus_tag="IW819_001504"
     CDS             2865..4160
                     /locus_tag="IW819_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1244615.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            4150..5610
                     /locus_tag="IW819_001505"
     CDS             4150..5610
                     /locus_tag="IW819_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1244616.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            5746..7242
                     /gene="glpK"
                     /locus_tag="IW819_001506"
     CDS             5746..7242
                     /gene="glpK"
                     /locus_tag="IW819_001506"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1244617.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            7256..9079
                     /gene="glpO"
                     /locus_tag="IW819_001507"
     CDS             7256..9079
                     /gene="glpO"
                     /locus_tag="IW819_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1244618.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            9080..9796
                     /locus_tag="IW819_001508"
     CDS             9080..9796
                     /locus_tag="IW819_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1244619.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            10102..12030
                     /locus_tag="IW819_001509"
     CDS             10102..12030
                     /locus_tag="IW819_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1244620.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            12156..12710
                     /locus_tag="IW819_001510"
     CDS             12156..12710
                     /locus_tag="IW819_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1244621.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         4297 a         2130 c         2901 g         3480 t
ORIGIN      
        1 atactcaaaa ctaaacgact ttttcacaac ctctgattca cggtgttcca gaaggagttt
       61 tgtcgaaaac aagccgaaat tctttgaaga tggctacttc tttttttcaa atctagacag
      121 aggaggcgga cattattccg tgttttttga caaaaaataa aaatcatact atgatatgta
      181 cgccaatgga ggggatgaaa tggaaaagcc aattgaaaaa gcgttagatg aatgtctttt
      241 ctttagcgtg aaaaagctag atcgcatgtt gaataaatta gctgatgaag cgtttagaag
      301 gacaggtctg gcaccaactt atggttttat tttattgata ttaaaagaaa aagatggaat
      361 accacaaaaa gatattgcac aaatgctcta ttccgcccca tcaacgattg ctcgttttgt
      421 tgaaaaatta gaatataaag gatacgtcaa aacagttagc gaaggtcgct tgtcattagt
      481 ttatcttaca gaacaaggac gccaatttgc taaggaagtc gatggtagct gggaagaact
      541 tcatcagtca tacaaagcag ttttgggaaa tgaagcaagt aaagaattag cccagacaat
      601 gaatgatgca acagataagt tacaaaaaaa ataaaatttt ttaagcaaat tagttgcatg
      661 tacaactaat cgagtggttt aatgatagat gattctcgat aggttaatca gagcgaatta
      721 tgggaggaat gacaatgtca caatttgatc aatcaattac tttaccttca ggagttcaat
      781 tgaagaaccg attgatgctg tcgcctatga cgacacaact cagctttttc aatggagtga
      841 tcaccgaaga tgagatcact tattataaac aacgttctaa aggtcttggt gctgttatta
      901 ctggtgctgc aaatgtccaa gatatcggaa aaggctggcc aggtgaactc agcatcgctc
      961 atgatgaaat gattcctcga ttaagtgagc tagcgaaagc aatccaagga gaaggcgcaa
     1021 aagcgattat tcagattttc cacggtggac gaatgacaag tcgagcaact ttatctggag
     1081 aacagcctgt atctgcaagt gcagttgcag caaaacgtcc tgatgcggaa acaccacgag
     1141 caatgagtga agaagaaatc gttgaaacaa tccaagcatt tggcaaagcc acacgtagag
     1201 cgattgaatc aggatttgat ggaatcgagt tgcatggagc aaatacgtat ttgatccaac
     1261 aatttttctc tccgcattcg aatagacgag aggatcaatg gggcggtagc ttggaaaatc
     1321 gttatcgttt tattgaagaa ttattaacaa cggttttctc agcggttgaa caatatgcta
     1381 aaaaaccatt catcgtaggg tatcgtttct caccagaaga atatgaaaca ccaggtatcc
     1441 gttttgaaga cacgatctgg cttcttgaac gattacgtga gtcaaaacta gattatctgc
     1501 atgtttcatt aaatatgtat gatcgaatag ctcgttctga taaatacaac gataaatcga
     1561 ttcttgaagt ggtccataac acgattcaag gaaagatccc tttagttggt gtcggtggga
     1621 tccgcaatcg cgaagatgta gagcaggtat tgaaatatgc agaacttgca gcaattggac
     1681 agcaaatgat cgtcgatcct gactgggcag agaaattatt ggaaaaccgt gaggatgaat
     1741 ttgtaacgaa accttttgaa gaagcatata aagagcttta tttgccaagc ccattatata
     1801 atttcttgaa agcacgttat caataacgca atttttatag aaagaggatg ggaaaatgaa
     1861 agtattgatc gtattaacaa atgtagaaaa gtacgaaaag atcgagcgtc ctacaggttt
     1921 gtggttaagt gaactgactc atttttatga tgtattagta aaaaatggga tcgatgcaga
     1981 ttttgtcagt cctaaaggcg gatatgtacc tttagaaccg caaagtgttt tgaatatgga
     2041 tgaaatcgat tgggcctatt atgaagatga agatttcaga aatcgggcct tgggaaatac
     2101 cttacgtcct gatgatgtgg atgcaaatga ttatgcagcg gtttattatg ctggtggcca
     2161 cggaacaatg tgggattttc cagaagcaat agatcttggg aaagtcgctt cacaaatcta
     2221 tcgaaatgaa gggctggtct cagctgtttg tcacggtgtt gtaggattgt tgccagttac
     2281 agatgagtct ggaaaatcta ttttagctgg acgtacagtg actgggttca gtaatgaaga
     2341 agaggaagca aatggtacaa cgaatgaagt accattctta gcagaagatg ctttgaaaga
     2401 aaaaggagca aactatcagt ctggagcagc tttttcagaa gtggttcaaa ttgatgggcg
     2461 gcttcttaca gggcaaaatc ctcaatctgc tcatcaatta ggcgaagcag ttgttgaagc
     2521 attaaagaat taaaaataaa tgatgtgtct attctaacta ttacattaca cacgctaaaa
     2581 ctcgtctttt cgttcttttc tgtttcctat ttggacagag gtacgtaaaa ggcgagtttt
     2641 tttcttttat aagagtacat tttttgaaac atctcttcag aaatgatact cttcaattag
     2701 gtccactagt acattattga gaagcagacc tagaacacgg tatgatggag agggttgatt
     2761 ctgctgcatc gctatgtatc ataaggttga aagcgagtta tgaatactgt gttttctgat
     2821 tgagaagtta gcagtagaac accttataag aaagaggaat gcagatgaac attgtaattg
     2881 tcggcgggtc gtttgcaggg attcatgcgg ctatttatct tcgccaattg atgccaacaa
     2941 gtgaaatata tttgattgaa aagcaatcga agttaggatg gattcccagc gggttcaatt
     3001 tgatattaaa aggaaaggtt accagtgaaa aacaactttc ttggatcacg aaagaagaac
     3061 tgactgaccg ctatcgaatc catgtttaca cagaaaagac agtaatagga ttgaaagaaa
     3121 aaaaagtcgt tttagcagga ggaaaaaaat tggattttga tcgtttgatt ctagcgactg
     3181 gttcaaatca aaagtttcga aatatttctg cagaatcaac gcttattcac ccagttaaaa
     3241 agatacaaaa cgtaacggct cttcagcaaa agatcatgaa tgcaaagaaa attgcaatta
     3301 ttggtgcagg acaagcagga atcgaaatag ctgaagggtt ggcatcaaga aaaaaacaaa
     3361 tccatcttta cgaaagccga aagtcaattc tttttcgtta tctagatcct gaaatgacag
     3421 aaccgttagt aaaggaaatg aaaaatcaag ggctttcact ttttttagaa gaacaagttg
     3481 tgtctttgac cgaaaatgaa accgctattg tagagacgga aaaaagaaag gagaactatg
     3541 atcttgtttt gctggctaat cattctcgcc ccgacaatca gatgtgggaa gaacaattaa
     3601 cattgaatga tgatggaacg atctgggtag atgactactt gcaaacgtct caaaaagacg
     3661 tttatgcgat tggcgatgcg atccaagtaa cttttcgtcc aacaaatgaa aaaatgtatg
     3721 tttctctagt aaataacgct gtccgtacag cacgaaatgt cagcaagaca attagtggct
     3781 cacagacaaa ggatcaggga acctatcgtc cagtcggcaa tcagtggttt ggctatttct
     3841 taggaagcgt aggtttgaca gaagaagaga gcatttttta tccccaaaaa attgaaacag
     3901 gatacgtaga ctacaagctt acagcaataa atcaaagcaa agttaaactc aaatttctat
     3961 ttgattctga aaaaaagatt cttggagcac agctccaaag caaaaaagag atgttccacc
     4021 ttcttgacca actgacaatt gcagtagaag aaggatggac gtttgaacag ttagaagaac
     4081 atgagctatt tttccagcca gaataccgtg tgccagatat ggtgtggtca aaggtggcga
     4141 atactgccta tgaagattga agcattatta gaaagaaaag agcagttaca gatcctgatt
     4201 ttgcgaaacc ttgttctgca agggggaaca gcctctatca atggtttgag agagcatgtc
     4261 caactgtcaa aagcctcgtt tgatcaatat ttggaagata ttgaattgat tggccgaatg
     4321 atggaaaaaa aagtagaagt acagcgaaat gagtatcagg cgctgcttgt gttggacgaa
     4381 gatgtaagtt tggaaaaaat tctattgttt ttattgcagg aaagcttgaa gtttaagatg
     4441 ctcgtctatt tattagaaca tcagcaagta tccatcgttc gattagcgac agcgtttaat
     4501 atcagtgagt cttctgtttt tcgaaagatc aaagaattga accagctctt agaggaattt
     4561 ggattacaaa tcaaaaacgg tcaattatac ggagaagagc tacagatacg atatttttat
     4621 tatgaactat tccaatatat cccggaagat cagcgaccgc tttttttgca aaatacacca
     4681 gaaaaaagac cgttcattct aggattggac cgtgtattag aaacaacgtt tacggcatca
     4741 gcagaagcac agattgcttg ctggctcggg attacgaaaa aaagactatt gaatgaaaaa
     4801 agtacgtacg caactttgaa agaaaagaaa ttactctatc agtcggaccg gctttatcaa
     4861 gcaatcgatc caatcatcgt gatgcatctt agtcgaacag cagcagaact gaatgtatac
     4921 gaaacaatga tgttttacag cttttttgtt tctttttcga tagtagacga agaaatattt
     4981 tatcagtatg atttgacacg gagcaaaaag ttaccaaccg ctgttttaga tacttacatt
     5041 cgggaaacga tgttatggca ttatcgtcct cgtcgtttga aaatcaaaga agaaaaggca
     5101 gtaggctacc aaatttccca gatcaacaat gaatggtttt tctttgttgg aaagatcgaa
     5161 gtctatgagc gtgatcggct tttggaacag cagcagaaaa tgctcgggca ttccttaaca
     5221 cagttactgg cgaaattaca ggaaacagcg gtgcagcaat tgccgaggaa aagagctgaa
     5281 gatagtgagc ttagttacct gatgatccag tatgccaatg ttcttttgat gatcgatttt
     5341 tatattgcaa aaccagtagt gattggaatc gatttagaaa gtctgccgat ttatcgtatt
     5401 gcttttcaac aatatctgat cagagaactg aggggaatcg gcggtattga gattggaagt
     5461 tatgaagaag gtaaagaata tgatttggtg atcacatttt gccagagaaa caaaaaacag
     5521 tgtgaatatt atctgtcaga atttgcgtca ccttacgaca ttattcgatt gaaaagaaga
     5581 atagagacgc tgaaaaaaga aaagaattga ttaaaaaata gcttcataat aaaaaactgt
     5641 caaaaaaaga aaaaaagaat aaaattttga cagtttcttt ttttaaaaca gtagtatgat
     5701 aagagtagtt aaagaaagcg tttgcatata atggagggaa tcacaatgac agaatcaacg
     5761 tatatcatgg cgattgacca aggtacaact agctcacgag caattatctt tgacaagaaa
     5821 ggaagacaca tcggcagttc tcaaaaagag ttcacgcaat atttcccgca agaaagttgg
     5881 gtggaacatg atgcaaatga aatctggaat tctgtacagt cagttattgc cggagcgttc
     5941 atcgaatcag ggatcaaacc aaatcagatt gctgggattg gcataacgaa ccagcgtgaa
     6001 acaacagtaa tatgggaaaa agatacagga cgtccaatct atcatgcagt tgtttggcaa
     6061 tccagacagt cagctggaat tgcagataac ttaaaaaatg aaggctatca agaatttttc
     6121 cacgaaaaaa cagggttagt gatcgatgcc tatttctcag caacgaagat acgctggatt
     6181 cttgatcatg tagaaggcgc gcaagaacga gctgaaaaag gcgaattgat gtttggaacg
     6241 atcgattctt ggttagtatg gaaactgacc gatggacaag cacatgttac tgattactcg
     6301 aatgccagtc ggacgatgtt gttcaatatc catcagctag attgggatca ggagatttta
     6361 gatttactaa atattcctcg agtgatgtta ccaaaagtca cttctaactc agaagtttat
     6421 ggttatacgc aaggttatca tttttatgga agcgaagcac caatttcagg gatggcagga
     6481 gaccagcaag ccgcattatt cggacaaatg gcattcgaac caggcatggt caagaatact
     6541 tatggtacag gttcatttat tgtcatgaat accggtgaaa aaccgcaatt atccaaaaat
     6601 aatttattga cgacaattgg ttacggcata aatggaaagg tttactatgc tttagaagga
     6661 agtatttttg tggctggttc ttcgattcaa tggctgagag atggacttca aatgttgcaa
     6721 aaagctagcg attcagaaga cgcagcgaaa cgctctacaa gcgaagatga ggtttatgtt
     6781 gttcctgcat tcgttgggct aggtgctcct tattgggatc aggcagcacg aggggctatg
     6841 tttggcttga cacgtggtac gacaaaagaa gatatcatta aagctacttt acagtcgatt
     6901 gcttatcaag tgagagatat catcgatacg atgcaagatg acactggaat caagattcct
     6961 gtattgaaag tagatggcgg tgcagcaaac aatgaatatc tgatgcaatt ccaaacagat
     7021 atcttgaatg taccgatcca acgagcagaa aacttggaaa caacggcgct aggggcagca
     7081 tttttagcag gattagctgt cggttattgg aaagatacag acgagatccg cgaattttat
     7141 gaagctggca agctatttga agttcaaatg gaggaagagc gccgagaaaa actttacaac
     7201 ggttggaaaa aagcagtaaa agctacccaa gcatttgaat aaggaggaaa tccatatgtt
     7261 ttcaaacaag acaagacaag atagcattca aaaaatgcag caagaagaat tggatctgtt
     7321 gatcatcggt ggcggaatca ctggtgccgg tgtggcagtc cagtcagcag catcaggaat
     7381 caaaacagga ttgatcgaaa tgcaggattt tgcagaaggg acttcctctc gctcgaccaa
     7441 acttgttcat ggcggtattc gttatctgaa gacatttgat gtggaagtag tagctgacac
     7501 agttggtgaa cgtgcagtcg tacaaagtat tgccccacac atcccaaaac cagatccaat
     7561 gcttttgcca atctatgagg gcgaaggagc aacaaccttc aatatgttct cggtcaaagt
     7621 agcaatggac ctttatgaca aactggcaaa tgtgacagga accaaatatg agaattatac
     7681 gctgacacca gaagaagtat tggaaagaga accgtttttg aaaaaagaag ggctaaaagg
     7741 cgcgggtgtc tatctagatt tccgcaacaa tgatgcccgt ttagtgattg ataatatcaa
     7801 aaaggctgcc gaagatgggg cttatctagt aagtaaaatg aaagcggttg gctttttata
     7861 tgatggcgat caaatcgttg gcgtcaaagc tcgtgacctg ctgacagatg aagtgatcga
     7921 aatcaaagca aaattagtga tcaatacgag tggtccttgg gtagataaag tacgaaactt
     7981 gaattttacg cgtccagtct ctcctaaaat gcgtccaact aaagggatcc atttagttgt
     8041 agatgcgaaa aaactgcctg taccgcaacc cacatacttc gatacaggaa agcaagatgg
     8101 gcggatggtt tttgctattc caagagaaaa caagacttac tttggtacga cagatacgga
     8161 ttaccaagga gattttacgg atccgaaagt cacacaagaa gatgtagatt atctattgga
     8221 tgtgatcaat catcgctatc cagaagcaaa tatcacattg aatgatatcg aagcaagctg
     8281 ggcaggactt cgtccactgt tgattggtaa ttctggttct gattacaatg gtggagataa
     8341 tggatcgatt tcagacaaga gcttcaataa agttgtcgat acagtaagtg aatataagga
     8401 aaacaaagtt tctcgtgctg aagtagaaga cgtgttgaac catttggaaa acagccgtga
     8461 tgaaaaagca ccttctacga tttccagagg tagttcttta gaaagagaac cagatggttt
     8521 gctgacttta tctggtggga aaatcaccga ttaccgtaag atggcagaag gagctttgcg
     8581 attgatacgc caattattaa aagaagaata cggaatagag acgaaagaaa tcgactctaa
     8641 aaaatatcag atttcaggtg gaaacttcga tccaactaaa ttagaagaaa cagtgacaga
     8701 attagcaaaa gaaggagtag cagccggttt agaggaagaa gatgctactt atatcgctga
     8761 tttttacggg actaatgccc gacgtatctt tgaattagca aaagaaatgg caccttatcc
     8821 tggtttgagt ctcgctgagt cagctcggtt acgttatggt ttagaagaag aaatggtttt
     8881 ggccccaggc gattatctca ttcgtcgtac aaatcatctg ttgtttgaac gagatcagct
     8941 tgatgagatc aagcaacctg tgatcgatgc aattgctgga tattttgaat ggacagaaga
     9001 ggagaaggcg caacagacta aacgtttaga agcattgatt gcagaatcag atctgcggga
     9061 actaaagggg gagaaataaa tgaacagtga tatgactcaa atcatggggg agtttattgg
     9121 gacactgatc ctagttttgc ttggagatgg tgtgtgtgcg gctgtgaatt taaacaaaag
     9181 taaagcacag gcttctggct ggattgtcat cgcatttggt tggggactcg ctgtaacaat
     9241 ggctgtctat atttctggat tcatgggccc agctcatttg aatcctgcag tctctctagc
     9301 tatggcgatg acaggggcaa ttagttggaa tcttgtcgtt cctttcatca ttgcgcaagt
     9361 tttaggagct tttgcagggg ccattcttgt ttggctttct tatttgccac attggaatgc
     9421 aacgaaagat gaaagcgcga ttttaggaac gtttgcaaca ggtcctgcta tacggaatta
     9481 tccagcaaat gtgatcacag aattaattgg tacatttgta ttagttcttg ggttgctagc
     9541 ttttggacaa aacgaatttg cacctggaac gaacgtattc gctgtaggtg gattgatctt
     9601 agccatcggt ctttcattag gagggccaac aggatatgca atcaaccctg ctcgggactt
     9661 tggtcctcga ttagcacatg cggtattgcc aatagctaat aaaggaactt cagattgggc
     9721 atattcatgg gtaccaatcg caggtccaat gatcggagca attatcgcgg taggtgtcta
     9781 ttcattaatg gtgtaaatct ataaaacata ctaaagtttg acaaccaaaa tgtgtcttgc
     9841 tatagttaag tggaaaataa ataagaccta gaagaatggg gtatgttttg tgagtgtagc
     9901 tggtatctaa tcagtaagtc gaatagccaa taaacaaagt ttatttggtg tgcttatgaa
     9961 acggtacctg tcaggaaaac aatatctttg tattctaata aggataggac atgacttttt
    10021 taagctgtgt tctatccttt tatttatctc taggccctta tttattttgg gacaggtatc
    10081 tcttcaagaa aaggagagaa tatggacaac gaaatgatta gtcaattaca atttgatcgg
    10141 atcaaaaagg aaatccaagc aagagcaatt ggaaactata gcaaaaaacg aatcagcgat
    10201 atgacggtct cgacaaacct acaaactgtc ttggatcgtc aagaagaaac aagagaagca
    10261 cgcttgatct tagaaagcag tcagcatgtt ccatttatgg ggttgccgag gatcgatgca
    10321 ctgacagaac aagtcaaaaa agggttgatt ctgcagccaa ccgatttgat cgaatatgcg
    10381 gattttctta gaagcagtcg gatgatcaca aagttttttg ataagaatca ataccagaca
    10441 cccttgttgt ttgcttatag caaacatctt cctgatttga taaatgtaga agagttgatt
    10501 gaccagaaaa taaaaaataa taaagtcagt gacgacgcgt cacgtaattt gcgtaaagta
    10561 cgcaaacaat tacaactaat cgaaaaagaa atccagtcaa aattactgaa attcttgcgt
    10621 catccgaaaa acaaagaaat gatccaagaa gcaatgattg ttcaaaaagg cgaatactat
    10681 acgattccta tcaaagccag ctataaaaat aaaatagatg gaacgattat cgatgaatca
    10741 aataaaagaa cgacggtttt tattgaacca actgtgattt ctaaattgaa tgaacactat
    10801 cagttgttaa aagctgaaga aatcagtgaa gaatatcaag tcctggcggc attgactggt
    10861 gctatcgcag aaaacgaaga agtgatcgat ttattgatcg aaacgatcac ggtgttagat
    10921 atcatctttg ctcgggcaaa atttagccga gaaatcaatg gaagcacacc tagaatcaat
    10981 aaatcggaac atatcattat caagcaaggc agacatccat ttttacctga gcatgcggta
    11041 ccattggatg ttgaaatcgg taaagactat cgaggcttga ttatcaccgg agcaaatgcc
    11101 ggaggcaaaa cagtcgtgtt gaagacagtc ggtttattga ctttgatggc gatgttcggt
    11161 atgcaagttc cggcaaaaga agggacagag cttgctgtct ttgatgagat tttcgtcgat
    11221 gtcggtgacc accaaaattt agaaaatgcg ttgagtacat tttctggtca tatgcaaaac
    11281 atcgctgcta ttttgaaaaa gataaaaagg aatacgttgg tacttttaga tgagattggc
    11341 agtggaacag agccaaatga aggggctgca ctagcgatcg caatcatgga atctatgtat
    11401 gagcaagggg cattgatcat tgccacgacc cattatggag aaatcaagaa attcgccgga
    11461 gatcatgaag attttgtgcc ggctgctatg gctttcgatc gggaagcact tcgtcctaaa
    11521 tatcagttgc gggttggaga aacaggagaa agccaagctt tgtggattgc tcataaaatg
    11581 gatatgtcga tgaaattgat ccatcaagca gagcgttact tggcggaaaa agcctatcgg
    11641 acacagaaaa aagaatttct tcctaagaca gcagttacaa ataggaagga gaataaaaaa
    11701 gaacgccagc tctttgcaaa aggtgatcgg atatttgtca atgaatacca aaaagaagcg
    11761 ttagtttatg aagatatagg tgaagatacg attgatgtat atttagacaa aaaaataatc
    11821 cacgtgcctc gtcagcgtgt tcgtctggtg cgttcagcag aagatctgta cccgacaggt
    11881 tacgatttag acagtctgtt tatcgattat aaaacacgta aacggcagcg tgacctagag
    11941 cgaggatcaa aaaaagctca taaagtacta gtaaaagaaa tgcgcaaacg acaggaagaa
    12001 agaagagtaa acgatgaaaa cagcaagtga ttatctcaaa ttagctcata atatcagtgg
    12061 ctgtaaagaa aaataaaaac agtgaaatca ttggttttat tccccgtgta agctaaaata
    12121 aaaagaaaag agtgctgagg aggtagggcg ataaaatgaa attaagcaca gactggcgta
    12181 aagaaataca aacgattccg aatcttttat cgatattccg tatttttttg ctgccgattt
    12241 atctttattt tgtgctgaga cagtcatttt atatagcagg agcggtcatt gttgtctcag
    12301 gtttgtctga ttatctagac ggggtcattg ctcggagata taatcaggta acagacttag
    12361 ggaaggtgct tgatccgttt gcagataaat tgacacaact ttttctgatt ttgtctatgg
    12421 catggtatcg accgtggctt tggctgttgt ttggtctatt cttgataaaa gaaggcttta
    12481 tgtttgtagc tggattaatc gggctatcta aaaatatcaa attaagtgga gcgaagtggt
    12541 atggcaaagt agcgactgct gtcatctatg ttggcatgat ccttttgctg cttttcccag
    12601 aattaccaac gctgtgggtg agagtcattt ttgccgtcat cacttacggt cttttacaat
    12661 cttttgtctt gtatgctgtc gagtaccgaa agatgtttca aagaaaataa aaaaggggtg
    12721 tgacctatgt cgcattcctt tttttgataa atagactccc agtagctact tctcgataat
    12781 aagccaacca aaccaaaaac aggagaag
//
LOCUS       DACEPW010000069        12790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEPW010000069 DACEPW010000000
VERSION     DACEPW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.070"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..802)
                     /locus_tag="IW819_001511"
     CDS             complement(83..802)
                     /locus_tag="IW819_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1244622.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(851..2497)
                     /locus_tag="IW819_001512"
     CDS             complement(851..2497)
                     /locus_tag="IW819_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1244623.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2676..3266
                     /locus_tag="IW819_001513"
     CDS             2676..3266
                     /locus_tag="IW819_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1244624.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3358..4863
                     /locus_tag="IW819_001514"
     CDS             3358..4863
                     /locus_tag="IW819_001514"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1244625.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(4947..6299)
                     /gene="celB"
                     /locus_tag="IW819_001515"
     CDS             complement(4947..6299)
                     /gene="celB"
                     /locus_tag="IW819_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1244626.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6299..6817)
                     /locus_tag="IW819_001516"
     CDS             complement(6299..6817)
                     /locus_tag="IW819_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244627.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(6833..7159)
                     /locus_tag="IW819_001517"
     CDS             complement(6833..7159)
                     /locus_tag="IW819_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1244628.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7172..9151)
                     /locus_tag="IW819_001518"
     CDS             complement(7172..9151)
                     /locus_tag="IW819_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1244629.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9172..9486)
                     /locus_tag="IW819_001519"
     CDS             complement(9172..9486)
                     /locus_tag="IW819_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1244630.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9654..9947
                     /locus_tag="IW819_001520"
     CDS             9654..9947
                     /locus_tag="IW819_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1244631.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10025..11137)
                     /locus_tag="IW819_001521"
     CDS             complement(10025..11137)
                     /locus_tag="IW819_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1244632.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11524..11703
                     /locus_tag="IW819_001522"
     CDS             11524..11703
                     /locus_tag="IW819_001522"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244633.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            11897..12067
                     /locus_tag="IW819_001523"
     CDS             11897..12067
                     /locus_tag="IW819_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1244634.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12491..>12790)
                     /locus_tag="IW819_001524"
     CDS             complement(12491..>12790)
                     /locus_tag="IW819_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1244635.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4032 a         2574 c         2166 g         4018 t
ORIGIN      
        1 gccatccgaa agtcagacgg acaagtctgc ttttcagatg gcgctacaac tactctgctt
       61 attcttttag ctatccattc aattattttt ctctcacttg ttctagctct tctttggtca
      121 tgggtcgata ttgcccttta ttcaaatctt taggcagcca aaattttccc atacggattc
      181 tttttaaata taagacttct tttcctactg ctttgaccat tcgcttaact tggtgaaact
      241 ttccttcatg caaaatcaga cggataattg aagtttctgt ctcttgatct acttgatcaa
      301 tttggagttt tgccggcaat gtctgctcat cgccatcaat aatcagtcct tctgcaaata
      361 aacggatatc ttcttctgtc attataccgc taacctcagc tagatattct ttctccacat
      421 gttttttagg tgatagtaac tgatgggcaa acaccccgtc attcgttaaa atcaagaggc
      481 cctctgtgtc tttatctaat cggccgactg gaaaaagatc ttcccgataa tcctggtctt
      541 ccaataaatc gattacggtt tgatcatgat tatcctgagt agcagaaatg actcctgcag
      601 gtttatgcaa aatgtagtaa aaatcttttt gatacgtaat gatttctcct aaataagtaa
      661 ccgcatcttc gaattcattt acttgcgctt tatcgctttt caccacttgt ccattgactt
      721 cgatcgttcc ttttttgatc aatcctttga cttctttgcg gctgccaagt cccatatctg
      781 ctaaaaactt atctaaacgc atgcgtttcc cccatttcaa aagagcatca atctagctct
      841 tctgattcac ttattttatt tttaatttat gtcgtaaacg cgctattcct ggtcctagta
      901 gtctttctgc caagcggatt ttcaaagcta aataaccata cactaaagcc cctatacctg
      961 ctactacaag aatcaacaat agagactgga atttgctatc ttcgtttaaa aaagcaccaa
     1021 aaatcatctt tgtgatccac gaagccaaaa gcataatcaa ggtaagaata aagatcagca
     1081 atgtcctgcg ccagacaaaa cttggtttaa agcgggctac ttcatggatt ctctttaaga
     1141 tcaaggtaca agacacagca aatccgatca ttgtcgccaa taaagggccg tatacttcaa
     1201 ataaacgaat ggctgggtat tgaagaagga gtttcactag aaacccaata accaaataat
     1261 taattgccgc cttgttttca aacattcctt gcagcatgtt ggaaactagc atatatagtc
     1321 ctaggaagag tcctacaaaa cttgcttgga tcagtacatt acttcctaga gaatccggtg
     1381 tataaaataa tgtatttagc ggataggcaa gtaacataac accaaatgtc gccggaaaca
     1441 taaaaaatga aaacaactgt aaatttccgc ttgtcaattt tgccagacct actcgatctt
     1501 tcaacgtaac agcttctgta atcaacggta acccagtaga cgcgatcgat gttgccaatg
     1561 caataactac catcgtcaac ttatctggat tggcactaaa aatcgaaaaa agatccagta
     1621 actgtgcatt ggaatactcg gtggatgcag acatgatctt cataaaagtg aactgatcga
     1681 ctaatttgaa gatcgttacc cctgaaccca caataataaa cggaatcgct tctttaacgg
     1741 tatctaacaa caattccttc gttgcaatat ttattttgtt ttcgctgtag tgaataaacg
     1801 ccgatgtata cgcttgatgt ttcttcaaaa aatacaaaag gacaagaata cttgctagca
     1861 taccgataaa tgccgcgaaa gttgattgcg tgaccgccgt cacatagtca ccatccatga
     1921 ctttcatgat gatgaatgta gccaatagca tataaaaaat acgtgccacc tgttctacaa
     1981 tctgcgacag cgcataaggc atcatttctt gattgccttg aaaataccct cgtatcacac
     2041 tcatacaagg aaaaaccaat acagcgatac tcaaagaacg catcgttgga atcagttctt
     2101 ctccgccacc agaagcatga gctagccaag gagaggcgat gtacatgaac aaagcaaaca
     2161 acaggcctag cccgcccatc atctgtaatg cacggataaa taatcgtcgt gaagtgccat
     2221 attcattcaa tgaattatag cgtgcagttt gttttgcaat cgcagcaggt attcctgctg
     2281 tagagatcag tagaaacaat gcatatatat tgtatcccat attgaataat ccattggcag
     2341 ccttagcatt ttcgcccatc caagcatacc agggaatgat atagacagcg cctagcaatc
     2401 ttgaaatgat attactggcg gtcatccaag cagagcctcg tgccattttt tcttgcggtg
     2461 ttaattgatc taatgctggt tttctttgat tatccatcgg tcagattcca actttctaca
     2521 aaacattact atcattttaa ttggtaaaac agaaactggc aatcgatttt atataacttt
     2581 tataagaaca attgtttttt tgaattcggt ttcgtttttt tggtaaagta aagaaatata
     2641 cagcctttta tggctaacat ttataggaga tatcaatgac acaagaaata aaaaaactac
     2701 ccgttatatt tttaggtctt ttctttatca cagttggatt gaattggttc ttgctccctc
     2761 acgatatagc ttccgccgga gtgggcgcca tcggacattt agttgaaacg gccttttcta
     2821 tccaacgctc agttactgtt tggagcatca atcttatgat gctgcttcta gccgctgtcc
     2881 tattaggaaa atcagtattt tttaagactg tagcgggaag tctgcttttt cctgttgttt
     2941 tagagattgt tccaaaagtg gaaatgatct cctcacattt cttttcactg atttcaggaa
     3001 gtcttttgtt cagtcttggg gtttacacat tgtacacagt cggggcctca aatggcggta
     3061 taacgatacc gccgattatt ttccacaaat tttttcggct tccaatgccg caaggacttt
     3121 tacttaccaa tagtcttatc gtctttttga attattatgt ttttggattg ttggagacac
     3181 tattcgttct cctatctatt acagtcagtt cattgtttat gaaaatactc atccgcttgt
     3241 cccctgtgtc aagaaaattt gaataaagct ggattttgtc atagcggaaa caaacgatat
     3301 attaaaaaga gtgtgctata ataaaccaag aaattgaata aagcgaggga tcaatcaatg
     3361 agcttctctt tagaagacat ccgtcattta ctattaaaag aaaatctatt aaaagaattt
     3421 gtttcaacaa gaggctggca tcttgatgta ccagaaaaag ctgaatttct tcagttaagt
     3481 tatgattcaa gaaaagctga tgcttctact ttgtttttct gtaaaggaat gaatttcaaa
     3541 gaagaatatt tggattcagc aatcgaacag ggtattcaat attatgtatc agaacaacca
     3601 tacgaaaatt cagcagtagg aatcatcgta acggatatcc gtaaagcaat ggcgcttctt
     3661 gctatggctt tttacgacta tccgcaaaat aaattgaaag tgatcggatt cactggaaca
     3721 aaaggtaaaa caactgctgc gtactttaca aaagcaatct tggatcacac aacaaataaa
     3781 aaaacagcat tgctttctac tatgaataca acgttagatg gcaaaacata tttcaaatcc
     3841 catttgacaa cgcctgagtc attagattta tatcgtatga tgtcagaagc agttgaaaat
     3901 ggtatgactc atctagtgat ggaagtttct tcacaagctt acaaaacaca acgcgtatac
     3961 ggcttgactt tagatgtcgg aatcttcttg aatatctcgc ctgaccatat cagtccgatc
     4021 gaacatccga catttgatga ctatttctat tgcaagcgac aattgatctt gaattcaaaa
     4081 acagttgttt taaatcatga aagtgactat tttgatttac ttagggaaac agccgatcta
     4141 ttccagattc caacgatcac ttatggtcgt tcggaagaaa gcaattatca agtgatccgt
     4201 tcagataaag gtactcacgg ttttacttta tcttctcatg aagatcgctt agctatctgc
     4261 gatactgctt atgatatttt gctggctgga ggattcaacc aagaaaatgc agccagtgcg
     4321 atcattgccg cagcactaac aggtgcctcc agtcaagatg cacaagacgg attaaaagaa
     4381 gctcgtgtac caggccgaat ggatcaattg atccaatcca atggagcgca tgtctatgtg
     4441 gactatgcac ataattatct aagcttgaaa acattgctgg aatttgccaa aaatgaacat
     4501 ccagatggcc gtgtaattgt tgtattaggc agcccaggaa acaaagcaat ctcccgtcga
     4561 catgattttg gaaaagtgct atcagaaaca gctgacatcg cctttttgac ggcagatgac
     4621 ccagcatttg aagatccgaa aaaaatcgcg gaagaaatca atgaagcaat tactaatcca
     4681 gatttgatcg ttcattatga aatggatcgt cctgaagcga ttcgacgcgc tttagcagaa
     4741 agcgctccgc aagattcagt cgttattgcg ggaaaaggcg tggatcccta tcaaaaaatc
     4801 aatggtgtag atgaacctta tgaaggggat tatgcaatcg tgaagcggtt gattgaagaa
     4861 taatggtatt tatatactag tggtttccca tttctaagaa aaaaagggac tgtgattttt
     4921 tgtcacagtt ccttttttct tagaaatcat tttaactttt cactagctaa ttctgctgcc
     4981 ttttctcttt cttcatttaa taatttatta tcatagaatt tgataaatgg gaaccagata
     5041 atgaatgcga tgattgcaca aatgactgct aacacagcag ctttccaatc accaccacta
     5101 ccaacaaatg ctccgatccc gactggtgaa ggccacggca tctgtgcaag catcggtttg
     5161 acgatttcca gccgaatggc aaaataagcg agtgtagcag aagccatcgg agctaagaaa
     5221 aacggaaccg ctaaataggg attgtagacg attggcatcc cgaaaatgat tggttcattg
     5281 atattgaata atcctggtac aagtgaggct tttcctaaca ctttcagctg atcagatttc
     5341 gcgaggaatg caataaagat gatcaatcct aaagtcgcac ctgatccacc aacggttaca
     5401 taagaattgt tgaattctcc tgccaacgga taatttgcgc cttgtgcgtt agctgccata
     5461 ttagctaata cgatcggggt caagaaagaa gtgatgatat tcgctccatg gatccctacg
     5521 atccacaatg catggatcaa gaaataaatc accatgacac caagccatgt attcgtcaac
     5581 tgcgtaacaa acccaaatgg gatagcaatc attttgaaga tatccgttcc tagtgcaact
     5641 aataatccat tgatgatcat caccacaaac gcaatgacaa atgttgggat cagagcagtg
     5701 aaagaacgag aaactcctgg cggaacaaca tctggcattt ttatgaccca atttctcttg
     5761 acacacatac aatacaattc gaccgcaata atcgacataa taatagctgt aaagattcct
     5821 gaagttccta aacgttcgac aaaagagccc atcctaatac catttactac agtttctgtc
     5881 tcattgatag aagcaatcaa gctgatttta ccatcagtca tgatcaattc agggatacac
     5941 ataaagaatg caaaaacaga tagtaatgcc cccgtgagcg gattaacatt taatttttgt
     6001 tcatctcgtt caattgacgt taattcataa gcaaacacaa tggcaaagta aatagataaa
     6061 atccccatcg tcattgtatt gacgatcatg tacaagtcac taactttgaa aaaagttgca
     6121 ttaaaaaatc cttctaaaaa tgggaacggt aatggcaaaa tatttaaaac caaaaacatc
     6181 gagcctacaa tcgtgaaagg aatagaagcc atccccgctg ccatgacagc ccgaacgatt
     6241 ttaagttgcg caattttccc cataggtccc atcaggtatt tctgcaaaaa atcaaacatt
     6301 agttattctc ctttcctaaa tataacgctt tttcatattg tttaatacgg atatactgtt
     6361 ggtcttggtt gagtgaaatt ttctttaaac gatactgtat aaatgcactg gcaaaaatac
     6421 cgcttacaac aagaatgaaa acaaataact gtcctccatt gttatttgcc ataaaaggat
     6481 acaagaatgt aaatcctgag gtaaacaaca ctggaatcac taataaatta aagacactct
     6541 ggaacaaaaa ataccacttt gtccatggaa cgttcgtttg gtgttttcga taaagcttta
     6601 cttgttctcc tactgcaggc aaaatagcta aaatcagaat catcggtaaa ctacttccta
     6661 aacttttagc tgatagaagc agaatcatcc attgcatgtt catgaaaaag aaaaaggcag
     6721 tcaggtaacg tacgattaga taacgattga aatacatgtt tttcaaacta agttgctttt
     6781 tttctaattc gattttcctt tttactgtat cctgcacaat gatccttcct ttctatttat
     6841 ttttttgata caaataattc atttcctgtg caacttccaa taatgtcatc gtcgtcatca
     6901 gatgatcttg tgcatggacc atgataattt ccatttctat ttttgtcccg ctggcatatt
     6961 ctttcagaag ttgagtttgt gactgatgcg catgaagaat ctcttcattt gcttgtcgca
     7021 acttctcttc tacttcttca aacatctctt tacgcatcga gcgaaatgct tcatgtatca
     7081 gtgtccgtgc atttccgcta tggaaaatga tatcaaaaga agtcacttgt agtgtttcac
     7141 tgtttatttt ttcagccatc tgttttcctc cttattcgat cagtttgata aatttactgc
     7201 tgaaattctc aaaaataggt tcagctaaga tttcctgctg tacttttagc tggtcaacta
     7261 acctaacgat tgctttagtc acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa
     7321 ctaaaaaaac aaaatgaatt tcttgatatt gatcccactg catcccagaa ggaatcaatg
     7381 caacggctac ctttgtttcg attccaactg gaacagcggg atgtgggact gccactgtat
     7441 cactgaatat gatttgtccc atattttcac gttgctggat ctgctttcgc atttctctta
     7501 tatatccttc ttcttcattt atgctcagct tttgcagcaa atcatgaaga atttcttcct
     7561 ttttcggttc gtacgtataa gtcttgaaaa atttttcaga caactgttcg gtgtaaatct
     7621 gctttttttg ctgtagagaa aatgctggta tcggtctatc tgaggaatga tagggtatag
     7681 attctccaac tacctttcgt attttacgaa catcatcttc attcaaaaaa atactgacat
     7741 gtaaaactgg aattttgaaa aacattgtcg aaagatcaat agaagaaata atcaggtcga
     7801 ttccttttaa agttgtttca ttgatctcat aatagccttt tacacttacc actgtaatat
     7861 ttttaccgaa ttcactgact acgcgattct ttaatagttg ggcactgccg taacccgttg
     7921 cacagataat caatgcatgt agtttatgcg cagctctttc tttttccaat gccgccatga
     7981 gatgcaaagc caaataggcc cattcatctt cattgatggt atagtttttc aaagccggca
     8041 tgttgctgaa atgccttttt actgcgtcaa agactttggg attttctttc tttatctcct
     8101 cagtcaatgg gttctcaatg cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat
     8161 gatcgagtag tccattgaaa agctggagat cctctactaa tgaaaaatga aattcctgag
     8221 acaatctttt cagaactttc gctaattcct ttgatagttc ctgatcattt tgattttctt
     8281 tgtgattcga cttagccatg agatgtaatg tcaaataagc aatttcttct tcgggaaacg
     8341 atacacctgc gattacctca atacgttgaa cgatccgctg cgccacttga tactctactc
     8401 gcttagaaac ttcttgttcg atgcctaatt ccttgatttc aaacccttct cggagccgtt
     8461 tgatccccaa agccaagtgt aaagtcagat tctgtatgat aaaatccgaa acttttaact
     8521 tagcttctct tgtttcatcg agaatgataa tgactagctc ttcaaaactg atgtctggga
     8581 aaaaacggct ttgccctaaa tattctttca atgaatttcc ataatggttt ccaaaaaaag
     8641 tatccatgat aaaatgccgt cgatttcttt cttccccttc gatccatgtc ccttgaccat
     8701 gcttagaaac gatttttagt gaatatggaa tcaatcgctc cttgatttct tgcatgtcct
     8761 ttgtcaaact ggaacgactg atataaagct cttctgccaa atcatccata aaaagaatgg
     8821 catcttccaa aagcaattgg ttcaatatat acttttgtcg atcttcggac tcataaaatc
     8881 cttgcggttc tctagcatat ccaggtacta tttcttgccg actcaaaaac aagtcaaatg
     8941 tcagtctttg taaaatatgc agttggtagc cataacctgg tttagcaatg atcttacctc
     9001 cgtttttttc tactaactct ttgatccgct gcaagtaatt acggattgtc cgatcagata
     9061 ataacaattc agaagctaaa cgttgactgg tgacatactc cttcttgtga tcaatcaaat
     9121 atctcaacag caatttttcc ttctgtttca ttatcatcca ctcctattcc atcatatgtt
     9181 ctcttcgatc acctctgcta acttctggat gcctgtaggg attgggacat acgcttggaa
     9241 agggatacta acaacaggct ttccaacaga ttctcctaat tttttgaatt tatccaaaaa
     9301 catcgtcgtt tgcgggctga tcagaaataa atcaaaatca ctattcttaa tcatattatc
     9361 gccttctgta gctgaaactg catctacgag tacctcttct cttttttgct taaagaaatc
     9421 cgttgtcttt gaggccatca tcgaggatga cattcctgcc gcacaaataa tcaatgcttt
     9481 tttcataaat caatcactcc ttttattttt ataaaccaag catatcttgt aagcgttttc
     9541 tattcaaaat atatatttcc gtagcattgc ggaaaaaatg tgtatcatat cttgaataat
     9601 aatttctggc accgtgatat actagtgata gaattttttc aggaggggat ttagtgagta
     9661 tcacagtgaa tattttgtat acaagtacaa atggacaagc cagaaaattt gcagaagaaa
     9721 tggtcgaaag aaacattgtc aaagcgattc gtgcagaaga aggaaacgag aaatatgact
     9781 attactttcc actagaagat ccagaatctc ttttactgat cgaccgttgg aaagatgaag
     9841 aagcaatcga aaagcatcac aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa
     9901 agtataaatt aaaaatgaaa gtcgaacgtt tcactgattt accgtaaaaa gatacacatg
     9961 gaatgatagc aatttatcat ccatgtgtat cttcactttt tccttatttt ttattcgatc
    10021 tcatctaatg attcaaaccg tgtttttctc ttatttttcg gataagtatt tcagtcaccc
    10081 caatatacat gattcctaaa cataccccaa ggatattctc aaaaatgcgg atcgttactg
    10141 ctcctggaac cccgaataaa ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg
    10201 ttttatatct ataggtagaa caaacaccta aagctagacc acctaatata ccgacccagt
    10261 taaaaggaat aaattggctg ataccaataa ataaaacaca tccaatcaat gagccaatga
    10321 ttcggtctac tgctcgttct ttgaaaccaa tcgacatcaa tccatagcta gatacaatag
    10381 aagaaaatgc aaaccctgcc cacatcaatc gctgaaaagg gacatactct cctgcaacga
    10441 tcaaaagact aatgccaaat gcatagatga gcatccaaat tttttcctgc gaatacatgc
    10501 ctttcaaaaa aatctcttta aacaagcttt tgtctttgtt cttttctcga tgttttctgt
    10561 aaagaagaac tgaaaaccaa caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga
    10621 aattcttgtt caacgaatct ttaggtaacg aataaacaat aaagagatat gaaaagccgt
    10681 acaaagaagc attccccatt ttgggatcac tagcggttgc cattaatatg ctgcttagta
    10741 aaataaaatg aatcaacatg cccataactg accatttgat cagctggaca tacggtgcta
    10801 ctaatagtga gaacatgacg attgctaaac ctatgatcga atgggaaatc ttataaccaa
    10861 agtcaacaaa acgaatactc atcaacatac agaataaaac cacagcgtat ggtttatgct
    10921 tcgattcaaa taatgttgta attataatga taaaacaaat ggcaaacaat acgatgaata
    10981 atgatcgtaa aaacaatgaa caaatataca tgcacttttc ttttttagaa gctgcttctc
    11041 gtattttttg ttttaaaata aacggatcta gttgtagaga ctgataaaaa tccacctgtt
    11101 gtcgtccttt ctttttactt ttatttttta cagtcatcta tgacaggttg taaatactat
    11161 ggaacatcat atggcgttgt cattttcttg tcaactattt ttcctattca tgtttttata
    11221 aaaaaacata tcgttttctt gtatgattaa atcaccacaa aataaacaac tgagagaacg
    11281 atatgcttta tgaatggttc tatcaaaaaa attctgagat caatagacaa aaatttgatg
    11341 attacagagg tctcttacga aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt
    11401 ctcttccctg ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg
    11461 aaattaatgg tggtgaaaaa caagaagaag gaaaccattg tccactttga ataatataat
    11521 catatgccaa tggttatacg cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc
    11581 cattggaacg ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga
    11641 cataaaatca cttctttact gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt
    11701 taggtatctt tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta tttaccaaag
    11761 tcttccaaaa gaattctgtc ttgagtgttg agggttgtga atttcgttct cacgtttccg
    11821 ttgaggataa actgagtttt atttccgcgg atggcgagac agaaaaatta atcgagaaaa
    11881 tttacctaca caaaaaattc ctcgattaac aaactatttc cttagctgca ctaatcctaa
    11941 agaaatagaa ttcttagtta cagacatgaa cgccacctac tttcagctca ccaaacatgt
    12001 tctaccaaat gcgaaattgg tgattgatcg attacttggt ttttccacgc ctttcaagga
    12061 ggcgtaacct ttattcacga gttggttgaa gctttccgag acaaaagacc cgacttgttt
    12121 ttctccttat tgacaaagag ccattaaaac tagagaaaaa attacaactg atcgctgatt
    12181 atttgtagaa agaaaatcaa agtattaccc aataaacttg agtcattagg caaaaaacac
    12241 tgcgaaactc aagaatgatt ttcgcagtgt ttttgtatat tttttattaa ctagtattac
    12301 tacaaaggtt tatttttcac ttctcaacgc agcacaaaat tgtctttact tatttactgg
    12361 ttgggttcaa ccttctctac tccgatacta atatttgttt gtatacccct tctaaaatcg
    12421 gtagtttcca ttcaattgat gggtactagt tgattttaaa aactatgctt attcaatttt
    12481 ttattttagc tcatatattt cgtttctttc taaattttat actgatgaaa acaagtagca
    12541 cacccaatat tgacaatata aactggctaa tgatggtttc tccagttttt ggaagatgct
    12601 tatttccact gcttgttgga ttgtttcctc ttatatctga gctatcagca acttttctat
    12661 tctcttttgt taaaaagatc gccacttctt gttctttttc gatcttgaaa gttactggtg
    12721 tcgcatccaa ttcatacccc gttggcgctt gagtttctac cagctgatat gctcctggtg
    12781 ctaagccatc
//
LOCUS       DACEPW010000070        12668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEPW010000070 DACEPW010000000
VERSION     DACEPW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.071"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1863
                     /gene="ligA"
                     /locus_tag="IW819_001525"
     CDS             <1..1863
                     /gene="ligA"
                     /locus_tag="IW819_001525"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1244636.1"
                     /translation="ITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            1969..2274
                     /gene="gatC"
                     /locus_tag="IW819_001526"
     CDS             1969..2274
                     /gene="gatC"
                     /locus_tag="IW819_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1244637.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            2274..3746
                     /gene="gatA"
                     /locus_tag="IW819_001527"
     CDS             2274..3746
                     /gene="gatA"
                     /locus_tag="IW819_001527"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1244638.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            3743..5173
                     /gene="gatB"
                     /locus_tag="IW819_001528"
     CDS             3743..5173
                     /gene="gatB"
                     /locus_tag="IW819_001528"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1244639.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            5183..6238
                     /locus_tag="IW819_001529"
     CDS             5183..6238
                     /locus_tag="IW819_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1244640.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            6349..7722
                     /gene="rlmD"
                     /locus_tag="IW819_001530"
     CDS             6349..7722
                     /gene="rlmD"
                     /locus_tag="IW819_001530"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1244641.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            7725..7853
                     /locus_tag="IW819_001531"
     CDS             7725..7853
                     /locus_tag="IW819_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1244642.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            8293..8481
                     /locus_tag="IW819_001532"
     CDS             8293..8481
                     /locus_tag="IW819_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1244643.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            8559..9572
                     /locus_tag="IW819_001533"
     CDS             8559..9572
                     /locus_tag="IW819_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1244644.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(9639..10673)
                     /locus_tag="IW819_001534"
     CDS             complement(9639..10673)
                     /locus_tag="IW819_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1244645.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            10788..>12668
                     /locus_tag="IW819_001535"
     CDS             10788..>12668
                     /locus_tag="IW819_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1244646.1"
                     /translation="MDMTHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEE"
BASE COUNT         4238 a         2302 c         2735 g         3393 t
ORIGIN      
        1 atcacgtctg attctcccac tcaacgagta ggcggaaaag tattagaagg ttttgaaaaa
       61 gtcactcatg atattccgct ttacagtttg aacgacgttt tcagcaaaga agagctgata
      121 gcttttgatc aacgtgtaca aaaagcagtg ggacgtgtag ttgactattg ctgtgaacta
      181 aaaatagatg gactttccgt ttctttgcgt tatgaagatg gaaatttcgt gagaggtgcg
      241 actcgcgggg atggaactgt aggagaaaac atcactgaaa acctaaaaac tgtccggtcg
      301 gtcccaatca aattaaaaga accaatgaac atcgaagtaa gaggcgaatg tttcatgcca
      361 aaacggtcat ttgtccaatt gaaccaagac agagaagcag agggaaaaga tatctttgcg
      421 aatcctcgga acgccgcagc tggttctttg cgtcagttag attctaaaat cacagcgaaa
      481 agaaatctgg acactttttt atacacagta gctgatttcg gtcctatgca agcaaaaacc
      541 cagtatgatg cgctagaaga gctagaaaaa attggattcc acacgaatcg ggaaaaacgt
      601 ctatgtcatt cgattgatga agtgtggtca tacattgaag aatatcatga caaacgtgtg
      661 gatttgccct atgaaatcga tggaatcgtc atcaaagtga atgaattctc gctacaagat
      721 caacttggtt ttactatcaa agcaccgaga tgggcagcag cttacaagtt tccgccagaa
      781 gaagttgaaa cgctaatcga aaacatcgaa tggacagtcg gtcgaacagg ggtagtaacg
      841 ccaacagcta tcatgacacc agtccgagta gcaggaacga cagtaagccg tgccagcctg
      901 cataacggtg attatattaa gttgaaggat atccgcttga aagatacagt tctgatttat
      961 aaagcgggag acatcatccc agaagtttcg caagtcgtgt tagataaacg acctaaagac
     1021 agcgaagaat accaattgcc tacacattgt ccagtttgtg gaagtgaact tgtccatttg
     1081 gatgaagaag tagccttacg gtgcatcaat cctaaatgcc ctgctcaaat gaaagaaggt
     1141 ttgaaccact ttgtttcaag aaatgcgatg aatatcgatg ggttggggcc gcgcgtacta
     1201 gaacagatgt atgataaaaa actagtagca gatgttgctg atctttacaa attgacagaa
     1261 gaagagttat tgacgttgga caaaataaaa gaaaaatcag caaataatat tttgacagca
     1321 atcgacaaca gcaaagataa ttcggtcgag cggttgattt tcgggttagg aatccgacat
     1381 gtcggagcga aagcagcaaa aattttagca gaacatttcg gtgatctaga aacattgagt
     1441 aaaagtgact atgagtcgat tattgcgctt gatacgatcg gtgatatcat tgccgacagt
     1501 gtagtcactt atttcagtaa cgaagaagtc catgaattga tgaatgaatt aaaacaagca
     1561 ggcgtcaatt tcgaatacaa agggcttcga aatgcccaat tgcaagaagt cgagtctcct
     1621 tttaaagaaa aaacagtagt ccttactggt aaactaacac gatttactcg tgaagaagca
     1681 aaagagacga tcgaaaatct tggcgggaaa gtgactggta gcgtatcgaa aaaaacggat
     1741 atcgtggtag caggagaaga tgctggcagc aagctgacca aagcacaaga attaggaatc
     1801 gaagtttgga cagaagatca aatggcagat gcattggcta aaagtcgctc agtagaagaa
     1861 tgaaaaatga gttttttcag acaaatttca ataaacaatt tcagacacgg cactcttgtg
     1921 ttaaaataga gagcaaagtt tttaactcgt ttagaaagaa ggggtagtat ggcgattagt
     1981 gaagaacaag tgaaacacgt tgccaaatta gcaaaacttg cgttcgcaga tgaagaactt
     2041 gcaagtttta ccgaccaatt aggtaaaatc atcgatatgg ttgaaatgct ggaagaagta
     2101 gatacggaag gtgttccatt tacgtcgaat gtggttgaaa cagtaaacgt gatgcgacaa
     2161 gatcgggcgc aagctggatg gaaccgcgat gaattattga aaaatgtgcc ggaaacagaa
     2221 gacggcttta tcaaagtacc cgcaatcatc gataatgggg aggctggcgc ataatgacag
     2281 aattacacaa tcgttctcta gaagaactgc atcatatgct tgtatcgaaa gaaatcactg
     2341 tacaagattt gacaaaaaca acatttgaac gaatcaaaga aacagagcct gaggtcgagg
     2401 cattcattac attgaacgaa gaaaaagcat tggaacaggc aaaagcttta gatgaaaaag
     2461 ggattgatga aagcaacgtc cttgctggta ttccaattgg tatcaaggat aatatcgtaa
     2521 caaaagatat cttgacaacc gctgcatcta agatccttta caattttgaa ccaatttatg
     2581 atgcaacggt catggataaa atctatcaag cagatatgat ccctgttggt aagctaaaca
     2641 tggatgaatt tgctatgggt ggaagtacag aaacttctta tttcaagaaa acaaaaaatg
     2701 cttgggaccc aacaaaagta cctggtggct cttctggtgg ttctgcagca gcagttgcag
     2761 ctggacaaat tccagtttct ttaggtagtg atactggggg aagtatccgc caaccagcag
     2821 cttttaacgg catcgtaggg atgaaaccta cttatggccg tgtctcacgt tttggtttga
     2881 ttgcctttgc ttcatctctt gaccaaatcg gaccaatgac ccgtacggtg aaagataatg
     2941 cattagcatt gacagctatc agcggttatg acgaaaaaga cggtacttct tcaggcgttt
     3001 cagttcctga ttttgcagag ggactgacag gcgacatcaa aggaatgaag attgctttac
     3061 ctaaagaata cttaggcgaa ggtgtggccc ctggcgtaaa agaagctgta ttgaaagcag
     3121 cagaaacatt ccgttcatta ggtgctgttg tggaagaagt cagcttgcct cattcaaaat
     3181 acggtgtggc tgtttattat atcattgcct catcagaagc aagttccaat ttgcaacgat
     3241 tcgatggtat ccgctacggt tatcgctctg aaaatgtgaa atcattagat gatgtatatg
     3301 tcaactcacg ttcagaagga ttcggtgaag aagtaaaacg ccgtatcatg ttaggtacat
     3361 tctccttaag cgcgggttat tatgacgctt acttcaaaaa agcaggacaa gtccgtacat
     3421 tgatcaaacg agattttgaa aaagtctttg cagattatga cttgatcatc gggccatctt
     3481 caccaacagt tgcattcgga atcggtgaga acattaacga tccaatcaca atgtacatga
     3541 gtgacatctt aacgatccct gtcaacttgg ctggacttcc aggtatgtct gtaccggcag
     3601 gtttatcaga aggattacca gttggattgc agatcatcgg taattatttt gatgaaaaaa
     3661 cgatgtatca agcggcatat gcgtttgaac aagcaacaga attccataaa caacaaccag
     3721 ctattttagg agggaaagac gaatgaattt tgaaacagtc attggactag aagtccacgt
     3781 cgagttaaaa accaactcaa aaattttctc tccagcgcca gctcattttg gtgcagaacc
     3841 aaacagcaat acaaacgtga tcgactgggg ttatccaggc gtgctgccag taatgaataa
     3901 agctgcgctg gaattcggga tgaaagcagc tttggcatta aactgtgaaa tctctaaaga
     3961 tactcatttt gaccggaaaa attactttta cccagataat ccaaaagcat atcaaatttc
     4021 ccaatttgat caaccaatcg gtcatgacgg atggatcgaa atcgaagtag aaggtaaaaa
     4081 gaaaaaaatc cggatcgaac gtgtccattt agaagaagat gctggaaaaa acatgcatgg
     4141 tattggcggt tattcttacg ttgatttgaa ccgacaaggc acgcctttga tcgaaatcgt
     4201 ttctgaagct gatatgcgtt caccagaaga agcatacgct tatctagaag cattgcgatc
     4261 aattatccaa tttacagaag tcagcgatgt gaaaatggaa gaaggctcta tgcgctgtga
     4321 tgccaatatt tctcttcgcc cttatggaca agaagagttc ggtacgaaag cagaattgaa
     4381 aaacttgaac tctttatctt tcgtgaaaaa aggtttagct tttgaagaaa aacgtcaagc
     4441 aaaagttctt ctttcaggag gagaaatcca acaagaaaca cgccgttttg acgaaacaac
     4501 gaataaaaca gttttgatgc gtgtcaaaga agggtctagt gattatcgtt acttcccaga
     4561 gcctgatatc ccgaaattcg tgatcgatga cgaatggatt caaaaagtaa aagctagttt
     4621 gcctgagatg ccagcttctc gtcgcgaacg ctatatccgt gagtttggac ttccagagta
     4681 tgacgcaatg gtattgacac ttacaaaaga aatgtctgat ttttttgaag ctgttttgga
     4741 aaatggtgca gatgccaagc aagcatctaa ctggttgatg ggtgaagttt ctgcttactt
     4801 gaacagtgag aaactggaat tagctgagac aaaattgaca cctgagaact tagcaggaat
     4861 gattcgtttg atcgccgatg gaacgatcag ctcaaaaatc gccaaaaaag tcttccgtga
     4921 attgatcaca aatggcggag aagcccaaga agtagttgaa aaaaatggtt gggtgcaatt
     4981 atctgatccg aacaaactac tgccaatcat taatgaaatt ttggataaca accaacaatc
     5041 tgtcgatgat ttcaaaaatg gaaaagatcg tgcggttggc ttcctagtcg gtcaaatcat
     5101 gaaagcaact aaaggacaag caaatcctgg tgtcgtgaat aagctactta aagatgaatt
     5161 agcgaaacga tagaggctga aaatgaaaaa agcacgcgta atttataacc ccacctcggg
     5221 aaaagaatta ttgaaaaaaa atttggcgga tatcctacag gcattagaag aagcaggatt
     5281 tgaagccagc gcctatgcaa ctacaccaga accagattca gccaagaatg aagcatgtag
     5341 agtcgcagaa ctaggttttg atctagtagt ggcagctggt ggcgatggca caatcaacga
     5401 agtggttaat ggaatcgctc cgcttgaaca ccgtcctaaa atggccatca tcccagctgg
     5461 tacaacgaac gactatgctc gagcattaaa aattcctaga gataatatta aagcagcagc
     5521 agaagtgata aaaaaacacc aaaccgtaaa aatggacatc ggcctttctg ataacagtta
     5581 cttcatcaat atcgctgcag gtggctattt gacagaattg acttatgaag ttccatcaga
     5641 actaaagagt atttttggtt atcttgctta tcttgcgaaa ggagcagaga tgctccctcg
     5701 agtaaagcca atcaaaatgc gcttgaagta tgatgaaggg gaatatatcg gaaatgcttc
     5761 gatgtttttc ctagggctga cgaactctgt tggaggattc gaacagatcg cgccagatgc
     5821 caaattggat gatgggaagt tttctttaat cattgtgaag acagcaaata tctttgagat
     5881 cttgcatatt gcagcactca tgctgaatgg cggtcgacat gtcgatgatc caagaataat
     5941 ctataccaaa acaagcagtt tgttcgttga aacagaagat gatatcaatc gaccagtaat
     6001 gatcaactta gatggtgaat acggcgggga agcaccgatg catttccaaa atcttcatca
     6061 acacattgag ttctttgcca ataccgatca gattcctgat gaagcaatca caggtacaga
     6121 tgaagaagaa ttagaggttg tcagcaaaga attcgtaaaa gaagtcgaac gtttaacgga
     6181 tgaagatatc gacggtgacg gaaaaatagc agacgaaaca cctaatcaaa agaattaatc
     6241 aaaagccggt gctgaacgta gtaaggtcag tgccggtttt agttgtgtaa ttagctgatt
     6301 tatagtagag ttcagtagga ttgaaaaata ggcttaaagg agcaaaatat gacagaacag
     6361 caaatagtaa agaaaaacga acgatataca gtcgaaatcg tcgacttgag ttatgaagga
     6421 atgggggtag caaaaatcga tggttatcca ttatttatcg aaaatgcact ccctggagaa
     6481 gaagtagaaa tactagtagt aaaagcaggc aataagtttg gatatggaaa agtcgaaaca
     6541 tttattacac aatcacctga tcgtcagcct gtagaaaaca atgcattact tcgcataggt
     6601 atcgcacctt tagcccattt gaagtatgaa caacagcttg tcttcaaaca aaaacaagta
     6661 gagcatgtaa tggcgaagat tgctaagatg ccagaagttc ctgtacttcc aacggtcgga
     6721 atggcagaac cattcggcta ccgtaataaa gcacagatcc ctgttcgccg tatcgatgga
     6781 cagttagcca ccggttttta caaaaagaat agccatgatc tagtccctat cgaagatttt
     6841 tatatccaag atcctgtcat cgatcaagca atcaaagtag tcagagatat cttgcaacgg
     6901 ttccaagtac gaggatataa tgaagaaaaa aatgaaggac agcttcgtca catcattatc
     6961 cgtcgtggtc attattccca tgaaatgatg gtcgttttag ttactcgtaa agaaaaattc
     7021 tttaaatcca aggaaattgc tgaagctatt catgaagaat tgccagaagt ggtttcggtc
     7081 atccaaaata tcaatgaaga aaaaacaaat gtgattttag gagacaaaga aaaagtttta
     7141 tatggcagag actatatcga agatcagcta ttaggaaaaa cctatcgaat ctcagcaaag
     7201 tcattttatc aagtaaatac tcaacaagca gagtatctgt accaaacagc catcgagttt
     7261 gccgagttag ataaaaaaga cattgtgatc gacgcttatt caggaatagg tacgattggc
     7321 ttgtctttag cagacagagt ggataaggtg tatgggatgg aagttattcc agaagcaatt
     7381 gaagatgcac agttcaatgc tttaaataat aaaatcgaaa atgttcatta tgaagtcgga
     7441 aaagcagaaa aagttatgaa aaaatggcaa gaaaaaggga tcaaaccatc tgtgatcgta
     7501 gttgaccctc cacgaaaagg attagatcat cattttattg aatcagcaat cgaaatgtca
     7561 ccagaacgaa tcgtctatat ctcatgtaat ccggcaactt ttgcaagaga tgctaagtta
     7621 tttgcagaag caggttatgc cgtgaagaaa gttcagcctg tagatctttt cccgcaaacc
     7681 catcatatcg aattagtcgc ttcattcgtt aaaggagaat aaaaatggac caaaaagaaa
     7741 aaatgaaaga attgatcaaa aagaaacaag gtggaggccg atccaaacaa atggaaacac
     7801 ctaagaatga tcgtaaaaac atgagaaaag gccctaaaat ctttaataaa tagacgtgct
     7861 aaagaaagac taagaagtgt taacttttgt tattgtttct tagtcttttt agttagtcta
     7921 cattattttg gcgatttgcc aataaggact gttgagttgt gcaaaatcaa atctgggtca
     7981 gaatgaacgt cattctgtct cagatttttc gttatttcaa tttgattaac tgattgatca
     8041 aaaagatttt tattttcata ttctcaaatg attcaacact aaatatacat gtttgacgcg
     8101 gaaattatta ttcgattttg gcggcatcag gggggatgac gacgtagcag acccattatt
     8161 ttactcaaga aaatgtccgt gcctattatt tagaatacga tagtgaacgt gctgggagtt
     8221 ttgttctttt agagaaagtc tctgaagata aattagttgt cttagatttt gactgtaacg
     8281 aaaaatggcg aaatggaaga gcaccagcaa gtgattgacc ggatctacga agcgacacga
     8341 tatctgcctt tggatcgttt gtgcttaagt cctcaatgtg gatttgcttc aacagaagaa
     8401 gaaaatgttt tgtttgaaga acaatggaaa aaatttcttt tgtcaaaagc attgcagaag
     8461 aagtttgggg aaaatcatta aatcatctag gagagcttga taggaaataa cactatggta
     8521 taatcagaat gcttaatact aactactgag ggagccttat gaaaaagaaa attcttaaat
     8581 ttatcggatg gttcttcgga atcattcttg ggatcgcgtt tgcaatcttt cttgccttcc
     8641 agctttcccc cagacctgga gcattcttga tcaaccatat gttttcaaat actgttcaaa
     8701 tcactgataa aaaaacgttc gatcaagcag aaaaaaacgt ggcgaaaaaa acagatttga
     8761 cgtatgagtc caagtataaa gagaatactt atgacatcta ctatccaaaa aacagcaagg
     8821 ggcctgttcc tgttctattt tgggtacatg gtggaggatt cgtcggcgga gataaatcgg
     8881 gtgtcaaaga atttgcgaca aaattagttt cagacgccaa catcgcagtg gttgcgatga
     8941 attatgaatt agcgcctgat tcagaatacc ctaatcaagt tcttcaagtg aatgaactga
     9001 tcaaagattt attagctgaa gaaaaagacg acaaattgct agacatggaa caattatttt
     9061 ttggtggaga tagtgcaggt tcacaaatcg cattgcaata tgcagctata caaacgaatg
     9121 atgcgtatgc taaacaaatg aaaatcaaac atctacttcc aaaagatagt ctgaaaggaa
     9181 ccatttctta ttgcggacct gttgatttga agctaacagc acagcagcat tcggatgatc
     9241 gatttatgaa gtttttcgtc aaaaccgttg cgtggtcgtt gattggaaca aaagattgga
     9301 aaacgagtga gcaattgaaa gaagctagtg tggctgacca tgtttctaag gaatttccgc
     9361 caacgtatat aactgacgga aatgcatact cattccagga gcaaggtcaa gcattagaaa
     9421 atcggttgac agaattaaag gtacccgtgc aatccttgtt ttatacagat acaaaaaaag
     9481 agattaccca tgaataccaa tttgattaca cgctaaaaga atcgcaaaat tgttaccagc
     9541 agacattgga ttttgtgaat aagtataagt aagaggttgt gaaaaagccg tttcgttttg
     9601 agtatgaaga aacgaagcgg cttttcacaa cctctctttc aatacctttt tgttcttggg
     9661 ataaaacttt ccctaacaat caatcgtgca ggataagtca aaatgattgg ttcgtcccgt
     9721 ataccgtcaa ttcgctccaa tgccattcga acagctgctt gcccaattga tttcgcattg
     9781 atttgaacac tggtcaagcc aggggagagg tttgcgattg tctcgatatt atcaaagctt
     9841 gctacaacaa tctcttctcc aatttggata tttttttgtt tcaatgcccg atagacacca
     9901 attgccattg gatcgctccc gacaagaatg gcagtaggaa gaggggattc attttctaat
     9961 agattagtcg caagtacctc accttgtttt tctgtccagc cacctagatg ataagacata
    10021 tatttttcta agtgatgatc gatcataaaa tgctgatagg cacgcatcct cttttcattt
    10081 atagaaatct gcttcccacc gaattcatcc atatcgactt tatatccccc gatataggct
    10141 attcttttat gaccattttc taagaacaat tccagtacgg ttttagtcat ccgttcaaga
    10201 tctgcataca ccatatcgac ctcttgctgg atgtcagggt tatccacgac gactatttgc
    10261 atgttttgtt ttaacaaggc ggtaatcgat tctttcgtaa cagtcccaac aacgatcacc
    10321 gcacctaact gatctagatc ttcccattgt ttaggattat cagatagatt atagattcga
    10381 ttcatcccta aatgcagacg tcgagcttct tcttccatcc ctactcgcaa atgtttgtaa
    10441 tagatatcct gttcttcgct cgctttgccg atcgtagaga tcacaccaat cgaaggcatt
    10501 ctctttttga tatatttacg agtactgatg tcataatcca acgcagcagc tgcctctact
    10561 atccgttgcc gagtagtctg agccacatgg atcgtctgat cgttattcaa cacacgggaa
    10621 actgtagcag gagagacatt tgcctttcgt gcgacatctc taattccaac catttttaca
    10681 cactcctcaa aaaaataaaa aatttttatt gacaaaattg tttagtaaac agtatacagg
    10741 aattataaaa aaaagtaaac ggtttccgtt ttgttgggag tgaaatgatg gatatgacgc
    10801 atagaaaaac agataaaaat ttcatcttat ttgatgaaaa gagccgagtc ttccatttac
    10861 gaaatacatt tctttcttat ctgataaaga tagaagaaag caatgtattg gcgcatattt
    10921 attttggcaa accagtcaag cagtacaaag acaataaaaa ttatccccga agagatcgtg
    10981 gattttcagg aaatgttccc ttaaatcctg accggtctct gtcgaaagat acactaccgc
    11041 aagaatactc ttctcatggc agcatggact ttcgaacgcc cgcatcgatc attcagcgca
    11101 agaatggatc agacttactg gatctgagat atgacagcca ttatattaca gatgggaagc
    11161 cagagatcga aggcttgcct caaacttatg tgatggacaa gtcagaggca caaacgttgg
    11221 tgatttcact taaggataga gaaacagcga tttatttcga tttgttctat accatattta
    11281 ctgatagagc agtgatcaca cgatctgtaa agataagaaa tgaaaccgga gaaacaatca
    11341 aactagaaaa agcggctagt ttccagctag actttgccca tactaggcga tttgatgaag
    11401 tcattgcttt gccaggagca catgtcaatg aaagacagat ttcacggcaa tctgttttat
    11461 ctggaacaaa agtatttgaa agccgtcgag gaacgagcag tcaccatatg aataacttta
    11521 ttgctttagt tcatcatcat acgacagaaa atactggaga agcgatcgga cttcaatttg
    11581 tttattccgg caaccattct ttcgaacttg aaaaagatca gatcaatcaa ttgagagtcg
    11641 tcggtgggat caacagccat cgtttttcgt gggaattgaa tgcaggccag tcttttcaaa
    11701 cacctgaaat gatcctttct tactcaagtc agggattgaa caaaatgagt cagatccacc
    11761 atgagttgct acgtgaaaga atcgcaagag gtcgtcatca atttgctgaa cggccgattc
    11821 tggtcaataa ctgggaagcc acttactttg acttcaatag tgaaaaaatc aaggcgatca
    11881 ttgatgaagc aaaggagcta ggcatcgaaa tgttcgtatt agatgatggc tggtttggaa
    11941 aaagagatgc ggacaattct tcattaggag actggttcga gtatgaaggc aagttgacaa
    12001 atggattacg tgaaatagca gattatgctc atagcaaagg attgaaattt ggtttatggt
    12061 ttgaacctga aatgatctcg gttgattctg aactgtatcg aacacatcct gattttctta
    12121 tgcaggagcc aggtagaatg ccttctgctt ccagatcaca acatgtactg gattttacaa
    12181 ggttagatgt tcggcagacg attgagaaac aaatgagaaa gattttagat acgattcctc
    12241 ttgactacat caaatgggat atgaaccgtt cgctttcaga tgtctattcg attacccttg
    12301 acccacagag acaaggagaa gtagcgcatc gttatatgct cgggctttat gaactacttg
    12361 aacatttgac aactgattat ccggaaatct tatgggaagg ctgttctggt ggaggtggac
    12421 ggtttgatgc cgggtttatt tattatatgc cgcagtcatg gacaagtgat aacacggatg
    12481 cagtggagcg tatgaaaatc caatatggaa caagccttgc ttatccgatt tcgtccatca
    12541 cagctcatgt atcagctgta ccaaatcatc agaccgacag atctacttcg ttgaaaacaa
    12601 gaggagaaac agctatgagc gctgtttttg gttatgaact ggatttgaca aagctgtctc
    12661 ctgaagaa
//
LOCUS       DACEPW010000071        12201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEPW010000071 DACEPW010000000
VERSION     DACEPW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.072"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1270
                     /locus_tag="IW819_001536"
     CDS             <1..1270
                     /locus_tag="IW819_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1244647.1"
                     /translation="GMKLKKAFMSALTLGIAFSGMSMVIGYMSNAVSPASEALAKNTG
                     ISLPALDLGWTGAASITWSWSYAFIFFAVTIGVNFVLLLFNWTKTLNVDMWNVWGKAL
                     TAYLVYYVSGSLAAGFLTAMVQVILELKLGDMFQKHIQDLTGIPLVTVTHFMTSAAVL
                     LLPFNMIMDKIPALNKRADTNALKKKIGIFSENSVMGFIIGLGLGFAAAYGVSGSLNL
                     AIQTATALSLFPMISKMFMQSLSPLADAMSEFMKKRFKDREVYIGLDWPILAGRSEIW
                     VTAILLVPVFIGYAIILPGNQVLPLAGIINYSIAVGGLLLTGGNLARMLILGIITMPI
                     YLYGATYLAPILSDLAEKSGAVEGIKKGQLITWSSIEGPEFRVLFAEAFNRNILAIVG
                     CVIFLGLFVLLYKYMIKAKVPSERYAELQK"
     gene            1292..1552
                     /locus_tag="IW819_001537"
     CDS             1292..1552
                     /locus_tag="IW819_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244648.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            1566..2318
                     /locus_tag="IW819_001538"
     CDS             1566..2318
                     /locus_tag="IW819_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1244649.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2412..3017)
                     /locus_tag="IW819_001539"
     CDS             complement(2412..3017)
                     /locus_tag="IW819_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1244650.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3070..4044)
                     /locus_tag="IW819_001540"
     CDS             complement(3070..4044)
                     /locus_tag="IW819_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1244651.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4422..5201)
                     /locus_tag="IW819_001541"
     CDS             complement(4422..5201)
                     /locus_tag="IW819_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1244652.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5268..6539)
                     /gene="celB"
                     /locus_tag="IW819_001542"
     CDS             complement(5268..6539)
                     /gene="celB"
                     /locus_tag="IW819_001542"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1244653.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            6692..8089
                     /locus_tag="IW819_001543"
     CDS             6692..8089
                     /locus_tag="IW819_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1244654.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8170..8610
                     /locus_tag="IW819_001544"
     CDS             8170..8610
                     /locus_tag="IW819_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1244655.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            8758..9084
                     /locus_tag="IW819_001545"
     CDS             8758..9084
                     /locus_tag="IW819_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1244656.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9085..9834
                     /locus_tag="IW819_001546"
     CDS             9085..9834
                     /locus_tag="IW819_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244657.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(9914..10369)
                     /locus_tag="IW819_001547"
     CDS             complement(9914..10369)
                     /locus_tag="IW819_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1244658.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(10796..11071)
                     /locus_tag="IW819_001548"
     CDS             complement(10796..11071)
                     /locus_tag="IW819_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244659.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11365..12180
                     /gene="thiD"
                     /locus_tag="IW819_001549"
     CDS             11365..12180
                     /gene="thiD"
                     /locus_tag="IW819_001549"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1244660.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         3900 a         2115 c         2484 g         3702 t
ORIGIN      
        1 agggatgaaa ttaaaaaaag cattcatgtc tgctttgact ttagggattg cttttagcgg
       61 aatgtctatg gtcattggct atatgtctaa cgcagtaagt ccagcatcag aagcattggc
      121 taaaaatacg ggaatcagtt tgcctgcact tgatcttggg tggacaggtg cagcaagcat
      181 cacatggtcg tggagttatg cttttatatt ttttgcagta acgattgggg tcaattttgt
      241 actgttactt tttaattgga cgaagacgtt aaatgttgat atgtggaatg tttgggggaa
      301 agctttaact gcttatttag tttattatgt ttccggtagt ttggctgcag gctttcttac
      361 tgcaatggtc caagttatat tggaattaaa gcttggtgat atgtttcaaa aacatataca
      421 agatctcaca gggattccat tagtaacagt gactcatttt atgacatctg ccgctgtctt
      481 gttgctgcct ttcaatatga tcatggataa aattcctgct ttaaataaac gagcggatac
      541 gaatgcattg aagaagaaaa ttgggatctt ctctgaaaac tctgtaatgg gattcattat
      601 tggattgggg cttggctttg ctgctgcgta tggcgtttca ggttctctta atctagcgat
      661 ccaaacagct acagcattaa gtttgtttcc tatgatctct aaaatgttca tgcaatcttt
      721 gtctcctcta gcagacgcta tgtcagaatt tatgaaaaag cggtttaagg atcgtgaagt
      781 atatatcgga ttggactggc cgatattagc tggtcgttca gaaatttggg ttactgctat
      841 tttacttgtt cctgtgttta ttggttatgc cattattttg ccaggaaacc aagtattgcc
      901 attagcaggg attatcaact attcgattgc ggtcggcgga ttacttttaa caggaggaaa
      961 cttggctcgt atgctgatct tgggaatcat cactatgcct atttatttgt acggggccac
     1021 ttaccttgcg ccaatactca gtgatttagc agaaaaaagc ggagcagtcg agggaatcaa
     1081 gaaaggacag ttgatcacat ggtcatccat agaagggccg gagttcaggg ttttatttgc
     1141 ggaggcattt aataggaata ttctcgctat tgtaggatgc gtgatttttc taggactgtt
     1201 tgttctactt tataaatata tgataaaagc gaaagtaccg agtgaacgtt atgctgaact
     1261 acaaaaatag cattgaaatg gtgggaaagt aatgaaaaga gaagagacag ataaaataaa
     1321 atggacagtc gctctttgtg gtacacttct gctatttcta tatggactct ttactcagaa
     1381 tatcatcatc aatctattgg tcattttttt cgcactagtg atctataaat atggaaatca
     1441 cgttttattc agagagtacg atgaaaaaag aaaacggaaa atagaagaat caatgaaaat
     1501 aaaagaagct accaaagaaa tattgagaga aaaaagtttt ataaaaagat aatggagggg
     1561 tcattatgtc agatggacgt atgttatttg agagagaacg acaggacatg gcacgtatcg
     1621 taagagtgat tttcgaaagg aaaaacacaa atgtcgcagg agggaatttt tcctttaaaa
     1681 ccactgatga tcaagggaaa gactacatca tcatgacgcc gaccatgatg tcagaagcct
     1741 atctaggaga gatttctcct gcacaggttc tagtagtaga accaagaacg cgtagaatcg
     1801 ttgacggagt cggtcattta actcgtgaaa tcaatatgca tgaagcgatg tatgagacta
     1861 atccagctat caaagctgtc ctacatgctc atgcgcctaa tgcaatgttc tgggcgacta
     1921 ctgggttgcc tatgcctaat ttgacagaag ccacagctaa agtaaaagag attcctgtca
     1981 tggactttga gcctaattgc tctgaagaac ttgctgaatt gacaagcgac tatgtaaaaa
     2041 aacagcatct cgaattaccg catatgttgc tgctcaactc tcatggcgta ttgatcaaca
     2101 caggtggaga atcaggaatc gaagctatcc ataaagcact gcaaatcctt gatacagttg
     2161 aatggaatgc agaaattgcc tacaagcaga cgatttttca agcattaggg attactgacg
     2221 gtttttattc aaaaggtgag aaaatcgggg gattggaaga gttgaaacat aaaacggcta
     2281 tttataataa atctgttatt gcagctggtg gagactaacc tatctgacac aaaaaactac
     2341 gactttcaca aaatctgatc cagatcgata tctgcttcag gttgtattat gtaagtcgta
     2401 gttcttttct tttatttagt taagaggtgg tctgtccatt ccctcgcttg cttgatttcg
     2461 tccatcgtca actgatgacc tgttacagtt agctgaatag tcacatttgc ttggcgtgtt
     2521 tcgaattgat cggccaattc tttaaaggaa gcaggggaaa cgattggatc gttatcacca
     2581 taagaaatcc agacggtttt atcctctaat gtggaacggc tttgatgaac acctagagac
     2641 atcggatgga aaaacaaccc tttatttagc tcggtctcac gctccaataa taaatgagca
     2701 gcgatgtttg cgccatttga atagccgaca agcagccagt cacttaacgg tatcagttct
     2761 ttttcgctga tttctttgat tttttcatgg agtttatctg tttctttttc caaactgtcc
     2821 aggtcgaatt gattcaatcc attacgcttg aaaaaacgat tcatcccatc ttcttggacc
     2881 gtgccacgaa aagaaagcaa tgtactgttt ggtgcaagaa agtgagcgat atccagtaaa
     2941 gaatgttcat cgccacctgt gccatgaagt aagatcaaat gcttgccact tgttcctttt
     3001 tcaaaaatgt aggtcatttt gttccctctt ttctgtttct atagatgcaa atgaagtagc
     3061 atgaagagat tattcacgtt ttttatttgc atcacttgta tcgaaaggac ggacataagc
     3121 ttcaatggct tcacgacgtt cttctaagaa tggaggaaga gatagttttt ctcctgctga
     3181 ttctgctggt tcatcttcaa ggaatccagg accatcagtt gctaattcaa acagtacgtg
     3241 atgagcggct aggaagtact cagattggaa atagaaacga tcgacgaaac cagaatttgg
     3301 caattcaatc ttattgatta cttgaatcca gtattccaat gcttctttgt ctgctacacg
     3361 caatgctaag tgatggatat ttccaaatcc ttcgattgct tttggcaagt ctgtccgatg
     3421 ttcaataatg atgcttgcac catttccccc ttcaccgatt tcaaataagt agaaatctcc
     3481 tgaatggtct gtttctttga atcctaagat tttttcaaga atcaagcgga tatgctctaa
     3541 ttgactgaca cggacaaaga caggtccaag tcctgtgatc gcaaactctg tcggtacatt
     3601 gctgtttttc caaggaatgc cagctgctac accttgattg ttttcgtcag agatcaattg
     3661 ataatactga ttatcgaaat cttcaaattc gatatatttt ttgccgaaac gttcattgat
     3721 tgctgaatga gtaacgtcat atttttcaaa acgttccaac caaaagtcta atgctgcgtc
     3781 acttggcaca cggaaagaag cacgagagat cgtatctgtt cctttttttc cttttggctg
     3841 atttggaaaa tcaaagaaag tcatatctgt tcctggcgca ccagtatcgt ctgtaaagta
     3901 aagatggtaa gtttcgatgt catcttggtt aactgttttt ttgattaagc gcattcctaa
     3961 aatatctgta aaaaagcggt agatttttgt agcgctagat gtcattgctg tgacgtggtg
     4021 aattcctctg atttgtgtat ccatgataaa tttcctcctg ttaacttaca aaaagtaagt
     4081 agttgattta gacatagtat agagggtgca tctctatttt gcaattaatt tgtcttctaa
     4141 agtatcagga aggaatcaag aatggaaaag aggagggacg gatcaataaa ataaaaagac
     4201 ttacgactag ctgtatcgat ttcaaaaaaa ctagctttaa aaagctaact ttagaatcat
     4261 tacgggtggt cataagtctt tttatgctga gttatttttt catttttgag ctaatgctta
     4321 ggatagaata gaatgttgat ttcacgatct cttcttgtta cttgaaactg cttaccactg
     4381 tcacgatctc ttggtaaacg gctttttcac aacctctttg gttaagttga acgcacatcc
     4441 agcacagaat agttccgaac atcaaatcgg tttcggctgg aagaatattc aatcggctgt
     4501 ccatctttga gatagacgac ttgctgaact tccagtacag gatcgttttc tgcacagttc
     4561 aaatattgct gatcatattc atcactttta tctgcagaaa tagcacgata agcccctgca
     4621 aaatggacat tcaattcttg atgaagatga tcatagatag aggctaacaa atgttctttt
     4681 tttaaacccg gcacaagatg gactggcata aaggtatgct ctaaaacata tggctcgcca
     4741 tctaagatcc ttaaccggtg aatattgtag acaggttgct cggcatcgat catcaatcgt
     4801 tcttggatat attgattagg gaattcaaca tcaaaatgaa tgatttgact cttcaaagaa
     4861 cgatgtctgc gtttcatttg gagactcaaa ccttcatact cgttagcggg cgatgtgtct
     4921 ttttgcataa atggatgatt caagattttc gttcctgatc ctcgctgcga atagacaagt
     4981 ccttccattg ctaagatatt gatggctttt ttgattgtca tccggctcgc accaaattct
     5041 tttaccaagt cgacttgatt aggcaaaaaa ctatcgagag gatactcttt tgaacggata
     5101 cgttctctta aaatatttgc gatttcttcg tattttggca cgttattcac ctcttctaga
     5161 gtataacgaa agattatgct tccgacaact cgaagtagca tctggaacat tgtttattcg
     5221 aaaaaagagg tcatgacaag tttttgtcac aaccccttaa tgatctatta agcaatatct
     5281 aaatctggtt gtttatctag tactttcagg aatgggtacc agataaaggc aacgatgaca
     5341 aagtcgatga cttgcagcat accaccaagc catgaatttg ttgctaagac accgcttgca
     5401 atgattggca cagtccatgg aacagagacg ccagttggtg cagggacaag tccagcagcc
     5461 attaccaaat aattgaacgt cgttacgatt agaggtgcca caacccaagg aataaggatt
     5521 gttgcattca atacgatcgg tagaccgaag atcgctggtt catttacatt gaaaatacca
     5581 ggtgctaaag ctaatcgtcc aacatcacga tactgtttct ttttaagaac aaaggccatc
     5641 aataaaacga cagctaaagt cattccagag ccaccaatac caacagtgaa ggtttccatg
     5701 aatggttttg tgatgatgtg tggtaattct tttccatgtt gataagcttc taagttatca
     5761 agcatcagtg tattccaaat cgggtccatt acagagttga cgatgatttg tccgtgaagt
     5821 ccgaagaacc ataagaattg gacaaagaac aaagcaatca atgttgcagg taaactactt
     5881 cccaaaccag ttaatggttt ttggatcact tcataaacga cgtcatgtaa gttagcattg
     5941 aaaataccag tcatcacagc attgattact aagaaaatag ttagtgtcac tacagcaggg
     6001 atcaaagcgg caaatgattt tgttactgca ggcggaactc catcaggcat tttgatctgc
     6061 cagccgcgtt tcgtgattct gcagtagatt tctccagcca agaaagcagc gatcattcca
     6121 atgaacattc cttttgcgcc aagacgatca agtgaaagca caccagtcac atcttcaccg
     6181 ccagcggttt tcattgcaaa aggcgtaaga atcaagaagc tggcaaaaga cacggcacca
     6241 ccgaaaatac cttcaacgtc ataagatttt gataaataat agccgatacc aaaagtaaca
     6301 aaaaccgaca tgatgctcat tgttgcattt tgcccattac cgaataaatt cgataaagtt
     6361 ccctttgttg cgtcactgaa aaaaggcaag ttattgataa cgacaacgat cgaaccgaac
     6421 atcgttaaag ggaaggaaag catgaaagca tcccgcagca ctgtaaggta acgattttgc
     6481 cctaataaat tagctaaggg catgattttc tcagaaagct tgtccataaa accgttcatt
     6541 tttattcacc tcaaatttat ttgcaatcga ttacatacaa agtataacgc caaaaatttt
     6601 aaatgtctag tcattttcat aaaaagtatt gatttatttt ttagggttcg ttatgattac
     6661 ggtatcaaga aaaaagagag gaagtaaaaa aatgaaaacc tacacattcc cagaaaattt
     6721 ttggtggggt tctgctgcta gcggaccgca aacagaagga agagtaagtg atgatggtaa
     6781 aggagaaaat atttgggatc attggtatga attagagcct gaaaaattct tcgatcaagt
     6841 aggacctgaa aaaacttcgc aagtttacac aaaatacaaa gaagatgttc agttaatgaa
     6901 agaaactggc cataacacat ttcgtacatc tatccaatgg agtcatttga ttccagaagg
     6961 gacaggtgaa atcaatcaaa aagctgtttc tttttatcga gattatttta ctgaattgct
     7021 ggataatggt attgaacctt ttgtgaacct ttaccatttc gatatgccac taccactgca
     7081 agaaaaaggc ggatggttat caagagaaac ggtcgacgct tatgtttctt atgcagcagc
     7141 gtgctttgaa ctgtttggag atctggtcaa aaaatggttt actcataatg aaccaatcgt
     7201 acctgtagaa ggaggctact tgtatcaatt ccactatcca aatcatatca atatgaaaga
     7261 agcggtccaa gtaggctatc atgagaatct agcaagcgca aaggtaatca aagcctacca
     7321 tgaaggcgga catgatggtg agatcggaat catcttgaat ctgacaccaa gctatccgag
     7381 agatgaaacg aacccagaag atgtaaaagc ggcgaagatc gctgacgcat tcttcaaccg
     7441 atcattcctt gaccctgcag tgaaaggaga gtttccacaa gagttgatcg aaattgtcaa
     7501 agaattggct ataatgccag agatccatga aggagaccta gaactcatcc gtgaaaacac
     7561 gattgacctg ctaggaatca attactatca gcctcgtcga atcaaagcaa aagaaactcc
     7621 aatcaatcac gaacatagcc ccatgccaga tgattatttt gataattacg acatgcccaa
     7681 taaaaaaatg aatccatatc gtggatggga aatttacgaa aaaggaatct atgatatttt
     7741 aacgaataca agagaaaact atggtaatat caaatgctat atttctgaaa atgggatggg
     7801 ggtcgaagga gaagaacgat ttgttaacag cgaaggaatt attgaagatg attaccggat
     7861 cgagtttgtt acggatcatt tgaaatacgt ccatcaagca atccaagaag gaaccaattg
     7921 tgtgggttac cacatgtgga catgtatgga caactggtct tggacaaatg cttataagaa
     7981 tcgctatggc tttatcgctg ttgatttggc acaagacggt aaacggacga tcaaaaaatc
     8041 aggtcgctgg tttaaaacgg tctctgaaaa aaacggattt gatgcctgat attttttgat
     8101 aaaacgttca caaaatgttt gttctaacac tgtaatcgtg ctacactaac agtgaaggag
     8161 gcgtttacaa tggtagatac ttatcgtaat attttagtag caattgatgg ttctgaaaag
     8221 gcagaaaaag catttcttga ggcaatcacg attgccaaac gaaatcaggc aacgttgcac
     8281 atcctgtatg tcaatgaagt aacaggcaat tactttggtg attttgcatt tgttacaaca
     8341 agccttcagg aagaattgga tgaggttgcg gaaaaccaga tgaaagaaca tcgaaacttg
     8401 gcaattgaaa aaggcctgac agatatcgag acatatgttc tttacggtta tccaaagaca
     8461 ctgattgcta atttccatga atcgaaagaa ccaatcgatc tgattgtcat gggggcgaca
     8521 ggtctgaatg cggtagaacg tgcactagta ggttccacta cttcctatgt agtcaaccac
     8581 gcatcatgta acgtcatggt tgtaaaataa ataaattaag ggaacagaaa gcaaaaaagc
     8641 ataacgcttc ttttgctttc tgttcctttt atttttttgt aaaaaattac tttacctgat
     8701 gaagtaatca tgctattcta attacaacga cagatggagt aatatggagg aaagaaaatg
     8761 atttcaagtg atggaatacg cggatacaat gatgcgatta ttttatcgat ccttaacaaa
     8821 aacgattctt atggatatga aatttctaaa acgattcgtg agaggacaaa tgattcctat
     8881 gtgatcaaag agacaacact ttactccgtt tttgcccgtt tagaaaagaa tcaactgatt
     8941 gtttcgtatc ctggagaagt gactcatgga aaaaagcgga catattataa aatcactgaa
     9001 atagggcgta atcttttaga agaaaaaaag caagagtggc tgacgacgaa agacgtagtc
     9061 gatcaatttt tggagggaaa ttaaatggat acaatccgta attatttaga cagtcttttt
     9121 atcggagtac ctcaaagtac cgagatagat aagctgaaaa ctgatttgtt ggcaaatatg
     9181 gaagaccatt atcatgaatt gatgggagaa ggaaaaaatg aacaggaggc catcggtaca
     9241 gtaatttcga catttggttc aatcgatgaa ttattagaag aactagatgt cgaaaaaaaa
     9301 catcaagctg acgagacgga aacaaacaca gcttctattt atttaagcga agcagaaaac
     9361 tactggaaag aatacagagc ggcaagtctc caagtggcat caggtgttct attcattagc
     9421 ttatcttttg caagttttct tttcttttgt tcagcaggat atgtgttcat gggtatttct
     9481 tgtctgattt tcggtatagc attagctgtc ggttttttta ttgcttctgg tatgaaaatc
     9541 acacgtttaa atcacttttt gcaccatcgg aaaatacctg aaaaagtatt agcagaagca
     9601 aaagaaaaag aagaagagta tcagcgaagt tttggcttct cactgattgc aggaattgga
     9661 ttatgtattt tttcactttt cccacttttg gcttctttaa tgtggtatat ggatggtagt
     9721 atcggtgcct ctatattttt tgttacggta ggaacgggtg tcttcttgat catttatggc
     9781 agtctagtcc gtcattctta tcgtcaattc actcaaagcg cttattactg gtgaatgtat
     9841 tcagatttgt aaaaatattc tatttatccg gcgtagaaaa aaagactgtg acacgctttt
     9901 gtcacagtct tttttatata taataattta aaaattcatc atctaaatcc gtagaatttt
     9961 ctagattgct tacccatgta aaatcgttat agacagattt cagttgactt ctgcgccatt
    10021 gtacagggat aaaaagacgc atgctatctt cattgaatcc gactaataat ttctttccgt
    10081 cagtaatgat gggacgttgt aacaaagtaa aatgcgagac gatcagatcg acaagctctg
    10141 aggtcgtaca ttggtcgatt tggtctttca aatttttata atcaatcgag cgtttagcta
    10201 atagatcgtc aaatccgttt tctgttaatg acaataaata aaatatctct ttccttgtta
    10261 aaggctgttt aggtgtgaaa attcgttctc tgtaaggcat ttgatggtct ttcagccagt
    10321 ttgccatcag atggtaacta tgtgtgcgag aagaatagat cgtaagcata aaaaaactcc
    10381 ttaaatagtc cattaatttg ttagatctgt tgagataaat aataggtaag gagctgtgca
    10441 taagcattga tcaaaacaac ccaaacatca ttagaaataa ctttctaaag tcttaaactt
    10501 gcgacgagaa acaactagac taatagaaaa aatgtcttag ccatttggct tcctgtttgt
    10561 ttttttgctt atgctgcagc tcctcgggaa agggttctaa caatttgaag aaggttgatg
    10621 attgatctag tttaaattgt gtgaaatttg aaatctggtg tttttactag atgaatcacc
    10681 taacaactat gattataact tatagaactt ctgttttgca ttcggttttg ttatagttgt
    10741 tataaagttt tattttatta cagaacattt ttaattaata aagaacagtt agaagttaaa
    10801 attgctgatt cacatagagt ttatgacttc ttggaaattc tttttcaatc agttttttca
    10861 tctgtttatg aaattctttt ttttcagtaa acataaaggt ttgggtatcg taaccttctt
    10921 catgtaggaa atagattatt cctttttctg tgttttgcgt ataagctttt atactcctgt
    10981 cttttttgaa agaacagatc gtatagccat ctatttgctc tggtagtcgt ttgattttct
    11041 tcaaaatgac ttttttatcc tcaagtttca tttttttcac ctgactttat ttttttcata
    11101 tggaaatcat agcgcctttg gttatatgaa aacaagaagg atactgaaat atatataaat
    11161 caaatagtgg ttatttatta ttagaatagc gaatacaaat aaacgaccac tattttctga
    11221 tttattgcaa atgttttttc aaataaattt gattaccaag aaaaggtagt ggtatattta
    11281 ctgagggata ctttgaaaaa catatgatag aaacagtaac agtattatga aaaatctttt
    11341 tataaaaatg gaggaatcga tttgatggaa gatcagtatg tacaagcaat gacgatcgca
    11401 ggatcagata gcgatggaag cgcagggatg caagcagata tgcatacatt ctttacaaga
    11461 aatgtttacg gtgtttctgt catgaccgca tgtgttgcag gtaattctta cggaatcgga
    11521 gcaagtgtga cattgccaac agattttatc gacaaagaat ttgaactgat tgcgaaggac
    11581 tttcaaatac gtgcagctaa aacaggtatg ctagcagatt caaagttgat tgagacagtt
    11641 gtaaaaaatt accaaaaata tgattttgga ccattagtcg tggatcccgt aatcgtaacg
    11701 aaacatggaa atcttttact agaagaatct gctttgcaaa gcttgaaaga aaagcttgtc
    11761 cctttggccg aagtactgac acctaatttt tatgaagcag aaaagctgac tggacggaca
    11821 ttagcagctg aagaagattt tgtgcaagca gcaaaagaac tgcaagatat gggtgcaaaa
    11881 aacgttatga tcaaaggaca tcatgctaaa aatgcgcagc aagaaacagt caatgactac
    11941 gtattgttcg aatcaggaaa atcattttgg ttaaatgcac cattttatcc taccgaccgg
    12001 atcaacggaa ctggcgattc gctatctgca tgtattgcag ctgagttagc gaaaggaaaa
    12061 acagttgaac aagcgatccg attggcaaaa gactatgtaa accgtgcaat tcgtgaagaa
    12121 ataaatgtag ggcacaaata tggccccatc aatcattggg cagctggtga attagagtaa
    12181 aagaaagaag ttgtgaaaaa g
//
LOCUS       DACEPW010000072        12074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEPW010000072 DACEPW010000000
VERSION     DACEPW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.073"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(133..1440)
                     /locus_tag="IW819_001550"
     CDS             complement(133..1440)
                     /locus_tag="IW819_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1244661.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1443..2024)
                     /locus_tag="IW819_001551"
     CDS             complement(1443..2024)
                     /locus_tag="IW819_001551"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1244662.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2140..2237)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2596..3888)
                     /locus_tag="IW819_001552"
     CDS             complement(2596..3888)
                     /locus_tag="IW819_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1244663.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(3885..4508)
                     /locus_tag="IW819_001553"
     CDS             complement(3885..4508)
                     /locus_tag="IW819_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244664.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4511..5347)
                     /locus_tag="IW819_001554"
     CDS             complement(4511..5347)
                     /locus_tag="IW819_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1244665.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5364..6251)
                     /locus_tag="IW819_001555"
     CDS             complement(5364..6251)
                     /locus_tag="IW819_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1244666.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6267..7550)
                     /locus_tag="IW819_001556"
     CDS             complement(6267..7550)
                     /locus_tag="IW819_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1244667.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8233..8916)
                     /locus_tag="IW819_001557"
     CDS             complement(8233..8916)
                     /locus_tag="IW819_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1244668.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(8934..9551)
                     /locus_tag="IW819_001558"
     CDS             complement(8934..9551)
                     /locus_tag="IW819_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1244669.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9561..10385)
                     /locus_tag="IW819_001559"
     CDS             complement(9561..10385)
                     /locus_tag="IW819_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1244670.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10378..10938)
                     /locus_tag="IW819_001560"
     CDS             complement(10378..10938)
                     /locus_tag="IW819_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244671.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11112..12068
                     /locus_tag="IW819_001561"
     CDS             11112..12068
                     /locus_tag="IW819_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1244672.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         3630 a         2653 c         2047 g         3744 t
ORIGIN      
        1 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
       61 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
      121 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
      181 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
      241 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
      301 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
      361 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
      421 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
      481 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
      541 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
      601 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
      661 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
      721 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
      781 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
      841 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
      901 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
      961 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     1021 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     1081 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     1141 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     1201 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     1261 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     1321 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     1381 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     1441 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     1501 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     1561 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     1621 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     1681 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     1741 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     1801 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     1861 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     1921 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     1981 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     2041 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     2101 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     2161 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     2221 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     2281 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     2341 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     2401 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     2461 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     2521 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     2581 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     2641 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     2701 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     2761 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     2821 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     2881 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     2941 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     3001 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     3061 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     3121 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     3181 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     3241 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     3301 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     3361 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     3421 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     3481 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     3541 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     3601 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     3661 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     3721 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     3781 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     3841 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     3901 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     3961 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     4021 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     4081 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     4141 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     4201 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     4261 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     4321 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     4381 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     4441 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     4501 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     4561 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     4621 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     4681 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     4741 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     4801 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     4861 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     4921 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     4981 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     5041 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     5101 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     5161 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     5221 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     5281 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     5341 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     5401 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     5461 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     5521 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     5581 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     5641 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     5701 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     5761 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     5821 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     5881 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     5941 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     6001 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     6061 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     6121 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     6181 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     6241 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     6301 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     6361 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     6421 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     6481 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     6541 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     6601 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     6661 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     6721 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
     6781 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
     6841 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
     6901 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
     6961 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
     7021 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
     7081 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
     7141 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
     7201 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
     7261 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
     7321 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
     7381 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
     7441 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
     7501 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
     7561 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
     7621 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
     7681 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
     7741 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
     7801 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
     7861 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
     7921 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
     7981 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
     8041 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
     8101 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
     8161 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
     8221 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
     8281 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
     8341 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
     8401 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
     8461 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
     8521 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
     8581 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
     8641 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
     8701 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
     8761 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
     8821 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
     8881 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
     8941 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
     9001 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
     9061 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
     9121 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
     9181 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
     9241 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
     9301 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
     9361 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
     9421 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
     9481 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
     9541 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
     9601 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
     9661 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
     9721 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
     9781 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
     9841 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
     9901 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
     9961 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    10021 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    10081 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    10141 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    10201 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    10261 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    10321 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    10381 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    10441 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    10501 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    10561 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    10621 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    10681 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    10741 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    10801 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    10861 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    10921 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    10981 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    11041 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    11101 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    11161 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    11221 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    11281 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    11341 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    11401 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    11461 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    11521 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    11581 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    11641 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    11701 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    11761 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    11821 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    11881 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    11941 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    12001 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    12061 atatgtaatt ttaa
//
LOCUS       DACEPW010000073        11924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEPW010000073 DACEPW010000000
VERSION     DACEPW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.074"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            567..1538
                     /locus_tag="IW819_001562"
     CDS             567..1538
                     /locus_tag="IW819_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1244673.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1616..1852)
                     /locus_tag="IW819_001563"
     CDS             complement(1616..1852)
                     /locus_tag="IW819_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1244674.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1821..1952)
                     /locus_tag="IW819_001564"
     CDS             complement(1821..1952)
                     /locus_tag="IW819_001564"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1244675.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2028..2411)
                     /locus_tag="IW819_001565"
     CDS             complement(2028..2411)
                     /locus_tag="IW819_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1244676.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2444..3229)
                     /locus_tag="IW819_001566"
     CDS             complement(2444..3229)
                     /locus_tag="IW819_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1244677.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3407..4507
                     /locus_tag="IW819_001567"
     CDS             3407..4507
                     /locus_tag="IW819_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1244678.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4497..5321
                     /locus_tag="IW819_001568"
     CDS             4497..5321
                     /locus_tag="IW819_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1244679.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5327..6133
                     /locus_tag="IW819_001569"
     CDS             5327..6133
                     /locus_tag="IW819_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1244680.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6130..6960
                     /locus_tag="IW819_001570"
     CDS             6130..6960
                     /locus_tag="IW819_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1244681.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6967..7998
                     /locus_tag="IW819_001571"
     CDS             6967..7998
                     /locus_tag="IW819_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244682.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8105..9378)
                     /locus_tag="IW819_001572"
                     /pseudo
     CDS             complement(8105..9378)
                     /locus_tag="IW819_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9605..9790
                     /locus_tag="IW819_001573"
     CDS             9605..9790
                     /locus_tag="IW819_001573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244683.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            9914..10630
                     /locus_tag="IW819_001574"
     CDS             9914..10630
                     /locus_tag="IW819_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1244684.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10532..11052)
                     /locus_tag="IW819_001575"
                     /pseudo
     CDS             complement(10532..11052)
                     /locus_tag="IW819_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11340..>11924
                     /locus_tag="IW819_001576"
     CDS             11340..>11924
                     /locus_tag="IW819_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1244685.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKEL"
BASE COUNT         3427 a         2201 c         2722 g         3574 t
ORIGIN      
        1 agtctggata acgtgatttt ttgctgtaat gacaaggttt tagctgtttc agataccatg
       61 tatccaaaaa tagtttattg agaaaatagg agagtatttg atactaacta gtaggaaaaa
      121 atgagataaa aagtaaatgg aatcatttaa ggtaatctga aataaaaaat atacagccct
      181 gtggtacttt acctacaagg ctgtatatat tttttactct cagcgttttt gactccgtgt
      241 ctgactccgt tttattgatt ttgaatgtta ttcaatgacc atttaacagg tcaaatttga
      301 tgtttttgat tccgtatgac gccgtttgat atctagtgat atagtgattt gagtctacat
      361 cgaataaggg aataaaccaa taaaaaatga tttagaatat taaaataaca aaaaagagga
      421 ctacctgtat tagtcctctt ttttgaccag attttgacca gtctatttta catcctagta
      481 tatttatatg agctaatttt atgattaaat ggtaagtttt tccttgtgtg ttttagttta
      541 aactagtgtg taattcaaaa atctatatgg aaggaacagt attaagaata gaacagggtt
      601 ctctacatga tggtgctggt ttacggaccg ttgtttattt aaagggctgc ccattacgct
      661 gtgcctggtg ttcgattccc gaatcacaaa gcaagcagat tgaaaaagga tttggccaga
      721 ccatgaccgc tgaagaggta atggatgaga ttgagaagga tgctgttttt tactttcact
      781 cggatggcgg agtgaccatt agcggaggcg aggcactggt tcaggcggat tttgctaaag
      841 aaattttgca aaaatcgaaa tacattggaa tcaataccgt actggaaacc agcttttgtg
      901 gtgcttataa tgaaatccaa aaggtagctc cctatgtgga tacattgttt gtggatgtaa
      961 aaatgtttac cagcaagctc cacaaacaat ggaccggctt agataaccaa caaattttga
     1021 agaatattcg tcgcttttta attgagtatc ctaactgcga agtgcgaatt cgagttcctg
     1081 tggttccagg tatcaatatg aacctgaccg agctgctgac gattgcttgt tttgtggcag
     1141 atcttgatcg gttcgtaccg ttagaattac taccgtacca ctgctatggt atgcacggtt
     1201 accaagcatt gggactggag tatccgttgg cagacacgcc agcgccttca cctaaagaaa
     1261 tgtttgcatt ggcagatcaa ctggctcgca cggttcctca tttgctggtc acagctttaa
     1321 ccaaaacatt tatttattta ttgaatccaa gctttatctt atggataaat gaaagacact
     1381 cactagctgc agccagctca agaatagagc ttttgctgaa gtcagtgagt gtctttttac
     1441 gatgtttttt cgaagttaat ttgtcgaatg aacactggct caatcggtac atcttcactg
     1501 tttgtcggct gagctgcgat cgctgctacg atttctaacc cttggtatac ctgtccaaaa
     1561 acagtataac gaccatccag ctccggtagt cctccaagct ccaagtatgc ttgctttaca
     1621 ttagcaggtc agcctgcgcc tttgtgtttt gcacaataaa aaactggttg ccgttggtgt
     1681 gggggccgtg gtcccctaag gccaaggcgc cattaaaatg ataaagtccg cgatgcactt
     1741 catcagcgaa aaagccctca tagctggaaa cagcttcatc accagaacca tccaaagcgc
     1801 caccttgaat aacaaattcc ttaatgacac gagccgaaag gtgtgttgtc ataaacgcct
     1861 tgctcagcta gggagcgcca gttggctaca gccaggggag cttggtcagg aaataagcgg
     1921 agacgaattt caccagcatc ggtgaccagc acacaaactt cttcctgctg cggtttttct
     1981 aattgaaaga gaggaaggtt tgcaactgaa acgccttgat cctgccatca aatatcgaaa
     2041 atccgccact cgaccttcgc ctgatcacca aggcgagcca ttcccaataa aacggtatag
     2101 gcggtttcat ccgcttgaaa ggtcaattgc agctgtcctc gtaaatcgtt gtagtcatct
     2161 ggggcggcta cttgataaaa aagatgaaac tgcgcatgca aatattccaa aggctgatct
     2221 ttaaaggctg caaaataagc ctgaactttt tcccgctgca agctgtcagc caccaaaaaa
     2281 ttttccagtt gttgttttgg aacggctgct aacaaggatc ggcaaaccgc ctcaattttc
     2341 aacgcatccc aatgtcgcgc aattaaataa tggatgggtg cctcttgatt gtagtcccac
     2401 aggttgctca tttttttcac cactttcttc tattatatag gaatcacaaa atcggcagcg
     2461 gcgtcaggcg gcgttctttt ccttgaatga ctagcaactt ttctacctga cagtctgcca
     2521 acagctgact cattttttta aagcccagct gataatcttc ggcaacatga gcatccgagc
     2581 ctaaggtaaa gagctttccg ccgacacttt gatacaaagg aacggcataa cggtaaaggt
     2641 cttcgttgcc gtatttcaag aagctcttgg catttaactc catcgccaat tctcgttcaa
     2701 caaccttttt gaaaatcgtt agcagctgag cctcaaaatg ctccgcgaat tcttccgctg
     2761 tgaaggtgaa gcggcgtaat ccataatcaa agtgagtcaa aatatctcct tctggaaaat
     2821 ccgtcaagac ctgcaacatc tgatcaaaat attccttggt agtttgaatg ggatctttgg
     2881 ttaaaaccac atcgtccata taatcaaagc ggccattttg atgaatactt accagcttta
     2941 aatcataagg atgctgagcg aggtaatcca cgatccgctc ttcttgtcct ggaacaacgc
     3001 caatctcgat acctcgcaga aatttggtgg aataggtttc ttttaaagag tccagtttgt
     3061 ttatataggc tggataatcc ggaatgtcat cttttccggt agatggattg tttaaatcaa
     3121 agtgatccgt agaaacgaaa aactccggct gataggctaa atagttttca aatttttcct
     3181 ctgaatcaaa ggaaaaatac gtgtggagat gctgatcata atactgcata gggagcctcc
     3241 ttttttctac tatcataccg caaaagcttt agaaggttaa cgggaattcg tcgaaattta
     3301 caaaccatat acaaccttta cattgttcat gaaacctttt caagaaactg attctctcat
     3361 tacaaagctt tgttagaatg gaattatcga aaggggagaa gaatgaatga aaatgaatga
     3421 aaaaattcgt aacatgacta tcggtttatt ttgtgcagca acagtaagta tcctgctgac
     3481 aggctgcgtc tcaggtgaag caacgaagca atcggctgcg gcagacgact ctttagatac
     3541 gattatagaa aaagccaagg aagaaggaga gattgccagc ttgggaatgc cggatacttg
     3601 ggcaaattgg gtaggcactt ggaacgacct tgaaaaagaa tacggcttaa gtcacgtgga
     3661 tacggatatg tccagtgcag aagaattggc taagtttgaa tcagaaggaa aaaatggaac
     3721 cgccgatatt ggtgatgtcg gtatcagctt cggtccgttg gctgaaagca aagagttaac
     3781 cttaccttat aagaccaact attgggagga aattccggat tgggcaaagg atgatgatgg
     3841 ggattggttg ttaagctata cgggaaccat ttcctttatc accgataaag agaatgtgaa
     3901 gcaagcaccg actagctgga aggagctgca agagggtgac tatcaagtta gcgtggggga
     3961 tgtgacgatt gccaaccaag cacaatttgc tgtattggct gcggcgattg ccaacggagg
     4021 tgacgaaaag gatattcagc caggtctgaa ttactacaaa aaaatggcag aaaaaggccg
     4081 tttgtccacc gttgatgcca gcgtagcaaa tcttgaaaaa ggtgaaatcg atgtggcagt
     4141 tgtttgggac ttcaatggac taaattatcg tgaccaaatt gactccgagc gttttgaggt
     4201 aactgtgcca acagacggct ccatcattag cgggtataca accttaatca ataaaaatgc
     4261 ccctcatccc aatgcggcaa agctagcacg ggaatatatt ttatctgatg aagggcaaat
     4321 caatttggca aagggctatg cgcgacctat tcgcaaggta gagctgccta aagaagtagc
     4381 tgaaaaatta ctgccggagg atgcttatga agcggcggtg ccagttgaag acaatgaagc
     4441 ttggaacaag acctctgtag agcttccaga gctttggcag gcggaggtac tgacctatgc
     4501 aaagtagact tgacaaactg attgtagtta tactagacgg ctgtcgctac gacacagccg
     4561 tcgcccagct tgggttcatg aatcatttag ttgagcacca gcaagcaagc ttattgaagg
     4621 taaaaagtga gctgcccagc aattcacgtc cgttatacga agtactgatg accggcgttc
     4681 cagcctatga aaatggcatc tatagtaatt accatgctca gcgatccaaa gagtggtcgc
     4741 tgtttgaatt agttaaggaa gctggcggaa aaacaggagc cgccgcttac cactggtaca
     4801 gcgagttgta caatcaagcc ccctataatc gtttggttga tcgcatccag catcaggagg
     4861 agcgactgat tcagcatggc atcttttata gtgaggacac ttaccctgat tcgcatttat
     4921 ttgcggatgc ccattattta gtgaatcagt atcagccaga ctttttggtc gtacattcga
     4981 tgaatattga tgatgtcggt cacaaattta ccgccgattc tcatgaatat gcagcagcgg
     5041 ttaatcaggc ggacagatta ttggctattt accttcctga atggctgcaa aaaggttatc
     5101 aagtcgtggt gactgcggat cacgggatgg atgcgcacgg actgcatggc ggcagcttgg
     5161 ctgcacatcg tgaagttcct ttttatttga tttcaaataa acgccaaaag ctgaaggaac
     5221 aaactgtacc acagctgctg gcggctccat tgttttgctg gttattagga attgcaccgt
     5281 cagagaaaat gcaggacatt caagaattga tgaggggatg aaagagatga aaaaacgctt
     5341 ggttctggtt cctgcctcct ttatcttctg tgcattttta ctgctgccgc tgctttttat
     5401 ggttctgtgc agcttgcaca gtgatttaac taaccagtgg acattggcca attacagtga
     5461 tattttcagc aacaaatatt acacacaggc ctttgttaac agcgtactga ttgctgtcag
     5521 ctccagtgtg attgggttaa tcgggacgtt tattttatgt ttatgcttaa tgaatctatc
     5581 gactaaattg caggagaaaa tcacctttgt ttccaatttg gcagctaatt ttgctgggat
     5641 tccgctggca ttctctttta ttattttgtt ggggaatgta ggtgtgctga aaatgatttt
     5701 gccatttcta gcggacttca atttgtattc ttggtgggga ttgaccatca cgtataccta
     5761 tttccaaatt ccgttagggg tcttgttttt gtatcccagc atgaaggaag tgaaaaagga
     5821 atggctggag tccgcacaat tgctgggcgc ttcatcagcg tatgcttggt tcaaggtggc
     5881 tttgcctttt ttgcgaccat cgattgccag cacctttgtc atcttatttg ctaatggcat
     5941 ggggacctat gaaacagcgt atgccctgac cagcagtaat gtaaatcttt taacgattcg
     6001 aattgcggct ttagtgtcgg gagatgtgtt cgccaagcct aatttaggca gtgcgttggc
     6061 agtcgcttta ggtgtcctgc tggtaacggc aatgttgatt attcaaaaga aaggcaaggt
     6121 ggtgcagacg tgaaaaagtt aataaaaccg attttgttct gctttgtcct gtattacttg
     6181 ctgccgatta ttggaacgat tttgtatgcc agctccacta aatggtcaaa atcgctttta
     6241 cctagtgatt ttaccttgca atggttccaa cagctgctga cggacaggga atttattgct
     6301 gcggtgggtc gctcactttt attagcaggt gtggtcttgg tgacgattct gttgttgatg
     6361 attcccacca ttatttggat tcatttgtat ttccctcgtt tgaatcgttg gctggaaaag
     6421 ctgctcctgc tgccgtatgc tttaccagga gtgattttgg taacggcttt gcttcgaacg
     6481 tatgctgaaa ctggtatccc aatgtttgta gtgttggtag gtgcattgtt tattacggcg
     6541 ttgccgattg tctatcttag tcttaacaac cagatgcgct taattaatct gaaggaatta
     6601 gtggatgcag cagaaacctt gggcgccccg atgtctacga ttattatcca ggtgctgttt
     6661 ccaaacatcc gtatcggtgt gaccctagtt tctctgatga ttttttcttc ggtttttggg
     6721 gagtacatgc tgacgaacct gttgattggc ggtcgatttc agacggtacg catctatatg
     6781 ctgcggcgga tgaacgaaaa cggccatttg gctagcgctg tgatggtttt gtatttgctg
     6841 tttatggtgg gaattgcttt aactaccttt atgctgactt atcgacaaaa gcaacagctg
     6901 tcccttaagc agacgaggga agagaagcag tcgaaaaaga agcccttaaa ggagttgtga
     6961 cggaaaatgt ttttagaaat acatgatctc aatctgtttt ttgatgaaaa acagatttta
     7021 gctattgatg aaattcagat tgaaaagggt gagctggtta ctttactagg tcccagcggg
     7081 tgcggaaagt ctacgttgct gcgctgtatc acgggattgg aacaaccaaa gagcggtgag
     7141 attgtgctgg acaatgagaa tattgccgat aagcctacga aggatcgcaa tatcggcttt
     7201 gtttttcaac agtatgcatt gtttccaact atgactgttt ttgaaaatgt agcctttggt
     7261 ttgaaggtaa agaagctgag tcctgaggtg attcaggaaa aggtttttga gatgctatca
     7321 ttggtggata tgacggagca ggcagataaa aatgtccagt ttctttctgg cggtcaacga
     7381 caacgggtgg cactggctcg ctcattggtc actgaaccga aagtcctgct gctggatgag
     7441 ccattaagtg ctttggatgc ccgaattaga aaacagctgc agcgggattt gcgcgctatc
     7501 cagcaatcgc tagggatgac gatgattttt gtgactcatg accaagaaga ggcgatgcga
     7561 atcagtgatc ggatttttgt gatggaagct gggcgggtgg ctcaggtttc cacctcgaag
     7621 gaattgtatc agaatcctca gagccgattt gttgcggaat ttatcggcaa ctataatcgt
     7681 ttcgaatggt atgagctgaa tcagtatacc cacgagtttt ccgctaaggg caatcgctgc
     7741 atttattatt taagaccgga gctgattcaa tttgaaccgg ttcctcaggg ggttaagatt
     7801 ccggttctat ggaaggaatc atttatttta ggcaacattc aacggtatgt ttttcaaacg
     7861 ctggagggca aagaaatttt agtggatcgg ttgaacgatc gggaagagaa aattagcgat
     7921 gccttataca ttcagctgaa agatattcta gcaatccctg tggaaattga tactcagcat
     7981 tttgcacaac aaagctagtg tatagtcggc aaacatttct ggctctttgt caaataggac
     8041 tgatagagtg atataagaac atctaaggta gaattgattt tctgctttag atgttttttt
     8101 atgtttaata agtgatcaga tgattgatta agaaaattct ggttctcatc ctaacccgta
     8161 agctattcgt ttcaatgctt tgatgtgggt atgcagattt tctattttgc catttgagta
     8221 aggcaactct aaggctaaac gaatgccgga ttcatagttt ataaacgctt tttttatatg
     8281 atgcagttct cttggaaccg acttgggcat tgtttttaca agctgaaaga agatgtccgc
     8341 ttctttgtca taaaaagctt gaagaatttc ttgatagtaa gcataagcta ttcgtagttc
     8401 atcagaagta gaaagtagac gttctacgac ttcactttcg gttaaatagg cccaaccaaa
     8461 attacgccat tttgtccatt tatcataaac aagcgtgtcc tgtcgtttga tcagaagtcg
     8521 agataaagcc ttcacttgtc gatagtgttt ggcttgtatt ggatcgtttc ggtcgaagct
     8581 tttcatgact catagtcaaa tatccttaaa actgcgaatg aggtgcttga cgatgtgaaa
     8641 gcgatccaca acgagtttgg catgaggaaa gcactctttt atcagcaaag cataagaggc
     8701 gttcatatct gttacgagta tttttacgtt taaacgtgca cgtcgttcaa attgcataaa
     8761 ataagaaatg agatctttct tcttacgtga aggaagaata tccagtaatt tcccagtatc
     8821 gccatccata caagaaaaag aatagggacc gacttgattt ttcaatgaac gaaattcatc
     8881 taccaatgaa cagcgaggca accaatttat tttcacgttt gtcatgggga gcagagaagc
     8941 gagtgttcgc gaaacggttg accaagaaac gccgcaatgt ttagaaatcg tcgtcattgc
     9001 ttgtttttca gtcaattcct ccaatatttt ataatgtact tgtttggcga taaaacagtt
     9061 tttttcaaca atgtaggtat tcgccgtaaa ataggtgtga cagccacgac agaggtaccg
     9121 ttgctttgcc aaagcaagat aagtccgttg attcgcacac ttattgagaa ggaccagcga
     9181 ttttttcttt ccgttcttaa cgatcagccc atgttgattc acctgtttac actgaggaca
     9241 gggctgaatc gttttaatga gcgtggcaga aacaacgata ctgttgattg aatgtatttt
     9301 tttcttctcg acatgagtga tttgaatgtt tccatctttg attcctagta atagtttgat
     9361 aagatgaaga tcaggcatac agaacgctcc tttgattgtg ttgtggtgat tcaattctaa
     9421 cggagcttct gtatgttttg tttttttatg ctcaaaagga ctgatagaat gatttactat
     9481 cagtcctaga aattatagcg ccacatttct ttcacctttg acttttcaga cggcagtttt
     9541 tatgctgata ctgtagagag agcaggacac agcttttgct tgttttgggt aagggggaaa
     9601 gaagatgacc aaagcactta tttttgattt tgatgggacg atgattgaca gtgaatggat
     9661 gttttacgag agtattcatc actatttact gaagcctatt tactggagca gttcggctat
     9721 aaaattcctt ttgcagacta tcaacaaggt gttgggacgg cagcagaact gtccttttat
     9781 gaagggataa gtcaagcggt tgggagacca gtggatgctg aacattttga gcagcggatc
     9841 agcagcactg gaaaatcgga gtagtctcaa ttctgaactg cgggagctag actatttttt
     9901 tgaagcacat ccgatgatta aggaaaaaat tgattccttt gtgacgatcg atgatcttca
     9961 ggtcggcaaa ccagatccag aaggttattt gttgtgcttg aaacggctga gggttgaaca
    10021 ggctgttgta gttgaggata gtccagtcgg tgcccaagca gcaccgcata tgacagttga
    10081 tgtacaagcg ttggatgcag attttttcgc ctttagcggg cataaaatgt gcgggccgac
    10141 aggaatcggt gtattatatg gcaaacgtca attgttagaa caaatggagc cagtcgaatt
    10201 cggcggtgaa atgatagatt ttgtcaatct ttacgatagt tcatggaaag agctgccatg
    10261 gaaatttgaa gctggtactc cgaacattgc aggagcaatc gattacttga atgagattgg
    10321 tatggagaat attcatcgtt atgagcaaga actggttgcc tacgttttac ctaaattaca
    10381 agcgatcggc ggtatcacca tttacggacc gcaagatcct gaacatcata ctggcgtggt
    10441 tgcttttaat ttggatggca tccatccccg tgatgtggcg actgctctgg atatggaagg
    10501 tgtcgctgta cgttcaggac accattgtgc acaacggttc atcgacgatt tctgtttgac
    10561 taattggtcg tttaaacagt cgatgataat gccgtttgag cggctccaat gtcgtggaat
    10621 ctttgagtaa gagtttccaa tatcgcttga gccgacggta cttcttctga ttttcagatt
    10681 acgtgattga ttttcgtaca gttctatgag aatcgcctgc ttcagttcct ttgttagttg
    10741 atgatgttca tcaactaagt ccgttttcgc agaaaaggtc tgcccacaat catagcatag
    10801 aaaacgggta cgttttaatt ctaaaatagt caacttttct ttgactttca acatctgagt
    10861 acgtgtcgta taggaaccgt tgcggataat acgaatcgat ccacagtgtt cacaatggga
    10921 aggtgagtca tccaatctcc ctttaataaa ctgcgcttga acgcctttat attctttttc
    10981 aatcagccat tcttcatcaa aaaataaatg tttatctgtt aatccgagta attttctagt
    11041 atgattatcc atagagcgac tcctttggta gggtgatgtt ttggtcgact aaattttacc
    11101 aaaagtgagt ctctttttga gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg
    11161 attttccaac aacacaaaat attatagaag ccttttttct aaaggatttc aaggtgtctg
    11221 tttgcattta ttaaaaaaaa gaaaaagtat tggcaacaga aggcgtcacc aacactagat
    11281 attatagagc cataatatgc tgtataatat ctagtgtata ggcttaagtg gaggtatata
    11341 tgaaaatatt aactgttgaa gatgataata tgataagaga gggaataagt gaatatcttt
    11401 cagaatttgg atatactgtt attcaagcta aagatggaag agaagccttg tcaaaattta
    11461 atagcgatat aaatttggtt atcttagaca ttcagatacc ttttataaat ggtttagaag
    11521 tgttgaaaga aattagaaag aaaagcaatt taccaattct aatcttgact gcatttagtg
    11581 atgaagaata taaaattgat gcatttacta atttagtaga cggatatgta gaaaagccat
    11641 tttcattgcc ggtcttaaaa gctagaatag attctttaat taagaagaat tatggacatt
    11701 tagagaagtt tgaatataag gatctatcgg ttaattttaa tagctataca gctaaaataa
    11761 atggtgaaga aatagatgta aatgcaaaag aactagaagt attaaaatgt ttattggata
    11821 ataatggaca agtgttaaca agaatgcaaa ttattgatta tgtatggaaa gatagcgaag
    11881 aaactcctta tgatcgagta atagatgtat atataaaaga actt
//
LOCUS       DACEPW010000074        11888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEPW010000074 DACEPW010000000
VERSION     DACEPW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.075"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(556..1317)
                     /locus_tag="IW819_001577"
     CDS             complement(556..1317)
                     /locus_tag="IW819_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1244686.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IRERKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            1417..3138
                     /locus_tag="IW819_001578"
     CDS             1417..3138
                     /locus_tag="IW819_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244687.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            3153..4937
                     /locus_tag="IW819_001579"
     CDS             3153..4937
                     /locus_tag="IW819_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244688.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(5318..6871)
                     /locus_tag="IW819_001580"
     CDS             complement(5318..6871)
                     /locus_tag="IW819_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1244689.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7219..9633)
                     /locus_tag="IW819_001581"
     CDS             complement(7219..9633)
                     /locus_tag="IW819_001581"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1244690.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            complement(10060..>11888)
                     /locus_tag="IW819_001582"
     CDS             complement(10060..>11888)
                     /locus_tag="IW819_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1244691.1"
                     /translation="FITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSALSQAPYY
                     NLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRNTSFGNG
                     YYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPASGNAGGG
                     ATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITMDQLIQW
                     NNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKYTVQSGD
                     SVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTGNTSGSS
                     SSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQQLTVSN
                     SGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGITMDQLIE
                     WNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGNTMYTVK
                     AGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVTGSKTYT
                     VKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
BASE COUNT         3462 a         2384 c         2143 g         3899 t
ORIGIN      
        1 ctttgtgatt tttcttgttt gaagaatgtt agaagaaaaa tctctaaata cttataatta
       61 ttttataagc aaaaaaatgc tatcggtagt atcaccgata gctgaggaaa gtcacagtag
      121 ggactatttg attaaaaaat atgtttattc aatccatcta tttatttttg ccttattaac
      181 atattgtaat aatgtaagca acaatgtatt ttaagtgtaa tgcttttatg cacctattta
      241 atttatcatt ctcatctgat acaatctcac tcataacaat atgaattttt cttctcatta
      301 gtctcagttc accatgaatt atacataaca cccaatacaa ttatttttcc atactttttt
      361 aagaggacaa ttacatcgta tatatcgcta agaattgcaa ccattccatt tttaaagaca
      421 tatttcctct aaaaaactaa aattctatct atccacgact aataatcccc cacattaaac
      481 ccgtaatcga accagaaaat ttttctttat aactaaaaca accatcattt tcgagttatc
      541 ttaaacacat atttcctatt ttttcatttc tatccatgca tgataatatt cagttctgat
      601 attgttttca tccgtattcg tcaatagata ctggccgatc acttctcctg ttttccatcc
      661 tttttcatac attctttctc tgatttctaa aatattatta cgttccgcat ggttcatctc
      721 ggatttcaat aagaatcgac gataaacagc cgtatctttt ttctcccaaa tcacgtcttg
      781 agtaaataaa tgtacttcac tagggtcgat aaaaaatcca tacatcgtct gtgaactgga
      841 agctggcata ctcatcatcc caaagcttga atagttaggt agaaattttt tgatttccac
      901 aatatcttca aactccgttg agatcataca gtcaaaaggg atctcttcta caggaaattc
      961 ttcctcgttt gtatcgatca tcaatttcaa ctgttttttt cgctccctta tctctttttg
     1021 tttttgtttc atcaccacca gttctttatg gattcttctt tctgtttcat caagtgtctc
     1081 atataactcc gtcaatgttt ttccatataa attcttcatc tgtttgatcg gtatatctaa
     1141 ttttctataa aaaacgatat cataaagcat aaaaatatca tcgatatcaa acaatcgata
     1201 atcattctct tcttgtctac ttgaacggat taatccttgt tcatcccaat agcggatcgt
     1261 tgattttggc aaatcaaata gttccgatac ttggccaatc gtatacattt ttttcatttt
     1321 ttctcacctc tttttcagtt tattcttttt tctattgacc ttcaagttac ttgaaggtat
     1381 agaatcgaaa agtatcagga aaaaaggagg aataatatgt ctatcttaaa aacattctta
     1441 cagaaaaaca aaggactcgc tctccttacc tttgtgatga tctgtttaca aatcgccggt
     1501 acattaggtg taccaaaatt agtagcaaag ctgatcgaca caggtatagc aagtggcgaa
     1561 gcctcagtta taaaaaaaat tggagttgaa atgctgcttg tagcgtttct aggaacaatc
     1621 gctgcagttg cctctagtta cttttcagca ttggcttccg ctaggtttgg attccaaacg
     1681 cgggagaaat atttccgtaa attccaacag ctgtcaatga aggatatcag tcgttttagt
     1741 tctggatctc ttttgacaag aatgacgaat gatgtcgata atgtccagca aatgattgtt
     1801 ttattttgcc aaatgatctt accagcaccg gttatttgcg ttttcaccat tctcatgatg
     1861 tttcgttatt ctctattact aacatgggtt acattgatct ctgttgtttt ttatgtatgg
     1921 attgtttatc gactgatgaa acgaggggca cctttatctt tgagtatcca gccaaaaatg
     1981 gatcggatca ccatcacatt gagagaattc tttactggga ttaatatgat ccgtgccttt
     2041 gataatcaat catatgaaga aaagcgaacg aataagagct ttgctgacta tgctaatcaa
     2101 atgatcaaag tgaaccgaat atttgcttgg gtcactccaa ttgctttttt gctcatggga
     2161 atcgtttatg cttctatttt gtggtttggc ggaaatcttg tagcagaagg agcattacaa
     2221 atcggtgtcg tgactgctgt agtcgaatac tctatgctga cacttgctta cttgatgatc
     2281 gcagctatgg tattggtcat tatccctaaa tcgtttgcct cattacgacg gatagaagaa
     2341 atactgcaag cagagatcga aattgaggaa gagacgatca aaacagtagc tgaaccaacc
     2401 tttgatcaga caaatgcagc tgcttttgaa catgtcacat ttaaatatac tgaaatctct
     2461 gatccagttc tagaagatat tgatttcaca atacctaaag gaaaaacaac agcgatcgtt
     2521 ggcgggaccg gatctggaaa aagtagtttg gcgaaacttc tcttgagact tagtgaccct
     2581 acgataggaa aagtaaccct tggtggtatt cctcttactc aaatgactca agaacagatc
     2641 cgctcccaca tcagttatgt ccctcagaag gctttcttat tcagcgggac aattttaagc
     2701 aacttgcaga tgggaaatga atatgcctca acagaagagt tgtctaaagc gatcaagatc
     2761 gctcaattag aagaactagt ggacaccctt cctgacggat tggacagctt tgttgctcaa
     2821 ggcggttcta attattcagg gggccagaaa cagcggatat gtattgctcg agcgttgatc
     2881 aaaccagcag agatctatgt attcgatgat agtttctccg ctttagacta taaaacagat
     2941 gcagcattaa gagcagccct tcataaacac atgtcggaga aaactctcgt gattgttgct
     3001 caacgcctca gtacaatcca acaagcagat aacatcattg ttttagatga aggaaaaatc
     3061 gttggacaag gtactcatga agaactactg tctaattgcc aaacttatca ggagtttgca
     3121 aaatcacaag gaatatagaa agaagggata cgatggaaaa gaaaaaaacg ttgaacacgc
     3181 gtgctttgat acgtttttgg aaaatgatca aacctgaaca taaatatttt tacggattgt
     3241 ttctatgcag tttattcggg aacctattag tcgtagccat gcccttgatc atgggaatcg
     3301 ggatcgatga tctcttatca gaaataagaa aaacaggtat tggacagctt tctttcagtg
     3361 atatcaaaca agcattgttg ttccctgtct tattgctgat cggctgttca gtcttaagca
     3421 gcatcacttc atttattcag gaacggacaa tggcagcgtt aagcgaaaag atcacattga
     3481 gggtgcgaaa agaaatcaca aaaaaattca aaacattacc gatgtcgttt tttgacaatc
     3541 atcaagtagg tgacatcatt agtcggacaa ctaccggtct caatcaactt tctcaagtgt
     3601 tattgacagg tatcaatcaa ttttttactt ctgtcgtaac aatagctgct gctggtctga
     3661 tgctgtttta tatcgaaagc aagcttactt tgctcgtctt attgctgatc gttggcagtt
     3721 caattgttac aactaagatt gcgaacaaaa acaaacagat ttctgaacag aatcagtatg
     3781 aactgggtgt tttaaataat cgaatggaag aaatcttagc tggtaatcta gtggtcaaaa
     3841 cattcaatca gcaagctcat gcagaagctg ctatacacga agtaaataaa aagcaataca
     3901 acgcttttaa acgtgctcaa tttatgaact ttgctatcta tccggccatt cgtttcatta
     3961 atcaactcgc cttcatcata agtgcggtat tcggtgctat gcttgttcta tcaggtggtg
     4021 tgacgatcgg cttcttacaa gcttatcttc agtacatcaa tcagatttct gaaccaattt
     4081 ctaccgcttc ttatgtgatc aactcgatcc aatccgcaat ggcagcaatc gataggatat
     4141 ttgagatatt agatgaaaca gatgaaattc cagaccttcc agatgcaaga cttttagaac
     4201 aaccgaaagg aaaaatagct ttcaaaaatg tacagtttgg ttacacatca gataaaatat
     4261 tgatgaatca agtcgatttt tcagtaaagc caaaacaaac ggtagcgatc gttggtccaa
     4321 ctggtgcagg aaaaacgact ttagtcaatc tgctgatgcg tttttatgaa ctgaataaag
     4381 gaaagatcga attcgacggt cttgatatta ccaagctttc acgtggagaa ctacgtcgtt
     4441 cttttgggat ggttttacaa aatacctggc tgtttgaagg aacggtagct gataatatcg
     4501 cctacgggaa aatggatgcc acaagagaac aaattatcga agcggctgaa atcgcacaat
     4561 gtgatcactt tattcgaaca ctgccagacg gatatgatac gatcatttct agcgaaaacg
     4621 gcacgttatc tcaaggacag caacaattat tgacaattgc tcgagtcatt ctagcgaatc
     4681 ctcctgtcgt tattttagat gaagcaactt ctagcgtaga tacacgaaca gaagcattga
     4741 ttcagcaagc aatggaagct gtgacaaaag ataggacaag ttttgtgatt gcacaccgtt
     4801 tgtcaacgat tgaaaatgca gacatgatcc ttgtaatgaa gaatggcgat atcgtcgaaa
     4861 aaggaacaca ttctgaacta ctccaatcac caacgcttta tgccagtctt tataatagtc
     4921 aatttcagat agcgtaacaa acattatcta aaaagctatc gatcatgtat gattagtcca
     4981 tgatcgatag cttttttaaa tgtactagtg gtgccactag aaacacaaag aataatacta
     5041 atgatacttt ttggatagct ccttcagttt cgttcggtaa gtagtgacaa aagacttgtc
     5101 acaatccctt tttacgaata aacggtgttc aagagacaac ctatcggcaa taagacacga
     5161 tcttgtaaaa tacgagaagc tatacttcaa aatcgcttct tgttgctctt gtcaactccg
     5221 aatcacgagt tacactattc aacaaatatg agaagctgtt ttttgaattt gtttcttgtg
     5281 actcggtgct gaacgtcagg ctcacaatct ctcttcttta atctccttgt tctacttgcg
     5341 atagttgctg aacgaattgc catgtttccc cgttatcagt tgaacggaag aggcaggcaa
     5401 acattttccc tgcatcgatt ggtccttttt tcaacaaata aaattttgtc tctaacgtat
     5461 tcgcgcttat ttgagtaact tcatttggtg attgaaaaag atctatcccc cctgtctgat
     5521 agttttccgg aatcgacaga actgattctt tgaatgagcg tcctccgttg tttgtataaa
     5581 acaaactttc acgtgtcgaa acaaaaccta aagaagttgt aacgaagctg acattttgta
     5641 ctggctgatt gatcattgta cttccacttt ttatccaagt gattccgcca tccatagtag
     5701 tgtaaatgta caaagtttct gcagacatcc cactggctga tgtacagata aatagaccat
     5761 cgttgccata aaataccgct tttcgataac gtatcggctg aactgattgg gtgataatag
     5821 acgtttgcca tgttttacca ttatctcttg aagtcagcag acgtacatca tccccagaag
     5881 aatagataaa ccatgagaaa tcggcggaaa tatcatatgt gttgtccatc caataacctt
     5941 caggaatcag ccccgttgtc agggtgtatt cgccaaaacg caaccattgc gaatcaatcg
     6001 gcacaaatac ccaattagca ccactatctg ttgtcatata gagagagtgc gtctctgtat
     6061 caagtttgag atcatttatc ttttgtgttg tcaaatttac cttttgctta ggatcagcta
     6121 acaacccttc atttgtcttt tgtatatgcg tatcgttagt tgagttcatt gtcatgtgag
     6181 aagcagcatt tatatgagaa gacatttttg gctgagtgta tctcccataa tagattgttc
     6241 cagctaaaag aatgaggaaa aaaccagtga ctaatgtact catcttagtt gtcaggtatc
     6301 caccaggcga agactggctt ttttcccatc tgggttcgtt ttctagtaaa aaaatatgga
     6361 atgtgttcat tccgaatttt gatttgcttt cgcctaagta gataggtaaa tcatcaagtg
     6421 atctcagcaa taactggcaa tcactttttt gtatccgaaa agcttgactc gtcgaagtct
     6481 tctcaaaata aagatagtca tcatcttgag aagttggttt attatgtaaa aactgttgtg
     6541 ctctttgact aagccattga tcgaaatgac gcgacaattg atggtaacgg agccaaataa
     6601 gtagaagaaa aacaataagc acaacaattg ttattccgcc aactccccac gaaaagtgat
     6661 acaaattatt taatttttgc atcccttcag ctgaacggac tactgcactc atatggcgat
     6721 ataggaacga tacagctccg aaaaaaagca aaccagagaa aaatactaag cttaaaagct
     6781 tgaacattat tcttttcgct ctttttttcg ctttctcgat aattttttgt ttagctgata
     6841 atacttcgat aataaatagt tcttgatcca tttgtctctc cttttctcat acttcattca
     6901 ttatgcgtca aaatccatac aatcactagt cccactaata aaaaatttat gaatttggta
     6961 tgtactctca atttcaggta gtttatacat caaaaaatac taagattaat agacaaaaat
     7021 ttgatgatta cagagatctc ttacaagacc attcagaaga aaaagacatt gaacgtgttt
     7081 gagaacatga aaatcaaaac ctttttgatc aatcaattaa tcaatttgaa ataacaaaaa
     7141 atctgagaca gaatgaagtt cattctggcc cagatttgat tttgcacaac tcatcagtcc
     7201 ttattgacaa agagccaatt aattagcaac gatattgact aacttgtttg gtaccgcaat
     7261 aacttttctg actgttttgc cttcaatata ttcttgcact atttcattgg ctaatgctgc
     7321 tttttctagc tcatcttttc ctaagtctcg gggaacattg cttttcgcac gaactttacc
     7381 gtttacttgg aaaacaactt ctacttcgtc ttcaacaagt gctgcttcat cgtatgttgg
     7441 ccatggtgcg taagaaatac caccatcatt tccaaggatt gcccatagtt cttcacccat
     7501 gtgaggagca atcggtgcga gtagttgaac aaatccttcg atatattcat aaggcagagc
     7561 gtccacttta taggcttcat tgataaagac catcaattgg gagattgctg tattgaaatg
     7621 aagattttca tagtcttctg tcactttttt cactgtttga tgatacactt tatcaagctt
     7681 gccatcattc aatgtagtga tacggtcacg cattttattg ttctcatcaa cgatcaaacg
     7741 ccatacacga tccaagaatt tacggcttcc ttctaagcca ttttcgctcc aagcaatcga
     7801 agcatctaat ggccccatga acatttcgta catgcgcagt gtatctgcac cgtaagtttc
     7861 gaccacatca tcaggattga ctacattacc acgagatttt gacatttttt cgttgttttc
     7921 gcctaagatc attccttgat tgtatagttt ttggaatggt tcttttgtag gaacaacacc
     7981 gatatcgtat aagaatttat gccagaatcg tgcatatagt aaatgaagta ccgcatgttc
     8041 tgctccacca atgtagatgt caactggtag ccaacgcttc aacttttcat aatctgctag
     8101 ttcttttttg ttatgagggt cgatataacg caggtaatac catgagcttc ctgcccattg
     8161 tggcattgta ttcgtttcgc gacggccttt tttacctgtt acaggatcaa cgacattgac
     8221 ccactcctcg atatttgcta atggagattc tccagtaccg cttggcttga tgttttttgt
     8281 cttaggcaac cgtaatggta gctcttcttc tgggacagct gtgactgttc cgtcctccca
     8341 atggatgatt gggattggtt caccccagta acgttgacgg gagaatagcc agtcacgtaa
     8401 gcgatagctg acttcttttt tccctacgcc attttcttct agccattcat tcattttatc
     8461 gattgcttct tgtttgttca tgccgtctag aaattcagag ttgatgtgcg taccatcttc
     8521 tgtgtaagca gctttttcaa catctccacc ttcgataact ggaagaattt ctagtccgaa
     8581 agtttgagcg aattcaaagt cacgttcatc atgtgctgga acagccatga tggctcctgt
     8641 gccgtatgaa gataatacat aatcggcgat ccagattggg atttccttgc cattaactgg
     8701 attgattgca taagcaccag taaatactcc tgttttttct tttgcaagat ccgttctctt
     8761 tagatctgat tttttagctg tctcttcgat ataagcttct acagcagctt tttgttcagg
     8821 ggtcgtaatt tctctaacaa gatctagttc aggtgcaaga accgagtaag ttgcaccgaa
     8881 tagagtatct ggtcgtgtcg taaatacggt ataggattta tccgtaccag ccactttaaa
     8941 ttcaatattt gctcctactg aacgaccgat ccaatttctt tgcatttctt taatgctttc
     9001 tggccaatca actaattcta gatcatccaa taagcgatca gcataagcag tgattttcag
     9061 catccattgg cgcattggtt ttctgactac atcatagcca ccacgttcac ttttcccatc
     9121 gatcacttct tcatttgaaa taactgttcc taattcaggt acccagttta ccgctacttc
     9181 tgcttcataa gctagacctt tttcataaag cttcgtaaag atccattgtg tccatttata
     9241 atactccgga tcagttgtat tcacttcgcg attccagtca taactaaatc ctaaagaatt
     9301 gatttggcgt cggaatgttt cgatattttt ttgggtaaac tcagctggat cgtttcctgt
     9361 atctagcgca tattgttcag caggcagacc gaatgcatcc caccccattg gatgcagcac
     9421 gttgtatcct tgacttcttt tcacacgtga aaggatatcc gttgccgtat aaccttcagg
     9481 atgccctaca tgtagccctt gaccggatgg atagggaaac atatccagtg cgtaaaattt
     9541 tggcttttca ggatcatcat gggtgttgaa agtattcgtt tttgcccagt atttttgcca
     9601 ttttttctca atttctttat gattgtaact catttgctgt cctcctgttc tattgaaaac
     9661 acttgtttct attataaata taacgaaaaa accggcagaa tctgtttatt tttatttcct
     9721 tttgctttgt tttctcaaac ttcacaaaag aaataaaaaa atcctataaa aagattgctc
     9781 tttttatagg acgtaatcta cgcggtacca cctaatttta ccttagtgaa aaggcctcga
     9841 acgttgtaac ggccgtcacc gccgcttgct tactcgggta ttcagcaagg gcaactcaaa
     9901 ggcgagttca aaagcatccg tgcacattct caccagccat gcgctctctg aaacttcagt
     9961 ctttttacta cttccttttc aagtgttgtc atatttaagt agccttatag taacaaatat
    10021 ttttgtggaa ttcaatcgtt atttcttcga acagctattt tatttagcta caatcaacgt
    10081 ttgacctgga taaataaagt tgttcttaat attgttccat tcgattagtt gagccattgt
    10141 aattccatga ctgtcagcaa cactccaaac agattctcca gatttcacag tataggtttt
    10201 cgaaccagta actgattgtt ggtttgttga ttgagatgaa cctttcttca caatcacttg
    10261 ctgtcctgga tagatgaaat tatttttgat attattccat tcgattaact gatccatcgt
    10321 gatatgatgt ttgttcgcta cgctccaaac tgactcgccc gctttcacgg tatacatcgt
    10381 attaccagtg gatgatgtac ttgtcgtagc agacgtattc ggtgtatttg gtttcgctgc
    10441 agaagtagtt gaacctgtat ttgtatttgc cgaagttcca cctttaacga tcaattgctg
    10501 tcctggataa atgaagttgt ttttgatatt attccattcg attaactgat ccatcgtgat
    10561 gccgttttta tttgccacgc tccaaacaga ttcacctgct ttcacggtat aagtgccttg
    10621 agacgaactg ctgccagtat taactgaact gctgttattt gtattggttg acgaacctgt
    10681 attacctgac gtgctgtttg ttgatccact gtttgaaaca gtcagttgtt gtcctgggta
    10741 gataaagtta ttttggatat tgttccattg gattagctga gccatcgtaa ttccgtattt
    10801 atttgagacg ctccaaacag agtctccggc tttaactgta tatttcgttc ctgaagctgt
    10861 attagaagaa cttgagctgg acgaactaga gccacttgta ttcccagtat ttgagccagt
    10921 atttgctcca gcattagaag aaccgccatt tgatacgatt agctgttgtc ctggataaac
    10981 gaaattgttc ttgatattgt tccattcaat caattgagcc atcgtaattc cgtgatcatt
    11041 tgagatcttc caaacagaat caccagattg aacagtgtat ttcgcaccag ttgtttgtcc
    11101 tccgtttgat gtaccagcat ttcctgaaga attggtattg ttaccggaat tattcgtcga
    11161 agagccattt tcctggctac ctttgattgt cagtttttgt cctggataaa tgaaattgtt
    11221 ttggatgttg ttccattgga tcaattgatc cattgtgata ccgaatttat ttgagattcc
    11281 ccaaacagaa tcaccagatt ggacagtata agtggtcgaa gttgctgaac ctgagtttgt
    11341 attaccagaa ttgcttgaac cgccagtact acctgcactt ccagtattat tgccacctgt
    11401 agtcgctcca ccgcctgcgt ttccgctagc tggtgtatca tattgggtca atccgtaagt
    11461 tgcgatgata ttatttaatt tgcctgcgta accaggatca gtcgcataac gccctgtcaa
    11521 acaagctgtc gcatctgtat aggatttcgt gttgctcttc caagtaccgg catagtaata
    11581 accgttgcca aatgaagtat ttctaagtac ataggcattg tcattgaaag attcagcaaa
    11641 agaaggatat tgtcggaaag gctccttttt atttacccat tgattattta aaaattcaag
    11701 cgtatccatg tagacggttt gtccttgata gctgcctttt atcccaaaaa gattataata
    11761 aggtgcttga gataacgcac tactgcccca accgctttct acaactgctt gtgcgatcat
    11821 cactgatgca tatagatcgt tggcttgagc gattggttga gcataagtag caatttctgt
    11881 gataaatg
//
LOCUS       DACEPW010000075        11680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEPW010000075 DACEPW010000000
VERSION     DACEPW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.076"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..1004)
                     /locus_tag="IW819_001583"
     CDS             complement(93..1004)
                     /locus_tag="IW819_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1244692.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1405..1983)
                     /locus_tag="IW819_001584"
     CDS             complement(1405..1983)
                     /locus_tag="IW819_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1244693.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(2003..2671)
                     /locus_tag="IW819_001585"
     CDS             complement(2003..2671)
                     /locus_tag="IW819_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244694.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(2686..3756)
                     /locus_tag="IW819_001586"
     CDS             complement(2686..3756)
                     /locus_tag="IW819_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1244695.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(3927..5291)
                     /locus_tag="IW819_001587"
     CDS             complement(3927..5291)
                     /locus_tag="IW819_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1244696.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(5288..6847)
                     /locus_tag="IW819_001588"
     CDS             complement(5288..6847)
                     /locus_tag="IW819_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1244697.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(6903..9383)
                     /locus_tag="IW819_001589"
     CDS             complement(6903..9383)
                     /locus_tag="IW819_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244698.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(9367..9702)
                     /locus_tag="IW819_001590"
     CDS             complement(9367..9702)
                     /locus_tag="IW819_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244699.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(9716..10750)
                     /locus_tag="IW819_001591"
     CDS             complement(9716..10750)
                     /locus_tag="IW819_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1244700.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(10840..11512)
                     /locus_tag="IW819_001592"
                     /pseudo
     CDS             complement(10840..11512)
                     /locus_tag="IW819_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
BASE COUNT         3346 a         2370 c         2025 g         3939 t
ORIGIN      
        1 cttaaacggt gtttaaaagc ttgcatctgc gataataagc ccggccaaac gaaaaatatg
       61 aggagctatt tttgtttggc tgggcttatt tattactgaa aataagtggt caatgcttgt
      121 gtcaaacttg cagcaatctg ctgctgataa ctgtctgtta ctaattcctt taaatcacta
      181 tcattattca tataacccat ctcgattaaa atagaaggtt gaagattttc tcgaagaact
      241 tgatagttcc cagtggagat tcctcttgaa tccagaggca gatcagtgaa ggagtcagca
      301 attgtctgtg ctaatgtttc ctcttgtcca tagtaataat atgtcgtgat tccagttgct
      361 tcattcgcat attccgtaga gtctgcgtgt aaactgataa agacatctgc ctttttttct
      421 tcactgtagt cgcatatatc tccaagctgt actaattcat ctgtagtatg ggtcaaataa
      481 accgttgctc cagcatcctc tagcgccttt tttactttta tggctgtact taatgttata
      541 tctttttctt ctgtttgttc atcattgctt aatgccccag tatcatttcc gccatgccca
      601 ggatctaata caatcactgc atcagatagc tcttccggtt ttgtttgtat attctttgtg
      661 actttggttc catcttcgag ggctaaatat ccagtatagc catcatttgt cttgatctga
      721 taagcatttt ttgtttcaga aagaaactcc gccagctctc cgccgtttat agttgcaagt
      781 ggggtagtta gcgagtcatc tgtatatagt gtacggtcat tttttccgat tttgattaat
      841 gtctcttgtt ctgtatcatc aaatgattcc tttgtctttg taaccgtact gttcgtactg
      901 ctgaccgttt gtccaactat ctttttttga ccaaaaacat ctatttgaaa acagataata
      961 atgataatga ttgcaaatac aaaagctcca attatttttt tcattcttga ctctcacctc
     1021 gtagatccag actaagggcc aaaacttaaa atcagcttaa gtaagcaaaa aaggggctgt
     1081 aaaagatata accgtagaaa ataacattac tgctaaaatg atttttttca tttttggcat
     1141 atcctgaaat ctccttttga ctattcgtgg tgattcatct acccaataaa tagtttcctt
     1201 cattctacaa acaattaatt tcttcgttat taaattaaaa cacttcctgg aacaccgaca
     1261 taataaataa aaatataccc tgataatcat gagagaataa caaatttgta tttatgaaaa
     1321 actacctata taaagaagca agatataacg atcttcgtca tatcttgctt cttcacctaa
     1381 ctattttctc cctatataac agctttacgc ttctcctaat acaccattca aatataaatc
     1441 gatcaattgc gccacattta gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg
     1501 tgctaacact aagaaaaaat gccttgctgc gatttctttt gaaatcccat ttcttagcac
     1561 agattggttt gctgaaaaga tttcttcaaa aggttcacga taatttttct tccatagaaa
     1621 gaaaagatga tctcttgttt cctttgaaag attgctttca agatcgtgaa gcatcagaaa
     1681 caaatcgacc agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa
     1741 tcccgtttcg cttgtttgaa cgatttctct caaagctttt ccaacatctt ttaagtggtt
     1801 ttctaaaact gccgtatata gtttttcttt atcaccgaaa taatgataga gattgggctg
     1861 ggtgatccca actttttgtg cgatttctct tgttgaagtg ccgagatatc ccttggacat
     1921 aaacaagtca gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt atcgatggac
     1981 catttttcca cctgcttttt ggttattctt tttctttcaa tacaccatct tccatcataa
     2041 atattctatc gcatttgtca atcagccgtg tatcgtgagt aaccatgatc gttgctttat
     2101 ttttgtcttt tgattctttt gccaagatat cgactacttc tcgtgccttt tctgaatcca
     2161 aactagcagt tggttcatct gctagaataa tggacggatc attgtacaat gcacgagcga
     2221 ttgcgaccct ttgccgttct ccaccagata gttcttctgg atacttgttg atcaacttat
     2281 ccacgccaag ctgctcaaat aattcgtttg cttctttttg acgatgttca tgtttaatct
     2341 tatctactaa taataactgc ttttttactg tcagaaaagg aaccagattg gaagcttgca
     2401 aaataaagcc aatttcttta aaacgtagtt taacgcgttc gctttctttt tgattactga
     2461 attcttgatc attgataaat acagagccac tgctaggcgt ctgcaatccg cctgccaaag
     2521 tcaaaaaagt acttttacct gaaccagaag gaccgatgat tgcaacaaat tcccctttat
     2581 cgatactcat ggttgtttct ttcaatgctt taactacttg gtcgccatct tggaaacttt
     2641 tttctacttc tttaaattct attgctttca tgttcttcct ccttcttatc caattgcctt
     2701 caacggatcg atttttacga ttgtccgaac agaaaatagt gcgcctaaca cagctactac
     2761 taacatcaac aagctaatga cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc
     2821 aggtagaaca agggatgttc ccactgttcc taatagacca agaagaatac caacaaatga
     2881 taaaatgaat gtttgtgcca ctacagaacg cgcaataaat cctcctgtga tcccttgtgc
     2941 tttcattaca ccaaagatat taatcttttg catcgtcagt acataaatga aaatcccgat
     3001 cacaatagcc gcaataacga tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc
     3061 attatatcca ggtaattcat tgatgaattt agaaattttg atctgttcta aatcttcagg
     3121 taaattactg attttacctc tgacaactat tgcgttaatc cgtgcatttt cacttgtatc
     3181 ttctttttcg aagcggattt cttggtaagc attgatggtt gtatagagaa ctggtgagac
     3241 attgaatttg gcatgatccg taaagccagt tagtttaaac gtcttgtcac taccagataa
     3301 cttaactgta tctcctatgg ctagaccata ttcttctttc aaactaatat cgcctactgc
     3361 ctcatcatcg ttatcaaaag cttttccttc aacaagattg ggcatgatga attgattttt
     3421 atcgataccg aagaagctaa cattgatttt tccttcttct gtactgtctt tactagtcac
     3481 gactccaggt gtttgggcaa gatacgccac tttcccacct tcgacttctt ctgctatctt
     3541 cttagtaatc atggacatcc ctagatttga atttgcatct ttcgaaagca caatcgaatc
     3601 tgcctcccac ttgtcgactg ctgttcgatt atcttgagct aagccataag ctaacccagt
     3661 caaaaaaaat accaggtagg cgatcaaaaa catcacccct gcaactaacg cataacgtaa
     3721 ctttgaatgc ataatttcat ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc
     3781 attttataca acaattttat cacttgataa atttttggcg aatattatgc tcaaaaaatt
     3841 cttccgctat ttcattatat gaatttttta ttctcgattg attaatattt attatatccc
     3901 cacacaataa aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct
     3961 ctcttagcta gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtattca
     4021 gacagaaatc caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata
     4081 tccgcaagca ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt
     4141 atttctaatg tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcgtcg
     4201 tattgtggaa atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc
     4261 gcttcatctt ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt
     4321 atcttttgtc tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt
     4381 gccatatgta cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa
     4441 ctgactggga cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca
     4501 ttgactatca cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta
     4561 ggacgagttt gggcagaaaa aatgaccata tctgccttta ttgtttgaga cagaagttgt
     4621 acggatacta ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa
     4681 tttatttgct tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat
     4741 cttgataaaa cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa
     4801 gcatctgcta attccaatcc aatgtaacca gcaccgaaga tcactacttc ttttgcttct
     4861 tttaaagcgg caagtacttg cttagattgc actagatttt tataggtgta gacattttca
     4921 gcttcaattt gtttatacaa ggtgacagga gaggaacctg tcgctacgat taatttatca
     4981 taggttatgg tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga
     5041 tcaagttcag ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc
     5101 atgttatggg caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca
     5161 tttgaaacat agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc
     5221 ggttgaagtt ttcttagatt tagggcagct gtgatgcctt catgggaagc accaatgatc
     5281 acgattttca tgttttattc atccttaaat tcaatttttc taaataacat tcttttaaaa
     5341 attggttcaa ataggggaaa tcataattat attcgttgct agtgaataca aaaactttag
     5401 cggacttctg tgacgggtac tctttcaatt ggctagtcaa aatgatatca tactgctttt
     5461 ctttctcgta ttcgactaaa tcatatttga tcgtattcag caattgttcc tggatgattt
     5521 ctttcacgat atccgccatt agcctttcat aaggaagatc ataagcaatt tgcagacgta
     5581 attctaactg cttgatcagc gtccgtaaaa tagatgcata gcgagcaaat aattcaaagc
     5641 gggcattttc ctcgtctact tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc
     5701 tttcacataa aaccaggaat tgtttatctt ctacttcagt taaccgctgt tttaatcctt
     5761 cttggccaaa aatattcatc catcctctaa aataggcaaa atgagcgtga ttttgcatca
     5821 ctgtcaacgc aatcttttgc gaaatctctt ctggaaaaac ataaaaagag aaatattctt
     5881 ctatcgattg tttaaacgtt cgattcaatt gagctactac gtcgtctttg tatttttcgc
     5941 ttatcaaaaa ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga
     6001 aaagataaaa aaccaacgtt tcattttgat tccaaggaaa ggcatattga aagaagtaac
     6061 gggtcaaaaa tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa
     6121 gattgctata atctggtttg gattttttcg tacagcgcat tcgcttcttc ataatggaaa
     6181 gccatatttc aaatttcaat cgtccttctt ctgtaatagc aaaactaaac tctttttgca
     6241 gcagatctaa aaaagtgttt cctgataaat cactgtattc ttttttgttt tcaagatagg
     6301 gacgtccaaa ccaaaataat tggaaataaa aataacgaat ttgactttct ggaccggtca
     6361 gccgcccgct ttttattttc agatgaaact catttaaaga atcatttaat tccttcactc
     6421 tgcgagacaa agtagccgaa ctgatattaa gttccatgac aagtttctgc cgatcaaatg
     6481 agccattttc caacagataa agaagtatct gatattttgt agaatagcgc aagtaacaat
     6541 gataataaac atcgactgct gctatatttc catgtctgat taataaaata ttcccttctt
     6601 ggataacaat ttccaattgt ttgatatttt ccaagttagc ttgtaaataa aacagatctt
     6661 ctttcaaggt agcccgatca actgataagc gggtaagtac gttattaacg gaaacctctc
     6721 ctttttcctc taataaaagt tggaaaagtt tgatttgccg ttgttcattt ttttcaagca
     6781 aactgaaaag ttccaagtcc actccttcaa gcatttctga ttttaacagc agaagtttcc
     6841 ttctcatcct tgaaaaagag gatctgtgac agttatcaca gatccataca aattctaatc
     6901 acttagctgg ttggcccagc tgtcagttcc catgtgacca tgccttgata cgttgcgcca
     6961 ggttgagcat tcgtactgct atttacctct aagagtaaac catcggtatc gggaaagtca
     7021 acgagaacct ctcctttagc tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc
     7081 tgaatcaatt gctttgatcc agaatgattg aagtataatt ttgttccgct taattcttga
     7141 ccttgatcat tcttaaataa agaagtcaat gtacctgtca gttgccaagg agacgtgctt
     7201 ggaagccgag tatcccgcac atcaaaagaa aaatcgcctt ttgattcata ataacttgtc
     7261 ttcaacggtt tggggagatc gttaccaaat gtaaaatcat ctggtacatt aactaatgtt
     7321 aattcttgtt tggggacaag aattgtttta gcagatgttt ggacaactgg gttaggaagc
     7381 ttagcaccat ctgctaatgt aatattgctg gaaagtacag ctgcctttgt cagtatgctg
     7441 acggattgta aagaagtatc tacagtattt aattgatagt tgtaagtaag atgaatggta
     7501 tctcctattt tgctcaatag atctgctggg acagaaactc cttgttgaat gccggtatct
     7561 gcgatagttg taccatttaa ttttaaggaa ccattgatat aagtagtatt ttcaggaagt
     7621 gagatcgtga tttttttgtc ggttatcgca tccccagatt ttgttaacgt gaatgtcaca
     7681 ttttcttgaa tcgtatctcc tgatagagca gacaggggat ctcctgtctc aggttgtctg
     7741 ctaaggtttt ttagctgact atctgcactc acagatggaa aattagccac tgttatttga
     7801 ggaagagtgg tgattccagc ttgttcagac tgccatttgt acgcaaaaga cggatacatg
     7861 ttagtaagta catcgtccac caagcgataa tcaaaataat attgccctga agccaatgca
     7921 ggtagtgtag aagtagctgt atttacaccc ccgttatttg attggatcgt ttggaaaagt
     7981 tgatctgtcc atcctgtcgc taatttttgg gttcctttcg aataaagcct gtaatggatc
     8041 gaaccctttt ttagttcagt tgctgaccaa ttggccgtga cttggtacga tgcagaagaa
     8101 aggagctttc cccactgact atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc
     8161 ttgtactgct ccaatacctt taaagcaaca ggagaaagca atccgccttg cgcatctttc
     8221 ccactgtaca cgatattggc tgtctgattc aatccacttg tgcttgctgg agtatgagtc
     8281 agtattgctc cttgtgtaac gttttttcct gtaaaatagt tgactacgcg atcaaagcga
     8341 ctaaatggca actgatcaaa acgtccttca gtcagcccca tcgtcaatgt ccgactattg
     8401 gtagagcctg gaaggttctg gttatttcca tccacgtatt ctgtggtatc agcaaatgta
     8461 ttatattttc ctgttgaatc acttggtgag atgtaactat tccacgtatt tatctctgca
     8521 cttgcagaaa atgcatatgc tcctggattc aagtttccgc cagttaattt aggataaaat
     8581 ctacctgcag ctggagctaa aatcgaattg aacatacgat cttggataat attattcgaa
     8641 tcataaaaat caacagaaaa aacggccttt ttagacatag aaggttgtaa tttacccttg
     8701 gtcaaaattt tgtttggact cgtatcatat ttatctccgt tttctgtcat cttcgcgaca
     8761 ttagctgtaa catcaaattg aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata
     8821 atagcaataa tatctgtcaa tagagtattt gtatcccacg accccatccc aaaacgcaag
     8881 gtatggcttc ctgctgataa taaacttccg ctagtaacct tgatgggggc accggcaata
     8941 tacaagtagg cgttgttcca gtcaattgct tttaatacat cactgcttga tacatgttcc
     9001 ggcaaattga tgatcgccac aaatgcaaat ccttgtgcag tgatattcac caaatcaagt
     9061 acatttgtta gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc tttccaagga
     9121 tcatttgaac catcgttcaa ataagtagca gtcaatttag gcaattggtt agccccagtt
     9181 agttggacaa gcttcccgcc attgactgca ctacgtccac catttggagg tacgatattt
     9241 tcatacgtgt atggatcata agaaatggca ggaggcgttg ctccaacagc ttctgccgaa
     9301 ggtgaaaaga taaatatact taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat
     9361 gaaaatttat tttgtagcaa catgttcatc ctccttttcc tttcttctcc ataacaacaa
     9421 ccacgcaaga agccataatt ctataccaat gatcaaataa agaaaacgga tcttttgttc
     9481 attcgtctcc gggaatacgc catgttgatt agattcttta taaatcggta gctgtgtctg
     9541 ctgttttgat gcgccattga ctgaaccact tggatgcgga attggactta gatcataaaa
     9601 tgtaatcgta cctgaacttt tggtatctgc ttggactggc tcttgccagc tgaagaacag
     9661 caaacttgca ataatccctc ccaataagat cgatgttttc aacgaaatca cctcatcatg
     9721 ttgtttctcc ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca
     9781 ctagaactgc agcaaccaat cctgcataaa gccatgggaa agattgttct tgtactgcaa
     9841 aaacggaatt ttcatttact ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa
     9901 atcgccattc attactgtcg cctgattttg ctatgatttc tgcgcggtaa tcgccggctt
     9961 ttgctttatc accagctaaa tcaagattca agtgaaaatt cgaatttggt gcaactaaaa
    10021 atgcattaga cgtattttca atataaagct gattttcata aaagatcttg ctcgtaaata
    10081 tcaaattgct gataatccta ggctgaggat tttggaaacg aagctgaaca gttggctttg
    10141 cgttgacctc gtccaaatct cctgataaaa gtttcagatc cggttctatc gtctcttgac
    10201 tttcttgtaa aacgactgcg agattataag caatacgatt tttgatttgt gctccttctg
    10261 tagcattttc tgtttcagct tcttctttag gtgaaacatg aatacccgct aatacacgtc
    10321 ccttaaagct ttttgctggg attttcactt ctataggtac agttgtttct gcatgtgctg
    10381 atacattgat ttcctttttc gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct
    10441 catctaacgt tgggtttttt tgactataat cgatcgttcc accatcactc gttaccgccg
    10501 gattgacaga gacctcaaat gttcgttctt catctgaaga attgctgata attacttcta
    10561 gtgtctgatt ttgttccggt gccactaata aatcaaaata accgattgct ttatctattt
    10621 ggttctctgg aaataccggt ttgattccaa agtctcctgc tgaatccttt gcttggctga
    10681 ttgacggaaa aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac
    10741 tttgattcat tgtccaccac atcctttcaa caacaactgc ggtaaaaaat ccttcaccta
    10801 tagtattttc accgcagttt gttgaattta tttcatactc tatgctactg ggccagccac
    10861 gaggttccaa gtcaaatcag ccgtgaatgt tccagctgct gctttcggca ctttcagtgt
    10921 gatatttgct ggatcataaa cagaaagcca tgttcccatc ttttgctgct gtaaatattg
    10981 gtgtcgctgc acttgcttta ctcaacccag ttacagtttg actagttggt ccagtggatt
    11041 cgttatctga cgtactttta acagtggatg ttccaaaatc taattcagcg ccttgcaaaa
    11101 cttgtgaacc gtttacaaaa tctgaaaccg aaactgtcac gttccaccct tgtgcttggc
    11161 catctgcacc tacacctcga cgatctgata cttggagata aggtgtatcg ttttttgaag
    11221 cgtctacttg atacgttttt gttccgcttt caatctcgtg tgtaccaaat ggcaattcgg
    11281 gaactacatc cagtgttaaa ggacctgtct ggccagttgc tggattttcc ggatctgctg
    11341 gctctactgg ttttgaagga tcagtagggt taagaggctc cacggtattt gttggttcag
    11401 taaaggtaat atctcctttt gaagtcgctt gaccctcagc aaaagctggt gttgccaagc
    11461 cgcctagaag aagtgttgaa agtaataatg tcccaatcat gttctttttc ataataaatc
    11521 actccttttt cttagtgacc taagtatagt agagacagta tgcaaaaaat cctcatgaat
    11581 tcatccgctt acttgaaatt atgacccttt ttgtataaaa gagaaaaaaa caataaaatc
    11641 gtttacatga atattcttcc ttttttatta agtttcgttt
//
LOCUS       DACEPW010000076        11590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEPW010000076 DACEPW010000000
VERSION     DACEPW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.077"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..970
                     /locus_tag="IW819_001593"
     CDS             107..970
                     /locus_tag="IW819_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1244701.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IW819_001594"
     CDS             complement(1099..2721)
                     /locus_tag="IW819_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1244702.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IW819_001595"
     CDS             2970..3491
                     /locus_tag="IW819_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244703.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW819_001596"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW819_001596"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1244704.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNVSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IW819_001597"
     CDS             5572..6390
                     /locus_tag="IW819_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1244705.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IW819_001598"
     CDS             complement(6493..6690)
                     /locus_tag="IW819_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244706.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IW819_001599"
     CDS             6859..7437
                     /locus_tag="IW819_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1244707.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8481..8984)
                     /locus_tag="IW819_001600"
     CDS             complement(8481..8984)
                     /locus_tag="IW819_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1244708.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9343..10368
                     /locus_tag="IW819_001601"
     CDS             9343..10368
                     /locus_tag="IW819_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1244709.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10691..11569
                     /locus_tag="IW819_001602"
     CDS             10691..11569
                     /locus_tag="IW819_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244710.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3589 a         1973 c         2257 g         3771 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaaacat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacact
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aacccttttt gttaaataag gggaatatag cctgtcagtt
     8341 tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa atagattttt
     8401 ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt gtctattacg
     8461 gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa taaaaaagct
     8521 acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc taacttattt
     8581 gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc tcctgttgta
     8641 ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg aatcaattct
     8701 gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag tctcttgaaa
     8761 ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt ggtctcacgt
     8821 aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac attgctgtaa
     8881 gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa ccataataga
     8941 aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat ctcctccatt
     9001 taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag tttaaggacc
     9061 aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg tttctttaac
     9121 aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca aaacgactcg
     9181 gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac taagtttatg
     9241 aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg gcatttcatc
     9301 ttggttcagc tttttttcca attactagga aggagggata gattgttcaa gacttatttt
     9361 cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct acttatcttt
     9421 attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt ttcatcatta
     9481 gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct gaaacgtatg
     9541 tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc gacttatgtt
     9601 ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt cgtgttgcga
     9661 ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc catgggaacg
     9721 agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt tatgggtgtc
     9781 gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc tgagcagaca
     9841 attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat cattgattct
     9901 gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt gaatctgcat
     9961 aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg
    10021 atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg tgtgtcggca
    10081 ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac ttttctatat
    10141 ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt tcttaatccg
    10201 atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt gatcgaatat
    10261 cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg
    10321 gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat
    10381 atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa
    10441 agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa ctttcaaaaa
    10501 aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat aaactttcct
    10561 gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata gaaagtttaa
    10621 gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg gataatcagt
    10681 catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga aaataagcaa
    10741 tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag agcaaatgat
    10801 tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg aactaatgga
    10861 tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag aggaaccttt
    10921 tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa tttatcaacc
    10981 attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta gatttattca
    11041 tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga tttggcatta
    11101 cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat attttccgaa
    11161 agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata ttccttccga
    11221 tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc gtttttttga
    11281 actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt tactatcacc
    11341 caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag ccgattatcg
    11401 attattatca gttgatgaac aggacgatag tggagtccct tggatctttg gacgagtaga
    11461 tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa aagagcaatt
    11521 gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag tttagtactt
    11581 cgaaatagtt
//
LOCUS       DACEPW010000077        11539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEPW010000077 DACEPW010000000
VERSION     DACEPW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.078"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..812)
                     /locus_tag="IW819_001603"
     CDS             complement(<1..812)
                     /locus_tag="IW819_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1244711.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFG"
     gene            complement(858..1637)
                     /locus_tag="IW819_001604"
     CDS             complement(858..1637)
                     /locus_tag="IW819_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244712.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKERVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
     gene            complement(2022..4097)
                     /locus_tag="IW819_001605"
     CDS             complement(2022..4097)
                     /locus_tag="IW819_001605"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1244713.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(4099..5016)
                     /gene="glyQ"
                     /locus_tag="IW819_001606"
     CDS             complement(4099..5016)
                     /gene="glyQ"
                     /locus_tag="IW819_001606"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1244714.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(5402..6214)
                     /gene="recO"
                     /locus_tag="IW819_001607"
     CDS             complement(5402..6214)
                     /gene="recO"
                     /locus_tag="IW819_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1244715.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(6357..7256)
                     /locus_tag="IW819_001608"
     CDS             complement(6357..7256)
                     /locus_tag="IW819_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1244716.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(7271..7672)
                     /locus_tag="IW819_001609"
     CDS             complement(7271..7672)
                     /locus_tag="IW819_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1244717.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(7650..8126)
                     /gene="ybeY"
                     /locus_tag="IW819_001610"
     CDS             complement(7650..8126)
                     /gene="ybeY"
                     /locus_tag="IW819_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1244718.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(8155..10347)
                     /locus_tag="IW819_001611"
     CDS             complement(8155..10347)
                     /locus_tag="IW819_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1244719.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(10371..11342)
                     /locus_tag="IW819_001612"
     CDS             complement(10371..11342)
                     /locus_tag="IW819_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1244720.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
BASE COUNT         3091 a         2485 c         2064 g         3899 t
ORIGIN      
        1 ccaaatgcct cccgataatc taaagcgatt tttttcattt catcaaagct catgtctgtt
       61 ttcatattcg attctagagc attcaaaacg gattgatagt tgctgacacc gtttagactc
      121 aatacttttt tcgctacacc ttctacgatc tttctttgac gctcttgtct accatagtca
      181 ccatttggat cttcataacg catacgagaa taagataatg cctgatcccc gttcaatgtg
      241 attttaccga ttgtaaagtc atagccatct tgtgagaaag tgaggtcatt attgacttcg
      301 ataccaccaa ctgcatcaac aagttctttt agccctttca tattgataga gacatagtga
      361 tcgattggga tgtctacata tttttgtacg gtatccatcg ccatggcagc tccgccgaat
      421 gcgtaagcgt ggttgatctt gtcttttgtg tcacgaccta cgatgtcgac atacgtatct
      481 cgaggaatgc tgacgatcgt tgtctgtttg tcttcaggac tgactgtggc aaccatcatc
      541 gtatctgatc ttccttgatc gacccgacct aaatctccag tatcaatccc catcaataga
      601 acagagaaag attcttgttt gtccagatca actggtttcg ttcgttctcc ttgggttcgt
      661 tccactgatt cgtaagtttt ctcaatagaa tgtgagagat ccagatataa ttttgctcct
      721 actcctacaa ttgctaatac taatacgata agaactgcta aaataatcag aaatattttt
      781 ttccctcttg attttcttct tcgtcttgcc atagtttcct ccgtttgcta accttgcttg
      841 aatcatcgct ttaactgtca atagtttata ttgctatagc ccatttgctg agcaacagtt
      901 tgtgcttttg ttgcgtatgc tacgcttatt ttttcattcc ggatctgctc tatttgactg
      961 agcgccgtca aatattgatc gtaagtagcg gtggcagtca cttgaccatc ctttaacatc
     1021 gtatcgaata actgatctag ttgcgtgact tgcgcgatct gggtatcagc gaatcctaga
     1081 tattgcataa caaccgtttt ccaaggacta tctttaaaaa agctcatatt ttcacgtaca
     1141 cgagcgacat ctgcacttgt caccctttct ttgatgatga catcgtctac agcttgctgc
     1201 cagtttgata catttttagt aaataaatcg ttgacagctg tctggatctt cagttttgat
     1261 tctgcactat ctaactgtgt cacgattgct ttcttctcat cttggattgc aacagcttct
     1321 ttaggcaatt ctgattcttt gatgtcgtaa tcatctgctg aaagtttgac agaggatagc
     1381 ttatttgaca aacttgtgat tgtttcttct gttatttcag ggtttagaaa aattttttct
     1441 tttgtactat acagcttgtc tacttctttt gataaagaag tcaaggtgtc tttttgattg
     1501 gtgatggcag attctgcata attgatccgt tgctgatgtt gccgctgatt attgatatgg
     1561 tatccaaaca aacagatcgc aatagcacct gcacctaccg ccacagcagt caatgacttg
     1621 acgaatggct tttccataga ttcgctccta agtattgatg atagataaat taagatataa
     1681 ttcacttaca taaaaatgac agttttttat ttaaaaaaac ataattaaac taatatattc
     1741 acagaaatat ttgtaaacaa gttttcccta tataatcata ccacaaactg ctttcttgac
     1801 tagggaaatt tacgatttaa aagtaaataa aacattaata agataacaat taatcgattt
     1861 tacttttatt tccttaattt tcataggtgt ttttaaaagt tacacaaaaa ataaaataac
     1921 aaaaataatt aacattgaat taataatagt tatttataac aaataaacca gagaaatagc
     1981 ctattttgtg cctgttctct ggtttattta catattagaa attattttgt gatgatttga
     2041 tctaaacttg ctagtgacaa agccatggca gcaatttgac ttaattgcgt taatcggttc
     2101 ttacgtactg ctggttcttc tgccatgacc atcgtctgat caaaataatt ttcgatcagt
     2161 gggcgaaggc ttacaagctg atcataattt tcgttgatcg tttggtcagc aaagcttgtt
     2221 ttcattgcct ctactgcttt gtgcagttct ttctccgctt cgttttcgaa cagttcaggg
     2281 tctacttctg attgtttttc aagctccgct tttttcgcta agttcacgac acgtgtcaaa
     2341 gcttccatcg atggtttgaa gtcttcatct gcaagatgct gcttgaacaa ttgtgctgct
     2401 gcaaatactt ttgtcagatc tttttgttct gcattcaaaa cagcttcgat gacatcatga
     2461 cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacctgt
     2521 tgttgtcccg ctgacaagcc aatgccaaaa cgatccgcat ctttactgat aacttcacta
     2581 atcgtttctt gcagaacaga taaaggaaag gcccattttt tcgattcgat gattcgaacg
     2641 acaccatatg cttgacggcg caaagcatat ggatcgtttg agccacttgg gatcatccca
     2701 actgcaaaga aagaaaagat actatctaat ttatcagcaa tcgctaaaac agaacctact
     2761 gtagtctcag gtagatctcc ttcactggat ataggcatgt aatgttcacg gattgctgtt
     2821 gctacggctg gtttctcgcc tttaagcaga gcatattttt ctcccatgat cccttgtagt
     2881 tcagggaatt ctcctaccat gttggttact agatcaaatt tatagatttc agcagcgcgt
     2941 tgcagatcca tcaattcttc ctcagatagt cctacttgct cgcctatgat gcgggcgatg
     3001 acttgtaccc gttgcatttt ctcgtagatg ctaccgattt tttcatggta agtaacagtt
     3061 tttaattttt caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc
     3121 gcatcttcta aacgagcagt caatactttt tgattgcctc gaaccacatt ttccaatttt
     3181 acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg atcattccgt
     3241 acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa
     3301 agatattttt cttcgtagtc gccagaaaag acagttggat attcgactaa attgttgact
     3361 tcttctagta aatcttcgtc tagatcaata acccagtgat ttttttctgc taattcgttt
     3421 gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa ttgttcattc
     3481 aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga
     3541 cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac ttcatcatct
     3601 aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc
     3661 atcgttacag ggaaagtcag gcttttaatg acatctgcta aacctgtcaa tacttctttg
     3721 gattccttac catgagtgta tttcgttaca tagacatatt ccacaccttt taattctcta
     3781 aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc tgctttcgtc
     3841 cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg
     3901 tccgcttgtc gttctggaat atttgttact ttgattgcta aacggcgcgg tgtggaaaat
     3961 gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct
     4021 aattgtttca tgcttggtgt gacaacatgg gcaggcattt cttccagccc gatttcaagt
     4081 aataagtctt ttgccatctt attcagcctc cttcacggtt tctggatttg ctgtgttttt
     4141 attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg
     4201 agccatgttg cggatacggg aaagataacc ggcacgctct gttacggata ctgcaccgcg
     4261 agcatctaat aaattgaatg tatggctgca tttcaaaata taatcatacg ctggatgaac
     4321 aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag
     4381 caagtcttga tcgcttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa
     4441 gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt caacttcttg
     4501 gatataagaa gcaagacgtt ctagtccata agtgatctca gaagtgactg gtttacaagg
     4561 cagtccacca acttgctgga agtacgtgaa ttgtgtgatt tccattccgt caagccatac
     4621 ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt caacaaaacg
     4681 gatgtcgtgt tctaatggat cgattcctaa aaggcgcaag ctttctaaat aaagctcttg
     4741 gatattctca ggtgaaggtt tcatcacgac ttggaattga tgatgctgat acaaacggtt
     4801 tgggttttca ccataacgtc catcagctgg tcggcgagat ggttctacgt aagcagcatt
     4861 ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt
     4921 ttctgtgtca tacgcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt
     4981 taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttaa
     5041 agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct atgtctgatt
     5101 acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag attcggcagc
     5161 ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc
     5221 ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc
     5281 agttttcttg tttctataat agatcctctc actgaacgat ctatttgatc tacggttaat
     5341 aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta tgtttcattt
     5401 actattcttc ttttttaggt agatcttttt cagacatgtc tttctctggc gtctttaata
     5461 cgtgtgccca agacttcatt tgatcgatga actttttgct tttcaaatga ataccgacat
     5521 attcatcata cagctggtcg atgagttcac ggattgcttg tttggtttct tctttcagat
     5581 ggattccact gattttatca taagaaatag ctgaaaacat ccgaacgaaa taaacagctc
     5641 gaggatctgc atgataacgg tgatcgtcca tcgaccaatg tctttcgcat atcacaccac
     5701 catatttgga agaatagtca aattttcctt ttgtttcccc acatacgacg cagtgtgtcc
     5761 agattggtgc aattccgaag cgttgcagta tttgaacttc aaagatattc gtgatgattt
     5821 ctgcatcatc tccttgatcc atccgctgca gagcttgttg cgtaaattgg aataaatgcg
     5881 ggtcatattg atgatcttcg attgctgcat ctactagatt tagcaaatag gtaccataac
     5941 cgctcaaaaa gatatcttct tggatgttcc gaaacgcatg gacgtctttg acactgctta
     6001 aaaacgacaa accttcactt cgaaaattac cgatatagac tgcttcggta aaggggagca
     6061 aagccggagc taatggctga ttttgccgat ggattccttt gatataaaac atcattttgc
     6121 cagaagattc cgtaaagatt ttgactaatt tatctttttc tttgtaatta cggctagata
     6181 aaatcaagcc tttcgattct gcaagctggc tcatattctc cctcctaaaa aataaatctg
     6241 gaaaagcaga ttctgccgat ccagattatt tatgtttcga tcttccgccg tttttctcaa
     6301 cgtgcggatt tttatccagt ttatttctat acttaaccta taagaagttc agaatatcag
     6361 tattcatctt ttcggtagcc gaagtcttgc agatagactt ttttgtctcg ccagtctttt
     6421 tggactttga cccaaagttc tagaaatact ttgtctccta aaagattttc gatatccttt
     6481 cttgctttcg ttcctacttg cttcagcatt tttccgcctt tgccgataat gattcctttt
     6541 tggctatctc tttcgacgat gatcgtggcc tggatatgga ttttatcatt ctcgtttcgc
     6601 ttcatcgaat caacaacgac tgcgacagaa tgaggcactt cgtctcgtgt taaaaacaag
     6661 actttctcac ggatcaattc agaaacgatg aagtattcag gatgatcagt cacttgatca
     6721 tctggaaaat attgtgggcc ctctggcatt tcactgacta atacatccat caaacgttcg
     6781 acgttgtttc cttctgtagc agaaattgga atcacttgtg caaagggcat ctgcgttgaa
     6841 taatcttcga tgattcccaa caactcgtct ggatggattt tatcgatttt attgataatc
     6901 agataaacag gagaatgcac gttttttaga cgttcaataa taaaatcgtc tccccggccg
     6961 cgtttttgat cagcgctgat cataaataag accgcgtcca cttctttcaa cgcactgtaa
     7021 gctgcttcta ccataaaatc tcctaaacga tgctttggtt tatggattcc tggtgtgtca
     7081 ataaaaatca actgagcttc tggtgtagta tatactcctt ggattttatt tctagttgtc
     7141 tgcgccttat cactcatgat ggcaattttt tgacccacga ttcgattcaa taatgtggat
     7201 tttcctacat tcgggcggcc gacgattgcg acaaaccctg atttatgttc tggcatatac
     7261 gttctccttt ttaattaaac aacaaatgat atattttagg gacaaataaa atcaacccaa
     7321 cgatgacggc aaaacaagcg gtcactaaaa ctgctccagc tgccatatct ttgatttttt
     7381 tcccaatcgg atgaaaatga aaatccgtaa acatgtctac gacattttca aaaacagtat
     7441 tgatgatctc tactatccat acgagaaaaa cagctaaaag cagccataac cattctatcc
     7501 gatcaagttg gaaaatcacg cccataaaca aagcgaggat tccgaacgca acatgtttcc
     7561 tcatgttgcg ttcttcctca aaaacagttt tgactccttg gacggcaaat tccacggaca
     7621 tcaggaaatg tttgtttttc tctacgtttt tatcttttaa gtccataggc atctaagatc
     7681 tctttctgta aaccaaacat ttctttctca tcctctggtg tcatatggtc atacccgttg
     7741 atatgcaaga atccatgaac cgctaaaaag ccaagttccc gatcatacga atggccgtaa
     7801 gcttctgctt gctctgcagc acgttcagta gagatcatga tgtctcccag tacacgaggc
     7861 aatggattct ctccatcttc aaaaatcact gggatttcgt cttcaccctc ttcttccaag
     7921 gcaaaactga tgacgtctgt cggtgcatct tttccacggt aatcacgatt gattacttgg
     7981 atcgctgcgt tgtccatgaa agtaacagac atttctgttt cttctggcaa ctctaaatat
     8041 gatgctgcaa attgcaacaa gtcgtcgatt tctttcattt tctcttctga gacttggttt
     8101 gtttcatcca tgaaggtaat atccattata catcgcttct ttctattctt cttgctattc
     8161 ttcttgttct tcttttatct tctcaacttc cgatttcatt ttaggatatt tgatccgaga
     8221 atggaaggta ctgcttaaac cgctgatcaa tgatttttct actttacgga tttcttttaa
     8281 cgtcaatcca ctgtcatcca attgtccatc ggaaatacgc tcttctataa gattatggac
     8341 aaaactttcg attttttcgc ttgttggatg gtccattgcc cgtactgccg cttcacaact
     8401 gtcagctata ttgacgattc ctgcttcacg gctttgaggt tttggtccag gataacggaa
     8461 ttgctcttcc gtgacttccg ggttgcgttc ctttgcctta acgtagaaat aacgcatcag
     8521 cgtggttcca tgatgctgtc tgcaaatatc gatcaccatt tgcggcatat tataatcttc
     8581 caatattttg gcaccatctg tcacatgtcc aaagataata tgtttactgt cttctggcaa
     8641 taagaaattg tgcggatttt ccgctcctga aggcaagttc tcaacgaaaa agctggcgtg
     8701 tttgatcttg ccgatatcat gataatagca agctactcga gttagtaaag aacgtccacc
     8761 gatttctgct acagcattag cacttagatt ggctaccatc atgctatgat gatacgtacc
     8821 tggtgcttct tctaacaact gtttcaataa agggtgatta ggattgctga gttcatttaa
     8881 tacgatcaca ctatcatcat tgaccatcaa ttcaatatag gggtgcagac ctatcgtcag
     8941 caagaatgag aacaatgcac ctgtgaatcc gcagactagc gttaaccaag tcgttgcatc
     9001 cccaaagctc attccttggt agataatcaa aatgacatcc atcatcacag ggaaaataat
     9061 gacccacata gcagcgaata acccttgttc tgagatccgt ttgcgtctga cgatcgttgc
     9121 catgagacca gaaaatacat aggacatcaa aataactgtc agaaagttcg tgccaatcga
     9181 atcataaaaa atgaaaagtg cggatactgc ttggaaaata ccagccatga tccctgctct
     9241 tcgattcaag aaaaagctta aaacaagtgg agcaaaagcc gcggggtaaa acagcggaat
     9301 ataagcagct tgttctgttt ggaagagttg gaaaaatttc attagtacta cgctgactga
     9361 cattgctgtc acgtagaaaa gtacgtattt tgttctttct cctgcatttt gaaattgaag
     9421 tgaattataa atcagtacac cgatttgaag caaaacagct aaaaccatgg caatgagtgg
     9481 gaaaatcgaa gtcgtttgat tcgtcaatcc tagtaattcc aattttttca tcgctgaagc
     9541 atcaatttga tttccttcgc gaacaatgat ttctccttgg aagatcatca ctggttgtac
     9601 agcttctcgt gcagattgtt ttaattcttc tgtctttttc tcattcaata catcattgac
     9661 tacaatgcct tgatccacta agtagcggat catttgttgc tgctctgttg tgacattcaa
     9721 atactgaatc tgatcttctg cttcttgttt aaaagcttcc aattcgcttt cacgaacttg
     9781 tttactcatc tgttcgtcaa tgagcttcaa actttcatcc ctaaccgtgt gaatttccgt
     9841 ttcagtcatt tcaaatatcg tcgaataaaa attgttcggc agtttttggt aaaacgcaac
     9901 gttttctgca ttcacgtttt caaaattttt cttcagtgta gcaatccgct cttctgtagt
     9961 aggtttcggg atattttcat cttccttcgc cttatctacc ttgtcattat aagatttgtt
    10021 gatggtatca ttcgtcttat cgatcaattc gaataattgc ttgatccgat tgttctggtc
    10081 atctgccaga tctttttgat aagtatattc tggagtcact gcttctgctg ctagttttct
    10141 tctttgttcg gtttcttccg tgttctcgat cgttttattc gcacggatac tctcttctgc
    10201 tacttggcct tctctaaaat cattggcctt ttgtttgaca ctactaaatg taacgccgca
    10261 aacaatgata aaaaacaaga acagaaggaa aggcaatgct gcctttccga gtttttcttg
    10321 tagtttctgc agtgttttat tcaacatgtg cttatcctcc taagcaaagc ttattcttga
    10381 tgaagatccg ccttttcata agctttgatg atttcggcaa ctacagggtg ccgaacaacg
    10441 tcacttgctt caaaattaac aaaatcgatt tttttgattt gctttagtgt tcgttctgca
    10501 tgaacaagac cacttgtcgt ccctcgaggc aagtctatct gacttgtatc cccattgaca
    10561 atcatttttg attgataacc gagacgggtc aagaacattt tcatctgagc gactgtcgtg
    10621 ttttgtgctt cgtccaagat aacgaatgca tcatccaatg tccgcccccg catgtaagct
    10681 aatggtgcga tttcgattac gcctcgttcc atcaatcggt tcgtatgatc caatccaaaa
    10741 atttggtata aagcatcata aacaggccgc agataaggat ccactttttc tttcagatcc
    10801 cctggtagaa agcctaggtt ttcccctgcc tctacagcgg gccgcgtcaa aatgattttt
    10861 tgcacttcgc ctttttttaa ggcagcaatt gctaatacta cggcaagaaa cgttttccct
    10921 gttccggctg gccctacacc aaatacgatg tcatgtttgg ctaccgattc aacatatttc
    10981 ttttgaccgc tgttttttac ccgtataggt tttccattac ggtctttgac gatctcttcc
    11041 tcatacatct caacaaaata atctaatgta cctttttgcc ccattttcaa tgccgtcaca
    11101 acatcgggcg tacttacgtg gattcctcgt ttgatcaatt cctgtaatgt ccgaatgact
    11161 gaggcagcca aggaaatctg ggattgttca ccaatgatct gaatgatttc tccacgtgta
    11221 tggatggttg tttccgttgt ttcctcgatc agttttatat gcttgtcgtg tgcacctaaa
    11281 agcatgctga catcatcagc agctgtcaat ctaatctcta aagaactaga ctcttccgtc
    11341 aaaaatagac atcccctttt tttcgattca ttctttccta ttttaccata ctcagaagaa
    11401 actatatagc aaataagaaa aggttctaaa agagttgctt aaccataagt accaagaaac
    11461 gttaaaaaac tagccgcttc cattctctaa aggtctcgtt tgaataaaaa aagctgatca
    11521 tgtaagctat gcataagat
//
LOCUS       DACEPW010000078        11308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEPW010000078 DACEPW010000000
VERSION     DACEPW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.079"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..207
                     /locus_tag="IW819_001613"
     CDS             <1..207
                     /locus_tag="IW819_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244721.1"
                     /translation="NDRISFLTKKGEGLVLNLNIQHIDSIATFNQSVIQIKDHNMTEV
                     KNKIYAFSKKSNDKSYWETLAEII"
     gene            complement(298..1269)
                     /locus_tag="IW819_001614"
     CDS             complement(298..1269)
                     /locus_tag="IW819_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1244722.1"
                     /translation="MASVSGGRKPIYLSFNNECALVIAIEIGKDYIHSSLSYINGELV
                     NKLSYHSLLVTKDTLVMLLTKIISDLTSKIPQTPYGILGIGMAIHGIVKDDTVLFTPY
                     SDIDSIPLKDLLAKEIPYPIVLINEANAGALGEYTFKTPSENLVNINIQDGIGAGIVE
                     NGKLFIGANGTAGEIGHTTLYPDGILCPCGNHGCLEHYASTRILLKKLLDSDHKPHPD
                     ISVIVKKWHQGDAHTQKILKHNAKLLSIGINNIVLMYDPEVVIINSEIYRGIPELIDE
                     IKKHLTGKNTKNLAIKNTSLKGNSTLYGCVSIVAQSFLKINNLKFTV"
     gene            1701..3008
                     /gene="xylA"
                     /locus_tag="IW819_001615"
     CDS             1701..3008
                     /gene="xylA"
                     /locus_tag="IW819_001615"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAQ1244723.1"
                     /translation="MNYFEAVPKIQYEGKQTKNKFAFRYYNPEEEILGKPMKEHLRFA
                     VAYWHTITADGSDPFGAGTYQRNWEGNNPMETAKNRVEAFFEILEKLGAEYFCFHDVD
                     IAPQGDSLEEFFQNIDTITDLIKEKMDQTGIKLLWNTANMFTHPRFVNGAATTNNADV
                     YAIAAAQVKKGLDISKKLNGQNYVFWGGREGYESLLNTNMDFELDNLARFYKMAIAYT
                     KKIGHQPQFLIEPKPKEPSKHQYDFDAATSMAFIMKYGLQDHFKLNLEANHATLAGHT
                     FEHEINVARSYNALGSLDANQGDLLLGWDTDEFPTDIYSTTLAMYEVLENGGIAPGGI
                     NFDAKVRRTSFEMEDLILAHIVGMDTYARGLRAAAKMKEERFFEEIMEEKYASFTQGI
                     GKKIIDDQETFESLSEYALSLSDVTNQSSHIEYIKSLINDYLV"
     gene            3027..4520
                     /gene="xylB"
                     /locus_tag="IW819_001616"
     CDS             3027..4520
                     /gene="xylB"
                     /locus_tag="IW819_001616"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAQ1244724.1"
                     /translation="MGYVLGLDLGTGALKGVLVDETGVIRHIEACDYPLHSPKGGYNE
                     QNPADWLTACGALFEKFSCNVSDFQAELAGISFSGQMHSLVVTDEAGTVLRPAILWND
                     VRNSAQCKRIMGEFGNEIIAITKNRALEGFTLPKILWIQEHEPAIWEKVRKIFLPKDY
                     LRYYLTGHSHMDYSDAAGTLLMDTTKKEWSKPILEKYNIPEVILPPLVESSACVGTLK
                     PEIKATFGFEKEVRIFTGGADNAVSSLGSGLTDSHTALASIGTSGVFLALEEEDHTRY
                     GGKVHLFNHVLPDTYYAMGVTLAAGHSVSWFKNLLAPEQSYDEFLRNIPNVPIGSNGL
                     LFTPYISGERTPHVDSQIRGSFIGLSNHHTRDDLIRAVLEGVTFSLKDSQQLMTSLKG
                     EEFKRIISVGGGAKNKTWLQMQADIFNTEVVSLDTEEGPGLGAAILAAVGLGWFDSFE
                     SCTNQVIRYGTTVQPIPENVEKYNTVYDLYTKVYGATKDLSHALSIL"
     gene            4543..6600
                     /locus_tag="IW819_001617"
     CDS             4543..6600
                     /locus_tag="IW819_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HAQ1244725.1"
                     /translation="MITIEANRIVKKYDDELLIIEPWGQHSLRVRSFLDQHFVERENA
                     LTELHGYDEKLVETYYKNNQATIVNGKIKAVLDHRDRLTFYNEKDEVLLKEFIRLRAV
                     KHDDGSEDVGTVEFTKDFNSTLKLKSREYRANNDGSNFRTKVRFEPHAQEKIYGMGQY
                     QQNYLDLKNTMLELAQRNSQMSVPFYVSSRNYGFLWNHPGIGKVTFAKNMTEWEMYGT
                     DYIDYWITAGDSPKEITEQYTSVTGRVPEMPKKLLGLWQSKLRYRTPEEVLEVVEGYK
                     QRGIQLSTIAIDYFHWPKQGEYRFDPDFWPEPEKLVKTLKEDYNVEPLISIWPTVQSD
                     AYNYEQYLQGGYLIKVNRGVRLTMQIQGSTVFMDTTNPEARDYVWNLIKKNYKDIGID
                     YYWVDVAEPGYAVYDFENYRYHSGTAVESGNLYPNGYLKMVYDGNKDSNESVVTLVRG
                     AWAGAQKYGALAWSGDIDSSFEAFHNQVNSGLNMGMAGIPWWTTDIGGFHGGNPEDPE
                     FRELMVRWFQYSTFSPILRMHGDRLPHSKPLSNSGGGSMPTGAPNEIWSYGSEVENIM
                     TDYVEIRERMKDYLAESMTEAHELGYPIMRTLFYEFPEDKKSWEIDNTYMLGSDILVA
                     PIMNYKERGREVYLPSGEKWRHLFTGKEYAGGATYYVAAPLEQIPVFIKESASELFES
                     LGK"
     gene            6650..8707
                     /locus_tag="IW819_001618"
     CDS             6650..8707
                     /locus_tag="IW819_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 31"
                     /protein_id="HAQ1244726.1"
                     /translation="MLFKSKEVIVSEQSFVVKDRNQCLKVEGWGRDTVRVTCYPNHKG
                     FDPKQGIEELEKNVLTTATVTETSDQLILTNNRLTATYDGEKLVFYNNGTIILEEYSR
                     KQSQVRRTVGIDTHIPIPDEPTSSLNISPREFTYSGENTYQATLRFEGDPNEKLYGMG
                     GYQEENFNKNGGTYELMQRNSQTSIPFYLSDKQYGFIWNNAAIGEVTFGYNQKIWKSN
                     NTNLIDYLVTVGENPKQIIENYTQMTGRTPRLEPELLGLWQSKLRYQTLDELRTVYEG
                     YNQRGISLSVLVVDYYHWTSDGDFAFDSRYWEGIEDFAEEIKKTGTQLMVSLWPTVTE
                     ESRYYQSYRNNQLLIRSVTNKDSLFAGKALLDFFHPESKTFLRQVLDENYRSKGISLF
                     WADQAEPEMDVYQHKEYLIYEGSLEKYANKYPLHYLQAIQKNPERSNTEPTLIRSAWF
                     NAQKYGALAWSGDIESSFDSLRRQVQVGLSMGISGITWWTSDIGGFHSGNSQSPVFKE
                     LMIRWFQFAVFSPILRMHGDRQPHTQKIGNSGGGVRTSGGPNEIWSFGEEVEGILTRF
                     IHIRTRLKDYIRDVYNESATKGLPIMRSLFIEFPDDPKSWEETTHYLFGSDLLIAPVV
                     TEGATHIPVYLPKGCNWIQVFTKKVYPGGQIYKIAVTLNTIPVFCKEGAPLEHHLADI
                     FGN"
     gene            8724..9197
                     /locus_tag="IW819_001619"
     CDS             8724..9197
                     /locus_tag="IW819_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1244727.1"
                     /translation="MGLVLARIDERLVHGIVVTQWASAVKAKRIMVVDDQVAQDEPRK
                     AAMRLSKPAGTGMSLIDTETGITNFKAGKYDSHNVLLVVNNVKTLLDLSEQGIAIPKV
                     NIGIMLDRNDRKKLAKNFAASPEELQQLQQLADKGIPVSYQFAPSDKEEPLDKFI"
     gene            9222..10010
                     /locus_tag="IW819_001620"
     CDS             9222..10010
                     /locus_tag="IW819_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1244728.1"
                     /translation="MPEFLQNILIILIPMLTLIDNRGITIINHWPVTVGLLAGLVMGD
                     LQSAMVIAGTFQLMSLGVAALGGSSVPDYGLATVVAIYLNARTGAEIGTSIAVGLPVG
                     ILAINLDIFSRTINSFVSEKSKQYLEERKFSKMQWINFISVFFVAMQAFIPMVILVAF
                     GQVAIETATQWIPEWITNGLNVAGGMLPVVGVAMLMRYMPTKRYIWALLAGFIMAAYM
                     ELPILAVSIVGLALAVFIYNNLTSQKVAVTTENAAYDEGDDFDE"
     gene            10003..10833
                     /locus_tag="IW819_001621"
     CDS             10003..10833
                     /locus_tag="IW819_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1244729.1"
                     /translation="MNNERTKGLTQELNKKDRRNAAWRWSFIGSNNINYGTLQGTGYA
                     WSMAKTLRKIYPNDDDYVEAMRVEYEYFNITPYMGPLVAGADVALQEQQGTESLGAVR
                     SIKTSLMGPLSGIGDSLLWVLYPTIMGSISGYMALEGNPLGAIIWIILNLGLVWMRLK
                     LFDLGYESGIRLITDMADKLNAITESASVMGLTVVGSLIATVVKVYTPIEFTFGEVTM
                     GLQAEVFDRILPALLPVLLTALVYFLMEKKKWGFLKVIFLLLAFSLLGAYFGFLGVAP
                     "
     gene            10854..11273
                     /locus_tag="IW819_001622"
     CDS             10854..11273
                     /locus_tag="IW819_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HAQ1244730.1"
                     /translation="MKKIIVATHYRLAEGFKSTLGYIAPNMVEIIAINAYLENTSVEN
                     QVQAALDQFEAEEQILVFTDLLGGSVNQTFAKFLDRYNIQLIAGVNLPILMTLALAIG
                     SNDLSETTIRQAIEESRQQIVYVNDLLQVSAMDDMDE"
BASE COUNT         3496 a         2167 c         2538 g         3107 t
ORIGIN      
        1 aatgatcgga tttcattttt aacaaaaaaa ggggaaggat tagtgttaaa tttaaatatt
       61 cagcatattg acagtatagc tactttcaat cagagcgtta ttcaaataaa agatcataat
      121 atgacggaag taaaaaacaa aatttatgct ttttctaaaa aatcaaatga taaaagttat
      181 tgggaaacgt tagctgaaat catttagtga aaaaaatgct gctaagtact tacaccctaa
      241 taaaatcata tcctcccgaa aaataggaag ttttaaggag gatatttttc tatcacttta
      301 cactgtaaat ttcagattat tgattttcag aaaggactgg gcaactatgg aaacacagcc
      361 atagagtgta gaattacctt ttagagatgt attcttaata gctaagtttt ttgtgttttt
      421 accagtcaag tgcttcttaa tctcgtcaat caattcggga atgcctcggt aaatttcact
      481 gttgatgatg acaacctctg ggtcatacat tagcacaata ttgttaatac cgatgctgag
      541 taatttcgca ttatgcttca gtattttctg ggtatgtgca tccccctggt gccatttttt
      601 aacgattact gagatgtccg gatgtggttt atgatctgaa tcaagcagct tcttcaacag
      661 aatacgggta gaagcataat gctccaaaca cccatgattc ccgcacggac atagaatgcc
      721 atcggggtaa agggtagtgt gtccaatttc ccctgctgtg ccgtttgccc cgataaacag
      781 cttaccattc tcaactatac cagccccaat cccgtcttga atattgatgt taaccaaatt
      841 ctccgaaggg gttttaaacg tatactcccc aagagcaccc gcgtttgcct cattaattaa
      901 gacgattggg tagggaattt ctttggcaag taggtctttc aaaggaatgg aatcaatgtc
      961 tgaataaggc gtaaacaaga ctgtatcatc tttcacgatg ccatggattg ccatcccaat
     1021 tcctaaaata ccataagggg tctggggtat tttcgaagtt aaatcggaga ttatttttgt
     1081 caacaacatg acaagtgtat ccttggttac taagagggaa tggtaactca atttattgac
     1141 caactcccca ttgatgtatg acaaagaact atggatatag tccttcccaa tctctatcgc
     1201 aataacaagg gcgcattcgt tgttaaaact caagtaaata ggttttctac cgcctgatac
     1261 ggaggccatt ccagtgttta attcgtagac gagtgatttt tcaatcaatg tttttgtgat
     1321 atcggaaata gacgacttcg ttaagtttgt taatttcgct aattttgatc gagaaacctc
     1381 ggcgttttga atgatgaatt cgaggatggt cgactcattg taatgtgtcg gtgtgtaatc
     1441 attcaattta gtctgctcct ttaattaaga taatttcgaa atacttcaaa tatgaaatag
     1501 attttattaa atcatttact ataatttcaa tcttgtcaag gattctaaag gaagtcccag
     1561 ggaaaaaata taaatcgcac ctaaccaacc aaggtaattt ttaaaaaacg aaagcgcttg
     1621 acaagctgtt cccacccagg taatataatc atatcaggtt agtacgagtt tcgaactaac
     1681 taagaaaggt ggaattagca atgaattatt ttgaagcggt accaaaaata caatacgagg
     1741 gcaaacagac taaaaataaa tttgcttttc gctactataa tcccgaagaa gaaatactag
     1801 gcaagccgat gaaagagcat ctccgttttg cggtagcata ctggcacaca attacggcag
     1861 acggctccga tccattcggt gcaggtactt accaaagaaa ttgggaaggc aacaacccaa
     1921 tggaaacggc taaaaaccgt gtggaggctt tctttgaaat tttagaaaaa ttgggagcgg
     1981 aatatttctg tttccacgat gtcgatattg ctcctcaagg tgattccttg gaagagtttt
     2041 tccaaaacat cgataccatt accgatctga ttaaagaaaa aatggatcaa acgggcatta
     2101 aattactttg gaacactgct aatatgttta cacatcctcg ttttgtcaac ggagcagcaa
     2161 caactaataa tgctgatgtc tacgctattg ctgcagcaca agtaaaaaaa ggtttagata
     2221 ttagcaaaaa attaaatggt caaaattatg ttttttgggg tggaagagaa ggctatgaaa
     2281 gtttattgaa taccaacatg gattttgaat tggataactt ggctcgtttc tacaaaatgg
     2341 cgattgccta tacgaaaaaa attggccatc aaccccaatt tctgattgaa ccaaaaccaa
     2401 aagaaccatc aaaacaccaa tatgattttg atgcagctac ttcaatggcg ttcatcatga
     2461 aatatggttt acaagaccac tttaaactta accttgaagc caaccatgct acgttggcag
     2521 gacatacgtt cgaacacgaa atcaatgtag cccgcagtta caacgcattg ggttcgttgg
     2581 atgctaacca aggtgatttg ttgttggggt gggatacaga cgaattccct acagatattt
     2641 attccacgac tttggcaatg tatgaggtct tggaaaatgg ggggattgca ccaggtggta
     2701 tcaacttcga tgctaaagta cgtcggactt ccttcgaaat ggaagatttg attttagcac
     2761 atatcgtagg gatggatact tatgcacgtg gtttgcgtgc cgctgctaag atgaaagaag
     2821 aacgtttctt cgaagaaatt atggaagaaa aatatgcaag cttcacccaa ggaatcggta
     2881 aaaaaattat tgatgaccaa gaaacctttg aatcgcttag tgagtacgca ctttcccttt
     2941 cagatgtgac aaaccaatca agtcatatcg aatacatcaa atcactgatt aatgattatt
     3001 tagtatagaa agtcggggtg taaaacatgg gttatgtatt agggctagac ttaggaacag
     3061 gggcccttaa aggagtgttg gtggatgaaa ccggggtaat tcggcacatt gaagcttgtg
     3121 attaccccct tcatagccca aaagggggct ataatgaaca aaatcccgct gattggctca
     3181 cagcctgtgg tgcattgttc gaaaaatttt cttgcaatgt ctctgatttc caggctgaac
     3241 tagcaggaat tagtttttcc ggacaaatgc attccttggt cgttacagat gaagcgggaa
     3301 ctgtcttacg accggctatc ctatggaatg atgttcgaaa ttcggcccaa tgtaagcgca
     3361 ttatgggtga atttgggaat gaaatcattg caatcactaa gaacagggct ctggaaggat
     3421 tcaccttacc aaaaatcctt tggatacagg aacatgagcc cgctatctgg gaaaaggtac
     3481 gtaaaatatt tttacccaag gattacttac gctattacct gacgggccac agccacatgg
     3541 attattccga tgccgccggt actttattga tggatacgac taagaaagag tggtccaaac
     3601 ctatactgga aaaatataac attcccgaag ttatcttacc cccattagtg gaatcatccg
     3661 cttgtgttgg aacattgaaa ccagaaatca aagcgacctt tggctttgaa aaagaggtac
     3721 gaatttttac cggtggagcc gacaatgctg tgtcctcatt gggatctgga ttgacggatt
     3781 cccataccgc tttagcaagt atcggtactt ccggtgtgtt cttggccctg gaagaagagg
     3841 atcacacgcg ttatggtgga aaagtccatc tattcaacca tgtattgcca gatacctact
     3901 acgcgatggg cgtcacattg gctgctggac atagcgtctc atggttcaaa aatttactgg
     3961 caccggaaca atcatacgat gaattcttaa gaaacattcc taatgtgccg ataggcagta
     4021 atggactact atttaccccg tatatttctg gggaacgcac accacacgtt gacagtcaaa
     4081 tccgtgggtc cttcatcgga ttgagtaatc accatacccg ggatgacctg attcgggctg
     4141 ttttggaagg ggtaacattt tccttgaagg attctcaaca acttatgact tctttaaaag
     4201 gcgaagagtt caagcgcatc attagtgtgg gtggcggcgc taaaaacaag acttggttac
     4261 agatgcaggc tgatattttt aacacggaag tcgtgagcct ggatacggaa gaaggtccgg
     4321 gtttgggagc agcaatcttg gctgcagtcg gtctaggttg gttcgattct ttcgaatctt
     4381 gtaccaacca ggtcattcgg tatggtacta ctgtccaacc aattccggaa aacgtagaaa
     4441 aatataatac tgtttatgac ttatatacga aggtgtacgg tgcgacaaaa gatttgtctc
     4501 atgcgcttag tatattgtaa gtaattctaa taggaggaaa tcatgattac aattgaagca
     4561 aatcgaatcg taaaaaaata cgatgatgaa ttactaatta ttgaaccatg gggccagcat
     4621 tcacttcgag tccggtcgtt tttggatcaa cactttgttg agcgggaaaa tgcattaact
     4681 gaactgcatg ggtatgatga aaagctggtt gagacatact acaaaaacaa tcaagctacg
     4741 attgtaaacg gtaaaataaa agctgtcctt gatcatcgag atcgtttgac gttttacaat
     4801 gaaaaggatg aagttctctt aaaagaattc attcgtctga gggccgttaa gcatgacgat
     4861 ggcagtgagg atgttgggac agtcgaattt accaaagatt ttaattccac tttaaagttg
     4921 aagtctcgtg aataccgtgc aaataacgac gggtcgaact ttcgaacaaa agtccgattt
     4981 gaaccgcatg cccaggaaaa aatatatggg atggggcaat accaacaaaa ttatcttgat
     5041 ttaaagaaca caatgctcga attggctcaa cgtaattcac aaatgtcggt tcccttttac
     5101 gtttcaagcc ggaactacgg tttcttgtgg aatcatcctg gtataggaaa agtaaccttt
     5161 gcaaagaaca tgaccgaatg ggaaatgtac ggaacggatt atatcgatta ttggattaca
     5221 gccggagatt cacctaaaga aattacggag caatacacga gcgttacggg acgtgttcct
     5281 gaaatgccga aaaaactgct gggattatgg caaagtaaac tacgctatcg cactcctgaa
     5341 gaagtcttag aagtggtaga aggatacaaa cagcgaggca ttcagctatc gactattgct
     5401 attgactatt tccactggcc gaagcaaggg gagtatcgat ttgatcccga tttttggcca
     5461 gaaccagaaa aactggtcaa aacactcaag gaagattaca atgtcgaacc attgatttcg
     5521 atttggccaa ctgtccaatc agatgcctat aactatgagc aatatctcca aggtggctat
     5581 ttgattaaag tgaatcgcgg agttcgcttg acgatgcaaa tccaaggaag cactgttttc
     5641 atggatacga ctaatcctga agcccgtgat tatgtctgga acctaatcaa gaaaaattat
     5701 aaagacatcg gtattgatta ctactgggtg gatgtggctg aaccaggtta tgctgtttat
     5761 gatttcgaaa attatcgcta ccattccgga acggcggtgg aaagtggcaa cttgtatcca
     5821 aacggatact tgaagatggt ttacgatggc aataaggatt caaatgaatc cgttgtcaca
     5881 ttggtacgag gtgcttgggc aggtgctcag aagtatggtg ctttggcttg gtctggggat
     5941 attgattcca gtttcgaagc ctttcataat caagtcaact ccggattgaa catggggatg
     6001 gccggtattc catggtggac gactgacatc gggggcttcc atggtggtaa tccggaagat
     6061 ccggaattcc gcgaattgat ggttcgctgg ttccagtaca gcacgttttc tcctattttg
     6121 cgcatgcatg gagaccgtct gccacatagt aaaccgctct ccaattctgg tggtggttcg
     6181 atgccaacag gtgcaccaaa cgaaatttgg tcttacggca gtgaagttga aaacatcatg
     6241 acggactacg tggaaattag agaacggatg aaagactact tggcagaatc aatgacagaa
     6301 gcacatgaac tcggataccc aatcatgcgg acattattct acgaattccc agaggacaaa
     6361 aaatcctggg aaatcgataa tacctacatg cttggttctg acatcctggt tgctccgatt
     6421 atgaactaca aagaaagagg tagggaggtt tacttaccaa gcggtgaaaa atggcgacac
     6481 ttattcaccg gtaaagaata tgctggggga gcgacttatt atgtcgcagc acctcttgaa
     6541 caaatcccgg tatttattaa agaatccgcc tctgaacttt ttgaatcgtt aggcaaatag
     6601 cccataaaga gaggagataa aggacaagtc aattgggagg aaagaagcta tgctctttaa
     6661 atcaaaagaa gtgattgttt ccgaacagtc cttcgtcgtg aaggaccgta atcaatgcct
     6721 gaaagtggaa ggatggggac gcgacacggt gcgcgttacc tgttatccca accacaaagg
     6781 gttcgaccca aaacaaggga tagaagaatt ggagaagaac gtcttgacta cagcaaccgt
     6841 tacagagacg tcggaccaat taatcttgac caacaaccgt ttaacagcca catacgatgg
     6901 agaaaagttg gtcttttaca acaacgggac aataatcctg gaggaatact ctcggaagca
     6961 aagtcaagtc cgacgtactg taggcattga cacccacatt ccgattccgg acgaaccgac
     7021 ctcatcgtta aatatttcac ctcgagaatt cacctattct ggcgagaaca cctaccaagc
     7081 aactcttcgt tttgaaggtg atccgaatga aaaattgtac gggatgggcg gatatcaaga
     7141 agaaaacttt aacaaaaacg ggggaacgta cgagttaatg caacggaact cccagacgtc
     7201 aataccattc tatctctccg ataagcagta tggtttcatc tggaataatg cggcaatcgg
     7261 ggaagtaaca tttggctaca accagaaaat atggaagagc aacaatacga atcttattga
     7321 ttacttggtg accgtcggag aaaatccgaa acaaatcata gaaaattata cgcaaatgac
     7381 tgggcgcacg cctcggttgg aaccggaatt actggggtta tggcaaagca agctgcgtta
     7441 tcagacgttg gatgaattaa gaacggttta tgaaggttac aatcaacgag gcatttctct
     7501 ttccgttttg gttgttgatt actatcattg gacttcagat ggtgatttcg catttgattc
     7561 tcgctattgg gaaggaatcg aggactttgc ggaagaaatc aagaagactg gcacccaact
     7621 gatggtatct ctatggccaa cggtaaccga ggagagtcgt tattatcaga gctaccggaa
     7681 taatcaactc ttgatcaggt cggtcacaaa taaagattcg ctatttgccg gaaaagctct
     7741 gttagatttc ttccatccag agagtaagac attcctccgt caggtcttgg acgaaaatta
     7801 ccggagcaag ggaatttcct tgttttgggc tgaccaagcg gaaccagaaa tggatgtgta
     7861 ccaacacaag gaatatctta tatacgaggg tagcttagaa aaatatgcca ataaataccc
     7921 tctccattat ctacaggcaa tccagaaaaa tccagagaga tcgaacacag aacctacctt
     7981 aatccgttct gcctggttta acgcccagaa atacggggca ttagcttggt cgggggatat
     8041 cgaaagttca ttcgattcac taagaagaca agttcaagtc ggtcttagta tgggcataag
     8101 tggcattact tggtggacga gcgatatcgg aggtttccat agtggaaaca gtcagtcccc
     8161 agttttcaaa gaactcatga ttcgctggtt tcagttcgct gttttcagtc cgattctacg
     8221 gatgcacggt gatcgccaac ctcataccca aaaaataggg aattcgggag ggggggtaag
     8281 gacaagcggt ggaccaaatg aaatatggtc tttcggggaa gaagttgaag gcatattgac
     8341 gcgctttatc catatcagaa cgcgtctcaa ggattacatt agggatgtat ataatgagag
     8401 cgctacaaaa ggtttaccaa ttatgcgtag tctgttcata gaatttccag atgatcccaa
     8461 atcttgggaa gaaaccactc attatttatt cggcagcgat ttgttgattg ctccggttgt
     8521 gacagaaggc gcaacacaca taccagtcta cttgccaaag ggttgtaatt ggattcaagt
     8581 tttcacgaaa aaagtttacc ctggtggtca gatatacaaa attgctgtaa cactgaatac
     8641 cattccggta ttttgtaaag agggagcacc actcgaacac catctagcag atatatttgg
     8701 aaattgaaaa ggagaaatga aaaatgggat tagtattagc tcgaattgat gaacgactgg
     8761 ttcacggaat tgttgtgaca caatgggcca gtgcggtaaa agcaaaacgt atcatggtag
     8821 tggacgatca agttgcccaa gatgaaccaa gaaaggcagc tatgcgtcta agcaaaccag
     8881 cagggacagg aatgtccttg attgacacag aaactggcat taccaatttt aaggccggga
     8941 aatatgacag tcataacgtg ttgttggtag ttaataatgt taaaacgtta ctcgacttga
     9001 gtgaacaagg catcgccatt ccgaaagtga acatcgggat tatgctggat agaaacgacc
     9061 gcaaaaagtt agctaagaat ttcgcggctt ccccagaaga attgcaacag ttgcaacagc
     9121 ttgcagataa gggcattccg gtatcatacc aatttgctcc atccgacaaa gaagaaccgt
     9181 tagacaaatt tatttaataa attgagattg gaggaattat aatgccagaa tttttacaga
     9241 atatcttaat tattttaatc cctatgctta ctcttattga taaccgggga attaccatta
     9301 ttaaccactg gccagtaacg gttggtcttt tagctggttt agtgatggga gatttgcaat
     9361 ccgccatggt tatcgctgga acgtttcaat taatgagcct tggagtagcc gcattgggtg
     9421 gatcatcggt accggattat ggtttggcaa ccgttgttgc gatttacttg aatgctcgta
     9481 caggagcaga aataggaact tccatcgcgg tcggtttacc agtcggtatt ttggctatta
     9541 acttggacat cttttcgcgg acaatcaact ctttcgtttc cgaaaaatct aaacaatatt
     9601 tggaggaacg gaaatttagt aagatgcaat ggattaactt catcagtgtc ttcttcgtcg
     9661 cgatgcaagc tttcattccg atggttatcc tcgtagcctt tggtcaagta gcaattgaaa
     9721 cagccaccca atggattcca gaatggatta caaatggttt aaacgtcgct gggggtatgc
     9781 tgccagtcgt cggggtagcc atgttaatgc gctatatgcc aacaaaacgc tacatttggg
     9841 ctttgttagc cggttttata atggcagcat atatggaatt accaatcttg gccgtttcaa
     9901 ttgttggatt agcactggca gtttttatct ataataactt aacgagccaa aaagtggctg
     9961 taacaactga aaatgctgca tacgatgagg gagatgactt cgatgaataa tgagagaaca
    10021 aaaggactta cacaggaact aaacaaaaaa gatcgtcgta atgcagcttg gcgctggtct
    10081 tttatcggca gtaacaacat taactacgga accttgcaag gaaccggcta tgcctggtcc
    10141 atggcgaaaa ccttgcgtaa aatttatcca aatgacgatg attacgtgga agcaatgagg
    10201 gttgaatatg agtacttcaa catcacccct tatatgggac ccttagtcgc tggtgctgac
    10261 gtagcgctac aagagcaaca agggacagaa tccttgggag ctgtccgttc aatcaaaaca
    10321 tccctgatgg gtcctttgtc cggtatcgga gattccttac tatgggtatt gtacccaact
    10381 atcatgggat ctatcagtgg ctacatggct ctggaaggta acccattagg cgctatcatt
    10441 tggattatat tgaacctcgg attagtctgg atgcgtctga aattgtttga tttggggtac
    10501 gaatcgggga tacgtttaat cactgacatg gccgataaac tcaacgccat cacagaatca
    10561 gcttccgtca tgggactgac ggtagtggga agcttaattg cgactgttgt taaagtttac
    10621 acgccaatag aatttacttt cggtgaagta accatgggct tgcaggcgga agtctttgac
    10681 cgtatcttac cagcattgtt accggtcttg ttgaccgcat tggtttactt cttgatggaa
    10741 aagaaaaaat ggggcttttt aaaggttatc ttcttgctgc tcgcgttctc tttacttggg
    10801 gcctacttcg gtttcttagg agtcgcacct taatcatacg aggagggaaa taggtgaaaa
    10861 aaattattgt agctacacac taccgcttag cggaagggtt caagagtacg ttaggctata
    10921 ttgcaccaaa tatggtggaa atcattgcta ttaatgcgta cctagaaaac acttctgttg
    10981 aaaaccaagt ccaagcggca ttagaccaat tcgaagccga agaacaaata ttagttttca
    11041 cggatttgtt gggaggttcc gttaaccaga cttttgccaa atttttagac cgttacaata
    11101 tccaattgat tgcgggcgtt aatttgccaa tcttaatgac tttagcctta gcaatcggta
    11161 gcaacgattt gagtgaaaca accattcgcc aagccatcga agagtcccga caacaaattg
    11221 tttatgttaa cgatttgttg caagtatcag ctatggatga tatggatgaa taaaaaagca
    11281 aaaagattga attcgttcaa tctttttg
//
LOCUS       DACEPW010000079        11288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEPW010000079 DACEPW010000000
VERSION     DACEPW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.080"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW819_001623"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW819_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1244731.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW819_001624"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW819_001624"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1244732.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IW819_001625"
     CDS             complement(2169..2906)
                     /locus_tag="IW819_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1244733.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IW819_001626"
     CDS             complement(2997..3524)
                     /locus_tag="IW819_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1244734.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IW819_001627"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IW819_001627"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1244735.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IW819_001628"
     CDS             complement(4401..4886)
                     /locus_tag="IW819_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1244736.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IW819_001629"
     CDS             complement(5039..5236)
                     /locus_tag="IW819_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244737.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW819_001630"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW819_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1244738.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW819_001631"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW819_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1244739.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IW819_001632"
     CDS             9211..9423
                     /locus_tag="IW819_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1244740.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IW819_001633"
     CDS             9425..11104
                     /locus_tag="IW819_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1244741.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3274 a         2437 c         1980 g         3597 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcagg
//
LOCUS       DACEPW010000080        11237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEPW010000080 DACEPW010000000
VERSION     DACEPW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.081"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..1129
                     /gene="gmd"
                     /locus_tag="IW819_001634"
     CDS             83..1129
                     /gene="gmd"
                     /locus_tag="IW819_001634"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAQ1244742.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1209..2222
                     /locus_tag="IW819_001635"
     CDS             1209..2222
                     /locus_tag="IW819_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244743.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            2259..3203
                     /locus_tag="IW819_001636"
     CDS             2259..3203
                     /locus_tag="IW819_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAQ1244744.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWKNGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3271..4584
                     /locus_tag="IW819_001637"
     CDS             3271..4584
                     /locus_tag="IW819_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1244745.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVISASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4668..5570
                     /locus_tag="IW819_001638"
     CDS             4668..5570
                     /locus_tag="IW819_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAQ1244746.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSVSFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            5575..7227
                     /locus_tag="IW819_001639"
     CDS             5575..7227
                     /locus_tag="IW819_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1244747.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEKVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKADSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7251..8117
                     /locus_tag="IW819_001640"
     CDS             7251..8117
                     /locus_tag="IW819_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1244748.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8159..8350)
                     /locus_tag="IW819_001641"
     CDS             complement(8159..8350)
                     /locus_tag="IW819_001641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244749.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCGKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8380..8919
                     /locus_tag="IW819_001642"
     CDS             8380..8919
                     /locus_tag="IW819_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244750.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGADVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9047..9400
                     /locus_tag="IW819_001643"
     CDS             9047..9400
                     /locus_tag="IW819_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244751.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            9867..10343
                     /locus_tag="IW819_001644"
                     /pseudo
     CDS             9867..10343
                     /locus_tag="IW819_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10343..10537
                     /locus_tag="IW819_001645"
     CDS             10343..10537
                     /locus_tag="IW819_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244752.1"
                     /translation="MAETRPKGYPKLKEYTPNRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            10543..10828
                     /locus_tag="IW819_001646"
                     /pseudo
     CDS             10543..10828
                     /locus_tag="IW819_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            10842..11208
                     /locus_tag="IW819_001647"
                     /pseudo
     CDS             10842..11208
                     /locus_tag="IW819_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
BASE COUNT         3153 a         2303 c         2854 g         2927 t
ORIGIN      
        1 ttatcgtgta ttcttgaaaa gtattagaaa ttacgagcca tttatatggt cttatcactt
       61 acataggaaa ggataatgta tcatgaaaaa agcgctgatt actggtatta ctggtcagga
      121 cggttcctat ctggcagatt tcctgctaga gaagggctac gaagttcacg gtatcactcg
      181 ccgtgcttct atttccaata ccgctcgcat tgaccatttg atgggcaaga tcacccttca
      241 tgacggtgac ctgtccgact catcttctct gattcgtatc atcagcattg ttcagcctga
      301 tgaaatctat aacctcgctg cacagagcca tgttcaggtt tcttttgatg ttcctgagta
      361 ctccggcgat gttgatgctt tgggcgttat gcgtattctg gaagcttgcc gtattctggg
      421 cctgacgaag aagactaaga tttatcaggc atctacttct gagctgtatg gcaaggttga
      481 agaagttcct cagcgtgaaa ccactccgtt tcatccttac agcccttatg ctgttgcaaa
      541 gcagtatggc ttctggatca ctaaggaata tcgtgaagct tatggcatgt tcgctgtcaa
      601 cggtattctg ttcaaccatg aatccgaacg tcgcggtgag aatttcgtca cccgtaagat
      661 caccctcgct gccggccgca ttgcagaagg cctgcaggac catctggaac tgggcaacat
      721 ggactccctg cgtgactggg gctatgcaaa ggactatgtt gagtgcatgt ggatgattct
      781 ccagcatgag actcccgagg acttcgtgat cgctaccggc gagcagcaca ctgttcgtga
      841 cttcaccgag aaggcattcg cagcaaatgg catgaccatt cgttgggaag gcactggcat
      901 tgatgaaaag ggctacgatg ttgaaaccgg caagatgctc gtttgtgtaa atccacagtg
      961 gttccgtccg actgacgttg acaacctgtg gggcgacccg actaaggcaa agaccgttct
     1021 cggttggaat ccgcagaaga cctcttacga ggaactggtt aacatcatgg caaagcatga
     1081 ccgtgaattg gcaaagcgcg aagctgcaat gaagaacgca gtcaagtaag ctgacattga
     1141 ataaacatat ctgtttctat ctttccagtc acggcttcgg acatatcgcg cggaatctgc
     1201 ccattattat ggcaacactc tgccgatgcg aagcgaagat tacactggtt tgcggcgcaa
     1261 atcatctgga ttttgcaaag cgcaatttgg agcaggaact gcccaaagag ctgatagacc
     1321 gcgtgcgcta tcgtccaata aacacagata tcggtctgat taaccgtgag ggcaccctac
     1381 tggtggacac gggtaagctg accgagatct gtgaatattt tttggcaaag gttccttctt
     1441 tggcaaagac agaagccgaa tggctcaagc gagaagctgt cagcgcagtg ctttgcgata
     1501 tgccaatctg ggcaatggat gcctgcgata tggcaggagt acctttcctg tatgtaggaa
     1561 acttcacttg gacagagctg tatcgcgaat ttttacccga acatatttgg aaagaatatg
     1621 ccaagcgtta cggtaaaatt cagcacacca tgctttatgc gctaaataac gaagaaatgt
     1681 tggaatttct ccaaaacagc gaaaaaacac cgacttccgt tgtggcgcgt ccgattcata
     1741 acgacgaagt gcaacgcatt aaatcacagc atgaaaaacc gcctgtattt gtcgcactcg
     1801 gtatgtccgc gcggttcaaa cagccaattg atgtcagtca cgcgccgtat gatttcatta
     1861 caacgctagg tgttccgctt gtgggagaaa acgtaaccgt tatttctaac agcaccccca
     1921 atacacagga ctatatagcg gcggcagatt acgttattac aaaggctgga tggggaactg
     1981 ttgcggagtg tattctcgca cagcgtccga tggcactgtt cgcccgcgat acagttttgg
     2041 aagatagaac aactatttgt aaattgcaaa aactgggtct ggcaatttcc gtcacaccgg
     2101 aaaacctcca ccgcgtagag cgggtgattg aggacctcaa acagctagac acctcaaatt
     2161 ttgatacatt ttatgatgct gcgagcgaaa tcgcagacaa attgatttct ctcatgcaat
     2221 gagagatgct ctaaatttag aaaggagcaa gcattattat gatggaaaaa gacgcaaaaa
     2281 tctacgtagc cggtcatcgt ggcatggctg gttccgcaat cgtgcgcgag ctgaaccgtc
     2341 aggactacaa caatattatc acccgcaccc ataaggagct tgacctgtgc cgtcaggacg
     2401 ctgttgaagc atttttcgca caggaaaagc cggattatgt atttctcgct gctgcaaagg
     2461 ttggcggtat tatcgcaaac cagaacgctc tggcagactt catgtatgaa aacatgattc
     2521 tggaaatgaa cgtcatcaac tccgcatgga agaatggctg taagaagtta cagttcctcg
     2581 gttcttcctg tatctatccg cgtatggcac cgcagccgat gccggaaagc tgtctgctga
     2641 caagtgagct ggaaaagacc aacgaggctt atgcactggc aaagatttct ggcctgaagt
     2701 actgtgagtt tctaaacaag cagtacggta ctgactatat ctctgttatg cctactaacc
     2761 tgtatggacc taacgacaac tatcatccta ctcacagtca tgtgctcccc gctttgattc
     2821 gtcgcttcca tgaggctaag gaagcaggtc tgcctactgt tacctgctgg ggtgatggtt
     2881 ctcctctgcg cgaattccta tatgttgacg atctggcaaa cctgtgtgtg ttcctgatga
     2941 ataactatag cggtgacgag actgttaatg caggtactgg caaggaactg tctatcaagg
     3001 aactgaccga gatggtagca aaggttattg gttacgaagg cgagattctg tgggatacca
     3061 gcaagccgaa cggtacacct cgtaagctgc tggatgtttc caaggcaacc aagctgggat
     3121 ggacatataa gactgagttg gaagatggca tccgtctgtc ctacgaggac ttcctgaata
     3181 acccgatgcg tgctgaacgc taatttaccg aggttgctgt gatttttgag gattgcagca
     3241 gcctctttct tataaaaagg agagatcatt atgaatattg ttttactgtc tggcggttct
     3301 ggtaagcgcc tttggccgct gtctaatgac atccgttcca agcagttcat taagattttc
     3361 aaaactgaag atggatatga atccatggtt cagcgtgtct accgccagat taagaaggta
     3421 gataacgacg caactgttac gattgcaacc tctaagacac aggtttctgc tatccataac
     3481 caacttggtg aggatgtggg tgtatctgta gagccttgtc gccgcgatac cttcccggca
     3541 atcgcattgg ctaccgctta tctggtagat gtgatgggca ttgatccaga agagtccgta
     3601 gttgtttgcc cggttgaccc ttacgtagaa gatgactatt ttgaggccct gaaatctctg
     3661 tccgatcagg ctgacaaagg cgaagctaat ctggtgctca tgggtattga accgacttat
     3721 ccttccgaga agtacggcta catcattcct gagagcgcag agcagactgc tttcgtaaat
     3781 accttcaagg aaaaaccttc tgaggctgtg gcaaaggaat atatcgcaca gggtgcactg
     3841 tggaacggtg gtgtgtttgt ctacaagctg aagtatgtca tggagcgtgc acatcagctg
     3901 attgactttg tagattatca ggatctgttc aacaaatacg atactctgac taagatttcc
     3961 ttcgactatg ccgttgttga gcatgaggac aagattcagg tacagcgttt tgctggtatg
     4021 tggaaggatc tggggacttg gaatactctg accgaaggta tggaggaatc cattatcggt
     4081 aaaggtgtta tgaatgagaa ttgcgatggc gtacacatcc tgaacgaact ggatgttcct
     4141 gtgttggcta tgggtctgca tgatgtggtc atctctgcat ctcgggaagg tattctggtt
     4201 tccgataagg aacagtccag ctatattaag cctttcgttg acaagttcga gcagcagatc
     4261 atgttcgcag agaagtcttg gggtagttat aaggttatgg atgtagaagc agagagcatg
     4321 accattaagg ttactctgaa tgctagtcac tccatgaatt atcacagcca caagaaccgt
     4381 gacgaggtgt gggtggttct ggccggtact ggtaagacca tcgtggatgg atatgagcag
     4441 gttgttaagg ctggcgatgt gatcaccatg agtgctggct gtcggcatac cattatggca
     4501 gatactgagc tgaagctgat tgaagtacag cttggcaagg atatcaatgt taaagataaa
     4561 cagaaatttg agttagagta ttaaaaaacg gaggagggct cgttatacag aaaccgtttt
     4621 tattgaagcc ttccgcaaag gattatctgt gggggggaac cgcctgaatg atgaatttgc
     4681 aaaaggaatt gagatgtctc ctcttgcgga aacttggaat gctctacgca cccggatgga
     4741 ccgagctttg ttgtgtctgg tcagagtatc aggggatgac cttgacggag gtgttgcaag
     4801 aacacccaga gtttctggga acgcatccgc agaccaaagg agaattgccg atcctgatca
     4861 aattcattga tgcgaaaaag gatttgtctg ttcaggtaca tcccacagat gaatatgcaa
     4921 ttgaaaaaga aaacgggcag cttggaaaga cagagatgtg gtatgtactg gatgccagct
     4981 taatctatgg gctgcagaaa ataacagaag aagctgtgat ccgtgaagcg attgctcagg
     5041 gaactgttga gcgttatctt cagaaggttc cggtcaaaaa gaatgatgtg ttctttattg
     5101 aggcgggaac gattcatgcg attggtgcgg gtgcactaat tgcagaaatt caggaaaatt
     5161 ccaatctgac ttatcgtctc tacgattatg accgtgttga caaaaatggt aaaaagagag
     5221 aacttcatgt agacaaagcg ctagaagtag cgaacttgaa agggttgtca gaaccgaaac
     5281 agccgcttcg tgttctaaaa tatcgtgctg gtgtagctgc ggaattgctt tgcagatgca
     5341 agcatttcga ggtctctcgt atgattgtca acactgagag aagacagacg gtacactatc
     5401 gtgcggattc agtcagcttc cgtgtgttgc tgtgtgtcaa tggctgtggt accgttcgat
     5461 ttgataatga atcgttggac atctataagg gggactgcct ttttgtgccg gccgattcta
     5521 ctgaaatcag cattcacggg tcgattcagt ttctggatgt gagaggataa taatatgaat
     5581 tataaagtag aatatgagcg ttggctgaat catgccaatg cagatattat agaagaactg
     5641 cgttcctatt ctgagaagga cattgaggac tctttctata aaaatttgtc tttcggtact
     5701 ggtggtctgc gtggtaccat cggtgcaggc actaatcgca tgaacgtaca caccgtaggt
     5761 aaggcaagcc aaggtctggc agactatctg aataagaact atgaagccgc ttccgtggtg
     5821 gttggctatg attcccgtat caaatctgat gtgtttgcaa agactgccgc agacatcttt
     5881 gctgcaaatg gtattcaggt gcacttgtgg ccagaactgc ttcctgttcc tacagtatct
     5941 tttgcgactc gttatctgaa ggcagatgcc ggtgttatga ttacggcatc tcataaccct
     6001 tctaaatata acggctacaa ggtatacggt gcagacggct gtcagattac taccgaggct
     6061 gctactgcaa ttctggcaga aatcgaaaag ctggacatct ttgctgatgt aaagatggat
     6121 ggcgatgctt ctaagattgc atccattcag tcggaagttc tgactgcttt catcgaagaa
     6181 gtgaaggggc agtccgtcct gtttggcgaa aaagtggaca agaatgttgc tatcgtgtat
     6241 tctcctctga atggtaccgg tctggtgcct gttacccgca ccctggcaga gatgggatac
     6301 agcaatgtga ccgttgtgga agaacagcgt ctgccggacg gtaatttccc gacttgtcct
     6361 tatccgaacc ctgaaattaa ggaagctatg tctctgggta tggagtatgc caagaagtac
     6421 aatgcggatc tgctgttggc cactgaccct gactgcgacc gtgttggtat cgcagttaag
     6481 agcgcacatg gtgactatgt tctgttgtct ggtaatgaga ctggtctgct acttctggac
     6541 tacatctgtt ctcagagaat taagcatgga aaaatgcctg ctgaccctgt gatggtcaag
     6601 accatcgtca ccatggattt gggtgaacgt atcgcatcca actatggtgt tcgtaccatc
     6661 gatgtgctga ccggctttaa gtttatcggt gagcagattg gtatgttgga aaaggctggt
     6721 aaggctgaca gctatatctt cggctttgag gaatcttacg gctatctgtc cggtagctat
     6781 gtccgtgaca aggacggtgt taacgctgct tatctaatct gtgaaatgtt ctcttactac
     6841 aagactcagg gtatcagcct gctggataag ctgaatgaga tttatacaaa gtatggctat
     6901 tgtttgaaca ccctgcattc cttcgagttt gacggtgcgg caggttttac caagatgcag
     6961 gaaattatgg ttgaattcca caagggtctc gatataattg gtggtaagaa ggttattgaa
     7021 actttagact acagtaaggg cctgaatggt ctgccgaagt ctgatgtttt gaagtttatg
     7081 ctggaagata actgctctgt tgtggttcgt ccttctggta ctgagccgaa gctgaagacc
     7141 tatatcagcg tcagcgcaga gaacaaggaa gccgccgaag ctatggaagc tattatggct
     7201 gctgaactgg cggaaatgtt cgagtaagaa aagaaaggag aaatcttgta atggaggaac
     7261 tgttttctaa cgctttgaaa tcggtgaagt caaatcgtgt gctgtacacg gcatcgttct
     7321 ttgcaagatc ctctcttttg tatttgcagg aaattggaca gctgactgct ctgaaggcac
     7381 atacatctag gcgtacagga ttgtcatctt acctttgttt cctggtagaa gttggttcag
     7441 gcacattgga gtataagggg cagaaatata cgcttcaagt aggagatatg gtgtttattg
     7501 actgtttgaa gccgtatgtc catgagtcat cagaagatga tttgtggact ttgcggtggt
     7561 gccattttaa tggcattggt atgacggaag tctacaaaaa ataccttgaa cgaggtggtc
     7621 aaccggtctt taaaacatca aaaacagata agtatgctga cttgctgagg agccttcata
     7681 tggtggcaga gggacaggag tatgtccggg atatgaaaat caatgttcta ctcagtgaac
     7741 tgctgatgca tttgatggaa gattcttgga atcctgacaa agtgccgaat ttctctacaa
     7801 aaaaaatgga attgcagaac ataaaacaat atctggatga ccattacacg gagaagatta
     7861 cgctggatca gttgagtgca gagtttttta tcaataaaca gtacttagct aagatttttc
     7921 gtgaaactta cggtacaact gttgtcggtt atctgaatca gaaacgaatc acacaggcca
     7981 agcacttgct acgctttaca aatctaagta tggaagagat tgcaacaaaa attggcataa
     8041 cagatgcaaa ctatttttcc agaacttttt tcaaggtgga aggtgttcgt cccagcgaat
     8101 tccgcaagat gtggtaataa aaagccggcg gtgttgagcc gccggtcagt acctgtcgtt
     8161 aacccgcatt caaagcttta atctcagctt tgatttcagc acgaacacga tagtaatctt
     8221 cagtttcgat ttcggtatcc agccgagttg ctgtctttcc gcagtcagga caacgctctg
     8281 gctgttcgtc ggcagggaag ctgtagtggc aggaatcaca ggtgtaaatg tgatcgtcac
     8341 gtataatcat cacctaccaa tccagtgctt agatttttca tgcaattact attatatcga
     8401 attggtgatt ccacaaaaat ccgcttcttg ataaatgtac gattcagagt gatatatagt
     8461 actgtactgc caaagggaat accacaacgg cagaaatgga tgattcacat ggaagaaatt
     8521 atgcagatga ttttagggaa cgcaaaattc cagctcggac aggtggtttc gacccctgct
     8581 gtattggaaa ctttcaaccc cctgcagacc gcaaacggca ttcccgaagc cttgagcaac
     8641 ctttggttta gacaggaaat tagtattgcg gagatcgagg acgcagttct ggatgccaag
     8701 aacggcaagg agctagcaaa cagcctcaac cgcatcaagc ttttccgcaa gttcaccctc
     8761 gaccgagagc tggacggcat ggtcagactg aagggcgcgg acgtctggaa gaacatcagc
     8821 tacctcaagg tctacaccgc cacgccgcct aagaaaccga cccccccaag gttccagtcg
     8881 aacggctgga gccttttctt ctctcataaa tcggcataat gtatacaaat acagctccaa
     8941 atgattgtgt agtatatgcc tgtggattga cttgatatag tcgagagact atggcaatat
     9001 acacacaacc aaaggggaaa atcccaaaaa caaggaggaa acgctaatgg caatgagcga
     9061 aaaggcaaag aaatatttga aggagatcaa gggtgcaaag accatcccgg agctgaagga
     9121 tgtggagatc gcaatcaagc gagacggtat ccttgcttgg gcagagttca ccaagttgaa
     9181 cgaggcggtg gaagagaaga aggtcgccct tcgcaagaaa aagcaggaga ccagccttca
     9241 ggagatcctt ttctgggcct acaaaaagga atcagacaag ctgctgaaga tgatggacga
     9301 gggagcagac aaggatgcca tccagatgca ggtccagcgt tacgacagca tcggccagat
     9361 cattgcagag gcagacctcg aagatgagta tgaggtttga gaatacagcc tgaaccacta
     9421 agaaccaaat accgaaaggg acttctgaaa cagacagagg tccctttctt ttgctcactt
     9481 tggcggtcaa ttatgaacca aaaaccagtc gaatgattgt gtacattatg gctttcaaac
     9541 gacttgataa taatcacttt tagagtgata tatgtactac aaaaaacaaa agactggaga
     9601 aaacaacaga tgagcgaata cagaatcatt tatatcagcg acgaggacgg cggcgaggtg
     9661 gaaggatgtg gctacacggc tacgatggtt atgatgcggt cagaaacttc ttggaatttg
     9721 cctgcggcat cttgaaaatc ctgcgggtgc gattcatcag aagagtggac tgaaatacaa
     9781 gttagcagat agagaagaga gtcaggtttc gacttggctc tgttctctgt ctactggcag
     9841 aaatacattg aagggaggtg acgactgtgg caaccagagg aagaaaatca accccgacag
     9901 ccatgaagaa gctggaggga aatccgggca atcgcccact caatgcaaaa gagtcaacgc
     9961 cggaaaagaa ggcaccgacc tgtccgaagt ggctggaacc agaggcaaag aaggaatgga
    10021 agcgtctggc aaagcagatg gaggccatcg gaatcttgac agatgtggat atggctgcct
    10081 ttgccggtta gtgccaagcc tacgcccgct ggaaggaagc cgaggaattc attacccagc
    10141 acggtatcat cgtgaaaacc ctatccggct attggcagca ggtaccacag gtgtttattg
    10201 cccagaccta tctgaaaatt atgaaccgat tttccgagca gttcggtctg acgccttcgt
    10261 ccagaagccg tatcattgcc ggtgatggcg gtggtgtcgg agcagaagat gcgatggagg
    10321 ctctgttggg aggtggtagc tgatggcaga aaccagacca aagggctatc ccaaattaaa
    10381 ggaatacaca ccaaaccgat tcatgctttc ggaatgtcac tacgacaaag ccagagcgga
    10441 tcgggcggtg aactttatcg gacagctccg acacaccaaa ggcaaatggg caggcaaccg
    10501 cttctggctc ttgccttggc aggaacagat catctagtgt aatctcgctt aactgtatta
    10561 acggacgtag cgactttatt aagagtgttc atggtaggag cattagttgc aacaatgatt
    10621 gatattaaat ttttatggta ccaacgattg gcgatgtgac attgaatatg caaaactata
    10681 tcgatatgat attatctaaa atcttactcg ctgggatcgt cgaggtgttt attggttgtt
    10741 aggaaagaaa aatatgaccc aactcgagcg atttttatcg tattacttgt gtgtggcttt
    10801 atctgcattg tgtgtgattt cgaaataagg gagagccaaa aatgagtaga aaattgatat
    10861 tgattagtca cggaaaattt tgtgaggaat tgaaaaaaag tacaaaaatg attatgggac
    10921 ctcaagagaa tatctacacg ttacctttat taccagaaga tggcactgaa acatacagac
    10981 aaaagctctt actgtcatag attcgttaaa tgattttgtg gtactatgcg atcttttagg
    11041 cggtacacct actaatatca tttctaaact gataatggaa ggacaacaga ttaaacttta
    11101 tgtcggatta atcttcctat ggtcattgaa tttatcaact cgcaagtgat tggttcagag
    11161 cccgagtttg ttcgtgcagg atgtgaaagc attgtatctg tcaactaatt aattagtgga
    11221 atagtagaag atgaaga
//
LOCUS       DACEPW010000081        11231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEPW010000081 DACEPW010000000
VERSION     DACEPW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.082"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            135..455
                     /locus_tag="IW819_001648"
     CDS             135..455
                     /locus_tag="IW819_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244753.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            907..1107
                     /locus_tag="IW819_001649"
     CDS             907..1107
                     /locus_tag="IW819_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1244754.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1254..4043
                     /locus_tag="IW819_001650"
     CDS             1254..4043
                     /locus_tag="IW819_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1244755.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4090..4617
                     /locus_tag="IW819_001651"
     CDS             4090..4617
                     /locus_tag="IW819_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1244756.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4638..5828
                     /locus_tag="IW819_001652"
     CDS             4638..5828
                     /locus_tag="IW819_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1244757.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5868..7166
                     /gene="asnS"
                     /locus_tag="IW819_001653"
     CDS             5868..7166
                     /gene="asnS"
                     /locus_tag="IW819_001653"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1244758.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7961..8635
                     /locus_tag="IW819_001654"
     CDS             7961..8635
                     /locus_tag="IW819_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1244759.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8632..8973
                     /locus_tag="IW819_001655"
     CDS             8632..8973
                     /locus_tag="IW819_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244760.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9003..9362
                     /locus_tag="IW819_001656"
     CDS             9003..9362
                     /locus_tag="IW819_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1244761.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9556..9744
                     /locus_tag="IW819_001657"
                     /pseudo
     CDS             9556..9744
                     /locus_tag="IW819_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            9886..>11231
                     /locus_tag="IW819_001658"
     CDS             9886..>11231
                     /locus_tag="IW819_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1244762.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPL"
BASE COUNT         3741 a         1989 c         2404 g         3097 t
ORIGIN      
        1 gaggttgtga cgtggtcacg acctcttttt gacgattatg atacaaaaaa catcatttat
       61 gaacaaaatg tgaataacgg gcgttcttga gatatgattt taattgggaa aaacttatcg
      121 ataaggaggt ctttatgaaa aaaagactgt tatggtgttt gctgtttcta tccctaagtt
      181 taacaagtca gccagttttt gctgaagaaa cacagcggtc tcctattaca ggcacacgtc
      241 tgtacaaaaa tgtttatgta cagacaacag atcttgattc tgcgattcct gaaacagaag
      301 aacggtcttt gcagccaaga tttggttact tttggtactg tccgacctgt gattatgaaa
      361 gttgctggca tttcttacat tctacagctg cagcttttgc ttttactcat actcagaaat
      421 atgatggaca tcatcctgtt atttatgagg tatagatatg agcaaaaaag aaaaactaat
      481 ctcgttgatc gtattggttt gccttgttct tctggtcact gtcataggct atcagaaata
      541 ccatgagaac atcagaccta ctaaagaatc ttccccagcc tcgaccgttg aaacagaacc
      601 gataagtagt cagaaagaat aattttttta gtttaaagaa gaacatcaga gcaaaaaaac
      661 accaactgaa caatatgtcg tttattttaa aaaaagttcg ttttttcact ttatgtaatc
      721 gctgagaaaa ctttttttaa taaaaaaagt cgaaaaactc tagctttttt gtaaacggta
      781 tgatatacta taggagtagt ttttgttcgg taatctgtgg tagcttgttt caaataaaca
      841 tcttccagat atacaccatt caagtaattg caaaatatgt gttgatacac aaggaggatt
      901 tttattatgg aaacaggtac agtaaaatgg tttaactcag aaaaaggatt tggattcatc
      961 actgcagaaa acggaaacga tgtattcgtt catttctcag ctatccaagg cgacggattc
     1021 aaaactttag aagaaggcca agcagtaact ttcgatgttg aagaaggcca acgcggacct
     1081 caagcaacaa acgttactaa agcttaatta acctatacta aaaaataaac gcccagacat
     1141 ttgcgtgtct gggcgttttt gtctattttc tttaaaaagc gatgagagta tttttctgca
     1201 ctttgtagta taatttcaaa agaacattag aggcaggaga agaggaagat attttgaaac
     1261 caagaactta tgcagtcgtt gatatcgaaa cgactgggac gaatccaaaa gaagatcgga
     1321 tcatccaatt tggctgcgtc atgattgaat caggtcggat cacttcacgt ttttcgatcg
     1381 atattcatcc agggcggaag atttcaaaac aaatccagca tttaacaggg atcacgaatc
     1441 aacgcgtcca aaaagcgcct tatttcgaag atgtcgccca aacgatttac aatcttttgg
     1501 cagacaccat atttgtcgcg cataatattt attttgatta taattttttg aaccatgaac
     1561 tcatgcgatg cggacttcct agtttgaaga taccaggcat cgatacagta gaacttgcac
     1621 aaattttctt gccgacggaa ccaagctttc gcttagctga tttgtcagaa agccttggat
     1681 ttattcatga gaatcctcat caggcagata gtgatgccga agtgacaggt caacttcttt
     1741 tgctgatcga agaacggatg agaaaattgc caatcatcac gttagagcag attgcgcgat
     1801 taagtagaca cacaggtatg gatacgagtc gtttcattta tcatgtgatt gaagaaatga
     1861 aagaaaaacc agagccactt gattcctcat tagaaatcgt cgatgggcta gctttgcaga
     1921 aaaaagaagt ggaactattc acatccgtcc attacggcga gcggacatat ccaagaaaaa
     1981 aacaagcaaa agaaaagatg tttggtaaaa cactgatgta tcgaaaagaa cagaacagac
     2041 tgatgaatgc gatctacgac catttcacaa aagatgaatc aaaagatttg atgatcgaag
     2101 cagctacagg aatgggtaaa acaatcggtt atctgttgcc attgagttat ttggctacac
     2161 ctgaaaaacc cgctgtcatc agtaccgttt cgctagtttt gcagcagcag atcatagaaa
     2221 aagaaattcc attgctgaat caattgctag atcagcccat acaacctgta attatcaaaa
     2281 gttatcgcca ctatatcgac ctgcaacgat ttaaaggaac acttgtggaa ccaccagaac
     2341 aaaaacaata tgctttgtat caaatggcgg tcttagtttg gctgacgcaa acgaagaccg
     2401 gtgatttgga tgaattacat ttaacaaact tgaaccatgc attctttgca gatatcgcgc
     2461 acagaggtac tgggttttta gcaagaaatc agtcgtttta cgaacaagac tttgttcgtt
     2521 tcttgcaaaa gaaaatcagg caaagtaatt ttttgattgt caatcatgcg tttttagtac
     2581 aagaaacaca gcgaaaagaa ccattgcttc cagctagtcc atttttgttg atcgatgaag
     2641 tacatcattt gccggaaatc gctgaaaaag taaatgacaa acgattaagt attaactatt
     2701 tccataaaca aatccagcaa ttagaagaac caaatcagct gtttgaccga atacaaagct
     2761 atttaccgaa agaacatgaa gccttacgct ggttatcgat ttatcgagaa gaactttctg
     2821 cgctatgcga agcacagatt tgtatggcag aaggattaat gagtttagtg agcggcaagc
     2881 aacccaacgg acagtatcca gaagaagtca tgatgacaaa agagatgctg gatcagctgc
     2941 cactggaatc tgggacagcc attgatcacc tgcagctttt ttatcaggag attcagcagc
     3001 tacagtcaca aatgcgtctg acttttgaga ttatccaaga aagctggaca aaccagatgc
     3061 ggatcgattt agcaagttta tatagcttat ttgatcaatt agacaagcag tacttgctga
     3121 tggatcaatg gctggaagac tggcgagatc atttgatcca ttggttcgtt cctgggaaca
     3181 cagcgcagca gatgattttt catctccatg atttccaagc ggctatgttg ccgtcgactg
     3241 tttggtatcc tcgatatgat cgtattcttt atattggagg aacgctgaag atcggcccta
     3301 atcgacacta tctctctaag aggcttggca ttgaagcagc acctcttaaa gtgattgcct
     3361 ctccttatga ttatgagaaa caggcaagga tctatgtgcc aaaagaggca cctgccgtcc
     3421 atcaagtcag caatcaagca tacatcgatt atttagagac agcacttaca ggtctgatcg
     3481 aacaagaaga tcggccaata cttgtgttat tcacttctca tgatgttttg cagaaggtct
     3541 atcagaaaat gcatctgtct atgctagaaa aaggacgaga aatactagct caagggtttg
     3601 gtggaagccg ggagaagctt ttgaaacgct tcattttgtc agaaaaaagc gtattgtttg
     3661 gcgctgacag cttttgggaa ggtgtagatt tgcctggaga tgctttgcag atcgttgtag
     3721 tcacacgttt acccttcgat aatccgcttc gcccgatggt caaagctagg aacgcttatt
     3781 tagaatcaga ggggatcaat cctttttacc aggaatccgt acctaaagca gctttgaagt
     3841 tacgccaagc attaggtcgc ttgataagga cggagacaga tcgtggcgta ttgcttattt
     3901 tggatcgaag actagtcact gcaaaatatg gcgcacggat catccatgca ttaccgaaaa
     3961 aagtaccgat caaagagcta gctatggagg aaatctatca ggatatccat gaattcttaa
     4021 agaaaaatga agaaggaagt tagaggagct gtcttttttc ataattaaaa tctgctataa
     4081 tgaaaaggaa tgaagataaa ggagcgacga cccttgaaga aaaaacagac aaaggaaaaa
     4141 acgattaatc gcgtgttact tggcttgatc gtatttttat tagcggtcat cgtgatcagc
     4201 tcgatttttt acttgcgttc cagtcgtccg gcggtgcagg cgaagaaaga agcagtagaa
     4261 atcgcaaaga aatatgcaaa tcttgaggaa accgatcatt tttattggtt tactcgaaag
     4321 aagacatact tttcactgac aggtacaaat gataaaggaa aagaaatcgc cgttatcatt
     4381 cctaaatctg gtgaaaaagt aaaagtattg gatcaaacaa agggactgac tgaaaacgaa
     4441 gcaaaacaga caattgctgc ggcccatcct gaaatccaaa tcgaaaaagc tgctttaggg
     4501 atgtatgatg atcagcctgt gtgggaagta gtaggaaaag aaacaaatgg gaaactgaat
     4561 tattacctga ttggctttga atctggagat gaagtcaaga caatcaaggg aatttaaaag
     4621 gtaaaggaat gaatgggatg gagttatcaa aaagaacaaa acgattagaa ccgtctgtca
     4681 ctttggcagc tgcggcaaag gccaaggcat taaaagcaaa aggcagagat gtgctgagtt
     4741 tgactgtggg agaaccggat tttgcgacac cagaaaacat ccaagaagca gcaatcgaag
     4801 caatccgcaa tgggaaagct agctattata caccaacagc aggaatccca gagcttcgac
     4861 aggcaatcgt tgattatctg aagaaaaact atggacttgt ctatgaacct tcacaaacag
     4921 tcgtaacaga cggagcgaaa tttgctttgt ataccctttt ccaaacgatc cttgacccgc
     4981 aagatgaagt catcattcct gtgccttact gggtcagtta cggtgagcaa gtaaaactgg
     5041 cagaaggact tcctgtcttt gtgaaagggg aagaatccaa tagctggaaa gttactgttg
     5101 aacagctaga acaagcacgt tcagaaaaaa caaaagccgt aatcatcaat tctccatcga
     5161 atccgactgg aatgatttat agcaagaatg aacttcaggc aatcggtgaa tgggcagtaa
     5221 aaaatgatat tttgatagtt gctgatgata tttatggtcg tttagtttat aatggcaatg
     5281 agtttactcc aattgcaacg atttcagaag caatcaaaaa tcagaccatc atcatcaatg
     5341 gcgtgtctaa aacatatgcg atgacaggct ggcgcattgg ttatgcggtg gggaattctg
     5401 agatcatcaa tggaatgatt gctgttgctt cccagtcaac cagtaatccg acagcagtca
     5461 gtcaatatgc agctgttgaa gcattgaaag gtgagcaaga tacagtagaa gaaatgcgga
     5521 acgcctttga agaacgcctg aatacgttat atccgcttgt ggcagaactg ccaggtgttt
     5581 ctcttgaaaa gccgcaagga gctttctatc tttttcctaa tgtaaaagaa acgctacgca
     5641 tgtgtaaata tgaaaatgtc acacaatggg tagaagatct tttagaagaa acaggggtag
     5701 cgcttgtgac tggcgaaggc tttggggcac cagaaaatgt ccgcatgagt tatgctactg
     5761 atcgcgctac gttagaagaa gcggtacgca gaatcaagca atttattgag agtaaaagtc
     5821 aaaattaatc ctgaagaaat tcaaggaata taaaaggaga gtcatacgtg gaacaaattc
     5881 aaatcatcga ttcgaaaaac catgtgggag aaacagtaac aatcggcgct tgggtcgcaa
     5941 ataagcgatc aagcggaaaa atcacatttt tacaattaag agacggaaca gcctttttcc
     6001 aaggtgtagt tgtaaaaagc gaagtgtcgg aagaagtttt ccaaacagct aaaaatctac
     6061 accaagaaac atccgtgtta gtaactggag aaatccgtga agacagtcgt tcaaaatttg
     6121 gttatgaaat cggaattaca aatgtggaag tggtaagcga gagccacgat tatccgatca
     6181 caccaaaaga acacggaaca gacttcttga tggatcatcg tcatttatgg ctgcgttctt
     6241 caagacaaca tgcgattatg cagatcagaa atgaaatcat tcgtgcaacg tatgaattct
     6301 ttaatcaaca tcattttgtc aaaattgatc cgccgatctt gactggttct gcaccagaag
     6361 gcactacgga tctattcgaa acaaattact ttgatcaaaa agcttattta tctcaaagtg
     6421 gccaattata catggaagct gctgctatgg ctttcggaaa agtcttttca ttcggtccga
     6481 ctttccgtgc tgaaaaatca aaaacacgac gtcatttgat cgaattctgg atgatcgaac
     6541 cagaaatggc tttcactcat caagaagaaa gtttagaagt acaggaacaa tatgtagcat
     6601 tcttagtaca aaatgtttta gatcattgcg aatacgcatt agatgtacta ggacgagaca
     6661 aagaagtctt aaaaacttat acaaaattgc cattccctcg tattacttac gatgatgcaa
     6721 tcgaattact gaagaaaaat ggatttgatg acatcgaatg gggcgaagac tttggttcac
     6781 ctcatgaaac gttcattgcg aattcattcg aacagcctgt ctttatccta aactatccaa
     6841 aagccatcaa gccattctac atgaaaccac atccaacaag agaagacgtg gtaatttgtg
     6901 cagatatgat cgcaccagaa ggttacggtg aaattattgg cggaagcgaa cgggcaacgg
     6961 actatgattt cttgctagaa caaatccgta atcacggttt ggatgaaaaa gaatattcat
     7021 ggtatcttga tctacgtaaa tacggttctg ttccgcactc tggttttggt ctaggtcttg
     7081 aacgaacagt cacttggatt gctggtatcg accatgtacg tgaagctatt ccattcccac
     7141 gtttgttgaa ccgaatttat ccataagagg ttgtgcgcct gccgttcagc tccgagtcac
     7201 aaggagtaat ttcaaaaaac agtttttcat gtttgatgac tattgtgact tgtggccgga
     7261 gagcaacgtt tatttaaaaa aaagaactgc gaactctcgc agttcttttt tttaaatacg
     7321 ggataatgga agtttccagc agacaagaga agaaagcaaa agaaattgct atttgattca
     7381 caaggttcta taacgaaatc gcaatcaatc aaacatttac aaagaaggta attcaaatgg
     7441 aaatctgatt tgatgatgct tctgctgttg aaataaagca gcatctagca cctgaaaaca
     7501 aactaatagt aacattcgaa gatggagtag gtccgtattt tcagcatgca ataatccata
     7561 tgtaagttca attcaccctt gaaatcatcg gaccagataa agttatgacc aatctatccc
     7621 aagcaatcta gaagagatat tagtaaaggg acatttacta gaagtcttag atgagcctat
     7681 aaatatatgt ttcgatggcg tcatcagtgg tctgattgac ggaagcttgg gtgtgatcga
     7741 tctctctgga ccaaacaacg aaggaatcaa ggggaatccg gcacgttaag aaaaaaatag
     7801 cataaaaaac tagacacttg atgagcgttc attcaattgt ttgaaggaac taggctggtg
     7861 tcatttttta tacatatatt ttctctttcg tcttgatagg gcttatggga taatataagg
     7921 aagaaaatca tcgttttatt caaaaaggag tttttgacaa atgaaaaaac gtatattatt
     7981 tatcggtgat agcattacag acgcaggaag ataccgagca gaagcaactg atctaggtaa
     8041 aggttatcca cttttgattg ccggaaaact aatctcccaa tatccaacga tggaatttga
     8101 atgcatgaac cgtgggatag gaggagacaa aattgcagat ctgcaagtac gctggcaaga
     8161 agattgttta gctttacaac ctgactatgt atcgattttg atcggaatca atgatacctg
     8221 gcataatact agcgatcagg ctatattcgg aactgagaaa tctgctcagc aatttgaatc
     8281 cgtttatcga aaattactaa atgaacttca aacagcggaa atctgcaaca tcatcttgat
     8341 ggagccgttt gtacttccta taaccgacga ccgtcatggc tggcgtaaag acttagaccc
     8401 taaaatccaa attgttcgta aacttgctaa ggaatatggc gcattgctgg ttcctttaga
     8461 tggtatattc aatgctttag ggattgctta tggtttttcg aaatatacaa gagaagatgg
     8521 ggtccaccca acgattggcg gacatgagat cattgcacaa agctggatag atgcaattgt
     8581 cgcagtcgat ttttttagtg gagataaaaa tgagcgaaag aaaagtgaaa aatgatggaa
     8641 ataaggtggg cagtaaaaga agatttacct caaatcaatc agctgattac tgatcatcca
     8701 gaatcattcg taaaagcaaa aacggcagct gacctaaatg ataagaataa actattgctc
     8761 gttgctgtaa caaaagaaaa acggattatc ggatttgcgc aagcggacgt gcaacagtca
     8821 gaagaacgat tggatcagcg accatcagga aaactaatcc aacacacatt agtaacaaaa
     8881 gaaaatacgg aagaaatcca aaaaaaattt gcagaatact tgatgctgga agctaaaaaa
     8941 agaagaataa aatcaatttt tgcccagttt taatcaaaaa cagtaataga aggagaaata
     9001 caatgagttg tttaggaaat gtcatctggt ttatattcgg cggactaatc ggtggtctta
     9061 gctggttagc agcggggata ctttggtgta tcacgatcat aggtatccca atcggtatgc
     9121 aatgttttaa gatcgcctcc ctttcttttg cgccttttgg caaagaagta atttatcacc
     9181 agtctggcat gtcattgatc gttaatatca tctggctgat cgtttcaggc ctaccaattg
     9241 ctctcgcaca tgtcgtcagc ggcatccttc tatgtatcac gatcataggg ataccttttg
     9301 ccaagcaatc tttcaaactt gctcgattgg cgttgatgcc attcggtgca gaaattgtat
     9361 aaaagagaat aataaaggtt agataaagca tagagccagg tgggatacat gtatttcacc
     9421 tgactctatg cgttttcctg tataagttct tctatgtgac ttttacgtct tttatttata
     9481 tggggttact gtttatggaa agttgaaata attttcatta aagaactctt tctatatttt
     9541 tgaaagcgta gtggagcttc gtatatctgg gaaggacatt ttgcagggat tgaaaaaaga
     9601 tattgtccta tcgaaaaatt aatgaattta gacaaattgc cagcaacagg cttcaaactt
     9661 agttgctatc ctgtcaagat caagaaggca agtgcgggtt ggataagagc agtggcaatg
     9721 atcgaagaga aaaagaaaga ataagccaaa acatctgtat ggaagaaatt ttccgatgca
     9781 gatgtttttc attttttctt tcggatacct tgacatatga ataaccattc atttataatt
     9841 agttatgaag agatattcat atgttgaggc gaggaggtca ttaaaatgaa aagttacaga
     9901 ttagaaggtt tagattgtgc gaactgtgca atgaaaatcg aaaaaggagt tcagaaaatc
     9961 aatggagtca aggaagcaac ggtaaacttc acttctggaa aactaacgat tgacgcagag
    10021 gaagatcatt tggctacgat tgaacaagaa acaaaaaaag tagtgaagga attagaacca
    10081 gatgtcaaag taacagaaat agataaggaa aaagtatctg aacatggaaa tgaaaaaaag
    10141 agaaatacgc tgttccggat tttattttcc cttgcaggta tcgctttatt gctattattt
    10201 gattttaatg aaccaattcg cttgataggg tatttattga tttatctgct gataggatat
    10261 gatgtagtga agaaggcagt gatgaatatc gtcaaaggaa aaatctttga tgagaatttt
    10321 cttatgtctg ttgctacgat tggcgctatg ttgatcggtg aatatccaga agccgtagca
    10381 gtgatgctat tttatcaaat cggcgagtat ttccaaggat tggcagtcag tcattcaaga
    10441 aaatcgattc gcgaattgat ggcgatccgt ccagaaacag ctcacgtgca gacggcggaa
    10501 ggattgatga ccgtgaaccc tgaagacgtg ctgatcggtc agtttgtact tgtgaagcct
    10561 ggtgaacgtg taccgttaga tggaacgatc atcgaaggag aatcattagt cgatacgtca
    10621 gcacttacag gagaatcagt ccctaaatct gtatatgttg gtgaaacagt attaagcgga
    10681 tttatcaata aaaataaacc atttttagtt caagtagaaa aatcttatga aaattctact
    10741 atcagcaaat tattagaatt agtagaaaat gccagcagta aaaaagcacc agctgaaaat
    10801 ttcattacta aattcgcgcg ctattacaca ccagtcgttg taggattggc cgttttatta
    10861 gctgttttac ctccacttgt tgtttctaga gcggcgtttt ctgaatggat ctatcgagca
    10921 ttgacatttt tagtgatctc atgtccatgt gcattagtga tttccgtgcc acttagtttc
    10981 tttggcggta tcggaggagc aagtaaaatc ggtgtattag taaaaggttc aaattacttg
    11041 gagttgcttg cacaaactga gacagtggta ttcgataaaa ctggcacatt aacgaaaggt
    11101 gacttttcga tccagacgat cgatccaaca ggaattgatc caaagaaatt tatgcaatat
    11161 gttgcaagtg cagaacagtt ttctacacat ccgattgctc aatctgtttt agaaggctac
    11221 gcaggtccgc t
//
LOCUS       DACEPW010000082        10976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEPW010000082 DACEPW010000000
VERSION     DACEPW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.083"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(122..808)
                     /locus_tag="IW819_001659"
     CDS             complement(122..808)
                     /locus_tag="IW819_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1244763.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(944..1138)
                     /locus_tag="IW819_001660"
     CDS             complement(944..1138)
                     /locus_tag="IW819_001660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244764.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(1188..1628)
                     /locus_tag="IW819_001661"
     CDS             complement(1188..1628)
                     /locus_tag="IW819_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1244765.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(1632..2477)
                     /locus_tag="IW819_001662"
     CDS             complement(1632..2477)
                     /locus_tag="IW819_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1244766.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2626..4044
                     /gene="pepV"
                     /locus_tag="IW819_001663"
     CDS             2626..4044
                     /gene="pepV"
                     /locus_tag="IW819_001663"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1244767.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(4101..5048)
                     /locus_tag="IW819_001664"
     CDS             complement(4101..5048)
                     /locus_tag="IW819_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1244768.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(5271..5621)
                     /locus_tag="IW819_001665"
     CDS             complement(5271..5621)
                     /locus_tag="IW819_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1244769.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5821..6900
                     /gene="pepA"
                     /locus_tag="IW819_001666"
     CDS             5821..6900
                     /gene="pepA"
                     /locus_tag="IW819_001666"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1244770.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7044..7364
                     /locus_tag="IW819_001667"
     CDS             7044..7364
                     /locus_tag="IW819_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1244771.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            7386..7850
                     /locus_tag="IW819_001668"
     CDS             7386..7850
                     /locus_tag="IW819_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1244772.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            8055..8660
                     /locus_tag="IW819_001669"
     CDS             8055..8660
                     /locus_tag="IW819_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1244773.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(8699..9988)
                     /locus_tag="IW819_001670"
     CDS             complement(8699..9988)
                     /locus_tag="IW819_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1244774.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            10416..10895
                     /gene="rlmH"
                     /locus_tag="IW819_001671"
     CDS             10416..10895
                     /gene="rlmH"
                     /locus_tag="IW819_001671"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1244775.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         3440 a         2084 c         2166 g         3286 t
ORIGIN      
        1 aataaacggt gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg
       61 aggaactatt ttgccgtttc tttctctaat actcaggtca aaacgctgat tccaaaacct
      121 cttatttgat gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt
      181 attcttgctc ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa
      241 agttatgatc aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt
      301 ctaattctga aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt
      361 ctagccaatc ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag
      421 tatccaaatc aaaaaaagct tttttatttt gagcgattac tgcctgatcg gtttgttcta
      481 gtattttttc tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga
      541 tcggtttttc caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt
      601 ttgaaacata agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga
      661 tcagtgcttc tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg
      721 agattttact tccaggtgct aactcggaat aaatgatttg tttacgtata gattcataag
      781 cttgctgttg caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat
      841 tataacataa atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc
      901 tgataggcat gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct
      961 cttggatcgg tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt
     1021 ctttgaaaat gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga
     1081 tgtaaccgtg cattacttta acgacataca tggggatcac ctgctttttt cttttcatta
     1141 tacgaactac tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca
     1201 tcatccaaag aagcgtacgc ttggaataac tgatctaact ccggattgac tttgctcagc
     1261 ttcactaatc gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt
     1321 aattttttgt cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt ccccgtatac
     1381 ttttggatag ttggatggat gctcactttt tctccgtaat ggatcatttc tttatagctg
     1441 caatttactt ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac
     1501 cctagatgta ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga
     1561 tgaataatcc ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat
     1621 ttctccatga ctcatgcctc gttttccatt gattttttac aaaaataatt catccatttc
     1681 ggaagtgctt cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct
     1741 ccaatgaaac tatgaagata aatagctgct gcaatcgttt ggtcattttg aggaaactgg
     1801 gctaaaaatc ctgtgatcat cccagctaaa gtgtcaccca tgccgcccgt tgccatagca
     1861 gcagaaccta aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt
     1921 ttcaaaacga gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt
     1981 tcttgtatcg gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt
     2041 tgttcgggat agggtagcaa aagatgatta gcagcaagta aacttaaagc agagccatca
     2101 atcaccagcc attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc
     2161 ttagaagaag tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa
     2221 agaaactggt cagtatccgt ccaaactaca atcattgctt ctggcagatg actgtgaaga
     2281 gcactccgat ttttttcatc agttactaca gtcgtgagac ccgctccgct gtgtacggca
     2341 gctaaagcac tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata
     2401 cgtccaaatg ttcctttatg tgaaattttt ggacgaggtg tgatgactct tttcactagt
     2461 tgttcgttta tcagcatcta aaaacctcct tttactcatt atagcgtata ttttttttag
     2521 agaaaaacag ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg
     2581 tggtatcatg aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg
     2641 caaaaagaag tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt
     2701 gtcaacagcg agcgcgatga ctcaaaagta acaccagatg caccatttgg acctggacct
     2761 cgcgacgcat tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat
     2821 gtcgataact atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt
     2881 ggacacatgg atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta
     2941 atcaaggatg gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca
     3001 gcttactatg cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc
     3061 ttcgtagtcg gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat
     3121 gaagaagaac cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa
     3181 aaaggaaatg tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg
     3241 aaatcttttg tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt
     3301 caagtaccaa gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa
     3361 gcaaatccag taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt
     3421 ggtaaaggcg cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca
     3481 ttgttcttag ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct
     3541 gcttatgtcc atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa
     3601 atgggcaaat tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa
     3661 gcgaacttca tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa
     3721 agtgacatcg aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta
     3781 atggaaccac attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat
     3841 gaagaccaca ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt
     3901 ttgttgaaac gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat
     3961 caagcaaacg aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat
     4021 gccatctatc gtttggcgaa ataattaatt aaaaagagga tcaagaattg caaattattt
     4081 ttgcagtctt gatccttttt ttactttcta ctgtggtatt catgataaac atgatataga
     4141 ttcaattcat attctttctt agcgttcgtg acgactgtat ccaaaatcaa ccctgttact
     4201 aatgaaagaa gtgctaaagt catcaatcca gtacttaaaa tcgcagaagg gatgcgtgta
     4261 ataaagcttg tcagcatata ttcgcgaatc acaggaatcc ccgtgaccaa tccaaataaa
     4321 aagaagaaga tggtccaaat cccaaagaaa agcaaaggct tataatcttt aaacatttta
     4381 ataatcatca tgatcacttt gaatccgtca gagaatgtat tcaattttga ttcgctgcct
     4441 tcaggacgat cacgataatc gattgggatt tcaacgaact tgaatttttt atctaaagca
     4501 tggatcgtca attccgtttc gatttgaaaa ccagaactca taataggaaa acttttgaca
     4561 aagatacgat tgaatcctcg atagccggtc atgacatcaa gaatttttgt tttatacatt
     4621 ctagtaattg tatttctaac aaggttattt ccaaaatcgt ggaaaggtct cttattctca
     4681 tcaaagtagg ttccatttga taggcgatca ccaatgacca tatctgcttc accagaacgt
     4741 aatttctcga gcaaaccatg tacagcttct gcaggataag tatcatcgcc atctaccatt
     4801 aaataatagt cggcatcgat atcaaagaac atttggcgga tcacatttcc ttttccttga
     4861 cgaggttctt tttttacgat tgctccacct tgaacggcca attcgtatgt tcgatcggtc
     4921 gaattattat catagacata aatatctgct tctggtaatt ctcgtttaaa gtctgcaatg
     4981 actgttgaaa ttgttgcttc ctcattgtaa caaggaatca gaacagcaat tttttcttgt
     5041 tgttccattt ttcactctcc ttaactatag aaaacaaaag ctaaattaaa aatacctcat
     5101 aatcaaagga aatgctagtt tttcccgcta aatatcattt atttaaaaag aaaatactgg
     5161 aatatatgtt tggaaagctc tatctaaaaa accacccact acaaaagaag gacatgatac
     5221 ctctcttttg aatagtgggt gcgtattcgt tctctcactt ttttgacgta ttaatctgtt
     5281 gcaccttttt ttcttgaaat ttctaatacg gttcctgtat gagcatctgc taaaaattca
     5341 taataaacag tttcgccatc ttctattcga gaaagacctc cccgataagc actcgaccga
     5401 acggcgaatt tcctaatagg ttccttttca aaagagatcc aagaaccttc gattgcacct
     5461 tcttttagaa aagatgactt gatattttca agtacttggt ctgctgacaa tacttttttg
     5521 cgatgatacc aaatcgttga agctaccccg ctcaataatc ctagaccgat tccaactgct
     5581 aacccgcctt taaaatagct tagttcgctt tcttcataca tgtggacata cctccttctt
     5641 ctcacttgtt actccttgtg cttattttac cacactctca tattttcttt gaaataaata
     5701 accgctacca gaaaaaataa gctataataa atacgatcgg tctgcagaaa aaatcatatt
     5761 ttatccgcag atcaatataa gtctgagagg agtttagtct gaaatttaga cgaatagcat
     5821 atggaagaaa aaacatttca acgaatcaaa gagttaacag aattacaagg aacaagcggt
     5881 tttgaacaag atgttcgcgc ttatatggaa agtcatatgg aaccgctggt agatgagtta
     5941 cagttagatg gtttgggcgg catttttggt ctgcgccatc atgaagaagc ggatgcaccc
     6001 cgtgtaatgg tcgcagcaca tatggatgaa gtcggattca tgctgacaca aatccaagaa
     6061 aacgggcttt ttaaagtcgt acctctaggc ggctggaatc cttacgttgt ttctgctcag
     6121 cgttttacac ttaaaacctc tactggtaag aattatccat gtatttcttc ttccgttccc
     6181 ccacatttat tgcgaggaac aagcggacaa aaatcagtag aagtaaccga tattttgttc
     6241 gatgctgggt ttgaatcaag agaagaggca atgagttacg gtgtccttcc aggagacaca
     6301 atcgttcctt acgctgaaac gatcaaaaca gccaacggaa agaatattat cagtaaatca
     6361 tgggacaacc gctatggctg tacaatggta ttagaagctt tagaagcgtt gcaaaatgaa
     6421 acattaggtc atacattgat tgcaggcgcg aatgtccagg aagaagtagg cttgcgtggt
     6481 tctaaagctt ctgtcaataa attcaagcca gatctgttct ttgcagtaga ctgctctgca
     6541 gcagatgata cagtgacgaa aaatggcaca ttcggacatt taggagaagg tacattgatg
     6601 cgtatccaag atccaggttt gatcatgctt cctcgtttgc gcgagtactt gctggatatc
     6661 gctgaaacaa acaatatccc ttatcaatac tttgtttcaa aaggcggtac agatgcaggt
     6721 gctgctcata cacaaaacga aggaatccca agtacagtga ttggcgtagt gggacgttat
     6781 atccatacgc atcaaacgat gttcagtatt cgcgattttg aagctgcacg agaaatgttg
     6841 attcaaacac tgaaaggttt ggataaatca acagtgaata cgatcgtata cggaaaataa
     6901 tgcgatagtg aaactataaa atctggcaaa agattgcttt ttgccagatt ttttttataa
     6961 aaagaggatc aatgtttgtt agttcgaaag aaaggtgtat aatttaattg aaaaatacat
     7021 aaacagaaag tagaggacaa agaatgatta tcccaaatag tttagaagaa ttagcaggct
     7081 acgtagaaac tggaaagagt gtatttttct ttactgccgg ctggtgcgga gactgccgtt
     7141 ttatcaaacc gcaaatgcct gaaattgaaa atgacttttc tgattggcaa tttattgaag
     7201 tagaccgtga caagtacata gatgtagccg ctgaatggaa catttttggt atcccaagtt
     7261 ttgtcgtgat ccaagacgga aaagaattag gtcgtctagt gaataaagat cgtaaaacaa
     7321 aacaggaaat cgaatcgttc ctagaaagtg taacaaaagg ctgacatctg ttaggagggg
     7381 tcaagatgaa tcaagaatat aagaatattt tagtaggtat cgacggtagt gaacaagcac
     7441 ttgctgcttt caaaaaagcg gtagaagtgg ctcgccgtaa taatgggaaa gtatatgtag
     7501 caaacgtaat cgatcagcaa ttttataact ttatgggcta tgcacctatt gatcaaaatc
     7561 tagtcgatca gcaaactgaa acagccaagg aaatgatcga agaatgtaaa gaatatggga
     7621 aatctgttga ttacacaaat atagaaggaa ttattgcata tggttctgtt cgtgaatcca
     7681 tggcagtcaa attgccaaaa aaatatgaga tcgatctaat catggttggg cagtcaggtc
     7741 taaatgcagt cgaacgcttt atgacaggaa gtgtagccag ctacgtgata cgtcattcac
     7801 cttgtgatgt cttgatcgtt tctgacgaag aagaacagcc aagtaattaa gcaagtggaa
     7861 cttgtacgac ctttttaatc aattaactaa aacagtaaag ggactgtgac aaaagtcttg
     7921 tcacagtccc ttgttacgaa taaatggtgt tccagaagag gcttcttcgg aaataaagca
     7981 tgaaaaaaat ttcatgcagt gaatgcaatt ttttgttaag ataaatctca gcaaaggata
     8041 atggaggaaa gaacatgatt tttgcttata acaaagcaca cgtcggagac accttgatgg
     8101 ttattatagc cgacgataaa ggaacagaaa acactgttga acgcaaatgg aatgttgctc
     8161 ggatcaaaga tgaacaaggt caagttgttg cttggaactt tttccatatc tccgatcatc
     8221 taacaatcga agggaacggc caagtaagct taacagatga ggaattagcg aaattgaatc
     8281 gattgatcaa agaagcagga ttcgaagaac agcttgaagc tgatcatgaa ccgaagatcg
     8341 ttgtcgggta cgtaaaaaca tgcgtcccac atcctgattc tgaccattta tcgattactg
     8401 agacagaagt agacaatggt acagtattgc aaatcgtttg cggtgcaccc aatatcgaag
     8461 ctggacaaaa agttgtcgtt gcaaaaccag gagctatgat gccagatgga atgatgatct
     8521 ggccaggaac tttgcgaggg gtagatagct atggaatgat ctgttccgct cgtgaattgc
     8581 atttgccaaa tgctccagca aaaaaaggaa tcttagtatt gcctgaggat gccgtagtcg
     8641 gagaagcatt tccaaaataa agaggttgtg acaagtcttt gtcacaacct cttttttatt
     8701 aattttcact attttgattt tgttttaatg ccgattgatc gatcgtcaat tcaacagtag
     8761 ttgttttttc tttcgagcct tcatagtaag tgattttgac agaatctccc acttttttct
     8821 tatacaaagc agattgaagt tctacaccag aagaaacttc tttgtcatcg atcttagtaa
     8881 tgacgtcgta ttgtttcaat ccagcttttt cagccggtgt agctgtttgg acatttgtca
     8941 cgatgacacc gttagttaca gatgaaggaa tcttcaagat ttgttcttgt tgctgagtag
     9001 aaacggcaga taagtcgacc attgtgatac ctagagctgg acgtgtcact tttccgtctt
     9061 tttccagttg attgatgata ttcactacat cgttacttgg aatagcaaat cccattcctt
     9121 ctacgcttac attagaagta gattcagaag tacttgcgat cttacttgag ttgataccga
     9181 taacttgtcc ttcgatatta actaaaggac caccagaatt tccagggttg attgctgcat
     9241 ctgtttggat cgcattgata ttgactgttt cgttcgattc attcgtactt gtcacttgtc
     9301 ggttcaagga agaaatgatt cctgaagtga cagaatttgc atattcagat cctagtgggg
     9361 aaccaatcgc aatcgcaggc tcaccaacct tcagagcact tgagtcacca aaagaggcaa
     9421 ctgtttcgac tttgtctgaa ttgatcttca acaccgctaa atctgaataa gcatctgttc
     9481 caactaattc agcttttacc ttcgtaccgt ctttcatcaa gacttcaaga ccttgctggc
     9541 catctactac gtgattattc gtgactacat aagcagtatt cccgtctttt ttgtagatca
     9601 cgccgcttcc ttcactataa gcttccaaat tactatcatc tgagctgcta ctttcttctt
     9661 gctgcccgaa tagtccgcca aaaccgcttg attggttttg actttgtaag ttgataacag
     9721 aaacgacagc gtcttgaact ttatctaccg ctttggtgat atcagaatct acattgactt
     9781 tgacattttc cacaactgtt tcaccggcag aattctgatt gccgcttgta gcagtagaac
     9841 tattgttgcc agtgcccatc gctgcataaa aaatgccagc tgtcagtagt cctccaacga
     9901 tccctccaac aagccccaga cctaacttac gccagaggct attattttta ctttttttca
     9961 tctttggtgt cacatttttt ctatccatgt gttcctcctc caattacatt catttatcta
    10021 ctgttagtat agtcttattt gttgacttta gtctactttg agactgtgaa ctttttatga
    10081 aaaactttag gaaaaatcat tgttttcaga tgaaaatatt tgattttctt tttccacaga
    10141 tactgtgtaa aactacgcgg ataggattgg aaaaataagg gataaaacat atccacagcc
    10201 tgtgtataaa gtggataaat tcttataaaa cgtatgtttg cctaatgaat gtgatgaaat
    10261 aaggtaaaat aacactgttt tctgtggata aacctgtgga tgttgtgcat aactgttggg
    10321 gaaatctttt taacaaaaat agccaaaata aacatataga cgaaaaaatt gattataata
    10381 aacaaagtga gcatgaagac caaaggagag aaaacatgaa tatcaaaatt atctcagtag
    10441 gaaaattaaa agaaaaatac ctgatccaag gtatcaatga gtatgtcaaa cggctgaatg
    10501 cctatgcaaa aatcgaattg atcgaagttc ctgacgaaaa agccccggag aatttaagcg
    10561 aagcgcaaat gagacaagta aaagaaaaag aaggggaacg gatcttagcg aaaatcaaag
    10621 aacaagaata cgtctatgcc ttagcaattg aagggaaaaa tcctacaagt gaatcttttg
    10681 ctaaacaaat cgaccaatta ggtatccaag gaaaaagcca ccttgttttt gttatcggcg
    10741 gctctttagg actcagcgaa gccgtcatga aaagaagcaa tgctcagatc tcttttggaa
    10801 aaatgacgta tccacatcaa ttgatgcgtc tgatccttgt cgagcaaatc taccgtgcct
    10861 tccggatcaa tgcgggggct ccttatcata agtgatgcgg tttttatcat tgatcctaaa
    10921 aatcttagca agacaaaaag tacgtaagaa agtaaagact aaatacttcg gaggaa
//
LOCUS       DACEPW010000083        10740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEPW010000083 DACEPW010000000
VERSION     DACEPW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.084"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1156..1554)
                     /locus_tag="IW819_001672"
     CDS             complement(1156..1554)
                     /locus_tag="IW819_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244776.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGNYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1770..2825
                     /locus_tag="IW819_001673"
     CDS             1770..2825
                     /locus_tag="IW819_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ1244777.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2845..3198
                     /locus_tag="IW819_001674"
     CDS             2845..3198
                     /locus_tag="IW819_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244778.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3380..3676)
                     /locus_tag="IW819_001675"
     CDS             complement(3380..3676)
                     /locus_tag="IW819_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244779.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3680..4498)
                     /locus_tag="IW819_001676"
     CDS             complement(3680..4498)
                     /locus_tag="IW819_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1244780.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4724..4945
                     /locus_tag="IW819_001677"
     CDS             4724..4945
                     /locus_tag="IW819_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAQ1244781.1"
                     /translation="MPVISIRVSEEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4947..5213
                     /locus_tag="IW819_001678"
     CDS             4947..5213
                     /locus_tag="IW819_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1244782.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5326..5865
                     /locus_tag="IW819_001679"
     CDS             5326..5865
                     /locus_tag="IW819_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244783.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            6071..6253
                     /locus_tag="IW819_001680"
     CDS             6071..6253
                     /locus_tag="IW819_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAQ1244784.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            6356..6640
                     /locus_tag="IW819_001681"
     CDS             6356..6640
                     /locus_tag="IW819_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244785.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6647..6892
                     /locus_tag="IW819_001682"
     CDS             6647..6892
                     /locus_tag="IW819_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244786.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAELDAIGNIHFS"
     gene            6914..7168
                     /locus_tag="IW819_001683"
     CDS             6914..7168
                     /locus_tag="IW819_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244787.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7250..7435
                     /locus_tag="IW819_001684"
     CDS             7250..7435
                     /locus_tag="IW819_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ1244788.1"
                     /translation="MPKTSREIIKILKSNGFKKTGQTGSHAKFYNSKTKKTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7502..7897
                     /locus_tag="IW819_001685"
     CDS             7502..7897
                     /locus_tag="IW819_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ1244789.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKTSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELFEK"
     gene            7887..8264
                     /locus_tag="IW819_001686"
     CDS             7887..8264
                     /locus_tag="IW819_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244790.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKQAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8265..8660
                     /locus_tag="IW819_001687"
     CDS             8265..8660
                     /locus_tag="IW819_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244791.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8966..9364)
                     /locus_tag="IW819_001688"
     CDS             complement(8966..9364)
                     /locus_tag="IW819_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ1244792.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9605..>10740)
                     /locus_tag="IW819_001689"
     CDS             complement(9605..>10740)
                     /locus_tag="IW819_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ1244793.1"
                     /translation="NLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKELASQS
                     SLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQTNYN
                     THYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPNTDIL
                     VRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREVQYFS
                     TTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGTMENF
                     IKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHSIRLK
                     FLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT         3679 a         1843 c         1724 g         3494 t
ORIGIN      
        1 acttttgttt tgtgttaagt ttctttacat tactacgcac attgcgtgta aaaattcttt
       61 atccatccca cgctccatgt tattttatat ttcctttttc atgttatttt tcgtcagaaa
      121 ccgatctagt ttttacctat tcccaaaatc catttattcg ttatttttta ccctgattaa
      181 ggataattat ttgatgctta aattatctaa aaaagcgatt tgttgccgat ataatcatga
      241 tattaaagct gaattaggcg gaaaggcaaa atgttcgcaa aatcgaagta tcaattatct
      301 tgttttttta taaattttta agtgaaaata tgaaaaattc tcttttaatt ggtataggta
      361 ttaaaattac atttaaccat tcataaaagt atgcgctttc attgtttttt atgagaataa
      421 tatcaataaa taacgatata aaaaccattt tttatactgt tttataagaa tataaattga
      481 tctaatttag aaaaataatt caaaatttaa atgttttaat attacaaata gaataatgtt
      541 atcgaaaaat ggtataaaac aggtgattat tttatttttg ttattttatt agcaggaaca
      601 tctaaataat tttacatgag accgtagaaa tatattggtt tcaccttcag attaatattg
      661 gatcatatta atctgaatat aatatatgaa ctaataaata gcttaacctc gctattttgt
      721 tttttctgat cactacctac acagcattag taaatagaat ttaaaaaata aaaaaattaa
      781 gacaaaaaaa gtatcaacat aaacatatac atatacacgt ttatgttgat acacttaatt
      841 tgtcctttaa aaagcattaa ttattgaatt atgttatttt ataatttgat tacaaactga
      901 ttgttaggtg ttgtccttga attacatttt ccaacggtta ttttatcgtt taatttatta
      961 attatttttg atctaaaaat tagcttaaca aatatataat taaccaacca ccagataaaa
     1021 atgcgatcat tgctaataaa taagcaaaga taaaaatatg ttcgatcagc ttaaaccctg
     1081 atgcgattaa gcagaccaag taaaatagaa attctcttac agataatggt aattttctaa
     1141 ctagcatact cataattaac accccaaagt aatattgtat tttgaacatt ttacatccaa
     1201 tacagcatca atcaaccatt gccaatcaaa aatgcaaggc ttcattgaag tatgtttttc
     1261 agatgaaaag atataatata atttcttcgg aataatattt tcagttgttg ggatcaagct
     1321 aaatttaata ccatctaact catagttacc agctttctct ccattaaaaa tttccttagc
     1381 actttcttct aaagaatcaa aatatttttc gacagcaacc tttgaagctt ttactattga
     1441 agcatttccc ttttcgataa aatctgcttt tgttttaccg acaataagta attcttgatc
     1501 agtaaaatct tctaaaataa tagtagtgta attcttattt cttctcaatc gcatcaggat
     1561 cactccttaa tataatttat gtttttataa tatcataata agataaattt gcaagtgttt
     1621 atttacacct ttttacttac actttattgt aattgattat acttttttga ttgcattttt
     1681 ttattataaa cagttgcaac tattagtaaa taggtgtaaa ctattttcgt accgaaatac
     1741 cataaacaca aacagaaaga ggtcatacca tgaaagaacc attattgttt tccgttgact
     1801 tagggaataa atcagtaaaa gaaatgagta cacacgtttc tccactctcg cttccctctc
     1861 gtattttgaa caaaaatatc attaattcag gacgtttttc taatataggt tcctcattaa
     1921 aacgatcact aaacgtttct gaatatagcc tagtaaacac tactgaaagc tattattttg
     1981 ggaaggatat acacgagcta ggaaaagatg actacgtgat agaatcacta ggttttggga
     2041 tcagcagata caagcaggaa acctaccaaa atatcctatc tttctcttta gctaacctag
     2101 cacgtttaga agatcaatct gatgacgttt taaccgttaa tctagtttta ggtctgcctt
     2161 catccgacta taccgaaaca atcgtcaaat acgtgatgca acaagccaca cgacaacatt
     2221 cagttgaagt tgacggtaaa gcttataata taaacgtaga taccgtttgg gtactgccac
     2281 agccgattgg aacactatat aacctcctcc ttgctgatga tggtagtgta caagatgaat
     2341 cattgatcag tcagaagatc ggttttgttg atattggtgg tggcacgaaa ctttttgatg
     2401 ttgtccaaaa ttttgaaatt gacgaaaaca accgtgaaca aaaggatacc ggtattttta
     2461 ctttatataa tcgtatcgta aatatgcttg accttccgat cagtcctaat gctttccaac
     2521 ttgaaaagat tatccgagaa ggcaaagaaa aaggcgtata tctatttaag ccaagcggaa
     2581 atgaaagttt tgatatttca gaagtagttg aaagagaaat aaaaacatac accaatatgg
     2641 ttgttgcaga agttttagct acattcaaaa acttcacaac cttagataaa gttgtcttta
     2701 ctggtggtgg ggcttctatt atcgacaaac aacttgtaaa agataaaatt ccgcatacct
     2761 tgtttgttga agacagtgaa ttttctaatg taaaaggctt ttacaagttt ggtttaactc
     2821 aataaaacag cgaaggagat caacatgaat aagggaaaat cagagcgtta tgtagttata
     2881 atcaatagag aaaaggttag cgagttacct ttagcagagt ttcttgaatc tgaatatgaa
     2941 acactaggcg ttaaatactc tcagacgatc aaaaaagctt tgaatctata tcggcgtgta
     3001 aagaacaatg aagttacaat tattgaagca ggcaacgata gaaacgatag caaagtagca
     3061 gaagtaaaga ataaaccaac tgatcataac cctattcaac aagagcatcc ttcaacggtt
     3121 acatccgatc attcagcaaa gaaagaaaag ccaatgctaa aagggttcaa tgcaccaagt
     3181 aacaaagact tactttaaaa ataaaaaggc gaggttacaa tacctagcct ttttattatg
     3241 tacaaataca aacataaaca tatacgtata catgtatata aacatgtata cgtatatatc
     3301 cataaacacg tatacgaaat ttagttaagc tatacctaac cgcttattcc aataaaaaaa
     3361 gtgatcagct ttaaactgat cactcaccaa aaacttcatc atacaagtta tctatcattt
     3421 caccaagtaa agctgaatct gatcgaattc ctaacttttc gctgatttta gatagtttct
     3481 ttctttgtga tggttgaagc gtgaattgat aatttttctt cttttcagaa ctatcacttt
     3541 ttagtttagc taccaaagaa cttgttgttt ctttaggtgt ttgtttaggt tcttcaactg
     3601 cttcaaccac ttcaggaact tcttctactt cagcttcagc gaatttttta aatctatttt
     3661 ctttcacttc aatcactcct tattcttgtg ccgtatcaat tattttgttg tatagcgtaa
     3721 acgtgctttc aaagaaatcc ttattcttac gatagatatt cttatcctta gcgaactcag
     3781 caatattctt accttctaag ctagttgcat taagcaattc cttctcttgt acatatccaa
     3841 gataattaga taacgttggt accaattcta ggaactcacg acttgaatta gtattatgtt
     3901 tgatcctgct accgatcgta gcgtatttag tttccatcag tgtttcacca gtcttaatat
     3961 caatcaactg ttttgctagt gcatcaatat ctttaccaag cttcagcaaa gcttcaaaac
     4021 catctttaga tggatcagaa atgcctacga caagatcaga tacagcccaa gcattttgag
     4081 ctagtagacc tgcatcatta tgagtatcaa tcagaattag atcatacttc tctttaagct
     4141 gttcatagtt aacaacgatc catctaaaca gtagggttct gttgttaggc atatcacgca
     4201 tttgcatctc aacttgtttt aaatcttcac ctgctactaa aatatccatg ttctcattta
     4261 tagctaaagg ttcagctgat ccatgaagat ataaatgatt gattgtattc tctcttttcg
     4321 ctttactgaa agcatcagga aatcgttttg ataggttttg cgacttatct tcatcaatat
     4381 acagcacctt catacctaaa ttttcgacag catatccacc tagattgtag attgttgttg
     4441 ttttacctgt tccacctttt tcaatgtttc cagtaatgat tgacaacttc ttttccatga
     4501 ttaaacctcc cttattaacc ttttgtaact ccattataca ctataaacac atttatgtat
     4561 atgtttatat acataaatgt gtttatattt atatttgctt ttatattcca caacataaac
     4621 atatatatgt atatgtttat gtttatgtat atattcaaaa atataactat tgcatttgtc
     4681 atacaatcgt agtacaatgt aattgaaaag gagttgataa actatgccag taataagtat
     4741 tcgtgtatct gaagaagaaa aagattttct tgaaaaagtt gctgaattca acggagataa
     4801 catttctgaa tttatgcgta gtaattccct gcaaacagca gaaacgttag tagactttgc
     4861 cacctacaag caactaatga aagagcatga ggaaaacgat caaagtattt cccacgaaga
     4921 tatgctaaaa gagttaggct tataagatga cttaccaagt aagatatgaa cgtgaagctc
     4981 aaaaagcgct aaaaaaaatg gataagtttc aggcaaaggt tatcctaaac tggatcgaga
     5041 aaaatcttgt aggcactgat gatccaagaa agcatggtaa aggtctaaca gctaataaat
     5101 caggctactg gcgatatagg gtaggtgcat atcgtataat agctgatatt agcgagcatg
     5161 aagtgacaat tcttatattg tctattggtc acagaagtga agtttataag taacagacaa
     5221 gctgataagg cttgtctgtt tttttgttaa cccaacaaaa tcactgttta cgatcccact
     5281 ttacctaaaa aagaacaata tcctcttgat attatacaca ccggtgtgta taatataagt
     5341 gtaaacaagc taattaactt tcaaaaggag cgatttttta tgggatggta tggtgattat
     5401 tcatcagcaa atgaagtgat cgaagaaaag aaagggtacg ccaaaaatgg tttggaaatc
     5461 gtagatacca aagtaacgaa gacctatgga attatccttt tcaaagatgc agaaggaacc
     5521 tacgatattg acagcttcat ctttcgagat gaaatgtaca aaccaatcta cttcttaaat
     5581 gacagtcctg cttactttaa gaaaattccg aaaaaatggt tgaaattctt tgtcggtgat
     5641 caagaaaaaa tggttaagcg acaactagat agaattaaag aagaaagcaa taaaccaaaa
     5701 cttgatgact tattagttgc aggtaaaact tactcaatct gggatggatc gtatgtcgct
     5761 gtctatcagt ataaactcaa acgtagcttt atttttcgac tacaagatgg atcactcact
     5821 cggttcactc gactatcagc aggagatgtt aaggaagtag cctaaaactt aaatacataa
     5881 acataaacgt gtatacgttt atgtttatgt atatgtatat gaatataaac gtattaggta
     5941 cgaacacttt taaattcttt tctgggcttt gtttttaggg tagggctaaa aacatcacca
     6001 tctgatccaa aacagctcag aaacgcattt aaacgtatct gataccaata ttacagaagg
     6061 agtgtatcgt atgattaacc tatttctttt agtgacagct agtacattat tctttactgg
     6121 aatatcactg ataatttcct atttgatcta taagaaagcc gaaaatcaca caagcaaagc
     6181 aaccggacta gccgtttcat cctttttaac tttgctgact ttatttgtta ctttatcgtt
     6241 ttcttttgtt tagaacagct gatccattag cacctttttc tctttacatt atacacaccg
     6301 atgtgtataa tgtatttgta aacaagctaa ttaactttta ggaaggagtg acaagatgct
     6361 aacagctact ttacagcaac ctactcacgt tgctattgca gaaagttata caattaatgc
     6421 agggatcgtt aaacagctga tcaaggatcg agaattcaaa aagtttaaca gtcggcaagc
     6481 gtataacaga cagctaaaga aagctattct aaagcacaaa gattccttca tcgggaactt
     6541 ggaaagtgta gatgttaaaa ctgtttcaac ggcattttca gttgttgaag tgactattga
     6601 cggtcgcttg atttctctat ttgcttttgg aattaattga tagggagtgt ggactttgga
     6661 aggtaagact tttgcagaaa tttggactga atttattgaa gtaccaactc gcattgatcc
     6721 gataacttct gaaaaggtat tggatgcaga cttctacaat ctcaaagcag gtactagcct
     6781 tgaatgcgtt tggatgtatt tagaagaaca tttcaaaggc gtaacgctcg gtcacgtcct
     6841 ataccacaat ggtgccgagt tggatgcaat tggaaatatc catttttctt agtacaagaa
     6901 aggagaacac aatatgaatt ataccgatga agaacaaatc actttagaat atgttttcat
     6961 agatgaagaa ggggaggaaa ttgaaaaagt cttgattatt tcatcaaaag acttggagaa
     7021 cgctttgact tatgaaccat acgaatcaac cgtaactgtc aatgaattct tagataccta
     7081 tacttctgat gatacttggg aaatcatgaa tatttgcaac gctaataaca ttcaatttgc
     7141 tgaaaaaacc aataaatcat tttattagtt ttgcaataaa acatcctctt tttctattga
     7201 ttccatacac aacaatgtgt ataatgaatt ttgtaaaggg agggtagcta tgcctaagac
     7261 atcaagagaa attataaaaa ttctcaaatc taacggcttc aaaaagactg gacaaactgg
     7321 cagtcatgca aaattttata attccaagac taaaaaaacg gtcgttgttc cgatccacaa
     7381 tggtgatgta ccactaggaa cagaagctaa tatctggaag caagcagggc tttaacagcc
     7441 ttgccttcct atttggtatt aggcatacta tcctctctta cactagaaaa ggagcgagga
     7501 aatgaagaca aaaaatattt tttatccggc aatcttcgag aaagaaggag aagcttataa
     7561 catcgttttc cctgacttac cggaaatatc cacctttgga aatagtcttg aagaagcata
     7621 tttaaacgct aaggatgctt taggattagc gctatatagt gtaccagatg cagaatttcc
     7681 taaaacatca gcagtggaag aattaacttt gaaagccaat caagtggtag taatcatcca
     7741 actcaacatt aaacttttcc gaagaagctt aaatacaaaa acaattagaa aaaatgttag
     7801 tgttccggaa tggttagttc ttttagggaa ggagaaaaac ataaacttct ctcaactact
     7861 tcaaaaagca ctagaagaag agttgtttga aaagtaaaaa ggagaccgaa cctatgaaag
     7921 ctacacactt aaactatttc tctcgaacgt taatcaataa tctattaaaa gatacaactg
     7981 gcattagcgt tagtaccaaa cactttgcca atgatctaat tatttttgag gataataact
     8041 tgcgaattga gatgtttatt gaacaggcaa cccctaactc aatacctgaa ttctgctata
     8101 cgcacactga aaaaatgaaa caggcaaaat cagttaaaga tgttgaagca ttaagtgaat
     8161 cactaacgaa cgctttaaac ctcgttaaga aggcaaaatt gttatttgaa tttcaaaccg
     8221 actaccacaa tgagttgctt gctcaatctg aaattttaga ttaattgaaa caggagaaga
     8281 gaaagatgaa acaaacacaa acagaaaccg ttaacgatac gcttgctgat caattagcag
     8341 gttattataa attatttgag atgagtaaag cacagcgctt gatccactat ctttggaaat
     8401 acaaaatttt gtttcttttt tgggaagcaa cactaacatt ctcagttctt ttttataagt
     8461 cagattttta taatcaacac ttagttttag ggagtttttt gcttatctac accattttga
     8521 tgcttaattt atatacctac atcacacttg aagaacactt agtaattagc gatgcaggga
     8581 tcgagagaaa catcaaaagg gtatctttca agatcacgtt aaacaagcct accgataaaa
     8641 agctctcaga agcgttctga gagcttttga cattttcttg tataatttat cctaacacat
     8701 tacaaagcac ctaattcgag cttatatcaa gcaaatttgc tatttattac tcaatacata
     8761 tctaaaaatt acttgatgga aaactagata catggtaagc atgaaaagaa aatcaatttg
     8821 agcagatgta gtacaataca tagcaattta aaagctctca gaaggcttct gagagctttt
     8881 aatatattct ttgataattt gtctttctta aatccatacg tcctaaaaca agctgttttt
     8941 gcttaatatg atcatctgag agacatcaaa tcaacttttc tctcaacaaa tcattcaaag
     9001 ctttcgacag attaatattc tcttcagccg ctaaaacatt taaatagtgt tccacagtca
     9061 ctgattttcg atcataattt tgcttgtttc gagctttagc aactttcgta ttagctttta
     9121 cgacaaataa attaacattc tcagatcgct tgattcttgc atttaactct tgaacaggcg
     9181 taggcgatgg aatttctttt tcttgcaact ctaaaagcac caagtatcct tccaatactt
     9241 cttgcgccat ctcaaaagca tcttctaaag tttcaccatg cgtatataag tctggaaaat
     9301 ctgggaattt aactgcatat tcatttcctt tccattcgaa tactgcacaa aaaaagtatt
     9361 tcatcgcatt gcacctctct aattaaaaaa taatttttgc tcctgaataa ttcatagctt
     9421 taattctttg gcacatttta ttgaaatatg tatcactaat atccctatca actgattttg
     9481 actaaaaagt tacttcaaac attcactgtt caaaaatatt gaggaataaa ttttgggtgt
     9541 aaggcatttt gagcatactt ctccctggat aacttcccag taaaaaaatc gttagattgt
     9601 tggattagat ccattgactg gctcgcctca ggcgagcaaa gaccctgtag aattcattct
     9661 gatacacatg ataactagat aatttgagat agactcgtct acccgtttgg actaatttcc
     9721 cagcaacttt cagaaacttc aatcgaatag aatgaatggt cattcccttt tggacttctt
     9781 caaagccaat cgttcttaag aagttgacta agttgtaagc tatcaagctg agcatcattc
     9841 ggacatgatt ctccaaaaag cgaggactgt ctgttttgtc aaagtaaaag ccagctttcg
     9901 cttctttaat gaagttctcc attgtgccac gtttggcata gatagaaaag atggtatcag
     9961 gagaaacatt ttctgataga ttcgtcacga taaattcatg tcgaaagagt agttcgcccg
    10021 cttcacgtgt tgagcgtata cacacgcgac gactttgtga ccaggattgt gcttgataag
    10081 tggtcgagaa gtactgaact tctcgttctt cccacttttg attatcgcca taaagtactg
    10141 atttctcagc tatttgacct agtctacgat tattcttcag tcgaataaca taatgactct
    10201 tttttgattc acacgaatca tacacaccag gtgtagcgaa cccgctgtct ccacgaacca
    10261 agatgtcagt gtttggtaga gagtgattat agtgctctaa taaaggtgtg agaaactcct
    10321 ttacgccttt tgatgtatat tgatttcctg aacgtagttc agcttttaag aagtcaccag
    10381 tcaatccgtc aaaagctacc aatggatggt aaccataggt ttggtagtgt gtattataat
    10441 tcgtttgttc ttggtgacca aatgtatctg aatgggtcga atctaaatca atgattaatt
    10501 ccgtatcatt acggatgagg cgtgctttat cgataagttc ttggttcaaa gcttgaagtt
    10561 catggatatt ctcctcagat agtcgatcta aaaatcggga aagtgaagat tgagaagcga
    10621 gttctttcct atctaaaaca gctttaaaca caggatcttg tctcaggaga ttagctgcag
    10681 aatcagctga gtaaccagca attaattgca taatgaactg ctcaagtatg gataagttgt
//
LOCUS       DACEPW010000084        10685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEPW010000084 DACEPW010000000
VERSION     DACEPW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.085"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(59..271)
                     /locus_tag="IW819_001690"
     CDS             complement(59..271)
                     /locus_tag="IW819_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1244794.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(292..990)
                     /locus_tag="IW819_001691"
     CDS             complement(292..990)
                     /locus_tag="IW819_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1244795.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(1010..1552)
                     /locus_tag="IW819_001692"
     CDS             complement(1010..1552)
                     /locus_tag="IW819_001692"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1244796.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(1684..2688)
                     /locus_tag="IW819_001693"
     CDS             complement(1684..2688)
                     /locus_tag="IW819_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1244797.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(2685..3674)
                     /locus_tag="IW819_001694"
     CDS             complement(2685..3674)
                     /locus_tag="IW819_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1244798.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(3671..4477)
                     /locus_tag="IW819_001695"
     CDS             complement(3671..4477)
                     /locus_tag="IW819_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1244799.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            4643..5599
                     /locus_tag="IW819_001696"
     CDS             4643..5599
                     /locus_tag="IW819_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1244800.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGPDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(5676..6194)
                     /locus_tag="IW819_001697"
     CDS             complement(5676..6194)
                     /locus_tag="IW819_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1244801.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(6282..6431)
                     /gene="rpmG"
                     /locus_tag="IW819_001698"
     CDS             complement(6282..6431)
                     /gene="rpmG"
                     /locus_tag="IW819_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1244802.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            6659..7105
                     /locus_tag="IW819_001699"
     CDS             6659..7105
                     /locus_tag="IW819_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1244803.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(7300..9195)
                     /gene="asnB"
                     /locus_tag="IW819_001700"
     CDS             complement(7300..9195)
                     /gene="asnB"
                     /locus_tag="IW819_001700"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1244804.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(9520..10494)
                     /locus_tag="IW819_001701"
     CDS             complement(9520..10494)
                     /locus_tag="IW819_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1244805.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         3450 a         2202 c         1806 g         3227 t
ORIGIN      
        1 aaaattgcaa attttgctgt tgttccacct aaatcaatcc caataatctt cttgtccatt
       61 acaaatttcc tttctttcgg ctttcttcaa ttcggtgctc tctttttaat atgcgccacg
      121 ctgtcatgta tgtatcatga tcaatcaaac ggctatcata cagattacgc aattcgatag
      181 tcattaattc aatgtcatag atgcgtgcac ccacatagat aaagatgcca aattttttga
      241 acagctgctg tacatcatat aagctctcca tctttaagca cttccttcat attatacaag
      301 taaaccatat ttttttaaac ccagtaaaat acatattaca aaaagaaata caaaaactag
      361 tgtagaaaga atcctttggt gtatcgaata gcttgtctgt tgtttcggta gacccaaaac
      421 attccctaac actaatccgc caattagccc accgacatgt ccccaaacat ctacagaggt
      481 atccagaaga ccaaaaacaa atgtcaacac aataaataaa agaaaatgtc tgactagctg
      541 ctggatcgct gggttatgtt taaaatgaaa ccctaagata aacaacgcac cgaacaaacc
      601 gaatatcgaa gtacttgcac caccagacag aacattcgct tcgttaaaag caaagcttgc
      661 tgtatttccc attatcccgc tgaacatata aatcaagaaa aaacgccaat ggccatagat
      721 tgcctcgatt tgctgcccca taaaatacag tatcagtgag ttcattacga aatgcatcag
      781 tccaaaatgg atgaagattg gtgtcactaa acgccaccat tcattgaagt gaacaagata
      841 aggaccatac attccggcat aataagggat ctcaagtcgc ggaaataatt ccatcatgag
      901 gaagaccgct gtctgtatcc ctaaaaaagc ataggtccat aacggtcgtc ttaaccatgc
      961 acgcaatttt atttgctgtt gataattcat gaaaacctcc catttcattt tagtcaataa
     1021 acaattgttg gatcttctga tcaaagctct ccggttgcca ttgttcattc aattgttcac
     1081 gaaaaacaag gctacatgtt tttttatgaa aattatttaa ataccggtcg taataaccac
     1141 cgccaaaacc gattcgataa ccggctctat tgtagataat acctggtaca atgaggagat
     1201 cgatcatctc cggcaaatag agcttctctg attgtggctc aagaacacca aaactcgaac
     1261 gtacaagaac ggactgttca tccatttcat aaaacgccat ctgacgtttc ggcaaagttt
     1321 taggtacaac tattttcttt tgttcttttt ctgctcgttg gataaccggc tgtgtatcca
     1381 gttcaaattc attcgataaa gtcgtcccta tcacttttgc tgacttccag tactcgctgg
     1441 cgaatagctg tttatagatt cgctgttctt tttccttttt cttgccactt ttggccagat
     1501 ttttcagctt ttcgattcct ttccggcgta atcttacttt caattggtcc aaaaactcac
     1561 ccttccttct ctcataaaat ggtataggaa aaaaagaaaa atagcaatcg aactttcaaa
     1621 taaatgcata aatagaataa aaagaagacc tagaaatgat tggccttcct tttttaatga
     1681 atttcaactg ttcagcgcgt gtttttgtct gataaaaagt gcatacagaa aatatggcgc
     1741 accaaaaagg gcaatcaaaa ttcctgtagg tacttcccca cttagtagaa tcactctagc
     1801 aaccatatct gcactaagca gaaaaatgga accgaacaaa ccgctcagaa caaaggtcca
     1861 gttattttct ttacgtacga ctagcttcgc agcatgagga gctagtagtc ctacaaaaga
     1921 taaactacct gtcaccgcta cactaccgct tgccaaagca acagctaaca acaaaaggat
     1981 tttttgactt ttagccacgt taaggcctaa tccttgcgcg cgttcttgac caaatgatag
     2041 caatacaatc ctctgtgcat aaagtcctcc tagcggaatt aagataaccg cccatggaag
     2101 caatgcccaa acataaggcc agctcgcacc ccagattgat cccgctaacc atgcattcac
     2161 aaatccataa ttttccttag ataattggat cgtgcatagc aatgttaatg cagaaaggcc
     2221 ggcattaacg gcaataccgg acaataagat tcgattcata cccaaaaagc catttcgatc
     2281 ataggccgca agatagacac agcaagcagc taaaacacca cctagaaagg ctacaactag
     2341 caatagaaac ggcagcgaat agtgcgaaaa gaaacctaaa taaagcatca ctaaaaatcc
     2401 tgctccggca ttaatgccca atatactaga atctgccaat tcatttcttg ttactccttg
     2461 caagatataa ccagaaacgc caagacaagc tcctgctaat aaactgatca gtaatcgagg
     2521 aactcgaaat tctctaacaa tcagcgccaa agaagatgat tctttcccta tgaacgtggc
     2581 aaaaagctga tccaggtaaa taaaaggcgt accaatcatt aaactaccta tagacaggat
     2641 gacaagtaca aaaagcaaga aatagaacgt ccaatatatt tttttcaccg ttgttccctc
     2701 cttacttgta tcagtaaaaa cggaacgcca atcaatgcaa tgactaatcc aaaaggtgtt
     2761 tcaaaagggg gattgatcat cctcgctgta aaatccgcca acataaccaa tccaccgcca
     2821 actaaaaaag agacaggtag aacataacga tagtccgttc caaccattcc gcgaacaact
     2881 ggcggaacca acagcccaat aaatgaaaca ggtccgacta acgaaacggc aatggctgct
     2941 aacactgcaa ctagacagaa aacaatcaac cgcgttcgtt taggatgact tccaagagct
     3001 aatgcatgtg tttcactcaa atgaatcaaa gatattgctt ttcctaatga aaaagaaacc
     3061 aaaacagcaa gtatatataa aggaaacaat actgctaatt gtttccatgt gatattggca
     3121 gctccgccta caaaccaaaa agcaagatcc tgctgcagat taaagaattg ggtaagtgct
     3181 tggctgatag cagtaaaaaa agaactaata gctacgccag ctaaaatcag ctgtaaagga
     3241 ttcatgccca ttttcgaaaa ctgcaaaaaa ccaaatacga gcaaagttac agcaaaagaa
     3301 cctgccaatg acattagaaa cacagaaaaa tcagaagctt taggccaaaa agcaaaacta
     3361 agggccattg ccaacgaagc acccgcgttg attcctagta agcctgaatc tgctaaagga
     3421 tttcttgtca ctccttgcat caatgcaccg ctcaatgcaa aacaactccc tacaagaaat
     3481 gctcccgcca cacgaggcaa acgaatattt cgtatgattt ggtgactttg ttttgatcta
     3541 tcaaaatcaa acaacgcctg ccaaaaatct gcttttccca cagtcgctgc gccatttgaa
     3601 agagcaacaa aaaaaataat caaaagaaca aaacagacca aaagcaggag tctatttttc
     3661 ttcatagtcc tcataattga tcctcctgct cttttggcaa caaatcatag gttagaaaaa
     3721 ctggtttatt tgttcttgga agcgtgacaa tcgttgcatc aatctcaaat atttttttca
     3781 tacactcttt tgtgatcact tcttcaggtg taccttctgt gatcaactta ccgtttttca
     3841 ttgctaataa ataatctgaa aaacgtgaag catgattaat gtcatgaatc gacatcaaga
     3901 tcgttgtttg tttcatttga ttgatttcag tcaataactg taggatctct aattggtgtg
     3961 ccggatctaa aaatgttgtc ggttcatcca agatcaagat atctgtttcc tgagcaagcg
     4021 ccatagcgat ccagacacgc tgggtttgtc caccagacag cgttgcgata tcccgcatac
     4081 gaaatccagc tagacctgta gcatccaacg cccactcgat tgcttcatga tcttgcgtgt
     4141 ccatgccact gaacagtttt tgataagggt atcgtccgta agatactact tcttctactg
     4201 tcatacctgt agggtgttca ctagtttgtg ctaataatgc aatttttttt gcaagttctt
     4261 ttgaactgta tgtagagata tcagagttat tgacaatgat ctttcctgat ttcacaggta
     4321 gtatacgggt aaaacttttc aataaagtag attttccact tccatttggc ccaatcaaac
     4381 tagtgatttt cccttcagga atcgtaacag acatgttttc aatgattttt ttgttttcgt
     4441 agccggctgc aatgtcagct gctcttaatc tcgtcatttc acccatcctt tcgctgaaaa
     4501 tcattctcaa ttatgacgct gtttcaacat ttcgtcaagc gctactttca tatctatagc
     4561 caaatgaagc ggttgacagc gttcgaaata tcaggaataa taaccaatga gaaagattct
     4621 caattatgtt aggaggaaag aaatgaaatt aaaaacaaaa gtattcacat tatctagcat
     4681 attgctgctg gctggagtac tgagcgcttg ctccccctct tcagataaag aaacagctac
     4741 aactgccaat tcgacgactt catccaatag tactcataca gtcacagata cacttggaca
     4801 taaagtagag attccaaacg cgcccaaacg gatcatcggc agttacttag aagactatct
     4861 gattgcacta ggggaaaaac ctatcgccca gtggactgtt ggcagtggtt ccattcaaga
     4921 ttatctgcaa gatgatttaa aggatgtccc aacgatttct tatgaccttc cttatgagaa
     4981 agtgttaagc tttgagcctg atttgctgtt gatttcttct tccgctactg tcgaaggcgg
     5041 gaaatacgaa caatatagca aaatcgcgcc aacttatgtt gtaaaaaacg gaccagatgt
     5101 cacatgggaa gaacaactaa aagatgtcgg caaagctttg gcaaaagaaa aagaggcaga
     5161 aaagatcatt tccgattatc aaaaatccat caagaaaact cgagaagaac tagcagataa
     5221 aatcgagaac aagacagctg cagtgttgtg ggtgaccaac aactcagcct ttatggtcag
     5281 caaaaatcgg tcaagtggaa gaatcgtata cgacgatctg gaatttggtg ttcctgatct
     5341 agtggaagag gtctccaaaa aagccacttc tgactggtct gctgtttcct ctgaaaagct
     5401 agctgaatta gatgctgact acatcatcct tgtgaacagt gacaaagatg ctgccatgtt
     5461 caatgaacca agctggaaga atcttaaagc agtgacagac aatcatgtac tgcaattcgg
     5521 acctgaatct agttggctat ataacggacc tattgccagt caggaaatgg tcaatgatat
     5581 caaagaaaaa ctaaattgat tttaactaaa aaaagcatct tcacgattag tcgtgatatg
     5641 ctttttttct tattaacaaa ctagacgcat agattttatt cttcttttga agcaacccta
     5701 ttttttactc tttcccattt ttgaaaacga tgggcatata gattttcttg atccgttatc
     5761 ccctcatact cctctataag ggcgaagtct gaaaaatcaa tattcccaat atacgtatcg
     5821 ccttcaaacg tgctatcgat caatgttcgc cagatccgac cagtgtaagg aagaagttct
     5881 tgaaaaatcc gactaccacc cgaaacatag atatcttctg agatcgtttg actatacgac
     5941 aaaagttcat cgatcgaatg cattacttcc gcattttctt tcgtgcgaaa atttggctgc
     6001 gttgtcaaca cgatgatatg gcgataagga agtgaaagat cccccattcc ttcataggtc
     6061 tttcttccca tcactaaaat tcgatccatt gtatgttcgc gaaagaaacg catatcattt
     6121 ggcaatcgcc atggtaacaa accatttttg ccgatcagac catttttatc ttgtgcccac
     6181 atggaaataa acatattatc cgctccttat gattttagat gttttgagat aaaaaaagtc
     6241 tcaattcttt gaccagcaag gaattgagaa ctttaaaaac attattttgt ttcacggtgc
     6301 aaagtaactt tgcgttcgcg tgggcaatat ttttgttttt ccaaacgatc aggattgtta
     6361 cgtttgtttt tgcttgttag atagttacgt tctttacaag aagtacactc taaagtgatg
     6421 tttacgcgca tgtttttccc tcccattttt acaagaatcc tctaagatga ctctcagact
     6481 tgattatctt accatgattt tttctagatt gctagtgttt ttcacgattt tgttaagtaa
     6541 aactacttga cgataaatac aacggtttta cttctcaaac aaaatctttt tctttttgta
     6601 taaataccct tacacttgtt tatatagatt atgtttttta ataaaggggg atactaatat
     6661 gccatggaac atgaaggact ttcctgcatc aatgaaaaac ttagataaat tgacaagaaa
     6721 aaaagcaatc gacatcgcaa atgcattatt agatgaaggg taccctgata accgtgcgat
     6781 cccaattgcc attgatcaag caaaaaaatg gtatgaagat gctagtgaat cagaaaaacg
     6841 aactttcgaa aaagaaaaaa atccaagcaa gacggatgaa cacgatacga atcctcgtgc
     6901 tggaaaactg cttgattcag atgtgatcgt agaatatgag gaagaacaat ggatcgtgaa
     6961 atctaaaggg gcaaaaaagg caagcaacca ttttgataca aaaaaagaag caatcgaaaa
     7021 aggaaagcaa gtagcacaaa acaaagaatc aactttgttc atttataaaa aagatgggac
     7081 aaaagaaaaa gaaatcgatt attgaacata tgtaaagggg ttgtgacgaa aatcgtgtca
     7141 caaccccctt atgttcgaaa caatggtgtt cctctgccaa ctccgaacca taagtcataa
     7201 tatctataaa acgggaagag ctgttttttg aaattgtttc ttatcactca gtgttaaacg
     7261 gtaggagaaa acctcttttt tattgatatt tgattttcat caaacagtca catagtcgat
     7321 tgcttctgct tgcggaatcg tttcatctac aaagtagact ttataccatg tcaagaaact
     7381 gaagattgtc cagatttttc tttgctcttc tttacgtcct tcgtgatggt catctagcaa
     7441 cttcatgatt ttttcttgat caaaaaattc ttttgcaaaa tctgcttcga acaatgcttt
     7501 gacttgttcg tagccgtgtt cttctttcaa ccaagctttg attggcacag ggaatcctaa
     7561 tttcacacga ttcgaccatt tttccggaag atgtttgttc gcagcttgtc tgaaaatatc
     7621 ttttgtgttg ttttgattca ataaaaactt acttggtatt tgttgagcca atttcatgac
     7681 ttcgatatcc aataaaggta ctcggacttc caaagaactt gccatagaaa gtttatctgc
     7741 ctttaacaag atatcttttg gcatccattg atgaagatcg acatattgca tcttattgac
     7801 ttcatctttg attccttctg ttttctcgta atgaacagtc atgatttcat ccacagatgg
     7861 tgctgtttga tattttggtg ttaacacttc tgatgcttct gattcttcga acactcgagc
     7921 atgaccgata aaatattctt ttgcaggagc aagtgattca tataaatgga tccgtccatg
     7981 gaaatttttc atttttccga ttttacgtcc taaattatag cgtgttcctt ttggtaattt
     8041 tttcaatcct tgagcaatca cacggatgaa ttttgaattc gtatggaatc catacgtttg
     8101 gtatcccgca aacagttcat ccgccccttc tccagattgg actacccgca cactttgtga
     8161 agcaagattt gctaaaaagt acaaaggtac gcaagaagga tttgaatcag gttcatctaa
     8221 gtgatactgg atcaaaggga atgctttgaa cgacatgtct ccgtcgatca ctgctgctgt
     8281 attatctaag tccaatagtt cggttagttt tctggcttca atcgcttcat tataggtttt
     8341 atcatcaaac ccgattgaga aagaatgatc tggtttcaat acagaggtta cataactgga
     8401 atcaaccccg ctggataaga aagaaccaac ttctacatca ctgaccgtat gtgcttcaat
     8461 agaagcttga acggtttcgt cgattttttc gatccattct tgtcgggaat gcgtttcttt
     8521 tgtttcaaaa tctgcatccc aatattgctg aatgtctatt tttccatctt tatacgtata
     8581 ataatggcct tcaggcaaac gatagacatc tttgaagaat gtttcgccgt ttaatggaga
     8641 atattggaaa gtcatataag gtttcaacgc ttctttgttc aattctttgt taaaatcagg
     8701 atgaggcaga aaacttttga tttctgaacc aaacataaat gttccgttca tttctgcata
     8761 ataatatggt ttgatcccaa agtggtctct tgcaccgaat aactcattct tttcattatc
     8821 ccaaatagca aaagcaaaca ttccgcggat cttttgcaat aattctgttc cccattcttc
     8881 gtaaccatgc aaaaggactt ctgtatctgc atgtgtttga aaaacgtggc cagctgcaat
     8941 caattcttct ctcaaaggct ggtaattata gatttcgcca ttaaaaataa tgatctttgt
     9001 ccgatcttca ttgtaaatag gctgagtacc gccctctaag tcaataatac ttaatcttct
     9061 gaatcctaaa gcaacatgtt tatcgatata ttctcctgaa ctattaggtc cacggtgaac
     9121 gattcgatcc atcattgtat taataattgt ttttttgttg tccttgtcgt taacaaaacc
     9181 tacaataccg cacatttaac tttcacatcc taatataaaa ttactgttta tttcattcat
     9241 atgcatctta tcatagaata aatagcggac gaatattttc tctcttacaa tttcgtcacg
     9301 tgaaaacaaa agatgaaagt ttctgttttt acagaaaata aagtcccaat atctaattta
     9361 ccatgttttc ttttttgagg ctaccttatt ctcttcgtgt taaaaaaata ttagtggatg
     9421 ctatttccaa aaaaaccagc aaaaaaagac acgacctaaa gatcgtgtct aattatgact
     9481 gtatcagaaa atatgatacc aaacggatct attcattaat taattgacat agtttatctg
     9541 ttgctctttt atgactggat aactgaccaa ttcatgacga tccaagtctt ctttattcat
     9601 atttacagca gtaatttgac tgtcttcata cgtacgataa taataaatcc ctttatcaac
     9661 gttacagcaa gaagaataaa tcgtatattc gtattttctt tctccagtat cgcatactcc
     9721 tttttgttgc tcgactgagc ctagtatatg aaaaaattga ctgatacttt cagattctga
     9781 atcacctgat gcagcattca ttttagtaaa tgtggctttt acaaatcgag aaacagaaga
     9841 gagatctcca ggtaagccca atcctcccat cccacgacta taaacattca agctgatctg
     9901 gtttgaaaaa tgattttctg gtgttccatt tgatagcgag cgataattgt tcagattaaa
     9961 taattgataa tcaaatggcg gattatttgt caacacaccg acaggattat catataaatg
    10021 aagtccatct ttcatacttt caattactat cgatttttct ttatcagcta ataaccagtg
    10081 taaaggtgac aagggtagtt tttcactaaa atcgatattg actagattga tagtagccaa
    10141 taattcttta gcttcgtcta tggttgagca ttgccctagt atccatggaa taaactcaaa
    10201 tggtgcaacg ttatcttttc cttcttgtaa ttctttataa tcagcattgc caggaaaatt
    10261 cagtcctgcc atactcaacc ctttttcatt tgtagcctca taatagagtg gatagttgga
    10321 taccccagct gctattccta tcattgcata atgtttatct aaatttttta cctttctaaa
    10381 atcgaaacga taatttcttg gagtaatagt gaccacttca ttataagaca tctcataatc
    10441 aaaattcctt ccaaaataat ggtctttcgt tgcataagtg attgcggtac acatcgtttc
    10501 ccgtcctaaa acaaatcgtt tttacacttt tatcttacat ctagtatttt ttttatcaaa
    10561 aaaaggccaa agaagattga tttactagca tttataaaaa atagtactca gggggaatca
    10621 aaaaatattg taggttaaaa gtacaacaat aaggggctgt gacaaaagtc ttgtcacagc
    10681 ccctt
//
LOCUS       DACEPW010000085        10578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEPW010000085 DACEPW010000000
VERSION     DACEPW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.086"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(127..1458)
                     /locus_tag="IW819_001702"
     CDS             complement(127..1458)
                     /locus_tag="IW819_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1244806.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENTRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(1613..2014)
                     /locus_tag="IW819_001703"
     CDS             complement(1613..2014)
                     /locus_tag="IW819_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1244807.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(2100..2306)
                     /locus_tag="IW819_001704"
     CDS             complement(2100..2306)
                     /locus_tag="IW819_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244808.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(2401..3903)
                     /locus_tag="IW819_001705"
     CDS             complement(2401..3903)
                     /locus_tag="IW819_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1244809.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPKNEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(3921..4376)
                     /gene="argR"
                     /locus_tag="IW819_001706"
     CDS             complement(3921..4376)
                     /gene="argR"
                     /locus_tag="IW819_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1244810.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(4474..4881)
                     /locus_tag="IW819_001707"
                     /pseudo
     CDS             complement(4474..4881)
                     /locus_tag="IW819_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4896..5090)
                     /locus_tag="IW819_001708"
     CDS             complement(4896..5090)
                     /locus_tag="IW819_001708"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244811.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKIG"
     gene            5311..6684
                     /locus_tag="IW819_001709"
     CDS             5311..6684
                     /locus_tag="IW819_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1244812.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEKLQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            6759..7871
                     /locus_tag="IW819_001710"
     CDS             6759..7871
                     /locus_tag="IW819_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1244813.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTSHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(7943..9580)
                     /locus_tag="IW819_001711"
     CDS             complement(7943..9580)
                     /locus_tag="IW819_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1244814.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILKTYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(9748..10356)
                     /locus_tag="IW819_001712"
     CDS             complement(9748..10356)
                     /locus_tag="IW819_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1244815.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         2782 a         2201 c         1940 g         3655 t
ORIGIN      
        1 tttttctaag atatcttttg tcaaaccaaa gccaccgcct gctggtttca agaactcgac
       61 ttgcgggtca gttagaacta ctgctgtatt tttgttgttg aacatgataa attctccatt
      121 cgttatttat tttaatacat catttagagc ttcactcatt gttggatgag aatagatttg
      181 atctctcaat aacgtataag gaagttttcc attcatagct agcgaaatca agttgatgac
      241 ttcatgagaa tcttccgcat agatacttgc ccctagaatc aagtcagttt cagggtcgac
      301 taaaactttg aatactcctt taggatcttc taatacttgt gctttaggaa caccgcttgt
      361 catatacttg aataatttgt aattgacgcc ttgtgctttg gcttgttttt ctgttaatcc
      421 tacattggat aaagttggag tgataaatac actgtaaggc acagctttcc ggttattcgt
      481 tgttcgtgta ttctcgcctt tcaactgatc catgataatt cgataatcat ctagagaaat
      541 ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc caaatgttgt ctgcatctgt
      601 acgaaggtat tcatccacag cgattgctcc gcgatctgtt agtttaacat ctgtattttc
      661 taatccaagt gatgctgtac ttggttttct tccagttgca gccaaaactt ttgacgcttg
      721 gattgttact tcttggccat cttctgtaaa ggtgacggca gctgttgatt cttggtctgc
      781 tacttgatct acagatacac ctagatggaa ttcgattccc gcatttgtca tgtcttctaa
      841 aatcatttgc gcgatatctt catcttcacg aggaagaaat tcaggatggg catcaagaac
      901 gacaacttta gatccgtatt cattgaacat tgatgcaaat tccaagccaa tatatccagc
      961 cccgatgatc actaattttt ccggtaattg ttcctgatcc atcgcttgcg tagaatcaat
     1021 caagtagcga cttgtattca atcctttgat tggtaaaatg attggttcag aaccagtatt
     1081 gatgaaaatc cgttcgcctt caacaatcac tttttcacca ttattcgtca cttcgatttg
     1141 atggttagaa agaaaacgtg ctgtaccgtc catcactgtc ccagttgcct catcgctgat
     1201 catatgataa tttttggctc gaagcatgcc cgtcaatttt tctttccgag caacagcctc
     1261 tgtaaagtcg attcctcttt ctccattgaa aatcaatgat ttagaaggaa tacagccgat
     1321 gttgatacat gttccgccat acatacgtgt tgacttttca acgactaaaa ccgattcgcc
     1381 ttttgaagct aacgcctttg ctaaagtctt accgcccttt ccaaaaccga tcacaatatt
     1441 ttgatatttt tccatcattt ttccctcgct tcttttttac ctaccgattg gtacaaaaat
     1501 atgataaccg ataatcgtct attttcccaa tcatctgctt tatttgtcaa aaaaaaccag
     1561 acaaaaagcg gtcctttcac gtttttagtc tggttcgatt tcctttattc ttctaaacaa
     1621 tcgttacttc tacatcgata tttcctctag ttgctttgga gtaagggcag aattcatgcg
     1681 catcttctgc aattttttgg gcttcttctt cgctgatgcc atcgatatgc acttctaaca
     1741 ctacaccgat ctgatagccg ccatcatcta aattataaag agagacacgt gcttttactg
     1801 tggactctgc tgtcattcct gcttctttga tcatatgttg taccgcccca ttgaaacaag
     1861 aagcataacc tgctgcaaat aattgttctg gatttgttcc ttctgcatgg attcctggag
     1921 gagtgatttt catatgcatc gagccatttg gtgcctcgac ttctccattg cgcccgccat
     1981 ggttgatcat tgctgtttcg tatgcttttt tcattgtact gctcctttcg attgaccatt
     2041 tgctacttgg ctataattat agtgtatgac aagtaaggcg ctttctacaa aaattctgct
     2101 tattcgaata ccagaatgcc gtcttctact atttttccaa aaaacaaaaa aatactactc
     2161 gtgaacaaca gcgtcatatt tgttccaaaa taaattgcaa tcttttctac ataatctttt
     2221 atatgattga ttttaagata catatatacc agaaagttga ccccataaac caatactgtt
     2281 aaaaaaataa ccatattcgt taccattttt tcctcctttt gctggtctcc ctaccagcat
     2341 tcgatttaac cataccactt tttttgccgt ccaagcaact agcctttcaa aaagactggt
     2401 ttattctgct gttaatgatt caagatcaga aaaatgtccg aataattttg ctaattttac
     2461 taacgccaac ttgttctttc cgaattcatc attttctatc tgtaatacta gtaacggctc
     2521 atgtaagctt aaacggatca aaaaccagcc gtttccgtat ggctcttcta gatcataccg
     2581 aataccttct tcatttttcg ggtcgaagtt catccctttt tcttcttcca acagtcgctt
     2641 catttcttct aatatcttgt ttccttgttc gcgtggttca tcgctgaaca attttagtcg
     2701 gatttcttgt acttctacgg gctgtttcaa ctctttgatt tgttcatcta atgtgatacc
     2761 ttttcttttt aattctggca aaagcatcag gatctttgcg atgacatatg caccatcatc
     2821 cagaaaataa ttttccttga atgcagcgtg tccgcttgtt tcgatggcta gctgtgtatc
     2881 tttcccaaga cggttaagtt ctaatgcttt attgattaca tttcgatagc cggaaatata
     2941 acgaacttgg atgcctcctt tttcttcaat gaatctttgt agatgagaag aagtagggga
     3001 attcgtcacg attcttgttc ctggatgctc ttttaatacg attgtactta atactgcgat
     3061 caaattattt cgattgatca ctgttccaga agcactgaca actgcagaac gatccacatc
     3121 tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa acagcctgct tgatactttc
     3181 catcgcttct ttattatcag gatttgggat atgattagga aatcggccat ctggttccaa
     3241 aaattgggaa cctgttgaat ccgcacctaa gctattcaat actttttcag caaagaatcc
     3301 accagcacca tttccggcat caacaatgat tttccaacca tttagtggtt tgctttctag
     3361 tgttccaatc ccctttttaa tttttgtgac taaatcttgc gcataaggtg tcaaaaggtc
     3421 tgctttttct acgcttccag tttgttcgct ttttattttt tgctgctgtg ataggataaa
     3481 agcaatatct tctttttctg ctcctccttc tgaggtaaag aattttattc cattataata
     3541 atagggaaga tggctagcag tcagcataac cgcagcatcg cacgagaact gttcaaattg
     3601 agtggacata aacatagcag gcgtagtagc taattcgaaa tcgatgacat gtatcccttg
     3661 ttctttcaga ctttggatca acgcctcttt cagtgctggt ccgcttatcc tactgtcatg
     3721 accaatccca atcgtccatt ttttatttgg atcggttgat ttcttctttt tctttatcca
     3781 gtttgctagt ccccagccga tcttttttac catgtctgtc gtcaagttta cttcatatcc
     3841 ttcaactggt aatgcgattc ctcgaatatc cgatccattt tgcagcgctt tcatatcttt
     3901 catataaaag tcctcctcat ttattccctc attttttcca gcttttctgc aaatcgctgt
     3961 gcttcttctt ctgagcggca aataataatg aatgtatcta caccggccat cgttccgaca
     4021 acttcagggt aagccacttc atctagccaa ttcgttacca catcggcacc atttcgttca
     4081 gtcaatacga tgaccatgaa ctgaacagac tggatacgca aaacctcgcg tctaattgct
     4141 gtacgcaaac gttcttcatt taaagaaaca gatggctggt taaataatgt atagcgtact
     4201 attttgtttt cattttgact ttttactaac ttcatttctc gaatgtctcg agaaatcgtt
     4261 gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa gcagctcttc ctgtgtccca
     4321 ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc gctgtttctt tttcatatta
     4381 gacacctctc aatgttgatt atagcaaaag ataagtaata tttcttcata ttttggtatt
     4441 ttttactttt attttttgtt ttcattcagg ttgctaacct ttttctattt taacacgcaa
     4501 aatcgtcttc attttttcag gtgctgattt tcttggatcg ctgatgtaaa tctctttgtg
     4561 ttctttagaa gctctttcat agccttcttt tgcgatgaat gcttctaatt tctcaaacgt
     4621 tgctggttct tcgtcatagg gcccgacgtg taagatttgg gcacaaagac cttcttccat
     4681 ctttttgaat actacttgtt ctttcaagtc ttccggaagt ttgctagaac gctgaatggc
     4741 ttcttgtgct atcgcttctg taacaaagtc aggctgtcag atcatcaatt gataagaaaa
     4801 atgttctttt ttcatcactg gttcatgcaa atacttttct tgcagtcccc attgtccttc
     4861 cagtggatag acagtataca tctgataatc aggaatcagc caattttttt aggcgacatg
     4921 cgtattgcat agctgactgc atatagacag gctactcttc gtttaaagtc ttcctgattc
     4981 gggtcgccga tccctctgat gctaaagaat ggttttttag gaacctctag tatttgcggg
     5041 ctttttttca tactatagct cttgtcttta cgccattcaa ttttttccat ttttcttcct
     5101 ccgcgaaatt ccatttagtt atttattatc atacctcttt ttgagtgact tggctaatcg
     5161 tgttgacact atttttcagt ttgtgctaga ataagtgaag taaataaatc gtacagataa
     5221 cacgtttcgg cgtgtcgaga gagaggcggt ttctattttt ataatagaaa tcactctctt
     5281 tttttacgaa attttttaga ggtgatgtaa atgacaaaac aaaaacagct gcttttagta
     5341 cctgcagtaa ttgcaacagg gatgatgtct tttgcgggcg tattgatcga gacggccatg
     5401 aatgtaactt tcccaactct tatcagacag tttgatttaa cgactgcaca agtccaatgg
     5461 gtaacaacga tttatctttt gatgatttcg atcattgttc cgctgtctac ttatttgaat
     5521 cgcaatttta gtctcaaaaa actcttttta gcttcaaatc tgcttttctt ggcaggtata
     5581 atgatcgatt tcttctctcc taccttcatg gtcctattgt ttggtcgatt gctccaaggt
     5641 gcagcgacag gtatcgctct tccgttgatg ttccatatta tcttgacatt tgcccccctc
     5701 aatcgacgag gcgcgatgat gggtgtcggt actttgacaa cggccattgc tccagcaatt
     5761 ggtcccactt atggaggcat tatgacttca cattttacat ggaatcatat cttcctgttt
     5821 cttgtgcccg ttttgattct ttctttgatt atcggctggt ttgctattcc cgagatggca
     5881 gtacaaaaaa gtggttattt ggattggata agcgttttag gaatttgtct tttgtttagt
     5941 ggaagtctaa catttttgag tacgttggga acagtttcca gctggattgc tttaatcatc
     6001 ggcggtattg gctggttcct tttttactat cgaagtaaaa aagtggagca tccgctgatc
     6061 cgactgtcta ttctttcaaa taaaacattc cgcttgtttt tgattggctt cttagtttat
     6121 cagtttttat tacttggtgt atcttttgtc ttgccgaatt tccttcagat cgttcaagga
     6181 agtagtcctt ttgtcgctgg attagcgatg cttcccggcg cagcggttgg tgctttgctt
     6241 tctccttttt ccgggaaaat gctggatcaa tatggtccga agaagccaat ttttgctggg
     6301 ctgattcttt ctttgattgg ttggctagca ttattcctac tcataggaca cgcttcagtg
     6361 ggtgtattga ttgcttgtca tgtgttttat atgatcggta tcggattatc ttacagcaat
     6421 atgatgacca ccggaatgaa tgcactgaat gaaaaactac aaggagacgg gaacgcaatg
     6481 ttcaatacct tgcagcagtt ttctggcgct gtggctactt cattagtagc ggtcattatc
     6541 aattacgtcc agcatcatac ttcccataac tatgaagtgt ctactacatt aggatcaaaa
     6601 gcagcattag gcgtcttgtt gctgttgctt ctcgtaagct ttgctcgatt cgcctattat
     6661 ctgttctttg ctaaaaaagc gtagattcca tatacggtag gtgttgcatc tgccgttttt
     6721 ttgtgtgaaa atggttgttt gtaaaaggag gcttttttat ggaaaaaatt gccattatcg
     6781 gtggcgggat cattggtatg accctagcca gttacttaga tacatcaaaa ttcaatgttg
     6841 ctttatttga tgaagggaca ggacaggcaa ctaaagctag cgctgggatt atctcccctt
     6901 ggttgtcgaa acgcagaaac aaacaatggt atcgtctggc taaagacggc gctgcttttt
     6961 tccctaaact tataaaagat ttccaacttg atcaaacgat ttacgcacaa tcagggacac
     7021 ttttgcttcg acctgaaaaa gatttagctg aattggctgt tcttgccgag aaacgaaaag
     7081 aagaagcgcc agaaattgga aacatcgaat ggctttcacc tgaacaaaca tctcaagcac
     7141 ttccgttatt gaaaccagca gtttcactgc gtatcagtgg tggtggtcgt ctggatggaa
     7201 aagcatattt agaacatctt ggtcagcgtt tagcacaaaa acaaatggag cagatccagg
     7261 caaaagtcac ttcgcataag aagagcgatt cttggctcat caagggaccg tttggagaat
     7321 acacagctga tcgtgtaatt ttgacacctg gcccggcact taagccttta ttacaagaat
     7381 taggctatgc tgcagatata cgtccgcaaa aagggcagct cttagcattt cagacatctt
     7441 ataagatgag tcaagactgg cctgttgcta tgcttgacgg agaagctgat ttcattcctt
     7501 tcatggatgg aaagatcctt ttaggtgcta cccatgaaaa cgatcaaggc tgggatttgg
     7561 ctcctactag cgaagcttat aagggattga ctaagagtac agaacagttc ttgactgatc
     7621 cagatcagat aaaaaactgg ccatacgagt tccgtgtagg tacccgtgct tatacgtcag
     7681 atttttcccc atttttcggc gtactgccag atgatccaag cttactggct gctagcggtc
     7741 taggctcttc tggcttgacc acaggtcctt atatcggcta tctattagcc aacttcttaa
     7801 atactggtca ttggcctacg gaagattatc aaaaggacat ctccacctat attacgaaaa
     7861 atccatcata gaaaaacagc tttttcaagt aaggatcccg agcgatattg cgcatggatt
     7921 acttcttgaa aaagctgtta ttttagttca atttctgaat caaattctcc aaactttctt
     7981 ttgcttcatc tggcaagaga tcttttccta tctgagcttc aatgaatcgt aaacgaatat
     8041 caagtcgttg tttcaccaga tttttatagg acgtatcctc aaaagaaaca ggaaaatctg
     8101 gtataggtaa atagtggatg tcttcaaatg gaccaaacga cttgaattct gccgtttgtt
     8161 gcacgattct ttcaacgatt gacaatgtcg tttcaggttt gattttttta tcatcgaaat
     8221 atccagtatt caggacatag caggctgttt gctgtttcaa aaacaactct cgaaaagctt
     8281 gatagtcttc cctaagagga tagacacgga aaggattagc aaatggttca atgactagct
     8341 catctagctg tctttttgta gtgatatttt ccgcgtctga tcgttttgtt gctaatgttg
     8401 caccaaaaga cgctgctaaa accgggtcat tgatcttgat aattggcgga agcgaatcat
     8461 ctttcatgat ccaaaaaaca gcatcgatct tttcgctaag atgatcgact cggtcgggtg
     8521 tcgcataacg tgatttgact gtccgcccat ttccgtttcg tatatctcct gttactagga
     8581 ctttttctcc tttacttgta catgtcacgc ccacattctg acaagtaagg aaagaagaaa
     8641 tggccggatc atcagacgga tagtcttgtg ttttatcaaa gtaagaaggt tccaaggcag
     8701 tagcaaaacc attttttcga gaaataacga aagcatcgtc atgcaagatc gaaacagagt
     8761 gaccttccgt atgttttgct aaggtaatcg ttgattttcc agaaccggac aacccaaata
     8821 cagccatcgt ataggtctgg tcattactag catattgttt catcccaccg tggcaggcaa
     8881 taaaaccatt gcgatttccc aatgcccaag caagcgtcaa agtcgctttt tttaattctc
     8941 caaagtaacg taatcctagc acagccgctg tattatggag agcatcaaac aaaatgagtc
     9001 cattgggaaa tgtttcatgt gtccactcgg gatcacaaaa gatataaaga tctccctcat
     9061 caaaggaaac agacttttga taagtcttta agatcttcga attcgctggc tgaaaattca
     9121 gcaaataaga atacaaattg ttctcaaatc cttctggaat cagcaaatga ctttgcacag
     9181 aaaattcttc agataggcca acgattactt ttgtatggta aaactggcgt ttgcgacttt
     9241 gatacacagc ttctcttaaa attttctcgt atttgctttg ttccttgtct aagtcctcaa
     9301 agatccgccg agcacttgct gttcttccca caatgcttcc atcattgaat actagtgtcc
     9361 tgacatcagc tggtaaatct agttcttcag catgtgagat tgttaaatct gtgatgatcg
     9421 ttcctggact cccttttgct agttgataag ctgtcttcct gtcaaaaact tcatgaacat
     9481 tattaccgta aaatgtcgtt tctacaatcg atcgaatact ggataacagt ggatttccac
     9541 ttgtgatttc gtttaattcg agactctctt ttgtactcat ggctcctcct cattccctaa
     9601 acaaaaaaac gttttcttta ttatacatta ttcgagtttt tatttgtact gctgcgctgt
     9661 gggaagcaaa cgagttattt ttcacacaaa aaaaccgaga acttgaataa acaagtttct
     9721 cggtttcatc attttccttt tatcggctta gtaccagccg tttgctaacc agaatgtttt
     9781 agcagcatcc catgaaccat aacgagatgt tacatattgt tctgctacac gttcttgatt
     9841 ggcagctgaa tagtcaccgt ttaagtatga agcatctaat tgataacggc cgatgtaacg
     9901 tccgttagta gctgtgtatg aaccgcctga ttctttttgt gcgatccatt cttttgcaga
     9961 gcttgaagtt ggcgctgttg cagcagctgg ggcagcagga gctacttcaa ctgtttcaac
    10021 aggtgcaacg tatgtatgtt cttgcgttgc tgtttcagtt gtagatgctg tattttctgt
    10081 tgttttgttt tctgttgttg ctgaagaatc atttgaagtt gggattgtta attgttcacc
    10141 aacataaatg cggttgatgt ttgcgatcga atttttttca gcaattgcat cgatcattga
    10201 gttgtctcct gcaaattttt gtgagatctt agataatgaa tcacctgatt ttactgtata
    10261 aacttcatca gcgtgagcat ttgtagttcc tacaaacatt gcagtagcac cagcagcaaa
    10321 agttgtacct aaaacaattt tcttaaatga attcatatta aactcctttt tttctccgtt
    10381 ttttattctt tcgacgctat taactttatc atttagaaat atttcatagg taaaagaatc
    10441 atgacattta atgacaaaat gacgaattat ttaatatttg taacatttaa acaaatattt
    10501 ttttgataca aaaccataaa aaccttgttg tataagcaaa gtaatcgttt ttctatgttt
    10561 ttttattttt gaatgaaa
//
LOCUS       DACEPW010000086        10547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEPW010000086 DACEPW010000000
VERSION     DACEPW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.087"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..339
                     /gene="tnpB"
                     /locus_tag="IW819_001713"
     CDS             <1..339
                     /gene="tnpB"
                     /locus_tag="IW819_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1244816.1"
                     /translation="IKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKAD
                     WYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTE
                     GLRLHSMGLA"
     gene            complement(689..1531)
                     /locus_tag="IW819_001714"
     CDS             complement(689..1531)
                     /locus_tag="IW819_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1244817.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            1856..2272
                     /locus_tag="IW819_001715"
     CDS             1856..2272
                     /locus_tag="IW819_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1244818.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIRESQQSVIDCRKLLVEQLTGGDDL"
     gene            2269..2739
                     /locus_tag="IW819_001716"
     CDS             2269..2739
                     /locus_tag="IW819_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1244819.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            2753..3526
                     /locus_tag="IW819_001717"
     CDS             2753..3526
                     /locus_tag="IW819_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1244820.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            3540..4352
                     /locus_tag="IW819_001718"
     CDS             3540..4352
                     /locus_tag="IW819_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1244821.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            4406..5371
                     /locus_tag="IW819_001719"
     CDS             4406..5371
                     /locus_tag="IW819_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1244822.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            5487..8210
                     /locus_tag="IW819_001720"
     CDS             5487..8210
                     /locus_tag="IW819_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1244823.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSGEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8223..8855
                     /locus_tag="IW819_001721"
     CDS             8223..8855
                     /locus_tag="IW819_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1244824.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9754..>10547
                     /locus_tag="IW819_001722"
     CDS             9754..>10547
                     /locus_tag="IW819_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1244825.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3533 a         1642 c         2056 g         3316 t
ORIGIN      
        1 atcaaaaatc acgatatcat ctgtattgaa gacttaaaca caaaaggcat gttgcgtaat
       61 cataaactag caaaaagtat ttcggatgtt tcttggtcta gttttgtgtc taaattacaa
      121 tacaaggctg actggtacgg tcggaaaatc atcaaagtag ataagtggtt tccatctagc
      181 caaatctgtt cgaattgtgg acataaagac ggcaagaaat ctctcgaaat tcgagaatgg
      241 acttgcccta tttgtcatac tcatcatgac cgagatatca acgctagtat caatatcctg
      301 accgaaggtc taaggctaca ctctatggga ttggcttaga tcaaataaac aagaaccgta
      361 ggaactacgg ggatagcttg gtaaataaga ggcacctctg ttagtaaagt aatacgctat
      421 caagtatgct ctcttcccaa gaagctccca cttcaagcgt aagaaaccgt taggtttagc
      481 taagtggtga gtagttcact aaggaagtaa acccacacag aaatataggt gattttcctt
      541 gaatgaccaa gatattgaaa gttagtgaaa aaacacaaaa aggtagccct attcacagaa
      601 ggtctgcctt ttgtgttatc gccagcaaac attagagggg atatttcata tagcttttgt
      661 cctctcgaca gtcctatcag tttctctttt aacttatctc taagtgctgt ccagttttaa
      721 tagcttctag cttgtttcct aactgtgttt ttaatatgtt gaaggctttt gaacctgtgc
      781 agtggcaagt aaggattttt tctactcgtg agtcattcaa tttatttcca attttcatta
      841 cctcttgctc aggtagacct aaattattta tataaggtat gccaatcata tggaagccac
      901 cgattaggta ttttatgggt ttagtcggaa atgtttcaag agcaatttcc atcatatcga
      961 taatgccatg atgactacag ccagtaaaga caatcagacc gtcttcttgc tcaattacga
     1021 ccaataattc atctgaaaaa tcatcataaa caaatttatc atccatcttt ttgtataaat
     1081 tcttggtggt cgagtttgct ttgcctaggg ggatgatatg gaagctcgga gcaagttctg
     1141 ttttcgtttt tattgtatgt aaattctcat gcattttaag gatttcattt ttaggaatac
     1201 caacatacag aaacgtggtt aaaatcttta aataatgggg taaagttaca ccttcagctg
     1261 tataaatagg ggcgtttcct gattttaaat atcttaaccc gccggtgtgg tcagaatgac
     1321 cgtgtgaaat aatcactgcc tccagatcat tcaaagtttc ccccatttta gtcgcattat
     1381 agactaattt tttattagcg ccagcatcga agagatattt cttccctgag tagttaatta
     1441 agagagataa cccatgtcca cttttaaagt tcaaatttat tcttgagttt tcaaataaaa
     1501 ccttgatttc cactataaca tctcctttca catgaacgtg tattcatata ttgtagcaca
     1561 ttaagttcta ttttttgttg taattaccga aaaaaatatt aatggacgct ttttacgctt
     1621 tatttcgtat gtggaagaaa atttcttagg atgtggcggg ggattggggc aaagctaaat
     1681 tccaattaca gtgatcattt aattagacac ctttaaatac ccacactttc atcaacacta
     1741 taatttaggg aaagaaaagt gttggcatga tatagaaaaa cctttaattg acagcgtttc
     1801 ctaaaattgg cacgcatctt gcttatagaa aagcgtaagg ataggaggaa tgaaaatggc
     1861 aactataatt cttgccagcc atggggaatt tgctcaagga ttaaagcaaa cagcgacaat
     1921 gattattgga gatagtgtac caattcatgc cttgtcagca tttagagatg aagatgaatc
     1981 tattttggta caaataaaaa aacttttagc cacaatagat atagaagaaa cctacatttt
     2041 aactgatatt ctaggtggta gtgtgaataa tgatatgctc actattatta aagagtatga
     2101 gggattgcaa ttaattactg gaatgaactt acctttagtt atttctattg caacctataa
     2161 tggaaaaatt ggggaaagtg agctagaaaa gcttattaga gagagtcaac aatcagtgat
     2221 tgattgtaga aaattattag tagaacaact tactggagga gatgacctat gattaaatta
     2281 gtacgtattg accatcgttt gttgcacgga caggtagtat tttcttggag taaatcatta
     2341 aatatcaatc gtattattat tgccaataaa gaggcagcga atgatgaatt caaaaaaatg
     2401 tctttgaatt tatctaaacc gactggcatt aaattaacaa ttttttcagt tgaacagtta
     2461 ctagaaaaaa tgcctaaaat cgaaacactt aacgaaaata tcatgcttat ttttggtaat
     2521 acaaaagaaa ttcgtcaatt ttgtgaagcc tatcctaaag tacaagaaat caactatgga
     2581 gggataatca aaaaagaagg ttccaagcaa tttagtaatg ccattttctt gaatgaatct
     2641 gagcaagaag atgccaaaaa attaaaagct cttggcatta aacaatttat tcaacaggta
     2701 ccaacttcta aaaaagaaga tttgaataca atgttataaa ggaggaatga aaatgctcgt
     2761 tcagtcaatt ggtttagggt taatcggtgt tttttgtatt ttagactcaa ggcttttagg
     2821 aagaatgaat tttgaacgac cgttgattgt cagtacgctt gttggtttgg tacttggaga
     2881 catggaaaaa ggattaattg ttggagcctc tttggaattg atgtccttag gattagtaaa
     2941 tatcggagct gctgcaccac ctgatatgaa tatggcatca attattgcta cagcctttgc
     3001 cattttatca aatgcgaacg cagaagcagc attaacgatt gccattccaa tttcagtgtt
     3061 aggtcaaata ctaggtatct taatgcggac gattttgtca aatttgactc acgttgcgga
     3121 tcgctttatc gaacaaggga aatttagcca agcaaggaga attcatatac tttatggctc
     3181 gggattatat gctctcatgt attttattcc tatttttcta gcaatttatt ttggaacgaa
     3241 tcttgtttct cagattgttg atgcgattcc tgagtggcta acaaatggct taacggttgc
     3301 aagtaaaatt ttaccagcat atgggtttgc gttgttatta caaacgatgt taacgaaaaa
     3361 aatggtgcca tttttattac ttggattttt aattactgct tattctggat tgggaatcat
     3421 tggtattgca gccttcgcat gtattgttgc gtttgtcatg gttcaagttc aaggtaaaaa
     3481 ctcaaatggt aaccaaaatg agccagatcc attagatgac ttataaggag gtagattaag
     3541 tgaaagagaa agatcaaaat gcatggaaat atcggcagtt cttatggcgt tcatgggcaa
     3601 ttcaagcctc atggaactat gaacgacaaa tgaacatggg ttttatgtac ggaattgcac
     3661 caattctaga taacatttac aagaatccag aagatgaagc aatgaagaaa gaagcttacg
     3721 agcggcatat gatgtactac aactgtaccc ctcaaacaag tgcatttgtg ttagggttaa
     3781 ctgcttctat ggaggagcaa tactacgaag accaagaaaa ctttaatccg gaatcaatca
     3841 gtgcagtgaa gacttctctg atgggaccgc tatctggtgt tggtgattct ttctttcaag
     3901 gaacgattcg ggtattagcc tttggactag gaattaattt agcccaacaa ggtagtattt
     3961 taggtccaat tctagcaatg ttaatttcct ttgtaccttc ctttttagtt acttactatg
     4021 gtgggaaaat tggctataac atggggaata aatacttgtc taaactgtat caagaaggtt
     4081 tgatggataa agtaatgtat gtttgctcca ttgtggggtt gatggtcatt ggttcgatga
     4141 ttgccagcat gatcgggatt acaacaccaa ttcaaatggg taaagctttt gtcttacaag
     4201 atgtattaga tagtattatg ccacaatttt taccattagg attaaccttc ttcatgtact
     4261 ggttattgca gaaaaaggtc aaaacaggat ggatgttggc aatctgttta gtcggaggaa
     4321 ttatttttag tttgttaggt atcttacaat aagggaataa ccaataaatt tggttgaata
     4381 atataaataa ggaggaattc aattgatgaa ttcaaaagaa attgttaaag aaatcgtagc
     4441 gaaaaaagag gtaaaatcaa tcgtatttgt tggctgtggc gcttcaaaag cggaccttta
     4501 tcctgcaaag tattttctag accaaaatgc gaagcagtta cggattagtc attacacagc
     4561 cagcgaattt aactatgcaa caccagcatc tgtagatgag tcaactgtcg ttatttcagc
     4621 ttctttagga ggtacgactc cagaaacagt tgaagctaac gcagtagcta aagaaaaagg
     4681 tgctacagtt attagtctaa cacgaagcat tgactcaccg ttaacaaaag atgcagatta
     4741 tgtaatttat catcgatttg cagagaacta tgctgctaaa ttagaaaaaa caggctactg
     4801 tttagaattg gcagtagaac ttttacatca agtagaaggt tatgaccatt atgaagcaat
     4861 gcaagaagga tttgctacta tctatgatct tgcccaaaaa agtgcagaat cagctcgtaa
     4921 agatgcgaag aaatttgctg aacgctttaa agatgaaaaa gttatttatg taatgagtag
     4981 tggtgctaca catgaagtag catactcaac atcaatttgc ttgatgatgg aaatgcaatg
     5041 gattaattct ggtaccttcc attcaggaga atttttccac ggaccattcg aaattgtgga
     5101 taaagacgta ccgtttatat tattaatgaa tgatggaaaa actagaaata tagatgctcg
     5161 agcattaact ttccttgaac gctttgatac gaaaacagaa gttgtagatg ccttggatta
     5221 tggcttgtct tcacatattg ctaaagaagt cttagactac tttaacccat tcgtgattac
     5281 tgcagtattt agagtttatg cagaagaatt gtcagaagca agagaacatc cattaactaa
     5341 gagacgttat atgtggaaac taacttatta aaaaatagat caaactaaat aaggtggctt
     5401 gagaaattaa gccaccttat tttcttcaat gtgattttat acatatggta aaataactag
     5461 tacatattgt gaaagcaggt ggcaagatgc agcgattaaa tgactatctt tatcaactaa
     5521 cattgctttt gaaatctacg ccttcagata attattcagc tcgcagtatt tcgcaagctt
     5581 tacagttgaa tagaagtaca atcagtagct acttaaatga aggtgttcgt gaagggcttt
     5641 tcataaaagt aaaaagttac ccggttttat ttttacatag aacagctctt gaagaactac
     5701 atatcacact gaataactcc gaaatagaat caattgaaag cttgttaaca atttctcaaa
     5761 aacccgcatt agatcaagta attggctcta aaggaagttt gaaagaagca attgatcaaa
     5821 ttaaaacggc agtcttatat cctggaaaag ggttgccttt attattgatc ggcgctagtg
     5881 gatcagggaa aacctttcta gcgaataaaa tatatgaata tgcagtagaa gaaaaggtca
     5941 ttcacacatc ggcacctttc atagattata attgtgcaca atatgtgagc aatccagagt
     6001 tactatcctc tgctttattt ggctacacga aaggagcatt taccggagca agccaggagc
     6061 atactggttt gctagaaaaa gccgatggag gagtattgtt tttagatgaa gtgcatcgct
     6121 tatctggaga agggcaagag aaattattta catttatgga tacgggtgaa ttctctccta
     6181 tgggggataa tagtattcgg aaaaaagccg atgttcgatt agtctttgca acaacagaaa
     6241 atatatatac gactttctta ccaacttttc ttaggagatt gccagttatt gttaatctac
     6301 ctagatttca acaacgtccg tcgtttgaac gtctgtcatt gattgatgaa ttttttgttt
     6361 ctgagagtaa aattttagct aaggaactat ctgtttctga tgcattgatt catttcttaa
     6421 tgaatagtga ttttgaaggt aacgtgggga atattaaaaa tattatcaag tatgcttgcg
     6481 gaaacgcata tatccaccag aagagtcagc aaacaattca agttaaatta ttggatatgc
     6541 ctttagagta cagttataaa ttcaaggagc aactaaataa acccagaaag aaaagagcag
     6601 atcgaatcta ctatccagaa gaaacacgac aagtgcaact agaaagaaac aatcagcttt
     6661 ttgttcaatt ttttgaccaa ttacttactg gattctctga agtgattgaa aataacaagg
     6721 atattaaact atttttagaa gaaatggtta ccaaggtaac acaagtaatg gatacgctta
     6781 tttttcaaga ggattacgaa aaagagcagt ctttatacgc cattctaaac tatcatattc
     6841 gtcaaagtat tgattttatg cgagaaagct acgggtttga acaagatggt aaccgggtag
     6901 tcgctttggc caactatcta tatgtcaagg ataatcaagt gatttttgaa aatagtcttc
     6961 aagaaaagaa aaccaaagat cagcttaacg aattttttgc taatgttctt gaaacccctt
     7021 attggtatgc aaaaaaaatc ttaagccttt tatctgagtg tttagatcaa cctgtgtatg
     7081 aagaggacat tttctttatt acgctatatt tttatagttt gaatgtcacg aatgttaaaa
     7141 caggtattaa gagtattgtg ctagctcatg gttattctac agctagtagt atggcgaatg
     7201 ttgccaaccg tatgctgaat aagaacgttt ttcaagctta tgatatgcct ttagatatta
     7261 cattagagaa aattgaagca aaattgattc aattccttgg agattatcga acagattcag
     7321 gcttgatttt attggtagat atgggttcac taaaccaact aggcggttta ttagtgaatc
     7381 atctaaatgg accactgtta atcattgatc atatcaatac accgttatta ttggaagtcg
     7441 gccaacaagt attaaacaat aaaagtatca ccgaaatagg agcagaaatt gaaacaagtc
     7501 ttactttcaa aaagcagtta ctacttccca agaaaaagaa aaagaaagcc atcattactt
     7561 gttgttatac agggattggt agtgcgaccc agattcagga gattttaacg aaatgcttga
     7621 aagatgcagt tagagaatta gtaattattc cgtatgatta caaaaagctg ttagaaaaca
     7681 agaattttga gactcctttt caattatacg atgtcatcat gattgtaggt acagaagatc
     7741 ccaaaattaa tcagattcca tatattggat tagatcgcct gattaatggg gaagcaattg
     7801 gcgaatttgt tcattacttg gaaaaagagc tgactattga agtgaacgat ttacaaaaag
     7861 aattaatttt caatttttca attagtaaaa ttgtcgaaaa tttaaccatt ttggatccag
     7921 ataaggtgct atcttttgta caaaaagcaa ttaaagaatt atcgaagtta ttaaattttg
     7981 atttttctaa taatcagctg tttttgttgt accttcactg tagttgtatg attgaacgaa
     8041 ttgtacgtaa agaaagtgtg gatgagcaag aggacattga agaatatatg caagcaaatg
     8101 gagaaataat ggataaaatt catagcgctt tttttgagat tgaaagggaa tacacaattg
     8161 aaataccaga tttagagttg cgattattga gcgatattgt tcaaggatag gagtgaattc
     8221 gaatgacaat gggagtaatt tttgatatgg atggggtact catagacagc gagacatttt
     8281 attttgagag aagaatgaac ttctttcaag aactggagat agatccagga tcaaaccaga
     8341 ttgatgatta tgtggggaaa acagaagctg caatatggga aacattagtt cctgaggaca
     8401 aatcattaag agaggaactt tatccgaaat atgtagatta tcgaaaaacg catccaattg
     8461 atttcgctaa agatttacga aaagaagtga aaccgttact tgacttcctc aagaagcggg
     8521 atataccatt ggcactggca tcttcttctc cagttgttga gatagagagt atgctcattc
     8581 aatgtgaact tactgaatat ttttcctttg ttataagtgg tgagtcatta aaacaaagca
     8641 aaccacatcc cgaaatatat ttaaaagcga aacagcaatt agactgtgac ttctttattg
     8701 cagtagagga ttcaacgatt ggtattaaat cagcaaaggc agcaaatatc tatacggtgg
     8761 cactaaaaca accaatgcaa atcgatcaga gtgaagcaga catacaaatt gaaagtctac
     8821 aagaactgga acaaattgtt ttagaaaaag gttagtgaat taatggaaat aaggctatat
     8881 atctgtgcta gctattttga ctaaccaaaa aaacttgcta taattcaggt gtagtaaaag
     8941 gtgctggtaa caccttttac gaaaatgctt cggcagtgat aatgggattg gctttttaag
     9001 ttttttattt tgctcgcaat ccctaaggtc atgtgaatga tcctagggat tgctagaata
     9061 ttttgcaaca ttctatctat ttcagaacgt aaccttcccc agttcattgg gattcattag
     9121 catacaacca acatttcaaa aagaaattgc actacaccat tattagctat tttgactaac
     9181 cccaaaaaac ttgctataat tcaggtgtag gtaaaaggtg ctggtatcac cttttacgaa
     9241 actgcttcgg cagtggtaat gagtgtggct taaaggacta gtcctttaag ctttttactt
     9301 tgttcgcaat ctctaagatc aagttaatga tcgataagat tgctagaata ttttgcaaca
     9361 ttctatttca gaacgtaacc tttcccattc cactgtgatt cattggcaac cctcctcaca
     9421 accaacgttt caaaagaaat tgtaaagttg ttggggtacc acgaccgaac aatactacac
     9481 ctgaatgata gtaagttgtc tcattaagta tagcatatcc tctttttggg taaatagaaa
     9541 tcggttacat tttttatcca agaccaagtt ttttcatttg gtcttatttg ctatacccaa
     9601 acaattccgc agtatagtcg acggtattga ttttatcagg tgagtaatag aaacttgttt
     9661 tccttcccca aaacattacc tataaaggag aaaaaatggt atcgtaatta tataggtata
     9721 ggatgaaatt tttttctaag aggagggttg ttgatgaaaa atttacagtt aaaatttatt
     9781 gttgatccat cgattgtacg ctggtttcgg attatcaatc aatttgagcg caatcaactt
     9841 gccacaaaag gagaattagc aaagagtaat catgtatctg aacgaaccat tcaaacagat
     9901 gtcaataaaa tgaaggacta cttttctgaa agtgttcaat ttgtttcaac gaaaagtggg
     9961 tattcttttc agaaggaaag accggttctt tttctcaagc agaaggagca actactggag
    10021 aacgaaattt tatttgagtt gttagggaat gtcttttatg gcgaattgga agatatgacg
    10081 gagctaattg atcgttttca tttatctgaa gcgacttttc gtcggtattt gaagcagatt
    10141 caaccagttt tggaagaata cggcttagaa ttgagtcttt atcctcttga tttaaaagga
    10201 gaagaggcaa atattcgcaa gttttttaaa gacttctatt atgaggggga gatgacgccc
    10261 catactcttg ttccaccaag ggatcttcat gaacttgtgc aagctacatt ctatgataaa
    10321 tttgagtatt tctcgattgg aacagggact tctcctgctg aattttatta tagtttgtat
    10381 attgcgattg agcgtgtgag acaaggaaaa acgatagcca ttcctcaagg tctgattgag
    10441 cgagtaattg ctagtgagaa ttttcgctta atgtattcct tgaaagaagc gattcaaaca
    10501 aattatcaag ttgctttatc agaagaggag ttttgttggt tgtattt
//
LOCUS       DACEPW010000087        10496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEPW010000087 DACEPW010000000
VERSION     DACEPW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.088"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1122)
                     /locus_tag="IW819_001723"
     CDS             complement(<1..1122)
                     /locus_tag="IW819_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1244826.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKF"
     gene            1374..2897
                     /locus_tag="IW819_001724"
     CDS             1374..2897
                     /locus_tag="IW819_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1244827.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVASI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFITQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYIEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(2966..3694)
                     /locus_tag="IW819_001725"
     CDS             complement(2966..3694)
                     /locus_tag="IW819_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1244828.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(3707..4987)
                     /locus_tag="IW819_001726"
     CDS             complement(3707..4987)
                     /locus_tag="IW819_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1244829.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKTLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLVVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(5128..5730)
                     /locus_tag="IW819_001727"
     CDS             complement(5128..5730)
                     /locus_tag="IW819_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1244830.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5975..6181
                     /locus_tag="IW819_001728"
     CDS             5975..6181
                     /locus_tag="IW819_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244831.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6493..7212
                     /locus_tag="IW819_001729"
     CDS             6493..7212
                     /locus_tag="IW819_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1244832.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(7293..7976)
                     /locus_tag="IW819_001730"
     CDS             complement(7293..7976)
                     /locus_tag="IW819_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1244833.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            8222..10408
                     /locus_tag="IW819_001731"
     CDS             8222..10408
                     /locus_tag="IW819_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1244834.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         3822 a         1792 c         2007 g         2875 t
ORIGIN      
        1 aaatttattg tagcgttgtt tgaatttacg gaaaataaaa gtaacgagta atttgactaa
       61 cgtatagata ggaataccaa aaataacgcc cacaattccc attagatcac ccatgacaag
      121 taaaacgatt aagatcgtaa tcggatggat ctgtaaacga tcacccatta ctcgaggaat
      181 caccaagtca ccgtggagaa gttgtacggc aaaccagaca atgacaaatt tgacagccat
      241 aaaagtagag tcttggaatg caatgatcaa acctggaaag aaggcgataa acggcccaat
      301 atatggaatg atacataaca acccagcaga aatagctaaa acactagcaa attctaatcc
      361 gatcagtaag aaacaaaccc agtagactgc tccaaggata aatgaagcaa tgatttgccc
      421 ctttagaaat gaacccaatt gttgattagc aatctttgtc aaatttttaa aatctgtacg
      481 aaatgctggt gggacaatcg ctaaaactga ctgataaaat ttttgaggat ctctcaaaag
      541 gaaaaaagcg acaattggac cagtaaataa agtaataaaa atagctgtaa cagttgaaaa
      601 aatgttgctg atccgttgtg cgccactttc ccaataattt ccaataaatc cagctatatc
      661 agacgtcacg tcgtcaatag aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt
      721 ctccaaaaat tgtcggagac tctcttgaaa atcttgaaac aaatctggca aactactgat
      781 caatgtcttt gcctgttctt caagaaaagg aacaagccat acgcttccaa gccccaataa
      841 aaataaaata atcacataaa cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa
      901 acgtttgatg actggttcca aaagataata caagatcagt gcaaacaata atgggggcaa
      961 gatagtagcg ataatgattc cgacaggctt gaatataaac gagatctgat ggaaaaaata
     1021 aatgatcagc gcaagtaaga taactattcc taaaacatag taagaggttt ttcctcctaa
     1081 aaagcggatc aaacgattcg ttgtatctgt ttcttttttc actctttttc ctcctttatc
     1141 tataacttca ttatagacaa aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa
     1201 aatatcgtca atcattcaca taaactttct catcaattaa aataagaaaa cgcttgacag
     1261 ttattttaac catatgataa attaaatcct gttgctaaaa cgtaactatt acggtttaca
     1321 aaaggcgaat cggaacattc aaatgaaaga agatgagaag aggagtgtca taaatgaaac
     1381 gattgatagg tatattggcc atgctggttt tagcaggtat gttaactgct tgcggggcaa
     1441 gtgggaaagc cgaggacagt aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt
     1501 atgattttac aaaagcaatc gtcggggatg aaggagaagt agagttattg attccagcag
     1561 ggactgattc ccacgattat gaaccttctg ctaaagacat ggcaaagatc caagatacag
     1621 atatctttgt ttataatgat gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat
     1681 tacaagaagg caatgtccat acgataaaag cgacagaagg tatgctttta cttccgggaa
     1741 gtgaagaggg acatgatcat gaccatgaac acggggaaga aggccataca cacgagctag
     1801 acccacatgt atggctagca ccaagtttag caatcaagca ggtagcaagc atacgtgatc
     1861 agctgattga agcttaccca gaaaaacagg aagtatggac aaaaaatgca gcagcctata
     1921 ccgagaaact tcaagcactc catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa
     1981 gatcttttat cacgcaacat gctgcattca actatctggc attagaatat ggattgaatc
     2041 aagtatcgat tgctggtctt tcttctagtg aagaaccgtc tgcggcgaga atcgctgaac
     2101 tgaaacactt tgttaaagaa catggaatca actatattta ttttgaagaa aacgccaaag
     2161 atagtattgc cagaacgtta gcaaatgaag caggtgtatc tttagaagta ttgaatcctt
     2221 tggaaggatt gacgaatgaa caaatagaaa atggagaaaa ctatctttct atcatggaag
     2281 caaacctcga agcgttgaaa aaaacgacag agacagaaaa cccgttagaa gatactttga
     2341 cccccaaaaa agaaaagagc gtctataacg gttatttcga agacgaggat gtacaagatc
     2401 gctctctttc agattgggca ggaacttggc aaacagtaga tacgtatata gaagatggga
     2461 catttgatcc tgtttttgaa tataaagaaa aattgaatca ggataagaca gcacaagaat
     2521 ataaagaata ttatacgaag ggctatcaaa cagatattgc ttcaatcaag atcagtgaga
     2581 aagagatggc atttacgttt aaggatggaa caacgaaacg ttcaaattat cgttatgtag
     2641 ggaaagaaat cttgacctac gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag
     2701 aagataagga aagtggcgct tttagatata ttcaattcag tgatcacacg attgcaccgg
     2761 ctgactccga acattttcat ttatatgcag gaaatgaaag tcaagaagca ttgctgaaag
     2821 aaatggagaa ttggccaaca ttttatccag atacaatgga tggacgtgaa attgcacagg
     2881 aaatgctttt gcactgatag gcaaaaaaga accgaagggt ttggatcggc tggtcattta
     2941 gtcggtccaa actattcggt tctttttatg aaatcaattt ctttaatcca cggatagagg
     3001 ctgggatggt ctcgccgttt gccaacgtga cttctctggt ttttttatta atcgaccgca
     3061 cattttgtgt attgataaca taggatttat gacaacggat aaaattccga tgcattttct
     3121 cgatttcttt gatcttctca tagaattgta attgggcatg agtcaaatgg acttccagtt
     3181 tatgtggtgc tcctagcgaa gtagaaaaat attggatgtc tttgatcggt aaaaatttga
     3241 tctcatcatt aaactctatt ttgaagatat ctttttggat ctgggacatt tgaaaatctt
     3301 cgaaaatttt gctcaggata tgctcgacat tttcttttaa atcataaaga tcctctttga
     3361 cgatataatc agtcggttca atgttgcttt ttaagatttt catcgacatt tccgtatggg
     3421 aagtcacata gattattttt cctaaaggat ctaattcacg gatcaattta ccgatcgcta
     3481 caccatccat ttcactgtct tctagttcga tatctaaaaa gtaaatacca aaagaagaag
     3541 ttctgatctt ggctgcattg agaagttctt ccggatcaga ggtagaaata tggacataac
     3601 tgtctaagtt ttcaatcatg attctatttt tgatcaccgt tgataaaact tctaattgtt
     3661 ttggatcgtc ttcacaaata taaatgggaa tcatatcatt actcctttat tttatttgta
     3721 tataaatcgt ttgagaaaaa cgctggtttt caattgatgt ttcaaggaaa agataaggat
     3781 tggtctttgt caattcatcc aagataaaca agccaaggcc ttcgtttttt ggatttgttt
     3841 ttgttgagaa ccctttttgt ttcaactggt ttaacggcaa tgtatttgtt gttgtattga
     3901 tcacacgaat catcacggaa tgattcttct tgagtatggt caattcgatt tttttcttct
     3961 ttgcagcact agctccttca atcgcattgt ccaagataat accaagcata cgtacaaggt
     4021 ttaccgtatg tttcgtatcc agttcgattg gctcaggaaa atgaagctgg acgaccaacc
     4081 ctttttcctg tgccatcatg agttttaatg acagtaaact acggatctca ggaactttca
     4141 tattatccaa catacttaga ttggccggaa aaaggttcat catctggcgt gtaggtaata
     4201 tcgtttgatc ataataagac cgtaatcctt ctaagtcgcc ttctgaaata tagctgtgaa
     4261 gagaaaaaag cagattttgg taatcatgtt tgaattctct caagtcttga tagttttttt
     4321 ctagatcttt gatataatcc tgttgcatcc tgacttgttg ctctaataac tgatcagcta
     4381 atttcttatt agagattttc caataataaa ccaagatgat caggccaaca cacagatata
     4441 gaatgaaaaa actcgtattc aaagctaaga catcaggtgt ttcaccagaa aaacgggtat
     4501 aaaaaataat gatatagtat gtaaataata tcaatgtccc gatcgtaccg atcatacggc
     4561 ggtccacagt gtgaaaccaa gtctctaacg tttttttaaa acaaaaagct aataaagcag
     4621 ataaaaaagc ggataaggca atgtgaaaat actgcaatga atctcctggt tcaataaccg
     4681 aagatttcaa aacaaaaaaa tcgccgatgc ttgttaagtg atctcctaaa agcgacgtca
     4741 ataacccaaa aggaagggcg taaaccaatt gttgacgcga agtcgaaaag aagaaaaaag
     4801 taagcatcag cattcctata gcaattagag cagtaaaaat ccctaaaatc gaatagaccc
     4861 atccaatcaa aatacaagaa atcagaatga ctcccgcaaa cagatatttg tatcggatgt
     4921 gatggtcaat caataagata caaattgtgt aaatcaaata ttgcaacgct atggaccata
     4981 aaaacataaa gtacagctcc cattgatttt taccaaatta tagcacaatt agcgtattat
     5041 ttttttaatt tttcagggat tttgaattca gaactcatac caaagcagca gccagataca
     5101 aaaaagtcag ataagttagt taattgttta atgattgttt ttttcatgaa agattcctcc
     5161 aaaattcatc aagtagtttg taataggaag caacgtagct atttctaaaa caatcccaag
     5221 tagcaaatag gtagaaaagt aagaagctga gaaaaataaa gtaatgagag aaaaaattag
     5281 aaacatgctg atcgccttct ttaataatgg gggacgtttc tcgggttcaa tgggattgat
     5341 tgctgtacct cttgatccat agcgaagcag taacaaaaag cagctgatta acaaaaagac
     5401 catcattggg aaggagatag tcaattgcag atgtttcatc accaagggaa cgccccataa
     5461 ataaagtagt gtccacagca gacaaacgaa ttcagagtcg gaatgcaatc cataagcata
     5521 tctgcggatg cagatataag aaagaaaggt gattcctgtt tccgcccatg agcctgtcac
     5581 cagagaaaag actagaacga ccgctagttt tccaagatta ttcaataaaa cttctaaggc
     5641 aaattttact tggacataca ccatatcttc ttccgatttg tctccgctga ataaggcttc
     5701 taataatttg ttcacaactt tatctttcat ctcatacgta cctccaccac aaattttatg
     5761 ctaaaaaaaa taaaaaacgt cttttgtttc ataaaaagtg gttttgacat caaaaccgtc
     5821 atgatttcct aaaaaaatct tcgttttcca agaaccttac caaaaaaagt gacagttttg
     5881 atacgaaaag tactttttat gacaagtttg tgctttttcg agtgatttat gttaaaaaat
     5941 tactgacctt ataaaaggtg atggagggat tcttatggaa aatgaacaat tgtttgaagt
     6001 cggcgaaaca tatgcattta aatctgattt atttgaatca ccaataaaag gaaaaataga
     6061 aaaaatctat gaaaatagtg tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga
     6121 taccgctcat aatctgcact ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata
     6181 aaaacagata aaatgttgag agcataaaga ggttgtgagt ctcccgttta gctctgagtt
     6241 acaaggaata atgttaaaaa acagcttttc atgttttatg aatattgcct tgtgaccgag
     6301 gtgttggcag gagaatactg tttatttgaa aaaagggctg cgacaagact tttgtcacag
     6361 cctttatttt catgaatagg agttactttt ggctactttt agagaagtag catttaagga
     6421 gcagataaag tcaaggcttt tatttctata aatgaagtag taaaatgaag aacagatttt
     6481 attacaggaa ggatgccgat aattgtgatc attgttgcag gaacgatcgg tgcagggaaa
     6541 agcacactga ctgaaatgct tgcccaagat ttagaaacaa aaccttttta tgagaacgtg
     6601 gaagataatg aagtattgcc gctattttat tctaatccag aaaagtatac ttttttatta
     6661 cagattttct ttttgaacaa acgctttttg gcaatcaaag atgctttcag ccatgatgac
     6721 aacgtactag accgctccat ctacgaagat agtatgctgt ttcatttgaa tgcagattta
     6781 gggcgtgttt ctgaagtaga agtaaagcag tatgaaggtc ttttagaaac gatgctgaag
     6841 gagttagaag aaatttcgcc acaaaagaaa ccagatctac tggtttatat tcgtgtatcc
     6901 tttgaaacta tgttagcgag aatcaaaaaa agaggccggg aatatgaaca gctagaacaa
     6961 gatcctgaac tctattccta ctataaagaa ctcaatcgac gatacgaaga gtggtatgaa
     7021 cagtttgata tatgtcccaa aatagtgatc gatggggaca aatatgattt cgtagccgat
     7081 ccggcatgtg gagaacaaat cgtacaagaa atcaagatgc gggcaaagaa aatgacagaa
     7141 gaggcagatg cccagtcgat ccgctacact acaaataacc cgaaagcaaa gggaatcttt
     7201 acaactaaat aatataaaaa ctaagcaaat tgctcactcg cagatatttc tctgtgagca
     7261 gcatcttgct tagttttttt gattcattga gatcaaatag tgaaccgatt cgtatgataa
     7321 aattgccata aatgaaatgt ctgagtgagg gccatgatca aaaataaaat ggaaacgatc
     7381 aaaatcgaat gggcgccaaa cagatgatga cagaaaacaa ggacaatagc tcctgcgata
     7441 aaggcaagaa cgacacttag atagtggaaa aatcgatcaa aatctgcttt ttctttagaa
     7501 atacaataat catataagtt cgttgctacc ccttttaaat ttccagttgt aaataaatta
     7561 gtgtaggcac gcccgttgat tttatcaaaa gctacccact ggatcgatgc agtaaaagca
     7621 atcaaaatcg ttactaatgc gggatgctca tggacgggtg tcaaactaac aaagaaaaaa
     7681 atcactgctt cataatatag aacaaacaag cgccagaaat gtttttcttt ttttctaaaa
     7741 taatcaatca gatactttgt tagaagaatc cctaagaaaa agaaaagggt agacaatact
     7801 tttttaccaa cacttagcca ttctccgtca aatgcttgga tgatcgatag aatgatattg
     7861 ccagtttgag cagaggcaaa agcaccatat tggatataag tataactgtc catggctcct
     7921 ccgataaatg tcagcaaaag tcctattgta cgcccctcgt gaaaggaagt ctgcattttt
     7981 ttcatcacct tttatgaata tttttgtctt ccttatctta gcataaacga ttgaaaaaaa
     8041 ggaaaaattt tcagaaaact aaaatcatat cgcttcccat tttcattttt agaaaggtct
     8101 gttagtataa agaaaaaata gacaacaaaa ctttaaataa ccaactttaa ataaccaact
     8161 ttaaataacc aacttgaaat aaacaaattc aaagaagaaa gatgggaagg aaatcaatca
     8221 gttgaatgaa aaagaaattc tccgattaca aaaagaattg aaacgctggg agaacaagta
     8281 taaaaagctt caacaagtct ttcggctaca agaagaaatc attgctacct atgaagcggc
     8341 ttatggcgaa gcacccggac ttattgagga agaagtacaa gtaaaagaaa gtgaaggaaa
     8401 cagctctttc tttaaaatga tggagaaacg ttcctataat cgcttcaatc aagaacaaaa
     8461 agcagctatc acatatgata tgaagaaaca tcttcgaatc atagcaggag ctgggagcgg
     8521 caaaacgcaa acaatctgtg caaaagctgt ttatctgatg acgcaaaaac aagtagacga
     8581 agaacgcata ctaatgatca cttttacaag gaacgctgca aatgaactaa aaaaacgagt
     8641 agataatttc agccagagaa aaaccgctgt ccatatcgga acattccata gtatcttttt
     8701 taggatatac aatgaaattt gtcgaaaatt tcctgaagta gcaatgcaag gaatacaagg
     8761 agacttttca aaggatactg cacaaaaagt gaatgctgtt ttgcaacagc tgatacgtaa
     8821 atataatctt tatatatttg atcaatatgg agaaaaaacg attgcttctc ggttagacta
     8881 ttggcagaat atgaaccttt cagtagaaga aatggttcag ttagttaaag aaaaatatga
     8941 ttccattgat aagaatgcac gacagccgat atcagaacga ctgtatggat tgctcacgga
     9001 actccaagaa caaaaaagaa gccagcaatt gctagagttc aatgatgtac tccaaaatct
     9061 gaaaagcgct ttggagaatg aggagatcca gcattatatc ggccaaaaat atgattatct
     9121 attcattgat gaatttcaag atactaatcc acttcaatgg gaaatcgtga aacggatgac
     9181 aaaggaaaac aggatcaagt tgattgttgt tggagatgat gatcagagta tctatggatt
     9241 cagaggatca gagccagcat acatcaagaa ctttgaaaaa gaatatccta ccaaaactct
     9301 tttcttatta actaattatc gttctcgggc agctattgtc caaggagcaa atagattgat
     9361 cagctataat aaaaatgatc ggataccaaa ggcgatgatt ccagcacaaa aagaagcagg
     9421 tgttatggaa gcctatttgt tttctgatac taaagcagaa agtcagtggc taatcaaaca
     9481 aattcagaca tttattcaaa aaaatgggaa atataaggaa tcaatcatat tataccgatc
     9541 agcttcacaa acgcagcagc ttgtccaatc tctgctgaaa acgaatattc cttttgtact
     9601 ggaagcagat agtccatatg aaggtatctt tggtatcaag tctttccaac atttctatca
     9661 aaaaattgtt cattggcaaa cagcactgaa tctgcaaaag aaaaatcagg cctaccagca
     9721 attgctccgg cagctgatgg cagactgcta cctgaaaaaa agtgaatgcg atcaatactt
     9781 ttctcctaaa tatcaaaata tagccgttgc agattatatt ttgtcgataa gccccaattt
     9841 aaaatcgaaa tctgcagaaa tccgagagtt tgaggaagcg ttgcgtaaaa cagaccagca
     9901 tcaagaaatc tattcgcttg ttcaaagcta tcttcatctg ccacgtatag ctaaggaaat
     9961 cgaccagagt gatcaagaat ggatacgaga agaagtaaag cagcatgcta ctttcagaag
    10021 actccaagcc ttatcgcaag aaacccaagc gacagctaaa gaattaaaaa cccgcttgat
    10081 tgcttacaga caaggaaaac ttgatgcact atgtatccag agcattcaca aaagtaaagg
    10141 tcttagttac cgaaatgtct ttttgattgg ttgtaacgaa ggaagtttgc cttataacgg
    10201 agcgacagaa aagaaagtaa tcgactttaa tgaaataaaa gcagaaccag tgacgacgat
    10261 agaagaagaa cgaagactat tttatgttgc gatgactaga gcaagaaatc gtctgtatct
    10321 atgtgtacct caaatgaaaa attcaagaaa attgaaagta tcgagattta tcaaagaaac
    10381 cgggagtcgt gtgaaaaaag gaaagtgata gaagagaggg tgtgacagaa gtggtcagtt
    10441 tcaagaaaga agaaggaatg tacgaaaatt gcttctcaaa tttttgtgaa acttca
//
LOCUS       DACEPW010000088        10316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEPW010000088 DACEPW010000000
VERSION     DACEPW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.089"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..816
                     /locus_tag="IW819_001732"
     CDS             4..816
                     /locus_tag="IW819_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1244835.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IW819_001733"
     CDS             813..1613
                     /locus_tag="IW819_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1244836.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IW819_001734"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IW819_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1244837.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IW819_001735"
     CDS             3110..4819
                     /locus_tag="IW819_001735"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1244838.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="IW819_001736"
     CDS             5031..9389
                     /locus_tag="IW819_001736"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1244839.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="IW819_001737"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="IW819_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1244840.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..>10316
                     /locus_tag="IW819_001738"
     CDS             10030..>10316
                     /locus_tag="IW819_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1244841.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRF"
BASE COUNT         3407 a         1773 c         2311 g         2825 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttga
//
LOCUS       DACEPW010000089        10289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEPW010000089 DACEPW010000000
VERSION     DACEPW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.090"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..769)
                     /gene="xth"
                     /locus_tag="IW819_001739"
     CDS             complement(17..769)
                     /gene="xth"
                     /locus_tag="IW819_001739"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1244842.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            882..1802
                     /locus_tag="IW819_001740"
     CDS             882..1802
                     /locus_tag="IW819_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1244843.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            1875..2810
                     /gene="murB"
                     /locus_tag="IW819_001741"
     CDS             1875..2810
                     /gene="murB"
                     /locus_tag="IW819_001741"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1244844.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3108..4586
                     /locus_tag="IW819_001742"
     CDS             3108..4586
                     /locus_tag="IW819_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1244845.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4863..6923
                     /locus_tag="IW819_001743"
     CDS             4863..6923
                     /locus_tag="IW819_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244846.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7006..7575
                     /locus_tag="IW819_001744"
     CDS             7006..7575
                     /locus_tag="IW819_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1244847.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7750..8556)
                     /locus_tag="IW819_001745"
     CDS             complement(7750..8556)
                     /locus_tag="IW819_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244848.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8694..10208
                     /locus_tag="IW819_001746"
     CDS             8694..10208
                     /locus_tag="IW819_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1244849.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3650 a         1714 c         2032 g         2893 t
ORIGIN      
        1 ctttttcaca acctctttat ttcaaatcta attctactgg acaatgatca ctgcccataa
       61 tgtccgtatg gatctttgca tcagctagtc ctggctctaa atctttgctc gtcaaaaagt
      121 aatcgatccg ccaaccagca ttgtttttcc ttgaattgaa tcggtagttc caccaagagt
      181 agacaccttc ctgggttgga taaaaataac ggaatgtatc gataaacccg ttatccaaca
      241 gtcgagaaag agccgctctt tcttctggag tgaatccagc atttttttga ttcgttttcc
      301 agtttttcaa atcaatcttt tgatgagcga catttagatc cccacacacg atgactggtt
      361 tttgtttttt tagattttcc aaataattat aaaaggcttc ttcccattct aatcggtaat
      421 ctaaacgttt taattcgctt tgagaatttg gcgtgtaaca ggttacaaga aagaaatcgg
      481 aatattccaa agtaatcaga cggccttctg tatcatggat atcgataccc attccgtaag
      541 tggcattcaa tgctggtttt ttagcaaaaa tcgctgtgcc ggaatagcct tttcgttccg
      601 cgtaattcca atattgataa taacctggaa gatctaaatc aatttgtcct gcttggagtt
      661 tcgtttcctg cagacagaaa aaatcggcgt ctaactcatg aaaaacttct aaaaaatttt
      721 tatttacgat ggcacgaagc ccgtttacgt tccaagagat aaatttcaat aaaagcacac
      781 tccttcgaat taatttcctt acctttgcga tactattgta cctcatttta gtaaaaaatg
      841 gtgtaagatg aaagtaacta aatgatccag gaggaaagta gatgaagatt ggtgtaatcg
      901 gtgtagggaa tatcgcagaa aaagcatatt taccaactta tgcgaaaaaa caaggaacac
      961 ttgattttta ttttgcaaca agaaataaag aaacaaaaga aagaattcaa aaagagtacg
     1021 ggttccaaca tctatatgaa acgatcgatg agcttttaga agaaaaaatc gaagcatgta
     1081 tgatccacgc agcaacaaaa gtacattatg aattagccaa aaaatgtcta gaaaatggta
     1141 tccatgttta tattgacaaa ccactgagtg tccatctaaa tgaagttcgt gaattgcaag
     1201 aattagctga taagaaccaa ataattttga tgatcggttt caaccggcgc tttgctccta
     1261 tggtagaaga attgaaacgt atcccagata agcggttgat ccacctgcaa aaaaatcgaa
     1321 tagcggcaaa agaaacgaca gagttcgtcc tttatgattt gtttttacat cttattgata
     1381 cagctgtgta tttattagat gaacccattt tgagaacaaa ataccagata agagaaacaa
     1441 aggaattttt ggaatatgcc attttacaat tagaaacaca aaatcaaacg gctattttaa
     1501 cgatggattt agcaagtggc gctaatatag aaaaatatca agtaacaagt aaaaatggta
     1561 cttatgaagt aaacgatctg acccagcttg tcattcaaga tgctgatgga aaaaaagtga
     1621 aagaatttgg cgactggaca aacacgctag tcaaacgcgg gtttgagcca atggtagagg
     1681 ccttcttttc tcagatccgt ttaggtaaat cggaaacaga attgcttaaa caaaaagaag
     1741 ttgtaagaag tcatgaatta tgtgaggaag tgcttaaaga tcactaccgt catcaacttt
     1801 gaacaagact ggcaagattg tattaagcgt gatagaataa tgaggacgtt tgagaaaaga
     1861 aaggaaaatc ctcagtgaat aaagaagaaa tagttgaaag cttagccacg attactatat
     1921 tgtttgatga accattgatg aactatacgt ttacgaaaac aggtggacca gctgatgtcc
     1981 ttgcattccc gaaaaaacaa gaagaagtaa agcaaatcat cgattattgc cgaatccatg
     2041 atataccttg gatggtatta ggaaatgcaa gcaatctgat cgttcaagat ggcgggattc
     2101 gcggtgtagt catcatgctg actgaaatga aacagattca tgtcaaagga acgatggtca
     2161 ttgcggaagc aggtgcctca ctgattgata cgacttatgc agctttggca gaatcattga
     2221 ctggatttga atttgcttgc ggcatcccgg gaagtgtcgg tggagcggtt tatatgaatg
     2281 caggtgcgta tggcggagag atcaaagatg tttttgctga agtggatctt ttgcttgaag
     2341 atggaacatt gaaaactttg acaaaagacg aaatggtatt ttcttatcgt cacagtaaag
     2401 tccaagaatt acgtgctatc gtattggaag ctcgattcag tttgcaaaca ggggattatg
     2461 aagcaatcaa agcaagaatg gatgagttga ccgaattgcg tcaatcaaaa cagccattag
     2521 aatacccttc atgcggaagt gtctttaagc gcccagtagg acactataca ggtcagctga
     2581 tccaacaagc aggattgcaa ggcctgaaat ggggcggtgc acaagtctct gaaaaacatg
     2641 ctggattcat cgtcaatatc gatcatgcga cagcgacaga ttatattgaa ttgattgcgc
     2701 acatccaaga agtaatcaaa gaaaaatttg atgtgtcttt agaaacagaa gtgcggatca
     2761 ttggagaaaa acaagcaata tcagaaacaa gcagaagttt agccaattaa taagtaatgg
     2821 aacttaaaat aagtaaaaga gcaggacggt ggccgtcctg ctcttttttg tggttgttta
     2881 cgcaaatggc gcaaatcttg tacattttaa cacaataaaa caaaaaatat agaatatttc
     2941 ctcataaatt tttccatttt ttgcaatatt ctcaaaaaaa agataaaaaa tgagaataac
     3001 tgatgaaaaa acaagcataa tccatcataa aatcagtata ggtgaaaaga tgaactttca
     3061 tctgcatatc aagtaattgg tatgaggcat tttttgaggt gtaggatatg aagttggatt
     3121 accttataga agaggaagat agagaaaaac tgctgctagc tagatattta gaatttgaga
     3181 aaaagaatta tgtagtgctg cctaagttat atgaaacatt aggtttgtct aaatttaaaa
     3241 caagaaatta tatcatagaa ttaaataaag aatttactga attcaaaaca aagccagaga
     3301 tccaaatttc agaagaagca gagatccatg tcgaacgtct gaatttgacg gatatcaaaa
     3361 atttaagaag aatctacttt caacgatcaa aaattgctcg actgttaacg gagatcgtgg
     3421 attcaaaagt aacgatcgaa aaatttgcag agaatcattt tttaagcata tcaagagcct
     3481 atgtaaaaag aagagaatta gtctcttttc ttgaacagca tcaaataaag ttaaagaaaa
     3541 acatattagt tggggaagaa ataaacatac ggaacgtatt atttggggta tatttcgggg
     3601 tctttaatgg tttttctatg ccatttagag aaagcgtcat gaatgaagca aagagtatcg
     3661 tgcaatattt tgaatatctg ttcaatttaa aactgtctgc aacaaaacga atcaaattca
     3721 atttgctaac cactattagt ttgttgaggg cctatgggaa tcaaccaatc ggaaaacgtt
     3781 tcttttcaga tgaatacttt aagagctcag aaggaatgaa gatcatcaag gatttatcac
     3841 atatttcttt tcttcatatc cctaaagagc agatcattga tgagatttct tatatcatgt
     3901 tgtttttgat cacggaagaa gaatttgaat tagatggcgc atttggactt gcggaagctc
     3961 attttcatga tatccacatg attagtcaaa aaatgtcagt ggaattgatt gatcaattac
     4021 cattttcgat tgaaatctct gaagaagaaa gactatatat acgaaaagaa cttcgtatgg
     4081 gtatgatgca tgtcaatcga aaaaattgga tattcgactt tgagatgact agctttacgt
     4141 ctaaaaagca gctacagttt ttcttcgaga cgtatccaat attcagtaca gcaatccaaa
     4201 aggtcataca agagaataaa cgctatttct tgggaaaaga tgaacgtttc atgattcgct
     4261 tattttatga ttatctcttt ttattggtcg gtatttgtcc ggtatcatat tttgaagcac
     4321 cgattcatgt ctgtattgat ttttcgcaag gatctgctta taccgattac attatttctc
     4381 aagtgaaagg atttaaaaat tacaatattg tgattgagag tcgaatcact tctcgtacga
     4441 atatttattt atctgattgt ttacttgaaa aatttaataa agaacagata atttggaaaa
     4501 atccaccgac tccagatgac tgggaatatt ttggaaatac catcatcaga gaaaaaaata
     4561 gacatttaac tgaaaaaatc agctagtatt tatggatggc agctttgtct cacatattta
     4621 ttagctgatt tatgtttttt tcaaagaaaa ggtaactaga tataactaga tataactaga
     4681 tatattactt aacaaaatta aaaagtacat aagttattat tccgattttc ttgaaacaat
     4741 aaaaaatcaa aaaaaatact ctcctaatag tccatcttct tttgatttta tttatataat
     4801 gaaatcatct tatgaaaatt actacttcat gtttagacaa gagtagggag gactaagaat
     4861 aaatgtgttc aaaaagacgt tcaaaacaac taaataaaca cgcttgtcgt ttagataagg
     4921 aacggtcgtg ttctgaaaaa cactacgtta agtttatgat gcgcacgatg ctgatcagtt
     4981 tatctgtagg tgtctatatg catgtacccg ttcaggcaac gacacaaacc atcaaggtcg
     5041 tacgtacctc tgtttctgag accactgatt cccctatcac tattactcat actttcactg
     5101 atgcaaatgg aaaacagcta cttcctgcat tgacttccta taataaagtc agttatcctc
     5161 caacgataaa ccactactgg ttaattccta acaaaacatt atggacatcg gaaaccggaa
     5221 gcagtgtgcc gctttctatc ttttccgata tgactgatcc tacaaaaggt gatcagttaa
     5281 aacagatcac tgatattttg aatcatgctt tgacagatat aaaggacggt ggaagcgcac
     5341 aagtttctta tgtatatgaa aaagattatt cacagtttga aagaaagaaa atcgatgttc
     5401 tggcagggac aaaagtttcg atatctgatc tggtagaaag gctgaagaac gcagatggaa
     5461 cacaaggaga tatgtccaaa gtaatttcaa acaatgggaa tatcgtggat acgactaata
     5521 caggaacatc tactatttcc ttgtcctatt ttgatccaat tgctttaaaa acaatgactg
     5581 catctgctgt gatccatgtt cgtgctgatc aaacagctat caaaggaaaa gatcttcact
     5641 taagaacagg agagagttac gcccctaaag atcttgttga atatgtaaca aaaagcgatg
     5701 gaacttcagg aaataaagaa gaaatcaaaa tgaacgggac agtagatact acaactccgg
     5761 gagaatatcc cgttgtatta acttatatag actcgaaaac atcaaatgtg gcagaaacaa
     5821 cagctgtagt ttatgtagaa gcatctgctg cttcatcgac tgtcatcact cattttatag
     5881 atagtcaaac aaataaagaa cttcaagcag aaacacatat gagtagtgta aatcgtattc
     5941 ctgatttgcg cctacctgct gaattttctg atcgagtgcc aaatatagat ttatcgactt
     6001 atgaacaaaa tgggaaagtc acttctattt ctgattggat gcaagcaaac ggattagaca
     6061 gaaaaagtca ctggtcggag atccttgaag tacttcactc agaactgatg aataatagga
     6121 agcaatcagg aggggaaatt acctttactt atgtttatat accagacaat cgttcattag
     6181 aaggtcatga tcttattgtt catccaaacg aaaaaataaa caaagaagag ttgatccaat
     6241 cagcaacaaa ttccttaggt aaacccgtac cgttaaagga tgtatcggtt acaatcaatg
     6301 aagtaccatt ggaggatggt tatttgacca aacaaacagg tgattttacg attatgtata
     6361 gttatcatga cccgtattca ctccaagaac tccatgctca gtcactatta catgttttac
     6421 cattaaacaa acagatagat caaaaagaac aaaaaagaca agaagaaagc ttgtcagaaa
     6481 aaaaacaaca aatggctcat gcagtttctc caacagggaa agtaaaacaa acaagaagcc
     6541 tagagttagc aagaatatct agaaaaatct cagaatctaa taaaacgata aggcaagaag
     6601 ctcatttggc cccacaagtt tctggattgg aaaatgaaat tttagagcct aaaataaaac
     6661 aacaatcaga aaaagaattt gttgctaaac agtcggaaga tcagaaggaa tcagatgacc
     6721 agaaggaaac gaagaaaaaa cgcttgtcaa ctattgattc caccgatgag catctgcagc
     6781 cgcaagtacc acttcctagt ccgggaacac ccgcacctgg tggaggtatt ccttcttctg
     6841 ctttaggaga tttcatgcga ggtatggcag gaacatgggt atacgctaat cgaaatgaat
     6901 ttgacgcaga tgactggttc tgatttatat ctctatctgt tatagtcaaa taagtagctg
     6961 agtcatatgt tatttggcat aatggagacg aggagggata gaaaaatgtt agataaaacg
     7021 ttaccttacg cagaaatctg gatgacaaga ccacttcaac aaaatgttcc aatcacgcct
     7081 ttagcggaag gttttgaatt tcgattttat caaaaagaag atgaatctgc ttgggcggag
     7141 attgagactt ccgttgggga atttcaaacg acagatgaag cacttgagta ttttcaacaa
     7201 aactttgctc cttatcctga agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa
     7261 ggagaaaaag tagcaacctg taccgcttgg cgaaaaaaat caagagagca agtgtatccg
     7321 gtttttcatt ggttagcagt gaaaccagat taccaaggaa aagggctcgc aaaagcattg
     7381 actgccagag tcttgcaaga atttcctacc ctccatacgg acggaccaat ctatcttcac
     7441 acacagacat ggagccatca ggcgattgct ctttataaaa aaatgggttt tacttttatt
     7501 gctgaaaatc tagacggcac acctaatcca gattatgaaa aagttatgca ggttttaagt
     7561 aaattagata aataacgaaa tggtgaaata agtcctcagt attgggataa aaaacgaaat
     7621 agtcattcct catatcttac ttttttgagt taatactatc ggataactaa tggcatgcca
     7681 tacattcaaa aaagaagggg cagtgacaaa agtattgtca ctgccccttc tcttgttttt
     7741 tagcatgttt tatcgtttga tttttttagg aataaaattc ttgatgaacg taaatttttg
     7801 gtaatcgttt cgactataga ggtaaccaaa tacactgaaa acaactgctc caataaaatt
     7861 aacaaataaa tccttcatag tatccatgat tcccacgtct aagtaaccat tttggatgat
     7921 tgttttatct gttttaccat ctgcattttg actttcaatg attgtccgag tgatatctgg
     7981 aacatgatag acagtgtttc cgtctttact taactccaca ctattgattt gctggatgat
     8041 cgtatctttt tgcatatcga attgcagtaa tttatctgct gaaaattcca caaactccca
     8101 cataacacca actgtcatcg aaaaacagaa ggaaacaact gctagtaata aaggagtcaa
     8161 gttcatggtt tttgtatttt tattgagcaa ataaacaagg gaaaagccga cacccccagc
     8221 taaaaatccg ctgaatgcat gtagaaaagt atcccacatt gaaaaatagc cataaaaatc
     8281 acttaactct cctaaaactg ttgaactaaa aataaaagat aacaaaatga tttctagcat
     8341 atttggaatc tgtatcttta atatcttttc cagaaaataa gggatttcaa ataaaattaa
     8401 tgtcagtaat aaaagcattg tatttcccca gtgttggttc cagacctgat ccactagcat
     8461 aataacgacg atgatacgta ataaaatata gattccgttt gtgattctac ctgcagtttt
     8521 tgtactgttc gtaaaaaagg ttttcaaatg attcataaaa taactccttc atgtatatcc
     8581 ttaattatac taagaagcgt gtgtttacca aagctatatg acttaaagaa aaacagagtt
     8641 gatattgtga aaaaaatcac ttaatatagt ataaggagaa aagggggata caaatgaaaa
     8701 aattagttgc tggtttatta acagccgttg cacttcttgc tatgagcgca tgcggcgcag
     8761 atcagaaaaa tacagcgcag acaagttcta gtacgaagga atctgccgaa gtatcatcag
     8821 gcgcttctga acaagtatat acagatccaa aagaaatgaa agaatcttat gatgtcgtga
     8881 ttattggagc tggtggtgca gggatggcag cagctatcga agcaaaagat gctgggttga
     8941 atccagtaat tttggaaaag atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg
     9001 gtatgaatgc ttcagaaaca aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg
     9061 ccttctatga agaaacgcta aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat
     9121 actttgtcga tcactctgct gatgcggtcg attggctaga tcaaaacggt atcaaactgg
     9181 acaacttaac gatcactgga gggatgagtg aaaagaggac acatcgtccg tctgatggct
     9241 cggctattgg tggatactta gtggaggggt tattgaataa cgtccatgag cgggaaatcc
     9301 ctatttttgt caacgcagat gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag
     9361 tgactgtaca tgttcaagga gaagaaccaa aagaggtcac cggaaaagca atcgtagtta
     9421 ccacaggcgg ttttggtgcc agcaaagaat ttattgaaaa atatcgtccc gatttagctg
     9481 attatattac gacaaatcaa gaaggatcaa ctggagatgg tatcaccatg attgaaaaat
     9541 taggtggaca aaccgttgat atggataaaa tacaaattca tccaactgtg cagcaggatg
     9601 agggggtctt gattggtgag gttgttcgcg gagaaggagc aatcttggtc gatcaagagg
     9661 ggaaaagatt tgtcaatgaa atggatacaa gggataaagt atctgcagca attacagcat
     9721 tgcctgaaaa atcagcttat ttgatttttg accaaggcgt gcgtgatcgt gccacagcta
     9781 ttgaatttta tgaccaaaaa ggatatgtca caactggaaa gacaatggaa gaattagcag
     9841 aaaaaatcga tttaccaaaa gaaacactag caaaaacagt tgatggatgg aatacggatg
     9901 ttgcggagaa aaaagatgct caattcaacc gtgccacagc catggaacat gacttatcgt
     9961 ctccgaatta ttatgcgatc aaaatcgcac caggaatcca ttacacgatg ggcggtgtga
    10021 aaatcaatac gaacactgaa gtattagata aagaaaacca gccaattagg gggttatatg
    10081 ctgctggtga agtcacaggt ggcttacatg gaaacaaccg gatcggtgga aattcagttg
    10141 cggagatcat tatttttggg cgacaagcgg gcatacaagc agctcgattt gcttcggaaa
    10201 atgaatgaaa tagaacgtaa ataaaaagag gttgtgaaat cagcgttttg agctaatacc
    10261 gagggtcagc tgattgaaca ctgtttagt
//
LOCUS       DACEPW010000090        10212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEPW010000090 DACEPW010000000
VERSION     DACEPW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.091"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            232..1080
                     /locus_tag="IW819_001747"
     CDS             232..1080
                     /locus_tag="IW819_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1244850.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            1094..2167
                     /locus_tag="IW819_001748"
     CDS             1094..2167
                     /locus_tag="IW819_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1244851.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            2157..4328
                     /locus_tag="IW819_001749"
     CDS             2157..4328
                     /locus_tag="IW819_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAQ1244852.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(4440..6611)
                     /gene="copB"
                     /locus_tag="IW819_001750"
     CDS             complement(4440..6611)
                     /gene="copB"
                     /locus_tag="IW819_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1244853.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(6631..8817)
                     /locus_tag="IW819_001751"
     CDS             complement(6631..8817)
                     /locus_tag="IW819_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1244854.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(8817..9026)
                     /gene="copZ"
                     /locus_tag="IW819_001752"
     CDS             complement(8817..9026)
                     /gene="copZ"
                     /locus_tag="IW819_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1244855.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(9039..9479)
                     /locus_tag="IW819_001753"
     CDS             complement(9039..9479)
                     /locus_tag="IW819_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1244856.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9553..10026)
                     /locus_tag="IW819_001754"
     CDS             complement(9553..10026)
                     /locus_tag="IW819_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAQ1244857.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
BASE COUNT         2886 a         2169 c         1829 g         3328 t
ORIGIN      
        1 tactcaattt aatcgtattc tcatttcaaa acgaatgtac tttaaaaaaa cataataaaa
       61 tcactttgct tgtaggatgt attcccttcc attacaatga aataaatagc aattttatga
      121 gaaggtgatg tgaaaatgga agaaaaaatc gaagaatcca aacaaaaaat cgatcagttg
      181 atacaagaat tagaaaaagc ggtcatcggc aaacgttcaa ctatccaatt gatgatcgtg
      241 gccctattag caggtggtca tgtcttattc gaagatattc ccggtgtagg taaaacgtta
      301 ctggtcaaag cactagcaaa agctgtacag ggaaccttca gcagagtcca atgtactcct
      361 gatctgctgc ctagtgatat tcttggtttt tccgtataca attcacaatc aaaagagttt
      421 gaatttcgtc cgggaccgat ctttaccacg atcttgttgg cagatgagat caatcgcacg
      481 actcctcgta ctcagtcagc acttttagaa gcaatggccg agaatcatgc aacgatcgac
      541 aacaatacat accctctaga caatcatttt tttgtcttag ctacgcaaaa tccaatcgag
      601 tatgaaggaa catatccttt gccggaagca cagctcgatc gttttttatt ccgactacaa
      661 atcggttatc cttcttttga tgacgaactt ttgctacttt tagataaatt cgaaaaagtg
      721 ttggataact taaatgaagt attgaacagt tatgaaatag aagaaataaa aacaaatgtg
      781 gcgaatgtct ttgtcagtcc cgaagtggca tcttatgcct tgcagttagt gtcagcaaca
      841 agaagccatg ctgccatcca gcttggcatc agtccacgag gaagcctttc ctttattcag
      901 gcagcaaaag cttatgcttt aatccatggg agaaattacg tcacgcccaa agatttgcag
      961 gatttagttc cttacgtgtt ttcccatcgc ctgatttttt acgatcgttc attgaaggaa
     1021 gacgaaaaaa aacaaatcat tcatactctt gttgcacaaa ttcctgttcc tgtgaggtag
     1081 attgaggtag ataatggaaa aaatcaaaga ttatttcatt aattttgtgt tatttctggt
     1141 atatttcatc attgtgcttt ttgctcttgt attcaacaat tctttaggct ggttcttgtt
     1201 ggcctttttt actctttatg tatcatggag cttgatttca ttaagcagct ttctaaagaa
     1261 actttcggtt acttgtacat tgcagccact cgtttatcgc aaaaaaagta tgcaggttat
     1321 tttttccata gcaaaaaaga gaactttttc tttacctctc cctcgtttac aaatcggctt
     1381 cccagcaatc tttgcagaaa aaaaacaaga gattttgatt cttacaaata aaccgcaaga
     1441 agtccaattg ctttggcagc caaaagaaag aggatgccat gaaacattaa ctgtcatttt
     1501 tacagcctat gatagtctag gacttttcag aaaaagtgca aaaagagaga tctcttttcc
     1561 tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa aaattacaga gagttctgga
     1621 aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat tttcgggaac atcgcgctta
     1681 tcgtccaggt gattctttca accgaatcga ttggaaattg tctgctcata ctcaagagtg
     1741 gttataccgt gaatacgatt atcaagaaga agaatgttct cctctttttt gcttttgggg
     1801 ttcgcacagt ccaaaatttg aacctctgct agacttgtat ttttctttat ggcatttgag
     1861 aaaagaaaag cagccggaac ttctgatatt aggcaaccgc gcctttcatg gcaaagaccc
     1921 cggtcatacc tattttgctt ctttggaagc tggcgatgag aaagcttttt tcgcacacct
     1981 aaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct gagaattctc ctgaagtctc
     2041 agaagctatt gaaacattgt caaaagatcg aacggtttat cttgtttatt tcgtagaaaa
     2101 acaactcatg gtccaagaaa aagacaagat atgttcattg ccaggaggtg aatggattga
     2161 cgattaaatg ggaaaaatgg ctgtgtggcg tttactcctt tgtgatacta agtgtaggtg
     2221 ctccgatatt tgtagtagct aatcaattgc aaaacagcta ccttcttatc ctaactattg
     2281 gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg ggtacgtttg ctgctttctt
     2341 tgccagtaat gataggttgt ttgtttttgt attttccttc gaagacattt tccttattat
     2401 ggatcttaca gtttttttcg tcagtcacag aggaattctt ccatctatcc caaggtaaac
     2461 tttattattt gccagaaaag acagcttttt ttctcataat gttgctgatc tatttattta
     2521 ttacattgac cgtggattac gactatttgt acgcaccctt tctttctttg atggcttact
     2581 tcatgatgct taccgttttt cgggaaaaag atttactgta cgaaatgatt gcggtagtaa
     2641 ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc catgcggttg ttgtcgggaa
     2701 acactcgatt ccagtcattg actacagttc tgcttatttg tttcttaact attagcgcta
     2761 tcttgccttt atatcttccg aaagtacacc aatctctcga ggaaacgagt gaaccgatcc
     2821 gagaatatct taatgatgaa gggctttatg atacactcca tgagtatcaa ttaaccggaa
     2881 gtgcaagaac aggcttcagt gagaatgatg aacagcttgg tggaccttta tatgataatg
     2941 gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta ttggaagatc gaaaataaaa
     3001 atcattacac cggttctgga tggatagaag atcaggacgc acccatccaa ccaatccaag
     3061 aattccctgt tgacatcacg agctaccaaa ccgttatgga agacaaagaa aacattacat
     3121 tgaccctaaa aaaacaagct ttcttacctt atccttatgg aaatactacc ttgactgatt
     3181 tttcattagc tagtggacaa acgctttact atttgcctga aagccagcga ttcattcctg
     3241 tcccgaatga atcgacacgt tctgcaactt tacaaacaga aaggttagtt tttacgagag
     3301 aagaattaaa aagcagtcag ttaaaagctg atgatttaga aaatcagcag acaaatcttc
     3361 aattgccaaa aagtttgcct aaacgtattg aggacttggc ggtcgaattg accaaaaatg
     3421 aagcaacttt aattcgtaaa gtcgaagctg tcgaacagta tttaaaaaca agcggaaatt
     3481 tccgctattc caaaacggat accggacata ctccgaaaga acacgattat gtggattatt
     3541 tcctctttga ttcaaaagtc ggctattgtg acaacttttc aacttctatg gtcgtccttc
     3601 ttcgaacact tggtatccca gctcgctgga caaaaggttt tacaaatggg ctcagaacag
     3661 gcacagataa tcaagggaat gaggaataca gcattctcaa tagtcatgct cattcttggc
     3721 cagaggtata ctttcctgga tttggctggc ttccatttga accaacccct tcatttgcca
     3781 atccaatggg ttcacaacca gttgctgaaa caaccgaaac cgtccagcct gctatcgaac
     3841 aaccaaatga aagcgaaaat atggcagaaa ctatttccag tgagagatct gaggtcattc
     3901 catcagaaga aacaacactc acaccagctt ataaaatcgt cttattcagc cttggggtgt
     3961 gtttatacgt cttgttgttc tttctattgt tattcagaaa atctctgcgc tggacaagtt
     4021 atcttatcta tcttcatttt tttgcttcta ctaatacgat tgcatatcaa aaacttctta
     4081 accatttgga aacattggta ccgcgcaaac ccgcagaatc tttgcaggaa tatagcctgc
     4141 gaatagaaca agaattcaag ctagatctgc gtttcattcg attaactact caatatgaag
     4201 cagaaatata tggacgacaa gcagatccgg taaatttcga taaaaaaacc tatttagaaa
     4261 ttacgcatat aataagaaaa acggtataca aaaaagttct gacaaaaggg agccgcttca
     4321 aaaaataaga taaaatttct ggagaatcta cttctggaat gccgagaacc agctgcttga
     4381 gtatcgatta ttcgaaaaaa aggttgtgac acgttttttg tcacaacctt ttttcattat
     4441 tatttgattt tcaatgtcaa agcatttaga gctacaacta ccgtacttaa cgacatcaag
     4501 acagcaccaa ctgctggact taatatgata ccaattggcg caagaattcc ggctgctagc
     4561 ggaattgcaa gaatattgta acctgccccc caccagaggt tctggatcat tttctttcgc
     4621 gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg gatcgctgtt tgtcaatacg
     4681 acatccgcag aatcgattgc gatatctgtc cctgcaccga ttgccatccc gattgaagca
     4741 cgggctaaac ttggcgcatc attgatccca tcacccacca tgattacttt tttcccttgg
     4801 tcaaggtatt tctggacaac agcttcttta tcatctggta ataaaccgcc ataatattct
     4861 tcgatcccta aataatctgc tactgcttgg gcagcgtttt tattgtcacc agtaagcatg
     4921 acaggagtga tttttctttc tttcaatgtt cggataaatt cttttgcttc tgtttttact
     4981 acgtcaccta aagcaacgat tgcagctaga tgattttcta aaatcaggaa gctgatggta
     5041 tttccttgtt cttggtaagg tttcaataat gcttgttcta cgtttaatcc gagacgctca
     5101 acttcttttt cattaactat tttcacttgt tggtttttga ctgttgcctc tagcccaact
     5161 cctgaaagat tttttagatt ttcggcttga taaggcttga tttcatgttc tttcaaataa
     5221 ttcataatac caacagccaa tggatgattg gcattcgctt ccaatgctcc taaatattga
     5281 aggatttcat ttttattgaa cgcatcatca agtacttcga cacccgttac agtaaatttt
     5341 ccttctgtta gtgttcctgt tttgtccagc atgatcacat ctagatcatt ggcttgctcc
     5401 agtgcattgc ggtttttcaa aagtaatccg tttttagctg ctattgatgt agagcgagca
     5461 acaactaatg ggatagctaa acctagagca tgaggacagg cgatgataaa gactgttacc
     5521 atacgttcta atgcattagg aagatctgtc aagaacaacc aggcgatgaa tgccaggata
     5581 cccacaatta atgcgacata aaacagccat tttgccactt tgtcagataa tgactctagc
     5641 ttggattttt ctccttgtgc ttttcgcacc atttccataa ccttcgctag ataaccattt
     5701 tcaccagttc cagttacttc gatttcgatc gttccatccc cattgataga accgccaatc
     5761 actttgtcat tggcttgttt tttgattcct cttgattcgc cagtcacagc tgattcgtca
     5821 acaattgttt cacctttcaa gattacacca tctgttggca ttttatcacc agaccggacg
     5881 atcaaacgat caccttcgtt tacttctttc aatgaaactg tctcttcttt tccatctttc
     5941 gtcaagcgtt ttactgattc tggcagtaat tctgctagtt tttgtaaagc attgctggca
     6001 ttcgacacag cattcatttc aatccagtga cctagtagca taataacgat caaagtagcc
     6061 aattcccaga agaaatccat gacgtgtgta tgtggattga gcagatttgc gataaacgaa
     6121 tagacactat aaatatatgc aactgtaatc cccatcgcga tcagtgtcat catagcagga
     6181 cttttttgtt tcaattccat cttagctcca cttaaaaatg gctgaccgcc atagataaat
     6241 agtatagttg ctaagatcaa tacgagccaa tcagaaccag gaaacgttac ttgaaatgga
     6301 aattccattc ccatcattgg tgaaaaaaga atgattggga tagctaggat aagcgatagc
     6361 caaaattttt gtttgaaatt tcccatgtgc atggaatgat ccatgccgcc atgatcgtga
     6421 tccatgtgca tcgaatggtc catttgtttg ctgtttgcta tatctccatg gttatgtccc
     6481 atgtgcatgg aatggtccat ctggctatgg tcatgtttca tctccatctg tttttcatga
     6541 gattctgtac tttctttgtt tactacttct tttttatgtt catgtttttt ctccatatcg
     6601 ttcccattca tttttactcc atcccttctc ttactttttc atgtgccgat ttaagctcag
     6661 ggagttcaac aatacactga cggaactaaa cgccatcgct cctcctgcaa tgattggatt
     6721 caaaaagcct agtgcggcaa aaggaatacc aatcgtatta tagacaaaag cccagaaaag
     6781 attttgtttg atttttctca atgtcaccct agacaactcg attgtttgtg caatggatgc
     6841 caaactgcta ctcatcagcg tcacatcggc tgtttccatc gcgatatccg ttccacttcc
     6901 cattgccata ccaacatcag ctaatgctaa agccggtgca tcattgattc catcgcctgc
     6961 cataccgact tttttgccat ctttctgaag tttttctacg taggctgctt tatcctctgg
     7021 aagtacttca gcaaagatat gctttggatc aataccaact tgtttaccaa tcgtttcggc
     7081 tgccagtttg ttgtctcctg tcagcataaa aacatctaat cctttattct ggagctgctt
     7141 gattgcttgt ttggcttcta gtttgatttg atcagccacg gcaatcaacc cgatcacttg
     7201 cttttcatca gctaaaaaca tgactgtttt tccctgctgt tctaattcta acgcgtgttc
     7261 ttggtattca tcaaatgata ggttcaattc aattaatcgt ttacgtgtac ccgcaaaata
     7321 gcgtactccg ttaatcgtac cgctgattcc agcacctggg tgagctgtaa aatcaatcat
     7381 atcgtatgtt gctgcttctt gcagttttcc gtattccacg attgctttac ctagtggatg
     7441 ttcagaagct tgttctaaag tataaaaaat agataggact tctttagaac cgactagatc
     7501 tgtaactttg ggctttcctt ctgtaatcgt cccagtttta tccaaaacaa tgctgtctaa
     7561 gtgagcagct gcttctaatg cttctccgcc tttaattaaa attccatttc gtgcaccaac
     7621 acccgttcct accataatag ctgttggagt cgctaaacct aatgcacatg gacacgcaat
     7681 gactaataca gagacactat ggatcaatgc gagttgccaa tcaccagtga tcaaacctgt
     7741 agctatgagc gtgataaacg cgatgactaa tacgatcgga acaaaaatcc ctgagatccg
     7801 atctgcgatt ttttggattg gggctttgct cccttgagca tcttccacca tttgaatgat
     7861 ttgcgctaat actgtctgct tgccgacttg tgtgacttcc atgtgtagca gaccattcgt
     7921 attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt tctacaggaa gattttcacc
     7981 agtaagcata ctttcatcta tcgcactgct tcctgaaata attcgaccat ctgctggaat
     8041 ctgctcacca ggacggatga ctagttgatc accaacgact acttcttcaa tcgctaacgt
     8101 ttcttctttc ccgtttctta tcacttgggc ggtttttgtt tgaagagaca tcaattgttt
     8161 gattgcatta cctgttttcg tttttgctgt atgttccaaa tatttgccga gcaaaatcag
     8221 cgtgatgatc atactgctgc tttcaaaata aagatcttgg gggtgtccac ggaaaaatcc
     8281 gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt gctaccaata catccatatt
     8341 tggtgctttc gacttgattg catggtacgc acctttataa aaacgagcac cgacataaaa
     8401 ttggacagga gcagacagca ctaactggac gattggaaaa tgaaaaaagt gaacaatcgc
     8461 cgcatggctt cctaacatca tagcaatcat tgacaacatc aacggcaaag tcaaaaccgt
     8521 actcaatata agatcgaata gcattttttt caaatacgct tgcttttctt ctgcaatttt
     8581 ttgtttgtgg gcttcatcgt acaaaattgc accatagccg atgttttcta cgctttggat
     8641 taatttttca gtggtcgtcc cttcatattt gacacttgct ttttcagtgg ccaagttgac
     8701 tgtggcattg accacaccag gttgtccgtt cagctctttt tcgatcctag cagaacaatt
     8761 tgcacaagtc atacctgtaa tgacgaacgt atccattttg gtttctgctg ttgccatcaa
     8821 atcacctctg ctttataacc caatccattg attgcttgac aaatcttttc ggattgaact
     8881 tttgtttcat caaacttgac cgtaccgttt gcttttttca aatttacttt cactttttgc
     8941 acgccgtcaa gtgcgcttac tgtttcttct actcttgctg cacaatgatt gcagctcata
     9001 ccttcgattg aaaattgttg tttcattttt tcttcctcct attgatgttg tttacattca
     9061 cattgacctg ggatacagtt acattcgatc gtatctactg gttgcttttg ttccagttgt
     9121 ttcaaaatct cattgatatc ttcttgagtc aaagtggctt cttctactag atccgcaatc
     9181 gtctctccaa tcctttttgc acagatatgg gaaaaaagat tttccgtcgc actgcgaact
     9241 gtttccatct ccgagacagc tggatgataa atgaattttt ttccttcttg ttctgtccag
     9301 atcacttctt tttttaccaa acgacccagc aaagttttga tcgttgctgg tttccaatcc
     9361 atcgaatcag ctaagatctg cgtgatttct tgggctgtcg tctgtccaag tgtccaaata
     9421 acacgcatga tctcccactc tgaatcacta atctttactg gcgttttgac tttttccatt
     9481 tatttcacac ctttcgttta caaatgtaat cattaactta tgaatacaga ttacacttgt
     9541 aaacaaaaac tgtcaattat ttttgctctc tattttcaat ttagttaaaa attcttgttt
     9601 ctctcgtctt gttttaagga tgatcagttt atttcttgca ttagctactt ggatcagctg
     9661 ctgtattttt ggttcattgt ttccttcaaa atgccagata aaagataaaa attctaagta
     9721 tttcctgttt agtttctccg aaaatgattc aggcatatct ggccgagatt ttttgtttat
     9781 ctttcttttt agagaacgcc aaactactcg gtagatggcg agataacgcg gcactttaag
     9841 ccaaatgatc ttatctgctt ctttaatcct ttcctctaac gtagaagtat aatttccttc
     9901 aacgatccat gacggattag ataccataaa cgattgctgt tcttgcaaaa accacttttt
     9961 tgcctgaatg cggaaagccg gtgatttctt tgatcttcct tgcgagggac gattttcccg
    10021 acccactttg tccaatcacg atgatcttca ttcttctccc tcacttctct ttggaatttg
    10081 gttcgatcat accacttata acgccgcaaa aaaacaaggg actgtgacaa aagtcgtgtc
    10141 aaagtccctt gtttcgaata aatggtgttc aatcagctga ccttcagcat tagctcaaaa
    10201 aacggcaaaa ta
//
LOCUS       DACEPW010000091        10151 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEPW010000091 DACEPW010000000
VERSION     DACEPW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.092"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(323..1105)
                     /locus_tag="IW819_001755"
     CDS             complement(323..1105)
                     /locus_tag="IW819_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244858.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1170..2006)
                     /locus_tag="IW819_001756"
     CDS             complement(1170..2006)
                     /locus_tag="IW819_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1244859.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2024..3433)
                     /locus_tag="IW819_001757"
     CDS             complement(2024..3433)
                     /locus_tag="IW819_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1244860.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3447..5270)
                     /locus_tag="IW819_001758"
     CDS             complement(3447..5270)
                     /locus_tag="IW819_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1244861.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6113..6439)
                     /locus_tag="IW819_001759"
     CDS             complement(6113..6439)
                     /locus_tag="IW819_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244862.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6485..6847)
                     /locus_tag="IW819_001760"
     CDS             complement(6485..6847)
                     /locus_tag="IW819_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1244863.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6884..7099)
                     /locus_tag="IW819_001761"
     CDS             complement(6884..7099)
                     /locus_tag="IW819_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1244864.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7226..7561
                     /locus_tag="IW819_001762"
     CDS             7226..7561
                     /locus_tag="IW819_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1244865.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDVVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7637..9634)
                     /locus_tag="IW819_001763"
     CDS             complement(7637..9634)
                     /locus_tag="IW819_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244866.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9732..10007)
                     /locus_tag="IW819_001764"
     CDS             complement(9732..10007)
                     /locus_tag="IW819_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1244867.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT         3146 a         2112 c         1493 g         3400 t
ORIGIN      
        1 ttatctttag aaccatttca gatacatatg aaacaattac atatgatttt ttcataaaaa
       61 aacagtactg tctagtagta taaaatatac aaatttctta aaaatttttt cactaaaatc
      121 actacctgaa acagtctaaa acacttatat atcaacgttt aatgaaacta tctccgtttc
      181 atttcagata caaataaata tagtcgggct ccaaatccga aaaaaaatac ccccagtagc
      241 ttatcctaag ctactggaga tgtataagta cggtcatata acaacggggt agggtaaata
      301 aatgaaaaag atggaagaat cattattcaa taactttttt aaatacttcg ttgtaatcag
      361 ttacctgtcc gttcttaaat accacagaat aatctttttt cccattttca agtaaccgaa
      421 gtggaatgtc tttaccaact tgctctgtca ctgcattact gatacctgtt agattagcta
      481 ataaatctga taaagattga gcatctgtag aaatctctgt atcagtattt atcgtgattc
      541 ctttatcttc atttacaagc ttccaagtag gtggtatagc tgtgtatgtt tcgatgaatg
      601 aatcaattct ttcatctatc gtcttcccag tatctacttc tgtctcatta ctcttagatg
      661 aatcgattct ttcatccatc gtctgctccg taactgcttc tgtctcatta ttctcaccgg
      721 aaactctctt cacacccaaa cttatatatt gtccatcttt agtgtgcata gttaaaattc
      781 ccacgctttt actaataaat tcactataac cagttctttc aatagagact tcaatgtatt
      841 tcacactatc atactcaaaa gaaaaataga catgtaaaag cattttgttg tttacatcta
      901 cataataatc ctttgactct acttgagacc ttcctatacg gaatacagct ttactgtctg
      961 atatctctat accagcatcc ttattattaa tctgccaatc tccaagtagc tcttctggca
     1021 agctagtagt ttcttgtgaa cactttacca cttcactggt agaggtcgaa acattcacct
     1081 ccgcactgtt agaagaattt tccaaggaat caggagcctt agcacaacca acaagtcttt
     1141 atatattgtg cagccgatat tttcttcatt cagtacaccc ttgaaatatg cagtgttaga
     1201 taaagcagtt cgtcctgatt tgctgaggca cccatctctt cttgaacgta ctccttgatt
     1261 ttcaaggccg cccgatactc tttcggaaaa cgctgcttca ctgaagccag caaaaaatca
     1321 tccttctccg gcaactcttc cttcttaatc attcgggcaa taaaaaactg taaatgcgtc
     1381 acaaatctta agtaaagcat cgaatcttta tcaacctttt tttcgggaaa cagatatcct
     1441 agcatagcca agatatcctt tagtgctctt accgagagca tggtttcttc catatttgct
     1501 tgttcacttt gggcatttac aatgtgaaac gcaatgtttc cagcctcctc ttctggcaac
     1561 tgaacattca tttttttgtt gatcaattgg atagcatagc agccgatctc aaattctttt
     1621 gggtaatact tttgaatttc aatcagcagg cgattttgaa aaacaatgcc tttttgactg
     1681 cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa atagactttt attcagctta
     1741 aagtcaaact tggcgttggc ataatcgata attcgcttga cgcaaataat tagatcatcc
     1801 ggagcagcct caaacagttt aatgtaatcc ttttttagag catcagaaag tgaatctaat
     1861 ttaaatactt tttggatttt gtcagccgga accaaatcgc ctttctttag ttgaaatcca
     1921 atccctttcc ccatcaaaat tacttctttg ccttgcttat ctaagcaaag aattaaactg
     1981 ttgtttaacg ttttaattac tctcattaga ttcaccgttc tctctaagct aagccatttc
     2041 ttgcgattac tttttgatac caaaagaagc tttctttttt tattcgtttt aattctttaa
     2101 gattctcatc ggttcggtca acatatataa aaccataacg cttattgaag ccttgatggg
     2161 tactaacaac atcaatcgct gaccaaggta aataaccaaa aacatctaca ccatctgtta
     2221 aagctgcttg aatagccaga atatgtttgg ctagataatc aattcgatcc tggtcattaa
     2281 ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc attttcagta attaaaatag
     2341 gtaaatcata gcgatcgtac aatttcctca atgttaatcg tagtccagta gggtcaacta
     2401 cccagccata tggcgtttta tctacataag gattttcagc tgcccggtag acaccttctt
     2461 ctcccaacat gatctgttga tcccccgctc tagcggaaac atctgatccg tcatttttgc
     2521 tggcagcgat ggtggcagtt gaataataat taatcgcaat aaaatcgggt ttaccggagg
     2581 ataaaattgc ctgatcctct gcctcaattt taggatataa tccacgatca ttcaaatagt
     2641 tttcaaacaa ataattgtat tttccccgaa ctgctacatc taagaaagac cagcctctca
     2701 atgtttccca attatgggct gcaattgcat cttctggact ggaggtagct tgatacatag
     2761 ccgttaaatt gattgctgga ccgatttttc ctttaagatt caactgatga tacaagtgaa
     2821 ttactctagc ttgagctaat aaaacatggt gattaatttg aaagagttct ttttttgaag
     2881 gcagctctcc acttggtggc aaaccaatcg ctccaggatg aaggatcatc gtgttttgct
     2941 cattaattgt cagccaatag ttcaccttgt caccatactc agtaagcaat acctgtacat
     3001 agtccacaaa ataatcgata gttgctcgat ttaaccaacc gccctgctcc tctagaaaat
     3061 aaggtaaatc gaaatggtac atggtgacaa tcggttcgat tccctgattc aggcattcat
     3121 caattacttg atgataaaaa gaaattccct tttgattggt tttattattt tcaatatcca
     3181 taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa gcctaattct ttaaacagct
     3241 gtatatcttc tttatagtga tggtaaaaat ccgaggcaac ttcaaaatta gctacccctt
     3301 ctgtatgctg atacttgtca ataatagacg gttgtttccc atcctcctga taggcaccct
     3361 ctgcctggta ggcactaatg gaggcacccc ataaaaactt ctctggaaat gctgaaagct
     3421 gtttgtattg cacaaaaatc ctctccttaa ctaatttgca tcaatggttc gtgttccttt
     3481 aagcttccgc ttgcttcact aattcctgcg taattttcac tgttggtcac taccacaatc
     3541 actgtatcat cgtagccagc gtttctaatg gcagcgaaat cggcgttgat taataattgt
     3601 cctgctgaca cctgatctcc tgcttttaca aatacttcaa agggttcccc ttttaaattc
     3661 acggtgtcga tccccacatg gatcaataat tcaatacctt ctttagaacg tagaccaatc
     3721 gcatgtttag attctaaaat agtaatgact tctccctcaa caggtgaata aatgctgttt
     3781 tcttctggat gaatggctac gccctttccc aatagtccct tagaaaagac agcatcgttc
     3841 acttcagaaa taggacacac ggttcctttt accggatttt caacaatgac tgcttcggct
     3901 attgccgttt gctctgattc atcagaagta tcgtcaaatc caattagata agcaatgata
     3961 aaggttgcaa caaaggcgat tacacacgta ataattgcgt gaacaatatt cattggattt
     4021 tctccaataa aggctggtaa ggctgctaag ccaggggaaa caaaggcata tctgactaaa
     4081 ccgctcaagc cagcatacaa accagcaatt gcaccaccaa tcatggaagc aactaatggt
     4141 tttttcagct tcaaggttac tccatagagt gaaggctcag taattcccat taaagcagtg
     4201 atcccagtgg acacagctag ctgcttcaac tctttgtttt tagttttcgc ggctacagca
     4261 aaggtagcac ctgattgagc aatattagaa gccagcattc ctggcccatt gatcatttca
     4321 gaacccgtac tgctcaattg catagtagcg attggcgtca ttgcccaagc tgtacccgta
     4381 accaccaaaa atggctgcaa accacccatc aaaaatggaa ttaaccaagc tgctttgccg
     4441 ttaatccatt cagcccccat tgctataaga tcgttcagca acgttccaaa cggtccaatg
     4501 acaaccaacg ctaaaggccc cataattaaa aataccagca tcggcttagt aaagaattta
     4561 ataaacgaag gcgaaattcg atccgcaaag ggctctacat aggacatgat ccaaacagta
     4621 aagataatcg gtaaaactga tcccgcataa tctgcagcta ataccggaat tcctagaaaa
     4681 gaaacggcat cgccagtaga aaaaagctgt gaaatatttg gatgaagcaa aatgccagca
     4741 acagtaatcg ccaaaattgg gttgacatca aactttatgg ctgccccata ggctaatagt
     4801 actggcataa aataaaaggc tgcatcagca atagtattca aaatgatgta agtttgacta
     4861 ctgtctgata gtacaccagt caaagtcaag acggccaaaa tggcttttac catcccggca
     4921 cctgttatag ctggaatcat cggtgtaaag gtggtagaaa tgacactaat caatcttgaa
     4981 ataatacttt cttgattgtc tgaagagggg ccctttttaa ttgaagttcc cacttccttt
     5041 tgaatttcct tgaaggctgt ttggacttca ttgccaatca ccacttggta ttgtccaccg
     5101 ctatccacca cactaattac accaagtgtt ttttctaagg ctttcgtatc ggctttcctt
     5161 ttatctttga gttcaaaacg cagtctggtg gcacaatggg ttaagctgac cacattttct
     5221 tttcccccaa cctgttgcaa tacatttctt cctacttctc tgtaatccat tattctctct
     5281 ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca atcaacattg aagtgttagt
     5341 ttgtcttggt cttgcctgca taatcagtaa catgccaata acgttatttt gctgtaatcc
     5401 aataatagca cattatgata gcgcaatcaa cagggaattt aattttttat cttttcgttt
     5461 gaccgtatag ggtaatataa cagttagatc acatcaacta gggtatatct gcgtatactt
     5521 actgctaaaa taagtatatc taatattaac tataccagta aaaccattcc tacataacca
     5581 gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat ataacggatc acacaacttc
     5641 aacaattctg ctcttgggaa aaattgatga atttggtgag taaaggatgg aataaacagc
     5701 attccaacaa tataggcaac aatagtgata cttgtttttt tggtaaaaat cagtaagcaa
     5761 tagccaattt caatcattcc gagatataaa ggtatcgata gaatgatttg ggctagtatt
     5821 cttaaaaatt aactagcaaa tgtttaaact gatttatttc taatctacat gtagtaagta
     5881 tttcaattcc accaattatc aataaaaata ccaaacatat ccataataat tttttccttt
     5941 tcatttcttt tatattgtta taacagtgaa taacataaaa attcccgaca atcattgtaa
     6001 tatttttttg tcacctgtat tagtatttaa tttataggaa actataaaaa ctccctagtg
     6061 aatttacacc tttgggagtt cctgtttgga cggtatgcat aatacattta aatcaattta
     6121 acattaacac ctcagcatat ggtatctcca catatatacg gtctttcttt ggatagtact
     6181 caatatttat gttgatagca cttccattgt ctaatatagc tttagcccgg tatgtgttat
     6241 ctccattttc atgagtaatc gtcacatcat cacatgatcg atttaagttg tactgttctt
     6301 gtaaaatctg tgttaccaat ggttttgatg acttctctaa tccagatgta cctgtcattt
     6361 ttgaaaaaat aatacctata atagccaaga caacaatacc tataatgaca aacttcttgt
     6421 tcatatttac tttattcaac tttttatctc ctatctccaa aataatttgc tcttatccct
     6481 cttatcagta cattgacatc cctaaaagta caccaaagat tgctccagct acccctgcta
     6541 tcatcaaaat cgtacccatt ttctcatctt tttctcgata taagtagccc ataataacag
     6601 caacagcacc aaatacaaca ggaataaatg ctaacgatac taccatagaa atccaaccaa
     6661 taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc atttcattaa
     6721 ttgtctgctc agtacttttc tttactgtgt ctgttccggt tgtctcatct gtttcttgta
     6781 gtgctccaca aaactggcaa tacttacttt catctgttat ttctttgcca cattttctac
     6841 aatacatgtc ttctcctcct aaaatcattc aattgcttat tagttaactg tttactttat
     6901 taaatacatc agaaaacagc tcattcattg gtacacttaa tatctcagaa agcatcttca
     6961 tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat tgctgtgtaa
     7021 cattcatttt ttttgctaac tccgtttgag aaagtccttt ttcttttctg tatcggctga
     7081 gattacttac ttttttcatg attctcccct cctgttttat tatatacaac aatatgatgt
     7141 atttcaactc ataaaatata aaaaacttca aataccatta caaatacaac aacttgttgt
     7201 actatactga gatgggggtg tattaatgaa acagaaacaa tatgcaaagg tcattagaga
     7261 aaagcgaaaa gctctcgggt ggacacaaaa agaattagct gaaaaaatct tctctacaca
     7321 acaggcagta gcaagatggg aaaattctgt aacagagcct aacctcgata gcttaacagc
     7381 tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg tcgtagatta
     7441 tgaggaagag tttttggctc tctatcgttc tcttagcact gaagatgtag ttcgaacaat
     7501 tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat tgaaagattg
     7561 aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat aagattaaga
     7621 gctatggata acctgtctag tttaatacaa ttgggtcttt tctcttcctt actctccgtc
     7681 catcatctct tgttacactt gtaaattgat acagaggaac tatcccagct cctgataata
     7741 tcaattcttt tattgtttca tctatccctg cccctttcgc taaatatatt aaaaaactac
     7801 tacttgcttt cacattgtat agtttcttaa tatccttaga agctcgttca agagtgaatc
     7861 cttgtttaac aaagtagcct ctgtttttta ttaaagcatt tttcacgctg ttatccccaa
     7921 cattacgtag gcgttcacaa acagtgttaa taagttcttt ctctgtcatt agatatgtcc
     7981 cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat tgtttttcct
     8041 tgctgagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt tcgttaaaac
     8101 atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaattttt tccagaagtg
     8161 gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg tctaatcgat
     8221 agacattgct tctcttatga ttaaagtttc ctaacgcttt ctcttccttt aacctattta
     8281 ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc aatcctataa
     8341 aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta ccatagtaag
     8401 aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt tccttactat
     8461 catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt tcctctagat
     8521 aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca aaaagtactt
     8581 ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc tctaatagcc
     8641 aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact ccaataaaat
     8701 tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca gttttactgg
     8761 taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag tttttacttg
     8821 acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca ttaaaagaca
     8881 tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct ttgtcgtcat
     8941 tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc cctgaatcac
     9001 tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta atatctaaca
     9061 tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa aacattctat
     9121 tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat tcgtaaacag
     9181 caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc ttgtattttt
     9241 ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta tgaactaaat
     9301 tgatagataa aaccttttct atgcttaagt atctactatc atctttactc aaatctacta
     9361 ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact ctggtttcca
     9421 caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat gattgtgatg
     9481 aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct aaacacctat
     9541 ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt tcttgatcta
     9601 cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta taaaattctg
     9661 ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat gttttagtct
     9721 tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga ttttttaaat
     9781 ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata ttttctaatc
     9841 gttcctactg atacacctag atattcactt gcctgctgat agtccatata ctctagagtg
     9901 actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc tttcacttgt
     9961 tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt tccccctcct
    10021 ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc tcaaaataaa
    10081 agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca tcgacataca
    10141 cataatacct c
//
LOCUS       DACEPW010000092        10142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEPW010000092 DACEPW010000000
VERSION     DACEPW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.093"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(115..1506)
                     /locus_tag="IW819_001765"
     CDS             complement(115..1506)
                     /locus_tag="IW819_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1244868.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1719..2105)
                     /locus_tag="IW819_001766"
     CDS             complement(1719..2105)
                     /locus_tag="IW819_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1244869.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2161..2784)
                     /locus_tag="IW819_001767"
     CDS             complement(2161..2784)
                     /locus_tag="IW819_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1244870.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2848..3516)
                     /locus_tag="IW819_001768"
     CDS             complement(2848..3516)
                     /locus_tag="IW819_001768"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1244871.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3528..3968)
                     /locus_tag="IW819_001769"
     CDS             complement(3528..3968)
                     /locus_tag="IW819_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244872.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4027..4698)
                     /locus_tag="IW819_001770"
     CDS             complement(4027..4698)
                     /locus_tag="IW819_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1244873.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4883..5281)
                     /locus_tag="IW819_001771"
     CDS             complement(4883..5281)
                     /locus_tag="IW819_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1244874.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5278..6051)
                     /locus_tag="IW819_001772"
     CDS             complement(5278..6051)
                     /locus_tag="IW819_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1244875.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6048..6731)
                     /locus_tag="IW819_001773"
     CDS             complement(6048..6731)
                     /locus_tag="IW819_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1244876.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6898..7785)
                     /locus_tag="IW819_001774"
     CDS             complement(6898..7785)
                     /locus_tag="IW819_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1244877.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7782..8651)
                     /locus_tag="IW819_001775"
     CDS             complement(7782..8651)
                     /locus_tag="IW819_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1244878.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8788..10074)
                     /locus_tag="IW819_001776"
     CDS             complement(8788..10074)
                     /locus_tag="IW819_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1244879.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         3036 a         2214 c         1681 g         3211 t
ORIGIN      
        1 cagaagtagc ttctcggaaa taagccgaaa tttcacaaaa atttgagaaa ctatttttag
       61 gaaacttctt cttatttctt aaagctgacc acttctatca caacctcttt ttgactatgc
      121 attcacatcg atcactttag aatcatcatt gatgacatcg cgatctaaat ctttgatgcg
      181 tgcacttgtc acgataccag ctaccatgct accgctgacg tttactgctg tacgtgccat
      241 atcgattaat ggttcaacag agataactaa cccaacgatt gctactggca agttcattgc
      301 ccctaaaacg atcagtgaag caaatgttgc gcccccaccg actccagcta caccaaaaga
      361 actgattgtt acaattgcca caagcatcaa aataaattcc aaactaaaga cattcactcc
      421 agctgctggt gcgacgatcg ttgccaacat agctggataa acgccagcac agccgttttg
      481 tccaatagac aaaccaaaac ttgctgagaa attagctgtt gcatcatcta ctcctaaagc
      541 ttttgtctga gtctcgatat tcaatggcaa agctccagca cttgaacgtg aagtaaacgc
      601 aaagctcaat accggaaagg cttttttgaa atagttgatt ggattgactt ttgcaccaat
      661 caaaatcagt gagtggacaa tcaaaacagc aagcaatgct acgtaagaag caacgacaaa
      721 tttacctaaa ttgacgatag ctgcaaaatc acttgttgcc aaaacgtttg tcatcaaagc
      781 aaatactcca taaggtgtca aacgcaaaac aagcgtcacg atccgcataa tgattttata
      841 cagactgtcg actaaatctg aaaagaatgc tgcttctttt ggcgcttttc ttttcacgcc
      901 tagataagcg attccgacaa atgccgcgaa aataacgact ccaatcgtac ttgttgcacg
      961 actacctgct aaatcagcaa aaacgttttt agggataaag gataaaatct gctgtggaat
     1021 cgataagttt tcaactgttt cctgacgtgt tgccagttca gagattcgtg ctgtttctgc
     1081 agctccttgt gtgaatttag ctccttggag tccgaacagc attgttgaac caattccgat
     1141 caatgctgca atagctgttg ttccaagtaa agaagtcaat acagtcgcac taattttccc
     1201 tagcttttca gaaactttca tttttgtgaa tgcaccaatg atcgaaataa acactaaagg
     1261 cataactagc atttgcaaga atgatacata tccttgacct acaatcccga tccattccat
     1321 tgattgagta acgactttgt cgtctacacc aaagatcaat tgaagcagac cgccaaagac
     1381 gataccaact cctaatgctg taaagacgcg tgttgaaaat tttgtatggc gcttttgcat
     1441 acgatagaac acatataata atgccgcaaa aactagaacc actaaaactg tgatgagtgt
     1501 tgtcattttt ctcctcctca tattattaat gacaagtcca tcgaaggaag taaaggttcc
     1561 cgagaatgat tcttcaacca tcctcctgaa aaaatttttt tcagtgactt ttatatttta
     1621 gacgattttt atcgaaatga cattcttttt gcttacaatt agtaataaaa aaacatacct
     1681 gtgatagctt taatctatcg caggtatgtt tttactgtct attcttgttt ttctgattga
     1741 aaattcaatt ttaccccagg ctgatctaag gcgatctctg tcacctcata agacattcgg
     1801 cgtacgatat cgaataacgg tgctactagc tcgtccactt cttcttgtgt gacatcattt
     1861 tgaccttcaa ttttccgatt gaggatatga ttgatctgac tcacacttcc agataaaaca
     1921 tatgtatcaa acactaaacg gaactctaag cgtgctccta cgatgctatg ttctttcgga
     1981 tagttttcga tcgtttcttt taaaggcgag aagcctactt tcaaatctgt ttctacttct
     2041 tggttcggca atctcatatc ataatgaaat gcttctacaa cttctttttg tcttctgatt
     2101 tccatataat tttatcactc ctttatgaaa attataacat atctcctttg aaaggggata
     2161 ctacttttta atgatgctct gcaaggtaag gatttcattc agatttccta ccacataatc
     2221 tgccgggatc tcacccagaa aatgatcgat gtcgtaaaga caagtagcga ctcctgcatt
     2281 tttccctgct tcgatatcta aacgacgatc gccgatcatc atcgtgtctg ttctttctaa
     2341 atgaaaagta tcgatcaaat aatttaaaga tgctggatca ggtttacgag gaaagtcctg
     2401 atcgatccct actacttcct caatcagatg tgccaatcga tgttctttta acaggcccca
     2461 tgttgattct gtcaaacggt gggtcaggat gaaatgacga ccgccgttgt cttttagcat
     2521 ttccagtact tgtttcgttt ctggaaatgg acgggaaact tccgtacttt ctttttcata
     2581 tcggtgaaat aattcattaa actctctggc attcaagttt gattcattga tcaaataact
     2641 agtagaatac tttttcattt tgaagtagat ttcttttttg tccatactga taccaaaatc
     2701 ttttagtgct tgccatgcac catctaccat tgcgggatac gtatcaaaca atgtaccatc
     2761 aaagtcccat atataatttt tcatgttcct tttgttccat tcctttgctt cttttttcta
     2821 tctctcactt ttagataaag gtgttcttta aagtattttt ttgtccagaa ttgtcaaatc
     2881 atctgctaat gtatactgga ttggttgtgc atttgggata tcgatcgtat ccatttgatc
     2941 ttcaggaaca tcttctaaat attttgtcag tgctcggatg ctattaccat gacccacaac
     3001 tagtaaatct tgccgatctt ttagttttgg tgctagatga tcttgccata agggaatgat
     3061 ccgattcact gtcatttcca gactttctcc gtatgggatc aaatgcggat ccaagtgatc
     3121 atatctgcgg tcaaatctat tttctgcggt taacggcgga acttccgtat atcctctgcg
     3181 ccatgttttt acttgatctt ttccgtatat ttctttcatc ttatctttgt ttttacccac
     3241 aagatcccca taatgccgtt catttagacg ccatgttttt attataggaa gccataactg
     3301 acccatttct tctagtgcga cttgtccggt aagaatcgca cgtttcaaaa cagaggtata
     3361 aacggcatcg aattgaagtc cggcttcaag cattttttga ccggcaactg ctgcttgttc
     3421 ttttcctttt tctgttaagg cgacatctag ccatcctgtc cagtaatttt caaaatttgc
     3481 ttcgctttcg ccatgacgta ataatactaa tttcattctt ttctccctta aacattcgtt
     3541 tcgaatttta taccacatgt tttgcatgtc acacgtattc gtccttttcc tttaggtgca
     3601 cgctgctttt gatgacaatt cgggcactca aaaaaagtat aactttttcg atccattttg
     3661 acttgctttt tgttttttat ctgatgatat ttttctttga ctctatcaag atatttcaaa
     3721 taacgttgat tctcatttag tctggggtag attttttttg ataggaaacg aaatatcatc
     3781 catccgaaaa agacgatgaa caacaattgt ccgattctat agggcaacca acgacttagc
     3841 aaactaaaga caagcgcagc gatcaataga ccgcgattca actgatcaaa tttcgcatat
     3901 cttccctgca taagtttgtg atagttctgc tgaaatcgta ttagcctttc taaccatgtt
     3961 tgcatcatat tatctcctcc atcatctaat atttattatg acaaaaaaat ccaaaaagaa
     4021 aaagccttaa ttggcctttt ccattgcaac ttggtgaagg acattccgat ttctggtaaa
     4081 gtcaatcaca atcactttct ttttatcttc tctgttgact actcgaaatg tgtccgtcaa
     4141 tggaagaaac agcttcttag atggtagctg ttgcatacgt ataaaacgat ggatcatcgt
     4201 aatcaattga ggaaattgaa tagacgtgtg aagcagacct ggatattttt tcaacagctg
     4261 tgtcgttttg atcagattcc gttttttact gatcactttt aaaaagcttt ttagaatctt
     4321 ttgctgacgt ctaaaaatag aaaaagaatt ttcgtcatct atttgataac gcatttgttt
     4381 ttgcagttct gctgcagtca gatggatctt cccatcgttt gtatcgatca ttaaaccttc
     4441 tggaaacagt tcaaccactg ctcgatccca agattcaaga gaaagctcga tataatcttt
     4501 tactggtaat tccaattcta ccttaaaaaa gtgcaaaaca gccgacagcc cttctgcttg
     4561 atagatttct cctaatgtta gcccctctag atggaactct ggtgaaaaat gcagcaatgt
     4621 tttattttcc tgcaattgaa catatagaaa tgtagatcgc tcaatcagag tatcttttaa
     4681 aacaactaaa aaattcattt ttctccccct tttttcatca tttttaaaat agcatatttt
     4741 gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt cataagtatt
     4801 cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct tgtttgtgtt
     4861 tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta ttttcatcta
     4921 aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata ccaatgactt
     4981 tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg atgccttgac
     5041 caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac acaatatctt
     5101 tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca gaacctcttc
     5161 catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata acatcttcgc
     5221 ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca agatctttca
     5281 tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt catcttttcg
     5341 tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa tcattgcaac
     5401 cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga ttgccacgct
     5461 cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt tatcaaaagc
     5521 cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc cgtatcctgg
     5581 gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca atcctactgc
     5641 tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc tcccctcgct
     5701 caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc ctactaagaa
     5761 ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg caatagcact
     5821 gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga aaaggatcaa
     5881 cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt tgcgcatacg
     5941 aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg ccaaaatgac
     6001 ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca ttggaagatc
     6061 acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac cattccaaaa
     6121 ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc aagggccaag
     6181 ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc aatcggcata
     6241 aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat agccatcaat
     6301 tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa tactccagtg
     6361 ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga tatatcatat
     6421 acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat cccgatagca
     6481 aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt ccctactaga
     6541 tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat tccgatcatc
     6601 atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg tgcaaaaaca
     6661 aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa
     6721 ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt
     6781 cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc
     6841 aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta
     6901 ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc
     6961 agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga
     7021 agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac
     7081 taagggccat aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata
     7141 ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc
     7201 taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa
     7261 gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa
     7321 aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg
     7381 aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat
     7441 ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc
     7501 gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg
     7561 aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt
     7621 attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct
     7681 taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt
     7741 gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct
     7801 actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct
     7861 gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg
     7921 gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc
     7981 aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc
     8041 gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc
     8101 tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc
     8161 caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc
     8221 ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat
     8281 agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg
     8341 gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt
     8401 gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct
     8461 tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata
     8521 ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc
     8581 atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct
     8641 ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa
     8701 agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag
     8761 cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg
     8821 tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc
     8881 tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt
     8941 ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata
     9001 agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt
     9061 gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc
     9121 tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt
     9181 ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc
     9241 tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga
     9301 ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa
     9361 aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt
     9421 ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc
     9481 aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc
     9541 tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc
     9601 tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta
     9661 tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac
     9721 aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata
     9781 atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt
     9841 cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt
     9901 ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca
     9961 gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa
    10021 acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat
    10081 ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga
    10141 aa
//
LOCUS       DACEPW010000093        10121 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEPW010000093 DACEPW010000000
VERSION     DACEPW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.094"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(92..1057)
                     /locus_tag="IW819_001777"
     CDS             complement(92..1057)
                     /locus_tag="IW819_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1244880.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1291..2136
                     /locus_tag="IW819_001778"
     CDS             1291..2136
                     /locus_tag="IW819_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1244881.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2327..3514
                     /locus_tag="IW819_001779"
     CDS             2327..3514
                     /locus_tag="IW819_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1244882.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3520..3876
                     /locus_tag="IW819_001780"
     CDS             3520..3876
                     /locus_tag="IW819_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1244883.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3878..4216
                     /locus_tag="IW819_001781"
     CDS             3878..4216
                     /locus_tag="IW819_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1244884.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            4611..5645
                     /locus_tag="IW819_001782"
     CDS             4611..5645
                     /locus_tag="IW819_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1244885.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5638..6324
                     /locus_tag="IW819_001783"
     CDS             5638..6324
                     /locus_tag="IW819_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1244886.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6348..7196
                     /locus_tag="IW819_001784"
     CDS             6348..7196
                     /locus_tag="IW819_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1244887.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7393..8166
                     /gene="sufC"
                     /locus_tag="IW819_001785"
     CDS             7393..8166
                     /gene="sufC"
                     /locus_tag="IW819_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1244888.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8179..9465
                     /gene="sufD"
                     /locus_tag="IW819_001786"
     CDS             8179..9465
                     /gene="sufD"
                     /locus_tag="IW819_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1244889.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9462..>10121
                     /locus_tag="IW819_001787"
     CDS             9462..>10121
                     /locus_tag="IW819_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1244890.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHMTIDVQALDCDF
                     FA"
BASE COUNT         3348 a         1826 c         2243 g         2704 t
ORIGIN      
        1 aaccagtaca aaaacatgaa cagctgtttt tgtttggccg ttcttattac ttgatgcaga
       61 acagcttttt cacaacctat tgaatcgatt attaagcgat ttttttagaa acaaattttc
      121 catgtttgct atgttcttta attaagaaac gtacgtaatg agcaagaacg tagaagacaa
      181 ctacacttgc aaacagacat tccgcaactt ttaagaaatc caatacgcta ctatagatac
      241 ctaaactgtt gaagtaatga acagtagtaa atatagctgt tgcagagata acaagcggga
      301 atgtaaaggc tgcatagctt ggataaaaac gaagcttgaa caaacgtgga agcatagaca
      361 gcacaacgaa atacaatact tgagataaag cgaacaatgc tacgacaaga taaaaattag
      421 gttcagcaaa gtttttaaga taacctgtta ggcaaagaga accaggtgct gcaacgatcg
      481 taatcagcgg acgtgtcgga tgagccatat ttcttacgaa aaagacacgt ttgatgatga
      541 tcggcaataa gaacgcatag cagatcaatg aaatccaaaa gaaaattttt ccaataagcg
      601 ggaagaagtt cccggcagtt acagtgatga ccccaaatcc aacgtaaatg ataaaccagc
      661 ttggataaat tgcttcaatt gaaagatcag ctttgataac atggtgataa ttgaaataaa
      721 agacaaggat cacttggaag ataacagcgc acagccaaat gaatttgaca attggtgcaa
      781 tactttcata tgaaacaaaa taagtacaaa taaccatgag tgacatcgta aaagttggtg
      841 aaacggaggc gatgattgga tcatgcaacg tttgtttcgt gtgttcgaat aagataaaca
      901 atttaacaaa aatgaatatc ataagaatca tagagatacc gccagttaga ttgcctaaag
      961 caacatggtg atagtcttta aacagatttc caagggaagc taacccaagg atcaacccgc
     1021 agatcgggat cggaataatt ttcaaaaagt tcttcatata taatattctc ctttttattt
     1081 aatagatttt agtaccaaga tgaacgaata cattgtaaaa tgaataggaa tgtatgatta
     1141 cgagcaagca gacagctgat tgtcttcatc taagttacta gttggatcag caactgtttt
     1201 gttgtacatt tctattatag atggtttttt gtatatttaa atcaaatagt ttttatagta
     1261 attatcataa aaagttatgg aagtggtgcc atgtttaagt tattaacgac ttttcgagca
     1321 gtatatgaaa caagaaattt ttccagagca gcagaagtac tgtttttatc tcaaccagca
     1381 gtctcgaatc aaatcaaaca gttggagcgg gaattgaata tcgatctttt tcaacgtaac
     1441 ggaagaaaag agatgcttcc aactaagcaa gccgacttat tatatgaacg gacactggtt
     1501 ttgttagaag aatgggaaga aactcagcaa aaattgctca atgaacaaaa tgaagaagaa
     1561 gtatgtcgga tcgttgcttc ccacaccttt gctgtctatc ttttgcctca gttgatgaaa
     1621 catctgatca aaaggtttcc taaagtgcat tttgagatcg aaatcgcgaa ttctcatgaa
     1681 tcattagaac aagtagcgaa acatgaatcc gattttggtt ttatcgaaaa gccattagag
     1741 acttccggcg tacaaagata ctctttgatg gaagaccagc tagtcatcgc tggggaccca
     1801 aaaagcaaat tgtggcttgt aagagaagca aactctggtg tttttcatta tacgcagcgt
     1861 tattttgaag agatgaatat ccaagataag aagatgatcg tgaaaagtaa cgatgtaatt
     1921 gttgcgttgc tgaaggaagg aatcggtcgc tcgattttgt ctaagcgctc tgttccagaa
     1981 aatgtgccaa tgactccgtt gaatttccat cgatattttt atttcatcca acgaaccaat
     2041 ctggtttctg atcagctgct agagattgcc gaagagatcc ttcgatacaa tgaaagcaca
     2101 gaaacttctc acagcgagga gaatctcagc tggtagataa ttgcatgtat caaaaaaagt
     2161 tctatgatac actaaagata agcagtaggc ttttgtactg catttgctca agcaactgca
     2221 caaacggaaa gttaattttt cccaaatgtc tatggaaaga ctttccatta tctaactgga
     2281 ccgctataat aatcagcagt atggttttta agaaaaggga tgaaacatgg aaaaaaaatt
     2341 aaaagcttcg aacgataatc gtattgatta tggcgtgatt ttaccagtct ttctgttatg
     2401 tttgattggt ctgctctcat tatatgtagc acttagccat gatccgaaca atccagcagt
     2461 cttgaaaggc gtagggatgc aagttgtctg gtatttagtt ggagcggcag caatcgttgt
     2521 catcatgcat ttgaattcca aatggatctg gaaactgact ccttaccttt atggtttggg
     2581 gttagtggta atgggactct tgctcaaatt ctatgatcag aatttagctg cttcaacagg
     2641 gtctaaaaac tggtttcgat tcggtgcgtt tactttccag ccagcagagt tgatgaagat
     2701 tgcgtacatc ttgatgatgg cactgatcgt aactaaacat aatacgcaag taaaagaacg
     2761 gacgatgaaa tcggactttt ggctgattgg gaagctcttg atcgtgacaa taccggtttt
     2821 agcattgatc atggcccaag atgactttgg aacgatgctt gtctttcttg cgatctttgg
     2881 cgggattttc ttgatgtctg gaatctcttg gcgcatcatc gcacctgtag taatcttggc
     2941 agtagtcgtt ggagcaggaa cgatcttctt ggttacgact gaaggcggaa gagacttgct
     3001 ttacaaagtt ggcttcaaat cttatcagtt tgcacgtatt gattcatggc tggacccttt
     3061 ccatgatacg tcaggaatga gttatcagcc ggcccaagga cttttggcaa ttggtaccgg
     3121 tggcttgttt ggtaaaggat tcaacgtaag caatatttat gtgcctgttc gtgagtccga
     3181 tatgattttt accgttatcg gtgaaaactt cggctttatc ggtggtgcat tcgtcatttt
     3241 cttgtatttt attttaatct atcgtatgat tcgggtgtgt ttcgatacga ataatgaatt
     3301 ttacgcctat atcgcaagcg gtttgatcat gatgctgctg ttccatgtct ttgagaatat
     3361 tggagcaaat atcggattac ttcctttaac agggatccca ttgcctttca ttagtcaggg
     3421 aggatcttcg attttaggga acatgatcgg tatcgggttg atattatcga tgagatacca
     3481 aaatgaagca cctgcaagat cttcaaggag gtaagaaaat tgtacacctt ttactggtac
     3541 ccgaaatgtt caacatgcaa aaaagccaaa gcgtggttag accagcatgg cgttagttat
     3601 caaactgttg atatgatcaa agaagtaccg aaggcctcaa cgattgagac ttggctagaa
     3661 acgagtcaag taccaatccg tcgcctgttc aacacaagcg ggatgaaata tcgggaactt
     3721 ggactgaaag atcaagtagg cgagtttacg gcttcaaaag cgagccaagt cttatcgaca
     3781 gatggtatgc tgatcaagcg gccactgatc ttacgagagg accagcttgt tgcaattgga
     3841 ttcaatgaaa aaatttatga aggagaactg atataaaatg gcaggcaagc atttgaagaa
     3901 aaaggacaac ctttggatct tatttaatgg aaaagaatac tgtgttggtt tgacaaatga
     3961 agcgcaagaa gaacttggag acatcacgtt tgccaatctg ccaaaagcag gacaatcgct
     4021 aaaaaaagat gaacctctga tcgaagttga agcggaaaaa gcggtcaatg aatttgccag
     4081 cccgctaacg ggtacagttt cttctgttaa tgataaaatc ggtgaagata tcaatatctt
     4141 gaatgattca gatgaaatga atgcgtggat cttaagtttc aaagatgttg atccaaaaga
     4201 atttgacgaa ttataaaatc agaaaatcac ttgaaaaaag ttgacactga ttcttccagt
     4261 tgctataatc ataagcaatc ctatatgaaa cgagaaagct ttgaaaagaa gagtacatgt
     4321 cacggacgtt tgaagagagc cctgtttgct gaaaatgggt aacgaaaatg agatggaaga
     4381 tggtcttaga gcagagtaag cgagcgtaag caaacttact acgggagtgc ccgttacagc
     4441 gcgacagtat acggaaagaa gctaagtcca agtactggaa gaggctgtta ttgcgaagta
     4501 acggtaaatc aaggtggtaa cacggaagtt caagctttcg tccttttgct taaggcaaga
     4561 gggcgaaggc ttttttgttt gaaaaatcat aagagaaaga tgtgaatggc atgtcattaa
     4621 tccaactaac gaatgtcaaa aagacattca atgggaaaaa cggaaacatc caagccgtca
     4681 atgacgtttc actaaacgtt gaaaaagggg atatttatgg aatcgtcggc tattctggcg
     4741 caggaaaaag tacattagtc cgtctcctga atggacttga gcttcccacg agcggcgaag
     4801 tgatcgtaaa cgatcaagat attacaaaat taaaaaacaa agaattacgt acattccgaa
     4861 agaaaatcgg tatgatcttc cagcatttca acctgttatg gtcaagaact gtacttgaaa
     4921 atatccagct gccgctggaa ttagctggcg taccaaagag caaacgtaaa gaacgggcag
     4981 aagaactgtt gcgtttagtc gggttagaag gacgcggaca agcatatcct tcacaacttt
     5041 ccggcggaca gaaacaacgc gtcggtatcg cacgagcatt ggcaaatgat cctgagatcc
     5101 tgctttgtga tgaggctact agcgcattgg acccgcaaac aacagaagaa gtattagact
     5161 tattgttagc gatcaataag aaattgaatt tgaccatcgt cttgatcaca catgaaatga
     5221 atgtgatccg caaaatctgc aacaaagtcg ctgtgatgga gttggggaaa gtcgtagaag
     5281 aaggagacgt attgactgtc ttcagacaac cgaaaaaaga agtaaccaaa cgctttgtac
     5341 aacaagacat cgaaccagaa gctgattcga cagaactatt agctgaactc atcaaagaaa
     5401 atccaagcgg ccgtttggca actttgacct ttagcgaaag aaatgccaac gagccagtca
     5461 tttcccaagc gattcgacgc tataacatcg atgtcaacgt tgtgtatgga aagatcaaac
     5521 aagtgaaaga aggttccttt ggttcattga ctgtcttgat gactggtgaa accaaagaat
     5581 tagatcaagc agaagtgttc atcaaagaac aaggagtagg aattgaggtg atccatcgtg
     5641 gataaaagct tttcagaaac atatctagat tttaaacaaa tcaacccgga aacgatgaat
     5701 caggctatcc ttgatacact atatatgaca ttaatctcaa tggtcttagt gacgatcatc
     5761 ggattagttt tgggattgat cctttactct attggacgca aaaacaaacc gtatgctcga
     5821 atcatttatg gaatcgtatc gatcatcagt aatattttcc ggtcaacgcc atttatgatc
     5881 ctgatggttt tgatcattcc atttactaaa gcaatcgtag gcactatgct aggtgcgcaa
     5941 gcagccattc cagctcttgt tttatccgca gcaccatttt atgcccgtct agtagagatt
     6001 gcttttcgtg aagtcagccc tggtgtactg gaagcagcag atgcaatggg agcaagttac
     6061 tgggaaatca tctggaaggt cttgatacct gaaagtattc ccgcattgat ttctggatta
     6121 acagttacaa cgatctcgat gatcggattt actgcgatgg ccggcgcgat cggcgcaggt
     6181 ggacttggag gtcttgcttg gcaggaaggc taccagcgtg gtaatctgac agtgacattc
     6241 gttgcgactt taattatttt actaatcgta tttatcgttc aaggaatcgg tgatttctta
     6301 actaagaaaa cagacaaaag ataataggat atagggggat gtaggatatg aaaaagaaaa
     6361 tttttggatt tgcagcagcg cttttgctga ctgtaggatt agcagcatgc ggtaacaaca
     6421 gcgacagcaa agattcagca aataaagcag acgacacgac attgaaagtc ggcgcttcac
     6481 cgacaccgca cgcagagatt ttggaacatg tgaaaccatt attgaaagat gaaggaatcg
     6541 atcttgaaat cgtcaaattc gatgactatg tattaccgaa ccaatctttg gaagaaggcg
     6601 acattgatgc caattacttc cagcatattc cttatttgaa caaagaaatc aaagaaaaag
     6661 gatatgactt tgtcaatgcc ggtgctgtcc acatcgaacc aatgggcttg tactctaaaa
     6721 aagtcaaaga tgtttccgaa ttaaaagatg gtgcaacaat cattacttca aattctgaat
     6781 cagactgggg acgcatcatc acaattctac aagatgctgg cttagtcaaa gtcaaagatg
     6841 gagtagatct ggaaacagca acattcgatg atattgaaga aaaccctaag aacttgaagt
     6901 tcaaccatac gatcgaccca tcacttttag cttctacgta ccaaaatgat gaaggtgacc
     6961 tagttgcaat caacgcaaac ttcgcttacg gcgcagggtt gaatccatta gaagatgccg
     7021 tattgcttga aaaggacaac tctccttacg tgaatattat tgcgactcgt aaaggtgatg
     7081 aagacagtga caaaatcaaa aaactgattg aagtgcttca ccgcaaagaa gtccaagaat
     7141 ggatcacaga caaatggggc ggctctgtta aaccagtaaa ggctgatgca aaataaggta
     7201 tggaacaaaa aagatgagaa ataagtctgt aatggcttat ttctcatctt ttttatacct
     7261 attctaattt agaaatcaaa attgtttaat aataaataaa agatatttga ttttagtaag
     7321 gaaggtcatt gcgcttcatt ctcaattagt ctaaaatgag tgagaacgaa agagaatttg
     7381 ggggtataat gcatgtctgt cttagaaata aaaaatctac atgtgtcgat cgaggaaaaa
     7441 gaaatcctta aaggcgtaaa cctaacaatg aaaaccggag aaatccatgc catcatggga
     7501 cctaacggca caggaaaatc taccttatct gccgctatca tgggcaatcc aaactatgaa
     7561 gtaacagaag gcgaaatttt gtttgacggg aaaaatgttt tagacttgga agtagatgaa
     7621 cgtgcgcgac ttggcttatt tttagctatg caatatccaa gtgagatacc aggtatcacg
     7681 aatgcggaat tcatgcgtgc agcgatcaat gcgaaacgtg atgaaaacga caaaatttct
     7741 gttatggcat ttttgaaaaa gttagatgaa aaaatggctt tactaaatat gccagaagaa
     7801 atggctgaac gttatctgaa cgaaggtttt tctggcggag aaaagaaacg gaatgagatc
     7861 ctgcaattgt tgatgttaga accaacattt gcaattttag acgaaatcga ttcaggtctg
     7921 gatatcgatg ctttgaaagt cgttgctaaa ggagtaaatg aaatgcgcgg agacaatttc
     7981 ggtgcattga tcatcaccca ctatcaacga ttattgaact atatcacacc tgatgtcgtt
     8041 cacatcatga tggaaggccg agtagtcatg acagggaatg ctgacctagc taaacgtctg
     8101 gaagcagaag gctacgctgg tatcagtaaa gaattaggaa tcgactataa agaagaagaa
     8161 gcgtaagggg gagcaagaat gaaagaaaaa aactggctcg attatcttga cgcggtaaat
     8221 gatttttctt taagtaaagg cgaacctgat tggatgagaa ccttccgtca agacgcacta
     8281 gcgaaagcag atgaactgcc attgccgcat atcgatcggg tgaaattcca tcgctggtcg
     8341 ttatttgatg tgaaagaaac acaaaccatc agtgaaacag gcacgattcc ggcatttgat
     8401 gcaatgaaag acaatcctgt gcttgtccaa caaggttcat ggacgatttt tgaacaactg
     8461 cctgtggaat tagcagaaaa aggagtcatt tttaccgact tgttcactgc aatgatcgaa
     8521 tatccagaac tagtccaaga atattatatg aaaaaagcag taaacatgaa cgaagaccaa
     8581 ttgactgcac tccatgtagc ttttatgaac agcggtattt tcctgtatgt gcctaaaaat
     8641 gtcgtgatcg atgaaccact cgagtcctta ttcattcaag atggcgcatc cgatgagcac
     8701 ttcttcaagc atgtgttgat cgtggcagat gaacatagtg aattttctta tctggaacgt
     8761 ttccagacaa ccaaagaaca agtagcaaaa tcatcaggta atatcatcgt ggaagtcatc
     8821 gctaaagctg gttcaaaaat caaatattca gccgtagatc aattaggaga aaatattact
     8881 tcttatatga atcgccgtgg gcacatttta cgagatgctt ctgtcgactg ggcaattggt
     8941 gtcatgaatg acggtcatgt gatcgctgac tttgactcag atttagcagg agaaggtgca
     9001 cacgcagaag tcaaaatcgt ggcaatcagc tcgggcagac aaatccaagg aatcgatacg
     9061 cgagtaacca ataaagcccc tcatacgatc gggcatattt tgcaacacgg agtgatccga
     9121 gaaaaaggaa cattgacctt taatggtatc ggccacatct tgaaaggggc aaaaggtgca
     9181 gatgcccaac aagaaagtcg tgtcttgatg ctttctgata aagcacgtgg tgatgcgaat
     9241 cctattcttt tgatcgatga aaacgaagta actgctggac atgctgccag tgtaggacga
     9301 gttgatccag aagaaatgta ctatctgatg agtcgaggat tgcataaaga agaagcagag
     9361 cgcttagtga ttcgaggatt cctcggttct gtcttaacag caatccctgt agaacaagtt
     9421 cggaaagaat tagtcgaagt aatcgaaggg aagttgaatg gatgatcgat gctaaaaagc
     9481 tacgcaatga ttttcctatc ttatttcaag acgtcaatga tgaaccttta gtttatctgg
     9541 ataatgcagc gacgacccaa aagccaaaac aagtactcca ggcattagcg cactattacg
     9601 aacacgataa cgccaatgtt catcgcggtg tccatacgtt agcagaacgc gcgacccatg
     9661 attatgaaca agcaagagaa aaagtacggg cgttcatcca tgcaaaagaa acagcagaag
     9721 tgctgtttac tcgtggtacg acgacaagct tgaattgggt agccagaagc ttcggcgacc
     9781 gctatatcaa agcaggagac gagatagtgc tctcttacat ggaacatcat tctaatgtta
     9841 tcccgtggca gcagttggca aaacgaacag gtgctgtctt gaaatatatc gaactaacag
     9901 aagaaggctt cctggatatg gaggatgcag cgaaaaagat tacggacaaa accaaaatcg
     9961 tctccattgc tcatgtatcc aatgtcttag gtgtcatcaa tcctgttcaa gaattagctg
    10021 aacttgtcca tgaaaacggc ggggtcatcg tagtagatgg tgctcaatct gccccgcaca
    10081 tgaccattga tgttcaagca ctagactgtg atttctttgc t
//
LOCUS       DACEPW010000094        10095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEPW010000094 DACEPW010000000
VERSION     DACEPW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.095"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW819_001788"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW819_001788"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1244891.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IW819_001789"
     CDS             complement(1172..1867)
                     /locus_tag="IW819_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1244892.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IW819_001790"
     CDS             complement(1880..2596)
                     /locus_tag="IW819_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1244893.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IW819_001791"
     CDS             2926..3534
                     /locus_tag="IW819_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1244894.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IW819_001792"
     CDS             3859..4182
                     /locus_tag="IW819_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1244895.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IW819_001793"
     CDS             4364..5734
                     /locus_tag="IW819_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1244896.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IW819_001794"
     CDS             complement(5864..7165)
                     /locus_tag="IW819_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1244897.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IW819_001795"
     CDS             complement(7519..9510)
                     /locus_tag="IW819_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1244898.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IW819_001796"
     CDS             complement(9654..9920)
                     /locus_tag="IW819_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1244899.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2957 a         2097 c         1834 g         3207 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctgcg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcg
//
LOCUS       DACEPW010000095         9811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEPW010000095 DACEPW010000000
VERSION     DACEPW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.096"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(34..2154)
                     /locus_tag="IW819_001797"
     CDS             complement(34..2154)
                     /locus_tag="IW819_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1244900.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="IW819_001798"
     CDS             2384..3562
                     /locus_tag="IW819_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1244901.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="IW819_001799"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="IW819_001799"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1244902.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="IW819_001800"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="IW819_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1244903.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="IW819_001801"
     CDS             complement(4915..6168)
                     /locus_tag="IW819_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1244904.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="IW819_001802"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="IW819_001802"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1244905.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="IW819_001803"
     CDS             7746..9092
                     /locus_tag="IW819_001803"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1244906.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="IW819_001804"
     CDS             complement(9400..9786)
                     /locus_tag="IW819_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1244907.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DACEPW010000096         9601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEPW010000096 DACEPW010000000
VERSION     DACEPW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.097"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(9..530)
                     /locus_tag="IW819_001805"
     CDS             complement(9..530)
                     /locus_tag="IW819_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1244908.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="IW819_001806"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="IW819_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1244909.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="IW819_001807"
     CDS             4610..5497
                     /locus_tag="IW819_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1244910.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="IW819_001808"
     CDS             5494..6663
                     /locus_tag="IW819_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244911.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="IW819_001809"
     CDS             6687..8042
                     /locus_tag="IW819_001809"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1244912.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..>9601)
                     /gene="glmS"
                     /locus_tag="IW819_001810"
     CDS             complement(8114..>9601)
                     /gene="glmS"
                     /locus_tag="IW819_001810"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1244913.1"
                     /translation="ELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRV
                     IKGSYAFALIDRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDE
                     EMVTVTSEKVIIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIA
                     QKYVTEDNRVSLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLA
                     SEFGYNTPLLSKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREAS
                     FTLLLHAGPEIAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAM
                     ESVIAQKDYLEELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGE
                     LKHGTIALIEEGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLV
                     LPTVHPYLTTLTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         3020 a         1810 c         2135 g         2636 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagcacttg gcggctgtct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 c
//
LOCUS       DACEPW010000097         9571 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEPW010000097 DACEPW010000000
VERSION     DACEPW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.098"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            622..2001
                     /gene="brnQ"
                     /locus_tag="IW819_001811"
     CDS             622..2001
                     /gene="brnQ"
                     /locus_tag="IW819_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1244914.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(2157..2603)
                     /locus_tag="IW819_001812"
     CDS             complement(2157..2603)
                     /locus_tag="IW819_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1244915.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(2630..2806)
                     /locus_tag="IW819_001813"
     CDS             complement(2630..2806)
                     /locus_tag="IW819_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1244916.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(2968..3396)
                     /locus_tag="IW819_001814"
     CDS             complement(2968..3396)
                     /locus_tag="IW819_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1244917.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            3488..4348
                     /locus_tag="IW819_001815"
     CDS             3488..4348
                     /locus_tag="IW819_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1244918.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(4443..4736)
                     /locus_tag="IW819_001816"
     CDS             complement(4443..4736)
                     /locus_tag="IW819_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1244919.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(4809..6536)
                     /locus_tag="IW819_001817"
     CDS             complement(4809..6536)
                     /locus_tag="IW819_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1244920.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(6750..7676)
                     /gene="trxB"
                     /locus_tag="IW819_001818"
     CDS             complement(6750..7676)
                     /gene="trxB"
                     /locus_tag="IW819_001818"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1244921.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(7821..9254)
                     /gene="ascB"
                     /locus_tag="IW819_001819"
     CDS             complement(7821..9254)
                     /gene="ascB"
                     /locus_tag="IW819_001819"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1244922.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(9254..>9571)
                     /locus_tag="IW819_001820"
     CDS             complement(9254..>9571)
                     /locus_tag="IW819_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1244923.1"
                     /translation="ARNVAFGSLPAAERGWLQVMKNMELLTIEAAVTGDYGTALQAFT
                     INPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQDELARELSSNTHVM
                     EEV"
BASE COUNT         2646 a         2052 c         1706 g         3167 t
ORIGIN      
        1 taaaagagtc gtttaaccat acgtaccaag aaacgttaaa aaatagccgt ttccattcgc
       61 taaaggtctc gttttgataa aaagaatctg agaatgtaag ttatatgaaa gtggagaaca
      121 aaatattccg tttcattcag caggtattct ctaggtattt ttcttgtttc aaaaaaagaa
      181 aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca ggtgtattat tacgtcaaca
      241 aataaacata cagcaaagcg tcgaggaaaa cagtaggcta ttcaacgatt tattcccagt
      301 gagtcatgta agtgagaaat gacaataaat cgaatagacg aacatcattt ccgagtcaat
      361 gtcgaaaacg tagatcattg tggctgcctc cattatcagg ctagcggata actttatcaa
      421 aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa ggtggtaccg ttctagaatc
      481 tagagccctt ttcccaagtg gaaaagggct ttttttatat tttaaggtgg tggttttcat
      541 ggaatctgat attaatggca ttactaattg ctgcaacgtt tatatcgtca atcaaaatga
      601 taattattgg aggaaaaatt tatggaagaa acaaaaactt attcatggaa acaactattg
      661 atggtcagtt cacttatctt cgggatgttc ttcgggtcag gtaatctaat ctttcctgtc
      721 cacttaggtc aaatggctgg cacaaactgg gtcagtgctg gcgtaggatt tgccatctca
      781 ggcgctcttt tcccattact agctatctta gctgtagtag tgacaaaaag tgatggtctg
      841 tatgacttag ctaaacctgt cggaaaatgg tatgctgcct tgttcttaat ccttgttcat
      901 ttgacgatcg gaccattttt cggtacacca cggacagctg ctactgcttt tgaaatggct
      961 gctaaaccat ttttgcctga aaaatataat tcattaggta tgtttttatt ctctgctctt
     1021 ttctttttac ttgcttattt ccttactgtc catcccacac ggttggtcaa atacgtcgga
     1081 aagtatctaa actccttgtt tcttgcatta ttagcaatca tttttgtcgt ttctttattc
     1141 cacccaatgg gaaatctaaa tcaatctgct gcagatactt accaaacaaa cgctttaaca
     1201 aatggtgtcc tagaaggtta taacacggtt gatgcagtag ctttgcttgc cttaagtgtc
     1261 acttttgtcc atgcagtccg tgaccttggt ttcaaagata aaaaagtcac agaattaaca
     1321 gcaaaagctg gtagcctgag cgttgcgcta gaagtcatga tctattttgg acttgttctt
     1381 ctcggtgcga tgagtttgaa taagatgtct ttatcagaaa atggcggaac tgcactttct
     1441 cagattacta atttttattt aggtaacttc ggcgctagct tcttaggagt gatggtcaca
     1501 ttaggtgtct tcactaccgc tatgggactc gttgtttctt ttgcacaaga tttccataaa
     1561 ctgttcccta aagtcagtta tatgacatgg ctgcggttaa cgactttcgt ctcttttgtc
     1621 gttgcaaatg ctggtttgga caatatcatc caatggtctt taccagtatt gatgcttttg
     1681 tacccattgt cgcttgcgtt gatcttgctt tccttaacag caaaattttt ccaaaagaca
     1741 ccatttgtct atcaagtaac gatgttgttt gctgctgttc ccgccgtatt ggatatgctg
     1801 gcaaattcgc ctgctttagt gagtcaacaa agagttgtag caagcatgct cgaattttat
     1861 catcatcacg ttccttttgc tgcattagga cttggctgga tggtcccaac gttacttggt
     1921 tacgctggca gcttactctt ctattacgcc tatcgcctat cagggtacaa acaagaagca
     1981 aatgaactac cagaagaata accagatcaa acacaattta acataagaaa tattaaacag
     2041 gaaaaaacca gctagatgaa agaatcctat tctttcgtct agctattttt tagatttctt
     2101 ttttacgagc acaaaaaaac tgcggacaat gattgtccgc agtttaaatg ataaaattaa
     2161 gattgtaata gttctttcac aatggcattt acttggtttc catctgcttt tcctttgact
     2221 ttaggcatga tcgcacccat gactttgcca aattctttca ttgatgaagc accagtttgt
     2281 gtgattgctt cttgaacaag ttgtcgaatt tcttcgtcgg aaagttgttc cggcatgtat
     2341 ttttcgacta tcacaatttc actttttact ttttcagcta aatcgtcacg tcctgcttct
     2401 tcaaactcat gcaaagaatc acgtcgctgt ttcatttcgc gagacaacac cgttaattct
     2461 tcttcttctg tcaagtcgtg accttcttta atctgctcgt tttgtataga tgacttgatc
     2521 attcggatga cttgtaaaga ttccttatct ttcgctttca tcgctgtttt catgtcatcg
     2581 tttaatctac ttagtagtga cacactacca actccaattc atcacagaac tagaattttt
     2641 tgcgttttct agctgcttca gattttttct tgcgtcttac acttggtttt tcgtagaatt
     2701 cacgtttacg agattcttgt aaagtacccg cttttgacac ggaacgtttg aagcgacgaa
     2761 gagcatcgtc aagtgattcg tttttgcgaa cgactgtttt tgacatgtta attccctccc
     2821 tccgagctta aaaagtaacc gtaattgtta ttgaaatata tctcaaaaaa caagactgtc
     2881 ctgtaaacat tatatctaat gctgttttga ccgtcaagga aatcttgctt ttttttaaaa
     2941 ttatcaaatg ttattttatt taaatcctta ttgacaattt tcacatcttc cgaaaatctc
     3001 gaatctatgc ccttcgatcg tacatccacc gagctgttct tcgaaataat tcattggaca
     3061 catatggatt tctttcgttg ttccgcacac cgtacagata aagtgatggt gatggccgat
     3121 ttccccttga cagcaatgaa aacgaaactt catctctcca tttaaatcgg tttcttccag
     3181 taaatcgatt tctgcaaaat catggagatt tcgataaatc gtatcgtaac ttactccttt
     3241 gaattcctga ttcatatagt caaaaacttc tttagcggat acgtagcgat tgcgcttcac
     3301 taaataagtt aacaatgctt ctcttttttt agtatattta aaaccgttat ttttcaatgt
     3361 ttctagtgat tgttcgatca atgtctgttt tttcatgact tattcctcca aatcaaaatg
     3421 attacgaagt acacattcta gagtatggta taataaacat aaaaaaaatg caagtgaggt
     3481 tttatccatg tcttccgatt ctacagaaat cgttactttt tttgtcatct ccgactctgc
     3541 tggagaaact gcgacaaaat tagcgcaagc cactatggcc caatatccta gcgttgagtt
     3601 caatttattc cgcagaacat ttgtgacaga tgagaaaaca ttgatgcagg cactgcaaga
     3661 tgcactagaa gaaaaagctc ttgtcttaca cacattgatc aatcaagaat tgatcgagat
     3721 gacgcgttct ttttgtgaag aacatcaatt atttagtttt gatgtgatga ctccgccgat
     3781 atcagaaatc gagcgcttaa ctggtatcaa gcccattcgg caacctggcg cccttcattt
     3841 attgaatgag aactatttca aacggattaa agcaatggaa tttgctgtaa aatacgatga
     3901 cgggaaagat cctcgtgggt ttctagaagc agatgtcgtt ttgctaggcg tatcccgaac
     3961 ttccaaaacg cctttgagct tgtttttagc taacaaaaat ttaaaagttg ccaatcttcc
     4021 tttgatccca caagcacata ttcccaaaca gctttgggag attgatccta aaaaaatcgt
     4081 gggattgacg aataacccag atattttaaa caatatccgt aaagaacgga tgcgctctta
     4141 cggattaaat cctgatactg cttattctga tatcgaaaaa atacgagctg aactcgattt
     4201 tgcaaatgat ctttatgaaa aactgggctg cgtagtaatc aatgtcgctt ctttatctat
     4261 tgaagaaaca gcttcgctta ttttgaatgc tttagaccta gaagatcata gttattatgg
     4321 aactgaaacc accgaagaaa cagactaaca gtttttcttg tctttttctt aaaaaaaact
     4381 ggactgtgtc agcgaagaag attcgttgac acagtccagt ttgtctctat ttgcacctat
     4441 cgttattgat gttccacatg tttgagtgtc tggttcaaaa tatccagtac gtgagaatca
     4501 tcaagactat acaaaatcgc ttttccctca cgatgggact tgacgacatg gttttctctt
     4561 aataaacgca gttgatgcga tacagcagat tgttccattt ccactgcttg ggtgattgct
     4621 gtcacatttt gttccccgtc tttcagatac agcagaattt tgagacgagt ggggtcactc
     4681 aacattttga atagttggct gactttctct acttttgttt tttgcgtgtc tggcaatcca
     4741 ttcacctcca acttgaaaaa agactgactt ctaaagttta ccactttttc catcagtctc
     4801 aagttttttt attcttctgt catcttttgg ataatcgctt cataagcggc tactttttca
     4861 gctgcgtctt ctgaagagtt tccttttaca ccgatgtaaa atttgatttt cggctcagtt
     4921 cctgaaggac ggatagcgat ccagccattt tcttccagcg tatacttcaa tacatcagat
     4981 ggcggtgtcg ttagtttttc tacgtttcct tcacggtctg ttctcgtcaa ctctttgaag
     5041 tcttctgttt ggatgacttt tgctccggca aattctgttg gtgcttcttc acggaatttt
     5101 tccatcaaag cagagatttt cgcagaacct tcttgtccgc tcattgtcac agaaatcgtt
     5161 ttttctgcgt agtagccata ggtttcaaaa atcgtttgca agccgtcata caacgttttg
     5221 ccttgttttt tatagtaagc tgctacttct gctaataaga gcaatgcttg gatcgcgtct
     5281 ttgtcgcgaa cgaaaggttt aactaaataa ccgtaacttt cttcgaatcc gaacataaaa
     5341 gtatgggaat gatcttcttc aaattgttga attttttcag caatgaattt gaatcctgtc
     5401 aatacattga acatggtagc cccatagtgt tccgcaattg ctgtaggtaa ttcgctagaa
     5461 acgatcgatt tcaataccgc tgcgttttca ggtaaagtgc ctcgcgattg gtgtgcttcc
     5521 aaaatatatt gagcaaacag tgctccgatt tggtttccgg tcaatacttc ataggagcca
     5581 tttggcaaac gaacggctgc acctaaacgg tctgcatcag gatctgttgc aatcagcaaa
     5641 tcagcgcctt ctttctcgcc aagtcgtatc gcatactcaa aagcagagtg ttcttctgga
     5701 tttggtgatt tgactgtagt gaaatctggg tctgcgacag cttgttctgg aactagtacg
     5761 aattgttcga atccagcttg tttcaacgca cgttcgccca acatttttcc tgttccgtga
     5821 agtggtgtgt acacaagttt caaatctttc cccattgttt ccaccaattc atgatcaatc
     5881 gttactgctt tgatttcttc tagataagcg gcatccactt cttctccgat gatcgtgatc
     5941 aatccgctat gttcagcttc ttcgtcagat aatacttcaa cttttaacgg attgctgact
     6001 tcacggacat atttcgtcaa tgcatctgcg tctgcaggag gcatttgtcc cccatcttcg
     6061 ccgtagactt tatatccgtt gtaagctgct ggattgtggg atgcagttac catgattccc
     6121 gcaaacgtct tcaaatgacg gactgcaaaa gaaagctctg gtgttggacg tagactttcg
     6181 aatacataag atgggatatt gtgatgtgct aatgttttgg ctgcttccat ggcaaattct
     6241 ggtgattgat ggcgtgagtc ataagcgata gcgacacctc ggcgttttgt ttcttcgcct
     6301 tgtgtatcca tgaaacgtgc aagtccttct gttgcttgtc ttaccgtata gatgttcatt
     6361 cgattgatcc caggccctaa gattccccgc atgcctgcag taccgaattc caacggtgca
     6421 taaaaagcat cttcaagttt ttcaggatct tcacccagat catttaattg tttttttaag
     6481 ttttcttcta aattttcttc atgtacccat tgttcataca cttgttccca agacatgaaa
     6541 ataacacctc tttctgaaat atggttctta ataagtatag cattcactta ttagatataa
     6601 aagtatgagg gccaaaaaac gcttaaatca agaaaggatt cttttatcaa gcacggttgc
     6661 aagtattcgc ataaatcgga ttcagtagat ttcacgcgaa aaaaaagagt aaaagaacaa
     6721 ttgaaaccgt tcttccactc tttttaaact tatgcagttg tttcttctaa agattccaag
     6781 tatttataca cttgttgtcc agcgatccca ccatcaccaa cagcagtagt gatttgtcgc
     6841 aaatcttttt cacgaacatc tccgatggca aaaacgcctg gtatattcgt cttcatttcg
     6901 ttgtctgttg gaatccagcc agcttcgttc gtcaaccctg cttctctaaa tggtgcagtt
     6961 aatggatcca gtccaacata gatgaatact ccgtctgctg ccaaatcaaa ctcattgccg
     7021 gttttgacat tacgtgcttt cacacctgtc actaccattt cattcccaac gatttcgtca
     7081 acgactgtat cccacataaa ggaaattttt tcattcgcaa atgcacggtc ttggatgatc
     7141 ttttgtgcac gtaattcatc tctacggtgg acgatgacta cttcagaagc aaattgtgtc
     7201 aaatagatgg cttcttctac tgctgagtct cctcctccga tcactactag acgtttgtta
     7261 cggaaaaacg caccatcaca aactgcacag taagaaacgc cacgtccagc aaattcttcc
     7321 tcgccaggta cgcctaattt acggtggata cagcctgttg cgataatgac tgtttttcct
     7381 tcgtaagttt tatcttcaca aataacttct ttgtggctgc cgtgatcctt gatatcttgc
     7441 acgattccat aagcattttc tgtcccaaat cttgaaacac cttcatacat cttataagct
     7501 agatcaggac ccatgattga atcgaaacca ggatagtttt cgatttctgc cgtattgttc
     7561 atttggccgc caggggcgcc ttgttcgatc atcaatacgg ataagtttga acgtgaagca
     7621 tataaagcag cagtcatccc agcaggacct gcgccaataa cgattacatc atacatatgt
     7681 agattctcct ttacttaatc aagtaagcat actttacagc caatgcgaaa aaaagtctat
     7741 tgttctgctt gcgtttctcg aatagcttta ttatactcga aaaatccgct gaaaaacaat
     7801 agacttttat aaatcattga tcaatcattc gaaagatttt ctccatttga atcaattact
     7861 tttttatacc agttgaatga tttcttcttc gaacgggcta atgtgccatt tccctcgttg
     7921 tcacgatcga cataaatgaa tccgtacctt ttcttcatct cacctgtacc agctgatact
     7981 aaatcaatac atccccaagt cgtatagcct aatagatcga ctccatctaa ttctactgca
     8041 tctttcattg cttggatatg tgctgccaga tagtcgattc gataatcatc ttccacataa
     8101 ccattctcat cgggcgtatc gactgcacct aagccgtttt ctacaataaa tagtggtttt
     8161 tgataacgat cataaatatc attcatcgtt attctcaagc ctaatggatc aatttgccaa
     8221 ccccattcac ttgcttccag atatggattt ttgacagaag caaagatatt tcctgcagtc
     8281 tgttccaaga gttcgttgtc agtagtcgaa actcgagaag aataataaga aaaggagata
     8341 aaatcaacag tatgttcttt cagcaatgcg gcatccccag gttccatttc gatctgaatg
     8401 ccatttcgtt ccatttcttt caatgcataa gcgggatatt ctcctcgtga ctggacatca
     8461 atgaagaaat aattttctcg atctgatttt ctagaagccc aaacatcttc aggattgcaa
     8521 gtgtatggat aatatgctcc tgctgccagc atacagccta ctttgttctc cggatctact
     8581 tcatgagcaa tcttcgttgc gatggcactt gcaaccaact catgatgtgc cgcttgatat
     8641 ttgatttgtt cttcattttc tccttcttca aaatacagcc ctgcgcccat gaatggtgca
     8701 tgaaggatca tattgatctc attgaatgtc aaccaatatt tgacaagtcc tttatagcga
     8761 ttgaagatga ctcggcacag attttcataa aaaccaacga gttctctgga acgccacgca
     8821 ccatattctt tgatcaaatg catcggacaa tcaaaatgtg taatcgtcac aagcggctcg
     8881 atcccgtact tttgacattc tttaaagaca tcctcataaa atttcaaccc agattcattt
     8941 ggctcttttt catcgcccat tggaaaaata cgactccaag caatggacat ccgataagtc
     9001 ttgaacccca tttctgcaaa taaagcaata tcttccttat aatgatgata catatcgatc
     9061 gcaatttttg ctggataaaa atgattttca tcaaattcaa acattttctt ttttccagta
     9121 atgacaggaa aacggtcttc tccgattggc acaacgtcta cgttcgctaa cccacgtccg
     9181 ccttcatcat aaccgccctc gcattgattg gctgctgtag ctccgcccca taaaaaatct
     9241 tttctgaatc ccatcatact tcctccatta catgtgtatt gcttgataat tctcttgcca
     9301 gttcatcttg gacttcgatt ccttctgctt ttaaccggct gattgtttcc ttgaattgcg
     9361 gtaaatactg ctgatgtgca ataaacaact catccattat ccgttttgct gtctttcctg
     9421 atcgtataag cggattgatc gtaaacgctt gcaaagctgt tccataatca ccagtcacgg
     9481 ctgcttcgat cgtcaacaat tccatattct tcattacttg caaccagcct ctttcagcag
     9541 caggaaggga accaaacgct acatttcgtg c
//
LOCUS       DACEPW010000098         9509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEPW010000098 DACEPW010000000
VERSION     DACEPW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.099"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(277..639)
                     /locus_tag="IW819_001821"
     CDS             complement(277..639)
                     /locus_tag="IW819_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244924.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(713..1093)
                     /locus_tag="IW819_001822"
     CDS             complement(713..1093)
                     /locus_tag="IW819_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1244925.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1175..2350
                     /locus_tag="IW819_001823"
     CDS             1175..2350
                     /locus_tag="IW819_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1244926.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2427..3011)
                     /locus_tag="IW819_001824"
     CDS             complement(2427..3011)
                     /locus_tag="IW819_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1244927.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3212..4210
                     /locus_tag="IW819_001825"
     CDS             3212..4210
                     /locus_tag="IW819_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1244928.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4322..4831
                     /locus_tag="IW819_001826"
     CDS             4322..4831
                     /locus_tag="IW819_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1244929.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4861..5520)
                     /locus_tag="IW819_001827"
     CDS             complement(4861..5520)
                     /locus_tag="IW819_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1244930.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5522..6286)
                     /locus_tag="IW819_001828"
     CDS             complement(5522..6286)
                     /locus_tag="IW819_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1244931.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6294..6971)
                     /locus_tag="IW819_001829"
     CDS             complement(6294..6971)
                     /locus_tag="IW819_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1244932.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7008..8393)
                     /locus_tag="IW819_001830"
     CDS             complement(7008..8393)
                     /locus_tag="IW819_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1244933.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8603..8687)
                     /gene="ffs"
                     /locus_tag="IW819_001831"
     ncRNA           complement(8603..8687)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW819_001831"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="IW819_001832"
     CDS             complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="IW819_001832"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1244934.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9332..>9509
                     /locus_tag="IW819_001833"
     CDS             9332..>9509
                     /locus_tag="IW819_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1244935.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVIND"
BASE COUNT         2850 a         1909 c         1621 g         3129 t
ORIGIN      
        1 tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt tatcatgtat
       61 ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt cgatcaatta
      121 ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca ctaaaaaact
      181 atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa gaagtttgaa
      241 aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca ctccctctac
      301 ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat atccttcgcc
      361 taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa cattttcctc
      421 tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga tatgaactat
      481 ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa gttccgctaa
      541 cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac catcctccaa
      601 atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct cgatcaactt
      661 gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact ttttatttct
      721 tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt aactgctccg
      781 caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt ctgcgatatg
      841 gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt ttggaatatt
      901 catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat gaatggatat
      961 ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc tcgttatcaa
     1021 gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt atttgtccaa
     1081 ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa atagctcaaa
     1141 aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata aacagatttc
     1201 aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc aaatggatga
     1261 cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg tagctaaagc
     1321 gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg ataaactata
     1381 tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga aagaaaatat
     1441 cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt ttacaaaacc
     1501 aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta ttatagaaac
     1561 aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt tcatcatgga
     1621 cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga ttttatgcaa
     1681 tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt taagcgaatt
     1741 gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt taactttatt
     1801 cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc tactagttgc
     1861 ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa tggtatttat
     1921 caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc aacagcacga
     1981 aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg gagaatggtt
     2041 aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt tttttgaaca
     2101 gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt ggttagattt
     2161 taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga aaggaaaagt
     2221 cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc gcttgaatat
     2281 tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg ctttagagac
     2341 aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt tcgtcctttt
     2401 tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc aatcactaca
     2461 tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc tactgtgtcc
     2521 atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg agttccgcca
     2581 cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg tccaagcata
     2641 ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt gtttggacct
     2701 gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa ttcatcttca
     2761 aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc gccgccttgg
     2821 atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc ttttttggaa
     2881 agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg aacagtaatg
     2941 tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa atctagttgt
     3001 gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa taacataata
     3061 cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac tagatacatt
     3121 ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc tcaattaaat
     3181 tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta caatcatcgg
     3241 tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag ctaaaacaaa
     3301 aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc ccgaaaaata
     3361 tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg atcaattaga
     3421 aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc tctcgcttac
     3481 tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt ccaaagcagt
     3541 cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag ataatgctga
     3601 atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt atgctggaaa
     3661 aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga tgttagagcc
     3721 tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc acgaacatag
     3781 tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca tcgatggatt
     3841 atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa gtgacgagac
     3901 gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta atttagaaca
     3961 tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat cagatatgtc
     4021 tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag gaaaagtgaa
     4081 actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt tacactatat
     4141 taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata aaaatcttcg
     4201 tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc agtttcttgc
     4261 atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg ggagcaataa
     4321 tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga aggaacgtgg
     4381 cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact atatttctga
     4441 tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc gtgaagtaca
     4501 taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa aattgatgag
     4561 tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg agatcctagc
     4621 cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt atatcgacaa
     4681 ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg tccatacctt
     4741 cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag cacacaaaga
     4801 accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga aaacgtctat
     4861 ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga aaagaaaaac
     4921 agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga aaaattcttc
     4981 tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat cgttgttgaa
     5041 aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga aaaaaccgaa
     5101 aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa gccgatcatt
     5161 cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa acacagcata
     5221 atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag cagaaactgg
     5281 gaaatctggc aagctgagga tggttttgaa gggattattg agataattca ttaggtgatc
     5341 aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt gcaaatgtcc
     5401 gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca gactgatcag
     5461 tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg aatttttcat
     5521 attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata ggcaacccag
     5581 gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata ttattgcgta
     5641 tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct tctttttgca
     5701 gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg atgtagatcg
     5761 gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca ggaagaaggc
     5821 cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca cctttttgaa
     5881 tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag cctaccacta
     5941 catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag tcaatcaatc
     6001 ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata aaatcgattg
     6061 ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac tcatctgcta
     6121 gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac aagaaaggta
     6181 attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt tgtgtcccac
     6241 gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat ccactactct
     6301 tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg ttgttcttgt
     6361 ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat aaccggtttt
     6421 ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt atggtttttt
     6481 ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc gatgacaaaa
     6541 tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc gattcgctga
     6601 tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag ttgttcataa
     6661 ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg ttctccaagc
     6721 ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct ttcacctatc
     6781 aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc tgctttaggc
     6841 tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt ttcctgttca
     6901 gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg ttcattttgt
     6961 tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta tttgattggg
     7021 tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa caaaaattca
     7081 ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg atctacggta
     7141 gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac ttgatggtta
     7201 atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc gcgaaatccc
     7261 attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc taaaactaag
     7321 cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg cggcaacgca
     7381 gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa cagaccactg
     7441 tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc tacttggata
     7501 aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc tacttctttt
     7561 tcttttaata attcatgccc acgttctaga taactattta tttcttgttc atcttctgta
     7621 aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat ttttttctct
     7681 tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc ggctgctatt
     7741 tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga tcctaaaata
     7801 acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc gagatgactc
     7861 atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg caaaatatca
     7921 taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg agcagtaaat
     7981 gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg tttcgtccat
     8041 gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc aaactcagca
     8101 tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt gtttcctatc
     8161 gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc attcgttgct
     8221 tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat cgttgtcgtt
     8281 ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat ccttggccaa
     8341 ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc catgtaccct
     8401 ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg aaacaagctc
     8461 tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc gataaatcag
     8521 tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa aaaaacagac
     8581 ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc ttagtgctgc
     8641 ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt cggcaaagaa
     8701 tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt ttcgctggcg
     8761 gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa tgccaccttc
     8821 tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg taccggctgt
     8881 tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca aaatcatcgc
     8941 gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt ccaagcgcca
     9001 gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg tagtagcatc
     9061 ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca atacaaccac
     9121 tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca atgcaaggcg
     9181 catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc cttccctgcc
     9241 tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa cttttttttc
     9301 tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac ttgctcttta
     9361 cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt cttttgcaat
     9421 tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa ccagcctttt
     9481 gaaagcgtta ttccctgtaa tcaacgatc
//
LOCUS       DACEPW010000099         9328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEPW010000099 DACEPW010000000
VERSION     DACEPW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.100"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            21..1183
                     /locus_tag="IW819_001834"
     CDS             join(21..297,297..1183)
                     /locus_tag="IW819_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1244936.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            1463..2443
                     /locus_tag="IW819_001835"
     CDS             1463..2443
                     /locus_tag="IW819_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1244937.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            2565..3983
                     /locus_tag="IW819_001836"
     CDS             2565..3983
                     /locus_tag="IW819_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ1244938.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4054..5523
                     /locus_tag="IW819_001837"
     CDS             4054..5523
                     /locus_tag="IW819_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1244939.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            5533..6000
                     /locus_tag="IW819_001838"
     CDS             5533..6000
                     /locus_tag="IW819_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1244940.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6036..6908
                     /locus_tag="IW819_001839"
     CDS             6036..6908
                     /locus_tag="IW819_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1244941.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            6990..>9328
                     /locus_tag="IW819_001840"
     CDS             6990..>9328
                     /locus_tag="IW819_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1244942.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHL"
BASE COUNT         3205 a         1362 c         1883 g         2878 t
ORIGIN      
        1 aacacactgg aggaattttt atgtcaagac gtcaacgtag aacctattca aaagaattca
       61 aacaacaagt cgtcgatctt tatctcgcag gaaagccccg agctgaaatc attcgagatt
      121 atgagctgac tacctcggcc tttgataagt gggtcaagca agcgcaaact actggctcgt
      181 ttcaggaaaa agataacttg acccctgaac aagtagaact aattgcatta cgaaagaaaa
      241 ataaacagct tgaaatggag aatgacattt taaagcaagc ggcgctgata ttgggacgaa
      301 aagacaagta attgatacca ataagcacaa atactcaata tcagcgatgt gtaaagtgtt
      361 aaacatctct tgccaaacct attattatca agccaaacca gttgaagatg aatcagaact
      421 ggaagaactc gttcaagaag aatttattcg caatcgaaaa gcttatggta ccaggaagct
      481 gaagcagtgc ttggccaatc taggtcttca agtgagtcaa cgcagaatcg gccgtatgat
      541 gaaacagcgt ggcttgcgtt caacatacac attggctcat ttcaaattcc attcttctac
      601 ttgtaacgaa gccaagaccg ccaatattct agacggaacc ttcctacagg aaaagtcatt
      661 agaagcgatt gtaaccgacc ttacctatgt tcgtgttggg aagaaatggc agtatatctg
      721 cctgattctt gatttgttta atcgagaaat catcggctat tcatgcgggg agaagaaaga
      781 tgcgacgtta gtcaaggaag cttttgcaaa gattccgtac tcgttgaata atgtacaact
      841 ttttcacacg gatcgtggaa aagaattcga taaccagacg attgacgaaa ttttatgggc
      901 ctttaatatc acgcgttcct taagcaaaaa aggctgcccc tatgataacg ccgttgcaga
      961 atccacctat aaatctgtca aagtagaatt cgttcatcaa taccgctttg agacattggc
     1021 taatttgcga ttagagctat ttgattacgt ccattggtgg aataccatcc ggctgcacgg
     1081 cacgttaggc tatgagacac cgatcagttt tcgacagcag agattggcgc agcgaatcct
     1141 tgataatgag ctcggatgtg ataccggtgg cgaggcacct taattatttg aatgagcttc
     1201 tgccttagag aatcagattc gaggaggctc gttgtcaagg gcagtcggaa caccagcagc
     1261 aagcttacgc aataccttat aatttttgtc aaaaaaagtg ttgccattcc acatatatct
     1321 ttaaacagct attgccagat aaaaataaat gatattatat atataaatac ttatccgaaa
     1381 aggggaatga gagagtcaag gttcttggaa tgtcgaatag tcgagtaggt agattttcta
     1441 tctacaacct tgttgtttaa aaatgaaaaa aattggaatt agagaagttg cgaaagaggc
     1501 aaatgtttct cctacgactg tgtcgagggt attgaatgat cgtggatatt tgagtgagga
     1561 aacaaaaaga aaagtttttg atgccatgaa aaaattagaa tactatccta atgaactggc
     1621 gagagcactt tttaaaaacg aaacatatac cgtaggtttg atttttccta caattactaa
     1681 tccatttcac gctgagttaa ttcaagatat cgagttcgag ctttccagca ggggatataa
     1741 agtgttgctg tgcaatagct taaacaatcc aacaaaagaa aaagagtact tgacgatgtt
     1801 gagaaagaat caggttgatg gaattatagt aggaacgcac aataaagata ttgcggatta
     1861 tgatattcca gggctaccta ttgtagcgat tgatagaagt cttggtgaaa atacagttac
     1921 agtatcttgt gataattaca ggggtgggga attagctact caactacttg taaatcatgg
     1981 ctgtcagaat atactgtgta ttcggggaga tagtaagata aagctacccg cgaatagtag
     2041 gactttagca tataaagatg ttttgaataa attcatgctg gaaccgttag tcctagaagt
     2101 accatttgta aaagaaatca atgaaaaaaa tgaaataatt aagacatatc tggaaactca
     2161 tccaaatatt gacggggttt ttgcaggaga tgatttattg gcttctttgg cgattaacaa
     2221 cttaggaagt tcgggcaaaa gagttccaaa agaggttaaa gtgattggat ttgatggagc
     2281 tcaacaaaca ttgatttata atccagaatt atcaactatt caacagccta tagatcaaat
     2341 atctaaagta gcagtaaaaa aattattgaa tatgattaag ggcgaaaagg agacggatga
     2401 actttcactt ccagtaaagc tcataaaaaa aaatacagca taagttaaat cggcgaaaaa
     2461 ttccttcgtc gatttaaaaa tatttttatg aaacgggttg acatatgaaa cgggttgaca
     2521 tatattagtt gtgaagataa aaaccttttc actggaggaa gaaaatggag aaattattaa
     2581 ttaaaagcaa taatcatcaa ctttactacc aacctaagga tgtttgggtc ggagatgtta
     2641 tgccttacgg gaaagaagga aaattcttta tttatcatca acgtgatact agaaatccag
     2701 ttccttttgg agaaccgttt ggctgggctt tagcaactac gaaagatttt gttcattttg
     2761 aagactttgg ggaatctctt ttaagagggg atgacaatga gagagaccag tttatctacg
     2821 ctggttctgt atttgaggca aatggaaagg ttcacgcatt ttatacaggg tataatcgtg
     2881 aattcttagc gcagggaaag acatctcaag tgttgcttca tgctgagagt gatgattttg
     2941 ttcattggga aaaatcagaa acagcattgg agttagaacc acaagaaggg tatgatattc
     3001 gaaactggag agatccattt gtcgtttggg atgatgaaaa agaagaatat ttgctaattt
     3061 taggggcgcg aaagggtcag gataaacatc tacaaaccgg acgtttagtg gaatttacat
     3121 caaaagattt aactaattgg gaattcaaag gtgatttttg ggctccggga ttgtatacca
     3181 tgtttgaaat gccagaactt ttcaagattg gtgactggtg gtatcttgtc tattcagaat
     3241 actcagatgg aaataaaata ctctatcgaa tgagtcaaag tttggaaggt ccttggatta
     3301 agcctaaaga tgaagctttt gatggacggg cttattatgc aggtagaaca gcttatgatg
     3361 gaaaacgtcg agtcctattt ggatgggtac caacaaaaga tcaagaaaat gataaaaaca
     3421 attatttatg gggaggtact ttggttcctc atgaagtcta tcaaaaagag gatttttcac
     3481 ttggggtaaa acccatagac gaagtgtgga atgcattcat aaatttacat gatattgccg
     3541 atgtagagat agaaacagca gacacaagag aagaagtaac gcttgtcaaa aatagtggaa
     3601 ctttgtttaa tttagaaaca acaatttcat ttgatgagaa tcttagtgac ttttcactga
     3661 gattatataa aaaccttgag acagatgaat cttacgaata tcgcttcaat ttagatgagg
     3721 ggcgtcttac atttgataaa aacccttgtt acccttggtt tcaatatatg gataaaggac
     3781 ttattagacc tatatcttta aagcctaacc aatcttataa actaaaagtg attgttgatg
     3841 acaatataat gacactttat attgatggaa tcgctcttaa cgctcgtgta catggttgtt
     3901 ttggtcaagc aattactatt acagcaacaa atggatcaat gactgcaata aataccaagt
     3961 tttcaaatga aataaagaaa taataaacgt caatcatgtg gcaggtctga aaatcaaaca
     4021 tgacctgtca catttaaaag ggaggataaa tgaatggact acaaaaaaat tgccgaagat
     4081 gtattggaaa acgttggagg aaaagagaat atctcaagcg ctacacactg cattactcgt
     4141 ttacgtttaa ttcttaaaga caaaagcaaa gcgaatatgg aaatgcttga ggaaatagaa
     4201 ggtgcaaaag gtgttctttt caatagtgga cagttacaag taattttcgg aacaggtata
     4261 attgaaaagg tttacaaaga gttcgttgaa ttgacaggag ctaaggaagt atccgtttct
     4321 gaaatgaaag acgaaggagt caataattca ggaaatagac ttcaacgagc ttttaaagtt
     4381 ttctcggata tctttattcc gatcattcca gcttttattg gggcagcaat ggttttagga
     4441 attcgttcgt tgattaccac tcctggaatg tttggaatgg atggctcatt cgctgatcac
     4501 aatgtattcc taaatgattt atccaaattt ctgggtatta ttgcaaccac atttgattat
     4561 ttaccagttt tagttatgta ttctgcaaca aaacgttttg gcggaaaccc tattttagga
     4621 ttacttgtag gatttgttat gattcatcca ggactagcca atcggaatga tttcgtcatg
     4681 ggaactataa agcctgatta ttggcattta tttggattaa gcattccagc agttgcgttt
     4741 caagggggtg tttttccagc aattttgact tcttggtttt tagcaaaagt tgagaaaatt
     4801 acaaataaat attctcctca agtacttgct tacattttag tcccaactgt gacaattgtt
     4861 cttgccaact ttgcactatt tttagtgttt ggtccattgg ggaattttat cggtaatgtt
     4921 ttaggtggaa tcattgattt cctatatatg aagatgggtg cctttggtgc ttttgttttt
     4981 gcaggcgcgt tacaaccatt agttgtaaca ggaacacaac atgctattca aggtattgag
     5041 gcaaacttag tcgcaacaac tggattcaat tatatccaac ctatttggtc agtttcaatt
     5101 attgcccaag gtggcggttg tattggtatg tatttactct ttaagaaaaa atctaaagat
     5161 agagatattg cgatttcaag ctttattcca acactggtag gtattactga accggctatc
     5221 tttgctgtta atttgaaata ttcaatcgtt ccttttgttt gctcgtttat tggtgctggt
     5281 tttggtggag catttatgaa aatttttgat gtaaaagcca ttggacaagg attgactgta
     5341 ttgccaggtc tgactattgc taatccagtt cttccttata tatttggtaa tctcatagca
     5401 ttcattttac caatcatttt tattctcgct tatgataaat ataaagtgtt tatccctgaa
     5461 gctaaacacc gtaaatttgc agaaaaaact gactcaaaaa aattgccagt tcaagtaaag
     5521 tgagatgaga gcatgttaaa tttttttaga aaaaatgaaa taagcgttgt agcacctgca
     5581 accggtgttt ttgaaaaact ggagaaatta agtgatccag tcttttcaaa aggaatgatg
     5641 ggacaaggat ttgcaattga tgcaaaaaac gaaacgattg tatctccaat cgatggtttt
     5701 gtttcaagta tttttcctac aaaacatgcc ttaggaataa aaacaaaatc aggaattgag
     5761 atacttgttc atgtaggaat agatacagtt gagctaaatg gtgagggatt tgatattaaa
     5821 gttcaagaag gacaaaaagt taaggctggg gatattctta tgaccattaa ttttgtagtt
     5881 tttgaaaaga atcacaagac taaagatgtc attgttgttt ttccagacta ccaagacgaa
     5941 attttcattc cgaaacttga agaagtaaaa caaggacaag aattgtttgt tcttggctaa
     6001 tgataagtaa ttaataatag aatcgaggaa aatgtatggt ttttggggct attgaagctg
     6061 gtggaacaaa atttgtttgt gccattggag ataaagattt gaacatattt gaaaaagtca
     6121 gctttcccac gactacaccc aaagaaacaa tgaatttagt tttagatttt tttaatcatc
     6181 ataaagatga tttagaaggt atcggagtgg gatcgttcgg tcctattgat attcattgtg
     6241 aatctaaaac gtatggcttc attacatcaa cgccaaaact tgcttggcag gattttgatt
     6301 ttattggaag tttaaaaaag caacttaata ttccaactgc ttggacgact gatgtaaatg
     6361 cctcttgcta tggtgaatat gtttctggaa atggcagagg cctttcaagt gttgtttact
     6421 ataccgtagg aacgggcata ggaggtggcg ctatccaaaa cggaaacttc attgaaggtt
     6481 ttagtcatcc agaaatggga catatgcttg taaaacgaca caataaagac aacttttctg
     6541 ggacatgtcc attccacgga gactgtttag agggcttagc tgctggtccg gcaatcgaaa
     6601 gaagatcagg gaaaaaggga caagaagtga ctgaaaatga cgttacttgg gaaatagaag
     6661 cttattacat tgctcagtgt gcatataata caacattgat gttatcacct gatgtcatta
     6721 tatttggtgg cggagtaatg aaacaaaaac acttaaaaaa gaaagtacaa gataagttta
     6781 ttgagttgat caacggatat gtcgctattc ccgatgttga caattatatt atgacaccaa
     6841 aattgttaga taattctggt attgtaggtt gtttagcttt agcaagagac gttaaagaaa
     6901 aatcttaatt tggaaaaaag atcgtctcga ctatttgttt ggttgatgcg atcttttttt
     6961 tcaattagta attgaaggga cgcctaaaag tgaaaaaaaa aatgaaagat gaagtattag
     7021 agtatatcaa aaataattta aagtattacg atttttctgc tcaagatatt gcaatgaaat
     7081 tttgtattaa aagaaatgtc gcttcccact atttgaatca gttattttca gatggaaaat
     7141 tactaaagaa tgattctgtt agaccagtaa tgttcaagta taatcaacaa aagccaaaag
     7201 actgcttttc taagtttatt ggagcagaca tatctttgaa atccactata gataaatgca
     7261 aagctactgt aatgtatccc cctaatggcc tcccattaat aataaaagga aacagtggcg
     7321 ttggaaaaag ttttttagca agtttgatct atcagtatgc tcttgataga aaagtaattc
     7381 ataatgacgc aaaatttgtt gttgtgaatt gtgcagatta tgcaaataat ccagagctat
     7441 tgtcagcagt tttatttgga tataaaaaag gagcctttac tggggctgaa aaagatactg
     7501 cagggatgct atcttctgct aatggaggat accttttttt agatgaggtt cacaatctaa
     7561 gtgcagaaaa ccaagagaaa ttgtttcttc ttatggattc tcaaaagtat cgtaaacttg
     7621 gtgaaagtgt gcaatgggag tatgcaaatg ttcgccttat cttagccaca acagaagata
     7681 aaaatacctc tttgttagca acttttagaa gacgcatccc agctgaaatc actctgccag
     7741 attacaataa tagaagtaca tctgaaaaaa ttcaattatt atttgaattt cttaaagatg
     7801 aagcaattac aattgattca aaaatatttt gttcgattga actttttact gatcttttat
     7861 cgaaaacatt tgaaggaaat gttggagaat tgaggaatga aatcaagctt ctttgtgcag
     7921 aaggttattt aggaaataag agaaagtctt caatttattt aggggacaaa ctagacagcg
     7981 gtttttggat agatccagaa attagtatag atttaaaaaa aatgtttctt aaatcacttt
     8041 ctgaaattga tttgattgaa ctttttcaaa atcacatttc aattgaagaa agcatgagtc
     8101 aattaagaga tagaatactt aaagagtgtg caggttcttc tgagtataat tatttagaaa
     8161 gtgacgaatt tatagcatta agaaactatg ttgttaataa aatatcacca attattgatt
     8221 cgacaggtct atgtttatta gatgaattct tggcggatat cagtctattt ataatgttta
     8281 ttgatgtaat tgaagaaaca tcaagaaatt ttttgctgtc taagttcaga aaatttcgta
     8341 tgaaaaatga taaacaaaaa ttattggcaa atgagatttg gagtagttta gaagtcgaag
     8401 aaagtaaaca agaacttttg ttgaaatgga tactttttct tgtaaagaag ttctatgtga
     8461 aaataccaga aacccattgt ctgatagtta tgcacggtaa aataacagct tctgcgatcg
     8521 caagggaaac aaataagttg ctgaatacat atgtttatga ggctttcgat atgccaattg
     8581 aaggtgagac tagtgactta attaacctaa tcaacaattt ttgtaaaagt atagatacga
     8641 cgaatggact cattctgcta gttgatatgg gatcattgga acaaatgtat gaaaaaattg
     8701 aatccaatgt aataggtgat ttggtaattt taaacaatgt ttctacagct ttagcgattg
     8761 aatgtgggat tcaagtttgc caaaaaaagc caattagtca tttttaccag atggattttg
     8821 attcgtttca agttaaagtt cagtattata aaggattatc acaaaagaaa aatgtaatag
     8881 tatcttgtct ctctggtgag gggatttctg aaaaagttaa ggatatttta aaacggtatc
     8941 tcgataatca agtggagatt ttgatttttg attttaacgc attaaagaaa atagcaaagg
     9001 aaaaagatac tatgattttt aagaatacaa tttgtgttct ttcaaccact gaattttaca
     9061 ttcaaggaat cgattgttta aatttagaaa atctaattaa tgggaatcaa accttagaaa
     9121 aattaaataa gtatatggac tcggatagct gtgagttctg cttaaatgaa ttagttaaac
     9181 tctttactat tgaaggcgct agtactaagc ttcgcttttt agattccaaa aaagtattca
     9241 atgagataga aaaagtacta tatttgtatg aaaaatatta cgatgtcaaa atcccaagtt
     9301 ttctgagaat taatctattt ctacatct
//
LOCUS       DACEPW010000100         9155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEPW010000100 DACEPW010000000
VERSION     DACEPW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.101"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..881
                     /locus_tag="IW819_001841"
     CDS             24..881
                     /locus_tag="IW819_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1244943.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(952..1809)
                     /locus_tag="IW819_001842"
     CDS             complement(952..1809)
                     /locus_tag="IW819_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1244944.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(1794..4496)
                     /locus_tag="IW819_001843"
     CDS             complement(1794..4496)
                     /locus_tag="IW819_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1244945.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(4509..5798)
                     /locus_tag="IW819_001844"
     CDS             complement(4509..5798)
                     /locus_tag="IW819_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1244946.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            5948..6994
                     /locus_tag="IW819_001845"
     CDS             5948..6994
                     /locus_tag="IW819_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1244947.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            6996..9149
                     /locus_tag="IW819_001846"
     CDS             6996..9149
                     /locus_tag="IW819_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1244948.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLLELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2618 a         1904 c         1669 g         2964 t
ORIGIN      
        1 attgcactga aaggagtaca cttgtgaaaa tcacgatcac tttacctatg aaccatcctg
       61 aaacaacaat tcgcgacttg cttgaaaagg aatggttagt tcctagaaag gttcgacact
      121 ttttgcgtat acgtaaaaac gtttggatca atcaacaacc tgctttgttc catcatgaag
      181 taaagggcaa tgaccagatc acattgttat tcgaagaaac agattataat tatcaagaag
      241 ttcaattagg agatgcgaag cacatccgtg ttttatatga agatgagcat ttgattatag
      301 tggataagcc tgcaggaatg aagactcatc caaatgaacc gacagaaaac agtaccttac
      361 tcaatcattt agctgcttat ctcgacacta aacagcaacg accttacgtt gtccaccgct
      421 tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa tccatttgtc ttgccgatac
      481 ttggaagaat gttggaacaa aaaatgatct atcggagata tcaagccatc gtatggggag
      541 aaattgccaa agatatgacc atcaccaata aaattggacg agatcgccat gatcggagaa
      601 aaagggtcgt agatgataaa aatggtcaga gcgccttgac acatgtcact gtcaacaaag
      661 tactttctga acaaacacaa gtctattgtg ttttggaaac cggacgtaca caccagatca
      721 gagttcattt agctcataag ggacatccga tcgttggaga ccctctctac caaaatagaa
      781 cagctgaacg actgatgctc catgcattag aactgcacat gatccatcca tttactcaag
      841 aaaaaatagt cggtaaagct ttacctggat tatgggaata gaaataaaaa agaacagagt
      901 cttatcagca gataatgata agactctgtt ctttttgtgt aatttgtctg cttacttttg
      961 atgttcgtgt tctgctaaca gcaaactacc gataatccca ctttcattcg gcagtcccca
     1021 acgaacgata tattcactta cttgaggtgt ttccatataa ccacccatca attcaacaaa
     1081 ttgctgacgg attttttgaa ggagatgatc ctgattcatt accccaccgc ctaaaataat
     1141 cttttcaggt gctaacgtca acgtatagtt gatcagtgct tgagcaatat aataagcctg
     1201 aatagcccaa acaggatgat cttgcggcag atcttgccct ttgatccctg tgcgtgcctc
     1261 aatagacggt cctgctgcta atccttctaa acaatcttta tgataaggac aagtaccttc
     1321 ataatggtca tctggatggc gtttcaccca aatatgtccc atttcaggat gtgcgatccc
     1381 tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt cctgttccca cggtaaggta
     1441 aatacaacta ttttttcctt gtgctgctcc ttgcttcaat tccccatatg cagcagcatt
     1501 gacatccgtt gtccaagcag caggaatatt gtagcgttcc ttgattgtgc caagaaaatc
     1561 gaattgtgcc cacccttttt tgggagttgc caaaacgtat ccgtaattat cttttgtttt
     1621 gtcgatacca atcgggccga atgagccaat cccgattccc ttcaattcat aacgatcaaa
     1681 aaattcaaat acctgatcca atgtttcttc tggtgttgtg gtagggatac ttaatttttc
     1741 taaaatttct ccaccatctg aaaccgcaca cacaaatttt gttccgcctg cttctattcc
     1801 gccatacatt cgttttcctc ctcgaatccg attgtccgaa tctcataagg ttggattatt
     1861 gggacaaccg cttgcttcgt tggttcttca agcagattca atatgacagt ttttccattg
     1921 tctttagtta tttccaattt ttctaaatga ctagacatat tgaaacctct tactactact
     1981 ttatcactaa acttgctgcg tttcaaagct gtaattgcaa acgtttcact ttttatcgtt
     2041 aaatattgtt gttttgaaat caatgttcct tcatgatgtt tgatttgttg agtggaaaaa
     2101 ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt tttcttctgg tccatgtagt
     2161 tcaatgctgt aattgaaccg atgctcacct agacactgtg cttctggtgt tggaaaatat
     2221 ccccaatcgc ctagttctcc cacactgcga agtagcgtca ctgcgatttg tccgtcttct
     2281 gttacttcat attcattcaa accaaagttt ccgactgtta caccgtactc ttcatcatga
     2341 atattgacaa atgcttgctg atgttgcgga ttcgtggggt tttcccaact tttagacaca
     2401 tggtttgggc gcttcaccac ttcaaaaata ctatctgctt cgtgatgttc cacatgtaac
     2461 tttgtaggaa acaagacacg taagcgatga tctttcatct gattgtcgag agtcgtttca
     2521 aagtcgattt ttttactatc ttttctgatt gtcatgattg tttttatttg aaggattcgt
     2581 ttttctttgg atcgttctgc ttttcgataa cgaaattcca caaccatctg ctgttctttt
     2641 tctaaacgct cgtctgcaga aacagggatt tccatttcct gtagaatact tatttttgct
     2701 atttccggtg tatctactaa tacttttact tcagagtttt ctttgttact ggataggatt
     2761 ggctgatcac agaaaggttg tttaaagata tactcatttc ctatatcgcc gatatcttca
     2821 aaaatatgca gattattcaa ctttcgtttg tttttgcgat cttccattgt aatggtacca
     2881 tttttttcaa tcgtcaaatg tagagggccg ttttcgatga ttcgtccact ctgatgaatc
     2941 attgtttcct tttcttctgc tttcgcttcc ccttctacaa gcgcgaatgt ttcccaagaa
     3001 aatgctggca tgttttctaa tggtaaagtc actttgatca ttcttgccat atatggcaca
     3061 cggaaacggt ctttcggcaa atcataacca aattggacag tttcttcaga taaaatagct
     3121 ggtatagtgg ccccactttt tgtttctaca tgatacttac gtttaggcaa attttctaac
     3181 tcttgatata gttgttcagg tattccttca gcgaatcttt ttctttctaa ttcgatggtt
     3241 atttctgctt caccagtttt atccattcct gcagtattta caatgacaaa tggaaagctg
     3301 tcttttggga atccggtcgt atcgattgct gcggtcagct gttctaagga atcttgtgca
     3361 agatactttc caacttcatc tgctttttca aaacgaggta tcatttcccg atggacagaa
     3421 tcgactgaac aaccgcaaat actgtcgtga ggatgatttt gcattaatgt cttccaagca
     3481 taatctaatt gatcatgggg atagtttcca ctaacttcat aagccatcgt cgcaagtggt
     3541 tctgtaatat tttctagctg tcgctggacc ttggtattcc attgttttaa gtaaacccga
     3601 gcagatgctg tattggctaa agtataccag ccatccgttt cttgacttgt caattctcct
     3661 tctactgagc ctaagtcttc tggcacatct ttttgaactg cttccagata gtcagtaaaa
     3721 ttagaatgaa taaactcgta atctggaaac agttcatttg cgagatggat tgcttttgat
     3781 atatcttttt gtaccggttg atgatcaaca ccattcatca tcaataagtg gttcgtagag
     3841 gcatattgtt ctgcatccgc taatttttgc ttccaaaagg caagagctgc ttctttttct
     3901 actggaatct cattcccgtt gctataccaa ttggcaaata gcagaccaaa gattgctgtt
     3961 tgatcagggc ctttccacca catctctgag tattgggaag aatagttttc tgcttccaaa
     4021 acttgattat caaagccaat tggttttaca ccacggccaa acgctgctgc tgaaatacca
     4081 gcttgtttca ttaattgagg cgtctgtccc atatttccaa atgtatcggg aaaatagcct
     4141 aacatgacag gggttcccca tttacggctt tcttccatgc caatcagcat atttctcacg
     4201 ttcgattccg agctgataag aaaatcatct tgcaagatat aaaatggtcc aattctcaat
     4261 tttccttcat tgattgcttg ttggattgcc tgtctttttt ctggccttac ttgaagatag
     4321 tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt tgaaggacgg atcaacttca
     4381 aatagctcta gtagatcgtc tatcaattca atcagtcgca tgtgatgctg ttcgtaagcc
     4441 atgtaccatt cacgatccca gtggctatga gaaatgatat atactttctt tttcatttac
     4501 gatacctcct acttttctac tctaatatca aagtaatcca tgactaattc gcaaaacatc
     4561 atatttgccc aagaaaacca ttctctcgta taattttcag gattgttcac atcgaatcct
     4621 tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta aattcaatat ccgctctttt
     4681 tcagatttgt ctgacgttgt cattccttcc atagctaatg caatcggcca aacgtatttt
     4741 tctggcgtat gggaggatcc aattcctttt gcgtactttc cttcatagaa atatggattt
     4801 tctttactca acaatgttct tcttgtttgc agataatctt cctcttgttc agaacaatat
     4861 cccaagtaag gtgcggcgat cagattgggc acattactgt catccatgac tgtggcgttt
     4921 cccattccat ctacttcata tgcataaatc gtttgccctt tttgattaac cgttcgtccg
     4981 tgattctgaa tcccctcttg gatctctttc ttcaatttct gtgtttgctg tacaatttct
     5041 ttatcatcga gtatcgtttg aaaaatttct tccacataac ctaacacgac caccgcaaac
     5101 atattggatg gtaccagata tccataacgg caagcatcat cgctaggacg gaaaccagac
     5161 catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt cattgatcag cgtatcttct
     5221 tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt catgatcttg ctctgttttg
     5281 aacacgtcca agattttttt gataccttca ataaaagatt gattgaattg atcagttttt
     5341 cctgtatttt tgtaaagaag ataggccaac tgtattgggt agcataaaga atcaatttca
     5401 tattttcttt cccagatcca atcgttcatt tttgtatgat ctgtttggtg gccagcaccg
     5461 ttagcttctt cgttgaaggc attcgcatat ggatcgatat tgatataaaa aaattgcttt
     5521 ttgactaacc cgctgatcat gtcagctaat tcctcatctt cttttgcaat cactaaataa
     5581 ggacgaactt gtgcagtaga gtctcttaac cacatcgctg gaatgtctcc cgtcaataaa
     5641 aaggtggttc cgtcattttg ctttttgact gttgtcaaca gagtattggc aaatgctgct
     5701 gtaaaattct ttgcccagtc cgcatggata tccccacatt tttgtgtgat atcttccata
     5761 aatttctgca ccgattttgg tatatcttta taaaccacta ataaaacctt ctttctattt
     5821 gatatatctt taaagattat acttcataca gccaattatg tctacgcttt caaaaaatat
     5881 ctttttattt actttttcat ccattatggt attatttgaa tatgatatat cattaagagg
     5941 tgtttatatg actcaaccat tgtaccagac aatatttaat gatttgcaaa cggctatttt
     6001 aactggtgaa ttacctgtag acagccaact accaactgaa aaagaacttt cacgtaccta
     6061 taaagtaagc agaatcactt ccaaacgtgc attgactgaa ttagagcaat taggacttat
     6121 ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct tctccggaac agattcttgc
     6181 ttctcctaca aaaacacgac ggattctgtt tcttcttcca tatgtcgctg atttgtctgt
     6241 tggtgatttt agcaagggat tgaaccctgt gatgcaagat aaacagattg atgtcatgat
     6301 gacgacgctt gactttctac aaaataaaac agcaaaagag ctaatcaatg aatttgacgg
     6361 attgatttat tatgctcttg atacagaaca acatcttgac ttactttttg agctttcatt
     6421 gaaagaattc cctgtcgtca tattagataa aaaaatctat gagttacctt ttcctaccgt
     6481 tctttccgat aatttccaag gcggtgcgct tgctgcctct tatttgatcg aacagggaca
     6541 ttcaaaaatc gcttatttgt ttggtacgca gactcatcca caatccgttc gacagcgtta
     6601 ccttggttac cttgaagcat tggacaaagc tggtttgact ttccacactt cactggatga
     6661 caaaagcgcg acattagatg aattgctttc ttatatcact aagtatgaag taactgcttt
     6721 tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat ctcttgagac agcatggtta
     6781 tcatatccct gaagattttt caattatcgg atttgatgat atccaagcag cagctcttgt
     6841 tgacccgcca ctgacaacca tcgcacaaga ttttgaacag ttaggtaagc ttgcgggaga
     6901 tgctttggtc acttggttag agacaaaaga tcgaccatcc gatagtaaat ttccagtttc
     6961 attcatcaaa agacaatcaa caaaggagaa ataaaatgaa tatcggtcaa atagatacta
     7021 gacatgcaac agcgaatcag ccaaatttct ctaatgggaa ctgccagcct tatactggcg
     7081 ttccttttgg gatgaattat tttgcaccgc aaactacgga tcaaaacggc agctggtggt
     7141 ttcatcctga tgaccgcgtt tttcaaggct accgtcttac ccatcaacca agtccttgga
     7201 tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg aaaactacag gagaacaccc
     7261 ttttccatgc tcaaagttct taccgcccag aagaaagtat attttgcccg actcatctgt
     7321 cgatctgtca attacgttat ggcatctgtt ctacattgat acctagcatg tatggcggaa
     7381 tcttatctat tgattacagc aaaaatgaca gcggtttact tttatccttt ccgggacgcc
     7441 atcagctctt tgttatagat ccttacacag tggaaggaaa aatcacacat tttgctggtt
     7501 cagaagatcc tgatttttct ttttatttta tattgagatt tgaatatcct ttaacaattg
     7561 atgagttagc tgtttccaac aaaaatagcg actcgatccc gttgtatttt gaacagacaa
     7621 aaaaacaaac tatccgattt gggacatctt ttatcagctc ggaacaagca gcttataacc
     7681 tggaactaga acaaaaccaa agcaaagaga cttaccttgc tcacagtcaa gaacaatggc
     7741 agtcctattt tgatcgtata cagctcacac accatgatca acaacaattg cggacgtttt
     7801 atcataattt atatcgggtg tttttatttc cacaaacatt ttatgaaatt gatcctagtg
     7861 ggcaaaaaat ccactatgat acgactagtc gatctataaa acctggtgtt ctctatacaa
     7921 ataatgggtt ttgggatacg tataaaacgg tttatcctct ttattctttg attgctgtag
     7981 aaaaatacga agaaatgctg gaaggttttt tgaacagcta tagagaaagt ggctatttgc
     8041 caaaatggct gtctcctgat gaacgtggac ttatgcctgg gacattgatc gatgcggtca
     8101 ttgcagatgc tgctgtaaaa gatatccgac gagacttgat gcctgaattt ctaacggcaa
     8161 tggaaaaagg tgctacggtt caaagcaaac atccaaatta tggacgccaa ggaacagaag
     8221 attacttgac atatggctat gttcctaatc actaccacga atcagtcaat cacacattag
     8281 actatgctta tagcgatttt tgtattagcc aagttgccaa ggtactagaa gaagaggaaa
     8341 tgacgaagaa atatcagcag caatcattga actatcaaaa tatctttgac tatgagtcag
     8401 gatttatgcg ttcaaaagat aaagatggca agttcagaga accattttca gctaatcgtt
     8461 ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag ctttgctgtt tatcacgatt
     8521 tttcaggact gatcggaaaa tatggtggca gtgagagata taaagaaaaa ctccttgagc
     8581 tgtgcaatca gcccccaacg tttgacccag gaggatatgg ttttgagatc cacgaaatga
     8641 gtgagatggc cgcgatagaa tttggacaat tagctatttc aaaccagcca agttttcact
     8701 atccttactt gttcagttat ataggacaac ctgaaatggc acagccactg cttaaacagt
     8761 tgatgactca gaccttcgac gattctccaa tgggttaccc tggagatgag gacaatggaa
     8821 gtatggccgc ttggtatatc ttcaatagtc tgggatttta tcctgtcacg cctggaaccg
     8881 gtgaatatgt tatcggtatg ccattaattg acaaggctgt tttgcttttg tcaaatggaa
     8941 aggaattgac gattgaagca acgcctaatc ggccgcagca acaatttatc cataaaatca
     9001 aacggatgga cagagaatat acgaaattat attttaccca tcaagatttg ctgaaaggcg
     9061 gcacgatctc ttaccaatta gggatcatcc ctcaccctaa gaaatatcaa gcaaaagatt
     9121 tgcctttttc cttatcaaac tataattaac aagag
//
LOCUS       DACEPW010000101         9111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEPW010000101 DACEPW010000000
VERSION     DACEPW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.102"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..349
                     /locus_tag="IW819_001847"
     CDS             <1..349
                     /locus_tag="IW819_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1244949.1"
                     /translation="AKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDL
                     SKNEYRLLFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQG
                     YIETKVGQGYIVP"
     gene            367..1392
                     /locus_tag="IW819_001848"
     CDS             367..1392
                     /locus_tag="IW819_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1244950.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            1481..2347
                     /locus_tag="IW819_001849"
     CDS             1481..2347
                     /locus_tag="IW819_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1244951.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            2384..3760
                     /locus_tag="IW819_001850"
     CDS             2384..3760
                     /locus_tag="IW819_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1244952.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(3861..5057)
                     /locus_tag="IW819_001851"
     CDS             complement(3861..5057)
                     /locus_tag="IW819_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1244953.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(5181..5843)
                     /locus_tag="IW819_001852"
     CDS             complement(5181..5843)
                     /locus_tag="IW819_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1244954.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKDEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            6071..6634
                     /locus_tag="IW819_001853"
     CDS             6071..6634
                     /locus_tag="IW819_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1244955.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIINVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRSKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6647..6931
                     /locus_tag="IW819_001854"
     CDS             6647..6931
                     /locus_tag="IW819_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244956.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            6950..7642
                     /locus_tag="IW819_001855"
     CDS             6950..7642
                     /locus_tag="IW819_001855"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1244957.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            7654..8199
                     /locus_tag="IW819_001856"
     CDS             7654..8199
                     /locus_tag="IW819_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1244958.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDICLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            8471..>9111
                     /locus_tag="IW819_001857"
     CDS             8471..>9111
                     /locus_tag="IW819_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244959.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGVSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGPDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKDHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGS"
BASE COUNT         2754 a         1583 c         1983 g         2791 t
ORIGIN      
        1 tgcaaaaatc aatgcattac ttcgccgttc ttacaactac acagaattat ctagtgaaat
       61 gatgagccac aatggcatta cgttgaatgt tgacaacgga agtatggaaa tcaacggaga
      121 gatcattgat ttgagcaaaa atgaatatcg tctattattt attttgatga agcacaatgg
      181 aaagatctta agtcgggaaa aattgctgcg agcactgtgg gatgacgaac gatttgtaga
      241 tgacaataca ttgacagtca acataaatcg gttacgccga aaaatcgaac aggcaggtat
      301 ccaaggatat atcgaaacaa aagtaggaca gggatatatc gttccctgaa aggatgagcg
      361 gttaagatga atttttttaa atatttgaaa gatcagtgga cattgatctt gggatggctg
      421 ttttttattg cattgacgat ctttgtgatg tggctggcgc caaacatcaa agtggatttg
      481 agcacaattg cttatttagc tttgattgag ggcgtctttc tgatttttta tctgctgggt
      541 ggttacttga gcaagagaag atggtgggga aaattagccg acatacaaaa aacttcccct
      601 ttgcaaaact atttgagcgg tggacgaaca gaagaagaaa aacttgtgga agaatatatc
      661 aatcagctga tccgtgaaca ccaagaagta atgcaatcag caatcagtaa tcaacaagac
      721 caaaaagatt atattgactc atgggtccat gaaatcaaag tgccactttc cgcttctaaa
      781 ttgctagttc gttcgatcga gttcgatatt gacgatcaaa agtatacatt actagaaaat
      841 gaattatcaa aaatcgatga atacgtagaa caagttttgt attatgcaag actcgacagt
      901 ttctcgcggg actacctcat ccaagaatat ggactaaaag gaatcattca gcctgtgata
      961 cgcagtcagg caaattattt tatccaaaaa aatattcgct acgagattgt tggtgaggat
     1021 catactgttt tgaccgatgc taaatggatc gcttttatct tcaaccaatt gcttagtaat
     1081 gccatcaaat atacgccgga tcacggaaac atcattgtga gcatagaaaa ggaagcacaa
     1141 ggagtcagcc tttcagttaa agattcaggg atcggtatcc cagcggaaga cttgaaacgg
     1201 atctttgata agggatttac tgggagaaat ggtcgactta gcaaaacaca ttcaacagga
     1261 ttaggacttt atttagcaaa aaatctggcc gaaaaattag gtatccattt gacggctgaa
     1321 tcaactgaag gaaaagggac aacaatgact ttatttttcc caatcttaaa tttttataat
     1381 gaaaaacgtt agaaaatcgt tagaaacgaa aaaaagtgct tgtgttgtta acgttttcgc
     1441 tgataagttt cttatcatgt gaaataaaag gagattggtt atggaaattt taatagcatt
     1501 agtccctatg ttggcatggg gtagtattgg tttagtaagt ggtaaaattg gtggcgatgc
     1561 caatcaacaa acactaggaa tgacgatcgg tgcatttctg ttttctttag ttgtcttttt
     1621 cgttgttagt cctgtaatca ccccttggat tttcttgatc ggatttttat ccggcttagc
     1681 ttggagtgtt ggacaaaatg gacagttcca tggaatgaag tacatgggtg tttctgtagg
     1741 gttaccattg tctactggtt ttcaattaat tttgaatacg attgcgggag ccgttttctt
     1801 ccatgaatgg acacaaacaa aagattatgt atacggtatt attgctttag ctcttcttgt
     1861 aagcggtgcg tatttgactg cccgacaaga tgacgaagga aaaatagata cagataataa
     1921 aatgcttgat tttggtaagg gttttagagc attgatcttc tctacgattg gttatggtgt
     1981 atataccatc atcgtcaact gggcgaattt ggatgcgatg tcaattatct tacctcaaag
     2041 tatcggaatg atcttaggcg caagcttctt tgctttccgt aaagttaaag tcgatcagtt
     2101 tgtgtggaaa aacatgatct gtggattatt atggggctta ggaaacatct gcatgcttct
     2161 aacagtcaaa tcacttggtt tagctgttgg cttctcactt tcacaaatgg ggatcattat
     2221 ctctacttta ggtgggattt tcatccttgg cgaaagaaaa acgaaaaaag agctcattta
     2281 tgttattgtt ggttgccttt tagtcatttt aggtggtatc cttttagggt atatgaaaac
     2341 agtttaatcg ctgttttcca acctaaaatg gggaggaact tcaatggatt tgtttcgtaa
     2401 gaaagagatc aatctcaatc atacgagtga gatgaaaaaa gaactaaaaa cgtctgattt
     2461 gatcatgctt ggaattggtg cgatcatcgg tacgggaata tttgtggtga caggggttgc
     2521 ggccaatcaa aatgctggtc cagcactgtc actttcattt gtcttagctg caatcgttgt
     2581 cattttgtct ggactaagtt tcgcagaatt tgcttcgcga gtgccagtga ttggtgggcc
     2641 atatgcctat ctgtatgttg tatttggtga atttgctgcc tggctgacag ggtggttgtt
     2701 gatcggggaa tttttactag cggtttcctc tgttgcttcc ggatggtctg gctatatgca
     2761 aggttttttg aaaagtttag gggccgaact tccacaagca ttaactggcg ggtacaatcc
     2821 ggaaaatggt acatacattg acttgattgc ggtgctcgta gtgatattcg tcacttacat
     2881 cgtgtcgctt gaagccaaga aagcattacg tttgaataat gccatggtat atgtcaaatt
     2941 tggtatcatc gcgttgttta tcattgtagg aatatttttc gttaaacctg ataactggca
     3001 gccattcatg ccttttggtt tttccggtgt gctagatggt gcagctcttg tgttttttgc
     3061 tttcttggga tttgatgccg ttgcaatggc agccgaggaa gtcaaaaacc cgcaaaaaga
     3121 tgtgccaaga ggaatcattg gttcgatact gattgctact gtcctttaca tcatcgtgac
     3181 gctgatcttg acaggaatcg ttccgtatac tgaactagga gtcaatgacc cagtggcttt
     3241 tgcaatgagg tatgtaggac atggaacagt gggcgcggtg atcgctgtag gggcaatttt
     3301 aacgctactg acggtaacga tctcgatgat gtattcactt gctcgtttac tgtttgctgt
     3361 cagcaaggat ggcttacttc ctaagtttat gacagaaatc gatcaaaagc atcgtacacc
     3421 gaaaaaagcg acttatgccg ctggtgtggc agctgtattc tttgccggat ttttcccatt
     3481 gaatgtatta gcggaactaa cgaatatcat ggcgctcgcc tatttgatgc ttgttagtct
     3541 aggcttactg aagttgagaa aaatgtttgg caaaccaaaa gctggagaat tcaaagtgcc
     3601 attcgttccg ctattgccaa tcgtctccat cttgacttgt attgtattga tgcttcgtct
     3661 gcaaactgtg acatggatcg tgttcggtat cgtaatggtt attggactga tcatttattt
     3721 tggctatggc tatggacaca gcaagatgaa tgagaaatag actccaatgg agaagttaga
     3781 agaaagataa gaaaaacgcc caacaacagt ttattacaac tgttgttggg cgtttgtttt
     3841 agtaacaagc gaaaggtgat ctattgggtt aaatccaaca gtttttgttt cagatcttct
     3901 tccatgtgtc caagctctac ttctgcatga cgacgttttt cttttccttc tttttggata
     3961 cgcaatgtct cttggattgt ttcgatcaaa tcattttgag tggtttgaag ggtctcgata
     4021 tccacgattc cacgttcatt ttctttcgct gtttcgatgg ctgagatttt cagcatttct
     4081 gaatttttct tcaataagtc gtttgttgtt tcagagactt ggcgttgagc agttactgca
     4141 tctttttgac gaagcaaggt caatgcgatc acgacttggt ttttccacag cggaatggct
     4201 gtagcgatag atgcttggat cttttcagcc aaggcctgat ttgtgttttg gatcaaacgg
     4261 atttgtggtg cttgctggat cgtgatctgt ctagctaagc gaagatcatg cgtacgttta
     4321 tccagacgat ctaggaattg cgtgtaatca ttggcaatct gaacatccat ctgatcgcca
     4381 gtttcttctg ctcttttcat cgcttctgga atgatcgttg tttgaagttc ttccattttc
     4441 aattctccag cagcgatata aatattcagt gcatcaaagt agtctttgtt tttctggtaa
     4501 agctgttcta acatcagatt atcttttagc agaccgtctt tttctttatc taatttcact
     4561 gaaatcttgt caatttgtgc accaatcttt tgatattttg ctgtcacttc atagatcgat
     4621 tgtttgacct tcccgaagac acgctggaag atgttccctt ctccggcacg cagttcgtca
     4681 ggattggctt cttggagacg atacatcagt tcagttaagg aatctcctac aggaccaata
     4741 tcttgtgctt gcacatgatt caacatcgat tgtgagaatt cacttaattt tgcttgggca
     4801 gccgatccgt agctgatgac agactgtgca tcattagcat cgatttttgc tgctaactgt
     4861 ttagcttgtt cttggcgttc cgccggtagt ttgtcaatta aacgagcagc tgtctgttgc
     4921 tcttggagct gatcaatctc cgcttgttgt gaagtggtca gcgtatctgt tggagttgaa
     4981 aacgggttgt tcaataaatc ttcaagtgta tcgttaactg gtgtaacatc ttttgtttca
     5041 gtcggtttat gatccataaa aaattcctcc ttgttgaatc tgttctcgat tcttacatga
     5101 atgtctctac tttagaaaaa gtactcgaaa cagccagaca tcatctgaat gttccgagtc
     5161 ttcttttatg aacaaaagag ttaatccttt tgttctggtt catatttatt atcacgcttt
     5221 aagctctgtt tcgcaacaga tatctctaca tcgatatcat ctagatcctc agcaacgaat
     5281 ttttgataat cttgtgcgat taaagctgac agctggtcga tgatctgcgc gctttcttct
     5341 agtttttcat aggtctgttt gtttttgatc tcatggtcat tgatctcatt atacttattt
     5401 gtcaaatcaa ctagattcgg cagatgcgta taaagaaact gacttgcttc atgcagtcgg
     5461 tttggttctt tcaccaattc tttgaataaa gcttttgctg ctttgactgg atcatggcgg
     5521 agatcgattg ctttaagttt ggctgtctgc tgcatattca tttgcaattg attgatctca
     5581 tcttttgttt gattcattgt ttgtcgaaaa aatgaaattt cattcgctgt cataccgctt
     5641 ttttcatagt gagtttcaag ttcgtcagtc agtgcaggaa tttcttcttg ttttcccttt
     5701 ttcctgcgcg aaccaaagag tccccacaaa actaaaacta cacctatcaa taaaagtcct
     5761 agaacaaaag aaaaactatt gtttcggaca aagagaaata gcaagaccaa tgctagaagc
     5821 cacggccaat ttcgttttat cataataaac cctccatgtc atttatcgtt gagtattgaa
     5881 ttcttttcgt tattcgatta catacttatt ttagcatacg gactactaga aagagtatcg
     5941 gactaaagaa ggattttcta tatttagaga aaatctaagg tttatctatg gtaaactaat
     6001 aaggagtttt tgagaaaaat tgaaaaatga aaaaaaagca gttatgatta caaagaaagc
     6061 aggagaagat atgctgaaca atgagcaatt tgaagaaaaa acaatcagtc gcaaagaaat
     6121 ctatagcgga aaaattatta atgttgctgt agacgaagtt cgtttgccag atggaggaac
     6181 ttcgaagaga gaactggtat ttcatcctgg cggtgtcggg atcattgctt ttgacgaaca
     6241 agatcgtctg cttcttgtca aacaatttcg aaaaccttta gaaaaagtga ttttggagat
     6301 tccagctgga aaaatcgatc caggagaagg acaaaatccc gaaatgacag cagctagaga
     6361 attagaagaa gaaactggct atcgttcgaa gtcgctgagc catcttactt cgatgtatct
     6421 ttcaccaggt tttgcaaatg aagtccttca tatttatcat gcacaagggg tagagaaggt
     6481 cgagaatcct ttagcacaag atgaagacga agtactggag ttgtatcatc taactcttga
     6541 agaggcgcaa caagcgatga aagatcagtt gatttgtgat gcaaagacga tttatgcgat
     6601 tcaatactgg gaactattga ccaaaggaaa gtagagggat cggcttatgg ccaaaggacc
     6661 gcttattacg cgaagcgaac ttcgcaagcg acaacaagca caagcaagcg agtcattaaa
     6721 aaaacagcga aaagcagaaa cagcttatca gcaagaagaa aaaaagattg ctagctttta
     6781 tcgtaaagaa agcaaaaaga ataaaccaat cacgaaaaca aggatcagcg aacgggaaaa
     6841 gacgaccaaa tggaactctt ttttgatgaa atctcttatt attgttatcc taatgttatg
     6901 tgtggttttt ttagcaatcg catttatata gaaagaagga attaaaaaga tgaaaatcgg
     6961 tattatcggc gcaatggaag aagaagtaaa aattttgaga gagaacctaa gcgaaccatt
     7021 atcatgggaa cgcgcaggcg ctttatttat ttcaggttca ttaggtaacc acgaagtgat
     7081 cgtcgtacgt tccggtatag gaaaagtctt agcatctatt accacaagtc tgttgatcca
     7141 acaatacggc gtgaacatgg tgatcaatac aggttcagct ggcggaatcg gtgaaggctt
     7201 acaagtaggt gacatcgtta tatcagacaa agtagcttat tttgatgcag atgcaacagg
     7261 atttggctac aaaccaggac aattaccagg aatgcctcta tattatgaag caagcacata
     7321 tttgcgcagt gaaatggtta aagcagcaaa agcaacgaat ttgaacgcaa aagaaggatt
     7381 gatcgttaca ggtgatacat ttgtcgactc gccagataaa atcaaagaaa tcttaacgaa
     7441 tttccctgaa gcattagctt gcgaaatgga aggagccgct gttggacaaa cagctcgtca
     7501 gttcaatatc ccatttttga tcgtacgtgc aatgagcgac acagcagatc actcggcgac
     7561 acaaagcttt gacgaattta tcgaagacgc aggcaaacga tcagcagaaa tggtgatcga
     7621 atttgtcaaa catctcgtat aaggaggact aagatgaacg cattgatctc gattgactac
     7681 acatatgatt ttgttgcaga ggatggaaag ctgactactg ggacagcagg acaagtaatt
     7741 gaaaaaaagc tgactgctca tacaaagaag tttattgatc aaaaggattt tgtcgttttt
     7801 gccattgatg cgcatgatcc aaaagattcc tttcatccgg agaaccgctt atttcctccg
     7861 cataatgtga taggaacggg tggacgggaa ttgtacggct ctttaaaaag tttataccaa
     7921 gactatgagc aagaggaaaa tgtttattgg atagacaaac ggcattattc cgcttttagt
     7981 ggaacagatc tagacatctg tttgcgagaa cgccagatca cagatattta tttgaccggc
     8041 gtatgtacag atatctgtgt gttgcataca gcagtcgatg cgtataatct tggatacaaa
     8101 ctccatattt tcaaagatgc cgtggcaagt ttcgatcccg ttggacatga atgggccttg
     8161 agacattttg aatccacact aggcgcagaa attttataaa aaaacaaagt gatttgataa
     8221 aataaaaagc acaaaataat aaaagaagag cataaaacaa acgactttgt tttacgctct
     8281 tttttatacg ttattttaaa aaaatattat tctttttacg ttaataaatc tctttattat
     8341 caacaactta agttatattt tttcttttta taaaatacac ttattataat tttgtttttt
     8401 attgacagtg aaaataaatt aggtaatact ttaagtaaac gctttctaat ttgttgcggg
     8461 aggaatacac ttgttaaaaa aacgtataaa atacagtgcg ctaatgatga atctccttat
     8521 gctcagtacc ccggttttaa tttctgtcgt tgaagttgct gctaacgaac aacagtctgt
     8581 gaacgaagaa aatgcacacg tgaacattga aggggtgtca acaatggagg aagtaccaag
     8641 aactagtgaa gatatggata cggaagaagg aaatgatcct atgatgaaag aatcagaaaa
     8701 tgaaaagaca aaaaagcaat ctgaagaagc aaatgaagag tggaaagggg ttgttttcgg
     8761 agaaagcaca agtgctagta ggtatgccat tgaaccatta gaagacggcg tgcggctctc
     8821 ttccacgaat aacggcggaa aattccagtc aagcggacca gatggaatga cctattatta
     8881 tcaagcaatc cccaaagaca tcaactttag gatgcgggcg acgatcgaaa tagaatcttg
     8941 gacctatacg aatgcccaag aaggatttgc tttaatggta agagacgcag taccaaaaga
     9001 tcatttgttt gggcaggcat tttattcaaa ttcatttgcc atactaggca gtcggatcga
     9061 atatgtctgg gactcggata gacaagaagt ggtcaacacg agcggcagca a
//
LOCUS       DACEPW010000102         8920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEPW010000102 DACEPW010000000
VERSION     DACEPW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.103"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            263..658
                     /locus_tag="IW819_001858"
     CDS             263..658
                     /locus_tag="IW819_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244960.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            655..909
                     /locus_tag="IW819_001859"
     CDS             655..909
                     /locus_tag="IW819_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244961.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            952..1347
                     /locus_tag="IW819_001860"
     CDS             952..1347
                     /locus_tag="IW819_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244962.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            1347..2402
                     /locus_tag="IW819_001861"
     CDS             1347..2402
                     /locus_tag="IW819_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ1244963.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            2399..2605
                     /locus_tag="IW819_001862"
     CDS             2399..2605
                     /locus_tag="IW819_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244964.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            2617..2940
                     /locus_tag="IW819_001863"
     CDS             2617..2940
                     /locus_tag="IW819_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244965.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            2933..3199
                     /locus_tag="IW819_001864"
     CDS             2933..3199
                     /locus_tag="IW819_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244966.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(3362..5707)
                     /locus_tag="IW819_001865"
     CDS             complement(3362..5707)
                     /locus_tag="IW819_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1244967.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            5986..6888
                     /locus_tag="IW819_001866"
     CDS             5986..6888
                     /locus_tag="IW819_001866"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1244968.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            7100..8875
                     /locus_tag="IW819_001867"
     CDS             7100..8875
                     /locus_tag="IW819_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1244969.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         2932 a         1589 c         1793 g         2606 t
ORIGIN      
        1 ataacccctt ttttttgtga ctttaaaaaa agagaaaatg gcggaaacaa accttacgtc
       61 catcgtatac gacacaatct gtgcgtagat gctacatttt gttcactact atcaaaatta
      121 ctttaaaatg aaaacgtgat aaaaaagaaa gtagaaaagt agaaaagtaa ctctaaaaaa
      181 tccaaaaaaa tccaggctcg attgcgtact tatcttaaag gagaaacaat ccatcataca
      241 taacccgaaa ggagacttct atttgcctaa gaaaagacga aagaccatag tcatcatttt
      301 gaccattctt gctataggaa tcggaggaaa attttacatg gacaaaagag aagcacaaag
      361 acagaaagat ttactcgcag tagaaaaaca atccgtcaaa gttctgaaaa atacctttgc
      421 agatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttactg gatgttatgg
      481 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt attttttttc
      541 aaaacaaatg aatgaaatta gcagttatgg tgtagaaaat gaagaaatac aaaacgaagg
      601 ggttactact cctaaaataa gagttattta ttctaatgga gaggcggagg aagtgtgaca
      661 gtaaaagacg aagagtatag atatataagt gaacatgttt attggctaga tccaaaacat
      721 cctcaatata gtaaaagtta caaagaaggc gccattaaaa actttggaga aacaaaattc
      781 caaatcctaa aaatcaaaga ctcattggat ggcatgcagg cgatggctgc agcgccaatc
      841 gtccatagca aattggagaa aaatttcaaa aataaaaaaa ttcctacaaa ctttcgcgta
      901 cttaaataat gaaggtatca atacttacat aaaaagaaag gaggcacgtt cttgtctcgg
      961 aaaataagaa aactcagctt agccatttta ttaatcgtca tattaggaat cggagggaaa
     1021 gtctatatgg acaaaagaga agcacaaaga cagaaagatt tactcgcagt agaaaaacag
     1081 tcagtcaaag ttttgaaaaa tacttttgcg gatatcaagg aagtgaagat tgaaaaatct
     1141 gctcgaaatg aaatgacggg agcatatcgt atgtttgtga caatgataaa taatcacgat
     1201 gagtctgttt attttacgtt tggattttgg aaagaacgaa atgaattagg agattatggc
     1261 gtagaaaata aaagtataca aaaagagggg attacagtaa ataaaatcag agtaattttt
     1321 tctaataata aggaggaata tatttaatga tggcagacaa acaatacagt gttattagtg
     1381 aggctgttta ctggctagat cctaaacaca gagactatga tcatacttat aaagaaaatt
     1441 ctattaaaaa aattgatagt tataatttta aaatcctaaa aatcaaagac tcattggacg
     1501 gcatgcaggc gatgggtgta gcgccaatca agaacggtaa agtggatctt tctcaagtag
     1561 tgattgcttt tgcaggaacg aacttaagtg attggaaaga tttggaaacg gatgcaagga
     1621 gtatcatgaa tctctatggg aatagaccag gagcaagttt ttctgaaaaa gcaacaacaa
     1681 acaagatttt atccgttagt ggattgaaaa cagcggggca aatcaattca gcgattgcat
     1741 ttgctaatga agtaaaaaag gaatatggag aagctgctat ttctactacc ggccattcat
     1801 taggtggatt cttagcatta tatgtagcag cagagaaaca atggaaaaat gtcggcttaa
     1861 acgggccgga cccgtatgat ctcttatcac cagaagcaaa aaaatgggtg gaagaaaatc
     1921 ccggtatgct caccaattac cgaaatcacg ccgattttat cggtaatttt ggtggtaatg
     1981 gcacaggtgc tgaagttcgg atcagtatgg aaacagcatt cttgaatctg cttgcttccc
     2041 acagtttaac ctcttggaag tttgatgaaa atggaaagct gattattcct gaaaatgaaa
     2101 ataatgttaa agctcgaaaa caacagaaag aaaaccaagc gacccagttg ttttcctatc
     2161 agttgcaaga actgaacaac atacgtaaaa aactacgatt aagtggcggc cggctgacag
     2221 ttaatgaaca aatttactta gatgacacac aagctaggtt agtagtggaa aagttatcag
     2281 caaaaatgaa aagcgctatg gaagaagtta tccgcatcaa tcaaagagga atttttgagc
     2341 tagagaaaaa atggcatggc aagaaggctt gcggcaagca cgtgttgtcg ccccttcgtt
     2401 gactgaacag gaaatcatag aagcattagc tagtgttgtc gccacaaaag agaacttagt
     2461 cgattctgcg aaagaagtct atctgctgcg aatcaataag gcaagacgta tgggggaggc
     2521 ctttgataca ttggtgaagg agatccaaga caaaatcaat gaaatcgtga cacgagatcg
     2581 agaattagca cagcaattca attaaaggag aagtagatga aactagaaga attggaaaga
     2641 gattatcgaa gtgaacgaag acaattagaa gctcaagaag atgaattgat ccactttaag
     2701 aatcaaggat tacgattgat ggagcaagag gagcagcgac tgcatcatgt attaaagaac
     2761 ttagctttag ataatgagcc tctgtttcaa gcaagacgaa gttattcgaa ttatcaggag
     2821 cattatcaac aagccctaca agtagaacga cgaaaactct atcagcagca agacgaacta
     2881 gaacgttcct atcgtcaaaa aatcagacaa ataactatgg agaaatcatc ttatgaatga
     2941 atttttaatg caattactaa aagaattaga gaaaaatcta catcgatcaa atgtacgttc
     3001 agttggtatg gggatatctc agcaaatttg ggcagaaaaa cgattgacag atcaaaacca
     3061 ccaaatgact gcagaagaag ctgctcaaga attttctaac cgacatgtac acttgaaaga
     3121 atcagtaaaa gggcaatttg cagaaaaact aaccgaatta ttccaaagac aagaaaagaa
     3181 attaaaacag tttcgttagt aaagttatat agaaaaattt tgtgaaaatg gcttttcaca
     3241 tttttacata aatttgtctt aatatcgtag atgcaagctt ttgaacacca tttattcgga
     3301 aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt aatctattag
     3361 atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca taagctgaag
     3421 cggtgttcca gtaatctgta tagttttcat cagtaccgcc aacgccgaat ttaaaggcgc
     3481 ttttcttcgg accatttttt gcccaaagtg attccactgt ttttcctggc gtttggatag
     3541 attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat acttttttct
     3601 tcatcacatc agatgataag tcaaatttct catcagtgcc gaaaatctca gggtttgctt
     3661 ggtaagcttg attgattaga ttggcaagat aattagccga acgtatcctt gcttggctgt
     3721 ccattccagt attgtcatcg tgtcctgccc agctactgat agtgatagac ggagtagaga
     3781 cgatcagcca tgaatctctg tattcgttgg tagaaccagt ttttccgacc caatctgctt
     3841 ttcctagatt tccgtttaag cttgaaatga catctttgaa tggtgtagtg attttttcat
     3901 tgatcacact gcgcatcatg tcattcatga tcgaggccgt tgcttcggaa taaatacgga
     3961 cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg gaatcaatga
     4021 tatagccttc ttcgtagacg ccaccattag ctaaggcctg gaatccattt gtctgttgaa
     4081 gcgtcgttac atctactgtc cctaaaggag cagattcaac gccccaatta tcatttgctg
     4141 ggtagttcat cttcgccaag tagtgctcgt aagcatattg tttcgatcca cccgaattca
     4201 atacgtcttg gtataagtga taagctggga tattgttgga ccattccagt gactcacgga
     4261 cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctcg ccagcatttt
     4321 ctccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg acaagtccca
     4381 tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct tgacgttctg
     4441 tatcaaaggc gtggttattt tgattctggc tgtaattccg gccacccacg aatccataga
     4501 tacgtccagt cctattgtcc atcaatacat ttccagtctc cacttggcta ccagcaccat
     4561 catctaacat atagccgtaa ttttctacgc cggtttgcaa tgcagcatag atatctttat
     4621 cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct ttttgataat
     4681 aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc aattgttgtg
     4741 caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg tcagtctgtt
     4801 ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct tcttcgtatt
     4861 gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct tttctggcaa
     4921 gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtagggacta tacgtgatag
     4981 ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatct tttgcatcta
     5041 caccaaagat tcctcttgca gcttcctgga ctccagcaat attttgcccc ttattattcc
     5101 gaccaaatgg cgagacattc aaatacgtag caatgatctc atcttttgaa aagtattttt
     5161 ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt tcatcggtta
     5221 ggatctgctg tttgactaat tgctgtgtca aagtggatcc tccagacgag ccgatgccag
     5281 tagcttctga aactaatgct cgtaaaacag ctttagggac ataaccttgg tgtttatcga
     5341 agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc ttatcagatg
     5401 aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta tctgcataaa
     5461 caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt ttagtcggca
     5521 attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata cctagagctg
     5581 cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact ctaaaaataa
     5641 ccttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga gtatttttgc
     5701 gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca aataataaga
     5761 caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag ataaaacgtg
     5821 aggaaatgat cttttttatg aaagagttct taagcttttc tttagagaaa gtttcagtta
     5881 ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc ttaccaatat
     5941 ataggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt attaggctca
     6001 cacgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc agccagttac
     6061 ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag aaaagctatc
     6121 gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact aacaaatatc
     6181 gtcgttcatg cgccctatat cattaatttg ggaaatacga tcaaaccaga aatgtttcca
     6241 tttgctgtac aatttctgag agaagagatt gttcgtgcgg aagctttagg tgctactcaa
     6301 atcacccttc acccaggcgc tcatgtagga gcaggggcag aaggtgggat tgctagaatt
     6361 attgagggtt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc tttggagacg
     6421 atggcaggga aaggtacgga aatcggtcga acatttgagg aactagcgca aatcatcgat
     6481 ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac gaatgatgcc
     6541 ggatataata taaaagaaga tttctcaggt gtgatggaag agtttgataa ggtcatcgga
     6601 ttagatcggc ttaaagtact ccatatcaac gattctaaaa atccacaagg ttctcataaa
     6661 gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa caaaatcgtc
     6721 catgatccaa tgtttgacaa catttcgaaa atcttagaaa ctccatatgt cggtaaagat
     6781 aaaaagaaag cttacgcacc atatggaaaa gaaattgcca tgttcaaagc gcaagcattc
     6841 gatcccgaag tgtttcctga gttattagca gaagaagtca cactttaagt tttctatctg
     6901 acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag aaacaaagga
     6961 gtactttttg cagtcttttt catcatgcat cttctccccc ctctttttaa atttagacaa
     7021 acaaggtata ctggtagaaa agtaaaaaaa cgaaaagttt cacaagcaag tggcaaaacg
     7081 tgattggaag gacgaaaaga tgctgaacac tttaaaaccc ttgatcaacc aactaaaaaa
     7141 gaaacataca tactttattc ttggcatagc cgtattattt acaattattc ggttgtattt
     7201 atatagtaaa gcaatctatg gaacagatat gtcggcaacc tatgatgatc agctgctgat
     7261 tcactatgct caaaatattt tggatggtaa atggctaggg gagtacacga ctacgacact
     7321 ttcaaaaggg atctcctaca gcttgttttt agtacttgca cataaactgc atctttctta
     7381 tgcagttttg ttcggcatgt tcagtattct tgccagcata ttggttgcac tagcgtttaa
     7441 accgatcgtg aagaacaagt atcttctttt aagtatctat ttttattttt tgttttctcc
     7501 gattagtttt acacatgagt attccactcg aacgtatcgt aatgctctcg tgatcccggc
     7561 tgttgagatc attatcgctt gtttactagc tatttattac cgtaaatata gttcccgcag
     7621 aaaattgctg ccgtggtttt tagggttagc tgtttctttt cctttctttt ggttcattcg
     7681 cgaagattcc atctggcttc ttccttttac tgtagtcgcg ttaggcatca gtttcgttca
     7741 gatcttgcta ggacaagact tttctttcaa agaagcaaag aagttaaaag aacgaccatt
     7801 tctcctttcg aaacaagaat ggctgaaact ttcattgttt ttactccctc tagtcggttt
     7861 ctccatcgtc tatcagtgga tcgaatataa aaacgagaca acatatggta ttgccaccat
     7921 caatgaccgt tcgagtggtg aatttggacg tttgatgaaa cagctgatcc gaatagatga
     7981 cgaaacgaac ttaaataaag aaaacagtga gatatgggtc tcgcatgctg cattaaataa
     8041 agcattagac gcatctccta catttcatac tgtttctact cgtattgacg aattgtatac
     8101 gactcatgcg tggacccaag gcggaaaagt aaaagaatta ccaggagata tcattttttg
     8161 ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa aagaaaccaa
     8221 tgatttttgg aaaaaagtca atcaagaatt agaagaagcc tatcaaaagg gaactatcaa
     8281 aaagaaaaaa gaattctatc tgatgggatc aggggatgga aagacgatga atgatctgcc
     8341 aatcgtattt gcttttctga aatcaggata tgcctatgca tggcgataca aagatttcca
     8401 tcaaggaggg aattatagtt tcgggacgca agaacaagtc gaacaggccc aagaattatt
     8461 gaatcttcca ctaatggata attggatcga tagcagtgat ccgagaacac ctgatttcca
     8521 gttgacaaaa acagcaaaag tcgcgaatgt catgataaaa atttaccaag ttctggccat
     8581 tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa ccgtatttgc
     8641 aaaagctgat cgtgcctatt atagttcctc tttgattgta gtcaccggat taatactgac
     8701 acaattggtt tttcttttcg gtgtctcgtg gttttgttct tatgcgccag aaaaaaagga
     8761 ttatttttta agtgtctaca ctggcgcagg cgtgccatta acacaattca tcgtagtaac
     8821 aagcatgcta gttttcagta aattgaatct gaaaaaagct ttatcttcta aataaaaagg
     8881 gaactgtgac aaaagtcttg tcgcagttcc tttttccgaa
//
LOCUS       DACEPW010000103         8891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEPW010000103 DACEPW010000000
VERSION     DACEPW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.104"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1193)
                     /locus_tag="IW819_001868"
     CDS             complement(<1..1193)
                     /locus_tag="IW819_001868"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAQ1244970.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGK"
     gene            complement(1352..1576)
                     /gene="nrdH"
                     /locus_tag="IW819_001869"
     CDS             complement(1352..1576)
                     /gene="nrdH"
                     /locus_tag="IW819_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1244971.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            1977..2450
                     /locus_tag="IW819_001870"
     CDS             1977..2450
                     /locus_tag="IW819_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1244972.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            2447..4579
                     /gene="feoB"
                     /locus_tag="IW819_001871"
     CDS             2447..4579
                     /gene="feoB"
                     /locus_tag="IW819_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1244973.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            4581..4715
                     /locus_tag="IW819_001872"
     CDS             4581..4715
                     /locus_tag="IW819_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1244974.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            4809..5453
                     /locus_tag="IW819_001873"
     CDS             4809..5453
                     /locus_tag="IW819_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1244975.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            5640..6833
                     /locus_tag="IW819_001874"
     CDS             5640..6833
                     /locus_tag="IW819_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1244976.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            6826..8553
                     /locus_tag="IW819_001875"
     CDS             6826..8553
                     /locus_tag="IW819_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1244977.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2856 a         1564 c         1898 g         2573 t
ORIGIN      
        1 tgtttgccgt taatcacaga aggcgtttga acttgtaaaa tctctgaaca taaattactc
       61 atgatgatct tgccatcgat tggattctcg cggttcgctg tatcaatatt gattacgtaa
      121 ggatagccag attcttgttg taattttgag atttcatttt caagatcacg cgctttgatt
      181 ttttgtttgc gaatgtttgg atttgcaact agatgatcat actcagctgt aatatttaca
      241 taagagaatg ggacaccata ttctttttcg acactatatg gactaaataa atacatatct
      301 tcgtttttac gtgtcaattc ataaaattta tcaggtacga tcacgccaag agaaagagtt
      361 tttacgcgaa tcttttcatc tgcattttct ttttttgctg aaaggaaagc aataatgtct
      421 ggatggaaaa cgttcaaata aaccacgcca gctccttgac gctgacctaa ttgattagaa
      481 tagctgaaac tatcttcgaa taatttcatg actggaacga caccacttgc agcaccctca
      541 tatcctttga taggagcgcc ggcttcacga agatttgata aagtaatccc tacgccgcca
      601 ccaatacggg acaattgcaa agcagagttg attgagcgcc cgatagagtt catatcgtca
      661 gtgacttgag tcaagaaaca agacaccaat tcgccccgac gtttacgtcc agcattcaag
      721 aaagaaggtg tagctggctg atatcgttga tggatcattt cgtctgcaag agcaagtgct
      781 aattcttcat ccccgttcgc aaagtaaagc gcattgaagg ctactcgatc ttcataagtt
      841 tccagataag cggaaccgtc gttggttttc aacgcatatt gagaataaaa tttgtaagca
      901 gccataaaag atttgaaaga aaaatgttgc tcgtccaaaa attgatatag cttttcaata
      961 aatgatggtg aatacaattg aatgaattct gcttctatat attcttgatc gatcaagtaa
     1021 ttgatttttt ccataataga tagaaaagtc atcgtatttg gtttgacatt ttcctcaaaa
     1081 aaggcaacta aagcttcttt atccttattc aaagggattt gaccattgac tggccgattg
     1141 atttcattat tcagcttgaa ataactgaca tcttgtaaat ttttaagact catacgtacg
     1201 cccaaccttc atttcgtatt tgatgatgct tgattgcaaa tcattgcatt gtttttaagc
     1261 tttaaaaaag gatttggaag tttgcgacaa aaactggtgc tgtctcaagc ccttcaaatc
     1321 catgtatcac gttttgattc ttttagtcat gttagctggc aagtttacgc aactgatcag
     1381 gacggaatcc gataactgtt tctgcatcag aagtaatgac aggaacactt tggaatcctt
     1441 gtgctttcaa ccagtctact gcttcaggct gttcatcgat attgacttct tcaaaagcaa
     1501 tttgattttc tgttaaaaac cgtttgacca tcttacattg catacaatta tttttagaaa
     1561 agatttttac gttcatggtg agtgcctcct ctaacacatt cttattatct ataatagata
     1621 gtataaaact cttagtaaaa aagtcaaaca acaaacacta catattgtgt tgtggaaact
     1681 gtggaatact aagttttgtg tttttgaaaa aagagctgaa aacaagtatc gtgtgggaaa
     1741 atagcaaatt taatagagaa aaaattgtgg aaaactatga agaaatattt ttttctcatt
     1801 tttatctatc aatactaaag attcgctcat aatatcacaa gtataattga cattctcaat
     1861 taccgagagt attctatgtg agaatgattc tcatttattt gcgtaacacg taagaaaatt
     1921 agaacatata atgaatgatt ttaatacatg atttatttca caagagagga atcaatatgc
     1981 agaccttagc gcaggcaaag cagaatttcg tttatcaagt cgaacgaatc gaaacagaag
     2041 aaaaaacaaa acgccatttg caaaatatcg ggatcgttgc cggaagtaaa atcgtcgtcg
     2101 tcaatttatc aggcaacaat gccattttac tggtaaaaag tcatcgagtg gcagtaagtc
     2161 gggaaatact aggtcaaatc tttgtcaata aaaagactgc ttcctctgaa agctggactt
     2221 ctcttgatca attaaaagca ggagagactg ggatcgtcgt agcaatccac ggacaaggcg
     2281 cagtgaagcg acggttaatg gatatgggat tgacgcgaaa tgtaaaaata gttatccgaa
     2341 aactagcccc tctgggagat ccaatcgaac tgacagtgcg agggtatgaa ctgacgttac
     2401 gaaagtcaga ggccgaacta atcttgatac aaagggagga aaaagaatga atatcgcgtt
     2461 agtcggaaat ccaaacagcg ggaaaactag cttgttcaat ctcttgactg gttcgaatca
     2521 atatgtaggg aattggccgg gcgtaacagt agaaaaaaaa gaaggatcgt tgaaaaaaaa
     2581 tccgaacatt catattcagg acctacccgg catctattct ttgtctcctt atacaccaga
     2641 agaagtgatt gcaagagatt atttggttca ggaacagcct gaactgatca tcaacattat
     2701 tgatgctacc aatttagaac gaaatcttta tttaactacg cagttattgg atttagacat
     2761 tcctatgatc attggtctga atatgatgga tgtcatcaaa aaagaaggaa aaaaaatcaa
     2821 tctagaaaag ctttcctatg gtcttggtgt accagtttta ccaatcagcg tattgaaaaa
     2881 gcaaggaatc gaaaaactaa tcgaaaagat tcaggagtct gcaaaaaagc ggccttctat
     2941 tcctgttccc tgtacatatg atccacgttt agaagcagct ttacatgaaa ttgaagatgt
     3001 actaggagaa caaagcggca agaaacgctg gtatgcaatg aaatattttg aacgagatca
     3061 gaaggtaaga gaggattacg agatagcagt ttctcagcaa aaagagatcg agcaactgat
     3121 ccagttaaca gagaaactat tggacgacga tagtgagacg attctggtca atgaacgtta
     3181 tgaatttatt acccaactat gtacactctg tgttgtgtca aatgattctt tccaattatc
     3241 gatgagtgac aaaatcgatc agattgcaac gaatcgttgg ttagccttgc cgatcttcgc
     3301 atttgtgatg tggctgatct attatctggc tattcaaaca gtaggaacaa tggggacaga
     3361 ttggatcaat gacacattgt ttggtacatg gttaccagaa catgttggca gattgttagc
     3421 tgaatggcaa gtagcgggat ggatgcagga gctgatcttg aacggcatca tcgcaggcgt
     3481 gggagcagtg ttaggatttc taccgcaatt gattatattg tttttatgct tgagctttct
     3541 ggaagactgc ggatatatgg cgaggatcgc ttttgtcatg gatcgacttt tcaggaaatt
     3601 cggtctttca ggaaaatcct tcattcctat gctgattgct acaggatgtg gagtgccagg
     3661 cgtcatggcc agtcgcacga ttgaaaacga gcaagatcgt cgtttgacga tcatggtcac
     3721 aaccttcatg ccttgttctg caaagttgcc gattattgcc ttagtagcag gggccttttt
     3781 tcctcatcaa tcttgggtag ctccttctgc ctattttctt ggtatgactg caattatttt
     3841 ctctggaatc agcttgaaaa aaacacgttt gtttgctgga gatacagctc cttttatcat
     3901 ggaattgccc gcatatcatt ttcctcaatg gtcagcggtc ctaagacaaa cctatgaccg
     3961 cagcaagtct tttgttaaaa aagcaggaac gattatcttt gtttctagta tcgtgatctg
     4021 gttcagctct tcttataatt tttatgctca acctgtttct gaagaccaaa gtatcttagc
     4081 ctttttcggg cgaatcctgg cagttgtttt tcagccgctt ggttggggaa actggcaagg
     4141 aacagtcgca gcgattacag gattggtcgc taaagaaaat attattggca catttggtat
     4201 tttgtttggt catgcggaag tatccgaaaa tggcagagaa atctggcaag tgctacaaca
     4261 cacatatact cccgcagcag cttattcatt gttagccttc aatttacttt gtgcgccttg
     4321 ttttgcggca attggggcta tccatcggga aatgaatgcc aagaaatgga cttggatcgc
     4381 cataggctat caatgcggtt tagcttattt agttagtttt gtgatttatc agttgggaca
     4441 cttattggag ggtggacagg tagcgttagg gacgtatctt gctattttac tgatgatagg
     4501 actcgtttat gttttgttcc gtcagccaaa atccaagaac cagttttata ctatattgtc
     4561 attagaagga gaggaatagg atggcgacat tcttattaag tgtgttaatc tttggaagtg
     4621 caggcgcaat tgtctatact cgtttaaaat ccggtaagaa ttgtgatgac tgtcaaacag
     4681 cttgcccagt aaaaaaggaa cagatcaagc gatgatcagt cgttgcaaga ggctattctc
     4741 cccttttttg cttattttgc tagagcatag taagatgagg gaaagtaaaa aaagaaagag
     4801 gaaagaaaat gtctggaaag aaaaaaatcg tccgcccgcg ctaccagcag attgctgttg
     4861 atttagccga acgtatcgtt gaaaaccgat ataaagtggg tgagaagatc catgcccgct
     4921 caacacttgc cagtacgtac aatgcttctc ctgagactac ccgcaaggct atcaatgtgc
     4981 tggtagatct tggaatcatg gaagtacggc atggaagtgg ggcatttgtt gcttcgaagg
     5041 aaaaagcaaa agatttccta ttgcaatatc aagatgtaca atccatccaa gatacaaaag
     5101 cagaaatcat ggcaagtgtg gagaagcaac aagatgagct gaatcatttt tctcaattat
     5161 tggatcagct ggtcaatcaa acgaagcgaa tacataagat gaatccttta cttccgtttg
     5221 aattatcttt atcagaaaaa gcaatgcatc tggaccgttc aattagcgag ttgaatatct
     5281 ggcaggcgac tggcgccaca gttatcgcaa tttctcataa tgaagaactg cttgtttcac
     5341 ctggccccta tgctaaattt acggcagggg acatcgttca ttttattgga aatgaatata
     5401 cactacagcg tatgacaaac tttttttacc cggagcatca gcttgcagaa tgattgtgtg
     5461 aaatattaaa aaaatattaa cttggctaaa ccttttattt acaaagattt agccaagttt
     5521 ttttcagttt ggacaagcaa atttctttca tttttgacaa agtgactaca tgatgttaac
     5581 atagtgtggt cacttttttc agaaagaaaa atgaatttac ataaaaaagg agaatttgtt
     5641 tggtaaaagt agaagtaaaa catttaacaa agatatttgg taaaaagaca caagccgctt
     5701 tagatatgat gaatgatcac cagccgaaaa cggagatttt aaagaaaacc ggtgcaacgg
     5761 ttggtgtgta tgacgtgaat tttgacgtaa aagaaggaga aatcttcgtg atcatggggc
     5821 tttccgggag tgggaaatcc acattgatcc gtctattgaa tcgtctgatt gagccaactt
     5881 cgggcagtat ctatattgat ggagaagatg tatcgaagtt aagcaaagaa gaattgcgag
     5941 aagtccgtcg tcataaaatc aatatggttt tccaaaactt cggtctgttt ccacatcgaa
     6001 cgatcttgga aaatacagaa tacgggttag aagtccgtgg tgtacctaaa gaagaacgac
     6061 aagaaaaagc agaaaaagca ttagaaaatt ctagtttgct ttcatttaaa gaccaatatc
     6121 ctagtcagtt atctggtggg atgcaacaac gggttggcct agccagagct ttagcaaatg
     6181 atcctgagat cttgctgatg gacgaagcgt tctcggctct tgatccgctg atccggcgag
     6241 aaatgcaaga cgaattactg gacctgcaag ccaatgttca aaaaacgatt attttcatta
     6301 cccatgattt gaatgaagct ttacggattg gggacagaat cgccttgatg aaagacggcg
     6361 aaatcatgca gatcggaaca ggtgaagaaa tattgaccaa tccagcaaat gattatgtac
     6421 gtgaatttgt ggaagaagtc gatcgttcca aagtgcttac ggctcaaaac atcatggtac
     6481 ctgcattgac gacgaatatc gaatctgatg gaccaaacgt tgcattgaca aggatgcgta
     6541 atgaagaagt cagcatgctg atggctgttg acagaaagag acacctgaaa gggatcatca
     6601 ctgcagatca agcgttagaa gctaggaagc aaaaacgtcc attgatcgac ttcctagatg
     6661 aaaatgtgac ggtgattggg aaagacatgg tggtaagtga tattttcaat attatttatg
     6721 actcaccgac tccacttgca gttgtagagg atggaaaatt aaaaggtgtc gtcatccgag
     6781 gtagtgtgat tgaagcatta gcagaaacaa gcgaggtgag tgaacatgaa taatctattg
     6841 aactatcaat taccagtggc aaactgggta gaaaatatca cagaatggtt tacgacaact
     6901 ttttctggcc tattttcatt tttacaaacg attggacagg cagtgatgtc ggggattaca
     6961 aatttactgt tagtgattcc agctcctttg tttatcttgt tattgacggt agcagctttc
     7021 tttatttcta agaaacgtcc aggacttacg ttgttcacat taatcggtct atggtttatt
     7081 tataaccaag gtctatggaa tgatttgatg aatactgtca cacttgttct tttatctagt
     7141 gtgatctcga tcatcattgg ggtaccatta gggatcttga tggcaaaaag cagtaaagcc
     7201 caaagtatta tcaaaccgat ccttgacttt atgcagacaa tgccaggatt cgtttatttg
     7261 attccagcag ttgcattttt cggtatcggg atggttccag gggtatttgc ctcagtcatc
     7321 tttgctctgc caccaaccgt tcgttttacg aatttaggga ttcgacaagt tcctaaagaa
     7381 ttagtagaag cttctgattc attcgggagc acaagtcgtc aaaaactatt caaactagaa
     7441 ttgcctttag ctaaaagtac gatcatggca gggatcaatc aaacaacaat gctgtcactt
     7501 tcaatggtcg ttattgcctc tatgatcggt gcacccggct taggacgcgg cgtattatca
     7561 gcattgcaaa gagcacaagt cgggaatgga tttgtcaacg gggttgcatt agttatctta
     7621 gcaatcatca ttgaccgttt tacacagcat ttgaaccagc caaacaataa aaaagctgca
     7681 ggagcaacaa cacagaaaag taaaaaacgt caaggactga tcattggtgc tgtagtagta
     7741 gtgatcttag gtgcgattgg tatagggagt ttttcatctg ccaaagaaac aaaacgtatc
     7801 aacttatctt atgttgaatg ggatacagaa gttgcttcaa caaatgtagt cggtgaagta
     7861 ttgaaacaaa tgggctatga tgtaacgatg acgccattag ataattcaat catgtggaaa
     7921 tctgtttcaa atggcgagtc agatgccatg gtttcagctt ggctacctaa aactcatggt
     7981 tcacaatatg cacaatataa agatcaagtc gaagacctag gtgccaattt gacaggagca
     8041 aaagttggtt tagcagtgcc agcctacatg gatgtaaatt cgatcgatga gttaaccgat
     8101 caggcaggta agaaaatcat aggaatcgag ccaggagctg gtgtagttac ggctgctgaa
     8161 aacacgatcc aaaaatatga caacttgaaa gactggaaag tagagacatc ttcttcagga
     8221 gcaatgaccg ttgcactagg tcaagcgatc aaaaaacacg aaccaattgt agttacagga
     8281 tggacacctc attggatgtt tgccaaatat gatctgaaat acttagaaga tcctgaaaac
     8341 ggaatgggaa gcgaagagca gatccatacg atggttcgaa aaggattgaa agaagaccag
     8401 ccagaagctt acaaagtatt agacaatttc cattggtcag aaaaagatat ggaaaaagtt
     8461 atgttggaaa tcaacaatgg aaaagatcca cagcaagcag caaaagactg gatcaaagag
     8521 aaccaagaat tagtcgaaag ctggaaaaaa taaaaagaca gacacgaaat aaatggagct
     8581 tccaaaattg gatcaaatat ccaaatttgg aagctccatg ttcgtctcta tcttttttgt
     8641 ctataaaaaa agagaatgcg tagctcatct tcgaaacgtc caaagacgcc gacaaactat
     8701 tatgaattta tgggtgcgag tcaaagagat cgatcgtagg tatgaaagac taatgtgatt
     8761 aaagtttatt atgtacgaag tgcattctca aaaaatatta aagaatatac atagtttata
     8821 tacttaaaag tgattaaatt cttattcagg aattattttg ctacgtttca agaaggaatt
     8881 gttttttgaa a
//
LOCUS       DACEPW010000104         8629 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEPW010000104 DACEPW010000000
VERSION     DACEPW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.105"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            242..1378
                     /locus_tag="IW819_001876"
     CDS             242..1378
                     /locus_tag="IW819_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1244978.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSSEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1515..1859
                     /locus_tag="IW819_001877"
     CDS             1515..1859
                     /locus_tag="IW819_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1244979.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2130..3416
                     /gene="efmA"
                     /locus_tag="IW819_001878"
     CDS             2130..3416
                     /gene="efmA"
                     /locus_tag="IW819_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1244980.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3554..4153)
                     /locus_tag="IW819_001879"
     CDS             complement(3554..4153)
                     /locus_tag="IW819_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1244981.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELITQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4557..5678
                     /gene="mnmA"
                     /locus_tag="IW819_001880"
     CDS             4557..5678
                     /gene="mnmA"
                     /locus_tag="IW819_001880"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1244982.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGDMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5924..7345
                     /gene="arcD"
                     /locus_tag="IW819_001881"
     CDS             5924..7345
                     /gene="arcD"
                     /locus_tag="IW819_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1244983.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7359..8477
                     /locus_tag="IW819_001882"
     CDS             7359..8477
                     /locus_tag="IW819_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1244984.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVNPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
BASE COUNT         2669 a         1472 c         1899 g         2589 t
ORIGIN      
        1 gaaaatacac cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa
       61 aaagagggaa agaagttgtg acacaagtga gcagcttcaa ggagctgcga caagactttt
      121 gtcacagctc cttttttcta tgcccatgaa acgtcctcta gacaaaaatg agctcttctt
      181 tggtttgttc gctattttat gctagaatgt gcctagcaat aatttggaag ggatgaatga
      241 agtgaaaact atttatctgg atcatgcggc aactaccccc atgcatccac aagtaattga
      301 agcgatgacg gcaatcatgc aagacacgtt tggaaatcct tcaagcatac atggatttgg
      361 tcgccacgcg catgaaaaat tggaaacagc aagacaaatc attgcagaca gtctgcaagc
      421 aaaacctcac gagatcatct ttaatagtgg tggaacagaa ggggataata cagctatctt
      481 agaaacagcg ttctcgcgtc aaaaagaagg cagacatcta atcactacag ctatcgaaca
      541 tcctgctgtt cttaatacca tgaaatattt agagacaaag gggtttgacg ttacatatct
      601 gcctgtagat gaaaaaggga atctgtcgat cgaagaggtc aaaaaagcgt tacgagaaga
      661 tacgatcttg gtctctgtga tgtacgggaa caatgaaatc ggaaatttga tgccaatcaa
      721 agaaatcggt gaacttttag cggatcaccc agcttatttc catacagatg cggtacaagc
      781 gtacggaaac caaacgatcc atccagaaga attaggaatc gatttattaa gtatctctgc
      841 tcataaaatc aatgggccaa aaggcgtcgg tttcttatat aaacgagacg gagtaaatat
      901 ccctgcattg cttttaggcg gggagcagga agaaaagcgg cgtgcaggta cagaaaattt
      961 agcagggatc tggggtatgg caaaagcagt tgaactgctg tcatctgaag aacgtgaagc
     1021 acgtaacaac aaatataaag gattccagca attgattctt gatacattag aagcaaacga
     1081 aatcccttat caagtaaatg gtgatttaac gaataaactc cctcatgtgt tgaatcttcg
     1141 cttaattggc gaagcaaatg atttgctact gatgaagctt gatcttaatg gggtcgctat
     1201 ttctactggt tcagcttgta cagcaggaaa tatcgagcct tcacacgtat tagaagcaat
     1261 gtatggagaa gatacaccga tactaaaaga atccatccgt atcagctttg gtttgggaaa
     1321 taccgaagac gacatccagt atttccttga tcaattagtt cgtgctgtca aaaaataact
     1381 agtgaagtca ttcttatatg aacagatgtc tgagagaacg ccttcatttt ttagggaact
     1441 taactgaaaa acctagtaaa aatcaggata aacttttata ataaagagaa actaatttta
     1501 tcgaggtgaa aatcatggca tttgaaacaa cagcaacggt actgggcgca ccaattagct
     1561 atcgtttgca tcctgatgca aaaagatata cattgcgcga taatggattt acagaaacaa
     1621 acggaggcaa ttatcaattg atccgaccat tagatgccac gccgcaaagc aaagaaggat
     1681 tcaaattaaa aatcactgta tcaaaagata ttcaaacatt gaaaatgtcg atcactacag
     1741 caaatggatt gaaagcagtc aatattttca aagatccaaa acagaaaatg agccaagata
     1801 aattttattt tctgatggat gggatgatta gccgtgggtt attcgaaaaa gtcgaatgat
     1861 ctgtggatat aaaaagacaa ctagttttga aacggcctat tgttagatga ctaactttgt
     1921 aaggacgacg caaattagtt gtctttttta tttttaaatt taacacatgt tgttattttt
     1981 aatattttgc tgatttattg tttctaagta aagaaattta aattgaaacg attgttattt
     2041 agggaaaaag gtattttttg aaaaaagaat cccgtataat gattgttgtg caaacgctat
     2101 acaaaatgaa aaagaaaaga gggatgttta tggaaaatga acagtcggtc gttttaacga
     2161 attggaagag aaattatttg ttctttttat cgggacagtt tttgtcagga atcactagta
     2221 tggtcgtcca atatgcgatc atctggtatt tgacaagaga aaccggttcg gcgacaattt
     2281 tgagttttgc tactttacta ggaatgattc ccatggtttt actaagccca tttgtaggac
     2341 cgctagtgga tcgctgggat aaaaaagctc ttttgattgt cacggatatt attgtagcga
     2401 ttttcgcgct tattttagca gtagtcggaa cgatttcaga atccttcccg atttggctag
     2461 tttttgtgtc tttgtttatg cgttcggtcg cacaaacatt ccaaatgcca acgatccagt
     2521 cgattatgcc gacaatcgtt ccatcttctc acataacaag gacgaatgga cagttaggaa
     2581 tggtccagtc agcgaatttc atcattgcgc cagctctagg tgcggctttg ttttcagtcg
     2641 ttcctgtcaa ttaccttatt ttattagatg tattgggggc agtttttggt gtcggcttat
     2701 tgatttttgt gaagatccca aaagtttctc ctgaaatatt ggaagttccg cttactattt
     2761 ttaaagatgc aaaatttggg ctgcagcaat tgatggataa caaagggtta tggtacataa
     2821 cgatcaatgg tgcatttgtg atgctgttgt tcatgccagc aatcagtctg tatccattga
     2881 tgacgctaga ttactttggt ggttccgttg gacaggcggg agcagtggaa gtagtctacg
     2941 cagtcggaat gctcttaggc ggtgcactga tcagttttat aggaacatgg aaagaccgaa
     3001 tgaaacctat tattatagcc tacatcatta tgggcctgac gatcggtgca agcggattgg
     3061 ttccaaacga tagtcaaggg ttcttgtact ttcttatctt gaatgcagga gcaggttgtg
     3121 caacgccata tttcaataca ctgctgatgg caatgatcca gcagagttat gaatcgaatg
     3181 ttttaggtcg tgtcttaggg aacttcaact ctttgatgaa cctggcaggt ccaattggat
     3241 tattatttgc aggaccatta gctgatcgtt taggagtaga aaaaatgttc ttgttttctg
     3301 gaatcgggat tttattatgc ggaatcgttt tatttttaac gtcagctgct cgaaattatg
     3361 ataaagaatt acagaaaaaa ttggtaaaag aacaccatga gcaaaaagac gaatagtatg
     3421 gaatggagtt gtgacagaag tcttgttaca actccatttc tgatcctaat gttcctgtgg
     3481 caataagcct actcaaatga aaaatatgag taattatctt tatttgttta atacttgata
     3541 tagggcagtt ttttcacgac ctatcgtact tcctctttgc ttcatagatt tctttgattt
     3601 gttgctcaga taacggagtg ccaaatggta gtttttcctt taagagttct ctttccggtg
     3661 cgtctacgcc tacattgaca tcgttctcga taggtacgct gtaatgatgg atatgtccat
     3721 gaagattgcg gatagtgtcg ttaggcccga gcaacatcgg ataatgagtc aagtaatatt
     3781 gttgatggtc gaatttaacg atggcaccta catcataaaa ataaaatttg ggacttccgt
     3841 cagtaagcct aggatcattt ttttctaaat aacggaaaaa agcacgactg tcatggttcc
     3901 ctttgatcaa gtggattgtt ccttgcagtt gagtgatcag ttccaaaatt tctgcatttg
     3961 ctttttcata ttgcggatgc atagcaaggt cgcctagatg ataaacatgg tcggtttctt
     4021 ttattactgc attccagcta gtgatcaact gttggttcat ttcatccaca tttgcaaaga
     4081 gccgcggagc aaagtcgttt tttcctaata agcgttcatg gaaaaagtgc atatcactgg
     4141 taaagtaatt catgtatctc ttgcctccct tttttcttag agtaaacaaa atcacttatc
     4201 tgagcaactc ataacatcta aaaagaaaag gttgtaaatg agacgtgcag ctttaagcga
     4261 taagaagcaa ttttgaaaaa tagtttttca caatttacaa aattattcct tattgccgaa
     4321 aggctggccc tttttatgag tatgccaatt ttttatatcg aatgctcaaa agatgagtga
     4381 ttttttggtt gactatcatg tttgtgtact gaataggtcc agtagaacag tatttttacg
     4441 acttgataat taagggatga aattttcaga aatatgacgt ataatgtaaa atactgaaaa
     4501 gttgcgacct tcaactcgta gatgtttcag aatctaattg aaatgatggt gatatcatga
     4561 cagacaacag caaaacacgc gttgtcgtag ggatgagcgg cggtgtcgac tcatctgtaa
     4621 cagcactttt gttaaaggaa caaggatacg acgtgatcgg aatattcatg aaaaattggg
     4681 atgacacgga cgaaaacggt gtatgcacgg ccacggaaga ttataaggat gtagcgaaag
     4741 tagctgctca aatcgggatt ccttattact ctgtgaattt tgaaaaagaa tattgggatc
     4801 gtgtatttga atatttccta gccgaatacc gcgcaggacg tacacctaac ccagacgtga
     4861 tgtgcaacaa agaaatcaaa ttcaaagcat tcttggatta tgcaatggat cttggcgccg
     4921 attatgtagc tactggacat tatgcgcaag tcactcgcga tgaaaacggc gtggtccaca
     4981 tgcttcgtgg agtagacaat aataaagatc aaacttactt ccttagccaa ttatcacaag
     5041 aacagctttc aaaaacgatg ttccctctag gcggcatgga aaaatcagaa gtacgggcaa
     5101 ttgctgaacg cgcaggcctt gcgactgcta agaaaaaaga ttcaacggga atctgcttta
     5161 ttggtgaaaa gaacttcaaa caatttttga gcaactatct gccagcaaaa aaaggagata
     5221 tggtcactct tgatggtgaa gtcaaaggac aacatgctgg cttgatgtat tatacgattg
     5281 gtcaacgtca aggactaggg atcggtggcg gaggagattc gcaagaaccc tggtttgttg
     5341 taggaaaaga tctagcgaca aacacattat atgtggggca agggttccat catcctgcat
     5401 tgtatgccac aagcttggat gcaagtgaaa tccactttac gacgaatgaa ccaatgccaa
     5461 aagaattcaa gtgtacagcg aaattccgtt atcgtcagca agacgttcct gtgacagttc
     5521 gcttattgga tgataatcgt gcagaagtcg tattcgacga acctgtacgt gcaatcactc
     5581 ctggacaagc agttgtattt tatgatggaa tggaatgtct gggtggtggt ttgatcgacc
     5641 acgcatatca agaaacaaaa gttttacaat acgtttaagc gatattaaat gaatcaaaac
     5701 agctatggct aacaaaagaa acaccttttc ttttggatag catagctgtt tttttgcaat
     5761 aaaattaatt tttattcatt ttatacttga aatacttgtg taaaaagttc ataatatcca
     5821 ataatatgaa taaagttgct ttttgttaag caagagcagt tgcaaaatag aagggaatct
     5881 tttgcaagtg atttgttcaa ttttataagt gagggtgatg gaaatggaaa agaaatcgac
     5941 gggtattagc aaatggggac tggttgctct ggttgtcagt tcgtcaatcg gaagcggtgt
     6001 gtttgggatc acaagtgatc tggctagttc tgcagctcca gggccagcga tcctttcatg
     6061 gataatcgtt gggattggta ttttagcttt ggtattatcg ctgaatcatt taggagaaaa
     6121 acgtccagat ctagatggtg gtattttcgg ttacgcggaa gcttcatttg gtaaattagg
     6181 tggcttcatc agtggttggg gttactggtt gtctgcgtgg ctgggaaatg ttgcctttgc
     6241 aacgatgctg atgagcgcca tgggagaatt tttcccgata ttcaaaggtg gacaaaatgt
     6301 tccttctatt ttatttgcaa gtatttttat ttggggtctg actttattag taaacaatgg
     6361 agttgaagga gcgagcgtca tcaatacaat cgtgacgatc tgtaaattag tgccattatt
     6421 cctattttta atatttgtat ttatcgcatt taaagtgaat atattcactg ctgatttttg
     6481 gggtaacgta tctgataatc tagtgaatgg aacagggcag caaggaacga tctggttgca
     6541 gattaaaggt tgtttgatgg ttatgatgtg ggtctttgta gggatcgaag gcgcaagtgt
     6601 tttagccaac cgcgcgaaga aacgttcaga agcccagcaa gcatcgatca ttggtctgct
     6661 ttgtttattg tttatctata tccttgcttc gatcttgcca tacggcgtct tatctcaaga
     6721 agagttagca actatttcac aacctgctat ggcaaatatt ttaaaaggaa tcgtgggaaa
     6781 ttggggagca gcgctgatca atatcggctt gattatttcg atcattggtt cttggttgtc
     6841 ttggacgatg ctcccagcag aaacaacgat gttgatggct cgagacggtc tgttgccgaa
     6901 aggttgggga aaaacgaata aaaaaaatgc cccaacttat tcgttagttg ttactgcaat
     6961 cttgactaat ctattcttat tgactttctt agttactgat tatgcctatc aatttgccta
     7021 ttctttatgt acagcggcaa ttttgatttg ttatctactt gtcgggatct atcaagtcca
     7081 gttttcttta aaagaaggaa tcaaaaaaca agtagtgatc ggtgggattg ctgtagtttt
     7141 tgaattgatg ggcatcattt tagcaggatt ttcttatgtc cttttatgta gtattgccta
     7201 tcttcctggt ttttgcttct actggatcgc ttgtcgtgaa accaaacatc aaattacggg
     7261 aaaagaaaaa gtaatgattg ggctgatttg tgtggccgca ctgctttcaa tcgggttgct
     7321 tgctgtaggc tggatcaaga tataaggggg aaagaaacat gaaaatcgca aatttcggtg
     7381 tagaagaatg gctaaataaa tgggaaaaga aagcggtata tgatattgca caaagctcga
     7441 tcgaagcatt gacattagac gaactcgttg gcttagatgg gactagcgta agtgaatttt
     7501 tcgaaaaacg caaaaacgaa ccattggact atggctggat cgaaggatca gatgttttca
     7561 aacaagaagt cgcttctctt tatcaaaccg tcaatccgga aaacatctta caaacgaatg
     7621 gagcgactgg tgctaatctt ttagctctat acgcgttagt ggaagcagga gaccatgttg
     7681 tttcgatgct tcctacttat cagcaattat atgacatccc caaatcttta ggtgccgctg
     7741 ttgactttgt tcatttgaag gaagaagaag attggcagtt tgatttagaa cagctagaag
     7801 ccttagtcaa acctgaaaca aaaatcatct gtttgaatag tgcaaacaat cctacaggca
     7861 cattgctaga tcggaaaact ttagaaaaaa tcgcagaaat tgctcgtaca gtagatgcat
     7921 atgtattgat agatgaagta tatgcgcctt taacggataa aggtgaattc ttgtcgatcg
     7981 tagatgtcta tgaaaaaggt atagcaacca attccctatc caaaacctac tccattccag
     8041 gtattcgtat tggctggaca gctactggac ctgagttggc agaagttttt cgaaagtatc
     8101 gagactacac gatgatttgc ggcggagtcc tgtcagatga tcttgctgta catgcattga
     8161 agaataaaga aaaaatattg gagcgaaaca aaaagatcat tacagagaac cttgtgattt
     8221 taaaacaatg ggtagccaat gaaccgaaag ttgaacttgt cgctcccaat tacgtctcta
     8281 cttctttcat taaattagcc atcgaagaag atgaccagac attttgtatc aatttattag
     8341 aagaaacagg agtactatta gtaccaggaa gtgcctttga tttaccgaag catgcccgac
     8401 taggttactg ttgtaaaaaa gaaaccttgg aaaagggatt attcctctta tctgagttct
     8461 tgaaaaaaca ggcctaaaca aaaacactgc aaggacagtc tgtgacaact gttcaagcag
     8521 tgtttttcat ttacgcttat tgtctatgag aaggagagtg ccctgaagat atggagactc
     8581 ctgaagcttt ttcttctgca gtttcttgct gatggatttc ttgtgtatt
//
LOCUS       DACEPW010000105         8608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEPW010000105 DACEPW010000000
VERSION     DACEPW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.106"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(74..1030)
                     /locus_tag="IW819_001883"
     CDS             complement(74..1030)
                     /locus_tag="IW819_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1244985.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1134..1736
                     /locus_tag="IW819_001884"
     CDS             1134..1736
                     /locus_tag="IW819_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1244986.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1824..3323)
                     /locus_tag="IW819_001885"
     CDS             complement(1824..3323)
                     /locus_tag="IW819_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1244987.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3339..3863)
                     /locus_tag="IW819_001886"
     CDS             complement(3339..3863)
                     /locus_tag="IW819_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1244988.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4161..4910
                     /locus_tag="IW819_001887"
     CDS             4161..4910
                     /locus_tag="IW819_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1244989.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4901..5686
                     /locus_tag="IW819_001888"
     CDS             4901..5686
                     /locus_tag="IW819_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1244990.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5655..6371
                     /locus_tag="IW819_001889"
     CDS             5655..6371
                     /locus_tag="IW819_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1244991.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6473..7492)
                     /locus_tag="IW819_001890"
     CDS             complement(6473..7492)
                     /locus_tag="IW819_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1244992.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7511..8569)
                     /locus_tag="IW819_001891"
     CDS             complement(7511..8569)
                     /locus_tag="IW819_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1244993.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         2583 a         1753 c         1541 g         2731 t
ORIGIN      
        1 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
       61 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
      121 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
      181 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
      241 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
      301 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
      361 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
      421 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
      481 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
      541 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
      601 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
      661 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
      721 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
      781 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
      841 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
      901 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
      961 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
     1021 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
     1081 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
     1141 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
     1201 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
     1261 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
     1321 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
     1381 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
     1441 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
     1501 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
     1561 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
     1621 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
     1681 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
     1741 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
     1801 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
     1861 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
     1921 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
     1981 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
     2041 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
     2101 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
     2161 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
     2221 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
     2281 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
     2341 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
     2401 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
     2461 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
     2521 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
     2581 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
     2641 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
     2701 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
     2761 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
     2821 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
     2881 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
     2941 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
     3001 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
     3061 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
     3121 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
     3181 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
     3241 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
     3301 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
     3361 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
     3421 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
     3481 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
     3541 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
     3601 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
     3661 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
     3721 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
     3781 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
     3841 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
     3901 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
     3961 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
     4021 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
     4081 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
     4141 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
     4201 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
     4261 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
     4321 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
     4381 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
     4441 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
     4501 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
     4561 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
     4621 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
     4681 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
     4741 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
     4801 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
     4861 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
     4921 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
     4981 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
     5041 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
     5101 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
     5161 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
     5221 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
     5281 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
     5341 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
     5401 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
     5461 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
     5521 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
     5581 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
     5641 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
     5701 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
     5761 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
     5821 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
     5881 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
     5941 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
     6001 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
     6061 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
     6121 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
     6181 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
     6241 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
     6301 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
     6361 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
     6421 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
     6481 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
     6541 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
     6601 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
     6661 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
     6721 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
     6781 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
     6841 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
     6901 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
     6961 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
     7021 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
     7081 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
     7141 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
     7201 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
     7261 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
     7321 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
     7381 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
     7441 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
     7501 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
     7561 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
     7621 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
     7681 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
     7741 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
     7801 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
     7861 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
     7921 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
     7981 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
     8041 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
     8101 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
     8161 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
     8221 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
     8281 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
     8341 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
     8401 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
     8461 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
     8521 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
     8581 taattatgtt catttattca caataaaa
//
LOCUS       DACEPW010000106         8516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEPW010000106 DACEPW010000000
VERSION     DACEPW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.107"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1500)
                     /gene="recJ"
                     /locus_tag="IW819_001892"
     CDS             complement(<1..1500)
                     /gene="recJ"
                     /locus_tag="IW819_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1244994.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQ"
     gene            complement(1642..2076)
                     /locus_tag="IW819_001893"
     CDS             complement(1642..2076)
                     /locus_tag="IW819_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1244995.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(2086..2874)
                     /locus_tag="IW819_001894"
     CDS             complement(2086..2874)
                     /locus_tag="IW819_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1244996.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(2871..3812)
                     /locus_tag="IW819_001895"
     CDS             complement(2871..3812)
                     /locus_tag="IW819_001895"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1244997.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(3989..4576)
                     /locus_tag="IW819_001896"
     CDS             complement(3989..4576)
                     /locus_tag="IW819_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1244998.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(4797..6110)
                     /gene="obgE"
                     /locus_tag="IW819_001897"
     CDS             complement(4797..6110)
                     /gene="obgE"
                     /locus_tag="IW819_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1244999.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            6398..7408
                     /gene="gap"
                     /locus_tag="IW819_001898"
     CDS             6398..7408
                     /gene="gap"
                     /locus_tag="IW819_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1245000.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(7493..8428)
                     /locus_tag="IW819_001899"
     CDS             complement(7493..8428)
                     /locus_tag="IW819_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1245001.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2322 a         1948 c         1485 g         2761 t
ORIGIN      
        1 ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa ataaaaactg
       61 aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa ttcgctctac
      121 tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc tcatcaagtc
      181 aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga cattttcact
      241 tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata aatctcgcat
      301 ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc ctttagctga
      361 tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca ttattttccc
      421 agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat ggatatgatt
      481 ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg cttttctttc
      541 ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat caaacgtcgc
      601 aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta gtctgggagc
      661 aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc tttcttcaaa
      721 tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta atgaaacaag
      781 gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg ctactaaatc
      841 caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg ctactccgac
      901 tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa caatcgcata
      961 agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt ctacaccagc
     1021 ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag tgatgatcaa
     1081 ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat acccatgctc
     1141 aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg tttctttcat
     1201 taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga ttttttctcc
     1261 actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta tcagatacgg
     1321 atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt cctcatggta
     1381 acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac ggatcatttg
     1441 gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg cttgtctcac
     1501 tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa gcagaaagaa
     1561 agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat cttttgtacg
     1621 accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg gcgattgagt
     1681 tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt gtaactccgc
     1741 ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct gttcttcttt
     1801 tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct gagtcagctg
     1861 tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata caatcagtgc
     1921 accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg aagtgaagcc
     1981 aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa tcaaaactaa
     2041 aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact ttttgttaaa
     2101 aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt ttgatgcaac
     2161 ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc ggacgatcat
     2221 ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat agcttccgct
     2281 tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg tcgtaacagc
     2341 tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga ctgcaaattt
     2401 cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag aggcgatatt
     2461 aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg tcaaaaccat
     2521 cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga atgtaaggaa
     2581 gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc cgacttcttc
     2641 catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta ccttgaaagc
     2701 ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta caaatgccat
     2761 tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac agatttgtgc
     2821 gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat tcatgcctct
     2881 tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt cgtgttttca
     2941 aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata tctcgcactg
     3001 atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc agcttgatga
     3061 gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa tgtgctttca
     3121 tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt tctcgtatcg
     3181 cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt tccattgatc
     3241 gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta cagtggtcca
     3301 gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg atcatgttca
     3361 accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata aacagaaaat
     3421 tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag cggataactt
     3481 aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc ttttggcccg
     3541 taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag cccgggaagt
     3601 ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt tctcggacga
     3661 atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa ccaaacttca
     3721 ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt tgcaggaaca
     3781 cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt taagttcctc
     3841 tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg gttcaaatta
     3901 aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa cagcagcgaa
     3961 cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt tcattgtcgt
     4021 tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc gcatgatttt
     4081 cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag cgagtaattt
     4141 ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct tcatcagcaa
     4201 tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg aacacggtct
     4261 gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg gcaaaccatc
     4321 ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt aatccacccg
     4381 tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat tgatgtgtcc
     4441 cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga acagcatgta
     4501 actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa tttaaaatga
     4561 ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta ttttaccttt
     4621 aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg ctgtgacaaa
     4681 agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat cacttctgtc
     4741 acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt gcttgcctat
     4801 tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt cgcaccgcgt
     4861 gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg catcactgtt
     4921 tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc agataagatc
     4981 cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt gaatccatag
     5041 tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt tacttctaac
     5101 aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc agaaattgga
     5161 aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg ttctttgaat
     5221 ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat gatttgcgga
     5281 cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag atagtcttca
     5341 taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac tcgtgttctt
     5401 tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc ttcaatcaag
     5461 cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat tcctagatta
     5521 ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga aataacggat
     5581 agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc caatactttc
     5641 aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat ttccggtgca
     5701 ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc ttttgccaca
     5761 actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc cgcatctcgg
     5821 acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc gtgcatccct
     5881 ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg aaaatccatc
     5941 aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc accgtctccg
     6001 ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat tccgtctccg
     6061 cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat aattaagtcc
     6121 tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa aagtcttact
     6181 tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg tttaccttct
     6241 tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt ataatttttt
     6301 gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat tgaggatgcc
     6361 gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa
     6421 cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat
     6481 tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt
     6541 tgactctact catggtactt atcctggcac agttactgca acagaagact caatcgtagt
     6601 ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa
     6661 agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc
     6721 aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat
     6781 gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc
     6841 agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt
     6901 tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt
     6961 agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat
     7021 cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa
     7081 attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc
     7141 agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt
     7201 tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac
     7261 aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca
     7321 acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg
     7381 cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg
     7441 actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc gttcaatgaa
     7501 catattggct taaaaattgt tggactactt gctgatattt ttcaggatct gtcttgtatg
     7561 cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc gttgcacgat
     7621 agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt ataaagagca
     7681 ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa aagtagccag
     7741 cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga agactaaaca
     7801 tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa tcttcaatga
     7861 ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt gctgctccca
     7921 tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc actttgttta
     7981 tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc ccttcgcttt
     8041 ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga aacatctttg
     8101 cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga gctgtttttt
     8161 cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct tttgaagtga
     8221 tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc gtttctttgg
     8281 atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa ggcacaacag
     8341 cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct aaagcgacgc
     8401 ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct tttttcatat
     8461 aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt tataac
//
LOCUS       DACEPW010000107         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEPW010000107 DACEPW010000000
VERSION     DACEPW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.108"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(71..1024)
                     /gene="menA"
                     /locus_tag="IW819_001900"
     CDS             complement(71..1024)
                     /gene="menA"
                     /locus_tag="IW819_001900"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1245002.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1037..2140)
                     /locus_tag="IW819_001901"
     CDS             complement(1037..2140)
                     /locus_tag="IW819_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1245003.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2230..3165)
                     /locus_tag="IW819_001902"
     CDS             complement(2230..3165)
                     /locus_tag="IW819_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1245004.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3467..5401
                     /locus_tag="IW819_001903"
     CDS             3467..5401
                     /locus_tag="IW819_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1245005.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5858..6274
                     /locus_tag="IW819_001904"
     CDS             5858..6274
                     /locus_tag="IW819_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1245006.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6288..6854
                     /locus_tag="IW819_001905"
     CDS             6288..6854
                     /locus_tag="IW819_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1245007.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6877..7863
                     /locus_tag="IW819_001906"
     CDS             6877..7863
                     /locus_tag="IW819_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1245008.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8018..>8494)
                     /gene="scm"
                     /locus_tag="IW819_001907"
     CDS             complement(8018..>8494)
                     /gene="scm"
                     /locus_tag="IW819_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1245009.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2490 a         1638 c         1729 g         2637 t
ORIGIN      
        1 aggagctatt tttatttggc tgttcttatt acttgatgca gggcagtttt ttcacaacct
       61 cttcatgctg ttaaatggct tgcataatga ttgttagtaa tagccccaat acataactgg
      121 cattgaataa gaccatattt ttgatggaat agctgaaact gataggctgg ggtaacgttt
      181 gcttgaattg atccaaatgc ttcttcacaa ttggcaaggt caagaaggtg accaagatcg
      241 gccattcata gataccgctc aacaacccca ttaaaatcac gaaatagcaa gcatacatca
      301 atagctggaa taagactgtt cccgcaggac gtccgatata aaaaactagt gtatagcgat
      361 gattttcaat atcacggtca agatcccgca aattattagc caacatgata ttggcaatcg
      421 taaacaccaa gggtaaggaa gctaatacga ttgctgctat agcccaaaga tttcctacca
      481 aagcaaattg tccagatgaa aagttgactg tcagatcata gacatctgaa ccgaatgtat
      541 tgacataaac gaccatggca aaaattccaa ggcccatagt caccccactg aaaatttccc
      601 ctaaaggcat tcgagataaa ggaatcggtc cgaatgtata gaatactcca ataaaacaac
      661 agataaatcc catcaaaagt aaaagccaac ccgtattgat cgttagaata acgccgataa
      721 caaaaacaaa tgcaatcata gctaaaatca tgtttcgaat caatacagga gatagatttc
      781 tgcggccaat gacattttct tcatacttat atacttcaga atgagccttt tcataatcca
      841 tgtaattatt gatagcggta gtcgctaaat caaaaactag cattccaata aaaaataaaa
      901 aagtgtttag ccaatgtatc tcatgaaaaa aagcgataga gaataatacc cctaccgcaa
      961 aaggaaatac actagccacc tttgttcgta tttcaactac ttctaaaaag atttttagtg
     1021 tcatggttcg tactccttat tttagttcag aacggtcacc catcgtgtaa tgggagtcct
     1081 ccgataattt gaaggtgtct ttgattggat cggtcacata tactttatcg tcttttgtca
     1141 cgaagatcgc ttccgtcccg ttattcaatt cgctttcgac gtattccagt ccttttttca
     1201 cacccattga gaagactgct gtagacaacc cgtcaccatc aattgattta tctgttatga
     1261 tcgtgacact tgcgatatca ttgtcgaacg gataacccgt ttttggatca aacaaatgat
     1321 ggtatttttt gccatctact tctaaataac gctcgtaaat tccagaggta actaatgttt
     1381 tgttccgttc tttaatagaa cctaaaacag atccgcgagc catgttcgga tcttggatgc
     1441 cgactgtcca gtcttggttt tcgcctcttg ggctgtggcc taagacatag acattaccgc
     1501 ccaagtcaac gatggcagtg gtcactcctt gttttttcaa gactttcact acttcgtccg
     1561 taatgtaacc tttggcgatt gcacctaaat cgatgatcat tcctttttct tttagataaa
     1621 cagttttttc tttgtcattt agttcgatct tatgataatc cactaatttc aatgcctgat
     1681 cgatttcttc ttgggaaggt tttctggcgt catcaaaacc gattcgccat aattgtgtga
     1741 ttgcaccaat cgccatatcg aatccgcctt gagaatcttg gctatattcg taggcccgct
     1801 tgactaacgt gtagacgtca tcagaaactt ttactggttt gatcccagct tgctcattca
     1861 cttcatcgat ctcagaacct ttttgattga tcgtgatttt atcaccaagt tcttttactc
     1921 gatcgaaggc aggtttcaat gctgattctt tgccttcatc atacacacgg atacgaacgt
     1981 aagtccctaa caagaactgt tcatcagaat atggatcttt caatatcttg gtagctgctg
     2041 aactttcagt ggttgtttta tttgctgagt tgctgcagcc agttagcacc ataaaagaga
     2101 tgcccagtgt gattaaggaa aatagtagtt ttttccgcat ctttttcctc tcttcatttt
     2161 gaaaggtgag acgactgcct aatctggcta tcgtcccacc ctccaacttt tattagtatg
     2221 attatgtgat tatttttctg ttacgatatt atctacttcg attgtttgag tgtcgccttt
     2281 ttcagctgca ttcactaatt gttgtgcgta catgatgaat gaatgtgagc tgtgtgtagc
     2341 acctgtaaca acttctacat tagcaggtga gccatcttct tcgcccattg ctttgacgaa
     2401 ttcgtcgttc agagtaggga tgtaagtttt tggttctgta ccgctttttg ctttcatatt
     2461 ttcattatat tctgtatcat cttgttttga tttaccgtct ttgtcgatat aatcaaagtt
     2521 tgattcagtt actttgccgc cagcaactgt catttcgaat tgcacacggt agccgttaga
     2581 gtagttcttt tcttcaagct tgtaagtgcc gtctttcaaa tcagcaccat tgtcgatttc
     2641 gattgtatct gtgttaccag cttgagcagc ttggatcaat tgttgtgcgt agttttggaa
     2701 gctttcagat gagtgagtag ctccagttac gacttccaca ccgcttgcgc tttgtgcttt
     2761 taagaattgg tcattcaact caggaatgta tttttctggg tttgtgccag attttgcttc
     2821 catgtttttg ttgtattctg catcttttgt ttttgattcg ccgttttcat tgacgttatc
     2881 atattttgat tcagtgattt tgccgtcttt tacaaccatt tcaaagactg cacggtaacc
     2941 atttgaataa tttttttctt ctaatttata tgtgccgtct tgcaaatctc cgccagctac
     3001 aacttttgca gatgattctg ttgtgctttt tgcagtcgaa gattgtgctg tagatgattc
     3061 agaagaagag tttgagcttc cgttgtcatc tgcgccgcaa gcgcctaaca ctagagctga
     3121 aaatgctaat acggcaaatc cagttacaaa acgttttttc ttcattttaa taccccacca
     3181 ttccatttct agtccacaat ttttctttgt gattcattat acattctaat ctttttagcc
     3241 tataagtcaa ctatttttga taaataaccc atgtaatatt tcaaacgttt tctttcagca
     3301 aatttcgtga aaaaacgctt tattttaaaa tttgaaaaaa atccaaaagg agttagacaa
     3361 aagaacagaa catctatata atatatggag attgtgtaat aagtatcaaa ctaaaagaat
     3421 tatcatatat tcattatata gatagtctat aaggagcgga tgatcaatgg caaagcaaaa
     3481 tattgtcgtt gtaggagcag gatatgccgg tgtttcagct acaaaatact tggcaaagaa
     3541 actgaaaaaa gaagatgtag caattactct gatcgatcgt cattcgtacc atactatgat
     3601 gaccgagttg catgaagtag ctggcggacg tgtagaacca gaagcaatcc aatacgatct
     3661 gcagcatttg tttgctcgtc aaaagaatgt acaacttgtc acagacactg taatcggtat
     3721 cgataaagaa cgaaaaatcg tcaagaccaa attaggttcc tatccttttg atcagctgat
     3781 ccttggaatg ggcggagagc cgaatgactt tggtacacct ggtgtcaaag cacacggatt
     3841 caccctttgg tcatttgaag attctgtaag gattcgggaa catattgaga agacagtagc
     3901 aaaagctgca ttggaaccag atgatgaact gcgtaaagcc atgctgactt tcgttgtctg
     3961 tggttctgga tttaccggta ttgaaatgat tggggaactg atcgactgga aaaatcgcct
     4021 agccaaagat tataaattag atccaaacga aatcacactg atggtcgtag aggcaatgcc
     4081 gacgatcttg aatatgttgg atcgaaatga tgctgcaaaa gctgaacgct atttgcgtaa
     4141 gcacaacgtg gaattattat tgaatgcacc aatcgtagaa gtggctgctg atcatatcaa
     4201 attgaaagac ggacgcaaca ttccgactca cacactgatt tggacagctg gtgtcaaagg
     4261 aacttctgac gctgctgact ttggattaga atcagcacgc ggacagcggt tgatcgccaa
     4321 tgaatatatg caggcaaaag gctacgaaga taaaaacatc tatatcattg gtgatttagt
     4381 cttttatgaa gaattcccag gaacaccgac accgcagatc gttcaagctg ctgaacaaac
     4441 aggacacaca gccgcagcga atatcgtggc ttcgatcaaa ggcggagaaa agcataaatt
     4501 taaaggaaac tatcaaggat ttatggtttc tattggtgcc aaatgggggg tagctaacct
     4561 atttgataag atccatttaa gcggattttt agctatcgta atgaaacata tcgtgaattt
     4621 gaaatacttc tttgatattc gttctggtta ttatatgttc caatatttaa tgcatgagtt
     4681 tttccatatc aaagatgatc gcaacgtgac tcgaggacat tcttctcgtt acggcaatgt
     4741 cttatggagt gtgccacttc gtattttcta tggtttagtt tggttcgttg aagcttccaa
     4801 aaaaatcatt gggaatggtg aagtgttcaa accaagtact tggtttggtg aaggatcatg
     4861 gttcactgga aacagtgtgt tcccttggac atgggaatac aatgtaacag atgttgccag
     4921 tggtgcttct gctgctggtg atgcaaccac agctgccagc ggagcagggg caagtgccgg
     4981 agaagcagcg actcaagctg cacattttgg tttgagttat gcatacggtg aagaaccaat
     5041 ggctgtgatt gagaagtcac cagactggtt cacaaagatc atgcaagtga tcatgccaaa
     5101 caatgatgta gcaatgttct tccaaaaatt catggtattc tttgaaattg ccttggcatt
     5161 agcattgatt gctggattat tcacatggct atgcagtgcg acaacgattg tattggtgat
     5221 cacgttctgc ttatcaggca tgttttactg ggtaaacatt tggttcattt ttgttgcttt
     5281 cgcattgatg aacggttccg gacgtgcgat tgggttggat cgctgggtaa ttccatggct
     5341 acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt tcccagtacg gcaagaaata
     5401 aatagataaa gaacgttatt tgaatgcaag atttcaatag aaaggggctg agacaaaccg
     5461 tcacagcctc ctttttatgt aaaattaaag gaataagatg cccgagataa gcctccatga
     5521 gcaagacaca ggtgtcacaa cctctgttac aacctcttgt tataaacggt gttccagaag
     5581 tagcttcttc gaaaaataag ccgaaatttc acaaaaactt gaaaagtaat tttcgtaaat
     5641 tccttcttat ttcttgaagt tgcccacttc tgtcacaacc tcttgttata aacggtgttc
     5701 cacctgctgc ttctcggtaa taagcccaaa gaattagaaa acatggaaag ctgttttcta
     5761 ttcttcgttc ttattactca aagcaagaca caggtgtcac aacctcttgt ttttatgcta
     5821 aaattgggtg agttaatctg gaaaaaggag aacaaaaatg agccttaaag aatttgctga
     5881 aaaaagtctg ctcaaacctt gggatgtttt tattattttg cttttgactg ttctttcttt
     5941 tttaccagtc atgatttttg gtcttcagca aaacaccgca acagcagaaa aagaagcggt
     6001 actgcgtgtg gatggcacag aaatcaaaac attccctttg atggctggag aagagagcaa
     6061 cacatatact tacaaagatg aacacggaga ttataacttg atcgagatag atggcgatcg
     6121 agtacggata aaagaagcag attgcgatga ccagatctgc gtacgtcgag gctgggcaac
     6181 gaaaaatggc gaaaccatcg tctgtttgcc acataaatta gtgatcgaag tccgttcaac
     6241 agatggaggt gacgaagaca gtctgattta ttagaccttc acacaccatg agtaaattac
     6301 aaaaaatgat ttatatttcg ctacttgttg cacaaggagt gatcattggt ttattggaaa
     6361 atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa actaggctta gccaatctaa
     6421 tcacgatcgt tgccattttt accatgaaaa aacgagacag cttcttacta ctttggctac
     6481 gactattcct gactactttg ctaggcggaa cgatttccac attcctttat agtatgagtg
     6541 gtgctttgct tagttatttt ggaatgttat tggtcaagca attaggacca aaacgtgtca
     6601 gtatcatcgg aattagtgcg gccggcggtt tcatgcataa tgtcggacag cttttgatgg
     6661 cttctttcat tgcacaatca tggacagtca tgttgtactt gccagtcctt tcagttttag
     6721 ggattttatc agggattgca atcgggatcg cagcgaatta tcttttacga catgtgcaga
     6781 cacttcaacg attccaaaaa gattatgagc agggaaccaa acaaaaatgg agcccaatgt
     6841 ttaccaaaaa ataatcgtca cacaaggagt ttttttatga agctacatcc aatgtgggaa
     6901 aactatcctg ctttaagtaa agaattgaca gaaacactaa aacggatgga agaaactgtc
     6961 cgcctgaaaa acaaacctgt cgaacaagca atcaaagaaa cgatccatgc cggcggaaag
     7021 ctgttgcgtc ctgcctatca gctattgttc tcacaatttg gaccagagca agatcgagac
     7081 aaagccatcg ctttggcagc ttctatcgaa atgctccata cggccacact gatccatgat
     7141 gatatcgttg atgaagcgga tctgcgacgt ggtcagccta atatccgtag tcagtttggt
     7201 aatgtcgttt ctgtctatgc aggagactat ttatttgtct gctgttttaa actattgtct
     7261 gattactcca cttccttgaa aagtctccaa ttgaattctc gcagtatgga aaaagtattg
     7321 aatggcgaat tagggcagat ggatgatcgc tataattatg aactttctgt aaaagaatat
     7381 ttggaaaata tttcaggtaa gacagctgaa ctgttccaat taagttgttc tgttggcgct
     7441 tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag gagatatcgg actttctatc
     7501 gggatggcct ttcagattat cgatgatatt ttagattata caaaagagag ccaggaaatc
     7561 ggcaaacctg tactagaaga catgcgtcaa ggggtctatt ctttaccgtt gatctactct
     7621 ttgcaaaaaa acaaaccaaa gctactacct tatttagaga agaaagcggc aatgacagaa
     7681 gaagatgtcg acgccgtacg caaaatcgtt gaacatacag gaggcgtgga agaagctagg
     7741 aagttagcgg cctcttacac agaaaaggct ttgaaggaaa taaagaaatt acctgccacc
     7801 tctcttcgaa caaaagagaa tctcttttcc cttacccaac tgattttgga tcgcaaagac
     7861 tgaataggga aaggttcgat acgtaaataa atttctcaga aggaaatcta cgtgtttttt
     7921 gacaaattga attgtagttc ttgtatttat gataaaaagc aaaaataaag agcaacgtcc
     7981 gttagcgacg ttgctctttt ttatcttgaa tattttttta ccttttcgat ttcttataac
     8041 tcataaaagt gattcttaaa ccgattacaa gtaatataac gcctgcgagt gttatccaat
     8101 tattcgattg acttccagtt ttaggaagtg ctctttttga tttgtttcca ttgtcattgc
     8161 ttgttttatt agttaagtta aagttcgtcc tgttatccga acttgttttt gagaaggaga
     8221 cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc tgtactatcg cttgaagttg
     8281 atgaatctga gctatcactt gtggtagagg atgtatctgt actgtcactt gaagctgacg
     8341 tgtccgtgct atcacttgta gtggtagatg aatctgagct atcacttgtg gtagaggtag
     8401 tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc acttgttgtg gtagatgtat
     8461 ccgtactgtc gcttgaagct gacgtgtccg tgct
//
LOCUS       DACEPW010000108         8447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEPW010000108 DACEPW010000000
VERSION     DACEPW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.109"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            164..817
                     /locus_tag="IW819_001908"
     CDS             164..817
                     /locus_tag="IW819_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1245010.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            902..1807
                     /locus_tag="IW819_001909"
     CDS             902..1807
                     /locus_tag="IW819_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1245011.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1809..2441)
                     /locus_tag="IW819_001910"
     CDS             complement(1809..2441)
                     /locus_tag="IW819_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1245012.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            2761..3285
                     /gene="infC"
                     /locus_tag="IW819_001911"
     CDS             2761..3285
                     /gene="infC"
                     /locus_tag="IW819_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1245013.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3357..3557
                     /gene="rpmI"
                     /locus_tag="IW819_001912"
     CDS             3357..3557
                     /gene="rpmI"
                     /locus_tag="IW819_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1245014.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3610..3969
                     /gene="rplT"
                     /locus_tag="IW819_001913"
     CDS             3610..3969
                     /gene="rplT"
                     /locus_tag="IW819_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1245015.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4221..5558
                     /locus_tag="IW819_001914"
     CDS             4221..5558
                     /locus_tag="IW819_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1245016.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            5606..7735
                     /locus_tag="IW819_001915"
     CDS             5606..7735
                     /locus_tag="IW819_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1245017.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            7710..8399
                     /locus_tag="IW819_001916"
     CDS             7710..8399
                     /locus_tag="IW819_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245018.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
BASE COUNT         2733 a         1484 c         1825 g         2405 t
ORIGIN      
        1 gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt cttctttgac
       61 tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa ggttggagtt
      121 aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg ttttagcagg
      181 tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact ttgatagtca
      241 ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg caaaccctga
      301 acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa gcatcaaaca
      361 agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt cactgctttt
      421 ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt atgatgaact
      481 gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc cggatttatt
      541 agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac gtggtcgttc
      601 gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat tgaacagacg
      661 ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg atggagaccg
      721 ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga tcgaagaaaa
      781 attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc aaataaaggg
      841 taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag gaggatagag
      901 tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg tacagcaaaa
      961 cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg agatgctgac
     1021 ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg atgaagcgtt
     1081 actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg aaactagtcc
     1141 tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga ataccgagta
     1201 cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg tatacagttt
     1261 attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg aggaagtgaa
     1321 tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca agaatctgct
     1381 gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa aacaaaatgt
     1441 agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag aaaaggaaaa
     1501 agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga cgaacttagc
     1561 ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata acaacttgaa
     1621 tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa ctattgtgac
     1681 aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg gatggggaat
     1741 cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt ggcggcggat
     1801 tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag tgcttgctct
     1861 tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt gaagaaatta
     1921 gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt caactgattg
     1981 tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc taattgacaa
     2041 tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga agcaatacaa
     2101 tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt taatttacat
     2161 tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga aaaatcacat
     2221 ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg catcgtaagt
     2281 ttttggatat gactctctct taataccata tttttggcat cgagatagct ttggtcaccc
     2341 ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc tccctttgta
     2401 agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca cctcgttttt
     2461 ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct aatcattttc
     2521 agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt tgctatacta
     2581 tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat aatgtcactg
     2641 ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg taagagaact
     2701 cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat ttggaggtga
     2761 atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga gttacgattg
     2821 atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt gaatattgca
     2881 gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc agttgcgcga
     2941 atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga agcgcgtaaa
     3001 aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga cttgaatgac
     3061 ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa agtgaaagct
     3121 tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa agtcttgaat
     3181 cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa aatggacgga
     3241 cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga atgactaact
     3301 ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat atagtcatgc
     3361 caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt aaaggcggat
     3421 tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa aaacaacgcc
     3481 gtcaattgcg taaagcaagc atggtttcta gtggcgactt caaacgtatt cgccaacaac
     3541 tagcaaaaat gaaataagaa gtacaaataa cagaatctga agatttattt ttattaggag
     3601 gaattataca tggcacgtgt taaaggtgga acagtaactc gtaaacgtcg taaaagagtg
     3661 ctaaaattag caaaaggtta ctacggttca aaacacactt tatttaaaac agcaaaagaa
     3721 caagtaatga attcatacaa ctacgcatat cgcgatcgtc gtcaaaagaa acgcgacttc
     3781 cgtaaattgt ggattgctcg tatcaacgca gcagctcgta tgaatggctt gagctactca
     3841 aaattaatgc acggtttgaa agttgctgaa atcgacatca accgtaaaat gttagctgac
     3901 ttggctgtta acgatgcagc agcatttact gcattggctg accaagcaaa agaagctttg
     3961 gctaaataag ctaaacaatt ataaagagga aagaagataa cgaaaagtta tgttctttcc
     4021 tcttttttta tttacaatcc tccatcaaat gaaacaggac aataacgtat aagtctaaaa
     4081 tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct ttaatgaaag
     4141 cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta tcagaaaaac
     4201 aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta gttatctcat
     4261 tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc tttgccatca
     4321 tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca aacccggcga
     4381 tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg ccagctgagt
     4441 acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt gttctagtag
     4501 atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg gacaagccaa
     4561 gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg atgacaggaa
     4621 tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca ttaggtattt
     4681 cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc tttgctaata
     4741 tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg gctgaaagct
     4801 atacttataa tgactatttc caaattggta tgattgggat gattgtaagc ttagttgtgg
     4861 tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg gcagcaaacg
     4921 taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta ttaccagttt
     4981 taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca ggtatctggg
     5041 cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt cagtttgcga
     5101 aattatttac agatggcgca gtagatgttg caccaatggt tggtttcttg atgaccttag
     5161 cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt tttggagatt
     5221 ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca ccactaggat
     5281 ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct gtcgtattag
     5341 ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc acaacgattg
     5401 ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt tatacaaaag
     5461 taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc ggagcgatct
     5521 tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt cgataaaaac
     5581 gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa ttgatgttgg
     5641 cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca tcggaaaatc
     5701 ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg ttcagtcttt
     5761 tcaaaattgt ttgcgggaaa ataatatcag tccagaagat gttgtgtttg ttgcacattc
     5821 taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg gcgtaatcgg
     5881 gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat tgaacgatat
     5941 cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg tcaaacactt
     6001 aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag cggaggtctt
     6061 ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg tttatgaagc
     6121 agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat tatatggatt
     6181 gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa tgctagatac
     6241 ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt tgatgattat
     6301 gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg ttttaaccat
     6361 gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag cgtctaatgg
     6421 tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa atggacgtcc
     6481 agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat tagatgttcg
     6541 ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa tcatcgatgt
     6601 cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg agacagaaaa
     6661 aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg ctgattacgt
     6721 caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg cagccaatgt
     6781 ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg cacgaaaagc
     6841 catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag aacagatcat
     6901 ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca agtatcactt
     6961 ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat tgattactta
     7021 ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag tgatttcttc
     7081 tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc cttcgccaag
     7141 taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg aatcaggcgc
     7201 tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa aagtaacggc
     7261 tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga tcacaacaga
     7321 agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag tttgcttatt
     7381 ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa atgctggaag
     7441 tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg cttcatgtat
     7501 gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag atatggcagt
     7561 ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag caagagtctc
     7621 tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg aagtatctac
     7681 tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa cataagttag
     7741 aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc taccagtttc
     7801 ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt atacaacaat
     7861 caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct tttgccgatg
     7921 taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca gagcaaataa
     7981 acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg atgacggagc
     8041 cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca gcgcaagctt
     8101 tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga atttatacgc
     8161 aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca aacattcgtg
     8221 cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc gatttttcgc
     8281 cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc caagggatct
     8341 tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat tacaaataaa
     8401 gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaac
//
LOCUS       DACEPW010000109         8387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEPW010000109 DACEPW010000000
VERSION     DACEPW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.110"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..386)
                     /locus_tag="IW819_001917"
     CDS             complement(<1..386)
                     /locus_tag="IW819_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1245019.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTG"
     gene            complement(379..1278)
                     /locus_tag="IW819_001918"
     CDS             complement(379..1278)
                     /locus_tag="IW819_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245020.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(1423..3201)
                     /gene="uvrC"
                     /locus_tag="IW819_001919"
     CDS             complement(1423..3201)
                     /gene="uvrC"
                     /locus_tag="IW819_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1245021.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(3383..3697)
                     /gene="trxA"
                     /locus_tag="IW819_001920"
     CDS             complement(3383..3697)
                     /gene="trxA"
                     /locus_tag="IW819_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1245022.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(3784..6144)
                     /locus_tag="IW819_001921"
     CDS             complement(3784..6144)
                     /locus_tag="IW819_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1245023.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(6259..6807)
                     /locus_tag="IW819_001922"
     CDS             complement(6259..6807)
                     /locus_tag="IW819_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1245024.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(6865..7302)
                     /gene="zapA"
                     /locus_tag="IW819_001923"
     CDS             complement(6865..7302)
                     /gene="zapA"
                     /locus_tag="IW819_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1245025.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            7504..>8387
                     /locus_tag="IW819_001924"
     CDS             7504..>8387
                     /locus_tag="IW819_001924"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1245026.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFA"
BASE COUNT         2335 a         1826 c         1406 g         2820 t
ORIGIN      
        1 ccagtcagtt gttgttgtat cagtagttgt gtactttgtc ctaatgaatt ttcgctataa
       61 agcatcccat ttatcttatc attctcagtt gagactacta aatacgcatc gattttttct
      121 tccgaaagct ctttttgtgc tttttcttca gaatcaactg ctttgaactc aaaatcatct
      181 gttttgactt ggctcatttg ctgactgatt tgctcattat cagccaatac gccgatttta
      241 ttgacatcac tattttgttg agcaaagttt cctgctacat aaatgatacc cagcatgata
      301 aaagggacta atatcatgat caagaaagaa atcgacttaa cattttttaa atagacatct
      361 ttggtaataa tccaaaactt acgcatcgat ttcacctgct ttcaatttga aaatctcttc
      421 caaagttggc ggttgctggt taaacatagg aatgtagccg aactgagtcg cacgagtaaa
      481 gatttctttc ccagcttcag gatcttctaa ggtaatctct aacgtattgt cttcatgaag
      541 gatcactttt ttcacgcctt cgatttcttc tacttcctgt tggcttaaac cagattccaa
      601 gaataacttc gtacggccaa atgattcacg aatctcatgt acttttccat tcaaaaccat
      661 ttttccgttt cgcaacatga tcaaatgatc gcagattttt tctacattat ccatattgtg
      721 gctagaaaaa atgacacatg aaccatgttt tttcaattcc aatattccat cttttagcaa
      781 ttcagcattt accggatcta acccgctgaa tggctcatcc aaaatgatca atttaggttc
      841 atggattaac gtggcgatca gctggacttt ttgctgattt cctttagaca aagattttac
      901 tttatccgtc tttttccctt ttacctgaaa tttttccatc caaaagtcga tttttggttc
      961 aatctctttt cttgatttcc ctctaagttc tgcaaaataa agcagttggt cttgaatcgt
     1021 tactttcgga taaagtcccc gttcctccgg aagataaccg atgacatcat aatctttctc
     1081 cccaagatga tggccattcc ataacacttc tccttggtct tgtgtcaaaa aatccaagat
     1141 caaacgaaaa gtcgttgtct tcccagcccc gttttggcca attagcccca tgatttttcc
     1201 atcggggatt tggaaagaga catgatcgac agctgtgtag ttcccaaaag ttttaacaag
     1261 atccttgact tctaacattt cttcactcct tcttcaagaa atcacatata tccttagaca
     1321 ccagacgtaa gcgatttatt atttaccaac aaaacgtaaa cgctcttttt gttgtacata
     1381 aaatgatagg acactttact ttctttgtaa agtgcccttt cctcattcct gatgtaattt
     1441 atcaaatacg ttttgtgcga catttttcgg cagtccgatt tcttgtaatt cttctgtcgt
     1501 ggctgctgtg atatttttca acgatttgaa ctctttaagc agcatttttt ttcgttttgg
     1561 tcctaaccct tcaatttcat ctaatcggga agcaaaactg tttttactgc gtagctgccg
     1621 atggaaagtg attgcgaatc gatggacttc atcttggatt cgctggagta agaaaaactc
     1681 tggtgaattt cgctcaagcg gtactacagc caaatctgta ccgaatagca actcatttgt
     1741 tttatgttta tcgtttttta ccatgccagc aataggaata tcgattccta attgattttc
     1801 taagacatct tttgccacat ctacttgtcc ttttcctcca tcgatcaaga tcaaatcagg
     1861 taaaggcaac ccttcgcgaa tcactcgcga atatctgcga tagatgactt ctctcattga
     1921 tgcataatca tccggtccgt tgactgtttt tattttatat ttccgatatt cttttcttgc
     1981 cggttttcca tcaatgaata cgaccatcgc agccacagga ttcgtcccca tgatattaga
     2041 gttatcaaaa gactcgatac gtattggggt cgggatattc atcgcgtttc ctaaacgttc
     2101 aactgctccg atcgtccgtt cctgttttct ggcaatcaga tcaaaccgtt cattcaaagc
     2161 aactgaggca ttcttactcg ccaattcaac taattttttc ttttctcctc gttttggttg
     2221 cagcactttc gtagctaaca aagcttctac actgggcttg tcgatcgtat caggaatcaa
     2281 gacttcttta ggaatgaaat gctcattttc ctgataaaac tgaccgaggt aagtcaagaa
     2341 atcttcttct gcttcgttat agaaaggaaa cattgaaaca tctcgttcaa ttagctttcc
     2401 ttgtcttacg aaaaacactt ggacacacat ccatccttta tcaatcgcat aaccaaatac
     2461 atctcgatcc agtaaatcag tatttgtcat tttttggcga gtcatgattg tctcaatggc
     2521 acggatctgg tctcggtatt ctgcagcttt ttcaaactcc atgttttcag cagctttttc
     2581 cattttctga tggatctctg actcgatttt ttcgtgtcct ccgtttaaaa agtttttcac
     2641 ttcatcaacg attttcgtat agactttcgg atctacgtcg aaataataag gacacaggca
     2701 ttgtcccaaa tgatagtaaa gacaaggctc cttcgtctga ctaggtttgc attttctcag
     2761 aggaaataat cgatcgagga tcttctttgt ctcattcgcc gcaccgacat cgggatatgg
     2821 tccaaaatac agtgctttgt cttttagtac cttacgagta atcaccaaac ggggatattt
     2881 ttcattcgtt atcttaagga aaggataggt tttatcatcc tttaacataa tgttgtattt
     2941 aggatcattt ttctttatca gattgatctc taagagcaat gcttcgatat tcgattctgt
     3001 aacgatgtat tcaaagtctt cgatttcact aacaagccgt tccgttttcg tattatggct
     3061 accagtaaaa tatgagcgga cacgattttt taatattttg gctttgccga cataaatgat
     3121 cgttccattt ttatctttca tcaagtaaca gccgggctga tcaggaagta aagctaattt
     3181 gtttttgatt cgttcgttca tctggtactc cccttcatat tcaattaaag ttattatagc
     3241 atgtctgtca aaaaaacaat ccagcctgac aacgtaatta tactcaaaaa atattaattt
     3301 gaataacctt aactcaaccg caaaaaaacc gaggacggaa ctggattcag tcccaccctc
     3361 agcttatttt tgctgtaagt ttttataaat attgatcgat catttgacgt agttgttctt
     3421 ttgaatgaac accaacagct ttttctacta cttgaccatc ttttttcaac attaatgttg
     3481 gaatgctcat gataccgaat tgttgaggag tttctggatt ttcatccaca tccattttag
     3541 caattctgaa ttcttcttca tcatattctt gttccaactg atccaaaatc ggtgcttgca
     3601 tacgacatgg accacaccaa gttgcccaaa aatcaatcaa tacaagtcct ttatctgttt
     3661 cttcattaaa tgtcttatct gtaattactt gtgacatatc aaagacctcc atttttaaaa
     3721 tctatgacta gtatagcatt tctatacttg gatagcgaac catctgctta cctttagtaa
     3781 gctttactta aatttaacaa ttgtcgcacc atttccgcct tggtttgccg gtgcaaactc
     3841 aaagcttttg acgctccgat ggtttttaag ataatcagta attcccttgc gaagtgcgcc
     3901 tgttcctttt ccgtggacaa tagtcacttg aggataacca gctagaatgg cagaatccaa
     3961 atattgatct acctcactta gcgcttcttc gtatcgtttt cctcgaaggt ccaattgatt
     4021 cggcacgtga ctactagatt cagatcgtac agcatggata actcgttgtg tcggctcttt
     4081 ttgaggagca acaggcgtca gctcgtcttc ggacacattc atttttaata taccgatttc
     4141 tacttgccac tgattcccgt ttttacgtaa aagcgtccct ctttggccgt aagtcgtaac
     4201 caacacttcg tctcccggtt tcaacgtttt ttgttctttc gccttcttca atactttatt
     4261 tttcttcaaa gtttcttctt gatgtagatt tgctaattgg gtctttgcat cgatcaactg
     4321 atgctctttc acatttcctt gcccaatctg ctgttgcatt ttacgaatat cagcaatgat
     4381 tttttctgct ttttcttccg cctctgacac gacttcattt gcttttttct ttgctttttc
     4441 catttctttt tcacgttctt caaagaaata gctgtaagct tctttcaaat catcgtgaag
     4501 ttcttgtgcc tcagaaacaa aatgacgcat ttccagatat tcggtctctg ccattttccg
     4561 gcgattctct aaatctgtga tcatttcgtt taaatcttgg ctctcatcat tcatcaactg
     4621 cttcgcttca tcaataactt ctgtatcaag acctaatcgg ctagagattt caaacgcatt
     4681 gcttcgtcca ggaacgccaa tcagcaaacg atacgttgga cttaatgtat ccacatcaaa
     4741 ctccatgctg gcattaatcg tatttgcccg attatatcca taaactttca attcaggata
     4801 atgcgttgtt gccatcacat aagcagactt tttaccaaga tcatctaaga ttgcgattgc
     4861 tagagcagca ccttcttgcg gatctgttcc tgcaccgagc tcgtcaaata ataccagact
     4921 tttttcattg acttttgata aaatatctac agtgttcgtc atatgagaag aaaaggtcga
     4981 tagactttgc tcaatcgact gttcgtctcc tatatccgca aatacttcct caaaaatacc
     5041 gatttgactt tcttcacctg caggaatcgg taatcctgct tgccccatca gttgtaacag
     5101 acctaaagtc ttcaacgtga tcgttttccc acctgtgtta ggtcctgtaa tgacgatggc
     5161 ttgatagtct tttccgattg tgatgtcatt agaaactact ttttcctgat cgatcagtgg
     5221 atgtcttgct tgtttcaaga cgacatgatt gtcttcactg attcccggca cgatggcttt
     5281 tacttcttta ccaaatcgtg cttttgcatt catcagatcc attttaccaa tcacataagt
     5341 attgtggatg atttcttgac gatggggaac aagttcagca gaaagttcac ttaaaatacg
     5401 ttgaatctca tttcgttcag cgatttggta ttgacgcaaa cgattattca aatccaccac
     5461 ttgttttggt tcaataaata aagtctgacc tgaagcactt tgatcgtgga ctacaccgcc
     5521 aaatacaccg cggtattctt gtttgactgg gataacgtag cggtcatttc gcatggtgat
     5581 gatcgcatca cttaaatact ttgcattttt cccacggacg atcccatcta actgttcacg
     5641 aacagcttgt tcacttcgcc gaatgttctg tcttatgctc tttaactcag gagaagcgtc
     5701 atcagtcacg cgtccgtctt catcgattgc ttcctttaaa cggcggctca aaacagggat
     5761 cgtgactaac tgatctgccc acatatacaa acgtgcaaat tcgatttcac tgtcagaaag
     5821 atcatcaatg aaacgattca attcgcttgt cgtagacaat actctggaaa cttgtgcaag
     5881 ctccactcca ttcaagtctg ctccgatctc aatccgtttc atatggggac ggatattttc
     5941 aattttaggt atagggatcc cgcctcgcag acgttgcact ttcaatccat cctcagtttc
     6001 ttgaagccag tttgtgatcg tttgtttctc actcactggt accaattctg ccagttcttc
     6061 ttttccttgt gcagtcacta caaattgtct gaccatctgt ttgacttttt caaattccaa
     6121 tgtttcaagg attcggttat tcattttatc accttatctt tcactatgaa aaaagagctg
     6181 tgccggacat acgctacgca gccttcttaa ataaactact ctgtacaaaa aacgaaatat
     6241 tttccagcac tattttcctt aattcaaaat attcgttacc caaagctcat acatcttgtt
     6301 ggtaagaaat ggcgttctct caacgataaa acgagcgaaa ctatgctcgc cgaattgatt
     6361 ttggaccaca tctaatggaa caaaggcaag taactgcaaa atcaaaaata tagtaacgta
     6421 agccatgatt agtgacagga tgcctcctgc aatccagtct acctgtctca agatcggcac
     6481 atacgtcaat ccatggacaa aaacccccac aaatcgggta agcagccagc caattagcaa
     6541 aataatcaga aaagcagttc ctgcataaaa agcttcatct aggtgaaagg caaatacctg
     6601 atcaaaaaat gctaactttg atgttggggt gacagctggg tatggaatgt ataattccaa
     6661 atgacttgct aatggtttgt aaaaatgctg ggcagcaata aatgacaata catatccaat
     6721 cgcgaatatg atttgcaaag caaacccgcg acgtgctcca gaaaaaaacg caattagcaa
     6781 taaaaataaa atcagcaaac tcagcatgct acgttccttt ctcttctaac ttttttaaat
     6841 ttttcttatt ttgataaaag gatcttaatt agaagatttt tgtttaattt gtccttttcg
     6901 tttctcgttt aaaatctgtt gtgcttccac atgattttta atggtatgat ctgcttgacc
     6961 attttctttc aatacttccc gagcttcgtt ctcgattgct tcaatgcgtt tcacacgatt
     7021 ttcaagctct gtaaatttga tcattttttt ctttaattct gctgtttctt cttccagctc
     7081 caatattctt tcttgtttct tcaattgatc cgaaagcgca ttgacagcca tcaagattgc
     7141 tgcttgttca ttatccattt gtggtgataa ttgtttcaat tctgctaact gttcattgat
     7201 cagcttcgtt acgatatcca tatgatgctt ggtttcccgt ccgataatag tatatgtttg
     7261 gttggcaatc accgctttgt agcgtgtttt ttcgtgcgcc atacgaatca tttcctccta
     7321 tttgatgaaa gttttattta gtgtaccatg aagttggttc tttcaccgtt ctccacaaat
     7381 atttttcaca tacttaacta ttatatgata aactttaaaa aaatgctatg tctcgcaatc
     7441 tcttagaaaa cgaaaagtcg tgagttgcgg tatagtttta tgtataaaaa cgaggagaat
     7501 tttatgagca acgtcattat caaagcaaca aacgaccaac ttagtcaaat gaaagcatac
     7561 tatcgctctt atttgttaaa taaacagatt ccttatacta gctttgctgc aaaaaaagac
     7621 gggacgacca ttaccgctta tacatccgga aaagtgatgt ttcaaggtac gaatgcagca
     7681 atagaagctg ataaatgggg ggaggcacag gataaacaag ccaagccagc aaaaacagcc
     7741 tcttctttgc caaaaaatat cagtcaatta tccgttttag gtagtgatga ggtcggaaat
     7801 ggcagctatt tcggtccgct aacagtatgt gctgcttatg tgaaacgtga tcagcttgca
     7861 gaattacgta aacttggtgt tagagattca aaagagttaa aagatagcca aatcatccag
     7921 cttgctgaca tcttaaaaaa aaccatcccc tataaactgc ttgtcttatc tccagaaaaa
     7981 tacaatgaga tccaaccaaa atacaatgcc gttcggatga aagtcgcttt gcacaatcaa
     8041 gcgatccatt tgctgctaaa aaaaatcgag ccagaaaaac cagaagcgat tttgatcgat
     8101 cagtttacgc cggaagcaaa ttttaaaaaa tatgcgcgtt tagaaaaaaa tcaattacaa
     8161 gaaaaactgt acttcgttac taaaggagaa caatatcatt tagctgttgc tgctgcttct
     8221 atcatcagtc gtgccagctt tttggaagag ttagacaaag catcagctga agctggaatc
     8281 acccttccgt caggagctgg aacgaaatct gaccaagtag cggcaaaact attggagaaa
     8341 ggcggcatgc ctatgcttga aaaatatgcc aaacttcatt ttgccaa
//
LOCUS       DACEPW010000110         8386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEPW010000110 DACEPW010000000
VERSION     DACEPW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.111"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..487
                     /locus_tag="IW819_001925"
     CDS             <1..487
                     /locus_tag="IW819_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1245027.1"
                     /translation="EIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIA
                     VKVLSRSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDF
                     FMMNLIKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNG
                     G"
     gene            491..1129
                     /locus_tag="IW819_001926"
     CDS             491..1129
                     /locus_tag="IW819_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245028.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            1506..2417
                     /locus_tag="IW819_001927"
     CDS             1506..2417
                     /locus_tag="IW819_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1245029.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            2634..3071
                     /locus_tag="IW819_001928"
     CDS             2634..3071
                     /locus_tag="IW819_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245030.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            3166..4050
                     /locus_tag="IW819_001929"
     CDS             3166..4050
                     /locus_tag="IW819_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1245031.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            4272..6845
                     /locus_tag="IW819_001930"
     CDS             4272..6845
                     /locus_tag="IW819_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245032.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     K"
     gene            complement(6918..>8386)
                     /locus_tag="IW819_001931"
     CDS             complement(6918..>8386)
                     /locus_tag="IW819_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1245033.1"
                     /translation="LTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNLFSDEEIS
                     VSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGR
                     TKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVA
                     ETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQ
                     EEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFT
                     GYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVL
                     EKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEP
                     IFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPV
                     KYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT         2774 a         1421 c         1724 g         2467 t
ORIGIN      
        1 agaaatctat ccaccgttaa agatcaagat aggctctttg tcgaaagaaa ggttatttga
       61 gctgattgaa gaaagtcagt tacgtgataa cttgcagtta tcatttccgt atactcagac
      121 tgaagagata gctgtcaaag tgttaagcag atcatttcat tacttgatac agatattttt
      181 actcactttc ttttctttct tcttgttgag ctatgtcatg ttatgcatat attttgtcta
      241 caattgtcgg aaaatcgcta tctttcgttc aagtggttat tcgctctttg aaacgtataa
      301 agatttcttc atgatgaacc tcatcaaatg gggaacgaca agtgtaatct tcctttttct
      361 tatagaaaga gagcctaaat atctttttaa tatttttttc tttagcttca ttgattttat
      421 tctttcagtt gtttttattt tgtctacaga aaagaaaagt cagttattgc tgatgaatgg
      481 aggttaacaa ttggagccgt tgatagtgat aaaaggtgcg aaaaaaatca ttcgaaacaa
      541 aacgattctt catttggatc gtttcactgc ggatgctggg gattttgtga ctgtagttgg
      601 agagagcgga gcagggaaaa gtactttatt gaatatatta ggattgaatg atcaattcag
      661 ttcagggcaa ttttatttat ttaatcaaga aacaaaacat ttaactggaa gagagaagcg
      721 cttgataaaa agaaatgccc tctcttttct ttttcaagat tttgggttga ttgaatatga
      781 aacaatcgat tttaatttag atattggatt gaagttttcg aatttaaatg tttcagataa
      841 gcgaacagca aagtatgagg cattgaaaaa agtaaatata aacaagaagc taagtacgcc
      901 tatctacaca ttatctggtg gtgagaagca aagaatcgcc tgtgcgagag tcttattgaa
      961 gaatagcaaa atcattttag ccgatgaacc aactggatca ttagataaac gaaataagga
     1021 gcaagtcgtt gaaatcttat cgcaacaggc ttcccaaggt acgttagtta tcgtggtcac
     1081 gcatgatcca tatctgacca aaataggaaa caagaagatc atgctttaga aaacaaatga
     1141 aaaaacaaac tttttctaat aaaaacaaca aaaaaatgtc tggaaaatcg aacataaaag
     1201 tgtctgattt gaatatccca acagcggaag aagaaaaaca aaatcgataa catttgttct
     1261 aagaattagt tacgttaaat acggaacgca agatagactt gaaaaacggt aacaaaagaa
     1321 gtagtatatt aaggtgtgat gtgctacttg acatttattc tgctgagcga actttctgga
     1381 ttgtgtagcc ttatatctat tcaggacgta tcgagcagat tagtaaaggt gttcttgatg
     1441 attttcatct tgaacacctt tgtttttagc aattcgcaaa aaacgaaaag taggagaatt
     1501 gataaatgaa aaaaagattt ttagcacttg ccatcgtact aggcactggc ttattgtcag
     1561 gctgtacgaa tgccggtgaa aaaacagccg ttagttataa aggcggtaca atcagcgaac
     1621 aagaagtaat ggatagcttg aaaaaaatgc aaggagcaga ttccgctgtg caacagttga
     1681 tcgtttatca agtatttgaa gataaatatg gagatgacgt atcaaccaaa gaaatcgatt
     1741 cccaatatga tcaaacaaag aaacaattgg gtgattcatt tgattcacaa ttgaaatctg
     1801 caggttacac agaacaaaca ttcaaagaca gtatcaaaca aagtctagcc tttcaagaag
     1861 gtctaaagaa acatatcaaa ttgaccgatg aagatttaaa aacggcttgg gaatctttcc
     1921 atccagaagt ggaagctcaa attatccaag tagcaagtga agatgatgca aaagatgtga
     1981 agaaagctgc tgataaggga gacgatttct ctaaattagc caaagacaag tcaactgata
     2041 ctacgacaaa agaagatggc ggaaaagtga aatttgattc cactactaca acggttccgg
     2101 cagaagtcaa agaagcagcc tttaagttga aagatggtca agtttctgat gtgatcactt
     2161 ctaccaatgc ttcaacttat actacggaat attatgtggt taaaatggtg aaaaaccaaa
     2221 acaaaggcaa cgacatggat aaatacaaaa aagaattgaa agaaattgca acagacacaa
     2281 aattaagtga cagtaccttc cagaataaag taattggcga agtattgaaa gacgctaatg
     2341 taaaaatcaa agacaaagac tttgaaaatg ttttgagtac attcacttca gattctagta
     2401 cagcaagtag taaataagcg gtataaataa aaaatatgaa ctatttaaat caataaaact
     2461 gttcatctaa atctttttat tagaactaaa tcaacagtag tttaattatt acttgattta
     2521 gttcttttta ttttaaaaat tttttttact tagaatccag ccaccctaga tatttttctg
     2581 tataatctta aataaagaaa acgatattaa aaaatgcacg tagaagtaga taaatggagt
     2641 ggttttgcaa atttctttta tttctagaag ttattcacgt atacggattt gtttttctca
     2701 caaataaaaa ggcaggaagt acaatggatc atttgtatat aaaatatatt catcaatttt
     2761 ccgttttggt attaggcaca ctaattatag cggggattgt tgcatttatt attcaaggtc
     2821 gtgacatatt aacagtacat caagagtttt tcagttatgc atttggtatt cctctgatcg
     2881 ctataaggtc tgataacaaa aagtggaaga aacgctatta cattctcttg ctatttttgt
     2941 gtttttatct gccacctaag atttttactt tattaggtat acaatgccta tcattttctt
     3001 ttatttccta tatcattgtt ttaattttcc tcagtttcat agtatgggta ttttatcttg
     3061 tcaaaaaata aatgaatgtt tcatgaacag tacttcatca cagcctaatt tttttcttta
     3121 tgctatcgtt atattaaaga aatcaatcag aagggaaaga aagtgatgaa aaaagtttta
     3181 ttagtggtaa accccagttc aggcggtgaa caagcaaagg aatttgagca attagcgata
     3241 gcaaaattgg aatcggtgtt tgatgaagtg gtcgtcttgc atacaaaaaa agcaggggat
     3301 gcaaaaaact ttactcgcga agcggctacg gaagggtatc atagtgtgtt tgtcatgggc
     3361 ggagatggaa cagtcaacga aggaatcagt ggaatcgctg aacaagaaca tcggccaaat
     3421 ttcggatttt tcccattggg tacggtgaat gacttggctc gagcattagg gataccgcta
     3481 gaacctgagg aagcgatcaa tcatttttcg attgaatcag tcaagccatt ggatattgga
     3541 aaaataaatg acgactattt tatgaatgtc gtcgcaattg gctctatccc tgaagcaatc
     3601 aatgatgtag atgcggagaa gaagacgaaa tttggaaaac tagcttattt catgtcagga
     3661 atcaaacagt tagcttccac acaatcctac tcgtttcacg tagaagttga tggaaagaag
     3721 gaagaaattg agagcagtat cttgctgata ggactaacta actcagtagg cggttttgaa
     3781 acattacttc caaatgcaaa agtggatgat ggtaagctcc atttggttta tttaaaagat
     3841 tcttctttga tcgatacgct taaaacattg ccagacctat taaaaggagt agatgaatct
     3901 acgaaaaatc tagcctatct tacttgtgag caattagttg tgtcattagc tgatgaggaa
     3961 gaattgacga cgaatgttga tggagatgaa ggggataaat tacctgtgac aatcaaagtg
     4021 ttaccctcac atttacaggt ttattgttaa aatggaggtt gtgaccgaag agttgtcagg
     4081 aggacatcgg ttgttcggaa taagggactg tgacaagact tttgtcacag cctttttttg
     4141 tacaaaaaag aaattttcac ctaacagaaa aatttgccat aaaaatacat attttaaata
     4201 tgaaagtaca attacaaata aattaaaacg ttaaaataaa attgtcaaaa cgtcagagag
     4261 gatggtggag aatgaatcaa tatgtaaaaa agctgattgc gtcgattttg tttgtcttcc
     4321 ctctcttgat ggtgacatcc gtttttgccg atgtagagaa aacaagcgaa tcaacgcaat
     4381 cagagaatca aagttttgac aagactactt tttcagaaga atcaattact tctgaaacta
     4441 cagaatccca aagtgaggcg cctacttcta atacaaggca agaagaaaat aagacaagtg
     4501 agacctcaga agtagctgaa caaacgtctg atagcagtga agaggtacag gttagagttg
     4561 tttcagcgga tgggtacagc gaagctgcca caacagaaga actgacgcag cttctcgcag
     4621 acgaatcagt agctaagatt cgtttaactc agcctttgac tttagacagg gaattggaga
     4681 tcaagcgaga tatcgttatc gattttggcg gacttgctca taatttcggc acacatcaca
     4741 tctatatcaa tgagacacct cagtcgatcg agtttcagaa ttttaaagga acggcagctc
     4801 atccaggtgg gttgatacca aatatcgatg gaaatgccat tatcattgct tatatgtccg
     4861 atttttgggg aaacagatat cattttacgg gagaaataaa atttaccggg acacttgact
     4921 tatatgatgg ttctaaatta ggcctgattt acgcccctcg tgcaactgtc acattagatg
     4981 gtgtttccgg tgtattggat gtccaaccag tgaaagaagg gatgaatgcc gctccgggta
     5041 aagcttattt ttgtagaagc tatcagctaa atgtaatcaa tggtagtcag ctttatggac
     5101 catatctagg aaaattctat ggttttctag attcaggtga tgagacgggc agtggaaaaa
     5161 cagcaccagg actccatatc ttgagtggtt caaaggtctc tcttgactat gatcgggcag
     5221 atggcgtcag cgatgaagga gaggctgtcg atacgctacc cgggaatgtc acatttaaag
     5281 tcagtggctc aggaagtgaa ttttcggtaa atacaaagat caatattacc aacgacaaca
     5341 atcgcggaat tatccaaatg agaggctcag gatcaagagt cgctgtgtcg aatgatggga
     5401 aaatcacaat caatacagcg actactggcg gatttcgcct gcaaggagat tctagtcaaa
     5461 tccatgtatc aagtggtgga caaatctcgg tcaaaatggc tggagatggc gaccaacctg
     5521 gaaacaatgg gatgcgtttt gtagggaaag gactttcttt gatcgtagaa ggagccggat
     5581 cagtcatcga tattgataag caaagtggtc gttctagcgc agttcgtttt gagaacggta
     5641 cgcaaaaact aacagtaact aatggaggct cattgaaggt aagaaatgca ggggatggca
     5701 aatcttatgt gaacagactc aatcaaggaa accaagcgat ccaattttat gacgcttctg
     5761 gttttctaga aagtccaggg tcagctgatt ttacggttac tggagaaaaa tcaaatatcg
     5821 atatacgcgc agattccgga gctaccgtag atacaactgg agatattgat ttgaacttta
     5881 tggctggcga aaaaacttat ttagtcatgg tcgggaaaac tggggaagat aagactggca
     5941 tcatcagtgg ggatgtgttg aatgtagact tagaatctcc tacctatttc gatttccaaa
     6001 acaaacggat cggaagcgag actaatggca taggtggctg ggtatttcaa ggaaatggcg
     6061 attcttcttt gaagatcgct cattcagaaa ttgctttatg gcgaaaggca ggagtaacgt
     6121 tcgataactt tgaaaaagat ccggattatt tttctgatat gacagactta tcgatcaatg
     6181 gaaccgactt aggtaatttt gtatcaagcc ctagtgcagg tttaacaaat gaatttgctc
     6241 agacaggtgc cggaatgaaa aattataatc gaatcagtgc caataaccag cttccgatga
     6301 ttgaatcctt aagagtacca acgaatgcag ataaaaaaat ctatgggcat gtcgttgttc
     6361 ccgaaggtgt cgaggcaaag ccaagagatg cttggacagg cgaagtagaa gtgactttac
     6421 gtattactta tgcagatacg acgaaatcac ctgtagaaat tactgctctc acacaaggac
     6481 aaacagatga aaatgaaggg acaggatata atccgtgggg agaaggcgaa caaggcggtt
     6541 tgtttgaggt tcaagtaccc aacgaagaaa gactaacaac gggggatcaa gtgcaaatag
     6601 ttgctgctaa aaaagtaaga ggcggccaag aagtgacaga actggactct gccaaaacga
     6661 cagtcgatgt gattcctcct gagcctgcta aattaagtac cacattgatc aatgcagctt
     6721 ctcgctcaat cacaggtaca ggcgaagcag gagctttggt ttcattgaaa aaaaatcaag
     6781 ctgttttgtc cgaaacaact attgatgata caggtaaatt tgttctttct atcccagaaa
     6841 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
     6901 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
     6961 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
     7021 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
     7081 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
     7141 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
     7201 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
     7261 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
     7321 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
     7381 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
     7441 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
     7501 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
     7561 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
     7621 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
     7681 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
     7741 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
     7801 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
     7861 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
     7921 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
     7981 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
     8041 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
     8101 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
     8161 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
     8221 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
     8281 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
     8341 aaataattga ccttttcatt caatagttgg atttcagttg taagat
//
LOCUS       DACEPW010000111         8357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEPW010000111 DACEPW010000000
VERSION     DACEPW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.112"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            3..935
                     /locus_tag="IW819_001932"
     CDS             3..935
                     /locus_tag="IW819_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1245034.1"
                     /translation="MGISYHFLSYFYAKKLVRAGVQRGYDWYEETGHRLMDPAAVTSV
                     EKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWVICVHDYRSTGKR
                     DMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWIQLILDEQPDASI
                     ILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVFPKKMIMRY
                     ANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQNATAGDKAL
                     LLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            1048..1326
                     /locus_tag="IW819_001933"
     CDS             1048..1326
                     /locus_tag="IW819_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1245035.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            1444..1680
                     /gene="secG"
                     /locus_tag="IW819_001934"
     CDS             1444..1680
                     /gene="secG"
                     /locus_tag="IW819_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1245036.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            1792..2538
                     /locus_tag="IW819_001935"
     CDS             1792..2538
                     /locus_tag="IW819_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1245037.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2541..4898
                     /gene="rnr"
                     /locus_tag="IW819_001936"
     CDS             2541..4898
                     /gene="rnr"
                     /locus_tag="IW819_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1245038.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            4920..5384
                     /gene="smpB"
                     /locus_tag="IW819_001937"
     CDS             4920..5384
                     /gene="smpB"
                     /locus_tag="IW819_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1245039.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5686..6405
                     /gene="atpB"
                     /locus_tag="IW819_001938"
     CDS             5686..6405
                     /gene="atpB"
                     /locus_tag="IW819_001938"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1245040.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6458..6673
                     /gene="atpE"
                     /locus_tag="IW819_001939"
     CDS             6458..6673
                     /gene="atpE"
                     /locus_tag="IW819_001939"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1245041.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6764..7288
                     /gene="atpF"
                     /locus_tag="IW819_001940"
     CDS             6764..7288
                     /gene="atpF"
                     /locus_tag="IW819_001940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1245042.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            7275..7817
                     /locus_tag="IW819_001941"
     CDS             7275..7817
                     /locus_tag="IW819_001941"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1245043.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7840..>8357
                     /locus_tag="IW819_001942"
     CDS             7840..>8357
                     /locus_tag="IW819_001942"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1245044.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKT"
BASE COUNT         2818 a         1425 c         1866 g         2248 t
ORIGIN      
        1 ttataggaat cagttaccat tttctttctt atttttatgc aaagaaatta gtaagagcgg
       61 gggtacagag aggctatgac tggtatgaag aaactgggca ccgcttgatg gatcctgctg
      121 cagtcacgtc agtagaaaaa aagcgacaaa aattagaaga agagtgtgaa agcttctggg
      181 aacaaggaat accgaaaacg atcaaaagtt atgacgggtt gaaactagct ggacagatgt
      241 tcctccaacc aactcagcag aataaatggg tcatttgtgt acatgattat cgaagtacag
      301 ggaaaagaga tatgtctcat ataggtaaaa gatatgcaga gaaaggattc aacgttctta
      361 tacccgattt acgtgcgcat ggagaaagtg aaggagaaat catcggaatg ggatggctag
      421 accggctaga tttaattgca tggatccaac tcatcttaga tgaacaacca gatgcatcca
      481 ttattttgca cggcagttcg atgggcgctt ctactattat gatggcaagt ggtgaaaagc
      541 ttccatctgc agtcaaagga tttatcttag acagcggata tgtttctgtt tatgcggaat
      601 ttcggtatat gttaagtaaa atcacggtct ttcctaaaaa aatgatcatg agatatgcga
      661 atcactatgc tcagaaatac gcaggttatt ctttaaagca agcatcagct acaagacagc
      721 ttggtagtaa ccatttgcct ttgttagtca tccatggtga aagagatcat tttgttccta
      781 cagaagcagc atacacgatt caaaacgcta cggcagggga taaagcatta ctgcttgttc
      841 cagaagcaga acatttggaa gcatcgatga aagatccgaa aacctattgg acagtgattt
      901 tttcatttat tgaacaaaga ataacgatgg attgattttc tcttcctttt tgtaagcaac
      961 acgaggataa ataattgttt atagaccatg agactgttaa aataaggtag gaataaaaaa
     1021 atatcagtta tttgaggagt gcggcatatg gaaatcaaag aggaaaaaaa tcgtcttgct
     1081 ttattcaatg atgagcaaca agaaatcgga gaaatgacat ggtcggatgc ggggccagac
     1141 atcatgatca ttgaccatac atttgtcgat ccagcatatc gtggtcaaaa attagctgaa
     1201 aagctagtgt atactggagt agaactagca agacgagagg ggaaaaagat tatcccttca
     1261 tgcccttacg caaaaaaaga gtttgagaag aaaccagagt atcatgacgt cttacgccaa
     1321 tcataataat aacagctgaa agatttgcac tttcagctgt ttttccattt atatagagtt
     1381 gtcacggagt tttatctatg ataaagttaa agagacgata tcttttggga aggaggctgc
     1441 tatatgtata atttaatttt gggaatcgtg atcgttttat cgattgttat ggtcattgcg
     1501 attatgatgc agccaagcaa gcaaaatagt gcagcgagtg catttaccgg cggtgcggat
     1561 caactattcg gcaaacaaaa agctcgcggc tttgaagctg tcatgcagcg atcaactgca
     1621 gtcatgggag ctgtctggat gatcttatta tttgtcctag catttttatc ttcgaagtag
     1681 tcgaataagc ctctcgcaca acggcgggcg gcttttttta ttgcaaagag aacgcttaag
     1741 aagaagttat gaaagaaact atggtaaaat gaggcgaaag aggtgaaacg agtgaaacaa
     1801 ttaccaaaac ccctttatgc aaaacatgga aaacgagcag ttttgctgct tcatgcgtac
     1861 tcaggaagtc caaatgatgt ccggatgctc gcacggtttt tagaaaaagc agattataca
     1921 gtctatgcgc cgctatttaa aggacatgga acgatggagc catatgatat cttgcaagaa
     1981 aaagcagaaa gctggtgggc agatactaaa aaagctatcc attttttaca gtctgaacag
     2041 ttttctgata ttgccgtatt gggattatcg atgggtggca tctttgctgt acgggcacta
     2101 gaagaagaat cagtaattgg cggcggtttt ttctgttcgc ccctgtctcc tgtagaaaca
     2161 aacgtgcctg aaaattttga aaaatatgta cgtcaagtgc tgaagatagc aggcaaatca
     2221 gaaaaagaaa tcaatgaaaa agctgtgtct taccgctcgt tagctgaaca tcaattaatg
     2281 gatatacaag accaagcagc aatcgcagaa agtagactat cagatatcca acagccaata
     2341 tttttagctc aagctgggaa agatgaaatg atcgacccaa atggtgtatt tgaaacggca
     2401 cgcaaattga gccgccaacg agtgacactt caatggtatc ctgaaagcgg tcatgtgatt
     2461 acagttggca cagcaagacg cgaattagaa aaagacgtgt tggaattttt agaaaaatta
     2521 ccttggaatg aggaataaat atgacaaaag aaacaataaa agagaaaatt ctctttttca
     2581 tggaaagcag tcgcaaacgc agtttttcta tggaagaaat cgctgaaggt ttagggttcc
     2641 aaaaaagcga tgattttaaa ttactagtac aaacgattgc tcaaatggaa agagaacaga
     2701 gtatcgtttt caataaaaaa ggaaaagtca aacttccttt aaaaccgatc ttggtcgaag
     2761 ggacatttcg ggcaaatgaa cgaggatttg gatttgtaac gattgatccc gaagaagatg
     2821 atatctatat cccgaaagaa gcgactggct ttgcaatgga tggggatacc gtagcaattg
     2881 atatcatgaa gcgagcaaat acagatatgg atcgcggagc agagggaaaa gtagtggaga
     2941 ttcgagaccg agcgactact cagttagccg gagaatttgt tgcttattca gacgaagaga
     3001 tacaggaaac ggatctttat ggtgtggtca tcccgaaaga tagaaagctt aaccaattca
     3061 aagtgtatgc agcggcagag ggagttcgtc cagttgacgg aagtattgta ttgatcgaac
     3121 tgacccatta ccctgaaaaa gggtatgcaa ctagtttaga agggataatc aagcaagtga
     3181 ttggtcataa gaatgaccct ggcatggata tcctctcgat tgtcgtggct aacggtattc
     3241 ctacaaaatt tcctgatgat gtgttatctg aggcagatga agttccagac cagatcagcg
     3301 aaaaggatat tgttggacgc agagatttaa gagatcagct catcgttacg attgatggag
     3361 aagatgcaaa agatctagat gatgcagtga cggtcaaaaa attagaaaat ggtaattatt
     3421 ttcttggagt acacattgca gatgtatctt actatgtgac gcaaggcagt caactggaca
     3481 gagaagcata cgaaagagga acaagtgtct atttgaccga tcgagtgatt ccgatgatcc
     3541 cacaacgttt atccaatggt atctgttcat tgaatcccca tgttccacga ctcaccatga
     3601 gttgtgaaat ggagatcgat ccaaatggac aagttgtctc tcatgagatt tttccaagtg
     3661 tgatccaaac aacagaacgg atgacctaca cagctgtcaa tcaaatctta gaagatcaag
     3721 atgaagaaac gatgcaacgt tatgctcagc tcgtgcctat gttccaagaa atgggtgaat
     3781 tgcatcgaat ccttgaaatg atgcgaatca aacgtggagc gatttctttt gaggatcgtg
     3841 aagcgaagat ccttgttgat gctgaaggac acccaaatga tatccagcta agaagcagag
     3901 ggataggtga acgcttgatc gaatccttta tgcttgctgc caacgaaacg gtagctgaac
     3961 attataataa attaaatctt ccgtttattt atcgaatcca tgaacagcca aaagaagaaa
     4021 aaatgcagcg cttctttgat tttgcttcag ctttgggcat tctggtccga ggaacgaaag
     4081 gaacgatcac accaaaagat ttacagcatg tgatcgaagg agtagaagac aaaccagaag
     4141 ctgcagttat caatactatg ttgctaagaa gcatgcaaca agcacgctat tcagaagaca
     4201 attttgggca ttatggctta gcggcagatt actacacaca ctttacatct cctattcgac
     4261 gctatcccga tctaatcgtc catcgtctga tccgttctta cagtgaagat aaaagtgaaa
     4321 gcaatcaaag ttcgtgggct gcggaacttc ctgaaattgc agatcatagt tctaagatgg
     4381 aacgtcgtgc agtagaaaca gaacgtgaag tagattccat gaagaaagca gaattcatga
     4441 tggacaaagt gggagaagaa ttcgatggga tcatcagctc tgttgtgaaa ttcggcctgt
     4501 ttatcgaact gccgaataca gtagaaggat tgatccatat caatgaattg aaacaggact
     4561 acttccattt tgttgaaaat catttagctc ttgtaggtga aagaactggt ctgactttta
     4621 aaatcggaca aaaagtgcgc gtaaaagtag tcaaagctga tccagatgaa cgagcagtgg
     4681 attttgaact cgtttccgca gaagaggtca ctgcgttgga acgtccaaaa gcacgcaatg
     4741 gaaaatctgg aaaacgagaa ggaaaacaag caggcaaacg ctcagaaaaa aatggcaaga
     4801 agaacaaacg atatagtgat agtaagcaaa cgttcggtaa gaaaaaaggg aaaaagccat
     4861 tttacaaagg tgtcaaaaag aagaaaaaga aaaaataaaa tgagttggag gaaacagcta
     4921 tgccaaaagg cgagggaaaa ctaattgcac aaaacaagaa agctcgccat gattattcga
     4981 tcatcgacac gatggaagca gggatggtct tgcaaggaac ggagatcaag tcgatacgaa
     5041 acagccggat caatctgaaa gatggattta ttcgcgtccg caacggggaa gctttcttgc
     5101 ataatgttca tatcagtcct tatgaacaag gaaatatttt taatcatgat ccgttgcgca
     5161 cgagaaagtt attattacac aagaaacaaa tcgttcgtct tgaaaacgaa ttgaaaaaca
     5221 ctggaatcac tgttgttcct ttaaaagtct atattcgtaa cggctatgcc aaggtattga
     5281 ttggtctggc aaaagggaaa aaatcgtatg ataaacggga agatttgaaa cgaaaagata
     5341 tcgatcgaca aattgatcga acattaaaaa atttctctag ataatttcat tatccttttt
     5401 cgcatattcg gtgcttttgc ttttacttaa tgataaaatt tgttagcatt taatctgata
     5461 aaacatgatt atcacaggtt ttttcttaga tcaggagcac cgtgcatggg gaaaaattcg
     5521 cttcgttttt gactgaaata aaaaagtgtc taattttcat gaaaatgttt tgaattttct
     5581 gtttcatggt ggtatgatac cgtgtggtta gaaaccgatt ggaaaaaacc agatacgcac
     5641 atgaattgat tttatgtgaa tttcggggaa aagaggtgaa atggattgga tgaacgctcg
     5701 ctaacgtttc acattggacc aatttggttt gatggtacag tttgtataat ggtgttactg
     5761 acctgtttga ttgttttctt cttagcttac ttttttacaa gaaatcttaa gttgaaacca
     5821 acaggcaaac aaaatgcgat agaatgggtc attgatttta gtcgaggaat cgtaacagat
     5881 aatctgccta gaaaagaatt gagcaatttc catttgttgg catttacatt gtttttattt
     5941 gtttttgttg caaacaacat aggtttagta acaaaaatcg tcttacctaa cgaaacaaca
     6001 ctttggaaaa gcccgacagc agacccgttt gttactttaa ctttggcatt tataatgatc
     6061 ttacttactc acttattcgg ggtgaaaaag ttaggcttca aaggatattt cgtaaattcg
     6121 ttcttgaaac cctatagttt tatgtttccg atgaaactga ttgaagaatt tacaaactta
     6181 ttaacacttg ctttacgtct ttatgggaat atttatgcgg gggaagtttt gctaaccttg
     6241 attgcaaaaa tgatgacaag cttaggctgg ttctcattac cattagcaat tccattagag
     6301 atggtctgga tcgcgttctc cttgtttatt gggagcatcc aagcatttgt ctttgtcact
     6361 ttgtcaatgg tttatatgag ccataagatt gaagtagaag aataatactc ggttaaaaaa
     6421 caaactacta tttatctatt ttaggaggaa aacaattatg aattatatcg cagcagcaat
     6481 cgcaatcatg ggagcagcaa tcggagccgg ttatggtaac ggtcaagtta tctctaaaac
     6541 aatcgaatca atggctcgcc aacctgaaat gtctggtcaa ttccgtacaa caatgtttat
     6601 cggggtagct ctagttgaag ctgttcctat cttaggggta gttatcgcct tgattctagt
     6661 ttttgccgtt taattgaatc agtattaagg cagaaacaac atcgtttgtt actgcttttt
     6721 tacatggaaa ctagaaatac tccaaagaaa ggaaggctcg cctatgctga atcaattggc
     6781 aattgcagaa gttggcaatg cgatgttagg taatatcatc gttgtcagcg gctcattttt
     6841 gatactactg gctctcttga agcactttgc ttggggacca atcagcgata ttttgaaaaa
     6901 acgtgaagac aagatcgcca atgatttaga ttctgcagaa caatctcgca tcaactcagc
     6961 gaaaatggaa caagaacgcg aacaacaatt gttagcctct cgttctgatg cagctgatat
     7021 catcaaaaat gcgaaagaaa gtggagaatt aagccgccaa aatattttga aggatgctca
     7081 agaagaagca gctcgtctaa aaagcaaagc ccaagctgat atcactgtag aacgtgattc
     7141 agcgctgaac tctgtaaaag acgacgttgc agaactctct cttcaaatcg cggaaaaaat
     7201 cttgaacaaa gaattatctc cagaaatgca tgaatcatta atcaatcaat acattgaagg
     7261 tctaggttct tcaaatgaaa ctagataaat atacagtagg aagacgttac gggaaagctt
     7321 tatttgaact ggcgatcgat tcaaatcaag cagaagaaat ctatcaagat ctcgtaagtt
     7381 tacgtcaaat ctatgaacaa gtacctggat tagggaatat gttaagtgat gttcgtctag
     7441 aacctcatga aaaacgagaa atcatggaca agcttgtcag cggttatgat ggaatcgtta
     7501 aaaacttttt agaagttgtt tatgagtata accgaatgga tgaccttttg ttcatgattg
     7561 aagaatatga acgtcgatat gatgaacatc aagggttact attgggaagt gtcacaacag
     7621 cagtcccact ttcagacgaa caacgaatac aattagaaaa gaatgttgca aaaacaatgg
     7681 actatcaatc agttgaatta aaacaaatcg ttgattcttc tattattggt ggcgcgattg
     7741 ttgaagccaa ccatcgagta atcgacggaa gcattcgcac gcaattagaa aaaatgcgta
     7801 atcaattaaa cagatagtac aaagaggtag gtgaatcgaa tggccatcaa agcagaagaa
     7861 attagtgcct tgattaaaga acaaattgaa aattatcaaa acgtgctttc agtcgaagaa
     7921 atcggtaccg taacttatgt aggtgacggg atcgctcgtg cacatggttt agaaaacgcg
     7981 atgagtggtg agttgcttga attttcaaac ggctcatacg gaatggcgca aaacttagag
     8041 tcaaatgatg taggtatcat tatccttggc gatttcgagt ccattcgtga aggagataaa
     8101 gtaaaacgaa caggtaagat catggaagtt ccagttggag aggccttgat cggtcgggta
     8161 gtcaatccgc taggtcaacc aatcgacgga ctaggtgaaa tcgttactga caaagctcgt
     8221 ccagtagaag cgatggcacc aggtgttatg caacgtaaat ctgttaacga accaatgcaa
     8281 acagggctaa aagcgatcga cgcccttgtg ccaatcggac gcggacaacg tgaattagtc
     8341 atcggtgacc gtaaaac
//
LOCUS       DACEPW010000112         8207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEPW010000112 DACEPW010000000
VERSION     DACEPW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.113"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..1084
                     /locus_tag="IW819_001943"
     CDS             2..1084
                     /locus_tag="IW819_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1245045.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1303..2445)
                     /locus_tag="IW819_001944"
     CDS             complement(1303..2445)
                     /locus_tag="IW819_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1245046.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGICLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2450..2635)
                     /locus_tag="IW819_001945"
     CDS             complement(2450..2635)
                     /locus_tag="IW819_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1245047.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2989..3234)
                     /locus_tag="IW819_001946"
     CDS             complement(2989..3234)
                     /locus_tag="IW819_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1245048.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3228..3629)
                     /locus_tag="IW819_001947"
     CDS             complement(3228..3629)
                     /locus_tag="IW819_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1245049.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3814..5622)
                     /gene="uidA"
                     /locus_tag="IW819_001948"
     CDS             complement(3814..5622)
                     /gene="uidA"
                     /locus_tag="IW819_001948"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ1245050.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KLITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWKERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLQKRWSSIPNFSYKK"
     gene            complement(5645..7411)
                     /locus_tag="IW819_001949"
     CDS             complement(5645..7411)
                     /locus_tag="IW819_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ1245051.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IISDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIEPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7424..>8207)
                     /locus_tag="IW819_001950"
     CDS             complement(7424..>8207)
                     /locus_tag="IW819_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1245052.1"
                     /translation="IFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQDWNN
                     INLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYYQSEDL
                     LKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEEWERH
                     ELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFFYPIL
                     VNKEKESWENLRTDVLFKCLSGEYVDIEQKFIDTFWKNLTNK"
BASE COUNT         2628 a         1580 c         1104 g         2895 t
ORIGIN      
        1 tatcaatgat gatggctcaa gaaaaaaagt ggagaaagtt ggcggagaca cccgaccaga
       61 ggccgaagct gctttacgaa aagttttatc agatattgac gaaacaggac aatacttttt
      121 aggtacggat actcgagtaa aacaatacct tgatttttgg atggaggaat acgttaaact
      181 aaatctaaaa tacaatacct atgaaaacta ccgatttacc atcaaaaatc atataaacgg
      241 ttatttagga aagaaaaaac ttacggatct ctcccctgct cttttacaaa atttcatcaa
      301 tgctgaattt aaaaagggtt actcgaagaa aacaatgact attactcact ctgtccttaa
      361 gaatgcgctg aatatggcgg tttatccttg gggattaatc aagcaaaatc ctatgctgta
      421 tgtaaaaata ccaaaatatg aagcacgacc aacgactaaa aaagatctaa aaatcatttc
      481 tcttgaggac tttgatcata tgctagaaat tactcctgaa ggacatcctt tctatattcc
      541 tttgaatatt ggattttata cgggaatgcg cgttggcgaa gtttgtggtc tgacgtggga
      601 tgatgtcgat ttttcaaatg gaacaattac tgtagagaaa caaatggtaa agaatgacgg
      661 cgcatgggta tatggtacgc caaagacaag cagttccaat cgaacgattt ttattggaca
      721 aaccttgcta gcaattctga aaaaacataa gaaacaacaa ttagaaaatc gaatgaagta
      781 tggaaagctc tacattgatt caaatgcagt atgtacgaag gaagacggtg agctagttac
      841 gccaagtgta gtgaaatgga atactaggag gatatcgaat gcactctccc tctcttttaa
      901 cttccattct ctcagacata ctcatgctac acttcttctc gaaaatggcg caaaaatgaa
      961 agaaatctct gaacgattgg gccacagcag aatttcaatt acgatggata cttactcgca
     1021 tgtaacagat aagatgagaa atgaaacggt cgatatcatg gaaaatctta gaaagaattc
     1081 ttgatttttg ccaccgaaaa aaaccaccgg tggcaaaccg gtggcaaatc catgtaaaca
     1141 tggtttattt tttacactgt tttcaaacaa acccttgcta tttcttcggt tcgtcctcgt
     1201 ccattttcga taccgacagc tagcttataa cccaaggtat atcaacataa aaaaaaaaaa
     1261 aataggctgt gacagcagcg tgacagcaaa aaagatggaa agttaaatat caaacctcgt
     1321 aatttcattt gcgagtgctt gatttgtttc tggatataag tgtccatatt tgtccaaagt
     1381 agttttaata gaggcatgac ctagacgctt agatattgca tagatatttt tattatgttc
     1441 aattaaaaag gcaacatgcg agtgtcttaa tccatgcata tgaattggtt ttactcctgc
     1501 aatttcagct aattttttta ttctgcttaa aatagttctt ggagatggcg gtaatccatc
     1561 aaaagaaaaa accaattcta tatttttccc aattgattgt tgagtttttt tccatgtttt
     1621 taaatcttca acggtttgtc tgtctaacga aattatccga atacttgatg tattttttgt
     1681 ttcagcaaat tcataatctt gtcttgtacg gatgtaaatt gacttattta cgctacatct
     1741 gccatttatt aagtcaaagt ctttccattg taacgctaag agctcctcac tccttacacc
     1801 tgtaacaaaa ataaaacgca tcaaacgttt ataaaattct tcataaaaat ccccgtgata
     1861 aacactatta tatactttct taaattcatc aactgtccaa aattctactt gaggacgctg
     1921 agtttttatt ttacctatag tatctacagg attttcttca ataatttcaa gtacaattgc
     1981 ccttttaaaa actactctca acttattgaa tatctgttta atataatttg gtgataatgg
     2041 ttgctcaatg ccctttttta caacatggat tttcgataaa aactgttgaa attcctgtat
     2101 atgaattggt cgaatcttta caacctgcat ttctccaaaa aaatccaatg cacgttgtaa
     2161 aatatgatct gttttcatat atgttttttc tacagttccc aacttatacc aaggaaaaaa
     2221 atactctcga taaaaatcat gaaacgttat tccttgcaga caaattcctt gtgaatcaga
     2281 aaaactttgt tttaactctt cgagtcccct tctagcttct ccttgagttt tgaagcctct
     2341 tctggtcact tgaattcttt ttccaaagcg atcttgtcct aaagttgcag aaaaaaacca
     2401 agtgcctctt tttctgtctt tataaacatt cgcaattttc ctcattttac tattcctccc
     2461 aagaaatatg gagaacttct tctaaaattg aaataggaat tctttttatt tttgaattac
     2521 gataaaaaaa gtatcctttc tcaactaaaa gttctttaca ttccttgaag attttattag
     2581 catagtactg agaaaaacct agattaatca aatcttttcg agtagctgtt ttcattatag
     2641 caccttcctt actttttttc atttatttct atcagctgct tttgtcaaaa gcacatagga
     2701 atttcacctc tccccaagtt tacggcgagc cttacggaag tatcattatc ccaactttaa
     2761 atcatcgctt tttattgact ataaaaatta ttttagaata tttatcgctc cttctttaca
     2821 gcaagaaatc atggcgcaac taaaatatgc tccttaaagt gttaacgtac tgatagaatc
     2881 tatttaattt tcaatgaaca ataggttttc tacctaaaag aatctactaa ttccttaact
     2941 attagaagat tcttttaaac agaaaaagtg gaaaggagat ttttttaatt agttaatttt
     3001 aaattttaag atcgcttcta ttaatttaat ttctagctga cttatcatat attcatctac
     3061 ataggtaacg aattgaccta cctcgttaaa tccatttgtc aaacataact tagccatata
     3121 actatgataa tgtttgagta tcatcttgat cgaaattgga tctccttcta gagcagataa
     3181 gataatggat agtggaacta ataggtactg ccgctttttg gatttatcta ccataggtca
     3241 taccccattt tcaattgttc aactgccttc tttcgttgat accagatacc acttgtgctc
     3301 atattgagaa ttttaccgat ttcagcatca ttaaaacctg caaaataata aagtaggata
     3361 atctttcttc ttatctccga taactgatca attgctgctg ataacttttg atctgaaatt
     3421 aatatttcca ttccaaataa gaaatagaca tcttgttcat caaaatgagt ttgggaatac
     3481 ctgccttcat ttgaatctgc caatggttcc tcattatcct ttatgttagc atttcgtttt
     3541 cgaatagact tagcttcgtt tcgtaatact tttttacagt aggcatcaaa ttggtgttgt
     3601 atagatatgt cattgtaatg ttgttgcatg ataatatgct cctttttccc cctttccact
     3661 aatacaacaa tcagagagcc cctttttttc gaaaaaacta gaaaaaaaca taaaaatatt
     3721 tatcattctt taaacagaaa atctttctat aaaaaacaaa aaaacgatta ggctaggcta
     3781 atcgtttttt gaacattctt aaaaaaatcc aaattatttt ttataactga aattgggtat
     3841 tgatgaccat cgtttctgta gatagtgagc aatcatttta ggttttctat ctctagtaaa
     3901 gattcctttc ttatttcctt gcactcttaa aatcccttga ctcgttgcaa aatctgcaaa
     3961 attccacact tgttcaccaa caaaattttc acatgtatct attacttcgt gatttgcttc
     4021 gtaataagcc acttgatact cttctgtaaa cattgttggt actgtatcat gaaatccagc
     4081 tactgtatct gctccatatt ctgtaaacat tattggcgta tcaggacatc tttctttcca
     4141 tccatctagc tcttttttca acagatattt tgcatgtgcc agatcccctc ccgaaacata
     4201 ccaaccataa taacgattta aacataaaac atcgacaata tctcctacct tacaaatatc
     4261 tggcgttgat tttagataag taactattgt tacaggtctt ttctgcggat caagttcttt
     4321 agttaattca actaaaggtt caaaatattc tttagcacct tcggaatccg aatctggttc
     4381 atttgcaata gaccacatta caacacttgg gtggttcttg tcgcgttcta tcaattcctt
     4441 gatcaccgtt ttatgagaat tttctgttcg tatctctttc cacgtattat gtttttccac
     4501 attatcatct aataaagtgg ccatgaaatt taaatgtaac cctactgcag gtacttcatc
     4561 aataactaca ataccttctc gatctgctaa tcgcataatt tcttctgagt aaggataatg
     4621 tgatgttcta aaagaattag cacctatcca tttcattaaa ttaaaatctt taatatttac
     4681 agcctcattc aaaccacgtc cattaacata cgaatcctca tgcttaccaa atcctttaaa
     4741 ataaaaaggt ttattattaa tcaaaaattt gccattggta acctgaacac tgcgaatacc
     4801 aaattcttct gtataggtat cgatcacttt attatcatca attaaactaa ttttcatact
     4861 atataaataa gcatctagag gttcccaaaa aattggattc ttaagctgaa tcattccttt
     4921 ctttccttca gcttcagcta caagttgagc ctcttcgtca tatatcgcta ttaagatttt
     4981 tccggtccat tctgtatcga tattgaattc tacacttgca tccaaatcag ttattcttgt
     5041 attaatagta atatcactta tatagtcttt cggagttgta tataacttaa caggtctatg
     5101 aatacctgca taattgaaga aatcaaaatt aactaagttt tcctttttta tatccccatt
     5161 ttcctgtact gtttccttca taatccccac aggtaaagta ctatcgtcaa taatattgtt
     5221 tacagcaact gtaattcgat tattcccgtc ttttacaaac cgattaattt cagactcaaa
     5281 tggtgtaaac ccacctttgt gttgagtaat caactttcca ttcaaaaaaa cttttgcttc
     5341 atgtgttgca gatccaaaac gaagaactaa tctttcttct aataatcttt taggaagagt
     5401 cacttctttt tcataccaca cccatccaac atgatttcta atgttctctg tttcaaataa
     5461 atcattataa gaagagggaa ctgaaatcgg ataactctct ttttcattta ttggttctaa
     5521 agcaagttct tcctgtaagt catcatttgt atctaattta aatctccaga gaccgcttaa
     5581 gtcaattact tgcctcgtct ctgtcataat gggatacaac atataaacca ctcctatcta
     5641 aatattaaat aaatgtatcc tttaaaccac aataagcatc tattggactt actcccttaa
     5701 attcagaaag acccattagt ttgtccacca atatttctaa agtttcttgt tttccatcat
     5761 aagtattaat ataagtcttt acttgaggta catcagccaa atgaaacgga cactgtacag
     5821 atacaaaaat tgttggaatt tcatgaatgt aaaacggtat atccggagtt cctttacttg
     5881 ctggccagac aattcgttga actgtgcctg gattaacatt agctatatta atgatcaagt
     5941 catatttttt agttaaattt ttaattggtc tcttagcagc atatattcca gctatatttt
     6001 taattttttc ttcttgcgat aatcctttca actcttcttc aggtgaaatg tatttttcaa
     6061 cttcaaatcc ttttgctgac aaaatctgtt tcaataaatc aatggcttga ggtctatttc
     6121 cagcaactac ttcaccaaac ccacctttta ccccatgtac atctactagt aaaatccttt
     6181 tatatttttc tggttcaata ggccaaatat cttgtttatc tttaactaat gtaattgctt
     6241 ggtcagccac ctctttaaaa attgatttat tttcaattaa accaattttt tccagagctt
     6301 cttgcttagg taataaaatt tgttcttttg cttttttgtg taatcccaat ttagctttta
     6361 accctaaaat tctttttaag gcatctaata accgatcatc agagataatt cctttacgat
     6421 aaccttccat catataatta aagtcttcat ctggatcatt aaaaaataga aatagatcac
     6481 atccagctgc aatagaagtg ggcaataatt ctgatcgttt cattgcccca gtcattccta
     6541 acatatgact agcatcagta actactaatc cattaaatcc gagttttcct ctaagtaagt
     6601 cagtgattaa ttcttttgat aaagttgcag gaagtaaatc ttcatctttc agatcaggat
     6661 taaaatgttt tgaataagct ggcatatgga tatgacctgc cataatcgaa ggtaatccag
     6721 cttctattaa acctgagtat acttttccaa aagaattatc ccattcttcc gtacctaaac
     6781 tattaatact aaaagataga tgctgatctc gttcgtcaat accgtcacca gggaaatgtt
     6841 tagctgcagg caaaattcca ctctcctgaa tacctttcat ataagctaaa ctaagttcta
     6901 aaactttatc agggttttgt ccaaaagatc gactagaaat gataggattt ctccaatttc
     6961 tattaatatc tacaatagga gcaaaactcc agttacatcc aatagccgat gcttcaatcc
     7021 cagaaactct ccctaattca taagcatatt tcgcatcatt tgttgcacca atttttactt
     7081 ctacaccaac ttcagtacca tctgtacaag ctccgttacc acctgcttcc gtattagcag
     7141 caattaataa aggaattttg ctttctgttt gtaggatata gttttgatca tatacctcat
     7201 cagcagttcc tgggttatat cgtacagctc caatatggta ctgattgacc atattactca
     7261 agtaattttt atcacgacta gatcccatat taacaaacaa ttgacctatt ttttcttcca
     7321 aagtcataga atcaatcgtc tcttcaatcc aagaaatagc atcttcatct aaattatatg
     7381 gtttttttcg taagtctact tttatagtca ttttctttct cctctactta ttagtaagat
     7441 ttttccaaaa agtatctata aatttttgtt caatatctac atactctccc gataaacatt
     7501 taaataaaac atcagttctt aagttttccc atgattcttt ttctttatta accaaaatag
     7561 gataaaagaa aactttattt ttatttgcag cttctagatc accaggagaa tcaccaatca
     7621 ttaatatctt atccaaatca caaccatttt caattacact tcgaataatg tcttcttttt
     7681 ttcctttatc ttgacaacac agttcatcaa ctaatgataa taattcatgt ctttcccatt
     7741 cttcttgaac agcttcctta ttagctgagc ttacaataat tatcttccca tattttttca
     7801 gagcttttat agtatctcta gcacttaaaa agggtttatc taatccttta gcttgactaa
     7861 tctgttgatt gacttcttcc gaccatttta aagcttttaa taaatcctct gactgataat
     7921 attctatttc cctcttcaaa gatggattag acaattcttt tgttagagat acccagtgct
     7981 ttaatcgatt aatggatgtt acatcatttt tttctaatgt tttaactaaa cctaaaaatc
     8041 tgtttattcc acgagtctct gaatataaat taatattatt ccaatcctgt aggaattttt
     8101 ctctatgatg aacatcaaaa atctctgcag caattggtcc gaaaaagaga ttgtgtttat
     8161 aagtcatcgt atccataaca cagccatctg aatcaataca aaatatc
//
LOCUS       DACEPW010000113         8098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEPW010000113 DACEPW010000000
VERSION     DACEPW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.114"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            408..2501
                     /locus_tag="IW819_001951"
     CDS             408..2501
                     /locus_tag="IW819_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1245053.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
     gene            2661..3527
                     /locus_tag="IW819_001952"
     CDS             2661..3527
                     /locus_tag="IW819_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1245054.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(3549..3986)
                     /gene="ndk"
                     /locus_tag="IW819_001953"
     CDS             complement(3549..3986)
                     /gene="ndk"
                     /locus_tag="IW819_001953"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1245055.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            4645..7323
                     /gene="mgtA"
                     /locus_tag="IW819_001954"
     CDS             4645..7323
                     /gene="mgtA"
                     /locus_tag="IW819_001954"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1245056.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIHRFQELL"
     gene            7410..8081
                     /locus_tag="IW819_001955"
     CDS             7410..8081
                     /locus_tag="IW819_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1245057.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
BASE COUNT         2677 a         1388 c         1677 g         2356 t
ORIGIN      
        1 atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc gtgtatcaag
       61 aggtcttgag cttgccgttt aactctgagc cacaaggaac aatttcaaaa aacagcttct
      121 catgttttat aaatattgtg acttgtggcc gaggagttgg caggagaaca ccgtttaaag
      181 aagaagttgt gaaaaagctg tcctgcatca actaaacagg agctgcgaca agacttttat
      241 caccttactg tttcaatcgc ttatatcaca gcggttttat gctgtgaaaa gtgcacggaa
      301 taatctttag aaagaataaa aaaatcttaa gataacaact tttttaggga attttcattt
      361 ttcaggagca aaataaggaa aaatgtgaat agagagaagt gatatacgtg acacaactta
      421 aaaaattgtg ccaaaatcgt ttagcaatcg ttttgacagc tgttttcttt ttctggatga
      481 agacgattat tgcttactat gcagattttt cgttaggtgt ggaaggtacg atccagtact
      541 ttatcttgtg gatcaacccg attgcaacta ccttattgtt ttttggcctt tctttgtata
      601 ttaaaaagcc aaagccagca ttagccattc ttttgatcat tgatatttta aacacgcttt
      661 tgttgtactt gaacattatt ttttatcggg aattcacaga tttcattaca gtcaaatctg
      721 tattgggatt ttcgaaagtg tcgcaagggt tatcaggaag ctccttttct ttgatgaaac
      781 cccatgatgt cctctactgg ctggatattg ctacgttcat tgggatactg gtttggttga
      841 cgatcaagaa aatacctatc aaaagtcaac ctgtcagcaa accgatggct atcgctgtga
      901 cctgtctttc agggcttatt ttttcaggaa atctagcgct gagcgaagcc aaccgtccac
      961 aattgctgca gcgaacattt gaccgttcat atattgtcaa atatctagga atcgatgcgt
     1021 atactattta tgatggaatc aaaacaggaa tgactagcag tgttcgcgcg catgcaagca
     1081 gtaatgggat tgatgaagtt ttagattata caaagaaaca ttatgcagag ccaaatcccg
     1141 aaacatttgg gatcgctaaa gggaaaaatg tgatcgttct ccatttagaa agtttccagc
     1201 agtttttgat taatatgaag gtagacggac aagaagtcac accgttttta aatagtattt
     1261 tccagaatca agcaacgatc agctttgata atttttttca tgaagtagga cagggaaaga
     1321 caagtgatgc tgaaaatatg ttggaaactg gcacatttgg tttacctcaa ggctcattat
     1381 tcacagaact tgggtcagat aatgtctttc aagccgcacc agcgatcctt ggacaaaagc
     1441 aaggctatac aagcgctgtt ttccatggga atgtagctag tttttggaat agagatcatg
     1501 tatataaaaa cttaggttat gacaatttct ttgatcgatc ttattttgat gaatcagatg
     1561 aaacgctagg ttatggaata ttagataaag atttattcag tgaatctgcc caatatcttg
     1621 aacatctaca acagccgttc tacacgaaat tcctttccgt aaccaatcac acgccttatt
     1681 atacggatga taagaatttc gattttccat ctttgaacac tggaaatagc acagtagata
     1741 actatgttcg aacggcacat tatttggatc agtcattgga acaatttttt acgtacctga
     1801 aaaaatccgc tatttaccaa aattcgatct ttgtgattta tggggatcat tttggcatct
     1861 caaatacaga taataaagac ttagcaagtg ctcttggaaa agacccagat acatggaatg
     1921 aatttgacaa cgcacaaatg cagcgtgtgc cattaatgat tcatatacct ggttatacta
     1981 aagggtcagt caatcacgaa tacggtggag aaatcgatgt tttgccgaca ttacttcatc
     2041 tcttgggaat cgatgataaa gactatctcc attttggtac ggatttatta tctcctcagc
     2101 atgatcaagt agtggctttt cgaaatggga acttcgtgac acccaaatac accatcttag
     2161 gaggaaaagc ttacaataac caaacaggag aaatcatcga tacgaaagca gcaggaatcg
     2221 acgaggaaat ttccaaagat caggaaaaag tcaaatctgc tttaagtctt tcagataagc
     2281 tgaatcaaga aaatctcttg cgtttttatg tgccaaacgg atttgaaacg atcgatccga
     2341 aaaaatatga ctataaaaat gaagcagaac gtaacatagc catcgaaaag caaaaaggcg
     2401 aacattcgac aagtgtcttt tcaaaaaatg gcaataaaac aacaaccaac ttatatccag
     2461 taacaagaac agacgttgaa cagcaaaaag ataatcaata aacaatcaaa ccaaccttca
     2521 gtagttagac aacattctaa tgcttgaagg ttggtatttt ttatgaaaaa aacatttttt
     2581 tatttttttc gtgtattttt cctttttaac gtatctattt attgaatgtg gaaaaattta
     2641 aattaaaaag gagaactatt atgtatgatg gtgaattgat caagaaactt cgagtcaaca
     2701 agaagttgac acagtcccaa ttagcggaag gaatctgttc taagacttct ctagtcggga
     2761 ttgagacaaa ttcagtgaaa aaaatgtcat ttgtaacatt gagatcattt ttagagcgaa
     2821 tgaacatgac acttgcagaa tatgaatgga tgcgaaacca gctggatgaa ccaataaagt
     2881 taaagaaaaa aagacgaatg ctagatcggg tacaagaaga cagctttgat ccttataaag
     2941 agatcagcaa taaccgtagg cattttaaga aaacagaaga tctgtattat ttggttttga
     3001 gtcttcacat ggtcgaaaaa acagaagtat ctgagacaga atttgaattt ttacgttatg
     3061 aatgccgaat cattgaagaa tacttgatgc agataccaga ctatggtcaa tttgaactat
     3121 ctattctagc tgattttcct tggatattca gtgatcggtt cattgagaac aattatttga
     3181 aaatcaaaaa aagaatgcga cgattagcac agttgaacaa tcatgagcag tatctcttta
     3241 cttttttaat gaacctgact tcgttttata tagaaaatgg tcggttgaaa aaggcacgta
     3301 gtgtgaatga agatatgaaa cggagcttag cacataaaga aagaagtaca gtgatctatg
     3361 aaacattgat ggcagattat tatcaaagat tgatttctgc tgctttaggg gaaaaaaaga
     3421 cgaaagacta ccaagaattc ttcacagtgc tcgaatatat gttaggaaag aatcaacgga
     3481 ggtccttaga gaaaaaactg ctggccattg ctgaacccag caactagacc agcagtttag
     3541 aatttgtatc acttcgccgc tttttcagac ggtttgttta tatcgaatgg cgggaaaaag
     3601 cgggcgatct cttttaccgc agcatcacgg gaatcagaag cgtgaatgat gttttcagtc
     3661 cccggcagcg cgtagtctcc tcggatcgta cctggaacag catcgacagc ttttgttgcc
     3721 ccaaccatct tacggacgat atcgatcact tcctcgccga ccagcacaag ataaacgaca
     3781 ggaccggaag tcatgtaatc gatcagttca tcaaaaaaac tgcgctcagt caagtgctga
     3841 taatgttctt ttgctaattc aggagtcata gtttcaaact tcatttcagc aatggccagt
     3901 ccctttgctt caaaacgttg aatgatgctt ccgactaaat ggcgtctcac gccgtctggc
     3961 ttgataataa caagtgttct ttccataaca gcacctcgtt tcctactata tttctattat
     4021 agcgggaggt ggaaaatgag acaaataaaa ccgcgtgaga caggaatata acgtgtgtat
     4081 tttaaaaaaa gtatgaaaaa catggaaaat tgatttgaca tcctcttttt tagttgttat
     4141 attaacgaag gaaaaccgaa taagatttgt taggtgaggc tcctatacaa acataggcta
     4201 ctgcccaaaa acgtcgagag acgccaatgg gtagaacagg aattgtcgag ataaggcttt
     4261 tcataaggta gctaaaagca ggtatctgct ttttacgttg tacagtgtca aaactcaacg
     4321 atgagatctg agaagctttt tttaatatgc tttttcaaac ctttcgctga gtatttggcg
     4381 agaggttttt ttgttgccgc aaatccccat cggtgaaaaa taaaaaggag gtggtctaat
     4441 tgggaaaata actattcgaa aaatgattgg aagtcatgaa ttaaaattac ttagtaaagg
     4501 agatgagaac aaatggatga cagtcattcg aaaaacaagg atctagagga aaggccaagg
     4561 ctattatcta tctgttctca gcccctttca gttttttctc tatcgatccg atgaatagct
     4621 aaagaccaaa agcagcgaga acagctgtct tttggacaca aaggagagat tgagatggaa
     4681 aaaaagaaag atacacaaaa aatctatgca gcttttgctg gaaaaacgaa acaagaatta
     4741 tttgaactat tccacgcatc agcaaatgga ttgacagaag aacaaatcga acgttcaaga
     4801 gaaaattacg gtgaaaatgc gatttcatat ggcaaaaaaa caccattcat cgtagaaata
     4861 ttgaaagcat acatcacgcc atttacatta gtgttgatcg cattaggaat tatttcattc
     4921 atcacggaat atgtattagc agctcctgga gataaagatc ttttcggtgt agccattatc
     4981 ttcactatgg tcatcgtcag cgggacaatg acgttgattc agtccgtacg ttccaatcaa
     5041 gcggctgaaa aactgaaatc acttgtgaaa gtaacggcag ctgtaaaacg taatggaaag
     5101 tacacagaga ttcctatgga agaaatcgtc tgtggagatc ttgttcggct ttcagcaggg
     5161 gatatgatac cagcggatat gcgtttattg tccgcaaagg atttattcat ttcccaagca
     5221 gcattgacag gggaaagcta tccagtagaa aagcatgcga aagtatgtga agacgatcag
     5281 caaagcgaaa caagttacga aaatcttgtc tttatgggaa gtaatgtcat aagtggaagt
     5341 gcggaaggaa tcattgtttc tgttggaaat gaaacactat ttggtcatgt agcagaaacg
     5401 ctatctgaaa aaccaattca atcaagcttt gaattaggca tccataaaac atccatgcta
     5461 ctgatcaaat ttatgtcatt gatggctcca gcagttatcg tcatcaatgg gcttacaaaa
     5521 ggggattggc tagaagcttt tctttttgga ctatctgtag cagttggtct aacacctgaa
     5581 atgcttccaa tgatcgttac gacgaatttg gtcaaaggag caagtgtgat ggccaaaaaa
     5641 ggaacagtga tcaaaaattt gaatgccatc caaaactttg gtgcaatcga tgtgttatgc
     5701 acggataaaa cggggacatt gacacaagat aaaatcattc ttgaatatca tatggattgt
     5761 gaaggaaatg aagatgatcg tgttttgcgc catgcgtatc taaatagcta ctatcaaaca
     5821 gggctgaaaa atttgttaga tgtggctatc attgatgaag caacagaaac tttagataca
     5881 gataagatca attatcaaaa agtggatgaa attccttttg attttgaacg tcgtcgtatg
     5941 agtgttgtgg ttgctgatcc agctggtaaa acacaaatga tcacaaaagg cgcgattgaa
     6001 gaaatgctga acatttctaa atttattgat cttggcggta ctgttacacc attaacaaaa
     6061 gaacgaaaag aagccgtact tgcaaaagta cagtcactta atgaagatgg cttacgagtg
     6121 attggtgtcg cacaaaagac caatccaagc gtcgttggag aattttcagt caaagatgag
     6181 tcagacatgg ttttgattgg ttatcttact ttccttgatc caccaaaaga aacaacgaaa
     6241 caagcgttga aagctctgaa agaacatggt gtagctgtaa aagttctgac tggggacaac
     6301 gaattagtta cacgttcagt gtgccgtcaa gtaggtctag agatcaatga attggttacc
     6361 ggtgagaaaa tctcggaaat gagtgatcat gaattagcac aagtagctga agaccatgaa
     6421 gtattcgtaa aattgaatcc tcagcaaaaa gcacgtttga cgacagcttt acgtaaaaat
     6481 ggtcatactg tcggattttt aggagatggt atcaatgacg ctcctgcaat gaaagtggct
     6541 gatatcggta tttctgtaga tacagctgtg gatattgcga aagaatctgc agacgtgatc
     6601 cttttagaaa aagatttgac gattttggaa agaggccttc tttctggacg agaaactttt
     6661 gggaatatca tgaaatatat caaggctaca gcaagttcta attttggcaa tatgttttcc
     6721 gtgctgattg caagtatgtt cctgccattt ttaccaatgc tgcctttaca acttctgttt
     6781 ttgaatctaa tctatgatgt ttcctgcatt tccttgccat gggatcggat ggataaagaa
     6841 tatttggacg agccaaaaaa atggaatgca tctagtattg ggaaattcat ggtttacttt
     6901 ggcccaacaa gttcgatatt tgatattact acttacttgt tgatgtattt tgttatttgt
     6961 cctgctgtag ttggcggaag tttccataca ttggatagtt cacagcaagt gattttcatc
     7021 gcattattcc atgcaggatg gtttgtcgaa tcactatgga cacaaacctt agtgctccat
     7081 gcactgcgta caccgaaagt accatttatc caaagcaacg taacatttgc catgtttgtt
     7141 atcactacat taggaattat tgtcggttct gtccttcctt ttacaagttt tggtgctgaa
     7201 ttaggcttga tgccacttcc tggcaattac tggttatggt tatttgctac agtcatcgct
     7261 tatctggcgc ttgtgacagc tgtgaagaaa atctatatcc atcgattcca agaactctta
     7321 taaacgagaa ggctggttct ttgcttctgt tatttttgga ccagtcgtgc taaactatgt
     7381 gtattgtttt ggaaaggatt aatgtataga tgaaaggtgc aatatttgat ttagatggtt
     7441 tgttagttga cacagaaaaa acgtatcgtg atggctggct ttggggattt cagcaatttg
     7501 atatccatat cccaaaagaa atcgttgacg cttggggcgg aaaaaactgg aaacaaagtt
     7561 ttgatatttt agtgaaagca gcaggtagcc ctgaaaaagt acaggaagtc cgagcgaagc
     7621 gtgaagaata tttttatcaa cagctgacaa gcggagccat ccacttaaaa ccatatgcaa
     7681 aagaaatatt ggcagactta aaagaacgtc atatgaaact aggcattgct acgaccactg
     7741 ttacgaaaag agcaacagat atcttggatc actttaaatt agctgattac tttgacacgt
     7801 ttacattcgg agacgaagtg tcggagaaca agccttcacc gattccttat ctgacagctt
     7861 tggaacgaac aggattagaa gctagttcag cctttgcagt ggaagattct ttagtaggag
     7921 caacagctgc ttcacgagca ggattaggcg ttgttttgat acctgattcc agttttgtcc
     7981 gaaattatac cgtcgaagaa aaggaaaaat tgaaacttct tgctgaagga aaagatcttc
     8041 gaactgttat tgaaatgtta gataaaaaaa cagaaaaata aatatagaaa aaataatt
//
LOCUS       DACEPW010000114         8063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEPW010000114 DACEPW010000000
VERSION     DACEPW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.115"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1031)
                     /locus_tag="IW819_001956"
     CDS             complement(<1..1031)
                     /locus_tag="IW819_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1245058.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIA"
     gene            1229..2119
                     /locus_tag="IW819_001957"
     CDS             1229..2119
                     /locus_tag="IW819_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1245059.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(2192..3016)
                     /gene="nadE"
                     /locus_tag="IW819_001958"
     CDS             complement(2192..3016)
                     /gene="nadE"
                     /locus_tag="IW819_001958"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1245060.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(3028..4500)
                     /locus_tag="IW819_001959"
     CDS             complement(3028..4500)
                     /locus_tag="IW819_001959"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1245061.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(5137..5499)
                     /locus_tag="IW819_001960"
     CDS             complement(5137..5499)
                     /locus_tag="IW819_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1245062.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            5722..6474
                     /locus_tag="IW819_001961"
     CDS             5722..6474
                     /locus_tag="IW819_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1245063.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(6614..7537)
                     /locus_tag="IW819_001962"
     CDS             complement(6614..7537)
                     /locus_tag="IW819_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1245064.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(7552..7824)
                     /locus_tag="IW819_001963"
     CDS             complement(7552..7824)
                     /locus_tag="IW819_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245065.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2479 a         1634 c         1334 g         2616 t
ORIGIN      
        1 gcaatcttta ctctatctgc atgtttcaat aaagtgatta atagacagcc taatagtaat
       61 gcatcttcaa aattatagtt atcttctagt aaaggaggag caatctgcca aggcgctgct
      121 tgctcatctt gttccgctga atgataccaa atattccatt catcaaagga caggttgatc
      181 tgcttcttac tatgtttctt cgccttgact gtatcgcaca tcgctgtcac ttcagaaatg
      241 aaccgatcca tgtctaagga acgtgcaaga taatgagaaa gatcattttt ttggttgcca
      301 taatattgat gcagagacaa ataatctatt tgatcgtacg cttgatcaag aactgttaac
      361 tcccaatcac caaatgtagg catttgccga ttcgaacttc cgcataaaac cacctcaatt
      421 gaatcatcta cgagtttcat cgccttagcc gtttcctctg ctaaccgtcc atattcttcc
      481 gctgttttat gtccaatctg ccaaggaccg tccatttcat tccctaaaca ccatgtctta
      541 attccaaaag ggctctcttg accattcgaa cgccttaaat cactccaata agttccttta
      601 ggaaaattac aatattcgac taaattacga gctgcatcta ctccacgagt acccagattg
      661 acagccatat tgatttcggc ccccacatgc tgacaccagt tataaaattc atgaatccca
      721 acttcattcg tttcaattgt ccgccaagct aaatccaacc ttctaggtct atttgctatg
      781 ggcccaatac tgtcttccca acgaaatcct gacacaaaat ttccaccagg atatctaatc
      841 atgggaatgt tcaattcttt taccaagtga atgacgtctt ggcgaaatcc acttttatta
      901 gctgatggat gatctggctg ataaatccct tcatatactg ctcgtcccat atgttcaata
      961 aatgaactgt acaaccgcgg atcaactttg cctacacgct tgacactact aatcaacatt
     1021 gtggcttcca taactgacct cctttaattt atacatgaat aatacacctc gttgaaagcg
     1081 ttgtcaataa tataaatgtt aaacttactt attttttatc gattttaatt aaccaaaata
     1141 ttaataatga ttaaaacaaa taatttaata taacttttat taacaaatat gttaatatat
     1201 aagaaaaacc ttttaagagg tgatatatat gcaactacaa ataaacgaag aatccttacc
     1261 ggtatatgaa gcattagcta gtaaaacaag gatcaagatc atccagctac tttccaaaaa
     1321 aaagatgaat gtcaaagatt tggcaaaaga acttggagtg agcagtgcta tcacgacgat
     1381 gcacgtgaaa aagctagagg aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa
     1441 aatttcgagc cttcgtgtcg ataaaatcga tattagtttt ccagaaaaaa tattcaatgc
     1501 ttttgatacg aaagaaacat cgatacctat tggtcactat acaaattatg caattgaacc
     1561 aacatgcggt ttagctacga tccatgattt tatcgggaaa gtggatgaac cacgctattt
     1621 tatggatcct cgaaggatgg atgcccgcat tctctggttt accagcggat ttgtcgagta
     1681 tcaggcacct aattttttaa atacagagga tacattggag atgttggaag tttctgtgga
     1741 aatttcttct gaatttcctt tttcaaatga caattggcct tcagatatca ctttcagcct
     1801 aaatggtgtg gagctaggaa catggacgag ccctggagat tttgctgata tccgcggtaa
     1861 atatacgcct gattggtatc cagataatct taatcaatac ggcttgctga agacgatccg
     1921 aattaccaaa cacttgacga atatgaatgg agaacccttg tcaaacatca cgatcaacga
     1981 catccctaaa gagcaagaca cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca
     2041 tgtggggggc tgtactttat ttggtaaagg attcggtaat tacgatcagg atatcaaatt
     2101 aaaagtttac tacagctgat atttttcttt tctattttat aaaacaaaaa cagccaatcc
     2161 ctatgaaggg acggctgttt ttgctttatt gttatttcca gaaatcatcc aaaacggaaa
     2221 ttggcaaatg tcgtttatgt tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt
     2281 gagcgggaac ttcttttcct tctagataat catcaatttc attatatgtg acacctagtg
     2341 cgacttcatc tgcaatcatt ggtttcccat cttcaagatc tgcagtcgga atttttgtgt
     2401 ataatttttc cggtgcattc aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac
     2461 ggaataatgg caaaatgtcg gctccaccat caccaaattt cgtgaagaat ccagtaatgt
     2521 tttctgcagc atgatctgtc cctaatacgg ctcctgctct ttctccagct acggcatatt
     2581 gcgtaatcat acgttgacgt gctttgatat ttcctttgtt aaaatcgctg attcccacac
     2641 ctgcttcgct taatacttgt acttgtgcat caacagctgg tttgatattt actcgcagac
     2701 taacatcggg ctggataaag tcaagagcag cttttgcatc ttcttcatct gcttgttcac
     2761 cataaggaag acgaacagcg ataaattgat agttttcatc ttttgtttct tcacgcattt
     2821 cttccatcgt tagttgcgcc aaacggcctg ctaaagtaga atcttggccg ccgctgatac
     2881 ctagtacaaa agttttaaga aacggatatt ttttcaaata tgctttcata aagtcaatac
     2941 ttttacgtat ttcttctttt ggatcaattg ttggtttgac acctaattca ttgataattt
     3001 ctgtctgcag gctcatttat tctcctccta tgcttctgta tgaagttctg ctacgctttt
     3061 tttcattcgt tccatgatag ccattttatg gttccagcat tcagtagaca gatcgactgg
     3121 gtatttttgc ggattcaagt ctcgtttata ctcttcccat agtgaatcta gattttcttt
     3181 tgtatattct ttgatttcgt ttagtgcagg taattcgtaa actctttttc cttcgataaa
     3241 gatatcttgt aaaactgggc gagctgtaaa gtctcgaaca gttttattga taaacgtatg
     3301 cactggatgg aacatgaaga tagcttcttc ttctcgaggg tcttcatccc ataatgtcac
     3361 gtagtctcct tcagatttcc catcaaaatt tcgagtgatg cgccacactt gttttttccc
     3421 tggagtagtt actttttctg cattgcttga taacttgatc gtatccatca tttttccttc
     3481 atcatcttcg atcgatacta gcttgaatac tgcaccaagg gctggctgat cataggctgt
     3541 gatcaatttt gtcccaacac cccatacatc aattttagct ttttgcatct ttaaattcaa
     3601 aatcgttgct tcatccaagt cattcgaagc atagattttt gcttccgtaa atccggctgc
     3661 atctaattgt tcccgaacac gcttagatat ataggccata tctccactgt cgatgcgtac
     3721 gccttggaag ttgattttgt cacctaattc tttcgccacg cgaatcgcac ttggcacacc
     3781 tgatttcaac gtatcataag tgtcgactaa gaacacgcag tctttatggg ttttagcgta
     3841 ggccataaat gcttcataat catttccata agattggact aacgaatgag catgggtccc
     3901 gctagctggt atcccaaata tcttcccagc acgtacattg cttgttgcat ctgctccgcc
     3961 gatatatgcc gctctagttc cccagactgc tgcatccagt tcttgtgccc gtctagttcc
     4021 aaattccaat agtggatctt ctccgatgac agatttgata cgagctgctt ttgtcgcgat
     4081 caacgtttgg aaattgacca tattcaacag tgctgtttct actaattggc agtgcgctaa
     4141 aggcccttcc acttggatca atggttcatt tgcaaaaact agttctcctt cacgagcaga
     4201 acgaactgtt gctttaaatt caaaattttg taaatactcc aaaaaacctt caggatagac
     4261 ttctaatcca cgcaagtaag caatatcgga atcattgaac gtcagattct ctaaataatt
     4321 gactaaccgt tccaatcctg caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa
     4381 gtaacattca aaaacagcat gtaaatcagc tcgacctaat tcccagtatg tctgcatcat
     4441 attgatttga tatagatccg tatgcagcgt caaactgtcg tctgggtaag tattattcat
     4501 cttgtattac tcctcttggc aagttttcat ttcctaccca ttataacaaa aaaaattaat
     4561 aggaaaagaa atcttccatt ttttctgaaa aagcacatgt tttacggata tttcttgcta
     4621 aaaatgaact ccatctgcta aaactatcct ttcttattgg tatttaacct aaacgagaaa
     4681 gaatacttat tttattcaat gtatccgaga cacgaagaaa ttagcgtttt ctatttttct
     4741 tacttgataa aataagtaga ttttcctact acaatcgtag ccgttaagat accgatgatt
     4801 gaacaaatat aaccaaatgt ctctacaaca taatcaatgg caaaagtttt tgaagcaata
     4861 atacgtcttt ccttttttgc ttcttatccc caaagaaacg gatataactt attatgtatt
     4921 gaattttttt ggaaaggcga attatgacaa gatatacctt gcgttgaaaa aactcgcgga
     4981 aaaaatatca cacgttttta aaaagataca aagtaagaaa gcagctgtca atagaatgga
     5041 aacaaaaatg gcgagacatc aatcagtctc gccttgtttt ttattgtata cattagatat
     5101 ggagcaggtt accataaaga tgattattag tcgttattaa atagaatcct tcgtattttt
     5161 ttgttctatc actacaatat ctttatttgg acgtttgaat tgtattccgg cacgttctag
     5221 ctcctgtttc actcctacat ggactttgac atcttctcgt gtcattttta aaacgtgatg
     5281 aatcgcatag tcgatttctt gccttgtgat ttttttcttc gtattgatca tcacgacttg
     5341 ctttttatca tatgtgtcta ccaatagcag ttgtgttcga tctaacgaaa ataatttgta
     5401 ataattaata gccgtattcc caaaaaagaa ttccactact ttaggataaa atgtcttaat
     5461 atttaaatta ctattcacag gtgtttcgat tagcttcatc tcattcctct tttctatata
     5521 gtcgatttag atccaatctt aagttatgtt accatctttc ctagcattaa ggaatcggtt
     5581 acaattgaac ctttaaagac aaaaggattt accttttatc acttaatagt ataacataat
     5641 ttcatgagaa aaaaatacaa ttttttcttg accatctcgt tacgtaaagg attatgctta
     5701 catttgtcag gaggaaaaat aatggagtac acaataaaga aaatgtcaga gatatcagga
     5761 gtcagtccga gaaccttgcg cttttatgat gagattggac tgctaaaacc agctaggatc
     5821 aattcttcag gttatcggat ttatggcaaa aaagaagtcg atcgtctgca acacatctta
     5881 ttttatcgaa caatggcatt caagttggat gatatccaag aggtcttaga caatccatct
     5941 tttgaccacc agaaagcttt aatcaagcat cagcaaatgt tgctggaaaa acgggcacaa
     6001 atcgataccc tcttgacaac tgtccaacag acacttgata tgtacgaagg aggaagaaaa
     6061 atgagtgaca aagaaaaatt tgaagggttt aaacaacaaa agttgaaaga aaatgaagaa
     6121 tcctatggac aagagattcg acaaaaatac ggaaaaaaaa aggttacagc atccaatgaa
     6181 aaatggatga atatggataa agaaacgtat gaagaaatgc aaactgttga aaagcagatg
     6241 ttatttgatc tcactcttta cttaaaagaa ccagaagatg aaagcttagc agaccgtatc
     6301 ttccaagctc ataagaagtg gttatcttac agctggcctg aatatcagcc aaaagcgcat
     6361 aaaggactag gaatgatgta cgaagccgat gaacggttca ctgcatatta tgacgagcga
     6421 agtggtcatg gtgcagcaaa agctttaaat gaaattatcc aaaaaaaggc gtaatctttt
     6481 acatgatcca acaaaaaagt acacgtaaaa tgacctaata acaaaacagc agacacccaa
     6541 gttagatttt tatgctaact tttggtgtct gctccattct ttcacaagtt ttttcatcct
     6601 actttaaata tattcatcgt tgaataaacg tataaggtat tttcacttta tccttttttt
     6661 ctgtaagaaa caattgaaat gccatttctc cgcaagcttc cagatggtgg tcgatggttg
     6721 ggaaaccaag aagctcgctg atcagcaaat tatcgcgtcc aatcaccact ggtgtcttat
     6781 cttgcagata gctttgcaag atgcctgctg caaccatatc cgaatttgta aagacaccat
     6841 caactccaag ctctttaaaa aagaagcctg cttgttttcc tttctctgca tctatacaat
     6901 cccaaaaaac aagtgattca tcaaaagatg gaaaaagttg atgacatagc tgcaaagtga
     6961 tttttgagtt ataacttagc ctgccacttc gccccaacgt gatgcccaat tgctttatac
     7021 cttgctgctt caaataagta agaccttctg taattgattg ttctcgatca ataaagacac
     7081 agcttgcctg ttttcccttg atttcttcac aaaaaactag aggtccatat ttttgatatg
     7141 ccaataaatc atctagtgta ttggcacgag acgtaatgat caatccatcg aaagctttag
     7201 ctgcaaattc ttcaagatac cttcgctctt tttctttctg atagcccgtc ggtaaaagtg
     7261 tcactttgta atcataatca aacgctgctt caatgatccc actgatcaac tgatcaaaat
     7321 acggatgatt ggtataaggc agaatcactc caatgctttt cgtttttcca taacttaact
     7381 gtctggcatt tgcattaggg atataatcga cttctttcat gaccgcaagt atttctttcc
     7441 ttttttcctc atctacatat ggatgtccat tgattacacg tgaaatagta gaaactgaat
     7501 aacctgtgag ctttgcgata tctcgaatgt ttgccactgc tgcttcgcca cctatacaaa
     7561 aagattttct tgtaaatagt ctaccacagc tggaccgcat tcaaaaaact gatacatttt
     7621 tccttcatgc gaaaaactga tccgctgccc gccccaatat atatcttttg tcaaattttc
     7681 gatttcattc attggtattt cttttactat attcctattc aacccttgaa aactgtacaa
     7741 cgttaatacg gatcccttga actcgacaaa cgcataaaaa taattgttcg ttgtgccatc
     7801 tgttatctct acataagtat tcattgcaag ccctcctttt gatactttga gtatagtgta
     7861 tgaaacgcgt ctcatagcaa ggagtttgtt ttgccaaaga aaagttttat tcctctttca
     7921 ttattaccaa agtctcttat tttaaattga atacattata atgatactgt aaatatatct
     7981 ttattattag tgaatggata tttactttta atagataaaa tattataact attaaaaata
     8041 actattttca ttataattac aca
//
LOCUS       DACEPW010000115         7980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEPW010000115 DACEPW010000000
VERSION     DACEPW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.117"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="IW819_001964"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="IW819_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1245066.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1229..2332
                     /locus_tag="IW819_001965"
     CDS             1229..2332
                     /locus_tag="IW819_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1245067.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="IW819_001966"
     CDS             complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="IW819_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1245068.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3822..6269)
                     /locus_tag="IW819_001967"
     CDS             complement(3822..6269)
                     /locus_tag="IW819_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1245069.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6458..7894)
                     /locus_tag="IW819_001968"
     CDS             complement(6458..7894)
                     /locus_tag="IW819_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1245070.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2214 a         1736 c         1359 g         2671 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
      841 gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg ttcatcatga tatagattat
      901 cttttgcttt aaaaaaaacc aattatgaat tgacaaataa aacctgtttt catataataa
      961 tcttaatttt tagaattttt agataattac aaaaaagcaa agggaatcgg tagaaatact
     1021 aagaggaaca aaaattcatt attgtcgttt tcaatgtttt tgtacttgtt cgtttttagg
     1081 atacttattg atgatttcaa ggtgttcatg ccaccttatt catcacttat cctgtcttag
     1141 gttcacctct actccatcta cagccgcagt cagatcgatc actctttaaa attttaaccc
     1201 gtttcaacga agaaagaagg aaatgaatat gagtcaaagt gttttcttac caaattatac
     1261 cattggaaca gatgcgtatg aaaaagcaaa tggaatcgtc aaagaatatg ggacaaaagt
     1321 agtgctgatt ggagggaaga ctgcactttc aaaggcagaa aacaaaataa aagtagcttt
     1381 gacacaggaa atagaggttg tcgccagtct ctggtacggg ggtaattcaa cttatggaaa
     1441 tattgaacag ttgatccagc atgaaagtgt ccaaaaagca gacgtcctgt ttgcagtcgg
     1501 tggtggccgt gcttgcgata ctgtaaaagt cattggtgaa aaactagcta aaccagtctt
     1561 cacctttccc actattggtt cgaactgttc gcccgtcact gcggtatgtg tcatctataa
     1621 cgaagaaggc gtaatggacg gactcttctt tccttcaaaa ccacctgtac acaccttcat
     1681 tgatacagca attattgccg aagcacctga taaatactta tgggcaggta ttggcgatgc
     1741 tttatccaaa gaagtcgaat ctactttttc tgctcgtggc gatgatctag gctacacgga
     1801 tagattagga atccaaatcg gaaaaatgtg cattgatcgt ctcttgactt atggtacgca
     1861 agcattgaaa gatagtgccg acgatcgtcc ctcatatgca attgaacaag tcgtttcgga
     1921 tatcattgtt actaccggat tggtttctgt tttagttaac aataactata attctgctat
     1981 ggcacactca ttctattacg gtacgactgt tctagaaaaa atggagggat accttcacgg
     2041 agcatctgtc agttatggtg tactgacctt attgatgatg gatcagcagt tcgatttgtt
     2101 tgataaaatc tatgtgttta tgaaaaagaa ccaacttcca gtatcgttaa ccgacatgaa
     2161 tctcaatgtc acgaccgatt tagaagcaat cttagataaa gccatggcaa caaatgatat
     2221 taaacatgtg ccttatgaaa tcacccgaga aaaatttaaa caggccattc ttgacgtaga
     2281 aacttatcat gctacacaag aaagccagca aggatataag aaaatcagtt aaataattgc
     2341 taaaaccccc atcaagcagg ctgtcatcaa attgaggaca tttctttgct tgatgagggt
     2401 ttttcttgtc aaaaaaagct ttatagcttt cgctatagaa tcttttctct tatttaagtt
     2461 gaacagttcc ttaattaaga aaactgctcc ttttatggta attgaaaaaa cgagtaggat
     2521 acgattattt ggcaactgtt tttttcattt gcttgcttcg caattgtccg caagctgcat
     2581 caatatctgt accatgttct ttacgtatca cacagtttac gccgtttttc ttcaatacat
     2641 cataaaatct caatacatca gcttttggac tgcgagaata ttgatcatgt tcactgactg
     2701 gattatatgg aatcaagttg acatatgcta attttttctt atcttttaat agatctgcta
     2761 attgttgcgc atgctctggt ctatcattta cttgattcaa catgatatat tcaaacgtga
     2821 ttcgacggtt tgttttctct aaatactcat ccacagcttc catcaatttc tcaattggga
     2881 aacttcggtt gatccgcatg atcgatgttc gcacctcatt attcggcgcg tgcaaggaaa
     2941 tagccagatt gacttgcaag ccgttattag cgaattcctt gattttgtgt gctaatccac
     3001 tagtggaaac tgtaatgtgc cgagcaccaa ttgccaatcc tttggcatca ttgatgatat
     3061 gcaaaaagtc cattacattg tcgtaattat caaatggctc accgatcccc attacaacga
     3121 catgtgaaac gcgttcgcca agattgcgct catcaaaata atgttggacc atcatgatct
     3181 gtgcaacaat ttctccagca gtcagatcac ggttcttttt gagcagcccg cttgcacaaa
     3241 acgtacagcc gatattacat cctacttgtg tcgtcacaca aactgacaag ccatattctt
     3301 gtctcatcag tacagtctca atcatgtttt tatcagggag ttcaaacaaa tattttactg
     3361 taccatccga agcttcttgg acgaccatct gttttagagg attgatgata aaatgctccg
     3421 ataatttttc gatcaattgt ttcgatagat tcgtcatttc tgtgaaatta cttacacgtt
     3481 tttgatacaa ccattcccaa acttgggcag ctcggaattt tttctcgccg ttttctataa
     3541 accaattgat taattcttca ttcgtcaagc cataaatgga ctgtttttcc aaagtatttt
     3601 ccctcttttt ctcaaagttt cgctacatac tatacatgat tcatttcctt aatgcaatac
     3661 ttcacaaata aatcgaatag aactttaatc tcacaaaaac taaaaagaca agacatcata
     3721 ccgtattaga aaagatggaa aaaaaccatt ttttcttttg gtatcatcat gccttgtctt
     3781 tttattggtt attcgttttt caacccttca tcacacaagt tttacatgtc attatcgtcc
     3841 ggcaacgttt ctgatgtttc ttcctgctca atgaatactt tccgaggctt gcttccttct
     3901 gacgggccaa caactccatg agcttccagt tcatctacta aacgtgctgc tcggttgtaa
     3961 ccgatccgaa atcttctttg gagaagggaa atacttgccg tttgcatttc aacgacaagt
     4021 gcttttgctt cttcaaacag ctcatcttgt ggctgttctg gagcacctgt cgtttcaact
     4081 tcatctgtcg gcatcatttt ctcttcataa tgagcttctt gctgatctgt aacaaattga
     4141 acgacccgtt ctacttcgtg gtcagaaatg aatgctcctt gcacacggat cggtttattc
     4201 tcacccattg gcaagaatag catatctcct cgccctaaga gcttttctgc cccattagaa
     4261 tcaatgatcg tccgagaatc tgtaccgctt gataccgcaa atgccattct ggaaggtaca
     4321 tttgctttga tgattcctgt gataacatcc acacttggac gttgagtagc taggatcata
     4381 tgaatccccg ctgcccgagc catttgcgct aaacgaataa tcgcatcttc aacttcattg
     4441 ctggctacca tcatcaaatc agctaattca tctacgatca ctacgatgaa tggtaaggtt
     4501 ggatgtttcg ttccgttttc caagtttttc tgttgaacaa attcattgta accagaaata
     4561 tttcttactc cggttgccgc gaatttttca tatcgttctt ccatttcttg tactactttt
     4621 tgtaaggctt gcgctgcttt tctagggttc gtcacgaccg gcgtcaacag atgaggtatg
     4681 ccgttgtaca tattcaattc aaccattttc ggatcgatca tcatcagttt tacttcatgg
     4741 ggcttagcac gcattaatat acttgtgata atcccattaa ttgctactga cttcccacta
     4801 ccagtcgaac cagccacgag taagtgaggc atcttcgaaa gatcagccgt ttgtacccgt
     4861 ccagaaacat cacgtcctaa aggtacttct agtaatttat ctgggtggtt tggttgtgct
     4921 tcgatgattt ctctaaagga aaccatactg atctttccat ttggtacttc aataccaatc
     4981 agtgatttac ctggaatcgg tgcttccatc cggacatctt tcgccgctaa agcaagcgcg
     5041 atatcatctg ttaagttcac aattttactt acttttaccc ctacagccgg ctggacttcg
     5101 aattttgtga cggatggtcc taagctggct ttgacgacct tggcatcgac accgaaactt
     5161 tggaaagttt gttctaatac gccgatattt ttttcaattt ttttatactc atcactttga
     5221 tccacagttg gtatagaatc aagcaaatca actgtcggta attcatagtc ttgattttcc
     5281 tgttctgctt caatctcaaa ttctaatgag gtaccgtcat cctctggcaa ctcatctgaa
     5341 aaaccttctg ctgtattggt ctgttttggt gtatctggta ctggtttctc tagattcgca
     5401 ggatggatat tttcttgaaa actgtcgatt ggaacaaaac ttagctgctc cggctctgta
     5461 ttttctggtt cctgccattc atcagcagta ttttcccttt gctggctcac cgcttttttc
     5521 ttctcatatt ctgcagcttc tttttctgca gcttcttttg cagctaagcg ccttgcttct
     5581 ttcgcttctg cgcgctgttt cattctctct tcctttgccg cttgctttcg agcttgcttg
     5641 gcaggatcac cttccaataa ttcctgtact cgttctccaa ttgattggat accatttaac
     5701 agctgatagc cttccagatt actgaacagg aatgctcctc ctgccagtaa tagcactgct
     5761 gctaaataac ttccaggctg tgcaatcaag aagtaagtaa aatgataaag gattgctcca
     5821 atcatcccac caccaacgtt attgtctact tgactttgtt ttatatcact ggctaacaaa
     5881 tcccatgtcg tccccatgat atttggttcc ccagtatgga gtttgccaaa taaatgggca
     5941 tgtaacaata ataagatacc gaaataaaag agtatgccgc caaaccagcg tcggttttta
     6001 ccaattgaaa actctgtatt cttaatgaca atccaaaatc ctaatacggc taataaaaga
     6061 cagaggattt ggtaagtatt tcctgcaatg atccgaaagc cgtttgcaat caatattcct
     6121 aaaaaaccta attttaaaaa gccgaagaca gcaaataaaa tcaaggcaaa cccaacacca
     6181 atcgtcagca tttgttcctg ctgctgtttt tgctttttcg tttttttcgt ctttttcctt
     6241 tttttagtag gccgtttctt ttgtgccatt tattatccct catttcatca agtttcatta
     6301 tacaaaaaaa agtaagggaa ctaaagtttt gtttgaaaga actgacgctt tctttcattt
     6361 ttcagcttat tttacagttt ttcttaattt ctaaatgatt ttatcaagaa aaggcacggc
     6421 catatccagc cgtaccttct tgttttaagt atgtgaacta aaggttctct ccattttctt
     6481 caatcagttt ttggtaccag taataactgt ctttcttata tcgttccaaa gatccttctt
     6541 gtgtttcgtc ttgatcaaca tatacaaatc catatcgctt ttgataaccg ttcagccagc
     6601 taagcaaatc tgtataagac caggtgcaat agcctaggac ttctgctcca tccgaaattg
     6661 cttctttgat ggctttgacg tggctttgta agtaattgat tcgatagtcg tcatggattt
     6721 gtttatcttc tgtcaacttg tcatactctc ctaagccatt ttccgtaatc agtaccggca
     6781 tgcggtaacg gcttgtgata cgacgcagtc cgatccttaa tccttctggg tcaatctccc
     6841 agtcccaatt tgttcgttcg acaaatggat tctcaacttt tttatacatt ccaggaactc
     6901 cagtttcttc tgatgaacct ttttttcctg tcgtattcat ttttccagca ccaactccgt
     6961 ttaatggatt gaatgcattc gtcgccgttt gatagtaatt gatcccaaga aaatctggtt
     7021 tagcagattc tagcaattct gcatcgccag cttcaatcgt tggtgcccat cctttttctt
     7081 ctaagaacct catggcagca atcggatatt ttccccacat atagacatcc atccaataat
     7141 tggccatcag gtcttctgca ttttcagcag ccagaacatt ttcaggatta ctgtcaattg
     7201 gataatttgg cgtatacgca aagcttggtc cgatttttcc aggcatttct aactcgtgaa
     7261 atttattgat gacagatgca tttgctaaat tagcaatatg gttaacttca tacattcgtt
     7321 tcgggtctgt cacacctggc ggatgtgctg ctaacaaata acctaatgaa gtgaagatat
     7381 tttgttcatt caaactaatc cagtaatgga ctttcccgcg gaatgcctca aataatgttt
     7441 ctgcataatg agtgaaatct tcaatgatct gacgtgattc ccaaccatta tattcatctt
     7501 gcaatgcttg tggaagatcc caatgatata aagtcaacat aggttcaata tcgttttcaa
     7561 tcaattcgtc gatcaatctc tcataaaaag cgagcccagc ttgattgact tctcctcgtc
     7621 cattaggaaa aatccgtgtc caagctacag agaagcggta agctttcaga ccttgttctt
     7681 tcattaaagc aatatcttct ttataacgat gatagtggtc tactgccaaa tcaccgttcg
     7741 ttcctttaaa tgtctttcca ggtatccgta caaatcgatc ccagactgat tctcctttcc
     7801 catcttcttg ccaagctcct tctacttgat acgctgcaga agcagagccc cacagaaaat
     7861 cttttggaaa tgaggtaagt tctttgtgtt ccattcatct cttcctttcc tataaccttc
     7921 aatcgttcct cttttagaat aacacgaaac cccttccaca acaaaataaa attatgtaaa
//
LOCUS       DACEPW010000116         7968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEPW010000116 DACEPW010000000
VERSION     DACEPW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.118"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(449..1795)
                     /gene="gor"
                     /locus_tag="IW819_001969"
     CDS             complement(449..1795)
                     /gene="gor"
                     /locus_tag="IW819_001969"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1245071.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDTSSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1929..3170)
                     /locus_tag="IW819_001970"
     CDS             complement(1929..3170)
                     /locus_tag="IW819_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1245072.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3188..4111)
                     /locus_tag="IW819_001971"
     CDS             complement(3188..4111)
                     /locus_tag="IW819_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1245073.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4332..6824)
                     /locus_tag="IW819_001972"
     CDS             complement(4332..6824)
                     /locus_tag="IW819_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1245074.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6831..7298)
                     /locus_tag="IW819_001973"
     CDS             complement(6831..7298)
                     /locus_tag="IW819_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1245075.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7434..7507)
                     /locus_tag="IW819_001974"
     tRNA            complement(7434..7507)
                     /locus_tag="IW819_001974"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7471..7473),aa:Arg,seq:acg)
     gene            complement(7518..7603)
                     /locus_tag="IW819_001975"
     tRNA            complement(7518..7603)
                     /locus_tag="IW819_001975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7567..7569),aa:Leu,seq:taa)
     gene            complement(7620..7691)
                     /locus_tag="IW819_001976"
     tRNA            complement(7620..7691)
                     /locus_tag="IW819_001976"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7657..7659),aa:Gly,seq:gcc)
     gene            complement(7703..7775)
                     /locus_tag="IW819_001977"
     tRNA            complement(7703..7775)
                     /locus_tag="IW819_001977"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7740..7742),aa:Thr,seq:tgt)
     gene            complement(7793..7874)
                     /locus_tag="IW819_001978"
     tRNA            complement(7793..7874)
                     /locus_tag="IW819_001978"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7838..7840),aa:Leu,seq:tag)
     gene            complement(7882..7954)
                     /locus_tag="IW819_001979"
     tRNA            complement(7882..7954)
                     /locus_tag="IW819_001979"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7919..7921),aa:Lys,seq:ttt)
BASE COUNT         2180 a         1774 c         1317 g         2697 t
ORIGIN      
        1 atttgttcgg aaaaagtgac taactatgac acaatttttt tcacagtccc ttttttcact
       61 tgatttgatt cgtaccagcg aacttgacga gaactttctt ggtttggtaa ctatatggta
      121 ttcttttaac atcctcgttt gcttcttcta atttatttat acaaagtcac acttttttaa
      181 acatctgttc ttcgatacat ttctatttct tttaaacatc gataccaata ttttgaactt
      241 aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata atcaaaatct
      301 ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat aaataactaa
      361 aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt ttgttcaata
      421 aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct tccgctcctg
      481 ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc ttgatagcca
      541 cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag ccgacgattc
      601 tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa tcatttaatg
      661 caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg ccatattttt
      721 ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta aagacgactg
      781 tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa cgttctgata
      841 aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc acatcaccaa
      901 cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata aacccttttt
      961 tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt acacgtccac
     1021 cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc accgtatatt
     1081 cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt attccagctt
     1141 cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta agtggtcgtt
     1201 cttttcgata tgcccacatg acttcactac ctaaaccacg aaatacacct gaaagttcag
     1261 ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt aattcaaaaa
     1321 atccatttga atcaattgcg tattctccac caggaatggt cattttactt gcacggcctc
     1381 cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg atcgtttgac
     1441 tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct aatcctcttt
     1501 gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga ccggcgaaat
     1561 caacattttt taaatctgct tggataccgt aacttgaggt atctcgttca attgtttcca
     1621 aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg acacatgttc
     1681 cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct gctctgtttg
     1741 ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt tgcattttgt
     1801 tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt ctacagaaaa
     1861 caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg gatatatcag
     1921 ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg tgcgactggc
     1981 atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc ttcattatgc
     2041 ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc gatttcatct
     2101 cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc atcataagat
     2161 aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa taattgttcg
     2221 ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc tactttccat
     2281 aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat ataactgtct
     2341 acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt cattcttcct
     2401 tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc gatcattgac
     2461 atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt taaactgttt
     2521 cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga ttgagaacat
     2581 ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg accagaataa
     2641 gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt tttacgaatc
     2701 tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa cccttcttct
     2761 ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa atggatcggc
     2821 agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac aatcactgca
     2881 gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc atttccttca
     2941 tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc aaaagcaact
     3001 ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc atatgcattc
     3061 ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt ttccagtgtt
     3121 cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat ttctttcatc
     3181 atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct aattcacggt
     3241 tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct tgatcccagc
     3301 taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt acgaattttt
     3361 cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga taaagttcat
     3421 tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca tgactatctt
     3481 cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga cctctttcac
     3541 gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt ggacgtttgg
     3601 attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg agtttttctt
     3661 ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca actgcgcctt
     3721 ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca tcataaatat
     3781 acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta tcaccaactg
     3841 tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca tttttcattt
     3901 tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac ttgtgtgcta
     3961 attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc aatccaaatt
     4021 gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct gcttgttcaa
     4081 tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac ttcataatag
     4141 tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt tttgaaaatt
     4201 atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa aagagattgt
     4261 gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat tcagctacac
     4321 aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac attcaatgtg
     4381 atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc tccgccaagc
     4441 aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat tggacgtgca
     4501 ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg tgtgattttc
     4561 acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat ttggacgatt
     4621 gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat tcgattcaag
     4681 aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat ggctttgtga
     4741 tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat ttgtgtcgct
     4801 ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct acctttagaa
     4861 tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc tggatgtgct
     4921 ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt ttctgtcaac
     4981 tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg gcttgtactg
     5041 tatttttcca taaattctga catatcaaca cggatcaggg catcttcact tccaaacatg
     5101 acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc aaggaacatg
     5161 aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg gatcgcacgt
     5221 gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag tccttccagt
     5281 tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc agtccactcc
     5341 gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg ctcctgtttt
     5401 gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg tctgcgtaat
     5461 tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg tactagttca
     5521 tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc caaacggact
     5581 ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg agaagtgata
     5641 taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc cactccatgg
     5701 tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc aggagttggc
     5761 tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc gatatatttt
     5821 tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc taacgccggt
     5881 ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat taaggtatgc
     5941 aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat gacctttttc
     6001 attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc catatcaagc
     6061 atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat tttttgagct
     6121 aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac agggttattc
     6181 tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg accaacaact
     6241 gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga atctagcgta
     6301 ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg acgattcatt
     6361 ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg acgggctttt
     6421 gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc atcttttaat
     6481 aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt cacttcgctg
     6541 ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac tcctttaggg
     6601 taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat ttccgtttca
     6661 ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac cactaaggct
     6721 agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc ttcttgtgca
     6781 atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc tcacttccca
     6841 tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt atgatcttca
     6901 tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt tccttctttt
     6961 tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt tttctcactg
     7021 attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc tgaaatcctt
     7081 acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag ataaccacgt
     7141 tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca gttaaagagg
     7201 tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat tttctttata
     7261 tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc ctttgactaa
     7321 tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct ttttgttaaa
     7381 taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat cgacgcgccc
     7441 aagaggagtc gaacccctaa ccttttgatc cgtagtcaaa cactctatcc agttgagcta
     7501 tgggcgcata tttgaaatgc cgaggaccgg aatcgaaccg gtacggtgat cactcaccgc
     7561 aggattttaa gtcctgtgcg tctgccagtt ccgccacccc ggcgcgattg ctttggcaaa
     7621 gcggaaaacg gggttcgaac ccgcgacccc caccttggca aggtggtgct ctaccactga
     7681 gctatttccg cgtgtttaaa gatgccggct aaaggacttg aaccctcgac cctctgatta
     7741 caaatcagat gctctaccaa ctgagctaag ccggcatgta aaacttaaat tatgcgggtg
     7801 aagggacttg aacccccacg ccgtaaggcg ctagatccta aatctagtgc gtctgccaat
     7861 tccgccacac ccgcaaaaat atgagccgta cagggctcga acctgtgacc ctctgattaa
     7921 aagtcagatg ctctaccaac tgagctaacg gctcatatgg aggttaac
//
LOCUS       DACEPW010000117         7924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEPW010000117 DACEPW010000000
VERSION     DACEPW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.119"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            589..2127
                     /locus_tag="IW819_001980"
     CDS             589..2127
                     /locus_tag="IW819_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1245076.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2124..3089
                     /locus_tag="IW819_001981"
     CDS             2124..3089
                     /locus_tag="IW819_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245077.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3076..4398
                     /locus_tag="IW819_001982"
     CDS             3076..4398
                     /locus_tag="IW819_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1245078.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4461..4565
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4639..5382
                     /locus_tag="IW819_001983"
     CDS             4639..5382
                     /locus_tag="IW819_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1245079.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5397..6389
                     /locus_tag="IW819_001984"
     CDS             5397..6389
                     /locus_tag="IW819_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1245080.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6401..7138
                     /locus_tag="IW819_001985"
     CDS             6401..7138
                     /locus_tag="IW819_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245081.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7128..7850
                     /locus_tag="IW819_001986"
     CDS             7128..7850
                     /locus_tag="IW819_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1245082.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2599 a         1349 c         1701 g         2275 t
ORIGIN      
        1 ataaagcaaa tcaatttatc tttttatttg aacacggcaa agcaccactt gctatgaaag
       61 acatctttct ttttttgatg aagaaccgtg agaaagctgt ttattatcag tcaaaagaag
      121 aactcattcc tctttatgga gtgaaagaga tagaaaataa agtggtgata taagtaaaaa
      181 taagaaaaca agacgtttat gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag
      241 tgtcatgata cagtataata ttcgttgaac aggccaataa aggtgttctc ccccacgaga
      301 tttctacttt attggctgtt tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata
      361 tagctgattc caagtgacaa tttcgtatag atttttatgg tattcgtagt gaaaaaacga
      421 gaaccaaaaa gttgatccga ctttgttgct tggattcttt ttttgtcagc atatttagag
      481 gtattttttt cttttttttg ttcgtgctgt aaggttaagg caagacaagt tagttgggaa
      541 aaaggaaaga aatgtttata cttaaagaat aacagaaagg aaggtcgcat gaataatttt
      601 atacaaacat taagtgaaag aaaaggcgag ttgctggaag ctgcatttca acatctgtcc
      661 atttctctca tttctttgct gattgcagca ctgattgcta ttcctttagc gatttgggca
      721 gccaatcata aaaaaatggc agaagtctta ttgcagatca cgggtgtgct ccaaacgatt
      781 ccttcacttg ccttattagg tttacttatc ccatttgtag ggatcggaac tgttccagca
      841 ttgattgcac ttgttattta cgctttgctt ccgattttcc aaaacacgta tattggtcta
      901 gctgaaattg acccgtctat tgaagaagca gcggtagcat ttggtatgtc cagaatgaga
      961 cgcttgctga aagtagagct tcccatcgca ttacctgtca ttatctcagg tatccgaact
     1021 gccttagttt taatcatcgg tacggctact ttggcagcat tgatcggagc aggtggatta
     1081 gggacattta ttttgttagg tattgaccga aatacacctg ttttgacatt gattggtgcg
     1141 atcagttctg ccttgcttgc aattatattc agcggactga ttcgctggtt gcagcataag
     1201 aaacctcgat attccatcat tactttagca gcagtggtgt gtttatttgg cggcgtttct
     1261 ttatatcagt ctgccttgtt taaaaatgaa accatcacga ttgctggaaa attaggagcg
     1321 gaacctgaaa tcctgatcca aatgtataaa gaattgattg aagacaacac aaaaacagaa
     1381 gttgaattaa aaccgaattt tggcaagacg agttttttgt tcaatgcact ggatcaaggt
     1441 caaatcgata tctatcctga gttcacaggt actgtcctag aaagcttggt gaaggtacct
     1501 gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat atgacgaagc agaagaatta
     1561 ttgaataagc aattcgatat gacattatta aaaccaatgg cttaccaaaa tacgtatgcc
     1621 ttggcagtca agagatcttt tgcgaacgaa catcaactta aaaagatttc cgatttggca
     1681 gctatctctg atcagatcaa agcgggattc acactggaat ttattgatcg ttcagatgga
     1741 tataaaggaa tccaagaagc atatggcttg aatttttcaa gtgtacaaag catggagcca
     1801 agtttgcgtt accaagcaat caataatgga gatgtcaata ttgtggatgc ctattccaca
     1861 gatagccagc tcaaggagta cgatctagtg actctagaag atgatcgagg gttattccca
     1921 gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt
     1981 aaagcactga ataaattaag cggaaaaata acagaggatc aaatgattga tatgaattac
     2041 caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa
     2101 ggattagtaa aggaggaaga tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa
     2161 agtatgatgg aaagtatgtc atcgatgacc taaatctggc aatcaaaaaa ggagaaatct
     2221 ttgttttggt tggtccttcc ggtagtggaa aaacaactac tttgaaaatg atcaatggat
     2281 tatctaaacc atcagcagga gacatctact tcaaaggaaa atcgttaaat gaatataatc
     2341 tgcagaaaat gcggtggaat atgggatatg tgctgcagca aatcgctttg ttcccgacca
     2401 tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa
     2461 gagcagaccg tgtagatgaa cttcttcaaa aagttggctt atctcctgat atttatcgag
     2521 atcgtatgcc tagagagttg tctggaggag aacagcaacg gatcgggatc atccgagcta
     2581 ttgctgcaag cccagatgtg attttgatgg acgagccatt tagcgcatta gatccgattt
     2641 caagaaactc attacaagaa ttggttttgt ccttacacga agaattgggc acgaccatcg
     2701 ttttcgttac gcacaatatg gaagaagcaa tcaagctagg tgatcgtatt gcatttatga
     2761 aggatggaga aattatccaa tgtgacacac cagagcagtt attgatgaat cctaaaaacg
     2821 actacgttcg gcatttcttt gatgaaccga aacagacgaa agagtggcga gtagaagatt
     2881 tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct
     2941 ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt gtatccaagc attgtgatag
     3001 tagaaaagca aagaagtatt gggtcgctga caagtaaaga aatctttgct tttttaagtc
     3061 gagaggagga aggaaatgaa aacatatgat gcaatcatca tcggttcagg tgtgagcggc
     3121 ttaagtgctg cttatggatt gaaagaagca ggtaaaacag tactagtggt cgaagaagat
     3181 ctatggggcg ggacttgtcc taaccgtgga tgtgatccta aaaaagtctt gttaagtgca
     3241 gtagaggcac gtaatcgggt aaaacaattg agtggaaaag gttttaacga gatcccaact
     3301 gccaactggg aagaacttca aaaattcaaa cgaacattta cggatccagt cccagaaagc
     3361 cgaaaaaaac aattagcaga agctgaaatc gatcatcttt caggaaccgc tcgttttttg
     3421 gatgattcat ctattgaagt caacgaagaa gtcttccatg cggattactt agttttggca
     3481 accgggcaaa gacctacgat cttaccagtg gagggaaagg aatacttgaa aactagtgca
     3541 gactttttat cattgcctgt actgcctaaa gaaatcattt ttatcggtgg tggttatatt
     3601 gcttttgagc tagctacgat tgccaatgct gcagggagca aagtaaccat tgtccatcat
     3661 aatcaacggc cattaaaaga atttgaggcg tctttggtag aggaagccgt ccatcaaatg
     3721 gaagcatctg gcattcagtt tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt
     3781 actcgctatc gtttagtcgg aaaagagacg gaattagtag cagatatgat tttttgtgcg
     3841 actgggagac agccgaatac agaatcttta gcacttgagc aagcgaatat cgtctttgat
     3901 aaacacggaa ttgcagtcaa tgattatctg caaacgagca atccaaaaat ttttgcgtgt
     3961 ggagatattg tttcaaggaa aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat
     4021 tatgtagcta agcggataac ggatgcaaca agcgaaccta tcaaatatcc gattatacct
     4081 acgatcgtct acgccagtcc taaacttgct gaagtaggag ttacaaaaag tcacgcctct
     4141 tcgtcagatc aagtggtaga gatggatctt accagttggt tcacctatca tcgtgtcaat
     4201 gaaccagttg ctaaggctga attgaccttt gaccagcaaa attatttgat tggtgctgca
     4261 gtgattagcg agcaagcaga cgaattgatc gatgatttga cactcgtcat caatcaaaaa
     4321 ttgactaaaa aagagttgga tagctatatc atggggtatc cgacattggc aagtgatctc
     4381 tcctatttat tgaaataatc agcatgataa ctaaagcgct tgttgctttt tccttcttgt
     4441 gctacacttt aaaaaatgaa tacctgtttc tggggatgtc gtagacgaca tgagagtaag
     4501 ttgtgaactt agacccattg aacctgtcag ttaatactgg tgaagggaga aacatagcag
     4561 acgcttgaag cgggttacta tgtcgtgccc atttcaagcg ttttttgtta ttcatattag
     4621 aagaaaggag gtaacaaaat gaagaaaaat ctttatccag cagtcagtat ggcattcatt
     4681 ttaattattt ggcagattgt tgtcatagtt tggaaaatac ctgcctttat tttacccgga
     4741 cctgctgctg tattttctgc attagcggca gatgcacaag tattactttc ccattctgtc
     4801 acgacattaa atgaagcagt gcttggtttg gtaatagcta gcgttctttc ttttattact
     4861 gccttggcaa tggattattg gaagttttta gaaatgtctt tttatccgtt actagtgatt
     4921 tcacaaatgt tgccaatcat ggtgttaggg ccattactta ccctatggtt tggtttcggt
     4981 atgcttccaa aagtgatcct tgttgtcttg ataagctatt ttccgatcgt agtttctttt
     5041 tcggatacat tgaggaaagt atcaaaagag caaatcatct ttttgaaaac gatgggggca
     5101 gatacaggga agatttatcg aatatataaa ataccagcgg gtttatccgg cttcttttcc
     5161 ggtttaaaag tggctgctac ttattgtgtc agcggagcag tcgttggaga atggctaagc
     5221 gctcaagcag gcttagggta ttacatgata cgcgtaaaaa acagctacca gatcgataaa
     5281 gtttttgctg cgatcatttg tgtcattcta ttgagtttat tgctgaatgg cgcttgttca
     5341 ttattgaaaa aagggtatta tcgtatgctt tatatttttt aagagaaggg gatcaaatga
     5401 aaaaattatg gcttttatta ccgctactgt tgcttttatc cgcttgtggc actgcaaaag
     5461 aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt gacacttgtt ttggattatg
     5521 taccgaatac taatcatact ggcatctacc tagcaaaaga aaaaggctat tataaagagg
     5581 ctggtttaaa tgtccaaatc atcgaaccag gagataattc gacaagtatt ggtctagtag
     5641 gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag
     5701 atggacagaa tataccagtc aaagcaattg ctacagtgat caaacataat acttctggat
     5761 tcgccaccct atcagacagt aacatccatt caccaaaaga ttttgaagga aaaacatatg
     5821 caggttggca aagtccaagt gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg
     5881 gggacttttc aaaactgaca atggtaggaa gcaatggaga agggccagaa agcttaggaa
     5941 aatcctcaga tatccaatgg tactttgaag gttgggatat gatcaaagca aaagaagcgg
     6001 gtattgaact aaactatatc ccattgaaag aattagatga acggttagat tactatactc
     6061 cggtaattat tacgaatgat caactgatca aaagtgatcc agaattggta cagtctttta
     6121 tggatgccac gaaaaaaggc tatcaggaag ctattaagga tccaaatgac agtgctaaac
     6181 tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt agaagaatca caagcattct
     6241 tgtcaaaaaa ttatacggat gatcctgaga attggggatt gatggaagaa aaagtttggc
     6301 gcaattatac agcttttatg caggaaaacg gattgatcaa gcaagatgta ccttcgcaag
     6361 aactattcac taatcagttt ataaaatagg agtgatagat atgtcaatcc aaatcagcca
     6421 cttgaatgta gtcattgaaa acctgaaaat attagaagac atccatttca ctatccctaa
     6481 agataccttt acatcgattg tcgcgccaag cggagccggt aagtcaacat tgctgaaaac
     6541 tttaacagga gtccagccta tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac
     6601 tggtctccat acagcattta gttatatgcc gcaagaagat atgttacttc cttggctgag
     6661 cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat ctaacaatca atgaagaaca
     6721 agtaagggat tatctggata tctttgggtt aaacggctat gaacattttt tgccggaaca
     6781 attatctggc gggatgaagc agcgaacggc cttgttacga acaatcatga atccttcttc
     6841 ttacttgctt cttgatgaac catttggagc gttagatgct atgaccagag ggcagatgca
     6901 agactggatg ttgaagctgc ctaaaaaagc aaagcggact actttacttg tgacgcatga
     6961 tatcgaagaa gcaatttatt tatcagacag gattcttgtg ctttccgccc gtccggcaca
     7021 tgtgattgct gagattcaag tgcctgaaaa acgacggtca cgtgaatggc tactgcagca
     7081 atcagagttg aagcaaacga tttatcagtt attggcaggt gaattagatg ttaagtgatg
     7141 ctcattgtca tttagatggg agtcagagtt tagcactcct tcagcaggaa cacacgattt
     7201 tgacgatcat caattgtgat tcgccagaag aatggaagga aaacagacag cttgcagcaa
     7261 gtaagactca agccttaagt tacggtatcc atccttggaa agcagatagc tatacgtttg
     7321 aacaagttga gccttttttg aaaaaagcaa ggatcatcgg tgaaataggg ttagataata
     7381 tttggacgaa tgtaccgatg actacacaaa aaaaagtatt tgagcgacag cttgcttttg
     7441 cagccataaa tgagaaacct gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa
     7501 attatatcca agcttaccct aatcactatt tgatccattg gtattcttct ttggactatc
     7561 aacaagacta tattgattta ggctgttatt tcagcattgg tcttgatttg aaaaagaatc
     7621 cagcagtctg gcaactagct aaagcagtcc caatcgacca cttacttatt gaaacggatg
     7681 gactacatgc agcagagtgg gctttagggg ggattttttc agaaaaagat tatgtctcac
     7741 tgcttgaaga acagttaaag acaatcgctg agatcaaaga gctatcagtt gaagaagtta
     7801 aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt aggtaaataa aaaagagatg
     7861 tgacacaact agtgtcgcac ctctttttgg ctttctttgt ataaaagtaa actgtttgat
     7921 taat
//
LOCUS       DACEPW010000118         7791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEPW010000118 DACEPW010000000
VERSION     DACEPW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.120"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..391
                     /locus_tag="IW819_001987"
     CDS             107..391
                     /locus_tag="IW819_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1245083.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            394..1311
                     /locus_tag="IW819_001988"
     CDS             394..1311
                     /locus_tag="IW819_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1245084.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            1304..2086
                     /locus_tag="IW819_001989"
     CDS             1304..2086
                     /locus_tag="IW819_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1245085.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            2087..2767
                     /locus_tag="IW819_001990"
     CDS             2087..2767
                     /locus_tag="IW819_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1245086.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            2760..3656
                     /locus_tag="IW819_001991"
     CDS             2760..3656
                     /locus_tag="IW819_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1245087.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGIYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            3851..5500
                     /locus_tag="IW819_001992"
     CDS             3851..5500
                     /locus_tag="IW819_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1245088.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            6094..7029
                     /locus_tag="IW819_001993"
     CDS             6094..7029
                     /locus_tag="IW819_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245089.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            7022..>7791
                     /locus_tag="IW819_001994"
     CDS             7022..>7791
                     /locus_tag="IW819_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1245090.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLLFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         2408 a         1348 c         1718 g         2317 t
ORIGIN      
        1 gaagaaggga ttgtgaaaag tcttttgtca taacctctta tttggtagct aattgtaaat
       61 cccaaacggc atgtattatt tttcataagt ccgtttttaa ggattaatgt tttcttttgc
      121 tttttatatg gtctggcttc agcgagaagt tccaacgcat atacaaggac gtctgatgtc
      181 aacgataaca acgattacca tggtggccag tccacttgga ttagcactgt ttggtgcgct
      241 ctttgattgg cagacaactc aaccacgtct gcgagacagt ctgatttttc ttgaaattca
      301 attggaaaat ttacactttc tttatacatt tctggctgct ttctttatac ccgtcaacta
      361 tacttcactt atcaataaga aagaaggata agtatggatt ttggaaatga aacggtcttg
      421 aaagcaactg gtataaccaa aaaatacggt gctgccaaag cattagataa agtatcgatc
      481 gaaatcaaac gtggaatgat ctatggtttg attggggaga atggagcggg taagtctacc
      541 tttatgagaa ctattatggg tttgatctcg attgatgaag gttcgatcga attatttgga
      601 acgacggatt tgcaggcagc aagacgacgt atgggtcaat cgattgaaac ccctgctctt
      661 tatccagaat taactgcaag agataaccta cgtatccaag cagcgaacgg cggtgtgtcg
      721 gatagagaaa tcgaagacct gctgaaaatg atgcgattgg aaaatacagg gaaaaagaaa
      781 gcaaagaatt tctctttagg tatgcgccag cgtttagcta ttgcaaatgc actgatcacg
      841 aatccagaat ttttgatttt ggatgaaccg acgaatggga tggatccagc agggatggca
      901 gaaatgcgag aaatcatcca acgtttggta aaagaacggg gcatcaccgt cttgctttct
      961 agccacttgc tagatgaatt gtcacagatc gccactcact acggtatttt gcatgaagga
     1021 cacttgatca aagaattatc gaaagaagaa ttggcacaag aatctcgtca atttataaaa
     1081 attgacactt cggcgacgga acaagcagta acggttctcg actctcttgg ttatagggat
     1141 tattttgtcc aatctagccg tgttatccag ctttttgaag gaattgacca agtagcagcc
     1201 atcaatcaag cattggtaga ggcaaaagtt cctgtggatg gtatccacct agttgggcaa
     1261 aaactggaag attatttctt acaattaact ggaggcaatc cacatgttta atctattaac
     1321 tgctgaaaaa ataaaagtat ggaagagccg taaaatgtgg gtctcactag gactgatggt
     1381 cgtacttccg cttttttatt cttggaacga atggtatatg catacaaaat acggcaatga
     1441 attaaaccaa gcaacggata cagtcatcaa cggtgcaaca gggattttaa tggtggaaaa
     1501 aatggctgca tggattcttc tggctttcgc agcatttgcg tgcttttata ttggggaaga
     1561 atttcaaaac ggtacgattc gcaatacgtt atctttagga cggaatcgaa tgacttatta
     1621 tgtgtcgaaa cttctcgtta cgcttttgat cacaacactt ggcgtggttt tgattacggg
     1681 gctagccatg attgcttaca cacttgcttt cggatttggt gaagttgagg gaatcaaaaa
     1741 ctatggaaat tatgttctaa aagtatttcc tgtattgctt ctgctgatat tagcgacatt
     1801 atctgttcca gtggctttga cattcatcac tagaagtacc agtgtcagtt tgcttctttc
     1861 ttttctttat atcatgggaa cggccttcgt tcccggtgtt ttcgcaaaaa tcaaaggctt
     1921 ggaatttcta acggagtggt ttacggaaac gtggctcatg tatacggact ttgcgcaaca
     1981 agcaacttat tcgcaagcac cgaaaatgat tctggtcagt ctagtaacaa tcgtggtatc
     2041 atcagctcta ggaatgttta ttttccaaaa atctgacatt aagtaggtgt tcctgctgga
     2101 aactattttg attatcgaag acgatgaagc tgttcattct ttactggagg aggttcttga
     2161 acagagatac aaacttcttg atgcttattc aggtacggaa ggaaaattat tgttaagcac
     2221 atatcctgtc gatttgatcc ttctagatct catgcttcca gggctttcag gtgaagcatt
     2281 gttagcagaa atccgacaga catcgaatgt tccgatcatc gttctttcag caaaaagcga
     2341 tcaaagagat aaagtttcct tgttggcagc aggagcggat gactatgtga cgaaaccatt
     2401 tgatatcgaa gaactgttgt tacgaatcgg catccaactt cgtcatcaga cttctgtcca
     2461 agtaaaagat ctgtcgcaac gcatctatta caaggaaatc ttactggaca aagagtcgag
     2521 ggatgtaaat gtgaatggtg tggctgtcca tttgacagga agagaattcg atctgctgaa
     2581 gcttttttta gaacatccga aaaaagtatt ttcgcgagca aatctttatg aaacagtttg
     2641 gcaagaacca tattttgata gtgagaaaac agtgaatatg catatttcca atcttcgagg
     2701 gaaactagca aaggcacatg ctgtgtatat tcatacggtg tggggagtgg ggtttagatt
     2761 tgattaagtg gctgttgctt ctcgttgtta tctttatggg aagttttatt ttcttcctgc
     2821 aaagggaaat caagagatta actaagaaaa tcgccactct ttctgaacga caagaatatg
     2881 gtggtcgtct ttcagtcgaa tttcgagaaa agaatctaat ggacctagta gatgcactga
     2941 atacatgggt ggatgaaaat gaacaaaaag gacaagcatt tcaagaaatg gaagaaaaca
     3001 tcagactatc gatcgtcggc ctttctcatg atctcagaac tcctttgact gctattaacg
     3061 gttatgtaca attactggaa caggtagagg atacggagaa acgggagaag tatttaacga
     3121 tcatcaaaca atctgttgca agattactca gtatgacgga tcagttttat gatcttgctc
     3181 gaatagagac gaagcaaaaa gaaatggatc tccatacgct ttctttttct aaagaggtgg
     3241 aagaagtatt tttctctttt tatgaacaat tttctgagaa aaatatcgag gtagcgttcg
     3301 ctgaacactc gataaataca actgtattag cagatccgct catgttgctg cgagtgctgc
     3361 aaaatatcat acagaattta ttgcgttatg cagatggaca agcaaaggtg acattttcaa
     3421 aagaggattc attcattcga ttaacagtgg ccaatcctat caaaccagga gcgcaaacct
     3481 ctgttgaaaa agtcttccag cgtttttata ccgaaaatac cagccgcaca aatacggaat
     3541 ctagcggaat cgggatttac ctgtcaaaaa aattggtgga aggaatggga ggagagatga
     3601 ccgctagatt agacgggggc atcttctcca tctccgtcaa gttgcggagg gtttaattgg
     3661 ctctaattta cctgaagtga atgacaaaaa attgtatctc gttttaaaga catcttcttt
     3721 tttggaaggt gtttttattt tggtgtgatt gatacgaatt aggtaacgaa gacagttatt
     3781 tttgtacccc cttttcaaat agcgcaacag tgctatacta aaactaacaa atcaaggaac
     3841 ggagagggat atgggccaaa gagaatttat cgttttaatt attattgcag ttgttatttt
     3901 attagttgtt ttagacattt tttcacgttt gaaagtaaag gaaacagttc gtagtaattg
     3961 gggaaaaatt ccttatcaac cgagatttga taaagaagaa agtttgaagg atgcgtggct
     4021 cacggagaaa aaattccgtt catgggatag tgaaatcgac gatcttactt ggtatgactt
     4081 ggatatgttc gaagtatttg aagggatcaa cagtacctat tctagtgtag gttcagaagc
     4141 gttgtatcag cgtttacgta gctttgattt tggagaagat tatcagttag aaaaactaat
     4201 tgctttttat caggaaaatc ctcaactgag agaaagaatc caatatcaat tcgctcgttt
     4261 aggaaaaaaa gatcataatt ttgcaaaaca atatctagcg gatggaaaaa gtaaaaagat
     4321 tggacgcgta ggtttattta tttttcttgg cttgcttccg ctggtgggtg taggtttgct
     4381 tcttttaggg caagtactag gaatctatct agcaatcggt ggcttagtat tcaatacggt
     4441 ctattatcta gtgaagaaac aaacactaga aacagagctg aatagcatga gttatttggt
     4501 ttcaacgatt tcagccgcca atcagttagc aaaaatgacg acaccacttc aaaaagagct
     4561 gaaacagcaa actaaaccat taagagacat ccctaaatta ggtttttcat ttcgaatgaa
     4621 gagcgggagt gaagcagaat tgatttttga atatgtcaac atcatgttca tgctgccatt
     4681 tatctcctat catttcgttc tcactaaatt agaaaagcat tcaactgaag ccatcaagct
     4741 gtgggaattg ctgggagaat tggaagttgc agcagccgtt ttgaattatc gcacctatat
     4801 gccgatcact tgccgaccag agtttactga gggcggtgtg acagcgaaag atatctatca
     4861 tccattgtta aaagaagcgg tagttaatcc agtcaactgg cagaagaata cattagtcac
     4921 gggttccaat gcatcaggaa aatctacgta tgtcaaaagt atagccatca gctgtattct
     4981 agcacagacg atccaaacag cgattgcaga acagtttacc ctgcaacctg gacatgtgtt
     5041 gacttccatg gcaatcgaag atgatttatt tgaaggcgac agttattttg tttcagaaat
     5101 caaatcgatc aaacgtttgc tggaccaagt ggcttccaaa gaacgctgct actgcttcgt
     5161 agatgagatt ttaaaaggaa caaatacgat cgaacggatt gcttcttccg cttcagtcgt
     5221 tcggtggctt gcggagtatc catctttagc atttgtcgcc acacatgata tcgaattgac
     5281 agagatttta aagaattatt gtgagaatgt ccactttgaa gaacaagtaa cagaaggaaa
     5341 aggaatcagt tttgatttca aattaaaaga aggaccttca aggacccgaa atgcgattgc
     5401 cttgctgaaa gttttgaatt atcctaaatc catagttgaa caggcccaaa aagaaagttt
     5461 gttgttcgat gaacagcgtc agtggtatcc gtttgattaa ctttatcaaa cgaagcatga
     5521 tgcgtgggtc caaaaagacg attatcaaaa gaagtacatt gatgccaaaa aagtggcaga
     5581 attcaatggg aacgagtctg ctgtccagat gatcttggaa gatttagcaa aagacgatac
     5641 aaaataaacc acgctgtctt ccttctagca gcttttatcg aaaaaactta gcatcagcga
     5701 cgagacgcac gttccatggc aaagttctcc gtaaaacaaa cagcgaaaac agaaattgaa
     5761 aataactatt tccattttat ttatttcggt taaaataaaa gaaaaaagct ttcgataaaa
     5821 ggacggagga aagcttaaca tgaataaaaa agacaagaac ctcagttata aggatcttct
     5881 atatgtcatc gtagtagttg ctgtcggtac ttttatgatt ttaaaagttc cagagttgtt
     5941 tagatagtta ttctatcgaa gatgatcatg aactagaaaa cacatctcct ctttgttagc
     6001 aggtgtgttt ttgtgtggaa gccaagttgt tttttcatct gctgtattcc atgatacaat
     6061 taagaatcat gtctataagg agataggaaa taaatggcaa atgcgcttga aataaataat
     6121 ttgaaaaaga tctatggaag tggggtcgaa gcattgcggg gaatcgatct ttcggtgaaa
     6181 gaaggagatt tctatgcgct attagggcca aatggagctg ggaagtctac aacgatcggg
     6241 attattactt ctttagtcaa taaaacatct ggaaaagtga atgtcttcgg ttatgatctg
     6301 gacaaggatc tagttcgagc aaagcaacag attggattgg ttccacaaga gttcaatttt
     6361 aacccttttg aaacggtcca gcaaatcgtc gtgaatcaag caggctacta cggtgtctct
     6421 cgcaaagaag cgatgaaacg cagcgaaaaa tatttgaagc agtccaatct ttgggaaaag
     6481 agaaatgtcc gtgcacgtat gctttccggg ggaatgaagc gaagactgat gattgctagg
     6541 gcattgatgc atgaaccacg tttgctgatc ttggatgaac caacagcagg agttgacatc
     6601 gagttgagac gcgagatgtg ggagtttcta cgagaattga acgaaaatgg tacaacgatc
     6661 attttgacga cgcattatct agaagaagct gaaatgcttt gtcgcaacat cggaatcatc
     6721 caatcgggtg aactgatcga gaatacaagt atgaaagagc ttctgtcaaa actgcaattt
     6781 gaaacattta tctttgattt agcgccatat gataccaaac ctgtgattga aggatatgct
     6841 tgtacatttg aagatgagca gacaatcgct gtcgaagttg aacgaaatca aggggtgaac
     6901 ggaatcttcg atcagcttac aagacaagga atcaaagttc tttctatgag gaataagtcg
     6961 aatcgtttag aagaactgtt cttgaaaatc acagaagaaa ataatcatac ggaggagaaa
     7021 aatgtttaat ctttatttta ctgcattaaa aagtttagct atcaaggaaa cgaaccgcta
     7081 tttgcgtata tgggtccaaa cactggttcc gccagtgatc acgacatcct tgtattttgt
     7141 tattttcgga aaaatgatcg gtgggcggat cggtgacatg ggtggttttt catatatgga
     7201 attcatcgtt cctggtttga ttatgatgtc tgctatcact agttcgtatg ccaatgtttc
     7261 ttcgtccttc ttctctcaaa aatttcagaa aaatatcgaa gaaatcttgg tagcacctgt
     7321 tcctacgcat gtgattattt ggggctttgt gattggtggt gtgggtcgca gtattctcgt
     7381 cggctctctc gtaacgatca tttccttgtt atttgtacca cttcatgtgt attcatggtt
     7441 catggtcatc attacattat tgatgacggc aatcttgttt tcattagcag gactgctcaa
     7501 cgggattttt gctcagtctt atgatgatgt atcgatcgtg cctacatttg tcttgcagcc
     7561 gttaacttat ctaggtggcg ttttctatgc catctctatg ttaccgccct tttggcaagc
     7621 tgtttcaaaa gtcaatccga tcgtttatat gatctccggt ttccgttacg gattccttgg
     7681 aacgatcgat gtgccagtag caacatcgat gatcgtactt gtgttattca tcgttgtttt
     7741 gtacgccgtt acttggtatt tgatcaataa aggaagagga ttgagaagtt a
//
LOCUS       DACEPW010000119         7738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEPW010000119 DACEPW010000000
VERSION     DACEPW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.121"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..131)
                     /locus_tag="IW819_001995"
     CDS             complement(<1..131)
                     /locus_tag="IW819_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245091.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDK"
     gene            complement(148..2382)
                     /gene="pcrA"
                     /locus_tag="IW819_001996"
     CDS             complement(148..2382)
                     /gene="pcrA"
                     /locus_tag="IW819_001996"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1245092.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            2537..4096
                     /locus_tag="IW819_001997"
     CDS             2537..4096
                     /locus_tag="IW819_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1245093.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(4207..6138)
                     /locus_tag="IW819_001998"
     CDS             complement(4207..6138)
                     /locus_tag="IW819_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1245094.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(6156..7070)
                     /gene="pfkB"
                     /locus_tag="IW819_001999"
     CDS             complement(6156..7070)
                     /gene="pfkB"
                     /locus_tag="IW819_001999"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1245095.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(7067..>7738)
                     /locus_tag="IW819_002000"
     CDS             complement(7067..>7738)
                     /locus_tag="IW819_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1245096.1"
                     /translation="LLNASESTIRRDLQELENSGFLERVHGGAKKEQLLNFEQNMSEK
                     SLKNVHEKQQIAKSAAETINDEDVIYLDAGSTTLEMIPFLQGKQITVVTNSVQHAANL
                     VDLELATIILGGTIKLATNAVLGSNAIEQLKNFHFNKAFMGMNGAHLEQGFTTPDPEE
                     AAVKRLAMKNSEETFVLIDHTKFNQLSFTSVAPLEAATIITERCPLEFVNEFREKTTI
                     KEAIQ"
BASE COUNT         2016 a         1682 c         1463 g         2577 t
ORIGIN      
        1 tctttatcat aaacatagtc ttccactgtt ggtttatctt ctacgtagta ttcgcgagac
       61 cattggttca gtcttgtacg aagttcttct gctcgtttag cagcttcgtc aatcgtcatt
      121 tcatgttcca tcatttaccc tccactacta agctttttca attggcgcaa aggcagccaa
      181 aagacgtttt actccttgac ttgggaaagc cacatctagt tctagatcct tcgacgtccc
      241 gcttactcgg acgactgttc ctagacccca ttttttgtgt ttgactttat ctccaggttt
      301 ccagctgtta ttttcccctc cagtagcact tttagaggga acaaccgctg tctctggctg
      361 tttatagcta tatgctgttt ttttagcgaa tacaggcgtt tttttaggcg taggacgcat
      421 gccttcgatt tccagtaact cctgatcgat ttcttctaca aaacgagaag gtcggttata
      481 ttgcgtccgt ccatataatg ttctggagaa agcatttgtt aaatacaacg cttcttctgc
      541 tcgcgtgatc cccacatatg cgagacggcg ttcttcctcc agttcgcttt cttccatcaa
      601 tgctcgagat aaaggaaaaa ccccctcttc tagaccaatc aggaatacaa ctggaaattc
      661 taatcctttg gctgcatgca aagtcatcaa tgtcacttgg gaagcatctt cttctaaatt
      721 gtcaatgtcg gaaacaagtg ccagatcatt taagaagacc gttagttttt cttctggtgc
      781 atcagcgtct tcttcatttt gctgttcgaa ttgtttgtcg aattcttgtg tgaccgttaa
      841 aaattcctcc aagttttcga tgcgtgcttg tgcttctaat gtattttgga tttttaagtc
      901 ttctagatat ccgctacgat ccaaaacctc tttcgtcaat tctgtcactg tcaagtatgg
      961 gatcatctga gtgacttctt ggatcatctc tccaaatgca cctaattgct gtcctgcttt
     1021 tccaccgata tttgccagat ccacattttg cgctgcttca agcagtggcc attcatgaag
     1081 agaagcaaaa ctgcgtagtt tttcgataga tcctggtccg atgcctcgtt tgggcgaatt
     1141 aacgatacgc tcaaaactga ttgaatcttg aggatttacc agcacattca aatacgctaa
     1201 aatatctttg atttctttac gatcgtagaa cttgtgccct ccaaccattt tataaggaat
     1261 atttgctttc aacagagttt cttccattac acgagattgt gcgttcgtcc ggtacaagat
     1321 ggcaaagtcg ccgtaattcc gatgattgct tcgaatctct tcttgcatcc ggtctacgat
     1381 aaaacgggtt tcgtctcgtt cattatctgc acggtagtaa gtgatcttgt ttccttcttt
     1441 gttttctgtc cacaagtttt ttggttttcg attgctattg ttttcaatga cttggtttgc
     1501 tgcgctaagg atgtttttcg tggagcgata gttttgttct agcaaaatta ctgcagcatc
     1561 tggataatct ttttcaaaat ccaagatgtt ctgcatatct gcaccgcgcc agccgtaaat
     1621 actttgatct gcatctccca cgacgcacag gttcctaaat ctacctgcca ataaattgac
     1681 tagcgtatac tgtgcgtgat ttgtatcttg gtactcatct acatgaatat aatggaattt
     1741 gttttgataa tacgtgagtg aatcaggatg ttcttcaaat aatcgaatcg tattcatgat
     1801 caggtcgtca aagtccatac attgattata ttgaagctct ttttggtaag cagcataaca
     1861 ttttgccgca atctcttcgt aataactgcc ttgcattttt gcaaaatcct gaggggtctg
     1921 caggctgttt tttgcttgag agattgttcc taatatactt cgaggatcat attttttcgg
     1981 atcaatgttc agttctttta gtatccgttt catcaaggtc agctgttcag aagaatcgat
     2041 gattgtaaag ttccggttat acccgataaa atcaacatct cttcttaaaa tacgcacaca
     2101 catcgagtga aaagtcgata cccagacatc ttcgcctcca gatgcgagaa tcgcattgac
     2161 acgttctttc atttcttttg cagccttatt ggtaaaagta atcgctagga tgttccaagg
     2221 attgacttct ttttcctcaa tcaaataggc aatgcggtgt gtcagcactc tcgtctttcc
     2281 acttcctgca cctgccatta gcagtaatgg tccgtcagtg tgaagaacag cttctttctg
     2341 acgggggttc atcccgttta ataattcagc tttggatgac attaacaaca tcctttcttt
     2401 tatcaatctt gttaattata gcattttctt ggctttcatt aaactagcta gttcaaaatc
     2461 atttgtttct ttttatggat ttttccttgt caattcgttg atttcgcgct aaattaaaag
     2521 aaagcgaggc gtttgaatgg aaaaaaaagg aaaaatcaag aaattagata agactcagat
     2581 atattttatt ctaaaaggaa tatttgttgg actgctgaca ggaatcgtcg ttagcctgtt
     2641 tcgattatct atcgaaaagc taagcgattt agttcgaagt atctatgaaa tgagtcggga
     2701 acagccgatt tacttaattg gcctagctat ttgctgcatc cttgcagcat ttttcgtggg
     2761 ttatctggtc aaaaatgaac cggatatcaa gggaagcggg attccacaag tagtgggaca
     2821 gcttcgcggg gaattatcaa tgaactggtt ctctgtttta tggaaaaagt tcattggcgg
     2881 tgtactttca gttggtgcgg gcctttttct aggtagagaa ggaccttcta tacagcttgg
     2941 cgcatccatt ggacaaggag ctggacaatt gtttcggagt ccatcttcag aagaaaagat
     3001 tttaatttct agcggtgcaa gtagcggact tgcagcagca ttcaatgctc ctatcgcagg
     3061 attactcttt gttttagaag aaatccatca ctccttttct ccacttgttt ggctgacatc
     3121 atttgcttca gcaatcactg ctaacttcgt ctctctttac tttttcggct tgagacctgt
     3181 tttatatcta ggtgaccttc cttctttgcc gttgaaatat tatggttcct tagtgatctt
     3241 aggagtactt ctaggcatct taggttttat ttatcagaat gtcttgctgg ctttgccaaa
     3301 attttacaac cgattgcctt taccagctta ttttcacggg ttcgttcctt tcttgctgat
     3361 tttgccaatt ggttatttat ggcctagtac tctaggcgga ggcaatcaaa tcgttttagc
     3421 ttttggacat gcttccttgc caatcattat gattatcggt ttgtttgttc ttcgattcgt
     3481 tttttccatg atttcttacg gtgggggtct tccagggggg attttccttc cgatattgac
     3541 attgggggct ttaatcggta gcttttatgg aaacattatg gttgatttcg ggatggatcc
     3601 aatctacatc aaggattttg tcgttattgc catggctggt tatttcacag cgattggaaa
     3661 agcaccgctg acagcaatta ttttagtgac agaaatggtt ggctctctta atcatctgat
     3721 gcctttagga ttggctgctc ttgttgctta tatcgtcaat gaccttctcg gcggtaatcc
     3781 aatttacgaa tcattattgg aacgtctact tggaaatact cagaccgcct cactaaaagg
     3841 tgcaaggaca acttttgatt ttccagtaac ggcagagagt tctttagacg gattaatggt
     3901 acgagacttc aattggccta aaaatatgct gctaatctct atacgcagag gaaatcagga
     3961 aatcttgact cacggtgata cgatcatgaa tgtaggagat attttggtta tcttgactga
     4021 tgaagcaaac ttgccaagaa tcaagcagga aatcgaggct aaatcttcta tcacaaattt
     4081 gaagcaatca ttttaacgaa catggaaaaa ctattttaca acaactattc atctacacta
     4141 cttgattcta tatgattcta taaaaaaaga agaggctggg aaaacccagc ctcttctttt
     4201 ttatttctat gcttctgcta ctgcaggagc ttttctcaat aaaccataaa tcacaccaga
     4261 tacgactgct ccgatagcaa taaacaccaa atacatgacc gggtgactca gtaagaatac
     4321 aacgaaaatc ccgccgtgag gagccagtaa tttgattccg aaagctccga ctaatccgcc
     4381 agttaaagca gaaccaataa taaaacttgg aatggcacga atcgggtcag ctgctgcaaa
     4441 tggaatcgat ccttccgtga caaacgatag tcccatcacg atgttcgtca aaccagcatc
     4501 ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta gcaacgaaaa ttgccaatgg
     4561 aggtaccatt cctccagcca taacagaagc catcacgaca cttccgccag ttgcaacagt
     4621 cgtagctaaa gttgctgtac caaacacata agcggctttg ttgattggac cgcctaaatc
     4681 tgccgccatc attccagcta ataatgcacc aagtaaagca gcattcgaac cactcatact
     4741 tgtcaagaaa ccgttcaatg catcattgat tgctttcata gggacattga ccagcaacat
     4801 caagaaacca gtaatcagta agccaaacac tggataaaag aggatcgttt taattccatc
     4861 caatgatttt ggtaagttct tgaataattt acgtaaaaat acgatgacat aaccagctaa
     4921 gaatccacca atcagtgcac ctaagaatcc tgcaccgcct gtattagcta atgcaccagc
     4981 agcaaatcca acgatcaaac cagggcggtc accgatactt gaagcaataa atcctgctaa
     5041 taccggcagc atgaaaccaa atgcagcttg tccgatttgg ttgaaccagc tagcagcttg
     5101 attgtaattc cctagatttg ataattgatc atgtggcacg ccaataaatt ggtcgatcat
     5161 gaaagaaagc gcaatagcga ttcctccacc aataacgaaa ggtaacatgt gtgatacgcc
     5221 gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca tccgcatttc cttcttctga
     5281 actgctttct tttccatcac catggaatac tggtgcagta ccactaaggg ctagattgat
     5341 caactcttct ggtttacgga taccatcact gacaggacga ttgatcagtt cttttccgtc
     5401 aaagcgattc atctctactt ttttgtctgc tgcaacgatt acaccttctg cacgggcgat
     5461 gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg ttcgtttcga ctttgatctc
     5521 cacacccatt tctttcgctt ttttcttcaa agcatcttct gccatgtaag tgtgagcgat
     5581 ccctgttgga caagctgtaa cggcaacaac gaattttctt tctggatgag cagaagcttc
     5641 tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct ttttgctctt cagctgtttg
     5701 gaacaagtct tggacttctt ctggtgtatt tgcttctttt aattttccta cgaaatctgc
     5761 atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa tgtgtgtcgt ttgcgccttc
     5821 tggagctgcg atcatgaaaa acagataagt tggctgtccg tctaaggctt cataatctac
     5881 tcctttttgg ctttttgcga ataaaactgt tgcttcttta actgcactgt ttttcgcgtg
     5941 aggcatggcg attccatcac caagacctgt agaagtttgt gcttcgcggg ctagaattcc
     6001 ttctttatat gtttcgatat cggaaatccg gccggcatca aacatttttt ggaccatttc
     6061 atcgattgcg ccttttttat cagtcgcttg caaatccatg atcatcgctt ctttgaccat
     6121 taaatccttg atattcatga ttatcctcct tgaaactaat caatttttgt gatttcaact
     6181 tctggcagca actcatcaat caattcgcga gctgccaagt catcagaaaa agctgttgcg
     6241 cttccgcaag caactcccca tttaaaggct tcgattggat ctttcgtacg gctgtaagcc
     6301 ccaataaagc cagcaatcat tgaatcccct gcacctactg aatttttcaa tgggcgaact
     6361 aatacattcg ttttataagt tccttgttcc gtgaagagca aagcaccgtc tccagccatt
     6421 gagatcagca cattctgtgc cccttcagct agtaattttt tcccatacgg aatgatttct
     6481 tctacagatt ggaaggttgt gttgtaaagc tcagctaatt cgtgattgtt tggtttgatc
     6541 aacaacggtt tcttaacaag tgcatttttt aggtcatcac ctgttgtatc aatcacgaaa
     6601 tctgcacctt tttctcgaat gatatcaatc agttcttgat aaaatccttt tcgcaggcta
     6661 gctggcgcgc ttcctgacaa tacgacgata tctccttgtc ctactgttcc taatgcttgc
     6721 ttcaactcat cgatttcttc atctgaaata gatgggccta agccattgat ctcagtttca
     6781 gtgtctgatt ttagtttgat attgattctt gtatcttctt ttacggttgt gaacaaagac
     6841 tggatttgtt ctttttcaag ccacttcgaa ataaattcgc cagtgaaccc tcctaaaaac
     6901 cctagtgctg tagattgaga atcgatccgt ttcaaaatac gggaaacatt gatccctttt
     6961 cctccaggta acttgaagtc ttccttcatt cggttcaaat cgcctagttt taacccatct
     7021 acgcgtacga tataatcaat tgatggatta agtgttactg tgtaaatcat tgtattgcct
     7081 ccttaattgt tgtcttttca cggaattcat taacgaattc taatggacaa cgctcggtta
     7141 tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga caactggttg aatttggtat
     7201 gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc taaacgttta acagctgcct
     7261 cttctggatc cggagtcgtg aatccttgtt caagatgggc accgttcatt cccataaatg
     7321 ctttattaaa atgaaaattt tttagttgtt ctatcgcatt agatcctaaa acagcattag
     7381 ttgccagctt tatcgttcca cctaaaataa tggtagcaag ctccaaatca actaaatttg
     7441 ccgcatgttg aacggaattt gtcactactg tgatctgttt tccttgcaga aaaggaatca
     7501 tttctaacgt agtagagcca gcgtctaaat aaatgacatc ttcgtcatta atggtttcag
     7561 ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt gagtgatttt tcactcatat
     7621 tctgctcaaa attcaataat tgctctttct tagctccgcc atgaactctt tctagaaacc
     7681 cactattctc cagctcttgt aaatcacgtc ttatcgttga ttcagaagca ttcaaaag
//
LOCUS       DACEPW010000120         7379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEPW010000120 DACEPW010000000
VERSION     DACEPW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.122"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(341..1240)
                     /locus_tag="IW819_002001"
     CDS             complement(341..1240)
                     /locus_tag="IW819_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1245097.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
     gene            complement(1261..1533)
                     /locus_tag="IW819_002002"
     CDS             complement(1261..1533)
                     /locus_tag="IW819_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ1245098.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            complement(1556..2962)
                     /gene="lacG"
                     /locus_tag="IW819_002003"
     CDS             complement(1556..2962)
                     /gene="lacG"
                     /locus_tag="IW819_002003"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1245099.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMAVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            complement(3104..4810)
                     /locus_tag="IW819_002004"
     CDS             complement(3104..4810)
                     /locus_tag="IW819_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1245100.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            complement(4810..5127)
                     /locus_tag="IW819_002005"
     CDS             complement(4810..5127)
                     /locus_tag="IW819_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1245101.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            complement(5155..6135)
                     /gene="lacD"
                     /locus_tag="IW819_002006"
     CDS             complement(5155..6135)
                     /gene="lacD"
                     /locus_tag="IW819_002006"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1245102.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            complement(6138..7070)
                     /gene="lacC"
                     /locus_tag="IW819_002007"
     CDS             complement(6138..7070)
                     /gene="lacC"
                     /locus_tag="IW819_002007"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ1245103.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            complement(7081..>7379)
                     /locus_tag="IW819_002008"
     CDS             complement(7081..>7379)
                     /locus_tag="IW819_002008"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="catalyzes the interconversion of galactose
                     6-phosphate to tagatose 6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ1245104.1"
                     /translation="AVNKVPGVRSALVRDMTSAIYAKEELNANVIGFGGKITGGLLMN
                     DIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEFLERWDRGDYHD"
BASE COUNT         2272 a         1507 c         1295 g         2305 t
ORIGIN      
        1 cgctatattt aagatttcaa acaactatat taataggaag aaaatttcat ttttcctctt
       61 agttctataa aaagtgtggc taatttgagg caaaatatta aattaaattt gccacaaatt
      121 aatggctcca ctattatttt aaatcaagaa gtagtaaata atcttatttt ttactaccag
      181 actgtcttta gatattagct tttcgtcacc cactacagtt gacatagacc cacaaaaaac
      241 ttaaaaacct tgcgatgtaa gactggcacg gagaagacca atctatcatc acaaggtaag
      301 agtattcaaa tcttattgaa gactcaaata ataacaaatt ttaattaagg atagtaatat
      361 catagatttt tctagaagtt tcttgaggtg aaattttcgt tacctgtctt ttatcttcta
      421 agaaattacc ttcttcaagt gaagtggaga gaccactcca aggttccaaa gcaatgaatg
      481 gacttttatt agttgtagac cacagaatca agttcggaaa atcatcaaaa tctactcgaa
      541 gacctttacc agacttcctt gaccgaagcg ttacgctacg agacttgagc tggtcaagtg
      601 taatggcatc atgggaaaaa agtgagtagt ccaaatcgag aattttttga ttttccaaaa
      661 aaggtgtccg atcttgcaaa tctaataatc ctgtctcagg aaaactcttt ggtatcgagc
      721 aagactcaac ctcactaaat tcaatataat aatcctcata gttttcacct tctactaaag
      781 gactattgaa tgctggatgg gctccaataa agtaaggcat tgttttttcc atttccaagt
      841 tcgttacctg aaattcggtt ctaattgatt taccattcaa ggtataaaca actctcagtt
      901 caaactgata taggtaatta tcaagcatct cagcatttgg tttaatacta aaggtcacgc
      961 tattatcatt gacttcttcc agaataaatt cttcttttcg aacaaaacca tgtctacgaa
     1021 taagacctgt aaaaaagggt ctttcttgag gtctataaat agcccaatca tttcgtaaac
     1081 taccacagat aggaaataga ataggcgcct gtccattcca ataatttgga tcagcttgcc
     1141 agagatactc tatcccatcc ttgtctttaa tcgaagtcag ttgaccacca agtgttttaa
     1201 actgaacagt aagatactcg ttttttaatt cgatagtcac aactaattcc tttcttttta
     1261 ttattggaaa ggcaaataat ttacctcgaa gtgaatagtc tcacgtattt ctacatcgag
     1321 tgattgtaaa aattcccatt caaatgctga taactctaaa tcagaatggc aaatccactc
     1381 ttttccagtt gaaacatacc atttaccaca ttttagcaac ggggtagctt caagctcagg
     1441 aatcatagga caatcttcga aactaacaat atataccttt tcaaagcctg gtctaaataa
     1501 gtgttgtact ttttctaaag tatcggataa caatttttta cattttcaag taatattact
     1561 ctatcacttg agtttctgct actttcttat accaatgagc tgatttctta ggatagcgtt
     1621 cttgcgtgtc aaagtctaca tagaatagtc cataacgttt ttcgtatccg tttgaccatg
     1681 agaagacagc cataagtgac cagatgaagt aacctttaac atttgcacca tccgcaattg
     1741 cgtctgataa aacttccaag tgtttcttca cgtaatcaat acgaccatca tcataaacgg
     1801 tattatctac aaactcatct ttgtatccaa gaccgttttc agtgatgtaa atcttcttgt
     1861 agtttggata atcatttttc actcgcatga tttggtcata caagccttca ggataaataa
     1921 tccagtccca atccgttcta ggcacataat ctggtgcgat tcggcgacca acacctttaa
     1981 tctggtactt agaacttcct ttttcaccct taccattgtg aatgatttca gtctcaccat
     2041 caaaggcttg catccaatca ctcatgtagt agttgatacc aaggaaatcg ttcaaatctt
     2101 tagctgcttc aagtgcttgg aagtcttcat cacgaagatc aagttctcca ccattttcag
     2161 ctaggatgtg gttgacccct tccatcgttt tatctgaata gtgtccaagg taggttgcat
     2221 ccaagataaa tttgttatgg atgatatctt ctaactcagc ggcacgaaca tctgctggat
     2281 tttctggatc ataagggtat ttggttggca acgcatgtac tacaccaatt tcgcctttat
     2341 aacctttatc tttatacaat ttcacagcac gtgcatgaga aaccatcata ttgtggtgtg
     2401 attggaagac tttggcaaga tcatattgaa tgcctggagg gaatttacca accaagtatt
     2461 gaccatctcc aattgggcca atttcattga aagttgtcca gtagtttact tctgggaact
     2521 cttcaaaaca gaaagcggca taatctacaa agtgttctat attttcgcgg tttaagaaat
     2581 ctccatctga gtgaagaact tctggtgtgt caaagtggtg caaagtcacg aatggctcaa
     2641 catgacgttt gtggcactcg gcaaaaagat tatggtagaa ttcaacacct ttttcattta
     2701 cttcgccata gccagttggg aaaatacgag accaggcaat tgaaatacga ataccattaa
     2761 cgccatattt ttcagctaat tctaaatcaa caggatattt atggtaaaaa tcacttgcag
     2821 gctctgctgt gtaccaataa ttgtcttcaa gatacttatc ccatgcaact gggcctttac
     2881 catccgtgtg agtcgcacct tctgcttgat aagcagctgt agcaccacca aaaataaaat
     2941 ctttaggaag tgttttagtc ataactataa atcctttcaa aaaaagcatt tgagacgata
     3001 ttctgagttc cggaaaccaa ggaaatgaaa tgcaatgata tggttaacaa tattataagg
     3061 aattgaaaga ataatctcgt ctcaaataac ttttacaaat ttattaatca aactgttgtt
     3121 gaacaaatgc aagagcacct tgtccatcac gtgtcaattt gatatattga gctccttcag
     3181 tttttgcaag tttaatgccc aatttatccg tttcagcctt catatcatcg aagtttgaag
     3241 cgacctgtgg agccaagata accaagtcaa actctggcaa catttcacgg tgagcaccgt
     3301 aactacccgc tgccgctttg actggaacat tgtattctgc tgctgctttg ttcaaggcat
     3361 tggcaagcaa gccacttgta cctccacctg cacaaagaac aaggacatta gtttcttttg
     3421 tgatattatt gtttgctgct acatcttctt tagcaacgcc tgcttttcca agaacggcat
     3481 ctgctttagc agtattgaag tttgcagcta ctttttcttt aagagcatcg tttgttttac
     3541 cagaacgttc ttcttcaaga atttgttcat cgtatacctt aacaaatggg taataaatga
     3601 tagtatcaac aactaccaag agtccggcta agataaatga tagaacttgg aagttagtac
     3661 cgagtacaat tcccaacgga ccaggagtaa cccatggaag gttggcagag aatgagttca
     3721 tgttcaatgt gtcaacaaag aatttaaaga tccaaacgtt gacaatcgga gcgaaaatga
     3781 acggtacaaa gaaaatcgga ttcaatacga ttggtgcacc aaataagatt ggctcattaa
     3841 ccccaaagaa tgttggaaca actgaggcac gtccgatggc acggttacgt tctgatttac
     3901 aaatccacat gaacaagaat ggaacaatca atgtagctcc tgttccaccc atggtaacaa
     3961 taaacatttg agtaccagaa gtgataactt tgtctgcatg ttgtccagct tggataagat
     4021 gcaagttggc atcgatattg gcataagtga tagcagcgat tgctggttcg acaattgaag
     4081 gaccgtggat accaacaaac cagaagaagg cataagcacc aaagataagt gtaattccaa
     4141 ggtaaccatc tgcagctgag aagagaggag caagaagggt accgattgat tctgcaacgg
     4201 ttacaccaag agttccctta acaatgagtt caaatccata aaggagaaca accgctacag
     4261 tgaatggaat caagtcttta aatacttgag agatatttgg tggaacctct tcaggcatac
     4321 gaatagtaac attatttttc acacagacct tgtaaacatt aactgtcaca aaggctgcaa
     4381 taaatgctgt caaaagacct tttgttcccg tgaaggctgt caagaagcct ccttcttttg
     4441 caggctcagc tgccatcaaa aggaacccta ccatagacgc tagcatcgta gataagaagt
     4501 taatctgatt ggtcgcaggt aggtcacggt tcattgagtc cgtcaaagct ttagcggtag
     4561 taccaccaac aaagaatgct aggataccca tcgaatagct ataaggagtc atcaagaagg
     4621 tctcaatgtc ttttgaccag tggaatcccc aagcatttgg gacataggca ataaggataa
     4681 agatagatga gaaaaggatg actggcatac cagcgataaa tccatcacga atagcacgaa
     4741 gatagatgtt tcgagagatt ttctcaaaga acggtttccc tttctcaata agttcaatga
     4801 gtttatgcat tattttgctc ctcttttata aagttcgatg aggtgatgaa tcacatcttt
     4861 taataagaca gtagtcatta agtggtcctg accatgcatc atggtaacgc tgtatggaag
     4921 ttcctcccca gaagcttctc gagccaacat agctgtctga gaattgtgag cctctgcaat
     4981 acagcttcct gcttctacta caagactatc tgccttagcg aaatcaccat tttcagccgc
     5041 tttaagcgct tctaaaagtt tagagcgagc atctccagca taagcaacaa tttcaaaccc
     5101 taagagagtc atctcttctc tgttcataac aatatcctcc gttttatgtt tttactatac
     5161 tttatcagtc catggactag ctgttttaac aagaactttg ttaagttcat caatattttc
     5221 gaatccagta gtacgcaacc attcacgtgc tgctttttca ccttctttga tgtaaggttc
     5281 tacagatcct gcccaagtag cacgtccaca aagcacacca ttaaacttgg cacctgagtc
     5341 gtgagcaaat tgcaatgttt cttggaacaa tttagcagat acacctgcac ttaggtagat
     5401 gtatggaagg ttagtcgctt cttcttgtgc tttaaagaag tcagcagctt cagctttact
     5461 gtaaactact tcaccatcag caaaaccttc gacatattta acgttaactg gtacttctac
     5521 tttaagaaca tcaatgttaa agcgtggatc agagaaaact ttcatcgctt caatgacttt
     5581 gcgaggtttc acttttgcat attcaacaga acctgcatct gagatttctt catcataagc
     5641 aaggatttcc aagaagaaag gaatatcttc agcgacacat tcagaaccaa cacgttcaat
     5701 ataagcttgt ttttgttggt taagttcatc tgagctgtct acatcatagt aaagcaagaa
     5761 cttaacggca tcagcgcctt gctctttgat acgttttgca gaccaaacat ccaagcagtc
     5821 aggcaaacgt tttgtacttg atgtatcata acctgttttt tcataggcaa gaagaagacc
     5881 tgcttcttta tccaatgctt ttgttgctgg aagtccatat tcagggtcaa gcaacattga
     5941 agaagcgtat tttgtaagtt catcagcaac caaaaccttt agttcttcca tttgagctac
     6001 agttggttct gtatcttgat actgtgccat caaacgtttc aaagcaccac gttggtcaaa
     6061 tgccaaagct gagataacac cgtttttatc tgaaattttt tccaaagatt tgcgtttctg
     6121 ttctgtaagt accattttta tacctctttt actccaattt gtgagtttag tgtttcgtaa
     6181 ttagtcatat tcacatgccc tgtcattgtt tcttgagcat ttaacatacc caatgtcatc
     6241 gcatgtttta ataagtcagc gtcacttttt ttactattta aagctgatgc aataccagct
     6301 accgttgagt cccctgatcc gactggatta acaactggga tatcaggaat atctacctta
     6361 tagaaaacat caccatgttt tgcaaaggca ccgtttcgac ctaatgagac cactatccat
     6421 tcgataccac taaaaagtga ctctttaaga acgtctttga gttcttcaat atcttttgtt
     6481 acttcttttc caagcaactg tgaaagttcc tcattattag gtttgatggc agtaggttta
     6541 gccgacgatt ttagaactgt ttcaagaggt gcacctgaac aatccaaaac gactgctact
     6601 ccttcatccg aagcaagttg gataagtttt tcataataat cgttgggaag tcctgaaggt
     6661 aaacttcctg aaattgtcac aacttctgat tgtttaataa gattgctata gtggtcaaga
     6721 aagccttctg cttcttcatg agaaattgtt ggtccttgtt ccaaaatttc tgtttggtta
     6781 ccctcgtgta agatagcaat acagtttcta gtgtttcctg agattttata aaaagctgga
     6841 ctaacaggag attgttctaa ttcgctttca ataaattcac caattttacc acctaagaaa
     6901 cctgtagcag taactttatc accagattca tacaagactc tagtaacatt taagcctttt
     6961 ccaccagctg tcttactaac atctttcacc ctattcactg tatcaatttt caatgtttct
     7021 agaggataag agatgtctac tgaaggattt agtgtgactg tcagaatcat ttgttatacc
     7081 ttaatcgtgg tagtcaccac ggtcccatct ttcaaggaat tctgtaaaga aatcttcatc
     7141 tgcttgatgt gcattatgtg tttcaacgtg ttcgattttt gcaatcaatt ttttattttc
     7201 ttctgttggt ttgtactcag cttcaatgaa agcttcaatg atgtcattca taagaagacc
     7261 accagtaatc tttccaccga aaccgataac attcgcattt aactcttctt ttgcatatat
     7321 agctgatgtc atatcacgaa ccaacgctga gcgaacacct ggaactttat tgactgcat
//
LOCUS       DACEPW010000121         7326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEPW010000121 DACEPW010000000
VERSION     DACEPW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.123"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            52..948
                     /locus_tag="IW819_002009"
     CDS             52..948
                     /locus_tag="IW819_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1245105.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVKDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDPSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(1003..2373)
                     /locus_tag="IW819_002010"
     CDS             complement(1003..2373)
                     /locus_tag="IW819_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1245106.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQTVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTTIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAMNGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            2611..3357
                     /locus_tag="IW819_002011"
     CDS             2611..3357
                     /locus_tag="IW819_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245107.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            3354..4193
                     /locus_tag="IW819_002012"
     CDS             3354..4193
                     /locus_tag="IW819_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1245108.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            4194..5144
                     /locus_tag="IW819_002013"
     CDS             4194..5144
                     /locus_tag="IW819_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1245109.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKGKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQIKES"
     gene            5284..6036
                     /locus_tag="IW819_002014"
     CDS             5284..6036
                     /locus_tag="IW819_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245110.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFIPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            6055..7206
                     /gene="queG"
                     /locus_tag="IW819_002015"
     CDS             6055..7206
                     /gene="queG"
                     /locus_tag="IW819_002015"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1245111.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         2533 a         1296 c         1523 g         1974 t
ORIGIN      
        1 atatgaagcg tttgataacg ctattataca taaaaattgg tggtgaaaaa aatgtatgat
       61 gaaaatgagt ggggagaaaa tgttcggatg acccgaagaa gacagcaaaa acaggcagct
      121 cgtaagcgga tgatcatgat tggggcagca gttgccgtgt ttttgatcat agcaggagga
      181 gttgcgggtc tatattttca tggaaaaaat tcgtcagaag cgaaaagtac gaccataacg
      241 gaaaccacga caaagaaaac agagacgaaa caaacaagtg agtcatcaac taaaacaaaa
      301 gagacacaaa cttctgaaga aaaaacagca aatggtttgc cagagcaact tcaagcaatc
      361 gtaaaagatt attcgaaaaa gatcgaagaa aagacgcctg cattgattga agaataccag
      421 acagaaattc agggaaatca agagggcata gctggtttat cagcagttgc taaccaaaaa
      481 gcacgtgagc ttcaagcaat ctccgacgaa gggatcagaa aattacgtgc agcgtatcag
      541 gcagcagaaa ataaagacgg tgtagactta gatactttga tcaatcaatt atcggctaat
      601 tatacgaatc atgtagctaa gatttccgat atttatttgc agactagcgc ctctcttcaa
      661 gcagagtcaa caagcacaca agacacaaca tcttctgatt ctgagacggc agagtcggta
      721 gaagcttctg aaatgcaagc tcgaacaaca caagacacaa cagatccctc tgaaactaat
      781 accaacagcg cgacttcaca ggctgcgaca accgtagttt tggaaggaga aggtccaaat
      841 caaatagccg aacgaactgg cgtaccagta gaaacaatct tgtcgctgaa tgggatgagc
      901 atggagaatt atttcctgac tcccggtgag acgctaaaat tgaattgata aaagcaataa
      961 aaaagaacga agcttccact aaggtgaagc ttcgttcttt ttttattttc cggttctttt
     1021 ttcgcgtaca aagtaatgga tcgtcatgta aagcaagaaa acgagtccaa taatcaatga
     1081 gattctcgtt tcaggattga taaacatgaa aatcaatacg atagctaaaa aagcaagtgt
     1141 cacataattc gtataaggag atagtggcat tttaaaagga tgatcatgca ttttatctgc
     1201 gtgcttcttt ttgaagcgaa tctcgctgat caaaatcact acccaaggaa ccataccagg
     1261 caaaatactt gagctgtata cgaaaacgaa aatattttct gactggttga tgaacattgg
     1321 tagaagataa tttaagatca gaccaattaa gattccacct gagattgcta cgacaggata
     1381 aaaaggcgcg ccgtgacgag aaagtttcag aaatttttta gacatctttc cattcatggc
     1441 aagtgtatag accatacgac ttgcgctgaa aatccctgaa ttgcaacctg ataatgccgc
     1501 tgtaatcacg acaaaattaa tcagtcctgc agcaaatgta atgccgactt tagaaaaagt
     1561 ttgtacaaaa ggtgaaccaa tctcactcaa ttgattccac ggatagatac tcacaatgat
     1621 aaaaatagca ccaatataaa aaatcaagat tcgaccgata gtggactgga cagcttcaat
     1681 ggtcgtcttt ttaggattct ctgcttcacc agcagtcatt ccaatcagtt cgattccttg
     1741 ataggaagca acaacgatcg ataatgcaaa gaaaaatcct ttgacgcctc cagtgaagaa
     1801 tccaccattt ttccaaagat ttgaaatccc tactgtctga ccatgattac caaatccaaa
     1861 taagattacc ccaagacctg caatgatcat caaaatgatc gttatgactt tgatgatcga
     1921 aaaccaaaat tctaattcgc caaacgcctt gacagaaata agatttgcca gcataagaaa
     1981 caagacgaca atgatcccgg gaataattgc cggcaaatca ggccaccaat aattcatata
     2041 agatcctaca gcaatcactt cacttattcc tacgacaata tattgaaaaa tattactcca
     2101 agcggtcaag tagccagcca caggatggat gtactcgctc gcgtaatttg caaaagaacc
     2161 agtcgaagga tcaacgtaaa gcatttcgcc taaagcacgc ataatcaaat acaaaaagaa
     2221 ccctgcaatc gcataggctc ccatcactga gggccctgtc cagcgtatgg ttgaggaggc
     2281 acccataaac agaccgacac cgatcgttcc acctagtgcg atcatttgga tatgacgggc
     2341 actaagactt cgagttaact tttcttcttc catacaaaaa ctcctttcgt tgttgagtca
     2401 acaagaaaaa ataatcaaac gtttcaacac atgtttttca ttattaggtt agccttattg
     2461 aactcttgat gtgattctac agaaaatttt cataaaatgc gaggaaaatt cgaaaaaaac
     2521 tttttcatca ttctacttga aaatgctttc cactttagtt aaactatatt taaataaaaa
     2581 gttaggttaa cctaaaaagg agtgtaagat ttggaacgta cggccattgt aattcaagac
     2641 ctttcagccg cttatcaagg gaaaactgtt ttgaaaacta tcaacttgac gataaatcca
     2701 caaaaaatta ccggtattat cggaccaaac ggtgcaggaa aatcaacatt tatgaaatct
     2761 ttgctagagt tgataccagc tactactggt aaagtcacct tttctggaca gccggtaaac
     2821 tcagtccgta aaaaaatagc ctacgtggaa cagcgaagtg aattggatct ttcctttcca
     2881 attgatgtat taggcgttgt acttttagga acatatccat ctttacgaat tggacaaaga
     2941 cctgggaaaa aagaaaaaga acgtgcaaga caagctttga aaaaagtagg gatggaagaa
     3001 tatgccaaaa gacagatcag cgaactatcg ggtggacagc ttcagagagt ttttattgca
     3061 agagctctag cccaaggagc agaatggatc tttttagatg aaccattcgt agggattgat
     3121 gcgttaagtg aacgaaagat ctttgacatc ttgcaggaat tgaagaattc aggaaaaacg
     3181 attttgatcg tccatcatga tcttcataaa gtagaagaat atttcgatga ggttattctt
     3241 ttaaataaac agctgatcgc ttccggtcca gtacaagaag tctttacatc gaagaacctt
     3301 caattggcct atggtgagat catcagacat ttagtaaaag gaggagagaa aaaatgatcc
     3361 aatcctttat tgatggatta atgaattatc aattcctgca atacgctttg gtcacttcca
     3421 tgcttgtagg acttgcttct ggagtcatcg gttcttttat tattttacga gggatgtctt
     3481 tgatgggaga cgctatctct cacgcagttc ttccaggtgt agccatttct tatatgtttg
     3541 gttttagtta catctttgga gccactgctt ttgggatgct aacagcagca gtgattggtt
     3601 tcgtgactca gcggagccga ttgaaaaatg atacagcgat tggaattgtc tttagctcct
     3661 tttttgcatt agggattatc ttgatttcat atgcccagag tgcgacagat ttgtatcata
     3721 ttttatttgg gaatgtctta gctgttaggg aaagcgattt gttgctgaca gcactcgtta
     3781 gtggaatcgt cttgatattt gtctttttct tttataaaga attgaagatc acatcattcg
     3841 atccgacaat ggcaaaagct tatgggttga acacatcgct gatccattat cttttgatgt
     3901 tctttttgac attagtggct gtagtcagtt tacagacagt aggaacaatc ttggtgattg
     3961 ccatgttgat cacaccagcc gccacggctt acttgctaac gaaccattta ctgaaaatga
     4021 tcattacagc tgcaggaatc ggtatgctaa gtgcagttgt cggtgtgttt ttcagttata
     4081 gttacaattg gccatcagga gctacgatcg tgttagcatg taccgcattt tttatccttg
     4141 cttttttatt ttctccaaca aaaggaattt tatttcagaa aggaagcaga tagatgaaaa
     4201 caaaaaaatc actcttttta atcttggcag ttagtttctt ggtattagcc ggttgcggaa
     4261 agcaggcttc tgaccaagcg gatgaaggaa gtaaaggaaa gttgtcagtc gtggctacca
     4321 attcgatctt ggcggacatg gcaaaagagg tcggaacaga tcaaatagat atccacagta
     4381 tcgtaccagt cggaacagat ccgcatgaat atgaagtact accagaagac atcaaaaagg
     4441 caagcgatgc ggatgttatt ttatacaacg gtttgaatct tgaaacaggt aacagctggt
     4501 tcgataactt gatggaaacg gctaaaaaag aagaaggaaa agattatttt gctgttagca
     4561 aaaatgtaga acctctttat ttaactagcg gtgaagaaca tacaaaagca gatccccatg
     4621 cttggctaga cctatctaac ggaataaaat atgtggagga aatcgcacgt atattctctg
     4681 aaaaagatgc agaaaatgcg acactatata aaaaaaatgc agaagcatat gtggaaaaac
     4741 taaaagaatt agatacacaa gcgaaggaaa gttttgcttc tatcgaagag aacaaaaaat
     4801 tattagtaac aagtgaaggg gcattcaagt atttttcacg agcatatgat ttgccagcag
     4861 cttatatatg ggagatcaat acagaaagtc aaggtacgcc tgatcaaatg aaagcgatca
     4921 ttgatcagat aagagcaaaa gaagtaccgg ttttattcgt ggaaaccagt gtagactcaa
     4981 gaagcatgga acgggtagca aaagaaacgg gtttgaaaat ctatgataaa ctgttcactg
     5041 attccatagc aaaagaagga gaacaaggag attcttacta tcagatgatg aaatggaata
     5101 ttgaaacgat ccatgaaggt ttgtcacaaa taaaagaaag ctgaaaagtg caaggtctag
     5161 cttgaggagc aggccttgca ttttttataa gagatatgaa aaaaattatg ctgtttttcg
     5221 gcgtacagat accttttttg aagaaattcc gttatagtta gagtaaggaa ggaaggtgtg
     5281 gctatgaatt ttacacgtaa agataaatat acgattaaag aagaagtctt tcgattgatc
     5341 agccagcagg aaaataccga caaggatcaa gaccttcaat ttcttgaagc agaattagct
     5401 aaattattca tagcaaatca aaatgagaca acagaaccag atgaagagct gtacataggc
     5461 aaatggttgt ttgatgaatt tcagccttta gatgaaaata ccggtatcgg ttattttgtg
     5521 ttcctttctc cagtcggctt catacctggc gtattagtta aacaaaacga cgaaataacc
     5581 gtcaaagcat ggcctgcatt attttcagtc atcaaagcag aaggagtaat caaaaagatc
     5641 cagtcggcct attataaaag tcaatttatt gccaacagca aattaatgaa tatgttttat
     5701 aatcgtctag tagaaattgc caggcctggg aaaatcccgc aaacattcac gtacacacag
     5761 caaaaaaatc tctatgaaga agtattgtat tcccgcgatc tgtctgagtt ttcgccatgg
     5821 gaagaggtaa ggctccatga actgttaagt acaaaaccag tttcattgaa tttttttaat
     5881 gcattagata acaaaagaaa attaatttca acaaattatt gggaagtatt tgctgcaaga
     5941 aaaaaatgga tggaagaaaa gaagattgcc gatcagcaga tccagtttgt ccagctgaaa
     6001 aaagaaaaac tatattatgg gttatacagc cgttaacagc aagcgaggaa gaccatgaca
     6061 ttaaaagaag aaatcataca agaaagcaaa cgattaggaa ttgacaaaat cgggttcacc
     6121 acggcggaac cttttgattc attaaaagaa tcgttggaag aacaaaaagc agctggccat
     6181 acttctggct ttgaacatcc aaatatcgat gagcgtcttt atcctgaaat gacatttgat
     6241 cagccgaaat cgatcatttc cattgcactt gcgtatccta ctaggatcca tgaagaagtg
     6301 cctagggatg aaaaacgcgg acagtttgct cgtgcttctt gggggatcga ttaccatgat
     6361 atactcagag accgtttgaa ccgattgatt gaattcatcc aaacaagagc agaaaaatgg
     6421 caaaaagaag aggaatggcg tctagctccg caagtagata ctggagaact gatcgatgtc
     6481 gctgtagcac agcgagctgg tttaggattt attggacgaa acggattgtt gatcactgaa
     6541 gagttcggtt catttgtcta tcttggtgag atcgttacta atattgtatt tgagcctgat
     6601 aaaccaggag aatttggttg cggtgactgt atgcgctgcg tgactgcttg tccgacaaaa
     6661 gcattgcttg gtgacggaag aatgaatgcc cagcgatgtt tgtcttatca aactcagaca
     6721 aaagggatga tgccggaaga atatcgtaaa aaaatgcgaa atgtcattta tggatgtgat
     6781 atctgccagt tagtctgccc ttacaatcaa ggaaaagatt ttcattttca tgaagagatg
     6841 gaaccaaaag tagacgaagt atatccaaaa ttaaagccaa tgctttcgat gtctaataaa
     6901 gacttcaaac agcaatttgg gcatttagct ggttcatggc gtgggaagaa acctttgcag
     6961 agaaacgcat tgattgcttt agctaatcta ggtgatcgaa cggcattgcc tgagatttca
     7021 gaatgtttaa cagatgtccg ccctgtcatc agaggaacag cagcttgggc tattggacgt
     7081 ctaggaacaa aagaaccgga aaaatggctt gatgtattag atgaaatatt agctaaagaa
     7141 acagaagaag tcgttataaa cgaattggaa catgcccaga aattattgcg gagaaagtta
     7201 aaataaaaaa atagttaaca ttattcaaag tttttgtcca gtaattgttg taacaattac
     7261 tggttttttt gtatttttta cattttttta agagaatttg ataaaaatca aaaatttttt
     7321 tgcttt
//
LOCUS       DACEPW010000122         7215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEPW010000122 DACEPW010000000
VERSION     DACEPW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.124"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..304)
                     /locus_tag="IW819_002016"
     CDS             complement(<1..304)
                     /locus_tag="IW819_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1245112.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTK"
     gene            complement(488..1381)
                     /locus_tag="IW819_002017"
     CDS             complement(488..1381)
                     /locus_tag="IW819_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1245113.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKNNVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQLRKNNR"
     gene            complement(1455..3152)
                     /locus_tag="IW819_002018"
                     /pseudo
     CDS             complement(1455..3152)
                     /locus_tag="IW819_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(3145..4149)
                     /locus_tag="IW819_002019"
     CDS             complement(3145..4149)
                     /locus_tag="IW819_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1245114.1"
                     /translation="MTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGL
                     GNGIARIKAPKKNQDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTE
                     ELQEIHIYPKNVGYLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGEKYYLDMAP
                     ANDLKNQWIKPADNDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEM
                     DEASKRIEVIIPEFWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVK
                     ETTDKPKEPSGPSNPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE
                     "
     gene            complement(4146..4457)
                     /locus_tag="IW819_002020"
     CDS             complement(4146..4457)
                     /locus_tag="IW819_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245115.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKLLK
                     T"
     gene            complement(4457..6961)
                     /locus_tag="IW819_002021"
     CDS             complement(4457..6961)
                     /locus_tag="IW819_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245116.1"
                     /translation="MKNIADLEEDGVIPRYYNANNKNDIVTALEDISSTFKRTIQQAT
                     LYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTT
                     YVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFYTVNNMAYIQNLEDYENDI
                     TTGRMYFPSPSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKT
                     TTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQCQIKKYAANEDEM
                     LGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMR
                     NEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGR
                     LTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEP
                     DENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRV
                     RKLDHESHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVE
                     KYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDENA
                     PLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEII
                     EGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDR
                     VVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPAT
                     GGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
BASE COUNT         1840 a         1646 c         1248 g         2481 t
ORIGIN      
        1 gttttgtcac aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat
       61 tcctgctgga gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat
      121 aatagccatc caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt
      181 caaagtctga gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct
      241 caagttcctc catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg
      301 ccataaaaaa tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa
      361 acaagttggc ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg
      421 cgatttaatc gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat
      481 tggtctatta tctgttattt ttccttagtt gtttttgtct tttaaatttt cgataacacc
      541 aataaccgaa aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa
      601 gatggtattt ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgcc
      661 ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga
      721 ctagatcctg cttttctctg attgctaatg tatttgtttt tgttggaagg acaacaattt
      781 tctcaacgac ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt
      841 gctccatttc ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa
      901 tcacggaatg tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc
      961 ctctatctaa caaaagatca ttcgtttcat caaataaagg caagctaaca ttgtttttcg
     1021 gaatatagat ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat
     1081 ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat
     1141 gagcaagcgt ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt
     1201 tatcgcgttc aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga
     1261 gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca
     1321 taaataattg taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca
     1381 ttttcttcac cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag
     1441 cttatttttg ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct
     1501 ccaatcatca aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca
     1561 ggaagtgacc cgcgtttcat attcggtact ctttatggcc ctggattttc tactcctacg
     1621 atctgaattt cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca
     1681 cctgttggaa gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa
     1741 tctaaccctt gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg
     1801 cttgttgccc actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct
     1861 ttataaaata ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta
     1921 agcaattcac tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc
     1981 tctggcggct ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc
     2041 gctgtgttgt tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg
     2101 atcgttaaat ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact
     2161 ccattttctt ctgtcaaaat cgtcacgaca aacccgttgt ttactacttc tactatataa
     2221 tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa
     2281 gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt
     2341 tcaaggacag aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt
     2401 cctctttcta gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt
     2461 acttgtctaa ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt
     2521 ttcggataca aatgcacatc tgtattgtat gtgtctttct atgtagaacc atcccacaca
     2581 ccatccacca tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg
     2641 gaaatatgag ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt
     2701 gcttcttccg tcacttggac ggtagaaaat accgcttctc cagcttcatt cgttgtttgt
     2761 ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc
     2821 cattcaatcg cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct
     2881 cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata
     2941 ttttcttcac caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat
     3001 gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg
     3061 gctttgacta gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca
     3121 ctcattcccg tgacaatcat ttttttattc atttttttct ccccgttctt tttttattaa
     3181 taaaacagca cctaacaata aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt
     3241 cgtttgcggg aatacggacc attttcctga ttgacctgga ttgcttggac cactcggctc
     3301 tttcggtttg tccgttgttt cttttacggt ctcgttataa acacgtggtg ttgcggattc
     3361 ataggcagag actggaactt tcccggattc taattcagcc atctcctgtc cgttaactac
     3421 tacatattgt ggttggcctt ttgcatccac ccaaaattca gggatgatga cctcgattct
     3481 cttacttgct tcatccatct cgtaaccttc cacacctttt aattcttcaa aataataggt
     3541 gccagaaggc aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg aaatgaattt
     3601 gcttacattt ttgtcattca aaggatcatt gtctgctggt ttgatccatt gatttttcaa
     3661 gtcattagca ggagccatat ctaaataata tttctcccct tctatcatct gatatagagc
     3721 aaaaacagca ccttctaatg gttttccttt ttcagttgtt cctctttgac gtccatactt
     3781 gaagaagtat ggatctctta gataaccgac atttttagga tagatatgaa tctcttgcag
     3841 ttcctcggtt gattctgtcg ggtgataaat tggcaaaata gcagcaattg ggatagctgt
     3901 ctgatcgata tcaacatcca atccaacttc tggatcaatc gattcttcga aaatcaaata
     3961 aactgcatcc cgatcttggt ttttcttagg tgctttgata cgtgcaatcc cgtttcctaa
     4021 tccgccaacc gtatctttcc cagttgttac cgaagtccct atttggacaa gcctttcttc
     4081 tgtgattagc tgacggatat tgtttctgtt cgtttcagcg atttgctgga cgaattcttt
     4141 aggagtcatg ttttcagcag ctttttttca tgataataat cggtcatgtc gtaaatggca
     4201 aatgtcgctc catttagtgg caccgtctgt gaaatgatat ctgctgtttc ccctgcttcc
     4261 tcagagatag ccaagccatc attttcatgg tattcaaatt gatcgttata atcgatatct
     4321 cgaatcatcc gtttatgcaa cacgaattcc acgacatctt cccctgattc ttctgccgac
     4381 atcgcctgtg gaagaaggac tgctgcaagc ggtagtaaaa caagtaattg aagcatcaaa
     4441 cacaacagct ttttcattac cttgctccct cctttttaga ttgtctgaca aacaaaccat
     4501 aggctgctcc tgcaagcgcc atgaagaatg cagcaagcag gatatagaat aatcgtccgg
     4561 atcctcctgt agcaggcaac ggattttcag gatcgttccc gacactaagc tgtaatctga
     4621 ttcgatttgt ctccaaaagt tcatattcta actcttgctg tagatcaggc gtctctgctt
     4681 cttcgtttgg ataatacgaa atctcaactt ttagtgtacc cgcttctttt cctggaacta
     4741 cccgatcatt ttctatgtca ggttcttctc gataactaat ttctaaaata aagtaacctg
     4801 gaagttttct atagctgtca ggtgacttca cttccttgat ccgatagacc ccttccaaca
     4861 agtctgaagc attgaatagg gaagaatcgt ttggatctgg tgccaagcct ccctcgatga
     4921 tttcatactc tcctgcacta ttttgtttct caattgaaaa ttctgcgcct tcaagacgtc
     4981 ggctattcat actgtcaatt ttcaaaagtt ccaaatcgat tggcgctagt tcatcttcta
     5041 aggttacttg tagtactcca tcgatgattt catagccttc cggcaaggga gcattttcgt
     5101 cttcgatgac cgtatcttca atgataaact taccttcttc attcagtgca aactggaaca
     5161 cttgatcaga tagccgatac ccatttggtg cgacagattc cgagacctca tattcaccaa
     5221 tagacagatg cagaggttgg tcgtaattta aatcgacata cttttctact cctacggcac
     5281 gagaatagaa gtatccgatc ccaggcaaac gtttcgattc ttctggttct aacggattac
     5341 cagtgtatgc tcgatacagc gggaaggaag aatcccccac tttcctgata tcgaaacctg
     5401 caccatctaa tgtatggctt tcatggtcta attttctaac tcggatctct cgataatcaa
     5461 acgtattgta agcagtgaat gctacccata aacgatcctc ttcatctgtt gtatacgtga
     5521 gatattgcca attttcttcg cctgtttgct tgagctggat agtccctata gcattatcgt
     5581 aaagtcgcaa gatccatgtg tcattttcgt caggttcttt gaaaccttct gggttctggc
     5641 tctcaatgat tttgtattct gcatagttgg tagtttcttc actatcctta taaacaaatg
     5701 aatctggctc tccgattgga tgaataactt tctcaccatc cacgattaca tatttcccgt
     5761 cttcatctac tgaatagaac gtgagtctcc ctttctcttc ctctacttcg ttcacaccag
     5821 aaagcccttt cgctactgga tatccgattt ccagtccgtt accatctgtt tcataaagtt
     5881 caaactctgc gccattgatt ggcatattac tgccttcaat tagtttattg atattcatgt
     5941 ggattggctt gaattcattt cgcatttcca gtttgacata caactcatca tactctgtgc
     6001 cttctatgtt gggctcttgt acaacagaaa gcttgtgata ttctccttca taataggaat
     6061 tcggatcttt accttccggt ccgccgactc tcgaaccaga tgtatattca tctacaagct
     6121 cgtagtgacc taacatctca tcttcgttgg ctgcatattt tttgatttgg cattgatatg
     6181 tctcatctac taactgataa cgtggtggtc cagcgatctc tctgatccaa aatatgtcgc
     6241 tgactgctcc agtttcatct cctaaaatcg gcatcttcgt ttcaaagatc gctattccgt
     6301 ccgtggtcgt tttacttgtt tcgattggtt cggcttcccc actctctgga ttttggtcat
     6361 ataatgcaaa ttcgactcca gccaacggct cgccattttg acccttcttg ctgacttgga
     6421 aattacggcc tttatgttcg taacgaatgg aaggagatgg gaaatacatc cgtccagtag
     6481 tgatatcatt ctcataatcc tccaggttct gaatataggc catattgttc actgtgtaaa
     6541 aatttccgtc ttgagctccc gcatcaagtt ttacttggta cttgatcctg accatttccc
     6601 cttcacctaa agaaatcgga gaaaatttat aagcttggtt ttcccgtccg acatacgttg
     6661 tcggagtcgc cactacttct ggtggggctt gtccatgacg ttcttcatcc catatttctg
     6721 gctcttggta cttcatgccg ttttctcctg gatctagatg gtaaaactga atatctgccg
     6781 atctgccagc tccaaatggt gagacattaa aaccagtttc atcataaagt gtggcttgct
     6841 ggatcgttct cttgaaggta gaagagatat cctccaaggc tgtaacaatg tcattcttgt
     6901 tgttcgcatt gtaatatcgc ggtattacgc catcttcttc taaatcagca atatttttca
     6961 atacatttcg tccaatagca tctagtcgtt gtctcccgct aacgtttcca tcgattccta
     7021 aaccaatgga gaagatactt ggcgggttct cttgatactt gtttttcagc caatgatgat
     7081 aagcaactgt gttgatcgca gatgactttt cttgcgaagt aggactgcca ttcgtattgt
     7141 tattagaaga accatcacca acccagtgag tcgctttatt atctccttct tttacctctc
     7201 cataacgata gtgta
//
LOCUS       DACEPW010000123         7214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEPW010000123 DACEPW010000000
VERSION     DACEPW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.125"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            99..746
                     /locus_tag="IW819_002022"
     CDS             99..746
                     /locus_tag="IW819_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1245117.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IW819_002023"
     CDS             1139..3784
                     /locus_tag="IW819_002023"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1245118.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IW819_002024"
     CDS             4094..5407
                     /locus_tag="IW819_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1245119.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IW819_002025"
     CDS             5397..6050
                     /locus_tag="IW819_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1245120.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /locus_tag="IW819_002026"
     CDS             6105..6797
                     /locus_tag="IW819_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ1245121.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="IW819_002027"
     CDS             6947..7147
                     /locus_tag="IW819_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1245122.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2386 a         1220 c         1585 g         2023 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaa
//
LOCUS       DACEPW010000124         7180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEPW010000124 DACEPW010000000
VERSION     DACEPW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.126"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..993)
                     /locus_tag="IW819_002028"
     CDS             complement(<1..993)
                     /locus_tag="IW819_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1245123.1"
                     /translation="MDLNKRQLQILVVLRNKNHWITSEELAEALGTNKKTIQTEIKNM
                     LAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRASTIVTYLLFQ
                     KGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLKIIAEENSKRI
                     FLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSGEDFIRFSRYL
                     VLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIENRLLELNYLM
                     INDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTRMDAGHNT"
     gene            complement(1073..2569)
                     /locus_tag="IW819_002029"
     CDS             complement(1073..2569)
                     /locus_tag="IW819_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1245124.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGGQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(2763..4385)
                     /locus_tag="IW819_002030"
     CDS             complement(2763..4385)
                     /locus_tag="IW819_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1245125.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(4834..6147)
                     /locus_tag="IW819_002031"
     CDS             complement(4834..6147)
                     /locus_tag="IW819_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1245126.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(6392..6616)
                     /locus_tag="IW819_002032"
     CDS             complement(6392..6616)
                     /locus_tag="IW819_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245127.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(6636..7004)
                     /locus_tag="IW819_002033"
     CDS             complement(6636..7004)
                     /locus_tag="IW819_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1245128.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
BASE COUNT         2173 a         1518 c         1130 g         2359 t
ORIGIN      
        1 ggtattatgt ccggcatcca tcctcgtttt catttgtttg atatgggtta gcaaaaattc
       61 tgtttcatgg aacaataacg aaacaggaag atctaaaaaa cgaataatcg tttgctcaaa
      121 tgctcttaga ttcttctcta tttctttgtt tttttcatca ttaatcatca aataatttaa
      181 ctctaatagt cgattttcaa ttgcttgttt ttcttgattg gaaaaagaat agggtagttc
      241 tgatgataac ttttttgtga gggtctctac catcggaata aataatgtat acctgtttgt
      301 tttttctaaa agttcttctc ttcgaagaat ggtcaaaacg agataccgag aaaatcgtat
      361 gaagtcctct cctgaaacaa tataattata tgatataaga acctctttta atgtcttttg
      421 gacagtcggc atgatattgg aaaaaattgt actgatttgt tgaggcaacc ttgcgtgttg
      481 gatcaccgtt tctgttccaa caagagataa gaaaattctt tttgaatttt cttcagcaat
      541 gatttttagt ccttttgtcg acgaaacttc cagttctaat ttaggattgc gctcgatcca
      601 acgcaaaatc gtttttattt gtaatgatac ggctgttttt gaaagaaaga aaatgtctgc
      661 tatcttttgc atactgacat accctttttg aaacaataag tacgtcacaa tagttgaagc
      721 tcggaaatcc atgctggaat agattttatg tttcgtcacc tcttgtatga tttgctgttt
      781 agtttcagat tcaattgatt ctaaaaaata acctgaacgt ttatttgact ggataatgat
      841 tttcttctcg tagatagcaa gcatattttt gatttcagtt tggatcgttt tcttgttggt
      901 acctaaagct tcagctaatt cttcgcttgt tatccaatga ttcttatttc ttaatactac
      961 aagaatttgg agttgtcgtt tatttaagtc catttgctta cacctcctct ttgttataaa
     1021 catacaggaa aaacctctca aatgacaata gtcacttgca gaggtttaaa aatcatttga
     1081 agaaagatct atattcaagg tttttaccat tagactggat aatttgttta taatattcaa
     1141 aactatcttt tttcaatctc ctaaagcttc cgctcccatc attttgtcta tccacataaa
     1201 taaagccata tcgcttcctc atttcccctg ttcctgccga tacgatatcg attggtcccc
     1261 accataagta accaatgatt tggcaaccat cttttattgc ctcagcaatt tcctgaagat
     1321 gtatttttag atactcacaa cgttttggat cgtggattcg attcaattca tctggtgctt
     1381 catctagtcc aatgccattt tcaactatga ataatggtaa ttcataacgg tctgtcaatt
     1441 cattcatgac ataacgaaac cctagaggat caattggcca actccaaggt tcgggagatt
     1501 tttccttcaa aaagggatta tcgaaaccat ttgctccgcc agtatctacg cgcatttctg
     1561 cttctttttt atagacagcg ctacgataat aactaaatgc aatataatct gccgtgttag
     1621 cttgcaagca ctgttcgtct ccatctaaca ttactggtgc acagtcttct tccttccatg
     1681 tcctgtaaat ataagctgga taatgccctc ggcacatgac atcggagaaa aaccatgaat
     1741 tgcgtttaaa ttgcagtgaa ccaaatacat tctctggatc acagtcatat gcataagtcg
     1801 ctaatgcaga aaagctacac ataataccca tcttagcatt tggatcgatt ttctttaata
     1861 attgagtagc acgggcgttc gctagaaaca tatgatgaca ggcttgataa atcacttttt
     1921 ttgttagtgt agcaatgggt tcaagtttat ctctagcagg gaatgtaacc aatccagcag
     1981 ctgcataagg catacgaaaa gcattattga tctcattaaa ggtcatccaa tatttcactt
     2041 tcccttggta gcgttcaaag acggtctgcg cataaaattc aaaaaagtcg attaattttc
     2101 gattttgccc ccccccatac tctcctacca atgctaaggg ggtttcataa tgactaagag
     2161 taattactgg ctcgatatca tatcgattta atgtattgat tagtctgtca taaaatacga
     2221 gaccagcctc atttggcttc tgttcgtctc ctcttggaaa tattcgtgcc catgagatgc
     2281 ttgttcgaaa agcttttaaa cctaactcct tcagtaaacg aatatcctct tcaaatcgat
     2341 gataaaaatc aatcgcttca tgagaaagat aatggagtga ttcgtctaaa gcaatctccc
     2401 aaatcttttt ttcttcattc cactgaatac taggatgctg gttcatgatc ccgataagcg
     2461 tatctgtcac attaggtaat ttgccgtctt ccaaccatgc accttcacat tgattagcag
     2521 ctactgctcc gccccataaa aaattttctg gaaatatttc ctgatccata actatctctc
     2581 ctcgctatca acaatttctt acttgaagta tagctcgatt catcaaaaca agatattcta
     2641 actatttcgg cagaagagaa gaaaaaaatt gaatgctact cttcattttt aatatttata
     2701 tataacaaat acataagacg atccccgaaa gaaaacactc gttaaccatc taagcaaacg
     2761 atttatttta cttccaacac aaatgcttca taaggtcgta aatcaacttt gccgtctggt
     2821 aactgtttca gaggataatt tgacacaata acccttaaag cttcacgtgg aacagtgaac
     2881 gtctgctttt cactggacat attacaaata acgagccatt gctggtcttc atatatgcga
     2941 gaataggcaa agacatgctc aggcgtcttt tccactaatt catagtctcc ccagacgatg
     3001 atgtcatgtt ttctacgcaa atcgatcaat ttcttgtaca tataaaaaac agaatcttga
     3061 tctgctaacg cttcttctgc attgatttct ttataatttg gattgactcg aagccatgga
     3121 attccagtag taaacccagc atgttcacgg gcgttccatt gcataggcgt cctagcattg
     3181 tctcttcctt tcgcattgat tgattccaag atttcctctt ttgtaaatcc agaagaaatc
     3241 ctttcgttat acatattgat tgtctcaata tcttttgctt ccgcgatatc atcgattggg
     3301 caattcgtca tgcctaactc ttctccttga tagatataag gggtgccttt catcatatgg
     3361 agcaagatag caaacatctt ggcactttct tttcgataca catcgtcatt tccccaacga
     3421 gaaacaattc gcggaagatc gtgattattc caaaataaac tattccatcc ttcatcacct
     3481 aaagatgttt gccatttgga taatactttt ttcagttcag agatactcaa gggtttcaaa
     3541 tcccatttgt ctttgccttc ttgctgatct aatcctatgt gttcgaattg gaagaccatg
     3601 gataattcat ttcttttagg attggaataa agtttagcga tttcggtagt tgctccccat
     3661 gtttcgccaa cagtcatcag atttttgtca ccaaaagttg cttgattcat ttcctgaaga
     3721 tactcatgca gtttaggtcc attacttgtg atttctttat ccggttcttt gccaatcata
     3781 tcgatcacgt ccatacgaaa gcccccaata cctttatcga tccagaaatt catcatttga
     3841 taaatctctt ggcgaacctg tttatttttc caattcaaat caggttgacg cttgctatac
     3901 aagtgcagaa aatattgccc cgttttttca tccaactgcc aagcagaacc actgaatatt
     3961 gagcgcaatc tattaggctc ttttccattg accggatcac gccaaatata atagtcccga
     4021 taaggatttt ctttattttt acgggcctca atgaaccacg gatgttcatc agaagtatga
     4081 ttcaccacca gatccatgat gatacggata tttcttttct ttgcttcttc aatcagttct
     4141 tccatttctt ccatcgtacc gaattctgcc atgattgctt gataattact gatatcatac
     4201 ccattgtcat cattcggaga ctcgtatact ggagaaagcc aaattgcatc aatacctaaa
     4261 tacgccagat aatccaacct ggaaataatc ccccgcaaat ctccaatacc atcttgattt
     4321 gaatcttgaa aacttcttgg atagatttga taaacaactg aatggtgcca ccatttttgt
     4381 tccatgattt tcctcctata aatggatgtt tctatttttt gcttaatcat ttattttagt
     4441 ctctctcact aaaaattgta acgctttcaa atagctattg caccttgtta tcggtaacag
     4501 aataaaacaa aaaaacagct gaggaaattc cctcaactgt tttttgaaag gattgattta
     4561 tgcagggaag aatgctgatt tcacaacttc tttataaacg gtgttcactc agctgtccct
     4621 tggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttcgtttt tttctctaat
     4681 actcaggcca gaacactgat ttcacaacct ctttataaac ggtgttcact cagctgtccc
     4741 tcggtattag ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa
     4801 tactcaggcc agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa
     4861 tcccacaaat acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc
     4921 tgctaacatg gtaattggat agaagataaa acctaatgca atcccatctg aaatactata
     4981 agtcaaaggc atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca
     5041 ttcgatttgt cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt
     5101 tacttgtgaa gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat
     5161 ccctgtagta atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc
     5221 tacaaatgca ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc
     5281 agaagctaaa gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt
     5341 tgctaatcca actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac
     5401 tacgacccac atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt
     5461 tggtttcaaa cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac
     5521 cagacctaaa atcgttgtag ctgccatccc gatgaagatg ccacctggta cacgccgaac
     5581 aagcaagata gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag
     5641 tgatcctaat cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc
     5701 aaggaaagcg ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat
     5761 cgcatcaatg atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc
     5821 aacgaatacg ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata
     5881 agcaaaaaaa gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac
     5941 ccccattaaa acacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc
     6001 atccattcca gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat
     6061 aaatgttgta aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc
     6121 gaagtaggaa agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata
     6181 aagtccgtta aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa
     6241 aacataagta aacaaaaaat tcggatttta agcaaaatcc gaacgttcca tctgattaaa
     6301 tccctttatc tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt
     6361 taataaagtt tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc
     6421 gtttctgttc ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc
     6481 caaaacctgt atcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat
     6541 tctttgctcg aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc
     6601 ttcccattag atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct
     6661 ataacaaaat aattgccttc agggtcagaa aaattgaaag tcaagatgcc attgttatct
     6721 gtgatttctc ctggttcttc catgttctcg tagatagttt caaattcatc cgtaaaaaat
     6781 actaaagaag gcggtggtcc taatacctct ggtgaaaact tctgcacaaa tacttttggg
     6841 aatacgccta attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc
     6901 aattcgattg tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc
     6961 tctacgtcat tgacataaag catcacacgt gttttttgta tcatcatttg acccttcttt
     7021 ctatttgatt ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac
     7081 cttgtgtttg atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca
     7141 caaacttttt ttacaaaaaa aaaaacggca atgaatgccg
//
LOCUS       DACEPW010000125         7163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEPW010000125 DACEPW010000000
VERSION     DACEPW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.127"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..628)
                     /locus_tag="IW819_002034"
     CDS             complement(5..628)
                     /locus_tag="IW819_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245129.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(787..2184)
                     /locus_tag="IW819_002035"
     CDS             complement(787..2184)
                     /locus_tag="IW819_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1245130.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2216..3187)
                     /locus_tag="IW819_002036"
     CDS             complement(2216..3187)
                     /locus_tag="IW819_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1245131.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3561..3986
                     /locus_tag="IW819_002037"
     CDS             3561..3986
                     /locus_tag="IW819_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245132.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            complement(4474..5175)
                     /locus_tag="IW819_002038"
     CDS             complement(4474..5175)
                     /locus_tag="IW819_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1245133.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIHQIQEKLAELEGKPAG
                     KYFTDNQHAKGQGLG"
     gene            complement(5191..5631)
                     /locus_tag="IW819_002039"
     CDS             complement(5191..5631)
                     /locus_tag="IW819_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1245134.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5631..6206)
                     /locus_tag="IW819_002040"
     CDS             complement(5631..6206)
                     /locus_tag="IW819_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1245135.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         2035 a         1460 c         1122 g         2546 t
ORIGIN      
        1 tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg
       61 cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc
      121 ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt
      181 tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga
      241 tcacttttcc atttttcaaa cgggctactt tgtcgccatc atgagtcaaa agcaaaacgg
      301 tcaaatgctt gtctttaaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga
      361 tattaataaa ttgattttct tcttttcctt ttggcaaata gatgtccaat tcgccttttt
      421 cgtaattggc agttatttta tactccgccc accaataagt tgagaattct tttttgaaat
      481 tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg
      541 tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa
      601 agagaagaaa cggtcttttt ttcctcatgc tttcctccac tcctatttaa ttttgactat
      661 tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat
      721 tgaacttctt tttcacaaaa tggtaagaat ggagaatttc acgcatttgc tctcaactat
      781 tctttcttaa gcctcaaatc cgtttttatc tgatacgtct ttgaaccagt gacccgattt
      841 tttgattgtt ctttttccat cttgtgctaa gtctactgaa ataaagccat atcgattctt
      901 atatgcattg gtccatgacc agttatccat acatgtccac atatgatagc ctaaacaatt
      961 ggcaccttct tgaattgctt gatggacata tttcagatga gattggacaa attcgatccg
     1021 ataatcatcg tggatcatgc catcttcttt gataaatcgt gcttcgtttt ctacacccat
     1081 accgttttca gaaataaagc agtcgatatt tccataatga tctcgtacat tgatcaaaat
     1141 atcatagatt ccttgttcat agatctccca tccgcgataa ggattcattt tacgaccagg
     1201 catttcataa ttgtcgaaat aatcctcagg cattggaccg tttttagtat cgattggtgt
     1261 ttcttttgct tttacccggc gtggctggta ataattgata cctaaaagat ctactgtatt
     1321 ttctttgatg accgtcaaat cctctggatg gatcacaggc atgagatcaa gttcttttac
     1381 tatctcaatc aattcacttg gaaactcgcc ttttacagct ggatcaagga atgaacgatt
     1441 gaagaacgca tctgctattt gcgctgcttt aacatcttca gggtttgatt cgtctcttgg
     1501 atagcttggt gtcagattca agatgatacc aataacacca ccttgctcca tttcatggta
     1561 agatttgatg gctaacgctg aggccaacgc ttcatgatag ccaacttgga tcgctttttt
     1621 catatctgac tcttctggat aatgccatcg atataaataa cctgcttcga ctggaacgat
     1681 tggttcatta tgagtaaacc atttttttac tttcgaaccg aacaattcaa aacaactttt
     1741 ggcatatttt tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag
     1801 atacatcggc atatcgaaat gatacagatt cacaaagggt tcgataccgt tttcaatcaa
     1861 ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc
     1921 aggtatcaag cggctccatt ggatagatgt tcgaaatgaa ttatgcccag tctgtttcat
     1981 caactgtaca tcctcttgat acttcgtata aacttgtgaa gttttttcag gtcctacttg
     2041 gtcaaagaac ttttgcggtt cttttttgta ccaataatca aaaatgttct ctccttttcc
     2101 atcacctgaa atccttcctt ctgtttgtgg gccacttgct gcagaacccc accaaaaatt
     2161 tgctggaaat tgatattttt tcatcaaagc acctcttttt cttttttagt tcgttttagt
     2221 tgattccatg tatgatattt tccatcacaa ttactttttc attttcttcc tcttcttttt
     2281 tgattcgttt caacagctgt tctgcggctt gttccccgat tttatctgtt ggtggtgtaa
     2341 tcgtgatttt cggaaggatg ctcaaatcgt ttttatccaa ataatcataa tgactaaacg
     2401 caatatcctt cccgattctg ataccttctt gattgaaaca ttcgatagca ccgatcgaca
     2461 tattataatt gctgacaaaa atcgctgtta ctcctttttc taataattct tttgccccat
     2521 aatagccact ttttctagaa tagtctcctg taaagacctc taattggatc gatggatcat
     2581 tttgtaccgc ttcttttgct cctgctaaac gttctcttgc tacgtagtct gtttgagtgg
     2641 cagcaataaa tccgattttt ttatgtcctt gttcaccatg atgagcgatc acttttgcta
     2701 cactttctcg atcattcact aatattgtat caatacctgc tggtccttct gggcaattca
     2761 atgcgataac tggaatactc atttttgtga gcatttcaaa tccagaccac tcttcttcgg
     2821 ataagaagac aatcagccca tcgatcacat gcttcatcag gtattcaatt ttttgttcaa
     2881 ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta
     2941 ctctttcgat ccctgagaca acttccgacc caaaacggct ggaaatcccg tttatcaaaa
     3001 gtccaataga gaagcttcga ttattcttca accctttagc aatgatgttt tctttatagt
     3061 ccaacgctga aatcgcttct gctatttttt tcatgttctt ttccttcagt tgctgaccat
     3121 tcaagtaacg ggaaactgtt cccactgaaa caccagccat cttagccacg tcacggatcg
     3181 atgccataca ttctcactcc tgccagttac atttgatttc tctttcttgt tgttcgatta
     3241 tgatgaccgc tttctttgca tagtgttgct tgtcatcaat atccatataa ctaaagtctt
     3301 cagtagagca acttcctctc actattaatt atacttaaac aagtatttga accggttcat
     3361 ttttaatata gcattttttt aaaagattgc aacacttttt taaaacgctt tcctaaaatg
     3421 gcgaattttt tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg
     3481 tgcgtttttc tcttccatcc gtttgaagtt ttactttcca atggtataat ttctttatcc
     3541 tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac
     3601 ttttgacatt gactggctgt aatttgtttg gcggctctga ccaatcctcg gatgaaacaa
     3661 ccgattcttc tgtcttaaaa gcggaaaaag cagatatcac taacagtgta tatacgctaa
     3721 aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg
     3781 aatggacacg cacgtatcag acacaagaaa ctgattcttc tgaatcatct gaagaaaaaa
     3841 cagtgctgac agacatcaag attactacca aagatgacga atatattatt tcaggaaaag
     3901 aaaataaaaa agaagcaacc atcacattta ccaaaatcgg taattaccgt ttgaaggatg
     3961 aggatggcaa catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct
     4021 tcttttctgc tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat
     4081 agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa
     4141 ggaagcttga gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt
     4201 gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa
     4261 actcactatg gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca
     4321 tcagtaaaac aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat
     4381 ttcaaatatt tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt
     4441 aatttttctt tcccgtcgtt tggtttttta tttttagcct aatccttgac cttttgcatg
     4501 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta gtttttcttg
     4561 gatctgatgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
     4621 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
     4681 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
     4741 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
     4801 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
     4861 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
     4921 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
     4981 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
     5041 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
     5101 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
     5161 caaaatcact gacatacatc ccttcctctt ctataagtac tcgcctacgt aagtttcaac
     5221 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
     5281 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
     5341 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
     5401 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     5461 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     5521 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     5581 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     5641 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     5701 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     5761 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     5821 ggcttgtcag gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     5881 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     5941 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     6001 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     6061 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     6121 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     6181 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     6241 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata
     6301 ggatagacac cactatatat agtatttaga aaaaacattt ttcagctttt ttatagataa
     6361 aacaaaaata aataacgtgt tttcatcgat gtgaaatgac ttacttatag aagatactgg
     6421 gatttcgtcc tatatgccaa taacgtgtat tattttaaac aacaaatact tgtttctttt
     6481 attaacgaaa aaatcatttt gttctcgtgt tatgtttcac tgataaatga tgtttctcta
     6541 aataaaaaag ccgacgaact aaacgatcaa aatttgatca tttttgttca tcagcttctc
     6601 ctaaaaatct ttttgttaac gataaatagc tggatttgta aaaatatcac ttgaatcgtc
     6661 actgctggaa gaaaattcag tgataaccgc acctgttgcg cctgcctgga actagtgttt
     6721 gatattgggc tcgaacgtgt actgatctct agcatgaaga ataatttctt tttttactgt
     6781 ataataactt tctttccttt ttggaatagt acaggaaagt ttttttgttt cctttccttc
     6841 gacataaggt atacgagacc ttttcgacaa cgaaaggttc tcatttttcc ttcatcgtca
     6901 tttcaaggag ggtgccgatg ctctggacag tttcgatcgc tcctaaacca aaatctgtat
     6961 attcgattct ttctaactct gattctttca taccattcct gcctcaaggt acatctattg
     7021 tacttttttc ttgtaatcca ttctgcctct cctaactaat tgtcaaatca tcccaagata
     7081 tgtcttcttc ctctttttct tcttggatga tttctgcatt cggatctact ggttttttca
     7141 ataataaaat ggttattcct gtg
//
LOCUS       DACEPW010000126         7097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEPW010000126 DACEPW010000000
VERSION     DACEPW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.128"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(57..350)
                     /locus_tag="IW819_002041"
     CDS             complement(57..350)
                     /locus_tag="IW819_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1245136.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="IW819_002042"
     CDS             complement(378..1043)
                     /locus_tag="IW819_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1245137.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="IW819_002043"
     CDS             complement(1183..1815)
                     /locus_tag="IW819_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1245138.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="IW819_002044"
     CDS             complement(1822..2889)
                     /locus_tag="IW819_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1245139.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="IW819_002045"
     CDS             complement(2886..3617)
                     /locus_tag="IW819_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1245140.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IW819_002046"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IW819_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1245141.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IW819_002047"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IW819_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1245142.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5780..6949
                     /locus_tag="IW819_002048"
     CDS             5780..6949
                     /locus_tag="IW819_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1245143.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         2139 a         1452 c         1095 g         2411 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt actataagat
     5701 taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt aaaaaagaaa
     5761 ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa aatattcatt
     5821 tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa ctattcttgg
     5881 atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat cagctcaaaa
     5941 aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc aactattaag
     6001 aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca tcatggaaaa
     6061 aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc tttattatca
     6121 gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag tcgagcgtgg
     6181 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
     6241 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
     6301 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
     6361 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
     6421 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
     6481 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
     6541 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
     6601 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
     6661 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
     6721 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
     6781 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
     6841 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
     6901 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
     6961 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
     7021 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
     7081 acagaacaga aagaggt
//
LOCUS       DACEPW010000127         7000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEPW010000127 DACEPW010000000
VERSION     DACEPW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.129"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..220)
                     /locus_tag="IW819_002049"
     CDS             complement(<1..220)
                     /locus_tag="IW819_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HAQ1245144.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLC"
     gene            548..1957
                     /locus_tag="IW819_002050"
     CDS             548..1957
                     /locus_tag="IW819_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1245145.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            1954..2682
                     /locus_tag="IW819_002051"
     CDS             1954..2682
                     /locus_tag="IW819_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1245146.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(2810..3721)
                     /locus_tag="IW819_002052"
     CDS             complement(2810..3721)
                     /locus_tag="IW819_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1245147.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(3738..4745)
                     /locus_tag="IW819_002053"
     CDS             complement(3738..4745)
                     /locus_tag="IW819_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1245148.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(4742..6871)
                     /locus_tag="IW819_002054"
     CDS             complement(4742..6871)
                     /locus_tag="IW819_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1245149.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(6876..6983)
                     /locus_tag="IW819_002055"
     CDS             complement(6876..6983)
                     /locus_tag="IW819_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245150.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2159 a         1449 c         1198 g         2194 t
ORIGIN      
        1 tgcagaggcg ctgatctaaa aaggcgcgat caatctcgcg atagcttggc aattcttcat
       61 aaaccgcatg aagtaacact ttaagcgtcg tttggaagtc tagtttttta ctgtagaaat
      121 caaagtcgct aattaagctt tgagagtttt ctgataaacc agaaaaattt attgcagaaa
      181 accatttttg aaatgaggtt tttacgttat acttatccat gcgtgagtcc tttttattgg
      241 tcttggataa gtcatccgag aatagtataa aggacttttt tgcgtttgga tagctttgta
      301 ttaggctttt gatgggttaa aaagtctttg caacactagt aatttagtat ttatgtataa
      361 attgaaagat attttaccac cacttagtct tcttaaaagt gatccaacat tttgtttgtg
      421 gcagaattta actacacact aatgcagccg aaagtacaca actatgcagc agtttgactg
      481 cgaactggtt tccttccatc ataagtttga atgacttagt gatggaggta cagaaaggtt
      541 gttaactttg gatgatatta aaacaataaa acatttgaga aataatcgtg atcagtcaat
      601 tgatcagata agaaaagcaa tgaatatcaa ttggcgaaca gcgaaaaaat acgccgatag
      661 tgatcaacta ttcattacag atcccactcg aaaaaacaaa tccatgatgg atgatggttg
      721 gggagaaatc atcggtagtt ggctagaaga agacctacgt ttacgcagaa aacttcgtag
      781 aacggccaaa gctatccatg aacaattgaa acctttgggt ttccctggct cctatcgaac
      841 agtttgtgta tacatttctc aatggaaaaa gcagcgttac gacgaaacga attaccaagc
      901 tgtcgatcgt cttgatcatc cgatcggaga agcacaggtc gattttggaa caatggaagt
      961 tatgcatgaa gatcacatga aagatataca acttcttatc atgtcatttc cctatagtaa
     1021 tgcgacgtcc gcaagtgcca tgccagctca aaaccaagag tgtttcctgg aagcattaaa
     1081 acaactattt aatcaatgcg gcggcgttcc aaaaacgatc agaatagata acttaactcc
     1141 agcggtgaaa aaaacaaaat caaaatttga agaagcacaa ctaaccgatg aattcttacg
     1201 tttcaccatt cattatggtt gtgatgttca agtctgtaac ccacgtagtg ggcatgaaaa
     1261 aggacatgtc gaaaataaag tgggttatgt ccgatacaat ttcttctcga cttcgccaat
     1321 catggactct tttgagtccc taaataagca acttgaagaa caattaaaag cagatcgaaa
     1381 aaggacgcat tattcaaaac atcaattaat cgaagatttg tggaagaaag aaaataaagc
     1441 gttacattct cttccacaag aggattaccc aatttttaaa gaagtggaag ctaaactaaa
     1501 tcgttatcac gaaatgaaat tggatcaaac ggttattcat attcctaaag catatggcta
     1561 ttctcaattg actgttgttt tgacttggaa taaattcaaa gttcttacgc ctcatggaga
     1621 aatattaatg aacgaatatc gcccctatat gaacaaaaaa agattgatcc catggagtga
     1681 catcatgaaa caatggagta ataaattgaa tgtcgttagt tattcgaggt attggaagta
     1741 cttgcctgca cgccttcaaa attatctagc gattgataat aatcacctga aatacagacg
     1801 gataaaacaa ttactaagtt tattagcgac ctacgatatc caacaaatta atgaacaatt
     1861 ttacgaatta gtcgttcaag aagacgcccc ctctgacttt gatgtgaatt ggtcaaacta
     1921 tgatgcatta acaaaaacag gaggtgtgca aaaatgattg gacagacgtt agaagattat
     1981 tgtaaaaagc ttcgattaag ccatatcatc acgaacatac caagagcttc tttcgaagct
     2041 ccagaacaat ttattaccga tttattccgt ttagaactgg agaccagaca gcagatcaaa
     2101 ttagaacgga tggaaaaatt agccaaattt cctgtaccaa aatcgctaaa gaactatgaa
     2161 tggcacgaag atatttttct accatctcat ctagataagg agacccttgt ctctcttgat
     2221 tttctaaaaa ggcaagaaaa cattgtttgt gttggctctc ctggtacagg gaaaacacat
     2281 ctagcgatcg cattagggag aaaagcctgt agtgaaggtg tttcaaccaa atttttccga
     2341 gttaacaatc tcgtagagca attagagaat gcattaaaaa aacaacagct agccagtttc
     2401 aagaagcgat tcgaaacaac agaattaatt attcttgatg aaatggggta cttaccattg
     2461 acaaaagaag gagctgaact tctcttccaa ctgatttcag attggtatga gcaaaagagc
     2521 attattatta cctctaattt agaattcagt caatggaatc gagtgtttat ggattctcga
     2581 ttaacagctg ctctcgttga tcggctaatc catcacgctc acattatcag cttcaaaggc
     2641 gaaagtttca gattgaaaaa ttcattatct aaatcatatt gaaggaaaaa actagttgca
     2701 gcaaaactct gcactttcgg ctgcattttt ttgtaaaaag ctattgcaca acacaaacat
     2761 ttgaacgacg taattccaaa tgttggattg cttttagata atcttttatt tattcacaat
     2821 tgctaatagt ccaattttca tttttgtata gttttaatct atactgaaaa acacttgttg
     2881 ttttggacaa ctcatcaaga tactcaacac caacaatcac ttgaatcgct ttaccctttt
     2941 gtttgtaaat aggattcact aattccacaa atttcaaatt agtattaatt ggttgcatcg
     3001 cgtctttttc aacataatgc tctagttctt tttctgaggc tgctggataa agcctgaaaa
     3061 acgtctctaa aaactcggtg atttcttccg ttgtctttga atcaattttt gaatcacttt
     3121 ttacttgtct tactttatgt tttgcctttt ctggcttacc aatcatagtg ggtaaactcg
     3181 ttacaatatg attgcctttt tcatcttcaa agatcatcag acgatatgca gaagaaattg
     3241 attgtgtctt tttcccttct gtaatctttt gatctaccac aaatgaaacg acaaattctt
     3301 ccgagtgttt ttcaacatct agaattttta ctgatacaac ctcggaacta gtgggaatat
     3361 ccgctcgaac catatcttgg ctcaaagcga gcccttcttc tgtaaggtac tgctcaagat
     3421 tagtcattct cttttcaatt acctccttgt tattcttcca tgaaaaatat tctttcacaa
     3481 aatctacagt aaaattttct actccagagg tattcactat tttctctttc acgaccactt
     3541 tctcatgagt tgtatgctga tcaatggccg tgaagttttt gtagattcca aaagcaaggc
     3601 tacaagacaa taaaagccaa agggcaagca ccatttttcg atgcttgccc acggatacta
     3661 ctcgttcttt cttctttttt ggaatctttt ccttgttact tcgctcaatt tttatcttca
     3721 ttttccattc ctcttttcta ttgtttatac cgaccagcac caataatgtg ctgttgccaa
     3781 tagctatcgg taagattggt ccaacccacc ggatctcctg catgaaacat tctgttctct
     3841 cctgcataaa tgcccacatg agtcacataa gtaccggcat cataagttga atgaaagaaa
     3901 atcaaatcgc ctggtttggc atcttttaag tcaatatgag tcatagcatc atattgatcc
     3961 tgagccaccc gagggagttt caaaccaact ttggcataac accattgagt taaaccacta
     4021 caatcaaacc cagttccggg acttgaccca ccaaatacat aagccgttcc ttcatacttg
     4081 aacgcctcgt caaaaattcc ttggactgtt ttatcatcaa actgtgttgt agttaggtac
     4141 tgcttcacta ataagcaata aaacatattg ccatagttat accgccaccc actattttcc
     4201 ttaatcgcga tgggattggg ataatcgact ttttgaccac ccgatttttc ttttgaaaaa
     4261 gattcggcta aagcaaaact atgcttcttt ccgtttttcg cgacataatc caaatacccg
     4321 tcaccatagt tataagcctg aatgatacta tcttgatcgc atcctagttc tttggaacac
     4381 ttaaccaaag acgaaaaata gagacacact tgtttgatgg atgcctcggt acttaatgaa
     4441 ttcggtgata aacctgctga ttcagaactt tgcatgacat cttccattgt acctccagat
     4501 tcgacttgaa taatcgccaa aagaatcgaa acatactctg ttatatctga ttctttggca
     4561 tacttttcca ccattgaacg gtgtttcaaa actacttctg agacactact acctccagga
     4621 gaagttccag aactaccact tccctcaccg tcctcaccaa acaacaaacc cacacacatc
     4681 aagatgccca caaaagagat ggtcaggaag gttaacgaaa taatactcaa tttttttaag
     4741 ttcatctttt ctcacctggc tttggttttg ggcgagtgtt cttttttctt tgtacttgtt
     4801 gattggttcg ctttggaaaa gtgaatctct gtttcacttg ttgaaaggaa ttgttcttgc
     4861 ttagtggctg agtgcttccg tttggaatga cttcttgact agaaagtttc cgtttgattt
     4921 caggattcga atgtacttgt acccttggtt ttaaagacct attttctggt agttcatttt
     4981 tctgtggttt tatctccttt tttatcggaa cattttccct tcctttctca tgacgattcc
     5041 atgcttcgtt caatactttg cgtttttcat ccatttttgc gtctcttcgt tgtcgttgtt
     5101 tttctcgcaa ctcccctcga tttgcttttt cttcgaaagg ccctcgctta aagtcctctg
     5161 gtactttttt cgtcttttcc atgcctttat gaaggctgta tttcaagtta gttggtgcat
     5221 cggttacttg attttttgct tgtttcgctt tgtccttcac gatttctttt gtatcgagta
     5281 cttttccaac ttttctacca actaaattta ttcgtgattc tttcttcgtg cccactggtt
     5341 gttcttgttt tttcagatca gaatttagct cgttaatgct tctcgcggaa tggttctttc
     5401 tattggacac tctattgatt tttgaactac ctttcccttt tgtagaactg gtagctagtt
     5461 tcccaccaac tgctgcaccg ccaccaaaag caaaagttcg acccacattt cgattaagct
     5521 gtcgcaattt tcgattcatc aacatttgcg gtttccgcat gactcgcctt cccattgact
     5581 gactatcatt actttgaagg ttaaacatgc tcataatgtc acctaatttg aaatagatac
     5641 cggcaaaggt cacaatttgt agaaaagcta ccataaagaa gggataggtg gccgaaatat
     5701 tgaaaaacat cgtggagata ctgaatgcag tcgtaatgac taaggtaact cccgctcgca
     5761 tcataatcgt attgaacaaa cgaataatcg cccttttgcc caaactctca tacgtcggca
     5821 acatcgacaa taagaaacta atgggcagaa acatcgcaaa gataatgaaa aggatttgtg
     5881 agaacaacat aatccctgtc agaagaaaca caaaaaagga aatcccaata ttaaacaaca
     5941 ctaaaaagac cacggttcct aaacggttca tggtcttggt aatactgaga ttcgcattgt
     6001 cattatcttc aatctctgct ttgactgctt cttcccgatc cttaccctta ttttcatccg
     6061 gactcacgga taaaatctta ttaacccgac cctcgccaat ttcttctata ttggaatcat
     6121 cgaattgtaa tagtaaccag ggttgttgaa cttgaatcga aaataaactg tctcgaatca
     6181 aatccacact atcttttccc tggctttctg aattaggtac aacaatcttg gttccaaggc
     6241 taagagccgc ttcactaacg tctgagctaa agtcattgat ttttgtaata taggtgggcg
     6301 catacgcaat gaaagaaccc gagagtagaa aaatcatcac aaaattaatg acggttcgga
     6361 ctgctttggt agtttctcgt tttaataatc cggtatacgc cacataaact cctagagcta
     6421 aaatcatcaa taataggaac ccaaagtaga atcccgaagt ctgtaatcca ttttcagtga
     6481 taccggctaa agtttggata ttctttccaa tacttttagc cgtatccgaa atgaaatcca
     6541 acttgtaagc ttcttgaacc acatagcccg tggcgtttga taaatacaga cttactgtcc
     6601 aaataaaatt ggtaatggca taaagaccat acatcacgct tttccctaag ccatcccccc
     6661 aattccaagg taaccaatcc caagaactat ccacaaagaa atccagttga taattgttca
     6721 gagaatattt ggaataaagg tttccggctt caactgtatc atcaaccaga cctgtcgcct
     6781 cagctaccgt accgactaaa ctcaatagaa ctgtcaatag gacgatgcta ataaaaacga
     6841 ttcccacaat ccgaaaaagt ttctttttca tcccctcact ccattctctc ttgaaccggc
     6901 ggacgagtgt caaaagcatg gaagagttct tcaaagactg gatgaacttg aaccacacct
     6961 actcgcccat acaagtcttg catcaagcat tgaccgtttt
//
LOCUS       DACEPW010000128         6905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEPW010000128 DACEPW010000000
VERSION     DACEPW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.130"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..425)
                     /gene="tsaB"
                     /locus_tag="IW819_002056"
     CDS             complement(<1..425)
                     /gene="tsaB"
                     /locus_tag="IW819_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1245151.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVD"
     gene            complement(514..1494)
                     /locus_tag="IW819_002057"
     CDS             complement(514..1494)
                     /locus_tag="IW819_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1245152.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(1518..3002)
                     /gene="galT"
                     /locus_tag="IW819_002058"
     CDS             complement(1518..3002)
                     /gene="galT"
                     /locus_tag="IW819_002058"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1245153.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(3014..4003)
                     /gene="galE"
                     /locus_tag="IW819_002059"
     CDS             complement(3014..4003)
                     /gene="galE"
                     /locus_tag="IW819_002059"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1245154.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            4251..4418
                     /locus_tag="IW819_002060"
     CDS             4251..4418
                     /locus_tag="IW819_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1245155.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(4480..6291)
                     /locus_tag="IW819_002061"
     CDS             complement(4480..6291)
                     /locus_tag="IW819_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1245156.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(6288..6653)
                     /locus_tag="IW819_002062"
     CDS             complement(6288..6653)
                     /locus_tag="IW819_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1245157.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(6816..>6905)
                     /locus_tag="IW819_002063"
     CDS             complement(6816..>6905)
                     /locus_tag="IW819_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1245158.1"
                     /translation="KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
BASE COUNT         1893 a         1460 c         1223 g         2329 t
ORIGIN      
        1 ttatccacac cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct
       61 ttatctaatg tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa
      121 gaaccgggac catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct
      181 gcaaacaatt ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt
      241 tgtatttgtc caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca
      301 gtatctattc ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc
      361 attgtagcat acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaataa
      421 tacatgatta tctgctggcg ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg
      481 tatcactcca ttttcccaca gcttttccat cttttatttc gttgattctc gaataatcaa
      541 ctctgtttcc acaataatct ttcttgaaac cggtgctttg gaattactta attgaagaat
      601 cgtctccact gctagctctc ccatccattc ggtataaact tttacagtag aaagcgaggg
      661 cgttacatat ttcgctacac tgacatcatt gaagcccatc acagaaatat cttctggtac
      721 cactaaacct ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat cattagctgc
      781 aaaaagcgca tcaggaacat gcggattcgt ttttaaatac tgcttcactg cttgatatcc
      841 tgactcgaca gaaaaatctg cttcgataat tggaccttcg tcgtagcctt tttctgccaa
      901 aacttgttga aatgccataa ttctgggatc ttttagccgc tgatggtttt tcttcgtata
      961 ttctgttcca gcaagcatag cgatatgttg cgctttatgg gagacaaagt gttcaatgac
     1021 tttttgaaca cttgacttaa agtcgacaac tagagagtcg attccttgag aagtgccgtc
     1081 agaatctacc aaaagcaagg cattttggga tcctttcatt tctaataact gagcttcgtc
     1141 gaattttcct agtgcgatcg ttccatctgc cggaagatca ctaatggaat cccacgtttc
     1201 tttcaataaa gtaacgtttg cttcttctgc ctttttttcg attcctaggc gaatagataa
     1261 ataataaaga tcttcaagct cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg
     1321 cttagtattt gctgcatttt tacgatgttt cgtataattt aagcgttctg ctgcttcaaa
     1381 aattttttgc tttgtggcta agccaacgga aagatcagga tcatagttga gaactcttga
     1441 aacagtagca ggagaaactc ctgccaactg cgcaatgtct ttaattgttg ccatttgtct
     1501 ctcctccttt tttacgttta taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt
     1561 tcatctcgct tgaatactcc agcgtcttcc agtacacgtg caaaaacctg acctacagat
     1621 ttttgtacga catcggtgac cgtatctttc gtcaaatcag gatgttcttt ttttaattgt
     1681 tctgcccacg gacgatgata ttcagccatc tggttttctt ctcctaataa gaaatgctct
     1741 acttctacta gttctggttt caaacgaggt ggtaaaacag caagccccat tacttcgatc
     1801 aacccaatgt tctccttttt gatatgttgg acatctttat gtggatgaaa aatcccatca
     1861 ggatgttctt ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc
     1921 cttcttcgtg caattggcgt aatcgtatgg tgtggtgtac catctgctga aaaagctctg
     1981 atagatactg tttcatccga atatgctttc catttttcca atactgagtt agctgcttca
     2041 ataagcaagt ttttctctgt tgattgcaaa cggatcacag acatcggcca tttcacgact
     2101 cctgcgatga cttgcggata atcttctagt ttgataagtt tttctatcgg tgcttttgcc
     2161 ataggaaatt catgacgtcc tgcctgataa tgatcatgag aaagaatcga tcctccaaca
     2221 attggtaagt cagcattcga accaacaaag taatgaggca agacttcagt aatccgaagc
     2281 agtctagcaa atgtttcctt tgtgattttc atcgggcggt gttgttctga taaaatgatc
     2341 gaatgttcat tgtaataagc ataaggggaa tattggaatc cccagctttc accgtctaga
     2401 ttcattcgaa taatccggtg attggtgcgg gctggatagt tcaagcgacc tctatatcct
     2461 tcgttttcca tgcaaagcat gcaggtagga tagtctactt ttaccgcctc gcgttctgct
     2521 gcgatttgtt ttggatcttt ttctggctta gataaattga ttgtgatttc cagttcacca
     2581 tactccgact ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca
     2641 tttttcttac ttaaattaaa gaaatactca gttgcttcct ctgggctttt cgaatagtgc
     2701 tgtgcaaaga atgcattgac gactgaaggt ggcggtgtga gaaaatccat taattgcgct
     2761 tcaaaaatct cttgttctgc tggcaattct gaaagaacac cattttctct tgccttagcg
     2821 acaagctgat ccaataagtc tacagattct tccttaactg gtcttacttc aatgctttct
     2881 agagcatctt ctcctaccat ccctaaaaga cgattctgta aatataagcg gtccatttcc
     2941 atccatccgc cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac
     3001 atatctcatt cccctattct tcataaccat ttggatggct gacatgccaa tcccatgctg
     3061 ttttgataat cgctttgata tctgtgtatt caggtttcca gcccagaatc tctctcgctt
     3121 tttcacttga agcgactaat cggctaggat ctccagctct tcttggtgct atttctgctg
     3181 gaatttcttt tcctgtcact tctctagctg cttctagcat ttccttgact gagtatcctt
     3241 tgctgctgcc taaattgaag aagttgcttt catttccttc tttcaagtat tccaaagcta
     3301 aaatatgtgc agcaatcaaa tcttctactt gaacataatc tctgatacat gttccatctg
     3361 gtgtatcata atcgtctcca taaacagcca aggcttttct ttgaccaaga gctacttgta
     3421 aaatgattgg gacaagatgc gtttctggtg tatgatcttc tccgatagat gcatctgctt
     3481 ttgctccggc tacattgaag tagcgcaaag cgacatagcg catgccatat gcttggtcac
     3541 accacttcat cattttttcc atcatcaatt tactttcacc atacggattt ttaggattag
     3601 ctggtgtatc ttctgtgatt ggagactctt tcggctctcc ataagttgct gcagtagaag
     3661 agaatacgat tttattcacg ttgaactcgt gcatgacttc aagtaagatt tgcataccgt
     3721 atacgttgtt gttgaaatac atcaatggtt tttcgacaga ttctcctact aaagagcttg
     3781 cagcaaaatg gattactcct tcaatcggtt ccttttcaaa tacggaacgt agaaattctt
     3841 tatcccggat gtctccttca taaaaatgag catctggatg tactgcttgt ttgtgacctg
     3901 tgagcaaatt atctaccaca gctacttgat accccttttg aactagctgg tctacagcgt
     3961 gcgaaccgat ataaccggct ccgcctagta ctaaaattgt cataaaaaac actcctttga
     4021 ttagtaaaat tgtgtttgtt gattctatat aatttaaaaa acgaagttta cttttatggt
     4081 gaaaacaaac aaagtaaaat ttcgtaaatt catatggtaa atttataata aaacatttag
     4141 taaacatttt caaggatttt ttgcaaaggt ttacaaacaa acgtaaaact agcctttttt
     4201 tctcttttgt tccataatag atataacaaa taataaatga ggtggattca atgaaaaaat
     4261 ttgtatctgg aatattagta ggaagtctgg caacagccgc tgccgttgct ggtttggttg
     4321 cttctgtgaa aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg atcgaagaaa
     4381 accgtaaaaa agcaatgcgt aaacgcgttt ctcgttaata attatttaaa aatacgcatg
     4441 aagactttct aaaaaacaga taagtcatca tgcgtatttt tatttgtcag tcaatataag
     4501 ttgtgctatt ttatctgcct gtctcagatt ccatggatct gtataaggtc tagctactgc
     4561 tgcagttagc caatccacac cttcaacagg tattccatag caataaaagt cagactggat
     4621 ttcccctgaa gcatcaatca cgcgattcgt acctcgctca acatctatag cttctgaatg
     4681 ataacttcct gtattatctg aaaatgaaaa agggcgaaca agtttgtttt ccttcaaatt
     4741 ttttatgaca ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag cttcaatcaa
     4801 atagcgactg ctgatttctt tatcttgtag agaaataaca tagcggttgt gcttttgctg
     4861 aatatcaaat ggcggttcga tcaaatgaaa aatacccgca tctatcagtg ctgctaattc
     4921 tttcgtccga atgattggcg gaccgatcgt taaaaagcta tttagatgag taaaccatcc
     4981 ccatagtatt tctttgtatt ccttagcaga aaaaacgtgc cattcaatag cctgccggat
     5041 tggatcacgc cagtctttta atgcatcaaa tgttgaagca aatgctcctg tgcaattgcc
     5101 tttttccgct tcttttatct gcccttcaat aaatattcgg ctagcttctg aaaagggctc
     5161 attctgatag gcaagaggag tttctaaata cgaccaatcc catctataag ggattagtgc
     5221 tggatgcttt ccgagacact cctctgaaga gagggagaga aaatcatgct caaaattttt
     5281 ctttggaatc ggcaagtgat attcttcgat gattttcttg taataaaaca actcaacgtc
     5341 ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat gccattgctc
     5401 caggttttca tttgttagta accgtggcca tgccattgct ccgccttgct tttgatttct
     5461 tcctcgaggg taataaggga gcccccttct tgatccactg tatacaatcg gttccttgcc
     5521 tgatggatga tagaccagtt ttcccttttc gttctcgaag cgccctcctc ttgatactgt
     5581 aaacaatccg acataatcaa aaaaacttaa gcctagacct cttaaaaaga cattttcttt
     5641 aggcggtatc ttcgaaagat ctacatctgc aggattagaa ggaagctgat acagtaaaac
     5701 atttttcttt gcgtagtcta cccacactct ttcttcttct gtctcatcat tttcccagtg
     5761 accgcttgct aaaacaagct gatcgataga atagctttgc tgatctgtct gtaaaagata
     5821 accatctttt cttttgctta ctttttttac taaagaataa acaaactgaa tagaagctgg
     5881 aaattcttct tgcaactttg agaaaaacca gcgttgatag agtccgtata aacatcgagt
     5941 agattgctga tctttttcca gacgattgat ttcttctata attagttgct tatttttggt
     6001 taccgtctgt tcgatataat ctgtagcagg tcctttactc cattgaaaca agttgggtcc
     6061 ctgtgttacc aatcctccac tggacaatgt ttcatctgta aaaagcgtca cttgttgaga
     6121 aacagaattc attaataact ccggtggctg attcaaccgc caaactcgac ctccaggacc
     6181 attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc tgattattat ggagaatcct
     6241 ttctgctaca cttaatcctc taggtccggc accaatgatc cctattttca tttgcatgcc
     6301 tccatttcga taaatttttt caataaaaca gcatggttct gatgttcatt ttttgcacca
     6361 taaacaaaag taatgactgg atgttctttc ataagttgac tgagtttaaa aatagcttgc
     6421 ttcttttctt cttcgaaaat caattcttgc tggtatttct ttgcaaatga agcaaatcgt
     6481 tctggtacat ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc tttcaaccac
     6541 agatctaact gcgctttttc ttttgaaatt cctcgtggcc agaatctatc cacgagtatt
     6601 ctatagccat cctctttttc agcatcaagg taaacacgtt ttaatttgat catagttgtt
     6661 tccttctttc taccttttcg cttagcttat atatgtagat aatagcacaa aataaaaaaa
     6721 atgaacggca caactatcca ttgcgccgtt catgtgatct tggtcaaaat ctaatgattg
     6781 attcggtttt taaagctgtc atacaatcat ctgttttata cttttttact tttgattttt
     6841 ccagaccatc cgtcatatcc acccttaagt aaataaatct cttgataacc tgctttacgt
     6901 aattt
//
LOCUS       DACEPW010000129         6835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEPW010000129 DACEPW010000000
VERSION     DACEPW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.131"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1477)
                     /locus_tag="IW819_002064"
     CDS             complement(<1..1477)
                     /locus_tag="IW819_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1245159.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTEL"
     gene            complement(1561..2364)
                     /locus_tag="IW819_002065"
     CDS             complement(1561..2364)
                     /locus_tag="IW819_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1245160.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(2367..3149)
                     /gene="phnE"
                     /locus_tag="IW819_002066"
     CDS             complement(2367..3149)
                     /gene="phnE"
                     /locus_tag="IW819_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAQ1245161.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(3146..3925)
                     /gene="phnE"
                     /locus_tag="IW819_002067"
     CDS             complement(3146..3925)
                     /gene="phnE"
                     /locus_tag="IW819_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAQ1245162.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(3897..4688)
                     /gene="phnC"
                     /locus_tag="IW819_002068"
     CDS             complement(3897..4688)
                     /gene="phnC"
                     /locus_tag="IW819_002068"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245163.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(4749..5678)
                     /locus_tag="IW819_002069"
     CDS             complement(4749..5678)
                     /locus_tag="IW819_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1245164.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            5873..6556
                     /locus_tag="IW819_002070"
     CDS             5873..6556
                     /locus_tag="IW819_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HAQ1245165.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
BASE COUNT         2018 a         1487 c         1297 g         2033 t
ORIGIN      
        1 ttaattcggt catgtcgatt tggatttctc ggacaatttt ctccgcacca aacatcgcat
       61 aatttcctcc gccgattgcc cgatattgat ttgtcaccac ttccagcatt tgttgcggaa
      121 gaacggggtg acctttaaaa cgaagttcgg taatccgttg tccaacaggc tgtttcatat
      181 caatagtgta ctgaatccct tcatacatgt cataattgta gtactggggc ttaggctgaa
      241 taaaggctgg attgaatacg atttggttat tttctacagc taaataattt gcggtctgct
      301 ccaacgccgc tctcaaatct gcaccattta tttgaatcac tgccagagta ttcggataaa
      361 tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc aaagcctttt ccttcattat
      421 tgaataacgc agttccagaa atctctgagc cgctggcaaa taattgtaca cgattaataa
      481 actctacata gggatgttcc tcaagacgag cctgcatcgg atcattaata cgcatatttc
      541 cgttcacttt acctaagggt tgatccaacc atttctctag tttttcttct accggtacga
      601 tcgctgcaag aatttctgga tcaggctctg atttttcaac tgagtgtaaa gcaacctggt
      661 gatctgtaac aaccacttgc ccctcttttt tctcgacggt aagacgaatt tcaccgatga
      721 actcgccacg atagcctggt tgaacaacgg gaactccatg aaccgtttca gcaattttcc
      781 ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac ctgctgcaat agctcgtagc
      841 cttcattctc tccagttagc gcttctgtgt cctcaccggt taacaagtct ttttcaaatc
      901 cgccgtggta actaacgatt accaagtctg cttgtttcct taatagagga acatattctt
      961 ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa ggtttctggc ttttcccagt
     1021 gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt tacacccacc ttttcgataa
     1081 tcttataggc gtttccaaag tatggttggt catttttgtt caccatattg gctcctaata
     1141 taggcgcttg ataattctta atcgcttcct cgagataatc cacaccatag ttaaactcat
     1201 gatttcccaa cacttgaaca tcatagctca tttggttaat caagtctgta agctctttgg
     1261 ctgaaccata ttctttagca agataataac tcaaaggaga cccttggatg aaatcaccat
     1321 tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa ttccttcagt ttcgacgata
     1381 ctttagccgt tcctaaggcc agatcggttc tgccggaata gtctgttggt gaaacatagc
     1441 catgcatatc acttgtctct aaaaacgtaa tgttcattct gcgtgctcct ttagctgact
     1501 caaaatagaa aaaccgaagc gtattcagct gttcggtctc tttttttgat ttataatgcc
     1561 ttaatccttc atttgaagtg tttttaaaat gttgcctgga ataaagtttt ctgttgtctg
     1621 gcgaacaatc cttccttgat cgagatgata aaggttatac ccatgacgtt cttcaaagcg
     1681 tacgaagcca tcgccttcat cttcaccaac aaaggacata ctcgatacag taaaataggg
     1741 gatcccatta atttcatcag taaatgtgtg atgggtatga ccgtttagga tacagcgaat
     1801 atcgtaaggt gcgattagct tcaagaaacg ttcggtacca ggccattttg gtatactgct
     1861 ttggtgagac agtagatgat gatgggtgac caagaaaatc ggttggtctt tttcttgctc
     1921 taaggctttt ttcagccatt gaaattgctg atcgtccact ataccatctg cgtatccata
     1981 gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat ttgactatct ggttgtacgg
     2041 ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa tgttctttga tatcgtgatt
     2101 gcctagagta acaagaatcg tcgtttctcc tagcgcattt tccagccaag tcttcaagta
     2161 tcgataatcg tctatttcgc catcttcggt taaatctccg ctgataatca caaggtccaa
     2221 gggtgtttgc tgcaggagat gatgaatgca ttgctccaga gggataaggg gattctgcat
     2281 cgtagccagc attcccttat agccttcttc acaagcctca tactctctgc gaaagtgtat
     2341 atctgatata tgcaatatat tcattcttat ctcaatctct ttcttataat tccggttatt
     2401 tgatctaaaa taaacacggt tacgactacg ccaatcaaaa taatactcac tttgctccaa
     2461 gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc cgccagcgcc aactaagcct
     2521 aaggtagccg cactacgaac cgctatttca aaatgattta atgctaaaga acaataaatc
     2581 ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc aaaaatttgc tcctaccgca
     2641 tgcatttctt caacaagtcg ctcatccatc gcttccattt cttctgtaaa aagtttgcca
     2701 agcattccga tctgatggac gccaatcgcc ataacaccag caaaggggcc aggtccgacg
     2761 actttcacaa aaataattgc aaaaactagt tcaggaaaag ctcgtaaaac attacagata
     2821 agcttgccaa tttttgttac ccatggatgt gcttgccaga ggttaaccgc actgatcaag
     2881 gtaatgggaa tcgctaatac cgtagcgatc attgtcccta aacaggcgat tccaatggtt
     2941 aataatagca aactaatcaa atcctcgccg ctgccatcat atagaaactg ccaatcaggc
     3001 tgacctaacc ctctcaaaac gtccattccc atggaaggag agaaggtgct taatccagag
     3061 taatccagag tagcggccga caaaataacg agaatcacta aagcaactaa tgataaaagg
     3121 gttcttctgt tcatttcacg aagcatcata acagggacct cctgacacgt tcacttaatt
     3181 tatcaacgat cgcgacagtt actaggatcg ttagaataat gattgaaact cgttcataat
     3241 tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc accagctccg acatatccaa
     3301 ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa agcatagctc gcaaactgac
     3361 tcaaaacttg tggagcgatc gaccaaatag cgatttgcat tttatttgct ccaacagccg
     3421 cagcggcttc aatcgggcca aagtcaatgg tttcaattgc ttgaaacatt aattgtgaca
     3481 ccataccaaa agtaaagaca gcgatagtca acacgccagt cgcttcacca attccaaaca
     3541 cagcaacaaa aagcgcggct aaaagcaggg tagggatggt ccgtactaaa cctaaaagaa
     3601 aacgaaagat ggtagaaaga agacggtttt ctgtcactac agtggtcgct aaaaaactaa
     3661 cagggatcgc taaaattaag ccaatgaccg ttccaagcaa agacatttga agtgttttca
     3721 ccataggctc gactaattta ggtaaatagc taaagtctgg atgaatgaag cgtgataaaa
     3781 agacagtcat ttgattggag ttttgaaaaa cctgagaaaa atcagctgac gtgatccagg
     3841 cacttccttg aaaacaaaca attaagaaca gaagaataag caggtgttta taccatttaa
     3901 agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct tttcttcaaa gattgcattg
     3961 ccataaattt gctctaagcg ttcatcggtt aagtcgtctg gacgaccatc aaagacaacc
     4021 tgagcagctt ttaacgcgag cacacgcgta gaaaactcac gagccagagg gactgaatgc
     4081 agattgacaa tgactgtttt atccatcgtt tgattgatat tttttaggtc catcataacc
     4141 ttttgagtcg tgattggatc taatgatgca acgggttcat ccgctaaaat aatgggggct
     4201 tgctgcacga gtgctcgcgc aatggatact cgctgttgtt gcccaccact gagctcatca
     4261 ctgcgtgtct gcaatttatc agacaaacca actttttcca gtgcttcctt tgtctgctgg
     4321 tagtcttcct tcgtaaaaag acctaaaata cttttccaag tggagtaata gcccaaacgt
     4381 ccagatagca catttttctg aaccgtacta cgctttacca gattgaagct ttgcgagatt
     4441 agaccaatct ggcggcgaat ctgtcgcagc tcttttttat tcgcttttgt aatagaggtt
     4501 ccattaatgc gaatatctcc tttggtggtt tctgccaagc gattgatact gcgcagcaga
     4561 gtgctctttc cagcaccact caaccctatg atggaaacaa attctccatc ttcaattgtt
     4621 aagtcaatat ccttcaagcc aacaacgcca ttgggataga ccttcgaaac attgtcaaaa
     4681 ataatcatta tccttctcca tttttttaag tgtaagcaaa aaaaagcttg ctgacactta
     4741 ctgatgtttt attctgctgc ttttttcgtg tagtctctga tcgtgtcata ggcagaatca
     4801 gcagcttctt catagccctg atgcccccac aaagccattg cttctttgcc atctttatca
     4861 tccttcatat ttaagaaggt ctctttaatt ttcgctttta aatcttttga catgtcaggc
     4921 tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca ggtacaggac ttttaaatcc
     4981 ttaaataaat cattatctgg gaaattcgaa gcaaaaacat ttcgcgcgcc ttcaaaaaca
     5041 aacgccgcat ccatttgctt gttcaacact gcggtaatct cactcggaat atcattcaca
     5101 gtggtcatct ttgtatctgc agtaggatca atcccagcat ctttcaattc agcgacagga
     5161 tagatgtagc cgctagcaga gttaggactt aaggttgcga tattttttcc ttttaaatca
     5221 gttaacttat tcaatccgct atcggcacga accaatactt ctcctttaaa ggtgttcgta
     5281 agtttgtcct ttaatggcaa gccagtttct tgatcataat cgccgagctg agaagtcagg
     5341 attgcatctg cagctccagc atcctttgct tgaacatagg ctgcaggcgg cataattccg
     5401 acatcgactt gaccagaaga catcgcttcg acgatcgttg aataatcagt agctaacgtc
     5461 acttctacct cacgatctaa ctttttcgat aaatacttag caaatggttt tgcttttgcc
     5521 tccatactac catcattgtt tgttggaacg aattgaacaa ctaatttttc tttgctctct
     5581 ttttctgagc tgctacttgt attcccagaa ccgcaggccg ccaaagtggt caataacatg
     5641 actccgatga acccttttgc taactgtttg aactgcattt ttctcctcct gttggatata
     5701 atttctaata cgtctaaatc atagcaggga atcaaaatca atgaagtgac gcacgtccta
     5761 ttttacacaa cttttacata caatttacaa acaattataa ataattattc gtattgagcc
     5821 gaaaaacaat ctaagctata ataaagaaaa acttcttaga ggtgacgatt tcatgaaaaa
     5881 aatccatgat acgaagcaat taactgctta cgttgagaag cacaacctgc aggattatat
     5941 ggattcagat atattggcaa tggcttcttt actttttttt gaaaaggaag agcacctcat
     6001 tcaaacggga aaaacctcag attatttgta ttttttagtg gatgggacgg taatggtgta
     6061 ctcgtattct tctgatacac aaaatatttg catcgattac gcaaagcccg ctacaccact
     6121 cggcgaagcc tcttcattgt gggaattatc gccgaaaagc agcgttaaag cagtgactaa
     6181 gtgcatctgt atcagcattc cgctaaatca atatcgaaca cagcttcaaa aagatgttcg
     6241 ctttttgcag aatatttgcc agttactaag ttatcgttta aattccggga taaatttggc
     6301 gaactcactg acagaacctg ttgaaactcg tttagcaaag tttattttga cccatcacga
     6361 aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca atcttaaatg tcagttatcg
     6421 acatctttta cgaacaatca cgaattttcg tgaggagaat attatcgaaa agaaaaagaa
     6481 ttattacttg attcatgata tcgaagcttt agaggactta gctgagaata taagtagtcc
     6541 agtaaaagca gaataacagc tataaaaaaa cagcagtcag aagtgattgc tggttttttg
     6601 atagtggaga aattgggaaa cggaaaatct tcgttgaaga gtagtgttct atatattggt
     6661 atgctatact tttaaaagtt caatgtgtca agacacataa aaaggggaga aaaattttga
     6721 agaaaatact tgtttcattg ttgtttttgg tgtcattagt aacgttagtc ggctgtagtc
     6781 agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa aatcgctttg gttct
//
LOCUS       DACEPW010000130         6640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEPW010000130 DACEPW010000000
VERSION     DACEPW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.132"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(89..1207)
                     /locus_tag="IW819_002071"
     CDS             complement(89..1207)
                     /locus_tag="IW819_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245166.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1678..2985
                     /locus_tag="IW819_002072"
     CDS             1678..2985
                     /locus_tag="IW819_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1245167.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            3104..4303
                     /locus_tag="IW819_002073"
     CDS             3104..4303
                     /locus_tag="IW819_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1245168.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4330..5598)
                     /locus_tag="IW819_002074"
     CDS             complement(4330..5598)
                     /locus_tag="IW819_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1245169.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(5761..6408)
                     /locus_tag="IW819_002075"
     CDS             complement(5761..6408)
                     /locus_tag="IW819_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ1245170.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT         2005 a         1393 c         1178 g         2064 t
ORIGIN      
        1 caatctcttc ttatttcttg aagctgacca cttctgtcac aacctcttcc ttaatactca
       61 ggtcaaaaca ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt
      121 catctggcgc atctttttga tccaaaataa acactttccc gccattaatg atcacatcat
      181 ctgctaatgc attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac
      241 catcagctaa gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg
      301 gaacgatatc aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg
      361 attcttgagc tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg
      421 atagctgcgc aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa
      481 tttttttgaa catcgtctga ttttctggta atgcaaataa ataaatcggc cattgttcag
      541 gattttcgaa agaatccttt aagtacgtat caacagcttg gtaatagttg acccaatcga
      601 tctctacttc ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc
      661 ctcctccatt agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa
      721 cgacatcaac cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgacct
      781 catacaattt catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc
      841 catttttgat aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg
      901 ggatactcaa acgatggata taggtctggt cagcagttat aatgaccgct acactcgtag
      961 ttgcactacg ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacggag
     1021 accagtcatg ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt
     1081 tgaatttgat ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg
     1141 tgacgaatgg tcctttagcg ccttctgaag tcaattgtgt caataattct ttatcctttt
     1201 ttgccataaa cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca
     1261 caacactatt tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt
     1321 taaatttagc tattttttta agttatcttc ataatttcct caattattta gccccagtct
     1381 tcaactattc ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta
     1441 tgcatttaat tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt
     1501 cccgcttatt agatgcggaa acacggccta ttcctgtaaa agtacgtaaa aacgaccgct
     1561 aaacttccat aacgcgcgtt cttatcctaa agttttttca gaaacattga aactctctcc
     1621 cctctctgat aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg
     1681 tccataaaaa aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct
     1741 tttctaccag ctgttgctgt gcaagctgaa gatacgttca aagtaaatgc aaaagcagct
     1801 tttgctgtag atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg
     1861 gggatcgctt caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa
     1921 ggaaaactgt catgggatga taaagtttcg atttcagatt atgcagaaaa tttgagtatc
     1981 accccagatt tatcgaatgt tcctttgcat aaagaaaaca cctacactgt caaagaatta
     2041 ttcgactctg caatcatcca gtcagccaat gcttcaatgg tggccttggc agaaaaaatc
     2101 tctggcagcg aagctaaatt cactgaacga atgaaagaac aattgaaaga ttggggaatc
     2161 aaagatgcaa caatcgttaa tgcatcagga ttgaacaact cttatcttgg cgaaaaccga
     2221 ccagaaggca ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggccatcgtt
     2281 gcccgccatt tgatcttgga tttcccagaa atactggatg tatcaagtac tacgacccaa
     2341 atgtttggcg aaaatacgca atcaccagtg gaaatggtca actggaactg gatgctccca
     2401 ggattcgtta attacaagga aggcgtggac ggattaaaaa ctggtacgac tgagcttgca
     2461 ggtgcttgtt tcgttggtac gatcactaaa aacgatcaac ggatcatcac tgtcgtattg
     2521 aatgcggacg gccatgcgga aaacccgagt gcccgcttca atgaaacaga ccgtttgatg
     2581 gactactgct atgacaattg gtcagaaaaa gagctcggaa aagctaacgc aagtattcct
     2641 agtctaaaaa ccattgatgt aaaagacgga aaagaatcaa cagttcctgt tgtgcttaaa
     2701 agccctgtaa aagtatgggt acgaaacgat atggatactg gcaaattaac gatcacgcca
     2761 acacttgaca agaagcaagt cactgacaat gagatccaag cacctatgga taaaggtaca
     2821 aaaatcggta ccgctacgat caccttggca gacgataaat taggctattt agaagacaac
     2881 aatgcaccat caacagagat cattacggat aaaacagtgg aaaaagcgaa tatattcgtg
     2941 attggctggc gacgcgtcgc agaatttttc gacaacttgt tttaattgaa caataagaaa
     3001 aacatctttc catcggaaag atgttttttt gctttttcta gtttaaataa cagctttttt
     3061 catctatttt cgttatactg tataagaaaa aggagttgaa tttatgaatc taaccagaga
     3121 tctgatcttt ttagttctct atcttattgg ggtattctct atcgtttatt ggcgaaaaca
     3181 gaagaaggat ccttccttgt tcttttacgg cagtacgtgg acatttacgg taatcttaac
     3241 ttattttgtc gtgttggaaa ttttttattc gaccttttat tttttgattc cattgttgtt
     3301 ctttagtatc tttgcttttt cttatttcac agaaaaacga aggctgctga atggtctttt
     3361 attcaatgtt ttcctcatca gtttcggcat ctacttgttt gtcttgctgt atgaaacgca
     3421 aaatattttc cttggcggac tgatcgcctt gatcacgatt ccattgctac ttgtcctttt
     3481 atttggtatt tatggattga ttgtttttct tttttggaac ggggtcacag ttttacgtag
     3541 agaatcgcat tcattagcta atcttttaac gttgatacta gccatttttc ttacactgtt
     3601 tcttgttttt gactttttct tgttaaaata tttgccgcaa tggataaatg ctttgttttt
     3661 ctgcgttccc ctgatcttga tctatctatt tatcgtattc tacaactttt tgactgtctc
     3721 attcttgtat cagttcaacc ggcctcgata caatcaagac ttcatcgttg tgttaggcgc
     3781 tgggttgatc aatggagaaa cagtttctcc tcttctagcc aagcgaatca ataaggcaat
     3841 tgctttttat cgggctcaaa gccgtgctac attaaatccg cccatcttat tgatgtctgg
     3901 cgggcaagga gcagacgaaa aagtaccaga ggccattgcg atgaaacaat atgccatgga
     3961 acaaggaatc cctgaaagag acattcttgt cgaaaccaat tcgacaacca ctttggagaa
     4021 catgctctat tcaaaggaaa tcatggatca gcaaatgaaa gggccatacc gcgcgatctt
     4081 tagttccaac aattaccaca tttttagagc cggtctttat gcgtggcaag ccaaattgaa
     4141 agcaaatggt attggtgcaa aaacagcttt ctactacttg cctaatgcgt ttttaagaga
     4201 atacatcgcc atcttgatga tgaacaaaaa aagacatatg atcatttgcg gattgctttt
     4261 cgcaggctcc gcattcttat cgctgattac tttacttttc taaaaaaaca aggtgtgaca
     4321 aaacatatgt cacaccttgt ttttttcttt cattctctta actattggca aaaagagtat
     4381 cccttttttg accgctactt gctgagcaga ataaataccg ctgttgcctg aacacataaa
     4441 gctgatcaag cagatcataa agaagtatac tgccgcttgt gaaccgaaca attcgatccc
     4501 catcacaaag caggcaatcg gtgtatttgt cgcaccagaa aatactccga taaagcccaa
     4561 tcctgcaaga aaaggaacag aaacatgaag caaaggcgct agtgctgcac caagcgttgc
     4621 accaatttca aacaatggtg tcacttctcc cccttgatat cccgcgccta aagacaaaac
     4681 tgtgaagaat aacttcccaa taaaatcata gacatgtgcc tgtccattaa atgattcttg
     4741 aagtaacgga agactcaatc cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac
     4801 aactacagct ccacctatcg tatttcgcca aacaggatga ggaatccatt gtgcatatcg
     4861 cgcttttaaa aagacgatcg aacggctgaa tacccaacca atcaaaccaa aagcaatccc
     4921 agcaacaaaa actttgaaga acagcaacat actccattca ggaatcacac ccatttggta
     4981 atgtagatga gaaacaccat agctttctgt catgaaattt gcaaacagtg cagcaaagaa
     5041 gctagggaag atcgcttctg ttcggacttt cccgattgcc aacacttcta atccaaataa
     5101 tgttccagct aaaggtgtgc caaatacaga actaaaaccc gcactgattc cactgataat
     5161 caagatttcc cgttctgctt tatccaatcg gaacaaacgt gctgtattat ctgctaacga
     5221 tccccccatt tggactgctg ttccttcacg gccaacagac cccccaaata aatgggtagt
     5281 tatggttcca aacagtgtaa gtgtaataag ccgcaacggg attttttctt ctccaccatt
     5341 tccttgatcg atcactaagt tgtttccacg actagaaaga ccgccgtgat acgcatagag
     5401 ataagcaaaa agcgcaccac taactggtag tagataaagg agccaaggat gtgatagtcg
     5461 gatatctgtc acaactgtca ggctatttaa gaaaaaagca gacaagctcc ccatgaatat
     5521 accgagaatc gctgtgatca ccagccactt cagcatgtac actaaaatgc gcattggact
     5581 cggtaattgt ttttccataa aaaattcctc ctcaataaaa aaacgcctac taaagaaagg
     5641 ggctgtcccc agactttagt aggcgtcatt agctatcttt cgttagcatt taaaggcgaa
     5701 caccatcacc tattcacttc cattaaaaga tagcatattt ttctgtaaaa aacaataaaa
     5761 ttatcgttct ttctttacac gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg
     5821 ttgattacat tggtcactat catgacaaat ataattcccc tttttcgtat acgtgccatc
     5881 agaacctgct ttcgttgtag aaagaaaaag cgacacattc gatgttttct gacagattgc
     5941 acagattccc ttatttatcg ttggtgataa agtaccgcta atccctttta atttttcatc
     6001 ttcgtaatag accatgaatt ttttctgcgt acctggatca ttccagccga tgtacgtatg
     6061 ttcttgcaag tcgatttcat tccaagctgg ttgtttgagt ttttttactt ttcggaataa
     6121 tttctcgatt tgtggtttgc taggctgcat aaacggctga acgatttgct tcagcttctc
     6181 caatgctctt tctgcttgcg cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt
     6241 atcgatttct ggcagttcgt gatttaatat gtctcgaatc ttttcagctg ctagtgcctg
     6301 aactgtttgt accgtttttg gatcattgac agatgagtac gcatttaata actgttctac
     6361 ttctcttttt ataaaaaaat attggtatgg ttttattgtt tgttccatga ttttaaagtc
     6421 ctttcttcta accaaagtgc atactttgta gaaaaaagtg atcttctgca aagtatgtgt
     6481 tacctgaaga tcccggactc ttttaaataa gatacgtttt gcatcttatc atcaccccct
     6541 ttaatgctta ttcttctatg tacatgattc attcgataaa taaaagtaaa ccgtatcttg
     6601 aacagatagt caactgcaaa cagaaaaaag gactgtgaca
//
LOCUS       DACEPW010000131         6601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEPW010000131 DACEPW010000000
VERSION     DACEPW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.133"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            132..512
                     /locus_tag="IW819_002076"
     CDS             132..512
                     /locus_tag="IW819_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1245171.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            608..1117
                     /locus_tag="IW819_002077"
     CDS             608..1117
                     /locus_tag="IW819_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245172.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            1194..1373
                     /locus_tag="IW819_002078"
     CDS             1194..1373
                     /locus_tag="IW819_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245173.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            1428..1991
                     /locus_tag="IW819_002079"
     CDS             1428..1991
                     /locus_tag="IW819_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1245174.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(2038..2553)
                     /locus_tag="IW819_002080"
     CDS             complement(2038..2553)
                     /locus_tag="IW819_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245175.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(2579..3448)
                     /locus_tag="IW819_002081"
     CDS             complement(2579..3448)
                     /locus_tag="IW819_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1245176.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            3574..4254
                     /locus_tag="IW819_002082"
     CDS             3574..4254
                     /locus_tag="IW819_002082"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1245177.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4407..5387
                     /gene="pta"
                     /locus_tag="IW819_002083"
     CDS             4407..5387
                     /gene="pta"
                     /locus_tag="IW819_002083"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1245178.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            5510..5983
                     /gene="tsaE"
                     /locus_tag="IW819_002084"
     CDS             5510..5983
                     /gene="tsaE"
                     /locus_tag="IW819_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1245179.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            5976..6500
                     /locus_tag="IW819_002085"
     CDS             5976..6500
                     /locus_tag="IW819_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1245180.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
BASE COUNT         2184 a         1171 c         1391 g         1855 t
ORIGIN      
        1 acctactttc tgtttttttg ccttttttct tataaaaagg aaaaacagct atttttttca
       61 atctgacaca tttacaatgg aactggagat taatatagat ccgagcttaa acgaagaata
      121 ggagtgctca tatgaacctt acaacgatcc atcatatagc catcattgtt tcagattatc
      181 agaaatcacg tgatttttat gttaataaat taggatttga agtaatcaga gaaaattacc
      241 gcaaagaacg agatgactat aaattagatc tgaaactagg aagtgctgaa ttggagattt
      301 ttggaattgc taatgcgcca caacgaccaa actatccaga agcttgtggc ttgcgtcatc
      361 ttgctttcaa agtagacgat attgaagaaa caattgcaga attgcagtcc ttagggatcg
      421 aatcagaacc gattagagaa gataccttta caggaaaaaa aatgacgttt ttctttgatc
      481 cagacggatt gccattagaa ctgcacgaat aaagataaca aaattgtcac ttttttttcg
      541 tttcattcga tttttcattc gttcatttct tgatattctt taaaaaaaag aaacaggaga
      601 atgaagaatg aaaaaaatga tgtttggtgt tgctgccctc atcgtaatag ggattaccgg
      661 tatgacggga ctgctcaatg ccgcttttca catggatgat attagtgacc aggaatatcg
      721 gatctcattg aaaaaagcag cagcaatcgt aaaaaacgaa acaaaagaac ataacatatc
      781 tgctattcat tttgcagttg aaaaagattt ggagaatcct acgaatgcaa catatgaata
      841 ccagtttttt tgtgaaggac aagtcatagc agtcaatcca tccactggga aaacaaccgt
      901 acgaaatgac acggaaaagg ccgcagaaca tctttttgat atagaaaaaa cggaagagtt
      961 caaacaaccg caagcagcga tgaaggaagc agtcagctac tacgggaaaa aagcaaaagt
     1021 gaaagagtgg acacttctag tcaaagatga acgtccttat tataaagtcc gattaataga
     1081 tggagaacag gaccgtcagg tatggatatc tgcatagaag aaggatattg cttctctttt
     1141 ttacaatata agagtaagct aagagagtaa agatgaaagg aagttgagat aacatggaaa
     1201 aaatgaaatg ccctaattgc ggaaaaaaat ttgcatacga agaagtcaat aatgtagtcg
     1261 aacataatga taaagaaatg ccagtcgtct gcccttattg ccgaactgaa gcggcaagaa
     1321 tcgttactca tggttatttc attactgaaa agattgaaga ttttttaaaa taacgatttt
     1381 gcgatttctt tttctgcatg ttagaatgga gaaaaggaga ggaaactatg attcgttttg
     1441 cgagaaaaga agatgctcca gaaatcgccc cattgatctt agtgattcta aaagatatgg
     1501 aactgccatt tttgaagcag acaggggaac aaaagacttt agagatttta gaagaagcaa
     1561 tcactgatcc aaattatcgt tacagttatt cacgaggtat cgtagaagaa atagacggta
     1621 aagttgcagg agttgcttac ggttatacag atgaagaaga atcaatcatc gatcagccac
     1681 tggtacctat cttagagaaa tatcagctag atgcgtctac acgtttgttc attgataaag
     1741 aaacgtttcc caacgaatgg tatttggact ctatcagtgt atcagaagat tttagaggac
     1801 aaggcatcgg ttcgcgatta ttagaagcat tacctaaatt ggcgaagaaa gctaatcgct
     1861 ccgtcatcgg tctaagtgtg gatgaaaaaa atccaaaggc taaaaaatta tacgaacgcc
     1921 atgacttcaa agttgtcggt cagcggatga tcagtggtca tctttatgat catatgcaaa
     1981 agaatatcta aaaagatgta cctgaaatgc gtatttccgc atttcaggta catcttttta
     2041 acttaagtta agtcggtaag aaacaggctt atcgcgactt ttgtctttac ggtagagcca
     2101 gtaatctaaa accgcagcaa tacaaataca aactggagca tcttcatcat ttgagacatc
     2161 caatacatag taatctcctg aaaaaagtgt tgctttatcc attttcatga tttgctgatt
     2221 gaaatgatgg atttgatatt gatgattata gatatcacct tggactgtcc agtgaagtct
     2281 acgaatataa taaaagtcac cgggccaatt gaatattttc tgcatggttc ccaccttttc
     2341 gaactcttta tacagctcaa aacgcgtacc gaatgtcagg cttatctgct tgatgtgagc
     2401 aacgagatcg ccattcatgg cataaagaga caatgcatcg ccctttgttc cccagcgacc
     2461 aaccatcaaa aacagggaat gtcctttttc gtttttgaca attgtccgag ttgcacgtcc
     2521 aagctgcttt tcctgaatga aaaattctga catctatatc cctcttttct agataaaact
     2581 acaagtcttc tgcaatcgcg cgcaatatgg cttttcctac gccagattca aggtttgtgt
     2641 ccgttgtata tttggcataa tcttttactt cgatttctgc atttcccatt gcgaaactga
     2701 ctcctgctac ttgcagcatg ctgacatcat tgaagttatc gccaatcgtc atgacttctt
     2761 ctaaagaaat cccacgctcg tgagcgacat gggcaacagc aattcctttt tgagcgtttt
     2821 tatggttcac ttcgatattg ttttgaccag aggaagtaac ggctagatcg cctaaaagag
     2881 aaatctgctt tccaactgga cctaatacag ctggcccttc tgtatgaaaa caaataattt
     2941 ttaaaacttc cagattcttt tcttccaaaa tagcacgaat actgtccacg tatgtgatgt
     3001 gcaataactc cagcttagct gaggccatgg caatcgccat tttatacgta aggtgaggta
     3061 aatgggtagc caccatggaa gcaaaacttt caattcgttt cgcttgattt tcagaataaa
     3121 gaccatcgtt ggttgctact tcatagtaaa taccgttagc atctagaata tctaaaacag
     3181 agattgcttg attatttgga atcgacacgg taaacaatga atttccttct ttatcaaata
     3241 cctgtgcccc gttcaaagtg atcatcgcac aatcgattcc tacctcatct aaagctgcgc
     3301 gagcttcttg atagtttcga ccagtagcga ccatgaactc aacaccggca tcatttgcaa
     3361 aacgtatcgc ctcggcattt tcaggagaaa cactcatatg ggcatctaat aatgtgccgt
     3421 ccatatctga tgcaattaat ttgatcataa tgattatcgt tcctttctgc ttcttgttcc
     3481 tagtttacca tattaatcaa gtgctgaggg tgaagattgt cccttgtgat tgaagaaaat
     3541 gtgagttatt ctttttatta ggaggtggag tttttgaaag aaatcattca taacagttgg
     3601 caagaagttt tgtcctcaga attttcaaaa gattattatc tgcatctaag agaattttta
     3661 aaaaaagaat atgcgtccca aaagatccat ccagatatgt atcatatcta tgaagcattg
     3721 gaattgactc cttatgaaga agttaaagtg gtgattttag gacaagatcc ttatcatgga
     3781 gaaaatcaag ctcatggctt atctttttct gtgcaacctg gcgtaaagat cccgccatcg
     3841 ttaagaaata tctataaaga gctttatgat gatcttggaa tcgcacctgt tcaacatgga
     3901 aatctagtgt catgggctaa acagggagtc cttttactca acaccgttct taccgttcgg
     3961 gaagggcaag cgtattctca tcgcgggaaa ggttgggaaa gattaacaga cacgatcatt
     4021 gaaaaattaa atgaacggga aaaaccaatc gtgtttatct tgtggggaaa accggcacaa
     4081 gaaaaaatca aaatgatcga taagagtcgc catatcatca taacatctcc acatcccagt
     4141 ccgttatctg caagtcgtgg cttttttggt tccaaaccct tctcgaaaac gaatgatgcg
     4201 ctccttgcgt taggagaaga accgattgat tggcaactgc ctgaaacagt gtaataaaac
     4261 agcgctttcc tgttatagtg cagttcgctt tgtatgtgaa aaacttaatt tttccagcat
     4321 ttttgactga tacagataaa ataaactgtt ctttttttct ggaatagtgt atgataatac
     4381 atataaaaac gtctggaggt catcttgtgg aattgtttga tagtttaaga tttaaaattg
     4441 ttcgccgtgg gatcaaaatc gttttcccgg aagctactga tgctcgtatt ttaggcgcag
     4501 ctgcccggtt gaaagcagaa gaacttgtgg aacccatttt agtagggaac gaagaagaaa
     4561 taagaaatgc agcgcatgcc agaggaatca aaacatctag ctttacgatc atcagccctg
     4621 ataattacga taagtgggat gaaatggtgg atacttttgt tgaacgtaga aatggaaagg
     4681 catccaaaga acaagcagaa aagattttaa aagatgtgaa ttactttggt acgatgctta
     4741 cgtatatggg gattgcagac ggaatggtct ctggagcgat ccattcgact ggcgacactg
     4801 ttcgcccagc acttcagatc atcaaaacaa agcctggcat cagtcggaca agcggagcga
     4861 tgatcatggt acgtggaaga gatcaagaaa aatatgtctt ttcggattgt gcgatcaacg
     4921 tgaatccaac tgcgcaagaa ttggctgaaa tcgccgtaga ttctgcaaaa acagcagaat
     4981 tgttcgatat cgaaccaaaa gtagcaatga tgagcttctc gacaaaaggc tcagcgaaat
     5041 cgccagaagt agacaaagta gttgaagcaa ctaaaatcgc taaaaaacta gcgccacaat
     5101 acatgatcga cggcgaattg caatttgatg cttcttatgt tccttcagtt gcacaactga
     5161 aagcgccaga ttcagaagta gctggtcaag caacagtctt tgtatttcca gaattgcagt
     5221 caggaaatat cggctataaa attgctcagc gtttcggtaa ctttgaagca atcggaccaa
     5281 ttctacaagg cttgaacaaa ccggtttctg atttgtctcg tggagcaaat gaagaagatg
     5341 tatataaatt atcgattatc acagcagcac aaacattgat tgattaacaa atacacgcga
     5401 actagccaaa aacagcagct gctgttcttg actagttcat tttttcttta aaaaaacttc
     5461 attacgcgat atactggcaa aaagaaagaa gagcgggaga agatgaaaaa tgatcgaact
     5521 atcaggatta acaatgacag aacaattcgc agaggcaatt ggtcattccg caatgccagg
     5581 tgacaatctg atattgactg gtgatctagg tgcgggtaaa acaacgttga cgaaaggaat
     5641 tgcccaagga ttagggataa cgcaaatgat caaaagtccg acatacacga ttattagaga
     5701 atatagtcaa ggtcggattc cgttgtatca tatggatatt tatcgggtag cagctagcgg
     5761 agcagattta gggttagatg aatattttga aggtgatggg ctttccgttg ttgaatgggg
     5821 aaatctatta gaagaagcac tgccggagga ttatttggaa ctgattttag aaaaaagtga
     5881 tacagattta gaaaagcggt atgtgaaact aatctcatac ggggagcaaa gtgaaacctt
     5941 caaacagcga attctagaaa aatgggctga aaatcatgag tgaagaagtt gggatcatct
     6001 tgcgggaagc agtgccagga gatgcaaaag acattctttt gatgatggga caagttaata
     6061 aggagacaga atttttagtt ttggatgaag cagaactcct gttaccacca gaaaccttag
     6121 aagaagagtt ggattatata tacgaaagca ataataattt attgctgctt gcaatctatg
     6181 aagggacgat tattggaaca gcatctgtca aggcagacag tcaatttcgt ttgtctcacg
     6241 tgggagaagt cggtatcagt attctgcagg aatattgggg aatgggacta ggcactctta
     6301 tgctagaaga aatcatcagt tgggcgaagg aaatgggtgt actttttcga ttggagcttg
     6361 acgttcaagt tcgtaatgaa agagccgtac acctttaccg aaaaatgggc ttccaaatcg
     6421 aagcggtcat gcctagaggg gcaagaacag atctaggtga atttttagat gtatataaaa
     6481 tgagttattt gattgaatag aagaaatagg aaaagagtga gaaactgtca tccttttgag
     6541 aaggcagaaa aacacacgaa gggactgtga caaaagtcgt gtcgcagtcc attttttttg
     6601 a
//
LOCUS       DACEPW010000132         6478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEPW010000132 DACEPW010000000
VERSION     DACEPW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.134"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(177..1595)
                     /gene="nhaC"
                     /locus_tag="IW819_002086"
     CDS             complement(177..1595)
                     /gene="nhaC"
                     /locus_tag="IW819_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1245181.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1998..3221)
                     /locus_tag="IW819_002087"
     CDS             complement(1998..3221)
                     /locus_tag="IW819_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1245182.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3252..4091)
                     /locus_tag="IW819_002088"
     CDS             complement(3252..4091)
                     /locus_tag="IW819_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1245183.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4108..4788)
                     /locus_tag="IW819_002089"
     CDS             complement(4108..4788)
                     /locus_tag="IW819_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1245184.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4790..5863)
                     /locus_tag="IW819_002090"
     CDS             complement(4790..5863)
                     /locus_tag="IW819_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1245185.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
BASE COUNT         1868 a         1410 c         1104 g         2096 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa
       61 tggctttcca catttttgta taaatttggc ttaataccga gaaactggct tttgaatacc
      121 gtttattcgt aaaagggatt gtgacaaaag tcttgtcaca atcccttttt atgtttttat
      181 tgttctaatt gcgctgtctt gcttactgct tcttgcggtt tcacaaattt ttttgagatt
      241 ccccaacctg tgaaggcact gaccatcgaa aacacaggag aaagcaaact gaaaaatacg
      301 aatggcaagt aagtgagtgt aggaatacca aatgtacttg ctacgaaact tccggcaatt
      361 ccccaaggca ctaagtagtt gattactgtt ccaccatctt ctagcgttct acctaataca
      421 gttggatcaa atcctgcttt ttcataagtt tctttaaatg cattaccagg cagaataaca
      481 gataggaatt gttcaccaat aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa
      541 atcaaatttg gtgtgttcgt catctttctt gatactcgtt ccatcacggt tgagatcaag
      601 ccaaattcga tcaacaatcc acctaatgac aaagttagaa taatcaatga cacagtaggc
      661 atcatacttt cgattcctcc gcggctaagc agtgaatcga tttgttggtt tccagtattt
      721 gaaacaaacc ctgattcgat gaccccagcc aacttcgtta aggaagtttg tgggttttga
      781 ataaaaatca ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc
      841 tttttccatg cacacagtaa cattaaaaat aaaggaatca gtgaccagat tgaaatagta
      901 aagtttgctt ctagaacatc agtcacttca cgaatagatg aaagacttgc ggctttactt
      961 gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac agttgaccac
     1021 atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt
     1081 gattcggata atggagacat cttatctcca aaaacggcac ctgaaacaat agctccaaca
     1141 accagcgctg gatttaggcc tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt
     1201 gacataacag taaaggcact ccctacagcg ctacctacga ttgcacacac aacaaataca
     1261 gaagggacaa accactggac actgatcaat ttgaacccaa tgaccattag agttggaatg
     1321 atccctgctt gtatccatac agcaatcaat gaacctacta aaataaaaat aaagattggg
     1381 atgattcctg gtttgatgcc attgatgatt ccttcgtgca gccaatctgt cgggtatctt
     1441 tttatcactg catacaacat gaccattccg atcgctgcta agatcgtgat attaggtgag
     1501 acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt
     1561 gcttctaaaa aacttggtgt tccttttttt cccatgataa tctctccttt tttagttgtt
     1621 cttatgttct aaaaaagtta agatatcgga ttactccgcc gcaattcatc cgctattcct
     1681 cctttataaa aataaaaaga cctgttttaa actaaggacg aatgactccg cggtaccacc
     1741 ttaattaaaa acagatctgt ttttcacttt caaactttta ttcaaatgta tggactttcc
     1801 atgtaattcg tcaattaggc ttgatcggtt cacagcgacc accgactttc tgaacaaaac
     1861 acctaaccgc tactgcgtga aaagttgact tgtatgaagt ttaccttcca taagatcatt
     1921 tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa ttatttttat aactttcaca
     1981 cacaagttta ttttttacta gaaagtagca ccctcttgtt tttttcttgc tactatttct
     2041 tgatgttctt gcttgattct ttctaatagc tttggctctt tcaacagatc aagagctgta
     2101 ttggcgagta gttctgctgc aatcgcaata gaagcaaggc ctttgtcact tttagcagca
     2161 gctttgaatt cttctgtgtg tccggcaata tatgtctcag agattgctac cgtaggctgg
     2221 attgttggta tgacttggct gacatttcct acatcagaag aaccgagact tgttctttgt
     2281 tccgtttcta tttcttcatc tggaacacct gcttcttttg catgctggaa gaataagtca
     2341 tcaaaagaag gagtaacgat gacatcatca actgcatttt ggaacaatcc aaactcaaaa
     2401 gtcgtgcccg tacttatcgc agcacctcgt acgatatttt ccactttttc atatacttta
     2461 tctaatgttt gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg
     2521 acgttggcag ctactcctcc gtctgtaatg atcccatgga tattgacatc ttttggcaat
     2581 tgcaagcgta gagcgttgat tccattaaac acttggatca atgcttccaa agcatttact
     2641 cctttttcag gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga
     2701 tcattcgcta aagattctgt cgttttccca taacgaaaag ctggatggac gcataacgct
     2761 acatcgacat cctcaaaaaa accttctcga acaaagctgc ctttagctga tccgttctcg
     2821 cccccttctt cccctggggt gccatataca cggatttctc caccagtttc atcaataaat
     2881 tgctccacta tgctggcggc taatacggag taagtaccaa acaaattatg cccgcatgca
     2941 tgaccaatac ctggtaaagc atcgaattct gccaaaaagg caattgtggg tccgggttta
     3001 ttggaaatat aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt ttctacccaa
     3061 aacccttctt tttctaattg tttaatcaac gctgctgagg aataaaactc atagttactt
     3121 acttcaggat gttcgtggat atctagagca agtgcttgat attctggtaa acgatattgg
     3181 attagatcct tcaattggtt tgttgagatt gttgttgtca taaaaaattc ctcctatgct
     3241 agtcattttt cttattttct cccgaatgtt tcccatgcag gcacgcttga tccttttgat
     3301 gttttctcga ttactttgac tgtgtcttct gtttgataag cgtctactat tttttgatac
     3361 gttttatttt tttcatcttc ttttcttgca acgatgatat tcacataagg acgtgcatgg
     3421 tcatccactg gttctaagaa gatggcatca gaagcaggat taaatccagc atctacagct
     3481 acaccgctat tgatcactga aatgtctaca tctgacaaag cacgtgctgt ttgggaagca
     3541 tctaattcag aaatgtctag attttttcta ttctcggtaa tatcagaaac agtgggcgct
     3601 tgtccttttg ccttatccac tttgatcaat ccagcagttt gcaataacag aagagctctg
     3661 ccgccatttg taacatcatt agggattgct acttttgctc cgtctttgat atcagctaca
     3721 tccttcaaag tgttagaata aattcccagc ggtgcattga cagtatttcc gatcgatact
     3781 aaatcagttc cgtgttcttt attgtaattg tctaaaaaga attgatgttg gaaagaattc
     3841 aaatcaatcg atccatctgc taaagccgca tttggttgat tgtaatctgt aaatttcacg
     3901 tattccaaat taatgttctc ctttttcaaa cggtctttca cgttgtccca tacatctgta
     3961 tcttctccaa taattcctag ttttacggta gtttcttttt tgtcagttgt atctgctttt
     4021 ccttggctac accctgccaa ccaaataatt gctacaaatg taatgactac aaaacgaata
     4081 atttttttca ttttttctcc tccactttca atgtgtaatt tttttaatca gttgattact
     4141 gataaattga cttaaaaaaa ctaagattag aatcaataat gtagccacaa tcgttacatc
     4201 tgtttggaat cgattatacc ctctcgatat agcgagattc cctaatccgc ctgcacctac
     4261 tgctccagcc atggtagtca gtccgatcac gttgatgata gtcaacgccg agacccgaat
     4321 gataccaggc aaaccttcta acaaatacac acgtaaaata ataccgattg gactggttcc
     4381 catcgcttct gccgcttcga tcaccccagg atcaacttct agcagtgcgt tctcgatttg
     4441 acgagcaaaa aacggaacaa ctccaaaaat caatggcact aaagctgctt tcgcaccaat
     4501 cgtcgttcca actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg
     4561 gatggaacga atgatattga tcagcttatc aagtatttca aatagcagac ggttagctag
     4621 taatccatta gggcgtgtca ctactaacgt cacacctaag atgattccga tcatcccgcc
     4681 aatgaatgct gtccaaaaag acatatatag tgtttgcttc gtcgcttcga caaattcatc
     4741 cggaatcaaa taaacattgg gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc
     4801 cttcgatcac tttgatacga ctatctaatt cgtttttttg taaaatattt atttttacac
     4861 cagatttgat caaataatcc aatgcttttt gccgctgttt ggtttctcca gctaaagtaa
     4921 cgatcaaact ccctaacggc gtttcttgta ataactctac attgccatat aaaatattcg
     4981 ctgttacttg atatttgctg tacagatgag cgattagagg ttcgttcgtc tgatttccaa
     5041 tataacttaa ttccaccagc catttattgg cgatctgatc ggcaaaagca gaatgggcaa
     5101 tgatcgtttc taatgcttgg tcaagatgag tagctgttcg aataaaatct ttggtcaatt
     5161 cttcttcagg attgctgaag atctggatgc tactaccttt ttcaatgatt cttccatctt
     5221 ccataaccgc taccttgttg cagatttctt tgacaacttg catctcatga gtgatcagga
     5281 cgattgttaa acctaactgg cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg
     5341 ttttcggatc aagtgcactg gttgcttcat cacataataa taccttaggt tcattcgcca
     5401 aagctcgtgc gattgctact cgttgttttt gtccccccga cagttgttta ggataagcag
     5461 atgctttttc ttccaaaccg acaagttcca gtagttcatt cactttttga cgacgttcct
     5521 gtttcgattt tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt
     5581 ccattagatt gaaatgctga aagatcatac cgatggcctt tctttctttt cgcaattctt
     5641 tgggagagag ctgactcaaa ttcgtctgat tgacaatgac ttctcctgct gttggtttct
     5701 gcagcaaatt catcacacga actaacgtac ttttccctgc acctgagtaa ccgacgattc
     5761 cataaacatc gcctttttca atcgttaaat ctacagaatc tactgcctgt atctcttttt
     5821 ctttttgttt gaaacataca gaaacatctc gcaattcaat cattaaaatt cctccttatc
     5881 taaaaaaagc gtcctcaaga aaatgacatt ttcattttct tgaggacgct tttttgcgtg
     5941 ttaccacctc atgttcaagc tgtgttcaca cacaactcct cttcaagtac gatcatactc
     6001 tagcaagata tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat gaattgctcg
     6061 atggccattt tccaatagtc gttccttgct tcttctcagc taccgaagct ctctgtcaaa
     6121 ggttcttcta tcgtacttac catttcaatg caaataatat tcaaaaaaaa atccttttac
     6181 taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt ttcggaagtt
     6241 tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta acggtcgaac
     6301 agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc agcttttctt
     6361 acttgactct tcacaccagc cagagcctct ctgcaaagtt tccaagctta ctttccttct
     6421 caacgaaatc aatattttgt tgttgggaat aatctacagg aggaaaactg aaaagtca
//
LOCUS       DACEPW010000133         6467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEPW010000133 DACEPW010000000
VERSION     DACEPW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.135"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(109..564)
                     /locus_tag="IW819_002091"
     CDS             complement(109..564)
                     /locus_tag="IW819_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245186.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IW819_002092"
     CDS             complement(718..1926)
                     /locus_tag="IW819_002092"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1245187.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLVEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IW819_002093"
     CDS             complement(2038..2370)
                     /locus_tag="IW819_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1245188.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IW819_002094"
     CDS             2518..3393
                     /locus_tag="IW819_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1245189.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IW819_002095"
     CDS             complement(3475..4056)
                     /locus_tag="IW819_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1245190.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IW819_002096"
     CDS             complement(4062..5321)
                     /locus_tag="IW819_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1245191.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IW819_002097"
     CDS             complement(5323..6369)
                     /locus_tag="IW819_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245192.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1805 a         1337 c         1101 g         2224 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctacc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaa
//
LOCUS       DACEPW010000134         6363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEPW010000134 DACEPW010000000
VERSION     DACEPW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.136"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(88..477)
                     /locus_tag="IW819_002098"
     CDS             complement(88..477)
                     /locus_tag="IW819_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1245193.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(537..1040)
                     /locus_tag="IW819_002099"
     CDS             complement(537..1040)
                     /locus_tag="IW819_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1245194.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(1053..1277)
                     /locus_tag="IW819_002100"
     CDS             complement(1053..1277)
                     /locus_tag="IW819_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245195.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(1381..3291)
                     /gene="ltrA"
                     /locus_tag="IW819_002101"
     CDS             complement(1381..3291)
                     /gene="ltrA"
                     /locus_tag="IW819_002101"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1245196.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(4037..4156)
                     /locus_tag="IW819_002102"
     CDS             complement(4037..4156)
                     /locus_tag="IW819_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1245197.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(4171..5355)
                     /locus_tag="IW819_002103"
     CDS             complement(4171..5355)
                     /locus_tag="IW819_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1245198.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(5614..5904)
                     /locus_tag="IW819_002104"
     CDS             complement(5614..5904)
                     /locus_tag="IW819_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245199.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(5894..>6363)
                     /locus_tag="IW819_002105"
     CDS             complement(5894..>6363)
                     /locus_tag="IW819_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1245200.1"
                     /translation="PARDGISVAGLLPGKFEQGNRVYEVTGTAPTLSTKQGGTKIMIR
                     KNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAIVTDQYQIRKLTPR
                     ECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVPVIFDIARRLKELDVHGL"
BASE COUNT         1744 a         1429 c         1007 g         2183 t
ORIGIN      
        1 gacaaaaagg agtaattgta aggtattaac tcatagtagg caaaacattc tccctcttga
       61 ttaaagacaa gattattttc aatatactta atcgggtaca tagttcacac tccttaccag
      121 agtaatgggt tcgtcccatc gtttcttctg ttctttgact ttctttcctg cgaaagtgac
      181 cttaaatcgt aagtgatagc taataaaaga tcgtaaaaat cccaatggtt tcttaccgtc
      241 aaacgttttt tgcgacatga accaagtaac tgccaccggt atacctaagt attttaaaaa
      301 ggccccctct atcatagata gtggaggcac atgggcaaat acgatgacaa acagcaagga
      361 taaaacaaac caagccatct ggttaaaagt cacaggaaaa ggcagttgaa agtcattaat
      421 tgcataaatg accttttcaa ctgcccaaat actggtgtag ctttttattt tcttcatcaa
      481 atcattcctt taaaaagaag gatagtttag aaatcaacta cccttcaata attttgttaa
      541 tatggatact caaaaacacc tcgactcgtt accaaataat tgcctccaat ctccaagtct
      601 ctaccacacg catcaaaatc aatatacgtc ttcaagtgtt ctggtacttc tccaaaatca
      661 ccagcttcta caatcaagaa atatgctaca tcactcatat catcgcaatc tggataatag
      721 ataatatcat ccacatgctc taccatctcc tcaaaagaat tgaagaaagc atgttgaatc
      781 tcagaagcaa cttcaccaac tggcgttcct tcaagttcct ccgctaagcc acacaaccga
      841 ttaatttcct caattggcgt atactcatca atctcaaagg gaagttcata atcatgaatt
      901 gcatattcct cgtattcgtc atttaaccca attcgttctt tcacttcatc aaaatcgaca
      961 ggtggggtaa accacgcccc caccaattca ccttcgttat atttcccgag gttcgcaata
     1021 taaacacgca tctgttccat cgtgcttccc ccttacgcac cgacaattcg gttaaacaaa
     1081 ctgagtaaga catccttcac cccagaagca ttaaagacca atccgaccgc aatcaaagca
     1141 atcaccaaaa agccaatcag tttggaaaac tcccgcttaa atcctaaaaa caaaccaatg
     1201 accacaattg ccagtaagac taaggattgg gcattggtta aaaaccattg atatatggat
     1261 aggtaagttc ctgatattat aaatatctca aacttttccc cccattcaca cggagcgtgc
     1321 gactttcacc gcactccgcg ttccatcgtg tttatattga aaagtattta tgtagtagac
     1381 ctatatcaag tttaattctt gattgccagc taatactcta tatttattta gtcttatgag
     1441 ctggacttta tttagcttca atagagctat atatttttgt attgtttctt ctgttttagc
     1501 gtgtattaga cggtgtaccg cctcttttac caaaataaga tttttataat cgtcatttcc
     1561 accagctcca gttggtacaa tatgatgaca atgtatttca ttagcttgtt gaaattcttc
     1621 cagagttata gcacattttc catgttgagc agaaaatagc gaaattcgat tgtctgcgta
     1681 ttctattgtg cgattttcca aagataattt cattaactgg tacatcagta atttatcaac
     1741 acttagattg gtgtggataa attttcttcc ctctagagtg tagcaattcg ctaaatacac
     1801 ctttgccatt ggttttctgt gttggatata tccaacaggg aaaataggtt cgccctcgcc
     1861 agcaacatac cgaagcattt tagatttacc atatcttgtt gcctcctttg tcgtaagttg
     1921 tctgcctgtt tttctcaatc tgcccctttt gtttgtacct aatctgttag tgaagatagc
     1981 cattacagct ctattcagtt tcatacaatc cagatttaca ttcgttgcaa tctgataata
     2041 attttgcata cctaatacca ttgaattata aagtcttgtt tcttctaatt ccgttttccc
     2101 ttttgaggga gaagcaattt tctttgcctg tgataccagc ttgtcttttt ggtttttgaa
     2161 ttgtttatca cttatatgtg atttaacaac taacttcttt cttctcggta tcattttaat
     2221 cttgaaacca agaaagtcgg agtatctttt tctaacattt acaattcttg ttttttcttg
     2281 tgatacttcc aattttaatc gttgttcaat ccattgagtg attgctattt tggctttttc
     2341 agcactttcc ttgtagcggc agaaaactct aaaatcatct gcataacgaa ccatatacat
     2401 ttcctttaac tttgttttct tcatttcttt gtaagcgttg cttttacatg ggctaccgct
     2461 tttattgata tgaaccacat agttttttgt aacaggattt tcctgccatt ggctttctac
     2521 ccaatggtct aactcattaa gaacaatgtt tgctagtagt ggggatatta ttcccccttg
     2581 cggagttcct ttctctggat atgttattgt accgttagtc attttgattg gagcttttaa
     2641 tatccttttt aaaatgaata tcaagtgctt gtcccgtatt cccatagccc atatctgttt
     2701 tatcagcttg gaatggttca cgttgtcaaa gaaaccctta atatcaaatt caatcacata
     2761 atgtagctgg ctgatttgta ggcggttata ggttgctttt attgcatttt caactgacct
     2821 attaggtcta aaaccatagc tgttttcact aaatttagct tcgcagattg gttctaatac
     2881 ttgttttata cattgttgta taagcctgtc ccacatacaa ggaataccta gaggtctggt
     2941 tttaccattt ggttttggta tttcttttcg cctcactggc ttaggtcgat acccatgtgg
     3001 actgcctgca acaatgtatc ttactttttt gacaactgtt tctgctggca atttgccaat
     3061 atctttaatt attgtaccgt ctgttcctgc tgttttactg cctgcatttg ctttgatatt
     3121 tcgatatgcc agtaatatat tttctctcga taggataata tccataagat gagtgaaaat
     3181 atctccattc ttacttttgg catataaatc atcaaaggtg ttttgcattt cgtaatattc
     3241 agcgtgtctt aaatcttcta cacacaatgt ttcattttta tttttaggca taaggcttca
     3301 ctcctttact tgaaattgag tgagcttttt tatcttactc gtaatcctta ttgaaacaac
     3361 ataatacttt tctaatatat acacgacttg agggcattcc tccattctca ttacaagaat
     3421 ttcttaggtt cgccctctgc ttttcagcat tagttcaact gcttattgtt cttcgtcagt
     3481 ttattctctt gatgagaacc caataccttt cctcgttccg ataatcttat ctatacatat
     3541 atatccttag gtcactagct ataagcctgc tggcttgata ccgcctgtaa cgttatatgg
     3601 catttcataa cctatattct tactttgcca caccagctac gatttctcgt acctttgcat
     3661 ttctacaaag ttcaaattta gacccgtaca ttcgctgttt cgtcagttcc ttatagaaca
     3721 ttctaaccat agatatttta tagacccccg acctgttgga tacgctaccc acctctggat
     3781 agttttcgat agaagatata tgttcatcaa ctattacggt atctttcagc cgtctttacc
     3841 gagcttcaaa cattctgagt ttcctcatac aatgcttgtc ggaggctcag ggcaggcgtt
     3901 tcagagcgtt acttcatcat tccacctgtc agattatcag ttctcctaat aaccaaactt
     3961 tttcgttctt ttattagtgc cttagctttt cactaaggaa cgagtcgcac ataaattttg
     4021 tccgaaattc atcttgtcac tttcctttct tttccattgc tcgatccgct gctgcacttg
     4081 cattcatcaa gcacatcaca gtaacaccaa gcatactgcc accaaatacc aaaaaaatat
     4141 cttttacaat tcccatgacg attcctcttt tcatataatc aaattttcaa ttgccatact
     4201 ttgttgctcc aaaatctttt tatgtcggtc tgataagcgt gcattctgta ccatatcctg
     4261 gatataggtt gtccgattca atcgatccaa ctccttagcc attttccacg taggggcgac
     4321 ttgtcggcct agccaattca aggttcgttc aatggtatac ggttttggct cggaagttaa
     4381 ttgaatctct ttccgattcc gtccaataaa acgcccccat tgttcattca agggccagtt
     4441 ggttcgcctt ttaccttcta ctttatccgc aaatcgcaag taacgattga taatggaaaa
     4501 cgtggtgctc tcaatgctac gaccttttaa caaatcttga atcgcatgaa aagcacggtc
     4561 atttttcagt cgaatctcaa aacgattttt cgtctcagtt tggtctaatg gaatccctaa
     4621 cttgatatat tgttcatagt ctttttcata caaacagaaa taaacctcac tcttgagact
     4681 gccgatatat aaggtattgc ccatgccgtc cttttcatca gcctttaaaa gttccccgga
     4741 acgataactt ttaaaggtac gaaacaagga gatacattct tccttctgac attttttcgt
     4801 tagctcaggt atatccaata gccccactcg atcattgatt gccaagtcca agcgtttcat
     4861 cacgccaccg gcctttagac aatcctcgaa aaagtcgtac cagctacgct tttgagcgag
     4921 taaaaaagtt tcaaattgtc ggcaacctcg cccctttaat tctaaaagca cacctttatc
     4981 ttcttcatga gacaacatca ccacaatatc acccatgaca tactgttctt gataggagta
     5041 aaaggcataa tcttcatgga gcatatactc aaatttcaaa tggagaattt cttgaataat
     5101 ttttcgtgca tccgttgtgg gaaagcgaat ccttacatag tcaaacagta acgtcaaagg
     5161 caaatcggga ttaaagcttt ccagttggtc aaaaattttc ctcatggttt ttatagatgc
     5221 ggttacgcca ccattttcta tctgatttaa gtacggccga ctaagtccac aagccgtagc
     5281 caatttattt tgtgaaacgc cgtattcgtt gcgtcggtct tttaatagcc gataatctaa
     5341 attccgttgt gccaagttct cacctccaaa ttcaaggtgt taactttttc aaatgagtta
     5401 acacctatac aaattactta ctatctttga tacaacaagc attcttaatg gttgcttact
     5461 tattttttgt gtttttgtac ccccctgtta gatacggggg tgagacatca tggtcggcta
     5521 acgccgtcca tgaacaggca cagcaccacg gaggatttct gcttcgcgaa tcctccgcgg
     5581 tcagcgccta acagtcagtt gtgttggttt aggttactca tctgtaatac catttacttc
     5641 caactctgct ttcatttcta acaagtattg acgaatatct ggttcttttg cttggtccgc
     5701 aatccgctca aattctgcaa ttacttgatt tatttcaatt cgagcatccg tctcctcatt
     5761 cattaaatat ggatcgccag cgactttgac accattcttg cgaatatcaa attttaaccg
     5821 tcgcaattgt tcttctaatt ctgggtctga ctgaatcttt ccaatcaccc aataaatttc
     5881 tccaatcaac tcatcataaa ccatgtacat ccaactcttt taatcttctg gcaatatcaa
     5941 aaatgaccgg aactgttaca gagtttccgg cttgcttata caactgacta tctgaattga
     6001 cttgagaatc tcgatcaaaa gcccaatcgg gaaagccctg aagtcgccaa cactctctag
     6061 gtgttagctt ccgaatttga tactgatccg tgacgatcgc ttgttggtct cctgttagta
     6121 aggtatgaac actctgtttt cccagacgac ctcggcgact tgtggaatta ggaaaagcca
     6181 gattcacact gtcttctggc ttcacctctt gatattctgt ttcatttttt cgaatcatga
     6241 tttttgtacc accttgtttc gttgacaagg tgggggcggt tccagtaact tcataaactc
     6301 gattaccttg ttcaaacttt cccggtaata gtcctgctac agagattcca tcccttgctg
     6361 gca
//
LOCUS       DACEPW010000135         6322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEPW010000135 DACEPW010000000
VERSION     DACEPW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.137"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1165
                     /locus_tag="IW819_002106"
     CDS             <1..1165
                     /locus_tag="IW819_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. The beta-hairpin of the
                     Uvr-B subunit is inserted between the strands, where it
                     probes for the presence of a lesion; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAQ1245201.1"
                     /translation="VLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGR
                     KEGEPPYTLLDFFPEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDN
                     RPLRLEEFEKHVNQIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQID
                     DLVGEINERVEKDQRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRD
                     LRLGEFDVLVGINLLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGK
                     VIMYADKVTDSMRLAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDT
                     KEAVQVSYEEMTKEEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            1175..3991
                     /gene="uvrA"
                     /locus_tag="IW819_002107"
     CDS             1175..3991
                     /gene="uvrA"
                     /locus_tag="IW819_002107"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1245202.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            4254..5519
                     /locus_tag="IW819_002108"
     CDS             4254..5519
                     /locus_tag="IW819_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1245203.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            5523..6254
                     /locus_tag="IW819_002109"
     CDS             5523..6254
                     /locus_tag="IW819_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1245204.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
BASE COUNT         2124 a         1046 c         1414 g         1738 t
ORIGIN      
        1 tgtcttgcga aatgaaaaca aacttctgga agctcaacgt ttagaacagc gaaccaatta
       61 cgatattgaa atgatgttag aaatgggcta cacgtcggga attgaaaatt attctcggca
      121 tatggacgga cgtaaagaag gtgaaccgcc ttatacgcta ttggatttct ttcctgaaga
      181 ttttttgatc gtggcggacg aatcgcacgt aaccatgcct caaatcagag gaatgtataa
      241 tggtgaccga gcgagaaaac agatgctagt agattacggt ttccgtttgc catctgcttt
      301 agacaatcgg ccacttcggt tagaagagtt tgaaaaacat gtcaatcaga tcatttatgt
      361 ctctgcgacg ccaggaccat atgaacatga acaaacagat accgttatcc aacaaatcat
      421 ccgtccgaca gggctgcttg acccagtgat cgaagttcgt ccaatcatgg gacaaatcga
      481 tgatctagta ggcgaaataa atgaacgagt cgaaaaagac cagcgggtgt ttgtaacgac
      541 attgacgaaa aaaatggcag aagacctcac agattatttc aaagaattag ggatcaaagt
      601 caaatatttg catagtgata tcaaaacgct ggaacgaaca gaaatcattc gagatcttcg
      661 attaggtgaa tttgatgtgt tagttggaat caatttgctt cgtgaaggat tagacgtacc
      721 tgaagtttcc cttgttgcta tcttggatgc ggataaagaa ggctttttac gtagtgaacg
      781 ttccttagtc caaacgattg gacgagctgc tcggaatgaa gaaggtaaag taatcatgta
      841 cgcagacaaa gtaacagatt ctatgcgtct agcgatggat gaaacgtcaa gacgtcgtac
      901 gattcagcag aagtacaatg aagaacatgg gatcgttcca aaaacgatca ttaaagaaat
      961 ccgagatttg atttctatta cgaaagaatc ggaagatgat acaaaagaag cggtacaagt
     1021 ctcgtacgaa gaaatgacga aggaagaaaa agacacgtta ttgatgaaat tagaaaaaga
     1081 aatgaaagat gcggcaaaag ctttggattt tgaaacagcc gcgaatgtgc gagatatgat
     1141 tttagaatta aaagcttcaa agtaggagga caacatggct aacgataaaa ttacgattca
     1201 tggtgcacgt gcccataact tgaaaaatat agatgtaacg attccacgag ataaattcgt
     1261 ggtcgttaca ggtttgtccg gttctggaaa aagttcccta gcatttgata cgctgtacgc
     1321 tgaagggcag cggagatacg tagagagcct ttctgcttat gctcgtcagt ttttaggaca
     1381 aatggataag ccggacgtag atagcatcga cggattgagt ccggcgattt ctattgatca
     1441 gaaaacaaca agtaaaaatc ctcggtcgac agtaggtact gtgacggaaa tcaatgacta
     1501 tttacgcttg ttgtttgcac gtgtaggaca tccgatttgt cccaacgatc atatcgagat
     1561 taccagtcaa tcagtagatc aaatggcaga tcaagtaatg gctttgcctg agcgcacaaa
     1621 gatccaagtg ttggcaccga tcgtggtaaa gaaaaaagga caacataaaa aaatattcga
     1681 acgaatccaa aaagagggat acgtccgcgt tcgagtcgat ggtgagatct atgatttaag
     1741 tgaagtgcct gagctggaga aaaacaaaaa acatgatatc gcgatcgtca tcgaccggat
     1801 cattgtgaaa gaagggattc gttctcgcct atttgattct ttagaggcgg ccctgcgatt
     1861 agcagacggc tatgctctgg ttgacgtgat cggtgaagaa gaaatgttgt ttagcgaaca
     1921 ctatgcctgt ccttattgcg gatttactgt tggcgaacta gaaccaagac tgttctcatt
     1981 caatgcgcct tttggagctt gtccagactg tgatggttta ggtgtcaaat tagaagtcga
     2041 tcaagattta gtgattccag atagaacgaa gacacttagt caaggtgcta tcgttccatg
     2101 gaatccaatc agctctcagt attatcctca aatgttagcg caggcttgtg aaagtttcgg
     2161 tatcgattta gatacaccat tcgaagaatt accggaagag catcaggaga tcatcttgaa
     2221 tgggacagat gatcttttcc atttccatta tgaaaatgat tttggtggcg ttcgcgatgt
     2281 agaaacgact ttcgaagggg tattgaagaa tatcgagcgt cgttatcatg atacgaacag
     2341 tgatttcacg cgtgagcaaa tgcgtcttta tatgacagaa ttaacttgtc agacatgtaa
     2401 aggttatcga ttgaatcccc aagctctatc tgttcaaatc aatggaacga atatcgggca
     2461 aacaaatgag ttgtctattc aaaaagcagt agatttcttc tcaaacttaa cgttatctga
     2521 acaagagaaa gtaattgcca aaccgatttt aaaagaagta aatgacagac tttcattctt
     2581 ggaaaatgtg ggactagact atctaacttt gagtcgcgct tccggaacat tatctggggg
     2641 agaagcgcag cgaatcagat tggctacaca aattggatcg aatctttctg gagttttgta
     2701 tattttagat gaaccatcga tcggattgca tcaacgcgac aacgatcgac tgattggctc
     2761 attaaagaaa atgcgtgatc ttggcaacac cctcgttgta gtcgaacatg atgaagatac
     2821 gatgtgggca gcagattatt tgattgacgt cggacctggg gccggagaac aaggcggaca
     2881 aatcgtggct gcaggcactc ctgaagaagt agctaatgat gaaaattctt tgacagggaa
     2941 atatctttca gggaaaaaat caatccctgt accaaaagaa cgccgtaaag gtaatgggaa
     3001 agcaatcaag atcactggtg cttcagaaaa caatctgaaa aatattagtg ttgaatttcc
     3061 attaggagaa tttgtagcag tcacaggtgt gtctggttct ggaaagagta cattggtcaa
     3121 tagtatctta aagaaatcgt tagcgcaaaa attaaataag aattctgcta agccaggtaa
     3181 attcaagaca atttccggct acgaaagtat cgaaaagatc atcgatatcg atcaaagccc
     3241 aatcggccgg acgccgagaa gtaatccagc gacttataca agtgtatttg atgatatccg
     3301 tgggttattt gctcaaacga acgaggcaaa aatgcggggt tataagaaag ggcgttttag
     3361 tttcaacgta aaaggcggtc gttgtgaagc ttgtcgcggg gatggaatta ttaagatcga
     3421 aatgcacttt ttgcctgatg tctatgttcc ttgtgaagta tgtcatggta aacgatataa
     3481 ttctgaaaca ttagaagtgc attacaaagg aaaaagcatt gctgatattt tggaaatgac
     3541 agtagaggat gctgtagaat tcttcaagca cattccaaag attcatcgca aactgcaaac
     3601 gattgttgat gttggcttag gttatgtgac gatggggcaa ccagcaacga cattgtccgg
     3661 tggtgaggca caacggatga aacttgccag tgaattgcac aaaatctcta atggaaagaa
     3721 tttctatata ctagatgaac caacgacagg acttcatagc gatgacatcg cccgcttgtt
     3781 gcatgtatta caaagattag tagatgctgg taacacagtt ttagtgattg aacacaatct
     3841 agatgtaatc aaaacagcag attatatcat tgatttagga ccagaaggtg gagaaggtgg
     3901 aggaacgatc cttgcgactg gaacaccaga agaaatcatt aacgtaaaag aaagttatac
     3961 aggtcactat ttgaaaaaaa taatggtata aagaggatag atagctgaga tagttaaaaa
     4021 aacataaaaa aatgaatttt tgcttgtttt attaatatca gtgttctaga atcattacat
     4081 ggtagttctt tctacactga ttggtataga taaaaacttc ctataagttc aatccgttca
     4141 aaaaagaatt gagcaatcgg aggactttcc tttgattgct tttttcttta tggtatgata
     4201 ttccttgtgt agttggaaaa gaaaaacaag agattatgag taagtgagag ataatgatga
     4261 acagtattga aaatgaagaa tttatcccta tattattagg aagcgacatg aacgtttatg
     4321 gtatggcacg ttcttttaat gaagcatacg gcaaaatctg ccaagcatat gcttcggatc
     4381 agttagcccc tacccgctac agtaaaattg tcaatgtaga agttattcca ggatttgata
     4441 aagaccctgt atttatcgag acgatgcttc gtttggcaaa agaaagatat tctgataaga
     4501 gtaaaaaata tcttttgatt gcttgtggtg atggatatgc agaattgatc tcccaacata
     4561 aacaagagct gtcagaatat tttatctgtc catatattga ttattcttta tttgaaaggc
     4621 tgatcaataa agtaagcttt tatgaagttt gtgaagaata tgatcttccg tatcctaaaa
     4681 cattgatcgt cagagaagaa atgttagtaa atggacattt ggaacaggaa ttgccttttg
     4741 aattcccagt tgctttgaaa cctgcaaaca gtgtggagta tctttctgtc caatttgaag
     4801 gaagaaaaaa agcttttatt ttagagacta gagaagagtt cgatttaatt ttaggccgga
     4861 tatacgaagc cggttataaa agtgaaatga ttgtacaaga ttttatcccg ggtgatgata
     4921 gtaatatgcg tgtgctaaat gcgtatgtag atgaagatca tcaagttcgg atgatgtgcc
     4981 ttggtcatcc actattagaa gatcctacac cagcatcgat tggtaattac gttgtgatca
     5041 tgccagatta taacgaaaaa atctatcaaa ccattaaggc atttttggaa aaaatcgaat
     5101 atacaggctt tgccaatttc gatatgaagt atgatcctag agatggagaa tataaattat
     5161 ttgaaatcaa tttgagacaa ggtcgtagca gtttctttgt gacattgaac ggcttgaatc
     5221 tggctcgttt tgtcacagaa gatcgagtgt tcaacaaacc ctttgttgaa acgacctatg
     5281 gaacgaatca atcagataaa gcaagattat ggatgggtgt gcccaaaaaa atctttttag
     5341 aatatgctcg ggaaaatgaa gacaaaaaac tagctgagca aatgatcaaa gaaaatcgtt
     5401 acggtactac tgtattttat gaaaaagacc gatcaatcaa gcgctggttg ttgatgaaat
     5461 atatgttcca taactatatt ccaagattca aaaagtattt tcatgtgaaa gaaggctagt
     5521 tcatggagaa ttttttcagt attttaggcg gaatgggcac gatggcgaca gaaagttttg
     5581 ttcgcttgat caaccaccga acgaaagcta caaaagatca agaatatttg aattatgtct
     5641 tattcaatca tgcaacagtt cctgatcgta cagcttatat tttagatcga tcagaagaaa
     5701 atccaatgcc gtttctgctg gatgatattg agaaacaaaa tttattgcga ccaaacttca
     5761 ttgtgttgac ttgtaacacc gcacactact tttttgaaga gttacaagcg gcaacggata
     5821 ttcctatctt gcatatgcca agagaagcag caaatgaact tgtccgtcag catacgacag
     5881 ggagagtagc gattctagga acagaaggaa gtatgaaagc tggaatctat gaacgcgaag
     5941 tcaaaaatct tggtttcgaa acagtgattc ctgatactgc gcttcaagaa aaaatcaatt
     6001 acctgatcta tcatgagatc aaggaatcgg attatttgaa tcaagagctc tattacgaaa
     6061 tcttagagga agcggtagag cgcctaaact gtgaaaaagt catcctaggt tgcacagaat
     6121 tatctttgat gcatgaattt gctgaagata atcattaccc agtgatcgat gctcaatcta
     6181 ttttagcgga tcgaacgatc gaacgtgcat tagctgaaag aagcgaggca ttggatacag
     6241 catcggaaaa gtaaaaaaat tggatttggg gcaaatatcc taaaagatat ttggtccaaa
     6301 tctttttgta aaagaaaaag aa
//
LOCUS       DACEPW010000136         6258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEPW010000136 DACEPW010000000
VERSION     DACEPW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.138"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(504..1313)
                     /locus_tag="IW819_002110"
     CDS             complement(504..1313)
                     /locus_tag="IW819_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1245205.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1313..2059)
                     /locus_tag="IW819_002111"
     CDS             complement(1313..2059)
                     /locus_tag="IW819_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1245206.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2077..2544)
                     /locus_tag="IW819_002112"
     CDS             complement(2077..2544)
                     /locus_tag="IW819_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1245207.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2544..2954)
                     /locus_tag="IW819_002113"
     CDS             complement(2544..2954)
                     /locus_tag="IW819_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1245208.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2965..3978)
                     /locus_tag="IW819_002114"
     CDS             complement(2965..3978)
                     /locus_tag="IW819_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1245209.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4195..4929
                     /locus_tag="IW819_002115"
     CDS             4195..4929
                     /locus_tag="IW819_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1245210.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVRNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4913..5611
                     /locus_tag="IW819_002116"
     CDS             4913..5611
                     /locus_tag="IW819_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1245211.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSHS"
BASE COUNT         1991 a         1313 c         1070 g         1884 t
ORIGIN      
        1 gcaaaacatc agcttaaagc gacaaaaacc acgagcaggt ccattcatcc tcaaacctcg
       61 gtgatcttcg cggtttaaca ccatcagaag aaatgcacaa aaataataag gagagtctga
      121 gacataaata aattcccaga cttttagtga gtctaaatcc gaataaacgg tgggtcgaaa
      181 gcaactctag ctgcgcgtcg atcgcagcaa tctctaggtg ctatgttcgg ataaatggtg
      241 gtcaaaagtc agattttcgg ccatttcgta acagccaacc agacaaaaag cgtctgtttt
      301 gtcttctact ccaattgctc aaatcttagc gacttttggc ttggtcttac tctaaacagt
      361 ttgtaagcct aaactttatt tgctgtttgt ttctaagatc aaagcaaaac tactttttag
      421 ttggttactt ttacaagttc tttttactgt tgaataaatg taaatcttaa gaacaaatat
      481 ttacttctat aattccaagt ttcttaaaaa attccgccaa gtgtacctaa aaagcataaa
      541 acaaaaattc ctagaataat tgtgatcgtt ttatgccctt tggagtaaag ccagacgcat
      601 aaaaaggtaa ttcctaatgg cagcaagccc ggaaaaattt gatcaaaaat actttgtaat
      661 gaagtttcag ctccaccaac cgtgaatttt agtgacgtat ttaaagtaac catcgtgcct
      721 atcatcgcgc caattaccgt taaacctaaa atcgaagcac catgagataa tttgtcaatc
      781 atatggttgt cttctgtatc atcaataaat ttagttccta aaacatagcc atattttgtc
      841 gccaaccatt taaaaataaa atgaatggca gtatacaaag taaaataaag gatcatgcct
      901 aaaatattcc cttgttgagc aaaagaaata cctatgccag ccgcaataac ccgaaaagtt
      961 ccccagaaaa atgaatcgcc aatacctgat aaaggtccca ttaatcctgt tttgatttta
     1021 ttaattgcgt ctgtatcaaa ttcaggattt ttagcatttt cttcttccat ggtgacggac
     1081 aagctcataa caaaaggcgc aatatgcggt gtaatgttaa atgtttccgt atggcgctgc
     1141 aacgctgtat aataatcatc atcgttcgga tatattttac gtaaggaacg ttcaatacca
     1201 ttcatttgtc catatgccat cattttatcg aaggaccaag aggattgcat aaaaaaggag
     1261 cgccaaaata tacttcttaa ttcttttttc ccaataacac tttcttttac cattataaat
     1321 cttcctcctc ttcaaaagtt agcttttgaa cagccagtaa cgctaagcaa agtccgacga
     1381 cagccacacc gacaacatcc attttcagat acattacgcc gacaaatcct aagaaaagat
     1441 aaggcatcat cgctttattc gccatcattt taattaataa cgctagccca acacaagaaa
     1501 gcacaccggc cgcaacatca aagctgtcca atacaacctg aggaatcaac ttgatcatcg
     1561 cattgattgt agacacacca aaataagtag ctacaaaaac taaaatggcc gatggaaccc
     1621 caacgattaa aggttgagcc acataatgat atttttgtgc ttttttgaaa tcatgattga
     1681 ctaatgcctc atccattttg ttgttaaaat acgtaattgg cattgcatag gcgaagttac
     1741 gccacatctg aagaaagatg gacaaaggta aagtcaacgc aacggcagtt cctactcctt
     1801 gacccgattg aatcgcaatt gctgtaccta aaacactgga agcataaact tcagggggcg
     1861 tcgcactccc taccataatt gagcccatga acatggattc caaggtggca ccaataataa
     1921 ttcctgactc taaatctccc aaaatcaaac cgacaaaagg cgctgtaaat agcggtctac
     1981 ccatcatcga tgcaccaaat gtgtattcat caatagacgc caaaaaagct accagtgcga
     2041 ttaatattgc ttgtatcata ttcttcccct cctcttttag tcatgcaaag gattgatttt
     2101 tttgtccgtt ggtacagcct gacattgaac tgttatcccg ttttcagtca tttttttcac
     2161 caagtccagt tcttgttcat tcaagtaaac ggaactagta atcattttcc gattctcgtt
     2221 tttctttaaa tttcctaaat tcacttcttt aatctcaggt acgtgctgag taatttcgta
     2281 ggcatcctcc accgtcccga caattaaaaa tacttggtat ttcttgattt tttctccatt
     2341 taaaacagag atggcctctg tcatatcctt caccacaaat tttgtccctg taggaacagc
     2401 catttttagt gtttttttgc gcagattatt ggtagcgatt tcattacttg caacgaaaat
     2461 acaatttgca cctaagttat ttgtccaggc aaaagcaact tgcccatgaa caaaacgttc
     2521 atctactcgt aataatttaa tcattaaaaa tcatcctctt ctgttttctc tagcaactcg
     2581 ttcaccaaaa ctatcccttc attcccgcta ttcatagctt tttggattag ttttttcgtg
     2641 tcctgttcac ctgataagca aagctctaaa agcagcggca gatttacacc gctgattaaa
     2701 taaaaattgt ctttaccgat ttgttcggag aaagcgttag acacgcttcc tcccagtaaa
     2761 tctaccgtca caattaattc gtcatcaggc gctaagtcgg aaaatagctg ctctatttcc
     2821 tggttaatgg agtagtcact atctatatac gcagttattg ctaaaagtga tttttgagga
     2881 ccaataataa attctaaagt atcctttaag cctttagcca tcatggaatg cgtcgcaatt
     2941 acaattttcc tcatagctct taccttaata ttccacagtc cgataataac gacgaatatc
     3001 tagtgaatga tcccgcacct tttcaaaatg agcactcact cgttctaagg ccgttgccat
     3061 aaccattggc gctacatact gacgcatagc gcttggtaca cctgctaatt cgaaatccgc
     3121 agtgtcaaaa accgtcacaa catcgctata tttatttaca aaacgctcta cgcgatcgac
     3181 tagcggacgc gtttgatctt caccttttaa taacacaaaa ctcatagatt tatcagtcat
     3241 ttcaatagtc ccatggaaaa attcggctgc atggatcgac ttggtggcaa tccattgcat
     3301 ttcttccagt acgcacatag catattcata ggcagcccca taagtattcc cggctccaac
     3361 aaacatatga aattccgtat ttttatgttt ttctgcaaat tcaagtgctt tctgatcatt
     3421 ttgttgccga acacttagta atacttccgg cattttttgt aatgtttgaa taaattcatc
     3481 atattcagga aactcaccgt tatttttcat caggcgtgca cccaaagcaa aaaactgtaa
     3541 ctgaagttct tctactaatg tatcgttgtc agcatcattt tcaatcgcat aatccacgat
     3601 ttccccgagt ggtgtagcag catttcctac aaacgcaatc gtcgttgcac cttgactatt
     3661 ggcatactta acaacatcaa cggtttcttg agtcgtaccg gttaatgaag atgtgattaa
     3721 cactgtatct ttattaatct ttttcggctg tgaacaaacc acctctgcgg ctatttcatg
     3781 ataataatca agctctgaat gagtgcttaa cagataagct agcgggctga ttgtggcata
     3841 tgttccacca gagcctacca aataaagatt tttaaatccc ttctcactga ttgcatctgc
     3901 cacctgattg attgtctcga tattttttac tgcaccattt aatactgata gaaaacgttc
     3961 ttcgctaaaa cctaacatag ttaaaaacct ccaagtcaat aatttgaaaa atatatttgc
     4021 aaatgccggc acattaacaa accacttaat cttttttata taccacctgt taacgttttt
     4081 aatgtaccac ttatagaaaa cgctgtcaat agtttttaac cacttttatt tttatttgta
     4141 ccaaaatcct tttttcatgt ataataaaca aaaatagact ctggagtgta acgtatgaat
     4201 actgaagaac taacgaacaa attaatacaa gacattgaac aagaaaaata taaatacgcg
     4261 caagctattc cttcagaacg tgattttgtt caaatttatg aaatgaatcg ttcagccgta
     4321 aaaaaagcca ttcaacaatt agtaaataaa gagtacctac taaaaataca tggcaaagga
     4381 acttttgtta aaaaaaatat ccatgaacat ttgcaaatca aatttcatgg tttgtctgaa
     4441 ttgttggaac gggaagggat tcagcctagc agcttaattt tagcttcaga aataaaaccg
     4501 gctggacaca ttgtcgggaa agctttacag ctggaaccaa ctgaagaagt atttcatttg
     4561 gttcgtttaa gaagcggcga cggtgagcca atttctattg aagacactta tattccatta
     4621 aatctcatac ctgatcttca agcttttgat ttccaaattt tttcatttta ttccacattg
     4681 gctgcttatc attatcaaat tgaaaatatc tatcagacaa tttccagctc aaaagtgcgc
     4741 aataaagaag cccgtttgtt aaaaaccagc ttaaatcgtc cggtaatcag tcttgctatc
     4801 actgcggtta cgacaaatga acaagcggtt gaatacactg aagtagtggt tttgcctgaa
     4861 ttcgccgcca tctatactaa aaatgttcat caaggaaagg agatgaaaat ctatgcccaa
     4921 attgattaat ctgttggctt atgacctgat tgacaaaatt attgcttata ttcatgaaca
     4981 tcatttaacc cccggaacaa aactgccttc ggaacgggac ctatcagaaa tgtttaatgt
     5041 taatcgaact accttaagag gcgcgttaca aaaactggtg gacaacggca ccttacgggc
     5101 gcaaaccaat agcggctatt atttaactgc gccaaaattc gaaaaaaatg cccatcattt
     5161 ctttacaccg atagaagatt ctctgcttag taagcagcgg ctacagctaa aggcaacggc
     5221 tctttcaccg aaaaaagcag cagctctttg tccggaactg aatgatttca aacagcagca
     5281 accacaagct tatttagaat atattgatgc agaagcaatt agcttgatgt tattaatccc
     5341 tatggaaaca ttaaagaaaa gtaatttctc ggacaatcct ttccaagatt ttaaaagttt
     5401 ggcatatacg cgttctcaag aaatttcttt aataactgac aatgacgttg cggcggactt
     5461 gctgggagtt gacaaagaag aggttctgtt atttgtacga gaggaaataa gagccggtaa
     5521 ggagttggcg gcgattgctt taggttatac tattagtcgc cgctgcaaca tacaggttaa
     5581 tgttgctacg accttgcaat cccatagtta ggaaaaacta ttcgctgtaa gttcaaccgt
     5641 ttcaatgcaa gcgaatcaca aatcaaaaag tatttaactc gtgtttatta gaaatagtca
     5701 tgaactttta gtctccaaga tcaaaccgac agcgttcaac tctgagaagc aaaagtaaat
     5761 gtacgatcaa gtatgaggct gggacagaag cgctaagctc cgagaaccaa gagaaggtac
     5821 gttcattatc gaatcactgc attcttcgtg tttatcagca atttcggctt atttccgcag
     5881 gccttcaaga aggttgaagc aaaagtcgtt cagatttgag caattggagg aaaaggcgct
     5941 ccagacgttc cttgtctgga gcggctgtta tgaaatggcc gaagatctga cttttgacca
     6001 ccgtttaatc aaggttgagc caaaagtcgt tcaggtttga gcaattggag tagaagacaa
     6061 aacagatgtt ttttgtctgg ttggctgtta cgaaattgcc gaagaggctg atttttaacc
     6121 accgtttatc tagtcatagc acttagagat tgatgcgatc gacgcgtagc tagagtggct
     6181 gccgctgcaa tgacccgtgg caaaatatac tgctttttca tttttcggtt ctgttgcaaa
     6241 gttttaaatc tactatca
//
LOCUS       DACEPW010000137         6058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEPW010000137 DACEPW010000000
VERSION     DACEPW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.139"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            822..1556
                     /locus_tag="IW819_002117"
     CDS             822..1556
                     /locus_tag="IW819_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1245212.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1572..2741
                     /locus_tag="IW819_002118"
     CDS             1572..2741
                     /locus_tag="IW819_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1245213.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2757..3752
                     /gene="lacD"
                     /locus_tag="IW819_002119"
     CDS             2757..3752
                     /gene="lacD"
                     /locus_tag="IW819_002119"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1245214.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3831..4757
                     /locus_tag="IW819_002120"
     CDS             3831..4757
                     /locus_tag="IW819_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1245215.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4776..6026
                     /locus_tag="IW819_002121"
     CDS             4776..6026
                     /locus_tag="IW819_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1245216.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2024 a          995 c         1243 g         1796 t
ORIGIN      
        1 cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta actttaactg
       61 ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac aatcggaata
      121 gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt gccattccaa
      181 agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt gactaaacga
      241 agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat tcagataaac
      301 tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc ttaagagaga
      361 acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat ttattctcat
      421 agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga tttgaaacca
      481 aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca ctggtaagta
      541 gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta aaaaaagata
      601 ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa aagttggaaa
      661 attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat attaacaaga
      721 ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa ataccactac
      781 tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat acctttcaat
      841 caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa aacagtatgg
      901 tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc gttagtcgca
      961 cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat cgacaacatg
     1021 gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct ggcgcatata
     1081 gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata ttggccttca
     1141 agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta ggtgagcctt
     1201 tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc gagaaatcct
     1261 atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg aaacctttat
     1321 ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag gaattttatg
     1381 ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga tcaccagtgc
     1441 tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt acattaagtg
     1501 tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact aactgaattg
     1561 gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga gcagaaaata
     1621 ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac cgttatcaag
     1681 aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct ggagagcgac
     1741 caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat acactaattc
     1801 cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt ggttcgaccg
     1861 atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc ttggtctcat
     1921 ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc aatcagctgg
     1981 tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg gctacacaaa
     2041 tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat gacgctggtt
     2101 ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt tttgatcaag
     2161 caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga gaagaagtca
     2221 tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc atcatttact
     2281 taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt ctcgagttaa
     2341 ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat ggtcccaaat
     2401 cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca tatacgcaga
     2461 aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa cgcgttgttg
     2521 gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt gcagattcgt
     2581 tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg atcgcattac
     2641 tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga acagtcaatc
     2701 gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg tgaagcatgt
     2761 tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca gaaggcatta
     2821 ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg gctttaggga
     2881 tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa gaattgactc
     2941 cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca cagaagcgtg
     3001 cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa accgtacccg
     3061 gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag ttaggtgcgg
     3121 atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc aatcggcaaa
     3181 agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg ccattttatt
     3241 tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc tatgccaaag
     3301 caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag tataaagtag
     3361 atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat gcaacagggg
     3421 agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc gatgcaacag
     3481 atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg gagacattgc
     3541 gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga gcaacttgga
     3601 aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa tggctgacag
     3661 aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact gccatcccat
     3721 ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt agtgataaga
     3781 gatatgtgtg attataattt atcattattg aaactgggag gatattttat atgaataaaa
     3841 gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa ttaaagcagg
     3901 caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt agctgtcctt
     3961 acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct gatttaattt
     4021 tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta actctaaaag
     4081 agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta aatttagaac
     4141 ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt ggtagaatcg
     4201 tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt tgtttaactg
     4261 gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca ttatccaaat
     4321 caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt gatgaaccaa
     4381 ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt ttattagttc
     4441 ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc gttgattata
     4501 taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta acggctaatg
     4561 gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta gcatcaaaag
     4621 catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt cttccagaaa
     4681 atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt atgttaggaa
     4741 aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt caaaaaatag
     4801 ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg gctgcagtta
     4861 tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg atagaaggat
     4921 ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca tcagattcaa
     4981 catttggtat tattggatta gctgttacct actctttggg acacaatcta gcaaagactt
     5041 atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt ttacttacac
     5101 ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt acctcgggtc
     5161 tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg tttgtactta
     5221 aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga gctttcgttg
     5281 ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta atttgttata
     5341 aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa ccactatctt
     5401 tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt ttcttttgga
     5461 tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta ttattgcaaa
     5521 actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac attataactt
     5581 atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt gcacttgcca
     5641 ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag ttatcaatcg
     5701 ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact atattaaata
     5761 taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt acttattttt
     5821 caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg acaactccac
     5881 cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg attcaagtaa
     5941 taacaattgc aataaatgtt ttgatatact atccattctt taaattagat gataatgcca
     6001 agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt agttaagt
//
LOCUS       DACEPW010000138         6018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEPW010000138 DACEPW010000000
VERSION     DACEPW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.140"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..442
                     /locus_tag="IW819_002122"
     CDS             <1..442
                     /locus_tag="IW819_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1245217.1"
                     /translation="GKELVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQE
                     KIEKIDSGFMLPYRVRFYDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDK
                     EVEYGNEIESHFEQIPIENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(597..1565)
                     /locus_tag="IW819_002123"
     CDS             complement(597..1565)
                     /locus_tag="IW819_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1245218.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1797..2630
                     /locus_tag="IW819_002124"
     CDS             1797..2630
                     /locus_tag="IW819_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1245219.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2664..3503
                     /locus_tag="IW819_002125"
     CDS             2664..3503
                     /locus_tag="IW819_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1245220.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3529..4089
                     /locus_tag="IW819_002126"
     CDS             3529..4089
                     /locus_tag="IW819_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1245221.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            4288..6009
                     /locus_tag="IW819_002127"
     CDS             4288..6009
                     /locus_tag="IW819_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1245222.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
BASE COUNT         2084 a         1029 c         1233 g         1672 t
ORIGIN      
        1 tgggaaagaa ttagtcaaaa tagaatctgt tttcgtattg atggattttg tgaatcgaaa
       61 aatgagcagt gttaatgaag agatcattgc tccttttgag agtgagaaga tcaagaaaat
      121 caagcgtcag gaaaaaattg aaaagattga ttcgggcttc atgttgccat atcgtgttcg
      181 tttttatgat atcgatagta atcagcatgt taataatgcg atgtatttta attggatcat
      241 tgacgtatta ggatatgact ttttgactac gcatgttcca gaaaaagtaa ttattcgatt
      301 tgataaagaa gtagagtacg ggaatgagat cgaaagccat tttgaacaaa tccctatcga
      361 aaatggtgtc aaaacaaaac atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa
      421 tattatatgg aaaaataaat aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat
      481 aagaagaaat ttacgaaaat tgtttctcaa atttttgtaa attttcgact tagttttcga
      541 agaagctatt tttgtcacag cctctctttt taagtttagc tattagataa tgatgtttag
      601 ttttggataa acgaaatcaa ttcttcaccc gacaaaccat agtttacagc aatcaatagt
      661 tttaaacgtg ctttttgact attaatactg ttacagaaaa taatccccat tttttctagt
      721 tcttttcccc cgccttcata atcataaaca ggctctgcga ttccattgaa acaacgagaa
      781 acaagtacta cagggatttt ttggacaaga agcttttcca aagcagctaa ggcttgtgga
      841 gggagattcc ccgcacctaa agcttcgatt accaaaccgt ctactttcgt atgggcgatg
      901 gcttccaata agtccccttg catcccagca tatgctttaa cgatcgggat agtcccatct
      961 acagcggaaa tgtccagtcg ctttgtttct aaaagttcct gcaagaataa aattcgattc
     1021 ttagttacta agccgatggg acccaatgta ggtgttcgga aagtagcaac atttgtggta
     1081 tgggtctttg tcacgtagcg tgcagaatgg atctcatcgt tcatgaccac caaaaccccc
     1141 ttatctagcg cttcttcaca acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg
     1201 tcacttccaa gttcattact tgaacgcatt gccccagtca aaacaattgg taatttgttg
     1261 ccaatcgttg tatcaagaaa aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc
     1321 accactccgg atatacctga tgcataagcg gacaagatac gttcttttaa taccaacatg
     1381 tgagaaggca tgatgtgtgg tgagggaaga ttgaagatat cttcaactaa taattctaca
     1441 tgcttgggga ttgtgatttc tgcatctaat aatgggtttg ccacatcagg actaacaccg
     1501 cccgtgagca tgttttcttt catggcgatc gttccgccgg tgtgtagtac aagtattttt
     1561 ttcattttac agacttcctt acgaataatt ttgcatagcc gttaaatctt attgtatact
     1621 atataaaaga gttttgctat cagtgaagaa taacgaatag cctaacttat cataaaaaag
     1681 ctgaccttat taaaatggaa aagactcaac cttgacctcc taatacaatg gagtatctgg
     1741 ttgagtttct tttacaagaa agcagccaag gaaatcaatc acgaaggtgg ccggaaatga
     1801 tcagaatcgg attaacatcg tttaaagaac atgacaaatt aactggtaaa aagaataaca
     1861 ctctttatga atatgcaggg tttctaccct tagtggaatt agatactgcc tattatggaa
     1921 ttccccgaaa gtcctcagtt gagaattgga taagtgaagt acctgaatca tttcgctttg
     1981 tagtcaaagt ctatcaagga atcagcggtc aaggagactg gaagccttat tatcagaccg
     2041 aagaagagat gattgcgcat tttttagatg ttatgtcgcc aatggtagat gctggaaaga
     2101 tgttttgctt tttagtccaa ttcccctcat cattcaaatg tacgaaagaa aatgtccagt
     2161 acttgcgcaa acttagaaag tggtttgagg gttatcctgt ttctatagaa ttgcgggatt
     2221 attcttggta cagcgatgaa tttcttgaac gtacaagaaa ctttatgtct agagccgctt
     2281 tttctttagc catcatcgat gagccacagt tgctgaatac aacgattcct tttgatccat
     2341 ttgtcaccaa tccgaacttt gctctttttc gattccatgg taggaatgcg caaggatggc
     2401 aagcaaatga taaagagtgg cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac
     2461 tgcaagaact agcacatgaa gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga
     2521 tttttaataa taattctggt ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat
     2581 taaatatcaa gccagatgga ctcaatccaa cccaaatgga tctgttttaa gggcttattt
     2641 tattcaagaa aggggcgaag tctatgatca aagctatttt ttttgatatt gacggaacat
     2701 tggtcaccaa tcgttcaaaa gcgttggaat caacaaaaca agcaattgaa tatgctcgga
     2761 aaaacggcat tttatgcggt gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca
     2821 ttgatgaatt ggaattagat atgtatgtcg tgtataatgg tcaactggtt tttactgcag
     2881 atcgcacgat tatcgaccac ccttttgaac aaaaagtatt ggaacatatt gttgaatttg
     2941 cagatgagaa ccatcgacaa atcgtttttg gtgcgagaaa tcggttagat ggatcaacga
     3001 cgatgctatt aggacaatcg atttttatca agcgactagt cagcttttta ccgcgtaaat
     3061 tcccggtacg tctgatgaaa aaaatcctcc aggtttttag ccctcaccga caaaaggatc
     3121 gttatgaaac gttagagata ttaaaagaac caatttatca atgtatattg ctcagtccgg
     3181 agtcagaaca gaaaaagtta gaacatcgct ttcccgagtg tacgtttcag cgttcaaata
     3241 cgtatactgt agatatcatc ccaaaaggtg gttcaaaatt actaggcatc caagctttcg
     3301 caaatgctgt cgaaattgaa atggaagaaa ttatggcatt cggcgatcat tacaatgata
     3361 tcgagatgct gaaaggtgta ggaattggag tggccatggg gaatgctcag atcgaagtaa
     3421 aacatgctgc agactttgtg actcaatcaa atgaagaaga tgggatatac gaaggactga
     3481 aacacttcgg tctgatttat tagtcaatga aagaaggaaa aaatcagtat ggaagcagag
     3541 acaccttttc aaaaagtaga aacattccac aaaacttttg atccaagaag accagagata
     3601 cccacggctt ttacagaaaa tgaagctcat acacgtgctg cgttcaaaat agaagaactt
     3661 gttgaatttg ttgcagctac ttcacctaat aaagaacaac tgcatgaagc tgttatgctg
     3721 ctgcacgaag cattagataa agcagaagaa aaaatacttg gtaaaacaca gtggggaagt
     3781 gatccgctgg ttgaacaagt agatgcactg acagatatcc tttactttac ttatggttct
     3841 ttttcattac taggaatagt gcctgatcga atattcgaaa tcgtccatca ggcaaatatg
     3901 gggaaattat ttccagatgg aaaacctcat tatgaccctg taacaaataa agtgttgaaa
     3961 cctgaaaact ggcagatgga atatgctcca gaacctaaaa tcgcggcaga aatccaacgt
     4021 cagattcaag aagcgattgc taagaagaaa tccgctgata taggcatgtg cacaaatgaa
     4081 aaagagtgat cagagtttca ctatttttgt tatctttata cgatggaaaa acaaaagatt
     4141 ttcgtttaag ttagtgaatg catgaaaata gtatgctata atctagaata gatgtctttt
     4201 gcacgagaga cgtattattt ggatttatgc caagcagaac gccatctgtt gcataaaaga
     4261 tgcatactga aaacggagga accataaatg ggaaataatg ttatcatcgg aatcgtcatt
     4321 gcaatcatta tagtcgccat tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac
     4381 caagatcgat tggatgcctt agaaaaacgc aaggaagaat tatttgactt gccagtgatt
     4441 gaagaagtcg atgatgttaa aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt
     4501 gaatggagtc aaaaatggaa tgacatatct acttcctcgt tcgctgaact agaaagtcaa
     4561 attttcgaag tggaaaacct caatgaaagt tttcggttct taaaaggaaa aaaagcagtt
     4621 gttgaagcag aagctacaat ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt
     4681 ctaaaggaat tgcgggaaac ggaagaacgc aactcactag ctgttcagca ggcacttgac
     4741 gtctatgaag aactgaaaga aaaagtcaga gaagacgagc atgaatacgg accagctctt
     4801 tttgaattgc aaaaacagat caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta
     4861 aacacttctg gagaccctgt tgaagcccgc gaggttttgg ataaagcaga aaaacatacg
     4921 tatgaagtgg aagctctgat gaaaaaaatt ccaccgctgt atgaggagct ccataccact
     4981 ttccctgaac aacttgaaga aatcagtgat acttacgaga aattgatgga agaacaatat
     5041 gtattcccag aagaagatct tgctgaagat atcgcaaaag taagtcgaag aattgaaaat
     5101 tcattagcca atcttgaaaa aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca
     5161 gctgatttga ttgattcgtt atatgatatc ttagaacggg aaatggaagc tcagagatat
     5221 gtaaaaacta accaatcaac gattgcagag tatatcaaac atacaacaaa gaataaccgt
     5281 caattattga ttgagctgga tcacacatct caaagctata cattgaacca caatgaaatt
     5341 ggccgtgtac gaggtttcca aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg
     5401 attccacaga tccgtcaaca cgaaattcct tattcagaag tgcgcacgtt ctataaaaca
     5461 gtcttcaaag tcttagaaga tatcgaaaag caacaagtag agatcgacga ctcattacat
     5521 gaattgagaa aaggcgaaaa agaagcgcaa gaaaagatcg atacattcga attcaaattg
     5581 cgcagtttga aacgttttgt agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta
     5641 gaattctttt ttgttgctac tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt
     5701 cgagtaaaca tggaagaggt caatcgttta gttgctctat gtgaagaaga tctagaatta
     5761 ttagataaga agacacatga tttagtagat gcagcagcat taacggagca aatgctccaa
     5821 tatgcaaatc gttatcgtca tacacacgaa gaagtgagag aagcaattga taaaagctac
     5881 tatttattca ataaagaata tcactatcaa gaagcattag acgaaatcgg taccgcatta
     5941 gagcgagtag aacctggtgc tttcaaacgc atcgaagatt tttattttaa tcatccagac
     6001 ttagtataaa gcaaaaaa
//
LOCUS       DACEPW010000139         5908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEPW010000139 DACEPW010000000
VERSION     DACEPW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.142"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            330..2165
                     /gene="tdc"
                     /locus_tag="IW819_002128"
     CDS             330..2165
                     /gene="tdc"
                     /locus_tag="IW819_002128"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1245223.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVFP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2470..3948
                     /locus_tag="IW819_002129"
     CDS             2470..3948
                     /locus_tag="IW819_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1245224.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3914..5245
                     /locus_tag="IW819_002130"
     CDS             3914..5245
                     /locus_tag="IW819_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1245225.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5359..>5908
                     /locus_tag="IW819_002131"
     CDS             5359..>5908
                     /locus_tag="IW819_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1245226.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEES"
BASE COUNT         1813 a          954 c         1263 g         1878 t
ORIGIN      
        1 cagataattc tattaaaaaa taaattgtga atttttaaca atataaaaaa ataaaaatat
       61 gtttagtgaa tgaaggacag tcattttatt tgtttgttcg ctttttttag gttatctatt
      121 ttagaagact gatttattga aaaattgttc gcttttttgc gttgaattat cagaaaaatc
      181 cgactttatt tatttttgtt tgtaaaaatc tacgtggatt tgatgttata aaaaaaaatt
      241 tctcattttt ggcgctatag tgaagaggtg ttacagataa acacatgtat ctgcaagaca
      301 ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat ggatatcaag gccgtcttta
      361 taggcgacaa agctgaaaat ggaccagttt ataaaatgtt gttgaacaag atggtagatg
      421 aacatcttgg atggagagaa aattatattc cttcagatat gcctgcaatt agcgaaggag
      481 ataagctcac tccagattat ctagccaccc gcgaccatat gatagaggtc ttggatgaag
      541 tcagtcagcg attacgcgca ggatcgatcc catggcattc agcaggccgt tactggggac
      601 aaatgaatgc tgaaacattg atgcctgctt tattagctta caactatgcg atgctgtgga
      661 acccaaataa tgttgcattg gaatcttcta tggcaacttc gcaaatggaa gcagaagtag
      721 gacaggattt tgcttcatta ttcaatatga cagacggatg gggacacatt gcagcagatg
      781 gttctattgc taatcttgaa ggattatggt acgcccgttg catcaaatcg attcctttag
      841 ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc tgagtgggaa cttttgaatt
      901 tatctgttga agaaattttg gaaatgaccg aaagctttac ggacgaagaa atggatgagg
      961 tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag attagggaaa tggctggtcc
     1021 ctcaaacaaa acactattct tggatgaagg cgttagatat ttgcggagtc ggcttagatc
     1081 agatggttgc tatcccagtt caagaggatt atcgaatgga tattaatgct ttggaaaaaa
     1141 caattcgtga attagctgga caaaaaatcc caattctagg tgtggttgct gtcgtaggaa
     1201 cgacagaaga aggtcaagta gacagtgtag ataaaatcgt ccaattaaga gaaaggttaa
     1261 aagatgaagg gatctatttc tatttacatg ttgatgccgc atatggcggc tatgcgcgtt
     1321 cactgtttct aaacgaagca ggagaatttg tgccgtatgc ttccttagct gaattctttg
     1381 aagaacatca tgttttccac cactgcgtaa cgattgacaa agaggtatat gaagggttca
     1441 gagctatttc agaagcggat tctgtcacga tagatcctca taagatgggc tatgtccctt
     1501 atgctgctgg aggaatcgta atcaaacata aaaatatgag aaatatcatt tcttatttcg
     1561 cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat gttaggagct tatattttgg
     1621 aaggatcaaa agctggagca accgccgccg cagtctggac agcacatcgt gtatttccat
     1681 tgaatgtgac cggatatggt caattgattg gggcttcaat cgaggcagct caaagattca
     1741 gagaatttct cgatcatctg acttttactg ttaaaggaaa aacaatcgaa gtctatccat
     1801 tgaatcatcc tgattttaat atggtcaatt gggtattcaa agaacaaggc tgtacagatt
     1861 tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg gtcttcttat atggatggcg
     1921 atgtttatgg tgaacggttt attacttctc atactacatt tacacaagaa gattacggtg
     1981 attctccgat tcgttttgtt gaaagaatgg gtctaacgaa agaagaatgg aaaaaagaac
     2041 agaaaatcac tctgctacgt gcggctatca tgactcctta tttgaatgat gatcgaattt
     2101 tcaattttta tacaaaaaaa atcgccaaag caatggaaaa gaaactaaac gaaatcatcc
     2161 aatagaactg atttttgtgt gaaagaataa caagagacat atggagaaca gacaattatt
     2221 tgcttcatca aaacaattgt atttccccct gtttttcata tattcctcgc caagatattc
     2281 ttttttataa aacgttctat aagttggatc gttcgtttca tgtatcaaac ccagttcact
     2341 aagatcgttc aatggatgtt tcggtgatac tgggttttgt actgaatcga tgaaaccttg
     2401 tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac atatgtcgct attgaaagaa
     2461 ggaataaaga tgaaagatac tggttcatca aagcaaatca cgaccatgac ctttatcgga
     2521 atgacctgcg cccttgtagc aagtgtacga aatattccag atgtagcagc aacgagctgg
     2581 acgatgattt tttacatgct ggttgcagta ttattgtacg cttttccaat ctcattgatc
     2641 tctggtgaat ttgctggaat gtttccccaa aaaggtggtc cagaactttg ggtatcgaat
     2701 gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt tatgggtcca aatgtttcct
     2761 gggatggtga tggtcgcttc agcactcgct ccgttgtttg gtaatattat cgataatgtg
     2821 ccgcttggtt tgaacagcaa atttacatta gttgtcattt tagtcgttta ttggatcatc
     2881 acttttttga atttgaaatt tgatatggca aaaatcggcg gaaaagtcgg tgtttggtta
     2941 ggattgtacg tgcctttgac gatgatgctc ttattagggt ttgctgcgtg gatcaaagtt
     3001 gggatcgtac caaccggaac attaggcagt ttttcatggg acaaattaat accagatact
     3061 acgactgcat cctcatttgt ttattttgcg ccgatcatgt ttatttttac tggtatcgaa
     3121 atgtcctctg tgtatatcac tcgattggaa aatccagtta aaacgtatat tcgcggagta
     3181 tttgccgcgt taatttttat atttttcgtc aatattttga atgctttagt tgtagcaaat
     3241 gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc aatctatttc gatttattgc
     3301 caaattcttg gtttgccaca tcttattgtg aatctgttta gtttacttgt cttcattggt
     3361 gtagcagtcc aattatctgc ttgggcctca ggaccagcaa agacagtgac agaaagtgct
     3421 agaaaaggtg cgtatccacc aaaattcaat ttttggaaga cgaatcaatt tgacgtatcc
     3481 aaatccgtta ttttgactca gtctgtcatt atctcaatct ttgcactttt ttatttgctg
     3541 attcctggcg tcaatcaagc atttttgatg ctcgtaaatt caactactgt tatctactgt
     3601 atcgtctatg tcatcatggg gatcgcagtt ttgagattgc gttatacaca tgcaaagttg
     3661 aatcgaccat ttagaattgg aaaaaaagag aaaaagagca atcttggagt ttggactgtc
     3721 gttattgttt tatttgcggc aatcggattt tctgtcggac tgacgatgaa agctggaaca
     3781 tggatcaatc taattgcagt cacagccatc agtgtcattt tgtttgtagt accactctgt
     3841 attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg ccatgctgct tgaaaaagaa
     3901 aaaggtgaac ttgatggaaa aaccggcaat gaaacggact ctaactaatc gaaatatcca
     3961 gttaatcgca ttaggtggcg caattggaac aggattattt ttaggttcag caaaggcaat
     4021 tgcatcagca gggccagctg ttattctcgt ttatttgata tgcggtttgg ctatttatgg
     4081 tttgatgcgg gccatgggtg atttattttt agcaaatagt acatttaact ctatcagtga
     4141 tttcgttgac tattatttag gcaaaaagtg ggcatttttt gtcaattgga cttattggct
     4201 ttgctggctt ggagctggac ttgcggaatt aacagcaata ggattgtata tccatttctg
     4261 gtttgtacat gtaccagctt tgataagcgg aggtagcgca ctaatgatct taatggcagt
     4321 caaccttgtt gcagtcaagt ggtttggtga attggaatct tttttctcaa tcatcaagat
     4381 cttggggatt ttccttttta tcttattggg tataggattg agtgtctcag attttcatac
     4441 ccagtctatc ggaatcaaag aaaattttgg acagatcgat gaattactac cgtttggctt
     4501 tgctggaatg gtagtctcat ttcctatggt tttattcagt tttgctggaa ttgaaatgat
     4561 cggcttaacc gttggcgaga caagtgatcc gcaaaagaat ttgaaaaaag caatcaactg
     4621 tctgccttgg cgtattctct ttttctatat aggaacgatt ttgtcgattt tatttgttgt
     4681 tccatggcat tgtttagatt taaaagaaag tcctttagtg actatgctgc aagtgatcca
     4741 ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg gttgcttctt tgtcctcagc
     4801 aaatagtgca gtctacagta ctagcaggat tttatatcaa acagcaaaag aaaaaagttt
     4861 gccccattgg ttttgtgcgc tttctaagaa aggcgtacct ttacacggta ttttaattac
     4921 aggaatgtta ttttttgccg gtctttgtct acattttttt gtagctgata gtcgattgtt
     4981 ttttcagctt ttggcaggca tgacaacgag ttgttttcta tttgtgtggt cttcgatctt
     5041 gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac actacacaaa gatataaaga
     5101 tcttgctttg ttggtgtttt ttacttcaat tgctttcagc ttgttattcg aaagcacgac
     5161 acgctttatt ccttttttat tggttatctg gtttgtattc ttaaattttg tatataaaag
     5221 aataagcaat agaaatatca gttaggccca gatacatttt gcaaaacgaa ttatttcaga
     5281 cccccctgaa ataatcgata cgcctgaaaa aacttccctg cgtataataa cagtatcaat
     5341 taaacaggag gggataggat gaactggtac agattgacgg tagaagatgt attgaaaaac
     5401 tatcaaacat caacagaagg cttgtcgcaa gaagaacgac agaaacgcct gactgatcaa
     5461 ggatataatg aaataaaagc aaggcagcag acaaaacgat ggaaaaaaat agcaaagcat
     5521 tttactgatc tgctgatgat cgtgttgatc attgcctctt ttttgaaatt tgcttctagt
     5581 gaatatattg aaggaagtat tattttattc gtagtgatcg tcaatggact cgttagttac
     5641 tggcaagaaa gaaaagcgga agaatcattg aatggtttga agcagctgat gggacaagaa
     5701 gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg cgaaagaact ggtaaaagga
     5761 gatgtcattg tcttacgtgc cggagatgtc gttccagcag atatccgatt aatcgaagca
     5821 catgatttat tagtggaaga atcgattttg acgggagaat cagaagcatc tgaaaaaagt
     5881 catgccgttt tgacagaaga agaatcga
//
LOCUS       DACEPW010000140         5745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEPW010000140 DACEPW010000000
VERSION     DACEPW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.143"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..213
                     /locus_tag="IW819_002132"
     CDS             <1..213
                     /locus_tag="IW819_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HAQ1245227.1"
                     /translation="SKNIAVWFFFGAVVMAYLELPLIAIAAVGTIIAIVIAQRDLQLK
                     NISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            210..1031
                     /locus_tag="IW819_002133"
     CDS             210..1031
                     /locus_tag="IW819_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1245228.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            1082..3181
                     /locus_tag="IW819_002134"
     CDS             1082..3181
                     /locus_tag="IW819_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1245229.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            3178..4296
                     /locus_tag="IW819_002135"
     CDS             3178..4296
                     /locus_tag="IW819_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1245230.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            4335..5213
                     /locus_tag="IW819_002136"
     CDS             4335..5213
                     /locus_tag="IW819_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1245231.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            5404..5556
                     /locus_tag="IW819_002137"
     CDS             5404..5556
                     /locus_tag="IW819_002137"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245232.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         1719 a          976 c         1291 g         1759 t
ORIGIN      
        1 agtaaaaata ttgctgtgtg gtttttcttt ggggccgttg ttatggcgta tctcgagtta
       61 ccattgattg caattgccgc agttggaacg attattgcaa ttgttatagc ccaaagagat
      121 ctacaactaa aaaatatatc tgttcatgca gttcaaagcg atgctgctgt aaatgcaacg
      181 cttgaccaag aagaggagga tttctttcaa tgaaattagc tgatggaatt tctaaagatg
      241 agaaaaaatt ggttcggaag atgttttggc gctcgatgac tatgtatgct tcggtgaatc
      301 cggtgacgat gggcggcggt ggtttcgcct attctatgat gcccttcatt aatcatttct
      361 acaaaagtga agaagatcga gctgcggcgc tggaacgtca tacacagtat ttttcgacga
      421 ccattccttg cgcgtccttt gtcatgggga ttgcggcatc gatggagaag gaaaattctg
      481 aaaaagctgg ttttgacgtg gaaagtatca acgcgatcaa aacgagtttg atgggaccat
      541 tggctggaat tggcgattca ttgttctggg gagtttggcg tgtcgttacc gcgggactag
      601 cgatcaactt agccaatgcc ggaaatattt tggcaccaat tatcttttta gttatgttca
      661 atatacctaa ttacttgatg cgatactatg gcggcgtttt aggctattca ttaggctcta
      721 aatatattga aaagctgtat gcgaacgggt taattgaaat tctgacaaaa gctgctagta
      781 tcttgggtct gatcatggta ggtgcaatga cgatcagtat ggtcaaattc aataccgttt
      841 tgagcttttc aatgaagggc gaagaactaa tgaatgtcca agaaatcctc gatagtattt
      901 ttaaaggaat tgtaccgtta ttggtaacat tgagctgttt caaattacta gataaaaaag
      961 tcggcgtcat caaaattatt tttggaatgg tcgtcttggg aattttattg tcattacttg
     1021 gaatcgcata gtaggaagct gcagctacta ggctgcagtt tttttagcaa ggagggaaaa
     1081 gatgactatt aatgaagtaa tttattcacc caaacaccca ttttttaatg ctgagttaaa
     1141 cgacggaaat gtgactactt caaacgaata cagctactat cggaatattc ctgatgtaaa
     1201 gggattgacc ttcgatgtaa gtaatcaaaa gacattagca aaccttgatg tttgcgggac
     1261 gattcaaacg ttgacctttt atcgagaaaa tttactaact gaggaaaaac caggtgtttg
     1321 ggtcaataaa cagctctctc aaaccaattc gttatcctta aaggttgaag taaacgggat
     1381 tgtctaccat ttgtcagaat cggatcatcg tattgaagtc gatgtgattc aagactgttt
     1441 gcctagatat attcatcatt attcgacgtt taaggtcatt gtagtatcct ttgctcccat
     1501 actggctaat aagcgtttaa gtatgcttgt tcaacaggtt tacattgtga atgaaacaca
     1561 cgaaagcctt caagtttcag tgaaagatgt gccttcgtat caagagaagt attcggatca
     1621 gcaaaatgtg ttgatttctc aaaaaggaaa taaggacacg atcgcacaac aggaagtggc
     1681 aagcttcgcc attgcttttg tggacccaaa tgcttttgaa gaagtaatga cttttcagga
     1741 atctgatatc gaaaaatggc tgaaggaaac attgaactat tttgctcagc tatttgggca
     1801 gctgtcaatg ccaaacgata gagttgtcca tctctatcgt cgtgcacttt atcaaagctt
     1861 ttctagtttc ggtatgaatc gacagggaca ggtcgtcggc agcaattggg gcagctatcc
     1921 tgctactaat cgaatttgga ataaggatat gtattacgca tcattgccat tcaccatgtt
     1981 tgaaccggag ttatgtcaaa aaacggttct atggtttgat caatatggcg ttaagtttcc
     2041 tggttcaaaa tttacaggag gtatcaatca ttctctgagt aattcgttat ccagtgcgtt
     2101 actggctagt ctttattttg aacatactgg ggatacaaaa ttctttgagc ggtgtccgca
     2161 ggttctactg aatgcttcaa gaattattga ggacatatta gctcaacgtc ggtcagggga
     2221 accaatgctt tttccatctg tttggttatc agacgccttt gcacttggaa agtatcatac
     2281 cggcagcaat ctttgtcttt ggaaggcctg tattggactt gcagaaattt ttgaagccct
     2341 agaacaactg agtcttagca agcgatacaa aaacattgcc tgtaaactaa aggaatcaat
     2401 ttgtaaacaa atgactatcg atggtccttt tggtgagcaa tttttggagg gaatcggtga
     2461 tgaggagaaa acaacctatt cagtgaagca ctatcaaaaa ccgattcttg agcaagggtt
     2521 aatcttttta agtgatgtga ttgtggatgg aaaaattaat ttattgatgc atgatggaga
     2581 agaatcagac actacgttga tgccttttta tcaattttta gataataaag atcatttata
     2641 tcagaacacc atggcctttt ctgctagttc tttcaatcca acctatagcg aggaaatcaa
     2701 aggcatcact tggggattag aatccggcgc cacttttcct ggctttatca ctgttttaat
     2761 gagcgagctg cacaaccctc aagctttcgt aacccgatta gaagaactaa tttgtttggc
     2821 agatttagac ggttcgtggt ggtggtggcc gtatcgctta gaggctcaac aaggagaagt
     2881 cgtgcgagac tttggctgcg gcaaatgcgg ctgggcatcg ggtctttttg tcagcctaat
     2941 gatcagtcaa tatttcggat tgaaattcaa aaaaggagta ttgcaaattg atcctgttga
     3001 taatttaacg ttttcttgga agaatttgaa gttcggtcaa gccttcattt cgattgaatg
     3061 tacgcaatcc gagttgagta tttcaaattt gtcagaaaag ccattgaaaa taagtgatac
     3121 gaaaaaaatt cttcacgtag aaaagggaaa aacgattcac attcaaagga gaaaaagatg
     3181 agtatactaa aaaatattac ccaatttaaa aaaatccaga gtcgagcaat cactcccgaa
     3241 aatccaacag gcgagaaagg taaagctgcg atgttgccaa gcgagctagg accaagccgc
     3301 aaaggtcgtg gcgcaattcc gctgacttct ggtgaagaaa ctgtgattgc ggatattact
     3361 ggaacgggaa taattcgtca tatgtggatg acgattcgtg atcgaacaga atttggcagt
     3421 tttgttttac gtgatttaat cttaagaatt tattgggatg ggtcggatag ccctgctgtt
     3481 gaaagtcctt taggggattt cttttgtaat gggtttggag agcgttgcga ggtgaactcg
     3541 ttgccgatag tggttaatcc tacagggggc tttaattcct atttcgaaat gcctttccaa
     3601 acatccgcta aaattacgat cgagaattta catccgaagg atatgtcctc atttttttat
     3661 acgataaatt acgctttggt ggacgaattg gaagaaaata gcctatattt tcacgcaaat
     3721 tggcatcggg aacgcattac caaattacag gaggattata agttaattga tcgattaaag
     3781 ggtcaaggct attacgtggg tacttacctt gcgcttacct gcctagaacg ttattggtgg
     3841 ggcgaaggcg agtttaaatt ctacattgat ggggatagtg agtatccgac cgtaacctca
     3901 acaggttcag aagattattt tggcggggcg tgggcctttc atgagtacga tgagttcgga
     3961 cgtccacatg cgaaaaacta ttcaacagcc tttttagggt atccttacca atcaaataga
     4021 gatgccacaa gagatcgttt tgaaacggga aaattgaatg ccgttcatgc ctttggtgac
     4081 gacgcaatgc cacgacatgg tctttatcgt tggcatcttc aagaccccat ctgttttacc
     4141 gaagatttat cggtcacttt acaacaaatc ggcaacgatg atgttcgctt atttgagcgg
     4201 caagacgatg ttgctagtgt cgcttactgg tattcaacag aagcttcagg aaatactaaa
     4261 cgtcatttca ctgatcgaga acgcttgccg cgttaatgaa gtgtttttca catattcgaa
     4321 gggaggaacc tatgatgata tatggggcaa ttgaagctgg agggactaaa tttgtctgtg
     4381 cagtcggtac tgaggaatta acgattttgg aaagagtcag ttttcccaca acaactccag
     4441 ttgaaacaat gaagcaggtc attgattttt ttaaaccttt taaggaacaa ttggcaggca
     4501 tcggggtcgg ctgctttggt ccaattgata ttcaatgcaa atcaacgagt ttcggtcata
     4561 tcacatcaac accaaaactt gcttggcaaa atttcaattt tgtaggaacg ctaaagcaat
     4621 atttcaatat tccgattgaa tggacaacag atgtcaatgc ggcttgctat ggtgaatatg
     4681 ttgctggttc tggcaaaggg ttagcaggtg ttgcatactt cactgtggga acaggtatcg
     4741 gaggtggtgc cttgttcaat gggactttcg tggaagggtt cagtcatccg gaaatggggc
     4801 atatgttgat caagcgacat ccaaaggaca atttttcagg aaactgtccc ttccaccaag
     4861 attgtttaga ggggatggca gcaggaccag cgattgaaaa acggttgggt gtaaaaggcc
     4921 agaatttatt ggctgacgat tcgttttggc agattgaagc cttttacctt gcccaatgtg
     4981 catacaatac aacactgatg ttttcgccag atagaattat ttttggcggt ggtgtaatga
     5041 aacaagaaca catgaagaaa aaagttcagg ataaatttgt tgagcttata aatggatatg
     5101 ttgaaatacc accaattgat agctacatta ttacgcccga attaggtgat aatgccggaa
     5161 taatcggcgg attagcattg gccagaaaag cggtaagaaa taagcaacca taattaatga
     5221 atgtttcatt gatactcatg tcaaaagtat ttaaccgagt aatcaaatga tcaaatgaag
     5281 ttggataaag cgaaaatgaa ttcaaataaa aaggctctaa gtcaaatcgt gttgttttca
     5341 ttaaatcact gaaaggaaat gcttctgcgt ttcctttttt ctttttgttg tgcgctgtat
     5401 accttgagga tattcactgg tggcgtacac ttaaatagta atatattgtg ttactttatg
     5461 tctgtggtaa tagttatagt tgcttaccaa tgtatgttca tttttcgaaa tgttgacttc
     5521 ataatccttg gcttaatgtt tgaatttttt tggtgataga ttttttcagc cgtataaaat
     5581 aaatcgtaat tctattatag agagttgtga ttataaaaag atagcaagaa aaaaaaagaa
     5641 agtttctata cattttaaat attttattcc tatttctatt gctaaaaaag tagaaattct
     5701 atatttacct ttaactctcg cttctcttgc aaatgtagtt agcat
//
LOCUS       DACEPW010000141         5702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEPW010000141 DACEPW010000000
VERSION     DACEPW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.144"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(120..1370)
                     /locus_tag="IW819_002138"
     CDS             complement(120..1370)
                     /locus_tag="IW819_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1245233.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
     gene            1780..2313
                     /locus_tag="IW819_002139"
     CDS             1780..2313
                     /locus_tag="IW819_002139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245234.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKIQPFIF"
     gene            2340..4151
                     /locus_tag="IW819_002140"
     CDS             2340..4151
                     /locus_tag="IW819_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1245235.1"
                     /translation="MNENGGFEESKKYQATSEEEKAAVRELLEEAAPTDSLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPEDKKR
                     IESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAENKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            4212..5681
                     /locus_tag="IW819_002141"
     CDS             4212..5681
                     /locus_tag="IW819_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ1245236.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
BASE COUNT         2079 a          935 c         1199 g         1489 t
ORIGIN      
        1 caagatgaaa tttaagattt tctttattga gtaatatgtt ttgaagcgca cgaaactaga
       61 cccgatcaaa atcaaatttt tttcgggttg attcaaggat ttgcgtcaat aacggattgt
      121 tatttctcta tcacactcct tggtggcgta tagagttggt gtttacgtag tagcgtatcc
      181 accagacgcg taaattttct tgcggttagg acgagtgcac gtttgtgttg atgctttgga
      241 acctccttat atttactttg ataaaaagct ttgtattcag gaagataatt ttttacagag
      301 ttggcagctt caactaagta gtatctaaaa tatcgatttc cttgttttgt cagaggtgta
      361 ttttgcgact gatagtttcc agattggttc actttccaac ttaatccagc gtattttgcc
      421 aattttgtct gatcttcaaa tctttcgatt tgaccaattt cagcgattaa accggcagca
      481 tagactttac caacgcctgg aatactggtt aagcattgat attctggaat gacaacaatt
      541 acttgttcga tggctttatc taattctttg atattttttt caagtgctcg tatttctcgt
      601 acgagaacac ttaaaacaac attaattgat tcttgtgcaa gagcacctag gcggtaactc
      661 atggtaattg cacgttgaat tgctttagcg attttttctg gttgcttgaa acggcctttt
      721 cctttttctt gaagtaaatc acagaaagct tctaaaggaa gctcagcaag ttcatccaat
      781 gtgaaatctt ccgtcatgag tgaaatgagg gttgcactaa aaagactcgt cgattcgtct
      841 cgcatttctc gagcaagcgt attacatttg tacgttagat tttcaagaaa atgttgcttt
      901 gtacgaatca attgcttaat aagttgatag cgagtacgtg ttaaacgctg taaggccatg
      961 tatttctctt ctttgatagg ggaagttgtg aatcgttgaa tacgcaagaa atcagcgatt
     1021 cttaatgcat cgtttcgatc agttttattc tcatcaaaca tgcgactaaa ttgtttcacc
     1081 cgaaaaggat tttcaatcgt tacaagtgtg ttgagcttct ttagtttctc atcttcatga
     1141 aaaaacatgg aaggatggaa gctgtacatg gacgttgact ccatgccaat cacaatttga
     1201 tcaaagtggt agctgtcatt gaattcaaga attgtttctc gtatgaatga agcaccttcg
     1261 atgtcattgg caacagagat ttcagataag atagacaatt gattgtcgtc tgttaaaaaa
     1321 caaacatcta gtttttcaga gctaacgtca ataccgacaa ataatttcat ggaagaagga
     1381 cctcctttcg atataaattt tggaaaatgc ttcgatactg tgggatttcc cagaaactca
     1441 ctgtatagcc acaacctcgc ctaatagaat acggtgataa aagagctaga agctgccttc
     1501 caaactacta tttgaaagtc tagtccatta tcgaaacagc taatgagttg gtagtgataa
     1561 cagtggttcg ggtaacagac taaacagggt agacaaacgc tacaggagga aatagcaaat
     1621 tcccgttgga tcctttccat acatccattg tactctgaga aatcacacaa tatcgaaaca
     1681 aattccgtta ggaatgtgtt attcaataaa ttttataatc tagaaacatt ttgaataaaa
     1741 actattagac aaatgatatt ttacgaggag gaaacggaca tggcagaaga aaaagaacca
     1801 ctagacaata gtcgcttggg aaaatccaaa agaaaattag tacgattgca aaatgaatta
     1861 aacgaacaaa ttgagaagat gtttgaacac cagagaaaga ccaacggtca gccaatgaac
     1921 gataagagaa atgggcattc ttggtttagg caacaagaac gtttagaaaa caaagtgcat
     1981 agtctgagag aagagatcaa gcaacaagaa aaacaggtag aaaaacttga aagacaagaa
     2041 gagctaaagg aaatgggcta taacaagtac ggcggcctgg atatgacaat cgaaaatatt
     2101 ccaagaatca aagaagaaat cgaacgcttt gaaaaaggtg aatcgacttt ttcagccgct
     2161 acgatccgaa aataccaacg gaaattggaa acattagaac agctgaaaga aagatcagaa
     2221 aaaggaaaag aaaatcttct tcctgaagta caagcaatta ttgacagcgg acgagtgacg
     2281 caatggaaaa aaatccaacc atttattttt tgaaagacta tcgaaaagtt gctttagaac
     2341 tgaatgaaaa cgggggattt gaggaatcaa aaaaatacca agccacgtcg gaagaggaaa
     2401 aagcagctgt tcgtgaatta cttgaagagg ctgcgccaac tgattctttg aaggagaaag
     2461 aaaacgatca tttctatcga gtagaattca acgaaaatga tccagaaaaa ggattgaaaa
     2521 gttatggtgg ggagatcgta acattagaat tgatcgatca actcaaaacg ttggataacc
     2581 aatacgccgc agaagcaggt tattataaat tctattttga agaagtgaag aacggagaag
     2641 tagtcgatca gatccgaatg gatctagggg atggcgtcaa cagtaatcaa cccatctatt
     2701 tagagttagc aaaattgtgc gaacaagtac ctgaaaagga acaaaaacaa acggtcaaaa
     2761 atgaacctgt ttcgaaagaa caaatgcagt cgaaagaaac aaaaacaaaa caaaagggct
     2821 atcaaaaacg aacggcagaa gagattaagc aagaagtcaa atccctttcg caacaggcac
     2881 tggatgcggt gaaaaagcat acccattctc ctgaagacgt gaaagaactt ttgaatttta
     2941 tgtctcgttt tccagaacga tcctttagaa atcaattatt gattgaggaa caatttccag
     3001 gtgcaacggc ttgtttaggt cgtgctgcgt tgaaaaaaga aggaatctat atcaaaaaag
     3061 gggaacaagg gaacaagatc tttgtcagaa aaacggtcaa aggattttat gagaaaggac
     3121 gtggctttgt tcgagaaaca gaagctacac cagaagataa aaaacgaatc gaaagtggtc
     3181 aaatcgaagt gatcaaaaaa ccttactaca cgattgaaaa agtgtttgat atcacacaaa
     3241 cacagttaaa acctgaagac tatccgaaaa tctttccgaa ccgtgtcttt gattttcaat
     3301 tggataaaaa aggacagtca gaacttcaag ctgggattca agcggtagcc aacaatattg
     3361 gaatacaaat tcgagatatg acagaaagtg aagtctatca aagagaatta ggacaagcac
     3421 ggggggcgta tgttcgtaac gaatttactg gcgaagaaga aatcgtcatg aatactcgaa
     3481 atactcctac gcagcattta gctacaagta ttcatgaatt agcccatgca agaatgcaca
     3541 aattttcaga attagatacc gcaactaaag aattgcaggc agaaatgact tcttatattg
     3601 tgtgcaaaca ttttggaatg gacacttcgg agaaagctat tccttatatt gcagcatgga
     3661 cgaagaatgg tcagacgttg gatgataagg aagcggcgga acgtgggaaa atcatgaatg
     3721 atgtcagccg tgtcgcaaat gaattcattc aaaccatttc taatgaaatc aatcaataca
     3781 gagaaagaga accagagatc caaccagcag aagtgaagca ggagcataaa aatactgtag
     3841 aaacggcaga gaataagaat attgtgcccg ttggttcact ttggatcgac aagaaagacg
     3901 ggaaagtaat ggaagaagat acgggtcgaa cgttacattt gaaggatgcg gcgtttattg
     3961 aagatccgca gcataatcaa gtgattttaa aaggaaaaag ttatggaaaa gagattcaaa
     4021 cggcgttacc ttcagaagaa tttgcgctag gaaatccatt ccgattttca cttgaaacag
     4081 tgtcagaagt ggccaaacga aaaaatgaaa tctattatga acaacaattg tcacaaaaag
     4141 aacgcgtata aaacaagtaa aaaagactat ctgaaaaacg gatagtcttt tttcttttgg
     4201 gaggtttgaa aatgaccgaa aaaaaaatga gtcttattga tcgatgtaaa cagatcgata
     4261 tagtggattt tgctcgaaac aatggaatgg ctgtagtcaa taaaggacga gactatcgct
     4321 tagaagacca tgattcattt gtttttgatc gaagaaaaca acgtttttat tggaacagtc
     4381 aaaatattag tggcgacatt attgaattag ctaaactttt tttcatagac aaagagatcc
     4441 aagactctaa gcaacaattt aaagcagcca cagattttat tctaaagaat gaagacaaaa
     4501 cagaacgagt agaaaatctt cattttgaga cagaaaagta caaagatcac cccgttgatt
     4561 atcaaccact cacagaaaaa ggaaggaatt atctgaagga agaacgaaaa ctaccagagt
     4621 ggctgattga ttatgcagaa aaagaagggt tgattgctga actaaagccg aaacatgaac
     4681 ggcaaaattt tttagttgga gacgaccgtt tagatcatgc ggttgctttt ttatggaaag
     4741 atccacagac aggagagaca gtaggggcaa gttatcaagg aacgatcgtc gattttaatc
     4801 gtttcggtaa acggggaacg tataaacaca ttgacaagaa tcctacgcca aatcacggat
     4861 ttaatttaaa aataggcgat ccgaagcatt taaaattctt tgaaagtagt attgatctgt
     4921 taagttacgc tgcattgaat cgggagaaac tacaagatgc ctggttagtt tcgatggacg
     4981 ggttaaaaca tcatgtaatc agtcattacg ttgaagagtc gatttctgaa ttaagacgaa
     5041 aacaaacatt tcctcaatcg attgaaatat gtgtggacaa tgatcgtgca ggacatattt
     5101 tttatgaaaa agaacagatg aagggaatcg ttgatccttt taccaataag aaaatccgtt
     5161 gtgagcgagg aattccaaat gattggcaag tacctaaaga atacaaagct acttatgaag
     5221 cagtcgcaaa agaaatgagt gttgaacctg aagcaattat ggccatacac aaaactgaaa
     5281 ctaatttaca gctgacaaat caattggtgt ctgctcatga tgttcaatct acgtttggaa
     5341 agatgttggc gaagggcgaa ccagtagaaa caattgattt gaaagaagca tgtaccacgg
     5401 tcgcgaagga actgaaagta tgtgaacgcg cagatggaac atacaacttt gatcgttttt
     5461 atagtagaaa agccaacata aaagacgtta atgcaggaat ccttctttcc tataaagcag
     5521 aacaatacta caaaggctat aaaaagcatg aacatgaatt tgtacctgaa gtgaaaaagg
     5581 attggaatga tcaattgaag catgagatcc agcaacaaga aatcagaaag caaaaacgtg
     5641 cgatgttgtt tcaacaaggc agacaacaag aaagggagtg aagtcgtcac tatgaaaacc
     5701 gt
//
LOCUS       DACEPW010000142         5550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEPW010000142 DACEPW010000000
VERSION     DACEPW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.145"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..573
                     /locus_tag="IW819_002142"
     CDS             <1..573
                     /locus_tag="IW819_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1245237.1"
                     /translation="NRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSNET
                     YEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDPNT
                     SPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRKLF
                     SEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(595..1749)
                     /locus_tag="IW819_002143"
     CDS             complement(595..1749)
                     /locus_tag="IW819_002143"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1245238.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1934..4375
                     /locus_tag="IW819_002144"
     CDS             1934..4375
                     /locus_tag="IW819_002144"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1245239.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4705..5409)
                     /locus_tag="IW819_002145"
     CDS             complement(4705..5409)
                     /locus_tag="IW819_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245240.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1775 a         1006 c         1149 g         1620 t
ORIGIN      
        1 aatcgctatg atttgatcga tttttcttca ggaatgctag aaaatgcaaa aaaagaagca
       61 gaaaaaaatg agttgccaaa tgtttccttt atcaaacagt cgttagaaga atttatccaa
      121 tcaaatgaga catatgaaat ggtttttaca gcagcaaatc cagcattggt acatcttatt
      181 caattagaaa aattgatgaa aaagtcaagc aaatggtgcg tgataatcag agtagtggaa
      241 tcaatcgatg atgtttttta tcctatcgaa aagcaactag gaatgataga agaagatcct
      301 aatacatcgc cagttgtaat ggatcaattt gaaacatact taattgatga aggattcaaa
      361 gtgaagaaaa aagattttac ttattttaca caggaatcaa ttactgaaga attgatgaag
      421 ctatactatg acgcgtacgc aaaagatcct gttgtgacca ggtacattcg caaactattc
      481 agtgaaaaac gagaaatcct tagtacgact aagctgacct atagactgct cctttggtcc
      541 gcagataaaa aaagaacctt tcacgactcg tgaagggttc tttttatgat tttattatat
      601 tttgtagtaa cgaatatcat tttcgatctt tgtaatcgaa aaaggtacat ctgaagtata
      661 ttctgcacat tctccgtgtt caggaagcgt ctcactgaaa atcgtttcat attcgtcaac
      721 agtgatccgt gttcgagaat ctagcatctc ttgatgagat tgtgcgaaga gatattcttg
      781 atagttttct tccagataac ctgtaaaaaa ttcacttact gcaccagaac cgtagctgaa
      841 tagaccgatg cgatctcctg gctgtagtga tttcgaattt tccagtagag aagttagccc
      901 caggtataat gaaccagtgt aaagattacc gattcgtcgg ctgtaacgga tgctttcttc
      961 atagcgagcc ataagacgtt cctgattttc ttcgtctgtt tggtctaata cgctttgcaa
     1021 tgcctttttt cccatcttag tatacggaat atggaaagca atcgcctgat aatcttcgag
     1081 tcctcgaccc gacaattctt tatgtcgatt ccaaactttt tggaatgatt cgatatacgt
     1141 agaattagat aaaggaccat caacaacagg aaattcgcta taatctggac gccagaaatc
     1201 atagatatct tctgtcagaa atacgctgtc gtcttcaatc gataaaatac gcgggttttg
     1261 agtgatcatc atcgcaacag caccgacacc ttgcgtcact tcaccaccgc ttgccaagcc
     1321 gtaacgagca atatcacttg ctatgactag tacttttcgt tctggatgat ttttgacata
     1381 ttctttcgcc atgtgcaaag ctgcggtgcc accgtagcaa gcctctttta cttcaaaaga
     1441 acgagcaaaa gattggattt tcagtaaatg gtggataatt accgcactcg cttttgaatg
     1501 atcaatcccg gattctgttc cgacgatgac catgtcgatt agctcacggt ctttttctgt
     1561 tacgattttg ctggcagcat ttgctcctag tgtaatgatg tcttcatttg cacgattgac
     1621 tgccatttgg tcttgaccaa tccctatatg gtattttgca ggatcatccc cacggctttc
     1681 tgccaactct gtcatatcta aatataaatt aggaataaaa aaggaaagac gatcaatccc
     1741 tattttcatt tcaacacctc tttacttatg ataaaaaact tactttacta aggtatcata
     1801 aaaagattaa aagaaatata aaaaagagaa tatttaagga ttgttaaaat ttaatcttag
     1861 aaaaaaataa ctggaacaag agctatttgt ttttacagca aagaatggta tatttttgta
     1921 ggaggtggct tacttgaaag aagtcgttat gatagatgct gcaagaacgc cgataggaaa
     1981 gtatcgcgga agtttaagtc catttactgc agttgaatta ggcacacttg tgactaaagg
     2041 attattagat aaaacaaaac tgaaaaaaga taaaatcgat caagtaatat ttggaaatgt
     2101 cctgcaagct ggtaatggtc aaaatgttgc cagacagatt gctttgaaca gcggtctccc
     2161 tgtagatgtc ccagcaatga cgatcaatga agtttgcgga tctggtatga aagcagtgat
     2221 cttagcacgc cagctgatcc aattaggtga agcagaactt gtgatagccg gcggaacaga
     2281 gagtatgtct caagcaccta tgctgaaaca gtatcagtca gaaacaaatg aatatggtga
     2341 accaatttcc agtatggtca acgacggatt gactgacgca ttttcaaatg cacatatggg
     2401 attaaccgca gagaatgttg caacacaatt ttctgtgagc agagaagagc aggatcgcta
     2461 tgccttgtcg tcccagttga aagcagcaca tgctgtcgaa gccggtgtat tttctgagga
     2521 gatcatccca gtcaagattt ctgatgaaga cgtgttatct gaggatgaag cagttcgtgg
     2581 aaatagtaca ttggaaaaac tgggcacgtt acgtacagta ttctcagaag aaggaactgt
     2641 aacagcagga aatgcttccc cgttgaatga cggtgcctct gtggtgatcc ttgcatccaa
     2701 agaatacgca gaaaataata atctgcctta tttagcaacc atcaaagaag tagcggaggt
     2761 cggtattgac ccaagtatca tgggaatcgc tccaatcaaa gccattcaaa aattgacaga
     2821 tagatcagga atgaatttat caacaatcga tctttttgag atcaatgaag catttgcagc
     2881 ttcttctatc gtagttagcc aagaactgca gttagatgaa gaaaaggtca atatatatgg
     2941 aggagcaatc gcattaggcc atccaattgg tgcaagcggt gcacgtatct tgactacatt
     3001 agcttacgga ttgcttcgtg aacagaagag atacggtatt gcttcattat gtattggtgg
     3061 aggtttaggc ttggcagtct tgttagaagc gaatatggag caaacacata aggatgtgca
     3121 aaaaaaaaag ttttatcagc ttacgccatc tgaacggcgc agtcaattaa tagaaaaaaa
     3181 tgtattgaca caagaaactg cgttgatttt tcaggaacaa acactttctg aagagctctc
     3241 cgatcacatg atcgaaaacc aagtgagtga agtggaaatc ccaatgggga tcgcgcagaa
     3301 ttttcaaatc aatggcaaga aaaaatggat tccgatggca acagaggaac catctgtcat
     3361 tgcagctgca agtaatggtg caaaaatctg tggaaatatc tgcgcagaaa cacctcagcg
     3421 gctgatgcga gggcaaatcg ttctatcagg aaaatcggag tatcaagcag tgatcaatgc
     3481 agttaatcat cgaaaagaag agctgattct ttgtgcaaat gaaagttatc cttcaatcgt
     3541 gaaacgcggg ggcggtgttc aagatatctc tactcgggaa ttcatgggga gtttccatgc
     3601 atacttatct attgattttt tagtggatgt aaaagatgca atgggcgcta acatgatcaa
     3661 ctcgattctt gagagtgtgg caaacaagtt acgggagtgg ttccctgaag aagagattct
     3721 attcagtatt ttaagtaatt ttgctacaga atcattggct tctgcctgtt gtgaaatccc
     3781 attcgaacgc ttagggagaa acaaagaaat cggtgaacaa attgccaaaa aaatccaaca
     3841 ggcaggggaa tatgcgaagc ttgaccctta ccgtgcagca acgcacaaca aagggatcat
     3901 gaatggtata gaagctgtag tggcagctac aggaaatgat acacgtgcgg ttagcgcttc
     3961 gatccacgcc tacgcggcaa gaaatggatt ataccaagga ttaacagact ggcagatcaa
     4021 aggggataaa ctagttggaa aactaacggt gcctttggct gttgccacag taggcggagc
     4081 ttccaatatc ctgccaaaag caaaagcatc tttagctatg ttagatattg attcagccaa
     4141 ggaactagca caagtaattg ctgcagtagg gttagctcaa aacctggctg ctttgcgtgc
     4201 attagttaca gaaggaatcc aaaaaggcca catgggactt caagctcgtt cattagcgat
     4261 ctcaattggg gctattggtg aggaaatcga gcaagttgcg aaaaaattac gtgaagcaga
     4321 aaagatgaac cagcagacag ctatacaaat attggaaaaa atccgggaaa aataaaaaag
     4381 caatatcttt agaagaataa agcaagattt acagttattt tatttactgc agatcttgct
     4441 tttttctcta tcagaaaata tgtttattat tagaattttt tatagttaag gttaggcatc
     4501 ttaagaaatc aggcaactcc aagatatctt caggtagaag agaagtcgcc taacattgta
     4561 ggatcatagg tgatttgttg gacaggtcaa gctgatattt gagcctgatg ttagaaggaa
     4621 tttcttttta cttgctgtag aaattattat atgactaaaa aggctgggat tttaaatccc
     4681 agccttttat gacaaataga atttttatcg atcagtaatc gctgtcgctg caaattgttc
     4741 gatcaaagga atcaaagtca atttgacttt gtatttccgt tcgagtgctt tttcgataac
     4801 gaactcattt tcagtagtca tagaaatagt tgctaattta cgaggttcat cagcattttt
     4861 ttcattaata actacttcat gttcattttg ttctgtgtaa aatttaggat actcagcaac
     4921 ggcttttgaa aaatcagcaa aacttaaagc ggcattcgta tcagttgagc gtgtattttc
     4981 agttgtgtcc gcttttggtt tttccgtttt ttcttctggc acactgactg tttcttctgt
     5041 aactggtgtc gcaacagctt gcgcagttgg ttcatcgttt ttcaatcgtt gatcattcca
     5101 gtaagacacg atttcaataa gaagattgtc acggaatcgt tcgaaaatcc cgttttctcc
     5161 tgaacgaatt ctctttaata cagtattgaa gttgtcatcg attggcacgt tgatccgtgt
     5221 atcttcattt gttttcccag ctaatccgat cgttaagatt tgacggtttt tgggatcaag
     5281 catgacttgt aacggcaaac cataacgtcg caagctctct tcttctagag aacggaataa
     5341 ttgtcctctt acaccatcca atgaaggacg gaattgattt aataattttt gtttctgttt
     5401 tttcttcata tgttctctcc ttcgctcata ccctttcgat tataagaaaa aaacagccaa
     5461 atgaaaagga gtaatgcaaa aaaacttgcg tatacgcaaa ttacaacaga aaaatttgcg
     5521 tattttcccg ttttttgaaa gaaaaataaa
//
LOCUS       DACEPW010000143         5445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEPW010000143 DACEPW010000000
VERSION     DACEPW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.146"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(116..574)
                     /locus_tag="IW819_002146"
     CDS             complement(116..574)
                     /locus_tag="IW819_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1245241.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(587..1912)
                     /locus_tag="IW819_002147"
     CDS             complement(587..1912)
                     /locus_tag="IW819_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1245242.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1929..3341)
                     /locus_tag="IW819_002148"
     CDS             complement(1929..3341)
                     /locus_tag="IW819_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1245243.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3358..4122)
                     /locus_tag="IW819_002149"
     CDS             complement(3358..4122)
                     /locus_tag="IW819_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1245244.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4226..5101)
                     /locus_tag="IW819_002150"
     CDS             complement(4226..5101)
                     /locus_tag="IW819_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1245245.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5356..5440)
                     /locus_tag="IW819_002151"
     tRNA            complement(5356..5440)
                     /locus_tag="IW819_002151"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5404..5406),aa:Leu,seq:caa)
BASE COUNT         1664 a         1147 c          800 g         1834 t
ORIGIN      
        1 tcgataatta acaaaaatca aaatgattaa atctagtgat tgaaacatat catttcaaaa
       61 aaattttgta aattcaccca taaatatcac cctatttaac ttttttcatc gtacactaaa
      121 attcaaaaca actatcttta atctttactt cttggttttg ttttaataaa tttccttcaa
      181 aaatgcctgg agaaactaca atgatcaccg tatctttttg tgcttgctcc aacttcttca
      241 aatctataat agctaacagt gtcccttctt ttacctcctg gttactttca acccgtatct
      301 gaaacggact tccttcgagt tcaactgtat ctaaacccat atggatcagt atttttctgc
      361 cagattcact ttcgatcgtt atggcatgtc gggtaggaaa tatattatcg attattcctt
      421 taactggcgc atggatatat ccatcatgat tcgttatagc gtatccaact cctatcattt
      481 ttgaagaaaa gacctcgtct tttactgctg ttaatggaat aatttgacca ctagctacag
      541 cttttagaat gtttcttgtt ttaaagaaat gcacttttta tcacctctac tttaatgctg
      601 gccaatattt tttgtttact ttgatcattt catctagtat tttttttgcg actctggcat
      661 ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt
      721 aagcatctac tagtaacttc tctactgcaa cttgtgcctc catcaatcct ttatgaaaat
      781 cactgattgg ttttcttagt gtaattggct ccacaccagt tgcaccaaca tatgccggta
      841 tttcaacaac tgcatctgac cgtagattcg gaatcgcccc ttgattagga acgattagca
      901 taaagcgacg acgatcatca ttcaacaagg aaatagctat atcgacgata tattgtccat
      961 gctccccaaa attaaagttc aatacttctt ccacaatacc gcggcgtatt ttatcagcca
     1021 tttctttggt atttttctct cgtccatcca tgaccatatt agcacgtgta tactctttgt
     1081 cagtatgctc aacgaccata tcaggatata agtaatactc taagtaatta ttcgggatat
     1141 tacttggaaa atttttcgtg atgactgaca tcatttgaaa ggttttttgc caagttttat
     1201 ctcctatatt aaaatcggcg acttgcattt cttttgttat tagtttttca ataatctctg
     1261 gcataatttc tcgttgcaat tctttatcat aaatcgatgt ataccagcca aagtggttta
     1321 aaccataata cgtaacgatc caattcttac gatcataacc gtaattcact gcaatcgttt
     1381 cttcaattga aatagtcata tcacaggcat taatcatttt tacattcgga tatttacgac
     1441 gaacagcttc agaaacgatc gactctggat ttgtataatt caaaatccat gctttggggg
     1501 catagtcttg tatatgatcg acaatttcca ataaaccttt gattgagcgc atcccataag
     1561 caaatcctcc gagtccacac gtttcttgtc ctactaatcc atgtttaagt ggaatctttt
     1621 cgtccttttc tcgcatctcc aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc
     1681 ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg aatatttgaa aaaccttttc
     1741 ttttcagcat aaatttaaca atcaagtaca tatccatatt tctttcttca tcaatatcat
     1801 ataaacgtat ttcagataat ggcaaccgtt tttcattgtt caaaatggct tgaacgattc
     1861 ctggtgtata cgtactcccg cctccagcta tagtgataat ctgttttttc atcatcatct
     1921 tttcctttct attcttctaa taatttattg aattctgcca taacataagt cacttctaag
     1981 ccgatgataa tttgataatt tttttcagtt ggtttaatga tcccgcttat ccctaattgc
     2041 ttgattcgct gttcctcgac catggtggga tccactacct ctacccgtag gcgtgtatta
     2101 caactataga catccaggat attggctttt cctccaaacg cttgtagcat ttttgttgcc
     2161 atatattcat aatttttact tgctaaccct aactctgttt cttctaaggt tacttcattg
     2221 ccaaactctg ccttagcttc acgtcctaat gtctgaatgt ttctcttatt gatcaaataa
     2281 taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga ttggaagaat caaaatagca
     2341 ttggtagcga ttcgaaaatt gatgagataa tcaacaatac atgctccgaa tgtaaaacca
     2401 attcgtatat tgaataagta acagacaatc cctgctaaac cggcaaaaag tgcatggatc
     2461 acgtacaaca taggagaaag aaacatgaaa ctaaattcaa caggctcagt gatatttgca
     2521 ataaatgaag ttcctgcacc gcttcccatg attcctttga ccatctcttt attttcttta
     2581 taagctgctt tatagatagc tagacatata gctggtatac caaacatcat tactacaaaa
     2641 aaaactgata aaaacagtcc agcctgagga tctccgttga taaagcgtgt catctctcct
     2701 ctgaaaactt ccttgctagg agatgtatag cttcctaaat cgtaataaat atacgtattc
     2761 aacacatggt gcagtccaaa ggggatcagc aaacgattca aaaacataaa tatccccaca
     2821 cctaatgctc ccatatcaac gagcagtttt gcaaaattat caatcccaat ttggatgagc
     2881 ggccagataa agcccaaaaa aacagctgcg attgtttgta gaatcatcac catagtcaat
     2941 gggaattttt cgcccgcaaa ataagaaaaa actactggta atttttgttc tttaaagcga
     3001 ttatacgtcc atgccgctac taaaccggaa ataattccac caaaaatccc catattcact
     3061 tctgggtcca taatttctaa gccttttttc aaggtagcaa tcgctaaaaa accagtaagt
     3121 gcaggtattc ctttgtcctt atttttgaca aaaccgattg caactcctat agcaaataag
     3181 atatccaagt ttccaaaaac ggcatttcca gctgcaccaa ttactgggat gttcagcata
     3241 tcgtcagctg ataaacgcat gatcaatccc gctaatggca tcgctgcaac tggaagtagc
     3301 atcgctcttc caagcttctg cacatttgat ttaacattca tttttattcc tcctctatta
     3361 aatatcctca tatgttacta actcaatcaa aggatcattt ttcacttttt gagcaaatgc
     3421 tgaatgaatt aaaaattcga cttggtgggt acgaggatac acaaaacttg aaccatttaa
     3481 caattcctga tccaaatacg ctgcgtgaca catgacttcg atcgttttat cttctctcaa
     3541 tatcatgagt aaacttgcca aatattcttc cacactcgta ttgatttgtc gttttttttc
     3601 atcagtttct cctacgacat cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc
     3661 ttttcgttca aagttgctcc gaaccggcaa atcatatttt tttgctaatg taacaactac
     3721 ctcctgattt tcatccaaag tatgagtatg atgatgactg tctaaatgag ttggtatgat
     3781 ccccgcatga taaatttttt gaatctgagc attccattcc tgatacagtt gatctttgtc
     3841 aatgttaaat ggatgctgat aataagataa ggatctgaac tctccgcttt cgataagagt
     3901 atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt aaatggactc ctacacctag
     3961 cctaggcatt tcctttctta gtttcacagc atgctcgaaa cccggcatat ttgccatcaa
     4021 tgtagttgat gtcaaaattc ctcgctgata agcatccata atcccataat tcacaccatg
     4081 gctatagcca aaatcatccg aattaaaaat cacttttccc atttcattct ccttcttttt
     4141 tctactaaaa taggcgcaac caaataatca tagtccattt actatgtgtc atttggttgc
     4201 gcctatagat agttacgccc ttattttaat agattcttgc tagatgaatt gccaaataga
     4261 tgatttcatt ttcttgaatg gcaagttgaa attgctgttc gatgatatct ttaatcttta
     4321 gtgctgtaca atacgctttg ctatgtttaa atgtttcagt aaaaaaacca atattttcta
     4381 ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa tttgatatga accattaagc
     4441 gatgataagt cagtgattct ttatcataaa cgtttgaata ggatttttct agatgggaca
     4501 aaatcgtggt cgtcatttct actattttta agctctttaa actattaggc tctcctagct
     4561 cagcattaat gatatgcata gtgatgaatc ctacttcatt tgaccctaac tcggtttgat
     4621 atcgatgatt gattcgctcg accacttcta ctgctatatc atattcattt ggataaagga
     4681 atctcatttc tggcaacata attgctgtga catctctatc ttctcttttt cgttgaatag
     4741 caaaataaat atgatctgtc aatgtaatat agatatttga atctaatgga gaggagatcc
     4801 tagctgttat cagttcaatc acttcttccg caaaatccat aaattcgatt ggaatctgcg
     4861 agactatctc actgaatcgt ttgatctttt ctttagaatc taatacaaat aatttgtcag
     4921 catcctcaac atcaaatggc ttactcactt tagcagaaaa gccaatccct ttccccatcg
     4981 caattttttc tattccatcg tcatcgatca atacagcatt atgattcagc tttttcatca
     5041 catacaccgt taattcacct cctctatatg ataaaacaaa acaacttgca gactccttca
     5101 tacatatttt tgtctccaaa caatttttaa agacaaaaaa cgacctaaga aagaaccctt
     5161 tcttaggtcg tgcctaattg aatagtagca ccctacataa tttacaaaaa tagtttaaca
     5221 cataataaaa gcgttatcaa tatctttttt actcgtttta tgataatact tgtatcgaat
     5281 tgatttggag gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg
     5341 ctatattgat aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg
     5401 attttgagtc cagcgcgtct gccaattccg ccacgccggc aaatt
//
LOCUS       DACEPW010000144         5362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEPW010000144 DACEPW010000000
VERSION     DACEPW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.147"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..201
                     /locus_tag="IW819_002152"
     CDS             <1..201
                     /locus_tag="IW819_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAQ1245246.1"
                     /translation="IGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYIPEKVGVRIEDC
                     VYVTSDGCVPFTTTPKELLVLE"
     gene            complement(263..865)
                     /locus_tag="IW819_002153"
     CDS             complement(263..865)
                     /locus_tag="IW819_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1245247.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(868..1308)
                     /locus_tag="IW819_002154"
     CDS             complement(868..1308)
                     /locus_tag="IW819_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1245248.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(1434..2372)
                     /gene="galU"
                     /locus_tag="IW819_002155"
     CDS             complement(1434..2372)
                     /gene="galU"
                     /locus_tag="IW819_002155"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1245249.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(2387..3412)
                     /locus_tag="IW819_002156"
     CDS             complement(2387..3412)
                     /locus_tag="IW819_002156"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1245250.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(3456..4286)
                     /locus_tag="IW819_002157"
     CDS             complement(3456..4286)
                     /locus_tag="IW819_002157"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1245251.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(4301..5239)
                     /locus_tag="IW819_002158"
     CDS             complement(4301..5239)
                     /locus_tag="IW819_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1245252.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
BASE COUNT         1401 a         1204 c          948 g         1809 t
ORIGIN      
        1 attgggacaa ccgtccacga attcccttct cttgtagaag gaaacgactt agtgatcgaa
       61 gaagggatgt gcttctctct tgaaccagga atctatattc ctgaaaaagt aggggtacgt
      121 atcgaagatt gtgtgtatgt gacaagtgat ggttgtgtgc cattcacgac tacaccaaaa
      181 gaattacttg ttttagagta atgaaaataa aaaaggtggg agctggtccg ctctcacctt
      241 tttattctag aaatgagtat tcttagtgat tggtagtcga atctgtcatt gtatctttca
      301 tttctttttt agcttctttt gcatcttctt ttgccatttt caacatttct tttgtgtcat
      361 ctgcaactgg ttttgcttct gacattcctt cattgaccgt ctctcctgct ttatctgcag
      421 catcttttac atcgatcatg atatcttctg aagtatcttt agcaatacta cctaattcgt
      481 ctgctggttt tttcagatct tctgctgttt ttttgaaacg attagataaa tcagctgatt
      541 gttctttgag gtttaacaag atatcatctg attcacttcc tgcttcctct gctgattcac
      601 ttaccttttg cttaacatta cctgctagat cccctgcttg ttgagaaaga tcttgcgctt
      661 tttcttttgc aatgcctgtt aactcagctg atttttcttt tgccatatct gtgtaatctg
      721 aagccatgtc catcaaatca tctgcttgag cagccaattc atcccgaagt tctttaccag
      781 atttgggtgc taacaataat gcggcgattg ctgcggctgt acctccaact actgcaccta
      841 ataaaaatcc acctttttta gccattctta ttttcctcct gtcgtttcag tatgtttttt
      901 gtttttaaaa aatttcatcg ctgttccacc gacttttccg gcaacagtcg ctttagctgt
      961 cgtttttccg attccgccga ctttcgtcac gatgttttta ctagaatgat taagttccga
     1021 tacactttca cttaaatcag caactgctgt gaacaatgga tcgatcgttg cgacttttcc
     1081 attgatatcc aagagcagct cattactttt gaccagcaag ccttctactt gtctagataa
     1141 aagatctacg tcttttgtga cgatctcaat tgtcgtattt gcttctgcga cagtctggtc
     1201 tactttttct aaagttttgg aaacttgcaa caatacgcga atgatgaaaa cgacaagtat
     1261 cgcaaaggcg attgctgcga tcaatcctgc aatttctcct cctgacatga tcaaactccc
     1321 ttcttgtctc tcttttctat aaggatagca ttttcgtgtt ttttaaacaa ataaaaacga
     1381 gcttcaacta ctttttattc aattagcagc tgaagctcgt tagaaaaaaa tgattatttt
     1441 ttcttttctt ctttgatttc tgttattcct tttggattat gatcgatctt gcttccttct
     1501 gcttctaatg ttttacctaa agacatgatg tattcacgca aaggtgcccc tacttctgga
     1561 tgtgtcagac catattcaat acttgttttc ataaaaccaa atttgtcacc aacgtcataa
     1621 cgtttacctg tgaatttacg ggcaaatacg cgttgtgttt tattcaacgt atcgattgcg
     1681 tcagttagtt ggatctcatt tccagcacct ggttgttgtt ctgataatac atcaaagatt
     1741 tctggagtca ataagtaacg cccaataata gctaaatcac ttggtgcttt gcttggatct
     1801 ggtttttcaa caaagttttt gacattgtat aacccatctt ccagtacttc ccctgggttg
     1861 ataattccgt attttgaagt ttcatcgtga ggaacttgca taacggcaat cgtagaagcg
     1921 tgtgtacgtt catagtcatc gatcaattgc tgtgtcaatg gtacttcgtc ttccatgata
     1981 tcatcgccta gcataacgac aaacggttca tttccaataa aggctcttgc ttgaagaacg
     2041 gcgtgtccta acccctttgg atgagattga cggatgaaat gcaagttgac atctgtcgtt
     2101 tcttctacta atttcaataa ttcgattttt cctttttcac ttaggtttgt ttctaattcg
     2161 atatttgaat caaagtgatc ttcgattggt cgtttagctt ttcctgtaac gatcaagatg
     2221 tcttcgattc ctgatttcaa cgcttcttca acgatgaatt ggatcgttgg tttgtccacg
     2281 attggcaaca tttcctttgc catcgccttt gttgctggta aaaatcttgt tcctaatcct
     2341 gctgctggaa taactgcttt ttttactttc atatacattc gctccttcaa aaaattgtta
     2401 atgagaattc attttctttt ttcccatcac gtaacatgat ttcttttgcg gctgttttga
     2461 tttctttttg ttcatacagc acttcataga ttgtttgagt gatgggcatt tcgatttcca
     2521 tcatttgggc tagttccatc gctgcttgag tagtattcac accttcaacg accattccca
     2581 tattggctag cacctcttct agaggctgtc cttgtcctaa caagttgcct gcgcgccagt
     2641 ttctggaatg aacacttgtg caagtaacga ctagatcgcc tactccgctt aagccaataa
     2701 aggtcagcgg gttggcaccc attgctacac ctaaacggct gatctcagct aatccgcgcg
     2761 tcatgattgc cgcttttgca ttatcgccaa accctaagcc gtgaatagca ccagctccaa
     2821 tggcaataat atttttcaaa gctgccccca tctctacacc aaccacatca gggttagtat
     2881 agattcgaaa ataatggttc atgaacagct cttgtacata ttttgctgct tttgcatctg
     2941 tgcttgccgc tgtgatcgtc gtaatgtcat gctttgctac ttcttccgca tggcttggcc
     3001 cagaaagaac agcaacagct tttctttttt ctgtcgggat ttcctctaac aagatttcag
     3061 agattctttt atgcgttccc tgttctaatc ccttacttgc gtggatgatc actggcttcg
     3121 tattcaattt ggcaactagt tcttttgcaa cagaacgtat cgctttcgtc gggataacga
     3181 atagtaccgc atctgcatct tttactgcat cttccatatc ggtgaatgct cggatggaat
     3241 cgggtagatg atgatctggt aaataacgtt gattggtgtg ttgggtgttg atttcttcta
     3301 cttgtgaagc acgatgtccc caaatccgaa cgtcatgtcc gttttcagcg aggacttgtg
     3361 ctaaagcagt cccccaagag cctggtccta aaaccgctac tctataattc attttaccac
     3421 tcctgaaatt tttttcattt ttaacgctct aaaaatcaaa taatttgttg attttttcct
     3481 aatgagcggt cataatccgg taaatttcct ttttttctac gccaaataaa cagaatcaat
     3541 gtgccgacaa gtaaaacagc agacaataat tgagaaatgc ggatcacacc taaaaagtaa
     3601 aggctgtctg tgcgtaatcc ttcgataaag aaacgtccga aactatacca cattagataa
     3661 cataaaagga cttctccttt tttcaaaaga tgcggtttct tccgcaaaac caaaagcaga
     3721 ataaatccaa tcaaactcca aagtgattca taaagaaagg ttggttgtcg atagatccct
     3781 tcgatataca tgttatcaat gatgaattgc ggtaaatgca gactttctaa gaaacttcgt
     3841 gtcgtgtctg gaccatacgc ttcgtggttc atgaaatttc cccatcgtcc gattgcctgc
     3901 gccagtaata cacttggtgc agcgatatct aaaaaggtcc acaaagaaat aaaatgatat
     3961 ttggtaaaaa agtacagtgt gattgcacct gcaatcaatc ccccgtaaat cgctaatcct
     4021 cctgaacgga tattgaatat ttggattgga tcagctatat attggggaag atcgaataaa
     4081 acataataca aacgggcacc aataatggaa atcggcaatc cccacaacat aaaatctgtc
     4141 acgtcatctg cttttaatcc tacccgaaca gcttctctgc tgctgagcca catggcaata
     4201 atgattcctg atacgataat caatgcatac cagtagatag gaattcctac aagctcaaaa
     4261 gcaactcgat tgatttgtgc taacatattg tcactccttg ctagtcattg cctgagtttt
     4321 cttcaatcaa gcgacttaat ctttcttcaa atgttttcgt tgcgtcaaag cccattgtac
     4381 gcgcgcggaa attcatcgcg gctacttcaa tgatgattgc aacgtttcgt cctgttttga
     4441 cagggatttt tatttgtgga atgcccactt ccgcgatttc aacggtcgtg tcttcagaac
     4501 ccaaacggtc gtatttctta tctttcgccc atgcttctag ataaacagct aactggactt
     4561 gcatagagcc tcgtaccgca ctcgcaccga aaagattcat tacatctatg atcccgatcc
     4621 ctcgaatctc aatcagatgt tccaggattt taggaggttc tccgattacc gtcagttcat
     4681 cttgctgata gacatctact cgatcgtctg cgatcaatcg atgtccgcgc ttaatcagtt
     4741 ccaacgccgt ttcacttttc cctatcccac tgtccccttg aattagtaca cctaatccgt
     4801 agacatcgac taacacaccg tggacacttg ttcgaggggc taagcgccca tctaaataac
     4861 tagataattc acctagcagc cgagacgttg agataggcga acgcagcaca gctatcccat
     4921 tttcattggc tgcttggatc atttcttccg gagccggcaa tcctcttgaa ataataaatg
     4981 cgggcatatc ttcactacat aatcttctca tgatgatcaa acgctcttct gggatcatcc
     5041 gttccgcaaa actgatttct ttactgccaa atagctgtaa acgattatga gaataataat
     5101 taaaataacc ggtcaactct agtcctgggc gtgagatgtc cccagtcacg atgacgcggt
     5161 ctaaactttt ctcgtcgcct gtgacgattt ccaaagataa tttttttact aattggctaa
     5221 cttttacaac ttctgacatt catgctacct catttctatt agagtgttct ctaccatttt
     5281 atcattgtct agccaaaagt actaccaatt aaaaaaaacg tttttattta attaccaatg
     5341 taatagaaaa cgcattctta at
//
LOCUS       DACEPW010000145         5337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEPW010000145 DACEPW010000000
VERSION     DACEPW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.148"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..224)
                     /locus_tag="IW819_002159"
                     /pseudo
     CDS             complement(1..224)
                     /locus_tag="IW819_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(383..1084)
                     /locus_tag="IW819_002160"
     CDS             complement(383..1084)
                     /locus_tag="IW819_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1245253.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(1192..2634)
                     /locus_tag="IW819_002161"
     CDS             complement(1192..2634)
                     /locus_tag="IW819_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1245254.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(2648..4513)
                     /locus_tag="IW819_002162"
                     /pseudo
     CDS             complement(2648..4513)
                     /locus_tag="IW819_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(4634..>5337)
                     /locus_tag="IW819_002163"
     CDS             complement(4634..>5337)
                     /locus_tag="IW819_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1245255.1"
                     /translation="RDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIY
                     LTLTDHLHYAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDE
                     AGFIAFHFVNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQ
                     FFAQRYLKGEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLT
                     IHIQRVVSEKKSIQS"
BASE COUNT         1532 a         1204 c          920 g         1681 t
ORIGIN      
        1 gctatgacat tgcgtgccaa gtcgttcaat acgataggga aacatccctc tgtgatctga
       61 cagatcccca taggaaaagc ggtcttcaat gtatcgattt cttgacgatt gaatatattt
      121 ttacgaatga ttttccctaa gaaataagcg atcgtcaagc cgaatggggt taccatcgaa
      181 gctaaaatca atacagtgat tggttcatag attccttctg aaaatcgtgc cacaccccct
      241 tatccagaat aaatggtaat ccagaaacag ccgtgtatct gcggtaataa gcctaaccaa
      301 acgaaaaata tgagaagcta tttttgtttg gctgttctta ttacttgatg cagggcagct
      361 ttttcacaac ctcttttttt atttatgttt cataatcaat atggtaagga tgcagctccg
      421 tatggtagac cgcctccgaa tattcaatca tttccccaat ttgcgtagac gatttccgta
      481 tccgtttgat gccgatctgt gcgtctgttt tcagaattcc ttgttcttct tctgttaaaa
      541 atactgccga gaaatcatca gaaaatgagt caatagtata tccttttttc gtcagcaaac
      601 gatacaaaga ttctttgcca aattcttctc tgctgaattg catcatttct tgtggaagat
      661 aatgaacaaa ataaatataa ggctgattgt tcaaataata taagcgctta aaacaaacag
      721 cttgctcacc gaaaaaggaa aacaaaggat cttccttctt tatatggacc aattgcaaat
      781 ccagcgtttc tttccggact tcatatcggg agttctccaa gatttgcgta aagcttgccg
      841 ctttggataa tcgattgtaa ggacgattgc ttagaaccgt cgtcccacga ccacttttct
      901 ttgcgacata ttcatctgat gccagcagtt cgattgcctt tctgacagtg attttgctga
      961 cttgaaacag ttcttctaat tcactttctg tcggtaacat cgaaccgact ggatattttc
     1021 ctgagaaaat atcttcttta attttatctg cgacttccaa gtatagtaca cgtgtccttt
     1081 tcataacgtc accctttgct ttactattac taaaagaata gcacataaaa aataaaaaaa
     1141 gaacacaaat cacatgtgag aagtgtgttc tttttcattc agctgcttta attacaagtt
     1201 tacgccattt gaggcaatta cttctttata ccaatcaaaa gattttttct ttgatcgatt
     1261 cagtgtccca tttccttcat cgtctagatc cacatagata aagccgtaac gtttactcat
     1321 ttctcctgtt gatgcactga caagatccat acatccccaa ggcgtatagc ccatcaaatc
     1381 tacaccgtct gctactgcat tagccatcgc ttcgatatga cgacgtaaat aatcgatccg
     1441 ataatcatca ataatcgtgc catcctcttc catatgatca atggctccaa gaccgttttc
     1501 aacaatgaat aatggtttct ggtaacgatt gtataattca ttcaatgcga ttctcagccc
     1561 ttctggatcg atctgccagc cccattcact tgcctcaaga aacggatttt ttactcctcc
     1621 cagcaagttc ccttctgaag ttgctgcttg tggatcagtc tcattgattg ccgtagacat
     1681 ataataacta aatccgatgt agtccactgg atattctttt agcaactgga agtctttttc
     1741 cgtttgctct aatttttccg gatcgactcc atatttttta tgggtacgcg ctgtgtatgc
     1801 agggtattct ccacgtactt ggacatccgt gcagaaaaaa ttgaattctt ggttttggat
     1861 cattgttgct gcctgattga ctggattggc atcgatgctg taagttgttg catagataat
     1921 catgcagccg atttggatat ccgaacgcaa ttcgtggccg atttttacag ctttgctact
     1981 tgcaacgaat tgattatgcc atgcttggaa aatattttgg tactctcctg ctccattgct
     2041 ttttactaat ccttgactta aagctggaaa gtgaaaggcc gaattgattt cattgaaagt
     2101 catccaatat ttgactttac tgctgtatct ctctaatact gtctgagcaa atcgttcata
     2161 gaagtcgatc agttttcgat ttttccagcc accgtattct tttgccaaat gaagaggcat
     2221 ttcgtaatga gaaatcgtga tcacaggttc aatatcatat tttaagcact catcaatcag
     2281 ctgatcataa aattctaatc ccgcttcgtt aggagtacct tcgtctccat ttggaaaaat
     2341 tcgtgtccag gcaatcgaaa aacgataaca tttgaatccc atttccgcaa acaaggcaag
     2401 atcttctttg aaacgatcgt aatgatcgat tcctcgatga ttcgggtaaa tatatttttc
     2461 ttgatcaatc gtccagtcaa attcttcact gccgataatc gcaaagcgct gcttacctcc
     2521 aggcatagca tctgcaacgg atagcccttt tccgtctcga tcataagccc cttctaattg
     2581 attggcagca gttgctccac cccataaaaa tttttctgaa aaacgtgatt gcatcctgat
     2641 ttcctcctta ttttacaatt gccagaacat cttcgtttgc aataatctct ttttggtcta
     2701 attcaagtat atcggcaaat gcatcagtat tcgtaatgac aactggagtg attacgctat
     2761 agccagctgc tctaattgct tcgatgtcaa aacgaatcaa taaatctcct gcttggacga
     2821 gatcccctta tttgactaag atctcaaagc cattcccatt taattctact gtatccatcc
     2881 cgatatgaat caaaatatcc gctccgtcag tcgttgtgat ccctacagca tgtccagttg
     2941 gaaaaatagt cgtgatctct ccgtctgcag gtgcaactag ttctcctact tctggatcaa
     3001 tagcgatacc ttttcccatc acgccagaag aaaaaacttg gtctggtact tcactcaatg
     3061 gcagtacttt acccgttaaa ggacttttta aggtatgccg tgctgaagta atcggttctc
     3121 ctgcattcgc atgtttgttt tctaattgat tgtcttgtgt ttgttcgtca aatcctaaaa
     3181 tcaatgtacc tacgaatgca agaacaaaag caattcctgt agctattact gctactgtca
     3241 catttgactc tacaccatcc accgtactaa tgaaagtcgg aatcgtcaat aagctgacca
     3301 aactgctgga gaaagctttt actccggaaa atccgataat agctccacca atacctccag
     3361 aaatacaagc ggcaatgaat ggtttcttca aaggtaatgt cacaccatac acagttggtt
     3421 cagtgattcc aaataacgaa gtcactgtag aagaaatccc taatgctcga agtttcgtat
     3481 cttttgttcg caatgctaca gctaaagcag ccccaccttg tgccaagatg gctggaagaa
     3541 gcattggcat caatacatca aacccatatt gttcgatatt caaaaacatg atcggtacga
     3601 atccccaatg catgccgaac atcacgaata cttgccataa acttcccatg attagacctg
     3661 ctacgattgg actaaatcca taaatcgagt tgaagagacc gcctaaagca ttaccggcaa
     3721 ccgtcccgat tggtcctaaa gcaagaaatg ttaatggaac catcactact aaaacgacaa
     3781 gcggaactaa aatcatttgc agaaattgcg gaatcacttt ttttacgaat ggctctaatt
     3841 tactttgaac ccatacggct agaatgattg gtatgacaga agaagtataa ccagatggtg
     3901 aaagaatcac tgggatccca aagaaactga tagcttctcc cgcaccagca actgctgtga
     3961 tagctggata cacaagtgcc atagcaattg ctactgctaa aaactggttg gctttgaact
     4021 ttttagctgc tgtaaaagca agcatgacag gtaagaaggt aaagacacca tctgctgcag
     4081 caaacaatat ttgataagta ccgctatcag ctgtcagcca gcccatcacc gttgccaatg
     4141 ataagaatcc tttcaatacc ccggcggctg ccatcgcccc tagaaaaggt gtaaatatac
     4201 tagagataat atcgatcaga cgattcaaaa tatttttgga ctcttctttt ccatcctccg
     4261 aaactgcaag accttcctca tccacgatcg attgataaac atctgctaca ttactgccaa
     4321 tcactacttg atactgaccg ccactttgta cgacttggat cacgtccggg tcttctttta
     4381 acttttgcgt atctgctaaa gattcatctt ttaatctgaa tcgcaaccgc gtagcacaat
     4441 gtactaaact tttgacattc ttttgtcctc ctaccgcttc ccatacacga cgaccaactg
     4501 cttgattatc cattgttttt tccttctttc ttttttattt aaaaaataaa aacccagatt
     4561 gaaaaaaaca gtcacaaaca ttcgtgaacc tattttttca atccaggttt tgcctgccat
     4621 acagtaacaa tccttatgat tggatgcttt ttttctcact tactactctt tgaatatgaa
     4681 tcgttaaata aatctgctct gcgatcccca tttcctgttt ttgcgtttgt cgaagatact
     4741 cattgatccg ttcgacacaa tgaaacgctt ttggatactt tccttgaact aatgcgtaga
     4801 gaaattcatc ttgctcgtca gatccttccc ctttgagata acgctgggca aaaaactgca
     4861 gatgagtaat gatacgctga taattcaaag attcttcatc gaacacgatg ccgaagaatt
     4921 tactgatgat actcagtata tctcttacca ttgtggtagc ggtcatcgtc acttgcatgt
     4981 cttcgtttcc ttgttggcta ttcacaaaat gaaaggcaat aaaaccggct tcatcctcag
     5041 aaagttccac acccgtcttt tgtttcacga acaaaagagc ctctttcgct gcttgatatt
     5101 ctttcggata aaattttttt gtttcaaata ataatggatt aggcagttgg atattttctt
     5161 ttgctcgtgt gatcgcataa tgaagatgat cggtcaatgt cagataaata ttaggcgaaa
     5221 attcaacttt tagtttatct tcagccagtt cgactacttg tttgacccat tcgatctcct
     5281 ctgccggaat atcagccatc agctgctgga attggttttt agcaaaagaa tctctta
//
LOCUS       DACEPW010000146         5335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEPW010000146 DACEPW010000000
VERSION     DACEPW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.149"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(186..731)
                     /locus_tag="IW819_002164"
     CDS             complement(186..731)
                     /locus_tag="IW819_002164"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1245256.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(735..863)
                     /locus_tag="IW819_002165"
     CDS             complement(735..863)
                     /locus_tag="IW819_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1245257.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            complement(856..1947)
                     /gene="phnW"
                     /locus_tag="IW819_002166"
     CDS             complement(856..1947)
                     /gene="phnW"
                     /locus_tag="IW819_002166"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1245258.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            complement(2164..2643)
                     /locus_tag="IW819_002167"
     CDS             complement(2164..2643)
                     /locus_tag="IW819_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1245259.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            3002..3784
                     /gene="rpsB"
                     /locus_tag="IW819_002168"
     CDS             3002..3784
                     /gene="rpsB"
                     /locus_tag="IW819_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1245260.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3883..4764
                     /locus_tag="IW819_002169"
     CDS             3883..4764
                     /locus_tag="IW819_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1245261.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            4900..>5335
                     /locus_tag="IW819_002170"
     CDS             4900..>5335
                     /locus_tag="IW819_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HAQ1245262.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTT"
BASE COUNT         1729 a         1003 c         1077 g         1526 t
ORIGIN      
        1 gaggagctat ttttgcttgg ctgtttttat tacttgatac aggacagctt tttcacaacc
       61 tcttattttg tataaaagtc attctggatt cttgtcagcg tacaaaatgt attctgttaa
      121 ctcttttaaa ttgtttacac aaatatcagc gccggcttgt tggaatctaa gagcaacaga
      181 ctgttttaaa cgatcttttt ccccatctct aatttttggt actcatcata agaaatcccc
      241 attagcgaac taccttcaat tacacctact gtcaacactc cggcattttt cccttcctgg
      301 atatctgaga ccgtatctcc gactttgatt acttcatcaa ccgacttgtt tttgaagtac
      361 tgcatgtttt tatggatcat ataaggaaac ggtcgaccat acccttcgac gtcttcaaaa
      421 gttgccatga agtctggtga atacctttct ttttttgcgg tttctgctac gattgtcatc
      481 ataggagcat taaatccatt cgtggaacca atattgatcc cagcgtttct caaccgttcg
      541 atcgtttcca atgtgtcggg tttcaaggta gaatgcttga ccaaatcttt aaacagataa
      601 tgttcaaatt gttgatacaa ttcctccacc tctttttcct gaggtagttg tccataaata
      661 gctatccatt gttttttgac agaagggagg gaaagaagct gttggatatg atcccatttt
      721 aattttccca tcggttagcg gatttctttg tcagtcagtt gggttccttt ttctagaaaa
      781 gcatttctaa aagcgtggac aggagccata cagccaaagt ccacagttgt tcctgcccaa
      841 tcaaaaatta cagttttaat cattaacgtt ctccttatag tctttgataa ttgctgtcag
      901 ttccaaaata tcttccggat agatctcgcc aatatttccg atacgaaaac attcatgatc
      961 gctgattttc ccgggataga tcacaaatcc attttgcttt aaatactgat aaaaatgatt
     1021 aaaatcaaat cgccagtcct tagggtaaag aaaagaggtg atgaatggcc cctgatattc
     1081 ttgaatgaaa ggctggaacc ccatctctgt cattttttgt cgtagcacat gattattggc
     1141 gagataacgt gaatatcgag tttcgattcc tccttctgcc tctagttcct tcagcgcttg
     1201 ttgaaaagcc aaaaccgtat gggtagggga agtgaaacgc catttgccat tctcgttttc
     1261 catcacgcgg tactggtcat atagatcaag tgacaaactt cggctaacat tttttcgttt
     1321 ttccagttcc gctttattag caagtacaaa agaaaagcct ggaacacctt gaatacattt
     1381 gtttgagctg gaaatcaaaa aatcaatccc tagttcggct acacaaatcg gtatcccacc
     1441 aaagcttgac atcgcgtcca ctataaacaa aagatctctt tcttgtgtga cagcagcaat
     1501 ctcttccaaa ttattcaaaa taccggaagt agtctcgcta tggaccattg ctagtgcgga
     1561 gatagacgga ttctcttcaa tcagctgtgt aatcacatga gcttggagga tttgatgttc
     1621 cgatacctca taaacggtat agttcagttt cagacattcg cacatgttga tcattcgttg
     1681 tctatatgcg ccgtttgaac aaatcagtac agtatcttct ggcctgatca cagaacttaa
     1741 taccgattct accccgaagg agctgctacc ttgcattaag accgttgtgt atattccttc
     1801 cgatacttga gcaagatcca ataatttttt tcttatccat tgtgtaattt ctttgtattc
     1861 ttgatcccaa gtacaatgat cgatcagcat agcatttttc actgtttcag aagtcgtcaa
     1921 tggtccagaa gttaataatt tatacatatg atttcctttc atttcttttt tgaaatacac
     1981 aaagagcaag aagctgctct tgttaacatc caatataaat gatgattatt gtgattcaat
     2041 caaggtgttg aaaaatttga ataaaaaatg atgtattttt gtaaagagtg taaaggttta
     2101 ttcaataagg aaaatattga aaataaaaaa acacgcattc catattagga atacgtgctc
     2161 tgattaaaaa agtgatactt cagttggatg gtgaagaaat tcttctactc gttttgcgtc
     2221 ctcctcattt tccgagatga ccatgatcgt atcgaatcca gcaattgtac tgacaataga
     2281 gggaggtttg atctcatcta aaacagatgc aaataaatga gcattgtctg gcaaggtatt
     2341 gacgatcgtc aaaaagtgga ctcgttcgac tttcaataca atgtcttcaa tcatttgaac
     2401 cagccgatcc tcttcctcag tcctagtctc aggagttcct ttttcgcgaa acaatacgaa
     2461 gcgggaaacg ccattttcat caggtgcctt cacgattttt agatcacgga tatctcgaga
     2521 aatcgttgcc tgcgtggcgg taacgccata atgcccaagt ttgtttaata gttcttcttg
     2581 cgtacggatc gggaattctt caatgacttg tttaatcagc aaatgtcttt gagctttgcg
     2641 cattttcttt ctcctttacg aaagatttca catgtatcat aacaaaaaaa agaaggattg
     2701 cccaataata ttcgtctttt atacctaaaa ttattttatg gaaaaaggat aataaagaaa
     2761 caaagagaaa tctactgttt ttcttgaatt ctttttatct ttcaggtaaa ctattgaatg
     2821 ttgagcagaa aagtatattt gctcttgcga tacggatctt ttgtctgtat aatagctgag
     2881 ggtgctttat gcatcagcaa atccacattc ttattggatt gtcggtaggt gcttgaaaaa
     2941 gtttgccggc aaggtagaag ataagaagag gaaaaataaa accaaatcgg aggaaacaaa
     3001 catggcagta atttctatga aacaattact agaagccggc gtacactttg gtcaccaaac
     3061 tcgtcgctgg aatccaaaaa tgaagaaata tatcttcaca gaaagaaacg gaatctacat
     3121 cattgacttg caaaaaacag ttaaattagt agatgcagct tatgattaca tgaagagcgt
     3181 tgctgaagaa ggcggcgtag cattattcgt aggtacaaaa aaacaagcac aagaagcaat
     3241 caaagaagaa gcaactcgtg caggacaata cttcgttaac catcgttggt taggcggaac
     3301 attgacaaac tgggatacta tccaaaaacg tatcagccgt ttgaaacaaa tcaatgcaat
     3361 ggaagaagat ggaacttttg aagttctacc taaaaaagaa gttgctggtt tgaacaaaga
     3421 acgtgaacgt cttgaaaaat tcttaggcgg tatcgctgat atgccaagaa tcccagatgt
     3481 aatgtacatc gttgacccac gtaaagaacg tattgctgtt caagaagcac acaaattaaa
     3541 tatcccaatc gtagctatgg ttgatacaaa ctgcgatcca gatgaaatcg acgtagtaat
     3601 cccttcaaac gacgatgcaa ttcgtgccgt taaattgatt acttcaaaaa tggctgatgc
     3661 tttcatcgaa ggaaaccaag gtgaagatca agtagttgaa gaagatttca cagaagaaaa
     3721 cacagcaaca tcaatcgaag aaatcgtaga tgttgttgaa ggcgacaatt cttcagcaga
     3781 ataattttga atgatggagc tgtttcaaag gctgggcgac aagcctcact ctcctttgaa
     3841 gcagcttttt ttaaaaagaa aaacacacag gaggagttaa acatggcaga cgttacagct
     3901 aaaatggtaa aagaactacg cgaaatgact ggcgtaggaa tgatggatgc gaaaaaagca
     3961 ttagttgaag tagaaggcga catggaaaaa gcagttgatc ttttacgtga aaaaggaatg
     4021 gcaaaggctg cgaagaaaaa tgaccgcatt gcagctgaag gattggcttc agttgctgtg
     4081 aaaggtaaca ctgcagcaat cgttgaagta aactcagaaa cagactttgt ttctaaaaac
     4141 gaaatgttcc aagatttagt aaaagaaatt gcagaacttg ttgctgaaaa caaaccagca
     4201 gacatggaag ctgcaatgaa aatcaaaaca gataaaggta caattgaatc tgacttgatc
     4261 gaagcaactc aagttatcgg agaaaaaatc agcttccgtc gctttgaagt tgtagaaaaa
     4321 gacgacaatg ctgcatttgg tggatacttg catatgggcg gacgtattgc cgtattgact
     4381 gttctagatg gaacaactga cgaaacagtt gcaagagacg tagctatgca cgtagcagct
     4441 atcaatcctc gttatgtaaa cgaatcacaa attccagaag ctgaattaga acatgaaaaa
     4501 actgttctta cagaacaagc attgaacgaa ggcaaaccag cgaatatcgt tgaaaaaatg
     4561 gtagaaggac gcttgaagaa attcaaagca gaaatcgctt tggtggacca accattcgtt
     4621 aaagatcctg atatgactgt tgaaaaatac gttgcatcaa aaggcgcaac tgttaaaaca
     4681 ttcgtacgtt tcgaagttgg cgaaggtatc gaaaaacgcg aagataactt tgtagaagaa
     4741 gttatgagcc aagtgaaaaa ataattctgt tgaataacag acgggaacgc acggtttgtg
     4801 cgctcccttt ttttaagcaa aacaagaaaa accagtctta gagtgtcgaa atcatctaag
     4861 tatggtaaaa tggcaataga aaatattgga ggaatgaaca tggtcacacc gaaatatcag
     4921 cgtgtagtct taaaattgag tggagaagct ttagctgggg acgcaggatt tggaatcaaa
     4981 ccaccagtaa tcaaagaaat catccaagaa atcaaagaag ttcacgagct gggcgttgaa
     5041 atggcaatcg ttgtgggcgg cggaaatatc tggcgcggac aaatcggagc acaaatgggc
     5101 atggaacgtg ctcaagctga ttacatgggt atgttagcta cagttatgaa tgccttagct
     5161 ttacaggata cgttggaaaa cgtgggtgta ccgacacgcg tgcaaacttc gattgaaatg
     5221 cgtcaaatcg cagagccata tattcgccgt aaagcagaac gtcatttaga aaaaggacgg
     5281 atcgttatct ttgcaggcgg taccggcaac ccgtacttct cgacagacac aacag
//
LOCUS       DACEPW010000147         5302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEPW010000147 DACEPW010000000
VERSION     DACEPW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.150"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            376..1596
                     /locus_tag="IW819_002171"
     CDS             376..1596
                     /locus_tag="IW819_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1245263.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1593..4313
                     /locus_tag="IW819_002172"
     CDS             1593..4313
                     /locus_tag="IW819_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1245264.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4297..5241
                     /locus_tag="IW819_002173"
     CDS             4297..5241
                     /locus_tag="IW819_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1245265.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         1979 a          861 c         1051 g         1411 t
ORIGIN      
        1 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
       61 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
      121 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
      181 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
      241 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
      301 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
      361 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
      421 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
      481 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
      541 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
      601 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
      661 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
      721 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
      781 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
      841 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
      901 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
      961 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     1021 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     1081 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     1141 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     1201 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     1261 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     1321 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     1381 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     1441 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     1501 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     1561 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     1621 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     1681 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     1741 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     1801 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     1861 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     1921 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     1981 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     2041 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     2101 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     2161 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     2221 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     2281 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     2341 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     2401 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     2461 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     2521 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     2581 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     2641 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     2701 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     2761 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     2821 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     2881 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     2941 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     3001 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     3061 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     3121 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     3181 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     3241 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     3301 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     3361 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     3421 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     3481 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     3541 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     3601 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     3661 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     3721 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     3781 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     3841 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     3901 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     3961 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     4021 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     4081 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     4141 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     4201 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     4261 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     4321 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     4381 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     4441 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     4501 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     4561 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     4621 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     4681 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     4741 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     4801 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     4861 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     4921 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     4981 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     5041 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     5101 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     5161 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     5221 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     5281 acataagtct tcgctttttt tg
//
LOCUS       DACEPW010000148         5281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEPW010000148 DACEPW010000000
VERSION     DACEPW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.151"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2280
                     /locus_tag="IW819_002174"
     CDS             <1..2280
                     /locus_tag="IW819_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1245266.1"
                     /translation="FSPDSQAIGAYYAKETVTLLEREGQVYRPSFILGIKVKEKATVE
                     SFYDVLNLIWLDVAGSLARLFGLEVDDLETLFSESDPYVKEVTQLLYSFSIRPLVKKE
                     LLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTYVRMPKEHKGLLQLEDELGTSYLALL
                     PIGETPENMRNCDLFQFAQDLSFPAELRMKIHYPRLKGQGGIGLKLNWLSERFKTEYK
                     ELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPVVDWIACFIVYGQTVEEVRQNSATII
                     QQLHTTIHIHRAKNDQRELFYKCLLGQPLGRQLHWKHRTTVEAIGEWLFSAVSELGME
                     SGWYIGRQDTLGDGGSRGEKTPLEELIYASNRFVFLNLLAIAEGIKGALFDAPHVAVT
                     GKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGEKRKWFTKLIQDPYYQKHYPLFVEHL
                     QKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQVAQDMIDELSPLGTNHLLKGAVLKGI
                     QEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREYGEFLRLTVEDSILRLGFSYGENPGL
                     DFNAKTTILEIQDLKLPNDNVRPELYTDADRKSLCLMISLGRFCEMFGKRDSSKKTAI
                     YFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLVLDTQFIGDLGSDKEKGNFGVVFAFD
                     EDSEREKILTHLRLEVNEANLSEMSHMIKGQCWMLDPYGRVGKLNVHSLFEEMTAALQ
                     TTEKTSHSQIEEQDTLIMQ"
     gene            2296..4506
                     /locus_tag="IW819_002175"
     CDS             2296..4506
                     /locus_tag="IW819_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245267.1"
                     /translation="MAYIKRKEKKWCVWLFLTVGFGLFFVGGLVYAVEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPAFDYMIMNTLKSFLSLMFQKSFYSVIILVAFLIY
                     NIVDDLILMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGGQVINPFELGGRSNQDLPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIHQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQRQIQRSSESKPSISVP
                     KSLQPAFSSFNNPVEQSKKIEKKNETLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            4514..>5281
                     /locus_tag="IW819_002176"
     CDS             4514..>5281
                     /locus_tag="IW819_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAQ1245268.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNWE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESTYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKDCYDYYGFLVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQ"
BASE COUNT         1680 a          829 c         1167 g         1605 t
ORIGIN      
        1 ttttctcccg atagtcaagc aattggtgcg tattatgcaa aagaaacagt gacattgttg
       61 gaacgagaag gtcaagtcta tcgtccttcc tttattttag gaatcaaagt caaagaaaaa
      121 gcaaccgtgg agagttttta tgatgtacta aatctaatct ggttagatgt agcaggaagt
      181 ttagcacgct tatttggatt agaagtagat gatttagaaa ctttgttttc tgaaagtgat
      241 ccttatgtaa aagaagtgac acaattactt tatagctttt ctattcgtcc cttagtaaaa
      301 aaagagctgt tgtatctcaa tcgtctgcaa tatattcgag gaatggctca taaagtagaa
      361 gaacaaaata attgggaagt actgaaagac aatcaaggaa cgtatgtacg tatgccgaag
      421 gaacataaag gccttcttca attagaagat gagttgggga ctagttattt agcgttgtta
      481 cctattggag aaacacctga aaatatgaga aactgtgatc tatttcaatt tgcacaggat
      541 ctttctttcc cagcagaatt acgaatgaaa attcactatc cacgcttgaa aggacaggga
      601 ggtattggtt taaaactaaa ttggctatcg gaacggttca aaacagaata taaggaattg
      661 gttcaaaacg atgaagaacc tagcgaacgc ttattaactg ttcggggatt agtaaaacat
      721 ttaaagcaac gagtagataa tggccaacct gtcgtcgatt ggatcgcatg ttttattgta
      781 tacggtcaaa cggttgaaga agttcgtcaa aatagtgcaa cgattattca acagcttcat
      841 acgactatcc atatccaccg tgcaaaaaat gatcaacgag aattgttcta taaatgttta
      901 ttagggcaac cattgggtcg acagttgcat tggaagcacc gaacaactgt agaagcaatt
      961 ggcgaatggt tgttttcagc agtatcagaa ttaggtatgg aatccggctg gtatattggt
     1021 cggcaagata cattagggga tggtggttct cgaggagaaa aaacaccatt agaagaatta
     1081 atttatgcgt caaatcgttt tgtctttttg aatcttctag ctattgcgga aggaatcaaa
     1141 ggtgcgttgt ttgatgcgcc tcatgtagca gtaactggaa aaaccggtaa agggaagacc
     1201 tttttagttg gattgctctt tttgtatagt tcgtttatga atgtacaaag tttatttgtg
     1261 gatcccaaag gagaaaagcg caaatggttt acgaaactca ttcaagaccc gtattatcag
     1321 aaacattacc ctttgtttgt ggaacatttg caaaaaatga gttatgtcac gttagatgct
     1381 acaaatcccc gtaattatgg cgtattagat ccaattgttt gtttatcccg agtagatgcg
     1441 aaacaagtag cccaggatat gattgatgag ttaagtccgt taggtaccaa tcacctttta
     1501 aaaggggcgg tattaaaggg aatccaagaa gttttagttc aaaaagaagc cggacaacaa
     1561 gttggcttga tgcatgtggt cgagtattta gaacaaatgg aacaaaaaga ggttcgagaa
     1621 tatggtgagt ttttacgctt gacggtagaa gattcgattt tacgtttagg attttcttat
     1681 ggagaaaacc cgggattaga ctttaatgcc aaaacaacga ttttagagat ccaagatttg
     1741 aaattaccaa atgacaatgt ccgaccagaa ctatatacag atgcggatcg aaagtctttg
     1801 tgtttgatga ttagtttagg tcgtttttgt gaaatgtttg gaaaacgaga tagtagtaag
     1861 aaaacagcga tttattttac agaagcatgg gtatttaata attcgaacgc gggacgtagt
     1921 attattgcag caatggcgcg tgtggggcgt tcacagatga atcaattagt gctagataca
     1981 caatttattg gcgacttagg ttcagataaa gaaaaaggga attttggtgt tgtcttcgct
     2041 tttgatgaag acagtgagcg agaaaagatt ttgactcatt tacgtttgga agtaaatgaa
     2101 gcgaacttat ctgaaatgag ccatatgatt aaaggacagt gttggatgtt agatccctat
     2161 ggacgtgtag gaaaactcaa tgtacacagt ttgtttgaag aaatgaccgc tgcattacaa
     2221 acaacggaaa aaacaagtca ctctcaaata gaagaacagg atacgttaat catgcaatag
     2281 aaacgaggtg aaacaatggc ttatataaag cgtaaggaaa agaaatggtg cgtttggctt
     2341 tttttaacgg ttggattcgg actgtttttt gttggtggat tggtatatgc ggtagaaaaa
     2401 caggtggatc aatcgatcta tgagatcggg aaatttcata ttgatacctt ttcttcttat
     2461 ttatctgata gtgaattttc attaggcaaa atgttatttg gagcaaatgg cacagatttt
     2521 ctaaaaatga tgacgaatat gttctttagt ttaagtaaat tgatttggca aggccttgat
     2581 tttgtcattg agaagctcta tgaaggagct gcaatgaacg agttaattca ccagtttttc
     2641 actttttcgg atactattta ttcgaaaata tacgatccta ttggtgcggg gattgtcgtc
     2701 ttgtatgtgt tatatctatt tactcttcgt atatttagag ggaaacatta cgcaaaaatg
     2761 gtgtttattc gatttctctt tgttgtcatt ttttcaatgg tttggtttgg ctatggtagt
     2821 aacgtaccta gtaaaggaga agcttttact aaagggttaa atgatttgtc cgtggaggta
     2881 gaaggcttta ttttttctgc tacaaatagt ctagatgaat tggcgacaaa agatgtcaat
     2941 ggtgttttag tgccaacgac acaaaaggaa gccattcaaa aagtgcgcca actttattat
     3001 aaagcggctg ttgttgatcc gtatctattg ttaaattatg ggacaacaaa tttagaagaa
     3061 ttgtatcaag cagagattga accacaagaa tttctagcca aggaagcgtc aagtgaagaa
     3121 ttatctgata ttgagacaaa agtgaccact gctgcaggag aagatagagg gaaaaagaag
     3181 gcttatcgtg tctatattca accaactaaa gctgtttata aagcagtcgt tggttctatg
     3241 gtgccattgc ttaatctaag tattggatta ccacttgtag tgattggcat gattcgtttt
     3301 atctttcaaa tgttggtgtt gtgtttatta cttgtgattc ctttttcatt actcgcttct
     3361 tttgttccag catttgatta tatgatcatg aataccttaa agtccttttt gagcttgatg
     3421 tttcaaaaat ctttttacag tgtgattatt ttggtggcat ttttgatcta taacatagta
     3481 gacgatttaa ttctaatgaa tactgttttt ggttttttag gaaatatgtt tgtcaaagca
     3541 ctcctatcaa ttttactcat tgtaaaacgg aaagatttat taaagaaact agggttagga
     3601 gctgcagatc aaaccataaa taacacgaaa aacatagcaa gtcattcttt gtctcaaatg
     3661 aaacaactgc ctggtcaact atcaactgct gggcaaaatt tggcaatcaa aggtgcagga
     3721 ttagcaggga atgtgtaccg agcaacgaat ccactcgctt ctgcagcgat acatggattt
     3781 caaaagtttc aacaaaaacg tacaccacaa gaatcggtaa aaggggggca agtgattaat
     3841 ccctttgaac taggtgggcg ttcaaatcaa gacttgcctt atagtggtcg tcatgttcgt
     3901 tatcatgggg tagagcctga aatgtcttct accaaatcaa aaacagagtg gaattctggt
     3961 ctttcaaatg gtttgattcc aaattcgtcc atgaattcag taagaccgaa acaacaggta
     4021 caatcgttgg cacaatcgag taagcaacaa gggaataatc aaagaaaacc attgttaaat
     4081 aaaggtatcg cttttgttgg tcaatcgatt ccgttgcctg ctggaaaagt acccgtgcca
     4141 caattaacag ctgctggttc tctttcacaa cagattcacc aagtgaaaaa tcaggcgaaa
     4201 aatgggagtt atccagcaat gcagcagtta ttgaatcaat actctgttcc taagtcgtcg
     4261 gtagccactc tatcaggaaa tcgttctgtg caacgacaga tacaacgttc gtctgaaagt
     4321 aaaccgtcga tttccgtacc aaaatcctta caacctgcat tttcttcttt taataatcca
     4381 gtagagcaat cgaaaaaaat agaaaagaaa aatgaaacat tgccgatttt ggacgcaaaa
     4441 ccaggaaccg attttacttt accaaaagga cgaacagttc aaaaaccatt atcgaagaaa
     4501 ggatgaggaa gaaatgaaga aagggtgtct aggttgtctg cttttcttta ttcttccctt
     4561 ttttctattg ccattctttt tttcaggaga actttccgag gatggaggag aggctacagg
     4621 tctagctgtc gcttttaatt gggaatatac cgaaggatta ccgatttttt cagagattaa
     4681 aggacgtggt ccgatcacag atgaacatgc gcaagcagca gtcggtgcag cggtaaagta
     4741 tcagttatta ccaagtgtga ttctttctca attaggttat gaatcgactt atggacaatc
     4801 acaatctgga agaaacgata caaacttctt tgggattacc tggtttcctg gttgtcccta
     4861 tccacaaggt tctgcacggg ggattggtgg ttctgaaggt ggcttctata tgaagtttcc
     4921 gaacctgaaa gattgttatg actattacgg ctttttggtg gcgagtcaaa gcaactttaa
     4981 tcaatgtgtg ggcaataaag atcctgggag ttgtttattg attctaggtc gtggaggcta
     5041 tgcggcggca ggtattaccg aaggcagtcc gtattaccaa ggagcgatga gtatcataaa
     5101 gtcttataac ttaacagaat atgatacttt tgcgatcaaa cattggaata gcctaccaac
     5161 aaaaaaatcg tcacttgttg cagggaaaag taaaggtaat gcgaaagttt tagatcggat
     5221 cattggacga tcggtaaata atggagagtg ttatggtggc acggcgtggt atgtatccca
     5281 a
//
LOCUS       DACEPW010000149         5224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEPW010000149 DACEPW010000000
VERSION     DACEPW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.152"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..75)
                     /locus_tag="IW819_002177"
     CDS             complement(<1..75)
                     /locus_tag="IW819_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1245269.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYN"
     gene            complement(273..1700)
                     /locus_tag="IW819_002178"
     CDS             complement(273..1700)
                     /locus_tag="IW819_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HAQ1245270.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
     gene            complement(1845..2393)
                     /locus_tag="IW819_002179"
     CDS             complement(1845..2393)
                     /locus_tag="IW819_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245271.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2394..3251)
                     /locus_tag="IW819_002180"
     CDS             complement(2394..3251)
                     /locus_tag="IW819_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1245272.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(3537..3824)
                     /locus_tag="IW819_002181"
     CDS             complement(3537..3824)
                     /locus_tag="IW819_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1245273.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3814..4143)
                     /locus_tag="IW819_002182"
     CDS             complement(3814..4143)
                     /locus_tag="IW819_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1245274.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(4220..4492)
                     /locus_tag="IW819_002183"
     CDS             complement(4220..4492)
                     /locus_tag="IW819_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1245275.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(4492..4755)
                     /locus_tag="IW819_002184"
     CDS             complement(4492..4755)
                     /locus_tag="IW819_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1245276.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(4861..>5224)
                     /locus_tag="IW819_002185"
     CDS             complement(4861..>5224)
                     /locus_tag="IW819_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245277.1"
                     /translation="FIPEDFQNLQLENMRLEDLFRLADDDKYRFEVLKIKKNELLNKL
                     YPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYVEDEVI
                     IKYMKPVIKLVNKNKGNG"
BASE COUNT         1562 a         1007 c          739 g         1916 t
ORIGIN      
        1 attgtaacgc aggtagtaac caacagcgac aataatgacg tcttttttga attgtttgcc
       61 tttaaaatga ttcattactc tgtcctctct gtcttttttc tcaattttac actaaaatag
      121 attttttgga aaactttgca acagaaccac cgttcctatg ctcatttttt ggatttttta
      181 atcacgtaac cagaaaaata aatcccctct atttcacgca aatcaaagaa gaactaagta
      241 actaccgttt aaaggtagtt acttagttct tctcagactg tagacaaaaa ggagtggctt
      301 aattgccaac tcctttttgt tagtctttgt agaaatttta aataaaaagc cataggcttt
      361 tttgctttcc atagccatgt ggctagtttt tttaaattca tggcagcaaa cgtaagcatc
      421 gtgtgcgtgg cgactttttc gagacctcga tatttcgtcc aacgcatacc atgcttttct
      481 tttgcatctg caaatattcg ttcaatggtt tgttttctct ttttataaat ttctctattc
      541 aacgccgtat ggcgttggtg ttcaagttca tccataagat ccgcccaaac atgtcgagtt
      601 actatttttt gatgttttgt actcaaagta cagtttttaa gtaatggaca actcctacag
      661 aattttggat ttgatttata ggttcgataa ccatcacgag tgatagttga aaataacaac
      721 ggttgattat tgggacataa atagcagtca aaataggcat cataaacaaa gtctttttta
      781 cgaaacattc cttttactcc atgaggtaca gtatatggaa gaatcgggta gagtttcaag
      841 tgtgacagaa aatgaacaaa tttaggcgtc ttatatcctg catcagcgac aacacgcttt
      901 atatttggaa aagctctttt cgactgtttg accagatcga ttgcgacttg gctgtcatgt
      961 acatttcctg gtgtgattaa tgtagataag ataaaaccat tatcttcaca ggaagtatgc
     1021 gcggaataag caaattgttt ttctcgttca cctttaacat agtaaccact ttctggatct
     1081 gaatgactta ttttttgaag actcaactca ctttctgtgt cccatgtaaa aggacgtttt
     1141 cctacagcaa tacgttcttt atttatttca tcttctaatt catcttgata agctttagct
     1201 tgaccgtagg tcatttcttt tgtgaactta tgtttattgg catttgcctt aatatgagta
     1261 gaatcaatat aaaggttgtc ttccgtaacg aagccagctt ttacagcttg ttctagaata
     1321 tgagagaaaa tgtcttcaaa taaagttgtt tctctgaatc ttcgaacata gtttttacca
     1381 aaagtagaga aatgcgggat aggatcctcg aatgaataac ctaaaaacca acgataagca
     1441 acatttgttt caacgtcttt aatagtctgt ctcatcgaac ggataccaaa taaatattgg
     1501 ataaatacta atttaattaa gaccactgga tcaatactgg gacgaccatg ggtcgaatac
     1561 gtagattcta caatgggata aatgaaatca aactgaatcg cagcatcaat ctttcgaact
     1621 aagtgatctt taggtacaag atcatcaatt gttaacatag caaattgatt tctaccttca
     1681 atcgattgtt ttctcatcat tagaatcact cccagaatag tttatacaat gagtataaaa
     1741 caaaaaaact gcaaacaaca ctcccaattt aggagtttgt ctacagtctg agaagaacta
     1801 agtaactacc gtttaaaggt agttacttag ttcttctttg attattatct aactttatta
     1861 ttcattcccc accctttgcg cagtgagtct tcggtatctt gttttatctg actggtagtt
     1921 cttattattt ctccacgatc atctaattct ggaacaattg gatatttttc tctgaacaag
     1981 tctagttctt tcaccacgtc attaaacaaa tcttgcttat cttcttgcgt aagattctgt
     2041 ttaatatact catctaaagc ttttctaagc aattcatctc ttgtcatatt agtctcatac
     2101 ttttctacta aaactctgga agtcgcattt aacaaagtcg acagttttaa aggatgcttg
     2161 gtggaataaa ccttatcaaa ctttctgatt ggtgcgccaa ctttttttgt tcttcctcta
     2221 ctctgtcttt caacattgga ggaagttttt atttcttgaa cttggtgcac ttctaattgc
     2281 ttattttcag atggtgtaat tgcttgctgc tttttctttg cttctgcttc tttttttatt
     2341 ttggcgagtc ctttgttgtt cccaaaaata tcaagtcctt tgatgtcagc caattactcc
     2401 acctccgcat accaattctc gtgttcttcc aattctttca aaatactgat aaataattcg
     2461 tggcttttgc gatcccattg ttttaagaga cctttctttg tcttctccat cgtaatacct
     2521 gatccgtcgt atcgttttaa gcgcgcattt ttgagtacaa catttttaat caaatgttcc
     2581 ccataaatct cttgggcttg tttatacatt tcctgatcta ctgcgtcacc gctttccaac
     2641 atgaaaggaa taacaccagc aacatataaa tttgtatcaa attctttcac aaaaaagtcc
     2701 atatactcaa gatattgcat cgcatttctc atcgatttta cttgggtctg taagacgacg
     2761 attgcccagt ccgcaatgta catcgcggat ttactatata cagaaataga aggtgggaca
     2821 tctaaataga tgacgtcaaa ctcatcaaat aatggttcta gcttttcttt catatatttg
     2881 aattgggata atgaatcatt atattggtct ttgatccaat catcatacat ttcaaaggac
     2941 atatctgctg gaagtatata gaggttattg attactttat agacagcctc tttaggtgga
     3001 agattgttta tcaacacgtc agtcacagta ttttcgtaaa caatcggttc accttctccg
     3061 cctccttctt cgttagtgaa gttcatatgc gttaagttca tcacatcaga agtatttcct
     3121 tgcatatcca agtcaattat caaggttctt tttccttcat taactgctga ttcaaaccct
     3181 aacatttgcg cgcacgtact tttacctacg ccacctttaa aattaccaat tacatatttc
     3241 ttcgctacca cgttcctacc tcttttcttt tagcaatatt catttgatac tattataaca
     3301 tatttatttt tagttactac caaaaaaaag atatatatgt actaccttat aattaataag
     3361 taaaaggtag tacataaata aggaaaagtt gaaatatatt gatttatagc tttcctcaaa
     3421 gtaatgacgt tatttaattt atcgagaagc agagaatact cgcgacttta gtcatgtaga
     3481 cgaatcagat tttcgataaa aaagaagccg tccttcttaa gaacgacaac aaatcactag
     3541 attaattttt tcaactgttc ataaaagttt tcatgcgttc cacataacaa tactggaata
     3601 accttgtttt ctataatctc gtaagctact ctatatgttg tgcccgcata tttgaaccca
     3661 ctagaccaaa tacctgataa atccccagtt ttttgttcac ccttatatgg atcaatcgca
     3721 atttcatcat aaattaaatt caaaaatttt tctttcaatg gcttctcttt tagctttttt
     3781 aaatacttaa tgacatgttt tgaaggaacg atttcataag ccaatttctg ttgccaactc
     3841 ctctttagtc attactttag aattagtaag tgtatcttca gcaatatctc taaaggaaga
     3901 atgcattttg ttttttctat tcttaaattc tacaagaatc tcatctttat cgtagccttc
     3961 tgcaattata tcagacaaaa tatcttcgct aaaatcataa aattcttttt gtggttcaac
     4021 aaattcataa agaataccat tctctaccaa ctttgcttca acttcgactc cattaggaac
     4081 attaaaatct ttaggtactg tcaacatgat tgaattgcct tgttttcttg tttttgtaat
     4141 catttaaatc cctcatttct aacagtatgt aattacttag taaatacatt ataccacgag
     4201 ataatgtatt tacaagtaat tactgtgact cgtaatgcgt ccttgctgaa taaatttcaa
     4261 ccacgtgttc atcttcatct accttataaa caacccggtg ttgtctattc aatcttctag
     4321 aatacctacc ttcatatgta ggttttaact tctcaaatga ttgggtaggg agatagggat
     4381 cttcctttaa agtctgaata accttctcaa attgaggttt caaattagac tgtttgattt
     4441 tctttaaatc agctttagcc gagtttttaa tcatcacaga atagttattc attataagtt
     4501 atcccaatca atttcatcaa cattagtaaa tccactatca tctttttctc gcttacgaac
     4561 aacacccaaa gtgcctattt gctcaagtaa tagcgtttct ttgattgctt tcaaatcatc
     4621 caaaccaata agcaccgcat tattttcact gcgctcacta atgatttcaa tttccgtatg
     4681 attctcattt acatctttca acaacttata aaaatttttc cgtgctgagc taggatttaa
     4741 gactgttttt gccatatcta ctccccctct aatttatatc tccattctaa cgtacttaat
     4801 taagtacgta aattcgattg ttttgtttaa attggagtac caaaacagca ttgtaaagtg
     4861 ttatccgttt cctttatttt tattaacaag ttttatcaca ggcttcatat atttgattat
     4921 aacttcatct tctacgtaga tatctgttat cattacgaca tctgtactat ttcgattgag
     4981 tactttcact tgatctccag cattgatttt ccctctaaat cttttaggaa tgaaccaact
     5041 atacatatga ttagcatcac caaaatgtgc ttttatcgca ttctcaggat ataacttatt
     5101 caatagttca ttctttttaa ttttcaaaac ttcaaatcta tacttatcat catctgctaa
     5161 tctaaataaa tcttctaaac gcatgttttc taactgtaag ttttgaaaat cttccggtat
     5221 aaat
//
LOCUS       DACEPW010000150         5219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEPW010000150 DACEPW010000000
VERSION     DACEPW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.153"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            119..625
                     /locus_tag="IW819_002186"
     CDS             119..625
                     /locus_tag="IW819_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1245278.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="IW819_002187"
     CDS             642..1541
                     /locus_tag="IW819_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1245279.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIID
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="IW819_002188"
     CDS             1628..2512
                     /locus_tag="IW819_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1245280.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="IW819_002189"
     tRNA            complement(2704..2775)
                     /locus_tag="IW819_002189"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="IW819_002190"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="IW819_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1245281.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGITSSSKRKRYDN"
     gene            4372..>5219
                     /locus_tag="IW819_002191"
     CDS             4372..>5219
                     /locus_tag="IW819_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1245282.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEV"
BASE COUNT         1848 a          943 c         1079 g         1349 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcataga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcacgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagt
//
LOCUS       DACEPW010000151         5216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEPW010000151 DACEPW010000000
VERSION     DACEPW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.154"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2129)
                     /gene="fss3"
                     /locus_tag="IW819_002192"
     CDS             complement(<1..2129)
                     /gene="fss3"
                     /locus_tag="IW819_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1245283.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFT"
     gene            complement(2546..3712)
                     /locus_tag="IW819_002193"
     CDS             complement(2546..3712)
                     /locus_tag="IW819_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1245284.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(3767..4417)
                     /locus_tag="IW819_002194"
     CDS             complement(3767..4417)
                     /locus_tag="IW819_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1245285.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            4492..4692
                     /locus_tag="IW819_002195"
     CDS             4492..4692
                     /locus_tag="IW819_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1245286.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            4704..5030
                     /locus_tag="IW819_002196"
     CDS             4704..5030
                     /locus_tag="IW819_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245287.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
BASE COUNT         1584 a          915 c          899 g         1818 t
ORIGIN      
        1 atggtaaaga catactcact cttcacaaag tcttctttgt tttccttaaa agtagaacgg
       61 atttctaatg gcgtaatctt ttgaaaacct tctggggctt ctacttcttc aagtaaataa
      121 tccccataag gaagattttc aaacttgcca taaccatcaa accctaattg ctcgttataa
      181 gctgtggtcg cttcatcttc ggcacctgtg atttcattgg tcccttccaa tggcgacact
      241 ttaaaggtca gatcattgaa gccagtttcg gtagtgcccg cagctgatcc agcaaattta
      301 aagaaatcaa agccaaagcg aatcacttgt tcttttgccg taacatttcg ggtaatcacg
      361 gcattttttt cgctatcatc cacctttttg atggatacag gatactttgt ttcatcaagg
      421 ctatatcctt cgggcgcttt tgtttcttgc caataatact cgttgatagc taaatgttta
      481 acggcaactt ggttcttttc gtctaaagtc aaagtgaccg tttcatcaga acctttcgtt
      541 cccttcgcta actctggttt aaaagcctca ctccatttaa cagcttttcc atccttagca
      601 gtaaacaagg tgtattcagc accctcaaag actgccttac cttgtgcttt atccccagta
      661 tctttgtctt ctttcgtcaa agtggtttcg ccagtgactt cttgattttg cccttttacg
      721 ttactggtaa caagggctac ggtttgattg gcatacttca attcgacttt tacaggtttg
      781 aacgtattca cgaacccatg actagcttta gtttcggtca cataataggt tcccaattcc
      841 aacgcattag caatctcttt tggtgtttcc gcatgaccgt tttcatccgt ggtcatttct
      901 tgaacgattt caccggttgg actgtctttg cgaatggcaa acgtattacc agctaaggaa
      961 taattgtcat tccaaagatc actccctgtt tcaactccgc ttttatccag aatgatttgc
     1021 cctttttccc gtgcgttttt tgaagtcacg taaatggttt cgcctgcttt aatggtggta
     1081 gtcatggggg tggtatcaat cgtataaggc gccggtactg atttttcggt aatggttact
     1141 tttgtaccat gaggaatctc atccaatgta gcaatgcctt ctttgtcagt agtcacgtct
     1201 tttgcaggta aagtttttcc aaagtctaaa tggaaaaccg ttcctggtac aacatcaccc
     1261 gattctttat cgactttttt gatctttaaa gaaccctccg tttccacatc aattttgatt
     1321 gtgtaggtat taggtttatc aatcgccccg gccattaaag tctgttggcc agccttttta
     1381 taggcgacag gtgttccggt accagcagat ttcttaagcg tcagcacacc actttctttt
     1441 gaattggcat ttggggtgat tatcaactga ttaccattca cacgataatc aatgtttgcg
     1501 gcattttcca caacctcatc gaactctgac aaattcagat tattcgtatc cgtcacagta
     1561 gtcgattgcc ctaatactgt tttagccgta ctattatgga aggttggttt cttctgataa
     1621 tcgtcaatgg cttgatttat ttttgcctca atggctgcga tatttacggc actaccatct
     1681 gatcgtttta ttgagtgaag ggtataccca ttaacttctt gccagatcat cttctgtgcc
     1741 acaatatgag tatctgcatc tgtacctact tttttccata aaacggaaac taatttggct
     1801 ttatcggaca tatctggtaa cgggtttttc tcatagccag gagtgaactc attgtaaatc
     1861 gggactcctg gttcaataca gaaaatatct tgtttaaagc cgttgtacac tgcataatgc
     1921 ggaacagtga cttctgacat tggacgacca tcactaaaag ttccttcaac agcatattga
     1981 tgtgctaaat ctagttctac ggttaccgtt tgtggatggg taatttcatc agcaaaagta
     2041 atcgtaggag caattgctcc aacaattgct tgaacagcca aactcaaaat agttaataca
     2101 cataatttct ttttttgttt tttatacatg aaatgttctc ctttcaaatt aagcacaaaa
     2161 aaataagatg ctcgcttgaa catctttgtt tataaagtct ttttaaaatt tttattgact
     2221 gaatagaatg gatttgagaa tgaatgagcc taaaaattta atctaaaata cgaaatatac
     2281 tgcctttaaa ttagaacaca ttttagaaca caaacatttc aaatttaatc aaatataata
     2341 aaatatattt acgggaaaac ctttataaat caaggttttt caaacttatt caatgatacg
     2401 aagaaaaaac caggtcttaa gaaagcacgt aaagcttcac agttttcaaa acgttaatcg
     2461 ttgttatacc aacgatttca gacactttct cattgcgaga aggtgtcttt ttttcgttct
     2521 ggtaatgaga aaggtaatga caatattata aaccagtgta agcaatgaat ttttctaacg
     2581 catttcgttg agctttttca gtaacatgag aataagtgtt aagggtagtt tcagcatttt
     2641 tatgacccaa tcgatcttgt gtttctttga tacttgctcc accttctagt agaagagtag
     2701 cgtgagtgtg ccggaatcca tgaatagtaa cgtgcttaaa tttatatttc ttctctaaac
     2761 gtttcaatat attatttgaa tagtccgcat gcaaaggttg attaatgtca ccgtctgcat
     2821 ttacataggt aaagataagt tgattttttg tatttgtatt tatgccaaac tgcaaaagct
     2881 ctcgagcttg ttcttttttc cagattttta acatttcaat agtttgtgca tcaattggta
     2941 atattcgttc ggaagatata ttttttgtat ttttttgaat cctgacagtt tttcttcttg
     3001 cattcttctc ttcatttcga atagttgcat agctctttct aacatacatt agctgttctt
     3061 caaaataaat gtcagaccaa tgaagagcag ctagctctcc tttacgtata ccagtagtaa
     3121 tcaaaagctg gaagattaaa atatgtttta aaggctcttc tttattgaga atatcccaaa
     3181 aagcttttag ttcttctaat gaaaagtaat tttcattttc tttcttttcc cgctttggta
     3241 gatttgtatt tgccatagga tttcggtgta atagctctgc aaaaatggca tactcaaata
     3301 tttgagagct gtaagactta agctgtttga tattcttgaa ttttaaagcc cattcattga
     3361 ctgcattctg acagtgaaca ggagtaatct tagaaatgga cttattcccg aattttggta
     3421 gtatatgcaa gcggaaaagg tcttttgtac gttgatatgt cgtttcttgt acacgattcc
     3481 tataagaagg caaccattct tcatacactg ttataaacaa gtcattagaa ggcttatgat
     3541 agccggtatc ttcaatctcg accttttttc ttgcttcgtg taacttggct tctttttcag
     3601 ttttaaaccc tttttttaca tagtattctt gctttccatt ttcatcttta cgaacataca
     3661 agcggacact atatcgtgca ccgctttttg ttgtgtactt ttgtattgac ataactaatc
     3721 tctccaatct gataacttgg cgagttggct gattgaataa ttagttttat tgaagttttg
     3781 atattaatcg agctgttatc tctcttttta gattgttaac ctgtacttgg gttaagttgc
     3841 caaataactc agattctaac tcttttcttt catcgatatt taagtttaaa gctttattaa
     3901 taaacttctt ttgtttggca aatagaatcg tgtattcaaa ggagtcctct agaatacttt
     3961 ttaaaaaaag aaagtcggag tcatcttctt gaagtttgac ttcaagtaaa gaagtcatgg
     4021 aaaagtatag attagataat tcagtaaaat aatctgagtc aagactggca atattcttga
     4081 ctattttgtc ctttttttct aaacgagcag tttttattct atcaagatta ggttgttcta
     4141 tggaatttgt ttcgtctaat cccatcaaat aacttacagg aacatggaaa taatcagcaa
     4201 ggatattcca tgtttcagta tttctaggat ttcgctcacc acggtaatag ttcgtaagag
     4261 ttgtatagtt aataccagtt tctgtactaa tttgtttaat ggtcttttta ctggaatcaa
     4321 tataaaattt tattctattc aaagtaacgc cccctatagt aaattccaaa ctattatagc
     4381 atgaaaacca caaattgagt aatattttaa ttgacattac tcgttttgtg gtttaaaatg
     4441 tgcttattaa gataaaaacc acaaagtgaa taatttgaaa ggaggcgctg aatgataact
     4501 cttaatgatc aattcattag aagcttaaga agacatagag ccgatcttat acttactaaa
     4561 aacgatgcag caaaattgat aggtataaat cggaaaacat atgtcaaaat cgaaaatggg
     4621 agcaaagaaa gtattagagc atctacttat caaaagttgg taaattggtt acttaatgat
     4681 ttgaaaatct aaataaatac aacttggcga gttggctgat tgaagaaata gaaaatgaga
     4741 ggagaaaaat aatggatgca ggaattacag taatgttaga taaacaacaa acgaatcaat
     4801 tgaaaaatta tgtttttgaa atgacaaaag aggctattga tcaagcaaga attgataccg
     4861 gattagaaag accatttcta aaaggaaaag agatggctaa atatttgaat gtatcatata
     4921 caactttttt gaaatttaaa agaatgggat tgccagttat tttattagaa aaaatggagt
     4981 tgttttcaaa agaagaatgc aaaaaatgga tattgagtca tcaaatttaa aaaacacctc
     5041 gttttttaaa tttgatgaag tagaaaaaat taaaaagagt gttcccgtca gtttgcagat
     5101 cgggggaaca ctcaataata aagttctgag aaaactttat ttgaatctat tatagcaaat
     5161 aaaatcagaa gcaggtgtga caatgaatga aaaagaagtt gaaagcttag ttgaaa
//
LOCUS       DACEPW010000152         5188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEPW010000152 DACEPW010000000
VERSION     DACEPW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.155"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..433)
                     /locus_tag="IW819_002197"
     CDS             complement(<1..433)
                     /locus_tag="IW819_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1245288.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLG"
     gene            622..1383
                     /locus_tag="IW819_002198"
     CDS             622..1383
                     /locus_tag="IW819_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1245289.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(1498..1938)
                     /gene="fabZ"
                     /locus_tag="IW819_002199"
     CDS             complement(1498..1938)
                     /gene="fabZ"
                     /locus_tag="IW819_002199"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1245290.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2158..2913
                     /gene="fabI"
                     /locus_tag="IW819_002200"
     CDS             2158..2913
                     /gene="fabI"
                     /locus_tag="IW819_002200"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1245291.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3023..4036)
                     /locus_tag="IW819_002201"
     CDS             complement(3023..4036)
                     /locus_tag="IW819_002201"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1245292.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4201..5121
                     /locus_tag="IW819_002202"
     CDS             4201..5121
                     /locus_tag="IW819_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1245293.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1586 a         1031 c          941 g         1630 t
ORIGIN      
        1 aacctaataa attatccgtg tttggcgaat aactcatgat gttgtcaacg taattcgtgc
       61 ccgaactcat attgatctcg attttgatac caaaaggatt gcgcgtcatt tttgcttctt
      121 ctgctcctgt gaatccgata tctaagcgga ccccgtaggc acccatctta tggaaaaaag
      181 ataagtcatc gtaagaaatc tccaactgtt caaataacgc tggattgata tcaaccatta
      241 cttccatgcc taggctattc gcgtaatcaa ctacttcttt aaattctgaa agaacttttt
      301 ctttatcatc attgatctgc agcaaactgg taaaaacccg tttataccca tacttatgag
      361 ctaaatccaa gtacgctttg tccttttcaa atgttgaccg ttctggataa atcgagattc
      421 ctaattttcc catattacca tttcctcctg catcttttcg tttttgtatc cacttacatt
      481 atagcaaaca caaagtaaat ggtctacacc ttttcttttt cttttaaaat aaatgataaa
      541 ttccaataat ttaagtaggt attttccctc tattttgtta tcattaaaga taacaaaata
      601 gtaaattgga aaggaacagt tatgaagaaa aataaagtta ttttaagtat tagtttagca
      661 gcagcattgc tgtttgctgc tggttgttct gcaaacaaca cgacttcttc tagttcgtct
      721 acagaaagtt caaaaagtac atctactgcg gaaagcacga ctgccagtag cgtcgatttg
      781 aacgcattgg aattaccaca attgacgaaa gatgtcgccg aaaatgaaga tctggtcgaa
      841 atggtgacga ataaaggcac aatcaaaatc aaattatttc ccgaacacgc accaaaagcg
      901 gttgaaaact ttatgacaca tgcaaaagac ggttactaca acggactgac tttccatcgc
      961 gtcatcaaag atttcatgat ccaaggtggc gatcctaaag gcgatggcac tggaggggaa
     1021 agcatttggg gagaaggatt tgaaacagag atcaacaacc acctttataa tataagaggt
     1081 gcgttatcta tggctcgctc acaagatcct aacagtaacg gcagccagtt ctttatcgtt
     1141 caaaatacag atgatatgca tgatggtctt ttaaaagacg actatcctca agcaatcatc
     1201 gacgcttata aaaacggcgg ttatccttca ctagatggcc attatactgt cttcggccaa
     1261 gtgattgaag gaatggatgt cgtggatgct attgcaaata tcgaaacaga tagttcagac
     1321 aaaccaaaag aagaagtaaa aatcgataaa atcaatatac tgcaagaagc aaaacaaaaa
     1381 taaaaaaata acgtctgaat cctgcataga tgacaacgta tgctaggatt caggcgtctt
     1441 ctttttacat aaaaagcaag taagcttcct tttgttttgc tatatcaggt tttatagtca
     1501 agcattttgg ttgacgatga acgtgaactc gcactcacag acttttttat catctacata
     1561 tgctgcagat aacgctgtac cgactgatcc cttcattttg gtgatttcaa aatgacattt
     1621 cagcacatct cctggaacaa ctttttgacg gaatttggcc ttattgatcc caccgatata
     1681 agctcttttt cctagaaact cttctgattt taggataagt atcgaacctg cttgtgccag
     1741 tgtttctaga atcaacgcac ccggcatcac tggattgtca ggaaagtgac cttggaaaaa
     1801 atcttcattg attgttacat tttttgtcgc aacgatcttt tttcctggtt ctaaagtatc
     1861 tacataatca atataacaaa taggataacg attaggaatc aattccatta cttcagtagc
     1921 agataatacg taagacataa ttaaactcct tctttcaggc aattgccatt ttcattactt
     1981 tgactatcaa agtatcatat tcctaaatat ttagcaatct ttttttaatt taagaaaaaa
     2041 tagtttgaca atcaaaacat tttgactaat aattaagaaa tcaagaagtt attttagcta
     2101 ttattctcta tttttttacg actatcaaat ttttatcaga aacaaaggag agtcattatg
     2161 tcatttctag aaggaaaaaa aatcgtcatt atgggcgtcg ctaacaaaaa aagcattgct
     2221 tggggttgtg ctgaagcaat gttgaaacaa ggggcagaaa ttatctatac ttaccaaaat
     2281 gaacgaatga aaaaatcatt agtcaaattg gtcggcgaag aagcattcac tgtagaatgt
     2341 gatgtttcta gcgacgaaag catcgaaaaa gcaatgaatg ccatcggtga atatgctggg
     2401 gagatccacg gccttgtcca tgcagttgct tacgccaaca aagaagaatt gtccggaaat
     2461 gtgtctgata tttccagaga cggctatcaa ttagctcaag atatcagcag ctactcttta
     2521 attgctgtat caaaatacgc acaaccgttt cttgcaccaa acagcgggat cgttagcatg
     2581 acttatctag gttcagaacg cgcaatcccg aattataaca tgatgggtat tgcgaaagct
     2641 tctcttgaag ctgctatccg ttatttggca gctgagttct cgcctaaagg aattcgggtc
     2701 aatggaatct cagctggggc aatcaaaaca ttagctgtca caggtgttaa agactatcaa
     2761 aaactgatcc agttatctga atctcgtacg cctgatcagc aaggtgtgac gatcgaagaa
     2821 gtcggtaatg cttgtgcttt cttaatcagc ccactttcca gcggaatcat cggtgatatt
     2881 atctacgttg acaaaggtgt ccatctttca taaacttttg ttgcattctt taaagtaaaa
     2941 catataaaaa tcaggaaaaa actttttaaa gtctcttcct gattttttct ttttacgttt
     3001 ttttcaactc attactttga aattattcca cgacttcaat ccagccttct ggtgcttcta
     3061 tgtctccaaa ttgaatacct gtcaattcat catacaaccg ttttgttact ggtcctactt
     3121 ccgtttcact ataaaataca tgaagattgt tattgtgata aatcccaccg attggagaaa
     3181 taacagccgc tgtaccacat gcaccagctt ctgcaaattg atctagatca tcaatgaaaa
     3241 tatctccttc aattgcttgc agtcctagtc gttcttcagc taactgcaac aaggaatatt
     3301 tcgtgatact tggtaagata gagggcgatt gcggagtgat gaattttccg tcttttgtaa
     3361 ttccaaagaa atttgctgca cctacttctt cgattttcgt atgtgtggct ggatcaagat
     3421 aaacacaatc tgagtaatcc agttcttttg cttctgttcc tggaagtaaa ctcgctgcgt
     3481 aatttccgcc gacttttgct gcaccagtcc ccttaggagc agcacgatcg taatcagata
     3541 caataaaatt cgttggtttt agtccacctt tgaaatacgc tcctactggc gtacagaaga
     3601 cgacaaataa atattctgga gctggatgga caccaatgtt ttcaccgacc ccaatcagta
     3661 aagggcgcaa gtataatgta gcgcctgttc catagggcgg cacaaaatct tcatttgctt
     3721 ttaccacttg cttgacagca tcaacgaaca tcttttcagg gaccattggc atcagcaatc
     3781 gtctagcact ttgattcatc cgtcgactat tttgttcagg tcgaaagaga ttgaccttgc
     3841 cgtcttttcg acgataagct tttagacctt caaaacattg ctgaccataa tgaagcgctg
     3901 gagagccttc atgaatcgtc aggtaattat cttctgtcaa ttctccttct tcccattgtc
     3961 cgtctttcca tttggcaata aaacgccaag gagttttgat ataagaaaaa cctagattgt
     4021 tccaatcgat ttgcatatac ttaccacctt cgttattttc tccacatttt atcattatcc
     4081 atattatttg tcattttatt ttcagctttt ttccttcatt atagttattt ttgctatttc
     4141 cttttttgaa aacttcttgg tacaatacag aaagaatcaa attttagaaa agaggatttt
     4201 atgttttcat tattagcaac tgccggtacg acagactctt ctggcgagac attgacagat
     4261 caagctgtca gcaaagtaaa tgcgtttcag cggttttggg acgggattga ttgggacgcc
     4321 attttggctg cctttatcca aaaaagttta tatctgattt ttttgatcgt ccttttttgg
     4381 attcttcacc gtgtgggaaa atatctgatt gataagtctt ttaatcagta ttctaaaaaa
     4441 gccattctaa acgagagccg cttaaagact ttacatagtc ttttaaatac tatttttcat
     4501 tatacccttg gtttcttttt tatctatgca attttatctg ctatcggtgt gccgatcgga
     4561 tcacttttgg ctggtgccgg aattgcaggt gtggcaatcg gtcttggtgc ccaagggttc
     4621 atgagtgatg ttattaccgg attttttatt atcatggaac aacagatgga tgttggggac
     4681 tacatcaagc tagcaaactt aacgattgaa ggaaccgtcg tatctgttgg tatccgtacc
     4741 cttcagctga aagcaacgga tggtactgtc catttcatcc cgaatcgaaa catcaccacg
     4801 atcagcaatc tctctcgtgc aaatatgcaa gtacttttag atattcggat tgttcctgaa
     4861 gaaggatatg acaaaatcta tgagatcatt gatcgagtaa atcgtacatt agctgaaaaa
     4921 taccaagaag agctccaaac tgaaccttct atctttggat tagtggatct tggaaacagc
     4981 aattttgcga ttcgtacggt ctgctacgca ttgaacggaa aacaatatgc cttaaaagaa
     5041 gagttcttga gctcctatgt caaagaattg acagcttctg gtttcacgat tccgaacagt
     5101 ccaattgctg tcggaaaata atttggacaa acgatgcaac gaatctcact aaaaagagcg
     5161 tgggacaaaa gtaaagaaca cttttgtc
//
LOCUS       DACEPW010000153         5155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEPW010000153 DACEPW010000000
VERSION     DACEPW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.156"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(46..366)
                     /locus_tag="IW819_002203"
     CDS             complement(46..366)
                     /locus_tag="IW819_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1245294.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="IW819_002204"
     CDS             complement(514..2748)
                     /locus_tag="IW819_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1245295.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="IW819_002205"
     CDS             3227..3493
                     /locus_tag="IW819_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1245296.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..>5155
                     /gene="ptsP"
                     /locus_tag="IW819_002206"
     CDS             3493..>5155
                     /gene="ptsP"
                     /locus_tag="IW819_002206"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1245297.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELAD"
BASE COUNT         1542 a          973 c         1032 g         1608 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttc
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatc
//
LOCUS       DACEPW010000154         5137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEPW010000154 DACEPW010000000
VERSION     DACEPW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.157"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            325..1614
                     /locus_tag="IW819_002207"
     CDS             325..1614
                     /locus_tag="IW819_002207"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1245298.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            1861..3111
                     /gene="clpX"
                     /locus_tag="IW819_002208"
     CDS             1861..3111
                     /gene="clpX"
                     /locus_tag="IW819_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1245299.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            3194..3781
                     /locus_tag="IW819_002209"
     CDS             3194..3781
                     /locus_tag="IW819_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1245300.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            4146..>5137
                     /locus_tag="IW819_002210"
     CDS             4146..>5137
                     /locus_tag="IW819_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245301.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRS"
BASE COUNT         1810 a          852 c         1054 g         1421 t
ORIGIN      
        1 gcaattgttt atcctgtcta tgatacatac atcgaagacc cgaagcgctg gtttcaactg
       61 actgtagagg cgtgtacagg aaaaacgatg tatgaaggta cagaagatga aaaacaagat
      121 tttttaaaaa aaagagaaaa aattttgcag caaatgatct tcttccatta gattttatca
      181 gtgaaaaaga taaaaatgtt atgaaaaaca aggattctat ccattcaata tgtttacaat
      241 ttgaaagggt tagtgtacta tttaaaggta tgcatgaaac gtttttgatg tataaaatga
      301 atttctaaac tcggagggaa ttaaatgact gcaacatttg aaaaaaaagg cacgaatgat
      361 ggtgttttaa aatttacgat tgcgcaagac gaaatccaaa aaggattaac aactgcgttt
      421 aataaagtga aaaaaagctt gaacgtacct ggattccgta aagggaaagt ttctcgtcaa
      481 gtattcaacc gtatgtacgg tgaagaagct ttatatgaag atgcattgaa tgctgtatta
      541 ccagaagctt atgaagctgc tgtcaaagaa gctggcatcg atcctgtttc tcaaccaaaa
      601 atcgatgttg aaagcatgga aaaaggtcaa gactgggtaa tcactgctga agtaacagtg
      661 aaaccagaag taaaattagg acaatataaa gatctagttg ttgaaaaaca agaccgtgaa
      721 gtaacagatg ctgacgtgga tgcacgtatc aaacgtgaac aagaagcaca agctgaatta
      781 gttatcaaag aagatgctgc agctgaaaat ggcgatacag tagtaatcga cttcgaaggc
      841 tttgtagatg gcgaagcgtt tgatggcgga aaaggcgaaa attactcact tgaattaggt
      901 tcaggctcat tcattccagg ttttgaagat caattagttg gtcataaatc aggtgaaagt
      961 ctagatgtaa atgtaacttt cccagaagat tatcaagcag aagatcttgc tggaaaagaa
     1021 gcagtcttca aagtgacaat ccatgaagta aaagcaaaag aagtgcctga attagatgac
     1081 gaattcgcaa aagatgttga tgacgaagta tcaacattag acgaattgaa agaaaaatat
     1141 cgcaaagaat tagtagaaac aaaagaaaaa gcagcggacg atgcaaaaga tgaagcagcg
     1201 atccgtatgg ctgttgaaaa cgcagaaatc gttgaattgc cacacgtgat ggttcatgat
     1261 gaagtacatc gttcaatgga cgaattcttg aacaacatgc aacgtcaagg tatctctcct
     1321 gaaatgtact atcaattaac tggttctaca gaagaagact tgcataaaca attcgaaggt
     1381 gaagcagaaa tgcgcacaag aacaaatctt gtgatcgaag caattgcagc tgctgaaaat
     1441 cttcaagcaa cagaagaaga aatcaatgca gaaatccaag aattatcaga aacatacaac
     1501 atgccaatcg aacaaatcaa acgcgtgttg acagaagata tgctggaaca tgatgtgaca
     1561 atgaaaaaag caatcgaatt gatcacaggt acagcgacag aagcgaaaga atagaaatca
     1621 agtaataaat catttctgaa gaagctactt ctagaaaatt gtttattcga aataagggac
     1681 tgtgacacga ttttgtcaca gttccttatt ttttgttaag agtaaaaaat gcaaaggtat
     1741 aggtcagttg atcctttgtt aaacactttt tctttttact aaactatgtt atgatagtaa
     1801 aagactgtaa agtctttgat agtttcttgc atactttttg caagttaagg ggtgaagacc
     1861 atgtatgaca atccaagtag caatgagacc gtacgctgtt ctttttgcgg gaaaacccaa
     1921 gaagaagtga aaaagatcgt agctggtcca ggggtctaca tctgtaatga atgtatcgac
     1981 ctatgtaaag aaatcattga tgaagaattt tatgaagaag ctgtacgcga attcactgaa
     2041 gtccctaaac cactagaaat tcttgaagta ttgaataact acgtgattgg acaagatcgc
     2101 gcaaaacgct cgttagctgt tgccgtatac aatcactata aacgtgtcaa tcagcaagca
     2161 caagaaaaca cagatgatgt tgaactgcaa aaaagcaata tctgcttgat cggacctaca
     2221 gggtctggta aaacattttt agcacaaaca ttagcaaaaa cattgaatgt accttttgca
     2281 atcgcagatg cgacaagttt gacagaagct ggttatgtgg gggaagatgt agaaaacatt
     2341 ttgcttaaat tgcttcaatc tgctgattac aatgtggaac ttgcagaaaa agggatcatc
     2401 tatattgatg agatcgataa aattgcgcgt aaaagcgaaa atgtatctat tactcgtgac
     2461 gtttccggcg aaggtgtcca acaagccttg ctgaaaattc tagaaggaac tgttgcaagt
     2521 gtgccacctc agggcggacg taaacatcca catcaagaat ttattcagat tgatacgacg
     2581 aacatattgt tcatcgtagg tggtgcattt gatggcatcg aaacgattgt gaagaatcgt
     2641 atgggcgaaa agacgattgg atttggtaca caaaaccaaa aactttctga agacgaaagt
     2701 gtcatgcagc atatcattcc tgaagattta ctaaaattcg gattgatccc tgagtttatc
     2761 ggacgattac ctgttatggc agctttagag aaattgacaa cagatgatct ggtacggatc
     2821 ttgactgagc cgaaaaatgc gctagtcaaa caatatcaaa aacttctttc tctagataat
     2881 accgaacttg aattcgaacc tgaagcgttg agagccattg cgaaaaaagc aatcgaacgc
     2941 aataccggtg ctcgaggact tcgttccatc attgaagaaa tcatgatgga tgtgatgttt
     3001 gatatccctt caaatgaaaa catcgaaaaa gtgattatca caaaagaagc agctgaattg
     3061 tctggtgagc caacagtaat ctataaagat tcagagaaaa aagcgggttg atgaaaagag
     3121 gctgccgtaa aagtcatttt ggcttttgcg acagccgctt gctttattca tcaccttgtt
     3181 aaggagagag accatgaaag tccatcaagc tgaaattatc ataagtgcag tggcaccaaa
     3241 acaatatcca gatacacaat tgccagaaat agctctggca ggacgatcaa atgtagggaa
     3301 atcttctttt atcaacacat tgattgatcg aaagaattta gcccgtactt ccggtaagcc
     3361 aggaaagacc cagacattga atttttattt gatcgagaac gcattacatt ttgtcgatgt
     3421 tccgggatat gggtatgcaa aagtatccaa aactgagcga gcaaaatggg gaaagatgat
     3481 cgagacatat ttaacgacta gagaccagct acgtgcggtc gtttctttag tagatttgcg
     3541 tcataagcct tctcaagaag atatccagat gtatcaattt ttgaagtatt atgatatccc
     3601 tgtaattgtt gttgccacta aggcagataa gatccctcgt ggtaaatgga acaagcatga
     3661 gagcgaaatc aaaaaagcgt tggattttga tcgcaatgac acgtttattc tcttttcttc
     3721 cgttacaaaa cagggaaaag aagaagcgtg ggcagcaatc gaacaagcaa ttgaatcata
     3781 aaaagaataa aaaagactta ccagtcaaac tctttcataa aagtttggct ggtaagtctt
     3841 tttttgagct taacttactt ttgtttcttg tcttttttct tttcattttg tggctgtggt
     3901 tttagaaatc gatcgatctc ttcttttggt gcaactgttt tttccggatc gacagcaaat
     3961 gattcgaaaa gtttatctaa attatctgta cgttcaaatt tcaaccccat tgtaaacacc
     4021 tctttgtttc tttttactta gtctatcatg taatcgtcca aattggtttc taagacataa
     4081 agttttagtt tttctttatc attggttata attaaattat aaaaagattt gaaagaggtg
     4141 agaaaatgat tggcgaagat aaattattga tgactatcca gctcacagac ttaaatgaag
     4201 aagaaaaaaa acaggaaata agcgattggg ctattttgac acgtctgttg atccagccaa
     4261 catttatccg ctctttcaca caacaagcgg atccgtatga tcttcgaaag cttcaagaaa
     4321 aaataatgct agcatcggta agagacgaaa gttggttagt tgtaggaaat gacgagaacg
     4381 aatgtagttt tcgcttggaa gataatcaat tattgataaa aaatgtattg tctatctctg
     4441 ttttcaaaga aaagcaattt ttgattcgag acttcattca gaaaaaaatg attgctcatg
     4501 gggtgttcgc ctatatgcga gcttatagcg agtttatcta tcacaataca aaacagatct
     4561 ctcaacggct attgtttgag aaaaaggatg aaatcgaaca tttacctaaa atgaaacaac
     4621 aaaacgggga agtagtagtc gattgcaacc aatttccagg atacgatctt ttttaccaag
     4681 gtttatgttt cacttcgtgt tgggaaatgt attatagtcg ctattatcat caaatcatcc
     4741 ctaaaccgat ttttctcgac atccaacaag tagaaaaagt gaaggaatta gaaaacgaag
     4801 tcatatgtat ccagctttat cgagacccct ttaactggca aaagccaaac aatcaaatgt
     4861 ttcaatccta ttttagggac cagcttgggt ttgatcatct tgcttgggac aatggtgtcg
     4921 gtttattgaa accgccattc gttgaataca tttacactga ccatacgatc caatctgtcc
     4981 agtatcagaa tgcacaaatg caacctgttc ctaaaaagaa tgcttcattt tttgtaacaa
     5041 aaagttatga tatccaacaa ggagattata aagaaaggcg tgttcgcgga acactaaatg
     5101 ctcaggcgta ttttccttgg gttgacgaga atcgatc
//
LOCUS       DACEPW010000155         5116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEPW010000155 DACEPW010000000
VERSION     DACEPW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.158"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..238)
                     /locus_tag="IW819_002211"
     CDS             complement(<1..238)
                     /locus_tag="IW819_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1245302.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTE"
     gene            573..1007
                     /locus_tag="IW819_002212"
     CDS             573..1007
                     /locus_tag="IW819_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1245303.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1030..1350
                     /locus_tag="IW819_002213"
     CDS             1030..1350
                     /locus_tag="IW819_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245304.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1491..1682
                     /locus_tag="IW819_002214"
     CDS             1491..1682
                     /locus_tag="IW819_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245305.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1753..2010)
                     /locus_tag="IW819_002215"
     CDS             complement(1753..2010)
                     /locus_tag="IW819_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1245306.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2168..2431
                     /locus_tag="IW819_002216"
     CDS             2168..2431
                     /locus_tag="IW819_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245307.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2455..2643
                     /locus_tag="IW819_002217"
     CDS             2455..2643
                     /locus_tag="IW819_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245308.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2726..2911)
                     /locus_tag="IW819_002218"
     CDS             complement(2726..2911)
                     /locus_tag="IW819_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245309.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3126..3497
                     /locus_tag="IW819_002219"
     CDS             3126..3497
                     /locus_tag="IW819_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1245310.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3647..3719)
                     /locus_tag="IW819_002220"
     tRNA            complement(3647..3719)
                     /locus_tag="IW819_002220"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3684..3686),aa:Gln,seq:ctg)
     gene            complement(3765..4193)
                     /locus_tag="IW819_002221"
     CDS             complement(3765..4193)
                     /locus_tag="IW819_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245311.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4253..5011)
                     /locus_tag="IW819_002222"
     CDS             complement(4253..5011)
                     /locus_tag="IW819_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1245312.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1778 a          790 c         1002 g         1546 t
ORIGIN      
        1 cttctgttgg gattgttaat tgttgtcctg agtagatcag gttaatatca gaaattgaat
       61 ttgattctgc aatggcattg atcaaggagt tatcaccaac gtatttttga gaaatcgttg
      121 atagagtatc acctgattga acggtataag cttcgtccgc atgagcagtc gttcccatga
      181 agaatgcagc gccggcagcc aaagttgttc caaaaagtaa agttttaagt gatgtcatga
      241 aaaatctcct ttattcgtta aaaagtttta tttgttgtgt tcaacgagga atactttaac
      301 acgtgaaaat atttcatagg taaaagaagc atgacaattt atgacaaatc cacgtttttt
      361 gtaacagaat gatgttcgct cgatgtgaaa aatgatgagg aatttcacat aatgcagaaa
      421 taatgggatt agaacaggtg ttctgatgag aaaagaaatg tataaataac ttgaaagaaa
      481 ttgcttttgt tcatcatttt atttacatat tgcgtatatt ttttgtattt ttgcttgaga
      541 tgttacaata aaagaaaaag gaggcggttc caatgagtaa aagataccag aacatcatgg
      601 tagcagttga cggatcaaga caatcgatca aagcgttcgc tgaagccctt gacttagcaa
      661 aggacaatga agcccactta tttatcgtgt caatcatcaa caaagttgag ctgacccata
      721 gtgcctatgc cttttcaaaa atctatgcag atgaaaaaca aagaacagaa gtagccatgc
      781 tgaaaaaaat caatgatgcc aaagagtacg gcattgctga gatccacgca atagttgaaa
      841 ctggagatcc aagaaatttg attgccaatg tcatccctgg acaagaaaat attgatttga
      901 tcgttatggg agcgacagga aaaggtgcta tacaacaagc attagttgga tcgaccgctt
      961 cttatgtcgt atcacatgcc ccttgcagtg tgttggtcgt gaagtaatga gataagggag
     1021 gaatagaaaa tggttcgtcc tcaagaagtc cgtgcgccaa aagaaaaaat agaaatttta
     1081 gcaattattg aagacggcac acaaacaaag aagggatact cgatcgcttt agtaaagtgg
     1141 aaaggaaaaa aaggtgttgc tctgagatgg gatggagata accaacaaga taaaggattc
     1201 cctgtcacag ctaacggtta tcatccagcg tggtttgttc tgcctgataa atttatggaa
     1261 ttatacagct atgattatgc aagagccgca gcatcaatca aagcattaga taaattaaca
     1321 gaagagcaga atgaaatgga ggaaaaataa aaaagacgaa ccaatcagcc tttagaaaac
     1381 tgattagttc gaggagtaaa aatgaaaaaa ggttatttgg gatgaaacta gaataatgcg
     1441 tataacttaa ataaaactta aagcaataaa atggcaagag ggtgaggcaa atggaagcag
     1501 gaaaagtatt acaggaagta aaagcactat gtgcagataa aaaatatgaa gaagcgaaaa
     1561 gcctgatcga aaagaacaaa gaaaatttgg aagaaaagta cccgatcgca caagaattta
     1621 tagaattaaa acaaggaacg atccttcacc gattcaaaca tttttttggt ataacagaat
     1681 agaaaaaacg attaaagatc ggcagaaatt tgctgcatct ttaatcgttt tctttttaaa
     1741 catgcgaggg attcaattct gattcttata taaaaaatag atccaaataa taagaaaaat
     1801 tactgaaagc gtcaattcga tgatgtgtgt tattaataca gacgttgttt cccaatgcct
     1861 aaaagaaaaa atttgataag taaaatatac agcaggtaaa gaggccagta aaactgaaaa
     1921 cttctttttt gtccatttta tgtaatagaa aagaatgctt atcataataa aaaaaggaat
     1981 ataaaaaaca aattgaccga tcgcgatcat actattgcct ccgataaata aagtccatga
     2041 tacaaaataa cactttgtgc gaaatggcgc aagcagtttt gtggatttat ccggtaaaat
     2101 ctgtatcgat tttgaaatta gaggagaaac ttgctataat caaagtgaaa gaaggagtgg
     2161 gattttcatg gatcatgtag tgttagaaat cgatttgcat ttgatcaata atattcgggt
     2221 gatttactat gttgtaagta acagtgtcga acaacgtgta ctgacgaata aaataaatgg
     2281 tattttagca aagaaagacg ttcaccgatt caataatggt gaaggaagtt attatagtat
     2341 cccagtagaa aaaattattt atacgacagt caaagtcaga gaagatctag ataccaaaaa
     2401 agcatatgaa cctattttta caacttattg attgacagca gatggaggaa aaaaatgaat
     2461 ccatatgaac aacttattca agatttgatc gatggaaaaa tcgaaaagat tgatgtaaaa
     2521 agagaagaat taatggcttt tcgagaagcg tggctgaagt tggagaatcg taaatatgtg
     2581 gtgggaaaag cctcactgaa cggcaatgtg acttatcatt acgatccgac acgtttattt
     2641 tagtatgaag tattcagaaa agaaccaaag gaggacgatg cagcagcatt gtcctccttt
     2701 gattttacaa aagtgaaagt tttacttatt tgaatagatt tttaaatttg tttttgacac
     2761 tgtcgatatt tacatctttt aaaagatttt gggctttctc tttataaggt cctaattgtt
     2821 ctttatgttc ttcgataaat cgttttgcgc catcgaaatt gccgttctta atcatttcct
     2881 tgactttttg aaataattct tcttgattca ttttgaacac atcctttgct atcaacatga
     2941 ttttttacat ggacaaaacg agaaaaactg atttatccac acgtcaataa tatcaaataa
     3001 cgatcatttt tactaaaaaa acactcgatt ctcgcttcta aagtaggaga aaaatctcca
     3061 acttgggacg gatggaattt ctttaactga cccatatact gaatatataa caaggaggta
     3121 gaaagatgaa agtgattaaa tatttgattg gactggcggc gattagtggt attttgctat
     3181 ttggactgaa atattatact gaaggaagtt atggagaaat ccctggtata cttgatcagc
     3241 taaatccatt agttgaaaaa ggagaggtct atgtaaagac acaaaaacca gaggaagtaa
     3301 atgaatatgg tacagcaagg tatgtgcaga aagctgctga tgccaatggg aaagaacgaa
     3361 cggttgaatt taatggtctt tctgtgttaa aagaaaatca ttatttgaaa attagcaata
     3421 aaggagcaca catagaaacg tatgaggaag ttcctagaga aaaagttccg gagaaagcat
     3481 tagcaattat tgggtaaatc agtttttgta ttaagaatac gcccgatttt attattcata
     3541 atttaattat gtaaactaaa tacaagtagt aaaaataata caataatccc cctgcaaaaa
     3601 gattgttctt ttacaggggg ttcatttttt cagcaagcac atcaaattgg gatggcaggg
     3661 ttcgaaccta caattgcttg attcagagtc aagtgcctta ccagtttggc tacatcccat
     3721 ttattcaaca aaataatcat agcactttcc taaaaaaacg caagttattt ttcttgatcg
     3781 gatagttgtt gctttactcg taaaaggcgg cgtactagtt tcttttctaa tccagcagga
     3841 agggcaatca gttctgctac tgacaagacg gtgtccaata actgatattc tttgacaggt
     3901 tcattcataa tatgatagat gtttgctaaa cgttcaatat attcaaagcg agtatactct
     3961 gttcgagcaa ttagaaattt caataaatcg gtggctttct cgtactcttg atctgcgtaa
     4021 taaacaagag attgacgata caatttgatt tccagttcat tgagggtagc aggtctgcga
     4081 cgctcgttcc actcattaat agagacttta ccttgaacat ttcccttgca gtcacggaca
     4141 aaaataatcg gaatttcatt tggatccatt tttttatgtg tgaatttttc cattatctac
     4201 gctcccttca aaggtttggt atcttgagta taagggattt ttgtttgaat tactagcttt
     4261 tctccaaagt tagcatctgt ccacccattg cagtgaaaat cgccgaaaaa tcgttgatcg
     4321 gtactgctcg attttttaca ccatagggat cgttgacata aatattttct ttgtcatatc
     4381 cagtgactac acaagcatga acaagaggag tgactcttac ttctccatta tttgtggtcc
     4441 aagtcataaa atcagtttca tctgggactt caaaattgat agtcacagaa gtccagacag
     4501 gatttccttt agatagtgct tttttgatat catcgagttg agcatcggtt tttggaaaag
     4561 tgtgataatc cgtttggacg atttgctgag caagggaggc aactggggga acatagacga
     4621 ccatagccca gtctccgcca ctgatatcac caacaaatcc ttcattagga tcgccatgag
     4681 tcccatctgc attgaaaaca gggacgtaat ccaattgctc agctaactgg tttttagttg
     4741 tttcataacc ataaaaagat aaaagcatag aaagcgaagt gacttcacag ccattttcta
     4801 aagaaggaga atcaaactgg ctctctaatg gaacatctag taaaacttct gctttctctt
     4861 ttgtcttttt cgttttttgt gttgaacttt tttgtgcagt gttcattgta tgttctggag
     4921 aatggatatg taggtaataa cttgtaccgc ttaataaaag agcagctgtg ccccctagaa
     4981 acaatagttt tttgaacgtt tttttcttca tttgaaatct cccttttcag ctgtttatct
     5041 ttcctattat aaatgtattt ttattggaaa tggaagtatt atattttaat atgtaggaaa
     5101 taattagttt ttaagg
//
LOCUS       DACEPW010000156         5067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEPW010000156 DACEPW010000000
VERSION     DACEPW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.159"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..747
                     /locus_tag="IW819_002223"
     CDS             1..747
                     /locus_tag="IW819_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1245313.1"
                     /translation="MALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAANPED
                     VVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAALQNGT
                     DSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNAVIRN
                     SATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGRQSVE
                     LFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(833..1573)
                     /locus_tag="IW819_002224"
     CDS             complement(833..1573)
                     /locus_tag="IW819_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1245314.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            1634..2206
                     /locus_tag="IW819_002225"
     CDS             1634..2206
                     /locus_tag="IW819_002225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245315.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFLCFFPLC"
     gene            2200..2886
                     /locus_tag="IW819_002226"
     CDS             2200..2886
                     /locus_tag="IW819_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1245316.1"
                     /translation="MLISFQVKERQHQESLQLYQINEEYYHHLEEFKHDYKSLLFSLK
                     ITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRGVFLKFLEKAESEKIP
                     IQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITINLLEEKEGLRFSIAN
                     KANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFFVEMY
                     LASRKSKSAY"
     gene            3187..3435
                     /locus_tag="IW819_002227"
                     /pseudo
     CDS             3187..3435
                     /locus_tag="IW819_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            3557..3883
                     /locus_tag="IW819_002228"
     CDS             3557..3883
                     /locus_tag="IW819_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1245317.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            3951..4664
                     /locus_tag="IW819_002229"
     CDS             3951..4664
                     /locus_tag="IW819_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1245318.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            4974..>5067
                     /locus_tag="IW819_002230"
     CDS             4974..>5067
                     /locus_tag="IW819_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245319.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAF"
BASE COUNT         1744 a          831 c          991 g         1501 t
ORIGIN      
        1 attgcattaa gtaccgttct agctggaagt ctattattat gtacacaaag tgtttttgct
       61 gagaataaga gctcagccac tacagaagga acgatttctg taacaaaacc agatgccgca
      121 aatcctgaag acgttgttcc agttcctcct gttgatccaa atgatcccga tacgccgttt
      181 acacctaacg aaccaaatca gggaacatcc ggattactga cgattgacca agctccagat
      241 cttgattttg gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc attacaaaac
      301 ggaacggaca gtacagggac agcaacacaa gtacagaatt tcgtacaagt aacggataaa
      361 agcggaattt atgccggttg gaaactatca gtcaaacgaa cagaatttat tggtacaaca
      421 tctgatacga ataaactaac tggttcaaag gtgaaattca aaaacgccgt gattcgaaat
      481 agcgcaacta atagcagtgg aacgccatct actatttctg gtactggcgc tgaaggaatt
      541 gagatcccag cagatcaggc agtagatcta gtaactgcag gagcaaatga agggattggc
      601 acatggattt attcattagg agagaatgtg gaacaaggga gacaatcagt ggaattattt
      661 gttcctgtat ccaattatgc cgtagacaac tacgtttcaa cattcacatg gtcgttaaca
      721 gatgctccta cagaagggaa agcgtaaaat caaatcaatg acttcaggga gtggagccga
      781 ttcaaggcca actccctgaa atctgctatg cttacgtctc agctcttatt catcaactcg
      841 aagcagtttt ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc tttcgaccaa
      901 cgagtaagga gtcaccattg aaaaattcta acacataatg agcagaatca attttctgca
      961 attgcgataa gttgatacag taagatttta atagtaaaaa gtaagcatac ttttccaact
     1021 ctgtcttcaa ttgttttaaa gaagtattca ctaatttatc ttctgtagtt gttttgacca
     1081 taacttggtt tttcaaacct ttgatacttt caatatataa aatttcccga ggatacagga
     1141 cgagacttcc acctttttga tcaataaaga ttttatcgac atttacgatt tgctgttctt
     1201 tccaattgat ctgttctagc acttgacgaa tttgttcaga taaattcctg tcttttctga
     1261 tatatccaat cggaaggatt ttccgattga tgatctctac tgcaaaattc gtataagcgg
     1321 tgaaaaaaac aatattacta tgtgcatttt ttttacgtat ttgttcggcg acatcaatac
     1381 ctgtgagtga ttgcttcaag tcgatatcga taaagaaaac atcgtgatta tcaatagtac
     1441 ccaactggat caactgattg atttgagaga tagaagtgag ctgcacgata ttctcgatat
     1501 gatgattgac caattctttg atgatccatt cgctgtgaaa ctggttatct tctatgataa
     1561 aatacttttt catatgcagg ctcctattaa aaaaatatgt gtacattttg aaacaaaaca
     1621 atggaggaat aagatggtcg caacagtgac actttatttg acctttactt tgattttaga
     1681 tttaggtatc tatttattct tttcgaaaca actgctagta aaaaacagct ttggttttat
     1741 agctttgatg gtggtgacac ttttatttcc tatccctaaa gtgagctttc cttacctagt
     1801 tggcttatta ttgatgtttc ttttttattt tactacgaaa aacctgttta tcagctgttt
     1861 gatcagtaat cttttttttg ctatcactgg actcagcttt tttctggttt ttgatctgat
     1921 ggattattta ggaaagagac atcttggaac agcaagtttt tttgcactac ttatcgcatt
     1981 tattttctat ctattagtaa aaagtataga caaacagacg agatttctca acttgctttt
     2041 tacttatcca actgaacact attttggagg tatttgtatt ttattaagct ggggcgtgat
     2101 ttacttggta ttgggaagtg ttcacttccg ctcatctaat tatctgcttc tatctctttt
     2161 gtcgatagtc atcagttttt ttctttgttt ttttccatta tgttgatcag tttccaagta
     2221 aaagaaagac aacatcagga atcgcttcaa ctgtatcaaa taaatgaaga atattaccat
     2281 catctagagg aattcaaaca tgattataaa agtctactgt tttcattaaa aattactctt
     2341 caggaaaata aaaaagaaac gttggaattt ttagaaatct tagaagatca ttctgcagct
     2401 atttttgaaa aaccgcaaat acaacagcta acaaatattg tatccccagc tgtcaggggt
     2461 gtctttttaa aatttctaga aaaagcagaa tcggagaaaa tcccgatcca agtagttatt
     2521 cctgtcgaag tgaaaacaat cccgattgat ctggttgttt ttattcgctg tttatctatt
     2581 tttatttcta acgcattcga tcatcgtgta gccgatcaga gtccgattac gatcaatctg
     2641 ctggaagaaa aagagggatt acgttttagt atagcgaaca aggcaaatcc gacgaatttg
     2701 acgctatcgg agatggtcaa aagaggaaaa acctctaaaa aaaatggcgg gttaggtcta
     2761 tacagtgcga aaaaaatgtt ggatgtttac gaaaatgcag aactaaaaat cgaattttca
     2821 aaacagaatc actatttttt tgtagaaatg tatctggcat ctagaaagag caaatcagcc
     2881 tactgactta ctgtcaaagg ataagcttga ggaaagtcta cacggagata gagacaacaa
     2941 cacagcatat tcaaaagaaa cagaagcgtg atgaccgttg tcacccttct gtttcttttg
     3001 ctatgtagtt aaacaaagta gttgacactt ccgctactta gtattagtac gaaagtatac
     3061 ttcatgatac taagtagata agaagtgaaa agcggaaccc acgatcgtca aaatgctaac
     3121 tgctttgctt acaccagata aagggaagag caatatcgct tattttaggc gtggagaagc
     3181 aagaaaatcc ctacgatcac acaaaaaatg ctgacccagc agttaagaga gttagaacaa
     3241 gatggcattg tccatcgaaa agtctataac caagtcccgc ctaaagtaga gtactcgctc
     3301 accgaagaag gaaaatcatt aagagagatt ctagtagcga tgtctgtttg gggagaagaa
     3361 cgtatcgaaa aacaaaaagc ctcaggggaa gaagtcacat tactgcatac acatggtggc
     3421 tacctaaatt actgatttct atgacattta ttttaaataa aagaaaacag gagttttttt
     3481 aaaagcatca tctatttttt atgtaaaaaa atggtatgat taatcaagta agaaatgatt
     3541 ggaagaaggc aagcaaatga atagcatgga aaaagttaac cagtttagag atgaacgtaa
     3601 ttggcgacaa tttcataacg aaaaagattt ggctatttct atttctttgg aagcatctga
     3661 attgttagaa ctttttcaat ggaaaacgcc ggaagaagta aaagaaacac aattagaacg
     3721 aatcaaagaa gaactggcgg atgtattgat ctattcatac atgatggcag acaaccttca
     3781 gctggatcta gatgagatca ttgaagaaaa attgatcaaa aataatttga aatatcctgt
     3841 agaaaaaagc gcaggtgtac gaaaaaaata cacagagttt tagaaatgtg tcattcggct
     3901 gttggaaaga gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa
     3961 aaggaaaagc aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta ttagtaggac
     4021 tagctttgat tttcaatgaa cagatcaagg attatcttgt cagagagaca ggagataaat
     4081 atgctatagc gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt
     4141 ttgactttga tgcggtagaa ccaatgacga cagaaggggt catgaggtcg cagatgagag
     4201 gaacagatct accagtgatt gctagtatcg cagtcccttc agtttcaatc aatttaccga
     4261 ttttcaaagg cttggataat acgtcactac tttacggagc aggaacatta tctccagatc
     4321 aagaaatggg aaaaggaaat tatgccttag caagccatcg tgcaacgaac cctgaattgt
     4381 tgtttacacc gctggaaaat ttagagatgg gtgccaagat ctatttgaca gatttagaaa
     4441 atgtgtatac atacaaaaca ttttttaaag aaaaagttgc tcctacagat acccaattac
     4501 tgaatgaagt cgaggaaaaa gaaattgtca cactaattac ttgtggcgac atggacgccg
     4561 tcacgcgact agttgtacaa ggagaactgg aaagtgttac accgataaaa gatgcgacag
     4621 atgatatgag atctgctttc aatctagaga caaaaacatt ttaggatact gggacataag
     4681 tcgttacaga ttaaaaacag ccaaaggata tgaatcgata aggaaaaacc aatttgctga
     4741 aaaaaagagg aacgcaaaga ggtgcatctc tgatttggtt gttttctaga tagacttgtg
     4801 ttccagtttt tttatttttt atatgaaaag gcttgcataa ataaaaattc gatggtatga
     4861 tgattttgaa taagaaatgg attgttactg tacggcaggc aaaaccagaa ttcagctaag
     4921 aaacagtagg cgaattctag gtttttttat ttttcttgag agggcgaagc aaaatgaaaa
     4981 ttgataaaat tttgaataac aatgtcgtca tttcgaaaaa tggattcggg gaagaagtcg
     5041 tttgtatggg aaaaggacta gcttttc
//
LOCUS       DACEPW010000157         5059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEPW010000157 DACEPW010000000
VERSION     DACEPW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.160"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..141)
                     /locus_tag="IW819_002231"
     CDS             complement(<1..141)
                     /locus_tag="IW819_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1245320.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGS"
     gene            334..1134
                     /locus_tag="IW819_002232"
     CDS             334..1134
                     /locus_tag="IW819_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1245321.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1153..3021
                     /locus_tag="IW819_002233"
     CDS             1153..3021
                     /locus_tag="IW819_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1245322.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3014..3505
                     /locus_tag="IW819_002234"
     CDS             3014..3505
                     /locus_tag="IW819_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1245323.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3493..4047
                     /gene="srlA"
                     /locus_tag="IW819_002235"
     CDS             3493..4047
                     /gene="srlA"
                     /locus_tag="IW819_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1245324.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4065..>5059
                     /locus_tag="IW819_002236"
     CDS             4065..>5059
                     /locus_tag="IW819_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1245325.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1769 a          805 c         1047 g         1438 t
ORIGIN      
        1 tgaaccacca gcaatcatag ctactaaatc aagttcgaac aagcctacca acagccagtt
       61 caaccctccc acaatcaata aagttaatgc aatagcatct aagttttcat tagaattctc
      121 ctttcttaaa tgataaaaca attatatatc aaaaagagaa acattgctta tataacgctc
      181 aggaatttta gcaatctatg caaaaagatg aaaatttttt ttgctcttcc attagtacac
      241 attttttagc tgaagctgta aatttcatgt cttaattttt tttagtgagc ttttatactg
      301 gatttgtaag aaaaaagaag gagaggatca catatgtcgg attggttgga tattgcagga
      361 aaagtagtaa ttgtaactgg aggttcctca ggaattggaa gaagtatcgt cgaaaatttg
      421 ttgaagcaga atgcacaagt agcgaatttt gatgtaactg aatgccgtat acagcatgaa
      481 aacctactat ctttaaaagt cgatgtgtct tctaaaacgg acatagaaga agggatatat
      541 aaagtaatga aacatttcaa gacgatcgat ggtctagtca ataatgcagg aataaatatt
      601 ccttctcttc taatagatag aaatcatcca aaaagcaagt atgaattatc agaacaagtt
      661 tttgataaga tgatcgctgt caatcaaaaa agtgtatatt tgatgagcca agcagttgga
      721 agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc tttcttctga atcaggattg
      781 gaaggatcag aaggacagag ctgttatgct gctacaaaag cagcgatgaa cagttttact
      841 cgttcttggg caaaagagtt aggaaagagg aatgttcgtg tggtaggtgt tgcaccaggg
      901 atactggaag aaaccggttt gagaacacaa gaatatgaag aagctctttc ttatacgaga
      961 ggtataagcg tcgaacagct gagaaacggt tacagtcgaa caagtacgat ccctctagga
     1021 aggagtggta agctgcaaga agtggcggat ctagtctgct attatctttc agaaagatca
     1081 agttatatca ctggagtcac gactaatatt tcaggaggta aaacaagagg ataaaggata
     1141 ggggggacgc acgtgacgct agtaaacaga tggtaccaat tattgaaatt actagtcact
     1201 cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca aaaccagtaa tcaaaccata
     1261 aaaaaagata ttggggagtt gaatgaagaa attgccggta ttgcaaaaat catccaaaaa
     1321 aacaaacatt ttgaacttga aatttatgat tttgattttt ttgatgagat catgcaagga
     1381 aaattgaaaa cagccagtga tttcaattcg gcatccaaaa gaatcgctta tttactaaat
     1441 aggctgatcg aaacagagga tttcttacgt atggatgatt tgtctgaaga agtcggtgtc
     1501 agcagaggaa cagtggtcaa ggatctaaag acattaaaag agatgatcaa tgattttgat
     1561 gtaaagatta caggcacacc aaataaagga ctaagaataa taggagatga gctagaatta
     1621 cgactacttt tattttattt tgtttctccc tacttttcag agacgaaaac agaacagttt
     1681 ctagaagaaa aacaagcaat caaacatttt cagaagaaga cgaatgctag taaacaagaa
     1741 atcacccttt taacgaaagt tatcggtatt tctttgaata gaatctctag taactatctg
     1801 ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat atatccaaga attcaatgaa
     1861 ctgatcaatc agttggaaga aatctatgaa ctaactttga gccaatatga aaaagatttt
     1921 atcagttttc ctttgaacat catcaacaca agtataacga tgataaggaa agctaactct
     1981 gatttgcgct tatatttcga ccccatgatg atgcgtattc gacgattaga tctcattgat
     2041 ctggatgagt ccttcttata tcaagagatg aaacagcacc tctctttatt gataaatcgg
     2101 gtaatttttc gttatcgatt gactgattta ttttatggtg agatagaaaa aaagtatcct
     2161 ttttcttacc aaatcgctag cgaatgcata caagcattag aagctatctt gcaacataag
     2221 atctctaaag tagagatcag ctacctaacg ttgtatttgg agttagcatt tcgatatcaa
     2281 aaagagcatt tttcctctaa aaaagtagcc attgttcata cgacaggaaa aggaacagca
     2341 ttaatgatcc aaagacaaat caaaaggata ttggggaggg atatacaaat aacacatgtt
     2401 tcagaagaac aatatgaaaa agtagatcta aacaaatatt tcgctgtttt tacaacgatt
     2461 ccgttaaaaa atgtaaatga agagacaccg gtgatccatt tgactaatct ttttgatgac
     2521 tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc gattagccgg gtttcattct
     2581 actgtttttt tctatcaaat atggaaagaa aaaaagacgt atcaagaata tttaaacaaa
     2641 ttagtcgctc ctttgatcag aggctattat gtggatgaag aatttgaaaa aaggatcttg
     2701 aaaagagaat atcagcaaag cacatttgtt aacaaagaat ttgcttttcc tcacacaacc
     2761 aaccagctat ctcaacacat tgttttatca gtagggctaa taactgatca aggtttagca
     2821 gaaacaggag gaaatggagt tattttttta tttgccatac ctgaagaaat gacggctgaa
     2881 gttgagaatg aattatttga gctatacgat atcctatttg aactcataaa aatgggaata
     2941 gatgagttta ttgaattagc accgacacaa gaatcatttt taaaactaat caaaagtaaa
     3001 ggagagagac ttcatgaatg atatgtatct attcatagtt ttcgcagctt cagcttatgt
     3061 gctgcaagca gttatcagtt ggaagcaact aaagcatttc aatcaaacat atagcgaact
     3121 gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg aaaatccgtt caggcacgat
     3181 tgttatgcta gctatagacg atcatgatca agttttagaa gtcagaaaaa tgcaaggggt
     3241 tacatctttg gcaaaatttc attcattacc tgtctatgcg ggtgaagata tccattacat
     3301 cgatcgttac catccctcag ttcgagcgga agatcgtttg acacaagcag ctttagaaaa
     3361 tgccagagaa gtatacttac gaatagaggc aggaaatgaa aaaagtgaac aaccttcatt
     3421 gccttttatt gctatgctca ataattttcg gatatggaaa agtacgatca aagaaaaaag
     3481 gagtgacaaa cgatggatat cataacaaaa tttgctgagg gatttatgag gctgttccaa
     3541 acaggtgcag aaacattcat aagctggatg acaggaatcg taccagttgt cttgatgctg
     3601 atggttgcaa tgaatacgct gattgctttt ttaggtgaag accgagtaac gaaagtagcc
     3661 aaattatcag cgaaaaatcc gcttactcgt taccttgtgt taccgttctt atctgctttt
     3721 atgttgggaa atcctatgtc atttacaatg gctcgtttcc ttccagaata ctacaaacca
     3781 agctattatg ctgctcaagc tcaattttgt catacgagca atggtgtatt cccccatatt
     3841 aatcctggag aattgtttgt atggatgggg attgctcaag ggatcgaaac cttagggcta
     3901 aattcgatgg agctggctat ccgatacttg cttgtgggac tgctgatgaa ctttattggt
     3961 ggttggataa ctgattttac tactggattt gtatgcagac agcaaggaat cgttcttagc
     4021 aaaaaagtag agctttcagt tgattagaga aaggagagtt ttaaatgtat ggtgatatta
     4081 agatcgtaag aggaaacggc gggtatggag gtcctttgat tattaggccg acaaaagata
     4141 aacacaagtt catctatgtc acaggtggag gtgagaggcc aatgatcgta gataaaatag
     4201 aagaactgac aggtatggag tctatcaatg ggttcaaaac atcaatacct gatagtgaaa
     4261 tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg tggaatttat cctaaaaaag
     4321 ggataccaac catcaatatc gttcccacag gaaaaagtgg cccattagca caatatatca
     4381 ctgaagatat ttatgtttca gcagtagggt tagaacaaat acagccacta atggaaactg
     4441 atgataacaa agaaacagta aatactgcat ctccagataa aaagtacaaa tatgatactt
     4501 ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg gatagcaaaa attggaatgg
     4561 gcgcgggaaa agtagttgct acattcaatc aatcagcaag agaagcagta gatacgatga
     4621 tccggacagt gatccctttt atggctttcg tttccctttt gattggtgtg atacaaggtt
     4681 ctgggatcgg taatgtattt gctaacatta tggcaccttt agcaggaaat gcagttggat
     4741 taacaatcat tggctttatt tgttcactgc catttttatc tcctttgctt ggtccagggg
     4801 cagttattag ccaagtgatt ggaacattga tcggtgtaga gattgggaaa ggaaatattc
     4861 ctccccaatt agcattaccg gcactatttg ctatcaatac tcaaaatgca tgtgatttta
     4921 ttccagtcgg tctaggtttg gaagaagcaa aagctgagac agtagaagta ggtgtaccat
     4981 cagttcttta ttcaagattt cttaacggca tcccacgagt tatcattgct tgggtagcaa
     5041 gttttggatt atatagctg
//
LOCUS       DACEPW010000158         5051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEPW010000158 DACEPW010000000
VERSION     DACEPW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.161"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2139)
                     /locus_tag="IW819_002237"
     CDS             complement(<1..2139)
                     /locus_tag="IW819_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ1245326.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIY"
     gene            2285..2860
                     /locus_tag="IW819_002238"
     CDS             2285..2860
                     /locus_tag="IW819_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1245327.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3074..3769
                     /gene="vanR"
                     /locus_tag="IW819_002239"
     CDS             3074..3769
                     /gene="vanR"
                     /locus_tag="IW819_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1245328.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3747..4901
                     /locus_tag="IW819_002240"
     CDS             3747..4901
                     /locus_tag="IW819_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1245329.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1582 a          944 c          984 g         1541 t
ORIGIN      
        1 gtagatggtg gctccttttc cagttccata atgtggattt gcatctgcat gtagtgatga
       61 aacacctagc tgcattctca taccatctga cgaagatgtt gtaccgtcgc cccaatagaa
      121 aggcaattgt aatttatgat gaaagtttac taatatggct tgggctttat tcatggcatc
      181 ttcatacatg cgccattgag atacattggc tagttgctta tatgtaagtc cgggtgtggc
      241 ttcggccatc ttgctcaagc caatattcat tcccattcct aaaagggcag ccatgataat
      301 gattgtttct tccttatctg gttttcgatt attggaagca tgagtgaatt gctcatgaaa
      361 tcctgttata tgggccacat ccatgagtaa atcagttaat tttattcttg gtagcatctg
      421 ataaaggctt gcactaaatt tttttgcttc ttctggaaca tctttttcta agcgtgcaag
      481 tgatagcttt cctttttcaa gagaaacccc atctaactta ttggaattgg cagctaacca
      541 ctttaacctt tcattaaagc tgctggttct ctccgttata taatcttcga atgataaact
      601 aactgataat ctcgtattcc ccttcgattg attccatgta tcttccgaaa acaaatattc
      661 ctcaaaatcc ctatattgtc tgctgccaac aatggaaaca tctcctgccc gaacatgctc
      721 ccgaagttct gttaaaacag ccatttcata gtaatgacga ttaattgttg taccatcatc
      781 ctcgtataaa tgtcttttcc atcgttttga aataaaatcc acaggtgagt catcaggcac
      841 ttttcgcttt ccagattcgt tcattcctcg gataatctca acagcttgta aaagtggctc
      901 atttgccttt gtagaatgaa attccaatac tcttaatagc gttggcgtat attttcttag
      961 tgaataaaac cgtttttgca gtaagtctaa ataatcatag tcggcaggac gtgcaagttc
     1021 ctgagcctct tctactgaag agacaaaggt attccattca ataaccgatt ctaaaacctt
     1081 aaaaacgtct aatttttcct ctcttgcttt aattaatgct tgtccgatgt tcgtaaagtg
     1141 tataactttc tcatttagct ttttaccgtt ttgtttctgg atttcctctt gagccttacg
     1201 accttttgat aacaaactaa gtatttgcct atcatgaatt tcaaacgctt tatccgttag
     1261 ctcctgagta agttgtaata aatagatggt taatatcgaa taacgtttat tttcttgaaa
     1321 gtcacggaat gcatacggct cgtatcttga gcctaagcga gacagctgca acaggcggtt
     1381 acggtgcaaa tgactaattt gcactgtttc taaatccatt cctcgtatgt attcgagtcg
     1441 ttctattatt tttagaaaag tttcgggtga aggatgaccc ggtggctctt ttaaccaacc
     1501 caatatcgtt ttattggatt cggatggatg ctgcgaggta ataatccctt caagcttttc
     1561 tttttgctca tttgttagag atttactaac cgtattaaat agcttctttt cagccattgc
     1621 ccttgcttcc cacaccattc tttcaagtgt agtgatagca ggcagtataa ttttgttttt
     1681 tcttagaaaa tctatgcatt catgcagtag atgaatggca tcaccatttt ccaaagctaa
     1741 ttgatgaagg tacttaaatg tcattcgata ttcactcagg gtaaaagtta caaagtcgta
     1801 ttcacttcga atttctttca aatgatccca aagtgtattt tccctttgag gataatgatc
     1861 aagcgaggat ggactaacac caatctgttt cgatatatat tgtatgaccg aatctgggat
     1921 gcttttgata tgagtgtatg gccaaccggg ataccgaaga acagctaatt gaacagcaaa
     1981 tcctaaacgg ttttcttccc tccttcgctt attaactatt tctaaatccc gtttggaaaa
     2041 agtgaagtag gtccccagta tccattcatc ttcagggatt tgcataaaag cctgtctctg
     2101 ttccggtgta agcaattctc tacctctcgc aattttcatt cagtatcatt ccatttctgt
     2161 attttcaatt tattagttca attatatatc aatagagtgt actctattga tacaaatgta
     2221 gtagactgat aaaatcatag ttaagagcgt ctcataagac ttgtctcaaa aatgaggtga
     2281 tattttgcgg aaaatcggtt atattcgtgt cagttcgact aaccagaatc cttcaagaca
     2341 atttcagcag ttgaacgaga tcggaatgga tattatatat gaagagaaag tttcaggagc
     2401 aacaaaggat cgcgagcaac ttcaaaaagt gttagacgat ttacaggaag atgacatcat
     2461 ttatgttaca gacttaactc gaatcactcg tagtacacaa gatctatttg aattaatcga
     2521 taacatacga gataaaaagg caagtttaaa atcactaaaa gatacatggc ttgatttatc
     2581 agaagataat ccatacagcc aattcttaat tactgtaatg gctggtgtta accaattaga
     2641 gcgagatctt attcggatga gacaacgtga agggattgaa ttggctaaga aagaaggaaa
     2701 gtttaaaggt cgattaaaga agtatcataa aaatcacgca ggaatgaatt atgcggtaaa
     2761 gctatataaa gaaggaaata tgactgtaaa tcaaatttgt gaaattacta atgtatctag
     2821 ggcttcatta tacaggaaat tatcagaagt gaataattag ccattctgta ttccgctaat
     2881 gggcaatatt tttaaagaag aaaaggaaac tataaaatat taacagcctc ctagcgatgc
     2941 cgaaaagccc tttgataaaa aaagaatcat catcttaaga aattcttagt catttattat
     3001 gtaaatgctt ataaattcgg ccctataatc tgataaatta ttaagggcaa acttatgtga
     3061 aagggtgata actatgagcg ataaaatact tattgtggat gatgaacatg aaattgccga
     3121 tttggttgaa ttatacttaa aaaacgagaa ttatacggtt ttcaaatact ataccgccaa
     3181 agaagcattg gaatgtatag acaagtctga gattgacctt gccatattgg acatcatgct
     3241 tcccggcaca agcggcctta ctatctgtca aaaaataagg gacaagcaca cctatccgat
     3301 tatcatgctg accgggaaag atacagaggt agataaaatt acagggttaa caatcggcgc
     3361 ggatgattat ataacgaagc cctttcgccc actggagtta attgctcggg taaaggccca
     3421 gttgcgccga tacaaaaaat tcagtggagt aaaggagcag aacgaaaatg ttatcgtcca
     3481 ctccggcctt gtcattaatg ttaacaccca tgagtgttat ctgaacgaga agcagttatc
     3541 ccttactccc accgagtttt caatactgcg aatcctctgt gaaaacaagg ggaatgtggt
     3601 tagctccgag ctgctatttc atgagatatg gggcgacgaa tatttcagca agagcaacaa
     3661 caccatcacc gtgcatatcc ggcatttgcg cgaaaaaatg aacgacacca ttgataatcc
     3721 gaaatatata aaaacggtat ggggggttgg ttataaaatt gaaaaataaa aaaaacgact
     3781 attccaaact agaacgaaaa ctttacatgt atatcgttgc aattgttgtg gtagcaattg
     3841 tattcgtgtt gtatattcgt tcaatgatcc gagggaaact tggggattgg atcttaagta
     3901 ttttggaaaa caaatatgac ttaaatcacc tggacgcgat gaaattatat caatattcca
     3961 tacggaacaa tatagatatc tttatttatg tggcgattgt cattagtatt cttattctat
     4021 gtcgcgtcat gctttcaaaa ttcgcaaaat actttgacga gataaatacc ggcattgatg
     4081 tacttattca gaacgaagat aaacaaattg agctttctgc ggaaatggat gttatggaac
     4141 aaaagctcaa cacattaaaa cggactctgg aaaagcgaga gcaggatgca aagctggccg
     4201 aacaaagaaa aaatgacgtt gttatgtact tggcgcacga tattaaaacg ccccttacat
     4261 ccattatcgg ttatttgagc ctgcttgacg aggctccaga catgccggta gatcaaaagg
     4321 caaagtatgt gcatatcacg ttggacaaag cgtatcgact cgaacagcta atcgacgagt
     4381 tttttgagat tacacggtat aacctacaaa cgataacgct aacaaaaacg cacatagacc
     4441 tatactatat gctggtgcag atgaccgatg aattttatcc tcagctttcc gcacatggaa
     4501 aacaggcggt tattcacgcc cccgaggatc tgaccgtgtc cggcgaccct gataaactcg
     4561 cgagagtctt taacaacatt ttgaaaaacg ccgctgcata cagtgaggat aacagcatca
     4621 ttgacattac cgcgggcctc tccggggatg tggtgtcaat cgaattcaag aacactggaa
     4681 gcatcccaaa agataagcta gctgccatat ttgaaaagtt ctataggctg gacaatgctc
     4741 gttcttccga tacgggtggc gcgggacttg gattggcgat tgcaaaagaa attattgttc
     4801 agcatggagg gcagatttac gcggaaagca atgataacta tacgacgttt agggtagagc
     4861 ttccagcgat gccagacttg gttgataaaa ggaggtccta agagatgtat ataattttgg
     4921 ctctttgtca aatgatgttg gtaaagagaa atcttagaat aattggagat caagtggatc
     4981 agttcctctt gatctttttt agttgaaaat caatgcgttg ccatgacgaa tcatgatgcg
     5041 caatttgaaa t
//
LOCUS       DACEPW010000159         5028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEPW010000159 DACEPW010000000
VERSION     DACEPW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.162"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IW819_002241"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IW819_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1245330.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="IW819_002242"
     CDS             complement(323..892)
                     /locus_tag="IW819_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1245331.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="IW819_002243"
     CDS             1078..1980
                     /locus_tag="IW819_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1245332.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IW819_002244"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IW819_002244"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1245333.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IW819_002245"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IW819_002245"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1245334.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
BASE COUNT         1435 a         1094 c          867 g         1632 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgc
//
LOCUS       DACEPW010000160         5026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEPW010000160 DACEPW010000000
VERSION     DACEPW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.163"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..124)
                     /locus_tag="IW819_002246"
     CDS             complement(<1..124)
                     /locus_tag="IW819_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAQ1245335.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAE"
     gene            complement(488..3154)
                     /locus_tag="IW819_002247"
     CDS             complement(488..3154)
                     /locus_tag="IW819_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1245336.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            3495..4946
                     /gene="cls"
                     /locus_tag="IW819_002248"
     CDS             3495..4946
                     /gene="cls"
                     /locus_tag="IW819_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1245337.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1462 a         1068 c          889 g         1607 t
ORIGIN      
        1 tttcagcagg caggactttc tccgttttca gataattttt tacatcttca gcaatagtta
       61 cttgcgattc ttgctggatt tcataatatt cttcagcaaa ttcatcccat tctttttcat
      121 tcatttgatt tcctctaatt ttttgttata tgttttcact tttcggaata aatcattctc
      181 ctcttttatc tattctatct cttttgatga ataataaaat gattggcttt ccgtctatca
      241 taaataaaaa aagggactgt gaccaaagtc gtgtcaaagt ccctctttcc gaataaacag
      301 tgtccaatca gatgaccctc agtattagct caaaatgctg attttacaac ttcttcctta
      361 aacggtgttc tcctgccaac tcttcggtca caagtcacaa tattcaacaa acatgagaag
      421 ctgtttgttg aaattgcacc ttgtgactca gaattaaacg gcaggctcac aacctcgttg
      481 attcgtttta tttatttcct tgtactcgta caatgatcaa tttagtgatt tccatcaaaa
      541 caattgtcac aagtgcgatt cccatacatg taaagacttc tgtcaatcca aatgctgatg
      601 ggatagagaa gacttctttc agtcctggga tcattgttaa gcagtaaagc gcgctacata
      661 caactacagc tagtaagaca cttctgttag agaagaaccc tgcttggata gctgtttgtg
      721 tgtttgaacg tgctgggaat gtttgcaatg tccgacacca aatcaatgtt gaaaatgaca
      781 tggctacacc gaattcaggt gaatgctgca tgccaagcca ttgagcaaag ataacagcaa
      841 tcccaatcag cacaccacga tacgttacag aaatcaatgt tttgtccgta aagattcctg
      901 aatttggatc tcttggcggg cggttcatcg tagcaggttc agctttttct gtacctaatg
      961 caattgctgg cagtgaatca ttgactaaat tgatgaacaa cagttgtaat gctgtaaatg
     1021 gattggacca gtcagcaact aaggcaaaga tgatcgcaat gatcgcacct aaatttcctg
     1081 caaatagata agaaatagct ttcttgatat tatcaaacac attacgtcct acttctactg
     1141 cgctgacaat agaagcaaag ttatcatccg tcaataccat cgctgctgca tcttttgcca
     1201 catctgtacc cgttcccata gcaattccga tgtctgcttg tttcaatgct ggtgcgtcat
     1261 ttacaccatc acctgtcata gctgagatat gacctttatt ttgccaagca cgaacgatac
     1321 gaattttatt ctcaggtgaa acacgtgcat aaacagtgac tttttctaaa acttcgttta
     1381 gttgatcttc tgtcaattca tctagttccg tcccggtcaa agccaggttt ccttcttcaa
     1441 aaatcccgat ttgttttgcg atagcaacag ctgttgtttt gtggtcacca gtaatcatga
     1501 ttgttttgat tcctgcactt cttgcttctg ttaccgcctg agtcacttca gcacgtggag
     1561 gatcgatcat agcaagcaaa ccaacaagga tcaaatcgtc ttcatcttca aatgataaat
     1621 caaaattatc aagtggacgg taagcaaaag ctagtacacg taatgcgcgt gttgaaaaag
     1681 cttcattttg ctcttggaat tttttcatca attcatccgt catcggaacg acttcaccat
     1741 ttagcaatac ttttttactg cgttggaaga cgatatctgg tccgccttta acaaacaagt
     1801 aatttttgtt atcaattgtg tgtccagtag acattaattt tcgatctgag tcaaaaggaa
     1861 gttctgcttg tcttgggtag ttgtttcgga tttcctgata aggctggttt aatttttcac
     1921 taaagtcgat aaacgctact tccgtagggt cgcccaattt agtaccgtct tcactgatcg
     1981 atgcatcatt cgctaaaaca ctgatttcga tcaagcgacg ttcgtcagct gtccattttg
     2041 aagggtcatt tgtgaaatcc ccagtatcgc cattacctaa aaagtagtca acaactgtca
     2101 ttttgttttg tgtcaatgta ccagtcttat ctgtacagat gatacttgtc gaaccaagtg
     2161 tttcaactgc tggaagcttc cgaataatgg catgacgttt tgccatttta ttcgttccca
     2221 aagataacac gattgttacg atagactgta atgcttctgg aatagcagct acggctacgg
     2281 ctacggcaaa catcactgcg ctgacgatat ctgcagtcat atctcctgta ccttctccaa
     2341 gataaatacg aaacagttgg atacctaaaa tcaataaaga caatgccaaa atagccaaac
     2401 ttagtttttt actgaattta tccagacctc gttgtaacgg agtgacttgc tcagttgttg
     2461 attccaaaag gccagcaact tgtccgatct ccgtattatt cccagttgaa gtcactaaga
     2521 atttaccgcg tccgtaagtt acgatcgttc cactgaatac catatttgta cgatctccga
     2581 ttggtaccgt acttgtgata tccgttactt ctttttctgc agaagtcgac tctcctgtca
     2641 gcataccttc atctactttt aatgatcccg catctagtaa acgtccatca gcaggtacat
     2701 aatcgcctgc gtctaacaac acgatatctc ctaccacaag ttcgtttgcc ggaatcgttt
     2761 tttccttacc atcacgaatc actcgagcat cgggcgctga aaggtttttc aacgcttcta
     2821 aagaaccttc tgcttttttt gtttggacaa cactgattat agaattcaac atcaatactg
     2881 caaaaataac taagaattcg atgtagtcac ccatgaccat ctgaatcagt gcgacaatca
     2941 gcaatacgat aaccattgca tctttgaaag tttctaagaa caatttccaa gttggcttct
     3001 tcggcttttc ttttagctgg ttcaagccat ctctttctaa tttttccttc gcttgcgtgg
     3061 tgctcaatcc atcggttgag ctctgagttt gctgcatcaa cgagtcgatc gtttctttat
     3121 agaccgcttg ttgtgcttgt tccttgtttt ccaaatttca tcccccattt tttgatcttg
     3181 gccctagtcc tgtctgaaaa aaaagagacc taagacaaat acgcatttgt cttaggtctc
     3241 accatttcat acaataccag aaaaccattt ggttaactta ctgttgatat tatcgcaaca
     3301 aacgttgccg gttactcccc taaggagata ttcaactgta ctaatatcct accttattaa
     3361 cctacaaaaa gcaactactt tagcccgatt tacctacatt ttcatgaaag gagtgaaaag
     3421 tatattcacc ttggtaggct tctttcttct atcatgctat actaatgtta ttgtatgtaa
     3481 ggaggaacat ttttgtggta tctagtatta taaccgccct ttatctatta aatgcactta
     3541 ttgctttggt ggctattttg atcaaacccc gagatgtagc agccatttgg gcatggctct
     3601 tagtattgtt tgcccttcct ggggtgggct ttgtattata tttatttttc ggacgtggat
     3661 taacggataa gaaaaaattc tatctccgac aaagtgactt gaaagaatta gaaaactttc
     3721 agtcttttag aggagatacc attgaacatt acgatcctga catgggcgat aaagacaaac
     3781 agcaatttgt tgacttcttc tcttcattaa atcgtatgcc gctgacaaga atgaattctg
     3841 tcactcttct cacagacgga caagagaaat tggattcact gcttcaagat ctaaaaaaag
     3901 ccaaacattc gatccatatc gaatattacg catttgtgac agataatatc ggccagcaag
     3961 tcttacatgt tttagaagaa aaagccgcag aaggcgtgga agttcgaata ttatatgatg
     4021 catttggctc tcatggcaca aaagcaaaag atttcaatcg tctaatcaaa aatggtggac
     4081 atgtccatac atttgttacc tcacaaaggg cattacttcg tttccgattg aattaccatg
     4141 atcaccgaaa aatcgttgtg atcgatggaa agattagtta taccggtggt ttcaatattg
     4201 ccaatcaata tgtaaataca acaaaaaaat tcggctattg gcgcgatacg catatacgga
     4261 ttttcggtgc cgcttcttct ttgctccagc ttcgcttctt aacagactgg aacgtctcgg
     4321 tacctgaaga aaaaaaggtc ggctatcatt tgaattattt ctttaaaaaa gcagatcgag
     4381 atgaatctaa gcttgctgat acatccatcc agcttgtttc aagcggaccg aataacgaaa
     4441 gggaacaaat caagctttca tttatcaaat tgattacttc tgctaaaaaa cgtgtttgga
     4501 tacagacacc ttaccttgtt cctgatgaaa gtgtcattgc tgctttaaaa atcgcaactg
     4561 cctctggtgt agatgtgaaa atcatgattc ccaacaaacc ggatcatcct tttatttatc
     4621 gagcaacaca atattatgct cggcagctga tcaaggaaaa tgtacaaatc cttgtctatg
     4681 agaacggctt cctccatgca aaaacattga taatggatga tgaaatctgc atggtaggtt
     4741 cagcaaatca agatattcgt agctaccgat tgaattttga aacaagtgct gtcatttacg
     4801 atcctgagtt tttagaagaa cttgctactc agttcaaaga agatgagaca cattgttcat
     4861 ccatgacaac tgaaacagtc aaggaaatgt ctaactggct attattcaag caacaaattt
     4921 ctcgattatt ttctccaatc ctataatttt tttattcaag tagagaaaaa tatactaaga
     4981 ttaaagggac tgtgacaaaa atcttgtcac agtcccttct tccgaa
//
LOCUS       DACEPW010000161         4951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEPW010000161 DACEPW010000000
VERSION     DACEPW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.165"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(51..824)
                     /locus_tag="IW819_002249"
     CDS             complement(51..824)
                     /locus_tag="IW819_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1245338.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            968..1318
                     /locus_tag="IW819_002250"
     CDS             968..1318
                     /locus_tag="IW819_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1245339.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1299..2015
                     /locus_tag="IW819_002251"
     CDS             1299..2015
                     /locus_tag="IW819_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1245340.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2015..3463
                     /locus_tag="IW819_002252"
     CDS             2015..3463
                     /locus_tag="IW819_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1245341.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3533..4042)
                     /locus_tag="IW819_002253"
     CDS             complement(3533..4042)
                     /locus_tag="IW819_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245342.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4032..4757)
                     /locus_tag="IW819_002254"
     CDS             complement(4032..4757)
                     /locus_tag="IW819_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245343.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1702 a          843 c          996 g         1410 t
ORIGIN      
        1 gaaaaaataa gcgattttct taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga
       61 tcaaaatact tcaatgcgtg gacaattccg ccttctgtat ttttctttgt gataaaggta
      121 gaaagttctt ttaatccgtc ataggcgttg cccatagcga ttttgttgtc gcaagcttcc
      181 aacaaggcaa aatcattcgg accatcgcca aatccaaaag taggaacttt ttccagctga
      241 agttcttttt gcagacgttt gacacctgcc ccttttgaga ttcctttctt cactgtatcg
      301 atcgagtaag gaccattccg ataaaaagtt agctcaggaa aatgttcgtg atagtattcg
      361 tcacctgatt gagataagat cagcaacata tttactgtgt tttctttaaa gcctaaagga
      421 tcaatttccg gaacatctga atgaatgaag tcgtaagcat tgatcattgt ttggctatga
      481 ccggtacacc agattttctg atcgttatag taagaaagtt catgaccgtt caactgtaca
      541 tgatcataca ttttttgaca ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg
      601 tctactcgaa tgaaagaacc attcatggtg accgcagaag taatgcccgc atcttccatg
      661 atagagtgga tttctgcttc tgtacgtcct gtagcgatga cggggagaac attgttttct
      721 tttaatgcgt tcatagcttt tgccacttcg ggtgtgattt ttgatttcgc atctagtaat
      781 gttccatcaa gatcaaagaa agtgattgca cgataatttg acacaaataa aactcctttt
      841 ttattggaat aagtttatct taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat
      901 tcctgaacat attggttcag tcaacaattt agagtatact ataagaagag aatttaggag
      961 ggaaagaatg atttttttca gtattggtat cttattctta ataattggca gtctttttcg
     1021 gattctgccc tcaaaaggaa atctgccttt ttatgggtat cattcgcctc ttgccgcaaa
     1081 aacagacgct cattggcggc tggctcaaaa gacaagcgga aactggtttt ttcttatggg
     1141 cttactgatg gcattgatcg gctattattt aaagacatct gggcatacga attattttct
     1201 gattgaaatg cttctgctag tatttccaat tatgcctatt tttattatga cagaaaagaa
     1261 attacagaaa tatgacctgg aaactggagg gaacgataat gaatatttta atgattgaag
     1321 ataatcaatc tgtttctgaa atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa
     1381 ctttcaagta tgatggaaaa gaagggctcg atgccttttt agaaaaccca caacattggg
     1441 atatgattac actagacctg aatttgccca cactggacgg tatgctagta ggaagagaga
     1501 tccgcaaagt ttcaagtact gtgccgatta tcatgctgac agctagagat tctgaaagcg
     1561 atcaggtgat cggcttagag atgggggcag atgattatgt cacaaaacct ttcagtcctc
     1621 tgacgcttat tgctcgaatg aaagctttgc atcgccgtag tgagttggca gaagcaagag
     1681 aagaagcagt agtttcagat gatcattttg atatcgagac tagtcatttc aagatgaata
     1741 cgaaaacacg agaagcgtat ctggacaacc ggccaattga aggactgacg cctaaagagt
     1801 tcgatctatt gtttacttta gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc
     1861 ttgaattagt ctgggattat cagtactttg gtgatgaacg gacagtggac gcccatatca
     1921 agaaattacg acaaaaaatc gaaaaggtcg gaccacaagt catccaaact gtttggggag
     1981 tcggctacaa attcgatgat tctggtgttg cctaatgcgt tatttgtatc agcaactctt
     2041 agctttctgc ggaatggtcg cgttgatctt attgatcgtt ggagtttctt ttacacaact
     2101 gacaaaacaa acgattgaag aaaacaatta ccagcaactg tttggatatg ctgaatcagt
     2161 tgaaaagaca acgcaaacct atgccgatac gctcccgcag tttagtcaag atcaggcgtt
     2221 tcaaaatgca ttgctgctga cagagcaagt actgacagaa caaaatgtga actttatatt
     2281 tatcgataaa aacgaacgtg tcatttatcc gactaataca gctttactga attttacctt
     2341 aacagctgat caatggaaaa atctgaggaa tggacgtcaa gaaaaattca catcgaataa
     2401 aaatatttta ggacaagcac aggcgacctc ttacgcactt gtgccattta atttgaatca
     2461 tgaattctat ggagcacttg tagttagtca acctgcgcga aatatcgata acagcgttcg
     2521 tccaattaca ctgaacctgt tcaaaggatt tattttttcc agtatcgttg cagttattgc
     2581 cagctatgct tttgcagcat tccaagttaa gcgtatcaat cggttaagga atgcgacgaa
     2641 agaagtgacg aatggtaatt ttgatgtaca gctccccgta catgacaaag atgaatttga
     2701 tgagttagca gatgatttca ataaaatgac gaattcattg aaagaatctc aagcagaaat
     2761 tgaagaacaa gaaaatcgtc gaagacaatt tatggcagat gcttctcacg agatgagaac
     2821 acctcttacg acaatcaacg gacttctgga agggttagaa tacaatgcaa tccctgaaaa
     2881 tcagcgagaa aacgcgatta aattgatgaa aaatgaaaca gagcgtctga tccgtttggt
     2941 gaatgaaaac ttggattatg aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa
     3001 attcaatgga acagaaacat tgaaaaatat tgttgcacag ttagaagcta aagcagaagc
     3061 agcaggagat acattgacat tgaaagccgt tgatgatatt gatgtttacg ctgattatga
     3121 ccgatttgtc caaatcatgg tcaatattat tcaaaacgcg atacagttta ctgaaaacgg
     3181 ccaaatcacg attactttag aaaaaggcta tcttgaaacg atcatcacga ttgaagatac
     3241 gggaattgga atgtctgaac aacaaatgaa aagtatctgg gatcgttatt ataaagtaga
     3301 tccttcaaga aaaaatacaa aatatggtga atcaggatta ggtttaccaa tcgtgcagca
     3361 acttgtacgc ttgcataaag gaaaattaga agtagaaagc gaactaggaa aaggaacaaa
     3421 attcgctgtt cgtttgcctg acgtagaaat caaaaaagag taaaaaacaa gcggtataaa
     3481 agatcatgaa tcgatcttct ataccgcttg tttttactat aaaatgattg atttagcccg
     3541 cgggctctaa acgatcaata taaggacttt gagcaatagt agcatcatga gtaaagagta
     3601 agataccttg attttcttca tgagctaaat aagaaagcaa gtggagtagc tcttggatct
     3661 ccaatacact aagttcatca aatgtatctt caataacaat attgtttcgc ttggaaagca
     3721 aggcatgaag taattgcagc tttacttttt caaaagacgt caactcagct gccgcacaat
     3781 taagcaagga aggagagata tcaagttttt cttgataggc caaacgatct tgtttgattt
     3841 ctttttccgg tacatccaac aggacatggt ctttaagatt cagataagga aacatcggcc
     3901 aatttttttg gatgatcgca aaattatcca gttttgcaac aagaacctct tttttcaagt
     3961 gctccaatat tttatgctgt ttttcgagag gacacaaaat aacggtcaag tgttcagcat
     4021 gaatcaaata ttcaggtttc attacgtcca ggcctcctac tttgcaaatg atggataaaa
     4081 aaagtgaaag ggaatagact aagaatgaga cagcatccac tgagcccaat aagagtttgg
     4141 gcaaatccct taagtatatc tgtgaaaaaa agatcatttt ggaaatcgat ggtaaaaaaa
     4201 gtctgttgat ccatcggaat tgtaagtccg cttaccgaag tcaagatatc acggatatct
     4261 ggcagttcag tagtctttaa gacaagaaaa ttgatccttt gaagaagcgc ttcataaact
     4321 gactggaagc ttacgatcca caataaaacc aagatacaac aaagaaagag aggaaagaac
     4381 agctgccaga aaataaaaag gaataatttg gttttagaaa gaccagtcag gtgccaattg
     4441 atcaattcct gcttgttttg cctgactgaa tagtaaaaga aaaagataaa aaggaaggca
     4501 aatagaaaaa ataaactgaa tatgcctttt atataaaatc ggctagctaa ttctaaagcc
     4561 ggaagaagct ctgtagatgc agaagcgcca ataaatgaaa gccgatcttg gacttgattg
     4621 gctaaagccg attgcaaatg gtaaagagtc aggcatgcta tcaataaaaa agcagaaaag
     4681 cttgaaaata aacaatagag tattgtctgc tttttgtaat aaattaatcg agcagtagca
     4741 tagcggatta ttttcatact tttcacctct ataaaaagta tagaaaatga atatgaataa
     4801 agtatgaaat tcctttagtg aaatcacgaa ttaaaaccag aaaatatttt gtaaaaagca
     4861 agaggttgtg aagaagctgc cctattctat taccgtagat gcaagctttt gaacactgtt
     4921 tattcgaaaa gaggagcagc gacaataatt t
//
LOCUS       DACEPW010000162         4922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEPW010000162 DACEPW010000000
VERSION     DACEPW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.166"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2612)
                     /locus_tag="IW819_002255"
     CDS             complement(<1..2612)
                     /locus_tag="IW819_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1245344.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNAKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAALFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLS"
     gene            complement(2622..3323)
                     /locus_tag="IW819_002256"
     CDS             complement(2622..3323)
                     /locus_tag="IW819_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245345.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            3508..4107
                     /locus_tag="IW819_002257"
     CDS             3508..4107
                     /locus_tag="IW819_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1245346.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILSNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTNN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHVVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4188..>4922
                     /locus_tag="IW819_002258"
     CDS             4188..>4922
                     /locus_tag="IW819_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1245347.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGV"
BASE COUNT         1425 a         1005 c          899 g         1593 t
ORIGIN      
        1 gataagacat aagcccacca tgtgatcact aatggaaaga tgacattctc catagacgct
       61 tgttgcaaga tgaagtcagt caatatatat cctacaccaa atcctccgat gattcctaaa
      121 agagtaaaga tgatattttc tcgaacaatg tacatcgtga cttctttatc aaaaaatcca
      181 aggactttta ttgtagataa ttcgcgtaca cgctcggaaa tattgatatt agttagatta
      241 tacaagacga taaaagccag cagtcctgat aatacgacaa agattaagac aaccgagtct
      301 aagttcgcca ttgattcttc ttgcgtttcg atttgtttgg acatgaaaga cgtatttgtc
      361 acagcgtctg tggcaagcag tttctcggaa agcatttctt cttcttttgt tgtcatctta
      421 tctgtacgca gcaaaaatgc attatccatc atcgattgat cagtaactga agcataatat
      481 tcaggggaca tataaataaa gctacctaga tagtttttca cgatttcttt tacttcgact
      541 ttccattctt tttgatcttc atcataaagc gaaatatgat ctcctgtaga taagtcaaat
      601 agttctgctg ctttttgcgt aatagcgatt cctgttttag gcaaggagcg tgggttctct
      661 tcaagatcag ttaaatggat aaaacgatcc aaatcagctg ttttacttgg tacgatcact
      721 gtcagacttt gcgaagcatg tccttttttt ctcaattcta cggtttgagt atgaatagct
      781 aggatctcat ttactttttc ttgcttctgc agcatatccg ctgctttttg acgactatct
      841 tccgtggatg aatccaatgt aacgattgct tgatagtcaa tgatcggtcc aaattgtttg
      901 gctgagacag aacttagaga atctttcaat cccacgcctg caaccatcag cccggcacat
      961 ccagcaattc cgataatcgc cataagcatt cttgacttat atcggaagag atttcggtaa
     1021 ctgatttttt gattaaagct aaggcgtgac cacagcggag tgatgtattc aagaaaaatg
     1081 cgtttccctg gcttcggtgc tttcggttga agcaatgcag caggccgttc tcttaaatct
     1141 ttgaataaaa cgatcattgc agcgccaaat gcggcaatga agagtgcggc agctgcttgt
     1201 aaaatcggcg gccagtcata aaaaataagc gcactcccaa tatcataccg ctcgttagac
     1261 agctcaaaaa tgatccttgg caaaagattc gttcctaata tcgttcctaa aacaatgcct
     1321 atccctgagg ctagacttgc atagattgca tatttttttg cgatttcaac tttcgtataa
     1381 cctaacgcct ttaatgtccc gatttccctg cggttttctt ctaccatacg tgtcattgtc
     1441 gtaaaggtga tcaatgcagc aataaagaaa aagaaaacag gaaaaacatt ggcaatcgca
     1501 gcgatacgat cagacagatc accaaattct tgaaaaccga cattttctga acgttcattg
     1561 tataggtatg atggtttagt taaattttca attttttctg catttcgatt caattcttcc
     1621 tctgcagagg ccagttcacg ttctttctca actaattgcc tttcttgttc agcaagttga
     1681 gcttcttgtt gagataatgt ttgaacagcg attgttcgtt gcgcttcagg aagctgctgg
     1741 atagttgctg actgttgcgc taactgtttt ttagcatctt ctaaccgtgt tcttgcttgc
     1801 gttgtttgtg ctttgccatc cgctattttt tgctgtgccg gttctaattc ttcgttggcc
     1861 gattttttca gctcttctaa gcgttcttcg ggtcgatctt gtaaatactt ttctgccttt
     1921 tcttgatttt gctccatttt atctttgtaa gctttgctgt acgtattcaa attctttgcg
     1981 tttgtaaaat caagatataa gacagaataa atatctgttt taaaaacgga ttcaggtaaa
     2041 tagacaaaat aatcgacgct accatttccg acgttcgcgt atcctctctc caaattgttg
     2101 atgaacaatg gagaacggac aaagcccgtg atggtatact ctttttcttt caattcatct
     2161 gaatcaatct gataaatatc accgatttta tacccttcat cttccgcttt ttcatccaat
     2221 acaagctggt ttggtttttc cgggagtttt ccatcagtta attgtagctt gttctgcttg
     2281 ttatttttgt tataagaaaa tatttgaatc acttcgtttt tgccagtatt ggaataatag
     2341 atctgttttc ctgtttctac ttttgcatct gggatctttt ctgcttgagc aatatctttt
     2401 tgtgtcaagc caccagtaga aacgatttgc aaatcagaaa gtcctgctgt accaacatag
     2461 tgatccatcg ttttttgcat gacaggtcca gcagttttga cacctacaaa taaaagtgtt
     2521 cctagcatga caataaggat gattgcaatg aatcgtcctt tagattgccg aatttcgcga
     2581 aaacctgctt taagatatgt tcttgttttc ataaaatccc cctaccatac aatctcatca
     2641 atggatgaag gatgatcatt taattcaaca gaccttactt tagcatcatt aatgtgaatg
     2701 actctatcag cgatgggcgc taaagcagaa ttatgtgtaa tgatcaaaac ggtattgccg
     2761 tgttgtctgc tagcgttttg caataatttc aatacttgct tgcccgtttc aaaatccagc
     2821 gcgcctgtcg gttcatcaca taaaagaagt ttgggatttt tagctagtgc acgagcaatt
     2881 gaaaccctct gctgttcgcc tcctgataat tgggacggaa agttattcaa tcgatgagct
     2941 aggcccactt gcctcaatac ctctacagga tcaagcgcat caggagaaac ttccgttgcc
     3001 aattccacat tttcttttgc tgttagatta ggaacgagat tgtagaattg gaaaacgaac
     3061 cctacatctg ttcgtcggta agcagtaagc tgtttatctg aaaattgcgc aatatctgtg
     3121 tcatcaataa tgatctgacc ttcatcaggt gtatccatcc cacctaaaat attaagaatc
     3181 gttgactttc ctgctccact tggaccaagt ataatcacta attcgccttt ttcaattgaa
     3241 aaagaaatat cgtcatttgc tgtaatggtc gtttctccca ttttgtatcg tttgtattca
     3301 ttttttactt cgacatactt cactctgatt cctccatttt cacgatattt ctcgctatat
     3361 ctatgcatag aatgcgtttt ctcacactta tttattttac cactgaattt ctctagacta
     3421 aagaaaggaa tcctttattt agttgacaaa taaacgattt atgatttact tacataaaaa
     3481 acattgttat ttgaaagaag gaacatcatg aatgatttaa aggaaaatat gatttttgat
     3541 ttatcaaaaa ctgcttttgt cgcgatcgac atgcaaaaag ggatcgtaaa taacggtaca
     3601 ctctctccac acactgcaca atctattctg tcaaacaatg atttgttagt aagaacattg
     3661 aaacgtaccg ctgccttgaa agttttagtg aacgtagaca tctccacttt cccttatttg
     3721 agtcaacaaa ctgatatggg tggaatgact gcttctgtac ctccagaatt cactgacttg
     3781 ttgttgaagg attccttgaa agatacaaac aatatgttga ctatcacaaa atacaatcca
     3841 agtgcctttt ttggtacttc tcttgatctt caacttcgcc ggcgtggaat cgaaactatc
     3901 attttaagcg gtgtagcgac aacaaacggt gtatatgcta ctgcattaga tgccttccaa
     3961 catgggtatc atgtcgtact tgctgaagat gcttgcagtg atcgtgacaa agaatcccat
     4021 caattgttca taaaaaagat atttccgaaa actgcacgag tacgctcaac gaaacaaata
     4081 atcgaagcta tccaacaaag caaataagta ctggcacacg tgattttcat ttacttcttt
     4141 ttgatttcat gggaaaataa ccacaggatt tagataggag tatgaacatg gtgaaaaaaa
     4201 agatcacaat caaagaagtc gctaaacttt ccggcgtttc aatcacaact gtctccttga
     4261 tattaaatgg aaatacggag aaattcagtc cgaaaactgt tcagaaagtt ttagctgcca
     4321 aagaagaatt aggctatcag cctgactatt ttgctagaca aatgattacc aaggaaacga
     4381 aaacaattgg tgtactcgtt cctgatatca cgaatccatt cttcagtaca ctgatgcgag
     4441 gaattgagga tatcttgtat aagcagaatt tcgtcacgat attatgtaat gctgattctg
     4501 atcatcaaaa agaaatcgaa tatctagccg agctcacacg tcgaggtgtg gatggattca
     4561 ttatcgcgac ttctgctgtc tctacagatg caatcaacga aaatttgaaa aaacaaggcc
     4621 ggcccttcat tgttttggat cagaaaaaat ctgaaggatt tagtgatgcc gtacgaacag
     4681 acgattttcg cggtggttac ctagcgggca tgcacttgct tagtctaggt catcaaacga
     4741 tcgcccttgt ctatcctgaa aatccgccgg aaaatgtcca cgctcggatt gaggggttta
     4801 aatctgctct tgatgtttac caaatctctc acgaccaact gatcctctta cctacacagt
     4861 tctcaaaaca aggtggctac caaatcacag cagaacttct tgattctgct gcaactggtg
     4921 tg
//
LOCUS       DACEPW010000163         4813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEPW010000163 DACEPW010000000
VERSION     DACEPW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.167"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            560..1327
                     /locus_tag="IW819_002259"
     CDS             560..1327
                     /locus_tag="IW819_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ1245348.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            1353..1898
                     /locus_tag="IW819_002260"
     CDS             1353..1898
                     /locus_tag="IW819_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245349.1"
                     /translation="MGERTQALITVKYGNSKAKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            1899..2465
                     /locus_tag="IW819_002261"
     CDS             1899..2465
                     /locus_tag="IW819_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245350.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            2480..2728
                     /locus_tag="IW819_002262"
     CDS             2480..2728
                     /locus_tag="IW819_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245351.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMLGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAEYIFSWLGQ"
     gene            2748..2987
                     /locus_tag="IW819_002263"
     CDS             2748..2987
                     /locus_tag="IW819_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245352.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKAQRKGYPDKIFKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            3004..3588
                     /locus_tag="IW819_002264"
     CDS             3004..3588
                     /locus_tag="IW819_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245353.1"
                     /translation="MNEDKMVLSEYARLRTINWLNGMQSSGAFDGFDEENINFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEQELILERQRILRSEQDSKILYGLYDLQLNGLKAQYV
                     ADSFDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITKEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYEKMKERLKSKVES"
     gene            3695..3976
                     /locus_tag="IW819_002265"
     CDS             3695..3976
                     /locus_tag="IW819_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ1245354.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFNEA"
     gene            3966..4307
                     /locus_tag="IW819_002266"
     CDS             3966..4307
                     /locus_tag="IW819_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1245355.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAISKSRENISFKKVGMLSASDTKKL
                     NRFIERLQRKV"
BASE COUNT         1822 a          590 c         1047 g         1354 t
ORIGIN      
        1 ccgtcctagc catcgcctag tccgattttg cttgttcggt tccatttctg tttatttagt
       61 gggtcaacac cgtaatctta aatagccatc gcctatttaa gattactagg tctgctttga
      121 aggtaaaaag tctttcattc ttcaagactt ttaagctttc aagctacgcc tgaaagcatt
      181 gaaattcatc gctctttatt tggttttgcc atttataatt ccttacgtcc attatctttc
      241 ttgcctgtca tgccttgtca gaagtgttta gagcttatct catctttgga ttaatacaag
      301 tggcgtaaat ttttttctaa acgcaagcgt tacgaaaaaa atttacttaa acacttgtat
      361 taatccggca gataagaacg cattctaatc actcccgaca aggcaagaca ggcgaaaaag
      421 ataaataaat gaacgaaagg aagaaacata aaatgcaaaa ccaaatagcg atgaagaaaa
      481 aacctcctcc ctctaaaaca atccaaacaa aaaagaattt tgaaaaacca aaaatcaaaa
      541 acataaagaa gcgttcaaca tgagagatag aattttagaa gtaaatcaag aaatggtaaa
      601 caagttaaca aatatcagtg gaaactatga aaagtacgat ggtactttac ttgataacta
      661 tatttttcac gatacacaaa atatcagaat tggcagaagt aaaccgagag cgttcattct
      721 catcaaagag aaatatttga atgaatggaa tagcacctat gaaatggtta tgactgataa
      781 ctttgaaaag gtattggatt tcagaaatgc tttgttgggt tctgatatgg gaaatagcag
      841 tcttgaatct attgaagttg aaaacaagac cgagaatgaa ggtgaaggat tgattaagaa
      901 tgtcctgaaa atcgaacaaa aatatatcag tgaagataga agtttttata gagtcacaag
      961 ctcggttggt ctagcaaact ttgctactaa cttaattgga aacaatgcta gtgaatcttt
     1021 gctagtgatg tgtctgaata caaagaatga ggtcattgct tatagcgaag ttttcaaagg
     1081 tggaattaat caatcagtag cgataccaag agagattttt caactagctt tgctaaacaa
     1141 ttcagcaaaa atcgcagtat cacataatca tccgagcgga tatgaatttc caagtgaaaa
     1201 tgacaaaagt tttactacga atttgaaaaa cgcaggaaaa acaattggta ttgaattgtt
     1261 agaccatatc atcgtaacaa gtaaaaacac aagctattac tcatttcaag aaaatggttc
     1321 tttataaaaa ttagagaaaa gaggaaaaca agatgggcga aagaacacaa gcgttaatta
     1381 cagtcaaata tggtaacagc aaagcgaaag caagcgtgtc aattcattat cagtggggat
     1441 atggtcgata catgttgatg gacgttttga atcaaggaat atatttaatc aataaagcat
     1501 atgacttacg agatgaatca agagaagaat tagcaaaagg aatctttgat agaagtacag
     1561 gagttatcaa ttttgtcacc tatgaatctg gagtgagtgc ttatgatgaa acagggtttg
     1621 gtgggcgcta tgaaccttat caagaaacga ttgaagatgt gatgaagttt tcagataaca
     1681 atgatggcta tgctcattta gagatggtgt tcgatgaaaa tggatttagt ctagatgata
     1741 aaaccagatt gcgattgttt gattttgagg gtcaacagat aggactaaac aagttttgtg
     1801 aaagcggaaa agagtttgct acgaaagatt ttattaaggg atacagatta ttgctagaaa
     1861 attatggaat aaacctagaa gaagagagcg tgaaataagt gtttgaaaaa agaaaagacc
     1921 agttccctaa tttagaagcg aaaagtcaaa aagtgattga tggtgaaatg attgaattat
     1981 tccaaaaaag ttatggaacc atagaaacta ccgcaagttg caaatactac tttcaaggaa
     2041 agagaaacac gtttaaaggc tttgccgatt ctgatgaaga attggcaaag aaaatcattg
     2101 attggcttgt cagcagtgaa cacgtagcaa gagaagatac cgttagaaca acgattaatg
     2161 gggaagtcct aacaggtact agatgtgttt gttgtaatca tgaatttgta gaagatgaag
     2221 gttgctaccg aatcaatgaa tatggtgatt ttatttgcca gagttgccac gagatagaat
     2281 ttccaaccga aagaagttgg tttgaataca tgctacaaga ttatgagatg gaaaaagaaa
     2341 atggtgaagt agttttttgt accaaagagg aattgagtaa gagaagcgat gatgaaattg
     2401 aagattggtg tgtgtggtta gtaggggcag atgattgcgt tgcatattac acaacagcag
     2461 aataggagtg tgaatcagca tggaaggaat ggattttcta gatcatgaag atttggttga
     2521 ttttggttat acatggaaag gaatgttagg tattagcaga agtcttgcta atgcctttta
     2581 cgaaagaaat tatgcagtat atgttcttta tgatgacgat acagaaagcc ttgttgacga
     2641 ggaatataaa ttggatttag agaatgtttt atatggaata gaaaaggaag atttagcaga
     2701 atatattttt tcatggttgg gtcaataagt gaaaggaaga tagaaacatg acaaaattta
     2761 aaagtaaggg tatgcaatgg tgttcttatt attatgttgg aaatgatgaa gatttattga
     2821 gaaaagccca acgaaaaggt tatccagata agatattcaa acaaataggt tattacatgc
     2881 cagaagagag gatagaaaaa gaattttgtg aggttctttt tgatagtgaa cagctattag
     2941 ttttgttaac ccaaaataaa ttgatttgcc aatttgaaaa taattaagat gaggtggtga
     3001 accatgaatg aagataagat ggtattaagt gaatacgcta gattgagaac tattaattgg
     3061 ttgaatggta tgcaaagtag tggggctttt gatggatttg atgaagagaa tataaacttt
     3121 gaaagtttat cgaatgagga tattagatat tattttatcg aatggtattt tacagaacca
     3181 gatgaagagg tggaagatga gcaagagtta attttagaac gtcaaagaat tttacgcagt
     3241 gaacaagata gtaagatatt gtacggttta tatgacttgc aactgaatgg attaaaagcg
     3301 cagtacgttg ctgatagttt cgatgatatg gtagaacagg gggcagatag ggttgtcact
     3361 tattcagaag atgatgaaga ggaatgggaa aactattcag atgatgagat atgctttaat
     3421 gatgggtata tgcctattga aatcaccaaa gaagaatatg ataaagtgtc cgatagcgat
     3481 gagattggta tgtgtacggt tgtaagtgga ttggatatag atgtccaaag agtagaggtt
     3541 gcatacgaga agatgaaaga acgtttaaaa agtaaagtag aatcttgatt tgattggatt
     3601 gtttgcaaag tagctaatta agagtataat ataatcataa atgtagttaa ttgatataat
     3661 aatgagtata ttatactcaa aagaggtgaa cgaaatgaag gtcaaaaatc caaaagaaaa
     3721 aagcagaatt caagtcggaa tagacaagga tttgaaagaa aatgcagaaa tgatattgga
     3781 agagctaggg ttaaatccaa caacagcaat cacaatcctt tataagcaag tggtcgctag
     3841 aggtgagttt ccagtagaga taaagctaag cgaagaggaa aagcaaggaa tcagattgca
     3901 acagctaact aaggatatgc cagttgatgt tctagataca gatgaaaagt tagaagagtg
     3961 gttcaatgaa gcctaatgag atagtaacgg tatatgttgc atttacagat aaaaatggtg
     4021 gtaaaagaag acctattttg gtggttagtg ataaagaaga tagagtagaa ttctttggaa
     4081 tcacaagtca atatgagaaa aaatcagacc aaataaagag ggtgtatttt cctattgcag
     4141 aatgggaaaa agcaggactt aaaaagcaat catggattga cgtagggtct ttaaaggcta
     4201 tttccaaaag ccgagagaac atatcattta aaaaggttgg tatgctatca gctagtgata
     4261 cgaaaaagct aaataggttt atcgagagat tacaaagaaa agtctaacaa ggcttttctt
     4321 tttttgctta tatgtatatt aagatatacg tctatataaa cgtatttatg tatataaaaa
     4381 tacacgtaaa tatatacatg aatataaata ttaagaaaat attaagagtg attaaatatt
     4441 gataattatc aatatacgaa ttataagata tattttatat caaatgtatg taaataaata
     4501 aaatgactga cggatattga atagtaataa ataacagtaa tccagacgga tacagtgagt
     4561 gaagtagctt gctacgaacg aataggcaga atgaaacggt agtttcatta gagcaagtcg
     4621 cttgcgacgt agcgttacca ctgatgtaac tggcaagtta catagagaga gtttgcttgc
     4681 aaacgaacgt tacctacttg aatagaaatc aaaaataaca gattagtgaa ttttgaatag
     4741 tagccacaac tcacccgacc gcttgcggtg ggtgagtccc acggaggaaa agacgaacga
     4801 acggtcgcgt agc
//
LOCUS       DACEPW010000164         4764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEPW010000164 DACEPW010000000
VERSION     DACEPW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.168"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..678
                     /locus_tag="IW819_002267"
     CDS             <1..678
                     /locus_tag="IW819_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1245356.1"
                     /translation="LKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGTNQYSVDSSK
                     FELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLSTKNNKLDEE
                     LIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKI
                     IYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQI
                     SEGIDKQ"
     gene            754..972
                     /locus_tag="IW819_002268"
     CDS             754..972
                     /locus_tag="IW819_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245357.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            959..3637
                     /locus_tag="IW819_002269"
     CDS             959..3637
                     /locus_tag="IW819_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245358.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3628..3825
                     /locus_tag="IW819_002270"
     CDS             3628..3825
                     /locus_tag="IW819_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245359.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3829..4440
                     /gene="mobC"
                     /locus_tag="IW819_002271"
     CDS             3829..4440
                     /gene="mobC"
                     /locus_tag="IW819_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1245360.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4425..>4764
                     /locus_tag="IW819_002272"
     CDS             4425..>4764
                     /locus_tag="IW819_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1245361.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHF"
BASE COUNT         1836 a          649 c          934 g         1345 t
ORIGIN      
        1 ttaaagcctg ttctgtgcaa agatgatgtt tataaaatta ataagataac ccatcaagaa
       61 aatagacaaa aaaacttgat agttcaaaat ttgaactatg gaacaaatca atattctgtt
      121 gatagttcaa aatttgaact atgcgacaca tcaatgaaaa caagagacaa aaacaatttt
      181 gctgatagtt caaaaaatga actagaatta tataagtctt ataaagatag taaagatatt
      241 aaagaatcac ataatgatga tcttcttgaa tcactttcaa ctaaaaataa taaattagat
      301 gaagaattga tcgaaacatt tattgaagaa aatcttctta aacaaatatg gggagaaagt
      361 attgtgaatt gcttaaaact ggctagcaat agtgattatc gtcaattcga caactggtac
      421 aaaaaattca aatatgcgat tagatctgct gaaaaagaac aaaaaattca actgaaaatt
      481 atctatgaaa tttgtaataa caagtatttt gtagatcatg ttagagaaca gctatcaatg
      541 actttacgtg acttgatacg aagagcaaaa acagatcatc gtatcaaaca aaaagacgat
      601 tatatctttg cttctctaaa aaataaagcg ttagaactaa ttgaattgca aatcagcgaa
      661 ggaatagata agcaataaga atagaattga gtcaaatgaa atggaggggt atctccactt
      721 catttgagaa ctttgaatat aggaggggtt attttgatta aaaatcgttt aatagatcaa
      781 gtttggtgtc tagatcaaaa tacagatagt ctcgaattaa tcgagactat ttgcttcaac
      841 actatagttc tcgatttaag agctgaaaat gataattgtg taactcatgt cataattaat
      901 caaaaaatgg ctcagaagct ctctgagagc ctcagaaaat gggctaaggg aggtaacgat
      961 gaaaataatt gaaaaaaaga ttgccaataa aaaaataaat tggttggttg ttaattttct
     1021 tgttctgata gctatgatta tttttacaag taatattgct catgcgagta ttttcaattg
     1081 gtttgatgac acaaaaagtc aaacagagtt tttgaataat tctctttata gaccttacct
     1141 aaaaaagtac aatggagatt tccttagtca gttcgggatt tggctcggat gggctgtggt
     1201 aaaaggcatg tttactgtga cagattcgat tcaaaatatg attccagatg tgctggattt
     1261 gttcaatttc attgaaagta cagggctaaa taatgtttat caatcagccg taaatacaat
     1321 tgtcattgga ttaatgatcc tttcattgat gtttgttggt tacaaaatga ttactggaaa
     1381 aggaacagtg gatttaaaat cggttggaat gaacgtagtg atgtctatag cattaatact
     1441 tttaatgcca accatgattt ctagtggtat tcaatttgct aaaacatttt ataaggactc
     1501 tactacgatt acaaattcag atgacggagt cgcttggtca ttgataaaac aaggagtaac
     1561 agatttagca tatattaata aaacagatca gtactcaagc attgataaaa cggaggatcg
     1621 aaataattta acacgaaaaa attttcaaca gacggatctc acacaagtgt taacggataa
     1681 agtgattgat aaattggaaa aagaaaatcc aattgcggac aatcttcgtt atgaattagt
     1741 tgaaaattct aataatgaat ttgttgctac aaagttttct gacaacttct tatcaacatt
     1801 ttctgatagc ttgaaatcag gctattaccg ataccaagca aatctatggg ggatttcaat
     1861 tgggttgaca gcgttagcaa tcgcctatgt gttcagcgcg tttgtgatca ttacagcaat
     1921 actagaactt gcatttaaac gtgtgttagg tgttcttgtt tttgcaacag atattgagac
     1981 aggacagcgt tcaaaagttg ttctatcaga tattctacaa tgttacttaa ctgttgggtt
     2041 tcaaggattt gggttatcaa tgtttgctat gtttatcaat tttctgaact caggacaagg
     2101 tatctcaaca aatattttta taaaaacaat tgcttatatc tgtgccgttt ttgttttaat
     2161 caaaggcagt ggaacagtca tgagatactt tggtgttgat attggactca aagaaggcta
     2221 tgggcaactt gcttctgctt ttggaatggg cgcaatgctg tttagaaaag gatcaaatgg
     2281 atttaatcgt gctaaaggaa gcggagaagg agaagatcga aaaccagaaa agaatttcgg
     2341 cgaaactcta tcaaaaaaag ctggcaaaac tggaagagct ttgggatatg cccatgaacg
     2401 tggattatcc ggtttagctt cagatggagc tacaatggct agtgaaagag caacaaaacc
     2461 atttaagagt atgcgtgata tagctaatga taccaagaat aagtttaaag aaggtttaga
     2521 tgatggtaca gtgtccgcta ttaataaaaa tagcaaacct atgttagcta aaaataaaga
     2581 agatgaaact ggaaaatatg ctgattccgt gccaacacgt ctttcagata gaaaagatgg
     2641 tgctaaagtt gaaaatgccg atagaattat gtctagttct gaacgtatgc gtgaagcaat
     2701 gaaaaataat gctgaatcga ataataatcc tgcaagcgca attcagcaaa aagtccaaca
     2761 agatattgaa gaacgtaaaa atgctatgca cggtcaagct aagtctgctg aagaattaat
     2821 taatcagaag cgtcatgaag cgaaatacac gcctgaagca atgaatcgcg aggaaataat
     2881 gttaaggaaa cgtgtagaag ggcggacagg cgctaatatt gacggaaaag aagcgttgac
     2941 caaagaaaga attcaagaag ctaaaaattc aaatacagga ttagaaaaat tagtcaaaga
     3001 aaatccacaa aattctacaa gtagtcatgg tggccgatca gtagatgtcc gagaaaatat
     3061 tcaaggttca ctgaatggtc atgttggacg gaatgttgat gtaagagaga atttacaaag
     3121 ttcttcaaat ggtcaaggta ctaagacaat tgatgttcga gaaaatctgc aaagtagcag
     3181 tacaagtcaa ccaaaaacag tagatgttcg agaaaatgta caaaattcta caagtagtca
     3241 agggtttaaa tcagtggatg tccgagagaa tgtgcaaggt agcagtgcaa gtcaaccaaa
     3301 aacagtagat gtccgagaaa atgtacaaag ttctacaagt agtcaaggat tcaaaacagt
     3361 agatgttcgg gagaacattc aaaaagatag tggagaaatg aaagaaaaaa cacaaaaaat
     3421 caatatcgtt gaagacagaa agtcagcgaa aaaatttgat tcaaatcatg aaacagtgat
     3481 tattgatagt gaaatccgtg aaaatgataa gggatcaaaa tcaagacgaa gattcactta
     3541 tgaagacaat gagttattca gagatacact taatgatcct aatccacttt tcgataattt
     3601 attaagaaat aaaagggggg gattgtcatg caattaattc taaagattag tacgcatgat
     3661 ttcttagaac ttgacgaatt atgtgaatca gtaaagaacg caacaaatga aaaaatcacg
     3721 gtcactaatt atattgaact gatgattcaa gaagaattaa agatgaaaaa tgaaatagag
     3781 agcaatttca aatgttgctc tctagaaaaa aatgtgaggg gataatcaat ggatgataaa
     3841 caaaaaatgt acgaaaaaaa tcgcaaacat agtgaggcct taaattttcg tgtcgataaa
     3901 aagacttatc ttgaactgac attgattgca aaacaaaggg gaatttctag agcagacgtt
     3961 tgccgtgagc ggttaaaggg actcaaatat gaaccgccgt tgttagctga agatgatagg
     4021 gtagttttaa ccaaacagtt aaatcaaatt ggtaacaatt tgaatcaagc ggtaaaggca
     4081 ttaaatatca ttgctaaata ttttagagaa gaatctgaag ataaaaaaga aaagagccta
     4141 cattttagtt ttgatgctag ccgttacaac atttttaaag atgaacttgc gaatagcata
     4201 gcaaaaaagt tgatgattag ttatccagat gacatttttt tggagtccaa tcggagtgcc
     4261 ccctcttttt ttaaattatc agaagtagaa aaagaatatt tcgaatattg gatagaaaag
     4321 caaactagac cgggtacgaa agaagagtat tcccaaaaag ttgatcggaa tttaattcac
     4381 tcaattcaga ttattgcaag tttaaaagat gaggtgaaac aactttggga gctactataa
     4441 catcgaaagg acgcaagaaa aatttatcct cgctattaca atatgcagct aatccttcaa
     4501 aaaaagaaaa atgtgttgca gtctcaggaa taaattgtag tgatgatagt gaggaagcac
     4561 tgcaagaaat gaatttagta cgtaccaaga tgaataaatc taaagatata gcacaagcgt
     4621 ttcatgttat tcattcgttt gataagagta cttctaaaga gttaagtatt gataaaatgc
     4681 acgaaatagc tgtcgaattt gcagagaaag cttttccaaa ttcgcaaatc attgttgcaa
     4741 gtcacaatga taaagatcat tttc
//
LOCUS       DACEPW010000165         4761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEPW010000165 DACEPW010000000
VERSION     DACEPW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.169"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            7..1398
                     /locus_tag="IW819_002273"
     CDS             7..1398
                     /locus_tag="IW819_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1245362.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="IW819_002274"
     CDS             1414..2454
                     /locus_tag="IW819_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1245363.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="IW819_002275"
     CDS             2473..3558
                     /locus_tag="IW819_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ1245364.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="IW819_002276"
     CDS             3591..4553
                     /locus_tag="IW819_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1245365.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..>4761
                     /locus_tag="IW819_002277"
     CDS             4546..>4761
                     /locus_tag="IW819_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HAQ1245366.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLL"
BASE COUNT         1735 a          644 c          855 g         1527 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact g
//
LOCUS       DACEPW010000166         4759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEPW010000166 DACEPW010000000
VERSION     DACEPW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.170"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            544..1107
                     /locus_tag="IW819_002278"
     CDS             544..1107
                     /locus_tag="IW819_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1245367.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IW819_002279"
     CDS             complement(1386..1673)
                     /locus_tag="IW819_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1245368.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IW819_002280"
     CDS             complement(1663..1992)
                     /locus_tag="IW819_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1245369.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IW819_002281"
     CDS             2297..2626
                     /locus_tag="IW819_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1245370.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW819_002282"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW819_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1245371.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IW819_002283"
     CDS             complement(3551..4462)
                     /locus_tag="IW819_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1245372.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1559 a          816 c          719 g         1665 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgacc
//
LOCUS       DACEPW010000167         4750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEPW010000167 DACEPW010000000
VERSION     DACEPW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.171"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(698..1510)
                     /locus_tag="IW819_002284"
     CDS             complement(698..1510)
                     /locus_tag="IW819_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1245373.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1673..1780
                     /locus_tag="IW819_002285"
     CDS             1673..1780
                     /locus_tag="IW819_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1245374.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1838..2905
                     /locus_tag="IW819_002286"
     CDS             1838..2905
                     /locus_tag="IW819_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1245375.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            3047..3250
                     /locus_tag="IW819_002287"
     CDS             3047..3250
                     /locus_tag="IW819_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1245376.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            3310..4512
                     /locus_tag="IW819_002288"
     CDS             3310..4512
                     /locus_tag="IW819_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1245377.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1580 a          748 c          987 g         1435 t
ORIGIN      
        1 aatattattg tagtcttttc tttattagga atcatcactg ttgtatctta ttaccttttc
       61 tgtttttctt attagagaaa aataatttga gaggtagata gtttttattg gaggaagtaa
      121 ttatgtatat ttttgaaaca aaaatttttc attgcaataa ttgcaacaaa gaaatttcat
      181 cgggggaaga ggtttggaca aaaatggaaa tttccgccaa aagtaactgc tcctcaactg
      241 aaatcaatta aagaactaga atttgagaat gcactggtaa tttgtatgac atgtactgat
      301 gaagtatttc gaaaacattt ttaaaggttg agatgtatat agatttaacc aatcatttat
      361 ctctctcgga aaaggaatat taactaacca gcgggttatc aaaaataggt aggaaaactg
      421 tacttaatgg cttatttcgc gctgattatg agaatatcag tctgtttgat taatttttag
      481 ggttcaaaat acagagacaa actccaggtc gggtttcgcc acaagcgaat tacccgatct
      541 ggagttttgt ctattgcagg aaggcgtagc cggactgcag gaataaagcc cccggttcta
      601 acagggggct aacaaagaac aggaaactgt gacaactacc cattggggag tgtcctagca
      661 gtattttctt tgtcacgtgc ttatcaattt tgtcacctca ttttgatgat ggatacgctg
      721 catttaacag gcgttctttc tcgacttcca tcacgaatgg tagataaaga tattgctgtt
      781 ctagtagata ttgattatcc tctgtgtact gataagaagc ggtttcttct gaatagacca
      841 acggggcaat tcgcacatcg ctggtcaatt gattaattga gtatagaagg atggctttct
      901 cacctagttc cttgggtaaa tgtttcccaa aataggaata ggatttttga tttgctaaat
      961 agacgagtat gcgatccaca ctaaagttgg agaaggtaaa gggcatcatg tgcagaatat
     1021 gattttgatt atcatgttgc aaatagagct gggcttcctt accaaaatta gttgaaccaa
     1081 gataaagctg ctttttttca tcgatacatt ggaaagtaaa ctggtcttgt tcagttggtg
     1141 gaatgagatt tgtaataaaa aattcaatca ttcgatccat ttctttttca ctaacaaggt
     1201 cagcgtgttt gatttcgtct actatgagac cttcaatctt aggatcaagc ttacctgcct
     1261 ttttttggag catttctaaa atgtattgat tctcttgatc accatacaaa agatattcat
     1321 tggtgacatg acctatctct gcgatttggt gaagggtttc ggtttttgga aggttgatcc
     1381 cacgttccca gttattgact gtacttcgcg gtaaatcacc aattagtttg ccaaatttct
     1441 ccatactcaa ctttaattct tggcgaatct gccgaattcg attgccgact gcttgcttgt
     1501 ctatctccat gaaaactcct cctcaggata agttcttaaa tttagtatac gtgtgtactt
     1561 aaaaaaagtc aaaacatatt gacttaaaat aaatacaggc gtacaataaa aatgtactta
     1621 aaataagttc aataggttct ttgacaagta aatagcagaa ggaggtttgt cgatgaacca
     1681 agttaatcaa catgatctcg gtgaagccat tcgagtttct cgagaagaaa gggggtggac
     1741 acaacgatat ttagcagaaa aagtggggat ttcgagatag ttattatcga aagtagaaaa
     1801 aggaacgaga caattaagtg aagaaaaatt gaatcttgtg ttggatagtt tgcaagaaga
     1861 ggtcgttcca gttaaccgtg ttttgattga ttatctaacc attcactttt tctcgaatca
     1921 acatttaaag ctaattgaag agattattga aatgcccatt gaacgttttg aggaactgga
     1981 ttatgcgcca aagggctata ttggccaata cgtgtggaat caagtaatca cgattcggta
     2041 ttccattgac gatgcggtta aaggaactgt gatggagttt tcaggtcaag gctgtaaaca
     2101 cttagcaaga tggctaaaaa cacgaaaaac aaattggcaa cagttttttc gtaaggtatt
     2161 agattatcag gggaacttta cacggattga ttttacttta gatgattttg tggggagtct
     2221 cagcattcca gaacttaagc ataaggtaat actaggccat gtgtggacca cttttcaagt
     2281 gagtgaatct catggcggta cggatatcat aaacaatgaa tcaaatggtg agaccttgta
     2341 tttaggctca aagaagagtc agtgtcgttt ttgcttttat cagaaggacc atgaacaacg
     2401 gaagcgacga ggaattcctt tagaagaggc aaaagtaaag aatcgctttg aactacggta
     2461 tcgaaaagag aaagcgcaaa gcttggcaaa gattatttca aaaacccatg atttaaccaa
     2521 actgttcttt gagttactta atggagcaat ttgtttttat gaccgtgatc caaatgatct
     2581 aggtgcaaaa gttgatgcca aatgggcagc ctttattagc aattatggcg caatcaccat
     2641 ttctttagat acaattcctc aaagttttga gaaaactatg agttggttaa ttcatggtgt
     2701 ttcaccatca ttagttttta ttgcacaggt cgatcaaacg tttcattctt ccttgttgcc
     2761 tttgattatt gaacaaggag aaatgaatcc aagacagatc aaacttctcc aaaatatgaa
     2821 ggcagacccg gagtattatc aggaggaggt tgagtattat gaacgaaaat tacaaagtat
     2881 atcaaatgag aaaacaagct actaaaaaag caaagtactg cttacacaac aagttgtgca
     2941 acgtactccg cctcgacaag acgagtataa cacaaccatc gaatgagaaa caatagatta
     3001 taaagaggat tattttaaaa ggagtaaaga atcaggaggt gagagaatgg aaacaaaatg
     3061 tgatttcatg gtaaatcgtg caattttgat agagatgggc tttaaaccta gtcaagctgc
     3121 acgaatgatt aaagagagca aggcatatct tgcaaggatt gaaggtattg atttttataa
     3181 taatcgccaa gttggtgtgg tgccatcccg agtaattgaa catctttttc acattcaagt
     3241 tgcggaataa acagtacaag cgcggtacta ttaaagtagc ttttctcaag tacagggagg
     3301 aatggaacga tgccaaaaat taaaaatatt taccaagata aaaagacaaa aaaatggttc
     3361 ttccgagcgt atctaggtat agatgaaaat ggaagaaagg ttcagaagac taaacgaggt
     3421 tatgcttcac aaaaagatgc caaaattgct tatgacaagt acatggaaac acatgatttt
     3481 aacaagacaa ttcctaatca actgtgttct accaatcaaa tgactttcga agagttttac
     3541 aaggataggt ttgtaaaatg gtacgagaga caggtcaaaa gtcaaacata tgagaatgcg
     3601 caattcattt tcgaaaagcg tatgcagttt ttctatcatt ttcgtgttcg agatattaca
     3661 agtcacgata ttgaggattg gttatttgaa ctaagccaga cagagtcacg taattcacgg
     3721 aaaaaagtta atggcgaaac gttatcaaaa tcttatatca atcggattcg agggcaccta
     3781 aaaattgtat tggatcgggc tgttaaagag ggggtgattg ctaaaaatcc cgttgaagat
     3841 gtttcttcgc ttccttctga gaataagaaa gttgagtttt gggaacacag tgagtttcaa
     3901 aaagtgatga atgaattgaa ggatgataag attcaaaccc accatcggaa gattgtgtat
     3961 gaaatgctct tttatactgg cattcggatt ggagaacttg aagctttgtc ttggtcgaat
     4021 gtagattttg agaaaaacac gataacgatt gagaagacat tgatttataa tacaaaagat
     4081 gattggtact tttcgactcc caaaactaaa tgtgcttatc gaacgattgg gttggggaag
     4141 aagctttcct taaaactcag acagtggtat gacttgcaaa aacgtattgg aaattttgaa
     4201 tatgttgctc agttggacgg aacatttacg ccaccttatt cttttgcgaa ctggttaaaa
     4261 gaagctgcta aaaaagcggg tgtcaaaccg attaagcttc acgcgctacg acattctcat
     4321 gttgcatttc taattgagca gaatattcaa ccgctatcta ttcaagaacg attgggccat
     4381 gcgaatattc aaatcacttt aggaacttac ggtcacttat atgcaaagtc agatcaacaa
     4441 gtggtaagtg cgattgataa tgttcaagag ggcgctattc tttcaaaaaa tgacaattta
     4501 ttaccagtct gagttttcca cggttctacc ataagacaaa taaatgcttt tgcaccagtg
     4561 ttttcagtcg aattgctcct gttcaagatg tatcggaaat agcgagaaaa taagcaaatt
     4621 tgcataacta taataaaaag gacttagttt tgatgggaaa tttttcaaaa ttaagtcctt
     4681 ttttatgtgg ttgaaacggg tatgatcatt ttaaggaagt gatctaagat gaaaggacga
     4741 ctgaacaatt
//
LOCUS       DACEPW010000168         4744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEPW010000168 DACEPW010000000
VERSION     DACEPW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.172"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            690..2552
                     /gene="ltrA"
                     /locus_tag="IW819_002289"
     CDS             690..2552
                     /gene="ltrA"
                     /locus_tag="IW819_002289"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1245378.1"
                     /translation="MENVSASHSIRHMEYYGMIDSFDNLYKESRDDHIFNNLMEIVTS
                     PNNILLAYRNIKGNSGSVSPGVDNKNIDDLKEIPNADFIRIVQGKFSEYKPQPVKRVE
                     IPKPNGKNRPLGIPTIWDRIVQQCLLQVLEPIMEAKLHDKNYGFRPNRSAHHALAQAV
                     RLAQLSKLTFVVDIDIEGFFDNVNHSKLIKQLWTLGIQDRWLLGVIRAMLKAPIIHKD
                     GRIEHPKKGTPQGGILSPLLANVVLNELDWWISSQWETHPTRHNYDCYQANQGYWNKG
                     NKYRALRRSKLKEIYIVRYADDFKIFCRRRSDADNIFIATKLWLKERLKLDISLEKSK
                     VVNLKKKKSEFLGFTMKLVRKRKTFVIETHMCPKAMKKVSDNLTKQIKVIQHTPTKED
                     LQKQLVKYNSMVIGIHQYYRRATHISVDCPKINQRIRIIIKNRLDVKKNGSPPSDFVK
                     KNYARSKQLRWINGYPMIPLGFVKHSHTSLPKMGICKYTPEGRVLIHKYLTFPIDIIN
                     QLMNMNNPDESIEFRDNRVSKYSAQRGRCRVTKELLELEDIYCHRIIPKNLGGKDNFQ
                     NLVIIHQNIHQLIHENDRTRASLLITRMRITGAQLEIINQFRKKAQKCELSLNL"
     gene            2634..3928
                     /locus_tag="IW819_002290"
                     /pseudo
     CDS             2634..3928
                     /locus_tag="IW819_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            3921..4268
                     /locus_tag="IW819_002291"
     CDS             3921..4268
                     /locus_tag="IW819_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245379.1"
                     /translation="MIEYNDRGRLKWVGFYLSDHTERINSNNVQRANHNSAKEQMTTE
                     EIAKVLKTAVIKNRPVCIQKEERNAEGYYPDDIVGKIIGSDELGVFVEEEKVHYDEIR
                     NVSFTELVKWSQI"
BASE COUNT         1728 a          778 c          958 g         1280 t
ORIGIN      
        1 aaaaggttag gcacacaaga ctttcttgtg tagaactaat gatttgagga gtgaaatgac
       61 ggtgtaacga cctgaagcgc tccctctgat actccgattg gcaatacctt tagttagaat
      121 aggaattgtc aaaagctcgg taaagtcggc tgagagttac cgtagttgca ttatgcaatc
      181 gaaagctgtt cagcggtgaa tggtgtaacc tataggtcgg gagtccagaa attactcatg
      241 gtgagaatgt gcagttgcac tgacgaactc gcgaatgtac gaatctataa gcttggggtg
      301 cagaaatgta tcttacccgc acaaatatta ctgtgacaaa gggtgtagct tgcggttaag
      361 taagaatcgt agatatgaac aaccgtgcaa tgtggtagac attggaaagt tacaggttca
      421 gagtgagcac ctaaaagtaa atgcagggat aaaatcattg gaacgttgaa agctagtgtc
      481 gtggaggact aacttccttt gaaacggttg agacgaagaa taataagctc atttacacta
      541 gtgaaagtag tggcacagta cctatgaagc agtgataata aactgtagag ggatagccac
      601 aagtcaattt actctactaa cagtaatgat ataatccata gaatcgagta tgacaaaata
      661 aacgccgacg tccgaaagga ggcggtgcta tggaaaacgt atcagcttca cattcaatcc
      721 gtcatatgga atactatggc atgattgaca gctttgataa tctatacaaa gaaagtcgcg
      781 atgatcatat tttcaataac cttatggaaa ttgtaacctc gccaaataat atcttactag
      841 cgtatagaaa tattaaagga aattctggga gtgtatcccc aggcgttgat aacaaaaata
      901 ttgatgattt aaaagaaata cctaatgcgg attttatcag aatcgtgcaa ggcaaatttt
      961 cagaatataa accacaacca gttaaaagag ttgaaatccc aaagccaaac gggaaaaatc
     1021 gcccactcgg catcccaact atttgggata gaatcgtaca acagtgtcta ctacaagtcc
     1081 tagaacccat tatggaagca aaattacatg ataaaaatta tggttttcgt cctaaccggt
     1141 cagcacacca tgcacttgct caagcagtac gcttagcaca attgtctaaa cttacttttg
     1201 tagtagatat agatattgag ggattctttg ataatgtaaa ccattcaaaa ctcattaaac
     1261 agctatggac attaggaatt caggacagat ggcttttagg tgttattagg gcaatgctta
     1321 aagcaccgat catccataag gatgggcgaa tcgaacaccc taagaaagga actccgcagg
     1381 gcggaattct ttcccctctt ctcgcaaatg ttgtgttaaa tgaattagac tggtggatat
     1441 catcacagtg ggaaacccac cccacaagac ataattacga ttgctatcaa gctaatcagg
     1501 ggtattggaa taagggtaac aaatatcgag cgttacgtag atcaaagtta aaagagatct
     1561 atattgtgag atacgccgat gacttcaaaa ttttctgtag aagacgtagc gatgcggaca
     1621 atatatttat agctaccaaa ttatggctaa aggagcgcct gaagctcgat attagcctag
     1681 aaaaatcaaa agtggttaat ctgaagaaaa agaaatccga attccttggt ttcactatga
     1741 aattggttcg taaaagaaag acctttgtca tagaaactca catgtgccct aaagctatga
     1801 aaaaagtatc tgataatcta acaaaacaaa taaaggtaat tcagcacacc cctaccaaag
     1861 aggatttaca aaaacaacta gtgaaataca actcgatggt gatagggatt catcaatact
     1921 atagaagggc tacccacatt agtgtggatt gcccaaaaat taaccaacgt ataaggatta
     1981 tcatcaaaaa tcgattggac gttaagaaaa acggatcacc gcctagtgat ttcgttaaaa
     2041 aaaactatgc acgttcaaaa cagttaagat ggattaatgg ctatcctatg attcctctag
     2101 gattcgttaa acacagccat acatcgttac ctaaaatggg gatttgtaag tacacgcccg
     2161 aaggacgtgt gctaatacac aaatatctta ccttcccaat cgatattatc aaccaactta
     2221 tgaatatgaa taacccggat gaaagcattg aatttaggga taatcgtgtc tctaagtact
     2281 ctgcacaacg aggcaggtgc cgagtaacaa aagagctctt ggagctagaa gatatttatt
     2341 gtcaccgtat catacccaaa aatcttggtg gaaaagataa cttccaaaat ctagttatca
     2401 ttcatcaaaa cattcatcaa cttattcatg agaatgatag aacgagagct tcactcctca
     2461 taacgaggat gaggattaca ggcgctcaac ttgagataat caaccaattc cggaaaaaag
     2521 ctcagaagtg tgaattgtcg ctgaatctat aactgtatga taattgttag taaacattga
     2581 tggaacgccg tatgcggtga aagtcgcacg tacggtgtgg agcgggggaa aatctggaga
     2641 ttatatcaaa ggattaccta tcgctatcta gcgtggaatg tattgaaaga aatttagacc
     2701 caatgactac accgttaatt gtcatgagta gatcggacaa cactggttca ggtctgactc
     2761 tagcttcttc acctactgca aagaaaatat atcatattac taatgttagt agagctactg
     2821 atttacctta tccattacca aaagaacttc atattgttcc accacgaatg aacttatata
     2881 taaaaaagaa tatggaagtt aatcagattt ttagaaggta tgtagcagac gaagatttat
     2941 tgatatattc gatcgatgaa tcgatcctaa aagttactcg atcactgaat ctttttacga
     3001 ctgaaggaac aagaagtcag cgaagaaaaa aactcgctca aatgatccaa ggacacatta
     3061 aagaagagac tggattaatc gctacggtag gtgtcggcga taatcccctt ttggctaaac
     3121 tagccttaga caatgaagca aagcataatg aaggatttgt tgcggaatgg acgtacgaaa
     3181 acgttcctga aaaagtatgg aatatcaaag aaatgacgga tttttggggt attggatcac
     3241 gcatgaaaaa aagattgaat cacatgggaa ttatgagtat agaagattta gcaaattgga
     3301 atccctacac aattaaaagt cagttaggtg taatcggact tcagttatat ttccatgcca
     3361 atggaattga tcgtacggat attgctattc caccagaacc aaccaaagaa aagtcttatg
     3421 ggaattcaca agttctacca aaagattata cgaggagaga agaagtagaa ctagtagtca
     3481 aggagatggc agagcaagta gccattcgaa ttagacaaca taattgcaaa actggttgtg
     3541 tacgcttagc aattggtacg tcaattttgg aagaaaatca ggtttctcgc atcagatgaa
     3601 aataccaatt acagataata cgaaagaatt acaaaattat tgtctttttt tattcaataa
     3661 gttttatgag gggcaggaag tacgacatat aggagtaact tatagcagat tagtatacac
     3721 agattcccta caattagatt tatttagtga tccaagaaaa aagattgctg aagaaaattt
     3781 ggataggatt attgataaga ttagacaaaa atatggattt acagctatta tacgtgcttc
     3841 atctatgtta gaaggtgcaa gaagtattaa gagaagcact ttaataggag gacacgcagg
     3901 aggattagga ggtttgcata atgattgaat acaacgatag aggacgttta aaatgggtag
     3961 gattttattt gtctgatcat actgaaagaa tcaattcaaa caatgtacaa cgtgccaatc
     4021 ataactcggc aaaagaacaa atgacgactg aagagattgc gaaagtactg aagactgctg
     4081 tcataaaaaa tagacccgtc tgcattcaaa aagaggaacg aaatgctgaa ggttactatc
     4141 cagatgatat agtaggaaaa attattggaa gtgatgagct tggtgtattt gtggaagaag
     4201 aaaaagtcca ctatgatgaa attaggaatg tttcttttac ggaattagta aaatggagtc
     4261 agatatgaac attattaaga aatacgaatt gggttatgtt acctatgaag agctaataga
     4321 ggaaatatgg ggatatggtc aacaattaat taatcaagtg gggattgatt gcttttgttt
     4381 ctatattgaa tccggagcag gataccataa gaacagatac tatatatttc catatccacc
     4441 aaaatagtct cttagagatt tttaaatttt ctccaaatac tttcaattat tgtgaaatat
     4501 ttcccttaaa acaaatagta aaagctatga atcagctcag aaagcattaa aatctgaaga
     4561 agagcaattt tttacagaag aggaattgga taagatgtta ccagagcatc ttagaaaaaa
     4621 gagttctaaa aattgaactc tttttttatg cttaaaattc tcttgtaatt tttctattca
     4681 atcaataaat aatctatatt taagaagaaa tatgataata gaacatctcc taaagccacc
     4741 gcaa
//
LOCUS       DACEPW010000169         4736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEPW010000169 DACEPW010000000
VERSION     DACEPW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.173"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..1518
                     /locus_tag="IW819_002292"
     CDS             91..1518
                     /locus_tag="IW819_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1245380.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1610..1975)
                     /locus_tag="IW819_002293"
     CDS             complement(1610..1975)
                     /locus_tag="IW819_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1245381.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1972..2337)
                     /locus_tag="IW819_002294"
     CDS             complement(1972..2337)
                     /locus_tag="IW819_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1245382.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2529..2777)
                     /locus_tag="IW819_002295"
     CDS             complement(2529..2777)
                     /locus_tag="IW819_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245383.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2776..2904
                     /locus_tag="IW819_002296"
     CDS             2776..2904
                     /locus_tag="IW819_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1245384.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2961..4655
                     /locus_tag="IW819_002297"
     CDS             2961..4655
                     /locus_tag="IW819_002297"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1245385.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
BASE COUNT         1562 a          900 c          837 g         1437 t
ORIGIN      
        1 tttttcagaa attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg
       61 tgcaaaaaaa tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt
      121 ttaagacagc tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag
      181 ctctccaatc tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa
      241 aaagaatttg aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat
      301 ttttcagaac atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt
      361 ttaaaaactt gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa
      421 tgtgaattca tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttacttt
      481 aatgactgcg ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt
      541 ctgctgcttc ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa
      601 tcgttttttg aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt
      661 tatgataaag aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag
      721 caagtgtgtg ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt
      781 tacaattatg tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa
      841 gaattcgcct ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag
      901 ttgattgcgg aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata
      961 aaagaactag ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg
     1021 ttcgaacgtg cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg
     1081 ccagaaaaat tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca
     1141 attttttcca gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg
     1201 cgggcttttg tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc
     1261 tatatcgtca caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta
     1321 actactttcg attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag
     1381 tatgcgcagc agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg
     1441 gtccaatatg aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca
     1501 gctgtgcaaa acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga
     1561 agatgcccat ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt
     1621 ggacatctag gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta
     1681 ccaacgccgc tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc
     1741 cagctgaaac aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag
     1801 cattgttctc gcttactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa
     1861 aatccttgat tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc
     1921 ccagtacaag tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac
     1981 ctctcctctt ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac
     2041 tgttcgaatg ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac
     2101 tgactcgatg atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag
     2161 cagttttctt gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga
     2221 ccaatcaaaa agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca
     2281 aatatttaaa atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata
     2341 cgctacctac tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt
     2401 tcgtctattt aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct
     2461 cccatttttc tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa
     2521 aataaggctt agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta
     2581 cccaaatttc taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta
     2641 tttttattta caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct
     2701 cgtcctatca tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac
     2761 aaggaggcgt ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt
     2821 gcccttggcg ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta
     2881 tatgatgaat tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg
     2941 aagacattgg aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg
     3001 ccaatatggc agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca
     3061 ccttttattc attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg
     3121 atgaatattg gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg
     3181 gtcgcatgct taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt
     3241 ctgttactga gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc
     3301 gaacctttga caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac
     3361 tgggattcaa taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag
     3421 aagaagaaaa actagacaat atcactatcg aagaatattt caaagatgat ccacactttt
     3481 ttgaaacaaa cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg
     3541 ctcaagaatt gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc
     3601 tagtgggcgt caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg
     3661 agtacttgaa agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat
     3721 tcaaagaaac accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac
     3781 tgactcaaga agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg
     3841 ggtccattac agattcagct actttaggag attacgaaac accagctgta gaaaatatgg
     3901 attatggtgc agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa
     3961 atcctgacaa attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga
     4021 cgaaaaacca tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg
     4081 ccttgaattc atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata
     4141 tgtctattgt cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta
     4201 tttggggtta tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta
     4261 aaatgaatgg aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg
     4321 gaccagtgaa tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc
     4381 ctgtctatat gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa
     4441 aagtcattcc taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt
     4501 atcagatgat ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt
     4561 ttgcgggtat tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta
     4621 ctctagctcg tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc
     4681 tgcatcaagt aataagaaca accaggctaa aacagcttct tatgtttttg atttgg
//
LOCUS       DACEPW010000170         4702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEPW010000170 DACEPW010000000
VERSION     DACEPW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.175"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            81..515
                     /locus_tag="IW819_002298"
     CDS             81..515
                     /locus_tag="IW819_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1245386.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(593..1126)
                     /locus_tag="IW819_002299"
     CDS             complement(593..1126)
                     /locus_tag="IW819_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1245387.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1220..2398)
                     /gene="mutY"
                     /locus_tag="IW819_002300"
     CDS             complement(1220..2398)
                     /gene="mutY"
                     /locus_tag="IW819_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1245388.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2391..3188)
                     /gene="recX"
                     /locus_tag="IW819_002301"
     CDS             complement(2391..3188)
                     /gene="recX"
                     /locus_tag="IW819_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1245389.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3295..4677
                     /gene="rlmD"
                     /locus_tag="IW819_002302"
     CDS             3295..4677
                     /gene="rlmD"
                     /locus_tag="IW819_002302"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1245390.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
BASE COUNT         1373 a          960 c          859 g         1510 t
ORIGIN      
        1 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
       61 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
      121 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
      181 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
      241 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
      301 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
      361 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
      421 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
      481 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
      541 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
      601 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
      661 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
      721 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
      781 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
      841 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
      901 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
      961 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     1021 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     1081 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     1141 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     1201 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     1261 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     1321 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     1381 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     1441 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     1501 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     1561 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     1621 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     1681 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     1741 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     1801 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     1861 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     1921 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     1981 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     2041 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     2101 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     2161 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     2221 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     2281 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     2341 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     2401 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     2461 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     2521 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     2581 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     2641 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     2701 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     2761 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     2821 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     2881 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     2941 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     3001 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     3061 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     3121 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     3181 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     3241 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     3301 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     3361 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     3421 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     3481 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     3541 ggtggctgtc aattacaaca cttagcttat caagctcaat tggattttaa aaaggatctg
     3601 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     3661 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     3721 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     3781 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     3841 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     3901 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     3961 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     4021 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     4081 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     4141 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     4201 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     4261 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     4321 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     4381 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     4441 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     4501 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     4561 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     4621 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     4681 aaagaggttg tgaaaaagtc tg
//
LOCUS       DACEPW010000171         4689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEPW010000171 DACEPW010000000
VERSION     DACEPW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.176"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            315..2159
                     /locus_tag="IW819_002303"
     CDS             315..2159
                     /locus_tag="IW819_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1245391.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSSTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2214..3680
                     /locus_tag="IW819_002304"
     CDS             2214..3680
                     /locus_tag="IW819_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1245392.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3717..4559
                     /locus_tag="IW819_002305"
     CDS             3717..4559
                     /locus_tag="IW819_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1245393.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1525 a          756 c         1006 g         1402 t
ORIGIN      
        1 gttggaagat attcttagaa aaaagacaaa acttttagaa cacgaaaact atattgaacg
       61 aataaaatga aaaaatgatt aaaaaatcat tttattattt acgtttacat tctaatatgt
      121 taaattgaat ataacttaaa caggcatgtg actggtaatg caggcaggac ctaaataagt
      181 tgaataacaa tgtatgcgtt tcaatacacg tttgccttgt tgtcaattta tttagtttct
      241 gcctgttttt tgtgttgagg atggccactt cgactcaaaa gttcatcact caaaaaagaa
      301 aagagggaaa caccatgaat tacaaacaaa cagcagaaga agtattgcag tacatcggtg
      361 gggaagaaaa catatcccat ttggagcatt gctcaactcg tttacgtttt acgttaaaag
      421 acaacagcaa ggtaaatatg agccagttag aaaaagtgga tggcgttatg ggcgttcgcc
      481 aaaatgtgca atgccaagta atcattggaa acgatgttgt tgaagtgtat gatgaagtga
      541 aagcgttatt aggtgacaat gtttcaagta ctgaagaagc accaaaacaa aaacaaaaat
      601 taggttcagt tctattagat tttattattt ctattttcca acctttaatt cctgcgattg
      661 caggtggagg agtattgaag tccttgttgc tactagcttc tatcttaggg ctgattagtg
      721 accaatctca aacctatcag attttaaatt tggtcggcgg ggcaccgctt tatttcttgc
      781 ctattctggt ggcaatgact acggctaata aattgaaagt caatcaattg gttgcagtat
      841 ctgctgtcgg tgcattgatc cttcctgaaa tgacagctat gttggcggaa ggtgctacat
      901 tcttatcttt tggattggat aatatagcgt atgcatctca agtattccca gccatcttaa
      961 ctgttttatt ttatgctcaa cttgaaaaat ggtttacaaa agtttcacca aaaccaattc
     1021 gtattttctt tgtacctatg atgagtttat tgattacagt tcctattgct ttgttgcttc
     1081 taggaccatt aggttataat gtaggaacag ctttctcttc tatcattgtg tctttgtaca
     1141 atcaatttgg atgggttgca accggagtct tagcagctgt actgccatta atggttgtaa
     1201 cgggtatgca taaagcaatg atcccttacg ctgtttcatc catgagtgaa ttgggtgcag
     1261 aacttcttta tctcccggct tcattggcac acaatatttc agaagctggc gcatgctttg
     1321 ctgtcagtat caaaacaaaa gacaaaaaat tgaaatctac ggctgtttct gcgggtatat
     1381 ctgcattatt cggaattact gaaccagctc tttacggggt aacgattctg cacaaacgtg
     1441 ttttatatgc cgttatgttt tctagcctga tcgggggagc atttgcagga atcgttgcac
     1501 ttcaagcctt tgctttagtg ggtcctggga ttgcaagtat cacgatgttc gctgacaaag
     1561 ctgacccaat gaatctggtt tgggcatttg cagtatcggg gatttcattt gtggttgctt
     1621 ttgctgcagc gttggttctt tataaagata aagaagttgt caatgaagtt gaaactaaga
     1681 tggaaacggc cgttgtttca gatgctgttc tttcaagtcc cgtgaatgga aaagtaatct
     1741 cactatcaga agtaaaagac actgtttttt ctagtggtat ggtaggtgat ggagtagcaa
     1801 tcattcctga agaagggata ttagtagctc ctgaagatgg agaaatcaca atggtatttg
     1861 aaacgaagca tgctattggt ttaaaaacgg ctaatggtgc agaacttctt tttcatattg
     1921 gtattgatac tgttcagtta gaaggaaaag ggtttgaggc atttgttcgt tctggtgaaa
     1981 aagtagccaa aggacaaaag ctgattgcat ttgatattga tttaatcaaa gaagcaggat
     2041 tcgaccccac agttatctgt gtggtaacca ataaggataa ttacactatc cgttcattta
     2101 acctagaaaa attggtaacc aatgatagtg atctcttagc tatcagtccg atagcttaaa
     2161 cacaagatag tgaaaaaaat atattgaatg aaggaataga ggagaataga ataatgacaa
     2221 acacaacaag ttttccaaaa ggatttttat ggggcggcgc gatagcagca aatcaagtag
     2281 aaggtgcttg gaatgtagac ggtaaaggtc tttcagtagc ggatatggct acttataaac
     2341 caaatttaga caacaaagat tttgctgctc atatgcatgt caccctgcag tctataaaag
     2401 aagctagtga agacaaatct gataaatggt acccaaaaag aaggggcgtt gatttttatc
     2461 atcactataa agaagattta gctttatttg ccgaaatggg ctttaaaacc ttgcgcctct
     2521 ctattgcttg gactcgcttg ttccctacag gagaagagct ggaaccgaat gaaaaagggg
     2581 ttgcatttta tgaaaacgtc tttaaagaaa tgcaacgttt aggaattgag ccgattgtga
     2641 cactatctca ttatgaaatg ccattagctt tagcagttaa atataatggc tgggtggagc
     2701 gtcgagtaat tgatgatttt gttcgcttcg ctaacgtttg ttttgatcgc ttcggcaaat
     2761 acgtaaaata ttggctgaca tttaatgaga tagacagcat ccaccgtcat ccatttacaa
     2821 cagctggaat tatccaagag aaaagcgcag aaggtcaaga agctcaagat atctatcaag
     2881 cattgcatca tcaatttgtt gcgagtgcgt tagtgacaaa agatgctcat gaaaaaattc
     2941 caggaagcca agttggttgt atgttaacga aattgatgac ttatccatta acttgcgcac
     3001 cagaagacgt tgagctgacc ttgaaaaaga acttagaaaa taatttttat gcagatgttc
     3061 aagttaaggg agaatatcct gctctaatcg aaaaggattt ggaaaatcgg ggaattcaga
     3121 ttaaaatgga agatgaagat cgagaaattt taaagaaaca taccgtagat tttgtttctt
     3181 tcagctatta catgtccatg gcagaatcag ctgatccaaa cgcagaacgc acaccgggaa
     3241 atactgtttt aggggttaag aatcctcatt tgccttcaac agattggggc tggcaaatcg
     3301 atccaaaagg tttgaaaatt tcgttgatcg aactttatga tcgttaccaa gtaccgttga
     3361 tgattgtgga aaatggtatg ggagcagtcg atcaggtagt agatggtaaa atccatgatc
     3421 cgtaccgcat cgactacttt aaagcacact tccaacaaat gaaagaagca atcgatgaag
     3481 gtgtagattt gatagcttat actagctggg gaccaattga tttagttagt gcaggaactt
     3541 ctcaaatgtc taaacgatat ggatttattt atgtggatgc agacgatgaa ggaaatggga
     3601 catataaccg cttgaaaaag gattcgttca actggtacaa agaagtgatc gaaacaaacg
     3661 gtgcatcctt attcgagtag tctcgatctt tttaagcagc gataggggga taatgagtga
     3721 tttcaataaa aaaagtattg aattcaagtg tggtactggt tgaagaaaat ggcaaagaaa
     3781 tgattgcatt aggtaaaggg ataggctttg gacagaaagc tggggatgaa atatcaggag
     3841 ataaagtaga taaaatattt ctgccttttg aagaaaagaa atcttcacaa ctattagagt
     3901 tagttgaaga aatacctatt gaattttttg aaatcactcg tactatcgtt tttgaagcag
     3961 aaaaaggatt agataaaaaa ctgaatcctg gagtctattt aaccttaaca gaccacctgc
     4021 attttgcagt tgaacgcgca caaaaagggg taaatattgc aaaccgtttg tactgggaaa
     4081 taaaaatcta ctatcccaaa gaatttcaaa ttgggaagta cgcactcacc cttttgaacg
     4141 agcaatatgg aattgatctt cctaaagagg aagcctcaaa cattgctttc catttgatca
     4201 atgcacaatc ggaagatcct gatagcggcg ctggattcaa gtatgcaaaa atgatcggcg
     4261 ggatcgtcaa tttggtccgg tattcgttgc agaaagatat cgatactaat tcgatccatt
     4321 atgcccgctt cattacacat gtacgtttct ttgtagaaag atattattct gatgctttgt
     4381 tggatgatgg tgaggaagaa ttctacaatc aaatgtggat cttatacccg tctgccatgg
     4441 aaagcgcagc taagatcaaa gactacattg aaaaagttta tcataaaaca attccgaacg
     4501 atgaagttgt atacctaggt gtccacatca atcggttgat gaaacatgct aatgggtgag
     4561 cgctcagtaa tactattgat gcttttaaaa tctttagaga tggattatag taaaaacaag
     4621 gtgatttcca gctgtttgtt atctataaga gacatttaaa aaggattaac gtaagataat
     4681 aaaaaatct
//
LOCUS       DACEPW010000172         4687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEPW010000172 DACEPW010000000
VERSION     DACEPW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.177"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..1055)
                     /locus_tag="IW819_002306"
     CDS             complement(93..1055)
                     /locus_tag="IW819_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1245394.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1057..2496)
                     /locus_tag="IW819_002307"
     CDS             complement(1057..2496)
                     /locus_tag="IW819_002307"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1245395.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2681..4627
                     /locus_tag="IW819_002308"
     CDS             2681..4627
                     /locus_tag="IW819_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1245396.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1489 a          801 c          770 g         1627 t
ORIGIN      
        1 gttgacaact cctttttgtt tacaaatcaa ttggatatct acaaattgta ttttctaacg
       61 aattttatag acattaaaaa attaatttaa cgttaaatac ttcctccaga aagtattgag
      121 attggtaaaa tataatcctt atttgtcttt tcgcctttaa tttttttcag aagaatatca
      181 acaataagac ttgcaatttc atcaattggt tgtcttattg ttgtcaattg tgggaaaaat
      241 ttctcaataa aagaggtacc atcgtatcca ataattttta aatcttctgg gatattaagt
      301 ttaagttgat gagcaatttt catagtgaga atggctgtta aatcatcaga aacaaaaata
      361 ccatctggtt tgtgatttat aatagttgac tttatttcca tttcacgacg gatagtagac
      421 agattattag gaattttgaa aatttttcca tttggatttt gaaaagaaaa acctagtgca
      481 cgtaattctg tgggagaatc cgtgttatca tttcctgtaa tcataatggt attttgacaa
      541 ccattttttt gtaaagtctt agctgcaagt tttccccctt caaagttatc tgaagaaaca
      601 atggaaacat taggagctaa atttctatca aaagcaacaa taggtgctct cacacgctca
      661 taatcagcaa ttccaagatt atggcttgat gagattatgc catcaacttg gttggcttca
      721 agcatttcaa tgtattctct ctctttagca ggatcatttt gactattgca aataatcgtt
      781 ttatatccat gtttaaataa ctctatttcg agatactcaa tgagttcaga gtaaaagata
      841 ttactaatat taggaaaaat aagaccaata agctgaggtg actttccttg aagtccacgt
      901 gctaaattat ttggtttata accaagagtt ttcatagctt catgaacttt ttgttttgtt
      961 ttttctgata aatatccctt attattaatg acgcgtgaaa cggttgttgg gctaactcca
     1021 gctaattcag cgacatcagt taattttgct accattttaa tattttaatt caaagtattt
     1081 tccagtaggt tcgcctgatt tgatttgaat accattttgg tgattttcag ggaatacacg
     1141 actagtaaac actttttctc ctttattaat aaaaatctca atgattgagt tatctacaaa
     1201 aatgttagca gttgtttctt tagtatcaat tgagcattca cgaatcgtac caaagtcagt
     1261 ggcatattga atgcctgctt tgctgcgatc taaaataatt tttccttctt tggtatctac
     1321 aatcaagcta agtccatttc ctttactatc agagaaaaga agtaactccg atttctgatt
     1381 tggaggaaaa gttagttcaa gctcataagt attatttgtt tctgcttttg ctgtaaattg
     1441 ttcagaacta gcacgtaatg atgtaatagc ttctactgga tattggtaaa gtttaccatc
     1501 ttttattgaa agttctttta caagactcat tgctccctga taatcatact tatcagttgg
     1561 ataatcgata tcaggtagtc caatccaact gacaatcaga gcacgaccat caggagtatt
     1621 gaatccttga gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc
     1681 cacaagtttt actttttcta tgtcaagcga ttgacagatc ttaaaagtat tcggatagat
     1741 attcccataa tttagctcag atttatccaa cccttgagga caataaatga gaacaggttt
     1801 tttgtctaca aatactagat ttggacactc aatcatgtat tctgaaccag tgccgccaaa
     1861 atcaaggttg ccaacttcct cccatgtttc cacgttattg tcatccgctt tgtaaagttt
     1921 gataaagcct gatttttcta aattttgtgc tccaacaatc gcatacaatt gtccttcata
     1981 attaaacatt tgtggatcac gaaaatgatc agttacatca gcaggtttat gaataagaac
     2041 attctcacct ttcgtaatgt tgtagtcttt atccatccat gctccgattt gaagtgtatc
     2101 acgttcccaa ttttcatttc gaacgtttcc tgtatagagt aagaatagat tcccctctat
     2161 ttcatatgca gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc
     2221 tgctcctgtt acatgaaaat gaactaaatc atctgattca ttgtgtaccc attctttcaa
     2281 cccatgagca gctccaaatg gccaattttg ataaaaaagc gtatattttc cgttaaaaaa
     2341 agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat agctagcacg
     2401 ccaaggtgat cgttttacat tatccttaat tttatccatc tcatctttag tccaatcaga
     2461 ataagcacga tatcgtattt ctcgaggtaa gttcatattt tactccttca atttatcttt
     2521 ttacaactag tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata
     2581 aactatatga caaacgtttg acatattttt aagatatgct aaaataaatt tgcatttcag
     2641 tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga atgaattaca gtaaagtagc
     2701 ttccgacgta atacaagcgg tcggaaaaga taaccttatt gcagctgcac actgcgctac
     2761 acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat caaaaagctt tagacgaaaa
     2821 tccagatgtg aaaagcacat ttaaaattga tggacaatat caagtgatta tcggtgcagg
     2881 tgatgttaat ttcgtttatg acgaacttat caaaaaaact ggactttcgg aaatttcaac
     2941 agatgattta aaacaaattg ctagtaaaga taaaaaattc aaccccatta tggcattaat
     3001 taaattatta tcagatattt ttgttccaat tatacctgcc cttgttgcag gtggtttatt
     3061 aatggcatta caaaactttt taacagctgc tggacttttt ggaccaaaat caattgaaga
     3121 aatgtatcct gcgattgaag gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt
     3181 catgttctta ccaattttag taggtatttc agctgctaaa cgttttggag caaaccaatt
     3241 tcttggtgct gcaattggta tgatcatgac aacaccagat cttggtggct caactgaata
     3301 ttggaatatt tttggttatc atgtttcaca aacaaactat gcttatcaag ttattccagt
     3361 tttagcagct gtttggatat tatctatatt agaaaaattc tttcataaaa aattgccatc
     3421 atctattgac tttacattca cgccattatt atcagtaatg attactggat ttttgacttt
     3481 cacagttatt ggccctgtaa tgttattgtt gtctaatgga attacagaca ttattgtttg
     3541 gctatataac actactggat ttattggaat gggtattttt ggaggaacat attcacttat
     3601 agtcatgact ggtttacatc aatcatttcc agctattgaa actcaattac tttcagcgtg
     3661 gacaaatggt atcggacatg gagactttat ctttgtagtt gcatctatgg caaacgttgc
     3721 tcaaggtgct gcaacatttg ccatttggtt cttaactaaa aattctaaga caaaaagtct
     3781 tgcttcctct gcgggtgtct cagcacttct tggaattaca gagcctgcct tgttcggagt
     3841 taacttaaaa taccgctttc cattcttttg tgctttaatt ggttcaggaa ttgctacagc
     3901 agtagcagga ttacttaaag tagttgctgt ttcacttggt tcagcaggtt tcttgggatt
     3961 tctttcaatt aatgcaacat ctattccatt ttacgtattg tgtgaattga ttagctttgt
     4021 agttgctttt gcaattactt acttctacgg taaaacaaaa gctgctggta tctttaaagc
     4081 agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa cagatccaat
     4141 atctactgat gttgaatcaa cacaaacaaa agtttcatca gaaacaattg ttagtcccat
     4201 ttctggtaaa gtgattagtc tttcttcagt aaatgatcca gtcttttcat cagaagctat
     4261 gggaaaaggg attgccatta aaccaaatgg aaacacggtc cattctccag tagatggtac
     4321 tattcaactc acgtttgaaa ctggacatgc ttatggtatt aaatcaaata atggtgctga
     4381 agttctaatt catattggta ttgatactgt atcaatggga ggaaaaggat ttacacaaaa
     4441 agttactact aatcaaaaag ttagaaaagg cgatattctt ggagcatttg accgttcaat
     4501 gattgagaaa gaaggtcttg atgatacaac aatgattatt gtgaccaata cttcggatta
     4561 ttcagaagta aatatagtag ctgaaaatac agtcactgag ggtactgatt tacttgaaat
     4621 caaataaata tatttccctg agtaaagatg gaattttctt tccaaaatca aataatatag
     4681 attaatc
//
LOCUS       DACEPW010000173         4685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEPW010000173 DACEPW010000000
VERSION     DACEPW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.178"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..223
                     /locus_tag="IW819_002309"
     CDS             <1..223
                     /locus_tag="IW819_002309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245397.1"
                     /translation="LIVLKACKNILVNLSRCNQMDLKTFTAQIELMHQEALRQSASYE
                     DKWLNTFHGGRESALDQVLKLLKGERRDG"
     gene            216..518
                     /locus_tag="IW819_002310"
     CDS             216..518
                     /locus_tag="IW819_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245398.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVTGTAEQLASLTGLHEKIVRKRARCGYTDVKKRTFRYVEGSS"
     gene            515..676
                     /locus_tag="IW819_002311"
     CDS             515..676
                     /locus_tag="IW819_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1245399.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERPKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            673..999
                     /locus_tag="IW819_002312"
     CDS             673..999
                     /locus_tag="IW819_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245400.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            996..1811
                     /locus_tag="IW819_002313"
     CDS             996..1811
                     /locus_tag="IW819_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ1245401.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            1950..2261
                     /locus_tag="IW819_002314"
     CDS             1950..2261
                     /locus_tag="IW819_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245402.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            2258..2488
                     /locus_tag="IW819_002315"
     CDS             2258..2488
                     /locus_tag="IW819_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245403.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            2495..2698
                     /locus_tag="IW819_002316"
     CDS             2495..2698
                     /locus_tag="IW819_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1245404.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            2695..2994
                     /locus_tag="IW819_002317"
     CDS             2695..2994
                     /locus_tag="IW819_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245405.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            3237..3650
                     /locus_tag="IW819_002318"
     CDS             3237..3650
                     /locus_tag="IW819_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1245406.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILVALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            3663..4049
                     /locus_tag="IW819_002319"
     CDS             3663..4049
                     /locus_tag="IW819_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245407.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
BASE COUNT         1676 a          701 c         1133 g         1175 t
ORIGIN      
        1 gttgattgtg ttaaaggctt gcaaaaacat cttagtgaac ttgagcaggt gtaatcagat
       61 ggatctaaaa acatttacag cacagatcga actaatgcat caagaagctt taagacaaag
      121 cgcctcgtac gaagacaagt ggctcaatac gttccatggt ggacgtgaga gcgcacttga
      181 tcaagtactc aaattattga aaggggaacg tcgggatgga taagaaagcg gcaatgcagc
      241 gaattatcga attgacttat tcagaagatt ggcaaaatga caaagaagct gcctcggaag
      301 tgatgagact tggaagagcg atgtgggcag acaagagcaa caagccaaga ccgcgaaaaa
      361 tcgcaatttg gcacggtgac aaacttctag tgacagggac agctgaacag ttagcaagtc
      421 tcacaggctt gcacgagaaa atcgtgagaa aaagagctag gtgtggatac acagacgtta
      481 agaagagaac gtttagatac gtggagggat cgtcatgaca acagaagaag tgattcaaat
      541 gcgtattcga aacattcagc gtgaaattga cgatctagaa cgaccaaagg cagtgatggt
      601 caatgaaacg gctagaaagg caatcgattt gcacatagag aacttaagaa gggaaattcg
      661 gagattggag gaatgagcgt ggataaggaa gcggcttggc gaaaattaat gttgctgatt
      721 caagatgaga actggcaaga agatgaagca gtggttgctg aagttcagcg tctaggaaag
      781 attgctaacg gacgtatacg aaaaaagcca gacaaaagaa aacagcgcaa agggaaaatc
      841 gtcgttgttt tacacgaagg caaaattttg atgcaaggaa cagctagtga gctgtctgca
      901 gaaactggat atacgcgtgg gactattcga acgtacgctt ggcgaaatca caccgatcga
      961 aaagggcacg aatataagta tttggaggaa gaaaaatgaa cgaaaacaaa ttaatcaaat
     1021 tgggtgtagc aggagcagta atcgtaggta ttggagttat cggaggattt aagttcttcg
     1081 aaaaaatcga taatggatat gtgggtgtgc gctattcaat gaacggcggt atcaaagatg
     1141 aagcactgac gcaaggtgtg aaatttgtag ggattgacaa agtgatccaa tatccaattc
     1201 gcttgcaaac tatccaatca aaaaatattt cagtatctac aagcgacggc aaaaagacaa
     1261 cgattgatat caaatatgac tacaaagttg attcaactaa agcagcaaaa atgtacaagg
     1321 aatttgggaa tatcacttcg gaagatatcg aaagtggatg gttaaaatct aagcttcaaa
     1381 aggtcgctcg tgaagtttat gcgaaatata gtctgcttga tgtcctttca ggagattcct
     1441 ctaaagttga agctgaggta ttaacgaact ttgctaaatc agttgaatct aaagggtttg
     1501 aagtcgaaga cgtaacactt ggtgttccag atgtcgataa agaaacacaa aaatcaatcg
     1561 atgcgatcat tcgagctggt caagaaaatg aaaaagcgaa gctagacgca gaaactgcaa
     1621 aaactcaagc tgatagtgaa gcttacaaga aaacaaaagc tgcagaagca gaggcagaat
     1681 ctaatcgcaa agtcgccgaa tcagtaacag acaatttgat tcgttatgaa gaagctcaag
     1741 ctcgcaaaaa gcatggatgg gtaacagtaa atggagcaga tactgtagtt acggatgaag
     1801 caggcaaata atatgggatt ctttatggct aaaattctct tgttcttagg tttagtagga
     1861 gcagcatatc tcgtgtatgc gctcctttcc caaactgatg acaaagaaga tgacaacaac
     1921 gatgaaatga aattttagga ggagaaataa tggacgaact aatcacaaaa gtagagcagt
     1981 gggctaaaga caagggactg gatcaagcag atccaaaagc acagtttttg aaagtagctg
     2041 aggaattcgg ggaaatagct tcggcgatgg caagaagtaa tgatgaacta tttaaagata
     2101 gcgtaggaga cgttatcgtc acgctgatta tcctttccat gcaaaaaggg acaaacgtac
     2161 aagagtgttt agaaatggca tacaacgaaa tcaaaggacg cacagggaaa atggtagatg
     2221 gtgtattcgt gaagtcgagt gatttggagg atgtgaaatg aaatacgaaa taccactaag
     2281 tgaagcgggc attcaagcaa ttatcaatgg tcgggaggtt aacatagaac ttcctgatgg
     2341 tactgaatta gtcatcagac aaagttattt gaaagatatg gcagctccag tattaattga
     2401 tcgttttaac gtgactgatt ctgtggtaga gaaccactta aaagaatttc gatcaagtat
     2461 agacaacact ttcagattag ggagttgatt gacaatgaac accagacacc gcagagtagc
     2521 aaaactaaga aaacaggaac taaatgtact aaagacaaag tttgaaaaag aatatggaat
     2581 ttcagcagaa gaaacatata aagtggcaag tcagtttgtt gctgatgcaa gtgatgctat
     2641 tcgtaagttt gggatttcga tattaaatga tgatcgtaaa tgggaggaaa tgagatgaaa
     2701 ctaaaagacg gattttacgc tagtagtcac ggcatcggcg gtttaatgct agatatgccg
     2761 acaaagaacc ctaaaacacg taagaaacca aaagtcaaag tcggtgacat ggttcgctgt
     2821 gaagcagaag agttcattta tccgtttcgt ggatatgtag aacacgtcta taatcactca
     2881 gcgatcattc gtattgaaaa cacaatggaa tgcgataagt ggttagcgaa aagcaaagag
     2941 aatttagctg tagcgagatt ggtggatatg gaggttataa acaatgaagt ttaaaatctt
     3001 tgaagaggac actcgttata aattagaaaa agaattaaac gattttgcga aaaacaatga
     3061 gattcagcat atatctttag caacttctaa gagaggttat gcaaattact atgcagctgt
     3121 tgtgagctat gtaagtcgag aagtgtaact cggcaaataa aaaaagccgg atcgctccga
     3181 ctgattcaat aaatccaaca catttattat atcacataaa ggagcggttt gacttgatgc
     3241 aattgttacg agaggtagat ttcaaacaga caagatgtaa tgcgagagat gtgctgaaga
     3301 actttcggcg tttggagcgg atggcaggtc gctctttgat agatattaag tcgccgatta
     3361 ttacggatat gccgaaggca ccgaagcacg gcaataaggc agaagacgcg atcattcaaa
     3421 tgatggatat agaagcggag agagacgcga ttctagtggc tttgatggct cttagtctga
     3481 ttagtcgtca gatactctac tacagcttct gtgtgccaga tagcttctca aactacagga
     3541 ttagccgtga agtgggttat tcagaaagaa gcatacaaag gatgaagtcg gaagctctaa
     3601 tagagtttgc agaagcgtat aaacacggaa gaataattgc ttataaatag ggggaccata
     3661 taatgtggaa agattatgta tcgttgaaag agttgaaaaa agatcttgtt ttcaaaagaa
     3721 ttgtagaatg gtcagagagt gaattaattt tagaagatgg aaccaaaatg gaagttgtat
     3781 gtagcgaatc agattgctgc gcatgggccg aaggtgaatt caaaaatgta aagcttgatg
     3841 cagtgattac agatataaag atttttgata aaggtaaccg tctctataat ggtgacggac
     3901 atacctctta tgctgaagtc gttgtttatc ataatcgtaa tgagatcgct aaagcagaat
     3961 gcacagcgaa tgatgggaat ggcggctact attatagtgt ttgtgcttta aaggtcaaag
     4021 acaaactttg tatagtgact gacgcataaa aaaatggcgg ttttttggcg gaatgatggc
     4081 ggtttttagc catttatcag tgatattatg gtagtgtcga aagattagtg ataggtctga
     4141 gacaaaataa taataaaagg aacatcgttt tattattgtt tcacaattaa gcttcgatag
     4201 acagcaacgg aaatattaag aataaggatg tgaattttaa ctccttctaa attgttctta
     4261 ttatctatca tccgttgctg tctattgtta catacagatc gcttaggcgg tctttttatt
     4321 ttggccgaaa acctacattt tcgatggccg attattggag gaggaatagc atgttcaaac
     4381 tatctgaaat cattaagaag tccgacgttg agaaattaga gatgttaaaa aagaagttga
     4441 agaaatagca gtctcttcgt gaggctgttt tattttgctc acaaaaatag accactatcg
     4501 ggtaatagtg gtcaggaatt aaatgaaaaa gatgttaggg ttgttagcta agtataacat
     4561 cataacgctt acaaaacaat gcaagaagga taaattatta tgagaactta ctggtatgtg
     4621 tcattaaaca ataaatatcc gaagccaatg aaaggacagc ataggcgtgt agtcatgtct
     4681 gttca
//
LOCUS       DACEPW010000174         4675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEPW010000174 DACEPW010000000
VERSION     DACEPW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.179"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            385..1518
                     /locus_tag="IW819_002320"
     CDS             385..1518
                     /locus_tag="IW819_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1245408.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1549..1869
                     /locus_tag="IW819_002321"
     CDS             1549..1869
                     /locus_tag="IW819_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245409.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(1898..2362)
                     /locus_tag="IW819_002322"
     CDS             complement(1898..2362)
                     /locus_tag="IW819_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1245410.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2494..3747)
                     /locus_tag="IW819_002323"
     CDS             complement(2494..3747)
                     /locus_tag="IW819_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1245411.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(3776..4093)
                     /locus_tag="IW819_002324"
     CDS             complement(3776..4093)
                     /locus_tag="IW819_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1245412.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(4119..4439)
                     /locus_tag="IW819_002325"
     CDS             complement(4119..4439)
                     /locus_tag="IW819_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1245413.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         1485 a          943 c          836 g         1411 t
ORIGIN      
        1 cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa
       61 atttgactta ataccgagaa actgatccct tctgtcataa cctcttttta tttatgtctg
      121 tttctttttg aaaacaacta ttttttcaac gtttactctc ttgccaatcc aacatcatct
      181 aacggataca actatttcaa tgattaccat tacactaaaa aatcggaaac acacctctcc
      241 gaacacaaat acataagact atagaatgtt tcatttaaga aaaaaagcgt ttcattatca
      301 aaaaggtaag atctccttta aaatgaaatt atctgtagta ctaataatgc agacaatttt
      361 atgaatctga aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg
      421 gaaatcagaa acgtagatga tccaaaaata ttagaaccaa cagatgcaat catccgtatc
      481 acagcaacag ccatttgcgg ctccgatctg catctttacc atcatggtga agcagttttg
      541 gaaaaagggt atgttgtagg acacgaacca atgggaatcg ttgaagaagt gggatctcaa
      601 gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag ttgtggtcat
      661 tgtcatttct gcctgaatca catggaaagc caatgtgatg aatcaaatcc agatcagtta
      721 tatggcgggc ttttcggatt tggcaagttg aacggtaatc attggggtgg tcaagcggaa
      781 tatctaagag ttccttatgc agactctact tcatttgttg taccagataa tgatcttcct
      841 gatgaaaaag ttttatttct atcagatatt ttgcccacag cttattggag cgttgaaaac
      901 gcaggagtaa aaaaaggcga tacagttgtg attctaggtt ctggtccagt cggtctattc
      961 gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga tccagtacag
     1021 caccgtctag ataaagcggc taattacaat ggggttgaaa cttatttctt agaagacacg
     1081 gcacaagctg gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt cattatcgat
     1141 tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag cgaaaaattt agtaagctta
     1201 caaagcggca caatctctcc attacagatg gcatcgcaag ctgtcaaaaa atttggaaca
     1261 gtccaagtaa ctggtgttta tatgactcca gcttcttctt acccgctgca agaatttttt
     1321 atgcgggata tcgctgtcaa gcatggacag gcaccaatta tccatctaat gccaaaaatt
     1381 tacgcaatga ttgcagaagg actattcgat cctacacaaa tcattactca ttcgctgcca
     1441 ctatcagaag cagcacaagc atatgagatc tttgacaaaa aagaagataa aaatataaaa
     1501 gttgttttga aaccttgaat tcagtaaaaa atacaggtga ttttctttat gcccaaaatc
     1561 aaacgaaata cccatgagat cgaaatagga aaagaagaag atatcgaaat acgaggacgt
     1621 cgcttccgct tctatttcca aaatcgttac acattgatct cactggctgt cgatttatta
     1681 accgggattt tctatatttt tggaagcctt gcttccttaa ctaaaatccc cgatcgatat
     1741 ggcacctatt tttatttggc tggtgctatt ttcttgacga ttcgtcctat tctcagaatc
     1801 gtaaggaaca tttttattta cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa
     1861 gaggcatgag aatttttcaa atctcatgcc tcttttttca ccctatcgta tcactttgat
     1921 acagacgagc ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc cactcgtatc
     1981 gttcataaaa cgtggtatga tctgtaatta agtaaagttt ttcgtaccca agttgatgca
     2041 tttcttttct gatttccgct aatatccgct tggctatccc ctgcttacgc ttttccggtt
     2101 caacaaacag ggcacaaaga tttggtgtca gatcagggcg gttatggaag tcgttttcaa
     2161 tgacgcccgc tcctccgacg atggtttctt tttcatctaa gaccacatac cattgaggaa
     2221 tactttcctt attagcgatt ccagtacgaa tactttcctc atacacaaca gcaggaactc
     2281 cccattttga tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt
     2341 tgatccagtt atatgtaacc aatgtttaaa cctccgacta aaataaaacg tttacaaaaa
     2401 ttatacaagt ctttatgaaa attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg
     2461 aaaacaatca cttgttctcc gttttttgat taatcaagca tctaaagctt cttttcttct
     2521 ttcttggatc gtagctacct tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt
     2581 gacagcggct agtacaccac ctgcaatact ttttgttgcc aatacaccgc caataattgg
     2641 tggagttacc catggaggca taacggtagc tgctggcact aaacctgtag aagtcgcaaa
     2701 gtaagcaaca ctaactaaga ccattggtgt caaaataaat ggaatgaaca tgatcgggtt
     2761 caacacgata ggtaacccaa acatcgttgg ttcattaata ttgaagacac ctggcgcaat
     2821 cgacaagttt gttacaacca tatatggttt gtttttacgt cctactaaat aaatagctaa
     2881 cagtaaacct aaagttgcac ctgttcctcc taagttgaca aagctgtcaa agaaaggttt
     2941 gttcacgata aacggtgcgc tatgtccagc tgcgattgca ttaacgtttg cttcaatggc
     3001 tggcgcattg attgtttgca tcaaaggatc gaccatattc gctccgtgta aaccaaagaa
     3061 ccataagaat ggtgtgataa atgcaagcag taaggcagat ggataagagt ttgcaagtcc
     3121 cataaatggt tcttggacca ctgtatagaa agaacctacg atatcatcaa caccgaatgc
     3181 agcaaaaatt gcagcaacta aagcaaacgc agagatagtg atcattgctg gtagtaatga
     3241 agcaaatgct tttgcaactg ccggtggcac accatcaggc attttgatga tcaatttttc
     3301 atttccgctt aaacgtacaa agatttctgt tgaaataatc ccaacaataa tagcaataaa
     3361 caaaccgttt gagctcatac ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat
     3421 cacacctgcg gaaacaccgt cttttcggta gccgttagct aaatgataag caagcaagaa
     3481 agcaatcaag acagaaagaa tggcaaatgt accgttccaa gcagcagccc cgaatccttt
     3541 ccatgattca ccaccaaaaa ttgagttcat caattcttgg aacccgggaa tcggtaagtt
     3601 attgatcatt actgctaatg ctcctaagat cattaacggc atactcacca taaatgagtc
     3661 ccgaatcgca actaaatgcc gttgtgcacc aatttttgat gctacaggta taaaatgttt
     3721 ctccataaat gcaacaaatt tatccataaa tcttccctct tttctttact gtagattacg
     3781 cgttcaatcg acgatacaga tcaatgatct cttttgctaa atctgtaaaa gctatagatg
     3841 tcatcagatg atcttgactg tggaccgtca aaagcgtgac ttcaacgtga tttccttgtg
     3901 cttcttgtgt cagcatctct gtttgagagt gatgtgcttg gactaaagat tcctctgcat
     3961 ctttgatttt ttcatctgct aaagtgaaat cacctttttt cgcagcagca atcgcttcca
     4021 ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc catgatggct tctagatttt
     4081 tttgatcttc catttattgt atcctccgat aatttttatt atttgtttat tagtttttct
     4141 gcttggtcga gtactttttc accattcatc atgccatagt cagccatatt gattacatct
     4201 aaaggaatgc ctttaggtgc tagcttttca gcaaattgat tttgcataaa gcgtacttgc
     4261 ggtcctaaaa gtaaaacgtc tacattttta gattctagtt gattatccgc atctgaagca
     4321 gatacagcaa agatgtctgc ttccattcct tttgcttctg ctgccttttg catttttgtg
     4381 actaggagac ttgtactcat tccagctgaa catacaagca tgattgtttt cttttccatt
     4441 ttaaacacgt tcctttctat acttacactt gtatataagc aagtagcgtg ccaactttaa
     4501 aagcgcttta aaaagttgat atcatcacat ttaaggtcta taccaagttg catacacact
     4561 aaaaaagaaa gcgcacacta aacacacaaa ttaattttta ttaccttttt tcttttaacg
     4621 tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt tttcaaataa acggt
//
LOCUS       DACEPW010000175         4675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEPW010000175 DACEPW010000000
VERSION     DACEPW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.180"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            79..1125
                     /locus_tag="IW819_002326"
     CDS             79..1125
                     /locus_tag="IW819_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1245414.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IW819_002327"
     CDS             1474..2106
                     /locus_tag="IW819_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1245415.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IW819_002328"
     CDS             2694..4133
                     /locus_tag="IW819_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1245416.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IW819_002329"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IW819_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1524 a          740 c          995 g         1416 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taaga
//
LOCUS       DACEPW010000176         4498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEPW010000176 DACEPW010000000
VERSION     DACEPW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.181"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            264..1457
                     /locus_tag="IW819_002330"
     CDS             264..1457
                     /locus_tag="IW819_002330"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1245417.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1587..3059
                     /locus_tag="IW819_002331"
     CDS             1587..3059
                     /locus_tag="IW819_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1245418.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3142..3930
                     /locus_tag="IW819_002332"
     CDS             3142..3930
                     /locus_tag="IW819_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1245419.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3997..>4498)
                     /locus_tag="IW819_002333"
     CDS             complement(3997..>4498)
                     /locus_tag="IW819_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1245420.1"
                     /translation="MAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEI
                     NTVPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKI
                     MDGNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNK
                     RRENEQ"
BASE COUNT         1347 a          738 c          994 g         1419 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1501 ctttttttat ggcagttaac ctcttgaaaa agatagtaat tatttatcta tttttagatc
     1561 ctgtcataat gaaagggagg aataagatga aaaaagaaac aaatgttact ttggtcacag
     1621 tgagtatttt cattgcaacg ttcatgacgg ctattgaagg tacaatcgta acaaccgcta
     1681 tgccaacgat tgtcggttca ttacatggaa tggaaatcat gaactgggta ttttcgattt
     1741 atttgctgac aaacgctatg cttactccga tttacggaaa acttgcggat aaagtgggac
     1801 ggaaaccaat ctttattttt gggacattat tgtttattat cggttcgtta ttttgcggtt
     1861 tatctcaaag catgattggg ttgattgctg ctcgtgcgat tcaagggatg ggtgctgggg
     1921 caatgatgcc agtagctttg acgatcatcg gcgatcttta ttctattgaa aagcgagcaa
     1981 aagttttagg tttgaatagt tcagcctggg ggattgccag tgtttttgga ccgcttgcgg
     2041 gaggattcat cgttgaaacg atcagctggc actggatctt ttttatcaat gtgccgatcg
     2101 gaattctatt aattattttg atttggctat atttaaatga accaaaaatc atacatgagt
     2161 ctaaaccaat ggatattttt ggtagtttta gtttgatggc catgcttgtc tcattgctgt
     2221 tagggttcca attattagga gacgaaggaa tgagtccgct tgtttctgct ttgctgctag
     2281 gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa aagagctaaa gatcctgtca
     2341 tcatgatgca tttattcaaa aatccactat ttgttgtggt caacttggct gcagcattgg
     2401 ctagtggttt tttgatggga atcgatgttt atatccctat gtggatgcaa ggtgtattag
     2461 ggctgaatgc agcaattggt gggctggtat tagcaccatt atcactagtc tggatggtcg
     2521 gttctttcct tgcaagcaag tggatgactc aaatgaaaac tcattgggtc ttgagaattg
     2581 gactactaat cgcagctatt gggggcgctt ggttgtatct tatgcctttc agaagtaact
     2641 atttatggtt ttttgctata tcaacggtca tcggtatagg cttaggtgtg accatgacta
     2701 cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca gcttggtgta gcaacttctt
     2761 ttaatacgtt agttcgaaca attggtcaaa cagtcatggt ttctattttt ggtttattga
     2821 taaatggggt aactaaacga gaacttgcag cagcaaattt aacaaatgat gcagatatca
     2881 tgaataaact ggtgaatcca caaacggcta aatatattga tgcggatatt ttgttaccat
     2941 taagaaaaat tctttatgat gggttacatt ccgtttatct agttggcttg ttactcgtga
     3001 tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag tggtaaaata aaacagtagt
     3061 ttcggttaag atatgtacgt agccgaatca ttcaaattta aagttattca aaaataactt
     3121 cttactaagg aaggcgaaaa tatggcattt attcaagcaa atatttattc gaatgtgtta
     3181 gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa caaaaaaggt gattggcacc
     3241 agttcagagc aagagacaac ggatatacct gttttatatt tacttcatgg aatgggtggc
     3301 aatcatagtg tatgggaaag acgtacgtcg attgaacgtt atgtagcaaa ctacggatta
     3361 gctgtcatca tgccatctac tgatcttggc tggtacacag atacagttta tgacatgaag
     3421 tattggacat tcattgcaga agagctacct gagatttgtc atgaattatt cccgcaatta
     3481 acaagaaaaa gagaaaaaac atatgccgct ggattatcga tgggcggata tggcgcgtta
     3541 aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa tctctctttc aggcgctgtt
     3601 tctgtaggaa atcgaatgga tgatcttttg atggttcgta aagcatcttt ttgggaaggg
     3661 atctttggac cattggatca aatcgaaggt tcaaaaaatg atccgcaata cttattgaaa
     3721 gaactagtaa aaagcggaaa accagttcca agattttata tggcttgtgg ggaatctgat
     3781 tttctttatc aggcaaatca ggaaatggta tctgctatgc aggaacaagg cttggcggtc
     3841 acctttgaac aaggacctgg cgatcataac tggatcttct gggaccaatg gattcaacgt
     3901 gcattggcgt ggctaatgga agaaaaataa aaaaagagtc ttaaaaagat agctcctata
     3961 gagatcacaa acatagtgat ctctatagga gcttttttac tgctcgtttt ctcttctttt
     4021 attttgttgg cataagcctt caatataggt ttgtgcactc gttagaaaga gtgctttttc
     4081 attcaattct gcatgggact gcatactttc tttaaatccc tctaattgat tttcaactac
     4141 ataattacgc ataaatagaa ctgtttcttt caaaaattca tttccgtcca taattttttg
     4201 tcgaagctgc tgttcatgct gctgatccat taactgtcca atcaataaat tgtggagaga
     4261 ggcaatggcc tgatgacttt gggtgactga aaatccttca cgaacaagta attggatcat
     4321 ttgctccaaa tgttctaatc gactcggaaa agcgggcaca gtattgattt ctaattctgc
     4381 cccacaaggg aaacgtgtat aaaggtcata gtaattttcc atatactgca gcaactgttc
     4441 attccaaggc aaagttggat ctggaagcgc taattcttct tccattgttt cagccatt
//
LOCUS       DACEPW010000177         4487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEPW010000177 DACEPW010000000
VERSION     DACEPW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.182"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..132
                     /locus_tag="IW819_002334"
     CDS             <1..132
                     /locus_tag="IW819_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1245421.1"
                     /translation="DFEGPTKKYYEKYAGMTAFRCVAKFPKYPKYAKVQPKTDVVVK"
     gene            165..515
                     /locus_tag="IW819_002335"
     CDS             165..515
                     /locus_tag="IW819_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1245422.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            549..1487
                     /locus_tag="IW819_002336"
     CDS             549..1487
                     /locus_tag="IW819_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1245423.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            1565..2458
                     /locus_tag="IW819_002337"
     CDS             1565..2458
                     /locus_tag="IW819_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1245424.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            2496..3059
                     /gene="amaP"
                     /locus_tag="IW819_002338"
     CDS             2496..3059
                     /gene="amaP"
                     /locus_tag="IW819_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1245425.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            3073..3261
                     /locus_tag="IW819_002339"
     CDS             3073..3261
                     /locus_tag="IW819_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1245426.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            3274..4176
                     /locus_tag="IW819_002340"
     CDS             3274..4176
                     /locus_tag="IW819_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1245427.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            4208..4450
                     /locus_tag="IW819_002341"
     CDS             4208..4450
                     /locus_tag="IW819_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1245428.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         1623 a          781 c          932 g         1151 t
ORIGIN      
        1 gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac ggctttccgt
       61 tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa aacagatgta
      121 gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa attacaatgt
      181 ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa atcgcactcg
      241 cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca aaaggcagct
      301 tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa atcgagcatc
      361 cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa cgattgaaca
      421 tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca gtactcgatt
      481 ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt ccaaaaagga
      541 ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa acagatgaaa
      601 aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag caagcaatcg
      661 aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa gaagtttctc
      721 ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta gtgattaatc
      781 tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt gtactttccg
      841 ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg ctgctgacaa
      901 agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta ttagatattt
      961 acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct ttggataaag
     1021 cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt gaaagagaca
     1081 tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa ttatggaatt
     1141 ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc catttgagac
     1201 agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt attggtgatc
     1261 tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta gattcagctg
     1321 cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat acaggaggat
     1381 taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct ttgatagcag
     1441 cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga atctatttaa
     1501 aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta ggaggaatac
     1561 agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg agaaatttcc
     1621 taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc ctgattgtgg
     1681 agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa tcactggtgg
     1741 agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg cagatatcgc
     1801 tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat acgtgaaagt
     1861 tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag cacctaaaga
     1921 aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac taaacgcagg
     1981 acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca aagacacatt
     2041 tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc atatcccagc
     2101 aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg aacatttatt
     2161 agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag caaaacagct
     2221 tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga caccattaca
     2281 actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa gtttactaga
     2341 acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat cgaataaagc
     2401 aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca acttataaga
     2461 agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta aaaacgatcg
     2521 gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta ttaagtgcaa
     2581 cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact aattcatacg
     2641 tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt atcacgttac
     2701 ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat ggtgatgggg
     2761 agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg catgaagatg
     2821 aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc gccgtcaatg
     2881 taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca ttgatgaaag
     2941 aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa gtatcggtga
     3001 aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga gtagaatagg
     3061 gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat ctttggggta
     3121 cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac cttattgatc
     3181 ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac gaaaatctta
     3241 gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa aaataataac
     3301 aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac accaaaccaa
     3361 tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca tccagatcca
     3421 gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga aaagcatgca
     3481 cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc aatcgatcct
     3541 tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca agaaaaactt
     3601 agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa taaccaagga
     3661 aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga agataaagtt
     3721 gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc tgcaaacggt
     3781 ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac ggctgggatc
     3841 gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt ggaatatggc
     3901 aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga agtaaaaaat
     3961 atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa aacaaaagaa
     4021 gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc gaaaaaaacc
     4081 ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag cggcgcacaa
     4141 aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac attaggagga
     4201 acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag caattgcagg
     4261 agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag caggtttagt
     4321 tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg caggaatggc
     4381 aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat tctttgctcg
     4441 aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttct
//
LOCUS       DACEPW010000178         4479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEPW010000178 DACEPW010000000
VERSION     DACEPW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.183"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(109..2235)
                     /gene="pnp"
                     /locus_tag="IW819_002342"
     CDS             complement(109..2235)
                     /gene="pnp"
                     /locus_tag="IW819_002342"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1245429.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2423..2692)
                     /gene="rpsO"
                     /locus_tag="IW819_002343"
     CDS             complement(2423..2692)
                     /gene="rpsO"
                     /locus_tag="IW819_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1245430.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            2943..3506
                     /locus_tag="IW819_002344"
     CDS             2943..3506
                     /locus_tag="IW819_002344"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1245431.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3759..4280)
                     /locus_tag="IW819_002345"
     CDS             complement(3759..4280)
                     /locus_tag="IW819_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1245432.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4283..>4479)
                     /locus_tag="IW819_002346"
     CDS             complement(4283..>4479)
                     /locus_tag="IW819_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose acetyltransferase"
                     /protein_id="HAQ1245433.1"
                     /translation="DTVTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETIT
                     DTYVDLMKHSREYQNKMKEE"
BASE COUNT         1163 a          957 c          839 g         1520 t
ORIGIN      
        1 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
       61 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
      121 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
      181 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
      241 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
      301 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
      361 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
      421 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
      481 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
      541 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
      601 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
      661 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
      721 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
      781 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
      841 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
      901 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
      961 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
     1021 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
     1081 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
     1141 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
     1201 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
     1261 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
     1321 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
     1381 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
     1441 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
     1501 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
     1561 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
     1621 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
     1681 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
     1741 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
     1801 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
     1861 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
     1921 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
     1981 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
     2041 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
     2101 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
     2161 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
     2221 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
     2281 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
     2341 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
     2401 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
     2461 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
     2521 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
     2581 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
     2641 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
     2701 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
     2761 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
     2821 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
     2881 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
     2941 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
     3001 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
     3061 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
     3121 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
     3181 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
     3241 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
     3301 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
     3361 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
     3421 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
     3481 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
     3541 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
     3601 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
     3661 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
     3721 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
     3781 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
     3841 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
     3901 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
     3961 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
     4021 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
     4081 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
     4141 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
     4201 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
     4261 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
     4321 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
     4381 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
     4441 cttctttttt cacttgtcct attttagtta ccgtatcca
//
LOCUS       DACEPW010000179         4475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEPW010000179 DACEPW010000000
VERSION     DACEPW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.184"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            338..1069
                     /locus_tag="IW819_002347"
     CDS             338..1069
                     /locus_tag="IW819_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1245434.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1214..3241
                     /locus_tag="IW819_002348"
     CDS             1214..3241
                     /locus_tag="IW819_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ1245435.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3349..4446
                     /locus_tag="IW819_002349"
     CDS             3349..4446
                     /locus_tag="IW819_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1245436.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1579 a          720 c          920 g         1256 t
ORIGIN      
        1 aacgataatc acaaaaaaaa atataatata cattttttat ttaattataa ttaatattga
       61 ttgaagtcta ggcagtcaag tcacattttg atagatagga gggggactta cgaattattt
      121 taaaggctat gacagctgta tttctagatt gtgtttgcat caagttgcaa taaaaactag
      181 aaatcaaatc gggctgataa tcatttataa ctaatcgata cataggacca aatataggtc
      241 tatgttcttt tatttcataa gtatcgtaaa acagaatttt gctatggatt aatccaatcg
      301 actgatatgt ttagtaaata aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt
      361 aacagcagca atgatgttat tcagcatggt agcaagtacg acttttgttt ctgcaactga
      421 agtataccca aaggaatata acacagaagg aacgattaca tttgaagcag gagatgaggg
      481 tgtaactcct cctgttgatc cagagaatcc tgatcccaat aaacctgtcg atccatcaga
      541 tcctccaagc cctggaacag gaggggcatt atcgattgat tatggatcaa aattcaaatt
      601 tgggactcaa aaaatttcta ctgcggataa aacgtattat gcagctgctg atgtgatgaa
      661 cgatgggtct agaaaaccga catatgtcca agtgacagat aggagaagta ccttatctgg
      721 gtggaaatta agtgtctctc aaccagaaca gtttaaaact gccagtgggg atgaactagt
      781 tggagcacag ctgaaattta cgaaagggca ggctgtttct ttggttgatc caacatatac
      841 gccgcaaacg gtgaattctg aattaacgtt gacaccggga ggaaacaata cgttagcaat
      901 taatgctaaa tcaggaacag gtgtggggac ttgggtctat cgatttggtg caaatgaaaa
      961 tgaaaatcaa gatgcggtac agctttcggt acccggtaaa tctgtcaagt tagcgcaaca
     1021 atattccact aaattagttt ggacattaga agatacacct aacaactagt ttactgaaaa
     1081 gaactgttaa aaataaatac gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt
     1141 caaaggctag gctaaatttg tttggatacg aatagttcat ctaggaattt aacgatcagg
     1201 aggaaaagat atattgaaaa agaggaggat cgtgctcatt ttaagcagtc tcttagtgac
     1261 taatatcttt ccgatcatag ggtactcttt ggaattgaat cagttggagc atggaaatag
     1321 aagagacgaa gaaagcaaac tcacttcgac attcgacagt ttaatagaat ccaaccagac
     1381 acaagaaagc agtgaggatt taagtatttc gagtagtgga gagaccactg acaacgtaga
     1441 agctgagggt tcggacaaac aaggactaga agatgcacca atttctgcgg ttgagctgcc
     1501 tgacgatccc aatattattt ctatcgataa aatttttcaa acacctatcg gtactagcac
     1561 gtccatattg gaaaatggga agttactgca gttgaaccca gcagaaaggt ctcaaagagg
     1621 agctatttgg tcaaataaga agattagttt actctcggat tttacattta aatcttattt
     1681 atacttagga aatcaacgag atagagcggg agatggcatg acctttacat tgactggcga
     1741 tcctagaatg gctagtaatc ccagtcaagt tattggatcg ccaggtatgg gaattggctc
     1801 atattctact cgttctggcc aaccttacat tgcgaatgcg ctctcgattg agtttgatac
     1861 gtataaaaat agtggttctt ctgatcgaat ggatcgagag ataactgctg ataatagagg
     1921 gcatggacat gtcgcttttg tcacgcctaa accaaataat aataattatt ctggagagca
     1981 tactggcgtg acggtagctc ctacatttct ttctaatggc acatggcgct tgttaataat
     2041 taaatgggac gcggagcaaa gaagtcttac ttatgatttg gaaggtgtcg gtcgctcttc
     2101 tcggattata cctgatctca ttaggcagtt tggttctaca gaagtaaatt gggggtttac
     2161 ttcttcaaca ggtacttttt ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa
     2221 tgtcacatca acagctgatg taaaagtcaa cgatagtgat tatggggcca tcgctgaagc
     2281 tggaggaaac gataagataa ctatcagaaa tacattgaag gtcgaggggg attttcttga
     2341 atataagaaa atgcagacta gtatcgacct cccacaagaa ttaactattc ccgagcatat
     2401 cttgatcaat ggaaatcaaa ttgatgccaa agatatcttg ataaccggaa atcgagtcac
     2461 gctaaatctg acagattatt ttattgctgg agatacgtta ataatcgaat tggagacaac
     2521 tttgcaggaa gtaattcctg aaaaagtact cactatgcat tttgaatacc ttgaaaacgg
     2581 gaatttgatt gagcaatcga atgaaataaa cctaaagatc gcaaagctga aggagataga
     2641 tatccatgtt tattataaag ataaagacag tcagattgat attgctgaaa tgaaaacaat
     2701 cactgggaaa ataggagaga agtataaaga aactcctatt gatattgaag ggtatgtttt
     2761 tgactcggat tcagcgaata cagaaggcat cttctccgaa aatacagagg atatctattt
     2821 ttattacaga aaaggcaaac tttatctctc aactgctcct caatatgtca attttggaaa
     2881 acataaaata tccagtagac cattaagtga attcggacag ttttcaggtg ggttgaaaat
     2941 tatagatgaa cgggcttttg gaagttggaa attacaattg aaacaagaga aagttcttac
     3001 aaatggagat tttgagcttc cagaagctct ttcctttgtt acctctgaaa atacaacggt
     3061 tatagggaca tccgctgtta cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc
     3121 ctctctttta gattctacag ctcatcgtgg aatcagaata gatattcctg tagaaaacca
     3181 aagaatcgga acctttgagg ggaaactatc ttggatactt gcagatgtcc caggaaatta
     3241 acaggaagag aagaatataa caaaattatt gagtttcaag aagggcacag gtaccatagg
     3301 ctcttgtgtc ccacagagac aagattttgt tatagtcttc tcttacatat gattaaagga
     3361 gcgattttct tgagaccaaa acaacaatta tttataacac tcctagttat tttaggggca
     3421 ctttttaaca atatatcagt gtcagctagt gcatttaatt tttcagtgac gccgataact
     3481 tcagagaatc aaatcgacaa gagaaaaact tacttcgatc ttcaacttga tccggatcag
     3541 gaagtagaag taaaagcgga attgagaaat gatacagaaa aagaagtgaa aatcgatatt
     3601 tctgtgaata gcgcaaccac caattcaaat gtgatggttg aatacggaaa aaacgagatt
     3661 gaaaaagaca tgagcctgat ctttgatctg gtagattatg tgagctatcc acagatagtg
     3721 acgctaaaac ctaaaagtgt ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga
     3781 tttgatggtg tattggctgg tgggatcaca ttccaagaaa tccagacgga aaaggaccaa
     3841 acagaaacta aggatcaaag cttatcgatt gagaatgaat acacctatat tattgctttg
     3901 ttgatgcaac agacactgaa cgaagttgct ccgaacctta ctcttcatga agttaaacca
     3961 gatcaaatca atgcaagaaa tgtcatctta gcaaacgttc aaaatgatca aaaaacctat
     4021 atcaatcaag tagttattga aacaaaaatt acaaaaaaag gacattctga agttttatat
     4081 caagaggaaa aagaaggatt gcagattgcg ccgaatacaa atttttcttt tccaactgct
     4141 ttaaatggtc aaccgctaac cccaggtgaa tatcacctta caatgactgt tttaggaaat
     4201 gaaaatgaaa atggaaaatt ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat
     4261 caatgggtat ttgaaaaaga ttttacaata gacggaaaag tagccaagga attgaacacg
     4321 acggatgtta cgattaaaaa agacaataat tggatttata tcttagtagc ttcacttttc
     4381 ttattgttta ttttattatg catacttttg tttgttttga gaaaaaaaga agagaaagaa
     4441 aagtaaggtt aagataacaa atagtaaatt tttat
//
LOCUS       DACEPW010000180         4465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEPW010000180 DACEPW010000000
VERSION     DACEPW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.185"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            574..1008
                     /locus_tag="IW819_002350"
     CDS             574..1008
                     /locus_tag="IW819_002350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245437.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IW819_002351"
     CDS             2298..3221
                     /locus_tag="IW819_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1245438.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IW819_002352"
     CDS             3237..3776
                     /locus_tag="IW819_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1245439.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          674 c          757 g         1367 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tatgaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacacccca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttga cttagaccaa tcgaaaatca caaagtgagc ccctgtatcc cttggtagag
      421 aggctacttg gcatagtaca cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctaa
     4261 acggtcggcg aagaaaacat accaagcacc acaagcaaaa aaagttagag taacttccaa
     4321 aaaagaaaaa ttttttagaa caacttttga aaatttaatt ttgatttata tccgttcaat
     4381 ttaaacattt gataaactga aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa
     4441 aaaaagtgga ggtataacct atgtt
//
LOCUS       DACEPW010000181         4389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEPW010000181 DACEPW010000000
VERSION     DACEPW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.186"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            205..435
                     /locus_tag="IW819_002353"
     CDS             205..435
                     /locus_tag="IW819_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1245440.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            532..1833
                     /gene="murA"
                     /locus_tag="IW819_002354"
     CDS             532..1833
                     /gene="murA"
                     /locus_tag="IW819_002354"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1245441.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1846..2022
                     /locus_tag="IW819_002355"
     CDS             1846..2022
                     /locus_tag="IW819_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1245442.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2157..2453
                     /gene="yidD"
                     /locus_tag="IW819_002356"
     CDS             2157..2453
                     /gene="yidD"
                     /locus_tag="IW819_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1245443.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2517..2900
                     /locus_tag="IW819_002357"
     CDS             2517..2900
                     /locus_tag="IW819_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1245444.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            3069..3953
                     /locus_tag="IW819_002358"
     CDS             3069..3953
                     /locus_tag="IW819_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1245445.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4070..4303
                     /locus_tag="IW819_002359"
     CDS             4070..4303
                     /locus_tag="IW819_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1245446.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1492 a          669 c          959 g         1269 t
ORIGIN      
        1 tgattgaaca ccgtttattt ggaataaggg actgcgacaa gacttttgtc tcagtccctt
       61 tttttgcata tttttagcat ttatttccta aaaaatgtaa ggggattcct atatttttaa
      121 gtatacttgt ttttcctttt atacatttta tggtattatc attttattag ttattataaa
      181 acaatcgtta gaaaggacgt cattatgcaa gtatacggaa ttgatgcggt gcttcgaatc
      241 gtgagtcatt tggcgtttat ctacttagcc ttctggtctt tacgttcgtt acgcattgaa
      301 acttttttca gaccaatgca taatacgcaa attagaatgg caatcgtact tttttcaatt
      361 tttttagggt acactgcaag taattttttc ttagaagtta tcgctttatg ccgaaatctt
      421 ttcgttagcc tgtaaatttt aaaaaaaggt actattttaa aagcaaggat gttataatag
      481 aaaagatttt gagtaagtgt atgctcattg gaattttttg gagggaacaa catggaagag
      541 atcatcgtaa gaggtggcaa tcaattaaat ggaacagtaa gaattgaagg agctaaaaat
      601 gctgttttgc ctattttagc tgctagtttg ttagcagaag aaggtattac gacattagac
      661 aacgttccga ttctttctga tgtcttcact atgaatcaag tgattcgtca cttaaacgta
      721 gatgtagatt ttgatgaaca aaagaatcaa gtaacaatcg acgcttcaag acaattagaa
      781 atcgaagcgc cttatgagta tgttagtcaa atgcgtgctt caatcgttgt gatgggtccg
      841 ctacttgcca gaaatggaca tgcaaaagta gctatgcctg gtggatgtgc gattggtaaa
      901 cgaccaatcg atttgcattt aaaaggattt caagctttag gagcaaaaat tattcaaaaa
      961 aacggctata tcgaagcaat tgcggatgaa ttgatcggta acacgattta tttagatttc
     1021 cctagtgtgg gtgctactca aaatatcatg atggctgccg taaaagcaaa aggaacaacc
     1081 atcattgaaa acgtagctcg cgaaccagaa atcgttgatt tagcaaacat tttgaataaa
     1141 atgggcgctc aagtctatgg agctggtact gaaacaatgc gtattgaagg cgtggatcat
     1201 ttacatgcag tcaaccactc aatcgtacaa gatcgtatcg aagcaggtac ctttatggtt
     1261 gctgctgcaa tgacacaagg caatgtgctg attgcagatg ctatctctga acataactgc
     1321 ccattgattt ctaaattgat cgaaatgggt gcagaaatca ttgaagaaga aggcggagtc
     1381 cgtgtcatcg gaccaaaaca tatcttacct acagatgtaa aaacgatgcc tcatcctggt
     1441 ttccctacag atatgcaagc tcaaatgaca gctatccaat tagttgcaga aggtacaagt
     1501 gtggtgactg aaacagtgtt tgaaaatcgt ttccaacatt tagaagagat gcgccgtatg
     1561 aacgctcatg tcaaaatcga cggaaatgta gcaatcatgg atggaaacca tgaattgcaa
     1621 ggtgcagaag tatatgcaac agacttgcgg gcagcagcag cattagtttt agctggcttg
     1681 aaagcaaatg gaatcacacg tgtgcgtaac ttgaattatc tagaccgtgg atattataat
     1741 ttccatatca aactacagca attaggtgca gacgtcgaac gtgtagacat ggatcaaact
     1801 tctgctgaaa aaacagcaca aacgattgct taaggaagtg gctgaatgag taaccaacga
     1861 tatatcttat taactttgat aaagatttta gtggtgatct tattgcttat ccttcttttt
     1921 gtggccggaa cgatgatcgg atatggtgtc atcggtggcg gcaatccatt taaagtattc
     1981 cagccaagtt tgtggattca tattcgagac ttttttcatt gataaataag aatcacagtg
     2041 gttctagtcg atatgaagtg agccatgaag cagaacatga taaaagaaaa cagaagggaa
     2101 cgttgacgtt agtcgcgatc ccttttttta gtaaaagaaa agaaggaatc taattgatga
     2161 agaagatatt gatttattta gttagaggat atcagcgctt tatttcgcct gcatttccac
     2221 ctagctgccg ttattaccca acctgttcca attatatgat aaaagcaatt caaacccatg
     2281 gtgcgctaaa agggacggta atggggactg cacggatctt acgctgtcat ccttttgtca
     2341 aaggcggtat cgattatgta ccattgacat tccgattaac gcgaaataag aaagataaaa
     2401 aagaaccaga ttataaaaag gttgtgaaaa atacgggtag cactgagcga taaagaaaaa
     2461 gaggaaattt attgtcaaag taacaactcc gcaactactt atatggggga caaaaaatga
     2521 tcgaagaact aaacaagaaa gatttaccat ggtccttgtt attagatgca gatcctgata
     2581 aagaaaaagt tcaagtatat gtttccagag ggagtggctg gatatggaaa gaaaaagaaa
     2641 aaacaatagg tgtactgatc tatgtggcaa gggaaaaaga atacgaaatc gtcaatgtag
     2701 cagtaacccc aacgtatcaa ggaaagggga ttggcggaaa attacttgag acagcttttc
     2761 agaagctttc acagctagct tcaaaccaaa ctcggatcat tatacgtact ggcactacta
     2821 gtagcgctgc gttacacctc tatcaaaaaa tgggctatgt ggagattggt cgagtcaaag
     2881 actattttat ccatcattaa tctgagccca tttttgaaaa tggagatcaa ttaagggatc
     2941 aagtgatttt agcaattcac ttacctaaaa atagctttaa ctaataaact ttcaattcga
     3001 gaaaatgctt tcaaatgcta aatagataca ttacaatagc tttagcaaac aaaaaggaga
     3061 atatttttat gggaatatta gtttttgatt ttggtggatc ggctgtcaaa tatggttgtt
     3121 gggatggcaa agagattaaa ggtaagggac aatttgcaac gcctgaatct tgggaagaaa
     3181 tgaaaacgca attatttcaa gtttataaaa aaatactcgt ttcttttgaa gtcgaaggaa
     3241 ttggaatcag ctctcctgga gtagtgaata atgagaaaca ggtgattgag ggaatcagtg
     3301 cgattcctta tattcatgga tttaatattt tccgagattt ggaaactctg tttcaactcc
     3361 cagtaacgat tgaaaatgac gccaactgtg ccggaatggc tgaattttat gaaggtgctg
     3421 cccgtgatta tcaagatgtt gcttttgttg tggcaggaac tgggattggc ggggcgttgt
     3481 tcacgaaagg acaaataaat aaaggggcac atctttacgg tggagaattt gggttgatgt
     3541 tcttagatgg cgataagaca ttcagtaaac ttggtacagc ggtacagatg gcttggcgtt
     3601 attgtgaacg caaaggtctc gataaaaatg cttataccgg aaaagatgtc tttgaattag
     3661 cagaaactgg agatgcaata gcaaaagaag aagttgagag tttttatacg tatcttacaa
     3721 agggattatt cagtattcaa ttttcgtttg atccagaagt gatcgtatta ggcggcgggg
     3781 tttctgcaaa agaagggctc attgatgaga tcaaaagccg aatgaagaag ctgacagaac
     3841 aattcgatct gcatgatttc gaaccgcaga ttttattatg tgagtacaga aacgatgcta
     3901 atcttgtagg tgcagcagca aactatctcg ctgtacgtca aggagagaat taagagcttt
     3961 agagaaggtg cagcttgcat aaaagaagag aatgaaatga ataggagatt tttacctaag
     4021 gaatgtttta tttgctgcag tatgataaaa tatctgagag gtgaaaatga tgtcaaaaaa
     4081 aaataaagat attgaagtgc gtatagaaga agtgaaaaaa gatatcaaag gtaagaatta
     4141 tgaagtcact cagttattta tagggaaaaa aatgattggt gagatcttag cctatgggcc
     4201 aaaagaatat gaaattttct ttggtgaaga agattttgga aaagaaaaaa gtcttgaaaa
     4261 tgcgatagaa acagtgattc gacactggaa tctgcacgaa taaaaaaata tcaaaaaaac
     4321 tttcaataaa gggttgcaaa aatctggaaa attaggtatt ataactaagt caggttgata
     4381 caaaccttt
//
LOCUS       DACEPW010000182         4374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEPW010000182 DACEPW010000000
VERSION     DACEPW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.187"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..1051)
                     /locus_tag="IW819_002360"
     CDS             complement(83..1051)
                     /locus_tag="IW819_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1245447.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IW819_002361"
     CDS             complement(1498..2904)
                     /locus_tag="IW819_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1245448.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IW819_002362"
     CDS             complement(3126..3488)
                     /locus_tag="IW819_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245449.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3800)
                     /locus_tag="IW819_002363"
     CDS             complement(3492..3800)
                     /locus_tag="IW819_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245450.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IW819_002364"
     CDS             complement(3809..4228)
                     /locus_tag="IW819_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245451.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IW819_002365"
     CDS             complement(4221..4361)
                     /locus_tag="IW819_002365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245452.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcta tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 agtgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACEPW010000183         4371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEPW010000183 DACEPW010000000
VERSION     DACEPW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.188"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1011
                     /locus_tag="IW819_002366"
     CDS             <1..1011
                     /locus_tag="IW819_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245453.1"
                     /translation="SILLSPIINFLYALAIALALTLFGIDALHSPVEVGLIYAFTTYV
                     QGFFNPMSQMMDFLSTFTDGMVAGSRILKIMDTEEYAPQQNPGADQTITSGKIEFRNV
                     SFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEGQILIDDHD
                     IREYPMEELRKKMGLVLQDAFLFYGDIAGNIRMMNPTITDEQIRHAAEFVQANKFIDE
                     LPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTETEGLIQEGL
                     TKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVERGNHETLLAQNGLYADMYKLQSS
                     GV"
     gene            1468..2319
                     /locus_tag="IW819_002367"
     CDS             1468..2319
                     /locus_tag="IW819_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1245454.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2415..4301
                     /locus_tag="IW819_002368"
     CDS             2415..4301
                     /locus_tag="IW819_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1245455.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
BASE COUNT         1376 a          765 c          976 g         1254 t
ORIGIN      
        1 tcgattctgt taagtccgat catcaatttt ctatatgcat tggcgattgc gttggcgttg
       61 actttgtttg ggatcgatgc gctgcattct cccgtagaag ttggactgat ctatgcattt
      121 acgacctatg tccagggatt ttttaatcca atgtctcaaa tgatggactt tttgagcacc
      181 tttacagatg gaatggttgc cggtagtcgt attttgaaaa tcatggatac agaagaatat
      241 gcaccgcaac aaaatcctgg agcagatcaa acgattactt caggtaagat cgagtttcgc
      301 aatgtaagct tttcttatga tggagagcac aatgtattga acaatatctc ttttgtcgct
      361 aatccgggag agaccgttgc attagtgggg cacaccggaa gcggaaaaag ctcgatcatc
      421 aacgtattga tgcggtttta cgaattttat gaaggccaga tcttgattga tgatcatgac
      481 attcgagaat atccaatgga agagttacgt aagaaaatgg gattggtctt gcaagatgcc
      541 ttcttgtttt atggagatat cgcaggaaat atccggatga tgaatccaac tatcacagac
      601 gaacagattc gccatgcagc tgagtttgtt caagcaaaca agttcatcga tgaattacca
      661 aaaggatatc actcaaaagt gatcgaacac ggtgccagct attctagcgg acaacgacag
      721 ctgatcactt ttgcgcgaac aatcgtgaca gatcctaaaa tactagtatt ggatgaagcg
      781 acagcaaata tcgatactga aacagaagga ttgatccaag aaggattgac aaaaatgcga
      841 caaggacgga caacgattgc gattgcccat cggctgtcca cgattcggga tgcagaattg
      901 attttagttt tagacaaagg tcatatagta gaacgcggga atcatgaaac acttctagcg
      961 caaaatggtt tgtatgcaga tatgtacaaa ctgcaaagca gtggtgtgta aaaagtggtt
     1021 gtgacagggc tccgttcagc tttaggtgat aagaaaaaaa ttgtaaaaaa ggctttccag
     1081 atttttgcat aaatttggct tatcaccgat aagctgatac tggaacaccg tatattagaa
     1141 gttgggacag tagcatagta gcattttgac ctaagcgcta gagtgaaaat aaaacagagc
     1201 ttcccagatt cttcatctgt gagcggtagt gtcgtagtca ccagatttaa aaaagattcg
     1261 acagctagtc agaagtgcct aatagccact aagaaagcgt tgacaaaagc gcttgctttc
     1321 gataatataa agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga
     1381 tgagtctccc atggttccat ggacggcttt ttgggttttt ctttttgttt tggatacagc
     1441 cctatatatc atggaaagga gtaaaacatg ctgattgaga aagtactgaa taacaacgtc
     1501 gtaataacca gagatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc
     1561 aaaaagaaag tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca
     1621 aatgacggac tttcagaaca gttccaagaa ttggcacaag cgattcctct tgagtactta
     1681 gaaatttctt gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat
     1741 tcagtgtata ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga
     1801 atatttgtac caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc
     1861 atcggggaaa aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat
     1921 gaagccggat ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccaacaagac
     1981 aatacctacc tgtttactac aacaatacag gatattctaa atatcgtccg ctattactat
     2041 aagattacat ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cctacgtttt
     2101 tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg
     2161 taccaagtta ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac
     2221 tttttagccg agatattcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc
     2281 catactgcgc gtttagtgca tgagaatcgt tgtaaataaa taaaaaagga ggataaatta
     2341 acaagaacgc atgaaataag ggtagtgaca ttcagttggc taaaccttcc actaataaat
     2401 tggagggaat agaaatggga aaatatcaag aattagcgag caaaattgta gaaaatgtcg
     2461 gcgggaagga aaatatcaat ggtttgacac attgtatcac aaggcttcgt ttcaaattaa
     2521 aaaacgaaga aaaagcaaat gatgagatct tgaaaaatat ggacggtgtt gtgacggtaa
     2581 tgcgagcggg aggacagtac caagttgtca tcgggaatca tgtgccagtc gtgtttgaag
     2641 aggttataag agcaggaaac cttacttttg atgaggctgt ttcaacagaa aaaatgagac
     2701 cgtttgatat gcttattgat ggaatatcgg gctgtttcca gccattcctt gctattctag
     2761 ctgctggtgg gatgattcgt ggactgacag cattacttgt cttcttaggc gcatttgacc
     2821 gcggttctgg cacatttgtt atgtttgata atatcggtga ttctgtattc caatttatgc
     2881 ctgttattat cggtttaaca gctgcccgta aatttaaagt aaatgaattc gtagggatgt
     2941 tgcttggggc agcattaatg aatcctagct tgtcgctgga agctttgagt ggtgcagcag
     3001 aagcaccact gacgaccata ttttctggca ctatttttga agcacctatt tatcaaacag
     3061 tattcggcat tccctggatt gcgcgtaact acgcttcttc tgtcattccc attatcttta
     3121 ttgtgttgtt agctagccaa attcagaaac caattaaaaa gttagtacct gagatgatcg
     3181 cgaatttctt cgttccattc ttcaccgttc ttattacaat gcccttaggt tttttactcg
     3241 ttggaccagc attcacattc gcaactgata ttttaatggc tggatttgaa acacttttag
     3301 cactttcgcc agttatttat ggagcaatcg taggcttctt ctggcaaatt ctaatgatgt
     3361 ttggtctcca ttgggcaatc gtaccaatgg gattgatgca attttctgtg aatggctggc
     3421 aaaatatcat gacacctgtt gcagtagtgt cttttggaca aactgcagca cttactgcac
     3481 tctactttaa gcttagaaat ccaaaagaca aagcaattgc aataccagcc attgtttctg
     3541 gtattgtggg gattacagag ccagctattt atgggtttac attgccacgt aaaaaaatat
     3601 ttatttttac ctgtgtcggt ggtgcgttag ggggcgcgta tagtgggtta atgaatttaa
     3661 cttcttggaa tcaaggtgga ctaggcatct ttacaattcc caactatatt agaccagatg
     3721 gtaatttaac agatgtgatt aatgtattga ttgggatcgc cattgcgatg gtggtatcct
     3781 ttgcgctgac ttttttcttt tggaaggagg aagcagatga aacagatgat agacagaaaa
     3841 catcaggtaa agaaattgta aaaacaccga tacaaggaca aatcgctcca ctgaatgcag
     3901 caaaggatgc agcttttgca caaggaacat taggtcgagg aatcttgatc taccctgaaa
     3961 aaggagaagt acgtgcccct tttgacggaa cagtcatgac cctgttcccg acgaaacatg
     4021 caatcggaat ggtttctgaa gcaggtctgg aattgctgat tcatgtcgga ttggatactg
     4081 ttcagttaga aggaaaatat tttaaaagct tggtgcagca aggagaaaga gtgaaacaag
     4141 gcgatctgtt gctccgcttc gacaagaaga aaatcgaaga ggaaggatac agtttagaaa
     4201 cacctgtgat cgtcaccaat tacacggatt atttggatat tttggaaaat caaggggaga
     4261 cggtcgggcc agacgatgca cttatcaccg cactgacctg aacggttagg tgaaagaatg
     4321 gactaggctt aattattctt tttagtccac ttctttattt tcaaaattga a
//
LOCUS       DACEPW010000184         4363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEPW010000184 DACEPW010000000
VERSION     DACEPW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.189"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..340)
                     /locus_tag="IW819_002369"
     CDS             complement(<1..340)
                     /locus_tag="IW819_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245456.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRV"
     gene            complement(340..2091)
                     /locus_tag="IW819_002370"
     CDS             complement(340..2091)
                     /locus_tag="IW819_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1245457.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(2233..2457)
                     /locus_tag="IW819_002371"
     CDS             complement(2233..2457)
                     /locus_tag="IW819_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1245458.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2708..4294
                     /locus_tag="IW819_002372"
     CDS             2708..4294
                     /locus_tag="IW819_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1245459.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         1269 a          888 c          801 g         1405 t
ORIGIN      
        1 ttacccgaac atactgatag gtcttcaatg aagccatgga atacaaatac cattggaaaa
       61 accaaatgat acttttgact atgactccaa acaagtacat accagcaaaa aacaaaataa
      121 cttgtgtcgt agccgactgt ttagccagat gatcatccat aaaagtttgg atgatccttg
      181 gcaataggac attgatcaca gacagagcaa acgcaaataa gatagcaacg atgaacgttc
      241 ttctaaaagg tttggcgaac ttcatcagtc gtttgatgat cttcttctgt tctttaagtg
      301 gaatcgattt tgtccattcc gattgataat tttcatccat caggcctcgc ctccttctat
      361 cttcgcttct aattgctgtt tctcccacat tcgttcatac cagccgccta atgcaagcag
      421 ctcatgatgg gttcctcgtt caatgatttt cccttgatcg acaacaatga tttcttttgc
      481 atgcatcacg ctgcttaaac ggtgagctgc aatgatggtt gttttctctt ttcttatttc
      541 ttttaaatta tgcaagatag cttcttctgt tttggcatct actgcagaaa gtgcatcatc
      601 taagatcagc aattcaggat ttttgatcaa tgctcgagca atcgaaatcc tttgtttctg
      661 tccgccagat aaagagactc cacgttctcc aactaaggta tcataacctt ttggcaattc
      721 cttgatttca ttgttgataa atgctaaaga agccgcatgc tctacttctt cttctgaaag
      781 ttttggatta gaaaaacgga tattatctcg aatggtcatc gaaaacaaga agtgatcttg
      841 aggcacatag ccgattgatc ctaataaagc attcaatgaa tattctttga tcgaacgtcc
      901 gccaaattgg atttttcctt gataatcatc gtattcacgc atcagtaatt tgatgatcgt
      961 cgtttttccc gcaccagttt tcccaacaat acctaatgtt tctccttctt ggatcacaaa
     1021 tttgatttgt tctaatgctg gtgattcttc tttcggatag ttaaaagagg aaatagctac
     1081 attcagctcg ccttttgccg gggtctgcac tgcacccagt tgttccacga tatgcgtctt
     1141 ttcatgcaaa agttcattga cgcgatcata agaagcattt cctctttcca atacgttgaa
     1201 caatcgacca atcgcaaaca tcggccaaac aagcataccg atatagctaa taaatgagac
     1261 catctgcccg atcgagatcg tgccttccaa gacatacttt ccacctaaaa taattgttgc
     1321 aacataagac agaccgataa tcaaggtaat gaatggatca aataaggagt ctaaaaaatt
     1381 gactcgtttg tttttaataa ttgcatcttc aatcttttct tcaaaatccg cgagatcttc
     1441 tttttcttgt ccaaatgttt taatgacttt gattccagta atactttctt gtgtcttatc
     1501 attgatacta gaaaaagctt cttgcgaatc acgaaaagca tcgtggagtt ttgaccccag
     1561 cactcgtgaa gtaacggcta gtaaaggcag aggaatcagc gcaatcagtg ttaagcgcca
     1621 atcaataaac aaaatcatcg caatgatcgt tgttccgccc gtgatcactg aatctgcaaa
     1681 ggtcaagata ccagctcctg ctacattttg gattgcattc aaatcattcg ttgcatgagc
     1741 catcaaatct ccagtccgat atttttggta aaagagattg tccatttctg taaaatgttt
     1801 gaatagctgc tgccgaagtt ctttctccaa ccgagctgca cttccccaaa tattcgtccg
     1861 ccagatataa cgaaaaagat attgtaagag agctgcaagg atcaataata agatccacca
     1921 ggaaatgatc tttattgata aatttttatc ggcaatctca tcaatgatga tcccaatcac
     1981 ctttggagga actaactgta ccaaggccac tgcgaatagg gagaaaatcc caattaaata
     2041 atgtttcttt tcctgtcgaa aaaaccaacc taattttcta aaaatcgaca ttttactcct
     2101 cctttgtctt tatcaagagc ataggattgt ctgtgcttgt cttttttgct gaatagtctg
     2161 aatagtctaa caaattacga aataaaaaac ggactgaaaa aatcagcccg caaaaagtct
     2221 ttgggtcaga acctattttt tcccttggtt cttcatttgc tgcatcatct ggcgaatttt
     2281 cttctcagat ggtttttgcc ccattgacat catcattgat cgcaacatgt cctcattaac
     2341 gggaggattt ttttctaaat agtctttcat atatttacgc gctaggaaaa agccacctac
     2401 tgcgccgatt agcaaagcaa taatagcaat aagtacgaca atacctgttg ataccatata
     2461 aatctctcct ttctatttcc gaattgctct caatgattta ttttactaaa gtttattgaa
     2521 aaaagaaagg cgtattcttt agtttcatgc aaaaaatagt ggttttttta gagaagaaca
     2581 aaatttcttt cattaaccag caagacacgc cacgaaaaaa aattaggcat accgaatatt
     2641 tttctttaat tttttctttc tgaggtgtag aatgtaatcg tagataaata ggaaaggtgg
     2701 ttttttcttg aataatcaat ccaacaaaca atttcataag catcgactta ttgaacatac
     2761 aaatggttta tctttagctg aagtaaatgg aacagtggat gtacccaagg gaaaaagttt
     2821 ttttaaaaca cttcttgctt actctggacc aggtgcatta gtagcagtgg gttatatgga
     2881 tccaggtaac tggtccactt cgatcactgg tggacaaaat ttccaatatt tattgatgtc
     2941 agttattttg atgtctagtc tgattgctat gcttttacaa tatatggcag caaaattagg
     3001 aatcgtgacc caaatggatt tggcccaagc aatccgagca cggacaagta agacattggg
     3061 tgtcatttta tggatcttga ctgaattagc cattatggca acagacatcg cagaagtcat
     3121 tggtgcagca atcgccctct acctgctttt tgatattcca ttagtgattg ccgtatttat
     3181 cactgtgtta gatgttttcg tcctcttatt gctgacaaaa gtcgggtttc gtaaaatcga
     3241 agctttggtt gtctgcttga ttttcgtcat tttattcgtt ttcgtctatc aagttggctt
     3301 gtctaaccct gattggcaca gtttgtttgc cggctttatc cctaacagtc atacattctc
     3361 agactcaccg gtcatcggcg gtgaaactcc gctgactgga gctttaggga ttattggtgc
     3421 aacagtcatg ccgcataact tatatcttca ttcagcagtt tctcaaactc ggaagatcga
     3481 tcaccaagat gaagaagatg tagcacaagc ggtacgcttt tctacttggg attctaatat
     3541 ccagttgtca atggcttttc tagtcaatgc actgctattg atcatgggtg tcgctgtatt
     3601 caaaaccggt tctgtccaag atccttcctt ttttggtctg tatgaagctt tatctgatcc
     3661 atcagctatg agcaatggta tcttgattgc tgtcgcaaaa tctggtatct tgtccacttt
     3721 atttgctgta gcacttcttg cttctggcca gaactctact attactggta cgttgaccgg
     3781 tcaagtcatc atggaaggct tcatccatat gcgtatcccg atttggctaa gaagactagt
     3841 cacacgattg ctttctgtga ttcctgtatt gatttgtgtt ttgtttacaa gtacaaaagg
     3901 aacgatcgaa gaacataccg ctttgaataa cttgatgaat gaatcccaag tttttctggc
     3961 ttttgcattg ccattctcga tgattccttt actgatgatg accaatagcc aagcggagat
     4021 ggggagccga ttcaaaaatt catttgttgt aaaactactt ggctggttct ctgtgattag
     4081 tctgacttac ttgaacttac gaggattacc aggtcaaatc gaaagtttct tcggcgagca
     4141 agccacctca gctcaactag cattggctga tcaaatcgct tatgtaatca ttgttcttgt
     4201 ccttctcctg ttggcatgga cgattggtga tttatataaa ggaaacaaaa aatatgaaca
     4261 gcgtttgcaa gaaacgatag aagaaatcga atagaaactg attcttttac acaaaggagg
     4321 ttgtgacaga agtgggcagc ttcaagaaat aagaaggaat tta
//
LOCUS       DACEPW010000185         4330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEPW010000185 DACEPW010000000
VERSION     DACEPW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.190"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..607)
                     /locus_tag="IW819_002373"
     CDS             complement(77..607)
                     /locus_tag="IW819_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1245460.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            712..1263
                     /locus_tag="IW819_002374"
     CDS             712..1263
                     /locus_tag="IW819_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1245461.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1416..1889)
                     /locus_tag="IW819_002375"
     CDS             complement(1416..1889)
                     /locus_tag="IW819_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1245462.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2027..3499
                     /locus_tag="IW819_002376"
     CDS             2027..3499
                     /locus_tag="IW819_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1245463.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLTKAGDNA"
     gene            3496..4206
                     /locus_tag="IW819_002377"
     CDS             3496..4206
                     /locus_tag="IW819_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1245464.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1357 a          779 c          842 g         1352 t
ORIGIN      
        1 atgaggaact atttttccgt ttctttctct aatactcagg tcagaacgct gatttcacaa
       61 cctctctttc acaaccttat tttactcgtt tataattttc atcgaaatat tttccgctac
      121 ggatatcatc tggcattcca tcgtatggtt cttcgctaat aacatcttca ttgtctatac
      181 ctgcatcacc catataagtg atagtagcaa attcaggaac aagtaatttt ggataagttt
      241 catatttttc acgagctgct tccatttctg cgatatgttc gttttggaaa gtaacagtga
      301 tttctggatg ttcttcaacc catttaggga aaaagatcat tccgtgaagt ttcttttcat
      361 ttggcataaa gtccaatgcc acgtctgtcc ctgttggctc tgtccaagtg attttgaata
      421 cgccgtccgt caatttaacg atatcagctt cttggtcttt tacccaacgg cctttgacca
      481 ttccgccatg gatacggtaa tcaacagtat ggtcattttt ggcataccat tcatattccc
      541 aaccattatc atacgtatag ataaagtgag ttccaataaa atcttccaat tctttaaatt
      601 ctttcatgtt taagcatctc cttataaatt aaaacatgta ataaactaca tgttttcgat
      661 tactcattta tgatagaatg gatttaaaga tttgtcaagg agggaaaact gatggcaaga
      721 aaaaatacac ttcgatatat tttgttaggg ttattaagta agaagaagat gacaggatat
      781 gaattgaatc aatcgttcaa aaatgaaata ggagaatttt ggcaagcaaa acatagtcag
      841 atttatcctg aacttgcaaa aatggaagag caaggaatca ttcagcatca agtagagatc
      901 acaggtgaaa aattggaaaa gaaagtttat gaattaacag aagaagggaa ggaacaattg
      961 gcagagtgga ttcatacacc caccaatgaa ctaccggtta atcgagacga atttgttctg
     1021 aaattgtatt tcgtcaaaga cgtgaatgat ccggcattag tagaaattat tcagcagcag
     1081 cgtgaccttc atgaagaaaa acgacttcat ctattagagc gaaaaaaaac gatctttcct
     1141 actgaaaaag aaaaacatga aaattacgga cactacctga ttttagcgca tgcgatcaat
     1201 cgggaaacag aatatgcaaa ttggctaaca gatgtattag aagaaagtaa ccgaaaaaaa
     1261 taaaaaaata tccgaacagt gatggagaat tcctttctat atgaaagaaa cgcagatcac
     1321 cgttcggata ttcttatgtt caaacaggcc ttttttcaag tatttatgta gcatctactt
     1381 attaaaaagc ttggtactta ggagattagt ggcgtttaga gttcttttaa cagctgttcg
     1441 tagtcagctt tttcatcgac aggaaggagc ttgtaagtta attcgtctgt ctcgttgtca
     1501 tgaatgatgt acatcttagc attctcttct ttgaagttac cgatttgtaa aatcgtatag
     1561 cgttcgtcga tcaattcgat atctttgatt gcttttgaaa aaactttacg gacatggtgc
     1621 attttctttt ggcggatttc tgccttttct gcagcttttg acaaagtaaa tccgtcatct
     1681 aaatattttt tgatcagttc tacttttatg actgtaggta aacgatattt tctaggacaa
     1741 ttcgcatcag cttcgatcga acgaatgaat cccttttgtt cccaatacct taattgacgt
     1801 ggagacacac ctacgatatc acttaattca ctgatgccaa caaccaattg atcattttcc
     1861 aacaaacgtt taagggaagc cccaaccatt tttgtcactc cttttgtcct cttgcttccc
     1921 ttaattatat ccgtattaac ttatgtgtca agtaggttga caaatagcga atttaggact
     1981 aaagtattct agtagtttca gaaaaaattc agaaagaagg agaaatatga gtaacaacca
     2041 acccgtagat atttacggaa aaccatataa tcggacactt ttagttgttg tcttattaat
     2101 tggtacattt tgtaccgttt tgaatcagac cttattgacg acagcttttc ctacattgat
     2161 gaaagctttc gatatttcag cgtctgatgt gcaatggtta acgactggtt tcttgctagt
     2221 caacgggatt atgatcccaa taacagcatg gctgatcaat aaattcagtt caagaaattt
     2281 atatctttct gccatgacga tctttttgat cggaacgatt acttgcttta cagcacctaa
     2341 ttttagtaca ttactaatcg gtcgcttgat ccaagcttgc ggtgtgggag tatcgatgcc
     2401 attattgcaa aatatcatgc tatctatttt cccgccagaa aaaagaggat cagcaatggg
     2461 gatgtcaggg atcgttattg gacttgctcc tgctttaggc cctacattat ctggttatat
     2521 cattgataac taccattggc gtgacttgtt tggtatggta ctgccaatcg ttgttcttgt
     2581 cttggtttta gcattcttcc taatgaagag cgttattcca ttgtcaaatc ctagcattga
     2641 tattttatcc gctattttat caacgattgg attcggtagt ttgctttacg gattttctag
     2701 tgtaggagat gacggctggg gaagcttgaa agtaattggt ttcttagttg ttggtgtgat
     2761 tttcatcgca ttgttttcat ggcgtcaatt acatttggaa catccattct tggaacttcg
     2821 cgtattcaaa tcatctacat tcacgattgc cgctgtttta gctggtgtaa caaatatggc
     2881 gatggtaggg gcagaaatgg tcgtaccatt gtatattcaa aatattcgtg gagaatcagc
     2941 attccattct ggcttgatgt tgttgccggg tgcattgatc atgggggcta tgatgcctgt
     3001 tacgggtgcg attttcgata aatatggcgc gaaacgttta gccatcacag ggatgttcat
     3061 tttgacagcc gcaactgcac catttgcttt cttgacaaaa acgactccag ttttatatat
     3121 cgtgatcctt tatgcgatcc gtatgtttgg gatctccatg gtcatgatgc cggtaacgac
     3181 ttctggtatg aatgcattgc caaataactt gatcagtcac gggacagcgg taaataatac
     3241 attccgtcaa atcgcaagct cgattggtac agctattttg atcagtgtgt tgacgaatgt
     3301 gacaaaagca aatatcccaa gcaaatctgt attggataat acaccattag cttataaaga
     3361 tcaggcaatc aatgccacac tgtcaggtta ccatgccgcg tttatcgtag cgatcatctt
     3421 tgggatcctt ggctttatta ttgccttttt attgaagaaa aaagaacctg ttctaacgaa
     3481 agcgggggat aatgcatgat tattggagtt ttgattgtca gcttgcttgc ttttgcttta
     3541 acgaacgtgt tcgctaaaaa aacatggcaa acctttttat ctttgatttt cggactaatc
     3601 ttcgtcgctt cactaagttt gatcgtcgct aatttgtcca atcactttgg aatggaaaaa
     3661 gtaacggaaa caaaaacaga aaaaattgtt tccagtgctg attcccaagg agcagatatg
     3721 cttttgtata aagctttagg gaatggaaaa gaaaaagtgt atctttaccg aacaaatgaa
     3781 aaacaagaaa aaccaaaagc aactggtacg gataacgaaa caaataaagt agaaaagaca
     3841 gacggcgatg ctgaaaaagt gacgaaaact acttattggg aatacaaaaa cgacatgtat
     3901 aaattctggt tcaatattgc tgataacaat cacgaatatg acagccgagt gaatacattc
     3961 aaaatcccag agacatgggt agaattaagt acggaccaag cagccaagtt agctgaatta
     4021 gttaagaaac agcaaagtac gatggaaagt gaagcaaaag cttatgttca agacggtatg
     4081 gtaaaagcaa tgactgaaaa tccaaaaatg agcaaagcag agcaagaaca acgtacgaaa
     4141 gccttagctg ctgaattcca acaacaagca ttcgcaaaat tagtgaaaga agcaaaaggc
     4201 gaataatatt ttgaacaccg tgtaggaggt tgtgaaaaag ctgctctgca tcaagcagta
     4261 agaacaatca gataaaaata gcactccata tttttcatct gattggggtt agtgccgtag
     4321 atgcaagctt
//
LOCUS       DACEPW010000186         4317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEPW010000186 DACEPW010000000
VERSION     DACEPW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.191"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..188
                     /locus_tag="IW819_002378"
     CDS             <1..188
                     /locus_tag="IW819_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1245465.1"
                     /translation="FKGLIALQEQDFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKML
                     DEERIKQSFDKLLAESK"
     gene            218..340
                     /locus_tag="IW819_002379"
     CDS             218..340
                     /locus_tag="IW819_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1245466.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            358..1881
                     /locus_tag="IW819_002380"
     CDS             358..1881
                     /locus_tag="IW819_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1245467.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            1905..2144
                     /locus_tag="IW819_002381"
     CDS             1905..2144
                     /locus_tag="IW819_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245468.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(2337..2798)
                     /locus_tag="IW819_002382"
     CDS             complement(2337..2798)
                     /locus_tag="IW819_002382"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1245469.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2993..3211)
                     /locus_tag="IW819_002383"
     CDS             complement(2993..3211)
                     /locus_tag="IW819_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1245470.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3329..>4317
                     /locus_tag="IW819_002384"
     CDS             3329..>4317
                     /locus_tag="IW819_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1245471.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIE"
BASE COUNT         1487 a          651 c          861 g         1318 t
ORIGIN      
        1 gttttaaagg attgattgct ttacaagagc aggattttat agttgccatg caagaggtaa
       61 acgaacaggt taattttcaa tacaccactc gacaaaaaga ccaaaccact attattctaa
      121 tcggaaaaat gttggatgaa gaaaggatca aacagagttt tgataaatta ctggcggaaa
      181 gcaaataatg ataagtagtg attgggggta atttctaatg gagaaaacgg atatatctag
      241 tgcatatcga cgacttaaaa gcccgaatat taaaactaga aaaagggcat taaaaattat
      301 caaagaacat aaacgaaata aaatgaagaa attagcataa ttaaggaagg aaaaagaatg
      361 aagaaaatca gtgtaggact tattgggata gcagctctag gattattggg agcttgctcc
      421 agcactaatg atgcaaaagt atcgaatgat aaagatggaa aattagaaat tgtaacaaca
      481 ttctatccaa tgtatgattt caccaaaaac atcgtcggcg atgaagcgaa cgtggattta
      541 atggttccag cgggatcaga acctcatgac tatgagccct cagctaagga tatggcaaaa
      601 gctcatgatg ctgatgtttt tgtatatcat aacgaaaaca tggaatcatg ggtaccaaaa
      661 gctaaagaaa gttggaagaa agctgggcct aatgtagtag aaggaacaaa agacatgatt
      721 ttattgccag gtagtgaaga agaagatcat gatcacggag aagaagatca ccatcatgaa
      781 ctagatcctc atacgtgggt ttcacccaaa atggcaatta aggaagtttc aaatatcaag
      841 gatcagttag ttaaattgta tccgaaaaaa gcaaaagtat ttgagacaaa tgctgaaaag
      901 tatttgacta aactgaaacg gttggatgct gactatacca ctagtcttaa agaagcgaaa
      961 caaaaaagtt ttgtgacgca acacgcagcc tttggatatt tagctttgga ttatggattg
     1021 aaacaagttc ctattgctgg actaagtcct gaagaagagc catcttctgg tcgattagct
     1081 gaattaaaag agtatgttaa aaaaaataag atcaattata tttactttga aaagaatgct
     1141 aatgataaaa ttgcaaaaac tttggccaac gaagctggga taaaattgga agtactgaat
     1201 cctcttgaaa gtcttacaaa agaacaaatg gataatggtg aagattatgt ttcagtaatg
     1261 gaagacaatc taaaagcgtt agaaaaaaca actatggttg ctggtgaaga ggtagttcca
     1321 gaaaaagaag cgaaggatga aaagacagtt gctaatgggt actttaaaga tgccgatgtg
     1381 aaggatcgcg aattgagtga ttatacaggc gaatggcaat ctgtttatcc gttgttaaaa
     1441 gatggaattt tggatgaagt tttcgattac aaagcaaaaa tcaataaaga catgacagct
     1501 gccgagtaca aagattatta tactactggc tataagacag atattgatac tatcaacatt
     1561 aaagacaaca ccattgattt tgtagtcaac ggtgaacatc atcaatacac atacaaatat
     1621 gttggataca aaattttgaa ttatgaaaaa ggaaaccgtg gtgttcgttt taattttgaa
     1681 actgatgatg caggtgctgg tcgttttaaa tatattcaat ttagtgatca tggaattgca
     1741 ccaagtaagg cagagcattt ccatattttc tttggtggag aaagtcaaga aaaactctat
     1801 aatgaattgc ataattggcc tactttctat ccagcttcat tgtctaaaca tgaaattgct
     1861 caagaaatga tggctcatta aaattcaatt tttggaggaa atacatggta agaaaagtat
     1921 tattgtctat ggattccaag aatattgatg cttatcggcg ttatcgattt gcaaagcaaa
     1981 tgagattaaa tgggagtaca ttagctgata ttgctgcaac tttacatgtt agtattgaca
     2041 cagttcatag agtattaaaa cgggatcctg attgttgttt tatcaaggtc aatactggaa
     2101 gaagtagacg tttagcaaaa cggcataata gcttggccaa ttaattgata ataccagagt
     2161 agatcatttt tcccttattt ggatttgatc tgctttttat tatgtcttga attgtcaaat
     2221 caagcgcaac actttatcaa agaagacttc ataaggtgtt ctccaatcta aacactttct
     2281 aggtctgtta ttcagttttt caatcatctg atttatcgtt gagttatcta ccgtagctat
     2341 ctctttcgat tttggcaagt attctcgaat gagtctattc gtattttaat ttgttcattg
     2401 ttgccaaggg catgaggatc tggaaaataa aagggcatat tattcaatgc ttctgtaact
     2461 gcgaaatgat tggagaattc ttttcctcta tctggagtga tcgttttgac cgcctcattt
     2521 ggaatactgc ttaaaagttc aatcattttc tcgctcacat aagcggcttt ctttctgggg
     2581 accttctttc ctgctaaaag gaagcatgat tttctgtctg tcaaagtcac taaacaagta
     2641 ccaccagttt tgccagctac aatatcggct ttccaatgac caaaatcact tctatcatct
     2701 gcggattttg gacggccatg gatcgaatga gtaaggcaaa tctttccacg agactcgaca
     2761 tgattttttg tatgtcttgt ttttcctcga tggtgcaatg aacggatcag gcctttattg
     2821 ccgtgtgaga ggggttctgt atcgaaaaga tcattgcaaa ttgcacgtta gatcgtgata
     2881 aaactaattg aaaagtccat atgttcatgc ttcaaacgat tcgagatttc ctctggcgac
     2941 cactgaaaat cgacaaataa atgacgaaca attgaccaaa ctcacggatc atttagaatg
     3001 tgataacgtc cgcatttttt tcttcgataa gcataatttt tttccgcttt ggaaggtgaa
     3061 tagttttttt cttgtttctt ctcagttcat gtaaaatagt aaacgggcta tgctgaatgt
     3121 cgttgccaat tatactaata gaaaatcctt cttcgtgaag cagaaagatt ctttctcgtt
     3181 cgagtatagt aagatgacga tactggctca taattatttt ccttctagga gtgttctcga
     3241 caaacttata ctagttggat atcgattatg actcattttt atgtgttgca cttaaagtgt
     3301 aaattcaagg tatagaaaga ggttccctat gaaaaaaata cctattacaa tcttaactgg
     3361 ctatcttggt tcaggaaaaa cgatacttct aaatcatatt ctgtacggtg atcacggaaa
     3421 ggaaattgct gtcattgtaa atgacatagg ggaagtaaat gttgatgggg tattagtcga
     3481 caagaaaggt tttaaaagaa ctggagaaaa acttgtttcg atgccgaatg gatgcatttg
     3541 ttgtacatta cgcgaagatt tgattgttga actagatcaa ttagtgcaat tttctgactt
     3601 gaatcaaatt gttattgaag cttcaggaat cagcaaacca attccaatta ttcaagcaat
     3661 tatattagct aaaaaccctt tagacattga tttgacccaa cgattagaga ttggtgccat
     3721 tattatagta gtggatgtaa aacgtttttg ggataatttc gaaacgggtg aaaagattca
     3781 ggaaagaaaa gctgaaggta atgtagttga agaagaacag gatattcgtg gcttgttaat
     3841 tgatcaattc gagtgttgta acatattact tttaaataat agtgacttag tgtcaaccaa
     3901 gacgatttac aaaattaacg gtctaattgc taagttacag tccaatgcca aaataattag
     3961 gacaattaaa ggtgaagtag acttatcgaa aattctaaat acaaatcttt ttaatataga
     4021 tgagacttcc tattcagtgg gctggttcaa agagttagag cttggctatc aaaatcatat
     4081 tcctgaaaca gaagaatatg gtatctcaag ttttatctat cgaaatcggt tactccttga
     4141 cgcacaaaaa tttgcccatc ttacggataa aaattttcct ggcaatatta caagagcaaa
     4201 agggtttctt tggtttgccc aacatagtga tttttcgacg atctttgaaa catcgggtag
     4261 ctataaaagt attgaatcat taaattattg ggtagctagt ttacctaaaa ttgagtg
//
LOCUS       DACEPW010000187         4308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEPW010000187 DACEPW010000000
VERSION     DACEPW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.192"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            104..1078
                     /locus_tag="IW819_002385"
     CDS             104..1078
                     /locus_tag="IW819_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1245472.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1060..1521
                     /locus_tag="IW819_002386"
     CDS             1060..1521
                     /locus_tag="IW819_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1245473.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1571..2035
                     /locus_tag="IW819_002387"
     CDS             1571..2035
                     /locus_tag="IW819_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1245474.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2049..2798
                     /locus_tag="IW819_002388"
     CDS             2049..2798
                     /locus_tag="IW819_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1245475.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2798..3628
                     /locus_tag="IW819_002389"
     CDS             2798..3628
                     /locus_tag="IW819_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1245476.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3628..4275
                     /locus_tag="IW819_002390"
     CDS             3628..4275
                     /locus_tag="IW819_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1245477.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
BASE COUNT         1478 a          556 c          825 g         1449 t
ORIGIN      
        1 ttttttttga aaaatagtat tgcaaaataa agcaatttca tagataatag gtatcgtaaa
       61 gttatataac tttataatac ctattagagt gagggataaa gaaatgagac aactacacaa
      121 agaacagatt gaaacagcaa tggcagcgtt aaaggaaaag acaattgatc atgtatattt
      181 tacagcatgt gggggttcac aagccgtttt aatggctggt caatatctat ttgataaaga
      241 attaactgta ccttgtcatg tttatactgc taacgaattt aactatgcta taccaaaagg
      301 attatctgaa aattctttag ttatcacttg ttcccattct ggcacaacac cagaaactgt
      361 agaagcaaca aaaaaagcaa gagaatttgg agcaatgact atttgtttat ctaatgaaga
      421 aggatctcct ctatgggaag cagctgaata tcctgttcac tatgattggg gtaaagatgt
      481 tgatgctagt gatttaaata aaggaattct ttatggttta ttatttaata ttttacttgt
      541 tttagatggc tctaaaaaat gggaaacttg cttgaatgaa tttgataagt taaatgatct
      601 tgttattgct gaaaagagca aatttgaaat aactgctaaa gaatggggta aaaaccaaaa
      661 acgagataaa attatttaca caattggtag cggtattaat tacggagaaa tttactccac
      721 agcagcttgc tggttcatgg aaatgcaatg gattaattct aatgctattc attctgggga
      781 atttttccat ggtccttttg aaatcactga ttatgatgta ccattcatct tagtgaaaag
      841 tattggagat acaagatttt tagatcaacg ggtagaagac ttcactaaaa aattcagtaa
      901 taaattaatg gttttagacc aagctgattt tgacttagaa tctatagata gtgtagcgaa
      961 agaatatatt gcagctgtac ttagtggcgt tgttattcgc caaatggtag aagcgattgc
     1021 ttttgaaaga ggccattcct tagatgttag acgctatatg tggcaaatga aatattaatt
     1081 aataggaggt tgcaaaagat ggcaaatcaa ttatttcaca ttgtgattac aacacattct
     1141 accctttgtg agggttattt aaaggcaaca aaattaattt taatgaatga cgccacaggt
     1201 attagtgctg ttccatttaa agaaaatact agtccagatg attttgagga gagtatgaaa
     1261 gaaataatta gtcagcatca tttgcaacct ctaattattt taacggattt aattggagga
     1321 acacctaata atgtttctat taaacagttg aacaacaata gaattcaaat tattgctggg
     1381 attaatttac ctcttttact ggagttatta atggcccagt cagctggtaa atctatagaa
     1441 gaaatagatt ttaaggaatt actgaataac tctcaaaaaa gtatggttca tattaattcg
     1501 cttttaaaag atgtcagtta aggtggcaat ttctattcta taaattttaa aaattgaagg
     1561 aggaaataga atgattaaat tagtgcgtgt agaccacaga ttacttcatg gacaagtaat
     1621 attttcttgg actaaacagt atgatattaa ccatattatt gtggctgata acaaagttcc
     1681 aggagatcca atgtctgtga tggctttgac aattgctaaa cctgctgatt gcaaattaga
     1741 cattgttaaa attagtgatg ttaaagggat tatggaaaca tccccaaatg ataattacat
     1801 gattttaatt aaaggaccta aggaagcttt tgaattagtt caaaatttac cagaaattaa
     1861 agaaattaat tttggcggtg tgtctaagaa aagcgagtcc aaacaatatg gaaaggcagt
     1921 ctttttaact cctgaagaat tagaagccac aaaaaaaatt cttgatcaag aagtgaaaat
     1981 atttgtgcaa caagtcccat cttcaagtat tgaaaatgta gattttagta agtaaggggg
     2041 gaaaataaat gttattaaaa gccattttat taggtttagt tggtgtggta gctgttattg
     2101 actccaggtt aattggtcga caaaatattg gtagaccact aattttgagt acacttgccg
     2161 gacttgtgct aggtgacttg catacaggga ttgttcttgg ggcatcttta gaattaattt
     2221 ctatgggatt cgtctcaatt ggggcggctg gacctcctaa tatgcaactt ggtagtatta
     2281 ttgccactgc ttttgcaatt ttaactaata gtagtacaga aacagcctta acaatagcta
     2341 tcccagtggc tgtagctggt gaatttatga gtattatcat gcgtatgata attgctcaat
     2401 ttggacatgc tgcagataaa gccattgaaa ataataaatt tagaaaagct caaattattc
     2461 atatttattg gtcatttatt tttaatgctt ttgtttactt tattccaatc tttttaacag
     2521 tatattttgg tgctgacgtt gtggcagatt tagtagataa aattccagaa atgattacag
     2581 acgccttgac tgtctctgga aatatgcttt ccgcattagg gtttgcaatg ctattaagca
     2641 caatgttgag taaaaaatta tatccttact ttatttttgg attctttatt gttgcctatt
     2701 caggtctttc actaattggc gtgacaattt ttgcagctct gattgccttt gtaatggatc
     2761 aagttaaata tggaaaaaga gaagaggcgc atggttaatg gaaaatagta cagaaaaaaa
     2821 acgtttaatc acaaaaaaag atcttcataa gatgtgtctt cgttcatgga caatcatgtg
     2881 ttcgtggaat tatgaaagac aaatgcatat gggatttatg tatgggatgg cgccaatttt
     2941 aaataaagtt tataaaaatg atgaagagaa aaaaagagaa gcttatactc gtcatatggc
     3001 attttttaac tgtacgcctc aaatgactcc atttattatg ggactttgtg cttctatgga
     3061 agaacaaaat gctcaagcag aagaaggaaa ctttaatact gaatctatct ccatgattaa
     3121 aaccagttta atgggtcctt ttgctggaat tggtgattca tttttccaag gtactttaag
     3181 aattattaca tttgggattg gattgtcttt tgctaagcaa ggtagtattt taggaccgat
     3241 tttagctgtt ggcctttttg ctataccttc tattttagct gttttttacg gcggtaaatt
     3301 aggatatgag tctggcaata aataccttac taaattgtat caagaaggtc ttatggatcg
     3361 agtaatgcaa tttgcttcta tcattggttt ggctgtagta ggtggaatgg tggctagcat
     3421 ggtgccaatt gtcacccctt taaaattcac tacaggggga acagaacttg ttttacaaga
     3481 cttacttgat tccattattc ctggattatt accacttgta tgtactttgg gaatatataa
     3541 attgattaag aaaaatgtta gtacaaatat gctcttatta ggttgtattt tggcagggtt
     3601 tgttttaagt ataataggta tcttataatg attaaggctc taatttttga tatggacgga
     3661 gttttaattg atagcgagat tatgtatcaa aagtgggtat ttaatttttt aaaacaagaa
     3721 aatttaagct atcctttgta ttcttatcaa aaaaagattg gaactacagc aagtattttt
     3781 gatgatatag aaaagtacaa tgagggatgt aacagtttag aattgaaggg aaaatttact
     3841 aaatattgga aatcgcaaaa gattgattac ttggatattt ttcctaatat actaattgaa
     3901 cagcttgaat ggttaaaaaa gaaaaaaata aaaatggcaa ttgcttcttc cagtccaatg
     3961 gatactataa atgaagttgt taccacttgt aaaatagagt catattttga ttgcttaatt
     4021 agcggaagag atttgccaga aagtaaacct aatcctacta tattccttaa agcttcagaa
     4081 cagctcagaa ttccagttga agaatgtctg gtgatagaag attcttataa tggtataaaa
     4141 gctggtaaaa gagcaaatat gaaggttctt gcaattaaag ataaacgttt ttctcaagat
     4201 gtaagtctag cagatgatgt tatttacgat attaaatatc tgcgacaaga agtacaggtg
     4261 aaatttggaa tataaataag ttttttgatg tatttagaga atattagt
//
LOCUS       DACEPW010000188         4269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEPW010000188 DACEPW010000000
VERSION     DACEPW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.193"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(213..443)
                     /locus_tag="IW819_002391"
     CDS             complement(213..443)
                     /locus_tag="IW819_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1245478.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="IW819_002392"
     CDS             complement(673..1491)
                     /locus_tag="IW819_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1245479.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="IW819_002393"
     CDS             1652..2341
                     /locus_tag="IW819_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1245480.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="IW819_002394"
     CDS             2355..3857
                     /locus_tag="IW819_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1245481.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..>4269
                     /locus_tag="IW819_002395"
     CDS             3870..>4269
                     /locus_tag="IW819_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1245482.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPE"
BASE COUNT         1416 a          623 c          757 g         1473 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggaga
//
LOCUS       DACEPW010000189         4232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEPW010000189 DACEPW010000000
VERSION     DACEPW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.194"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1436
                     /locus_tag="IW819_002396"
     CDS             <1..1436
                     /locus_tag="IW819_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1245483.1"
                     /translation="ILAYLDRQIVKNPASPSSSFVREAYQDSYKRFIQPAIERELRNE
                     LTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTGCKLAVVDATGKVLAIEV
                     IYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRESELFVAEQLKSADHKTY
                     YAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQDPLAELVKIDPKAVGVGQ
                     YQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHISGLNKTTAQNIVIYREEN
                     GEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGIHPESYSIAKEILTSVQL
                     SEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLTQPGRDMRDEMPAPLLRT
                     DVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHISKLSKKFVKHPTDVVSVG
                     DIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            1456..1938
                     /locus_tag="IW819_002397"
     CDS             1456..1938
                     /locus_tag="IW819_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1245484.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            1954..2715
                     /locus_tag="IW819_002398"
     CDS             1954..2715
                     /locus_tag="IW819_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1245485.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            2846..4180
                     /locus_tag="IW819_002399"
     CDS             2846..4180
                     /locus_tag="IW819_002399"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1245486.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT         1419 a          764 c          918 g         1131 t
ORIGIN      
        1 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
       61 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
      121 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
      181 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
      241 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
      301 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
      361 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
      421 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
      481 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
      541 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
      601 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
      661 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
      721 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
      781 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
      841 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
      901 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
      961 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     1021 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     1081 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     1141 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     1201 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     1261 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     1321 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     1381 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     1441 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     1501 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     1561 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     1621 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     1681 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     1741 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     1801 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     1861 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     1921 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     1981 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     2041 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     2101 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     2161 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     2221 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     2281 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     2341 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     2401 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     2461 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     2521 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     2581 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     2641 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     2701 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     2761 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     2821 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     2881 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     2941 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     3001 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     3061 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     3121 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     3181 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     3241 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     3301 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     3361 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     3421 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     3481 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     3541 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     3601 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     3661 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     3721 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     3781 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     3841 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     3901 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     3961 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     4021 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     4081 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     4141 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     4201 tataaaaata gaaagagggg gagtggcaaa aa
//
LOCUS       DACEPW010000190         4220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEPW010000190 DACEPW010000000
VERSION     DACEPW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.195"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..758)
                     /gene="eno"
                     /locus_tag="IW819_002400"
     CDS             complement(<1..758)
                     /gene="eno"
                     /locus_tag="IW819_002400"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1245487.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKE"
     gene            complement(874..1629)
                     /locus_tag="IW819_002401"
     CDS             complement(874..1629)
                     /locus_tag="IW819_002401"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1245488.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     NETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(1733..2923)
                     /locus_tag="IW819_002402"
     CDS             complement(1733..2923)
                     /locus_tag="IW819_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1245489.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3033..4034)
                     /gene="gap"
                     /locus_tag="IW819_002403"
     CDS             complement(3033..4034)
                     /gene="gap"
                     /locus_tag="IW819_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1245490.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4075..>4220)
                     /locus_tag="IW819_002404"
     CDS             complement(4075..>4220)
                     /locus_tag="IW819_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1245491.1"
                     /translation="KVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEAAANEILKG
                     VTL"
BASE COUNT         1082 a          889 c          798 g         1451 t
ORIGIN      
        1 ttttctttgt cgtagaattc tgaagaagca gcatccatag caagaacaac gtctttacca
       61 ggtacatagc cagctttttc gattgcttcg atgattactt caaaaccttc ttcgtttgaa
      121 ccaaggtttg gagcaaatcc accttcgtca ccaacagaag ttgctaatcc gcgagctttc
      181 aagattgaag ctaatgcgtg gaatacttca gcacccatac gtagagcttc tttgaatgtt
      241 ggagcgccaa caggcatgat catgaattct tggaagtcga ttgagttatc agcatgtgat
      301 ccgccgttga tgatgttcat cattggtgtt ggcaatactt ttgtgttgaa tccgcctagg
      361 tagtggtata aaggtacttc taggtagtca gcagctgcac gtgcaacagc gatagaaaca
      421 cctaaaatag cgttcgcacc taatttacct ttgttaggag ttccgtctaa agcgatcata
      481 gctttgtcga tagccatttg gtcacgaaca tcgtagccaa tgattgcttc agcaatgatg
      541 ttgtttacgt tgtcgacagc tttagttaca cctttaccgc cataacgagc tttgtctccg
      601 tcacgtaatt caactgcttc gtattcacca gttgaagcac ctgaaggaac cattccgcgg
      661 ccaaaagctc cgctttctgt gtatacttct acttcgattg ttgggttacc acgtgagtct
      721 aagatctcgc gagcgtaaac atcagtaata attgacatgt tttgtctctc ctttgagttt
      781 gttttttcct aagggatttc cctcatatac gattttagtt aattttgctc attagtgcaa
      841 acatttcttg catttttcgc aaattttcaa caattatttt acagcgtcta acaatgacaa
      901 aaatgaatct gcttgcaagc ttgcgccacc gactaaagca ccgtcaacat tttcttttgc
      961 catgtattca gcaatatttt ctggttttac agaaccgccg tattggatac gaactgcttc
     1021 tgatacttct tttccataaa gtttttcaac tgtttgacgt acaactccac aaatttcgtc
     1081 agcgatttgt gcgtcagctg atttaccagt accgattgcc cagattggtt cataagcaat
     1141 aaccatgttt gacacttgtt cagctgaaag atcagctaaa ccagctgtaa tttgtccttc
     1201 gatccattca gcagttttgc ctgcttcata ggtttctaaa gattcgccgc agcataggat
     1261 tggtgtcata ccatttgcaa agatagcttt tgctttttta ttgatgtctt cgtctgtttc
     1321 attgaaatat tcacgacgtt ctgaatgtcc gatgatcaca taatgaacac ctagatcagc
     1381 aagagctgct ggtgaagttt caccagtgaa cgcacctgag ttttcccagt agcagttttg
     1441 tgctgagatt tttagttctg taccttcagc tgatgtcatt aattcttgta agaataatgc
     1501 aggagatccg atgactgaat caactttgtc atttgaaggg attttgtttt ttactgcttc
     1561 agcaaattct tttgcttctg atgcagtttt gttcattttc cagttacctg cgataatagg
     1621 tttacgcatt gaggaacaca ctccttaatt tattgaaaaa tggctaggga gtcatgaaaa
     1681 ctcccttcac cttttgatcg attactcgtc agaattgagc aaacttggtc gtttatttat
     1741 cgttgattgc tgctagtcct ggtaattctt taccttcaag caattccaag cttgcgccac
     1801 cacctgttga gatgtgtgtg aatttgtcag caaatcctaa ttgttctgct gctgcagctg
     1861 agtctccgcc accgatgatt gttgttgcgc cttctaagtt agcgattgct tcacaaacac
     1921 cgattgtacc tttagcaaag ttactcattt caaatacacc cataggtccg ttccaaacaa
     1981 ctgtttttgc accttgtaat gtatcagcaa ataattttac tgatgctggg ccgatgtcta
     2041 aagccatcaa accatcagga atgtctcctt cgattacttg tgtttcaaca tcatttgaaa
     2101 attctggtgc acagacattg tcgattggca agacaagttt gtcgcctgct ttttcgatta
     2161 attcttttgc tagttccact ttatcagctt ctactagaga gttaccgatc ttgattcctt
     2221 ttgcttcgta gaatgtgtaa gtcattccgc caccgatcaa gattttatct gctttaggaa
     2281 tcaagttttc gattacgccg attttatcag aaacttttgc gccaccaagg attgcaatca
     2341 ttggtcgttt tggttcttct actgcttcac cgatgaactt gatttctttt tccatcaaga
     2401 atccagcaac tgttgggata ccagttgaag cgatacctac gttagaagca tgtgcacggt
     2461 gagctgtacc gaaagcatcg ttaacaaata catcgcctaa tgaagcccag tatttaccta
     2521 gttctgtatc atttttgctt tcttttttac cgtcaatatc ttcaaatcgt gtattttcga
     2581 atacgacaac gtcgccgtca ttcatgttgc ggattgcttc ttctaattgt tctccgcgag
     2641 tttcaggtac gaatgttact tctttaccta ataattcgcc tagacgtttt gcaactggag
     2701 ctaaagattt tccttcttta tcttcttctg tttttacacg tccaaggtga gagaaaagga
     2761 ttgcttttcc gccttgttct agaacgtagt tgattgttgg caatgctgct ttgatacgag
     2821 tgtcgtcagt gatcacgccg tctttcaaag gaacgttgaa gtcaacacgg acaagaactt
     2881 ttttgtcttt taaatcgata tctttcactg ttttcttagc cattttattt cctcctgaat
     2941 aaacttatta tcaaaaaaag cggaagaagc gcgattgctt ccccgctttt aaatgtacat
     3001 gtctataatt tacacttgta cgtttgtcta tcttataagt tagcgaagta ttctaaagta
     3061 cgtactaatt gtgcagtgta tgacatttcg ttgtcgtacc aagcaacagt tttaactaat
     3121 tgtttgtcgc caactgtcat tactttagtt tgtgtagcat caaataatga accgtaagtc
     3181 ataccaacga tatctgaaga aacgatttcg tctgtgttgt atccgtaaga ttcgtttgaa
     3241 gcttctttca ttgctgcatt gatttcgtca acagttactt ctttttcaag aactgttact
     3301 aattctgtta atgaacctgt tggtacagga acacgttgag cagctccgtc taatttaccg
     3361 ttcaattcag gaattactaa accgatagct ttagcagcac ctgttgagtt aggaacgatg
     3421 tttgcagcag cagcgcgtgc acggcggaag tcacctttag gatgaggtcc gtctagagtc
     3481 atttggtcac ctgtgtaagc gtggatagtt gtcattaatc cttcaacaac accaaatttg
     3541 tcgttcaatg ttttagccat aggagctaaa cagttagtag tacatgaagc acctgagata
     3601 actgtttctt taccagttaa tgtttcgtgg tttgtgttat aaacgattgt tggaacatcg
     3661 tttccgccag gagcagagat aacaacgcgt ttagcaccag ctgttaaatg tttttcagct
     3721 gctgatttag aagtgaagaa accagtacat tctagaacga tatctacgcc tagttcgccc
     3781 catggtaatt cttctgggtt gcggttagct aatactttaa cttcttttcc gtttacgttg
     3841 aatgaacctt catgaacttc aactgttccg ttgaaacgtc cttgagttgt gtcgtatttt
     3901 aacaagtgtg ccaacatttt agcatctgtc aagtcgttga ttgctactac ttcgattcca
     3961 tctacatctt ggatacgacg gaatgctaga cgtccaatac gtccaaaacc attaatacct
     4021 actttaactg tcatttaaga tttcctcctt atgaaaatca aaaaaatatt tattttaaag
     4081 ggttacccct tttaaaatct catttgcagc agcttcatca gtgatgagcc acgtttgttt
     4141 tggtgcattt ttcatataag ctcgaattgc tttagctttt gattttccgc ccgcgattgc
     4201 catgatgatc gggacttttt
//
LOCUS       DACEPW010000191         4200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEPW010000191 DACEPW010000000
VERSION     DACEPW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.196"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..1426)
                     /locus_tag="IW819_002405"
     CDS             complement(26..1426)
                     /locus_tag="IW819_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ1245492.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(1430..1840)
                     /locus_tag="IW819_002406"
     CDS             complement(1430..1840)
                     /locus_tag="IW819_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1245493.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1858..2349)
                     /locus_tag="IW819_002407"
     CDS             complement(1858..2349)
                     /locus_tag="IW819_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1245494.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(2359..3150)
                     /locus_tag="IW819_002408"
     CDS             complement(2359..3150)
                     /locus_tag="IW819_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1245495.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3143..3916)
                     /locus_tag="IW819_002409"
     CDS             complement(3143..3916)
                     /locus_tag="IW819_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1245496.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1316 a          868 c          562 g         1454 t
ORIGIN      
        1 cacttacatc atcccttcaa ttgttttaaa atccacattc ataatttatt gaatagtcta
       61 gtttatctac cttgattgct ccctccgtaa cagtcatact taatgctgtt ccgactttat
      121 catacattct agtacttaaa gcaacattat caatatataa aacagctatc gtatcatcaa
      181 caattaactt tagttcatat tctttgccta tttccaaaaa tagtggtcgt tgcagcccct
      241 tatcaaacat ttgaggccaa ggatagttcg gagtttttcc gaaagtaagc tgattattaa
      301 tcaatgaaat acgaaattcg tatccttctt gagtatcact atctacaaat aattttaaag
      361 caaactcctt tgttaacgat gtaatagtca aagttgtttc aaatagaaaa taatcttcga
      421 ttttaggaac aattactctt tctttttttc caaattcttt ctctaactta aaacttgaaa
      481 atggagtgat ttctttaaaa ttctcaatta ttttttcagg cagtttaaca ccaagagttt
      541 tgtctgtctt ttgaataatt tgatgtggaa caagtgcacc gccccaatcc cagttatttt
      601 gatcgttctc cccttctttt gttgccaccc agccagttag aaaacggcca gtttcattag
      661 ttccgactgt tcttgctgca taataggctt ttccgtcgaa agcttcgtct actggtgatt
      721 tccaatctga aaataatcct gctccgactc gatatttagt ctttttatct tcacaatact
      781 ctgaaaaaag taaataccac gttccatcaa tttcaaatat atcaggcatt tcaatcatgt
      841 taaattcatt attagcccag tagtctcctt tgaaattcca atcttttaaa tcttcggatt
      901 caaaatagac caatctcccc gtcggctttt tcttacttgc tggttttcta gctcctagta
      961 tcaaaacgta ttttccatga tcttgatcaa aaattgcaaa aggatcgcgc caatcattct
     1021 tatcatatcc ttcttgagga acaagttttg ctgcttcgcc atcctttttc caatgaatta
     1081 aatctttaga acttgcaatc attaacactt ctgaagtttt tccatccaac ttagctttgc
     1141 gattatgtcc agtatacaaa gctaaatact cattgattcc ttggacaata cttccagcgt
     1201 aaatgtattg atcaatgtca tcgttgccac cacgatgaat tacttccccg tattcttcaa
     1261 atttcacaaa atcagtggtt gtaactagtg accacccaaa aggatcagtt aaaggtttag
     1321 gatttctcgt atctcgttga tgaaatagat aaaaagttcc atctttataa aaaggcatgc
     1381 aatccccaaa ccaaaaatct ttaggcttgt aaaaaatttt ttgcatttat cactcatcct
     1441 cttcgttagt atgtctttgc atatctgcaa gacacgcaat tgtattcata tttcttagat
     1501 tttcgatgat atcatcgctt ttttttcctt gtaatctcaa attgacagcc tcaatcaaaa
     1561 ttcctaaatt catgccacca agaagttgaa acccattttg aaatacttca cttcttttag
     1621 caaattcgtt gaaaggtgtt ccaccaatca agtcagcaaa taccataact tcttgatttt
     1681 ttacttgaga aactattttt tcaatgtttt cacggaattc ttcaatgcct gcatctccaa
     1741 gtgttaccac atgacaattt aaattatcgc caaaaaacat ttttaatgta tctacaaatc
     1801 caatggcaat atttccatga gatgtaatta taaactccat ccactcatcc tctgttctta
     1861 ataaataaaa tcctttaaaa attcttttga atctctagga gtttgtatca ctgtcacttc
     1921 aactccatca ctaattagtt ctttaaaagc ctctctctct tcttcagtaa tatttatcgt
     1981 cggacggatg cgttctgttc cattccttct tgataaattt cctacgttta cacttgttat
     2041 tggtaaaccg ttatcaatca aaaatttaac atcgattgga gaaactgcaa caataagaac
     2101 tttttgtcct tcatatttac cagaaagaat atttttaact gctacttccc ttgtaattat
     2161 tgaagttcta acattaggtg gtgcagcgag gcgaactaca gcttttttta cgtcatcatt
     2221 ggctacttca ttattaataa ccataattct gttagcactc aattgatttg tccattgtgt
     2281 tgctacttgt ccatgaatca ttcgatcatc aatacgaaca tgtataatac ctttcatttt
     2341 ttcatccata ttcaacacct acttcaaaat atttaacgct cttaacgcaa tagaaactac
     2401 taagattaat agaattacat aaactggttt aacctttcgt cctaacaaat aatacacaat
     2461 tgctactaca catgcaggta ataaaccagg cataacttga tcaaagattt catttccggt
     2521 cattgttaag tcgccttgtt taaattcaaa gccaacattc acttttacaa ctgtggcaat
     2581 gagtgcaccg accaccatta accctaaaat tgatactgca tcagtaatag actttaattt
     2641 tgtttcaatt tgagttagta ggctcgcacc cgattctttc ccagcaaggt ataaaggata
     2701 agcagtaatc cttaataaaa ttgatgccag tatccacaaa ataattccaa caaaactacc
     2761 ttccaatgcc atattagctg caattgctcc aaaaatagtc catggaataa ctacaaatac
     2821 actatctcca attcccgcta aaggacccat tagacctgtt ttcagagcac ttactgcttc
     2881 caaagactta tttttttctt ctttttcaat tccaacattc ataccaataa tgtaaggcgc
     2941 tgtatacggc gtagtgttaa aaaattgtag gtgagctaaa acggattgtt tcattccttc
     3001 aggatcatct ttatatgtgt ccttgataaa aggaagcata gaatacgcat aacccaaccc
     3061 ttccattttt tcataattta atgaagttgc accattgaga atccaccgga aaattacttg
     3121 tttgtcgatt tttgtcagtt tcttattcat cttcgtcaaa acctcctgtt gcagctggtg
     3181 ctacttgagt attatttgat ctatttttat atacgattag tgcgattgca gcgccaagta
     3241 acgaaatccc taaaattggt atttggaaat aaactgccaa aacgaaacca actaacaaat
     3301 aggcaaagta atcttttgta ggtaaatatt taagtagcat tcctatccca acagcaggta
     3361 atagtccccc tgctaccttt aaaccaccgg taacccattc aggaatataa ttgattgcac
     3421 ttcccactaa cgttgggcct aatatcacag ttaacaaaac aggaattcca gatacaaagc
     3481 ttgtattaat tgatgccaaa tattgcatcc actctaattt ttgataatct tctttttgtg
     3541 cgaatttttc tgccttttgt tgaaaaaaaa tgtttgttga ccactttaga acatctagtt
     3601 gtacaattaa cattgctacc ggtattgcta aagttattcc tatagaagca tcctggttag
     3661 ttgagattgc taaataagta ccgatcaatg ctgctgtttg ataatccggt actgatgcac
     3721 caccaaaact agtaatacct agtgtcatta attgcaaagt cccaccgata taaagtccag
     3781 tttgaatatc tcccataata agtccggtca agaaacctgc aacgacaggt tgggaggttc
     3841 cgattttggg cccttcctta tcccagttaa tgaaaaatcc atacaacaca actaaaaatg
     3901 tttgccaaaa cgccattttg ttctctcctt tcttcttata cttttatata agcaattact
     3961 gtgccaacta acattatcca ataaagcggg aaaatacagt aaatattaat taaatgagcg
     4021 tctaaatata ctttggacac cctttcattt ttttagacgc tagtatgcta tttagaaaat
     4081 agtctgctca aaaattgtat agattaactc atactctcct ttgggtattt caatacagta
     4141 aatatctttg atttctgaaa aaaaatcttt tgatacattt aaaaaagtat caaatagttg
//
LOCUS       DACEPW010000192         4190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEPW010000192 DACEPW010000000
VERSION     DACEPW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.197"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..846
                     /gene="nusA"
                     /locus_tag="IW819_002410"
     CDS             <1..846
                     /gene="nusA"
                     /locus_tag="IW819_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1245497.1"
                     /translation="KQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNL
                     GKIEAVLSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEV
                     PEVYDGIVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENM
                     DIVEWNEDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLA
                     AKLTNHKIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVE
                     EKPEA"
     gene            868..1161
                     /locus_tag="IW819_002411"
     CDS             868..1161
                     /locus_tag="IW819_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1245498.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            1158..1469
                     /locus_tag="IW819_002412"
     CDS             1158..1469
                     /locus_tag="IW819_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1245499.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            1482..3788
                     /gene="infB"
                     /locus_tag="IW819_002413"
     CDS             1482..3788
                     /gene="infB"
                     /locus_tag="IW819_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1245500.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            3815..4162
                     /gene="rbfA"
                     /locus_tag="IW819_002414"
     CDS             3815..4162
                     /gene="rbfA"
                     /locus_tag="IW819_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1245501.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         1538 a          800 c          916 g          936 t
ORIGIN      
        1 aaacaagtta ttttacagcg tgtacgtgaa gcagaaagaa cgattattta taacgaattc
       61 agtgcttatg aaaaagacat catgcaagga atcgtcgaac gccaggataa acgttatatt
      121 tatgtcaatc ttggtaaaat tgaggcagtt ctttctaaac aagatcaaat gccaaatgaa
      181 ttttatcaac cacatgatcg aatcaaagtt tatgtttccc gagtagaaaa tacgtctaaa
      241 ggtccgcaag tatttgttag ccgcagccat ccagacctat tgcgccgctt atttgagcaa
      301 gaagtaccag aagtctatga tggaatcgtt gaaattgtca gcatcgcccg tgaagctggc
      361 gatcgttcaa aagtggcagt tcgctcaaat gatccaaata tcgatgcagt cggtacatgt
      421 gtcgggccaa agggacaacg agttcaagca atcgtcaatg aattaaaagg cgaaaacatg
      481 gatatcgtcg aatggaatga agatccagct gtattcattg caaatgcatt gaatcctgca
      541 caagtcatgg atgtgatctt cgatgagaca aatccaaagg cctgcacagt tgtcgttcca
      601 gactaccaat tatcgctagc aatcggtaaa cgcggacaaa acgcacgttt agctgctaaa
      661 ttgactaacc ataaaatcga tatcaaatca gaatctgaca tgacggaatt ttatgaaaat
      721 caagaacaac aaaaagaaac agaagaactg catgatgaag caatcgttca gtcagattta
      781 acagacgatg aatatgaaac aattgcattc aacaatgaaa cggtcgaaga aaaaccagaa
      841 gcttaacttt agtaaggagg caaagagatg aaacaaagga aaattccttt acgcaaatcc
      901 gtggtttcag gtgaaatgaa acctaaaaaa gagatggtcc gaatcacccg ttcaaaagaa
      961 ggggaagtat cgatcgatcc aactggaaaa atgcctggac gaggagcgta tgtctcactt
     1021 gagccggaag aagtacaaca agcttgggat aaaaaaattc tcgatcgagt actcgaaaca
     1081 acattaacag atgagtttta tcaagaactt cttgactatg tcactcacca aaaagcacgt
     1141 aaagagttat ttggaaaatg aatacagtta atcggcaaaa agccatgaat ctcatcggtc
     1201 tagcgatgcg cgcgggaaaa ctaatcacgg gtgaagaact gaccattgct gaaatccgca
     1261 aacaaaaagc aaaattggtg tttgtggcaa ccgatgcaag cgagaataca agaaaaaaga
     1321 taaaagataa gagttcatat tatgaagttc cttgctttga gctattttct gaagcagaaa
     1381 tcacgcagat gatcggaaaa cctcgcaagg tattcggaat aatggatgcc ggatttgcga
     1441 agaagaccaa ggagctaatt gaaggttagg aaggtgattg catgggcaac aaaagaatct
     1501 atgaattagc aaaagaactc aaaaaatcta gtaaagacgt cgtggaaaaa gcacaacaac
     1561 ttggcatcga tgtgaaaaac catatgggtg cgattactag cagtgatgag aagaaattgc
     1621 aacaagcgtt caaaaaccca agcacgaaac aacaagctgc acaacaagca agcagaaaac
     1681 cagccaatca acagccgaac gatcaacgta aaaaagaaac aacgaacaat caacaaaaaa
     1741 ataaaaataa ccgcagttat caagatcgcg gtcaagggag cggtcaagtg aatcaaggaa
     1801 aaaaaccaac aacaaaccaa aataatacgg gaaataccag cagccaaaat cgtcaaggaa
     1861 cgagccaagg cggcaatcaa aaccgtacta acaataacaa caaaccaaac aataacaatc
     1921 gcggcaaatt taataataat aaccgtaatc gttataataa aaaaggtaaa aaaggcaaac
     1981 agcaacaatc aactaaacca gctgtaccac cacgcaaatt ccgtgaattg ccagatgtat
     2041 tagagtatac tgaaggtatg aacgtagcag atattgcgaa aaaaattcat cgtgagccag
     2101 cagaaatcat taaaaaatta tttatgcttg gtgtcatggt caatcaaaac caagcactgg
     2161 ataaagatac gattgaactg ttagcgacgg actatggcat ggaaccacaa gaaaaagtcc
     2221 aagttgacat tgctgatatc gataaattct tcgaggcaga agaagtcgat ccagataaat
     2281 tagtctcacg tccgccagtc gtaacgatta tgggacacgt agaccatggg aaaacaactt
     2341 tattggatac attacgccat tcacgtgtaa caagcggcga agccggcgga atcacacagc
     2401 atatcggtgc ctaccagatc gatattgatg gtaaaccaat cactttcttg gatacaccag
     2461 gacatgcggc ctttacaagt atgcgtgcac gtggagcaag tatcacagat atcacgatct
     2521 tagttgttgc tgcagatgat ggcgtcatgc cacaaacagt tgaagcaatc aaccatgcca
     2581 aagctgcagg cgttccaatc atcgtcgctg taaataaaat tgataaacca ggtgcaaacc
     2641 ctcaacatgt tatgcaagaa ttaagtgagt atgagttaat tcctgaagca tggggtggag
     2701 aaacgatctt tgttgagatc tcagcgaaat tcggccaaaa catcgaagaa ttgctggaaa
     2761 tgatcctgtt agtcgcagaa gtagaagatt taaaagcaga tccaacacaa cgcgcaatcg
     2821 gaacagtgat cgaagcacgt cttgataaag gtaaaggtcc tgtgtcaacc ttacttgtcc
     2881 aagaaggaac acttcatgtt ggtgacccaa tcgttgtcgg taatacttac ggacgtgtcc
     2941 gtgtaatgac aaacgatctt ggccgtcgcg ataaagaagc aggacctgca acgccagtag
     3001 aaatcactgg cttgaatgat gtacctcaag caggtgaccg tttcgtagtc ttcgaagacg
     3061 aaaagacagc acgtgcagcg ggtgaagaac gcgcgaaacg tgcattatta gaaaaccgtg
     3121 ctgccacaag ccgtgtgact ttggataact tatttgaaag cctgaaagaa ggcgaattga
     3181 aagaagttaa cgtaatcatc aaagcagatg tacaaggttc agcagaagca ctggctgcat
     3241 cattgaagaa gattgatgtg gaaggtgtcc gtgtaaaaat cgtccattca gctgtcggag
     3301 caatcaatga aagtgatgta accttagcag cagccagcaa cgctattatc atcggtttca
     3361 acgttcgtcc tacaccgcag gcaaaagtgc aagctgatac tgaagaagtc gatattcgtc
     3421 ttcaccgtat catctataaa gccatcgaag aaatcgaaac tgcgatgaaa gggatgctag
     3481 accctgaatt tgaagaaaaa atcaccggtc aaatgatcgt tcgtgaaacc ttcaaagttt
     3541 ctaaagttgg tacgattgca ggtgcctatg taacagaagg atatattcgt agagatagcg
     3601 gtgtccgtgt aatccgtgac ggaatcgtaa tctatgaagg ccaacttgca agtctgaaac
     3661 gattcaaaga tgacgtgaaa gaagtcaaaa tgggttatga atgtggtgca atgattgaaa
     3721 aattcaatga catcaaagta gatgacgtaa ttgaaggatt tatcatggaa gaagtcaaaa
     3781 atgactaaaa actgataaaa ggaggccaat cattatggct aactaccgtg accgccgagt
     3841 cggtcaagaa atcttaaaag aagtcaatga tattttgcgc aaaaaggtcc gtgaccctcg
     3901 tgtggagaac gtaacgatca cagatgtgaa agttacgggg gacttacagc aagcaacgat
     3961 ctattacagc attttatctg acttagcatc agataagaaa acagcacaag atggattgaa
     4021 taaagcatca ggattgattc gtcgagagtt agggcacaat ctaagtattt ataagacacc
     4081 tgaattgttc tttgaacttg atgaatcagt tgtctatggc aaccatatcg atgaattgct
     4141 tcgcgacttg aacaaagact aatcaaaaac tcccgcaaat cctgtgaaat
//
LOCUS       DACEPW010000193         4166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEPW010000193 DACEPW010000000
VERSION     DACEPW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.198"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(200..2365)
                     /locus_tag="IW819_002415"
     CDS             complement(200..2365)
                     /locus_tag="IW819_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1245502.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNRQADS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLEVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2625..>4166
                     /locus_tag="IW819_002416"
     CDS             2625..>4166
                     /locus_tag="IW819_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1245503.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENY"
BASE COUNT         1272 a          923 c          751 g         1220 t
ORIGIN      
        1 aacgaaaaat aggaggagct atttttgttt ggctgttctt attacttgat gcaggacagc
       61 tttttcacaa cctctcgata aatggtgttc aatcagctga ccttaggtat tagttcaaaa
      121 aacggcaaaa tacgaggagc tgttttttcg ttttgtttct ctaatactca ggtcaggaca
      181 atgatttcac aacctctcct tacattttaa tggagccgat cactttacca gctgtcactg
      241 tacctaattg cttgatcact acttcttcta ctttatcgtt tgtaaatacg atcatgatcg
      301 ttggatcttt ttcttcattt tggatcacct caagatccat ctcagctaat ttcgttcctg
      361 ttcctacttc ttggccttct ttgacagcaa tctcaaaagc tggctgattc atttggactg
      421 tatcgatacc catatggatc aaaacttcga taccatcttt cgtctccagc ccaattgcat
      481 gtttgcttgg gaaaatactt gtcacttttc cagcgatcgg cgcaacaact tctcttgatg
      541 ttggaataac agcaaagccg tcacccatca ttttttgtga gaataccgga tcttttactt
      601 cagataaagg aatcacttct ccagaagctg cggaaactag ttcttgttct atacctaagt
      661 attgcttact atctgcttgt ctatttcctg tagaaaccat ttctggctct ggaatctctg
      721 ctcctgattg cagcagatct tcgatatctg atttcaagac atctgctttt ggtccataaa
      781 ctgcttggac tccattgtct ttgacgatca atcccatcgc ccctgctcgt ttccaggctt
      841 cttcagtccc tactttttct cgatcattta cacttacgcg taaccgagtc atacaagcat
      901 cgacatcttt gatattttct tttcctccca gcaaatgaat gatctggacc acttgctgat
      961 ctgctgttcc tgttccgctg ttagatacaa cggtttcttc actgtcattg tcgtaatttc
     1021 cgttacgtcc cggtgtagca aagttgaatt ttttgatcaa gaaatttgct aagaaatacg
     1081 taacaacacc aaaagcgatc acacacaaca cgaagttgat aagatctcca cctaaacccg
     1141 ctttgatagc taacggtgtc cgagtcagca attcgatatt cccaaatgaa tggacccgca
     1201 atggcaagat atctgccatc gcaaatgcag ccccttggat cactgaataa atgacatata
     1261 atggcacagc agcaaacata aacatgaact ccaatggttc tgttacacct gttagaaaga
     1321 cagccaacgc tgctgaaaaa tacattgatt tatatcttgc tttcttatca ggatcgacat
     1381 tacgatacat cgcaaaagcc attcccatca agatacctga agaaccaatc atttgtccta
     1441 ctttgaaacg agccggcgtc cagttatgca acacaaactc atattgcgac atatcccctg
     1501 cacctttaag attcactaaa tcagtggccc aagccaacca taacggatct tgcccaaata
     1561 cttgtgtccc cgcctgtgcg cctgataaaa tctcatacga tccgccaagc tgtgtatagt
     1621 tgataggaat cgtcagcata tgatgcaatc caaatggcaa tagtaaacgt tctaatgttc
     1681 caaataaaaa cggtgctaga atcggtgcgc tttcttgtga ttctgcgatc cataaaccaa
     1741 aattattgat tccagcctgt acgtttggcc acaccacagc aagaatcaat gccacgattg
     1801 ttgaccataa gataacaaca aacggaacaa agcgctttcc attgaaaaat gacaaagcat
     1861 ccggcaattt tctataatta tagtacttat tataagccat tgccccgaca aatccagcga
     1921 tgatccctac gaatactccc atattcaaag ctggtgcttc caatacactt gtaaaaaatc
     1981 ctttaaccat gatcttcgta ccgaacagag tgtgggtaaa tgcatcttca ttcgcaagca
     2041 tgtcacttgt gacaccaaag atagccccag tgatccgatt gatcaaaata aatgagatcc
     2101 cagctgcaaa tgctccgcct gctctttctt ttgcccaact tcctcctatt gctaaagcaa
     2161 ataaaatatg aagattacca ataattgccc aaccaatatt ttcaattact ccgcccgttg
     2221 tgacaagcgg cgtccaatca ggattgatca agggaattga tttccctata ctgatcatca
     2281 acccagctgc cggcataact gctaccacaa ccattaaagc ctttccaaat ttttgccaaa
     2341 actcaaaact aaatatcttt ttcattccgt ttagctcctt tacgcaatcg gtttcgttaa
     2401 acgtattata gcgtgcgttt tcttttttgt aaatgcttta ttttttattt ttctgtctat
     2461 accattctag aaaacctata atgcgtgtgt tcccttctat aattttctcc taaaaattta
     2521 tttttttatg cttttttcta tgaaatattt tcttttgtct attgacttgt gcaatcggtt
     2581 gcgtttatta tgaacgtata aaacagctca ttaggaggag aaaaatgaaa cagatcaaac
     2641 gactatttgc tattgatcca tggaaaatca gcacgacaca attagataaa aaaaatcttc
     2701 gactgcaaga atctttaaca agtattggaa acggttatat ggggatgcgc gggaactttg
     2761 aagaaactta ctcaggggac caccatcaag gtacttattt ggcgggtgtc tggtatcccg
     2821 ataagacacg tgtcggctgg tggaaaaatg gttatccaga atactttgga aaagtgatca
     2881 acgccttgaa ctttatcgcg atagatattt cgatcaacga tcagctgatc gacttggctt
     2941 ctttggaacc agaagacttt tactgggaat tagatatgaa aaatggtgta ttatctcgta
     3001 cattcacaat cactacttct actaataaag tacgcatttc cttcgaacgt tttctgagca
     3061 ttgtcaaaaa agaatcagct tatatctatt taactataga aatgcttgaa ggtagcggaa
     3121 cagtcaaact cgtatcaaaa ttggatggaa atgttcaaaa cgaagacagc aactatgatg
     3181 aacatttttg gaatgagatt gatcgtggaa ctatagaaga aatcggctat ttgacgacta
     3241 aaactattcc aaacaacttt gggattgaac aatttactgt tactgcagca atgcgccatt
     3301 tcgttaatgg aaaatcagtc atccctgact atcacgaaga tactttagca ttacatggtt
     3361 gcttgtctgc agaattatct caaggtgaga catggtcagt ggaaaaagaa attatcgttc
     3421 ttactagtag agatattcca gaagcgcagc aagcagaaaa agctgtctca gaattaacga
     3481 accagacaaa caattatatc caagcaaaag aagagcaaac agcggcttgg gcagaacgat
     3541 ggaagcttgc cgatgtggtc atcgaaggag acgaagaagc acaacaaggt attcgattca
     3601 atttgttcca actattctct acttactacg gcgaagatga acgattgaat atcggcccca
     3661 aaggattcac aggagaaaaa tacggcggag ccacttactg ggatacagaa gcgtatgctg
     3721 tccctcttta tcttgcttta gccaaacctg aagtaactaa gaatctgctg aaatatcggc
     3781 ataatcaact gccgcaagca atccacaacg ctcagcaaca aggattaaaa ggcgcgcttt
     3841 atcctatggt caccttcact ggtgtcgaat gccataacga gtgggaaatc acattcgagg
     3901 agattcaccg aaatggcgcg attgcctatg caatctacaa ttatacgaat tacaccggcg
     3961 atacggaata tttgaaaaat gaaggactgg aagttttagc agaaatagca cgcttctggg
     4021 cagaccgtgt ccatttttca aaacgagcaa ataaatacat gatccacggt gtgaccggcc
     4081 caaacgaata tgagaacaat atcaataaca attggtatac aaatacgatc gcagcttggg
     4141 tcttgcgtta cacgcgagaa aattat
//
LOCUS       DACEPW010000194         4154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEPW010000194 DACEPW010000000
VERSION     DACEPW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.199"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(41..403)
                     /locus_tag="IW819_002417"
     CDS             complement(41..403)
                     /locus_tag="IW819_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245504.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            749..2188
                     /locus_tag="IW819_002418"
     CDS             749..2188
                     /locus_tag="IW819_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1245505.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(2195..>4154)
                     /locus_tag="IW819_002419"
     CDS             complement(2195..>4154)
                     /locus_tag="IW819_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1245506.1"
                     /translation="SGSAVGVVVETGNTTEIGKINQALQSVEQQTTPLIKKINRLNKQ
                     IFQGILCLSLFLVIFTTFRYGMDWHILLSATIALVVSMVPEGLPAVLTMILSVGVHEM
                     AKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNEMTVVDVAAKEEACVLSIMKNCQEL
                     KTKEEQKTENLSGNPTEVALIRHTETANLPLKKIEAKIPFSSEYKYMATMHAEEKGAV
                     IYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARKGQRVLGFAYKKVDSKQELTHETLT
                     QLTFAGIAGLIDPPKESAVKAVKECQQAGISVKMITGDHKDTAKAIAEQIGLKHTAKV
                     LEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLRIVKALQKNGEIVGMTGDGVNDAPA
                     LKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTIAKAVKEGRRILDNLQKTINFFLPT
                     ALAQGLILIWALMLNRPLPLSPVQILWVNMVTTITLSYALGFESPDPEIMKRPPRDPK
                     QGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQSLQQTILLQNIVLAQAVYMINCREL
                     SKPSLNQGLLQNKALFLSLGILALLQGMVIFLPVGQQLIGTVSLTMTQQLLIGANVIL
                     LFLVVEIEKGIMNKLFRKKEKTAREYSR"
BASE COUNT         1203 a          863 c          691 g         1397 t
ORIGIN      
        1 aacatttctg tcacaacctc ttctctattg tcctagacat tcattgcgtt gatttaaaac
       61 ttccatcaat caaatctgct agttcatcta gcttttctat tttacgtaga tcaatagaga
      121 tccaatgttc tttattcatg tgataggctg gaaaataccc ctcttttcct cgcaaaattc
      181 cgccaagttc aggatcgacc ttcaaatcaa gaatattgat gatcctatgg tcacttgcat
      241 catcatagat tttttcttct ggaacagaca tcaggacccc aaaccatttt ttgctttttt
      301 gatgtctgaa tacgcaataa tcaggaaatt ttttaaaaat atattctgga gacaaattgt
      361 atgtttcatt gatgtgatcg attagttctt ttttcgttag catctgcttc catctctttc
      421 ttattgattc gctttattca aagataccac gtaagaatag aaaaatgaat acaagtagtt
      481 gctaatcaaa tacttcgata tttttctttt ttatcaactc ttaaatgttc aatccgcctg
      541 tgttggtctt gctaccatct actattaaaa tgtttattat tgtttgccca cctatttttt
      601 gattttagtg gtatcatttt tcaagttcat gatgaaattc ttatcatcag taaatgctcc
      661 agaaaaataa acaatgattg aaaaatcagt tgaaacttga ccaccgttta ttttctccgt
      721 attcttagaa tagaagggag tctgattgat gtattcacta atgaaaaaaa tcattaccga
      781 aaaagatatt cgacgtcatg tttctttagt agaacaattg ctgaatcata caaagataac
      841 cgtaaatgaa ttagctgaaa tcatagggac aaccgaacga acgatttttt ctgaccttca
      901 atctattcgc tctcagctgc ccgaaggctg ggacatcatc agcgatcaag caggaatcag
      961 tttgcaaaac cagcaaaatt tattaacaaa tgacctttgg gagatttttt tcaaacaatc
     1021 agtcagtgtc gaactgctga aaaatctgct ttttacaaaa aaagttgctg tccctgattt
     1081 tctagccgac catgggcttt cttatgggac attgaaaaga catgtgacta aaatcaatca
     1141 gcgtttagca agttacgact tgcagatcga tttgacgaaa tatacagctt gtattcttgg
     1201 aaaagagcgt gcgattcgta ctttttatca tcgattgctg attcctttca ctcataacaa
     1261 ctattttttc gaggattatt cgatccatga atcccattat tttcaatttt taagaaacct
     1321 cagtcaaact gaactagcgg ttgaaacaga agagattttc ggaacatgtt ggttctttat
     1381 caatacgatc cgtataaaag cgaattgccg attagattct tctatccata ccaacagcac
     1441 actctcctcc ctgtatgact ctgcattgaa aaaattgtat ttaacggaag gtatttatct
     1501 taaagacact gagctttctt ttgcttcttt ctgctttctg gaaagttgga attacaacaa
     1561 taactatgga caagaaaccg ctcgctgcct gcatcattct ccatttttag aggtcctcga
     1621 aacgtttgta gaggaactag cttcagagct gtctcttgat caactaaaaa aaacatctct
     1681 gactgataac ttggctctcc tcatattaaa atatcatgaa tcgccgatat tgattgaagc
     1741 attggaccgt caataccatc gttttttaga aacatatgat cagcaatatg cacacttgta
     1801 tcaacagaaa gagaatttac tgaacgatct aaaaaaagaa atagaaatca atgaaccaaa
     1861 ttactttctc caactcttgt ctctactgat ccaacaaacg attttttcta tcaaaccgaa
     1921 tttgttcaat gtctatttta ttttccaagg agaaccttca tggaaggcgt tcttgcagca
     1981 ggaattaaaa gattacttag gaaaacgtgt ctgcttctta cctatcgaac taaccgatct
     2041 ttccactatt tcttttgaaa aaacagatat cttgatttcc aatttccctc tagattctct
     2101 agatatccct attgtataca tttcttctat tccaacgaaa aatgagctca atcgtttgac
     2161 tgaactcact ttcaaaactt ttttatagta acggctagcg actgtattct ctagctgttt
     2221 tttctttttt gcgaaacagt ttgttcatga tccctttttc tatttctaca actaagaata
     2281 gaagaatcac atttgctcca atcaataact gttgtgtcat cgttaaactt actgtaccga
     2341 tcagctgctg gccgactggt agaaaaataa ccatcccttg caataaagca aggataccta
     2401 atgaaagaaa aagcgctttg ttttgcaaaa gaccttggtt caatgacggc ttggacaact
     2461 ctctgcaatt gatcatgtaa acagcttgag ctaagacgat attttgcaga agaatggttt
     2521 gctgtaagct ttgcccttca aactgcatag ccaataaata ggcaggaacc atgatcagta
     2581 gggagacata gatgatccga aacaaatgat aaccagatag gattccttgc tttggatctc
     2641 ttggtgggcg cttcatgatt tctggatctg gcgactcgaa ccctagtgca taagataaag
     2701 tgattgtcgt taccatattg acccataaaa tctgtactgg ggacaaaggt agcgggcgat
     2761 tcagcataag tgcccaaatc aagatcaatc cttgagctaa tgcagttggc aagaagaaat
     2821 tgatggtttt ttgcaaatta tccaaaatcc gccggccctc ttttacggct tttgcaatcg
     2881 tatggaaatt atcatctcct aaaaccatat cagctgcctg tttgcttact tcactacctt
     2941 tgattcccat agcaattcct acatcggatc ttttcaaagc aggtgcatca tttacgccgt
     3001 ctccggtcat tccgacgatt tctccatttt tctgcaaagc tttaacaatt cgcagtttat
     3061 gttctggtgt cgttcgagca aagacatcga ctattggcac ttgctgaatc aactcttcat
     3121 cagacataag atccaaatcg atcccttcta aaactttggc agtatgtttt aagccaattt
     3181 gttccgcaat cgcttttgct gtgtctttgt gatcccctgt aatcattttc actgagatcc
     3241 ctgcttgttg gcattccttt acagctttga ctgcactttc ttttggcgga tcgatcaatc
     3301 cggcgatccc tgcaaaagtt aattgggtta aagtctcatg cgtgagttcc tgtttcgaat
     3361 ctactttttt ataagcaaat cctaaaacac gctgaccttt tctcgcaaat tctgcagctg
     3421 tctgactcca tgcttcttgt tctgcagaag aaagagttga tttagcaaat aatacttctg
     3481 gtgcaccttt gacgtaaatc accgctcctt tttcctccgc atgcatcgtt gccatatatt
     3541 tgtattcaga actgaacgga atctttgctt ctattttttt caaaggtaga ttggctgttt
     3601 ctgtgtgtcg aatcagcgca acttctgtcg gattgccact taaattttct gttttctgtt
     3661 cttcttttgt ttttaattcc tgacagtttt tcataatgga caacacgcag gcttcttctt
     3721 ttgcagctac atccacaact gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac
     3781 agatcactgt catagaacct aatgtttcta ccgaaggaag tcctttgatg atcgcctttt
     3841 cttttgccat ttcatgtact ccaacggata gaatcatggt caaaacagct ggcaatcctt
     3901 cgggtaccat tgacaccacc aaagcaatcg tagccgaaag aaggatatgc caatccatac
     3961 catatcgaaa tgtcgtaaaa ataactaaga ataggctcaa acaaagaatg ccttgaaaaa
     4021 tttgtttgtt taatcggttg atttttttaa tcagaggcgt cgtttgttgt tcgaccgatt
     4081 gcaatgcttg attgatttta ccgatttctg tcgtgtttcc tgtttctaca acgacaccga
     4141 ctgcagaacc agat
//
LOCUS       DACEPW010000195         4151 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEPW010000195 DACEPW010000000
VERSION     DACEPW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.200"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            102..1046
                     /gene="mvk"
                     /locus_tag="IW819_002420"
     CDS             102..1046
                     /gene="mvk"
                     /locus_tag="IW819_002420"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1245507.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1039..2016
                     /gene="mvaD"
                     /locus_tag="IW819_002421"
     CDS             1039..2016
                     /gene="mvaD"
                     /locus_tag="IW819_002421"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1245508.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2029..3114
                     /locus_tag="IW819_002422"
     CDS             2029..3114
                     /locus_tag="IW819_002422"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1245509.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3111..>4151
                     /locus_tag="IW819_002423"
     CDS             3111..>4151
                     /locus_tag="IW819_002423"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1245510.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRT"
BASE COUNT         1287 a          863 c          906 g         1095 t
ORIGIN      
        1 gagaccctta gtggacaaaa aaattcgtga agcagcgcaa ctcgttttgg gcgctttatt
       61 ttttgcttaa aaatcacttt tacgtgagaa ggagttagat tatggcaaac tatggccaag
      121 gagaatcaag cggaaagatc atattgatgg gcgagcacgc ggttgtttat ggagaaccag
      181 cgattgcctt tcctttctat gcaacaaaag tcaccgcatt ccttgaagag ctggatgcaa
      241 tggacgatca actggtttct tcctactatt caggaaattt agccgaagct cctcatgcat
      301 taaaaaatat caaaaaatta ttcattcact taaaaaaaca gcatgacatc caaaaaaact
      361 tgcaactgac cattgaaagc acgattcctg ctgaacgtgg aatgggatca agcgctgcag
      421 tcgccacagc agtcactcgt gctttttatg attacttagc atttcctttg tctcgtgaaa
      481 tactattaga aaatgtccag ctttcggaaa aaatcgccca cggtaatcct agtggaatcg
      541 atgcagccgc tactagcagc ttgcagccga tttattttac aaaagggcat cctttcgact
      601 acttttcttt gaacatcgat gcttttttga ttgtcgctga tacaggaatc aaaggacaaa
      661 caagagaagc cgtcaaagat gtcgctcacc tctttgaaac ccagcctcat gaaactggac
      721 aaatgatcca aaaattagga tacttgacga agcaagcaaa acaagcaatc attgaaaatt
      781 caccagaaac gttggcacag acaatggatg aatcacaatc acttctggaa aagctgacaa
      841 tcagcaatga ttttcttaat ctattgatcc aaacagcaaa agataccggg gcattgggtg
      901 ccaaattaac tggcggtgga cgcggtggct gtatgattgc attagcacaa acaaaaacaa
      961 aagcccaaga aatcagtcaa gcacttgaag atgcgggtgc tgctgaaact tggatacaag
     1021 gattaggagt acatacctat gtttaaaggc aaagcacgcg catataccaa cattgctcta
     1081 atcaaatatt ggggtaagaa aaatgaagaa ctcatccttc caatgaacaa cagcctttcg
     1141 ttaacattgg atgcatttta tacagagaca gaagtcatct tttctgatag ctatatggta
     1201 gatgaatttt atctagatgg caccttgcaa gacgaaaaag caacaaaaaa agtcagtcag
     1261 tttcttgacc tttttcgtaa agaagccggc ctctcactaa aagcttcagt aatcagtcag
     1321 aatttcgttc ctaccgcagc tggattagcc tcctctgcca gcgggctagc tgctttagca
     1381 ggcgcttgca atactgctct taagcttgga ttagatgatc tctctctttc aagatttgct
     1441 cgacgcgggt ctggttcagc ttgccgaagt attttcggtg gtttcgtcga atgggaaaaa
     1501 ggccatgacg acttaagttc ttacgctaag ccagtccctt ccgattcttt cgaagacgat
     1561 ttagcaatgg ttttcgtttt gatcaacgac cagaaaaaag aagtgtccag cagaaatggg
     1621 atgcgtcgga cagtcgaaac atccagtttt tatcaaggct ggttagattc cgttgaaggg
     1681 gatctatatc aattgaaaca agcaatcaaa acaaaagatt tccaacttct cggagaaacg
     1741 atggaaagaa acggactaaa aatgcatgga acgactttag ctgcccagcc tccattcact
     1801 tactggtctc ctgacagctt aaaagcgatg gatgcggtgc gacaattgag aaagcaaggc
     1861 attccatgct actttacgat ggatgctggc cctaatgtca aagttttagt ggaaaacagc
     1921 catttatctg aggtacaaga aacatttacc aaattattta gcaaagaaca agtaattact
     1981 gcacatgctg gtccaggaat tgcgattatt gaataaaggg agatttttat gattgaagta
     2041 tctgcaccag gcaaactcta tattgccgga gaatatgcag ttgttgaaac aggccatcca
     2101 gcagttatcg ctgcagtcga tcaattcgtg acagtcactg tagaatctgc acgaaaagtc
     2161 ggaagtatcc aatctgcaca atatagtggg atgcctgtac gttggacaag acgcaacgga
     2221 gaattagttt tagatattcg ggaaaatcct tttcattata tccttgctgc tattcgcttg
     2281 actgaaaagt atgcacaaga aaaaaacatt cttttatcct tttatgatct gaaagtaaca
     2341 agtgagttag acagttcaaa tggccggaaa tatggattgg gttctagcgg tgccgtgacc
     2401 gttgcaacgg tcaaagcctt gaatgttttt tacgcgttga atttatctca gttggagatt
     2461 ttcaagattg cagcactagc caatttagca gttcaagata atggttcctg cggcgacatc
     2521 gctgctagct gttatggtgg ctggatcgct ttctcaacct tcgaccatcc ttggctccaa
     2581 gaacaagaaa ctcagcattc tatcagtgag ttacttgccc tggattggcc aggtctatcc
     2641 attgagccat tgattgctcc tgaagattta cgtttattga ttggttggac gggtagccct
     2701 gcctctactt ctgatttggt cgatcaagtt caccgttcga gagaagataa aatggtggct
     2761 tatcagcttt tcttaaaaaa tagtacagaa tgtgtcaacg aaatgatcaa agggtttaaa
     2821 gaaaataatg taacgttgat ccaacagatg attcgaaaaa accgacaatt actgcatgat
     2881 ttatctgcaa tcactggggt cgtcatcgaa acgcctgctt tgaacaaatt gtgtaattta
     2941 gctgaacagt atgaaggagc cgcaaaatct tctggtgcag gtgggggcga ttgcggaatc
     3001 gtaattgttg accagaaatc tggcattctt cctttaatga gtgcatggga aaaagcagaa
     3061 atcactccac tgccgttaca tgtctatagc gatcaaagaa aggaaaaccg atgaatcgaa
     3121 aagacgaaca tgtttcacta gctaaagcct ttcatgataa acaaaaaaac gaatttgact
     3181 tcgtacggat catccataac cctttgccgc aaatagcagt ttctgatgtc gatctaagta
     3241 cacaggcagt tgggtttaca ctaagcagcc cattttatat caatgcgatg actggcggaa
     3301 gcgaaaaaac aaaaaaaatc aaccaagatc ttgcaatcgt cgcaagagaa gcagatttga
     3361 tgattgccac tggttcagtc agcgctgcct tgaaagtccc ttctttagcc gatacttata
     3421 cgattatgcg acaagaatat cctcacggga aaatcattgc caacatcggt gcagggactt
     3481 ctgtcgaaag agccaaagaa gctatccgat tgtttcacgc agatgcctta cagattcatt
     3541 tgaatgcacc tcaagagttg gttatgcctg agggagaccg tgattttacc aattggaaag
     3601 tacttattca agaaactcaa acagccattg acgtccccct catcgtaaaa gaagtcggtt
     3661 ttggcatgac aagagaaacg ctcaatgatc tagctgcttt aggagttcat acagttgata
     3721 tcagcggtcg aagcggaaca agcttcaccc agatcgaaaa cgcccgtcgc tccaagcggg
     3781 aactgagtta tttagctgac tgggggcaat cgacagtctc ttcattgctt gaagcaaatg
     3841 aagcagacac ttctatggaa atcctagcct ccggaggtat ccgcaatgcc tatgacatat
     3901 ttaaagccct ttgtctcggc gcaaatgccg tgggtacttc aggaaccgta ctgacgcatc
     3961 tgatgaatca cggcgtagaa gaaactatta tactgatgaa gcaatggcaa gaagagcttc
     4021 gtctgcttta tactatggtc ggcgcaacga ataccgcggc cctacaccaa caatctctga
     4081 tcttttccgg accagtgaag gattggtgcg aagcaagagg gatcgactta gtgaagtatg
     4141 gaagacgcac a
//
LOCUS       DACEPW010000196         4109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEPW010000196 DACEPW010000000
VERSION     DACEPW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.201"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            45..779
                     /locus_tag="IW819_002424"
     CDS             45..779
                     /locus_tag="IW819_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1245511.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            818..1960
                     /locus_tag="IW819_002425"
     CDS             818..1960
                     /locus_tag="IW819_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1245512.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(2055..3446)
                     /gene="sufB"
                     /locus_tag="IW819_002426"
     CDS             complement(2055..3446)
                     /gene="sufB"
                     /locus_tag="IW819_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1245513.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(3451..3921)
                     /locus_tag="IW819_002427"
     CDS             complement(3451..3921)
                     /locus_tag="IW819_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1245514.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(3908..>4109)
                     /locus_tag="IW819_002428"
     CDS             complement(3908..>4109)
                     /locus_tag="IW819_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1245515.1"
                     /translation="HDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTT
                     QDADRLVEAIMATKEFFKHGTI"
BASE COUNT         1286 a          769 c          808 g         1246 t
ORIGIN      
        1 cacccctttt ttgaggtata ctaattttag gaggtgtggt caatatgata aaacgtccac
       61 caataaatta tttagaacga aaaaagatac tcggaacaaa aataaaagct ataagaaaga
      121 gtaaaaaatt aacacaacca gcatttggtc ttatgattaa caacggtcaa ttaattgaca
      181 aaaaaactat ctacgaatgg gagaagggaa cctatttacc aataccagag cgattatccc
      241 gaattgctga tttgggaaat atgagtatag aggagttagt ttgtggcaat gtagaagaat
      301 atattttggg aattattctt tatagagata gcattgtact agatggtata acatttccag
      361 ataaaaacct gttccagcat cttcgtcaac agttcccacc agtacactct aatttagata
      421 catggctgga tagatattcc aaattagaac cagagatgca agaatttatt gctaacaaga
      481 cttgtaataa agttaaaaat gaaaaaatta gtcttttcaa tatcttaaaa atagaagaat
      541 tattcatcaa tgctattgta gaagaatttg acaataacat tcttttcctc actagcagta
      601 tagaagaact actagaaaga atggtagatg aatggctacc tattcaattg aaagacatgt
      661 cttatcccga agaagctgtg agagaaatta ctgataatat aaacaagcta gaacaaacga
      721 tttcatcaat tgggaaaaaa tacactaaga aaaaaatgaa gggaggtgat accatctagt
      781 ggtaagtact ccttgtaaac taagaggaga tgatttaatg gcaaaaatat caaagtacaa
      841 aaagaaaaac ggtcaagtgg cgtggatgta taaaggacac attgccaccg atccacgaac
      901 tggtgaaaaa ataaatacta cgagaagagg ttttagtagc aaacaagaag ctcaaaaaga
      961 ttatgacgag tacagacatc aaatattata cggtgttaaa aagaaagcac ctgatatgac
     1021 atttgaagac ctgtataatg aatggataac tcatcagcgt accagtgtta aagcttcaac
     1081 aatcgcaatc tcagtaagat acgcaaataa tcaaatactt ccagcatttg gtaagcttaa
     1141 actatcgaat atctctgtcc catattgtca aaaagtggta gatgaatggc acagtaaata
     1201 tgaaagttac gattatatga gaaaacagac tgcacaaatt ttacgctacg gggttgcaat
     1261 gcaatatatt gacaataatc ctatggaaaa aacacttctt ccacgaaaaa aagagtatga
     1321 gaaaaatcgt aaattttact caaaagagga gcttaataat ttactggatg cttttaaaga
     1381 ttttggtaac atgaaacaat acgccttctt cagattattg gcatacacag gtatgagaaa
     1441 gagtgaagta ctcgctttac agtggaaaga catagacacg tttaataaag aactacatgt
     1501 caataaaact cttgctgtag atgaatttgg taaagttata attcaatctc cgaaaacaag
     1561 agcttctaga cgagttatct ctttagatac tgagactctt tctatcctca acaactggaa
     1621 attacagcaa aaagaagagt atttaaaact tggatacaat actagctcta aagagcaaca
     1681 tgtttttaca acagttaaaa acactctata cattccaaac actgttaatg attggttaag
     1741 atatattttg aaaaaatata acttgcctcg tattacacct catgggttta gacatacaca
     1801 tgctagttta ttattagaag caggagaatc tgtcaaggta gttcaacaac gattagggca
     1861 tgaaaatagt aaagtgacat tagacatcta tgcacatatt acaaataatg cacctaagaa
     1921 aacaggtcaa gactttgctg atatgatggc tcatcagtaa aagagctggt caaaatctgg
     1981 tcatttctag tatacaggca aaacaaaaaa gctagaaccc ttatatatca agggttctag
     2041 ctcctataac aggtttaccc aacactgcct tccatctcat aactgatcaa tcggttgagt
     2101 tcgactgcat attccattgg aagttctttg gtgaatggtt caacaaagcc catgacgatc
     2161 atttcagttg cttcagattc ggtcaggcct cggctcatca gatagtagag ttgttcttct
     2221 gagatttttg agactttggc ttcgtgttct aaagagactt ggctgttgtg gatctcattg
     2281 aatgggatcg tatcggattt cgaccattcg tccatgatga tcgtgtcaca ttcgatatga
     2341 gagatcgaac ccgcactgtt tttggcaaag gttacttgtc ctcgatagtt tacttctccg
     2401 ccgtcttttg aaattgattt agagacgata gagctagatg tatttggtgc attatggatc
     2461 atttttgcac ccgtatcttg gatttggttt gctcctgcaa aggcgatgga gagcatggtg
     2521 cctcgtgcac cttttccatc taagtaaaca cttgggtatt tcatcgttgt ttttgcacct
     2581 aagtttccgt cgatccattc aacggttgcg ccttcgtatg cttttgctcg tttggttact
     2641 aagttgtaca cattgtctga ccagttttgg atcgtggtgt aacggcaata cgcatctttt
     2701 cttgtaaaga tttctacgat ggcagcatgc agactattac ttgaataagt aggtgccgta
     2761 caaccttcga catagtggat gcttgctcct tcgtcaacaa tgatcagcgt acgttcgaat
     2821 tgccccgtgt tttccgcatt gatccggaag tacgtttgta atggaacatc gactttgacg
     2881 ccttttggca cgtaaataaa ggtgccacct gaccatacgg ctgaattcag cgctgctaat
     2941 ttgttgtcgg ttggcggtac aagttttgaa aagtactctt tgaataattc agggtattct
     3001 ttcaaagcag agtcagtgtc agtaaagacg attcctaact tttcgaattc atctttcata
     3061 ttatggtaca cgacttctga ttcatattgt gcggaagcac cggctagata agctcgttca
     3121 gcttctggta taccaatctt ttcaaatgta tctttgattt tttctggtac gtcttcccag
     3181 tcacgagcgg gtttgtcact tggtttttgg taatatttga tggcatcgaa atcgatgtct
     3241 gaaaggtcgg gcccccatgt ttgcattggc attttattga atgtttctag agatttcaga
     3301 cggaattcaa gcatccattc tggttcgcct ttgattcttg acatctcacg gatcacttct
     3361 tctgtcaagc cttctcctgt actgaaaacc ggttcgacat catcgtgaaa cccgaattta
     3421 tattcttcta attctggtac gcccatgttc tcactcctct tccttgtggt aatgatggaa
     3481 agctgcggta ccatcgtttt ccattgcttt tcctagtgct ttccaggcaa gtgtcgcaca
     3541 tttgatccga gcgggaaatt tagctacacc accaagcata gcagcatcgc ctaattggtc
     3601 gggatctttg acttcttttc cttggacaag ctgtagaaag tctacagtta aatgttctgc
     3661 ttcttctatt gattttccaa tgacggcatc tgtcatcatc gaggcacttg ctgtactgat
     3721 ggaacagccg ctgccgtcaa aggcgatatt tttgatgatt ccatctttta catcaacttg
     3781 caattcgatc acatctccac atgtggggtt gttcaattcg atcttgcttg tcgagtcagt
     3841 caagcttcca cgatgatgtg gatgggaaga atgatccaag atcacttgac ggtataaatt
     3901 gtctaattta gatagtccca tgtttgaaaa actcctttgt tgccatgatt gcttcaacta
     3961 aacggtcggc gtcttgcgtt gtgttgtaga aataaaagct ggcgcgagca gtcgctgata
     4021 cttcaaggta cttcaatagc ggttgcgcac aatgatgacc tgctctaacg gctacccctt
     4081 ccatgtctaa agctgtcgcg acatcatgg
//
LOCUS       DACEPW010000197         4097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEPW010000197 DACEPW010000000
VERSION     DACEPW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.202"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..556)
                     /locus_tag="IW819_002429"
     CDS             complement(26..556)
                     /locus_tag="IW819_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1245516.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(738..1394)
                     /locus_tag="IW819_002430"
     CDS             complement(738..1394)
                     /locus_tag="IW819_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1245517.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1521..1994)
                     /gene="dfrF"
                     /locus_tag="IW819_002431"
     CDS             complement(1521..1994)
                     /gene="dfrF"
                     /locus_tag="IW819_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1245518.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2413..2631)
                     /locus_tag="IW819_002432"
                     /pseudo
     CDS             complement(2413..2631)
                     /locus_tag="IW819_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2662..3876)
                     /locus_tag="IW819_002433"
     CDS             complement(2662..3876)
                     /locus_tag="IW819_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1245519.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1274 a          869 c          585 g         1369 t
ORIGIN      
        1 taacagcttt tctgtttctc tctagttatc tacgcatata aatcaaatcg gttattccgt
       61 tataagtctg tgttttacaa agccttaact ttatttccct gtctatgttg cccaaaagac
      121 gaaccccatt tcctaacaga gtaggaataa cagaaatgta atatttatca atcatatttt
      181 tgcttaccaa ctgctgaaca agatttgctc cgccacaaat ccaaatatcc tttcctgctt
      241 cttgttttag ccgttccaat aataaaacag gattttcact tgtaaaatga atttgttggg
      301 aagaattgca ctctttatgt gttaatacat aagttgtaaa tttattatat acccattctt
      361 gcggagaaag ttcagtaaca acctgatgag aagtattcca ccccattaag acagtatcta
      421 tatctttggt aaattctgta taagtgtcga tgttttcatt gtcatttcca tgaccattta
      481 gccaagctac cccaccatta ccgtccgcaa tatatccgtc aaggctcatg gcaataaata
      541 aaactacttt tctcattttt tctcttttat aaatacaaac aggaatttat ttctcttttt
      601 tactcataag ggaacaactg attgctacaa ataaacccat taaaatccat ggaaatgctg
      661 ctcttataaa atcactaacc atactttatt cctccttaaa ttccaatttc cttagttcta
      721 caaactgaaa tttttgccta tgaatgtatc ttgttcttta tacgcaaaca ggcttctgtg
      781 tgcataatcc aatgtcttga aaacggcatt tgaattagtc cacaaatatc tttattacac
      841 caataatcaa tcagccaaat tgctttttca ttgtcgttta ctacattcaa cgattctaag
      901 tatccttttc tttctttcgg tatttttctt ttcaattcat cataagataa ccgttcaagc
      961 attttctcag tgctttccca tacttcgaaa atatataaat ataattcttc cagattaagc
     1021 tgttttgaaa agtctgcaat ctgctgtttc atgagttcat ttcctgttgt aatgataggt
     1081 gaattgatac gctcttgata attgcctgca aaaaatactt gttcatcttc atttatcact
     1141 gtatgcacaa ctatatcttc aatgcggaaa atatgccaaa tagaataagc aatcgtctta
     1201 ctatgataac catcagcgtt tatgaagggg attgagttaa aatcttctct gcataattct
     1261 tctttgaaag atactaaggt ttgtattaac tgacttcgta aagtaagtaa agtatcaata
     1321 cccctcttat aagtatcttt cttttttatt tgtgcctgca ttgttttatt aagttcagac
     1381 cattccttat tcatattcaa tcctctctca aattctgatt ttatcgcccc atacaaactg
     1441 gaatttgtct tacaacaatt tatatatgca taaaaagcaa tatatttatg aaaacaccct
     1501 aaatccaaaa tttactcaat tcattttttc cttacataaa atgttctcgt atatttcact
     1561 tcctcaccaa gtgtttcccc tatcaaaact tcaaaatcat tgatatcaaa ttctggaaag
     1621 aatgtatctc catcttcaat atttaaatct acttctgtga tatacatttt atctactatt
     1681 tgcaaagctt ccttaaatag tccatatcca ccagatatgt atacatctcg tcctttagcc
     1741 aataataatg catcttctaa tgatttaact gaaactaaat tatctccttg atactctgtt
     1801 gtggtggaaa caacaatatt cattctatta ggcaacggat gaccgatttc ttcataagac
     1861 tttcgcccca taataaccac attacccgtt gttaactctc taaattgctt ttgttctccc
     1921 tttattttcc atggtatatt accattcttg cctataacat tattctttga cctcgcaaca
     1981 atcaaaccta tcatctattt catcctccat aatatgatgt cgtacctgtg attccatcaa
     2041 gtgcaccaca tcttaacaaa ctcaaaatta tctcgaacta accagctcga caaattcctt
     2101 ttttgtcttc atctgcatta tatcatattg ttataagcat tgccatttct tttctgcttt
     2161 tgaaaagtta cttatcagcc tatgttttgt aaagggtgat attcagcatt tctcacgcaa
     2221 caagcccttg acaaaccata gactaataag ttttaaggta atcaagcaga aatgaaatag
     2281 tctgctgttt cacggcaaca aaaaagagat accaacacac ttatgtagta tctcttagtc
     2341 tagtagcaat attcttttgt ttgcaagcag gtaaacatgg gctgtttctt aatatctttt
     2401 tgtttgggaa acaccttttt cccatccttt caccgttgcc atctctgctg cgcacaatgc
     2461 cacagggata aaccatagca aaccgcctaa taacaagaaa aatactaagc tgaagcctga
     2521 ggtcgcgaat gtgggatatt catataatga aacgaccatt gagatggtca tagccaaaaa
     2581 gccaaagaac gttaattttt ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc
     2641 tcttttctat ttgaatttgt ttcaattgag tttgttgcca aagaagaaaa agtccaatcc
     2701 ttcttcttgt tcttcttttg tagtagtgat cgttgtaaag tgcatcgccg attttaaaat
     2761 cactgttgtc agcacatagg catacacggc agtaatgatg acaccaatca cttgaatccc
     2821 taagaagtac cagctgcttg gattcgttag taaatggctg ttagatgcaa agatccctat
     2881 cagaatcgta cctgtgaatc cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc
     2941 ccattgtttc tttttacgaa aatcaacagc agcatgacag acgatacccg caatgatccc
     3001 aatcagcatg gcactttttg cttctacata accggcacaa ggcgtaatgg ctgccaatcc
     3061 agcaacggat cctgttagta cgtcgacaaa atcaaagcca tttcctttta cttttgcaat
     3121 gatcagccaa gtaatcatcg caaatgcaag agcgatgaat gttgaaccaa atgcattgac
     3181 tgcttgttca tctgctcgaa gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag
     3241 cagtcctgta ccgatagcag ctgccataag actgctatgc tgacttcttt ctcctaaagg
     3301 aatctttctt tgaccaagtg tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg
     3361 gatgactgct ccaccagcga aatctctaaa tcccatctga tccaaaaatc cgccacccca
     3421 tatccagtgg caaacaggga aataaatcag cagattccaa aaaataacaa gtataatata
     3481 gccttttaaa ttcaatctcc ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat
     3541 aaacatcagt tggtaaagaa agaacattaa aaatggaatc gttgcaccat gaaacatgtt
     3601 ggggaagctt gtaacatgtc tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc
     3661 gatatcccgt ccaaaggcca atccgaatcc accaaatacc cacataatcg ttgttatccc
     3721 aacggagaga aaagattgag acatgatcgt tagtatatta ttcttccgtg ctaatccgcc
     3781 gtaaaaaaaa gctagaccag gagtcatcag gcaaaccatt gctgtacata aaatcatgaa
     3841 tgctgtatct cctgtattga taggtaatgt attcattttt ttcgcctcct ttctacgatt
     3901 acgagtataa aagaaggtgt agttgttttt ttcttctgtt tttttgaatt aatacttgca
     3961 tttataaaag acagacaatt aataaaagct tgatgtgttg tgctcttttt cgtttttcac
     4021 acaaataaag acaaaaaata aacggtcttc tttttataga aaaccgttta ttttcgtctt
     4081 tttttatttt taaagat
//
LOCUS       DACEPW010000198         4055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEPW010000198 DACEPW010000000
VERSION     DACEPW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.203"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(437..610)
                     /locus_tag="IW819_002434"
     CDS             complement(437..610)
                     /locus_tag="IW819_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAQ1245520.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            complement(953..1642)
                     /locus_tag="IW819_002435"
     CDS             complement(953..1642)
                     /locus_tag="IW819_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1245521.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIGHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1705..2469)
                     /locus_tag="IW819_002436"
     CDS             complement(1705..2469)
                     /locus_tag="IW819_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1245522.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIVKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(2497..3195)
                     /locus_tag="IW819_002437"
     CDS             complement(2497..3195)
                     /locus_tag="IW819_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1245523.1"
                     /translation="MARTQKQKAAQTHVVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTIAANIAVVFAKSGQRVLLVDADLRKPVVYETFQLNNAS
                     GLSTALSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYRN"
     gene            complement(3207..3986)
                     /locus_tag="IW819_002438"
     CDS             complement(3207..3986)
                     /locus_tag="IW819_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1245524.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
BASE COUNT         1117 a          827 c          652 g         1459 t
ORIGIN      
        1 atcttaatta tctatccatt tcatcctacg cattcttgcg taaatacaat tcactctgtt
       61 gagctgatat aaatcattgg tggtacaaag ataaaatgat aactgtttct atacctccaa
      121 aaattccagt aaatatatct atagatagtt tcaaaaaaaa ttgtcttagt ttcataatat
      181 ttaagatagt ggtacatact ttataacaac gtacttttcc aaatattatt ttccgttatg
      241 aacatccaat atattacaaa gtttaaaagt ttagatgaac tataatcttc atttctgcac
      301 agtggataat aaattcttat agataaaaaa tagcctcttc taaagtcaca aataccttac
      361 tcccacattt taagtaaaca ctctgcagca ttctctttgt tataaaatga tagatattcc
      421 tcaattttat ctcttattat gttattatca gttgatacca ttccattaat gtttcagaat
      481 aattacaata ttttgggacc ggataactcc cttttgctcg taaccactca tatccgttag
      541 gattcactaa aagtttaccg tttatttttc gattcttttt ttgaaccgtc ccatgaacat
      601 accgattcaa caagctaaat tataaaaatt ggtgtgctgt cattattttt tggaaaattt
      661 attaatggct atcaatatac ttttgtatca tactaaatag ctcgtcagtt cctatatcgg
      721 atataccaat attaaagcat ttagatcatt atatttaaga gtggtatcat cccaaaattt
      781 cgaatatttt cttttatata agaataatga tactgaattg ccccattcac ctttcttttt
      841 ttagattttc tacaagtgat tcaaaccctc cataattaca gttattactt ttgaaacgat
      901 aatatgaaca tgtgtaatct tttttattaa caatccatca ttctatccct aactaatatg
      961 ctccattggg cttgacaata acataaaacg tttttaaaat caacctcaag tcgtacatta
     1021 aacttcgatg accgatatac tctaaatcca actcaaccat tttatggaaa ctaacattat
     1081 ttctatcact aacttgccac aaacctgaac aaccaggttt tacatacaag cgttgaagat
     1141 catatctcgt gtactcagct acttccctct ccaaaggtgg ccgtggacca attagactca
     1201 tatcgccttt taaaacattc catagctgtg gcaattcatc aatgcttttg gcacggataa
     1261 ttttacctat tttagtgaca cgcggatctt ctttcatctt gaacattgca ccttctattt
     1321 cattttgatc aagtaattct tccaattttt cttcagcatc taaacacatc gaacggaatt
     1381 tatgcatttt gaatgttttt ccatttttac caacacgtgt atggctaaaa aatattggac
     1441 ttcctggctc ttcttttctg attcgataag ctacatagaa catcaaagga gaagctaaaa
     1501 tcaaaccaat aatgcttcct acaatatcaa tcattctttt aaaaaataaa tagatatatc
     1561 gtttttcaac tttttcttta tccaaccaaa cttgattagg atgtacttca ctaatacgag
     1621 ataataaatc ttcactttcc atactttcag tccctttcac tatcttcact ctctcctcat
     1681 ttgtttttat ctacgaataa agaactaaaa taaaccaaac ttcttctttt ttacctcttc
     1741 atagattgga taactgatct catccccgtt gatcagatcc tttgcgactt gtttcatctg
     1801 tactactttt tccttaccaa agtccttgac aatctgctca tagcactctt ttaagtaaaa
     1861 ggtgcgctta gagaccccgt gtgcatcaga agcagccatc tgcacgagat tatgtttgac
     1921 cattgttttg gctgttttct ggatgtcttt gccaaataca ccaacataac ttggcgcagt
     1981 aatttgggcc aaacatccca tatctaggaa cggaagcaaa tgatttgggt cttctcgaaa
     2041 atgcgcgttg cgttctggat ggacgatcac tggtgtcttt cctagttcta acaactcaaa
     2101 aaaaagccat tctgcatata acggcacttc cagtgtcgga aattcgatca gaagatatgt
     2161 atcatctaaa tctgtaaata aaatttcatc tcggtggatg tcttcgatga gcgttccggt
     2221 aatacgtact tcttgtcctt ccaataacgt caaaggcaac tgtcttttct ctaattcggc
     2281 ttgtaacgaa gcgactagag aaatgacttg tgatttttca ttgctgtatt ttccattatt
     2341 gtggtgtggt gtgcataaaa tatgcgtgat gccttgctga atcgcttttt cagccattgc
     2401 aatacttgct tctaagtttt ctgccccgtc atcgattcct ggtaggatat ggcaatgtag
     2461 atcgatcatt tctcttgtct cctctcttct tattccttaa tttctgtagt aataaccagc
     2521 atctttgcta tgttctgctc cgttgtatac aacacctaag actcgtgctt gtaccatctc
     2581 tagcagttct tttgctttag tcaaggattc tttacgtgat gtattttcac gtacgaccaa
     2641 taaagtgcca tccgtttttg aagacatgat ctgtgcatct gttacggcta ctactggtgg
     2701 catgtcgaag atcactacat caaatagttg tcttgcttct gctaaaatct gatccattct
     2761 tggcgagctt aataactctg atggatttgg tggtttaggt ccacttggca gaatcgacaa
     2821 attctcgact ggtgttcgtt ggatctcatc tgctacactt cctgatgaac taagtgctgt
     2881 acttaatcca ctggcattgt tcagctggaa tgtctcatag acgacgggtt tacgtaaatc
     2941 tgcatcgact agtagaactc gttgaccgga tttagcaaaa acgactgcaa tgtttgctgc
     3001 aatggtggat tttccttctc caggacctga tgaagtcacg acaatcgttt tgatttgctg
     3061 accagctgcg gaggcaaact ggatattcgt ccggatcgta cgatattgtt cggcgattgg
     3121 tgaagatggg tttgttaatg tgatcaaact gactacatga gtttgtgctg ctttttgttt
     3181 ctgtgttcgt gccatttttc ttcctcctag actcttgagc ggcttctgcg tgatggagct
     3241 gttttatcgt ttgtagcttt tggtcgtgca ggagctgatc cttttttacg gataccagcg
     3301 ttcaattctt tcgctgacat ttctggaact gttcctaaga ttgggaatcc taatgtatcc
     3361 gtcacatatt tttcatcttt cactgtccga tcaagtaatt cgaataagaa tgccaaacct
     3421 acgcctacca tcattcctaa tactaagcca atcactagat tcaatttatt atttggcgaa
     3481 acaggtgtcg tattggcaac tgcacctgag atgatcgaga ttttatcgac attcatcacg
     3541 tcttttgcgt tctcttggaa aacttgtgaa gttgtattag cgatctgttg agcagtcact
     3601 gcattcgtgc ttattgcctg gatcgagaac atttgtgagt tttctgactg attgacggag
     3661 atcgcatctt tgatttgtcc agcggtcatt ttcacattgt cttctgtttc tagacgatct
     3721 ttgacttcat ttaatactag atcactaacg atcatatctt tatacgtatt gatcatttgc
     3781 aggttcgtat tgacatcatt gacattcgct gtgtctgatt gcggcagtgt cacgatcaat
     3841 tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc
     3901 aagccagcaa acattgagat gatgattgtt gccaagtgtt tgcgtaatgt tccaaaaatt
     3961 tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt ctctagattc
     4021 atttgatctt ttaattcttt tttcacacga tcaag
//
LOCUS       DACEPW010000199         4021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEPW010000199 DACEPW010000000
VERSION     DACEPW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.204"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            36..308
                     /locus_tag="IW819_002439"
     CDS             36..308
                     /locus_tag="IW819_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245525.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            475..876
                     /gene="tnpA"
                     /locus_tag="IW819_002440"
     CDS             475..876
                     /gene="tnpA"
                     /locus_tag="IW819_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1245526.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            888..2033
                     /gene="tnpB"
                     /locus_tag="IW819_002441"
     CDS             888..2033
                     /gene="tnpB"
                     /locus_tag="IW819_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1245527.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            2271..2534
                     /locus_tag="IW819_002442"
     CDS             2271..2534
                     /locus_tag="IW819_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ1245528.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            2528..2878
                     /locus_tag="IW819_002443"
     CDS             2528..2878
                     /locus_tag="IW819_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1245529.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(2902..3516)
                     /locus_tag="IW819_002444"
     CDS             complement(2902..3516)
                     /locus_tag="IW819_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1245530.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         1445 a          598 c          725 g         1253 t
ORIGIN      
        1 ttagattatt ttgaaaatat caataagtat ggcgcatgtt acatcatgaa gtaatcagac
       61 ggattcaaaa agactttagt tgtatggaag aaatgaagta ctttgttcaa aatgttgaag
      121 aacagttata tttggaaagc gaaacgagtc aaacaaaggt cacagtcagc cataatcgtt
      181 tagatgtgta taaacgtcgt cctgaatcat taaaaaaagg agcggagctt ctacgactag
      241 aaacagaaaa atacgtcatg aatgtttacc tagaaaaaag tgagttggtc gaaaatttta
      301 tggactagcc gagaacaccc gtgattttaa tcatgggatg aatcggattc agttgtgacc
      361 tagctttagc taggttaaac aactgacaaa tataaactaa ataatagtga aatgtcttgc
      421 aataattgat tttgtagaaa tacatatatt aaaataattg cgaggtgttt ttgtatgtca
      481 gaaattcatt atggacgggg ttatgtctat tctattcaat atcatcttgt ttggtgtgta
      541 aaatatcgac atgatgtttt gtatggtcag gtagatattg atgtcaaaca gtttttgaaa
      601 caaattgctg atgataataa tataaaaatt atagaaatag aatcagataa agaccatatt
      661 catttactca ttgaatgcac cccacaacac tatattccta gtattgttaa agtatttaaa
      721 ggcgcatccg ctagactgct gtttaaaaaa catcctgaac ttaaacaact cctttggggt
      781 ggacatcttt ggaatccaag ctactttgta gctactgtta gcgaaaatac agaagaacaa
      841 attagaaaat atatacaaaa tcagaaaaag aaatgaggtg aatccgtatg ctacaacata
      901 gggcttataa atttagaatc tatcctaatc aagaacaaga aatattaatg actaaaacta
      961 ttggctgttc aagatttgtc tataattatt ttttgaattt gtggaatcat gaatatacga
     1021 ctactggaaa aggattatct tataattctt gttctgccat gcttccacaa atgaaaagaa
     1081 atgcagagac aatttggtta aaagaagttg acagtattgc gattcaatcc tcattgaaaa
     1141 atctttctga tgctttttct cgcttcttta aaaagcaaaa taaacgacca caatttaaaa
     1201 gtaaaaaaca tcctatacaa agttatacaa ctaaaaatgt gaataatagt atcgaaatta
     1261 gaaacaattt cgtaaaacta cctaagttag gatttgttaa atttgcgaaa agtcaagagc
     1321 ccaaagggcg aatcttaaat gctacgattc gaaaaaattc gagtggtaaa ttttttgttt
     1381 ccatcctgtg tgaagaagaa atagttgagt ttcctaaatc taattccgct attggaatcg
     1441 accttggtat aactgatttt gcaatccttt ctgatggtca aaagattgat aacaataaat
     1501 tcacgtcaaa aatggcaaaa aaactcaaac gtgaacaacg taagttgtca aaacgtgcat
     1561 tactagctaa aaagaaaggc gtcaatctct ttgaagctaa aaattatcaa aaacaaaaac
     1621 gaaaagtagc acgactacat gaaaaagtaa tgaatcaacg caatgacttt ttaaacaagt
     1681 tgagcacaga gattatcaaa aaccacgata ttatctgtat agaagactta aatacaaaag
     1741 gtatgttgcg taatcataaa ctagcaaaaa gcatttctga tgtgtcttgg tctagttttg
     1801 tgactaagtt acaatacaaa gctaactggt acggtcggaa aatcatcaaa gtagataaat
     1861 ggtttccatc tagtcaaatc tgttcaaatt gtggacacaa agacggcaag aaatctctag
     1921 aaattcgaga atggacttgt cctgtttgtc atgctcatca tgaccgagat atcaatgcta
     1981 gtatcaatat tttgaccgaa ggtctaagat tacatgtcat gggattagct tagatccaat
     2041 catcaaaaac cgtaggaact acggggatag cttggtaaat aagagaaacc gctgtgagta
     2101 aagaaatacg cttgcaagta tgctctattc ccaagaatct cgtgacttta gtcatgagag
     2161 gttcaaggat tcatggaatg ttaatctaat ttaatagaaa aggataatta agtatatacg
     2221 ttttttctta tgtatgttat actatgtata tacagaaaga ggtgtctact atggaaatgt
     2281 tacctataaa aaaatgggga aatagtaatg gccttagatt gccaaagtat attatggaat
     2341 atcttggaat tcatacagaa gacaaagtga agataataca agaagaagta aacggtcaga
     2401 agcggctaat tatcgaagcg gctaatatag aaaatgaatt aacaattgaa cagttatttg
     2461 aaaattacac tgatgaaaga aatcatgtga cgattcaaaa tttaggtgaa gcagtaggta
     2521 atgaaaaatg gtgagaaaac ctagacaagg cgatattcta ttattgaata cagcacctcg
     2581 atctggacac gaacagacag gtaaaagacc ttatattgtt ttaagtcatg atattattgc
     2641 ggattatagt aacgttgtta cagttgctcc aatttctagt acaactagaa actatccgct
     2701 atatgtaaat ataaactcag aatatgaaat gaagacaaca ggaaaagtat tattagatca
     2761 actaacgaca attgattatg aagccagaga gtgtattttt ttggaaaaag cgcatgatag
     2821 tttagttgag gagttattga tgaaagttag aacagttttt caaaaagtaa gcaaataaaa
     2881 tagaaacagc agcatgatca actattcgtg attgaccatg ctgtcttttt ttattctata
     2941 tacggtttgt ctcgtaacat tgtagtcttt tgcaatctta gcgatcgcaa cacctttttt
     3001 tagatcctca acgatgtttt tataaacaag acgacgttgg ggatcttttg cattggcact
     3061 gtatagctta ggacgtcctt tataaacacc gttcgctttt gcaatcttaa tgccttgtgc
     3121 ctgacgtcgt ttagattcgg tacgttcttg ttcagcgatc atggccaaaa tttgaatgat
     3181 caaatcttta atgaatttat ccaggagagg attgccaatt gcttcggcca tgatcggtag
     3241 acttgtgatg atcagctgca catttttctt cttcaagtaa ttgactgaag cgatgatctc
     3301 gtcgtaattt cttccaagtc gatcaatagc ttccacaata aaaatatctt gatctcggac
     3361 gaaatccagt gcttcttgaa aaacaggtcg atgagtaatc gttgcgccag attgtttctc
     3421 aatgaaaatt ctctctgcac cgaatttttt catttcttca atctgccgtt gttcattttg
     3481 atcgatggat gagacacgta tgtatgcaac tctcaaaatt tttacctact ttctgcaact
     3541 atttaaaaaa caccctaatc atacacgaga aaaccagatt tttcaatgtc gcggtcgtgt
     3601 ctagttaggg tataccttat atagacaaaa actaatgatg gaggaaatag tatgagaatt
     3661 ttttgtaagt gtctactgat aacttattct caaatgttgt aaggatttta ttcatttgtt
     3721 aatattaaag aacggaactc caaaaaagtc cgtactctaa tttgcagtct ctcactcttt
     3781 tagagtgaga gtttttatta gattttcaaa ttaattagta aagtgataga ataaattgat
     3841 catacaaatc aaaagaagaa gcagggtgat agcataccta tgcttctttt tttatgtatt
     3901 tagctatttc ttctttacaa tatcgaacat atgtttgtat aatgtgaaag aatggagtgt
     3961 tatttatgat ggattattca aaagagccag taaatgatta ctttttaatc gatatgaaaa
     4021 g
//
LOCUS       DACEPW010000200         4010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEPW010000200 DACEPW010000000
VERSION     DACEPW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.205"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..552
                     /locus_tag="IW819_002445"
     CDS             <1..552
                     /locus_tag="IW819_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245531.1"
                     /translation="DELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDD
                     TNVKLVRPFGDMMGLHEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYF
                     GYNVDTGRNVYLKPALASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQ
                     AVIVDPKGGAKRSYLKRVGTALH"
     gene            1249..3075
                     /gene="ltrA"
                     /locus_tag="IW819_002446"
     CDS             1249..3075
                     /gene="ltrA"
                     /locus_tag="IW819_002446"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1245532.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            3209..>4010
                     /locus_tag="IW819_002447"
     CDS             3209..>4010
                     /locus_tag="IW819_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1245533.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1346 a          700 c          910 g         1054 t
ORIGIN      
        1 gatgaattgg aaaccacctt ggatcagtca aaagaatcta tgtacaaact gagttatgtg
       61 gttcgagtga gtgccgaatc agtggatgaa ctaaagcgac gctgtgatga agtgctcgat
      121 ttttatgatg ataccaatgt aaaactagtc cggccctttg gcgatatgat ggggctacat
      181 gaagaattct tacccttaag taaacgatat atgaacgact atattcagta tgttacgtct
      241 gattttttgg cggggcttgg ttttggggct actcagatgc taggagaatt agaagggatt
      301 tactttggct acaatgtgga tacaggtcgt aatgtttacc tgaaaccggc attggcttca
      361 caaggagtca agggttcagt aaccaatgcg ctggccgccg ctttcctggg ttctcttggt
      421 ggaggtaaat cctttagtaa caatctctta gtctactatg ctgttttgtt cggtggtcaa
      481 gctgtgattg tcgatccaaa agggggtgcg aaacgttcct acttgaaaag agtaggcaca
      541 gcacttcatt aaatcaatat ttaatgtgtg ttggagaact aaacacacga atagtcgaat
      601 gacggggtaa cgccctgaaa ggctacttta atactctgac tggcagaaac ttgagtaaac
      661 aaggctctgt cagaagctcg gtgaagtcgg cagacagaca ccgtaacaga taatgctgac
      721 gaaagattcc cagaggtggg cgttgtgtcc tatatgccgg aagtctataa aatatctatg
      781 gttagaatgt agatgagagg tctactgacg aatccttgaa tgtacaggtc tatatcgggg
      841 ctggatagaa atgtccagtg acacaaacag tgtcgctact gtgggtgagt aagactccgc
      901 atttatgaaa atccatataa cgttaaaggc gttatcaagg tagcaggctt atagaggaaa
      961 cctaaggata tatgtacaga tagtgtgttt ggaacgttga aagctgacaa catggagatt
     1021 ttactgtctg tgaagtgttc acatcagaaa gttattgtga gattagcaat gatataaggt
     1081 gtgttaatgt cagtgagagt ggtggcacag tacctatgaa accgtgataa caagcggtgg
     1141 agggatagcc actagtcata tgaagaatag aattactaag aaaaatggta tgggttcgag
     1201 taagactaag agatgtactt gctcctgcaa acaaggaggg tacagaccat gctaaagaaa
     1261 aacaagctcc gctataacga gtattatgat atgcaacata tttatgacga gttatacgca
     1321 cagagcaaga acggtaataa cttttataag ttacttgaaa ttattggttc ggaacaaaat
     1381 atttgtttag cttaccgtaa tctgaaatca aacagtggca gtaaaacggc tggaacggat
     1441 ggaatgacga tagacgatat aaaacaactc agcaatgctg agattgttgc taccgttcgg
     1501 gaaagcctta gcaactaccg ccccaaatca gtcagacgtg ttttcattcc aaaagctggg
     1561 agcgataaaa tgcgtccatt ggggattccg tgtatctggg acaggctggt acaacaatgt
     1621 atcctgcaag tcctagagcc gatatgcgaa ccaaagttcc ataatcattc ctatggattc
     1681 cgtgcgaatc gaagtgccca ccatgcagtc agcagagtaa cgacactgat aaatttatca
     1741 aaatatcatt actgtgttga cgtagacatt aaaggatttt tcgataatgt caatcatgga
     1801 aaactattaa aacaaatctg gacacttggt attcgggata aaagattgat ttgcattatc
     1861 agcaaaatgc tgaaagccga aattgacggc gagggtgtcc cagaaaaagg aacgccacaa
     1921 ggcggtctgt taagtccact gctctccctc atagtcctta atgaattaga ctggtgggta
     1981 agcagtcagt gggaaacatt ccagccgaag aaccgcagta aaaatgggtg gcttcaatac
     2041 gcaaagaaat ataccaaact gaaaagcggt tttattgtac gttacgctga tgattttaaa
     2101 atcatgtgtt caacctatgg agaagcacaa agattttacc acagtaccgt agattttctt
     2161 aataaaaggc tgaaattaga aatcagtcca gagaaatcaa aggtagtaaa tctgaagaaa
     2221 aattcatcag attttctggg gttcaaaatc aaagtcattc caaaaggaag aacgaaacac
     2281 ggttatgttg ccaaaacaga tatgaaccaa aaagcattaa agaaagcgaa aacaaatcta
     2341 aagttaaagg taaaagatat agccagacat acgactggct tcaatatcag cagatataat
     2401 ctgacagtta tcggtatgca gaattactac tgcatagcaa ctaacgtata taacaacctg
     2461 acagaagtaa gttacgctct cttaccgacc atacgaatcc gtttaaggaa cattgcaaaa
     2521 tcagttccat ttgaaagtac aagttcagaa tttcagagca ggacaaaagg aatcagacca
     2581 aaaacaaaaa tagtgatgat tgcggataat ccgctattgc cgattcaagg agtacaacac
     2641 aaaaatccta tgaatttctc gcaagatatt tgtaatttta caaagcaagg gagaaacaag
     2701 gtgcatgagg acgttgtagt tgtaacaaaa gaagaaatcc gagcgttgtt ggaaaatgaa
     2761 aaccctgccg atagcgtaga gtttaacgac aatcgtattt cggcatatat cgcacaacaa
     2821 ggcaactgct atgtaatgaa tcgtcgagga actccgtcca ctctgatatg tattcacaaa
     2881 tcagacaaag gagataacct tgacagatat tctaatctgg catttgtgga aattcccatt
     2941 gcaaaggcaa tcctaacaga atcagcagac gaagcaaaat cactgttaaa aggttatgtt
     3001 cttaatagtc agcagaagaa aaaactgaac agaatacgga cgaattacgg ataccagaca
     3061 ataactggga aataacttat aaatctattt ggatatgatg gaacgccgta tgaagcgaaa
     3121 gtttcatgta cggtgtgaag cgggggaaaa cttggagata acatcaaaaa gttacctatc
     3181 gctatagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     3241 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     3301 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     3361 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     3421 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     3481 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     3541 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     3601 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     3661 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     3721 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     3781 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     3841 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     3901 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     3961 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DACEPW010000201         4007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEPW010000201 DACEPW010000000
VERSION     DACEPW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.206"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..779)
                     /locus_tag="IW819_002448"
     CDS             complement(<1..779)
                     /locus_tag="IW819_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1245534.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNV"
     gene            929..1489
                     /locus_tag="IW819_002449"
     CDS             929..1489
                     /locus_tag="IW819_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1245535.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINDFIATLP"
     gene            1618..1995
                     /locus_tag="IW819_002450"
     CDS             1618..1995
                     /locus_tag="IW819_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1245536.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            1928..4000
                     /locus_tag="IW819_002451"
     CDS             1928..4000
                     /locus_tag="IW819_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1245537.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLNLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         1452 a          767 c          814 g          974 t
ORIGIN      
        1 acattcccat gtccatgcag caattggcga atgtacagac tgatgattgg actaatggaa
       61 ttgttggatg cttggatgat catcgttgtg acgaacatac cgatgacgac gtgtggatat
      121 ttcaagtctt tgaaaatctg tttggcagaa accatgtttt cttttttcac tgggacaaat
      181 ttttcatgga cgaaaaacag actcaacaag aagacgaaaa ataagatgat ccctgtaatg
      241 aaaaaggtag ggcgatagcc aaacgctgaa gcagagatcc cgccaagaag cggtccaagt
      301 aatgttcctg taacagaacc agttgctaag gttccaagaa cttgaccact tttttctttt
      361 ggcgtcccag ttgcgacaag ggcagtggca ttactgatat aaccagaaaa aatcccttga
      421 agcagacgca atgcgactaa ttgatacaca ctagtcacgc aacccatcaa agaaatgacg
      481 attgccattc ctaatgaagc acgcaacagc atcaattttc ttcctttttg gtcggctaat
      541 tttccccacc aaggagagat cagcgtagtc accaagaaag tagatgaaaa agtcaatccg
      601 ctccaaaagt tcaattgtga cgtactataa ttacctaacg tatctatgta aagtgacata
      661 aaaggcatga ctaaactaaa tccgatacca gccataaatg ttccaaacca taagacaaac
      721 aagttttgtt cccaagtttc tcttttccta aataattttt cctttattct tgcagccaat
      781 tagactcctt ctttctgaaa attttcatct acagatcttt attataaact acaatatgta
      841 gtttatcaat gaaagtctct tttttctctt tctttttcgt ggtaaactga aagagtgaag
      901 gatttatttt atgagaggaa acatggaaat gaaaaaaaga aatctgtctc aagagaaaat
      961 catcgactgc tttcgagaac tagcagaaga gatggatgtt cagcaaataa cctttcagca
     1021 tttagccaaa cgcttggata tcaaggctcc ctcgctgtat aaccatttca aaaatattcg
     1081 tgatgtcaaa acagcgctaa cagcgaaact tctgcaggaa cttaatgacc agctgcgtcg
     1141 ggctcttgta ggaaaaagtg gtcaggaagc actgcagatc tatgcagaaa cttatcgatc
     1201 ctttgctttt tcaaatcaag cagtgtatga gttactgatc agtgtaccgc ataccaatga
     1261 agctgtttta ttagatggaa tccatgaaac gaatcagatc attttgcaga tatttgaggc
     1321 ttttcaatta acgaaagaag aaaaagttca tcgaagcagg gaacttcgta gtatgatcca
     1381 cggttatctt tccttacgtt ttttaggata ctttacaaaa gaaccaacaa tcgatccaga
     1441 agacagctat ttttggatga tcaatgactt tatcgctact ttgccttaaa caatcagatt
     1501 catttgtaac aattttaaga tgttcattaa atgacccttt tataagagaa aattcttgca
     1561 actttcttga aaagtgacta agatgagtct agaaaagctg aaagaaggag cacgagtatg
     1621 tatcaagtaa ttaccatgtt cggggacaat gagccttggt ggttttttga agactgggaa
     1681 gaagatattg aagcagaaga aacattcgag tctttcgaag aagcacgaca agcgtacgaa
     1741 aaacaatgga acgaaatcca tcaaaattat gaatatatca atgcaaaaag taactttctc
     1801 agcgcctttt ggaatgacgg agatgagcgc tggtgtgaag agtgtgacga tgatttacag
     1861 caatacaaag gacttgcgtt gttgaaaaat catcagccca tcacagtaga aagcggaaag
     1921 gaattttatg aaacaactaa ttctagcgga aaaaccaagc gttgcgaaag acctaagcaa
     1981 ggtgcttggt gctaatcaaa agcacaaaaa ttattatgaa ggacccaagg tgatcgttac
     2041 ttgggccctt ggtcatttat taggattgaa aatgccggaa gatctgaata aggaatggca
     2101 gagctggcaa atggacacgt tgccaatgat ccctaaaaaa ctaggaatca aaccacttcc
     2161 taaaacagga catcaattaa aagcaatcaa gcagctagct cagcgaaaag atgtctcaga
     2221 agcagtcatt gcgacagatg ccggaagaga aggggaactt gttgctcgct ggatcttaga
     2281 gtatgtgcat ttcaataaac cagtgaagcg gttatggatc tcttctcaaa cagataaagc
     2341 aatcaaaact ggattcaagc aactaagacc tgcgaaagaa tatgatgcat tgtatgaatc
     2401 tgctcttgcc cgggcaaaag cggactggct ggtaggattg aatgtgacca gagccttaac
     2461 tgtgaagtac gcggataatt tatcagcagg ccgtgtgcag acaccgacac tagcattagt
     2521 gagagaccaa gaaaagaaaa tcgagcagtt ccgtccacaa acgtatttca cgatcacgtt
     2581 gcaagctgga aaagaacaag ccaaaatgct tcaaaaaaat caatttgctt taaaaacaca
     2641 agaagaagca caaaaacttg ttcaattgat cagtacaagc aaaggaactg tcacagaagt
     2701 ccaagaaaaa ttaaaaacgg aatctgcgcc attgccttat gatttaacag agatccaaag
     2761 agaagccaat caacgctatg gctattctgc aaagaaaaca ttaaatcttg tacaaagttt
     2821 gtatgagacc cataagatcg ttacttatcc gcgtacagac agcaaatatc tgacaaatga
     2881 catgaaaagt acgatgaaag aacgattgca agcagtcagc gactttgctc ctgaggtcaa
     2941 gcagtatttg aaaaacggag ccatcgtcca acagacaaaa gtattccaag acagtaaagt
     3001 gaccgatcac catgcacttt tgccaactga aaacagagca cgttatgaaa aattaagtaa
     3061 tgaagagcaa aaaatctatc aaatgattgt ctctcgtttt ttaggactgt ttgctcaacc
     3121 ccataaagtc agccaaacaa aagttacggt tgaatttggt aaagaacgat tcatttttcg
     3181 gcaaaatcgt gtcgtccaag caggatggaa aggcgaaact gaaactgaga cggaaacagt
     3241 caaatgggaa aaaggaatga gaatcaatcc cgatttcgcc atcaaaaaag aactatctgc
     3301 accgcctaaa ccattgtcag aagcaagctt gcttggtcaa atggaaaaat acagtctggg
     3361 aaccccagct acgcgtgcag agattatcga gaaactaatc aaatctgaac tgatggaacg
     3421 tacccctcaa ggactgcaag tatctcctaa aggtaagcaa ttattagtat tggtaaatcc
     3481 ctctttagta acaccagatt tgacagaaag ctgggaaaaa gaccttgaag ctattgctgc
     3541 aggaaagaaa caagcaggaa ccttcttgaa tggaatcgaa aaagaaacaa aacgcttggt
     3601 gaatgagatc aaatcaagca aacaagaata tcaagatttc tcgattaccc aaaagaaatg
     3661 cccagaatgt ggggccaatc tgcgcgagaa aaacacacgt gacggcaaaa tctatgtctg
     3721 caccaattca gattgcaact accgcagaag aaaagatcca aaagtctcta atcatcgatg
     3781 cccgcaatgt caccggaaaa tggaaatcat cgaaggtaaa aacggtgctt atttccgttg
     3841 taaatacgat ggaacgacag aaaaaatgct tgataaaaaa gagcagaaga agaaaatgac
     3901 caaacatgaa gaacgacgct tgctgaaaaa atatgcacaa gcagaagaac cagaagaaag
     3961 cccattagca gcagcactaa aagcggcaat gaagaaataa taagata
//
LOCUS       DACEPW010000202         3992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEPW010000202 DACEPW010000000
VERSION     DACEPW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.207"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(112..387)
                     /locus_tag="IW819_002452"
     CDS             complement(112..387)
                     /locus_tag="IW819_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ1245538.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(359..1312)
                     /locus_tag="IW819_002453"
     CDS             complement(359..1312)
                     /locus_tag="IW819_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1245539.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1924..3417
                     /locus_tag="IW819_002454"
     CDS             1924..3417
                     /locus_tag="IW819_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ1245540.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3552..3848
                     /locus_tag="IW819_002455"
     CDS             3552..3848
                     /locus_tag="IW819_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1245541.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(3872..>3992)
                     /locus_tag="IW819_002456"
     CDS             complement(3872..>3992)
                     /locus_tag="IW819_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1245542.1"
                     /translation="FIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1327 a          653 c          717 g         1295 t
ORIGIN      
        1 tgttgtatac ccccaaacaa tacacatatt ttgaaggatt aggattaata aagctaaaaa
       61 tatagtaagt atactaaata tatttactat atctagtata cttactatat tttaatcttt
      121 tgatcgtcta ttatatataa tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat
      181 tgcaagtttg actatttcat acataggaat atcttttgca taactaagat cccgaatggt
      241 ttttagtacg ggaggatcta cttgaactgg ttttctttct ttcgcagtgt actttcgttc
      301 ccctaactcg acattaatat catctttaat tgaaatatct tcagcaccaa ttttcttctt
      361 atctttgttc gttggtaata atgccattta gtttaatctc ctttccaaga ggtaggatat
      421 gattggccat tgagtcagca tatttaggtg tataatcaaa tccttcaata tcaccatgtt
      481 gttcatagta ttcaattctt tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc
      541 atcttcgatc tacataatca tttaattgga taccattctc accatacatt tctaaccgat
      601 ccgagccttt aacaatgctt ttaaaaatgt ttgattcacc atacttatca attgtttttt
      661 gatagtttgc gtgttgagag gaacgtcgca ctgagaaaag aacaggcaaa attccaatga
      721 tttgaagatg agactcttca aaattcgtga caatcggaac taaaacttta ttaataaaat
      781 cttctgttcc ttccattgcg taccgtttta attcttgcat tgcaataata taatctgcag
      841 cagttaagaa tgcacgaacc acattatctg ttgatggtgg gcagtcgata aagatataat
      901 cgaaattttt tctcaattct tctaaaggtt taatgaaggc caatgatctc ttacgacgat
      961 caggatattt ttcggacaag tattcattaa gttcttcaga tccaggagag ccagccataa
     1021 tatataaatg tggacttact tgagtaatac ctgaaaccca gtcccctttt ttgattgctg
     1081 cagacatgga tcgatcaaag ctagtaattt gatacgttga tccaatgttg gaagtagtat
     1141 ttctttgttg gtcataatca attagcaaca cgtctttttc aaaaagttga ctagaaacaa
     1201 tcgctaataa atcattgtct gtagtcttac caatgccgcc ttttctacct gtaagcatta
     1261 caacttttcc tagtttatct gatgttttaa atgcagtccg taagttttcc atagttccct
     1321 ccaatttcct atactaaata tatttagtat acttcatata cttaatatat tcgatatatc
     1381 taatatactc caaattgata agaattcaga ctttataagg aatatagtgg atatactaaa
     1441 tatatttgct atattcagta tatcagatat attcaaaatg tcaaaatata tttgtgcgtt
     1501 ttgcgttgac aatttagagt gaaaagaggt aaacttaatt ctatagaaac cacagataat
     1561 tcactattta caaagtagca aaagcagtgg tactctttaa acagaaaaat tacataaaaa
     1621 aaagagacgg aaataattga ttttagcaga atcaattatt tctgaatcac atggttagga
     1681 catgtgttca cgctcgtctc aagaatatgt aagtagctac caactactta tattaaaagt
     1741 tgatcggata ccttttccga ttgcctttat tctaccaaaa aaaggcgagc aactcaacag
     1801 aagaacgttt atttagtgta cttaaattaa atattcttaa acactgaaga gagaacacat
     1861 actatggctg agattaaagc taaactgtgt tctctttttg tttgagaaag gacgttacaa
     1921 tcgatgttga gcgaaattta ttcagctatt cttaaaaacg gcttacgtac atacaaatat
     1981 cgaaacagca ccatgaaacc tgcaggtcat attgattcca gtaggagtgg tgctatcttt
     2041 gcgtaccgta gcaagcaatt aatgaatgtt gggcgtggta tggtcattac ttcggaagaa
     2101 gctattttag agaacgaaaa gaaactaacg cattggacac ctaataccta tcgctttggt
     2161 acttatgcgg atgattatcg aacagtggtt aaaggtcatt ctgagaaaaa cttaagtcaa
     2221 atcaacacat ttgtagttga tatcgattca aaagagaacc atcaaggcga aattatttta
     2281 gcctgtttag atcaagtggg ctacatgcca acgctaattt tagaatcaga tcatggttat
     2341 caagtttatt ttgtactaaa gacaccaggc tatgtcacaa agaaaagcaa tttcaaggtg
     2401 atcgatgtag caaaaaaaat ttcaaaaaca attcgtctac agttggctga aaaattgtcc
     2461 ggagtggatc taggatgtaa ccacttcgga attgctcgat tcccacaaga aaataatatt
     2521 gtttttaaag aaatgaacta tcagtattct tttgctgact ggattcaatg gtcgatgaag
     2581 cttgaatcag atcaaaaaac aaaagaagat cgagaacgaa aactaattgt ctttcctgag
     2641 aaaaaagaaa ttcgtcaagt agatgaaccg tggttcgata tgttacttca taaagcaaac
     2701 atcgtcggcg gagaaggacg tcttggtcga aataatgtga ttttcacgct ttctttagct
     2761 tattactctt caggttatgc attggatact tgcgaataca atatgttcac attcaacgaa
     2821 cggttacagg aaccgctatc tgaagcagaa ttagtaaaga ttgtacgtag tgcttattct
     2881 ggtaattatc aagcagcaag cagagaattc attattgaat tgtgccagca atgggtttca
     2941 ccaaccttaa aaaataatga tctgttcatt caacgacgtg gctggtggaa atttaaaaag
     3001 ccacgagatc aaagaaaata tagtcataaa aatgaatgga aagaagatct attggcttat
     3061 ctaagcgaaa aaagtcttgc tagtgagcct tatttgaacc tgtcaaaaaa agaattggct
     3121 tcacaactaa cgatgcccaa gcgaacactt gataagatct taaatgagtt acgagaggag
     3181 aataaagtct tctacaaggt aacacgagga agaaatggcg gaatgatcat tgcttcagtc
     3241 aaaacgttgt tcgcgagggt tattcaacta agtaaagaaa agagactaga ttattcaaca
     3301 aggatcacag aggtatttgg actcaacgag agcttagtag aagagacatt tagacaatta
     3361 tcaaccccct tgaaccctct taaagaagtg aatctttttg aagtagatac aggttgaaaa
     3421 ataaaaggtg caaattgcct tatacaatat atctatgata cttctgtagg tgttgctccc
     3481 tttgcctagc tacgtgtagg tggtatactt ataactgcgt atgcagtctt atgtatcatg
     3541 gaggaagttg tatgtcgtta aaaaattatg tctattcaca tcctgttaat gtgtttatca
     3601 ttggtcgttt gggattatct gtggaagtgt tctgtgagct ttatggtttt aagcagggga
     3661 ctttgtcttc ttgggttacc agggaaaaaa cagttgcttc tttgccgatt gagtttcttc
     3721 atgggttgtc cttagcttct ggggagacta tggatcaggt atatgattgt ttgtgtgtgt
     3781 tagagcagga gtatattgag tataaaatag ctaacgagtt aagaaaaagg aagaagtata
     3841 tccagtagta gtttttattt actcttaatt gtcaaattaa gttagacaaa tcatcaatac
     3901 ttacgtaact caaaaatact tccaaaggtg ttcgataatt cagtgatttt ctaggaatat
     3961 tatttcgttt cgatgcgaca gattgaataa aa
//
LOCUS       DACEPW010000203         3981 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEPW010000203 DACEPW010000000
VERSION     DACEPW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.208"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..899)
                     /gene="prs"
                     /locus_tag="IW819_002457"
     CDS             complement(<1..899)
                     /gene="prs"
                     /locus_tag="IW819_002457"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1245543.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMG"
     gene            complement(979..1854)
                     /locus_tag="IW819_002458"
     CDS             complement(979..1854)
                     /locus_tag="IW819_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1245544.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            1984..3891
                     /locus_tag="IW819_002459"
     CDS             1984..3891
                     /locus_tag="IW819_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1245545.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNEQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIQYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
BASE COUNT         1239 a          822 c          743 g         1177 t
ORIGIN      
        1 cccattaatt ctgaacatgt aacgattgtt aagttttctg gatgacgatt ttcttctgtg
       61 aaaacagaat cagtgataca aatatctttg attggtgcat tgtctaaaat acttttcgca
      121 tgatcggaca ataatccatg tgacgcacag gcatatactt cttttgcgcc gttttctttt
      181 aatacatttg ctgctcgtgc aagcgttgct cctgtgttca agatatcatc taccatgata
      241 catgtctttc cttgaacatc accgatcaca taaccaccct cagttgaagc atcatctgca
      301 tgatctacga tggctaatgt gctgttcaaa tattctgaaa gactacgtgc acgctgaaca
      361 ccgctgtttt ttggcgaaac aactacgatg tcatctcccg tcagacctaa ttgacggtaa
      421 taatgagcaa acaacggcat cgtaaataaa ttatctacag gaatatcaaa gaatccttgt
      481 acttgaaccg tgtgcagatc taaagtcagt actcgagttg cccctgcttc ttcgatcaag
      541 ttagcgatca atttcgcagt gatcggttca tgaggtttcg ccgtgcggtc ttgcctagca
      601 tacccatagt aaggcaatac gacattgatc gttttcgcac ttgctcgctt cattgcatcg
      661 atcatgatca gtagttccat atagtagtca ttgacaggtg cattcgttga ttgaacgata
      721 tacacatgat cccctcgaat actttcttcg atattgatgg aaatctcgcc atcactaaat
      781 tgcttcacga cgcttttacc taattctgtt ccgaacactt tcgcgatttt ttcagctaat
      841 ggacggttgc cattcaaact aaaaatcttc aacgtgtcat cctgatactt attactcatg
      901 tagtcctccc caaaacttct ctattcttcg atagtttaac atatttttcc aagattttct
      961 gttaatttca gcaaattctt aaggaagtga aactgttccg ttactccaac tggcaggaat
     1021 gactttatga taatcgcccc agttgtcgtg tactttgtaa taacgttctc ctgtttcagt
     1081 ttcaaaataa gcgtacgctg tcacccaatg attgccatat gtactgccaa gtactttcaa
     1141 aataccaatc atcacaggtt tgccttcacg tatacgtttt gtagctcgct gccatgaacc
     1201 tagaagagtt gctcgtgcac gctcatgatt gccattcttc tttaaaaaac tagaaatccc
     1261 tgtactaacc tgaaaaggaa tcgtaggaag tcctagtggt tggatctggc tacgcaatgc
     1321 cttcgtcaaa accaacgaat cagccgtatt tttcttgcga atctcatttg gaagcatcca
     1381 ccccttacga tagtcttgat aataggcaag gagaactgct gccgcgtaag taccacaaag
     1441 atagcctgag ggggtagccc atttcttata cctcaaaaaa cgttcttctg gcaatcccac
     1501 ccagcaattt gcgagttcct ttcctgacct tttttcttcg atcaaaacgg gaatatcttc
     1561 ttttctgttg atccatctag ataactcgat tggctcttca ttgatcagct tccatttttt
     1621 acaagaaaga ccctcttgat aagtatattt tgctggagaa tattctaccg gatgtcctga
     1681 ttgatcgata atcacataac tttgatcttc aaaaaccaga gctaaaaaaa gcaatttact
     1741 ttttcttgac tgcagctgct gagtgctggc taatttttta atttttttat ctactttgaa
     1801 atataaaggt ttatccacta cttgattcag ttgggatcgc ctcctttttt tcattataag
     1861 tgaaggattg aaaaaaacaa atcagtttga ttgttcgaat tttcaatttt ttatgttcta
     1921 tttgttaatt ccttcatttt gtaagataat gggcacacga aacaaaaaag gagcgatcga
     1981 tgaatgaaca aagaacgatt gaaacaagat ttctatgaag cagtcaatga agaatggcaa
     2041 aaaacagcaa aaatccctgc agataaacca gctacaggcg gtttccaaga tttagttgac
     2101 gggatcgacc aacttttgat gggagaaaca gacgatctgc ttgcccatcc agaaaaagtg
     2161 tcttcagaaa aaatgaaaca tttccttgca tattatcaac tggcaaacga ttatgaccga
     2221 agaaatgagt tgcatgctca accacttcaa cctataatcg aaaaagttga atcattgcag
     2281 tctttcgacg aattgaatga gcaatttcca gaatggctgt tggatacatt accattgcca
     2341 tttaatctgg atgtagatgc agatatgaag aatgcgcaaa cgaacgcctt ctttgcttat
     2401 ccgccttctt tattccttcc agataagacg tattatgcat cagaccatcc caacggtgca
     2461 caactaatag gcgttttctt tgatatggct gttcagttat tagagcttgc cggcaaagaa
     2521 aaaagtcagg cagaagcaat cacggaacaa gcagtgcaat ttgataaatt gattgctcct
     2581 catgtaaaaa gtgcagaaga aaacgcagat tacagcaaaa tgtataatcc gcaatctttt
     2641 gatacattta ttcaatacac agataaactg gacttgaaaa aacttgtcac gggtttgatt
     2701 gggacaactc cagataaagt gatcgtaact gatccagtgt acttcgatca tttgaatgac
     2761 atcctgacag aagctcattt tccgttattc aaaagctgga tgatcattaa aacagtacgt
     2821 tctcttagtg gttatctatc agaagaattc cgccaagtca gcggaatcta ttctcgcgta
     2881 ttgtctggaa cagacgaagc aatgcctcag aaaaaagcag cgtattacct tgcctctgga
     2941 caattcgatc aagtagttgg cgattactac ggaaaaaaat attttggcga agctgctaaa
     3001 aaagacgtag aacaaatggt gaaaaaaatg atcgccgttt ataaaaaacg tctagaagaa
     3061 aatgactggc ttggggatgc aacaaaagaa aaagccgtca taaaattaaa taacttaggt
     3121 atccaagtag gctaccccga taagatccca gcattattcg accagttcaa aacagtacct
     3181 gcagatcagg gtggtacttt gctttcaaat actcttgttt tcagcaaact cacattaaaa
     3241 gatcgtttca gcaaatggaa caaaccagtg gatcgttcag aatgggaaat gagtgctgac
     3301 acggtcaatg cttattatca tccatttcgg aatgtgatcg tctttccggc agcgatcctt
     3361 caagcaccat tttacagtct agaacaatca agctctgcta attatggtgg aatcggagct
     3421 gttatcgctc atgagatttc ccatgcattt gataataacg gcgctttatt tgacgaatat
     3481 ggcaacttga acaactggtg gacagaagaa gatcttacac aatttcaaaa caaagcacaa
     3541 gcaatgatcg aagaattcga tggtctgcct tttgctgacg gaaaagtaaa cggaaaatta
     3601 accgtttcgg aaaatattgc tgatgcaggc ggtctaagct gtgcattgga agcagcaaaa
     3661 acggaatcag atctttctat cgaggaattt tttgtcaatt gggcaacgat ttggcgtaca
     3721 aaagcaaaaa aagaatacca acaactttta ctgcaaatcg atgtccatgc ccctgctaaa
     3781 ctccgagcaa acgtgcaacc aaaaaacctt gacgaattct atgaaacatt tggtatcacg
     3841 gaacaagacg ctatgtatct agcgccagaa aaaagagtgc gaatctggta agaggttgtg
     3901 aaaaagccgc ttagtttcaa gtagtaagaa aaattttgtg aaaatagctt tccacatttt
     3961 tgcataaatt tgacttacta c
//
LOCUS       DACEPW010000204         3942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEPW010000204 DACEPW010000000
VERSION     DACEPW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.209"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(225..1682)
                     /gene="ascB"
                     /locus_tag="IW819_002460"
     CDS             complement(225..1682)
                     /gene="ascB"
                     /locus_tag="IW819_002460"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1245546.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1679..3574)
                     /locus_tag="IW819_002461"
     CDS             complement(1679..3574)
                     /locus_tag="IW819_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1245547.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3632..>3942)
                     /locus_tag="IW819_002462"
     CDS             complement(3632..>3942)
                     /locus_tag="IW819_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1245548.1"
                     /translation="KKKTKLLDHYYKEENQIKIHKKIDSIQQLISPSSDLVMLPLNAE
                     TLLCCLIKSQVETKITEYQLEIPLIHQQRKIKRVFFVSFDYKRNDILVFFQAVSDLLG
                     "
BASE COUNT         1168 a          841 c          652 g         1281 t
ORIGIN      
        1 tcgttttttg agctaatgcc gatggtcagc tgattgtaca ccgtttattg agaggtcgtg
       61 agcctgccgt tgaactccga gtcacaagga gcaatttcaa aaaacagctt ctcatgtttt
      121 atgactatgg tgacttgtga tcgaggagtt ggcaggagaa tactgtttat tcgtaaagaa
      181 ggggctgtga caagactttt gtcacagtcc cttatttcct tttattatag gatttcgcca
      241 tttgattgga tgacttgtct gtaccaatca aatgattttt tctttaaccg tttctttgtg
      301 ccattccctt cgtcatcttg atcaacatag atatatccat aacgttttga catttgtgaa
      361 gtagaagcgc tgacgatatc aatgcagccc cacgacgtat aaccaaataa atctactcca
      421 tctttgatag cttccttcat ctgctcaata tgctgtttca aatagtcaat gcggtaatcg
      481 tcttgaacgg ttccatcttc ttctactttg tctatagctc cgagtccgtt ttcagctatg
      541 aacagtggtt tttgatacct atcatataat tgattcaaag caattctcaa acccttagga
      601 tcaatctgcc agccccattc acttgatgga agatacggat ttttaattcc tccagttatt
      661 aagtttcctt ggaccccttc ccaattttct ggatgagcac tgactgcact agacatgtag
      721 tagctaaaag aaacaaaatc aacagggtag gctgctaaga tttcttcatc acctacttct
      781 atttttaagt tgacaccttt ttcttcaaac attcgttgcg tatacgctgg ataatatcct
      841 cttgcttgaa catcactaaa gaacaaagaa gttctttgct gctcaattgt tttttgaata
      901 tcatccggat cacaagaata cggaacaaga agctgatagc tgatcataca accgacttgt
      961 gaacctttga taatttcatg acaagctttt gtagctaaag cacttgctat aaactgatgg
     1021 tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt tttcttcaat tactcctacg
     1081 ctaacaaaag ggtgatgttt tacacagttg atctcattga aagtcatcca atattttact
     1141 ttatttttat agcgtttgaa tactgtttca gcgaaacgaa cgtaaaactg aataagcttt
     1201 cgattaggcc atccaccata ttctgtaacc aaatataacg gcatttcata atgggaaaga
     1261 gaaacgactg gctcaatccc atattttaga cattcgtcaa acacttgatc ataaaaaaat
     1321 aatcccgctt cattgggtgt ttcttcatca ccatgaggaa aaattcttgt ccatgcgata
     1381 gacatccgat agcatttaaa acccatttca gcaaataaac ggatgtcttc tttaaagtga
     1441 tgatagaaat catttccgtg tcttttagga taataatact tgctgttggg atcctgcgcc
     1501 gcttttactt gctgatgcgt catcatatgc cattgatctg tccatttttc tttaggtaaa
     1561 tcgctgtcga atgtatacac atcagacaca gataatcctt tttcatctac atcccatgca
     1621 ccttcacatt gattggccgc tgtcgctccg ccccataaaa atccttctgg aaattttttc
     1681 atatcgatcc ccctaaactt gctagttttc ttgtttgatt atttttctta tgactagtaa
     1741 tgatattttt gaatctacta ctatttgtaa caataaccat tgtggtcgga tcatatccct
     1801 ctttgattaa ttcttgcaaa tctacttcca tcaataaatc gcctttacgt actttttttc
     1861 catcagatac tgcactttga aagtatttgc catttaaatt cactgtatca ataccgatat
     1921 ggatcagtat ctctgctcct tcttcactga ctaccccaat tgcatgtttc gttggaaaaa
     1981 ctgttgaaac ttccccgttt acaggactgt agatttttcc gtccgttgga atgatcgcaa
     2041 acccttctcc taaagatcca ctagcaaatg ttggatcgtt tacttcagat aaatcaatta
     2101 tttcacctgc tgcaggagta cttaacacta tatcctttga ggattcttct tcgatttcat
     2161 ccttgtagcc gaaaagaaaa gttagcatac aggaactgat catcgctaag aaataactag
     2221 caactagcca aacaaatcct tcaccaatat aaatcgggaa agacaaaata cttcttgtag
     2281 caactgccaa agcaccggct cctgcggcac cagctactcc accaaatata gctccacata
     2341 tacaagcaat gtaaaatggt cgtttatatt taagagtcac accataaaca gcaggttcag
     2401 tgattccaaa taatgctgtg ataaatgcag ataaagataa actttttact ttcttatttt
     2461 tcgttttcaa aaatacccca agtactgctc ctgcttgcgc agctacggca gtcatacatg
     2521 ctggacctag cgtatcacga ccgtagaaag aaagattgtt ggtcattact ggtacaagtc
     2581 cccaatgtaa cccaaaaatc actaacactt gccaaaatcc gccgataaca gcaccagata
     2641 aaacagggct aaagttataa acagctaaat atccagctgc aatcagctga cttgcataat
     2701 ctgcaattgg acctacaact aataaagtaa ttgggaccat cacgatgata caaatgaatg
     2761 gtgtcaataa atttcgaata ctttcgtgca agcttttgcg aatcttaggc tcaatataag
     2821 aaagaaccca tacagctaaa attgctggaa taaccgtact tgtatacctt gctagaatca
     2881 cgggcatccc tagaaatcgc aaggttatgc tatcactata tgcactgaca atcgtaggat
     2941 aaacgagagc tgctgcaata accatagcga tgaaacgatc tgtattgaac ttttttgcag
     3001 cagtatatgc caaaaatata ggcaagaaat agaaaaatgc atcagctgct gcattcaaaa
     3061 tgatataagt tcctgattca gtagttaata aattcaaact cgtacataag gcgagtactc
     3121 ctttgatcaa accacttcct gccaagacgc ctaaagtcgg ggcaaaaaca gcggcgatca
     3181 tgtctataaa agagtttaaa atgccttttt tctcagttcc tctctttcca ctagatgcat
     3241 catcttctag tttcatttgt ttggataata cgtcatacac ttcatttact gcatttccta
     3301 ctacgacttg aaacattcct ccagcttcga ctacagtgat cacaccttct agttgctcta
     3361 ccttctcctt atctgccttc ttattatctt ttagggtaaa gcgtaacctt gttgcacaat
     3421 ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg gacaatatct ttcgctaatt
     3481 gtgaatagtc catttttact tcccccttgt taattaatgc cgtagcaatc cttcttacaa
     3541 gataagtata gttcttagaa aacggtttct ccattcaatt agtacccctt ataatagcga
     3601 acgaaactgt acaatagtgt gtatggttta cttagccgag caaatcactc actgcttgaa
     3661 agaaaacaag tatatcgttt ctcttatagt caaaggagac aaaaaaaaca cgcttgatct
     3721 tcctttgctg atgtataagt ggtatctcaa gctgatactc tgtgattttt gtttctactt
     3781 ggcttttgat aagacagcac aacaaagtct cggcatttaa cggaagcata actaggtcag
     3841 aactaggaga gattagttgc tgaatggaat ctatcttttt atggattttt atttgatttt
     3901 cttctttgta gtaatgatca agcagttttg ttttcttttt tt
//
LOCUS       DACEPW010000205         3925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEPW010000205 DACEPW010000000
VERSION     DACEPW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.210"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(573..1130)
                     /locus_tag="IW819_002463"
     CDS             complement(573..1130)
                     /locus_tag="IW819_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245549.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IW819_002464"
     CDS             complement(1309..1920)
                     /locus_tag="IW819_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245550.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IW819_002465"
     CDS             complement(2033..2290)
                     /locus_tag="IW819_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245551.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IW819_002466"
     CDS             complement(2887..3165)
                     /locus_tag="IW819_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245552.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IW819_002467"
     CDS             complement(3199..3450)
                     /locus_tag="IW819_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245553.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DACEPW010000206         3873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEPW010000206 DACEPW010000000
VERSION     DACEPW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.211"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..120
                     /locus_tag="IW819_002468"
     CDS             1..120
                     /locus_tag="IW819_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1245554.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            125..346
                     /locus_tag="IW819_002469"
     CDS             125..346
                     /locus_tag="IW819_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245555.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            359..859
                     /locus_tag="IW819_002470"
     CDS             359..859
                     /locus_tag="IW819_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1245556.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            956..2803
                     /locus_tag="IW819_002471"
     CDS             956..2803
                     /locus_tag="IW819_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ1245557.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2806..3702
                     /locus_tag="IW819_002472"
     CDS             2806..3702
                     /locus_tag="IW819_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1245558.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3752..>3873
                     /locus_tag="IW819_002473"
     CDS             3752..>3873
                     /locus_tag="IW819_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1245559.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLS"
BASE COUNT         1358 a          501 c          826 g         1188 t
ORIGIN      
        1 atggaaattg tgaaagatat tttattggtg tttggtggca gtatgcttgg tgttactgtg
       61 atgtgcttga tgcatgcaag tgcagcggcg gaccgagcaa tggaaaagaa aggaaagtga
      121 tgaaatgaat ttcggtcaaa atttgtatca atggttttta accaatgctc aatctttagt
      181 cttactagcg attgtggtca ttggtttgtt tttaggattt aaacgcgagt tttccaaact
      241 gattggcttc ttggtgattg ctttgattgc ggtaggtctg gtctttaatg cttctggtgt
      301 gaaggatgtc ttactcaatt tgtttaaccg aattgtcggt gcctgagggg gaatcacaat
      361 ggaacagata cgtgtttata ttgcgaacct tggaaaatac aatgaaggtg agttggtggg
      421 ggcatggttt accccacccg tcgattttga tgaagtgaaa gaacggattg gattaaatga
      481 cgaatatgaa gagtacgcaa ttcatgatta tgaactgcct tttgagattg atgagtatac
      541 accaattgag gaaattaatc ggttgtgtgg tttagcggaa gagcttaaag gaacgccaat
      601 tggtgaggtc gcttcagaga ttcaacatgc cttcttcaat tctttcgagg aaatggtaga
      661 gcatgtggat gatatcatct attatcccga ttgtaatgac atgagtgatg tagcgtatta
      721 cttgattgta gaagctggtg atttaggcga agtaccagaa cacttgaaga cgtatattga
      781 ttttgatgcg tgtggtcgag acttggagat tggcgggaat tatttggtta cgagtcgagg
      841 ggtttttgaa ctaatgtaaa tcatttgtta gaattgacca gaaatttcta tcgtactttt
      901 acttttctat ttgatatact gaaatagaaa atatttgttg ataggggtgt tactaatggt
      961 tggcaataag tggtacaaat gtgatcttca tattcattca actgcgagtc agtgctttca
     1021 ggataaaaag gttactgcta aggaatgggt agaagaatgt ttaaacaaag gactcgattg
     1081 tgttgcatta actgatcaca atacagggga aaatattgat gaatataaga aagtggctat
     1141 cgaaaatggc ttagattttt ttcctggagt ggaattaacg tgtggagaaa atggaactca
     1201 tttactagtc ctatttgata aaagtgcatg tacaaatgat atctctgatt tcttaaccaa
     1261 gatgggtata aaaagagagg attgggccag cgctgcagct tgctcaatga aatcagtttt
     1321 agaagttatt gattttgctg agcacgaggg cttgctcgtt attcctgcac atatagatga
     1381 gtttaatgga atttcttcag caaaatttgg aaatctagga aagatatttg aatcagaaaa
     1441 tatatatgca gtacagacgg ttcaaaaaga attttttgaa aatcgctcac aaatgattcc
     1501 ctcatctaag cgtgacacta tctatgatag tgtgaatgcc agatatgatg gtagagttgg
     1561 aaaagatact ttggaatctt ggtataaatc agttgttgag gcagaaaaaa ataatatgac
     1621 atttcttagc ttttctgata acccacactc gaaaggaaat tcaaaacatg gactttgggg
     1681 aataggcaca agatattcct atataaaaat gaaggacgaa cctgacttgg gatcacttcg
     1741 cgatgcttta atgctaggcg aaactcgagt tcattctgat ttttctaatt tctccattaa
     1801 tgaaaatgaa gttttattgg aaaaattaag cttttctggg accactctta gcacaaaaga
     1861 agttgtagtc gaattcagcg ataatttaac ttcaattatt ggaggaagag gaacaggcaa
     1921 atcttgtatt acaagattct tggtctatgt ccttggaaag gaagcagaac ttgatcaatt
     1981 ttctgaaata caaagtgatt atcagaactt tgcacaaatt gaacataatg gctctggaat
     2041 atttttgaag gatacaattg tgaaactgaa tattttctat aaggggacaa aatatcaaat
     2101 aataagaaca caggatagac attctattta tgaatttaca caagagaatg gtctggttga
     2161 aaaagaaaca gaacggttga gaatgatttc cgataaggtg agtatatact ctcaaaaaca
     2221 aatttatgaa atttcaaaaa atcaaactag tatactagaa ttactagatg gatataactc
     2281 tgatttgatt tcagagtata aaaatgagat tgaaacatgt gtaaacgaaa taagaaaatt
     2341 aaattgggat attgtttctg tgaaaaaaga aatacaagat aaggcgaagg tagagcttga
     2401 aattgaagat ttaaaaaaac aagtagaaaa gctatctcat aagagttata aagatgttta
     2461 tgatgagtat tcaaaagaga cagatattta tagggaactg aaacgtgatg tggaggcact
     2521 taaggaaatt cccaaaaatc taactgatag tgttgacaaa attgactttg gcaatggtat
     2581 aaaagttaca gacgaaattg atgaacaccg cggagaattg attaagaatt tagtgaccaa
     2641 tcgggctaag attcaaacaa taattaatga aatgtcagat gaaattgata gttatagagc
     2701 agagctgaat aaatcagatt ggaaagttaa ctataatgag gtttcaaaga ggtatcaaag
     2761 aggtatcaaa aacttagtga agagttatct caagatgaat taaaaatgtt acaaaacctt
     2821 gattctttat atcaatcttt atcaaaaact gaagagcgat tggaagaaat tattcaacaa
     2881 gaaataaagc ttaaagatta ccaagttgag gttgactcag caatgtcaag aatgaataaa
     2941 tcatatcaaa atttctattc tgagagaagt agttcctcgc gcgaaatttt ttctgatatt
     3001 acaggaatag aggctactat tcaaccaaat agggatttca ataattatgt taaagagctc
     3061 agagcttgta ttaataagga ggacactttt gacagtgagt ttggatctct tattgataag
     3121 ctgacaaaaa attccattac taatcaagat atatataatg acatacttga tataagagtt
     3181 agtggggaaa gtagtatttt ttcagataaa aaattaatca attctataag aaaattgaat
     3241 gatgcacaag ttcttgaatt acaaaccctt aagccaaatg atttagtagt tatccgactt
     3301 gaactaaatg gaaaaaaagt tagtttatcg aatgctagtg ctggtcaaaa gacatctgca
     3361 attctttcta tgattttggc gtatggaaat tcaccactca tattggatca gcctgaagat
     3421 gatttggata gtcaattaat taataactta atcgtaagaa gtatcattac aaaaaaggaa
     3481 aacagacaaa ttttaattgt aactcataat gcaaatatcc cagttaatgg tgattcagaa
     3541 tggttgatta gcatgggaga ttcaaaagaa atatcggctg atcagtgcgg gtcggtagat
     3601 gaacccaata tcaagccaag aatatgtact gtgatggaag gtggcgagga tgcttttaca
     3661 aatagagcga aacgctatgg ttttaaacaa attgtagaat aacactagag ggtgagacat
     3721 attattatgt ccgccctttt taggaggaaa aatgaaaaaa ataaaaagct ataccagtat
     3781 ctgggcagtt gaaaaagtca tttacgcaat caatgatttt cagttgcctt ttcctgtgac
     3841 ttttaaccaa atggcttggt tcgttttatc ttt
//
LOCUS       DACEPW010000207         3849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEPW010000207 DACEPW010000000
VERSION     DACEPW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.212"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            394..1083
                     /locus_tag="IW819_002474"
     CDS             394..1083
                     /locus_tag="IW819_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1245560.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1070..2278
                     /locus_tag="IW819_002475"
     CDS             1070..2278
                     /locus_tag="IW819_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1245561.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2572..3843
                     /gene="serS"
                     /locus_tag="IW819_002476"
     CDS             2572..3843
                     /gene="serS"
                     /locus_tag="IW819_002476"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1245562.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1263 a          666 c          774 g         1146 t
ORIGIN      
        1 caagtggttc ttttttaacg actcagttat cttatcatgt catttccaac atgtcaacaa
       61 cttatttttt attatttaat aagttattgg tgttcttttg aacaaataat aatatatcaa
      121 catcacatgt gaaagtcaat aagaaaatgc ttatttatca acatttctag cttacatgat
      181 cattggttag ttctggtcct ttttcgttcg tctttataac tccgagagac gtaaaatcct
      241 tttgttttca gtttaatgtt catgttcttg tcttattcac accaactaga tgtctcacct
      301 tctaaaattt tccttaacaa agtgctgctt ttatctattt ctgctttttt ttgctagaat
      361 aatagtaaca atatgactct ggaggattta tttatgaaaa ttttgattgc tgacgatgat
      421 aaagaaatcg ttgaattatt aagtatctat gttcacaatg aggggtatga agcagtaaag
      481 gcttatgatg ggaaagaagc attatctaaa atcagaacaa tccctgatat tgaattactt
      541 attttagata tcatgatgcc tgagatggat gggatgcaag tagtgaaaga gctacgaaaa
      601 gaatcccaaa taccaatcat tatgctgaca gcaaaaacta ctgatatgga taagatcaaa
      661 ggactggtcg ctggtgcaga tgattatgtg accaagccat ttaatccgtt agaagtaatg
      721 gctagagtaa agtctttatt acggcgtaca aaaatgcaga ttgccccaga tcagcctgat
      781 gtattagaag taaatgcatt aatcatcaat aaagactctc atgaagttaa aacaatcgat
      841 gggaaagaaa tccagttaac agctttggaa ttcggtatcc tctacttgct tgctagtcat
      901 cctaatcgag tatttagcgc ggatgaaatt tttgagcgtg tttggaaaca agaaagtatt
      961 gtctctgcta aaactgtcat ggttcacgtt agtcatttaa gagataagat tgaagaagca
     1021 actggtggtg aaaaagtcat tcaaaccgtt tggggagtgg gctataaaat tgacgcaaga
     1081 tagtaaacga gcaactgaaa aacgccgtcg gatcaatttg acttcaaaag aaatcagcga
     1141 attgttagcg gaaggaatca tcacgattat ccttttgctt ctattaaatg tttctattct
     1201 ggttgttatc agttcggtga tcaacagttc accttctttg acgaatgcga tttgggattc
     1261 aaagaatatt tttgcagaac gattgaacac tgatttgttc tggaacggcc ggaattttat
     1321 tattcccttt ttctttctat tagatattgg tgttttgtat tggcgcttga ttcgaagata
     1381 tcgccaaatg caattgcggc atatcatcag cgaacttcac tacatcgcaa acggaaatta
     1441 tgatcatcgt atcccttttg aactgagtgg tgacttaagc cgagtggtaa caagtatcaa
     1501 tggattggtg gatagtaccg tagcagcgat tgaagacgaa cgtaaaatcg aaaaatcaaa
     1561 agatgaattg atcacgaatg tcagtcatga tatccgtacc ccactcacgt ccatcattgg
     1621 atacctagga ctgatagaag atggccagta tcactcagaa gaagatcttt taaagtatac
     1681 gcatactgct tatatcaaag caaagcagat gaaatcttta gtggacgact tatttgaata
     1741 cactaaggta cgccagcctg ctgtcccggt gaatttttct gcctttgaca tgatccagtt
     1801 gatcgaacag ttagcagctg attttgagtt ggaagcttct aaaaaaaata tccaaatact
     1861 tgttcaatca aaagtagact cccttatcat ggatggcgat accgaaaaac ttgtacgtgt
     1921 cttcaataat cttttgacga atgcgttgaa atacggaaaa ggagcaacga agatcgtaat
     1981 cgaagtagaa cgtatcggtt cagaagttgt tgcaacagtc aaaaataacg gagcaatgat
     2041 cccacaacag gcaatcgata atctattcga ccgtttttat cgtgtagaag aatctcgttc
     2101 acaagcaact ggaggaactg gacttggttt agcaatcgct caaagcattg tggctttgca
     2161 cggcggatac atttatgcaa aatcagataa gcaatggact tctttcatca tgcaccttcc
     2221 aatcaagaaa aatgaaaaat tacctattga tacacaggaa gttattgacg aaagctgaaa
     2281 tattcgctat tcttattaca attattagaa taaatgaaaa acacagaacc cagtacactt
     2341 tattcttttt cagagagctg atggcaggtg taaatcagca aaggataatt tggaagttcc
     2401 agttcgttta cgtgcccttt cgaaataaga aaggtcggtt caacagccgt tatgtgttca
     2461 acaagtgcag ctgacgctga acttgggtgg taccgcgaat ttcagactga ttcgtcccaa
     2521 gaataatggg atggatcagt ctttttttat tttcaaaaag gaggaaatca tatgttagat
     2581 gtaaaaatga tgcgacaaaa tattgaagaa gtacaagaaa aattgatgac tcggggcgta
     2641 aaaaaagaaa cattagctga attcatggaa ctagatgaaa atcgtcgtaa gctactagtc
     2701 aaaagtgaag aattaaaaaa atatcgaaat gatgtttctg ctgagatcgc ccaattgaag
     2761 cgtgcaaaag aagatgcttc tgctaaaatc gctgaaatga aagaagtcgg tggcaacatc
     2821 aaagcattgg atactgaaat tgctgaagtg gatgaaaaat tgaaacaaat tgcaactact
     2881 ttgccaaacc ttccccatga ttctgtgcca gttggaacag atgaagagga caatgtagaa
     2941 atccgtcgtt ggagcgaacc acgtacattt gcttttgaac caaaaccaca ctgggaaatt
     3001 gcagaaaatc ttgatatttt agattttgaa cgtggcgcaa aagtagccgg tagccgtttt
     3061 gtctattaca aaggattagg tgctcgatta gaacgtgctg tctacaactt catgcttgat
     3121 cagcatattt acgaacacgg ctatactgaa atgatcactc cttatatcgt caacagtaaa
     3181 gcgatgtttg gtacgggaca atttccaaaa ttcaaagaag atgttttcca attggagaat
     3241 actgatctaa cattaatccc aacagctgaa gtaccattaa ctaactatta taatgatgaa
     3301 attttagatg gtgcagattt acctatttat ttcactgcac taagccctgc ttttcgttct
     3361 gaagccggaa gtgctggtcg ggatacacga ggactgattc gtcttcacca attcaataaa
     3421 gtagaaatgg tcaaattttc ggatgtagag cattcttacg aagaattgga aaaaatgaca
     3481 gctgatgcag aagatatctt gaaaaaattg catttgcctt atcgtgtgat ggccttatca
     3541 actggagaca tgggcttttc tgcagctaaa acttatgact tagaagtatg gatacctgca
     3601 caagatactt atcgtgaaat cagttcttgt tctaactgtg aagatttcca agcacgccgc
     3661 gcaatgatcc gttatcgcga taatgatgga aaaatccact atactcatac tctgaatggt
     3721 tcaggacttg cagttggccg tacagtagct gccattttgg aaaactacca aaatgaagat
     3781 ggttctgtca cagttccaga agcgctcgtt ccttatatgg ggaatctgaa agttattaaa
     3841 taaagaggc
//
LOCUS       DACEPW010000208         3816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEPW010000208 DACEPW010000000
VERSION     DACEPW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.213"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(39..305)
                     /locus_tag="IW819_002477"
     CDS             complement(39..305)
                     /locus_tag="IW819_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245563.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(316..510)
                     /locus_tag="IW819_002478"
     CDS             complement(316..510)
                     /locus_tag="IW819_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245564.1"
                     /translation="MPDANIGREKVLQYLEENNIQKIDLAVLYGMPKQDIGNYLEGKL
                     VDTPKAKQLLVKIISDFKIR"
     gene            815..1234
                     /locus_tag="IW819_002479"
     CDS             815..1234
                     /locus_tag="IW819_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1245565.1"
                     /translation="MTVFDRIKELADKQGISVSKVATDLGFSENLFYQWKKASPKSDR
                     LEKVADYFNVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKKD
                     NEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            1251..1673
                     /locus_tag="IW819_002480"
     CDS             1251..1673
                     /locus_tag="IW819_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1245566.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEPGKMVTCSHELGHCILHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            1702..1902
                     /locus_tag="IW819_002481"
     CDS             1702..1902
                     /locus_tag="IW819_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245567.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            1988..2428
                     /locus_tag="IW819_002482"
     CDS             1988..2428
                     /locus_tag="IW819_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245568.1"
                     /translation="MKFIKFGIIGLLVVTVMGGCGSSTAETKESKNYSETNSTIKEVD
                     SDQTAEFATIAEKHIKEIYSIDNLKMDLKSIKVTQFPDDKNAETGEEYKNVYNGTGDF
                     TWQDKKYNFSLIYSKKDATNYTVLYLYSNLDPNKGVDTPLESDQ"
     gene            2566..3771
                     /locus_tag="IW819_002483"
     CDS             2566..3771
                     /locus_tag="IW819_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1245569.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSVPRGEALDIEDKNIEFYTKEELIEFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQSENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
BASE COUNT         1443 a          600 c          632 g         1141 t
ORIGIN      
        1 actaatgttc gattaaactt tcccatgttt ttcctccttt aaattttgta tttagccatc
       61 agttcatcga tgtccttttt gtcatatctc aaaaagccat ctatacgaat ttcttttaaa
      121 ccgtgagcta ttaacttctc aaagccttga tcgtttacac ctcccacata ttttctagct
      181 tcgatctttt tcaaatatcg tgtttctgac aaattctttt ttgaaagttg actaagtgcg
      241 tcctttacaa cttcaacaat tgcgattctc aatcgagtca gaaactcttc gcttagaata
      301 ttcatgtcta gcctcctatc gaattttgaa atctgaaata atttttacaa gcaattgttt
      361 tgcttttggt gtatcaacca acttaccttc caaataattt ccgatgtctt gtttgggcat
      421 accgtataaa acagctagat caattttttg aatattattt tcttctagat attgaagaac
      481 cttttctctg ccaatatttg catcaggcat tatatttctc cacctttcta tatgaattaa
      541 gtaagaaaaa tagtaagaaa tttcatcttt ctattgactt ttttatagaa aactatagaa
      601 taaatacata gttaaataag cgtagtaaaa ccctataaaa ataactttct aagtttggcg
      661 acctcgaatt gtttattatt gacaggcgtt tttgctttgg cttgtttctt actatttatc
      721 ttacgaggac tatattatag caaactataa atttagtcaa ggtttttcta tagttttctg
      781 taattttagt tctatggtga aaggaatcgt tgatatgaca gtctttgaca gaataaaaga
      841 attggctgac aagcaaggca tcagtgtttc taaagtagca actgatttag gttttagtga
      901 aaatcttttt taccaatgga aaaaagcaag cccaaaatct gataggttag aaaaagtagc
      961 agattatttc aacgtatctg ttgattactt actaggacgt actgagaaaa aaagatacta
     1021 tgatttaaca gaaaaagacg agcgtgatat ccaaaaagaa ttgcagtcat taatcgacga
     1081 tttatctaat gctgatggaa tggctttttc gaagaaagat aatgaaatga gtgaagcgac
     1141 acgtgaagct ctcattattt ctttagagaa tgctttgcga atagctaaaa tagaagctaa
     1201 gaaaaaatac acacctaaaa aatatcgcga ttaaaaggag tacgttgttt atgtatttac
     1261 ctcaaattga taacaaaatt aataagctgg taaaactata tcaaacacgc aatcctttta
     1321 gaattgcacg agaattaaag atactagttt tagaagaaga tttgggagaa atttatggtt
     1381 actatagtaa attcaaacag ataaaaatga tccacataaa ttctagttta gatgagccag
     1441 gaaaaatggt gacttgtagt catgaattag gacactgtat tttacatcca aatgaaaata
     1501 cacctatgtt gtctaaaaaa acgctagtat ctgaattaaa aattgaaaaa gaagcaaact
     1561 atttcgcaac tcatctgact attgatccaa ctttagacgg ctttgatcat atgacaaaat
     1621 atgaaaagct cgtctgcttt ggattaccag atgaattcga tcgtttcttg tgaattataa
     1681 tacaacgtaa ggaggatata gatgaaaata ggaatgagaa aaccaagtat aaaaaaatcc
     1741 ataagcgcta gaaccactgg aaaagcaaaa agaaaagtaa aaaaagcagt tattcctggt
     1801 tatggaaaga aaggctctgg atggattaaa gacccaaaaa aagctgcata caataaagtt
     1861 tataaaaaga ctacgtttag cttttgggac ttatttaaat aaaaaaagcc cgtgcagcaa
     1921 cacggactcg aaaccttatt tctaagattc cccaataaaa taatatcata gagataagga
     1981 gtagaaaatg aaatttatta aatttggaat tattggatta ctagtagtga cagttatggg
     2041 aggatgtgga tcatcaacag ctgaaacaaa ggaatcaaaa aattatagtg agacaaacag
     2101 tactatcaaa gaagtagact ctgatcaaac cgctgagttt gcaactattg ccgagaaaca
     2161 cattaaagaa atatattcaa tagataattt gaagatggat ttaaaatcta tcaaagttac
     2221 tcaatttcct gacgataaaa atgcagaaac tggtgaagag tacaaaaatg tctacaatgg
     2281 tactggagat tttacttggc aagacaaaaa atacaacttt tctttgattt attcgaaaaa
     2341 agatgcaact aattatactg tcctatatct atatagcaat ctagacccta ataaaggtgt
     2401 agatacacca ttagaaagcg atcaataaaa aagcacattt gccgcccgac caagaacgca
     2461 aatgtgcaaa gagctacggt aaagtaggtc tatttgttgt accctatttt accatctcga
     2521 aatccgctgt acaaccgaac aaacgtacga aaggaaagat aaaagatggc aaaaaaagaa
     2581 attgataaac gaattaaaaa gcacttaaca aaaaaaggag aagaaaaata tcagtttagt
     2641 ctgtacttag gagttgatcc gctaacagga aaaaaaagaa aaacaacgag acgaggtttt
     2701 agtacgccat tagccgctga gagagcttta aaaagattgg aggcagatat acaggaaaaa
     2761 ggactgcaag cttctacagc tcaaaagagc aagaaattcg aatatgttta cggcttatgg
     2821 tttgaaaatt ataaaaagac agttaaagaa agcacttggt cctccaccaa acaaattttt
     2881 gacacacaca tcttaaaagt gtttggagat aagttcattg acaaaataga cgtttttttc
     2941 tgtcaagaag cagtaaacac ttggtccgat agccatccta aaatttttaa gaaaataaaa
     3001 aattacacat ctaatgtttt tgattacgct gcttctttac aaattataac tagcaatcct
     3061 atgaaattag tttctgttcc acgcggagaa gctctagaca tcgaagataa aaatatcgaa
     3121 ttttatacta aagaagaatt aatcgaattt ttagaagcaa taagaaacga tgatgatgaa
     3181 agatatttat tcttttccct attggctttt acaggtataa gaaaaggaga agcttttgca
     3241 ctgacctggt cggatattga ttttaagagc aaaacactca atattaataa aactgtaaca
     3301 agaggttatc aagggagatt gatcgtaaat actcctaaat caaaatctgg aaaaagaaaa
     3361 atttatctgg acaatgattt gatcagttta ttgagaaagt attatacaaa aaacaaaacg
     3421 atcgttacaa tccaaagcga aaacttaatt tttcaccatg atggtcttct ctataaccct
     3481 acagtttcac gttcgtggct taatgtcata tataagcatc atccagaatt gactaaaaga
     3541 atcactactc atggttttcg acatactcat gcctctctcc tttttgaatc aggagcttct
     3601 ttaaaagatg ttcaggaacg tttagggcat gcagacattc aaactacttc aaatatttac
     3661 actcacgtaa ctgaaactca aaacaaaaaa gtcatcaata attttgtagc atttatgaag
     3721 aacagtactc agggggagtc aaaaggggag tcaaaaaata ttttgggcta aacatacaac
     3781 aaaaaagaca cgatcgaaat cgtgtctaat cgttga
//
LOCUS       DACEPW010000209         3753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEPW010000209 DACEPW010000000
VERSION     DACEPW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.214"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..145)
                     /locus_tag="IW819_002484"
     CDS             complement(<1..145)
                     /locus_tag="IW819_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1245570.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFK"
     gene            260..1972
                     /locus_tag="IW819_002485"
     CDS             260..1972
                     /locus_tag="IW819_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245571.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            1975..3744
                     /locus_tag="IW819_002486"
     CDS             1975..3744
                     /locus_tag="IW819_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245572.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
BASE COUNT         1184 a          646 c          817 g         1106 t
ORIGIN      
        1 ttttgaaata ccaatataaa gcaggtgctt gaacatgaat tctgtttgcg atgtttcgca
       61 tggaaagctg cgaaagagct tgtgtttcag ccaggatatc aaaggcggcc tgaatgatga
      121 ttgcttgaga aagctttatt tccattttat caacctcgtg tattgcatta tctttttaaa
      181 catggtaaat tgttagtcgg tctaatttta cagtgttaaa ttagtcctgt caatttaaac
      241 aaaggggtgt aatcaacgaa tgaaattaat cttacgacat gcgaaaaaat ataaagtagc
      301 tgtttttatt tccttgctgt ccgtagcagt catggttgca gcagcattat ggcaacctaa
      361 gttactacag caagtattgg aagctattat tacagaagat aatgatgaaa tgaaaacaat
      421 tggtatttac ctgatcagtc tggctttgct aggattagcc gcaggtgtaa cgaacacgat
      481 tttctctgca aaagtagctc aaggcgtaag tgcagatatc cgagaagaag catttcgaaa
      541 gatccagaca ttttcatttg gaaatatcga acagttctct gctggaaatc ttgttgttcg
      601 tctaacaaat gatatcacgc aaatccaaaa tttagtgatg atttctttgc aatcactttt
      661 tcgaattcca ttcttattta ttggtgcctt cattttagct atgatcacga tgccgcaatt
      721 atggtggatc atcgtagcgc ttatcgttac ggtatttctg attacggcac tttcatttac
      781 aaggatgggc aagcacttca tgatcattca aaaactgatt gatcgtgtga atagcattgc
      841 aaaagaaaac ttactaggga ttcgtgtagt gaaatctttt gtacaagaag aaaaccaatt
      901 gaagtcattc agcaaagtaa gtgaggactt aacaaaacac aacatcatag tcggtacatt
      961 attttctgtg atgatcccgt ccttcatgct ggctgccaat ttggctgtgg ttggggcgat
     1021 cttttttgtg agcgatcttg ttaaagatga tcctacgtta attggtggaa ttgcttcatt
     1081 catgaactac ttgatgcaga ttatgatggc aatcattatt ggcggaatga tgatgatgat
     1141 gacttcaaga gcagccgtat cgctgaagcg gattgcggaa attttagatg ctgaaccaga
     1201 tcttacttat ttagacgttc cagaacaaga gttgacgggt tctgtcactt ttgaccatgt
     1261 ttcttttcgt tatcctggtg aggatacaga tacgcttaaa gatatcagct tttctatcaa
     1321 acctggagaa atggttggta tcgtcggggc gactggtgcc gggaagtcaa cactagcaca
     1381 attgatccct cgtctatttg atccgacaga aggcaaagtt gaagtaggtg gtgtagatct
     1441 tcgtcaagta aatgaaaaga gtttaagaca aacggtttcc tttgttttgc aaaaagcaat
     1501 tttattctca ggaacgattg ctcaaaactt gcgccaaggg aaaaagaatg caacagaaaa
     1561 agagatggaa catgcttctt ctattgctca agcaaaagaa ttcatcgaaa agttatctga
     1621 tcgttacgaa gcaccaatag aagaacgtag cagtaacttt tctggtggtc aaaagcaacg
     1681 tttatccatt actcgtggcg ttatcggtaa tccaaagatc ttgatcctag atgacagtac
     1741 gagtgcactg gatgctcgct ctgaaaagct ggtccgagaa gcactcgatc gagatttgaa
     1801 gggaacaacg acgattgtca tcgcacaaaa aattgcttct gttgtcaaag cagaccgcat
     1861 cttagtattg gatgaaggca gattagtcgg cgaaggaacc catgaggaat tggtagctgg
     1921 aaaccgtgtc tatcaggaaa tctttgaaac tcaaaaagga acggaggagt aagtatgact
     1981 gatctaataa aagcaagtaa atttttctat cactatttga agcggtacaa actctccttt
     2041 ttctttatct ttatcacagt gatcattgct acctatctac aagtaaaggc acctcaatac
     2101 atcggggaag cttttcaaga attagctaat tacgtaggag gcttgatgca aggcgtagac
     2161 gataaaagca aatttgtttc tgtaatatgg aaactcttgc tgttttatgt actagctagt
     2221 gcagccaact ttatctatag tattttgttc acacaagtag tagggaagtc aacgaaccga
     2281 atgcgtatcg gtctattcaa caaattggaa aaattgacga tccgtttctt tgattctcat
     2341 caagacggtg aaattttaag tcgttttacc agtgacttag ataatattca aaatagtttg
     2401 aaccaagctt tgcttcaagt catcacgaat gctgttttac ttgtcgggat cttgatcatg
     2461 atgttccgcc aaaatgtaga gttagcttgg gctacaattg cttccactcc agtagccgtt
     2521 ttgatagctg tcttgattat ccgcaaagca cggaaatatg tggatcttca gcaagatgaa
     2581 gtggggaaac taaatggtta tatggatgaa aaaatcagcg gacaacgtgt catcatcaca
     2641 aatggcttac aagaagaaac gattgaagga ttcttagagc ataatcaaag tgttcgcgaa
     2701 gcaaccttca aaggacaagc ttattctggt ctactatttc cgatgatgca aggaatgtca
     2761 ctggtcaata cagcgatagt tatctttttc ggcggctggt tagcattaaa tggtgatttg
     2821 gaacgttcgg tggcattagg cttagttgtg acattcgtcc agtattcaca acaatattac
     2881 caaccactga tgcagatttc atctggatac agcatgatcc aattagcgat tacaggggca
     2941 agacgattga atgaaatatt tgatgaaaaa gatgaagtca aaccagaaaa ctgtaaagca
     3001 tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag attttggtta ttctccagaa
     3061 aaactagtct tgaaagatgt ctcgatcaat gtggacaaag gagaaatggt tgctcttgtt
     3121 ggtccaacag gatcggggaa aacgacgatc atgaatctgc taaaccgttt ttacgatgtc
     3181 aatgatggtt cagtagtcat cgatggaata gatatccgag attatgatct tgatgcatta
     3241 cgtagacatg taggaatcgt tttgcaagaa tctgtattgt tctccggaac gatcaaagac
     3301 aatatcacat tcggcaaacc ggacgcttca gatgaagaag tcatcaatgc agcaaaacaa
     3361 gcgaatattc atgacttcat catgacgtta gaaaaaggtt atgatacaga gatatccgaa
     3421 gaaaataact tattcagtac aggtcaaaag cagttgatca gtattgctcg aacgatcatc
     3481 acaaatccat ctttattgat tttggatgaa gcaacaagta atgtagacac ggtaacagaa
     3541 gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa gaaccagctt tgtgattgct
     3601 caccggctga aaacaatctt gaatgcagat agaatcgttg tcttgcgtga tggggaagtc
     3661 attgaagaag gcagtcatca cgagttatta gcagaaaatg gattttatgc ggaactttat
     3721 cataatcaat ttgtatttga ataaagaggt tgt
//
LOCUS       DACEPW010000210         3723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEPW010000210 DACEPW010000000
VERSION     DACEPW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.215"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..502
                     /locus_tag="IW819_002487"
     CDS             <1..502
                     /locus_tag="IW819_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1245573.1"
                     /translation="TFEEYTTMELLSVSMLIVISTFALDFIHSNREIFKIVDLDEAWS
                     FLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLKFAFRSTDIHEI
                     KKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPP
                     VQERME"
     gene            507..2630
                     /locus_tag="IW819_002488"
     CDS             507..2630
                     /locus_tag="IW819_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1245574.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            2627..3631
                     /locus_tag="IW819_002489"
     CDS             2627..3631
                     /locus_tag="IW819_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1245575.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
BASE COUNT         1180 a          633 c          871 g         1039 t
ORIGIN      
        1 cacttttgaa gaatatacca ccatggaatt gcttagtgtt tccatgctaa tcgtcatttc
       61 cacttttgcc ttggacttta tccatagtaa tcgagaaatc ttcaaaattg tagacttaga
      121 tgaggcgtgg agttttcttc aagtagcgca agggaaaaca ttatcgaata agctcgttcg
      181 tgctgggcgg gctatgaatg ccggggttta ctttgtcacg caaaatagtg atgatttact
      241 ggatgaacgc ttgaagaata atatcgggtt gaagtttgcg tttcgcagta ccgatattca
      301 tgaaatcaag aaaaccttgg aattctttgg cttggatcga gaagatgaaa gcaatcaaaa
      361 acgcctacga gatttggaaa atggccaatg cttgatgcag gatttgtatg gtcgggtagg
      421 cgtggttcag gtacatccag tctttgaaga actcttccat gcctttgata cccgtccacc
      481 tgttcaagag agaatggagt gaggggatga aaaagaaact ttttcggatt gtgggaatcg
      541 tttttattag catactcact ctgatagttc tattgagttt agttggtacg gtagccgagg
      601 cgacagggct agttgatgat acagttgaag caggaaatct atattccaaa tattcactga
      661 acaactatca actagatttc tttgtggata gctcttggga ttggttgcct tggaattggg
      721 gtgatggctt agggaaaagc gtgatgtatg gtctttatgc cattactaat ttcatttgga
      781 cggtgagttt gtatttgtcg aacgccacgg gttatgtggt tcaagaggct tacaaattag
      841 atttcatttc ggatacggct gaaagtattg gaaagaatat ccaaacattg gctggtatta
      901 ctgaaaatgg actccagacc tccggatttt attttgggtt tctattattg atgattttag
      961 ctctgggggt gtatgtggcg tataccggat tattaaaacg agaaaccacg aaagcagttc
     1021 gagccgtgat taattttgtg atgatttttc tactatcggg ttcgttcatt gcttatgcgc
     1081 ctacctatat cacaaaaatc aatgacttta gctcagatgt tagtgaagcg gcccttaccc
     1141 ttggaaccga gatcgttgta cccaattcag aaagccaagg aaaagatagt gtggatttga
     1201 ttcgggatag tttgttttcg attcaagtcc aacaaccttg gttactactg caatttgatg
     1261 attccaacat agaagaaatt ggtgaagacc gggtcaacaa gattctatct gtgagtccag
     1321 atgaaaacaa aggcaaagat cgggaagaag cggtcaaggc agagattgaa gacaatgaca
     1381 atgcgaatct cagtattacc aagaccatga accgtttagg gaccgtggtc tttttggtat
     1441 tgttcaatat tggcatttcc ttctttgttt ttcttctaac agggattatg ttgttctcgc
     1501 aaattctctt cattatcttt gcgatgtttc tgccaattag tttcttattg tccatgttgc
     1561 cgacttatga aagtttagga aagaaggcga ttattcgttt attcaatacg attatgatgc
     1621 gagcgggtgt tactttggtg attacgactg catttagcat ctccacgatg tttttcaata
     1681 tttcggcaac ctatccattt ttcatggttg cttttctgca aattgtgacc tttgccggta
     1741 tctatttcaa attgggcgac attatgagta tgttcaacct ccagagtaat gatagccagt
     1801 caatgggaag acgcgtcatg cgaaaaccgc aaatgttaat gaatcgaaaa ttgcggcaac
     1861 tcaatcgaaa tgtgggacga gctttggctt ttggtggtgg tgcagcagtc ggaaataagt
     1921 tggcaaagga acaatcaaaa tccaaattta agccatccgg ttcttctcta cgaaagaatt
     1981 cacgattacc gaacgatcac gaaatactat ctgacttaac taaggagaat ccagtttcaa
     2041 ataataagaa acaatcccga atgaatttgg ctgggagaaa aatcggcaaa gttttggata
     2101 cccaagcttt ggtgaaagat aagactaaac aagtaaagga tcaggtaagg aatacgccaa
     2161 cgaatttgaa atacaatctc cataaaggaa tcgaaaagtc gaaaaaagca ccagaagaat
     2221 tcaagcgcgg actgttccaa gaaaaagcgg atcgagtgga tttgcgagaa aaacaacgac
     2281 aacgaagaaa tgcgaaaatg gacgaaaaac gcaagatact gagtgaagca acaaatcgtc
     2341 agggaaaaag aagggcgaat attccaatag ataaagcagc tcaacctcag aaagatatga
     2401 tgctgaaaaa agaagcacca aagcggattg tgagagcaaa tctgaatccc gaaatcaaac
     2461 gaaaactaac tagtcaagaa atcatttcaa aagggaaaag ccaatcagca agtaagaaaa
     2521 attctttcca acaagtgaaa cagaggtcca ctctgccaaa gcagaccaat caaaaagtac
     2581 aaaaaagaat gaaccatcgt ccaaaatcaa ggtcaggtga gaaaaaatga acttgaagaa
     2641 agtgggtgtt atttcattaa cttttctgac catctctttt atgggtattt tgatgtgtgt
     2701 gggtttgttg tttggtgaag acagtgaggg aaatggtagt tctggcactt ctcctggcgg
     2761 aactagtgtc tcagaagaag tattgaaaca ccgtgcaatg gtggaaaagt atgccaaaga
     2821 atcggatata acagagtata ttccaattct tttggcgatt attcaagtgg aatctggtgg
     2881 cacaatggaa gacgttatgc aaagttctga gtcagccgga ttaccaccta attcattaag
     2941 taccgaggca tccatcaaac aaggctgtct ctattttgcg tctttggtta agcgttccaa
     3001 agaactaggg tgtgatcaag atagtattat tcaggcctat aactatggtg gcgggtatct
     3061 agatcatgtc gggaaaaacg ggaagaagca tagttttgct ttagctgaat ctttttcaaa
     3121 ggaaaaatca ggtggtcaaa aagtcgatta ccccaaccca atcgcgatta aggaaaatgg
     3181 tgggtggcgg tataattatg gcaatatgtt ttactgttta ttagtgaagc aatacctaac
     3241 tacaactcag tttgatgaca aaatagtcca agaaattttt gatgaggcat ttaagtatga
     3301 aggaacggcc tatgtgtttg gtggctcaag tccagaaacg gggtttgatt gtagtggctt
     3361 gacccaatgg tgttatgcca aagttgacct gaaacttcct cgggtggctc aggatcaata
     3421 tgatgccatg actcatattg atctaaaaga tgccaaacca ggtgatttag ttttctttca
     3481 ttcaacctat gatgccggta cttatgtgac ccatgtgggc atttacgcag gggagaatcg
     3541 catgtttcat gcaggagatc cagtggggtg gacgaatcta acggaaaact attggcggca
     3601 gcatcttatc ggagctggcc gatacaaata agagaaaaga ggaaaaagaa tgaagataaa
     3661 aattgaacgg aatcaaaagg aaaagagccc aaaaaagaaa aaagaacgag ttgtatccgt
     3721 ggg
//
LOCUS       DACEPW010000211         3713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEPW010000211 DACEPW010000000
VERSION     DACEPW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.216"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..249)
                     /locus_tag="IW819_002490"
     CDS             complement(<1..249)
                     /locus_tag="IW819_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245576.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFFIGLPYLCVLALAKL"
     gene            complement(262..480)
                     /locus_tag="IW819_002491"
     CDS             complement(262..480)
                     /locus_tag="IW819_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245577.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(477..1469)
                     /locus_tag="IW819_002492"
     CDS             complement(477..1469)
                     /locus_tag="IW819_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1245578.1"
                     /translation="MKKRQLRRKEKVYTEKVPNQRKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSQSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQEILKKEFYAQNFIQEPGSVSGIRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMDPENKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKTFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDRKGDERK"
     gene            complement(1548..2459)
                     /locus_tag="IW819_002493"
     CDS             complement(1548..2459)
                     /locus_tag="IW819_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1245579.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLSDVETELNGGTTEKPAEPEHLQPEKPEKEPVAPEPEIPESTLPTDPIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSETNTDSSSTEQPTMSFTSEEPKEEPKQPAEKSEAIPK
                     ENKPIVPAKEVTVSVTPNGEITTNNSQGMSVPIVTSNVEELTHIPTPTTPLKAEGGQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVLS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(2478..2822)
                     /locus_tag="IW819_002494"
     CDS             complement(2478..2822)
                     /locus_tag="IW819_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245580.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLGPRTERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(2842..3399)
                     /locus_tag="IW819_002495"
     CDS             complement(2842..3399)
                     /locus_tag="IW819_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245581.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIRVIKKLCTIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFHKREKNQMSAEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEKLMMDFSDLYRGYECSVIQER"
     gene            complement(3479..>3713)
                     /locus_tag="IW819_002496"
     CDS             complement(3479..>3713)
                     /locus_tag="IW819_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245582.1"
                     /translation="AYFYDDVRMRLVEQRMEIENDRYYFINYCKKRKYQVTDDFYDCI
                     LQDGEVILSKIAPSFRELVPINFLKKCHLRILP"
BASE COUNT         1075 a          767 c          544 g         1327 t
ORIGIN      
        1 tagttttgcc agtgctaaca cacataaata gggtagccct ataaaaaaaa caacccctgc
       61 tggtggaaaa aaagaaataa cccaaccaat aaatgaacgt aaaaataaat attcaaagat
      121 tacgaccaat aaaaaagttg ctgcattgac taatgcaact ggaaatggaa gacgccaatt
      181 gcttgtaatc tgttgtacgc gataaggctc cgcataaaca atcgaataat cgtaaggttc
      241 ccaatccact tcactatcct cctaatccat aaataatttt tcaatgattt taccaaacgc
      301 atcaaataca cgcgttggat catttaagaa gaaatagact gcggcacctg ctgcgataga
      361 tattaatgca tttcctactt ttgatttcat aaaatttcgc aatcccagat aaacaaccac
      421 cattattaaa aaatagccac cttgcgttaa tataaaatct acaattccct tcagtgtcat
      481 ttccgttcgt ctcctttcct atccaaccaa ttatgtgtga gtttttcaac ataatactga
      541 tttgtttttt tcgtaataac aagcgtcatt tgttcttttt gtaaaagtga cgtatcacca
      601 tctcgaaaga cgactgttgc ttttacaata aaagtttttt tcgtttcttt ataacgcaat
      661 actttcatct ctacttgatc aactttagct gtctgattga gtccttcagg ttctttcatc
      721 atgtatgaca tatcttttgc attgtttttc gcatacttat ctaagaaatc ttccacaaat
      781 ccttccacat ctttcgcttc cgacgcatca accgtttcgt ataatgtgtc atcataagta
      841 atcattcgtt gtttttctgt attctttggt agtgccgtaa aataaggata agcaataata
      901 cgacagccct tgtttgaaat agaaaaggga agattgagta cttgtgtgat tgtcacactt
      961 gtttctttct tattctctgg atccatcgag cgaatatcat aagttacttt ataactagct
     1021 gttggaatgg tttcaatttc ttttaatcca ataaacgaag cagcagttaa ttttcgaata
     1081 cctgaaacac ttcctggttc ttgaataaag ttttgtgcat aaaactcttt ttttaatatt
     1141 tcttgacgtt gttgttcttg ttcatgatta tcgaatgtat tcatatacaa agcaacaaaa
     1201 cgattcataa aggattcaat ttcaggtgtg tacgttaatt ctgactgact ttgtacttct
     1261 ttcttttgta attgttggat ttcatctttc aaaaacgaca cttgaacctt cgttttcatg
     1321 gattgaaaaa atgccaagcc ccctgaaaaa caaagaaata aaaccaaaca ccaaatccat
     1381 ttcactgctt tttttgaatg gattttcctt tgattcggta ctttctctgt ataaactttt
     1441 tctttccgcc gtagttgtcg ttttttcata agctactcct tctagtatgt ttttactagt
     1501 acgtacgatt gacaacgata agcttgtttt tctgaaaaat agtccattca attaccctca
     1561 ttttctattc cttttctctt tcttttatat ccaacaaatc cagcaatact tgtcaacact
     1621 cctcctagaa cagaaagtac aacatgtatt tcttcaccag tctttggtaa gacttttgtt
     1681 tttccatctg ctgttttgac ttcaatattg ccacttggaa gtttctttac aggaatcgac
     1741 aaatccttta ccaaattccc ttcactatct tggattaatg gaattccatc ttttacacca
     1801 acaattgttt gtccaccttc tgcttttagt ggagttgttg gtgttggaat atgggtcaat
     1861 tcctctacat tactagtgac aatcggtaca ctcattcctt gagaattatt cgtcgtaatt
     1921 tcaccgtttg gggttaccga tactgtgact tcttttgctg gaacaatagg tttattttct
     1981 tttggaattg cttctgattt ttcagcaggt tgtttcggtt cttcttttgg ttcttcactg
     2041 gtaaacgaca tcgttggctg ttccgtactt gacgaatcgg tattcgtttc agaagaactg
     2101 gttgttcctg attgttctgg agttgtgact atacttgaat ccgttgttgt tgaactgtgc
     2161 acaccttctt cttcaattgg atcagtcggc aaagttgatt caggaatctc tggttctggt
     2221 gccactggct ctttttcagg tttctctggc tgcaaatgtt caggttctgc tggtttttcc
     2281 gttgttcctc catttagctc tgtctcaaca tctgataatc cttcccctgg caatgtttct
     2341 acaatttctg acttaggtgt ttctacagtt tcatctgcat aaaccaatga actccctaat
     2401 tgaaacgaaa ataatgaaat tcctagtgtt gttacgactg ctaatgttgt ttttttcatg
     2461 attattcctc caatttttta tgcaattcgt gttttgttca tagacgccgc actaatatta
     2521 tctgaacgat aaaagaactc ccgtccgttt tcttctaacc caacatatgg attatttttg
     2581 aataattgaa tcaatagttc actggtgttg tattttggat agtttgcaaa aaaataatac
     2641 tgtgcttgtc gcccatccga aaattcaaca agtaattcat aacgataccg cttcaatcct
     2701 tttcgttgtt ttaaaggatc tctataccaa cttgtccatc gttccgttcg tggaccgaga
     2761 tagacacgat acacatagaa caatgccata ccacatacca ccaaaaataa taatcgccac
     2821 ataaaaattc ccccttttct tctacctttc ttgaatcacc gaacattcat accctcggta
     2881 gagatccgaa aaatccatca ttaatttttc gtataaacgt tgttgcaaat agcgattgtt
     2941 tcgaatacat aattcttcat agccatcaat cacttgatct tctttctcat agcatcttgt
     3001 agctctatga cgctcagctt gcatgacata accaaactct gcaccatatc gttctaccgt
     3061 taataactgt tgactacgca ataatgcaat ataacaatca caaaaatgtt cagccgacat
     3121 ttgatttttc tcacgtttgt gaaataggta ttctttagaa agcacattat tcgttaaaca
     3181 atctgataaa tagcgataat ctggcggtcg gttattttca tctgtgaatt tttcccataa
     3241 taaactgatt tttattcgtt cagcggctag atagtcttgt tcgcgtggat aaaattcatc
     3301 aatcgtacaa agctttttta tcacccgaat aaacaaatcc atataatgcg aaacgaactc
     3361 ttccttttca attggctctc ctaaaaattc cagtgacata ccatcttctc cttccttaaa
     3421 aaataaaaaa gcagaaaccg atttggcttc tacttttctt tccttttata gctctttttt
     3481 acggaagaat ccgcaaatga cattttttca aaaaattaat cggcacaagt tcacgaaaac
     3541 ttggagcaat tttagataga atcacttccc catcttgtaa aatacaatca taaaaatcat
     3601 cagttacttg atacttacgc ttcttacaat aattgataaa atagtagcga tcattttcta
     3661 tttccattcg ttgttctact aagcgcatac gaacatcatc atagaaataa gcc
//
LOCUS       DACEPW010000212         3664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEPW010000212 DACEPW010000000
VERSION     DACEPW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.217"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            181..1410
                     /gene="arcA"
                     /locus_tag="IW819_002497"
     CDS             181..1410
                     /gene="arcA"
                     /locus_tag="IW819_002497"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1245583.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            1501..2520
                     /gene="argF"
                     /locus_tag="IW819_002498"
     CDS             1501..2520
                     /gene="argF"
                     /locus_tag="IW819_002498"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1245584.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            2635..3582
                     /gene="arcC"
                     /locus_tag="IW819_002499"
     CDS             2635..3582
                     /gene="arcC"
                     /locus_tag="IW819_002499"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1245585.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
BASE COUNT         1272 a          643 c          775 g          974 t
ORIGIN      
        1 gaaagtgtat acagaataaa aaatgaataa attacgctta aaaatagaaa aacgtttaat
       61 tatttaatta tcgaatatta atgtgaggaa tgtctcattt tgaatgagac aaaattatga
      121 aagtgattac aaaaggagga tatcctatac atgtaatcaa aaagaaggag gcaacacaac
      181 atggataaac ctattcacgt tttctctgaa atagggaaat taaagacagt tttactgaaa
      241 cggcctggac aagaagtgga aaatttgaca ccagatatta tggatcgtct gttgttcgat
      301 gatattccat atcttccgat tgctcaagaa gaacatgaca actttgcgaa gacattgcaa
      361 aatgaaggcg tcgagactct ttacttggaa aaattagcgg cagaagcgat tgacgcaggc
      421 aatgtcaaag aacaattctt gaataaaatg ttggatgaat ctcatattgc atcgaatgct
      481 gtaagagagg gtcttcatga gttccttcta tcaatggaaa cgcaagaaat ggtagataaa
      541 atcatggctg gggttcgtac aaaagatatc gaagtacgtt ctagcagtct atatgatctg
      601 tcagcagacg atgactatcc attttacatg gacccaatgc caaacttgta ctttacacgc
      661 gacccttccg catcaatggg aaatgggatg acagtcaata agatgacttt tgaagcacgt
      721 cgtcgtgaat cgatgtttac agaatatatt ttgaaacatc atccaagatt tgcaaataaa
      781 ggaatcgaag tatggttgga tcgtgaaaat ccagatcata tcgaaggcgg agacgaattg
      841 atcttaagcg ataaagtagt agctgttggg atttctcaac gaactaatgc gaaagcttta
      901 gaaaaattag cacgccacct attcgcaaaa aattcaggat ttgaaaaagt gttggcaatc
      961 aaaattccga acaaccgtgc aatgatgcat ttggataccg tatttacaat ggtagatcac
     1021 gataaattca ctatccatcc agcaattcaa agtaaagacg gtaagatgga tgtctttaca
     1081 atcgaaccag atggtgatga tatcaagatt actcattcag ataacttaca cgaaacgatg
     1141 aaagctgctc ttggattaga tgatcttgta ctgatcccaa ctggtaatgg agatgagatc
     1201 gttgcacctc gagaacaatg gaatgatgga tcaaacacat tggcaatcgc tccaggtgtt
     1261 gtagtcactt ataaccgaaa ttatgtatcg aacgaattgc ttcgtagcta tggcatcaaa
     1321 gtgttagaaa tcaattcaag tgaattgtca cgaggtcgtg gtggtccacg ctgcatgagc
     1381 caaccacttg tccgtgaaga cttgaagtaa tttgtttcct gacggcagtc ttactgtcag
     1441 ctgccgtcgg gatttcctct ttaatcttaa ataaaaacaa tctaaaggag aatgtttaat
     1501 atgaaagaat cagtattcca aggaagaagc ttattagcag aaaaagactt tacaaaagaa
     1561 gaattacaat atttgatcga tttttcagaa cacttgaaag acttgaaaaa acgtggtatc
     1621 ccacatcact atctagaagg aaaaaatatt gcgcttttat ttgaaaaaac atctacacgt
     1681 actcgttctg catttactac tgcagcgatc gatctaggtg cacatcctga atatctaggt
     1741 gcaaatgata tccaattagg taaaaaagaa tccacagaag atacagctaa agtactagga
     1801 cgtatgtttg acggaatcga attccgtgga ttcagtcaaa aaatggtaga ggaactagca
     1861 gaattctctg gtgttccagt ttggaatggc ttgactgatg aatggcatcc aacacaaatg
     1921 atcgctgact ttttaactat ccaagaaaac tttggaacag tagaaggtat cactgttgct
     1981 tactgcggag atggtcgaaa caatatggct aactctttat tagtcacagg tgcaatttta
     2041 ggagcgaata tgcgtatcgt tgcaccaaaa gaattacaac ctgaagaaga aatcgtcaaa
     2101 atggcagaag gctttgctga aaaatccggt gcacaattga tgattaccga cgatgttgat
     2161 aaaggtgtag acggagcaga cgtcctttat tctgatgtat gggtatcaat gggtgaagaa
     2221 gataaattcg aagaacgtat caaattgttg aaaccttacc aaatcaatat ggaaatggta
     2281 gaaaaaacac acaacacaga tcgtttgatc ttcttgcact gcttgccagc tttccatgat
     2341 acaaacactg tttacggtga acaaatgaaa gaacgctttg gcatcactga aatggaagta
     2401 acagatgaag tcttccgcag caaatatgct cgtcaatttg accaagcaga aaatcgtatg
     2461 cactcaatca aagcgatcat ggcagcaaca ttaggtaact tgtttatccc tcgcgtttaa
     2521 gcataaccac attcatctca cgaaaaaacg agtcagcatt gagggaaaat cacaaaacgc
     2581 tggctcgttt tgttatctag aaaattcggt agaactgaag aaaggacgaa tgaaatggcc
     2641 aaacgaaaag tagttgtcgc tttagggggc aatgccatct tgtctacgga tgcaagtgca
     2701 aaagcccaac aagaagcgtt aatggaaaca gcaaagtatt tagtgaaatt tatcgaacaa
     2761 ggagatgaat tgatcatctc ccacggaaat ggtccgcaag tcggtaacct attgattcag
     2821 cagcaagctg ctgattctga gaaaacaccg gcaatgcctt tagatacatg tgttgcaatg
     2881 acagaaggat ctatcggtta ctggttacaa aacgccatgg gcgaagtact gaaggaaaaa
     2941 gggatcgata aagatgtggt ttctttagtc actcaagtta ttgtggatga aaatgatcct
     3001 tcattcaaaa atccaagtaa accagtcggt ccattctaca cggaagaaga agccaaagaa
     3061 caaatgaatg ctgactccac tgtgacattc aaagaagatg ctggacgtgg atggagaaaa
     3121 gtagtcgctt cccctaaacc catctcgatt aaagaagcac gtgtcatcga aacgctggtc
     3181 gatcaaggag ttatcacagt ctctgttggt ggcggtggga ttcctgttgt agaaacagct
     3241 actggattag aaggacgaga agccgtaatc gataaagatt ttgcttcaga aaaattagcc
     3301 gaaatcatcg atgcggattt gctgatcgta ttgacaggag tagacaatgt gtatgtcaat
     3361 taccaaaaac cagatcaaaa gaaattagaa actgttactg tttcagaaat gaaacaatac
     3421 attgatgaaa agcaattcgc tccaggaagc atgcttccga aagtagaagc agctattgca
     3481 tttgttgaag caaaaccaaa cgctaaggca atcatcactt ctttagaaaa tatcgagaat
     3541 cttttagctt ctgaagaagg aacgattatc gtagctgatt aatttgttaa gtctttcgta
     3601 tttacgaaat gatttatagc tatccaggtg gtatttcttc tcctttaggc catctggatt
     3661 tttt
//
LOCUS       DACEPW010000213         3621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEPW010000213 DACEPW010000000
VERSION     DACEPW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.218"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1295)
                     /locus_tag="IW819_002500"
     CDS             complement(<1..1295)
                     /locus_tag="IW819_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1245586.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLIVGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQFKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANIQTGDVLEKLYNASTLIPSICFFIVG"
     gene            complement(1415..2062)
                     /gene="eda"
                     /locus_tag="IW819_002501"
     CDS             complement(1415..2062)
                     /gene="eda"
                     /locus_tag="IW819_002501"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1245587.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(2155..3578)
                     /gene="uxaC"
                     /locus_tag="IW819_002502"
                     /pseudo
     CDS             complement(2155..3578)
                     /gene="uxaC"
                     /locus_tag="IW819_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
BASE COUNT         1194 a          742 c          477 g         1208 t
ORIGIN      
        1 cctacaataa aaaaacaaat tgatggaatt aatgttgaag cgttatataa cttttctaaa
       61 acatctcccg tttgtatatt tgctaaagag tcatagccta taatagaaag agtccaacct
      121 ccgacgcctc cagctagagc ttgacctatt tttctagcaa aagaatatac cgcatatacg
      181 gtcccatctt ctctttgatc agactttact tcttgatcat caataacatc agtaatattt
      241 gcccaaataa cagtattaaa cacacccaaa cccatatagc caaccgaact aagaattaag
      301 aatacataca tattactagt ttttatcaaa aataagatca aatatactat tgctgaaaag
      361 aacattccta ctgatgccag ttcttttttt ccatattttt tacttaaaaa caaagttaaa
      421 ggtgaaacaa ctattaaaac caaaatagga ttgataaaat tcattaaaac tattccctgt
      481 gcatcattat agtaatcagg aaatacataa ttattcatac tcataattaa taattgagca
      541 aggagtaaga aaatagccgc accaattata gaaagtaatg ccctattttt aactaaccct
      601 tttaaagatt ttaacaatga aactttgaat tgagattggt caattcttac tcgttcagta
      661 gttagtttat aacatattaa ataacaaata agtgcacata tcgaaaatat tcccgcaaca
      721 attgtaaaag ttgatccacc acgaactatt tgagcgcctg aatcatctct tgtataaata
      781 aataatggtg ttaatacacc aacgatcaaa cttgctaaag tagctccaac atttcggaat
      841 gtcgatagca ttgttctttc ttctgtaata gaagtaatag ctgatgccat tgatccataa
      901 ggaatattta tagatgtgta aaaaatactc ccccataata tatacgtaac atacatgtat
      961 actaccttaa cgctcataga agctgaaatc atagaagttt gatacattaa aaaacttgct
     1021 aaagcgactg gaccactcat tcttaaaatc cacggtttaa acttaccctg cttagttgct
     1081 ttcgatctat caacaatacg ccccatagtt acatcagtaa ctgcatcgat aaatcttgct
     1141 actaaaaata atgttccaac ggcagctccg cttatcccca atacttttgt ataaaagatc
     1201 attaaaaaag aactagcaaa aatgaaagta aaatcgtttc caaaatctcc aaacatatat
     1261 cctattttgt ctcttatacc aaattttctt tccatacaat tctctccctt cttttgttaa
     1321 gcgttttcat tcttatgaaa gtacgtacta tactctgaag cgtaaagagt cagtatttat
     1381 tttttacatt tcagagtatt atttgttttt tctcctaata attctttatc tcataaaact
     1441 tatctatata tttactagct actcgactta cttcttcaaa atcacctttt tcaacagaag
     1501 ccaataaatt cccacctaca ccaactgcaa caactcctac ttcaaaccaa tcttctaagt
     1561 tctctagact tactccgcca gtaggcatta tatttacgtg tggtaatgga gcttgatagg
     1621 cttttacaat ggatggacca aaagcagaac ctgggaatag tttaatgata tctacgccag
     1681 cagtaagtgc ctcctttatc tcagtaattg tcatacaacc tggtaaataa ggaatttgat
     1741 ataaattaca aatctcagct gtttgtcgat caaaagatgg actaacgata tattctgccc
     1801 cagccataat tgcgatacga gcagtaaacg catctaaaac cgtaccggct ccgattatta
     1861 catctttttg atcagcatat tttttagcaa tttctttgat tatttcttca gcattaggaa
     1921 ctgtaaaagt tagttcaatc cctttaattc cacccgcaat aattgaattt attgctttaa
     1981 tggcctcatc cttatttttc ccacgtacaa ccgcaatcac tccagccgct tctaacttac
     2041 ttaaaatttc tacttttttc atatctatct cctctatctc cttcttttat ggatacatac
     2101 tttgtctagc tgtactaact taaaaagaat aaatttaagc ttttatttca agtattattt
     2161 tctacttccg atttcaaaaa aattaaaata attatacgca ttacgataac aaatattctc
     2221 cactatctga ctaagataca tttcatcatc tggaattcgg cctgctttaa cccactcgct
     2281 tagtaattga cataatactc gacgaaaata ctcatggcga ggatagctta gaaaacttcg
     2341 agaatcagtc aacatacctg taaaattcga caacaaactt tgttgcgcca tcgtagtcaa
     2401 ctgttgacgc attccttcag ctgtatcatt gaaccaccaa gcacatccca attggatttt
     2461 ttgaatcata cccccttgga agctttgcat tgctgttgct agtggaagcc aatcagttgg
     2521 atttaatgaa tataaaattg tttttggtaa gcacccactc ttttcagcgg tatcgaacaa
     2581 tgctacaaca ttttgtgtca aactagcttg atcccccatc gaatcaccac cgacatctac
     2641 tccaatttgt tcctttaata tacttgaatt atttcttaaa gcatttaaat gcatttgcat
     2701 cgtccaacca tatcttgtat actcagacat caaatgtaat tgaatacctg taacaaattg
     2761 tgcaatttct gataaatgga aatccgtttt tcctgctaaa gcttttatta acagattgtc
     2821 gagtattttt tcatctgcta tttcataaaa ataagtattc aaaccgtgat ctgctaatcg
     2881 acagccaatt tgatgaaaat gatccactcg ttggctcaat gcttgcttca acccattaaa
     2941 gtctttgatt ggtatagccg ctgcttgact aagttttaaa atatagttcg aaaagcctga
     3001 tgatttgata ctccagacct tatctggacg gaaagtaggt aataccttga aagaattatc
     3061 ctgtttagaa agctcagtat gataccccaa gtcatctgta ggatcgttgt acagatggct
     3121 ttcacatcca tcatctccaa caattctttc gggcgatagg aatccgtttg gattttttcg
     3181 ttagcacgtc gccaaatttc tggtgcattc ttctcagaaa ttgtcaaatc aatatcaaag
     3241 taacgttgta attccaaatg agaccattca aaaataggat tacctggcgc cttctccact
     3301 gtttttacaa aagctaaaaa tttttcataa tcatcacctg gaccactgat caactcttct
     3361 tctataccat tagctcgcat caatcgccat ttataatgat ctccagctcc tttatcattt
     3421 aaccatacct gagtcaaatt cttgtattgc ttattttcta gcaaatatgc tgggtctaaa
     3481 tgacaatgat agtcgataat tggttggtgt tttgcaaatt tatgaaaaag tcttttagct
     3541 atttcattct ctaataaaaa gttcttatct aaaaacatat ttaaacactc ctttccttcc
     3601 gttagttttt agaagagaaa a
//
LOCUS       DACEPW010000214         3589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEPW010000214 DACEPW010000000
VERSION     DACEPW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.219"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(60..269)
                     /locus_tag="IW819_002503"
                     /pseudo
     CDS             complement(60..269)
                     /locus_tag="IW819_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(418..3405)
                     /locus_tag="IW819_002504"
     CDS             complement(418..3405)
                     /locus_tag="IW819_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ1245588.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
BASE COUNT         1119 a          634 c          617 g         1219 t
ORIGIN      
        1 tttaaagact attaaatcaa tgttaggaaa gactattagt aaaactatga attattcagg
       61 aaaaaaggtt cttttttaaa gttttctatt gcttcctggc gttgataaat gagatataaa
      121 gtcggtgttt gtacacgtcc taatgaaaat gtaccttgta cgcctttttg ttgtaaattc
      181 aaggtatata aagggcttgc gttcatgccg atcaaccaat cggcaatttg gcgtgtttgc
      241 gcttcttgat aaaggggtac atggaacata gaaacgaaat agtgcggtag cacaaaagta
      301 aaaacacaag aaacgttgat ttaacgctgt tctcgtgttt ttctcttttt ctattcgaag
      361 tgattcgctg gaacagtcat acgaaatagt gcggtagcat gcaaaacccg aacattgcta
      421 gaattcttct acattcaatg gacgtaactt accgttggat aattcagctt cgtatctaaa
      481 cgtatactta ccaacaaaat tgatgtgatc actaattagt ggagattgcc tgcgtatcat
      541 ttcttcggaa cagtcgaatc cttcagcttt catttgttca aatacttttt ctaaatacag
      601 tgagttccaa tatatgatgg cattagtaac taatcctaat gcattgagct gctcttccat
      661 cccatcataa taagtctggt agagttttcc ttttcgtcca taaaaaatat tacggcataa
      721 ggcatgccga gcttccccct tattcaattg ttctaaaatt tgccgtgcat atatttcgtc
      781 agaaatataa cgaagctgat gtttcgtctt ataaactttt ccatactcag ttattgcttt
      841 tcctaaaggc gtgggttgcc cattccgttg aagagatttc gttaattctg ttgcattcac
      901 ttttccagat ttcaacgaac ctgcaaccct aagaatgtcc tcccaatggg tataaatcag
      961 ttccatatta attgaatttt gagacaacgt atcaagttcc ccgtagtgag cgttagcgtc
     1021 gattcgccaa agtttggttc ctttatcatt ggcaattctt ggactaaatt gaaaccctaa
     1081 taagccaaag agaccaaaaa tcatatcgct ataaccagct gtatcagtca tgatctgatt
     1141 aggagttaat tcgctgggtt gattgaggaa tccttctaga aggtaaagcg aatctcgcag
     1201 tgtccctgaa acgaccattc cgtggaaccc tatatattga tcagagacaa aattgtagaa
     1261 tgtgatccca cgaccacggc caaaatattt tggattactt ctagaatata gggaacgttg
     1321 tggtgtgata taacgaatac cgtcagctga agccatatgc ccatcacccc atatcttaga
     1381 ggtggcaagt ttcctgtgtg catgtattat tttttgatta gctcctgaaa gtgtatccac
     1441 tcgaatataa tgatgattga catagacaag acgatcatac tttaaagaat caaatccttc
     1501 ttttgaaacc ggggaaaaac caatattgca ggactctgat aaaagaactg ctaataaact
     1561 aatatccaag tctttcattt tggtgtcctt gtcacttaag tggttaaatg attgagtaag
     1621 atttaactgt tggtttactt ccaataacaa atctgacaaa tcaattttag gaagtaactg
     1681 ctgaatgcga cgtctaaaag acttatcttc ctttttttca tatgtctttt taagtctaga
     1741 gacaaccagg cgatctgcat tttctattct ggccatttta gaagtaggcc aacgatttag
     1801 cgcttcttta taagacagtt ccaaatcgtt ctttaaccat aaagacattt catttgctga
     1861 agaaggaaga tctagttgtt ctaataaaac gtatttttgt tgaacccaag ttttctcatc
     1921 aatcaagcaa tccataggat tgttatataa gacactctga ggaatataaa tatcatggcg
     1981 ttttaaagat tgaattaaca attcgacagc tgcaatcaat acacatttat tagtttgttc
     2041 agggtattca tgaatgtaat aaatccattt ctttggaaga gtatgtttaa tagctccaaa
     2101 cattttgtaa gtaatcggct taggaaatct attattaatg aaggtccaga gttccttaca
     2161 gttgttaccg tagtcattgc tgtcaaatgt tagtgtagtt aaaatatccg gaataaattt
     2221 tctgaacttt cgataagtgt tcagaagttc agaaatggct attggttcac gatcattttt
     2281 aaccaatagc cgaacttgcg atacagcttc ggtaacattt tcctcagaat atttttctaa
     2341 gatacgatcg cgaagggata gttcattagt atcagaatga ctggacatta ctaaatctac
     2401 aatatctgaa agtgtcgatg cggctcgatc tagatctttt atggtacgaa gacgttcttt
     2461 agattcctta tttttggcgc gtctaaaaac agtttcaaaa aaattagata gtgctaaaat
     2521 ttgttcatcc atagccttac gtttgtatat gaaaataaat gccactaaca acgccagttt
     2581 tttctgttca ctcattcgtt gaattaattg agctttagct ttaaatgcat aatttgcaag
     2641 aacttttatt ttcccttcag gaatagattc catcttccat ttttcagtag agaaagcttg
     2701 aaattgcttt agtcggtcaa aacctctggc tatttcttta tgactatcgt cagctaacgg
     2761 ggttctcaaa atgtccattt tgattattgc cccataaact ggagttccaa ttaattctaa
     2821 atgattgaga agccgctttc tctcatcttc agttggaacc aaagacaatt gtcggtataa
     2881 atgctcttca gctttttcaa ttgcactagc aacaaaacga gaaaaagtag taattccagg
     2941 taaaacaatc ttttcatcca gacatttttt tagaagcata tcaaacaaca tatctgaaga
     3001 ttctgttgta tacagcgctc tatttaaaag ccagttagct aggtatgttt ctatttccgg
     3061 ttggttgaaa tcttgatatc cataatgtga ttttatcaat tttgtgtgct gatacctggt
     3121 ctgtctcctg ctatagtaga taatttctcc aggatcaata tttagttgag ttgctacata
     3181 actcaataca ttttggggaa tattggtaag atctaatata aaagttccta aaaaacgtag
     3241 tgagactaac tgtagtgcaa aacctaatct agtagctggt cgtcgtatag tctccatcaa
     3301 ttctaaatct ttttcatgca aataaaaaaa ttggtttaat tgttctgtac taggttctcc
     3361 taaataaatg ccaaatgctt ttttgttact tttgatttca tccatttaaa ccctccatga
     3421 agtaaatttt aagtctacat aaaatttatt tttaaaaatg ctatgatagc agtaaaacta
     3481 caagtaaatt ttacatgtaa attatatcac ttttaaaaag gagtttttta tgggaaaaat
     3541 tggctatatt cgagtatctt cacaagatca aaatttggat cgacaattg
//
LOCUS       DACEPW010000215         3583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEPW010000215 DACEPW010000000
VERSION     DACEPW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.220"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(130..765)
                     /locus_tag="IW819_002505"
     CDS             complement(130..765)
                     /locus_tag="IW819_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1245589.1"
                     /translation="MFFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(1031..2083)
                     /locus_tag="IW819_002506"
     CDS             complement(1031..2083)
                     /locus_tag="IW819_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1245590.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     ILVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(2096..3034)
                     /locus_tag="IW819_002507"
     CDS             complement(2096..3034)
                     /locus_tag="IW819_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1245591.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1223 a          747 c          539 g         1074 t
ORIGIN      
        1 atgatcagtg atagaagcag tttatctata caaaaaaaga agggttctat gatatttggt
       61 gtccttactc gccttgagta acaacaccaa atatcataga accacttaat cagcttcaca
      121 ctgctttttc tactttcgta gttgaaaaaa acttaaacaa atcatcggca aatagttaaa
      181 gaaaaaactt aatcgttgaa agaagcccaa ttgcttaaag aaggcaaact gactaatcga
      241 cggaatccgt gctaatccgt aaagacctgc aattagtaaa ctaataactg ttaaaacaag
      301 ataaaaatgg ctaagcgtac cttgaccact ttgacgataa agcagaacaa gtaacaatgg
      361 gaaaagtaga aagcctgcat agcctagccc agaacctata ttatggatcc aagtcgatag
      421 attccaagag ctttcattcg tattgatact gaataagcca gtaaaaatac aatcacctat
      481 cccatataaa ccaactgttg caacaacaag tattgcaagt gtttttgatg ttccaacaaa
      541 taggtgatac aacgcaggca acgaaagaac aaaaaaaagt ccagatacaa ctgaccagac
      601 caagaaagca cgtctgactg gactatctac gtcaccaaac acactaatta cggtagtcat
      661 ttgattcagt ttgggataaa aaaggccaag gatatacggt gtcgataaat cactcatcac
      721 acctaataat aaaaaataaa aaccatattt ttgcaaaaaa aacattttct tcccctactt
      781 tttagtgatt attcgataca tccaacaatc aaattttttc tcattttaac acatattttt
      841 attctagttg tcttttgaac aatcaataac ataaaggtga gtttcttcta cattttaaaa
      901 aatcgaatgg cactgaagag ttcagttgtc agcatcaatc tcctactcaa aaaggcaaaa
      961 aaacgtaagt cagatgttgt tgacatctca cttacgcatc cattgattga aaatttctta
     1021 gtaaaatcta ctagcctaaa aatttgaaga cttcttctcg atcatacagt ttaaatactt
     1081 tctcgcataa aaattgagta attagtgccg caaaaacagg gatgactaac caacaaagaa
     1141 caacaagaaa gccatttagc cctgcatcta aagaagccaa cggaccaaca agtccgacca
     1201 cgccgaaacc agcagaagca ggcgtacctg aaatagaaaa tagagcgaca agaatagctg
     1261 taatcactgc actaactaaa caaggaataa gaatcacagg ttttctaaat agattaggca
     1321 tcatcatctt catagcgcct aaagcaatcg caattgtcac acctgactta ttaaccttcc
     1381 aagagttcac tactaaaaca aaagtggttg cagctacgcc catcgcagcc gcaccagcag
     1441 aaacaccatc tagttggatt gccattccga ttgctactgt tgaaatagga gagataatca
     1501 aaaaggcaaa ggaacaacca atcaagatag acattaacag cggttgtaag gtagtgaagt
     1561 tattaatcaa tgatccaatt gcagaagtaa ttgctgttac aaaagggaat aaataaaaac
     1621 caagtaaacc tgcacctaca cctacaacaa tcggtgttaa cacaatcgac actgacccaa
     1681 aactatttcc aatcagtaaa atcagcccga cagcaatcga tgctgtcagc atcgtattaa
     1741 taatatcgcc tgtaccagca ccaacgtaaa cacccgtttc acccaaatca ggtgtgaatt
     1801 tgactacacc agaaccaaca aaagaagctc cggctacaac catcattctc atcggatcaa
     1861 atccaaattg taaggcaatc aaaccaccaa tgatcaaagg tgttgctagt tgaaaaataa
     1921 ctgctaactg tgcgatcgtt accgcaaagg gatacgcact aaaatatttt aaaatggcac
     1981 ctaatactgc attaggaatc aatccaataa tgattcctgt tgctgtacca gcgaggactt
     2041 tattcagaaa aattttcggt gatatttttt gtgaagtttc cattcaatct ctcctttatt
     2101 ttacgttcaa actatcttct tttgcatgga caagctcagt caagaaatca caataaggcg
     2161 tcgcaacacc atatttcttc ccttttctag aaattgcgcc attgatataa tcgatttctg
     2221 ttaatcgatg gttcttaatc aagtcttgat acattgaagg ataatgtaac ccaattgttt
     2281 ccgggtcaaa acaagattca caatgtgcaa tgacttctgg gacatctagt tcaatcttct
     2341 ctactgctgc tactttggca aactcattga caatcgtcgc caccatttta tgagcagtcg
     2401 atgtttttcc tagctcagcc atattaacgt ctaagattgt gcatagtccg ttcattgttc
     2461 cgttcacaca tgctttgcga taaatagaat agtgaatatt gtcagaaaaa tgagcattta
     2521 atcctgattc agacaatttg tctgctaatt tttttgcagc tgcttctttt ccatcaccta
     2581 aattttgtaa ttctactgaa ccacttccaa ataatttgac ctgaccagga ccttcaagac
     2641 ctgccgtcca catggtattt ccaatataga tattttccat tggtacaaat ttctcaataa
     2701 tatcttcatg cccgatacca tttaatagac ataagacctc tgtatctttt ttgattaatg
     2761 attggatatc ctgcagcatt ttttccagct gcatcgcttt ggtaaataga ataatcagat
     2821 caacttgatc ttctttttct acttcggatt gaagaacgat tggtagtttt gcttctacct
     2881 ctttaccatt aaaatttgct tgtaatccat gttctttgat ttgttggaca tgttctgccc
     2941 agccatcgat taataatact tcgttcccac tttggtgaag catcaaccca aagcgacttc
     3001 ccattgcgcc agcgcctgca attgctattt tcattgattc ttcctccttt ttctttctat
     3061 tatactcatt tgagaatctt ttcacaacct cgttttgtta ttcttctctt atttatcatg
     3121 tttatatcca ccattcaagt tattttatca gcaaatcaat aaaaactgga atagaatccc
     3181 aatttcatcg taagagaaac tagactagca ataagaaaat agaaacattc atagaaaaaa
     3241 tctcactcct ctgattataa aagagggggc attaacctcg tgtatactaa aaccttccat
     3301 tcattcgatg gatgattttc tttttgtgaa aaaatattca ttgtagtacg atgttgcgca
     3361 acacagacgg atggtattgc ttattattta ttaaaatcga acaatacact tatttaaaaa
     3421 cttctttccc agattttctt ccatttttac agttataact agtattatca tgaaaaatag
     3481 ccttttattg tctatgggaa taataattta aaattggtac ttttttatta attagttcat
     3541 cgcccaataa tctctccagt ttcttagcat ctcctgactc aac
//
LOCUS       DACEPW010000216         3561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEPW010000216 DACEPW010000000
VERSION     DACEPW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.221"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(254..541)
                     /locus_tag="IW819_002508"
     CDS             complement(254..541)
                     /locus_tag="IW819_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1245592.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="IW819_002509"
     CDS             complement(561..785)
                     /locus_tag="IW819_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ1245593.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1425..1958)
                     /locus_tag="IW819_002510"
     CDS             complement(1425..1958)
                     /locus_tag="IW819_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1245594.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="IW819_002511"
     CDS             complement(1979..2923)
                     /locus_tag="IW819_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ1245595.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT         1011 a          639 c          544 g         1367 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa t
//
LOCUS       DACEPW010000217         3554 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEPW010000217 DACEPW010000000
VERSION     DACEPW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.222"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(300..797)
                     /locus_tag="IW819_002512"
     CDS             complement(300..797)
                     /locus_tag="IW819_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1245596.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(837..1646)
                     /locus_tag="IW819_002513"
     CDS             complement(837..1646)
                     /locus_tag="IW819_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1245597.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1980..2837
                     /locus_tag="IW819_002514"
     CDS             1980..2837
                     /locus_tag="IW819_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1245598.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2830..>3554
                     /locus_tag="IW819_002515"
     CDS             2830..>3554
                     /locus_tag="IW819_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1245599.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRN"
BASE COUNT         1168 a          621 c          641 g         1124 t
ORIGIN      
        1 ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg aaatcagcgt
       61 tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
      121 aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg tgagcctgcc
      181 gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac gtaggactaa
      241 aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga tagatatttt
      301 tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga ttctccgtgt
      361 tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa agcactctgc
      421 aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac ttcgatctct
      481 ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt ttcaggtgga
      541 atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag aagagggatc
      601 aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat atcccgtgtg
      661 aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat acgttctgcc
      721 aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg attttgcata
      781 tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta ttctttttaa
      841 tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt tacttggttt
      901 gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc gctgtaattc
      961 aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga ttcgattcca
     1021 aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg cgtgatttct
     1081 aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc tctttgtagc
     1141 ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct aagagaagga
     1201 tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa atgttcataa
     1261 taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc ttgggtttct
     1321 cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt tctcagtgta
     1381 tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt catttcagca
     1441 atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga aaaaatatct
     1501 ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt ttcatcaaat
     1561 ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag caaggtaccg
     1621 tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac atatctattt
     1681 tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg ctttgtcatt
     1741 atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta gtttttggtc
     1801 aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga tgcgttttgt
     1861 gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt taaaaccaaa
     1921 tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga gtgattttga
     1981 tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat tatgctcctt
     2041 acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga cgcataaaat
     2101 tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa gaaacggtaa
     2161 ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat atctacgaag
     2221 acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct ttaccaaact
     2281 tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg ttagtactag
     2341 acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc gctattatcg
     2401 atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac ttgtacttca
     2461 attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt acagaaaaaa
     2521 ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa gatgctttga
     2581 atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg cagacttcgc
     2641 tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat aaagaaggaa
     2701 atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat acgttgaaag
     2761 atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta acgaaagtag
     2821 gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac aaaattcgta
     2881 ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc taaatttgtt
     2941 cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt acgcttctca
     3001 gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa agaattcttt
     3061 acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat tgcgattccg
     3121 gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga catgatcgct
     3181 ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt agcagctgtt
     3241 gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa atcaatgcgt
     3301 tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga aaatattacg
     3361 caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca tgaccaagat
     3421 gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg gaatgcacaa
     3481 ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg tgaatacgaa
     3541 gaaactcgca acaa
//
LOCUS       DACEPW010000218         3546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEPW010000218 DACEPW010000000
VERSION     DACEPW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.223"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..1615)
                     /locus_tag="IW819_002516"
     CDS             complement(83..1615)
                     /locus_tag="IW819_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1245600.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            1849..2550
                     /gene="nagB"
                     /locus_tag="IW819_002517"
     CDS             1849..2550
                     /gene="nagB"
                     /locus_tag="IW819_002517"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1245601.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            2804..3517
                     /locus_tag="IW819_002518"
     CDS             2804..3517
                     /locus_tag="IW819_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1245602.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
BASE COUNT         1065 a          623 c          731 g         1127 t
ORIGIN      
        1 attggccacc atttattcag aagaaggagt tgtgacaaaa cttttgtcac agctcctttt
       61 tttcaactat tcaatcaaac gtttattttg gttcaatctc aatgactcgt tgcacaagat
      121 cttgataaaa ttcttgttca tgagagacga tgattactgt tcctggaaat ttctgtatcc
      181 cgtcttgtaa gctttctttc gtttcttgat cgatatggtt cgtcggttca tcgagaatca
      241 gtaaattacc ttggttcatt gtcaactgtg caagtttcac cttagtttgt tccccaccac
      301 ttaatgtagt gagaggttca cttgctaatt gattaactaa ccctgcttta gctagttggc
      361 gacgtaattc tttgatagtc gcttttggaa attgactgct taaatattgt agtggtgttt
      421 ctaatggatt ttcccagtac agatcttgtg aaaagtaatt gatttttgtg ttatctggga
      481 agtggaaatc gccacttatt ttttcaatct caccgatcag tgtttttaat aaagtagatt
      541 ttccaatccc gttgaagcct cgaatggcaa tattttcacc aaagcggatg gtcaaattga
      601 tgggtgcaag tagcggtcga tcatatccga tgactaaatc agttgtttcc actgctaatg
      661 tcgtgacaat aggcgagaat ggaaaatcaa agacagcttt acttgctgaa gctggaggcg
      721 tcagccgttc gattttatct aattgttttt gtcggctttt tgccatcgtt gatcgggaac
      781 cggctttgta ttttcgaata taagcttcgg tcttttcgat ttgtttttgt tgagcatgat
      841 attggcgcaa atagctttcg cgattttgtt ccttttgttt caatgcttgt tttagatggc
      901 cagtatactt agtaatcttt ccaaactcga tgtctgcgat atgggtggtg atctgatcaa
      961 gaaattcttg gtcatgggaa atcacgatat acgcaccgtc aaaatcctga agaaactgcg
     1021 tcagccattg gatatgctga tcatctaagt gattggttgg ttcatctaag atcagaacat
     1081 ctgggttttc taaaagtagt tttgctaaaa tcacctttga acgttgtcca ccacttagat
     1141 ttttcaacag tgaatctaaa cctaagacat cgatccccaa cccgctgctc atctcagcaa
     1201 ctaaggtgtc gatttgataa aacactcctt ggtctaattc cgtttggagt ttcccagctt
     1261 tttccagcaa ggaatcatcg aaggtctcgc tatatgtttg gtaaagaaaa gcaatttttt
     1321 cttccttttc gtattctttt gcgtacgcag tttttagaaa ttcttcgatt gttaatgttt
     1381 catcgacttc cacatattga tcgaggtaac ctttgtgaat atttttttgc caatggatct
     1441 gtccgtcatc tggtaagatc tcaccagtga tgattttaat caatgtactt ttaccggctc
     1501 cgttttgtcc aatcagccca agatgttcgc cagcatttaa ttgcagatcg acaccttcat
     1561 agaggatttt gtcgccaaat tgctgggtaa tgttgtttaa tgtcagtata ctcataataa
     1621 ctcctttttc ataaaaaaac tgcagacata aagcgtccac agtgaatcac ttgcttggtt
     1681 ttcatgcaac atggattgtt tgattgaacc atcttatgaa acctaacacg gattcctatt
     1741 tattgtcaag aaaatgaaaa aaatgcaatt tggtctatac taattattgt aaaaagaaaa
     1801 aaagaatgct ataattgggc tataccaaaa tataaaggag caatcactat ggaaatcatt
     1861 cgtgtaaaaa acgctgaaga aggcggaaaa aaagcatttg aaatcatcaa agaaggtatg
     1921 aacaaaggtg caaaagtatt aggattggca acaggaagca caccagttac tttatataaa
     1981 gaaatgactt caagcgattt ggatttcagt gaaatgactt ctgtaaactt agatgagtac
     2041 gttggactag gcggttcaga tgaacaaagc taccgtcatt tcatggatgt ccaattattt
     2101 gataaaaaac cattcaaaga aacgtttgta ccagatggaa aagcagaaga tctggaagca
     2161 gcatgcaaaa agtatgatca aatcatcgat gcacatccaa tcgatatcca aatcttagga
     2221 atcgggcaaa atggacatat cggtttcaat gaacctggtg cttcatttga cggaacgact
     2281 tcagtagttg acttaacaga atcaacaatc aatgcaaaca aacgattctt cgataaagca
     2341 gaagatgtac caacaaaagc tgtttcaatg ggaatcggtt caattatgaa aagtaaagaa
     2401 atcgtcttga tggcatttgg cgaaagcaaa gctgatgcaa tcaaagggat gattgaagga
     2461 cctgtaacga atcatttgcc agcaagtgta ttgcaaaatc acgacaatgt tgttgtgatc
     2521 atcgatgaag aagcagcaag taagttgtaa ttttcaatag taggctaaag aggttgtgaa
     2581 atcagcgttt tgacctgagt attagagaag acaaaggaaa aatagctccc catattttgc
     2641 cgtttgttga gtaataccga gggtcagctg attgaacacc gtttattcat aaaaaggact
     2701 atgacaaaaa ttttgtcaca gtcctttttt cgtaagaggt aaaaattgca ctttttttgt
     2761 ctgctccttt cagacatgat atattggact ggagggtgtt tagatgaatg atatggtatt
     2821 gacaattcca gagattattt tacgtttagg gatcgctatg ctatttggcg gagcaatcgg
     2881 ttttgaacga caatataaaa atcgtccagc aggtatgcgt acgcatattt tagtttgtat
     2941 gggagcatgt atcattgcat tgatccaagt tgagatcgct agtggtgcat taagagatgc
     3001 tttagagtat ccacagcttg ctggaacgat tcgttcagat gaagcaaggc tgattgctca
     3061 agtggtgagt ggtgtcggtt tcttaggcgc gggaacgatc atcgtcacga aacaatcggt
     3121 gacgggactt acgactgccg cttctttatg ggcaattgct ggattaggga tcgccatcgg
     3181 catgggttac tattccattg cgatcatcag ttttatcggg attgctattg cactcacagt
     3241 agtgaaacgc gtgatccatg tacctacgac taaaaagtta gaaatccaat ttatccatag
     3301 aaaagaaacc aaagattttc ttaccgatta ttttgaagag aaaaatatcg taatcgaaga
     3361 tgtgaatttt gacgtaaaat tcgttgatga ctaccgagtg tacaaaaata tctatacgat
     3421 tgatcttcca aaagggctca cttatgccga tgtgatcgag gagttgtccg tacacaaaaa
     3481 tatcacaaag ttgcatttag tcagtattgc gcaataaatg atctgaggtt gtaaaataga
     3541 tgtcct
//
LOCUS       DACEPW010000219         3525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEPW010000219 DACEPW010000000
VERSION     DACEPW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.225"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            180..776
                     /locus_tag="IW819_002519"
     CDS             180..776
                     /locus_tag="IW819_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1245603.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            900..2699
                     /locus_tag="IW819_002520"
     CDS             900..2699
                     /locus_tag="IW819_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1245604.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2977..3189)
                     /locus_tag="IW819_002521"
     CDS             complement(2977..3189)
                     /locus_tag="IW819_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245605.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          998 a          607 c          631 g         1289 t
ORIGIN      
        1 ttttaaaaaa aacaagttat taaataagtc tttagttgga tgtttttttg agatcaatct
       61 tttatactta tgatgaactt acaaaattgt ttgttttctg tcatatcaaa aaatggcgca
      121 gttagttgct tttctagtaa agtatagtta acaaagaacc tctaaggagt tgatgaataa
      181 tgaatattgc tgcctctcga aaactgataa atttcatatc cattatcgga ttaggcctat
      241 caatcgcgct gacgatttat tttattaatt taggtgtttt caaagatttg aatgcgttaa
      301 gaggattagt aggtgattcc atcattttag gaccgattat ttttgtcttc atccaaatcc
      361 tgcaagtagt tattccaatc attcctggag ggatcagtac tgcagctggt gtcctgatat
      421 ttggccctta tgctggattt atctataact atattggaat ctgcattggt tcgattatca
      481 tttttctgct agggcgtcgt tatggaaagc cattcatact ttccatgatt agcgataaga
      541 catataacaa atatattggc tggctggata atcaaaaccg ttttgagaag ctgtttgctc
      601 tagctatttt tcttccagtt gctcctgacg atgcgttatg tttgatggct ggattgacaa
      661 acatgtcagt gaaacgatat acactaatca ttttgattgc taaaccactg tcgattttct
      721 tatatagtat ggccttgatt tatggaggtc aatatctaag tggtcttctt ggataatatt
      781 tattttgtaa aattagtcat tgattgacaa tgttaaatct gggaaagggt ctgcaaaaca
      841 cactgcaccc tttccttttt cattagattt gcgctacaat gtgaagagca ggtgatagga
      901 tgaattattt gaaacgtagc ttaggtaatg cttgggactt tttaaaagga acacaagctt
      961 atttccgtga tgtcttgctg atgcatggct ttattttatt tatttgttta cccttattga
     1021 gcagtacgac ccgttttatt ttacaccgag gggcaattga ctatttgtct tatgacaata
     1081 ttccttcaat tttttctcaa catcctgggg ttctactctc gttaatcgta gttttgatga
     1141 cgattttact gttagtttat ttcgaattta cttttctttt gatgagtgtt ttttttatta
     1201 aaaagaaaga accaatctct ttgaagcaac tcttacactt gacgatactt cagctgaaga
     1261 aagtgagacc cattactttt ttgtttttcc ttgcttattt tttattgatt ctaccgatca
     1321 gcggattaag ttttaattct gatctactgt caaaaataaa aatccccgct ttcattatgg
     1381 attttatttt tgctaatcgt tggatcatcg tcagttcctt tttactcgtt tatattttcc
     1441 ttggctatat tgggattcga ctgatttttg ctttaccgga aatgattcta cgggatcgtc
     1501 cttttcgagc agccattcgg gaaagctggt cattgaccaa atcgcgtctt ttggccatca
     1561 cgggccaatt tattgtgatt ggcggtacga ttttactcct ttcttcttta ggatatatcg
     1621 ttgttatttt ggcacaaagt atggttgaac aatttttccc agactatgca ttgatcagcg
     1681 ctgtgtttgc tatgacgctt ttacaaggga ttttattatt caatatcgtg atgtctaccg
     1741 tagggatttt ttatatcatt gttgatttca tggatgacga aggcttcttg cctgagatcc
     1801 ctaagtggtt tatcccacaa gcccccaact tgcgtttctc tgcgctgaaa aacactggtt
     1861 tgactctttt tgcagttttt ttcggtattg gcgtctgtct ttacaatatg gattatctga
     1921 cttcagctgt tcagacaaaa ccagtgactg tctctcacag aggtgtgagt acccaaaatg
     1981 gggcgcaaaa tactttagaa gcacttgaaa aaacaagcag agactatcat cctgattatg
     2041 tagagatgga tgttcaggaa acaaaagatg gtcatttcgt agtgatgcat gatgcaaatc
     2101 ttcgtcactt aacaggggta aatggaacac cacaggatct tacattgaaa gaattgacaa
     2161 atcttacagt tactgaaaac ggagtgcaag cccctgtttg ttcttttgat tcttatttgg
     2221 aaaaagcaca taagctaaac caaaaattac ttattgagat caagacctct aaaaaagatt
     2281 cggatgacct tgtcgaacga tttgtccata aataccgcaa agttatctta gagaaccatc
     2341 atattttgca aagtttgacg taccaaaccg tttcagattt aaaagaagca gaaccaaact
     2401 tttatgtggg ctacatctta cctttcaata ttgttgggcc ccctgtgacg cctgctgatt
     2461 ttctgacaat ggaatatagt acgatcaacc gtaacttcat taattctgcc caccaagacg
     2521 gaaaagaagt atatgtatgg acaatcaacg attcagatgc tatgagtcgc atgatgtttt
     2581 atggtgtgga tggtatcata accgatgaga tgggactact aaatgaaagt gtgaaacaaa
     2641 cagatgaaga aatcacttat tcagataaac tgctaaattt tgttttggga gtcggctaat
     2701 caaaaatcgt taatgaaaaa agtctttata caacaaaaaa cgcataacct gccaccgcag
     2761 gtatatgcgt tttttgtttg acgctcataa tgtgtgctta actttctctt gattccttca
     2821 tcgcacgaac aatagctaaa atagcaggaa ttgcagagat cactgcaaga ataattccca
     2881 ttttccctga actttttctt tttggcatat cttctcgctc ccttctgtct ttatttatat
     2941 tataagggag ttcttgcgaa ttctcaaaaa tactgtttaa gaaaccaatt cagcaggtgc
     3001 gctaatatca cagaatttta cgagcatgcg atacttgctt tcattgataa gttgatagtc
     3061 tcgttggtca taactttcaa catacacgac agccgtgttg atatatgtac gttcaataca
     3121 gccgattagt tcatgagata ctccgatcgg tttgcatata taagatcttc ctacttctgc
     3181 ttttaccatt tcattccctc ttttctttat ttttattttg ttttttcttt attttataat
     3241 aacaaattac cttgtatttg aaaacgtttt tatccatttt tttatttttt attttgagta
     3301 tctttctgct ttttagataa ctatttttta acacgatctt agacgttgtg acaaaaacgt
     3361 ttgtttatat tcctatgttg ttcgtgcgtt tttttcttta tattgctaag ttatttataa
     3421 agaggtgagc atatgattta tattcaagca atagtatttg taaccttgat gattgggcta
     3481 cttatcggat taaaagaaaa tagctaaacc atttaagaaa aaata
//
LOCUS       DACEPW010000220         3499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEPW010000220 DACEPW010000000
VERSION     DACEPW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.226"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            54..506
                     /locus_tag="IW819_002522"
     CDS             54..506
                     /locus_tag="IW819_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245606.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="IW819_002523"
     CDS             520..1209
                     /locus_tag="IW819_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245607.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..>3499
                     /locus_tag="IW819_002524"
     CDS             1334..>3499
                     /locus_tag="IW819_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1245608.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLL"
BASE COUNT         1184 a          463 c          735 g         1117 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttatta
//
LOCUS       DACEPW010000221         3445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEPW010000221 DACEPW010000000
VERSION     DACEPW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.227"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            208..429
                     /locus_tag="IW819_002525"
     CDS             208..429
                     /locus_tag="IW819_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245609.1"
                     /translation="MKDNSIDVDPHVQMKTTGKLHQVTQDIENPDQYLLFGEETTNEV
                     TQFVLLQLKVDQEAQKIDAIKIYYVRSVY"
     gene            651..881
                     /locus_tag="IW819_002526"
     CDS             651..881
                     /locus_tag="IW819_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245610.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKVYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            895..1227
                     /locus_tag="IW819_002527"
     CDS             895..1227
                     /locus_tag="IW819_002527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245611.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRKEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLVWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            1271..1609
                     /locus_tag="IW819_002528"
     CDS             1271..1609
                     /locus_tag="IW819_002528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245612.1"
                     /translation="MKRIVKIFGILGVFLIGLSTFVSAGISVDASETNNFLVKQQETE
                     INIIQNNLATHKDSKGNRLVYIIDESDLKSSLDRINSEITVSEIQQYLNEFNRSIQKK
                     WPWMANRSAS"
     gene            1623..1817
                     /locus_tag="IW819_002529"
     CDS             1623..1817
                     /locus_tag="IW819_002529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245613.1"
                     /translation="MKDQKTNNKTGERSACSWAFTGAGLLHSGIYTAAALGLSLTGVG
                     AAVIPFAVGTAYGIAGAAIC"
     gene            2318..2725
                     /locus_tag="IW819_002530"
     CDS             2318..2725
                     /locus_tag="IW819_002530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245614.1"
                     /translation="MSRFFKFFSLLGVMLIGLSTLVGAGTSVYAAEKVNEVPDIETQR
                     QQAVESLKFYLEEAGYVDTETHQYIVTDLEAIQKKAELDGSEGIAGKFILENFVQPTG
                     KRQKRDLQKYAACVVMNSLPFGVLFGIYLIVKI"
     gene            2909..3264
                     /locus_tag="IW819_002531"
                     /pseudo
     CDS             2909..3264
                     /locus_tag="IW819_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1271 a          411 c          645 g         1118 t
ORIGIN      
        1 gaaacaatcg aagcagtaaa agaaacaagt ggaactcttc aaagtactga aagtaacagt
       61 caaattggtt cattagaaga cgagacagcg caacaaatag atgaaatgac aaaaaatttc
      121 ttaaaagatt ttgctagaaa atggagtaat ttcacagatg tttataaacg caatcaagct
      181 gtgagggact atatgacgga tcgttgtatt aaagacaata gtattgatgt agacccgcat
      241 gtacaaatga aaacaactgg gaaacttcac caggtgacac aagatataga gaatccggat
      301 caatatttgt tatttggaga agaaacaacg aatgaggtca ctcaatttgt attacttcaa
      361 ttgaaagtag atcaagaagc tcaaaagatt gatgcgatca agatttatta tgtacgaagt
      421 gtgtattaaa ataaaaaatg aacactcata tttattatgc accgaaaaaa tagaaaaaaa
      481 cagactgttt ttttaagaag aagtatgtca ttctttactt gaaaaaagtt gttataggac
      541 agaaaggata aaacaaaaac ggaaaaaata tgtcttatga ataactacaa gaactattct
      601 attttttagg agatggatac gagatgaaaa aattaattgg ctatagtgtg atgtgtattg
      661 gttttgttgg tgtgggattg tttgtatcga ctgaatctgc ttctgcggct gaatggtata
      721 atcctatctg gtataatccg aacattgaat gtcaaaaagt ctatccgtat ggaccgacaa
      781 atctgaaaaa tggaaaatgt agagtaaatt ggaatggcgt tgtagatagt gtaattactg
      841 gttcaatgga ctctgctcta catggcttta cgggtcacta gaaggaggaa gagaatgtct
      901 gaagagaaaa aagaaatcgt tgaatcatta gtagctttaa aagattctct ttctaagatt
      961 gaatatgttg ataggaaaga aattcaaaaa ctaattgatg atactattat tgaaatccaa
     1021 gatgcgcgtt gtgaaggtat aaaaatttct gtagctttat ctaaagtaat agaaaaaatg
     1081 aaccgttcat tagcgtttaa tggattgaag ctagatagac aaactagtct ggtatgggat
     1141 catttaaaag acttatatga caagtctaaa agaagtgaac ggactgctgt atcaatctta
     1201 aaaggccttt gggggatgaa ttcataatca agatatttta aaaataactt atttttaagt
     1261 aggaaaaatt atgaaaagaa tagttaaaat atttggtatt ttaggtgttt tcttaatagg
     1321 tttaagtact tttgttagcg ctggaataag tgtcgatgct tctgaaacaa ataatttttt
     1381 agtaaaacaa caagaaactg aaataaatat cattcaaaat aatttagcca cacataaaga
     1441 ttctaaagga aatcgattag tatatattat tgatgagagt gatttaaaat caagtttaga
     1501 tagaattaat tctgaaatta cagtttctga aatccaacag tatctaaatg aatttaatcg
     1561 ttctattcaa aaaaaatggc catggatggc taaccgatca gcttcatgaa gttgagaatt
     1621 atatgaaaga tcaaaaaaca aataataaaa ctggtgaacg aagtgcctgc agttgggctt
     1681 tcactggggc tggacttcta cattctggga tttatacagc tgctgctcta ggtttaagcc
     1741 ttacgggagt aggtgcagcc gttattccat ttgctgtagg aacagcctat ggaatcgcag
     1801 gagctgcgat ctgttaatat gaaaaaaaac aatatcaaat agttcatttt ttaattactt
     1861 tattatttag cccacttttt tatagaagtt tagataaatc agtagtcttt acgatgctag
     1921 ttattttact tttaaatagt acgagtttct attttttgaa aaactatcat ggtagaaaaa
     1981 aactttttcc tttatttcaa tttatttggg atatcagtgt gattttagtt tttttctggt
     2041 tatttaaata gtcacttaag aaaaaggaag aagcaaattt gctatcttca tttgaatgat
     2101 gttggtactg tttcgctatg taggtaacaa tacaatttta ttatataccg aaaaaataga
     2161 ctgtttttta agaagaatta tatcgttctt tacttgaaaa aagctgttat aagacagaaa
     2221 gaataaaata gaaacggaag gaacgtaaaa acgtcttatt gaacagctta aaaggataaa
     2281 aattgataat caacaatact tttcaggagg gataaaaatg agtcgttttt ttaagttttt
     2341 ttcattatta ggtgtaatgt taattggatt aagtacgtta gtcggtgccg gaaccagtgt
     2401 ttatgctgct gaaaaagtga atgaagttcc tgatatagaa acacaaagac aacaggcagt
     2461 agagagtttg aaattctatt tagaagaagc tggttatgtt gatacagaaa cacatcaata
     2521 tatagttact gatttagaag ctatacagaa aaaagctgaa ttagatggct ctgaaggtat
     2581 agcagggaaa tttattttag aaaattttgt gcagccaaca ggaaagcgtc aaaaacgtga
     2641 tcttcaaaag tatgctgcat gtgttgttat gaactcatta ccatttggtg tgttgtttgg
     2701 gatatactta atcgtgaaaa tctaatgcaa actttaatta atgcgttgca agggaaaaat
     2761 tatgattatg ctgttaaaat acttttagat gtagcaaaaa gaagtttaac tcctgcacaa
     2821 tttgcaaaat tcaatgctgc aactattgca gctggtatag ctattaatgc tatttcttgt
     2881 tgggggaagt aattagtgga gtgattatat gagtatagat gattgtttag aagaacaaat
     2941 aagattattt ttaaagtttt taaaagttaa agttcaaaat aactacttca catgttatag
     3001 ttatgcacac cctgttttaa atagtcttcc ttttaattct ttagtgcaag gattgattac
     3061 aaatattgtt ttattagtct gtacaccaaa tgaaatattt gtaaaaaaga taggcagtgg
     3121 tatttcttca gtaagattaa aagagaaaga atatactcaa aagattattc atattcctaa
     3181 gcctgaaatt aagcgttttg agataaaaga ctattctgct ttaggactgt tctcaattgg
     3241 ttactggtga taaaaaaaga atactaaaac tatacgtaga atataagtaa tcggcatcaa
     3301 tcctaattat gtgattgaat gccgattttt gttacgcgct atttttaatt atctgtaaga
     3361 agaataattt tattttactc atattcgttt tcactttgaa gaagtaaatt gcaatttctt
     3421 ataagttaag cagctatgca aaagg
//
LOCUS       DACEPW010000222         3420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEPW010000222 DACEPW010000000
VERSION     DACEPW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.228"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..924
                     /locus_tag="IW819_002532"
     CDS             <1..924
                     /locus_tag="IW819_002532"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAQ1245615.1"
                     /translation="STNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPPIQNGNK
                     LNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIARERKQVF
                     HNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVMKDGLYH
                     QNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETIPYYTSA
                     YDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLNILRHYA
                     FHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            974..1939
                     /gene="nrdF"
                     /locus_tag="IW819_002533"
     CDS             974..1939
                     /gene="nrdF"
                     /locus_tag="IW819_002533"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1245616.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(2028..3167)
                     /locus_tag="IW819_002534"
     CDS             complement(2028..3167)
                     /locus_tag="IW819_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1245617.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
BASE COUNT         1214 a          641 c          591 g          974 t
ORIGIN      
        1 tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt gcgtacgatg
       61 actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc accgatccaa
      121 aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca cacttatctt
      181 gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa tatttacttc
      241 atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga aagaaaacaa
      301 gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga aaaatacgta
      361 actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg catttttatc
      421 ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg actgtatcat
      481 caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga tacgagtgca
      541 agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat cggtaaaatt
      601 tattacccag cgccttatct atccaatgag acgattccgt attacacttc tgcttatgat
      661 atggacatgc gtaaagtaat cgatgtttat gcaatggcac aacaacacgt cgatcaaggc
      721 atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga atggaagaca
      781 acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt ccacaaagga
      841 atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat cggaagcaat
      901 caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat attttaatta
      961 aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg aaattgaaga
     1021 tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag atacacgtat
     1081 cccactttca aatgacctag atgattggcg tacattgtct caattggaaa aagatacagt
     1141 aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa gtggcatgga
     1201 tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca atattcaatt
     1261 catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga atacgaaaaa
     1321 agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa aaaaagctga
     1381 gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg ccagcgtctt
     1441 tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt taggaaacaa
     1501 caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat ctgtacatgg
     1561 aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag cagaacaaga
     1621 aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg aagaacgtta
     1681 tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat tcctacgcta
     1741 taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag atactgccaa
     1801 tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc atgacttctt
     1861 ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag acgacgatta
     1921 tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa aataaaaaga
     1981 gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta ttcacttgtt
     2041 tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct tctcaataaa
     2101 tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg tatgcaaatc
     2161 agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat gatcgtcaaa
     2221 ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc atctaattgt
     2281 tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat aaacaaaccg
     2341 acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat attcgtgatt
     2401 ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat tgccaaaaag
     2461 ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc agataatgtt
     2521 tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg aaagaagtga
     2581 gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat aaatggaacg
     2641 gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac acttgcagat
     2701 aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga tgctaattgt
     2761 tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa ccatttgctt
     2821 gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc cattaactga
     2881 ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa cgtcaataaa
     2941 aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg atcgaacata
     3001 taatagataa aaccagcaac aagcaatggt aaaaaaactg ccgaaagcat tttcaatatt
     3061 ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt gagtactagc
     3121 tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca tctcctccaa
     3181 ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag attttcattt
     3241 gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac ttaaacaaaa
     3301 acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa taaacggtgt
     3361 tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg aactattttt
//
LOCUS       DACEPW010000223         3418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEPW010000223 DACEPW010000000
VERSION     DACEPW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.229"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..751
                     /locus_tag="IW819_002535"
     CDS             68..751
                     /locus_tag="IW819_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1245618.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="IW819_002536"
     CDS             753..1589
                     /locus_tag="IW819_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1245619.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="IW819_002537"
     CDS             1600..3354
                     /locus_tag="IW819_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245620.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         1072 a          689 c          646 g         1011 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctc
//
LOCUS       DACEPW010000224         3354 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEPW010000224 DACEPW010000000
VERSION     DACEPW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.230"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            29..388
                     /locus_tag="IW819_002538"
     CDS             29..388
                     /locus_tag="IW819_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245621.1"
                     /translation="MEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDE
                     RTLLLIEDCYYLWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFRE
                     KEFSNQKKKIDKKLKKR"
     gene            complement(559..900)
                     /locus_tag="IW819_002539"
     CDS             complement(559..900)
                     /locus_tag="IW819_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1245622.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            986..1693
                     /locus_tag="IW819_002540"
     CDS             986..1693
                     /locus_tag="IW819_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1245623.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            1680..2003
                     /locus_tag="IW819_002541"
     CDS             1680..2003
                     /locus_tag="IW819_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1245624.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2092..3129)
                     /locus_tag="IW819_002542"
     CDS             complement(2092..3129)
                     /locus_tag="IW819_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1245625.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT          955 a          702 c          606 g         1091 t
ORIGIN      
        1 caaaaacagg aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt
       61 tgaatcgctt attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat
      121 tgctactctc gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga
      181 ggactgttat tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca
      241 aatgttctct tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta
      301 ctccataaaa atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa
      361 aatagataaa aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa atagcaagta
      421 aacgataaca accatcctaa atccacaaga aatacagcag accttgcttt aaaaacacga
      481 atcatgcaaa ggatgaagta aagaagactt aacgcgtacc caatattagt catttgacta
      541 ttaggtacgc gttttgtttt ataagtcatg acaaatttcc ttcgttgctg gatagatttt
      601 ccggcattgt tgatagattt tatgatactt ttctacattt tgtaagagag gttccactgc
      661 atctgtatat tggataaaat tctgacaaca actttcaggt gtgtcgaacc accctaaccc
      721 tagtgcagca atcattgttg cgcctaatgc aggtccttcc tccacactta aacaatagac
      781 cgttgcatcg aaaatatcag cttggatttg tagccatgca agatttttcg cgcctcctcc
      841 caccgaaaca atacgtgtaa atctgcgacc cgctagttct ttacgtttca atcccaccat
      901 tgacaaatgc tcattaaaaa tttagaatat tcacaagttt aacgacaatt atgaaaattt
      961 atgtcgtgct aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa
     1021 agacacgtta cctaccgttt tcggttatat cggtattgga cttgcatttg gtatcgttgg
     1081 gaaagctgcc ggatttcatc cactagtcgt cacgttgatg tccctgctgg tctatgctgg
     1141 ttctgcccaa tttatcacag tcagcatgct tgctagtcac agcccattgc tttccatcgt
     1201 tttctcgacc tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta
     1261 tttcaagaaa aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag
     1321 tttcgcactg ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat
     1381 gaatacagct aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact
     1441 tttagggaat tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat
     1501 gtttatcggg ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca
     1561 gttgatcctg attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag
     1621 caacttagtg attgtagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca
     1681 tgccttcttt tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt
     1741 ctagggtcct accatttgtc ttactgaaaa aattcgattt accgcaaccg ttgcttgagt
     1801 atttaagttt tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc
     1861 aaaatatcgg acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac
     1921 ttttagctgc tattctttca aaaagcttat tggtgatcgt cttggcagga attctttccc
     1981 tttcgttgat tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg
     2041 cgctcttgtt tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt
     2101 gaacggacca ctaacaacga cttttcctaa catatgtcct gtttctacgt ctttggttgc
     2161 ttgtttcaag ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat
     2221 aatcccttca tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc
     2281 atagttatag tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt
     2341 ccaaacacct atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg
     2401 tcggacaagg tgcttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata
     2461 ctctacttga tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat
     2521 tggatagtct acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta
     2581 gacttctagg ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccggatcc
     2641 gttgatcact aagatttttt tcccgcgatt ggcattttct tctggagtta gaccaaattt
     2701 ttcaaataac aattcataag cggtcaatga ggtcaacggc aaggctgcag cttcttcatc
     2761 tgaaagattt tttggtgcaa gagcgacaat acgttcgtca actagttggt attcttgatt
     2821 gcttcctgct cttgttgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg
     2881 cacttcgtca cctacagcaa caactttccc gactccatca aaacctaaga tacgtaaagc
     2941 atttcttatc ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt
     3001 gacttttacc agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa
     3061 gctttttgga tcatcgatcg gcaacccttc ataaaaaccg actaccttca ttaattcctt
     3121 ttctgacata cgaattcctc cttagcttca tcaagttgga gcatccatac agtgttcatt
     3181 tcaatatata tagtatgcta tttgttccac cttttttatt catcataatt attctcttac
     3241 ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag acttttgtca
     3301 caacctctta taaacggtgt tctcctacca actcttcggt cacaagtcaa aata
//
LOCUS       DACEPW010000225         3346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEPW010000225 DACEPW010000000
VERSION     DACEPW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.231"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     regulatory      227..271
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            295..1269
                     /locus_tag="IW819_002543"
     CDS             295..1269
                     /locus_tag="IW819_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1245626.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            1274..1849
                     /locus_tag="IW819_002544"
     CDS             1274..1849
                     /locus_tag="IW819_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1245627.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(1916..3055)
                     /locus_tag="IW819_002545"
     CDS             complement(1916..3055)
                     /locus_tag="IW819_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1245628.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          946 a          660 c          647 g         1093 t
ORIGIN      
        1 aaaaataacc atcgtacacc cgccgctttc tgccaaacta taatctattt attatacaat
       61 ataccgatca ataggattct ttttcttcgg cacacgtaaa ttccatttct ttttcattgt
      121 atcatttact taaagttctt ctcaatagat ttacttgtaa gagagcattt acattcacaa
      181 taaaagccag tatactaatt tcgtgcatta taaacaagac actcgtcttg gactggttcg
      241 gaaacttccc agaataaaaa actaagtatc tctaaagaaa ggagattgga aataatgcgt
      301 aaagtaatta ttgattgcga tccagggatc gacgacactt tagctttatc actagctgtc
      361 aaatcccctg atatcgaagt agttgctatt accgtcgttt gcgggaatgt ccctgtagat
      421 atcggtacgc agaatgtgct gaaatgtttg gagcgctgtg atcgtttaga tatccctgtt
      481 tatcaaggaa tggaaaaacc tttgaagcaa cctttcatta gtgcgcaaga cactcatggg
      541 ttggatggat taggggacac aaatttcccg atggtcttag ccaaacaggc agaatcgctc
      601 catgcagttg attttctgac tgattatttc aaagaaaaaa cagatacatc agtcattgct
      661 ttggggcctt tgaccaatat cgcttctgct ttgaaaaaca gcccatctgt tgggaaacat
      721 atggatcgtt ttgtctcaat gggcggaact tacaaaagcc atggtaactg ttcaccagtt
      781 gctgaataca attactggtg tgatcctgaa gcagcttctt atgtattcga aaagctcaaa
      841 caaccgatag aaatggttgg gttagatgtg acaagagaaa tcgtcttgac accgactatc
      901 cttgaatatt gctgccaaat gaatcctgaa gagggagagt atctaaaagc gatcactcgt
      961 ttttattttg actttcactg gaaacaagaa agaatcttag gatgtgtcat caatgatcca
     1021 ttagctgttg cttattttat cgatgagtcc atctgtgaag gctttaaaag cttcactgct
     1081 gtagaaactc aaggaataag tcgcggacaa acattggttg atcgttatga attttggcag
     1141 aaacctgcta acagtaaaat tatgacaaaa gtagatactc gtcttttttt ccgaaagttt
     1201 ttaaccgttc ttttaaatgc acaagaagaa acgattatga aagatttgga aaggttaaag
     1261 atgggctaac aaaatgaata aaaacaaaat ggcaactcgc gtacttacag tgatggcttt
     1321 gtgtattggg atcaattata ttggaggaac gattgcttta tggcttcgtt tgccaatcta
     1381 tctcgattct atcggaacga tttttgctgg tgcattgctt gggcctgtac ctggaatgct
     1441 gacaggattg tccagcggta ttttaagtgg agtgactaca gatatttttt ctctgtatta
     1501 ttcgccaatc caaattctca caggcttgct tgctggattg attttgccaa gatatctcgg
     1561 caaagataaa aacagcttga aactgttatt actcgctttg attttgtcgg tacctggaac
     1621 gatcgtttct tcgatcatta ccgttcagtt atttggcggg atcacctcct caggatccag
     1681 tatgatcgtt cagctattac atgggttagg tttaagtcag acaacaagtg tgatcttggt
     1741 acaggtgggt acagactacc ttgatcgctt gttgtctatc gtcgttgttt cattagtcat
     1801 cactatgttg ccccaacgct tgatatattc aaaaagaaaa ataggctgaa taaacagagc
     1861 tggattagca aaataatgct aatctggctc tgttttttca tttattaaaa aaatcttacg
     1921 ctaagtagga taaaataatt ccttgatatt tttcgatcat atccaaataa ttatcgattt
     1981 caacatactc atctacttga tgaggcaaag taacgacacc aggcccaaag acgacaaagt
     2041 cgaaggaatg gttagctttc gtaaattcgg ctgcatccgt tgttgctgcc gctccaacta
     2101 aaggtaatgg ctggctgaat tgttgctgaa tggaatgaat caaaggagaa tctggatcag
     2161 ctttgacagg tattttgttg taatcgatca tcaattccaa atgatagtct gtctcttgat
     2221 tcaattcatt gacgattgat tgcagcaatg caatgatttt atcatttggg tattctggaa
     2281 tcgatcgaat gttcccttgg agctgcgcat ggcttgggat gctgttgact tgattcccgc
     2341 cactgatcaa tgtaacatta tggattgttt ttcctaggac aggattctcg attgtttcag
     2401 cgaggtgatt catttcagca tttgctttcg taatgaattc atttaggtga ttgattgcat
     2461 tataaccttg gtctggcata gagctatgcg cttcttttcc gtgcgaggtg actgtatagt
     2521 tgattgatcc catgtgcgta tacatcaaac tgtaattggt tggctctccg atgatcaagg
     2581 catctaagtc atctacatag cctgctttcg tgagctgttc cccacctagt tcacccactt
     2641 cttctccgac agtggctaat aatttgactg ttccgttaaa tggttttcct gactctttca
     2701 gttcgatcat ggcaatcacc attgctgcca agccactctt catatctgta gcaccacgtc
     2761 cgtacaaacg gttcccatgg atttctgctg caaagggtgc ataggtccaa gacgattcat
     2821 caccagccgc cacgacatcc atatgtccag ataggcctaa cacttttcct gattggccct
     2881 tttggtaacg agcaatcaaa ttatctctac catctctgta agaaacaatc tctcctgtta
     2941 tgtcatgcct tgccaaaagc ttattcaaat aggctgctac ttcaccttcg tttccattca
     3001 ctgatttaat tcggataatc tcttgtaaaa tcgcgatttt ttcttctttt ttcatctaat
     3061 cctctccctt gccatttctt tttattgtat ttgagtgtgc tcgaattttc aaataggagc
     3121 aaccaaagta aaaatgaaga aagagagcca ctttatgaaa aatatgctgc tacttccctc
     3181 ctattttatt tatacgagag gacttgccca tagattccta ttctactgtt ctatccttaa
     3241 aagctcttat atgatgctag ttattgttag ctttcctttt tcaaggatga tcttttcttg
     3301 taccattctt tgtaaacatc atatacagaa gctagttgca aaaaaa
//
LOCUS       DACEPW010000226         3273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEPW010000226 DACEPW010000000
VERSION     DACEPW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.232"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..718
                     /locus_tag="IW819_002546"
     CDS             <1..718
                     /locus_tag="IW819_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1245629.1"
                     /translation="DQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRLVKVY
                     QTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFI
                     GTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILA
                     VGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGF
                     VLLMITNTIARRFDSDSALF"
     gene            730..1650
                     /locus_tag="IW819_002547"
     CDS             730..1650
                     /locus_tag="IW819_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1245630.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1691..3148
                     /locus_tag="IW819_002548"
     CDS             1691..3148
                     /locus_tag="IW819_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1245631.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
BASE COUNT         1055 a          572 c          664 g          982 t
ORIGIN      
        1 ggatcaagca tttctcatca ctaaaaatac aattttatat aatgtagcat ttatcctttt
       61 gaatctgctg atctcagtcg tatttgcaat cattatgagc gagttgagaa ataaacgact
      121 cgttaaagtt tatcaaacga tgtccttact accttatttt ctatcttggg tcattatcag
      181 ttactttgtt tacgcttttt taagtcctga caaaggaata ttcaatcagt ggatcactgg
      241 acatggagga gaagctgtca attggtataa tgaaccgaaa tattggccgt ttatcttggt
      301 gttcattggt acatggaaag ggattggtta taacagcatt atttactttg catctgttat
      361 gggaatcgat ccgacttatt atgaagctgc tatggttgat ggagctagca aatggcagca
      421 gatcaaacat gtgacgatac cacaacttat tccactaatg acgattttaa cgattttagc
      481 cgtaggaaac attttccgag ctgattttgg cctgttttat aacattccta ggaattccgg
      541 tgcattatat gatgtgactc aagttttgga tacgtatatc tacaatggcc tgacatccac
      601 aggagatttt ggtatgacgg ctgctgcagg actttaccaa tcagttgtcg gctttgtctt
      661 gttgatgatc acaaatacga tcgcccgacg ttttgatagc gattctgcat tattctaagg
      721 aggaaaaaaa tgaaaaaaaa gaaagtggag agagtccaag tccgctcttt caataagacg
      781 gctaattttt tctctaatat aatgattgca ctctttgcta tttcctgtat tttgccattt
      841 atatttgtta tcgccatctc ttttaccgat gagtccatat tgtcgactca cgggtacagt
      901 ttttggccag aaaagttcag tatatttggt tatacgtact tgttcgacca gatgcaggat
      961 aaaattttcc aagcgctttt cgtcactgta cttgttactg ttttaggtac actgatcaat
     1021 agcacggcta cttctttata tgcttatgcg atttcacggt caaactttcc attccgtcgt
     1081 ttttttaccg ttttttgttt gattacgatg ttgttctcac cagggatggt ggccaattat
     1141 cttgttatga caaatcttct tcagttaaaa gatacgattt gggcattgat cttgccaatg
     1201 gctgtcagtc ccttcaatat tattgtgatg cgcacattct ttaaacgttc ggtctctgat
     1261 tcgattattg aatcggcacg gatcgatgga gctagcgaac tgcgaatatt tgttcagatc
     1321 gttttaccgt tagctgttcc aggtattgcg acaatcagtt tatttgccgc attgggttac
     1381 tggaatgact ggttcaatgc tttactatac atccaagatg ataagctagt tccactacag
     1441 tatcttttga tgaaaatcca aagtaatatt caatatctga cacaaaattc aggagcagga
     1501 agccaattga caggcggatt agcctcagtt ccaggagaat ctgccagaat ggcgatcgta
     1561 gtcatctcga cgttgcctat tgcgatcagc tatccatttt tccagaagca ttttgtcaaa
     1621 ggtctaacaa tcggcggagt aaaagaataa agaaacaaaa atataaatca taaaattagg
     1681 aggaagaaaa atgaagttat ggaagaaact agcctttaca ggtgtttcag cgttgttgtt
     1741 gggaacatta gcagcatgcg gaggttctgg ttctaaagac caggcagaag ctagcaattc
     1801 ggacactttg cagatgtatc aaatcggaga taaaccagat aatttcgatc aattgatgga
     1861 agtagcaaac aaacgaatcg aaaaagagat cggtgtaaaa gtcaatatca attacattgg
     1921 ctggggcgat tacgaaaaga aaatgaacgt catcatttct tccggggaaa attatgatat
     1981 cgcctttgcg aacaactatg tttccaatgc ccaaaaagga gcctttgctg atttgactga
     2041 attagcgcct aagtacgctg aaaaagctta taatgattta gatgaagcat atattaaagg
     2101 aaatctggtc aatggaaaat tatatgcttt cccagtcaat ggaaatgtct ttgctcaaca
     2161 agtacttact tttaacaaag ctttactaga taaatacgat ctttcaattg atggaatcca
     2221 atcttacgct gatgcagaaa aagtcttgaa ggaattccat gaaaaagaac caaacacggc
     2281 agcttttgcc attggacaag gcttcaaagt ccaagggaac tttgattatc ctttagggaa
     2341 tacgttacca tttgctattg atctggatgg agatgattcg aaaatcatca accaatacga
     2401 taatgaaacg tatatcggac tgttacgtac aatgcatagc tggtacaaac aaggactcat
     2461 tccaagtgac gctgcaacga gcaataagga ttatccacta gaaggaaata cttggctaat
     2521 gcgtggagaa acacaaggac cgtatgatta tggcgataca attttgacaa atgcagcaaa
     2581 acaagaactt gtctcaaaag caattactgt tccactaaaa tctacagctc aagcacaaat
     2641 ggcaaatttt gtcgtatcaa atacttcaaa aaataaagaa aaatcagtag aacttctagg
     2701 gctgcttaac agtgatccag aattattgaa tgggcttgtt tgggggatcg agggagaagc
     2761 atgggaaaaa gtcgatggtt cagatgataa aatcaaattg ctagatggtt atcagccaaa
     2821 tacacatatg tctgcttgga atacaggaaa caacaagatt ctatatacac aagagtctat
     2881 tacggatgac atgattgcca aacgtgatca atcgattgct gatgcagaaa cttcaccgat
     2941 tttaggattc agcttcaaca ctgacagtgt caaaacagaa ttaagtaata tttctaatgt
     3001 catgaatcaa tatttagatg gattgaatac agggactgtt gatccagatg aaacattacc
     3061 gaaattgaaa gacgctttgg acaaggctgg ctatgataag gtattgaaag aaatgcaaaa
     3121 acaatatgat gagttccgac aagaataata atgaataaag acaaaggggt tgtgacaaaa
     3181 gacgtgtcac aacctcttgt tccgaataaa cggtgttcaa aagccagttt ctcggtatta
     3241 agctaaatat atgtaaaaat gtggaaagcc ata
//
LOCUS       DACEPW010000227         3233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEPW010000227 DACEPW010000000
VERSION     DACEPW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.233"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            596..1924
                     /locus_tag="IW819_002549"
     CDS             596..1924
                     /locus_tag="IW819_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1245632.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2126..2959
                     /locus_tag="IW819_002550"
     CDS             2126..2959
                     /locus_tag="IW819_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1245633.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2975..>3233
                     /locus_tag="IW819_002551"
     CDS             2975..>3233
                     /locus_tag="IW819_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1245634.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNK"
BASE COUNT         1068 a          526 c          668 g          971 t
ORIGIN      
        1 aaataaagct tatgcaaaga aaaagaaatc caatcacttg ggtttctttt tcttttatta
       61 ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac gaaagagtag
      121 agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa taaatcagca
      181 ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc ccaaagttat
      241 ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc aggtttttca
      301 ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt attttgaaag
      361 ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc attttcgtta
      421 tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac atttttcttc
      481 atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga gattgtattt
      541 ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg attggatgaa
      601 agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa atatcaaaca
      661 gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg aaaatgtttc
      721 agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga aagtggctaa
      781 tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc gtcataagat
      841 gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt ttttctggga
      901 taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa aagaggcaat
      961 cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg gccaatggga
     1021 tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga tgccggaaag
     1081 tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac tgcgtaaaga
     1141 tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta ttgaagaaaa
     1201 gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag gaacacctcc
     1261 aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg accgaggact
     1321 gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt atgttagcgt
     1381 gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag agatgctagg
     1441 aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa ctttcaaaaa
     1501 attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg atgtaggtca
     1561 atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg atgatctatt
     1621 tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa gtctgatgac
     1681 ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa aatggaaagt
     1741 cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga gtgatgcctg
     1801 gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga aagagcaatt
     1861 aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg gtgctttagc
     1921 atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg cttaagccag
     1981 ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta taattctaaa
     2041 taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac gaagtagaat
     2101 aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg atctgaaatt
     2161 aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc gtataaaaac
     2221 cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat tttttgaagc
     2281 agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata cgtatatgct
     2341 aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg gaaaagggga
     2401 tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc cacaatttgg
     2461 ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg aagaagattg
     2521 gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag aagaaacgaa
     2581 tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg tattgaaatc
     2641 ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga gtggtaatgt
     2701 gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt atgggaatcg
     2761 ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat ttttagatgc
     2821 ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga tttaccagtt
     2881 atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc aagtagatca
     2941 attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata ccaaaaagca
     3001 tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt cgatgttagg
     3061 agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca gtgaattcgt
     3121 caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta atcgtctccc
     3181 tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata agt
//
LOCUS       DACEPW010000228         3224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEPW010000228 DACEPW010000000
VERSION     DACEPW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.234"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(550..2163)
                     /locus_tag="IW819_002552"
     CDS             complement(550..2163)
                     /locus_tag="IW819_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1245635.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2304..3212)
                     /locus_tag="IW819_002553"
     CDS             complement(2304..3212)
                     /locus_tag="IW819_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1245636.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1038 a          556 c          571 g         1059 t
ORIGIN      
        1 aaataaatag gaacaaaaaa attaaattat ttaatacaca cattagggat cattcattga
       61 aggattagaa gacaacgagg atggcgaaaa atatgattac aatagaaaag tcgttctaaa
      121 ccaatcagtt cctagtatca cacaacgcaa gccagtcgat ttggtgagat tagaggagtg
      181 aaagcaaact ttattatata agaaaaaata ttcgaaaggg atgctagttt aggtactagt
      241 gctgatagat taatatgatg cctcgtaata ttaggcgtag ttactctaga gacatcacta
      301 tggactatga tttgtctcag agtcacaaca tgttttttta tgaaaagaaa cagctaaaat
      361 aattaaattg cgcaatgttt aaactttctt aaactataac ctaataaatg tgaagaaata
      421 atgcacaatt tcttcacatt tatcttctat tctatataca taccaaccaa caaccctata
      481 tactttttca tttttactcc tcaagtaaaa ataactccta gaccgcggct acccaccgcg
      541 gtcgtttttt tattcttcaa tcaaaatcgc tttgataccc aaacctgcca agattcgggc
      601 agaagtataa gtattcaatc ctcttctgaa agtaatgtaa acttttttat cttttgggat
      661 ttcattgatt cgatctcgta actcattcat tggaatattt tttgttgccg agatactgcc
      721 agaaggtgct ttattaggtt cacgtatatc taaaaagaac gcttctttca aatcgttttc
      781 gggtatatca cttgctttga tcgtttctac gatattcgtc atttgattga ttgcgacata
      841 accagctata ttcaacgggt cacgggtagt agaatacggt ggagagtaag gcaactccaa
      901 atcaggcaaa tcaaagactg tcaagtttcc ggtgattgct acagaaagtt ctcccatccg
      961 tttatcgaca cctttttctc caaatgcttg tccacctaaa atacgaccgc tatctgcatc
     1021 aaaaatcagc tttagattta atcttgtagc ttctggatag aaataggcat ggtcaaaggg
     1081 agtgataaaa acagttttat aatttgttat gcctgatgct tttaattcat gttctgtcat
     1141 tcccgcataa cttgctgtat gatcaaagat ttttgcgaca cctgcaccaa tatatccgcg
     1201 atagcgcata ggcgtaccat ttaaaatatc tgccagcata tgtccttgac gattggccgc
     1261 actggaaagc atactttgga tcggctgacc agtcactaca ctagtcgttt cgataatatc
     1321 gccaatcgca tagatgtccg gcagattggt ttgtaactgg tcatcaacaa tgatctgacc
     1381 tgtatctgat aattgtatgc cggttgcttt caccacttca ttcttcggag aaacaccaac
     1441 agcaaaaatt agcatatcag cagaaaggac ggagccatct gataatacta tttctcgacc
     1501 tttatcacgg atctcagtaa ctcttgtttc taaatgaacg gctaatcctt ctttcagcaa
     1561 tttgtcataa actaaattag caatttctgg atcataaggg aaagcgactt gtggcaattg
     1621 atcgattaaa gtcacgtcta agccgcgatg cttgaaattc tcagctaatt ccagacccat
     1681 aacacctgca ccaagaatga cgacatgttg tggatttttt tcttcaagga acgatttgat
     1741 tcgatccgca tccgtcacac tacggaggac aaagccgttt tctgcttcaa aaactccggg
     1801 aatatccgga taatctggtc tagcaccaga agagatgatc aacttatcgt aatctgtttt
     1861 tatttcttca tttgtggata ggtctttaac tgttaatcgc ttagtagaag gatcgattgc
     1921 tgttacttcg tgtttagtaa agacatcgat gttgtttttt gttttcaata tttctggtgt
     1981 acgttcgatc agcgagtcac ggtcagtgat cacacctcct aaataataag gcaaagcaca
     2041 acttgcataa gaaatatttt ctccgcgttc atagataatg atttcgtgcg cttcattcaa
     2101 tcgacggaaa cgagtggcaa atgaaggacc gccagctaca ccgccaatga caacaacttt
     2161 cataaaaaca tctccttttc tacgttgatc gtactatgtt atggtaaaaa acagcaaata
     2221 attgtgccgg aaaattattg tttattttga atagacagaa aatatagaga aataatttgt
     2281 ggataaaacc taaaaatttt tttctatttt aaggatagat aatactttga aggggataac
     2341 cctgtatatt ttttaaaagc tgaagaaaaa tgatggatcg aatgaaatcc taatatatct
     2401 gcaatttctg tataggttaa ttcattttct agcacgagac gttttgcttt ttccattttt
     2461 agttctttaa aatagctaat aacacttttt ccattcactt ttctaaaatg tttttttaag
     2521 ctggctggac tgataaaaaa aatttgggct aattgttcaa gcaaaaaatt ttctgaaaga
     2581 tgttcttcta aatatcgtat gattggatca actaattcat ctgaataaag aatatgcttt
     2641 tttttaggaa tgactttctc atttgaaggt attgtcaata aagcaagttc ctgttgataa
     2701 tgccttggta aggaagacaa ttgaattttt tgcagacaat aagtaactaa taaggaaagc
     2761 aaatgaatgc tcatctgttg gcatagtatg aaaaaagaat tgatttcctg atcataggaa
     2821 atagctggct tactatgttt tagataatca attttgaact gttcattttt cagaaaagta
     2881 atgatagatg ttatttcttg atctatagaa aaagattgtc ccgtaatatg tgctaataat
     2941 tgctcatcaa aataaaactg gataatgata aaagtttgtg tttccttttg agtagaaaag
     3001 atttggactt cttgctgttt atcaatgaat aaaagggaag gattatctag ttctatagat
     3061 ttatgtgcaa attcagcata taaattagcg ctttcattta ttagtagtcg aaaataggga
     3121 gcagcagata aatgaagatg ggtatctatg gaataggtta gtgttttttt gacaagattg
     3181 gaggattggt tgtttgttaa caaaagaacc actttccttt ctct
//
LOCUS       DACEPW010000229         3193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEPW010000229 DACEPW010000000
VERSION     DACEPW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.235"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            307..597
                     /locus_tag="IW819_002554"
     CDS             307..597
                     /locus_tag="IW819_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245637.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            610..1635
                     /gene="sppA"
                     /locus_tag="IW819_002555"
     CDS             610..1635
                     /gene="sppA"
                     /locus_tag="IW819_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1245638.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            1638..2297
                     /locus_tag="IW819_002556"
     CDS             1638..2297
                     /locus_tag="IW819_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1245639.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            2454..>3193
                     /locus_tag="IW819_002557"
     CDS             2454..>3193
                     /locus_tag="IW819_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245640.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVL"
BASE COUNT         1058 a          532 c          708 g          895 t
ORIGIN      
        1 gagtgcagtc cctagggcca tccgccgaat ctttcgataa accctctcct cgtcatctgt
       61 ttacacagac ctggcgctat cctaaaaggt ataactatcc tcctttttgt tttttatctg
      121 gaaacattta aaatcataac cttttgcgaa tgttcttgtc aacaaaaaaa gaataaaaaa
      181 taaattggta tatatcaaat tctttgtcaa atgtcctttt tatgtcaaaa aataatgggt
      241 ttttatctat catctttttc aggagttcgg tacaatagaa agtaatcgga tagaggagga
      301 gtaaaaatgg attcaaaaat tgagatcatg acactgggaa tgttaaaaaa acaattgtcc
      361 aaattcgaag catcagctgg tgtatcagat gatacgaaaa tatttttaga tactggctgg
      421 gatagcatcc aagaaatcgc acctgatgcc ttggaagttg ctcaagctcg tgagtttacg
      481 gtagaagatg aattaacaaa agagtcgttt tccgggtatg cgcgggaaga aaaagcagag
      541 cggttcgaca cttctgagca gtcagaaact gtgatcgtca taaaaaactt gtattagaaa
      601 ggagaaagaa tgaataaaag acgttgggca gcagttctga ttgctgcagg attatttgtg
      661 gtttcgttag tttcagctag tttgactaag cctcaggaaa acgatcaaat tgagggattg
      721 aacagttggt tatatgggga tgaagaacta gctccaaatg tgttggaaga gggagacagt
      781 acgcaaaaaa tcgtaaaatt atcagtagat ggaacgattg ctgcgggagg gtcttcaggt
      841 ttatttgctt ctgaaggtta caatcataaa aattttatgg aacaactgaa ggcgattgaa
      901 gaagatccaa cagtcaaagg tatcttttta gaagtaaatt ctcctggtgg cggtgtctac
      961 gaaagtgcgg aaatcgctaa gaaattggat acgattcgta aagaacatga tatcccaatg
     1021 tatgtcagca tgaaaaacat ggcagctagc ggtggctact atatttctgc tcaagcagat
     1081 aaaatctttg caacagaaga aacagtgacc ggctcaattg gtgtgatcat gtcaggattg
     1141 aattattcag gacttctaga aaaactgggt gtggaagata caacagttaa aagcggagcg
     1201 ttgaaagata tgggttcagg aacgcgacca gagacaaaag aagaagaagc ggtgcttcaa
     1261 gcatatatcg acagcgcata tagtcgtttt gtagcaatcg tcagcgaggg acggaataaa
     1321 tctgaagaag aagtcaaaaa aattgctgat ggaaggattt atgatggtgt ccaagcaaaa
     1381 gaagtaggtt tagtcgatga attgggattt cctgaagatg ctttaaaagc tatacgaaag
     1441 gaacaacagc ttgaagatgc ggaactagta gaatattctt ccacgccatc tgggtttgct
     1501 agtacatggt taggaagtaa actagcagaa ttccagggat tgaagccttc tgagactagt
     1561 cagatcttat ccgttcttga gaatctaggg acagcggaat caccaaaagc aatgtattat
     1621 tacggaggtg agtaagcatg tcagagcttt caaatgaaaa taaggaacaa gaaaaagata
     1681 agcaacagca gcctgctgta ggacctgact ccaacttttc ccaaaaggta ctgagctctt
     1741 ttgaaagaga gcctttgacg cccgaagaaa tcaaaaagaa acaacgtaaa tggaaaaagt
     1801 atacggacac aagaaatgaa ccgccaaaag caaatgattt tcctaagtat ttctatgcag
     1861 gtttttttat ccgaatgttt gccttcttgg tcgatctttt gtgtatcgga gctattacgc
     1921 gtttaacact tggtattgcc aaaaatctag gttggcttaa tttttcagat tcttatctga
     1981 gtctctacgg attcacagcc ttacttattt atctagcgta ttttatttta ttgacgaaat
     2041 tcaatcatgg ccaaacgatt gggaaaatga tctttggtat tcgagtcatc agctttgatg
     2101 aagaagaact tagctggtca accgttttag ttagagaagg tgcttgccgg ttcattctaa
     2161 aatttccttt gctgatgtta gggtatcttc cagcagtctt taatcagaaa aagcaacatc
     2221 tgggcgattt tttcactaat acaagtgttg ttacgatcaa tctgatcaaa gcgttcaacc
     2281 aggaagtcaa cagctgatga gcaataaaaa gcagtaacag ttgaaagcac cttctaaaag
     2341 tcagatctaa aatctagctt ttagagggtg cttcagttat ttttaattgt catgcttttt
     2401 ttgttttgct actattgaac atgagctgaa tatgagcaag aggaggacac tgcgtgaagc
     2461 cgattgattt tccagaaaaa tatgagaatt taatgcgagt ggcacaacaa gcactagcaa
     2521 accagcaata tcagcaggca aaagaattat ttcaacgagc ttacgagttg aaagaaagct
     2581 ttgaggctaa tagcctgctt gttttttgtc tttacgagtt ggacgagaaa aaagaagcat
     2641 tgaagcaagc actcttgcat gaaaaacagt atcttcaaaa tgaggagttt gctgaatttt
     2701 attttgactt gttaatcagt acacaagatt ttttatatgc tagaaaattg atagcaagta
     2761 cagattttta tgaagctttc gagcagcggg tcattgaaaa aatccaattt gcagaagaat
     2821 tatccggaca aatggagagg cagaagatca aggcactcca caataaatcg gaggaacttc
     2881 cttcgttgga acctgcacgt cagcttgcgt ctattgatga gatcgaacag cttccttatc
     2941 atgagtttat ccaaacagcg agtaaactta ttgtatcgcc agaggtccat attttagcaa
     3001 gggcaaaatt actggagaca cttaggcaat tgaacgaatg ccatcctgtt ttctatttga
     3061 cgattgaaga aaagttagtc aaagtcatac caaaagatct gccaaagccg cagcagcagg
     3121 caagttacca gcaattatgt gtattttcag atcgttacgg gaatgaagat gcgctgctta
     3181 gttccgtgtt aaa
//
LOCUS       DACEPW010000230         3182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEPW010000230 DACEPW010000000
VERSION     DACEPW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.236"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..629)
                     /locus_tag="IW819_002558"
     CDS             complement(<1..629)
                     /locus_tag="IW819_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1245641.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEP"
     gene            complement(622..1890)
                     /locus_tag="IW819_002559"
     CDS             complement(622..1890)
                     /locus_tag="IW819_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1245642.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2142..2603
                     /gene="mscL"
                     /locus_tag="IW819_002560"
     CDS             2142..2603
                     /gene="mscL"
                     /locus_tag="IW819_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1245643.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(2662..3108)
                     /locus_tag="IW819_002561"
     CDS             complement(2662..3108)
                     /locus_tag="IW819_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1245644.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT          903 a          662 c          567 g         1050 t
ORIGIN      
        1 ggctccatct taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag
       61 cacgtataca agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg
      121 atatgcaatg ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta
      181 gggtcgtctt catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt
      241 tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc
      301 tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa
      361 gctccttgtt tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt
      421 tctaaaaaat gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata
      481 aattcgttat cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca
      541 tttttaggca acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca
      601 ttgatttgct catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc
      661 ctgcaattcg atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg
      721 tttcaaccat tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc
      781 ttcgattgcc gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt
      841 tgtctgagac agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg
      901 aatcagatga agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa
      961 tcctcggaac gtatccacgc tactagaggc ataataagct agactttctt tctctctgac
     1021 attcatgaaa agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc
     1081 aaaacgttct ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg
     1141 aacgattggc tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa
     1201 cagatctgga taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc
     1261 tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc
     1321 ttgatagtag gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa
     1381 ccctgggatc ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc
     1441 aaaagtttgt ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa
     1501 gtcaaacgtc aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt
     1561 taaaaaatct accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa
     1621 actgatccct gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct
     1681 tgcgccataa agctctgcaa gttgcgcact taactttgtc tgatccggat aattcaaact
     1741 atttgtttcc aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg
     1801 cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag
     1861 attcacgcct tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc
     1921 ccgcttactc ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta
     1981 taagttcagg caattcgccg ttttttctaa atgatgttac actttatagt atagcataga
     2041 tgttttttat caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt
     2101 ttcgatatac taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag
     2161 agttcatcat gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt
     2221 ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg
     2281 tcgcaatgat cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc
     2341 cgacaaaagg cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga
     2401 tcactggttt tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct
     2461 ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca
     2521 tacgcgatct tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa
     2581 atctttccga tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt
     2641 cggctttctt tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg
     2701 aatatcatct tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc
     2761 ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat
     2821 gttaatcgtt tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac
     2881 aaggacacgt ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat
     2941 cttcccatca gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt catcaacttc
     3001 ttgataagga cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta
     3061 aaactctgct gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc
     3121 ccctgctttt ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga
     3181 at
//
LOCUS       DACEPW010000231         3166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEPW010000231 DACEPW010000000
VERSION     DACEPW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.237"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            188..1588
                     /locus_tag="IW819_002562"
     CDS             188..1588
                     /locus_tag="IW819_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1245645.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHILTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKD
                     SRYVTKKEINKKNENK"
     gene            1575..>3166
                     /locus_tag="IW819_002563"
     CDS             1575..>3166
                     /locus_tag="IW819_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245646.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTA"
BASE COUNT         1070 a          470 c          532 g         1094 t
ORIGIN      
        1 aggtgattta tcttttcatc tttcacgaat caaagggtta tcttctattt ttgaagggcc
       61 aatcaactat acaacattta acaattatgg agatggactg aactattttt atccttttct
      121 tacaataatt cctgcagtcg ttttttatgg aatttcaaat aacttaatat taagttatgt
      181 tctatatatt tggttattaa atatttgtac gattctgatt agtttttatt atggagaaaa
      241 gttttttaaa aagatagatg ctgctttttt atttagttgt ttatatactt ttgcaactta
      301 tcgaacagtt gatatctatt atcgatccgc aattgcagaa gcaatcgctc ttacgattat
      361 cccagctgtt ttatattatg cttatggatt gatattcaat agagaaaaaa aagtcatcgc
      421 tttatctgtc agtataagtc tacttgtgta tactcatatc ttaactacac tattgtgtag
      481 tgcattgatt gcttgtatga ttatagttcg tttgttttta agcaagcaaa taaaaaacga
      541 tataaaattt gtggtcgtga atttagtaaa ggctgttttt ttgacttgtg cattaactag
      601 ctatttttgg tatcctatgt tgcagcagat gaattatcaa gaaatacaca gtcccttcaa
      661 aacagatttg cagctagaag cactttctat ttcagatagt atcattcaag cgttaaataa
      721 cgatattaca acatacacta tggggttatt aggattggtt gcgttgattt ttcctttatt
      781 catactgaaa gaaatggaga aaaaagaaaa aatcatctat gttggcgtag tttctacatg
      841 ggtatgcgta actaatttat ttccatggtt tttgtttcaa aaaacaccga taagcttact
      901 ccagtttcct tggcgtatct taggtatcca aatctttttt tcttcaatga tcataacgct
      961 tgtttttatg aaaaagacta tgaataaaaa atattcatgg ttaatcattg gcgctactgt
     1021 ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat tacgcaaaag ttatccaatc
     1081 aaaacacggt catattgtta tcaatgaaga aacactccca cgttatacaa catcagatat
     1141 gggaggatta tacgattacg ccccaacaga agcgataaaa gaaaggcctc acttagaaaa
     1201 acatgaagta aaagtaggag aatcgtggga aaaagggaag tataaagctg cagataataa
     1261 cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg acagtaccag tatatgctta
     1321 tttaggtaca caggtaaaag taaacggtaa agtcgtaaat gactcttata aaaaacatgg
     1381 cttagtgaat gtggatgtaa atgctggaga aaataagata gaaattacaa caaaatatac
     1441 tccaactgct ctcatggctt tatggatcag tattctgact tattgtttag tggtgtattc
     1501 aagttttaaa cgatttgcac ctaataagtt gaaagattct cgatatgtga ctaaaaagga
     1561 gataaacaag aagaatgaaa ataaataaaa aagtagtaat attgagtgga ttaattatag
     1621 ttagtagtat atattctttc atctttttca aaaatacaca gagtatttat accactggag
     1681 atctttcttt tcacttatct cgaatcaagg ggttatcgac tatttttacc agtccgatta
     1741 attatgagac attcaattat actggatatg gtgtaaatta tttttatccc tttctgactt
     1801 tttttccagc tgtaatgtta tattggatga caaaaaattt gattgtaagc tatattattt
     1861 acgtttggtt attgaacttg tgtacaacac tcgtagctta tcattatggc gagcggtttt
     1921 taaaacaaaa aaaagcggca tttttattta gttgtctcta tattttctct gcttatcgga
     1981 cagtagacat ctactatcgt tccgcaattg ccgaagctat cgcaataaca cttgttatcc
     2041 ctgtattatt ttatgcttat caaatcatct ctggaaaaga agaaaagtat ccgtctgtaa
     2101 agctagcatt gagtatgagt ttgctagtgt acagccatgt actatcaaca ttaatgagta
     2161 cagcattaat tatcattttt atttttattc gtttggtttc taaaggtttc aagaatgcag
     2221 attttattgc tatatttaaa aaattattta gtgcggcagg aatgacattg gttctgacga
     2281 gtgctttttg gtatccgatg tttgaacaaa tgctctacca aaaaatcaac aaacctagcg
     2341 tgacaaattt atatgctcat gcatctaatg tgttcgatag tctgactgaa gctatgaaca
     2401 acgacttaac tacttactca atgggactag ttggtctgtt atctttatgt attcccctga
     2461 ttttatttaa aaaattaaca aacatagaaa aaaagattta ttatggtacg tgtttgactt
     2521 ggctagctac aactagtttg gtgccatggt atcttctgca aaatacgcca gccaaattgt
     2581 tacaatttcc ttggcggatc ttaagcttgc aaataatatt ctcttctctg atattaacga
     2641 tgatattttt caaaaataga agatacaata agacaagaga actattttat ttatttatga
     2701 ctgttttgtt attaatcact ttaactgttt ctagtaaaaa aaatttcaat caaaaaataa
     2761 tttctcagcc gggacatatt gttgtaaaca aagaaacagt agaggcgtat accactaaag
     2821 gaatggatta ttttttatat gattattctc ctatcgcttc attggagaat aaggagcata
     2881 taataaaaca cgaagcaaag attggcagtc aatgggtaaa ccttccttat aaaacaactg
     2941 gtagttctat catatataga gtaactaatg agaagagcca agtaatcaca cttcctgtat
     3001 atgcttattt aggaacgaat gtagaaataa atcatcaacc tgtaaaggag caggtgaatg
     3061 ctaatggatt ggtacaaata agcggagtca aaggagttgc tgatattaaa gtaagcacaa
     3121 gttatacagt aacgactatt atttcttatc ttgttacatt aactgc
//
LOCUS       DACEPW010000232         3100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEPW010000232 DACEPW010000000
VERSION     DACEPW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.238"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            55..1296
                     /locus_tag="IW819_002564"
     CDS             55..1296
                     /locus_tag="IW819_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1245647.1"
                     /translation="MSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPS
                     DLYIVAPRMAYYMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYD
                     MAKLIHKDVFEQTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKI
                     SDITDFWGIGKRTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSF
                     LGQEYTPKSKSIGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVG
                     YSLGYLDPNGKTGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVY
                     ATGLQLNLFEDPEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGG
                     HAGGMSGLEGGKDETDSKKIH"
     gene            1268..1654
                     /locus_tag="IW819_002565"
     CDS             1268..1654
                     /locus_tag="IW819_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245648.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDHF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1850..2518)
                     /locus_tag="IW819_002566"
     CDS             complement(1850..2518)
                     /locus_tag="IW819_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1245649.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTESWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(2707..>3100)
                     /locus_tag="IW819_002567"
     CDS             complement(2707..>3100)
                     /locus_tag="IW819_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1245650.1"
                     /translation="SYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMK
                     AYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYI
                     YYYNNERIKQKLNWQSLVQFRKTTVTVV"
BASE COUNT          977 a          610 c          609 g          904 t
ORIGIN      
        1 tccgtagagt gcgttgaaaa ggatcttgat ccccttacaa ctaaattagt agtcatgtcc
       61 tatccgtccg attcgacaga aacacggggt agcggattaa tcttggcttc cagtcctgca
      121 gcaaaaaaag cttacggcat caccaatatc agccgtgcca gagatcttcc cttcccctat
      181 ccaagtgatt tatatattgt tgccccaagg atggcctatt atatggaaaa gaacagagaa
      241 atcaattcta tctataaaaa atatgcagac gaagcgaacc atcatgtcta ttccgtcgat
      301 gaaagcttcc ttgatgtaac aaatagcatg gcactctttg gtaaaacaaa tgcgtacgac
      361 atggctaaac tgatccataa agatgtgttt gagcagaccg gaatctacac gactatagga
      421 attggcgata acccgctact tgctaaacta gccttagaca atggctcaaa agacggaaaa
      481 caaatgtttg ctgaatggcg ttatgaagat gtaccagaaa agttatggaa aatctcagat
      541 atcactgact tttggggaat cggcaaacga acagcaaaac gtctgaaaca aatgggtata
      601 cattctgttt atgatttagc tcacgcggat tattatcact tgaaaagcaa gctaggggtc
      661 atcggtactc agctttatgc ccatgcttgg gggatcgatc gaagtttttt gggacaagaa
      721 tatacaccaa aatcaaaaag tatcggaaat agtcaaatcc tagatagaga ttatacaaga
      781 cgagaagaaa tcgagatcgt tatcaaagaa atggccgatc aagtcggcac tcgcttgaga
      841 cgatctgcag caaaaacaca agtagtcagc ttatgggtag gatactcttt aggctatctt
      901 gatccaaatg gcaagaccgg gtttcaccag caaatgaaaa tcgaagcgac aaatgctagc
      961 agagatctag cagatgcttt attgaccatt ttccaccagc attaccacta ccaggatatt
     1021 cggcgtgtag gcgtgaactg ttccgacctc gtatacgcga caggtttaca gcttaacctt
     1081 tttgaagatc ctgaaaagca gataaatgaa gcgaaagttg attttgttgt cgatacgatt
     1141 agaaaaaaat acggctttca atcaatcatc catgcgcata gtctcttgca aggaggacgc
     1201 gccatcgcac gtagttctct cgttggcggg catgcaggtg gtatgagtgg attagaaggt
     1261 ggaaaagatg agacggacag caaaaaaatt cattgactat aacgagtatc gagatcggcc
     1321 atttggatta aaatggggga ctgcttttgc gatggaagaa ctagtcaacg gtatcaaaaa
     1381 aaatgaagaa gaagcacgca aacagcctct tcctaaacaa caaatgacat tgcaagaaat
     1441 cgatagtata ctgacagaag cctttttatc tcgtaaagca gtaatcattc agctcaattt
     1501 aaaagatgaa tttggaagac tcttggatga ggtcgaaggg cacttcattg gtgaagccta
     1561 tgaagatcac tttatgattg accatcagcc gatattgtgg gaagacgtac ggtatattga
     1621 gttaaaggaa acaaaaaaat ggtttgtgac atgagaggtt gtgacagaag tgatcaactt
     1681 caaaaaataa gaaaggattt ttcgaaaatt gcttcttaaa tttttgtgga attttggctt
     1741 atttttcgaa gaagcaactt ctggaacaac gtatattaga tatcagaggt tgtgaaaaag
     1801 tcgcttagtt ttgagtaaca atccaaggcg ctgatttcac aacctctgtc tatccatcta
     1861 ttcggtaaga ttcatgcatg acgtcttctt tatttagtat gtagagccct ttttcttctg
     1921 accattcaac ataaatcagc tgttttacat ctgcatagcc ttctttcttc aagttctcca
     1981 taagccatga ttcagtcaat tgattggact gaagcacttt atgctgaatt tgcccttcat
     2041 ctacaagcag gatcgataaa gctttgtctt catctgattt tttcaataca ctcatttgac
     2101 cggcattctc taggataaca tgtttggcat tttcaagaga aaagcattcc tgctgtctta
     2161 agattgctcg caactgctcc atctcgatat ggttatttgt caactctgtc atgttgatga
     2221 cgccgtcttt gattagaaca gatggctcgc ctttgaccca acgattgacc ttttcatttt
     2281 tttggacgat acgttcgata ccataaatca ttactgccca aagcgcaatg gcaaatagga
     2341 catgccctac atggacattg tcatcatagg ttgattcttc taagatccca cctaaaacaa
     2401 gcgtatagac taaatcaaac ggtgtgattt ctgacatgga tttcttccca agcaaacgta
     2461 ccactaataa taaaccgatc aacccgaata ccagtttcat tgtaattgaa aaaatcattt
     2521 tttctccccc taactatctg ttaattaaat tatatatgct ttctcacgaa cacgcatgga
     2581 atatacaagg aagaaatgta tccttttact aaaaaaagga ctgtgacact gtagtgaccc
     2641 ccaaaagtta gacttttttg gagtggagaa atccactcct ttttttcatg ctctaaaatg
     2701 atatttttac acaaccgtta cggttgtttt acgaaattgg acgagacttt gccagtttaa
     2761 tttctgtttt atccgttcat tattgtagta atagatatag ctatcgatgg ctgattttaa
     2821 ttcctcgaaa cagttataaa ctttcccgtg aaaaatctct tgttttaata agccaaaaaa
     2881 attctccata agtgaattat ctaagcagtt ccctttccga gacatgcttt gaaagatttt
     2941 gtactccttc aatttgtttc tataagcttt catttgatac gcccaacctt gatctgagtg
     3001 aaacgtacga cgatagggac aatcgttagt ggtctggata gcttcctcca aaccttccat
     3061 taccgctaat gcattgggct tttcagagat acgataggat
//
LOCUS       DACEPW010000233         3093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEPW010000233 DACEPW010000000
VERSION     DACEPW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.239"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(144..1229)
                     /locus_tag="IW819_002568"
     CDS             complement(144..1229)
                     /locus_tag="IW819_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1245651.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(1549..1956)
                     /locus_tag="IW819_002569"
     CDS             complement(1549..1956)
                     /locus_tag="IW819_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245652.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(1975..2793)
                     /locus_tag="IW819_002570"
     CDS             complement(1975..2793)
                     /locus_tag="IW819_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1245653.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          918 a          483 c          423 g         1269 t
ORIGIN      
        1 acgtttgtga ttgttttcat aaacttttaa tctaaaaact ccggaataat agctatttat
       61 ttgtaacatt tgtaataaaa ttaaaatcat aaaaaagttc taatttaaaa ttgatcattt
      121 ttatagtgat cgctttttat caactactct agccaatttt ccgtactaac tgttggtaat
      181 tcgttaaatg tttctctttg ttcgtttgca agctttgccg caatagcttt ttcaataaac
      241 cagtttttta catacacaaa aatcaaattc tgcaaattat ttactttctc agtcttttgt
      301 ttttgataag ctttcagcaa cgtcatatat aatcctttat ccgcatcaat tccgtaagat
      361 tcaagttctt catacacaac taccgtcttc tcaagttcct cggctttatg ttttgctttg
      421 tgtattgtgg tgacggtttg ttgggcttct tcgtaagtgt tagaaaatgt ttttatcaag
      481 cttaatacct tttctggtac aaatgtgttt atgctgtctt ctttcatgat ttcaacgaag
      541 ttatctagca aaacttgatt ttgtaattgg tctgtttcag tgtcttttaa gtgtctttta
      601 gtgtctaaag atttattaat ataataatct tgtgcgattt ccgcatttcc agtgttatca
      661 agggtttcag ctgttttttc tcgacgtgcg atttccgcat ttccagtgtt atcaagggtt
      721 ttagcagttt cctctcgacg tgcgatttcc gcattccgag aagaggttga tttatcaata
      781 gtttcgctta aatcgtcatc gaaagaattt tgaataaaaa gatcatcggc acttatttca
      841 ggttgaagta aatagtattt atttttttca ttttttttcg tttttgggtt aaattctcct
      901 tttacgactg aaatcaaatt atatgtttct aactcgttta aaatggtttt tactttgcct
      961 tggtagcaat ttaatttgtc cattaggtct gcttgtgtaa attccaaata taatagacca
     1021 tcatcgtcga tccatttttc atgtaaggct ctacgaaatt catttttaaa aagcatatag
     1081 gctatttttg agctgtcttt cattttagag tatttagaat tctgaattaa aaaactagga
     1141 aatttataat atttttcaat ataaacattc gttgctttat atccccaatt tttaattatc
     1201 attttttgag attgattttc tgtagacatt aaatttctcc ttcttcttag aaacttgtat
     1261 atattcagga ttttcaacaa gtgaactata ccatgtatta agggtgaata aaacactttc
     1321 agtatttaag gtataagtta tatattaaaa aaataagtat aagttatact ttaaatacaa
     1381 ataatgtata acttatactt taaataataa ttttataatg ttgatttaat cgcaaattta
     1441 cacgttgttt attaagttat accttaaata tttaatgtat aacttatact ttaatattca
     1501 tttatctagt ataacttata ctttaattga ataccgcatg aaaaaaaatt attttggtaa
     1561 tttttcaaac tcttcttgta tttcttctct tatgtgttct tcttttgcta ctcctagtct
     1621 atgctggata tatgagtctg ctaatgtatt aacaattttt tcgaaagaaa ttctcccttt
     1681 ttcactttgt aactccgtca tatagtcttg taaaattgag agtttgctat ttaatgccgg
     1741 agagatcttt ttaggaactg ataccttgtt tcttttatca ggaattgaaa ctggtcggcc
     1801 tctttttttc aatgtaatat ttcttgtgtt actcaatgca ctattgctaa agttatattc
     1861 accgcttatt ttcacatctt ggcgtttgtt attgggtaat aatggctcaa attcaccatt
     1921 ctccatcatt ttaggaatat caaaatcttt aggcattact tatgcaactc cttattattt
     1981 atttaaaaac tcttgtctag catctagttc tgccaaaata tttataaata cttggtgtgc
     2041 tttataatcc cattgatcaa aatttccatt tttgtatttt cttaaatgta taccaatttt
     2101 agaatacctt ctcaatcttt tttgataatg aatgagattt ttggatacca cggttccgta
     2161 tttttcaatt gcttcttcta ggatttcttc atcaaaatca tcatttggtt ctaccataca
     2221 aggtacaata tctataattt gcaaatcaag atcgtatcta tcgaccatga atttttgata
     2281 atttacatat ttgttgactc cctctaagga ttcttcagct gtttgaaatg aaattatcga
     2341 atagtctgaa gctgccatag cattatcgga aaattcgcta atggttggtg gtacatcaat
     2401 aaatatttct tgataattgt tctttattgg aagaagtaat tttttcaaga cagtaacttg
     2461 gtctaattca ttttcatgtt tattagcaaa agcggagaat gatcgaaaac tagtgtcaca
     2521 tgccagaagg tcaagatttt catttacctt aataatacta cttgctaatt ctccatttgc
     2581 gacaccattt gttatagtgg ttttgatatc ggttataccg aaagttcttg ctatgacttg
     2641 tgttaagttc gcttgagggt ctaaatcaat aataagcgtt tttctatttc ttatcattgc
     2701 attgtcataa gcaagcatgc tgactatctt agttttacct actcctcctt taaaattgcc
     2761 aactgtaaat gtagtccctt gaaaagtatc catgtaaatc ctcccaatct ttaattgata
     2821 attaaagtat aacttatata tcggaaatgg tcaacattaa aagtataact taaataataa
     2881 tatctaaaaa cgtttggata tagcggtttt taataataaa agtataactt ataccttaaa
     2941 tattgctttt cgaaaatagc tattctagtc tatctgtttc tttacttttc gctgctgatt
     3001 tgaattgaat tgatcatatt cattttctag tattaataac ttttcgtata catcggtcat
     3061 attcatacta gaagcaagac ttagatcata tac
//
LOCUS       DACEPW010000234         3093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEPW010000234 DACEPW010000000
VERSION     DACEPW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.240"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..1115)
                     /locus_tag="IW819_002571"
     CDS             complement(75..1115)
                     /locus_tag="IW819_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1245654.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1464..1790)
                     /locus_tag="IW819_002572"
     CDS             complement(1464..1790)
                     /locus_tag="IW819_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245655.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1777..2565)
                     /locus_tag="IW819_002573"
     CDS             complement(1777..2565)
                     /locus_tag="IW819_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1245656.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          920 a          500 c          424 g         1249 t
ORIGIN      
        1 ttgtaataaa attaaaatca taaaaaagtt ctaatttaaa attgatcatt tttatagtga
       61 tcgcttttta tcaactactc tagccaattt tccgtactaa ctgttggtaa ttcgttaaat
      121 gtttctcttt gttcgtttgc aagctttgcc gcaatagctt tttcgacaaa ccaatttttt
      181 acataaacga aaattagatt ctgcaaattg tttacttttt cagttttttg tttttgataa
      241 gctttcagca aagtcatata taatcctttg tcagcatcaa ttccataaga ttccagttct
      301 tcataaacaa caactgtttg ctctaattct tcagcttttt tctttgcatt atgaatagtt
      361 tggacggttt tttgagcgtc ttcgtaagta ctagaaaatg tttttatcaa gtttaataca
      421 ttttctggta caaatgtatt tatactgtcc tctttcatga tgtcgacgaa gttatctagt
      481 aaaacttgat tttgtaactg atcagtttca gtgtctatta tgtgtctatt agtgtctata
      541 gaattagaat agaatagatt gtctgcgatt ttcgcaatta cggttgagtc cttgatatga
      601 ctggattttg ccgtatttgc gattttcgca attacggtgg tatcattgat ttctcgcggt
      661 tttgccgtat ttgcgatttt cgcaattacg gtggtatcag tattgtcttc ctcttccatg
      721 attttaaaga cgtcttgatt cgttacttct ggcttgccta agtacaataa attaggcatg
      781 gattcaatct taccgtcacg tttatttact cttcctctct tctgtttcag aagacctgct
      841 ttttctaatt cctttttgat tttagatact ttaccttcgc ggcattgtaa caatttcatt
      901 aattcttcaa ctgtgaatat gaaaaatata ttatcatcag aatctaccca gttatttttg
      961 actgaataat caaatcgatc tttaagtagc atataagcaa tttttgcatc atttgataga
     1021 tccatgtatt tttcgttagt aaaaaatacc ttaggcattt gataatattt ttcacgataa
     1081 atttcttttg atttgtaata attaaaatca ttcatagcca atcctctttc tgtaggatag
     1141 cgttttgttc aaaactatgt tacaatgaat taaacaaata tatctatcca ctatatattt
     1201 ttctactgct gataagtcgc caaactttgt taaagcagta gtcttttttt atcttttttt
     1261 agtttacctt aaaaaataaa ttaaagcaaa ccgctctatt tcaaggttta agctcatagt
     1321 aactatatca cgatattaca ataatattat atcacgatat cgtaatatta caatatgaag
     1381 ataagcgact tatcagcttt gatactactg atttttataa aaaaaatatc acgatattat
     1441 gatatcgtga tattgaatgt aaattaaatt ttattttgtt tctttgctag tttatatcct
     1501 tcaatcaagt attcttcagg tatggacttt aatagcgcat cttctataac gtctggttta
     1561 cttttgtctg attcagcaat agtcgtccaa tatttgagtg ctgacattag aattggattt
     1621 atacgaatgc tatctgctgt tcttacacta cttccttgat cttttatgac tttacttata
     1681 tttttcttgg ttaattcgat ttcttctact tcttttgatt taaaattctt tttattttta
     1741 tttcctagta atctacttac tgcagcttga ctgcgattat cttctgccat caaaatcacc
     1801 aacttctttt aaatattctt ctgtaaatgc tttatagtca tcttcagctg ttgagggttt
     1861 attaaaattg attagatctg atccagcagc ttgcgcttcg tcaacggtta ttgtcctacg
     1921 aatataagtt ttgaaaattt ttgcaccaat gactctagaa atttcggttg ctgcagacgt
     1981 catttcttta gaaatgttat aacgcttatt atacttagta aatagaactc ctcgtacttc
     2041 aagcttgtcg ttcgtatatt cttttaccgt ttcaatagtt tcacttaact ttattagtcc
     2101 ttttaatgag aaatacgcag cttcaactgg aatgataatt tcatcagaaa cagtaaatgc
     2161 gttgactgta agcattccta aacttggtgg acaatcaatt ataatataat catagttgtc
     2221 cttgattggt tgaagtactc ttttaagcct gtgttcactt cctacttgag taaattccct
     2281 ctctgcagaa gaaagggcta tgtcggctgg caaaacatca attcctttat agtccagaat
     2341 agcatctgta atgtcagcct ccccttttaa tacttcataa attgtaggtt ggtttgttcc
     2401 atctatatcg aaacctacat tatctgtagc atttccctga ggatcaatgt ccactaaaag
     2461 tacattttta ccgtaaacaa atttcaaaca tgctgctaaa ttaacgctag tcgtagtttt
     2521 agaaactcca cctttttgat tggctactga caatattctt gccattaaaa tatcccccat
     2581 tcatcttata ttataatata attatatcat aatattgtga tattgcaata tcgtatatgc
     2641 attatattgt aatattgtgt gattattttg ttttaaagat atatatcggt tcggatataa
     2701 agattattca tatgttattc gaaattaaaa taatcacttt ttattacttt aaaatttttt
     2761 attaatgata agaaatatgt tttgcgagca gcaactagaa taatttaaac attactaaac
     2821 tgtctatatt gttactaata attcccttct tatagtgcat tttcttagaa aaaaacaata
     2881 ttacgatatc atgatattaa gatatcgtaa tattatacaa ttgtgattga tattatatat
     2941 gtttttttat ttttttatca tgtttaatct tgaaggaatc atattctttt tctaaagaca
     3001 ggagcttttc ataaacatca gacatgttta aactagaggc caggcttaaa tcataaacga
     3061 atgaagcagg taatgatttt attctacgtt gtc
//
LOCUS       DACEPW010000235         3076 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEPW010000235 DACEPW010000000
VERSION     DACEPW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.241"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            110..1117
                     /locus_tag="IW819_002574"
     CDS             110..1117
                     /locus_tag="IW819_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1245657.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1129..2133
                     /gene="neuB"
                     /locus_tag="IW819_002575"
     CDS             1129..2133
                     /gene="neuB"
                     /locus_tag="IW819_002575"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ1245658.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2130..>3076
                     /gene="neuC"
                     /locus_tag="IW819_002576"
     CDS             2130..>3076
                     /gene="neuC"
                     /locus_tag="IW819_002576"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ1245659.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSK"
BASE COUNT         1147 a          393 c          590 g          946 t
ORIGIN      
        1 gttgattcgt tacgttttga catcattatt gataattagt ttagttatat attttttaaa
       61 gattaagaat cgaattatag atgactataa ttcataggag gagatataag tgtttaaagg
      121 aaaaacaata ttagtaactg gcggaactgg ttccattgga agtgaaattg ttcgtcagct
      181 tttaaaatat gaaccaaaag ctatcagaat ttatagtaga ggtgaagaaa aacaatttta
      241 tatgcaacaa gagctctcaa atcacactaa tttgagattt ttaattggtg atatcagaga
      301 ctctcaaagg attgattatg catgtcgtga tgtggatatt atttttcatg ctgccgcaat
      361 gaaacacgtt ccagcaagtg aatataatcc tatggaagct gtaaaaacaa atgtgatggg
      421 aactcaaaat gtaatagatg cagcgatcaa aaataatgta aaacattttg taggtatcag
      481 tacagataaa gtagtgagtc ctactaacac catgggtgcg acaaagttat tgagtgaaaa
      541 attaataata gctgccaatc agtataaagg aaaaacagaa acaatatttt cttgtgtaag
      601 gtttggaaat gtcatggggt caagtggatc tgttattcct ctttttagta aacaaataaa
      661 aaacaataaa gatattactt tgacagatgt tgatatgaca cgatttatga tgtcaatacc
      721 ggatgctgca agattagttc tgtctgctgc tatgaattca aaatttggag aaacatatgt
      781 cttaaaaatg ccggcaatca atattttaac tttagctcaa tcattaataa aaatatataa
      841 tgatacacat gatgaaaaat atagaggtga aataaaagtt gtaggtatca ggccaggaga
      901 aaaattatat gaagagctaa tgactacaga cgaaattgaa cgctcatatg aaaatgataa
      961 aatgtatatc attccaccac actatcatgc tactgacgaa ctgtacaaag attatgaaaa
     1021 aattaaaatc actgcatact cttcagatgc aacagaatta atgagtggac aggaatttat
     1081 tagaatcgca aaagagaata atgtcgtgac ttggtaagga gttttatgat gaaatcagaa
     1141 gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa gtctagattt agctttagaa
     1201 agtattgata aagctaaaga atgtggagct aacgcggtta aatttcaaac atttaaaacg
     1261 aataagctag tttccaaaaa ggctccaatg gcaaaatatc agaaggataa tcttaaagag
     1321 gacagttctc aatttgaaat gctaaaaaaa ttagaattat ctcaatctga ttttattaga
     1381 ataaaagatt attgtataca aaataaaatt gattttttgt ctactccttt tgatgaagaa
     1441 agtgcagact ttctaaaaca aattggtgta caaggattta aaattggctc aggagattta
     1501 acaaatttac cttttcttag aaaaatagat atgtatgggt tgccagtttt attatctaca
     1561 ggtatggctg atattcatga ggtcagtgag gctgtagatt gtttcataga tagtccagtt
     1621 actattttac attgtacttc aaattatccg gctagttctg aagatattaa tttattggcg
     1681 attaacacat taaaagaaat gttcgggata cccgttgggt actccgatca ttcattggga
     1741 tatgatgttg gtatttgtgc tgttgcgatg ggtgctaagg taattgagaa acattttacc
     1801 ttggataagg aactacctgg tccagatcat aaagcttctt tagatcctca ggaatttgca
     1861 gattttgtaa agcatattag aaacactgaa atatttttag gtgatggtgt taagcgagca
     1921 atgccttctg aacttagtac aaaagaagta tcaagaaaaa gcctagtggt cactgagaat
     1981 ttaaatgtgg gagatattct tacagagaaa tctcttacaa taaaacgtcc tggaaatgga
     2041 atagctccta agtttttata tcaatatatt ggcaaaaaag ttaaaagacc aataagtgca
     2101 gatagtgttc tgcttgagga agatgttgaa tgaaaaaaat acttatagta agtggaacac
     2161 gagctgatta tggaatatat aaacctgttg ctgagaattt actacaagat aattgggatg
     2221 tatcatttgt tgtttctgga atgcatttat cccataaata tggatttact aaagaattga
     2281 tcttagctga cggatttaga attagtggag aagttcaaag tttgttttta gagaacaata
     2341 agggaaacat ggctcggtct gtggcattag agataaatgg tttcacaaat atatttgaac
     2401 aaactagccc tgattttgta ttattactag gggatagagg agaaatgttg gcagctgcga
     2461 tagcttgttt gtatcttgga atcaaaacag cacatattca cggaggcgaa gtgtctggta
     2521 ctgtagatga atcaataaga catgccataa cgaaaatagc atctgttcat tttgctgcta
     2581 cggaaaaaag caaagagcgt ttaataaaaa tgggggaaga ttcttggaga gtttttcccg
     2641 tcggagctcc tagaatagat actattttaa ataatagatt acctagtttt agagatataa
     2701 aagaaaaata tcatctaaaa tttgaaaaga aaaaatacgt tttgcaagta tttcatccag
     2761 ttgtaaccga actagaaaat atagaaaaac aaattttagc tttgatcaat tcaatggaac
     2821 aacaatctca accaattatt tgcattttac ctaatagtga tgctggtagc gaaattatca
     2881 gaaaaatgta ccagaaatat ttaaaaacgg atattattta tattacgaat ttagagccgg
     2941 aggaatacct gactatatta aaagagtgca aatttatgat cggaaattct tcttctggga
     3001 taattgaagc tgcatcattc aaaataccag taataaattt aggaaccaga caaaatggac
     3061 gagaacaatc taaaaa
//
LOCUS       DACEPW010000236         3050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEPW010000236 DACEPW010000000
VERSION     DACEPW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.242"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(174..1589)
                     /locus_tag="IW819_002577"
     CDS             complement(174..1589)
                     /locus_tag="IW819_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1245660.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(1606..2922)
                     /locus_tag="IW819_002578"
     CDS             complement(1606..2922)
                     /locus_tag="IW819_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1245661.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
BASE COUNT          938 a          673 c          481 g          958 t
ORIGIN      
        1 gttgttttta ctttgctatt cttattactt gatgcaggac agctttttca caacctctca
       61 ataaacggtg ttcaatcagc tgaccctggg tattagctca aaacgctgga ttcacaacct
      121 cttttatttc aaataaccag taaatacaat gaacatctac aaaaaacttc tatttattta
      181 tagatttcgt caggcaaatc caattctctc ttatcaatca aagtctgata ccacgaagca
      241 gattttttca atgttctgct tcgttcatcg ttcaattcga ttcgaactaa tccatatcga
      301 ttcttaaatg cattcattgg tgacacacag tcagtgaatg cccataacat atagccttcg
      361 caatttgcgc cttcttctgt tgctttgatc aagtaatgca aatgctgtcc gataaattcg
      421 atacggtaat catcttgcac gattcctttc tcatccatat actgttcttc atattctctt
      481 cccataccgt tttctgttat cagccaaggg atattatgat aattttcttt tagatacatg
      541 gctatatcat acatgatttt cggatagatt tcccatcccc tggaattatt cattttcttc
      601 ccaggtaaat caaagacttc ataatatcgt tcaggatgga aaggtgtctc ttcattccat
      661 tgataacgag gggcgcgaac gcgtttagga taatattgat tgattccaag gaaatctact
      721 gtatgatgct gaatcgtttc taaatcctct gttgcaggat cgaaataaat cgattgtttt
      781 tgacacaact ccaggaactc ttctgtgtag tatcctttta ccatcggatc aaaaaatact
      841 cgattataaa acaaatcata catataagca gctttttggt cttctggaga attggatcga
      901 gcatagacca tttcaggatt taaaatacag ccaatccttc caccataatt tccgctgcgg
      961 taaatctcta ctgctttagc cgttgctaaa actttatgat aattccacat catccacttt
     1021 tttgtgtttt gttcatgcgg ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg
     1081 attggttcgt tgaatgtaaa ccagtttttt accctatcac cgtaacgatc aaaggcaatt
     1141 tgagcatact tagcataaaa atcaactact tttcttgaag accagccatc atatttttcc
     1201 aaaagaaaag ctgggacttc ataatgttcc aaacaaatca tcggttctac gtctgcttcg
     1261 atcaaagagt cgatcatttc acttatatgc tgagcatatt cttcatctac tatacagttc
     1321 tcataatcag taaaaaaacg agcccaattg atagaagtcc gataatgtgt cagatggatg
     1381 gatttcatca gatccacatc ctgctggtat tgttccataa aattagttgc tacggctggg
     1441 ccatacccat tatgccaaac aaaatgttca tttttatacc agctatctat aaacgaatcc
     1501 tgtccttctt tttttccttt ccaaccttcc gtttgccaag cagaagatgc agcaccaaga
     1561 ataaaatttt ttggaatagt aattttcatg atttcctcct gttttttaga tcccatcatt
     1621 ttccacacga tttgctactt ttacaaatgg cagatagatc aaaatagaaa caataataca
     1681 aacaagttgc gtaataactg caccgatatt tcctgctgtt gccagataag cattgacgat
     1741 cggcggcatt ggccaaggga ccattacaac tgctttcgcc gcaaatccta aagcggttgc
     1801 aaaataacca atcgttccag tgataagagg cgttaagata aacggaatag ctaaaattgg
     1861 attcagcatg atcggcatac caaaaataac tggttcattg atgttgaaga tcccaggagc
     1921 aagggataac ttggtgattt gtttcatatc ttcccgtttt ccaactaaca atactgcaat
     1981 cagtaaaccg atcgtgactc cagacccgcc catactcata tacatatccc aaaatggcat
     2041 agtaataatg ttcggtactt cttttccagc ttcaaaagct tccgtattta cagcaattgc
     2101 cgctaaaagc aacggttccc gtatcggctt caccatttga tttccatgga taccaatcac
     2161 ccaaaagact tgcgcaataa acatcaatag caaaataccc ggtaaccctt gtacgacacc
     2221 ttctaatggg cgttgtacga cattgtaaat gatatcataa aggtacattc ctgtaagttc
     2281 tttgatgata aatcccgcag ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc
     2341 agaaaaacta gcagaaacat tcgttggcac ggtatcaggc attctcagct gtagtttctt
     2401 ctgtttcctc aaccagcaat aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa
     2461 taaccctttc gtatctgtat attgtttagc caatacgttt tctactataa cagtctgatt
     2521 ttcattcact aattcataaa cagttggatt aacagataaa taagacatca ctgcgatgat
     2581 accagggaat acaccatcca ctttattcag cttggctaat tcgatcccta tcagaaaaac
     2641 agcatagatg gtcaagaaac ttagtgtgac atagctgatg gcagatgcga taggctttaa
     2701 ttcttctaaa aaacgcaaga aacttatctg tgctaagcca ttttccgagt caaagaccat
     2761 tgctgaaaac aatgtgccaa aagcacccgt aataattacc ggaatcaatg cagcaaatgc
     2821 gttttttatc accatgatat accttaaatt attgattctc gttgccacgt tccctaaaac
     2881 attggctaat ttatccataa aactcattcc agatcccctc attcctgctt cttctccttg
     2941 acgtcatctc tttttgtcta ttagtcagcg cttacatttc gatgaacaga aactatttac
     3001 tcctcttttt taccaaatat aaaacaaaaa ttcatctgat gtatttatca
//
LOCUS       DACEPW010000237         3048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEPW010000237 DACEPW010000000
VERSION     DACEPW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.243"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..637
                     /locus_tag="IW819_002579"
     CDS             <1..637
                     /locus_tag="IW819_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1245662.1"
                     /translation="CVQEKSFRLIQLHVESKGEFLKKEWQDTILELFRYSADFFFYTD
                     TDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFHSPGTDLVPLFAEERKI
                     FLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIELIKQNEMEVIIQELWNE
                     LGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFCYLLLLHEHN"
     gene            complement(718..1053)
                     /locus_tag="IW819_002580"
     CDS             complement(718..1053)
                     /locus_tag="IW819_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245663.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(1120..2490)
                     /locus_tag="IW819_002581"
     CDS             complement(1120..2490)
                     /locus_tag="IW819_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1245664.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(2568..>3048)
                     /locus_tag="IW819_002582"
     CDS             complement(2568..>3048)
                     /locus_tag="IW819_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1245665.1"
                     /translation="YVEEYGMKSITARELAEFCGCSTYPIYTHFKSISGLKEKILEEI
                     TVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKSDLDMDSLFKRPFFSYVKG
                     HTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDKNEVTDEYLENILKEQSP"
BASE COUNT          879 a          635 c          489 g         1045 t
ORIGIN      
        1 ttgtgttcaa gaaaaaagct ttcgtttgat ccagcttcac gtcgaatcaa aaggagaatt
       61 cttaaaaaaa gaatggcaag atactatttt ggaattattt cgctattctg ctgatttctt
      121 tttttataca gacacagatg cgctcttagt cgaacaaata aaccgtgatt atcttgatgc
      181 tactgaaatc aacggtattt ttctatcttt agatgctgat tttgatacga caacgtctgt
      241 attcgttggt tctttccatt cacctggcac cgacctcgtc cctttgtttg ctgaagaaag
      301 aaaaattttt ttaacagagc aaaacaatct ttatactcac agccgtactt tttctatgca
      361 agatgtggct ttacattact atacaaaaga tactgtaaaa gatagtaagt tgatcaaaag
      421 ttacatagaa ctgatcaaac aaaatgaaat ggaagtgatc attcaagaat tatggaatga
      481 attaggcaat gtcagctctg ctgcaaaaac actgtttctt catcgtaata ctctaaaata
      541 tcgaatcgaa aaatttcaag aacagtctgg tttcaatcta aaaaaagcca acgatttact
      601 gttttgctat cttttattgc ttcatgaaca taattaaaga tagattaaag gggttgtgac
      661 aataattttg tcacaacccc ttatttcgaa tgaatagtgt tcaaaagcta gtttctctta
      721 catcattttt tcaacaaaat gcattagccg gtcttttaca tcttcggttg aatctttgat
      781 tgaatcacca taaacagaga ttttcttttt tcctgatttg atttttgcta acattcccat
      841 caaagaatcc aagtcactat ctgaaagacc atctacgatt tctagtaatt tttcatttcc
      901 atcaaatttg tcatagacga atttttttac tttcgtccga gtagtcatat gatggatctt
      961 ttccattact tttcccgata caacgactgc tgtcgctaca ctggcagcgg ctgctacgct
     1021 taatccgatt gttacttttg ttgatgtttt cataagtatc agctcctttt tatctatctc
     1081 catcatagca tagacaaaaa aataaacgaa ctaatccact cagtgaaatc tcaatccttt
     1141 agggaaaaag ttctttactg ttccttttac ttgtttccca aatccgacag gtatcttatc
     1201 taatactagc aaaacccatc cttgattccc atcacgttga aatgtttctc ctgctgtgta
     1261 agattgccat tccttttgag tgatcggcaa gcaaggtata ttctctatct tttttgtagc
     1321 aagcgctaat gcatagctag gttcaaatcg attctttttg aaatctccta aatgcaaacc
     1381 tgtacgtacg acttttaaac catcgagaga aggggctaac tcaggaactt cccaaagatg
     1441 gtcgttgaac actaaaagtc ttccagtagc ttcataatga aaatcatttg aaaactccgt
     1501 ccagagtttt tcctgctctt ttgtcatttg cactttactt ttttttcgtg tcttcttttc
     1561 tttatgcatc tgattttgtc catgaaaagt cagctttgca acaaaatgtc cttcaccctg
     1621 atctttatga ggccatatac gtattgtttt ttccaaacca gctacgcttc cccattcaga
     1681 acgtcctgaa ctaacggatt gagtcaaagg aatctcctca atcgttactg gataattttc
     1741 tacaagccag ctaatgatct cttcattttc ttctggtgca aacgtacaag tcgaataaat
     1801 cagttgtcct ttatttttta gcatttttat tgctgaagac aaaatctctt gctgacgttt
     1861 ttgacagtat agcggagatt cttctgtcca ctcttttatt gcatttggat cttttcggaa
     1921 cattccttct cctgaacaag gagcatcaac cacgatccga tcaaaaaagc ccgaaaaatg
     1981 agggacaagt tctgcgggtg catgatttgt cactattgcg ttcgaaactc cccatcgttc
     2041 tatattttct gataaaatct tcgcacgttt cggaaagatc tcattcgtca ctaataaccc
     2101 tttccccttc atttgagcag ctaattgggt tgatttacca ccgggagccg cacacaaatc
     2161 caaaactttt tcaccgggtt ttgctgcagc agctgttcca acgatcattg cacttggttc
     2221 ttggctatat tcatacccag cttgatggag gaaagacttt ccattgacag tgccaagaaa
     2281 tccctcattg ctgtagggag cgggttgtaa ttcttcgcta tggtaagttt ggaccatgtc
     2341 caatcctgcc ggttttagtg gattccaccg aaatcctttt ttcacgcttc cttgttcaag
     2401 cgcagaaaaa aaatcggatg cttcttcgcc taaaagaaga cgatattttt tgataaattg
     2461 ctgtggtaac gttgtcgctt cctccttcat tccaatgttt cgttagacat tttacaatct
     2521 tttttacgaa aaataaaggt ggctgatcta ctttttcatg ttgctgccta aggtgactgt
     2581 tcctttaaga tattttccaa gtattcatcc gtcacctcgt ttttatccca aacagcgttc
     2641 atcatttcaa gaaaatcttg agaagaatca tctgagcgca aacttcccaa tgcattaagc
     2701 cataccaatt caagcgactt ttcttctttg atcttcgtgt gacctttgac atatgagaaa
     2761 aatggccgct taaatagcga atccatgtct aaatcagatt ttgcaatgat ttcacgaata
     2821 ctttcatgga tagaaaaaaa atctttcaat aagtagacaa tgcttaaaac atcatcggaa
     2881 ctgcattcag ctaagtagtt ttcaaaataa acagtgattt cttctaatat cttctctttt
     2941 aacccgctga ttgatttgaa atgtgtataa atcgggtatg tagaacaacc gcagaactct
     3001 gctaactctc ttgcagtaat tgacttcatt ccatattctt ccacgtat
//
LOCUS       DACEPW010000238         3036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.244, whole genome shotgun sequence.
ACCESSION   DACEPW010000238 DACEPW010000000
VERSION     DACEPW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.244"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            199..1032
                     /locus_tag="IW819_002583"
     CDS             199..1032
                     /locus_tag="IW819_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1245666.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLAKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEEGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1134..2834)
                     /locus_tag="IW819_002584"
     CDS             complement(1134..2834)
                     /locus_tag="IW819_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245667.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNIRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFNKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKHLRA
                     "
BASE COUNT         1075 a          499 c          606 g          856 t
ORIGIN      
        1 gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa
       61 cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga
      121 acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg
      181 aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca
      241 gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg
      301 attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta
      361 atgaagttct gtccggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt
      421 ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac
      481 atcggttatg ataaatatga agaaggaaat gcactgcttg cgaaaaaaac attggaaagt
      541 caggtcctta ccgtctccca atctcaaaag aaagaagatt atcagacacg caagatcttg
      601 attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg
      661 tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg
      721 gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt
      781 tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa
      841 gaaggaaaag cagctactgt ggaaaagaaa atcgatggtt gggaacaaaa tacagaaaaa
      901 ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt
      961 tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat
     1021 gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca
     1081 aacgattata ccgtttggag agttttttga tcggatgcat ttatcccttt tgcctaagca
     1141 cgtaaatgct tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa
     1201 gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg
     1261 attccttttt gctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat
     1321 gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt
     1381 ttgtccccag tgtttttcca cttgattgtc agaagaccgc ctggatgtgt ttttataaaa
     1441 gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc
     1501 ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata
     1561 tctttgttga agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga
     1621 ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt
     1681 gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac
     1741 tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga
     1801 aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga
     1861 atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat
     1921 aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga
     1981 aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt
     2041 tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc
     2101 caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct
     2161 tttccttttt cagtctggtt aaaaaagtaa taaagataaa aaggaatata catcaaagct
     2221 aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg
     2281 ataacaggaa actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa
     2341 aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta
     2401 ctcatgaata aaacggctac tggtacactt acccaatttc gaatattgac atatctcagt
     2461 aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa
     2521 tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg
     2581 taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg
     2641 aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca
     2701 tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg
     2761 aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta
     2821 gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ttcatgaaaa
     2881 tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat
     2941 aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta
     3001 gctttttcat gacctctcgg taaacggtgt tcaatc
//
LOCUS       DACEPW010000239         3010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEPW010000239 DACEPW010000000
VERSION     DACEPW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.245"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..326)
                     /locus_tag="IW819_002585"
     CDS             complement(<1..326)
                     /locus_tag="IW819_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1245668.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENL"
     gene            complement(510..1727)
                     /locus_tag="IW819_002586"
     CDS             complement(510..1727)
                     /locus_tag="IW819_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1245669.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2110..2193
                     /locus_tag="IW819_002587"
     tRNA            2110..2193
                     /locus_tag="IW819_002587"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2144..2146,aa:Leu,seq:gag)
     gene            complement(2433..>3010)
                     /locus_tag="IW819_002588"
     CDS             complement(2433..>3010)
                     /locus_tag="IW819_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245670.1"
                     /translation="NILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTM
                     GQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIY
                     LSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKEL
                     MKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT          961 a          614 c          478 g          957 t
ORIGIN      
        1 tacagatttt cagaaatatc agaagatttt agtacttgtt ctaacttccg ataatcacgt
       61 ttttggaaac aactatccaa cagttcaaat aatttatcgt ttgtccgagt agtttttcga
      121 gagagattca tcacatgatc aatcgttacc cctagccgtt gacaaatctg catcatcacc
      181 aatacattcg gcagttcctc attattttcg atccgactta atacactttg tgaacaaata
      241 tcatgcgaaa gcattttttg cgtcattttt tgttcttttc gaatctcttt aatgatcgtt
      301 ccaaaggact cgattaacgt ttccataagt atcctcccca aaatatgcgt attcgcattc
      361 tattcgtatt ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa ctaaataata
      421 tactttagtt atttaagaaa actccactag aaagagagcc tcagtgtgtt tgtctgtggc
      481 cctcctactg aaaaatttgg tttttaaaat catttttggc gataggttgc taggaattct
      541 ctcattttct tagctgtttg tttcaaatca tccattttag gaagataaac gattcgaaaa
      601 tgatctggct ggttccaatt aaatccacca ccatgtacaa gtagaatatg atgttcatgt
      661 aaaaagtcca aaacgaattt ttcatcatcc aaaatattga atcgctttgt atcaattttt
      721 gggaatatat aaaaagcagc ttttggtttt acggctgaaa gaccaggaat atcattgatt
      781 gcattataga tatattctct ttgttcatag atccttccgc ctggcaggag cagctcatcg
      841 acactttgat aaccgcctag cgcggtttgg ataatttgct gagacaatac attggaacac
      901 aaacgcatgg aggacaacat attcagccct tctatatagc ctttgacgtg tgacttatct
      961 ccacttagga ccatccagcc tactcgaaat cctgctacac gatgagattt ggatagaccg
     1021 ttcagcgtaa caacgaacaa atcaggcgct aatgtagcaa tcggaatatg ttctagacca
     1081 tccatcacta agcgatcata gatttcgtcg gaataaataa tcaggttatt ttgtcgagct
     1141 acttcaacga tttcttctaa taattctttt ggataaagtg ccccagtagg attattagga
     1201 ttaatgataa caatagcttt tgtccgactc gttatttttg atttgatatc atcaatatca
     1261 ggataccatt ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc agctaatgca
     1321 atagaagccg tccacaacgg atagtccggc attggaacca gtacttcatc tccattgtcc
     1381 agcaacccct gcatacacat cgtgatcaat tcgctcactc cgttgccggt gtagatatca
     1441 ttgatcgtca cattcggaaa atgtttcagt tggcaatatt gctcaattgc ttttctagca
     1501 gaaaagatcc ccttggaatc tgagtagcct tctgagtcac gggcattcat gatcaaatca
     1561 cgaatgactt cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt gttcaatttt
     1621 aaaatactaa tgccctcttc ttgcatccgc tccgcttctt ctagaacggg accgcgaaca
     1681 tcgtaactga caccttctaa tttgttggat ttttcaaatt ctctcatcac ttagccaatc
     1741 cctcatttca aaaagttaga actttccaaa ttttttgtta tttactattt caatatcata
     1801 ctccatccta gaaaattttg ccacttattt aggaaaaacg taagtattaa aaaaacacaa
     1861 acaagcgttg agaacttaat tgtcatgata gtcattccat agattcgctg cccaatccat
     1921 tgcaattcca caaatgattc ctagcataaa accaagcaaa aaacgtccga atgcaagtcc
     1981 aatcaataca cctaagatca ttcccagtcc tgaaaattct ccgtcatatt tctccatctt
     2041 aataagtcct cccatttttg agcttgacaa ctatgtaaaa cctcgctatc attagtgtgt
     2101 tattattacg cagatgtgtt ggaattggca gacaagcatg attgagggtc atgtgagcgt
     2161 tagctcgtgt gggttcgagt cccatcatct gcactacaac tcgtgaagtt aattttgctt
     2221 cacgagtttt tttgtttgaa cgtttcttta tgccattata tacatatcac tgtatccttt
     2281 acaaaaagaa cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg tcggaaacaa
     2341 gctactcgta gaaacttgca agtgttaaaa caattaatcg aaaaacgagt aaaatccctc
     2401 acctaatact aaagcgaggg attttttgta tattatctgt ttatcaccgt tattataaag
     2461 gtacaaatac caagcacggt taatccaaat tgcataaatt catttttttg tacactcatg
     2521 cttttcatta gctctttaac tgccattagt actaagatgg cgataacaac ataaagtata
     2581 tagtacgtcc gcattccgga agcccatact gtgggagaaa atcccataat taaccttgaa
     2641 caaaatccca ttatcatcaa aataactaaa aaaatagaag ttttcttatt gtcaaaactt
     2701 aaataaattc caacaattaa acatagtaat aatgccaaga ctactaaagt agctatccaa
     2761 gtacctggat aaaaaagtga cagtttcgtt cctaactctg taaagagatt attcaggtta
     2821 ctactactcc aaatcattgc ccttttattt ccattgacgt aagtaaagct ttgacccatt
     2881 gtattaccaa aataaataat caggtttaag aaaaacggta atgcagtcaa tatacgcaca
     2941 taatagtttt gacatttcct atatgttaga aggaaaatta agaaaaatag caataaaaat
     3001 aagatattca
//
LOCUS       DACEPW010000240         2993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEPW010000240 DACEPW010000000
VERSION     DACEPW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.246"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..570
                     /locus_tag="IW819_002589"
     CDS             100..570
                     /locus_tag="IW819_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1245671.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IW819_002590"
     CDS             792..1895
                     /locus_tag="IW819_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1245672.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2116..>2993
                     /locus_tag="IW819_002591"
     CDS             2116..>2993
                     /locus_tag="IW819_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1245673.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIP"
BASE COUNT         1036 a          505 c          645 g          807 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact tcgccgattg taaaattaag
     2041 ctagacaact aaaaaaagtc atttgtgcta cactcaaaat agttccgacg aaagaattat
     2101 aaggagtgag tacaaatgac ctataaccat cttacaccaa ctgaactcgt aatgatagaa
     2161 gcttatttca atcaatcaca acctgtttca aaagtagcca atctgttaca acgttctcga
     2221 caaactattt ataaggtcta tcgttttctt aaatctggtg gaagcgcaat gagctactat
     2281 aaacaatata agaaaaacaa acgtaactgc ggaagacatc cgattgtttt acctgatgag
     2341 caacaggaat acattcaaaa aaaggttgtt caagggtggg ctcctgatgt ccttattggt
     2401 cgagcagagt ttcctattgc ttgttcggta cgcacactgt atcgaatgtt taaagataaa
     2461 acctttgaca ctcacgattt accaatgaag ggaaaaagaa aaccaaacgg tcataaagaa
     2521 aaaagaggaa aacaagggtt tcgccggtca atccgagaca gaaagacaga ctatacccag
     2581 tttgatcatg aatttggtca cctggaaggt gacactatcg tgggtggaaa acataaaagc
     2641 gcggtcatca cattagttga acggctatct aaagtcatca ttaccttgaa acccgaaggc
     2701 agacaggcta aagacattga aaataggctg aatcaatggt ttcgttgtgt cccaaaaaac
     2761 ttatttaaat ccatcacatt tgattgtgga aaagaattct caaattggaa aaagattagt
     2821 aataccaacg atatttcaat ttattttgcg gatccaggaa caccttctca acgaggattg
     2881 aatgaacatt caaatggctt attacgtaaa gatggtttgt ataaagctat ggattttaat
     2941 ggtgtatctg aaacctttat ccaatcggtg gcatcgaaac gaaatcatat tcc
//
LOCUS       DACEPW010000241         2981 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEPW010000241 DACEPW010000000
VERSION     DACEPW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.247"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..549
                     /locus_tag="IW819_002592"
     CDS             <1..549
                     /locus_tag="IW819_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1245674.1"
                     /translation="DDKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKK
                     IIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIV
                     EHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDI
                     PSHSLAYGNPAKIKKEGINFEF"
     gene            539..1681
                     /locus_tag="IW819_002593"
     CDS             539..1681
                     /locus_tag="IW819_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ1245675.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1694..2671
                     /locus_tag="IW819_002594"
     CDS             1694..2671
                     /locus_tag="IW819_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1245676.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            2671..>2981
                     /locus_tag="IW819_002595"
     CDS             2671..>2981
                     /locus_tag="IW819_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1245677.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     N"
BASE COUNT         1092 a          370 c          576 g          943 t
ORIGIN      
        1 gatgataagc aaaataaaat gcaagatctg aaatatcttg ggaaaataaa cgaattgaat
       61 agctgggcgg atagcaaaaa aaaaatctat aaatttattg tagctattgg tgactgtgta
      121 gtaagaaaaa agattataaa aaaattgaat atgccaagtg agcaatatgg aatagctgtt
      181 catccaagtg ctatagtggc aaaagaaatt tcaattggtt atggaactgt tatttttgcg
      241 aatgctgtaa ttaacccaga tgctgtaatt ggagagcatg ctataataaa cacaggtagc
      301 attgttgagc atgattgtag aattaataac tatgttcatt tatctccagg tgtatgctta
      361 gcgggtggag tacatgtagg tgtaggaact caaataggaa ttggttctca atgtatccag
      421 atgaagaaaa ttggatcttg gtgtatgata ggcgcaggga gcactattgt taaggatatt
      481 ccctcacatt ccttagctta tggcaatcca gctaaaatta agaaagaagg aattaatttt
      541 gaattctaga attttattat caagtcctca tatgggagga acagaagaaa aatatgttgc
      601 taaggctttt aaagataatt ggatagcgcc attaggtgaa aatgtggata aatttgaaga
      661 aagtattaca aaatatactg gttctaaggg cggagcttct gcgttaagtt caggtacagc
      721 tgccatacat cttgccttaa gactgcttaa tgttggaaaa aacgatattg tctttgctcc
      781 ttcatttact tttatagcta cagtaaatcc tattttatat caaggggctg taccagtgtt
      841 cattgactca gaacctaata catggaatat gagccctgtt gcactagaga gcgcattaga
      901 agaagctgaa agaaacaacc aattacctaa ggcaatcatc gtagtgaatt tatatggtca
      961 aatggctgat tatgaacgaa ttttagctat tgcaaaccaa tttaatgttc cagtgataga
     1021 agatgcagcg gaatcgttag gttctactta taagggaatt tcgtcaggct tatttgggac
     1081 aattgggatc tattcattta acggtaataa gatcatcaca acctcaggtg gaggagctct
     1141 agtgtctagt gataaaaaat atgttgagca ttcaagattt ttagcaacac aagcaagaga
     1201 tcaagcaccg tattatcaac atagcgaaat aggttataat taccgtctaa gcaatatttc
     1261 tgccggtatc ggacgtggac aaatggaagt attgaatgat agagtagcta aacgaagaga
     1321 aattttcaat cgatataagg aagctttttc tggaattgat agaattgaaa tgatgccaga
     1381 agtggatggc atggtgccaa atcgttggtt gtctacttta acgatcaatg attataattc
     1441 tattgatcaa gtagttaacg taataaataa actaaatgaa aaaaacattg aaacaagagc
     1501 tttgtggaaa ccactacatt tgcaaccagt atttcaaggg gtaaaaatgt attaccatga
     1561 taataatctt agtgtgtcag aaaagttatt taatagcggc ttatgcttac cttctggaag
     1621 taatatgaca gatagtgaac aagaatttgt tattgatgag tttaaaaaaa tattcaagta
     1681 ggtgtgatat tttatgaaca taggaattat tggtttaggt tccatgggga aaagaagact
     1741 tagactcatt caagaacact ttaatgatat tttactatgt ggtgttgata ctaatgaggc
     1801 gagacgaaag gaagttgaaa aggaatttgg aataccaact tataatagtc taacagaggc
     1861 aaaaaatttg tttgaaataa attcagtttt tgtttgtaca tcccctataa cccatgaatt
     1921 tattataaaa gaggctttaa atttagattg taatatcttt acggaaatca atttattaaa
     1981 tgaatattat gatgaaataa tcgaagtagc taaaaagaaa gatagattac tttatctatc
     2041 ttctacattt ttgaagagaa aagaaattca atatattaag tctaaagtga cgcataataa
     2101 aaaaatttct tatcaatacc atgtaggaca gtatttaccc gactggcatc cttgggaaga
     2161 ttatcgcaat ttttttgttt caaacaaaaa aactaatggg tgtagagaaa tattcgctat
     2221 tgaattacct tggattatag atacatttgg gaaagttaaa tcgtttactg taatgaccca
     2281 gaaattatcc aatcttgata ttgattattc ggacagtttc actcttatta ttgagcatga
     2341 aactggtata gttggaactt taagtgtaga tgttgtttca agagtagcta aaagaaattt
     2401 aactataatt ggtgaaaaga ttttaataga atgggaaggt aacccggatt ctctattttc
     2461 atggaatgaa gaaacttcta gtatgacaga tattcaattg tacgatgagg ttatgcatga
     2521 taataattat tcacatacta ttattgaaga tgcatattta gaggagatta aagaattttt
     2581 cacgcttttg gaaaagaaaa tggaacccca atatactttt gaaaaagata agtatattat
     2641 taacttaatt aacgacattg aggtaaatta atgatgaaag taattttttt tgggttaggg
     2701 tcaattggaa agagacatct aaaaaatcta ataagctatg gaaaaaagaa ttctattaat
     2761 tttgatataa cagcttatag tagcagtgga aaaaaagagt ttgaagaaat aacgtatatt
     2821 caaaatagag acgatttgga tacacattat gatatagctt ttattacaaa tccgactttt
     2881 aagcatctgg aaacactaaa tgtaattgtt gaaaaagttg attacatatt tttagaaaaa
     2941 cctgcttttt ctgagagtat tgatcttact caatataatt a
//
LOCUS       DACEPW010000242         2969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEPW010000242 DACEPW010000000
VERSION     DACEPW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.248"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(88..1035)
                     /locus_tag="IW819_002596"
     CDS             complement(88..1035)
                     /locus_tag="IW819_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1245678.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1281..1745
                     /locus_tag="IW819_002597"
     CDS             1281..1745
                     /locus_tag="IW819_002597"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1245679.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1807..2850)
                     /locus_tag="IW819_002598"
     CDS             complement(1807..2850)
                     /locus_tag="IW819_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1245680.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLFYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          983 a          588 c          564 g          834 t
ORIGIN      
        1 accatttatt cggaaaaaag gactgtgaca agacttttgt cacagtcctt tttcatttaa
       61 aaaaatgttt acttcatttg acgtatatta ttggtgaggc ttcaattcat ttggcttcac
      121 tttttctgtt tctcttatga tataaacagg acgctgtttc gtttctaaga agattttccc
      181 gatatatttc ccgatgattc caagacataa caattggatt ccaccaatga acaggaagat
      241 cgagaccatg gaaggccatc ctgatgtagg atcaccaaac aacaatgccc gcaaaataat
      301 gaacaaaatt gcaataccag aaccgacaca agacaaagcg ccgataaatg aagcaatcgt
      361 taacggtgca tccgagaagt tcacgatacc atcaatcgag tagttgaata atttccagaa
      421 ggaccaagat gtttcgccag ccacacgttc gcggttttca taagaaatat attctgtttt
      481 aaacccgacc cagctaaata atccttttga aaatcggttg tattctgtta attctaggac
      541 ggcatccacc atttgacgag tcatcaagcg gaaatcacga gcgccatcga ccatttctgt
      601 atcgctgatt ttattgatca actggtagaa ttttttcgca aagaaactgc gtatcgcagg
      661 ctcgccttca cgagtcgaac gccgggtgcc gacacaatcg atatcagaag tagtgatccg
      721 gttgatcatc tcaggaagca attcgggcgg atcttgcaga tcaacatcca tcacggtcac
      781 taaatctcct gtagaagctt gcaatccagc gtatagagcc gcttctttcc caaaatttct
      841 ggaaaatgag acaaagcgga cataaggata agctgaggct agcttcttga ttgctgctaa
      901 cgtaccgtct tttgatccgt cgtcaataaa caaatattcg aactcatggg cacctttgaa
      961 tttttcaacc tcatcaaaaa aaagaggaac agttttttct tcattaaaac atgggacaac
     1021 aattgatact ttcatcgttt ctatcctttc aatcgtatgt ttatatatgg tttacattca
     1081 aaatttaata aaccttaaat aacaccttta aaatttgatt ttacgtaaaa gaacaggtat
     1141 tctttttgta aactaacgta tttagtttac catgtttcct cggtttatca aacttttaag
     1201 aacaattttt attagttggc aagtagtttt ggatagagaa aaaggaggaa gttccagtct
     1261 attgactaga agcaaacaaa atgaaaatac acacacctat cggcttttta tggttagatg
     1321 cagatgaatc cggattgact gctgtatctt ttcagccaat ttcagaaagc gaatcagcga
     1381 atcagcaaat tttaacgcgc acacaagaac agtttgaaga atattttgcg ggaaaaagaa
     1441 aagaatttga tgttccttta tctataaaga gaggtacgcc ttttcagcaa aatgtctgga
     1501 aagctttgtc gaccattcct tatggagaaa tccgaacgta taaagaagta gccgttgctg
     1561 ttgatagtcc gaaagctgtg cgagcgattg ggcaggcaaa tcgaaccaat ccattgccaa
     1621 tcgttattcc ttgtcatcga gtgatcgggc aaaatggaaa actgacagga tatatgggaa
     1681 acgcaataga tggggttgaa atcaaacgcc aattgttgtt gttagaagga tatctgcaaa
     1741 attaaaaaca gttgtgattc ggacattgtt ccatgcgaga atcacaactg ttttttcatg
     1801 ttcattctaa actttgatca gccaaataaa tagtaaagcc atcactgttt ggagaagccc
     1861 gacagctaat ccataaaata agaagcgtgt accttctttc atgaattttg caaagtctaa
     1921 acgcagacca attgccgcta aagcagtgat ctcaaaccag ctgctgataa aatgtgccga
     1981 ttggtcaata actacaggta aagaaaacaa gctgttgacc aaacaaaaga tcacaaaccc
     2041 taatacatac caaggaagtg gtaattttcg ttttttctgc gtatcagttg gcgtttcaaa
     2101 cagagctggt tgtttcttgt gcatgaattt ttcaaacaga tagacaacaa caacgagcat
     2161 cataatacgt agaatcttaa atagcatggc gaattcgaca acagaattgt caagtaagct
     2221 tgccccagcg actacttgac caacagattg gagtgtgcca cccaaaagtg cacttttttc
     2281 aatgagattt ccaccaaaca actgtaatcc aagaaaaggg agagtcaaca tcatcacagt
     2341 acccagtaga ttgactagtg tgatgatctg tcctttttct tcctctttcg catggatgga
     2401 aggggcgatc gaagcaatag cagaagaacc acaaacagcg tttcctccag ccatcatcaa
     2461 agaagaagta tcgctgaatt gcagtttttt accaatcaag taagcaccga tgatagtgac
     2521 tgacatttgc agtaacacaa aaacaattcc ttttatacca atttgagcaa tcgtttgagc
     2581 tgtgacagtt gcacctagta aaaaaacaga gaattctaat agtttgcttt cagcaaattt
     2641 tgtgccgatt gctagaacag gctgtttaaa aaaagtattt cctaataaaa tacccaataa
     2701 aatagcaata gtcgctccac ctaactgtgg cagataaata gcaagaagcc tgctgaaggc
     2761 agcgatggca aaagaaagca gtaaaccggg taagatttcg cggaccgctg aatcctttac
     2821 agattttatt ggatgtgaca atgatttcat ttgatcggtt cctttcgtca ataaatacta
     2881 aaagcatact cgaacatagt agcactaaac cagtattttg acaaaagaac aagaggtcgt
     2941 gacaaaagtc ttgtcacgac ctcttgttt
//
LOCUS       DACEPW010000243         2938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.249, whole genome shotgun sequence.
ACCESSION   DACEPW010000243 DACEPW010000000
VERSION     DACEPW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.249"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1380..1898)
                     /locus_tag="IW819_002599"
     CDS             complement(1380..1898)
                     /locus_tag="IW819_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1245681.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          969 a          531 c          422 g         1016 t
ORIGIN      
        1 tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc ttactgtctt
       61 ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat acttctgcta
      121 agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc cttgccataa
      181 taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc attttagtac
      241 atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag aaaaaaactt
      301 attaaaccag atataacttg taaacatcca actatataga aaacgtaaat tttctatata
      361 gttggattaa gataacgact atttatttta tcatattttt actataccct atacttttac
      421 ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt
      481 aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat
      541 ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt
      601 gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga
      661 aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat aaaaactaat
      721 gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt
      781 caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa
      841 taggataggc gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta
      901 tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt
      961 gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga
     1021 cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact
     1081 atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag
     1141 tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc
     1201 tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag
     1261 tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt
     1321 tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt
     1381 tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact
     1441 gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc
     1501 cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta
     1561 atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag
     1621 gtatttgtaa accacttata taactctcct aactgattcc atttaaattg atcagaaggc
     1681 gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa
     1741 ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc
     1801 ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt
     1861 tcttttttta attcttcttt atttttatat gttctcatta tattacctcc ataaattcct
     1921 aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg
     1981 catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac
     2041 gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt
     2101 ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta
     2161 actactatca attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt
     2221 taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa
     2281 tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt
     2341 atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa
     2401 aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc
     2461 tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg
     2521 aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc
     2581 ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta
     2641 ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt aagggatcaa
     2701 gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa acaattatag
     2761 aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact ttcttattct
     2821 gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta cgagtactat
     2881 tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc ccacttag
//
LOCUS       DACEPW010000244         2933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.250, whole genome shotgun sequence.
ACCESSION   DACEPW010000244 DACEPW010000000
VERSION     DACEPW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.250"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..454
                     /locus_tag="IW819_002600"
     CDS             <1..454
                     /locus_tag="IW819_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1245682.1"
                     /translation="DLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGK
                     EFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLE
                     LFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
     gene            555..1127
                     /locus_tag="IW819_002601"
     CDS             555..1127
                     /locus_tag="IW819_002601"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1245683.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGW
                     CYLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAR
                     EVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYI
                     EGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1600..2172)
                     /locus_tag="IW819_002602"
     CDS             complement(1600..2172)
                     /locus_tag="IW819_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1245684.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2188..2793)
                     /locus_tag="IW819_002603"
     CDS             complement(2188..2793)
                     /locus_tag="IW819_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ1245685.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          920 a          581 c          481 g          951 t
ORIGIN      
        1 ggatctgttc aatcgagaaa ttctcggcta ttcttgtgga gaacataaag atgccgttct
       61 agtaaaaaaa gcatttagcc gtatcaaaca acctctgaca gaggttgaga tttttcatac
      121 tgatcgtgga aaagagtttg ataaccaagc tattgatgaa ttattaacaa cttttgacat
      181 caatcgatca ttgagtcata aaggctgtcc ttttgataat gccgtagctg aatcaactta
      241 taagtcgttg aaagtagaat ttgtctatca atacacattt gaaaccttac aacaattgga
      301 tttggagtta tttgactatg tcaattggtg gaaccacctt cggttgcacg gtacacttgg
      361 ctacgagaca ccggttggtt accgtaacca gagattggcg cagcgaatcc ttgataatga
      421 gctcggatgt gctaacgcta gcgaggcagt ctaactttaa ctgttagctc ctgccgaaga
      481 tcgtcacatc cgaggaggct cattgtcaag gacaatcgga atagcatacg gaaagaagtt
      541 ctgcacctta taaaatttgt caaaaaaact gttgccattc catcatttca aaagagaatc
      601 tcttaaatca ggacttttct actgaaacga tctgtgagaa atgggcagct gacattacgt
      661 acattcctac taagaaaaat ggttggtgtt acttatcttc aatcatggat ttacacacga
      721 aaagaattat tagttataca ttttcaaaac gaatgactgt ggattgtgtc attcaaacat
      781 tgaataaagc aaaaatacat tatcacattc cagaaggaat gattctacac actgacttgg
      841 gcagccaata cacagcaaga gaagtggaac aatggcttaa aaccaacaaa ataaggcatt
      901 cttatagtag aaaaggaaca ccttatgata atgctggaat cgaatctttc catgcctcat
      961 tgaaaaaaga agaagtctac acgactagct actcagattt tgaagaagca aatcgagcgc
     1021 tatttagcta cattgaagga ttttataacc gaaatcgaat ccatagctcg attcactatc
     1081 taaccccaca ggaatttgaa gaattagcaa aagaaaaaat ggcttaaccg tctctactaa
     1141 aatgtgtcca agatattgac tcaaatccag taatatcaca ataggaatgc tgtttattat
     1201 attagcagca gtgctgattt ttatgatagt tatttggttc ttaaatgaaa gatctaaaga
     1261 caatcaacac cgtagggata tggagataaa atcaatagag tcaatagatt atttatcgat
     1321 aataacacta aaaaagccac gttcctaaac gtgacttacg ctgaacgata tgtgttgttt
     1381 ggtagacgaa caaatcgttc attataaata tatgatggta tcctaatata gtaagcgtgg
     1441 ctagcatacc tttaattact ttacctttta cagttaccat acacattttt tgagcagaaa
     1501 tcaagtaaat aaaaaaatat tatatttcac aacatgctaa tagactcata tctagaacgc
     1561 attaagtatt tacctgtctg gttcgctgag acttcttttt taactttctt caattttctt
     1621 ctttattcta aaaagcgtac tgcgactgaa accggttata gcttcaactt ccttatacga
     1681 tttacctgaa gtaagcagtt catatgcgtg cctcttcttc ggtgtgattg ttgctttcgg
     1741 acgtccttct ctgaaattag gattattctt tcttgcaaac atttttcctt cttgggtacg
     1801 ttccacaatc atatcacgtt ccatttctgc tacactcaac aacgtccgtg tcaccattcg
     1861 ccccatggac gtattttcaa tcgttcctag attcaaaacc ttgatcgtta cgttatcatc
     1921 gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta cgagcaaatc gatcaagctt
     1981 cgtaacaact atttcatctc caggcttcag accagccaac agctgattga attttggccg
     2041 atcccgtttt gtacccgtaa atttttctga aaaaatattc ttcttctcta cgccgttctc
     2101 aagcaactgt tgaatttgtt cttttgtact ttgagaagcg gttgagacac gtgcatatcc
     2161 atatttcgtc atttggtcca cctactttta caaatcatct atcgtatact cagaataccc
     2221 ttcatagaaa tagctgacat cgattccttt catatataac tcgcgatcgt caattttatc
     2281 agtcagagca ttggaaatta gatagcgtat ttccaaatcg tttatgggac ttcgctccat
     2341 tgcagaaaga tagtctgctt tatcgattaa gttccaatct acaacttttt gcaattcttc
     2401 ctttaaaatg agatccagcc aaattctagt acttcggcca tttccttctc gaaatgggtg
     2461 agcgatattc atctccacat atttcttgat aatctcttca aaagaagttt gtggcatctg
     2521 atcaatatgc tgtaacgaat gttctaaata cataactggt gcaaatcgaa aatttccttt
     2581 tgcaatattg accgatcgga tttttccggc aaaatcgtaa atatccgaaa atagaaattc
     2641 atgaatttca gataaacctt taaatgtacc aacttcgatt tcatttattt ttccacaatc
     2701 gtacaattct ttcgcttttt gtttactgag tttttcttca accttagcca actcaacttg
     2761 attggttaat cccaatttat tctctaaaat catgcgaaat ctccttaact ttatgacata
     2821 agtttatgaa acactctcaa ccccattata tcgcgatttt gtttatgtgt caaaaactat
     2881 taagtttctg acccccgata gatttttcaa gtgtctctat tttgcacagt cta
//
LOCUS       DACEPW010000245         2918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.251, whole genome shotgun sequence.
ACCESSION   DACEPW010000245 DACEPW010000000
VERSION     DACEPW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.251"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            205..1173
                     /locus_tag="IW819_002604"
     CDS             205..1173
                     /locus_tag="IW819_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1245686.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1166..2197
                     /gene="vanA"
                     /locus_tag="IW819_002605"
     CDS             1166..2197
                     /gene="vanA"
                     /locus_tag="IW819_002605"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1245687.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2203..2811
                     /locus_tag="IW819_002606"
     CDS             2203..2811
                     /locus_tag="IW819_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1245688.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          708 g          788 t
ORIGIN      
        1 taatttttta ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa
       61 tattaagaaa cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc
      121 gttcttcaga gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt
      181 tcacagaaag ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag
      241 caggatgagg cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata
      301 attaacgcca acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt
      361 gtgggacata aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg
      421 aaatatattt ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga
      481 atgggcatca ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg
      541 atgctaattc ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat
      601 gatttcaggt tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg
      661 ggaacgggcc agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg
      721 ttggcttata gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg
      781 caaaatagcg atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc
      841 agccacgaac aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt
      901 ccacttgtag atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc
      961 gcattggatg tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca
     1021 attgataatc aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat
     1081 acggcctatt ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt
     1141 ttggattttg aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg
     1201 ttgctcagag gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa
     1261 taaagaaaaa tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg
     1321 cgaaaaacct tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga
     1381 taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga
     1441 tgtagcattt tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt
     1501 tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga
     1561 caaatcgttg acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt
     1621 tattaataaa gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc
     1681 ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta
     1741 cgcaattgaa tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg
     1801 ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga
     1861 ccaaatcagg ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg
     1921 ctctgaaaac gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat
     1981 acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga
     2041 tatgttttta caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt
     2101 cacgtcatac agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact
     2161 gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact
     2221 tttttagatg aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat
     2281 ttcaccggaa aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg
     2341 gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta
     2401 tgggacggtt accgtcctaa tcgtgctgta aactgtttta tgcaatgggc tgcacagccg
     2461 gaaaataacc tgacaaagga aagttattat cccaatattg accgaactga gatgatttca
     2521 aaaggatacg tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt
     2581 tatcgattag acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa
     2641 cgctctcatc atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg
     2701 cgctccatca tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta
     2761 ttaagagacg aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac
     2821 cgttgcacgg acaaactata taagctaact ctttcggcag gaaacccgac gtatgtaact
     2881 ggttcttagg gaatttatat atagtagata gtattgaa
//
LOCUS       DACEPW010000246         2911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEPW010000246 DACEPW010000000
VERSION     DACEPW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.252"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            474..569
                     /locus_tag="IW819_002607"
     CDS             474..569
                     /locus_tag="IW819_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1245689.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            1270..1554
                     /locus_tag="IW819_002608"
     CDS             1270..1554
                     /locus_tag="IW819_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245690.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1557..1973
                     /locus_tag="IW819_002609"
     CDS             1557..1973
                     /locus_tag="IW819_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245691.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2110..2829)
                     /locus_tag="IW819_002610"
     CDS             complement(2110..2829)
                     /locus_tag="IW819_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1245692.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
BASE COUNT          969 a          555 c          461 g          926 t
ORIGIN      
        1 cttctgtatc cgttgggaga gtaaggctca actgccccct aaaaagggtt ctaaaaaatc
       61 tttaaaaaat aaataaaata aacgcgcgcg cgcgagcaaa atttcccgcc aaaaaatcac
      121 ccacttttta aaataaacag gttcgcgcat gatattaaac tgccaatcta gctccctatg
      181 gtcgctgtaa gaaggagtga tcgctacgct ctcacatttt acttgcagca gtcgccttta
      241 ggctccttcg ccaaaaataa ttcattaggg actcctttgt ggcgtcctga gagctcgtct
      301 aagccgttct aagacaagat tcgataaaaa ctatgtcctt cgttctaaag cgccttacag
      361 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
      421 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
      481 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
      541 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
      601 aagccccaga gaatccgacc tctctgggga tttcgtgtgt ttcgtgttca cttatttgca
      661 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
      721 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
      781 gatttaagac gttttattta gtttttggtg tatttcattg gtccaagcta aaaaaactct
      841 tagaagcgat tttagagcca tttaaaccca cttaattata ttctccatga aattcgctcc
      901 atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt cactagttgc attaattttt
      961 ccaacgatcg ttttctttcg ggtgtcatat ttttatattc caacaatttt tcgtctgtta
     1021 aatttggtta tcgattacac gtgcagcact cccccccgaa ttttaaagag cataaggaaa
     1081 cgtgtaccca ctttctaaaa aaagtgggta gataggctgt gcctatcacg tttctcagaa
     1141 aaaaatttga tatcactttt tcgccatcgt gggtagctta tgtatagcat tttggcaaaa
     1201 aacagctata caaatatagt atttgtatgg tatacataaa ttatacataa ttgcaaaagg
     1261 agatttttta tggacatttt agttcggaat attgatccaa agtttgttgc aacaatagac
     1321 aaacgttgcc aagaactcac gaataaaaca ggagaaaaat ggagtcgaaa taaatatcta
     1381 aaagttttaa tcgaaagtga ttttgaccgt caacttacga cttacaaaaa agaccaattc
     1441 gatttattgc tcgataaatt tattgctatc caaacagaaa atactcaaat attaagcgag
     1501 tatgtctaca caaataatca aatcattgaa atgcttatcg gaggaatgat ataaccatga
     1561 gaaaaacttc tgtccaaaaa catgttactt tgtaccctag tcatgttgac gttttagatt
     1621 ccatcatcga aaaaaatcca gatatcaata actactcgga agccattcgc tttttatgta
     1681 tgagttatga acccgatacc aacacgacca ctaccagcaa tcaggcaaaa ctaaatgcta
     1741 tgggaaaaga aatttccatc atgactgaaa tcatcgcaga atttgctgat ttatatctaa
     1801 aagatacagg cgttcttatt ggcacaaatt ctaaagtata tcaagaagtg aaagaaaaaa
     1861 tcgaagctaa actaaatcca tcaaatcgaa atcgaccaaa gaatcatcaa aaagaaacca
     1921 gtcaaacagt cacttctaga aatttcgacc caacaaattt tctaaaacga taaaaaacag
     1981 ataggagtta ttcctatctg ttttttattt agaagcccca tacaatatgc tagaatgtac
     2041 tagcaatctt agtcggttgt aagggacttg tctacttgct taatgtaagg ggggcgagtt
     2101 cttttttatt tattcttcta accagttacg cagagaaatc tttggcaaat ctgacgttag
     2161 gcttgaaaaa gtaaattcta atttggcgat tttattgccc ttttttgctt ttatttttgt
     2221 gactttaaga tcattgaagt atttttttag ttctcgtaat gcaggattta atactttttg
     2281 atcaatgtta cccatttgat agtattctgg gatatccatt aagctcctaa actcttcaat
     2341 acgaactaca taataaccag tagaacggta ctgcataagc agtcggaata gagtttttgc
     2401 atacgtagaa cgcaacgacg tgaaagcaga taattcaaat ctactaaatt cagctgttaa
     2461 accattaata atatattcta ggtccggatt aacactgact tcaacaaact tttttgtttt
     2521 attgatttca aaaccagtaa acaaaacaaa gtagcgaata gaatcctcat cttcagtacg
     2581 gtaagtaaga ttaagcatat ctttatagag cttttctaaa tcaccgacaa aggcttttgt
     2641 agcagtcatc ttataatcac ttaactcttt taattcctcg aaagaaaatc taactttttt
     2701 caaaccacga tctttcatac gagcgcataa tgcaaaaaat aaatccatct gcttagcatt
     2761 gaaatttttc agcggtacta aattcaattc attttgatat ttgactgtta attccgtttt
     2821 ttcatccatt cctttcttcc ccccttacaa ataaataata acctttaaca accaactcgt
     2881 aaataggttg gttgttatta tacaaaagga t
//
LOCUS       DACEPW010000247         2898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.253, whole genome shotgun sequence.
ACCESSION   DACEPW010000247 DACEPW010000000
VERSION     DACEPW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.253"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            94..996
                     /locus_tag="IW819_002611"
     CDS             94..996
                     /locus_tag="IW819_002611"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1245693.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            1019..2425
                     /gene="atpD"
                     /locus_tag="IW819_002612"
     CDS             1019..2425
                     /gene="atpD"
                     /locus_tag="IW819_002612"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1245694.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            2439..2861
                     /locus_tag="IW819_002613"
     CDS             2439..2861
                     /locus_tag="IW819_002613"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1245695.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT          944 a          555 c          649 g          750 t
ORIGIN      
        1 aatataaaga catgttctta gctacaaaag gaaataattc ctctacagaa gataaattga
       61 aatccattca aaatgcatag tgaggtgaga tgaatgggtg cttcattaaa tgaaatcaag
      121 acacgtatcg cttcaacgaa aaaaacgagc caaatcacgc gggcaatgca gatggtgtct
      181 gcttctaagt tgacaaaatc tgaagcttct tcacagaagt ttcaggttta tgcaacaaaa
      241 gtaagagaaa tcgttacgca tttgactgct actcagttaa atgatattgc ctcagaaaat
      301 cctagagggg atatcaatta caacagcatg ctgctcagcc gtcctgtgaa gaaaaccggt
      361 tatatcgtca tcactgcaga tggcggtctg gtcggtggat ataacagttc gatattgaaa
      421 caaacgatgt ctattttaga agaagaccac caatcacccg atgattacgt gatgatcgca
      481 attggcggaa ctggtgcaga cttctttaaa gcacgcggta tcaacctagc atatgagctg
      541 cgcaatttat ctgatcagcc aagctttgat gaagtccgca aaatcgtaaa tatggcaaca
      601 actatgtacc aaaacgaagt attcgatgaa ttatacgtat gttataacca tcatatcaat
      661 tcgctaacta gtcagtttcg tgtagaaaaa atgctgccaa tctcagactt agatccagaa
      721 gaagcaacga ctttcgaaca agaatatatt tttgaacctt caaaagaaga aatcttgtct
      781 cagttattgc ctcagtatgc tgaaagtttg atctacggtg cgatcgttga cgccaaaaca
      841 gcagaacatg ctgctggtat gacagcaatg aaaacagcaa ctgataatgc tgctaatatc
      901 attgatgagc tgacggtttc ttataaccgt gcaagacaag gggctattac ccaagaaatt
      961 accgaaattg ttgccggagc ttcggcacta gaatagtatt gagattggag gaaaaaagat
     1021 gagttcaggc aagattgttc aagtaatcgg tcccgttgtc gacgtggaat tttctttaga
     1081 ccaatcctta ccagatatca acaacgcatt agttgtttat aaaaatgacg aaaataaatc
     1141 aaaagttgtt cttgaagcag cgttagaatt aggggacgga gtgatccgga ccattgcgat
     1201 ggaatcaaca gatggtttac aaagaggaat ggaagtcatt gatacaggca aagcaatctc
     1261 tgttcctgta ggtaaagaaa cattaggtcg cgtattcaac gtactaggag atacgatcga
     1321 tttagaaaca cctttcccag aagatgcgga aagaagcgaa attcataaaa aagcaccagc
     1381 ctttgacgaa ttaagtacaa gtacagaaat tttggaaaca gggatcaaag ttatcgattt
     1441 gcttgcccca tatttaaaag gtgggaaagt cggactattc ggtggtgccg gtgttggtaa
     1501 aaccgtactg atccaagaac tgatccataa tatcgcccaa gaacatggtg gtatttctgt
     1561 atttaccggt gtaggtgaac gtactcgtga aggtaatgac ttgtattatg aaatgaaaga
     1621 ttcaggagtt atcgaaaaaa cagccatggt gttcggacaa atgaacgaac caccaggtgc
     1681 acgtatgcgt gttgctttga ctggtttgac gattgcggaa tatttccgtg atgtagaagg
     1741 tcaagatgta ctgttgttta tcgacaacat tttccgtttc actcaagctg gatctgaagt
     1801 atcagccttg ttaggacgga tgccttctgc ggttggttat caaccaacat tggcaacaga
     1861 aatgggtcaa ttgcaagaac gtatcacatc tacgaaaaaa ggttcgatca catcgatcca
     1921 agcaatctat gtgccagccg atgactacac tgacccggcg ccggcaacag cattcgccca
     1981 tttggatgcg acaaccaact tggaacgtaa attaacagaa caagggattt atccagcggt
     2041 agatccattg gcttcttctt caagtgcttt agcacctgaa atcgtgggag aagaacatta
     2101 taaagtagca acagaagtcc aacacgtatt gcaacgatat cgtgaattac aagatatcat
     2161 tgctatctta ggtatggatg aattatccga tcaagaaaaa gtgttagttt ctcgtgcccg
     2221 cagagtccaa ttcttcttgt cacaaaactt taacgtggct gaacaattta ctggtcttcc
     2281 aggttcatat gtccctgtag aagaaacagt gaaaggattc cgcgaaatct tggaaggcaa
     2341 atacgatgat cttcctgaag aagctttccg aagtgttggc cgaatcgaag acgtcgtaga
     2401 aaaagcgaaa acactaggct actagaaagg ggtgccccat ggatcaatat ttaacagtaa
     2461 atgtggtaac tcctgccggt ttggtctatg atcaccatgc agctatcgtc gttgcacgta
     2521 cgacagctgg tgagcttggg attttgccta aacatgcacc aatcatcgtt ccattgacga
     2581 ttgatgaagt tcgtgtaaaa cgaacagatt cagataccca cgtcgattgg atcgctgtta
     2641 atggcggaat catggaagtt cgtgataatg ttgtttcaat cgttgctgat agtgcggaac
     2701 gtgaacgtga tatcgatgta tctcgtgcgg aacgtgcaaa acaacgcgca gaacgccaaa
     2761 tcgaagaagc aaaagcaaaa caagatacga acgaattgaa acgggcaact gttgcgttgc
     2821 accgagcaat caatcgtatc aaagtatcta agcactcata aaatgagatg cggatgttta
     2881 tccgtgttta agaacagt
//
LOCUS       DACEPW010000248         2895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.254, whole genome shotgun sequence.
ACCESSION   DACEPW010000248 DACEPW010000000
VERSION     DACEPW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.254"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(15..962)
                     /locus_tag="IW819_002614"
     CDS             complement(15..962)
                     /locus_tag="IW819_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1245696.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="IW819_002615"
     CDS             complement(1140..1943)
                     /locus_tag="IW819_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1245697.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..>2895
                     /locus_tag="IW819_002616"
     CDS             2089..>2895
                     /locus_tag="IW819_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1245698.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRII"
BASE COUNT          936 a          468 c          564 g          927 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatt
//
LOCUS       DACEPW010000249         2893 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.255, whole genome shotgun sequence.
ACCESSION   DACEPW010000249 DACEPW010000000
VERSION     DACEPW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.255"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2607
                     /locus_tag="IW819_002617"
     CDS             <1..2607
                     /locus_tag="IW819_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1245699.1"
                     /translation="ISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHG
                     NLFPATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAG
                     FAAARNMTIKVEGIEVAYSDPATDPPGQERLTRLIDPIYTVTSSNHSGNQDHTMSFRA
                     NADGLLTIKNQATGEVLENAKDLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEE
                     YVEMASYETKEILHTVDYRKPNYSTVYVTPEGSDTGNGSRQNPLSLTQALRYAYAGQT
                     IVLAPGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHY
                     WVLRGFDVTNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNL
                     VKNCTSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGK
                     VVIKNSVAYRNGWVPGVEGEGNGNGFKMGDSSLSGPHELINSLSFENLAKGIDSNSGT
                     DIIVNSSTSFNNGSYNVALYTSSAGNTDFHASGILSFRTENLEMREQIRPLNQNEDQI
                     YHSLNYYWNEREKQSENTLGHTVSKDWIESLNTHSQEGQQPVTRYANGSIHVHGLMQI
                     KPENRQTEKGRVDGLPLSVGATFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQE
                     ANGVAENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNH
                     TAIFSAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPER
                     ENPGNESPNGKPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLI
                     GVVVYVMKQRKK"
BASE COUNT          987 a          542 c          619 g          745 t
ORIGIN      
        1 atatcatctg aagatccgca agctgcgcca gctcctggga gtgtctcagt ggaaacaaca
       61 cctttagaga cttcagcagg gttcttggaa aaagaaacag gttcttataa tatattcgga
      121 aacggtcatg ggaatctgtt tccggccacg aactcgatta ctaaactgga cgtagagatg
      181 aaaaaaacga atactggttt agaggcatat tattacgatc ctgaaacagg agaagaaatg
      241 gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt cagatgaatc ggttatttat
      301 gcaggatttg ctgcagcaag gaatatgacg atcaaggtag aaggaatcga agttgcttac
      361 tcggatcctg cgacagatcc tcctgggcaa gaaagactta ctcgcttgat tgatccgatt
      421 tacactgtga cctctagtaa tcattcagga aaccaagacc atactatgtc ctttagagca
      481 aatgctgacg gattattaac aatcaaaaat caagcaacag gagaggtact tgagaacgca
      541 aaagacttgg caatcactgc aaatcgggaa tttgattatc agacaaaact gatggaagga
      601 acgaacgaat ttactttcag ttttacacca gataaaaact atccgccaga agaatacgta
      661 gagatggcta gttatgaaac aaaagagatt ctgcatacag ttgactaccg taaacctaac
      721 tattcgacag tttatgtaac accggaaggg tcagatacag gaaacggaag cagacaaaat
      781 ccgttatctc tgacgcaagc attgagatac gcatatgctg ggcagacaat cgtcctggct
      841 cctggaactt attcctttga aagaggattg acgattccaa aaggagttaa cgggacaaga
      901 gaaaacccga ttcaattgat tgcagagaaa aatccagaaa acaagcgacc tgtacttgat
      961 tttaaaggaa ctggaaatgg gatgacactg tttagtcatt actgggtttt acgtgggttt
     1021 gacgttacaa atagtgccgc tatgcaaaaa ggattgcaga tttctggaaa ccataatttg
     1081 attgaagaga tccatgctta tcggaatgga aatacaggaa tccaaatcag tggtagctca
     1141 aatgatcctt tcgaagcatg gccagcgcat aacttagtca agaactgtac atcttttgaa
     1201 aatgccgatc caggatttga agatgccgat ggatttgcgg ccaaactgac aataggagaa
     1261 gggaatgtat ttgacggttg tatcgcttat cataatgcag atgatggttg ggatcttttt
     1321 gctaaaaatg aaacaggttc aatcgggaaa gtagtcatca aaaactcagt tgcttatcga
     1381 aacggttggg taccgggagt tgaaggagaa gggaacggta atggattcaa aatgggtgat
     1441 tcctctttat cgggccctca tgaattgatc aatagtttgt ctttcgaaaa tcttgcaaaa
     1501 ggaatcgatt ccaatagcgg aacagatatt atcgtcaata gcagtacaag ttttaataac
     1561 ggttcatata atgtggctct ttatacctct tcggcaggaa acacagactt ccatgcttct
     1621 ggtatcttat ctttccgaac agaaaatctt gagatgagag agcagatccg cccattgaat
     1681 caaaacgaag atcaaatcta tcactcgttg aactactatt ggaatgaacg agagaaacaa
     1741 tcagaaaata ctcttggaca taccgtatca aaagattgga tcgaatctct aaatacacat
     1801 tcgcaagaag ggcagcagcc tgttactcgc tatgcgaatg gaagcatcca tgtccatgga
     1861 ttgatgcaaa tcaaaccgga gaatcgtcaa acagaaaagg gaagagtaga tggtcttcca
     1921 ctatctgtag gtgctacttt tgacggagaa acctcaccgt cttcggagta tccagaatat
     1981 gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac aaaataatcc gattattata
     2041 caagaagcaa atggtgtggc ggagaatgca gaaggaaata tcattgaact gaagcctttt
     2101 acagtttttg tgtcaacttt cacaccaaat caaggaacgc ctgtctttga agatatgcgt
     2161 tatttcccag aaaagatcca aatcgttgaa acacagtcaa gtgctcaatt ttctaatcat
     2221 accgctattt tttcagcacc ttcaaaagga gaatatacgc tagaagtcac ttatcgttcg
     2281 gaaatatttg atggggaaaa atggtctgct catacagaat ctgttaccca gcaaaaagcg
     2341 atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg acaaaccaga aagagaaaat
     2401 ccaggaaatg aatcaccaaa tggaaaacca aatggaaaac caaatggaaa accttctcat
     2461 ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc aaaaacggga
     2521 gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg tgtagttgtt
     2581 tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg taaaacaatt
     2641 gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact tccttctatc
     2701 tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta cttgatgcag
     2761 gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc tcggtattag
     2821 ctcaacaaac ggcaaaatat gaagaactat ttttccgttt ttttctctaa tactcaggtc
     2881 aggacgctga ttt
//
LOCUS       DACEPW010000250         2892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.256, whole genome shotgun sequence.
ACCESSION   DACEPW010000250 DACEPW010000000
VERSION     DACEPW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.256"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            166..2337
                     /locus_tag="IW819_002618"
     CDS             166..2337
                     /locus_tag="IW819_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1245700.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            2405..2746
                     /locus_tag="IW819_002619"
     CDS             2405..2746
                     /locus_tag="IW819_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1245701.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT         1022 a          529 c          649 g          692 t
ORIGIN      
        1 taaaacatat ttaagaaaaa acttacattt ttctgttcat tcttatgtta ctctacttgc
       61 actaacgtaa aaaaacatat tgaactacaa cttaagcagg aagaaaacac tctttcacta
      121 tggtaaaata agacgattga atcaaataga caggagaaga agcaaatgga ttttcaaagc
      181 attttagaaa agataaaagc tgctttgatc agtttttggc gttggatcag accatatctc
      241 ggacaattcc atcgatggcg aaagcgcatc tggaaaaaat atcatgtgaa caaattgatt
      301 ttgctgattg gtttgatctg tgtcttagtg acaagtatct acctctttat tttagcaaaa
      361 caagcaaatg tcgaaacgtt aaaatcagga ttaagccaat cgacagtcat ttatgataag
      421 aaaaacgaag aagctggcac actgtacgcc caaaaaggaa catatgtaga attaaacgcg
      481 atttctccct atatccaaga tgcagtaatc tcgacagaag ataggaattt ttacgagcat
      541 cacgggtttg atatcaaagg gattgcccgt gcagcagtcc gaatggtctt gtctggcgga
      601 acatcagggg gcggaggaag tacgatcact cagcagcttg ccaaaaatgc gtatctgaca
      661 ttagaccaaa cgtttgatcg aaaagcgaag gaattatttt tggcaatcga gatcgaaaag
      721 aagtacagca aagaagaaat cttgaccatg tatcttaaca atgcctattt tggcaacggg
      781 gtatggggtg tccaagacgc ttccagaaag tattttggag tagatgcaac gaatgttacg
      841 ctgtctgagg ctgctacttt ggcaggaatg ctgaaaggtc ctggtattta taatccgatc
      901 gatcacccaa aaaatgcgaa tgatcggaaa aacacagtac taagtgtaat ggaagaaaac
      961 ggaaaaatca caaaagaaca agaacaagca gaaagccaga cagatatcag cgcgtatctt
     1021 aatgacacat acgataattc agattctggt tatcgttatc cttattattt tgatgcagtg
     1081 atcgatgaag cagttaacga gtatggtctt gatgaagaag atgtcatgaa taaaggctat
     1141 aaaatctata cagctttaga tcaggattat caaaagcaat tagaagctac ttatcaaaac
     1201 agtgcattat tcccagcaaa tgcttcagat ggtgccatgg tccaatctgc ttcagtagct
     1261 ttagatccga atacaggtgg tgtacaagca ctagtaggac gaagaggaga tcatgtcttt
     1321 agaggattca gctttgctac acagatgcgt cgttcaccag gatcaacgat caaaccatta
     1381 agcgtatata caccagcact ggaagctgga tacaagcctt ccagtatctt gaaagacgaa
     1441 ccgcaaagtt attacgatgc gcataatttt gacgggacat accaaggaga agtaccgatg
     1501 tatcaagctg ttgcacaaag tttgaatttg cctgctgtat ggcttttgca tgagatcggt
     1561 ttacaaaaag gatatgataa agcagaagaa ttcggcctgc ctttagcaaa atctgataag
     1621 tattatggct tagcccttgg tggactggaa aaaggagtat ctccattgat catggcgggt
     1681 gcttacagtg catttgccaa tgatggtcag atgtatacgc cgcatcttat cacgaaaatc
     1741 gttgattcga ctggagcggt gatcgtcgat aacacaaaca aaaaaccaaa acaggtcata
     1801 tcaaaggaaa cagccgatga aatgaccagc atgcttcttg gtacattttc aaatggaaca
     1861 gcggctgctg cgaatcctgc aggttatacg atagcaggga aaacaggaac cacagaaact
     1921 aatttcgatg ccacaaaagc aaatgaccaa tggatgatcg gttacacgcc agacgtagtg
     1981 atttcgacat ggatgggtta cgaagaatcc agcaagctgc actatttgga agggacaagt
     2041 ggaaatgttg tcgggaaagt cttcaaatca gcggcagaag gcatccttcc gtatacttca
     2101 aaaacaggat ttagtgtagc agatgcctat gccacaggag gacaagtggt agctgcagat
     2161 caagttggaa acacaggaaa ttctaatgga gagtctggaa attggcaaga taacttgaat
     2221 caatatggtg aacaagcaaa agaagggctg aaaaacttcg gcgatatggt gaaagaaggc
     2281 gttcagggaa ttggtgaagc ggctaaagac ctctggcgga aatatcaagg agagtagccc
     2341 ctttgaaaac gaataacccc atgttacaat agagagtaac tactaaaaga aaagagggca
     2401 taacatgtca aacatctacg atagcgcgaa tcaaatcgaa cgtgagatcc gtgaattacc
     2461 agaattcaaa gcgttgaaag aagcgtacga gaaagtacaa gcaaacgaag aagcacacaa
     2521 actgttcaaa gacttccaag cgatgcaaat cgaattacaa gaaaaacaaa tgagtggaca
     2581 agagttcagc gacgaagatg caatgaaagc acaagaaatg gctgtgaaaa tccaatcaga
     2641 agaagtaatc aatgacttga tgcaaaaaga gcaaggcttc agtacaatca tcaatgactt
     2701 gaaccgtatc atcatgacac cagttcgcga cctttacagc gaataaaact attttatgaa
     2761 acaacagagg gtgtgaaaga gacgttcggc cttgagcgat gacaaaattg cttcttattg
     2821 ccgataggct gtctcttgaa caccatttac caagaggtcg tgagcctacc gtttaactct
     2881 gagttacaag ga
//
LOCUS       DACEPW010000251         2825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.257, whole genome shotgun sequence.
ACCESSION   DACEPW010000251 DACEPW010000000
VERSION     DACEPW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.257"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(131..697)
                     /locus_tag="IW819_002620"
     CDS             complement(131..697)
                     /locus_tag="IW819_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1245702.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(697..1653)
                     /locus_tag="IW819_002621"
     CDS             complement(697..1653)
                     /locus_tag="IW819_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1245703.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1653..2309)
                     /locus_tag="IW819_002622"
     CDS             complement(1653..2309)
                     /locus_tag="IW819_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1245704.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          887 a          564 c          534 g          840 t
ORIGIN      
        1 ttggttggac ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga
       61 aatcaacgct ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg
      121 cgttttttat ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt
      181 tgataaaccg ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat
      241 caaccatatc cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc
      301 gtccgcgagg cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca
      361 tagtgatgat tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga
      421 gattttgttg ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga
      481 cacgttcttc taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct
      541 ggatatcaaa actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat
      601 cgtggccatt gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca
      661 gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac
      721 atcatatttt ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac
      781 ttcccatttt ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt
      841 caaacgatgg atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac
      901 atagtcagga cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt
      961 tttgcgcatc agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt
     1021 gcgtctgaca ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg
     1081 cgtacaaaca cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata
     1141 atcatgtgct cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc
     1201 tacccataaa tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag
     1261 acaatccggt cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc
     1321 aaatcgttct ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac
     1381 gatgtattgt tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg
     1441 gattggaaga gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa
     1501 agtacaaccg ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat
     1561 cggcaccttg aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata
     1621 tcttttattt ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta
     1681 tctatttgtt ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg
     1741 agtaagtaac cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc
     1801 ccgacactta ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt
     1861 tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca
     1921 tgtccactag ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct
     1981 cgcatgaaaa atagtttgat caccgggttg atgattccag caattacaat aaatccagaa
     2041 acgaaccaaa atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca
     2101 aataaaatga tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc
     2161 agatcaggat aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac
     2221 gacagatgga actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca
     2281 gccacctgaa atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta
     2341 tagcattttt ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct
     2401 ttgaataacc aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa
     2461 tgcaaatgaa atgttaggga aataaaatca gtgtttttcg aaatcctttt aaacaagaac
     2521 gtagacgtag gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat
     2581 gattacaaaa aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa
     2641 ttattaataa tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag
     2701 aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg
     2761 tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc
     2821 aagcg
//
LOCUS       DACEPW010000252         2809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.258, whole genome shotgun sequence.
ACCESSION   DACEPW010000252 DACEPW010000000
VERSION     DACEPW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.258"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(280..1491)
                     /gene="thiI"
                     /locus_tag="IW819_002623"
     CDS             complement(280..1491)
                     /gene="thiI"
                     /locus_tag="IW819_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1245705.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            complement(1507..2649)
                     /locus_tag="IW819_002624"
     CDS             complement(1507..2649)
                     /locus_tag="IW819_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1245706.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
BASE COUNT          766 a          601 c          470 g          972 t
ORIGIN      
        1 taaattcctt cttatttctt gaagctgccc acttctgtca caacctcgct atttcatggg
       61 aaattctctt ttttatatca tttatcaact gtagcttgat ctctaaataa aattttattt
      121 ttcagttagt aaaaaagaac cggcttttcc ataacttctt gataaatggt attcaatcag
      181 ctaatcttcg acattagttc aaaaacggta aaatacatgg agctattttt tcgttttgtt
      241 tctctaatac tctggtcaaa acactatttt cataacctct tatagcaatt ctgaaaattc
      301 ttcagttttt tcattagacg attctgcttg tatctcttcc atcttcaagc cagctagtgc
      361 tcgttggatc atttcatcta ttgctaaacg ctcttcatat tgatggacct tttccagttg
      421 agggcgtgtt tttggctgtg gaggagcaaa aatcgtacag caatcttcaa aaggctggat
      481 agaaagatta aatgtatcga tctgttctgc tacttcaatg atttcaagtt tatccattgt
      541 cgctacaggc cgaatgattg gcgtattcgt tacttcattg atagccacca tactttgcaa
      601 agtttgtgaa gccacttgac ctaatgattc accattcaaa atgactaaac catgacgaat
      661 acggcgaatt tcatcggtca aacgcaacat cattcgacga gtaattgtca tccaatatcc
      721 ttgtggcact gactttttga tttcttcttg aatctcggta aatggcactt cgataaaatg
      781 gatcgttccc acataaggtg ctagtttggc tgttagatct tttgcttttt gcagtgcttg
      841 ttcacttgta tacggcggac tagcaaaatg aacagcttca atttccactc cccgcttcat
      901 ggcaaagtac cctgctacag gagaatcaat ccccccagaa agcataagca ttccttttcc
      961 gcttgttcct acgggcaatc ctccagctcc cttgatcgtt tcatacgata gataagcgcc
     1021 atcacgccga atctctacac gtaatggaat atctggttga cgcatttgaa cttggatatg
     1081 cgggaaaata tcaaaaacag cattacctaa tgtttgattc aattcattac ttgtcaattc
     1141 aaattggtga tcgcttcttt tagcgataat tttaaatgtt tctttcccag tataaatttc
     1201 tttcatgatg tcttgaacag cttgtttcaa aacttcgaca tctttttcta cacgaatact
     1261 tggagagaaa ttttggatcc caaatatttt tgctagtttt ggaataacta gctgcccgtc
     1321 tgcgccattc aataaaagat gcatccggtc ccgatcagca tggattttga tttctggaaa
     1381 gtctttcaaa gcttgtctga cattttgtgc tagttgcatg ataaagcttt ttcgattctt
     1441 tccttttgtc gaaagttccc cataacggac cataatttca ctgtatttca caagaacact
     1501 cctcttttaa ttgattgcag aaaatttctt ataaagctgc ttgaaaataa tcataaattg
     1561 ctcgacttct gcaagcgaat tgttttcatc taagctgatt cgaacagcag atgtagccag
     1621 atcatttggt acatgcattg cgtgcaatgt actgcttgcc atatgtttac gtgaggagca
     1681 ggcactagta gtagaaacaa atatttgttt ttcttctaag gcatggacga gaacttcgcc
     1741 gcgaattcct tttagagcaa aacataaaat atgcggtgca tagtcagttc cttctgaaaa
     1801 aatcgtcact ttctcatacg tcttcaacgc attgattaga gctactttta agcgttgtgt
     1861 ctgctcaggt ttttgttcct ttatctctaa tgataaacgt aaagctttaa ccatagccac
     1921 aatacctgga acattttctg tcccacttcg ctgatttctt tcttgccctc cacctgtcag
     1981 taatggtgct aaacgacgac cctttttcca ataaatgaaa ccagttcccc gtgggccatg
     2041 gaatttatga gctgaaaatg ttgcaaaatc aactctttct gtcaaccatt gcgagaatgg
     2101 tacttttcca atcgcctgaa cagcatctac atggaaatga attttaggaa aatcatccaa
     2161 aagatcactg atttcctgga tgggctgaat cgatcctact tcattattca cagccataac
     2221 agagactaat actgtctctt ttcttattaa ttctttcaat tcgtcgacac gtacaaatcc
     2281 gcgtttatcc actggggcga tacttacttc gattcctaat tcttttaatt gttctgctgt
     2341 ctcgctgacg gccggatgtt cgactcctga aatgatcata tgattcccat attctctttt
     2401 ttcgatcatc gttcctttta atacccagtt atctccttct gttccgcctg aagtaaaata
     2461 gatttcttct ggttctacat ggatcaaatc agcgatctgc tttcttgctt gctgcaacaa
     2521 ccggttagct tgattgccta aatcatgcaa gcttgatgga ttcccaataa tccgctgact
     2581 ggtttttaca tatgcatcta agctttgcgg aaagattggg gttgttgcac tgttatcaaa
     2641 atatatcata agaaacccct cttttttgaa attcttttct attataacgg ttgtccaagg
     2701 tgctttcaag agcaggtgaa attttcactt gtttcaattt tcaaatatac aaaaaaagac
     2761 aagaacaaga caataatccg tcttgctctt gctggtttac taacttttt
//
LOCUS       DACEPW010000253         2798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.259, whole genome shotgun sequence.
ACCESSION   DACEPW010000253 DACEPW010000000
VERSION     DACEPW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.259"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW819_002625"
     CDS             complement(543..2048)
                     /locus_tag="IW819_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1245707.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW819_002626"
     CDS             complement(2045..2731)
                     /locus_tag="IW819_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1245708.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
BASE COUNT          806 a          557 c          429 g         1006 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt tatctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgctt
//
LOCUS       DACEPW010000254         2793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.261, whole genome shotgun sequence.
ACCESSION   DACEPW010000254 DACEPW010000000
VERSION     DACEPW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.261"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            45..929
                     /gene="rapZ"
                     /locus_tag="IW819_002627"
     CDS             45..929
                     /gene="rapZ"
                     /locus_tag="IW819_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1245709.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            926..1924
                     /locus_tag="IW819_002628"
     CDS             926..1924
                     /locus_tag="IW819_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1245710.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            1958..>2793
                     /gene="whiA"
                     /locus_tag="IW819_002629"
     CDS             1958..>2793
                     /gene="whiA"
                     /locus_tag="IW819_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1245711.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELG"
BASE COUNT          902 a          498 c          632 g          761 t
ORIGIN      
        1 atgatataat tttattatgc acaatgtgag aggggttcgt aaatatgaca gataatttgc
       61 aattagtcat cataacaggt atgagtggtg cagggaaaac agtagctatc caaagttttg
      121 aagatatggg ctatttttgt atcgataata tgccgcctcg attgattcca aaattctggg
      181 aattgatcaa agaatcaggt aaagtcacaa aaatggcttt aggagtggat ttgcgttctc
      241 gttccttctt tgaagaaatc caaaacatgc tgatcgaaat cgaaaatacg aacttcatcg
      301 acacttctat cttattttta gatgcttcag acgaagaact agtggctcgc tacaaagaaa
      361 caagacgagc acatccaatg gcgatggacg gtttgatcac tgaagggata cgtaaagaac
      421 gggcgatact agacgacttg aaagcccaag cttccgtgat cattgatacg acaacattga
      481 caccacgaca attacgtgaa aaaatcaatg agtcattcaa atcaaaggga gaccaaggat
      541 ttcggatcga atttgtatca tttggtttta aatacgggtt gccgatcgat tcggatattg
      601 tgatggatgt gcgttttttg cccaaccctc attatatccc ggaactaaga ccattgaccg
      661 gaaaagatca gccagtctat gattatgtga tgtcctttga tgaaacgcaa tcattctacc
      721 ataaattttt ggatcttctg gaaactgtat tgcctggata cattaaagaa ggaaaaagca
      781 gcttgacagt tgcaatcggc tgtacaggcg gtcaacaccg ttctgtggca ttgactgaac
      841 gtttggcaaa tgctttaggg aaaaactata aagtcaatgt gacacatcgg gacaaggata
      901 aaaggaaaga gacggtgaac cggtcgtgaa aatgaaaacc taccggataa gaaaaccaaa
      961 gatcgtagtg gtcggcggtg gcactggtct gccagttatc ctgaaaagtt tacgaaatca
     1021 aggagcagat atcacagcag tagtgactgt tgcagatgat ggtggaagca gcggtgcaat
     1081 cagagaatcg atcgctatgg ctcctcctgg tgacttgaga aatgtcttag tggcgttatc
     1141 ggatatgcca caattctatg aagatatttt ccaataccgt ttcaaaaagg aagatcagtt
     1201 tttagccaat catacgatcg gtaatctgat cattgccgca gtttccgaga tgcggggaag
     1261 cacttatgag gcgattcaat tattagcaaa aatgatgcat gtagatggac atgtttatcc
     1321 ttcttcagaa actcctttga ccttgcatgc tgtttttaaa gacggaacga ctgcagtagg
     1381 agaatctaaa atcgcgatca accgtaaaac cattgaccgt gtgtatgtct gcaacacaaa
     1441 tgataaaagt gaagccaaag cggctcgaaa agtcgtttca gcaattatgg acgccgacat
     1501 ggtcgttttg ggacccggaa gtttattcac cagtatcctt ccaaatttag tgataccaga
     1561 aatcggcgaa gcaatgctca agacaactgc tgaaaccgtc tatatctgta atatcatgac
     1621 ccaaaaaggg gaaacggaac atttcacaga tgccgatcat atacgtgttt tacacaagca
     1681 tttgaatcag ccttttatcg atacagtatt ggtcaatacg gaaaaagtgc ctgaagatta
     1741 tatggaccca gagatctatg atgagtatct agtacaagtc gcacacgact tcaacggatt
     1801 gagaaatgaa ggatgtcgag tgatttccac cgacttttta gaattgaaag atggtggtgt
     1861 cttccacgat ggagacaaag tagtagaaga attgttccgt ttagttttcg gttcaaaata
     1921 ttaagcaatt tattgttgga gaaaggagga gaataggatg tcatttgctg cagatgtgaa
     1981 gaaagaacta actggtttgg aagttcatcg agaacatgcg aaagcggaat tagctgcttt
     2041 gctgcgaatg aacggctctt taggtattgt gaaccagcag tttattttga acgtccaaac
     2101 agaaaatgct gcaattgcca gacgtatcta ttctcttttg aaagatcatt atcaggtacg
     2161 aagtgagcta ctcgttcgta aaaaaatgaa attaaaaaag aacaatgtct atatcgtccg
     2221 gttgaagcaa ggcacaaaag atgtgttgat ggatcttgat atcatggatg gtatgatgtt
     2281 caatggacac gtttcggacg agattatggg aaattcgcaa aaaatgcgtt cttacttacg
     2341 tggtgctttt atggcctcag gttcagtcaa taatccggaa acgagtcgtt atcatttgga
     2401 aatattttct atttatgaac agcataacca agacatttgc gatatgttaa attattacgg
     2461 tctacatgct cgaacgcttg aacggcgcaa tggctatatt tcgtatttaa aaggagcaga
     2521 aaaaattgct gatttcttaa cattgattgg tgcaaccaac tcaatgctcc gatttgaaga
     2581 tgttcgaatc gtacgggata tgagaaattc ggtcaatcgt ctagtcaatt gtgagacagc
     2641 aaatctgaac aagacgattg atgcggcttc taaacaaatc gaaaacatcg aattgatcga
     2701 agcaaaggtc ggtctacatg cccttcctga aaaacttcag gaaatcgcag aacttcggct
     2761 tcaacatcca gaggtcagct tgaaagaatt agg
//
LOCUS       DACEPW010000255         2760 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.262, whole genome shotgun sequence.
ACCESSION   DACEPW010000255 DACEPW010000000
VERSION     DACEPW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.262"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1565)
                     /locus_tag="IW819_002630"
     CDS             complement(<1..1565)
                     /locus_tag="IW819_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245712.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHV"
     gene            complement(2237..2505)
                     /locus_tag="IW819_002631"
                     /pseudo
     CDS             complement(2237..2505)
                     /locus_tag="IW819_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          768 a          599 c          442 g          951 t
ORIGIN      
        1 acatgcagag tatacgttag ttcattttca ttgagattga taacattttt gtccacactt
       61 tttcttaaag agattgccgg aagattactt tcagggattc tcaagcggta gtctcggctc
      121 attacctgat tattgtaggt catactcgct cgattccgag aaaacccatc ctcttgtctt
      181 ccttcattga acaagtcgac atcggtcact tcaagagcat attcaataaa atacgattga
      241 ccctctgcta gattaccaaa gtccacaaca agtgtattcg tttccttatt aaaagatact
      301 ttgtccacaa attcctcgga aacataatga gttctaacgt ttgcttcacc tggattcgtt
      361 acttctgcga tccgaacccc acctgaaata ggttctctat ttccagtgga actccatgta
      421 gtcggattcg gtgccaaagt tgttccttgc ggtagtacat cggtaaccac tacaccgtct
      481 tttcgtgatc cttcataatt tacctgcaaa ataaatttat tactgttggt cacgtcttgc
      541 ccgtcaatca gcagattccc aagatttcct tggctgtcga tttctctaga gccatagtat
      601 aatttcgaaa aaatgggtct tgatgaagga ataggcgtaa ttgaaaattc cgtgtttgct
      661 gctcgctcag cataagtaag actcacttga aagtcattat ttggctcagt accgcctgat
      721 tgaaagaaag caacttcaaa cgtgatactg aatgtatacg actgagcggc ttcatttaat
      781 ccttcataat caaaggtaaa tggaaaaaca acgtctgtgc cttcttggta aaatgccagt
      841 ggctttaaag gtgaatcatc agcgacctcc actctaatga actcaattaa gccaatcgat
      901 gctctaggaa ctcgtacgac catctcccca tattcttcaa gaatatcgcc agcgcctcct
      961 tgaatgtatc cctttaacgt ttgtgtatgc ctgctcggca aagtggggcg ctcagcagag
     1021 atgccgatgt cataattcaa gagctcctct tgtctttcaa tctgagattc ctgcgttata
     1081 gatggctcag atgatctggc ttcattaaaa gattcactta agctttcttc tggtatatta
     1141 aaagattcac ttgtgctttc ttctggtaca ttaaaagact cacttgtgct ttcttctggt
     1201 acactgattt ctattgagac cgtatttgat tgaatatcac cgcttcttgc ggtcaattcg
     1261 tatttacctg aatgctggga agaataagta atcagacgtt cgtaatcatt gagttgcttc
     1321 acttcccctt caatcaaatc ctcactctta tcaatgacca atgcatttag caggtctggt
     1381 gttcctctga ctcgcaactg gaactgctca cccaaagcag cctcctcttg atcaacttcg
     1441 atcgtcagcg aacgctctgg ctgttctgct aaagactcag ctagaaccgt tatgttccca
     1501 aattgattga tcacaataag cataactaga aacatactta acagtttatg aaatttgttc
     1561 ctcactatta ctccccctta taattttgac taaacaacaa ggtacccgat aattttctaa
     1621 aaagattccc caactcaaaa tggctgcgtt acctccctcg tattttcggt tcaactcaag
     1681 cgttgcaatg taagctgtaa acaaagctaa tgattgccga ttgtcttctg ttgattgatt
     1741 cgatagcttt gtatgacttt tagttgtccg tgtggcttaa ccataattat gatttcacga
     1801 ttcctcctgc cagtgccttg cacaagctgg agcgtaaccc taatagttat tttgttaatg
     1861 tacttatata cttttatact tttccattag aaacaatcac taaatcgttg ataccaaggt
     1921 cagactctag aatttcatca gtgagtccct gtattggtta accttcatca atcccaaaaa
     1981 aatatatcaa tgcttagcat caaaatgaat atatagttta gaatacttgg gattcatcga
     2041 taactgatcg cttatttggc catattattt ggcacttggt tcaactaaac gcatttagga
     2101 tgttccttca ttctcgtcat ggatgttctc aaaccgtcat tactaataaa ctgtccaccg
     2161 aatcgatggt tcatttcttg cattttccac gtccaattac tctttttgct ctttatttgg
     2221 gcgtagtttt actttcttaa cttgtatcgt cttccatcaa ctccttaatc atcttcttag
     2281 ctttattggc tcggtttcct tgtaaaaggt atctacaaat agtgacaatc tgtattaaat
     2341 cttcatcatt ttctgtaatg tcaataattt ctatattctg taccatatat tgcaaagggt
     2401 ttcactaagt tcataaccga agcgaattaa acaacttttg tatagaataa ctaccttttc
     2461 tatttttcca ttggtaattc tatctattaa ttggtttaat ccttttgttg tagttgattc
     2521 cactaccaag atgattctct cgaattttaa agcttgaaga tgtattgcta tttttgctaa
     2581 ctggcgtatt tgcaactatt tttcccttct attcttcctt cattgattcc cctctaaaat
     2641 tactatccat aaaaaattgc ataaatgaca atagtattta tattagtcat actgcttttt
     2701 aacacagcaa attccagaaa aacattcgtt acatatttgt gaatttttcc aaaaaataac
//
LOCUS       DACEPW010000256         2745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.263, whole genome shotgun sequence.
ACCESSION   DACEPW010000256 DACEPW010000000
VERSION     DACEPW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.263"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..554
                     /locus_tag="IW819_002632"
     CDS             <1..554
                     /locus_tag="IW819_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1245713.1"
                     /translation="LTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGPFLWYS
                     LLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASDPNNIV
                     IRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKSNVLVY
                     SEGGLWSSLKQSISIMRNERKKA"
     gene            715..2613
                     /locus_tag="IW819_002633"
     CDS             715..2613
                     /locus_tag="IW819_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1245714.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
BASE COUNT          921 a          472 c          536 g          816 t
ORIGIN      
        1 cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt caattcaaag
       61 atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg attggaccat
      121 tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct gttttagcag
      181 ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt ttgccagtga
      241 cttatctatc tctaggcgga tacatgcttg gattgatact aggagcttca gatccaaaca
      301 atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt atgccgattc
      361 gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg gtacttgtag
      421 ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat gtactcgttt
      481 atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg aggaatgaac
      541 gaaaaaaagc ataaaacaat cctaaaatac agaaggaagc tggacatcta ttgttcccag
      601 cttctttttg tatagaccta ttttatttat tttactatat tggatatata ttcattgaaa
      661 atagtgtata acaactttat aatattgagt ataccaattt ttggaggcgt tttaatggac
      721 cgttatttag atttactttc agaaaaattc ccctcaacgg aggatgtcat cacggaaatc
      781 atcaatctcg aagcaatttt gcagcttcca aaaggaacag agttattttt gagtgatata
      841 catggcgaat ttcctgcttt tgatcatatt ctgcgtatcg gttcaggaaa tcttaaagaa
      901 aagatcaaag accttttttc tgaacaaatg acggaaacgg atatcagcca attcactatt
      961 tttacggctt atccagaata tgcattaacg actgattggt acaaagaaca agataaatcg
     1021 cagctgatcc atcatttgat cgatctttta cggtttacga cagtcaaata tacccggtca
     1081 aaagttcgaa aaggattgcc gaaagaatat agctatatca ttgaggaact cttgtatatc
     1141 gatgaccgta tcaatggaaa aaaagactat gtaaaaaaaa tcattgaaca acttgttttg
     1201 atgaaggaag aagaaaaatt tttaacgaaa cttgcacaga cgatccaaaa atctgtcatc
     1261 gatcatttgc atatcgttgg agatattttt gaccgaggaa ctcaagcagc caaagtgatg
     1321 gatcgaatca tggatttttc ttctgtagat atccaatggg gaaatcatga tattttgtgg
     1381 attggtgcgt attgcggctc tgaggcatgt ttactgacac ttttacggat tgcagcacgc
     1441 tataattatt tatttgaatt agagaaagaa tatgggttga atttacgtcc attgttttct
     1501 tttgctcatc aaacttatca gaagaatcca gtatttgcgc ctaaaaacag taaaaatcta
     1561 tccgaaagag agcaagaaga attagaaaaa atccatcagg cactttctat ccttcaattc
     1621 aaatcagaac atcagttatt ggatcgtcga cctgaattta aaatggacga tcgtaaattg
     1681 ctggaaaaaa tcgactatga agcatcaacg attgatttag aaggtcagct ctatgagttg
     1741 aaaggaactt gttttcaaac gatccagcca gatgatccaa aaaaattctt ggaagaggaa
     1801 caaaaagtga ttgatagttt gatgtattca ttccagcact ctttgagaat gcaaaaacat
     1861 atgacgtttt tgatcgaaaa agggggaatg tatctcacga acaatcagca tgtgctgttt
     1921 catggctgta tacctgtaga cgagaaggga cagctcttag catttgaatt agaccaatct
     1981 tatcgcggaa aagaactgct gggctttttt gaaaaacaga taactgaaag cgcaaaagat
     2041 ctgactgcaa aagaagattt agcaaccgat ttgatttggt atgcgtggag tgggccattt
     2101 tcgcctttat tcggcaagag taaaatggcg acttttgaac gttattttct cacagagcct
     2161 catacgcacg tcgagaaaag caatccttat tatcacttgc gagatgaaga gtggttcagt
     2221 gaaaaaattt tagcagaatt cgacacaaaa gaagaagggt cagcaatcat taatgggcac
     2281 acgccagtaa aagtaaaaaa aggggagtca ccgatcaaag caaatggaaa agtatttgtc
     2341 attgacggag gtttatccaa agcttatcaa aaaacgacag gaattgccgg ttattcacta
     2401 ttatgcaatt cttatggttt ccaaatcgtg acacattatc cgtttttgcc tattgaacaa
     2461 caatttgcaa aaaaatccga ccaaacaaat gtgaaaaaag taatcgaaca gcaactacca
     2521 cgcaaactca atcgtgatac aacaaaaggt atggaactgc agcagcagat tacccagctc
     2581 aaaaggctgt tggactatcg aaaggacgat tgagaagaat tccagtgaac attgttccat
     2641 agactggaga ctgagacaaa agtcgtgtcg cagtctcttt ttccggataa acggtgttct
     2701 tctgccaact cctcggtcac aagtcacact attcaacaaa catga
//
LOCUS       DACEPW010000257         2725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.264, whole genome shotgun sequence.
ACCESSION   DACEPW010000257 DACEPW010000000
VERSION     DACEPW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.264"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(175..645)
                     /locus_tag="IW819_002634"
     CDS             complement(175..645)
                     /locus_tag="IW819_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245715.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            732..1430
                     /locus_tag="IW819_002635"
     CDS             732..1430
                     /locus_tag="IW819_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1245716.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1608..1949
                     /locus_tag="IW819_002636"
     CDS             1608..1949
                     /locus_tag="IW819_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245717.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1942..2613
                     /locus_tag="IW819_002637"
     CDS             1942..2613
                     /locus_tag="IW819_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1245718.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
BASE COUNT         1000 a          397 c          555 g          773 t
ORIGIN      
        1 tacgctttag gactttgggg aagcaggcaa gcgttgatag gaacgattgt ttacatggtt
       61 tggattttta tggggcttga tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag
      121 ggggctgact aaaaatgaat agaaaagaga aactagaatg tatattacta ttactcagtt
      181 taattctttc actaatttct ctattgggaa gtttgtattt ttgatatcaa caattttgtg
      241 gatttgggtt ctatttagag taaatatcat ttttgtttgg tgattaagga gtttattaaa
      301 attaattttc cctacttcaa atgcaaggta acaagatata gcacttttag gaggaatatt
      361 tagcggtaca gcttcagagt atattctatt atcatcgact gatcctcctt tagcaattaa
      421 tttcttaaat ggagtagctg accatctgcg gtttaagtta gcggatgaaa gttctaaatt
      481 aactaacata gctggttctg ttgaatagtt agaaatgatt acttttgtat aaatcatatc
      541 tgcagcgaag taagaagcat taagttcaac ttctagttga gggcgcattc cttttatttt
      601 tatccctgtt aaaaacgtac tcaatataaa acttattatt gacatccatt gaaaaattgt
      661 taagtgaaaa aaattcaaaa taaacaccac cagtttttaa ctaaattata ccaaaaagga
      721 gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa agaagctgtg actacgagct
      781 tacaagtggc agaaagcttc gagaaaaaac atcaacatgt tttaagagat attgatgcac
      841 taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa ccagattcat
      901 atggcagaaa tcgacgagtt ttcttcatta gtagagatgg atttttcttg ctggctatgg
      961 ggtttacagg aaagaaagct atctacttca aacaaaaata cattgaagca ttcaacgaaa
     1021 tggaagatgt tattcgcaag aatactgttc ctcaaacaat tgaagatatg atgatctatc
     1081 aattagaaga aatgaaagat gttaaaaaag atgtttccat gcttaaagat actatgcgaa
     1141 ttagcggaca acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt atgggagttc
     1201 tagggggaaa agaaagccga gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt
     1261 ggtctgaatt taaacgtacc ttttcaatcc caagatatgg cgagttacct cgtaagagat
     1321 tcgatgatgc tgtttcattt attgaaatgt ggttgccaga aactgcgatc cgtatggaaa
     1381 ttgatcaact gaacagacaa cagagacttt ttggtgatga caatgaatag agctgaagcg
     1441 ctaagaatag ggacggcaat tgctaattgc tggtggaaat actataaacc aatcatccta
     1501 agccaacaac atattgacaa gcaaaaagca tggcaacaaa taaaaaagtg actccgccgg
     1561 caagcaaaga gtcacaaaga aaacacatca taaggagatt ttagcatatg gaaaaagaac
     1621 tttccactct agatcaatat ttgattgatc ctgattgggg caagccgaaa attgaggaaa
     1681 caagtggtcg aaaaatcaga cgaaatcttt tgacgaatga agaactagct tgggatcaag
     1741 atgatttagg caaccatgta actatttggg atcatgttta tcttatccat ctatcgaagc
     1801 attcgaataa acctgaatat atttacgtca tcgaagatgg cttgattgat gcgctagaag
     1861 agtacgacag agataacttg attgatatct cttattacgg accaggtaag aaatacattg
     1921 ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga caacgaactt tgaaaaactt
     1981 tttagtcgta agttaaataa aattctcaag aaaaaaggaa attttgatta tttatcttgg
     2041 gctcacgcgt gggagattat gaaaaagaat gatccacagg caacggtaac tattaatgag
     2101 tacaaacact acagggttgt ttctggaact catcaagact ttcttgttga ggaatataaa
     2161 ccttttctta tggatgaaac tgggacttat gtatctgtct cagtaacggt taaaggacac
     2221 acggaaaccg aattatttcc tgttttagat tatcgaaacc aaccagttgt taaaccaaat
     2281 gcaatgcaaa tcaataactc attgaagcga tgctttgtga aagcattggc tctacacgga
     2341 ctgggattat atgtatttca aggggaagat attccaacac cacctagaat cgatacaaag
     2401 aaattaaaca tgctagagac gattctagaa gctttcaatg agcagatggg taaagatatg
     2461 accaaaacct taatcgaata tgttaatgag cagacagata aattagggct cttagctgat
     2521 aacgttgaaa ctattgaaca gttaagctat gagcaatgtg ccttgatgga gcgagcaata
     2581 gcagctaaga gaaaagaatt agataagaag tgatatgagt gtttaaacca ttaatcgatt
     2641 catattcagc ggttctgaaa aagttcaaag gaaaagacat aagcgcaacc atcaatgagg
     2701 aagtgaacat tgatcgacta aagac
//
LOCUS       DACEPW010000258         2706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.265, whole genome shotgun sequence.
ACCESSION   DACEPW010000258 DACEPW010000000
VERSION     DACEPW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.265"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            170..514
                     /locus_tag="IW819_002638"
     CDS             170..514
                     /locus_tag="IW819_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1245719.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IW819_002639"
                     /pseudo
     CDS             515..934
                     /locus_tag="IW819_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="IW819_002640"
     CDS             complement(1026..1376)
                     /locus_tag="IW819_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245720.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IW819_002641"
     CDS             complement(1342..2673)
                     /locus_tag="IW819_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1245721.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1002 a          490 c          522 g          692 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actata
//
LOCUS       DACEPW010000259         2692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.266, whole genome shotgun sequence.
ACCESSION   DACEPW010000259 DACEPW010000000
VERSION     DACEPW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.266"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(57..1433)
                     /locus_tag="IW819_002642"
     CDS             complement(57..1433)
                     /locus_tag="IW819_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1245722.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1561..2679)
                     /locus_tag="IW819_002643"
     CDS             complement(1561..2679)
                     /locus_tag="IW819_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1245723.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT          845 a          553 c          440 g          854 t
ORIGIN      
        1 aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat
       61 cttcttggct cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc
      121 gcttctgttt cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc
      181 atttcttcag aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc
      241 ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc
      301 agatcactgt caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat
      361 tcgtgttcat cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct
      421 ccgacgattt cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca
      481 tctaataaaa tagccatctg attctgcgtc tttttcattt catataacag atcatcaata
      541 aagaccgttt caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca
      601 aagccgaatt tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta
      661 tctttgtctt cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca
      721 ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact
      781 tctcgggcaa ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta
      841 ttcaatacgc cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct
      901 tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc
      961 cagacgaagg gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg
     1021 acttcttctg atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat
     1081 acataggtca aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca
     1141 cctaataccg gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg
     1201 ttgaccaaag tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt
     1261 tttaaaagct tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta
     1321 ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg
     1381 atcaataata aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca
     1441 ttctctcctt ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca
     1501 cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac
     1561 ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt
     1621 gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc
     1681 aagcaatccg gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat
     1741 tttcagtgat ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat
     1801 tagcacaacc acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa
     1861 tgcaggtgct agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat
     1921 gaccccaaat aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt
     1981 accaacgaat aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag
     2041 ttgccccaat aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt
     2101 tggcactagt ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat
     2161 tactacggtt gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact
     2221 aaggaattgc tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat
     2281 atctacttcc ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt
     2341 gatgaaatca ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac
     2401 actaagtaaa atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca
     2461 tattcttttg atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag
     2521 aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa
     2581 aataaagttt atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga
     2641 ccaaaacatt agtttagact gtctaagttt ttcaaacaat agatttcctc ct
//
LOCUS       DACEPW010000260         2689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.267, whole genome shotgun sequence.
ACCESSION   DACEPW010000260 DACEPW010000000
VERSION     DACEPW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.267"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..837
                     /locus_tag="IW819_002644"
     CDS             <1..837
                     /locus_tag="IW819_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1245724.1"
                     /translation="MVINKKAIAPLIGTSLLVGPMMVTAVPQHVHADEQGSQTVADPQ
                     AFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLASAPNYNLFGVKGSYNGQV
                     VYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTSFSTGDYHYAGVWKSNTT
                     SFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTGSATTITSTTTTTTTTTS
                     TSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMAKNGMSMSSYSLLVGQEL
                     NV"
     gene            977..1903
                     /gene="cysK"
                     /locus_tag="IW819_002645"
     CDS             977..1903
                     /gene="cysK"
                     /locus_tag="IW819_002645"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1245725.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2138..2584
                     /locus_tag="IW819_002646"
     CDS             2138..2584
                     /locus_tag="IW819_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1245726.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          895 a          438 c          586 g          770 t
ORIGIN      
        1 atggtgatca ataaaaaagc gattgctcct ttaattggaa cgagtctttt agttgggccg
       61 atgatggtta cagcggtacc gcaacatgtt catgcagatg aacaaggatc tcaaacagtc
      121 gctgatcctc aagcgtttat cgatcaaatc ggctggtctg cgtcagaagt tgctgcaagt
      181 aatgacttgt atgcttcagt catgatcgct caagcgttgt tggaaagtgc atatggcact
      241 tccggattag ctagtgcgcc aaattataat ctattcggtg tgaaaggaag ctataacgga
      301 caggtcgttt atatggcaac caaagaatat gtaaacggac aatgggtaac gatgaatgag
      361 ccattccgaa aatattcttc ttattgggaa tccttccaag accatgcgaa tgttcttcgt
      421 tcaaccagtt tttccacggg cgattatcat tatgctggcg tgtggaaaag caatacaaca
      481 agtttttatg atgcaacagc gtacttgaca ggtcgttatg ctaccgatcc aaattatggg
      541 gccaaattga actcattgat taatacttat aatttaaccc gttttgatac gccatcaaca
      601 ggttcggcta caacaatcac aagtacaaca acaacgacta ctacgacgac ttcaacgtca
      661 tcatcttcaa caacagatgt aaatacaaat aatggtcaga catatacagt cgtgtcaggt
      721 gatacgctat gggatattgc acaaaaattt ggcatgtctg ttgatgatct aatggcgaaa
      781 aatggcatgt ctatgtccag ttattcttta ttagttggtc aagaactaaa cgtttgattt
      841 agataaaaag gcaaagaaat taccatatat ggtatatctt tgtctttttt ttgctagtca
      901 aagacgaatc actcgccata aatgaacaga ttgtattatg ataagtaaga agagcaaaga
      961 caaggaggaa ttgaagatga cagctatcta taactcagtc accgaattga ttggcaaaac
     1021 gccaatcgtc aaattaaata aaatcgtacc agaagattca gcagatgttt ttgtaaagct
     1081 tgagtttttt aatccaggag gaagtgtaaa agatcgtatt gctttaagta tgatagaaaa
     1141 agcagaacac gatggtctac tgaaaccagg tgatactatc attgaaccta cttctggaaa
     1201 tacaggaatc ggattgtcga tggtaggagt tgcaaaagga tataaagtaa tcatcgtgat
     1261 gcctgagacg atgagtatcg aaagacgttt attgatgaaa gggtacggcg cagagttgat
     1321 tttgactcct ggtgcggatg gtatttctgg atcaatcaga gaagcagaac gtttggcaaa
     1381 agaaaacggc tactttttac cattacaatt tgaaaatgaa gcaaatcctc ttgtgcatga
     1441 gaagacgact ggtcctgaga tacatcaagc atttggagta aatggcttgg acgcgtttgt
     1501 tgcaggtatc ggtacaggag gaacgatcac tggcgctggc cgagaattaa aacgtgtgta
     1561 tccgaaaatc gaattaatcg gggtagaacc agcagaatct gctattttag aagggaaaga
     1621 agcgggacct cacaaaatcc aaggaatcgg tacaggtttt gttcctaaaa cactggatac
     1681 ttctgtgtat gataaagtcc tttctattag tggagacgaa gcgatggaaa cagctagaga
     1741 agttgggcga aaagaaggga ttttagtagg catctcatct ggcgctgcaa ttgctgccgc
     1801 gctaaaagtc gcaaaagaat taggaaaagg caagaaggta ttagctgtgg ttcccgacaa
     1861 tggtgaacgt tatttatcta cagcccttta ccaagagata taaggcactt ggtttttctc
     1921 cagatagttt atgtaaaaaa cttttaaaga tgaaaagaac acacaaaaat ttatgatgag
     1981 aaaaattatt tgaatagaga cgtagaatag tgaacgcgac tgttttacgt cttttttcat
     2041 taaaatacaa taaaattgaa ttttcttttc atgttaagtg gattttaaaa tgtaaaaata
     2101 gtatgataag acgagaataa caacgtgggg tgtttacatg gaaaataagt tagtcgaaga
     2161 ggttatcgaa aagttaaaaa aagagaacat tcgaatcaca cctcaacggt acgcggtttt
     2221 agaatatttg attgaacatc attcccatcc aacggctgat gagatttata aagcacttga
     2281 acatcggttt ccaaatatga gtgttgcgac tgtttacaat aaccttcgct tatttacttc
     2341 gattggattt gttcaagaaa tgaagtacgg cgatgcttct agccgatttg atttcagttc
     2401 aaagcgtcat tatcatgtaa tttgtgaaaa ctgcggtaag attgttgatt ttcattaccc
     2461 agggcttgaa gatatagaaa tggctgcaag cagactgact gattttgaga ttaatgaaca
     2521 tcggttggaa ttgtatggac tctgtccgga ttgtaaagag tcagcaaaaa aggcgcaaat
     2581 gtaaaaacag tttctacaag tgttatacct aaaaaaagct agctgtgtaa aaaaaacgct
     2641 agctttttta tttttttgtc ttttattcac aatcttagat ataaaagtg
//
LOCUS       DACEPW010000261         2682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.268, whole genome shotgun sequence.
ACCESSION   DACEPW010000261 DACEPW010000000
VERSION     DACEPW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.268"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            325..1503
                     /locus_tag="IW819_002647"
                     /pseudo
     CDS             325..1503
                     /locus_tag="IW819_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            complement(1620..1763)
                     /locus_tag="IW819_002648"
                     /pseudo
     CDS             complement(1620..1763)
                     /locus_tag="IW819_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            1845..2621
                     /locus_tag="IW819_002649"
     CDS             1845..2621
                     /locus_tag="IW819_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1245727.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT          933 a          447 c          470 g          832 t
ORIGIN      
        1 cggtagtagg aatacttaat acctcagctt ctgaggaggt atcccaatta tagttatact
       61 ttgcatcatc accataaaga acaattccat tttttttatc ttgtgccgtt agctttactg
      121 gttcgcttac ttttcttgtt aatatcttgg actttacatc atcgatattg cttattttat
      181 caagcttaaa taatttacaa attaatttcg tactagtttt tatatttgac gagagtgtaa
      241 aatattttgt gtaaatagaa aaaaggaagt cccttctgta gaatagagtt accacaacac
      301 attcacagaa aagaggactt ccatatgaac gattttacta cagaaattct aaagactcta
      361 gcgaacaaag gcgatttgaa tgaattattc cgtgtccatt tggaaaaagc tgtcaatacg
      421 cttctcaaaa cggagttaac ggctttcctc gattacgaaa agtacgatcg cattggtttt
      481 aacacgggga attctcgtaa cggctcctat gaccgtacgg tcaagaccga gtacggggaa
      541 cttcatctcc agattccgcg cgaccgcaac ggcgagttca agcaacagac tgttcctgct
      601 tatagacgga cgaatgatac cttagaggag accgtcattc acctcttccg aaaaggtatt
      661 accatgtcgg aaatcgcaga cttgattgag aaaatgtatg ggcatcacta cacgccccaa
      721 accatgtcca acatgactaa agtgctgact gaagaagtaa atgcatttaa agttagaact
      781 ctaaatgata agtatgtcgc tattttcatg gatgctactt atattccttt aaaacggcaa
      841 accgtctcca aagaagcaat ctatattgcc attggtatac gagaagacgg cactaaagaa
      901 gtactaagtt atgcgattgc tccgactgaa tcgacctacg tttggaatga gttgttacag
      961 gacatttact ccagaggggt tcatgatgtc ttactgttta ttaccgatgg cttaaaaggc
     1021 atgaaagata cgattcacca gatttatcct aaagctaaat accaacattg ctgtgttcac
     1081 gtttcccgta atattgctca taaagtacgt gtcaaagatc gaaaagaaat ctgtgatgat
     1141 tttaaggctg tttatcaagc tagctcaaag gaagaggcaa ataccttttt agggagtatg
     1201 attgagaagt agcaaaaaac ttatcctaaa gtgacgcagt cactgataaa aaatcaagat
     1261 ttattgactt tttatgagtt tccgcctggt atccgccgaa gcatttactc aactaattta
     1321 atcgaatctt tcaagaaaca aatcaagaaa tacagccgca gaaaagagca gtttcaaaat
     1381 gaagaatcac tagagcgttt cctagtatcc atctttgata catacaatca aaaattctta
     1441 aatagaagcc ataaaggctt ccaacaagtg acggatacat tagcttcaat gtttactgag
     1501 taacccatta ttttgcagaa ggacgagtta tttacacaaa attattgacg ctcccaataa
     1561 aaaaacaaac aaagtaaaat aatctacttt gtttgttttt attttaatta atattgaatt
     1621 tataaaattg tcttatcgct tggttccaca accaagtagc gataaggctc atccccttca
     1681 gaatgagtgg taacataagg atttttactt aaaacagaat gaatttgttt tctttcaaaa
     1741 gcgggcattg gttagtcaaa gactagttca tttatattta tatcttaatc ttttatttta
     1801 ataaattgtg atataatgaa gtataacttt ttagggagtg tactatgaat tttcaagata
     1861 atagaccatt atatctacaa gtagaagctt ctatacgtaa cgatattcgt gaaaaaaaat
     1921 atcttccagg agagcaatta cctacagaag aagaattatg taaaatttat ggagtaagta
     1981 agattaccat tcgaaaagct tttaaacttt taacagaaag tggaacagtt gaacgattaa
     2041 gggggaaagg gacctttgta actcaaaaaa aagaaagttt aaaattagct ggagttcatg
     2101 gatttagcga ttctctttct acccgaggac ataaaattag atatactgtt ttacattcta
     2161 aaattataaa ggctgatgag tttttagcta ataaattaca aatcaatgta actgatagag
     2221 tttacaatat taaacgttta atgtgggaag acggtgcccc gatgggaatt gataattttt
     2281 tcgcatcaga agaaaaattt ccaactttat ttgaaaaagg tagccaatat acttcattat
     2341 ataaacttct agaagatgac tatgatgtca aagctacgag ctctattatg gaaataaacg
     2401 gtttatctgc aacacctgag ctttccgact tattgcaatg tatagtagga gatccgctat
     2461 ttattttaga aaaggttggc aaagataaaa ataatcaacc atttcattat tctcaatcca
     2521 cagttcgttg tgatagaatt agttatgtta ttgaagtcac agaaaatatc actttagata
     2581 cggtgcataa aaatgattca gatcctaaag taatagattg atattaaaag aaatcatacc
     2641 caaagcaatc aattttgatt agatttgggc atgattttta tt
//
LOCUS       DACEPW010000262         2666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.269, whole genome shotgun sequence.
ACCESSION   DACEPW010000262 DACEPW010000000
VERSION     DACEPW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.269"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            244..639
                     /locus_tag="IW819_002650"
     CDS             244..639
                     /locus_tag="IW819_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245728.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            964..1581
                     /locus_tag="IW819_002651"
     CDS             964..1581
                     /locus_tag="IW819_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1245729.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1622..1921
                     /locus_tag="IW819_002652"
     CDS             1622..1921
                     /locus_tag="IW819_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245730.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1955..2122
                     /locus_tag="IW819_002653"
     CDS             1955..2122
                     /locus_tag="IW819_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1245731.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(2167..>2666)
                     /locus_tag="IW819_002654"
     CDS             complement(2167..>2666)
                     /locus_tag="IW819_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1245732.1"
                     /translation="SGKDTERPEFRKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAF
                     MKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLL
                     AQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIK
                     KANDL"
BASE COUNT          937 a          389 c          500 g          840 t
ORIGIN      
        1 tcttaggtgt agggataatt tcatttttca ttgataaaat agttaataat tcacttggga
       61 cagattttac ttatcttgat tttattggag atgaggggaa agttgcaaat ggattttctg
      121 aaaaaattat tcaatttcaa gaaaatagaa gtaaacttaa ttaagaatcg tttactattt
      181 aaacagtgtc cttttccttt ttaaatatat acgataaaat aagtatatga attgtaaaag
      241 gacatgatga ttatggaaga aatagaatgg aataatctga ctcaaaataa taaaaataat
      301 tctggatgtg gaattatctt tatgtttgtt atagttttgg gaatagtagt acttttaata
      361 actgtcaaga tagtagatta ctttgcgaat actcctgaag aacaaataca gagaaaactt
      421 cctgagtttg aaagagtacc accttatgat ccaagagata attcatttca agtaaaaaaa
      481 ttaaaagagt ctccaaatga aatgtggttt gaagttacag ttgaatcaag cgacttcaaa
      541 gaaaaaaaga aagaatacaa ttatattgta aaggcaaaag tatcaaaaag atttattttt
      601 aaccctaaat tagaaatata tagtgttgta atagaataat agtgttaaaa aaattgtaaa
      661 agttaaagaa gtcaaattct gatgtgaaaa ataaaatata ccatggaaga cataaaagaa
      721 aaactggata actagtttat ttaatggcag accgttaagg cctgccattt ttatgtattt
      781 tagcggaata aatataatca tgcgggagtg taaacttact gtttcaaaaa gtatactttt
      841 tgacatgcct tgattttctt tgtttatgta tggcataata accacataat acaatttgaa
      901 acgtttctga tttaatctga ttgttttgaa acaattttag tgaaaaaagg gtagataaaa
      961 acgatgagaa aattttttta tatccgggtc agtagttatg atcaatcgat cgaacgacaa
     1021 ctgatttccg cgaaggaatt aggaatccct gaagaatata tatttgtaga aaaagccagc
     1081 ggcaaagatt ttaaacgccc tgaatatcaa ttaatgaaac ggatgtttcg aaaaggagat
     1141 ttactctatc tacaatcatt agatagacta ggcagaaata aacaaatgat tttagaagaa
     1201 tggcgagaat taactaagga aaagcagatt gatatttctg tgttagacat gcctctgtta
     1261 gatactgcta gttacaagca actagatggg ttagagacgc ttatctctga cttaattctg
     1321 caattactca gctatatggc cgaagacgag cggaaacgaa ttaatagccg gcaaagagaa
     1381 ggcgtagaag ctgcgaaaca aaagggtgta aaattcggca gaaagaaaat tgctttacct
     1441 cctgaatttc ctgcgatcta ttctgactgg aaagcaggaa agataactgc agtctatgct
     1501 atggatcaat tgggcatgaa acgaaatacc ttttatcgac gggtgaaaga atatgaacat
     1561 accttgcacc acttttcttg atagatataa cagctattag acgggaaggg agagtttgaa
     1621 aatggactta acgaatcaaa gggtcgtttt tactggaacg ctgcagcaaa tgacacgaac
     1681 ccaagccatc ggtttggttc actctttaaa cggccattgt caatcaagtg ttacttcaaa
     1741 aacaaatttc ctggtttgtg gtcagtattc taagtcgttg tttgatgatt ctctctatac
     1801 aaaaaaggaa caaaccgcgc gggacttgat tgcgttaggt catgagatta ccattttatc
     1861 agaagtcgaa ttttatgcgt taattagtca acagttgaaa gaatggcaac ggtatttata
     1921 atcgttagta aaatttacaa ggaggaaaat ttttatgaag cgaaaatctt atgatcgttg
     1981 gggcattgaa ataactagtt ttaatcgaaa tattgctttt cacgtggagt gtgactgtgg
     2041 ggaattagca aaaagtgttt atcaaaaaaa ttacttttac tgtcctaaat gccatcgccg
     2101 atacggggta catggaacat agaaacgaaa tagtgcggta gaaaagcgaa tgaatgtagt
     2161 atatttctac aaatcattcg ctttctttat tttataaata gttggtcgag agatcccgtt
     2221 ttcttttgca atctctgcaa ctggaatgcc ttgctttaac atgtccacca cggttttaaa
     2281 aactaagcgt ttttgcggat ctgttgagtt aatagaatac tcgattggtc gtcccttata
     2341 tcggccggct ttttgagcca ataatactcc ttgccgctga cgttcacgaa ttttctcacg
     2401 ctcgttttgt gcaatataac ttagcagtga taaaaacata tcgaacatag cggtatccag
     2461 gagttcattt ccagtattaa aatctaaaaa ctgtgcatct aatattttta acgctacttt
     2521 tttctgtttc ataaaagcaa ctgtattttt tatatcttca taatctcttc ctaaacgatc
     2581 tagtgatgta acgatgacgc aatcaccttc acggatataa tttagtaatt ttcgaaactc
     2641 aggacgctca gtatctttac cactgg
//
LOCUS       DACEPW010000263         2665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.270, whole genome shotgun sequence.
ACCESSION   DACEPW010000263 DACEPW010000000
VERSION     DACEPW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.270"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..116)
                     /locus_tag="IW819_002655"
     CDS             complement(<1..116)
                     /locus_tag="IW819_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245733.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPS"
     gene            complement(118..1620)
                     /locus_tag="IW819_002656"
     CDS             complement(118..1620)
                     /locus_tag="IW819_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1245734.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            1879..>2665
                     /locus_tag="IW819_002657"
     CDS             1879..>2665
                     /locus_tag="IW819_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1245735.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHME"
BASE COUNT          802 a          508 c          528 g          827 t
ORIGIN      
        1 gaagggccaa tgatacagac gacatctcct tcgttgatgg atacattgat gtcgtttaat
       61 actgtgttat cgccgtattt tttgactaaa tgctcaacta agattttttc tgccatctta
      121 cttcaccttc ttttctaaaa cttttgctaa ttttgtcaat gctgtgatca aaatcaagta
      181 catgatcgca atgatcaagt aaacatacgt actttgtgtt gtacgtgcaa caatgatctt
      241 accagtttgc agcaattcga ctacaccgat tgcggaaatg atcgttgtgt cttttaatga
      301 gataacgaac tgattgacga atgatgggat catgatgcgt atcgcttgcg gcaaaatgat
      361 tttttgcatt gtccgattat aagaaagacc taaacttctg gaagcttcca tttgtccgac
      421 aggaacggcg ttgatcccgc ctcgaacaat ctctgcgata taagcacttg cgttaagagt
      481 caaggtgata acaccagcaa ggaagtcagg aatcgtaaat ccaacgatac caggcaaccc
      541 aaagaaaata aagaacgcaa gaaccatcat tggaattcct cggatgatat cgacataaat
      601 actagcaatt gtgcgaagag cgcgtattgg tgctacactg aataatccaa aaatcacacc
      661 gattactata gccaaagcaa aggagatcaa agttaggaga attgttctcc aaagtccttt
      721 caacaatgct tgatagttgt tttggataag tccaagaaat gttgattcat cagcagtgga
      781 agactcactg gaagtttctg aaccatccgc tacatattct ttaataatct tatcgtattc
      841 gcctgttctt tttaattctt tcaatccttc attgaacata ttaagaagct caggattttg
      901 tcctttttta acggcaaatg catattttcc gccagattct tttttgatcg gtgtttccaa
      961 ttcttgtcct tgtttgatcg cataaccgat taccggataa tcatccatca ttgcatcgat
     1021 tgcgccaact tttaacccgt cgtacaattg atcagcagca tcaaaatatt tgatagaata
     1081 gccatattca gattcatggt cgcttaaaaa ttcagcactt tcggtaccaa cttttgctcc
     1141 gactgttttt cctttcaaat cttcataaga cttgatcgaa gtatctcctt ttttgacagc
     1201 taattggatc ccgctatcaa agtaagaatc agaaaaatca tagcttttct ttcgttcatc
     1261 tgttacagtc attccggcaa tcattccgtc tgcttgtcct gattccaaag cttgcaaagc
     1321 agcagagaaa ccacggtagt ccattcggat gttaaatcct tgcagtttgg cgatgttttc
     1381 catcaatttt acatcgatgc cttcgtactc gccctttgcg ttttgaaatt caaatggagc
     1441 aaaagcagag tcactggcaa ttacgtatac gtcttttttg ggctctattt tttgcattgt
     1501 ctcgcttgtg cttgactctg ccgttgtagt gttgtcagct tgtgcaacat gaccaaacgc
     1561 aagtgtcaca agtcctgtca ttaagaaaac aaatgagtga aataaatgct ttcttttcat
     1621 aatcaacctc ctaaatttcc aatattttat tattataggc gaaaagtgct gaatcagaca
     1681 agactgtaag ctcgaaaaaa tgaaaaaaat gcgagtttct aacattttat gttataaaac
     1741 ataacatgta taataagata aaaaagtggc gaaagtatgt tcggtgtggt ataatttttt
     1801 agtaaggaat gcctttgctt tccttttctt tttattggtc gtaaaatgaa aaggaatgaa
     1861 tgaaaaggag gacgaagaat gattgagaga gatacctccc gacactttga gcttgtctcg
     1921 aaataccaac cggctggtga tcagcctgaa gcgattaacc aattagtgga tggcgtagtc
     1981 ggcgggaaaa aagcacaaat cttattaggt gcgactggaa cagggaaaac atatacgatt
     2041 tccaatctta ttgagaaagt gaataaacca acactgatta ttgcccataa caaaacattg
     2101 gctggtcagc tgtacgggga attcaaggaa tttttcccta acaatgctgt tgaatatttt
     2161 gtttcttatt atgattatta tcagcctgaa gcttatgtgc cttcaagcga tacgtatatc
     2221 gaaaaagatt caagtgtaaa tgatgagatc gacaaattac gacattcggc tactagttca
     2281 ttattagaaa gaaatgatgt gatcgtcatt gcatccgttt catgtatttt tggattaggc
     2341 tcgccttttg agtaccaaaa acaagtagtt tcgattcgac agggagcaga actagatcgc
     2401 aatcagttga ttcgtgactt agtcagtatc caattcgaaa gaaatgatat agattttcag
     2461 cgcggtcgtt ttcgagtgcg cggtgatgtc gtggagattt ttccggcttc aagagatgaa
     2521 cgtgccttac gtgtggagtt ttttggagat gaggtcgaac gtatccgaga agtcaatgca
     2581 ctgacaggtg aagttttagg agaaacggaa cacgtagcaa tcttcccggc gactcacttt
     2641 gtgacgaatg atgaacatat ggagc
//
LOCUS       DACEPW010000264         2640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.271, whole genome shotgun sequence.
ACCESSION   DACEPW010000264 DACEPW010000000
VERSION     DACEPW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.271"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..911
                     /locus_tag="IW819_002658"
     CDS             <1..911
                     /locus_tag="IW819_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1245736.1"
                     /translation="KTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDL
                     QVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLK
                     QKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFL
                     ANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQ
                     NVAACTSEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLK
                     EDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            1152..1745
                     /locus_tag="IW819_002659"
     CDS             1152..1745
                     /locus_tag="IW819_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ1245737.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            1758..>2640
                     /locus_tag="IW819_002660"
     CDS             1758..>2640
                     /locus_tag="IW819_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1245738.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMN"
BASE COUNT         1058 a          408 c          535 g          639 t
ORIGIN      
        1 taaagacagt cggaaaagta ccagtagatc taggttggaa aaagctgttt ggcaaagaga
       61 gcgacagaaa agaaaaggaa gaagaaccgc tacttcctaa agtgacaaaa ggagaagcgg
      121 tgacggtaga tctacaagtc cttgaaaaag aaacgaagcc accacaacct tataccgaag
      181 gtacgttgat cacagcgatg aaaacagctg gaaaaaccgt ggatagcgaa gaagcacaat
      241 ccatattgaa agaagtagaa ggaatcggta cagaagcaac acgagcaaat attattgaaa
      301 ctttgaaaca aaaggaatat atcaaggtag aaaaaaataa gcttgtggtg acaaataaag
      361 ggattttact ttgccaagcc gtggaaaaag agccgctgct aacaagtgcc gaaatgaccg
      421 ccaaatggga aagttactta ttaaagattg gtgagcagaa aggaacacaa gctactttct
      481 tagctaatat ccaaaaattt gtctctcact tgttggaagt cgtaccggga caaatccagt
      541 caacagattt tggatcaacg ttacaagagg tgaaagcagc cagtgaaaaa caggaagcaa
      601 cgcgtcattt aggtgtttgt ccaaaatgtc gggaacaaga agtcttgctt tatcaaaatg
      661 tggcggcatg tacgtctgaa gcctgtgatt ttaaattatg gacaacgatt gccaaaaaga
      721 aactcacagc cacacaactg aaagagatca ttcaaaacgg ccgaacaagc caatcagtca
      781 gaggactaaa aggtcagaaa ggttcgtttg aagcaacgat cgttttaaaa gaagacttta
      841 caacaggctt tgaattttct gaaaagaaaa aaacgaacta taagaaaaga acacgccgca
      901 ctacaaaata atgaaggggt tatcctatgc gaaatacaac acacaaacaa caagaaatcg
      961 aaaatcaaaa agcaaaaatt cgtttagaac aagagaaatt acgtgcaaat aaaagaagac
     1021 tgcaacaatt aaagaaccag cagcgcattc aaaaagaaat cgaattagat cgatagacgt
     1081 taatcttgtt acaaataaac tactatgata tatttgatgt agtgtatttg taacgaaggg
     1141 aggggaagta aatgcaagat aaactgaaag aattgttggc tattcgcgat ggaaatttat
     1201 ctatgaagga agccaggaaa tttggaatcg cagcaaccac tgtacaaaga ttggtgaatc
     1261 aaggaaaatt gatcaaagtt gatcgtggtt attatgtaga ggatggtcat ggaatagatg
     1321 atttattttg gcttcagcaa cgattttcca ggggaatctt ctctcatgaa acgactttag
     1381 atatttacca gctttctacg aatatgccga aattcattca cttaactttt ccccatggct
     1441 ataacgttga tcgttcaatt acaaaagagc agcagttaca atttcatttc gtgaagaaag
     1501 aagtttatga actaggaaaa gtagagggaa cttcctttca aggaaatcct attactatgt
     1561 atgataaaga gcgtacactt tgtgatattt ggaatccgag atatgaattt gaatatgaaa
     1621 tgaaattaga agcattaaaa gagtacatgg aagacccatt gagagatcca agtaaattaa
     1681 gagattacat gacaaaacta catgtagaaa aagaaatgaa ataccacatg caaagcttat
     1741 attgatggag gaaaaagatg acaccgacac aatttgcagc aaaaacaaga aatttagcaa
     1801 agagtacagg attaaatgca cagttagtgc aacggcattt tatgatggat cgtctaattg
     1861 aacaaatttc tgaatcaaag tacaaaaatg acttcatttt gaaaggcggc tttttattag
     1921 gatcaaaata tggaatcgat agaaggacaa caattgatat cgatacgaca tttagaaata
     1981 gcaaattaac tgaagaaaaa ttgaagagca tttttaatga tttaactaga aaacccacaa
     2041 aagaaggcat tgttttctct attcaaggaa tgaaggaaac aagagaagcc gatttttatc
     2101 ctggatttca aattaaattg gtggctaatt tagagaaagt acgtgtaccg tttaaaatgg
     2161 acgtgacaac aggagattct atttatcctg aagtagatac acactatcac caattaatgt
     2221 ttgaagataa aaaagtggag attccagctt atccaacaga acaaattatt gcagaaaagc
     2281 taagtgctac gttcagcttt ggcacagata attcgagagc aaaagattac tacgacttat
     2341 ttaccattcc gaaactagaa aatatcgata cgaaagagtt atacaaaagt gttcgaaata
     2401 catttacaaa acgtggggat acaaaaccgt tgtcctttta ttatgaaaga gaaatggata
     2461 agatcagagg aaatgactat ttggaaaagc agtgggaaaa atatcgctca gttaacacgt
     2521 ttgcgaattc catttcattt acagatacag tcaacgaaat tgatcaccta atgaaaagtg
     2581 tgatcaaaat tgaaaatcaa gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc
//
LOCUS       DACEPW010000265         2633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.272, whole genome shotgun sequence.
ACCESSION   DACEPW010000265 DACEPW010000000
VERSION     DACEPW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.272"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(187..864)
                     /locus_tag="IW819_002661"
     CDS             complement(187..864)
                     /locus_tag="IW819_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1245739.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGNSL"
     gene            complement(955..1860)
                     /locus_tag="IW819_002662"
     CDS             complement(955..1860)
                     /locus_tag="IW819_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1245740.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            2035..>2633
                     /locus_tag="IW819_002663"
     CDS             2035..>2633
                     /locus_tag="IW819_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1245741.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVT
                     IFTNFFGLATHNTTLFIVLFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWF"
BASE COUNT          727 a          534 c          462 g          910 t
ORIGIN      
        1 tgttatttat aagaaaattt caccagttga gatgattcca agattgtccg tcgatttctc
       61 cttgcagctg cattgttcgt tcgtctatct cagccatcag tttctttatt tcatataaga
      121 gcgcacggtc ttgatattct gatttttcgc ttaattcatc taattgttgg atcagccaat
      181 catttgtcat aatgaatttc ctttcatttc ttgcagtgta aaggtcaagt cttcgatctg
      241 ctcttttgcc tcagtcagtg cttctacgat atatgcttgt ttttcagatt cttcttcctg
      301 cattttctga ttttcctgta ctctttgaat aaaccagtca atccgctgag tcagcgatgg
      361 tttgccttcc aatgagagat actgctgata agcaattagc ttctcccaac gtttttcctg
      421 ggataaatac ataaattgtg ctaaaacgaa tggcggaaga taggtcatat ggaatttatt
      481 tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta tattcttctt ttcctccagc
      541 ttggtattct ttttccgcca cacctgttgt caatacgatt cctagttctt tcccttttaa
      601 tacatgtgct gctcgttcca agacattatc ttgccaaatt tttaaatgag caggagaact
      661 ataccagtaa aacggaaact ggaaaatgat ccgatcatgc tgggccaaga gctgtagttc
      721 agtctctata tctatttttc cgtctggata acttgattcc aaatgatgcc aagtagctgg
      781 taaaccagca gaagcttctt tgaaaaattg ctgatttaaa gattcttcga tcgttggatg
      841 gctaatgatg ataagtgtac gcatcccttt tcctcctttt tagtcttcgc tttacagtgt
      901 agcataaccg ccacaagttt cttttgccga tgcttgtcat tttaaccaga ctaatcaggt
      961 tgtccaagaa actgcggttt gtttttccaa ctgttttttc aaggtatcca aagcaatatt
     1021 cttaaaattt tcgtttttca tacgtttacg atagttcgta gccgtttctc cggtactctt
     1081 tttgaacatt cggctaaata cgactagact attgaaccct acttgatagg caatactttc
     1141 cacggattca tccgtcagag aaagaagaaa cgcccctcga ttgatccgta ccccgatcaa
     1201 atattccttg atactggtac ccatatgatt tttgaatact cgcgacaaat ggctccgatt
     1261 gaccgctaaa tgctgggcga tttcctcaac agacaatttt tcagcataat gtgcctcgat
     1321 gtatttgact gtttcaacag ctaaagagga atgaaccttt tgtacaccac cgctgacctt
     1381 tccgccatct tttaaaagga ctgataaaaa acggtaggtt aatgctgtta actgtaattc
     1441 attttccaag gtttcttccg tatctttcat gcattctaaa ataatgtcga tatatttttg
     1501 tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa agcgactgcc gaatgatttc
     1561 ttctgcagca ctcccaccaa atgaaagcca gcaatacatc cacggatctt ctccatctga
     1621 taagtaaaac gtcgagtctc ctggacgaat caagaacaga tcaccttttt tcaattggaa
     1681 ttgttgattt ttcacatgat aagatccttt gccttccatc acgacatgca ggatgtaatc
     1741 attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt ttagagaaac cacaaaaatc
     1801 aaaatacaat tctttttcta cttgttgatg ttcctttttt aaaaaaatcg attgctccac
     1861 gtctatccct ccttactcct ttagtataat gcaacaaaaa gttaaataaa agtcacagaa
     1921 atatattaaa aaggaagcga tttcttgtta agtttaagtc attaaaagat ctttgttatt
     1981 cgttgtaacc ggtttactta gtattatata ttgaacagga gaggaataca gattatgaaa
     2041 agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt ttggacacga cacattctat
     2101 gttacattaa gcacttattt tatggtattc gtgacaagcc agatgttcgc aggaacagat
     2161 aaggcaacga atgcaaaaat gattgctaca gttacaagct tagttgtagg tatccgctta
     2221 gttgaaatta tttttgatcc tttgattggt ggcatcatcg ataatactcg tacaaaatac
     2281 ggtaaattca aaccatggtt agttatcggc ggcgttgtta gttccgtact cttggtaaca
     2341 atctttacca atttctttgg cttagctact cataatacga ctttatttat cgtattattt
     2401 gcaatcgttt tcatcttatt ggatagtttt tattcattta aggatatcgc tttttggtca
     2461 atgattccag ctttgagtac ggaaacaaag gaacgtgaaa aatttgctac gtttgctcgt
     2521 ttcggctctt ctctaggtgc aaatggtact actctagtcg ttgtccctat cgttactttc
     2581 tttacctatc ttgttactca taaacatatt gaaggcgctt caggctggtt ctg
//
LOCUS       DACEPW010000266         2602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.273, whole genome shotgun sequence.
ACCESSION   DACEPW010000266 DACEPW010000000
VERSION     DACEPW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.273"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW819_002664"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW819_002664"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1245742.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IW819_002665"
     CDS             complement(2260..2511)
                     /locus_tag="IW819_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1245743.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
BASE COUNT          685 a          584 c          471 g          862 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt ga
//
LOCUS       DACEPW010000267         2515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.274, whole genome shotgun sequence.
ACCESSION   DACEPW010000267 DACEPW010000000
VERSION     DACEPW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.274"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..1509)
                     /locus_tag="IW819_002666"
     CDS             complement(43..1509)
                     /locus_tag="IW819_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1245744.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(1820..>2515)
                     /locus_tag="IW819_002667"
     CDS             complement(1820..>2515)
                     /locus_tag="IW819_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1245745.1"
                     /translation="LAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSG
                     YLVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPED
                     KVQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQ
                     PSKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAV
                     LNAMRSLHVKNKR"
BASE COUNT          771 a          523 c          398 g          823 t
ORIGIN      
        1 aagtagcttc tgtcacaacc tcttgtaaca agtatttcta ttttaaggct gaaccactgt
       61 ttctggttca gtaatttctt tgtttttgtc tcgacgagca attgctggca agatcatacc
      121 taataagatc aagacaattg gtgtgatgat attcgtgaat aattggaacc accatgcttt
      181 aggatcagca gtataatcta cttttggcac cattcctagg atacatgcaa acgcagtgaa
      241 ggcaaagcac caagcaccaa agataaaacc gatttttgga tttttaacaa atttatattc
      301 agaattgaat ttcttataag ctttattcaa catcatatat gccaagaaca cccataggta
      361 acgcattggc atgacaactg agttcaagtt cgtcaaccat tttactaatt cattcatatc
      421 tttgatacca aagatcggca acaaaataat gattcctact agtatacctg tcagaagata
      481 accattcacc aatgtacctt tacgattacg tttacgtagc cagtttggta caaattctgg
      541 atccgcatct gctaacaaga tttttagtgg tgcatcaatt gaaaaggcta atgcggagat
      601 ttgtccaatc atgtttgtca atgcgtaaat gatcatcaat gagttaccga taccatagta
      661 attacctaaa atttgaaaag cactgtatgc cccatttgcc atcaagtctt tagggatatt
      721 atcgctagca aacagcatac ccattgcgac tgagcctaat acagcacaaa tccctaccat
      781 tgctgctaag aagatcatcc ctttaggaaa ttcctttgca ggatttcttg tagagttgac
      841 ataaggggat attttttccg cccctccaac tgcaaatact agcatcgaaa cggttgtgaa
      901 ataactgaaa tcaaatgttg gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa
      961 ttccatcgtt ggattgatga acggtgccgt tacagctaaa agaataaata agatcgacat
     1021 gacgaacatc gctgttccgg ccagtccgcc aatcactttc aaagtagaaa gtccttttgt
     1081 tgataaatat aaaaaggcaa gaaatataat aagtgaaata actgctacca tttgtactga
     1141 catcgtattt acgatatttc catttccttg gactgcccaa ccaccagcaa tcagaattgc
     1201 ttgaggcttt tgtgccaaat aaggaatatg gacgacccag taagtccaag cggcatagta
     1261 tgccagtcgt ttattagatg tcgtattttc gacccatgaa ctgacgcctc ctttactatc
     1321 cttgaaggct gatcctaatt gtccaacgat caatgcgtat ggaataaaat aaatggccaa
     1381 aatcaatagc caagaggtga caactgatac tccttgctga gcatagttat taactacatt
     1441 ccccatgccc caaaccatgt taaatgcaat taacgcaaca gtaaaccagc gtaattgttt
     1501 gttgttcatt cgttctctcc tttatgataa aaatttgtta tccagatgta atcatattca
     1561 atttttaaca aacttacaag acagtgttat tttatgtgtg tattctttta tttaatacac
     1621 atcgtttttt catatttttt ccatcaaaaa aataagttat tttcttttat ttcattgacc
     1681 ttattgaatt tcaagctatt ttgaattttc tgcaaaatta aaaacattag ttattttata
     1741 atcctatcta ttttctttac atttttcatt aagaaaagga tataaaaaaa agaggacgtt
     1801 cattcgtcct cttcattgat caacgtttat tttttacatg caagcttcgc atggcattca
     1861 aaacagctaa tactgtcacc ccaacatctg caaatactgc atctcccatt gatgcaaaac
     1921 caaaagcacc taaggaaagt accaaaattt ttatcccaat agcaaagaca atgttctgtt
     1981 tgacgatttt caatgttttc tttgctaaat ggattgcttc tgcaatcttc gatggttgat
     2041 cattcatgat gactacatct gctgcttcaa ttgcagcatc gcttcccaat ccacccattg
     2101 cgattcctac atctgctctt gctagcaccg gtgcatcgtt gattccatct ccgacaaatg
     2161 cggtcttttt attgttactt tgtaagattt cctctaaccg ctgcacttta tcttctggaa
     2221 gcaattcgct atatatcttg tccacgccta cttgcttccc gatatgatca gcaatttttt
     2281 tcgagtcgcc cgtcaacatg actgtgtttt tcacaccagc ctgttttaat tgaaccaatg
     2341 cgtctacggc atcttctttc aaggtatctg caataacaag ataaccactg taactttgat
     2401 cgatcgctag ataaagcaat gtaccgattt cttgacttga aggaaaagag atttcaaatc
     2461 tttccatcaa tttgtgatta cctactaata cttgtttgcc atcaacttca gctaa
//
LOCUS       DACEPW010000268         2509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.275, whole genome shotgun sequence.
ACCESSION   DACEPW010000268 DACEPW010000000
VERSION     DACEPW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.275"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            344..1924
                     /locus_tag="IW819_002668"
     CDS             344..1924
                     /locus_tag="IW819_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1245746.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2122..2346
                     /locus_tag="IW819_002669"
     CDS             2122..2346
                     /locus_tag="IW819_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ1245747.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          893 a          404 c          530 g          682 t
ORIGIN      
        1 ttttagtcag ctgacaaaaa aatatttgac tcttatggtt tggtggcttt ttcattcgct
       61 gaatccatgc tatactgatt cagttgtagt agttgacaaa ggttgtgaaa aagctatcct
      121 gcattaagta ataagagcag ccaagcaaaa acagcttttc gtatttttgg cttgggtggg
      181 cttattgctg ataggctgtc tctagaacgc cgtttactcg gaaaaagaga ctgctacacg
      241 gcttttgtca cagccctata tgaaattgtc tttctttaag agatcggaca atctgtgcct
      301 agagaaaata taaataattg aatggaagaa atggagttag gttatgaata atccaaaatt
      361 aaagcaacaa gtggacaatc gcagaacttt tgcgatcatt tcccatccgg atgctgggaa
      421 aacaacgatt acagaacaat tactattgtt tggtggggct atccgtcaag ccgggaccgt
      481 aaaaggcaaa aaaacaggta attttgccaa atcagactgg atggaaatcg agaaacaacg
      541 tgggatttct gttactagtt ctgttatgca gtttgattat caagggaaac gagtaaatat
      601 cttggataca ccaggacatg aagatttttc tgaagatact tatcgtactt tgatggccgt
      661 ggatagtgcg gtcatggtaa tcgatagtgc aaaagggatc gaagcacaga caaagaaatt
      721 attccaagtt gtaaaaaaac gtggcatccc gatatttact tttatcaaca aactagatcg
      781 tgatggtcgt gaaccattgg aattgttaga agaattagaa gagctattag atatcgaatc
      841 ttatccgatg aattggccaa tcggtatggg taaagggcta gaaggtcttt atgatatcta
      901 ccataatcgt gtagagtttt atcgtccgga aaattatcag gatgaacgat tagtagaatt
      961 ggatgaagat ggactgcttc ccgaaaatca tcctctaact gaaaattcct tatatgaaca
     1021 agtcttagaa gaagtagaac taatcaaaga ggctggagat gcatttaatc aagaaaaaat
     1081 tgctcgtggc gatcagacac cagtattttt tggttctgca ttgactaact tcggtgtaca
     1141 gactttttta gagactttcg ttgaattggc accagctcct tatggacaca aaacacaaga
     1201 tgatcagatg gtaagtcctt atgaggaaga attttcaggt ttcgtattca agatccaagc
     1261 aaatatgaat ccagcccatc gtgaccgaat cgcttttgtc cgaatctgtt ctggaacatt
     1321 cgaacgagga atggatgtct ggttggaacg tacaaataaa aaactgaaat taagcaatgt
     1381 cacacaattt atggcagatt cgagagaaaa cgtcgaaaaa gcagtagcag gcgatatcat
     1441 cggtgtatat gatacaggca attatcagat tggggataca ttgttcgaag gaaaactaaa
     1501 agttgcttat gaagaactcc cttcatttac tccagagctg tttatgaaag tgactgcaaa
     1561 aaatgtcatg aaacaaaaat ccttccacaa aggaatctat caattagttc aagaaggagc
     1621 gattcagctt tataaaacat atctaacgga agaatatatc attggagctg ttggtcagct
     1681 acaattcgaa gtattccaat atcgtatgtt aaatgagtat aacgccgaag taatcatgtc
     1741 tccaatggga cacaaaatcg ctcgctggat cgatccagaa gatttggatg agaagatgag
     1801 ttcaagtcgc aatatcttgg ctcgtgaccg tttcgaccaa ccgttgttct tattcgagaa
     1861 ccaatttgca gaacgctggt ttgcggataa atatcccaat gtaaaattaa aaagtttgat
     1921 gtaaagaagt cttgacacaa gctgccctgc atcaagtaat aagaatagcc aaacaaaaac
     1981 agcctctcat attttgttta gagccgtgac ggattttccg tgacggctct ttttactata
     2041 ttaaaatcat agaaatgtct aatcaaaaaa atataaaaat tattttgcgt acatatgatt
     2101 aaagaaagga gagtgtgaca catggaaaca gaatggcaaa gacgtgaaag aaaaaaacag
     2161 aaaaaagaag agatgttacg attatgtcga ttattagatg taaaagactg ggacaaaaac
     2221 gagcaaattg cacaaaaagt acgatcaatc atccataaag aggaaaaagt taaaaataaa
     2281 gacacgcgta gaactgaaaa tgaaaagaga aaagtacaaa agaaaagaga aaaatcaaaa
     2341 agctagaaag aaaaagaggc tgagacagaa gcgtttaact ccaagaaata agctggaatt
     2401 cacaaaaatt tgaaaagcaa ttttcgtgaa tttcagctta tttccgaagg agttgcttct
     2461 gctttcgccg tttatacgtg tttagagcgt gagacaaaag tgttcttta
//
LOCUS       DACEPW010000269         2497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.276, whole genome shotgun sequence.
ACCESSION   DACEPW010000269 DACEPW010000000
VERSION     DACEPW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.276"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..1249)
                     /locus_tag="IW819_002670"
     CDS             complement(26..1249)
                     /locus_tag="IW819_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1245748.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(1266..2345)
                     /locus_tag="IW819_002671"
     CDS             complement(1266..2345)
                     /locus_tag="IW819_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1245749.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT          711 a          513 c          413 g          860 t
ORIGIN      
        1 ttttttcata aaaaatgttt gaattttatc ttttaaatga atttaatttt attttgattc
       61 gttcaatcga gtcttcttct tcttttcttc gcatctcttc ttcatgataa acaaccatgt
      121 cttgataaaa ttcgatcata gcttcgccga aatgtgtaga tgaaatatca taaagtttag
      181 cttctaaaac ttttggatct ttaggaactt cactggaaat ataatcaata agtgctggtg
      241 caaagtcttc gtcggtttca aaagttttcc ctaatgaagg atgatcgaac aatcggttca
      301 aataagcatt tccttccacg acacaaggtg ttccggctgc catcgcttct gtataagtaa
      361 gcccttgtgt ttctgaagta gaagcactga caaaataatc tgccgcttta taataaagtg
      421 ctacatcatt gttgtccact tctcctgtga actgcacaaa ttcttctaac tgcagttctg
      481 agatcaattc ctctaactct tctacataag gtccttttcc tacgataact aatctggcgt
      541 taggataagc ggagatgacc tgaggaaacc cattaatcaa ggcttggata tttttctcat
      601 aagaaatcct gcttaaagaa agaatcatca aatggttttc ctgaagacct agtgactccc
      661 ttaatttgct gatatcctgc tgagtgatat cagaacgttt gaatttttca atgtcgatac
      721 cagtaggaat cacgcgaagc ggtgctatta caccatagct gcgtaatgta tcaataacac
      781 gttcacttgg acaaactaca cctgtcgtat gatttgcaaa taatctagac aagtatttta
      841 catgggattg cctgatcact tttcctttac cgatataatg aaggtagtct tcatacatcg
      901 tatggtaagt atggatcaga ggtattttca acttcttagc taccatcttc ccaagtatcc
      961 ctgctccgaa ttctgtatgg gtatgaataa gatctagttc tagttctttg gcaaccaaat
     1021 aagcatacca catgcctcta acaacaactc ttcgatcctt gaaagaaata aatggtacac
     1081 ttggcattcg aatcacatct ttttccaact tatccgcatt aggatcggtc gttgtaaaaa
     1141 tatagacttc atgcccatgt ctttctaatt cttgttttaa tgttttgatc gatgtagcaa
     1201 ctccgctgac ttgcggaaag taggtatcgg taaaaaagcc gattttcatg tttttcgcct
     1261 ccaaactagt ccttgacctg atttactttt tttaatttgc tagagacttt ttcattcagt
     1321 acactttcgt atacattttt aagttcttca ccgattttat tgatacttcg ctcttttgct
     1381 gtttgataac cggcttgtgt cgtactcggt agttcttgat tggtaatctg ttggatcagt
     1441 cggataaatt cttcgtttgt cttccccata tagcagttct tgctatcttc taaccagcca
     1501 ttatacactg gaatatctcg aacaagtacg ttttgtttgc ttgctaaagc ttctaaaaca
     1561 acgatccctt ctgtctcttc ataagaaggg aaaaagaata gatctgcacc tgaatacgca
     1621 ccttctatga tctctccacg aatgtaacca ggaaacagga cattttcagg atgatcttct
     1681 tttacaattt tccggatgtt ccgaggaatc gagtacatgg gaacatgacc aaaccaaata
     1741 aatgtgtatt cgggcatctt cttagcaatt tctacgaaat caatgattcc tttacgttcg
     1801 aaaaacaaac cgacacaaat gattactttt tgatccggag acagtttgaa atattctctg
     1861 aacttttgtt ctttcattgg atcagggtga tatttctcta agtcgatccc attggaaata
     1921 gcttgaatcg gcacacggat accatagctt tccaacaact gttttgaata aggtgttggc
     1981 gtgatcagat agtccgcttt ttggtacaat cctaccagat atttttttac gataggagaa
     2041 agctggttcg atccgataaa ggaatttcgg aaatcttcct ctgtagaatg cgcatgatag
     2101 acaacttttt ttccttctct tctggcttta ttgaccatat tatgactatt gattccgtat
     2161 gtattgatgt gcaggatgtc ataatcttct ttttcatcta gtgtataaga gataccgact
     2221 tccgtcagag cacgtttttg atgatcaagc gcacgcccga ttccagattt tgcaagcatc
     2281 ttttcacttt caaaatataa taataccttc accttacgtc ccccttcact tatatagtat
     2341 aacatagcct acaaactgtc actcaaactt aagccctatt tttgtctatt cttactgaca
     2401 aaaacgtttt cttaaaaaag aacaaaaaaa ctggcgtagc tcttttacaa gccacaccag
     2461 cttttgatac gttgtttatt ttacatattc atgaaca
//
LOCUS       DACEPW010000270         2472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.277, whole genome shotgun sequence.
ACCESSION   DACEPW010000270 DACEPW010000000
VERSION     DACEPW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.277"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..911
                     /locus_tag="IW819_002672"
     CDS             <1..911
                     /locus_tag="IW819_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1245750.1"
                     /translation="KKSVKIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIV
                     MQAMDQTAAGNLETRIDITNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHM
                     RALQAQINPHFLYNTLEYIRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEEL
                     DFCEKYVYLYQMRYPNRVAYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNA
                     LSVKVLQEGKRVRIIIKDNGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRAS
                     FGSSYYMSLENNETGGLTVSITFKEG"
     gene            908..2359
                     /locus_tag="IW819_002673"
     CDS             908..2359
                     /locus_tag="IW819_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1245751.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT          916 a          399 c          500 g          657 t
ORIGIN      
        1 ataaaaaaag tgtaaaaatc gaaacgggaa aaaatcttgc cccgctgatc attggtatca
       61 ttattttaga caatatactg cttatctttc tttataaaac attcaaacga tattcacagc
      121 aagtatctat cgtcatgcag gccatggatc aaacagcagc agggaattta gagacgcgga
      181 tcgacataac aaacacggaa tatgaactga aggaactttc aatcggtatc aacgaaatgt
      241 tggatagcat caatcagttt gtagaagaca tctacaaatt agaaatcaaa cagcaagacg
      301 cacatatgcg ggcgcttcaa gcacagatca atccacactt tttatataat acattagaat
      361 atatccgaat gtatgcaatc agcgaaggaa gtgaagaatt agcagatgtt gtgtatgcgt
      421 tttccgcttt gcttcgaaac aatacgaatc aagagaaaac gatcacttta aaagaagaac
      481 tggacttttg cgaaaaatat gtctatttgt atcaaatgcg ttatcctaat cgagtagcct
      541 atcattttat gatcgatcct gatttggaaa agatcgaagt acctaaattt gtgatccagc
      601 cattagttga aaattatttt aaacacggta ttgattttac tcgattcgat aatgcgctaa
      661 gtgtcaaagt cttgcaggaa ggcaaacggg taaggatcat catcaaagat aatggaaaag
      721 ggatgactga aaaacggcta aaacagattg aagaaaaact gtctcatcct aaagtggaac
      781 ttcacggttc tatcggtctg caaaacgtga atgaacgttt acgtgccagc ttcggcagca
      841 gttattatat gtcgcttgag aataatgaga caggtggact gaccgtttct attacattca
      901 aggagggatg agcgtgtatc aagtgttatt ggtagatgat gaatacatga ttttgaatgg
      961 gttaaaaaag ataattgatt ggcaaagttt aggctttcaa atagtagcaa cagcagagaa
     1021 tgccaaagaa ggactagctg tacttgaaca gcgacagatt gatttagtag taacggatgt
     1081 gacaatgcca gaaataaatg gactggaatt tattgaagcg gctcaaaaag aacgtcataa
     1141 ttttgaattt atgattttat caggatacca agaattcgat tatttgaaag gaggtatgca
     1201 attaggcgcc gtgaattatt tgatgaaacc cgtgaataag ttcgaattgg ttgaaagcct
     1261 caagaaaatc aaaacacggt tagatcagca aaatgaacaa aaaaatcaac aagaaatcta
     1321 tcaagaactg ttattcagtc aatggttgaa tgaagaattg gatgaaataa gtgaagaaga
     1381 aataatagga caaataagtg agaagcagca taggatagta ttgattcaac tggcaagaac
     1441 tcatgatact cttatcaata gatggttgca agaacggcaa caaatctttt actatcaacg
     1501 aaattacgga gaccgtgtat tgatcacgtt gttgttggag gcagatgaag ttgatggctt
     1561 ctgttgtttt gttcaagaaa actttcctga tcaggaatgg ctgatcagta tcggggaaga
     1621 gacagattct ttggacaaaa tccctgaaac attccaacaa gcgaaagaca atctacagtt
     1681 gcatcaattt tatggagata aagacgagca tgttttttac gcagtacaag cagaaacaaa
     1741 agatcaaacc atcgattttt caagtttcag acgcgtgctg caaaataaac aattggaagt
     1801 cgctcaaaaa atgatcactg atttttttga acagtttcag ttagcagcca tgatgccgga
     1861 agatatacga tattccagtt ttttactgtt tatggagatt caacgggaat tgattgatct
     1921 ggaagatgag gaatatttac aaggaatcga aaaaatccat caggcaaaaa cggtgcaaga
     1981 acttcagcag ctacttctgt cattcatcaa aaagcatcag agacagaaaa agtacagtga
     2041 caatgtggaa aaagtaatcg taatcctgca ccagcattat caagaaccac ttacactcaa
     2101 agaagtgtca gaaaacctgc atttaaatgt catgtatcta ggacagctgt tcaaaaaaga
     2161 aacgaaaaaa agtttttcag cttatttgaa ccatttacgt atggaaaaag cgaaacaact
     2221 gcttttgcac agtaatcaaa acatcaatga gatagcaagt gagattggct acaacaacac
     2281 cacgtatttc tctaaattat tcaagaaaat cgtcggcaga tcccctaaag agtaccgaga
     2341 aaacagtgga gaatcataaa aaaaaaagcc aatgtaatga aataacttat aaaaaattgt
     2401 ctagaaatct aattttgggc tgttcttatt acttgaggca aggcagcttt ttcatgacct
     2461 ctcgataaac gg
//
LOCUS       DACEPW010000271         2465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.278, whole genome shotgun sequence.
ACCESSION   DACEPW010000271 DACEPW010000000
VERSION     DACEPW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.278"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            135..515
                     /locus_tag="IW819_002674"
     CDS             135..515
                     /locus_tag="IW819_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1245752.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            497..1411
                     /locus_tag="IW819_002675"
     CDS             497..1411
                     /locus_tag="IW819_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1245753.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            1371..1649
                     /locus_tag="IW819_002676"
     CDS             1371..1649
                     /locus_tag="IW819_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245754.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            1656..2345
                     /locus_tag="IW819_002677"
     CDS             1656..2345
                     /locus_tag="IW819_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245755.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
BASE COUNT          932 a          470 c          478 g          585 t
ORIGIN      
        1 aggtatttgg ctagcaaata tagagcttgc caagcccttt agtgagcttt agtgagcttt
       61 acttattgta cctttttagg tacaatggta ttgcgaacga tacgtccgta ggacgtactg
      121 aaagggtggg acttatgagc gaacaaaatc aatatttggc tagcaatcat tctaaaaaat
      181 atacctatcg atccgagcca aaacaaatca gttttcgagt gagcgaatcc gaatttgcaa
      241 agctgaagca gtcagctgaa gctttgcaaa tgagtgtgcc ggctttcgtg aaagccaagg
      301 cacaaaacgc gcgtctggtg acaccgaaag tggcgccaga cattgctcag gcaatcgcgc
      361 gcgatttagc caaagctggc gggaatatca atcaaattgc caagtggtgc aacacacacc
      421 aacacgacgt tgatcctggc gacgcccaac gcttatccga aaacctaaaa atcatgcaaa
      481 aggagctaca aaagatatgg caacagttaa agtaagccga accacttctt gtagccgagc
      541 gatcaattac gccgaaccac gagcaaccgt caaaacaggg ataaactgtg acattgatta
      601 tgccaaaagc gaaatgaaac aaattcgcat gctgtatgga aaagacgatc gtgtacaagc
      661 acacttattg atccagtctt ttcgaccagg agaaattacc gctgaaaaag ccaatcaact
      721 aggggcagag tatgcgaaaa aaatagcccc tgaccaccaa atcgcgattt acacacatac
      781 agacaaagac catattcata accacattgt cattaactcc gtcaatctgg aaactgggaa
      841 caaatttcaa gcccatggtc aagcattttt aaacaaatgt tatgacctaa atgacgagat
      901 ttgtcttgaa catgacttaa caatcactga acgagggaag aaacccgaaa aacgaaccat
      961 gtctgaaatc aaattaaaag aaaaaaatga acctgtttgg aaagacgaaa ttcggtttgc
     1021 gattgatcaa acaatgaaaa acaaaaaaac tcgaacgtat aaccaatttt gtgattctct
     1081 taaaacattc ggcattcatt gcttgaatcg tgggaaaaat tttacttacg aattgatcca
     1141 caaaaagaaa aaagtacgat ccaacaaact aggaaaggat tacgaaaagg agacgatttt
     1201 acatgagctg gacagacgag aacgagccta caatcaacaa cgaaccaacg agctacgaag
     1261 agctcgagct agcattcgcg aaactgcaaa agaagtacga agagaacaac gaactcttac
     1321 gactgaacaa ccggaaatta gaccgactcg aagaaaccat gaccaacacg ttgaaagagg
     1381 agcagactac caacaagaat tatcgttcta aaacgttaca agacatagaa aatgaaataa
     1441 agaaacaacg tgcttttctt gcaccttata ccaaagaaag aatacaaact acaattgatt
     1501 cacaagtcaa aaaattccac catgcgctat taaaccagcg aaataaagaa cagaaaaaag
     1561 taattatgca gcgatcaatg gatattcttg cccccttatg ttgcttaatc atgacactgt
     1621 tagccttttg gctggtatgg ggagaataaa tagaaatggc attctattta ccctatctac
     1681 tcatttttgc cagtatttta ggaattattt ggcttagcta taaggcttat caaacaagat
     1741 attcattaaa aggctctaaa attcgattta agaggttttt attgccaggg gttatattta
     1801 cattatcaat cgttttaagt ggtttattcg gcgttttaga gggaaataaa cgtatttcaa
     1861 gaagtatttt attaggggat atcactcaaa agtatgaagc agcatgtgat aaaaagaaaa
     1921 aggagcaagt gttaacacaa aaaatggaag agtttaccac ttgttatgag gaaatgaacg
     1981 atatttttgt gaatcaagaa aaacgtttga cggataaaaa catggaaaaa ctcactcgtc
     2041 tttatcaaaa cttgccagaa aaacagcaaa aagaggttca agacaactat gagcgaacca
     2101 aaaaagacgt gcaatatttt aaagatacgc agacagaaga agcttgttat aatttattca
     2161 gtgatacaat ccctttttca actagtgaac aggaacgaaa agaacggcaa caatcagtta
     2221 cctacgaacg ttataaagca ctgcttcagc aagcaacgaa catacaaaat ccaacgaaaa
     2281 aagaaacggc attaaattat ctaaaaagtg tcaaggaatg gttagatcag caacagcaaa
     2341 attgaatttg atcggcaatc atgttaaact aaacatgcaa atgatcaaac attagataca
     2401 cgaaagcccc agagaggtcg gattctctgg ggcttttttt caggttgatg tcgcctttat
     2461 tgatt
//
LOCUS       DACEPW010000272         2422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.279, whole genome shotgun sequence.
ACCESSION   DACEPW010000272 DACEPW010000000
VERSION     DACEPW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.279"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..140)
                     /locus_tag="IW819_002678"
     CDS             complement(<1..140)
                     /locus_tag="IW819_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1245756.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YI"
     gene            750..1709
                     /locus_tag="IW819_002679"
     CDS             750..1709
                     /locus_tag="IW819_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1245757.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1696..2301)
                     /locus_tag="IW819_002680"
     CDS             complement(1696..2301)
                     /locus_tag="IW819_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245758.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
BASE COUNT          810 a          395 c          363 g          854 t
ORIGIN      
        1 tgaatatatt ctgttcgttg attttccatt agttgattaa ataccgttgt taaaatattt
       61 ttagaaacgt catcctttac agaatattca ataatgcttt gaatctcttc gcttttcagt
      121 gtaaaatgta cttgggtcat gtaaaagtcc tcctgggtat gtttttgtcg ttaaaaacat
      181 tgtaccgtaa aaggactgtt atatggcctt tttactttta cacaattata cggactttat
      241 ctaaaataga aaaatatgaa tatattttaa atattaagat gagagtcatt aacttatgat
      301 agtagtcaat ttgcttttca ctaattacaa aatatgtgat ataggaagtt tagttattgt
      361 tctcataatg ccatcctgtt tttagattat ctagtaattt ttttgttatt taaaattagg
      421 agcagtgcca attgttttaa ttttaaaggc tttttttata tcttctaaat tagaagatat
      481 ttgtatatca atatggaata tattaccttt tttgtatttt tttatgttat ctttagctaa
      541 aacatcaaat acatgtcctt gaaattgtat cgaggcagct ggatttagtt taaattgagg
      601 aaactttacc acagctgatt caaaacactt gtcacatttt tttatttttt taccttcgcc
      661 gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca aaatagttcc
      721 gtcgaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca ccaactgaac
      781 tcgtaatgat agaagcttat ttcaatcaat cacactctgt ttcaaaagta gcccatctgg
      841 tacaacgttc tcgacaaacc atttataatg tctatcgttt tcttaaatct ggtggaagcg
      901 caataagcta ctatgaacaa tataagaaaa acaaacgtaa ctgcggaaga cgtccgattg
      961 tgttaccaga tgggcaacaa gaatacattc aaaaaaaggt ggctcaaggg tggactccag
     1021 atgtccttat tggtcgagca gagtttccta ttgcttgttc ggtacgtaca ctatatcgga
     1081 tgtttaaaga taaaaccttt gaccctcacg atttaccaat gaaagggaaa agaaaaccaa
     1141 acggacataa agaaaaaaga ggaaaacaag cttttcgccg ttcgattcag gatagaacaa
     1201 ctgattatat acagttcaat catgagtttg gtcacctaga aggtgacacc attgtcggtg
     1261 gaaaacataa aagtgcggtg atcacgttag ttgagcgatt atctaaagtg atcatcacct
     1321 tgaaacccgc tggtaggcaa gctatagata ttgagaatag actgaatcaa tggtttcaat
     1381 ctgtaccgaa acacttgttt aaatccatca catttgattg tggaaaagag ttctcgaatt
     1441 ggaaaaagat tagtaatacc aatgatattt cgatttattt cgctgatcca ggaacacctt
     1501 ctcaacgagg actaaatgaa cattcgaatg gcttattacg taaagatggc ttatataaag
     1561 ctatggattt taataaggta tccgaaacct ttatccaatc ggtggcatca aaacgaaatc
     1621 atattcctag aaaatcatta cactatcgca caccattgga agtatttttg agttacgtag
     1681 gtaaagatga tttgtctagc ttaatttgac aaacgaaact ttgtaaaaat attcgattga
     1741 atattctatt tggcacgact cacttggcag tatatgtatg tcaatatttt tcatctgttt
     1801 cataggtatt tcaaaatcta aactattaaa aacatataat tccctgtaat tatttacatc
     1861 taactctact aaatttatct ttggaacaat agatcctcca cccattccac cataataaaa
     1921 aaaatcttgt tctctatcaa aatagcacat attaagctca gtttgtttat catcctcaat
     1981 aattttataa atcaaaccta tataatcatg aatgctggca aaaatttttt ttaagtcaag
     2041 ttctaaatct tcttcgaaaa cacatttaat ttctcctact gataattgat actctatatt
     2101 acatgactta attagttttt tagatccccc ctccagatag gcatatgttt caataattat
     2161 gttcttttta ttgcttggca aatttccgtt atttattgca taaattaaaa atgactgtct
     2221 atttagccca taatctaata caacttcttc atactcatat ttctttaaat atttaatatg
     2281 aaaatatgct gaattaatca aaactcgagt gttactgttg ttatttattg ttgttctatt
     2341 gtgtataaaa ttgtataggt tgtcatattt tcttggttta gtgaactact caccacttag
     2401 ctaaacctaa cggtttctta cg
//
LOCUS       DACEPW010000273         2404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.280, whole genome shotgun sequence.
ACCESSION   DACEPW010000273 DACEPW010000000
VERSION     DACEPW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.280"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            679..1905
                     /locus_tag="IW819_002681"
     CDS             679..1905
                     /locus_tag="IW819_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1245759.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(2013..>2404)
                     /locus_tag="IW819_002682"
     CDS             complement(2013..>2404)
                     /locus_tag="IW819_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1245760.1"
                     /translation="GNPGKYYYSLQLPPFAPPSWIFGPMWTLLYILMGISLYLLLNHN
                     NKKQRNNLVGLFVIQFIFNFIWSALFFNLRNIFIAAIDITLLVIFLSVLMYQLWLHHR
                     LAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT          885 a          354 c          484 g          681 t
ORIGIN      
        1 ggctaagtaa atgcatttgt tcacttgaca tgtcctaact aattatcaag caaggaacat
       61 tttcttcgtt agtaatggat tttaaagaaa tttcagtttt tggatgttga ataaaagacc
      121 atttgtgaaa ccactagtat acatactcat taccatgtca attctattac tttaaaagaa
      181 aatcgtttta ctttgaagag gagcatatct atccattaaa agatgatggt aatgcaatca
      241 ctagttcgag tcattttaag agccatgcag tgagctggaa ataaaacgaa tagaagatgg
      301 gtttaacaaa aggaccagtc ataggaagaa attgtcgtga aatcgaatga taatctgaac
      361 ggtaggcaaa gactgttaaa aagatgggaa aggtatgtgc gatcatttta ctgcacgatt
      421 atttggaaaa taccttgttg ttcgcttttt ctggaaaaaa tacataaaaa aagataaaac
      481 gattgcgaac tgcgtgtatt cggtctttcg atctttttca cggtatggta tgatagttaa
      541 gaattttgag aaattttaaa aaataataat cgttacacaa aaatgttttt taaagttttc
      601 ttatgattaa aaagtgaatt tgctgattga acagttatat gagacaatat tttagaaaat
      661 gtgaaacaga ggtgtattat gaagcgatgg caaaaagttc tattaacctt gttaggggta
      721 ttcgttttgc tgattgccgg attatcagca tacggaatca gatttatggg agaagcgagc
      781 caaacagtaa acaaaatttc taaaaactct aatcgaatat cctccaaacg ttctgagaga
      841 gtaagcattg atgacaaaga acctttttcg gttttgctat taggtttaga taccggtgga
      901 ttaggaagaa cggaacaagg acgttctgat acgatgatgg tagtaacagt taatccgaaa
      961 caaaaaaaat caacgatcat cagtttggac cgagatattt atacgaatat cgtaggttat
     1021 ggaacggtag ataaattaaa tcatgcctac gcatttggtg gtgtcgaaat ggccatggat
     1081 tcaattgagc aactgctcga tattcctatt gatcattatg taacgatcaa tttggatggc
     1141 atggaagatt tgatcaatgc ggtcggtggc atcaaagtga ataataaaat tgactttact
     1201 ttggatggtg tccatgtacc agtaggtgaa caaacattga atggagaaaa aggtttagcc
     1261 tattctcgaa tgcgacacga agatccagaa ggcgatatcg gtcgacaacg tcgtcagcgg
     1321 gaagtagtga cgaaaatcgt caataaagtt ttaagtttag atggcgtaag caattataga
     1381 aaaattttaa aagcagttga gaacaatgtg acgacggact tagattggga tgacatgctt
     1441 gatgtagcga cgaactacac cccggcgttt gaaacaatca aacaagacca gttgcaaggc
     1501 gaaggacaaa tgatcaatga tatctactat caaattttag gaaaaaatga tcttctttcg
     1561 atccaaaatg aactgaagaa acaattaaat ataaaaacaa gcgatacatt acctaatttg
     1621 aaaaatgaga atgcttcaat gatgttcttt gatgattcgg aagatggcga ttcgacaaac
     1681 ggtgatggat cgaatagtga agggtacacc gattattctt cctattcaga aaacacatat
     1741 gatcagtacc aagatccgaa tacctattat cagcaaacag aagatacaag tcagtactat
     1801 acaccaagtc aagatactca aagttatgat ccttacgctg gacaggaaca aaatggttat
     1861 ggttatgacc agaataatga gtatggaaca acaagtgggt actagaaaaa aatagtttgg
     1921 ttaataaaga gtgccaccta aattagacaa aaaatctaac ttaggtggca ctttttctat
     1981 gtgttaaatt acaggacgtc tctttttagc gcttagttta aaaaataaat tgaataattc
     2041 aatagagttg cgaatagtac ccataaataa taaggaatca tgagccacat tgccaggcga
     2101 tgatgcagcc acaactgata cataagcact gacaagaaaa taactaaaag tgtaatatcg
     2161 attgctgcaa tgaaaatatt tcttaaatta aaaaatagag cagaccatat aaaattaaaa
     2221 atgaactgaa tcacaaaaag accgacaaga ttgtttcgct gttttttgtt gttgtgatta
     2281 agtaacaaat aaagagaaat acccatcaat atatacagta aggtccacat cggcccaaat
     2341 atccagctag gtggtgcaaa tggtggaagt tgcaaagaat agtaatattt tccgggattt
     2401 ccgc
//
LOCUS       DACEPW010000274         2397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.281, whole genome shotgun sequence.
ACCESSION   DACEPW010000274 DACEPW010000000
VERSION     DACEPW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.281"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..155
                     /locus_tag="IW819_002683"
     CDS             <1..155
                     /locus_tag="IW819_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1245761.1"
                     /translation="EKIKLLLPDTPISFIPHEEMKKELNNCHAFIRTGEMTPYANIIL
                     VSGVVF"
     gene            155..1528
                     /locus_tag="IW819_002684"
     CDS             155..1528
                     /locus_tag="IW819_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1245762.1"
                     /translation="METTMNPEQMVLYEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRDNMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            1885..>2397
                     /locus_tag="IW819_002685"
     CDS             1885..>2397
                     /locus_tag="IW819_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1245763.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNN"
BASE COUNT          712 a          454 c          489 g          742 t
ORIGIN      
        1 tggaaaaaat caaattattg ttaccagata caccgattag ctttatccca catgaagaaa
       61 tgaaaaaaga gttgaataac tgtcatgcat ttattcgtac aggcgaaatg acaccttacg
      121 ccaatattat attagtgagt ggagtggtct tctaatggaa acgacaatga atcctgaaca
      181 gatggtactg tacgagcaga ttcatatgct gattgaacag ttgggaggaa aagacaatat
      241 tgattctgtc cttccatgtg cgactcggct tcgtatcaaa ttgaaaaaac agccagtagc
      301 caatgcagaa aaattaaaac agcaaatccc ttccattaag tatttcattt tactcgatag
      361 atatgcagaa ataatcatgg atcagggatt ggctcctgct tacaaggaat tgacgcgtca
      421 aatcgatcat tcagctccga aagaaaagct aaatccgatc gaaaaactat ttggtttgat
      481 atcggaaatc tttacgccga tcattccgct gtttgccggt tctggtatat taaaaggtct
      541 ggttgtttta gcgactacga ttggctggct tagtgaaaca agcggaactt atcatatttt
      601 gtctgctgcg tcgaatgccg tcttttattt tctgccaatc tttcttgctt tttctgccgc
      661 caaaacgttt aaagtgaatg gatatattgc ggctgtcatt gctgcagcac tgattgaacc
      721 gaatctgacc tctttacttt cagacagtgg caaagcagct actgattttc tggggattcc
      781 tgtagtactg atgcagtata cgtctactgt catgccggcg attttaggta ttttctttta
      841 ttcttttgtt gaaaaattcc tcaaaaaata tattcgcgat aatatgcaat tagtatttgt
      901 acctttactt tcattagtta tcacggttcc tttgacgtta atggtattcg ggcctgcggg
      961 tgtttacctt ggcgaaggtt tagctgccgc aattacttgg atgatggata ttaacggacc
     1021 gatcagcggc gcaatcatcg ctggtggttg gaatattttg gtaatatttg gtatccaatg
     1081 ggctgtaaac cctgtcatga tcagtaatat ctctgcttat gggttcgacc gaatcgttcc
     1141 gttgacagga gcagccaatt ttggaatggc tggtgcagct ttaggtgtat ttctacgaag
     1201 taaacgttca aaaactcgtt cgatctccgg ttctgctttt gcttcgattt tacttgcggg
     1261 agtgaccgaa ccaactgttt acggaattgc gatcccattg aaaaaaccat ttgttgctgc
     1321 ttgcattggt gcagctgctg gtggagctgt aatgggattt gcccaagtga aagcgatcgc
     1381 ttttgtattt ggtagtctga ccacattgcc tgcattcatt tccggtactt ttttctggta
     1441 cttagcagga ctggctgtta gtctagtcgt agcaatgatt actactctcg ttagcgggtt
     1501 tgatgaagac ctaatgtctt atgaatagtt aatgaaaaag actgaagtat gagatgttta
     1561 tcacatattt cagtcttttt ctttttagga tcattatttt tataaatttt tgtgatactt
     1621 tccaaacttt ggtataattt acatatgcat aaccattttt atccccactt gtttatcccc
     1681 aaacaaaggt ggtctatgca ataaaatgaa cgtcaaagaa aaacgaatga atgctcgttg
     1741 cttatttaca aattgtttta tttagaatac acagacagat ttatgaaaag aaaaagcttt
     1801 tcgacacgat tatctgttcc aaaaaatgtc taatgaatgc tgaataagac agaaaatcaa
     1861 tgaaaggaaa aggactagtt tattatgaaa aataaaaaaa cgatacgttt gctagctgct
     1921 acttggctga ttgctccgtc cgttttgtcg accaataccg tatttgccgt agataatcaa
     1981 acagcagatc aaacagtcac aacagatacg actacatcca gctcagctac tagtgaaaca
     2041 acggattctt ttacagaaga cccaactgga acaactaact caacaacagg atcgaccgag
     2101 ccaactgaaa ttgatccggc tgatccatca gaaaatgaaa agccagccga gacatattcc
     2161 cttacaattg ctccttcttt gattggtaag atccaactga ctgcttcgac agcggattcc
     2221 cctgaacagc aacctttgga tattacggaa agtggaacgg tagaaggttt attagcggaa
     2281 acgaaggtaa cttacactat tacgccagct gaaggtgaac aaattgcttc agtcgctgtt
     2341 aatggtgcca aagaagtaaa ttatgatgca aagaccttta cagtaggtac gaacaat
//
LOCUS       DACEPW010000275         2389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.282, whole genome shotgun sequence.
ACCESSION   DACEPW010000275 DACEPW010000000
VERSION     DACEPW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.282"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(4..1020)
                     /gene="tsaD"
                     /locus_tag="IW819_002686"
     CDS             complement(4..1020)
                     /gene="tsaD"
                     /locus_tag="IW819_002686"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1245764.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1017..1481)
                     /gene="rimI"
                     /locus_tag="IW819_002687"
     CDS             complement(1017..1481)
                     /gene="rimI"
                     /locus_tag="IW819_002687"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1245765.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(1488..2030)
                     /gene="rimI"
                     /locus_tag="IW819_002688"
     CDS             complement(1488..2030)
                     /gene="rimI"
                     /locus_tag="IW819_002688"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1245766.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2014..>2389)
                     /locus_tag="IW819_002689"
     CDS             complement(2014..>2389)
                     /locus_tag="IW819_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1245767.1"
                     /translation="RRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT          661 a          546 c          397 g          785 t
ORIGIN      
        1 tctttattca gataaaacca agcttggatc agcattcaac tgatagctag caaaatttct
       61 tttttgcatt tccacatgtg ctgctgctcc aatcattgca gcattatctc cacaaagtcg
      121 aagaggggga atcactagtt ctacctcagg gagtttagtt gccaaagcat cctgcaatcc
      181 ttcacgcaac ccttgattgg ccgctacacc tccagctata atcagttgct tgactggata
      241 attttgacaa gctcgaagtg ttttagtaat caatacatcg atcacacttg cttgaaagct
      301 agctgctaaa tcatttttgt ctagatcttc tccacgctgt tgtgcattat gcaccaaatt
      361 gataaatgca ctttttaatc cgctaaaact gaaatcataa ttatcttcat ggatcatcgc
      421 tcgcggaaaa tggtaattat cttttccctg atgagctagc tgatcgattt cttttccgct
      481 tgggtaagaa agacctaata ctcttccaac tttgtcataa gcttctcctg ctgcgtcatc
      541 tcttgtctcc ccaatgattt catatgatcc atcttcttgc atatagacta attctgtatg
      601 tcctccgcta acaagaagtg ccatcaatgg aaactggaat ggtttaacca gacgggctgc
      661 atagatatgc cctgccatat ggtttacagg aataagcggc aattggtgtg cccaagcaaa
      721 agccttagct gcagaaatac caatcagcaa cgagccgaca agtcctggtc cataagtgac
      781 agcgactgcc gataaatctt ctgccgacac accagcttct accagtgcat cttccaagca
      841 taaggtgatt tgttctacgt gatggcggct agctacttca ggtactacgc caccaaaacg
      901 tttatgactg tttatttgag aagcaacgat gttagataag atctctgttc catttctaac
      961 gaccgctaca ctcgtttcat cacaacttga ttcaatcgcc agaatcaatt cttcattcat
     1021 ttcatcgtct ccgtttttaa ctttctgctc ataatgatcg catcttccgt cggattgcga
     1081 taatatgatt tccgtttacc taatatacga aacttttctg attcataaag ttttcttgct
     1141 gcttcattag atagacgtac ttctaaaaaa acatgcagcg aatcttttat ttgttccgta
     1201 atcagtaatt gtaacaaatg ccgtgcatgc ccttttcctt gttctgaaac tgcaacagca
     1261 atatttgtga tctcgacttc atctattact ttcgagtatc caataaagcc acagatctgg
     1321 ttatcttcag ctaaaagcag ataatcagag tgaggctgtt ggatgtctgc caaaaactgc
     1381 attttcgtcc aaggtgaacc atgtggataa gcattttcgc taatcatcca tacgcattct
     1441 gccaagtacg cttcatcaaa atcttttttt ctgcaaatca actaatctca ctcattttct
     1501 aaaagtttta tcatatccaa cgcatcttct tttgtttcag tgtaatatct ttttttgatc
     1561 gttgttgctt gaaaccccaa tttccgataa agtctttgtg catcttgatt actcatacgc
     1621 acttctaatg acatcgtctc acagcgattc attatagcaa agttctcgat ttcgtcaata
     1681 agcagcgaac cgattcgttt acgctgatat gccggtcgaa cggcaatatt cgtgatatgg
     1741 caatctaaac cgaacgctct tgaaccgata aaaccaatca ttcttccatt atcaaagata
     1801 cctagatata gatgaggaag cggtgacttc aattctgata taaaggcact ttttgtccat
     1861 ggcaattcgc cattatagac ttctcgttcg acatccagca gctctttgat atcttctgta
     1921 gtcaattctc ttagtacgta acgacgatcg atttgttcga ctgttttttc tgtataaatc
     1981 attttatctt gaaaaaagct tctggcatac gctctaaatt ttttcaacat aactttcgtc
     2041 tccaggtgtg tgcgtctcca accatttttc ttctgcttct actcgtttta aatagcgagg
     2101 aagaaaagct tgtttctctg caactggttc aactaaagca cctaattgag ccacagttac
     2161 accacttgga atatcccatg ctggaactgt attaatcatc gcactaggca atatttcctt
     2221 tatttcagag aagaatttgt gacaatcact cccgacgaaa taaatagctt cattcagctt
     2281 ttgtagttgt gtgaacaaat cagaagcaga aatatgtaca tcttcaagta cagtttctaa
     2341 tctattttct ttccagcggt atgccccagc gtatacattt tttcttcga
//
LOCUS       DACEPW010000276         2373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.283, whole genome shotgun sequence.
ACCESSION   DACEPW010000276 DACEPW010000000
VERSION     DACEPW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.283"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(4..249)
                     /locus_tag="IW819_002690"
     CDS             complement(4..249)
                     /locus_tag="IW819_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245768.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="IW819_002691"
     CDS             complement(266..1117)
                     /locus_tag="IW819_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1245769.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="IW819_002692"
     CDS             1283..2287
                     /locus_tag="IW819_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1245770.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          754 a          402 c          478 g          739 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aac
//
LOCUS       DACEPW010000277         2365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.284, whole genome shotgun sequence.
ACCESSION   DACEPW010000277 DACEPW010000000
VERSION     DACEPW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.284"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1296
                     /locus_tag="IW819_002693"
     CDS             <1..1296
                     /locus_tag="IW819_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1245771.1"
                     /translation="SLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQ
                     FFSVVKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIF
                     TIAEAWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDI
                     IHTKKHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTS
                     LNPLHTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFID
                     EVINKRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAW
                     LRYLLAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSNIHCAVQEILENKEIFGL
                     DLYQVGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1299..>2365
                     /gene="uxuA"
                     /locus_tag="IW819_002694"
     CDS             1299..>2365
                     /gene="uxuA"
                     /locus_tag="IW819_002694"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1245772.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLQTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKG"
BASE COUNT          809 a          332 c          493 g          731 t
ORIGIN      
        1 agtttatatt gcaatccgaa gaatatagcg gactatcaaa gagtggttaa atattttgaa
       61 gatcctaaat tacagtttac aacgtttact attactgaaa aaggttacag tttaaaagat
      121 tctaaagggc aattcttttc ggtagtcaaa gctgatatca aatctggacc tagcaatcct
      181 caacatacga tgagtattgt aacatccttg ttatatagca gatttaatgc tggagagttg
      241 ccgattgcga tggtttctac tgataatttc tcacagaatg gtcaacgttt tcaagatagt
      301 atttttacga ttgcagaagc ttggcaaaaa gaaggatatg tttctgaaaa atttattgct
      361 tatttaaagg ataaagaaaa agtgactttt ccttggagta tgattgatcg aattacgcca
      421 aatccttcag agaatgtaaa ggaagatcta ttgatgatgg ggctcgagga tttagatatc
      481 attcacacaa aaaaacatac caatattgct gctttcacta atactgaggt agttcattat
      541 ttagttattg aagatagttt tccaaatggc cggcctaaat tggaaaaggc tggtgtgatt
      601 atgacagata gagagacggt tgataaagca gacatcatga aggttacgac aagcttgaat
      661 ccacttcata ctgctttggc agtaacaggt tgtctgctag gatatcaatc aatttcagca
      721 gagatgcagg atgatgattt ggttggttta atcaaaggaa taggttattt agaaggttta
      781 ccagtagttg aaaatccagg cattattgat ccaaaacaat ttattgatga agtaattaat
      841 aaacgtttgc ctaatcccta tattccagat acaccacaac gaattgcaac agatacttct
      901 caaaaagtag cgattcgcta tggtgaaaca attaaaaagt atgtcgaaaa agacggaaat
      961 gcagaaaatc taacatttat tcctatcgct attgctgctt ggttacgtta tttactggca
     1021 gtcgatgatc aaggggaaaa ttttgtgcca agtcctgatc cgttattggt agagttgcag
     1081 gataaattat cagtattgaa attaggtgaa caagatcctt caaatattca ttgtgcggta
     1141 caggaaattt tagaaaataa agaaattttt ggtttagatt tatatcaagt tggggtagga
     1201 aagaaaactg aaaaaatctt ttgcgaaatg ctagtaggtc ctcaagcagt tcgtaaaaca
     1261 atcaataaat atataacaga agttggagga aagtaactat ggaaatgact tttagatggt
     1321 atggggcaaa tgaagaaaaa atctcactac aagaaattcg tcagatccca gcaatgaaag
     1381 gaattgttgg aacattaatg gatataccag ccggagaggt ttggccgaga gaacgaatta
     1441 aatctttaaa agatgaagtc gaagccaatg gacttacctt gaaagttatt gagagtgtta
     1501 atgttcatga tgatatcaaa attggtttac aaacacgaga tcaatacatt gagaattata
     1561 aacagacgat tcgcaatttg gcagaatttg ggattgaggt tatctgttat aattttatgc
     1621 cagtatttga ttgggtaaaa tcggatttgg attatcgttt agatgatggg tcttctacgt
     1681 tagcctatat ttcaaaaaat atccctactg atccggagga aattattaga aaagtagaaa
     1741 attctactaa taattttgaa ttgccgggtt gggaacctca tcggttatca caaattaagc
     1801 aattattaga agcctataaa gaagttgatg aagagaaatt acgagagaac tttgcatatt
     1861 ttctcaaagc aattatcccg acatgtgaag aagttgggat taaaatggca gttcacccag
     1921 atgatccacc atattccatt ttcggattac ctcgtattgt taaaaatcgt gacgatttag
     1981 attggttgtg taatgtagtg gattctcctt ctaatggtat tacattatgt actggaagta
     2041 ttgcagaacg accagataat gatgtctatg aaattctatc tgaattttgt caacgggatc
     2101 ggattccttt tgcacatgtt cgtaatatca aatatttaag tgacaatgga ctcgattttt
     2161 acgaagcgcc gcataaatca gaatatggtt ctctagatat gtataagata ttaaaaacaa
     2221 tgtacgataa tggatttaat ggctatattc gtccagatca tggtcggatg atttggggag
     2281 aaaccggtag acctggttat ggattatatg accgagctct aggtgcagtt tatttaaatg
     2341 gtatttggga agcgcttgaa aaggg
//
LOCUS       DACEPW010000278         2361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.285, whole genome shotgun sequence.
ACCESSION   DACEPW010000278 DACEPW010000000
VERSION     DACEPW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.285"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(296..1297)
                     /gene="ccpA"
                     /locus_tag="IW819_002695"
     CDS             complement(296..1297)
                     /gene="ccpA"
                     /locus_tag="IW819_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1245773.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            1507..>2361
                     /locus_tag="IW819_002696"
     CDS             1507..>2361
                     /locus_tag="IW819_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1245774.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVI"
BASE COUNT          697 a          472 c          438 g          754 t
ORIGIN      
        1 aagcaactaa agaatcagat gagaaagatg attgatttaa tagaatacac agtaataagg
       61 gattgtgaca atagtattgt cacagtccct tatttttttg atggtggtga acaaaatgca
      121 gcatctatac acggattgtt ttatatacga taaatgtaat ttcaaccact ttctctcttt
      181 tttaagacac aaaaaaagga ttataacaaa attttgtcat aacccctttt ctcgaataaa
      241 cgttgttcca gaagttggcc acttttggaa caacctctaa atgattgatc ttttattatt
      301 tagttgatcc tttttcttcg attccataag gcaagataat cgttttttct tcgacttctt
      361 ctttattcat cattttcgtc aataggcgca tggataccgc tcctaaatcg tataatggtt
      421 gtgtgatact tgtcaaacga ggacgagcta cttctgtcag taaagaattg ttgcttgtga
      481 tgatttcgaa ttcttctggt acttttacgc cacggtctaa taaaccatcc aagataccaa
      541 tggctaattc atcgtctgaa acatagacag ctgttgcacc gctattatgg atccggtcaa
      601 ctagagacag accatcttta aagttgtatt gtgcctcaaa gattaatcct tcgctatatg
      661 gtagattgtt ttcgcttaat gcttctttat agccttttag acggttttga ccattgatag
      721 gatcaatcag cgcaccgctg acaaaagcaa tttttttatt gccgcttttt gctaatttat
      781 ttactgcatc ttttgttgct gatacataat caatgtttac gctacctact tgttcatctg
      841 gatcaatgga accagctagt acaacaggtg tttttgaacg tgaaaattct ccacggattt
      901 cgtctgtgat atggtgaccc ataaagatca cgccgtccac ttgcttagcc aataaattat
      961 tcaatacatt gacttctttt tgatcgttgc catctgagtt tgctaagatg atattgtatt
     1021 tgtacattgt tgctacatcg tcgataccac gtgctaaaga ggcaaagaac atattgctga
     1081 catcaggaat gattacccca acagttgttg ttttcttact tgccaagcca cgtgcgactg
     1141 catttggacg ataatccaag cgatcgataa cttccaagac tttttttctt gttgctggtt
     1201 tgacgtttgg attgccattt acgacacgtg aaacagtagc catcgaaaca tttgcctctc
     1261 tcgctacatc atagatagtg attgtttgct tttccatttg tgttctccta ttcatatttt
     1321 atatgttttc tgaaaatacg tcttacattt ctaaaatagg atatcaaata atctcaacaa
     1381 ttgcaaccgt tttatctctg ttttcatgaa aatttttact tttttcagtt ttaagaacgt
     1441 tggtctttta atcaatcagt ggtacaatta ttgaatacaa aattaattta gaaagtaggt
     1501 tgccaaatga ataacgtaaa aatcaatgaa ctacgtcaat ggatggcaca acaccaagta
     1561 gaattggctt atatcagcga ccctggccac atcgcttact tttccggata tgaaagtgat
     1621 cctcacgagc gtgtactagc tctttttatc cctcttgaac aagatccttt ccttttcact
     1681 cctgctctgg aagttgaaga tgctcaaaac agcagctggg cgtacgatgt acgtggctac
     1741 ttagacagcg aaaacccttg ggagatcatc ggaaaagagc ttcgcgcacg ctatgggcaa
     1801 cctggtaatg tagggattga aaagaatgct ctgactgtcg atcgcttcga tgctatctct
     1861 gatatcttga caggtgcaga ttcttacact gatctaactt ctattatcca gcgtctgcaa
     1921 ctgatcaaga cggtagaaga aaaagacaaa ctgatcgaag ctggtaaatg ggccgatgtt
     1981 gcctttgaaa ttggcttcaa aacagtaaaa gaaggcgtga ctgaacaagc aattatcgca
     2041 gaaatcgaat accaattaaa aaaacaaggt gtttcacaaa tgtctttcga caccttagtc
     2101 cttgcaggag caaatgctgc aagccctcat ggtacaccag gaagcacgaa ggtttcacca
     2161 aatgaactag tgttattcga tcttggcgtt gtttggaatg gctattgcag tgatgctact
     2221 cgtacagttg cttatcaaaa accaacagaa ttccaagaaa aaatctacaa catcactctt
     2281 gaagcacagc ttgccgcaca agaggcagtt cgcccaggtg tgacagcagg tgaacttgat
     2341 caaatcgcac gaaacgtaat c
//
LOCUS       DACEPW010000279         2358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.286, whole genome shotgun sequence.
ACCESSION   DACEPW010000279 DACEPW010000000
VERSION     DACEPW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.286"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..669)
                     /locus_tag="IW819_002697"
     CDS             complement(<1..669)
                     /locus_tag="IW819_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1062 family transposase"
                     /protein_id="HAQ1245775.1"
                     /translation="MTYTHLTSNKLAMIEAYYNNHQSVAKTAVLLNRSRQTIHKVYQF
                     FKTGHNALDYFNQYKKNKTRCGRRPIVLSDKQTEYIQKRVVQGWTPDVIVGRAEFSIS
                     CSMRTLYRMFKQGVFEVTHLPMKGKRKANGHKETRGKQSFRRSLRDRGNDYSKFNQEF
                     GHLEGDTIVGKKHKSAVITLVERLSKVIITLQPEGRRAIDIENRLNQWMQSVPKHLFK
                     SMTFR"
     gene            complement(751..2205)
                     /locus_tag="IW819_002698"
     CDS             complement(751..2205)
                     /locus_tag="IW819_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1245776.1"
                     /translation="MFSKEESSMGYKELGKDILANVGGSENVSSLAHCATRLRFNLKD
                     DSKADESAIRNLKGVVGVVNQGGQFQVIIGGDVKFVFAEIDKLGDFSASKESSSEANN
                     KGIVSKVLDTIAGIFVPIVPALTGAGMLKALLALLVMLEWMTPESQTYQFLNFIGDAA
                     FYFLPVLLASSAAKKFGCNQYIAITLGAILLHPTFTAMIAGARETGESLALFGLPVTL
                     ANYGSSVIPIILAIWFMSYIEPIIDKLMPNAVRIFMTPLLTLLIVAPITLIVIGPLGT
                     FLGNGLGSLIEFVNTYASWLVPLLVGAFTPLLVMTGMHYGLIPIGINMLATSGFDTVA
                     GPGMMVSNIAQGGAALAVAFRTKQLSVKQLAVSSGLSAFMGITEPALYGINMRFKKPL
                     IASMVGGGAAGLFIGIMGVGRYAQVAPGILALPSFFGEKGLSNFIYAAIGCAIAFVVG
                     FIASFILGIDEETEEVKEDTSEIPVNIASDNRRL"
BASE COUNT          764 a          486 c          372 g          736 t
ORIGIN      
        1 gcgaaaagtc attgatttga atagatgctt aggtactgat tgcatccatt ggttcaaacg
       61 attttcaata tctatagctc gtctgccttc tggctgaaga gtgatgatta ctttagataa
      121 tcgctcaacg agggtaataa cagcactttt gtgcttttta cccacaatag tatccccttc
      181 aaggtggcca aattcttgat tgaatttaga ataatcattt cctctgtcac gaagtgatcg
      241 acgaaaagat tgttttcccc gagtttcttt gtgtccgttg gctttacgtt ttcctttcat
      301 aggtaggtga gtcacttcaa atacgccttg tttaaacata cgataaagtg tacgcataga
      361 acaagaaata gaaaactctg cacggccaac aatcacatca ggtgtccaac cttgaacaac
      421 cctcttttga atatattctg tttgtttatc tgataagaca ataggacgtc tgccacagcg
      481 agttttattc ttcttgtatt gattgaaata atctagagca ttgtgccctg ttttgaaaaa
      541 ttggtaaact ttatgaatcg tttgtctaga tctattcaat agtactgcgg ttttggctac
      601 agattgatga ttattataat acgcctctat cattgcaagt ttatttgatg taagatgggt
      661 ataggtcatt tgtcttcact ccttataatt cttttgcggg aactatcttg agtgtagcat
      721 aaatgacttt tttatttgtc tagcttaatt ttacaatcgg cgattgtcac ttgcaatatt
      781 tactggaatt tcgctcgtat cttcttttac ttcctcagtc tcttcatcaa tacctaaaat
      841 gaatgaagca ataaaaccga caacaaatgc aatcgcacaa ccaatcgctg cgtaaataaa
      901 atttgatagt cctttttccc caaagaaact tggtaacgca agaatgccag gagcaacttg
      961 agcatatcga ccgacaccca tgattccaat aaatagtccc gctgctccac cacctaccat
     1021 tgaagcaatt aacggctttt tgaatcgcat attaattccg tataaagctg gttcagtaat
     1081 tcccataaaa gcagacagac ctgaagaaac agctaattgt ttgacagaaa gttgttttgt
     1141 tctaaaggca actgctaagg cagcaccacc ctgagcaata ttggaaacca tcatacctgg
     1201 accagcaaca gtatcaaatc cagatgttgc taacatatta ataccgattg gaattaaacc
     1261 gtaatgcata cctgtcatca caagtaatgg agtaaatgca cccacaagta atggaaccaa
     1321 ccaacttgca tacgtattaa caaattcaat aagtgaccct aacccattac ctaagaatgt
     1381 acccaaagga ccaatcacaa ttaaggtaat tggtgcaaca atcaacaatg ttaacagagg
     1441 cgtcataaat attcgaacag cattgggcat caatttatcg ataatcggct cgatatatga
     1501 cataaaccaa atagctaaaa taatcggaat aacagaagaa ccatagttag ctaacgtcac
     1561 aggtaatccg aataaagcta aggactcacc tgtttctcta gcacccgcaa tcattgctgt
     1621 aaaagtcgga tgtaacaaaa ttgctcctaa ggtaattgca atgtactgat tacatccgaa
     1681 tttttttgca gcagaactag ctaataagac cggtaaaaaa taaaaggctg catcaccaat
     1741 gaaatttaaa aattgatagg tttgcgattc tggtgtcatc cattccaaca tgactaatag
     1801 agctaataat gcttttaaca tccctgcacc tgttaatgcc ggaacaattg gaacaaaaat
     1861 acccgcaatt gtatctaaaa cttttgatac aatcccttta ttgtttgctt ctgaactact
     1921 ttctttagaa gcggaaaaat ctcctagttt atcgatttcg gcaaatacaa attttacatc
     1981 tccaccaata atgacttgaa attgaccacc ctgattaact acacctacaa cacctttaag
     2041 atttctaata gctgattcat ctgctttact gtcatctttt aaattaaatc gaagtcgtgt
     2101 tgcacaatgc gctaaactgc ttacattttc agaaccacca acatttgcta atatgtcttt
     2161 tcctaattct ttatacccca tgctactttc ctccttagag aacattttct cttttgataa
     2221 tttgaatata gcgtgctatt aatagataaa caatacattt gaaaccgttt tacacagcgt
     2281 tgcgaaattc attcagttta cgattatttt ttcgcaacac tgttccaaaa aaataaaatc
     2341 cacttactaa ctaatcgt
//
LOCUS       DACEPW010000280         2273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.287, whole genome shotgun sequence.
ACCESSION   DACEPW010000280 DACEPW010000000
VERSION     DACEPW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.287"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..209)
                     /locus_tag="IW819_002699"
     CDS             complement(<1..209)
                     /locus_tag="IW819_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1245777.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(308..622)
                     /locus_tag="IW819_002700"
     CDS             complement(308..622)
                     /locus_tag="IW819_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245778.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(690..1184)
                     /locus_tag="IW819_002701"
     CDS             complement(690..1184)
                     /locus_tag="IW819_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245779.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1177..1491)
                     /locus_tag="IW819_002702"
     CDS             complement(1177..1491)
                     /locus_tag="IW819_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245780.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1707..>2273)
                     /locus_tag="IW819_002703"
     CDS             complement(1707..>2273)
                     /locus_tag="IW819_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAQ1245781.1"
                     /translation="KRYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTG
                     ELNVPFVELDSIAQKQFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHT
                     DVNPQTLRSAKSKTISIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKM
                     YRAKKDYEFTQQMIENQQKRQQNFEMEF"
BASE COUNT          680 a          425 c          306 g          862 t
ORIGIN      
        1 aaatcagata cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac
       61 tcccattttc tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta
      121 gtaatttcta tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct
      181 ttgaagtttg ttggagtaat agtatccaaa atgaacacct ctcttctcta tacctctatt
      241 atgaagtgcc cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat
      301 taagctttca ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa
      361 cttcttgtgg ttgataatag tctggatcta tttcaaatgt ttgtgagaat aattcattga
      421 atttttctac tgaaataatg attttaccat tcttctctcg actgttttct attatttctg
      481 cttctaattc aaataaatct agtgacgaac taaaatcata aatcatgtca tattgaataa
      541 aaaaatcaag aaaaagttgc tcaaactctt ccttatttat cactaatgtt tcaaacggaa
      601 caactttatt ttcctcagcc attctcccag ttccattttc aaaaatcaca aaactgatat
      661 taccttaaaa actactaaca aaataaaact tataaagtaa atggcaaccc aactatgtaa
      721 ccattctctc tacgtactac gatttggtag tcctgccttt caatcggtaa ttcataatag
      781 taatttactg tttcttcttt gccactttca atatactctg gattatccaa tatcatatca
      841 tactcataaa gttcttcacc atccttattt tcaatcgacc attgttgtgg acgtatcgga
      901 ttttctgtat catttataag tgtataggtc acttgtaata cacgatcatg ttgtgcaaat
      961 tcatttcttt tttcggtcac ttcaatattt ttaaatgtta gttcaacccc atctgcctga
     1021 taagtttcac ctatttttaa atcttttgtt acaattttta ttggcttttc ttctggaaaa
     1081 tgtctggctt taattgttag ataacttcca aaaagaatca atcctagtag cactaaaaga
     1141 attcctttaa cgagccactg aaacgggaag taaactttaa acatcttttt tctcctccgt
     1201 tttgtatttg ttttttagag aatacgataa cttttgatca atgtcatcca gcttttcaat
     1261 tacttcatta cttatcattg attctagttc tgaaattttt tgttcaaatt taatgctttc
     1321 aggatcatta aatttattat ttgatggact agctaacact ggtaaatttg aaaccataac
     1381 acgaaacata tttgcctttg aaccaaaaat tcctttgttt tggttgtaaa aattgttgaa
     1441 tatttcttta gtttcttctg aagccgagac tttaaaatat ggatagttca tttttttctt
     1501 ccacctttct ttttttggga gggagtctgt agggagcttg ctccctcaga gatttacccg
     1561 ccatctgact tgccagaaag tagcctagcg tgagcggggc ggattgactg gcaagtagat
     1621 gggtagctgg cgtatgtcct aaaaactgct tctttccaag caactcttca taggcaaaaa
     1681 actggaaagt ccactaacaa gcccattcag aattccattt caaaattttg ttgccttttt
     1741 tgctgatttt caatcatttg ttgtgtgaat tcataatcct ttttagcacg atacattttt
     1801 acatcgtcat tcattatgcg actgatttga tagtgaatct gtttagcaga aggtgtatga
     1861 gaacaatttt caaaaccctt tgccggtaga aataactctt tctgatcagt ctttttaata
     1921 gaaattgttt tacttttcgc actacgtaaa gtttgaggat tcacatccgt atgaagttta
     1981 atcgtgtatt cttgatcatc ttctaaagac attgtattta caaaatgctt gtctacctct
     2041 cgcaagaagt catcatattt aatgaatttt tcatcgcctt cacggtcgct aaattgcttt
     2101 tgtgcgatgg aatctaattc cacaaacggt acatttaact caccagtttt gtaatttact
     2161 ccactaatta gcgacataac ttgctctctt tccatattaa aaaaattttt tatatgtgat
     2221 tcttctaaat tcttttcaag ttgttcagaa acagattcaa atgaatatct ttt
//
LOCUS       DACEPW010000281         2269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.288, whole genome shotgun sequence.
ACCESSION   DACEPW010000281 DACEPW010000000
VERSION     DACEPW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.288"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..223)
                     /locus_tag="IW819_002704"
     CDS             complement(<1..223)
                     /locus_tag="IW819_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1245782.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRK"
     gene            complement(367..1098)
                     /locus_tag="IW819_002705"
     CDS             complement(367..1098)
                     /locus_tag="IW819_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1245783.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(1167..2108)
                     /locus_tag="IW819_002706"
     CDS             complement(1167..2108)
                     /locus_tag="IW819_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1245784.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
BASE COUNT          629 a          511 c          377 g          752 t
ORIGIN      
        1 ttttacgttt ttctaagttt tcaagatagg cataccgctc ttcgatttca cgaatctgga
       61 cttcatcaag acttccagtc atttcttttc ggtaacgcgc gataaaagga acagtgtttc
      121 cttcatttaa caaattaagt acagtagtca actgcttagg acgatagtct gaaagttctt
      181 tttgtacaag ctgaatgatc gtctggttca aattttccgt cattaaaatg cctcttttct
      241 gctaaaaatt gcttctttat tctatcatac tttttatcct gggttaaact actgaaagaa
      301 acaatattct ctataaaaat aaaaagagcc gcaacaaagt cacagctcct tcattctttc
      361 cattagtcag attccatgcc aaggtccttc gttgttctga tattcaatat cttgaagaat
      421 accttccttg tcaaaaacaa ccccatgcaa tgaccctccg aatacagccc caccgtccat
      481 tccgatttta tgatcctgaa tccagaggtc tgttgtctgc atatctccat ggagcattgg
      541 tgtaatcgta tgtccaaata caattgtctt acctgtattg tttttccctt catgaaatgg
      601 ttcccttatc caaataaagt ctcttagact tgtcttacgc cagtctttct ttgtcaaatc
      661 cacaccggca tggacaaaaa gataattttc ccattcataa tacaaggggc gctggactaa
      721 aaatttaatc agttctgaat atctcgattg gatcatcatc gagatttcag ttggggagta
      781 ttcctccgat gcccctgggt gaatcaggct ttcaaacgtt tcttttccac catttctcat
      841 ataaggcgca taccagtcct ccggtgattg cagaaattgc aaaaaatatt cttcatggtt
      901 tccccggagg taaatggctc ctgtttcttc gactaattgc tttcctaata aaaaacattc
      961 ttttgtacga ggacctcgat cattgagatc ccctaataaa acaagctgat ggatctttgg
     1021 atcataatag tgaataagtt tttcaaaaag ctcatgtttg ccatggacat ccccaatagc
     1081 ataaatcaat gatttcatgt ggatcaccat cctcaacgct agtatagcgc atattggaaa
     1141 tggtcgacaa tcaaaactat tgttttctat ttaattaaag ctgtaaaccg cagttctgct
     1201 tcttctggag agattccatg gtcttgaaca tagcgattgc gcggatgttt cgcacactct
     1261 atgctacatg ctcctaaatg tttttcttca ttttcaatgg aagcaagaat ctgacgattg
     1321 cactcaggat tgctacaatt gatatatcgt tcacatggtg ttccgtcaaa ccagtcgcga
     1381 ccgacgattt gtttatctac ttggttgata tcgacagcga tccgttcatc aaacacatac
     1441 atcttgccgt cccataattc tccttggact tcaggatcct taccataagt agcaataccg
     1501 ccatgcagct gtccgacatc tttgaatcct tcacggacaa gccaaccaga aaacttctcg
     1561 caacgaatgc caccagtaca gtaggtcact acccgttttt ccataaattc ttctttgtgc
     1621 tcgcggatcc agtctggaag ctctcgaaaa ctgcgtatat ctgggcgtac agcccctcga
     1681 aaatgtccca agtcatattc ataatcattc cgtgcatcaa tcaccactgt ctgttcatct
     1741 aaaatagctt ctttaaattc ttgcggtgaa agataggcac ctgtcacttg gtttggatca
     1801 atgtcctctt ctagctttaa acttacaagc tctggacggt atcttacaaa catcttctta
     1861 aaggcatgtc cttcgcttgg atcgatcttg aacatcgtat cagcaaatcg aggatcagct
     1921 ttcattgctt tcatataggc agtcgtgttt tcaacggttc cagacacagt accatttatt
     1981 ccttcttcag ctactagaat acgaccttta agatccaaag atttgcaaaa ggctaaatga
     2041 tttttggcaa attcctctgg ttcttcgatt tttgtataat tgtaataaag cagtacttga
     2101 taatccatgt atttcttcct tccttctttt cgttcctaaa cagtataaag aaggcgttcg
     2161 catttgacga tttatttgct tgtgtataat tacgcatgga aagttttttg tttacataat
     2221 attattattt gaattaaaaa gacttcgctt tcctgtttcg cacagaaaa
//
LOCUS       DACEPW010000282         2255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.289, whole genome shotgun sequence.
ACCESSION   DACEPW010000282 DACEPW010000000
VERSION     DACEPW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.289"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..448)
                     /locus_tag="IW819_002707"
     CDS             complement(17..448)
                     /locus_tag="IW819_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1245785.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(512..1348)
                     /locus_tag="IW819_002708"
     CDS             complement(512..1348)
                     /locus_tag="IW819_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1245786.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(1538..1840)
                     /locus_tag="IW819_002709"
     CDS             complement(1538..1840)
                     /locus_tag="IW819_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1245787.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(1866..>2255)
                     /gene="ruvX"
                     /locus_tag="IW819_002710"
     CDS             complement(1866..>2255)
                     /gene="ruvX"
                     /locus_tag="IW819_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1245788.1"
                     /translation="VAVSDPFGWTAQGVEIIRINEAEEEFGFDRLKELVAQYEVDRFV
                     VGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQAERMLVEQANTSRA
                     KRKKVIDKLAAVMILQNYLDGSGKQLF"
BASE COUNT          660 a          509 c          353 g          733 t
ORIGIN      
        1 attttttgat aaaaatttac aggattttca aaaatctttt ttgcatttta tcggaatgat
       61 aaccgatgcg ttcataaaat cggtgtgctt ctactctgta ttctgcagaa ttaagtctta
      121 ttgctgatat tcctcgtctt ttcgcttctt ctacgccagt catcaaggcc tttccgattc
      181 ctcttttttc agcatgttta gacacagcaa gtgccataat attgaacatc ggatcgctga
      241 aaatcgattc ataggactcc gcttgtacat agccagctgc ttttttagaa gtaggatctt
      301 catagaccaa gaaaaaattt ttttctggct ccttcaacag tttcttcatt tgtctcaccg
      361 taacggttaa tggtacatca tatcctaatt gttccgcatt gatttctcgt agtgcttctg
      421 catctcctaa acgcatttcc cgcaacatcc aaatcaccag ccaattttta tttttttaaa
      481 tgaatttttt tatcacagta tactcctttt ttcacttaaa aaaagacttt tcagtgattt
      541 tgacttgctt gaatccagat ccttctaatg tttgcaagat gacgaaagca ttgtaaagat
      601 tgatcaaaag aaccacgatg acaatcgctt gaaaaataaa ggtcactttt tttggagaca
      661 aaaccctgct aaatcttgct ccccacaaac ctccaataac agcagcgggg ataacataaa
      721 acaacataga taaatcaaag cgcataaagc tacttgttaa agcaatcgtt accaattttg
      781 ccagttgaga aaagaaaatc gtagatagag aatatagtgt tgcttctttt attggcaatg
      841 caaacatcag catcaacaaa gaaacattga ctggtccacc accaatacct aaaaaactag
      901 ctaaaaagcc cagaaccccc ccacatataa ggtaccaagt ccatgacgtc aaatgaaatt
      961 ttggctgatg atgcttggtg tagaagaacg caaaaaccaa ggtcaatacc gtcaaaaaaa
     1021 tttggatcag ctgcacatgc ttctcgtttt caaataactg taaaaaaact tcgaatacaa
     1081 tgttgccgac tactcctcca acaacagctc cactagatac ccaaccgaca atctgccaat
     1141 tcagtgattt cccgcttgat acttgtcgca cagtcgaaac aatggacatc gtgaataccg
     1201 caactgttga gtaaaaagaa atccctgcaa cagaatgggc gccgataaga tcaagaatcg
     1261 gtttgatcaa cacgcctcca cccatcccgg aaatagcgcc aactgtgttc gccagaacga
     1321 tcaccacaaa ataaatcaga ccaatcatct tgtatttcct ttctcttgtt tatatctttt
     1381 agcgattgtt actcttttct tttattatac agtaaaagaa acaaacgagg cttcgaaagc
     1441 ttttctactt tttaaggaaa tttagtgcta aacgaaaaaa ccgggctcta cttttcagag
     1501 agtccagctt ttcttacacc ttatcgattc ttttgttcta ttcttcttcc gccatgaatg
     1561 tattgaatac ttcttcgatc atatcccatt ctgcgtctgt ttcgatttgt tctaactcgc
     1621 cttctgttcc atcttcgttt tcgatgtatg cataagcttg taattctaca tcttcatctt
     1681 ctgaaacacc agctggatat aaaagaacat agttacgtcc aaattcttct tgtccgtcaa
     1741 ctgttagcaa gatttcgtat aatgtttcat ttccttggtc atccaccaat gtgatgtgtt
     1801 cgtgtccttc gtgttcgtgg gaatggttat gttctgtcat aagattacct cttttctttt
     1861 tattattaaa ataattgctt acctgagccg tctaaataat tttgtaaaat catcacagct
     1921 gctaatttat cgatgacttt ttttcgtttc gctcttgaag tatttgcctg ctctactagc
     1981 atacgttctg cctgaacagt ggttagtcgt tcatcttgat aatcaaccgg caaggagaat
     2041 agttctttga tcttctctcc atatgacatg gaagcttctg cccttggtcc gatggaattg
     2101 ttcatattct taggtaatcc aactacaaaa cggtctactt cgtactgcgc cactaattcc
     2161 ttcaaccgat caaaaccaaa ctcttcttct gcctcattga ttcgaatgat ttctactcct
     2221 tgagctgtcc agccaaaggg atcgctcaca gcaac
//
LOCUS       DACEPW010000283         2253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.290, whole genome shotgun sequence.
ACCESSION   DACEPW010000283 DACEPW010000000
VERSION     DACEPW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.290"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(243..812)
                     /locus_tag="IW819_002711"
     CDS             complement(243..812)
                     /locus_tag="IW819_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1245789.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(886..2175)
                     /locus_tag="IW819_002712"
     CDS             complement(886..2175)
                     /locus_tag="IW819_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245790.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
BASE COUNT          624 a          460 c          392 g          777 t
ORIGIN      
        1 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
       61 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
      121 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
      181 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
      241 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
      301 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
      361 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
      421 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
      481 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
      541 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
      601 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
      661 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
      721 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
      781 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
      841 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
      901 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
      961 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcggtgatca ctagatctgc
     1021 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1081 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     1141 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     1201 taaaagcatc ccccactcta ctgcggggtg tgcaaatgct tgttggatcg ctagccgaaa
     1261 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     1321 ttcttctatc agccggtcat ggataccaat tacgtaacga agccattctt ggtaaagtac
     1381 ttttgtcagc tttgatttgt tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     1441 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     1501 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     1561 tattggcaaa atctgctctt ctctaaatag caattcagga aacaattcaa agaatagtgt
     1621 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     1681 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     1741 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     1801 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     1861 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     1921 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     1981 aacggttttc ttatgaagaa tcagctggat gggacagtcg ctttccgcca aacgtttcag
     2041 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     2101 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     2161 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     2221 gggttcactc ctaaaaagat aaaaaactcc ctt
//
LOCUS       DACEPW010000284         2230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.291, whole genome shotgun sequence.
ACCESSION   DACEPW010000284 DACEPW010000000
VERSION     DACEPW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.291"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..321
                     /locus_tag="IW819_002713"
     CDS             <1..321
                     /locus_tag="IW819_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1245791.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            735..1553
                     /locus_tag="IW819_002714"
     CDS             735..1553
                     /locus_tag="IW819_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1245792.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1626..1985)
                     /locus_tag="IW819_002715"
     CDS             complement(1626..1985)
                     /locus_tag="IW819_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1245793.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          751 a          323 c          415 g          741 t
ORIGIN      
        1 tataaagcaa tcattcctga gaacttagat gaggttaaac tttatttatg ggaagataac
       61 ggagaaattg tcggttttag tggtgtaagt gcagaagaat tagttatgct atttcttgat
      121 ccagatttta ttggtaagcg atatggtagt caaatattaa ttgagctgat tgaaactgaa
      181 gatataaaaa gaattgatgt taatagtcaa aataaatatg caaaacattt ttatttaagc
      241 catggatttg aaattgaatc tgaagaagac ttagatggtt ttggtaaacc ataccctata
      301 acgcatttag taaaaaaata attcatgaat ggtttattac ctgattgaac atgaataaaa
      361 gtaatatttt tattttgatg gcctatttct aggttatgaa ttatccagat caaatttgtg
      421 tattacattc agtaataagg tgcttatctt aggatcaact tatgatacta gctgaccaag
      481 gatgtaccag ttatagaaaa tcagttaaag taaaaatgat aaaaatctta tgtcaacact
      541 gatttaggct gacctgtggg ttaggagaaa gacaggtcgg tagcttttaa tacaaaaaat
      601 ggcttgtatg tatctagata gttaactgaa attagaaatg ttcttatttt taacggagta
      661 agaaaaatag gctgctttag gtctgaatac tttttcaata taatgaagat atcaaaggcg
      721 acaggagtgt ttttatgaat aagaatcctc ataatctaaa gttggtagca gtagatatgg
      781 atggaacatt tgctcgtact gattacacat acgatatccc aaggttcaag gctgttttat
      841 cgcgaatgaa agctgttgat tgtcaatttg ttgttgcaag tggaaatcaa tactaccaat
      901 tgagatcgtt atttccaaat tattatgatg aattaagttt tgttgctgaa aatggtgctt
      961 ttgttaaaga taaaacagag gtactatttt cagccgatat gcctagaaaa gcgatcgagg
     1021 tggtccttga tttatgtaaa gactatcctg aagtaaaaaa tgtgatgtgt ggtctgaata
     1081 gtgcctactg tgaaagagga aaggtgagtc aagcattttt tgatctaacc aatatctatt
     1141 accatagatt agaatgggta gctgattttg cagaagttca agataaaatt ttaaaatttg
     1201 ctccgactat gcctgttgaa aaaaccgaat tttacgttga attgttccgt gaaaaattag
     1261 ccgggctatt agtacctacc agtagtggtc atggttctgt agacttgatt ctttctgagt
     1321 gccataaaac tttcggcttg aaaatgttag ttgagcgctt ggggattaat cctgaccagt
     1381 gcgttgcttt tggagatggg ggcaatgaca ttgagatgtt ggagtattgt ggtctgagtt
     1441 atgaaatgga taatgcaacg gaagctgtta agcaagttgc taagcatcaa tgtccttcaa
     1501 atgacgaaga cggtgtttta gtgacgctcg atcgattgtt ccctaacacg taaacactag
     1561 gtaattcatc aaaaaacctc gatataagaa atattcgagg ttttaaaact atatttagaa
     1621 cagatttacg aagcgtctga tgagtcaaaa tagacttgga cattgctatc taaaatacca
     1681 ctttcattct ttaaacgaat tgtttgattt gatggagtta cctctaatac taaatgccca
     1741 ttaataaagg tagtatcgta ttcagatgtt ttaactgttg cttcttcgca aggtattgtt
     1801 aaatcatatg ctggaatata atgttgtgtg gaaactaata aaaaatgatc accaatcata
     1861 cagcatccct ctgctgaaga aaattgatta gagccatcat ttaaagttaa caagaatcca
     1921 tcctttaaac gaggtacttg cttaaatact tctggttgta cttctaaaaa taaattatcg
     1981 ctcattttca cgaccaccat cgacttccac agttgaaatt gacattttat ttaacacacc
     2041 tattccccct taactataac tcaattctat ataaaaaata ttctattctc aaataattga
     2101 acttttcaaa tcgccaattt acttcggctt tatcagagct cattcatatc aagagcgatt
     2161 aacgctgtaa aaattaaacc gaagatctga taaattttct ctattttcta caatatttaa
     2221 aacgcttcct
//
LOCUS       DACEPW010000285         2228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.292, whole genome shotgun sequence.
ACCESSION   DACEPW010000285 DACEPW010000000
VERSION     DACEPW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.292"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            164..1204
                     /locus_tag="IW819_002716"
     CDS             164..1204
                     /locus_tag="IW819_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1245794.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSLDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1256..>2228)
                     /locus_tag="IW819_002717"
     CDS             complement(1256..>2228)
                     /locus_tag="IW819_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1245795.1"
                     /translation="YIGNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPATSGS
                     LTGSSILDGLDGSFHFSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSG
                     NSLLSSEPNHNLFGIKGSYNGSSVTFNTHEQDSSGQSYQIRAQFRKYPSYKESLEDYA
                     DLIKNGLTGNPDFYKPTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKY
                     DEPKKEAATTEEANAEILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVA
                     SIWDLWNNFTARQIPETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT          668 a          399 c          484 g          677 t
ORIGIN      
        1 ggaaaaagag gttgtgacaa tacttttgtc acaacctttt ttcgcggtct tttattagaa
       61 tatagtaaat tttttctcgt tactcacgga ataagctgtt aagaaaacgg tttctatgta
      121 tactggaatc agtgcaaatg aaagaaaaga gggtgcaagg aacatggaaa tcaatgaaca
      181 aaattttggc gaaaactatc acttgattac tatcacgaat caaaatggga catctttgtc
      241 tgtcagtgat cttggggcac gggttgttca ttttcaaaca ataatcgata atgaaaaacg
      301 agagttgatc ttaggttttg atgaagcaaa agaatatcta gaaaaagacc cttatatcgg
      361 tgcttcaatc ggacgtatgg ctggacggat cacgaatggc acattcaaat tagatgatga
      421 aacgttcgaa gtaaaaacag acccggacac tggtcattgt ttacatggtg gtgcacctag
      481 ctttgaagcc aagcagtggc attatacagt catcaatggt gaaaacgaag catcggttat
      541 tttctatcag actagcccag acatggaaaa cggatttcct ggaactttag atgtagaagt
      601 acggtatacg ctgacgaacg atgatacatg gcgagtgacg gtccaaggga tcagtgaccg
      661 caaaacagta ttcaatccta caaatcatgt ctatttcaat ttaaccggtg atccagcaca
      721 gtcaatcgac gaacatatac tttggttgaa tagccgatta tttgctacgc tcaataaaga
      781 tacaacgcct acaggagaag tagtttcagt aagtggcaca gcatttgact tccaacgtcc
      841 taaaaaatta ggtgaggtct ttgccagtga ttttcgccaa tcaatgatag tgggcggtat
      901 cgatcatcca tttttgttga aaaaacaatc tggtttacta aatgctgcgg aattgacaag
      961 cctagatgaa aaagtatcga tccaagtaaa aacagatgct tcaggaatcg tgatttttac
     1021 agcaaatttt ggagaagatg gaccggaaat gcgcgggaat aaattagtaa atcatggcgg
     1081 tatcacattt gagactcaag aactgccagg agcggagcga cttgaaagtt ttggagatgt
     1141 cactctggaa cctaatcaag tacgcgagtt tgtaacggaa tacaaaataa atataaagaa
     1201 atagaataaa ttaaaaaggg agtgtgacag tgtcacatcc cctttttaat ttattctatt
     1261 tctttaaagg cagatcaagc cagaatgatt tgtctaaact taacaaggtt agtacaggaa
     1321 cagttgcagc tttaactgta cgagtagttg tttctggtat ctgtcttgcc gtaaaattgt
     1381 tccacaaatc ccagattgaa gcgacacgga agatggaaga agcgttgttg acgcgtttta
     1441 tcgttgaaaa ttctaatcct gtgattggca cgccatctgt tgtccatttg attggcacat
     1501 cgaatcgctt agacatattg aggatttcgg cgtttgcttc ttcagttgtt gctgcttctt
     1561 ttttcggttc atcatatttt gtcaggtcgt acgcttcgat gatcgcgttc aatttttgtg
     1621 aatattggcg atctgtagca taacgtcctt ccaaatattt ggttgcttct ttgtagtctt
     1681 ttgtttccga cttccaagtt ggtttgtaga aatcaggatt gccagtcagt ccatttttga
     1741 tcaagtcggc atagtcttcc aaactttcct tgtaagaagg atatttacgg aattgtgcgc
     1801 ggatttggta gctttgtccg gaactatctt gttcatgtgt attgaaggtc acgctgcttc
     1861 cattatatga tcctttgata ccaaaaaggt tatgatttgg ttcgcttgat aataagctgt
     1921 ttccagatcc actttcaagg atagcttgag ccatcatgac tgatgcgtag attccttctt
     1981 tttgcccgat ttcctgtgca tcatctgcaa tctttttcaa gaactgatca gttgtttggt
     2041 tttgactgaa atggaacgag ccatctaatc catctaaaat agatgaacca gttagcgagc
     2101 ctgaagtagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc gtgaaatctg
     2161 aaatagaagt tactcgaact ttatctcctg gttggggcgc atggataaat tgtccgttcc
     2221 caatatag
//
LOCUS       DACEPW010000286         2221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.293, whole genome shotgun sequence.
ACCESSION   DACEPW010000286 DACEPW010000000
VERSION     DACEPW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.293"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>2221
                     /gene="esp"
                     /locus_tag="IW819_002718"
     CDS             <1..>2221
                     /gene="esp"
                     /locus_tag="IW819_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1245796.1"
                     /translation="GVGLVLGTTELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQ
                     ENPGYTKNYNFSDLQFSPQELTGDTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERL
                     AKYVEKIQVDPKKGIGSSRRTFVRINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQT
                     APNGVITFEKSLDEIFKEIGIDNLKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDS
                     DDFYNSLDVSENNPDQFKHGSVSAKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNF
                     SYSSSAKGTPWYANYKIDERLVPYVAGIQMHMVQADKVTYDVSFESGTKVADLAIERR
                     EGHENYGIGSITNNDLTKLIDFANASPRPVVIRYVLQLTKPLDEILEDMKATAQVEEN
                     KPFGEDFIFDSWLSDTNKKLIQNTYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPE
                     LEEVYDVDTTVKGKVFIHELAGTGHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEF
                     EFTGVDSSKLIAKDELKIQIVSPGFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKES
                     IRNNKNLPEDAEYSWKTEPDTSNVTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMEND
                     ATYVPITTTPETTVQSGKPTFDKPDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFT
                     PAKGVGESEPITGTIPIKIVYQDGSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTA
                     "
BASE COUNT          801 a          362 c          451 g          607 t
ORIGIN      
        1 ttggtgttgg tcttgtcttg ggaacaactg aactagttaa agcacaagat gaaatttctc
       61 caagcagccc acttgaaaca gcgataagct ccgtacaagt aggtgacaaa gtagcttcgg
      121 ggaatacttt tcaggagaat cctggttata cgaaaaatta taacttttcg gatttacaat
      181 ttagtcctca agaactaact ggagacacac taaaaggcaa tacaattgga tttgaagttt
      241 acggaaaaca taatattgca gcatcaacta aaaactggga aatccgtctt caattagatg
      301 aacgattagc taaatatgta gaaaaaattc aggtcgatcc aaagaaagga attggatctt
      361 ctagacggac ctttgtaaga attaatgatt cgctgggtag acctacaaat atttggaagg
      421 tcaattatat tcgtgcaagt gatggattat ttgctggggc agaaacaacc gatacgcaaa
      481 ccgctcctaa tggggtaatt acgtttgaaa agagcctaga tgaaattttt aaagagatag
      541 gcatagacaa tctcaagacc gatcgtttaa tgtatcgaat ctacttggta agtcatcaag
      601 acgatgataa aattgtaccg ggtattgata gtacgggcta ctttttaaca gattcagatg
      661 atttctataa tagcttagat gtatctgaaa ataatcctga ccagtttaaa catggatcag
      721 taagtgcaaa atatgagaaa cctaatactc aaacaaaaga tggttcagga tccacaggtg
      781 ctaatggagc tataatctta gatcataagt taacgaaaaa ttataatttc tcatattcat
      841 cttccgcaaa gggcacgcct tggtatgcta attacaaaat tgatgaacga ttagttccat
      901 atgtagcggg tatacagatg cacatggttc aggctgacaa agtaacatat gatgtttcgt
      961 ttgaatccgg aacaaaagta gctgatttag caatcgagcg acgggaaggt catgaaaatt
     1021 atggcatagg ttcaataact aataatgatt taactaaact tatcgatttt gctaatgcaa
     1081 gtccacgtcc agtcgttatt cgatatgttt tacaattgac taagccatta gatgaaatct
     1141 tagaagatat gaaagcaaca gcacaagttg aagaaaataa accatttggt gaagatttca
     1201 tctttgattc ttggttgtcg gatacgaata aaaaattaat ccagaacact tatggaacag
     1261 gttattatta tttgcaagat attgatggtg atggaaaccc tgacgataaa gaagagagcg
     1321 gagacacgaa tccatatatc gggaaacctg aattagaaga agtatatgat gttgacacaa
     1381 cagttaaggg gaaagtattc atccacgagt tagcgggaac aggtcacaaa gcccaacttg
     1441 ttgataaaga aggtactgta ttagcagaaa aaactatcgc tccaaatgaa aaagatgggg
     1501 ctccaatttc agatactgta gaatttgaat ttacgggtgt agattcaagt aaattaatcg
     1561 cgaaagatga attaaaaatc caaatcgttt ctccaggttt tgataaacca gaagaaggtt
     1621 caaccgttat taaggaatca ccaaaagcgg ttgataaaca aaccgtggta gttggattta
     1681 aaccagatgc taaagaatca attcggaata ataaaaactt acctgaagat gcagagtatt
     1741 catggaaaac agagcctgat acttctaacg ttactgatag tacgaaaggt attgtaactg
     1801 ttaagatcgg aaatcgaact ttcgacgtgg atgtagagtt tgctgtaaaa gcttctcaag
     1861 ctatggaaaa tgatgcaaca tacgtaccta taacaacaac tccagaaacg acagttcaaa
     1921 gtggaaaacc tacatttgat aaaccagatg ttcctttagc taaagatgcc ttttcaattt
     1981 cagatgtcta taataaggac ttcggtaatg caagtgttga tgcaaacact ggtattgtta
     2041 cattcactcc agctaaaggt gtaggagaat cggagccgat tactggaaca attcctatta
     2101 aaattgttta ccaagatggt tctgtaggca cgaccgattt agcagtaact gtaagtaaag
     2161 atatttatga aaatccagga gaaaacattc ctgcaggcta ccacaaagta accttcaccg
     2221 c
//
LOCUS       DACEPW010000287         2215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.294, whole genome shotgun sequence.
ACCESSION   DACEPW010000287 DACEPW010000000
VERSION     DACEPW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.294"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..454
                     /locus_tag="IW819_002719"
     CDS             <1..454
                     /locus_tag="IW819_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1245797.1"
                     /translation="PQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFT
                     VKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQD
                     KNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            588..1931
                     /locus_tag="IW819_002720"
     CDS             588..1931
                     /locus_tag="IW819_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1245798.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2043..>2215
                     /locus_tag="IW819_002721"
     CDS             2043..>2215
                     /locus_tag="IW819_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1245799.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT          870 a          283 c          392 g          670 t
ORIGIN      
        1 acctcaagtt gatcctatta cagagggtga ccaaattgtt actggtaaaa ctgaaccaaa
       61 tgcagaggtg acagttacat taccagacgg aagtcaacac catgggacag ctgataaaaa
      121 tggtaatttt acagttaaag ttcctaaatt agaggcaggg acaaaagtga tagtaactgc
      181 aactgatgaa tctggtaata ctagtgaacc tactaatgta gttgtttctt ccaacaaaaa
      241 agacagtgga aaaaacggta gtaaaggtag taagactgac aatcaaggta gcaacagcaa
      301 tcaagacaaa aatagaggca aaagccagtc ctcaaaagtt tttcctaaga cgggagagag
      361 tgacagtaat atctttacta ttagcggtgg tttaatactg ttaggaacgt taggattatt
      421 agggtataaa aatcgaaaaa aagagaatga atagaaaatc aatttcttaa gtcttctctg
      481 agaagaggaa gaatcttttt cttcctcttt ttgctttttt atgatgggaa ataagtgtta
      541 gttaaagatg aatatacttt ttatagaaag gaaaagaatg taattaagtg gaaaaaatag
      601 taattattgg ttcaaaccat tcagggctag ccgcagctcg atttctaaac ttaagcgatt
      661 caaattatga agttattcta atcgataaaa aatcagaaat tagttacttg gaatgtggga
      721 catcgttatt tataaaagga gaagtaagta atttagaaga tcttttttat caaaatagaa
      781 tgacactaaa ttctgagata aaaaagttat ttctagagac agaagttgac aaaattgata
      841 ttcaaaataa aaagattagt tgtgtaaaaa aagatggtac aagattctct caagaatatg
      901 acaaagttat attagctaca ggttcaaagc aaattatgct agatataccg gggaataatt
      961 tagctaatat tttttcgata aagaattttc caaatgcaaa agagattttt aaagctttgg
     1021 atgatagtac tattaaaaaa gtaggaatta ttggagcagg atatattgga gttgaaattg
     1081 ctgaagccat aagaaaaaga gggaaagaag tatatctttt cgatgttgca gatagagtgt
     1141 tgtcgactta ttacgaccgt agtttttcag ataaagttga agaaatacta tcaaaaaatg
     1201 gaatacatct ttgtctatca gaaagtccta aaaaatattt aggaagaaaa aagattgaaa
     1261 aaatagtcac tacaagtggt acccaatact ctatggatat ggtcgtacaa gctataggat
     1321 ttctatctaa tagtccaatt ggggaaaagg aactcttgag agatgttagt ggagcatatc
     1381 taactaatga ataccagcaa acaaatataa aagatatata tgctattggg gattgtgcta
     1441 caactttttt tacgtccatt aatagaagta gtgttgattt ttctatttct aatgctctaa
     1501 gaacagctta tattgcaact caacatatta acaatcaaga ttttacacct tctggttccc
     1561 aatttacaaa tggcttttct atatttaatt acaattttta cgcagcaggt ttgaatttaa
     1621 aaacagcacg attattaagt gagtctatag attatataga aatagaagat ttcattagac
     1681 ctgctttttt gagaaaaaat agtcgtgtaa aattaagaat tagctttgaa aaaaatacaa
     1741 agagaatagt aggtgtacaa ctttgtagtc aagaagattt atctggacta ttatcaatgt
     1801 tttctcttgc tatagaagaa caaattacat tagacaaatt gaaaatgtta gattatttat
     1861 ttcatcctag ttttagccaa ccctataatt ttattttaaa ggcagtaaca ggattaaaca
     1921 ttgaaacata atcgttacta aatgaaatca agaaatatac tgtattactc aattcattga
     1981 aaatatttat cataattttt aatagaaaac actattaaag agaaaaaaga gaggtaaatc
     2041 aaatgaaaaa atctttaaaa cttgcatcag cagttatatt aatgtcccaa ctccttatag
     2101 caacaccttt ccatgtgttg gcaagcgaaa attcaacata caaaattgca aaagaaagaa
     2161 ccgagacgac tgaaatgagt aattccgatg taacagcaga gagtagtgaa ctttt
//
LOCUS       DACEPW010000288         2194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.295, whole genome shotgun sequence.
ACCESSION   DACEPW010000288 DACEPW010000000
VERSION     DACEPW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.295"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            199..1623
                     /locus_tag="IW819_002722"
     CDS             199..1623
                     /locus_tag="IW819_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1245800.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPKKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVTQTKTKTEQAVLTD
                     GFLRFSMHYGFETQVCNPRSGNEKGSMENKVGYVRYNFFSSTPIMKDFASFNEALAVE
                     LSHDRERIHYEKHVIINDLWNTEKTDLLALPDTDYPVFKEIEVKVNKYNEIKLDNERI
                     HVPRVRNYSIIYGLLRFDSYQLISTDGEIISEGLRPYMMKKRAIDWQAILPDWRRKSR
                     AMHYSRYWKYLPERIKLFLSHPDWKEQEKRLNQISGLLVTHDMLGIDTHFYDMFSAST
                     TTDAYDIDWHQYDAFLSLTGEVID"
     gene            1623..>2194
                     /locus_tag="IW819_002723"
     CDS             1623..>2194
                     /locus_tag="IW819_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1245801.1"
                     /translation="MDQAKSLETACKLLRLSHVLPTLVRKDLDYSFETKEDWLMYLLE
                     KELACREELKTARLLKQAKFPNKRTLEDYEWHDHICLPNATTKDELLSLSFIHQKENA
                     VFVGTPGTGKTHLACGLGQLAAKSGIDTRFWRVSDLVNELEHHWKNNTLEVFKHRFDK
                     VKLIILDEMGYVPFTKEGAELLFQLISDWY"
BASE COUNT          700 a          420 c          478 g          596 t
ORIGIN      
        1 gagtcatcta tttcgtttat tcactttttc aagtggacat tcattaagct gtttatggca
       61 gaataaaagt acatacaagt gcaacgaaaa actacacaac tccgcaaatc gattaagtta
      121 agataaaaag ccattgcggg caacctgaaa ttcctctaaa gttatagtct cgacactgta
      181 actggaggta agaaaggtat gctcacaatg accgatatta atactatcag aaatttacga
      241 aataatcatg ataaatccat taatcatatt gcaaaggagc tagatgtaaa ttggcgcacg
      301 gctaaaaaat atgcagatga tgatttgctg ccgcaaccta aagcacgcaa aaagactggt
      361 atgatgtata cagaaaagtg gggaggcatc gtttccctat ggctttcaga agatgctaag
      421 ctgccaaaga aaaagcgtag gactgctgaa gcaatgacta ctgctttaaa agccgatggc
      481 tttccaggat cctaccgaac agtctgtgta ttcattcaag attggaaagc gacacactac
      541 ttggaaacac tggaagacca aggctttgag cgtcttgaac acccgccagc agaagcgcaa
      601 cttgattttg gtacgatgga agtttgtcac gatggagcgt ttaaagatgt gaaggtatta
      661 gtgatgactt tcccttttag taatgcagga ttcgccgttg tactccccgc cgagaatcaa
      721 gaatgtttgt tggaaggcat gaaagaactc ttcagccaat caggtggcgt tcctcggaaa
      781 atacggattg ataatatgtc aacggccgtt actcagacaa aaacaaagac tgaacaagcg
      841 gtgctgactg acggtttcct ccgattttct atgcactatg gttttgagac acaggtgtgc
      901 aatccacgta gcggaaacga gaaaggtagt atggaaaaca aagtcggcta tgtacggtac
      961 aacttctttt catctacacc gattatgaag gatttcgcca gtttcaacga ggcattagcg
     1021 gtggagctgt cacacgatcg agagcgaatc cattacgaaa aacatgtcat tatcaatgat
     1081 ctatggaaca cagaaaaaac agatttgcta gctttgccgg acaccgatta cccagtattt
     1141 aaagaaatcg aagtcaaagt caataagtac aacgaaatta aattagataa cgaacggatc
     1201 cacgttccac gtgtacgcaa ttattcaatt atttatgggc tgcttcgttt tgattcctat
     1261 cagctaatca gcactgatgg tgagattatt tcagaaggac ttcgtcctta tatgatgaaa
     1321 aaacgagcaa tcgattggca agcaattctt cctgattggc gcagaaaatc gcgtgccatg
     1381 cattactctc gctattggaa gtatttgccc gaacgaatca agttatttct cagtcatcca
     1441 gattggaaag aacaggagaa gcgtttaaat caaatctcag gtttattggt gacacatgat
     1501 atgttaggga ttgatacaca tttttacgac atgtttagtg cctcgactac taccgatgcg
     1561 tatgatattg attggcacca atacgatgct ttcttgtctc taacagggga ggtcattgac
     1621 tagtggatca agcaaaaagt ttagaaactg cctgtaagtt gctgagactt tcccatgttc
     1681 ttcccacttt ggttcgcaaa gacttagatt acagctttga aaccaaagaa gattggttga
     1741 tgtatcttct ggaaaaggag ttggcttgcc gtgaagaatt gaaaactgcc cgactcctaa
     1801 aacaagcaaa gttcccaaat aaacgtacct tagaggatta cgaatggcat gatcatatat
     1861 gtttgccaaa tgcgacgact aaagatgaat tattgagtct atcgtttatt catcagaaag
     1921 aaaatgctgt atttgtgggt acacccggta ccggtaaaac acatctagct tgtggattgg
     1981 gtcagctggc agccaaatca ggtatcgata cacgcttttg gcgggtatca gatttagtca
     2041 acgaattgga acaccattgg aaaaacaata ccttagaggt cttcaagcat cgcttcgata
     2101 aagtaaaatt aatcattctt gatgagatgg gttatgtgcc tttcactaaa gaaggcgcgg
     2161 aactgctttt ccaattgatc agcgattggt atga
//
LOCUS       DACEPW010000289         2189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.296, whole genome shotgun sequence.
ACCESSION   DACEPW010000289 DACEPW010000000
VERSION     DACEPW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.296"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(325..528)
                     /locus_tag="IW819_002724"
     CDS             complement(325..528)
                     /locus_tag="IW819_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245802.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="IW819_002725"
     CDS             complement(987..1952)
                     /locus_tag="IW819_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1245803.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DACEPW010000290         2176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.297, whole genome shotgun sequence.
ACCESSION   DACEPW010000290 DACEPW010000000
VERSION     DACEPW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.297"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            174..1742
                     /gene="liaX"
                     /locus_tag="IW819_002726"
     CDS             174..1742
                     /gene="liaX"
                     /locus_tag="IW819_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1245804.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            1921..>2176
                     /locus_tag="IW819_002727"
     CDS             1921..>2176
                     /locus_tag="IW819_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1245805.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTK"
BASE COUNT          826 a          345 c          459 g          546 t
ORIGIN      
        1 ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag aaagaacaac
       61 tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa tcagtgcata
      121 atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt tccatgaaag
      181 aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa gaaggcttag
      241 atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa gaagcagaca
      301 aggtaactgc tagtcataaa gaaaaagata aggcaagtca attgatcgat aaacttgaaa
      361 acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga caagatcaag
      421 aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact tcggcgcgtc
      481 tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag aaacaggaag
      541 cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa gaattagcac
      601 agcgtcaggc gttagaggca gaaatcaaag agttagaagc tagtggcgat acactgttag
      661 aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa tggtctgaga
      721 aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat caagcaacag
      781 atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta ggcaaattct
      841 tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa tggaaagatg
      901 tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt tactatgaag
      961 ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg ttaaaaacgt
     1021 gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa atgggtgctg
     1081 aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat catatctcct
     1141 tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct aaacgagtat
     1201 acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca ttggaagcta
     1261 aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta actgcaacga
     1321 tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac atcttagatt
     1381 ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa aatctttctg
     1441 tgtctttagt aaatggtgat gtacgtctta cgattaaaga agataatttg aaaaaagtag
     1501 aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc ggtttggaag
     1561 gacatgctaa aacaagtctt ggaagtatca atagccgtct gtccaattac gaagtagttc
     1621 gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca gatgatgaga
     1681 tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat acagataaat
     1741 aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt gtaaaatagc
     1801 ttcccctatt ttacaaaatt gtgccttatt gccgataggc tatctcttga acaccgatta
     1861 agaagaaggt tgtcacaacg attttcttca tttaaaatag tagaacagag gtgagacatg
     1921 atgaaaaaaa agttgacaaa atccaataaa aatgttgttt tgacaggtac tttagcaggg
     1981 attggggaat atatcgggat tgatcccact gttatccgtg tgctttatgt attcctaagc
     2041 ttagttgctt ttggaagtcc aatcatttta tacattttat tagctttgat tatcccatct
     2101 gcgcgttctg cacgaacaaa ctatggacat aacaatgcat attatgaaaa aaacaattat
     2161 cgtgaacaaa caaaaa
//
LOCUS       DACEPW010000291         2156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.298, whole genome shotgun sequence.
ACCESSION   DACEPW010000291 DACEPW010000000
VERSION     DACEPW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.298"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            236..1876
                     /locus_tag="IW819_002728"
     CDS             236..1876
                     /locus_tag="IW819_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1245806.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
BASE COUNT          719 a          403 c          418 g          616 t
ORIGIN      
        1 tctatttatc tatctaacat atcctttatt cttctacttt tctttcttta gcatataaga
       61 gatacgcata gtaatcaagc aaggattgtt gatataaaaa gaacaaatac cgctattttg
      121 atttcttttg actctattac tcatataaat aaaatttatt atacctgctc tttttgacta
      181 tatcctcatc tgactcctat acttcgtttg tattaaaact taggaggaat cattcatgct
      241 tacagggcta aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga
      301 agaacgggca aaatacggaa tcactggtat gcttccaagt actgtccaaa cattggaaca
      361 acaatctgtc caagcttacg gtcaatactt aagcaaacag accgatttgg aaaaacgtat
      421 cttcttaatg aatctattca atacgaaccg gacattgttt tataaattga tgggcgaaca
      481 tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata
      541 caacgaaatt ttcttgaaac cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa
      601 tatcaaagca agtttgaaaa acgcagcaga tggacgcgac attcgtttga tcgttgtgac
      661 agatgcagaa ggaatcttag gtatgggtga ctggggtgtg aacggtgttg acatcgctat
      721 cggtaaattg atggtctaca ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt
      781 atcaatcgac gctggtacta ataacgaaga attattgaat aatccattat acttagggaa
      841 tcgtcatgct cgtgtagaag gcgaaactta ctacgaattc attgatcaat tcgttcaatc
      901 agctactgaa ttattcccag aattgttatt acattgggaa gattttggac gaggaaatgc
      961 tgcaacgatc ttagaaaaat acgaagataa aatcactact ttcaatgatg atatccaagg
     1021 cacaggaatc gttgtactag ctggtgtatt aggtggattg aatatatcag gtgagcaatt
     1081 gaaagatcaa acgatcttga catttggtgc tggtactgct ggtgtaggta tcgcaaacat
     1141 ccttttagat gaaatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta
     1201 tcaagtagat aaacagggtt tattgtttga agatacaaaa ggattgactc ctggtcaaat
     1261 cccattcgct cgtaaacgct cagaattcac taatgctgat gaattgacaa acttagaagc
     1321 agttgtaaaa gcaatccatc caacgatcat gatcggtact tctacccaac ctggagcatt
     1381 cactgaagaa atcgtgaaag aaatggcggc tcatacccca cgtccaatca tcatgccttt
     1441 gtctaatcca acaaaattgg cagaagcaaa agcaaaagat ttgattgaat ggacggacgg
     1501 aaaagcttta gttggaacag gtatccctgc tgctgatgtg gaatataacg gtgtaactta
     1561 ccaaatcggt caagcaaata atgcgttgat gtacccaggt ttagggcttg gtttgattgc
     1621 ttcaacagct acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg
     1681 aatcgttgat gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga
     1741 attctctcaa acgattgctg aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg
     1801 tgaagaaatc acggatatca aagaagctgt tgaatctgct aaatgggttc ctgaatataa
     1861 atctttagaa gattaagatc gttatagtaa aagaacatac agaaactttg tatgacgtct
     1921 aaaaagaaaa aagcgactgc ggcaaaacag cagccagcca actactgctc ttttgccgct
     1981 tctctttggc ttttttcata cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa
     2041 aacaacaacc gcaactgcag ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat
     2101 taccggcgta agtttgcccc tttatttgat catggtcctt gtcctgatca ttgcaa
//
LOCUS       DACEPW010000292         2151 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.299, whole genome shotgun sequence.
ACCESSION   DACEPW010000292 DACEPW010000000
VERSION     DACEPW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.299"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            153..1142
                     /locus_tag="IW819_002729"
     CDS             153..1142
                     /locus_tag="IW819_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ1245807.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            1431..2129
                     /locus_tag="IW819_002730"
     CDS             1431..2129
                     /locus_tag="IW819_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ1245808.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT          767 a          341 c          446 g          597 t
ORIGIN      
        1 aatcaatttc tgatctatca acgagtaaaa aacaaatggc aaaaaaatat acgaaaaaat
       61 cattttgatt aactcctttt ctttaaaatg tagtaaacta aaaatagaaa aaaagaaagc
      121 gttttaaaaa gatttgatga ggaggagaaa aattgtatat tgcagatagt gcacgttatg
      181 aaaaaatgga atatcgtaaa tccggtcatt caggtttgaa attaccgatt ttatctctcg
      241 gattatggca aaattttggt gattatgatc ctatccataa tcaaagagag attttaagag
      301 gcgcattcga catgggaatt acccatttcg accttgcgaa taattatgga ggccctgctg
      361 gagctgcaga gaaaaacttt gggcgtattt ttagagaaga ttttcaagcc taccgtgatg
      421 aactgattat ttctagcaaa gcaggttatc atatgtggga agggccatat ggggaatggg
      481 gctctcgtaa atccatcatt tcaagttgtg accaaagtct ccaaagaatg gggctagatt
      541 atgttgacat cttctatcat catcgcccag accctgatac acctttggaa gaaacagcag
      601 aagctttgat gcaacttgta agacaaggaa aagcattata tattggtata tccaactata
      661 atggagaaga tacaaaaaaa atgactgaaa ttttaaaaag aaaggaagca ccgttcatta
      721 ttcatcagat gcgctacaat atgttttctc gtgcactact agaagacgat ctatctccag
      781 tattagaaga agaagggcta ggggcaatca cgttcagtcc gcttgctcaa gggttgctga
      841 caaatcgtta tctacatggt attcctgaag attctagagc acatcgtaag gaaataccat
      901 tcctttcaga agaacaagtt ggatcaactc tggaaaaaat caaagcactg caaacaatcg
      961 ccgtatcacg aggacaatca ttagcgcaaa tggcccttgc atggaatttg cgacaaaaat
     1021 ctgttacaag tgtactggtc ggagcaagtc ggttaagtca gttacaagaa agtgttcgaa
     1081 tgatggataa tttagatttc tcaccagaag aagaattgag aatcgaccaa attctagaat
     1141 agaaacgatg taatcaaact ttttatccat atattaaaaa aaacaaaaga tgacatcaag
     1201 aatcgaatga agatcgtctg ttattgaaca tcatattttt tgtcgttcaa aataaaaatg
     1261 atcgaaggct aggagtgaat tttatcctag tcttttttat ggtaaatatc ttcttcgttt
     1321 gtatgtctat gaacggatta atcaattttg tatacaaaaa atcatcagat gattcaaacg
     1381 tgttataata attacgaaaa aatacatcag atgaaaagga ggtatgtggt ttggaagaaa
     1441 ataaaaccct agtagaagta actactgaca agatccttag atatttaaag gaaatgcagt
     1501 tgcaagtagg agataaactg ccgaatgaat atgaattgtc cgattattta gaggtggggc
     1561 gaagcacggt gcgagaagct gtgcgggctc tagcttccag gaatatcctt gaagttcggc
     1621 aaggagcggg gacatttgtc agtgataagc gagggattgc caatgaccca ctcggttttt
     1681 ctttgattga agatcaggat cggatgatac gagacttatt tgaactgaga tatttattgg
     1741 agcctaagat ggcaggtttg gcagcggaat atgcaacgaa gaatcaaatc gtccaaatga
     1801 aaaaattaac gcaaaagatt gaaacttctt ttcgagaagg cacacaagaa catgttcaat
     1861 tagacatcga ttttcatacg atgattgcag aagctagcgg aaacgtagcc ttgctgcata
     1921 ttatcccaat cattaatgaa tcaattgctt tgtttaataa aaattatgat atccagcatc
     1981 tgaaaatcga gacgatcgat attcatacag agatcgttca ggccatcgaa aggcggaatg
     2041 cacttgctgc gtttgatgca atgactgtcc atatggcaat aaatcggaga gaattacaga
     2101 aaataatagg agaaaaagaa actaattgac agttcatctg atgtatttta t
//
LOCUS       DACEPW010000293         2135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.300, whole genome shotgun sequence.
ACCESSION   DACEPW010000293 DACEPW010000000
VERSION     DACEPW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.300"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..468
                     /locus_tag="IW819_002731"
     CDS             <1..468
                     /locus_tag="IW819_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245809.1"
                     /translation="PVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLR
                     KNETEYQEVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPR
                     ECWRLQGFPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            493..747
                     /locus_tag="IW819_002732"
     CDS             493..747
                     /locus_tag="IW819_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245810.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            1003..>2135
                     /locus_tag="IW819_002733"
     CDS             1003..>2135
                     /locus_tag="IW819_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1245811.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDR"
BASE COUNT          673 a          379 c          520 g          563 t
ORIGIN      
        1 ccggtagatg atggaatttc tgtagcgggt atgttactgg gaaattttga acaaggcaat
       61 cgggtttatg agataactgg aacagcgcct actttgtcaa cgaaacaagg cggaacgaag
      121 atcatgcttc ggaaaaacga aacagaatat caagaggtga agccagggaa cagtgtgaac
      181 ctggcttttc ctaattccac aagtcgtcga ggccgtctgg gaaaacaaag tgtgcatacc
      241 ttattaacag gagaccaaca agcagtcgtc acggatcagt atcaaattcg aaagttaaca
      301 cctagggagt gctggcggct tcagggcttt ccggactggg cttttgatcg agcttctcaa
      361 gtcaattcag atagtcagtt gtataagcag gctggaaatt ctgtaacagt tccggtcatt
      421 tttgatattg ctagaagatt aaaggaggta aaaacagatg atttatgatg agttgattgg
      481 ggaatctact gggtgattgg caagattcag tcagatccgg aattggaaga agaactgtac
      541 cggctcaatt ttgatattcg caaaaacggg gtgaaagtcc ctgatgatcc gtatgtaatt
      601 gatgaggaaa cggatgctcg aatggagctg aatcaagtca tcagtgaatt ggaacgaatt
      661 gcggactttg ctaaagaacc tgatatccgc caatacttgt ttgagataaa agcagagttg
      721 gaaattgagg gtattaccgc ggagtaacgg ccaaataaac cactgatagg cactgaccgc
      781 gggggatttg cgaagcagaa atcctccgtg gtgctgtgcc tgtttatgga cggcgttagc
      841 cggccatgat gtctcacccc ccgtatctaa caggggggta caaaaacaca aaaaataagc
      901 aagcaacgat aaggaatggc tgtcctatca aggataataa gcaatttgtg tagatgttaa
      961 ctgatttgaa aaagttaaca ccttaaattt ggaggtgaga acttggcaca aaggaattta
     1021 gattatcggc tattaaaaga ccggcgtaat gaatatggcg tttcacaaaa taaattagct
     1081 acgacttgtg gacttagtcg cccgtactta aatcagatag aaaatggtgg cgtaaccgca
     1141 tcgacaaaaa ccatgaggaa aatttttgac caactggaaa gtttcaatcc cgagttacct
     1201 ttaacgttac tgtttgacta tgtaaggatt cgctttccca caacggatgc gaggaaaatc
     1261 attcaagaga ttctccattt gaaatttgat tatatgctcc acgaagatta cgccttttac
     1321 tcctatcaag aacaatatgt tatgggagat attgtggtga tgttgtcaca tgaagaagat
     1381 aaaggcgtcc ttttagaatt aaagggtcgt ggttgtcggc agtttgaaac ttttttactc
     1441 gcccaaaagc gtagctggta tgactttttc gaagattgtc taaaagccgg tggcgtgatg
     1501 aaacgcttag acctggcaat caatgatcga gttggcctgt tagatattcc tgatttaacg
     1561 aaaaaatgtc agaaggaaga atgtatctcc ttgtttcgta ccttcaaaag ctatcgttcc
     1621 ggggaactgt tgaaacctga tgataaagat ggtatgggca atactttgta tattggcagt
     1681 cttaagagtg aggtctattt ctgtttgtac gaaaaagact atgagcaata tatcaagtta
     1741 gggattcctt tagaccagac cgaaacgaaa aatcgttttg aaatccgact gaaaaatgat
     1801 cgagcttatc atgcaattca agatttgtta aaaggtcgca gtattgaaag taccacattt
     1861 tccattatta accggtactt gcgatttgcg gataaggtgg aaggcaaaag gcgaactaac
     1921 tggcccttga atgaacaatg ggggcgcttc attggacgga atcagaagga aattcaatta
     1981 acttctgaac cgaagcctta taccattgaa cgaacgttga actggcttgg acgacaagtc
     2041 gcccctacgt ggaagatggc caaggagttg gatcggttga atcagacaac gtacattcag
     2101 gatatggtaa ataatactcg actatcagac cggca
//
LOCUS       DACEPW010000294         2120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.301, whole genome shotgun sequence.
ACCESSION   DACEPW010000294 DACEPW010000000
VERSION     DACEPW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.301"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            888..1361
                     /locus_tag="IW819_002734"
     CDS             888..1361
                     /locus_tag="IW819_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1245812.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1370..1597
                     /locus_tag="IW819_002735"
     CDS             1370..1597
                     /locus_tag="IW819_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1245813.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          684 a          338 c          315 g          783 t
ORIGIN      
        1 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
       61 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      121 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      181 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      241 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      301 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      361 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      421 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      481 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      541 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      601 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      661 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      721 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      781 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat
      841 aattattctg ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa
      901 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
      961 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
     1021 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
     1081 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
     1141 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
     1201 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
     1261 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
     1321 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
     1381 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
     1441 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
     1501 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
     1561 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
     1621 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
     1681 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
     1741 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
     1801 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
     1861 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1921 gtgcttaaaa cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta
     1981 agctcccata tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta
     2041 ctatgtagtt ttgataaaat gatttgaata acagtgatgg acaattcttt tttattttta
     2101 acattatttt gataataatg
//
LOCUS       DACEPW010000295         2113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.302, whole genome shotgun sequence.
ACCESSION   DACEPW010000295 DACEPW010000000
VERSION     DACEPW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.302"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..237
                     /locus_tag="IW819_002736"
     CDS             <1..237
                     /locus_tag="IW819_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1245814.1"
                     /translation="PAEKIATAKLHLQRLADLKGEKVACREFRTHAAYYLKGIPRAAK
                     VKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL"
     gene            348..1844
                     /gene="lysS"
                     /locus_tag="IW819_002737"
     CDS             348..1844
                     /gene="lysS"
                     /locus_tag="IW819_002737"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1245815.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
BASE COUNT          782 a          361 c          441 g          529 t
ORIGIN      
        1 ccagccgaaa aaattgctac agctaaactt catttacaac gcttagcaga tctcaaagga
       61 gaaaaagtag cttgtagaga attcagaaca catgcggctt attatttaaa agggattcct
      121 cgagcagcga aagtgaaagt agcaatcaat cagacagaaa aacagcaaga aatcaatgac
      181 ctgctggatc aatttgtaga aaaagcggaa aaagtactat tgaaacaaac gttgtgattt
      241 ctaagttttt cgtgaatttt caagccgaaa ttccaagttc ggcttgcttt ttttgtgttt
      301 cattggcatt ataggaaaga gattaacatg agaatggagg aacttttgtg acacaggaac
      361 aacaacatca agtagaagat ttaaatgatc aaatgcttgt acgtcgtcaa aaaatggaac
      421 aattgcgtga agatggaatc gatccatttg gaaaacgatt tgaacgcacg cacaactcag
      481 aagaattgca tgaactattt gatccaagaa caaaagaaga actagcagaa atgggattga
      541 ccgctagtgt agctggtcgt atgatgactc gccgaggaaa agggaaagct ggatttgcgc
      601 atctacaaga tcgtgaaggc caaattcaaa tttatgtaag aaaagaccaa gtaggtgatg
      661 aggcctatga agtattcaaa catgcagacc ttggagattt ctttggtgta actggacaaa
      721 tcatgaaaac agacacaggc gaagtttcta tcaaagcttc agaaatcacg attttgtcaa
      781 aagcacttcg tccactgcca gataaatacc atggcttaac gaatattgaa caacgctaca
      841 gacaacgcta cttggattta atcagtaatc gcgaaagctt tgaccgattt atgaaacgaa
      901 gccaaattat cagcgaaatc cgtcgctatt tagatggcaa tggctatatt gaagtagaaa
      961 caccagtatt gcacaatgag gcaggtggag ctgccgcacg cccatttatc acgcatcata
     1021 atgcactgga cattgattta tatttgcgta ttgcgctaga acttcattta aaacgactaa
     1081 ttgttggcgg aatggaaaaa gtttatgaaa tcggccgcgt tttccgtaat gaaggaattg
     1141 atactacaca taatccagaa tttacgatgt tagaagcata cacagcatat actgatttca
     1201 atgatgtaat gaatctgaca gaaggtatta tccgtaacgc agctgaaaaa gtactaggaa
     1261 cagcaaaaat cacatatgat ggacaagctg tcgacttaga aagtgacttt aaacgcatcc
     1321 atatggtaga tgcaatcaaa gaacaaactg gtgtagactt ctggcaagaa atgacattgg
     1381 aagaagcatt agctttagcc gaaaaacaca atgtagaagt aaccgaagct atgggtgtcg
     1441 gacatgttat caatgaattc tttgaaacat ttgttgaaga aacattgaca caaccgacgt
     1501 ttgtatacgg acatccagtc gaagtttcgc ctttagcgaa gaaaaatcca gaagatcctc
     1561 gattcacaga tcgattcgaa ctattcatag tggggcgcga atttgctaat gcctttacag
     1621 aactgaacga tccaattgat caaagagaaa gattcgaagc gcaagaaaaa gaacgtgaac
     1681 taggaaatga cgaagctcac ggtgtagacg aagacttctt ggaagcctta gaatacggta
     1741 tgccgccaac tggtggtcta ggcatcggga tcgacagatt agtaatgttg ctaactgatg
     1801 cacaatcaat tcgggatgtt ttgctattcc caacaatgag ataatcgaat aaaagctgtc
     1861 taaggaattc cttaggcagc ttttgctgtg tagaaaggaa taaataataa cgaaagttca
     1921 aaagctaatg ccgactattc tagacattaa agaagacgaa aacgaacgat aaagaaagaa
     1981 aagttatgaa taatcgttta ttgaaaaaaa cttgaaaaaa tacaaaaaat tcgttgacca
     2041 agctaaattt agttggtata ttattacttg tccaaaagac agaagaaaat atttattcaa
     2101 aaacataaca aaa
//
LOCUS       DACEPW010000296         2112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.303, whole genome shotgun sequence.
ACCESSION   DACEPW010000296 DACEPW010000000
VERSION     DACEPW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.303"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..1705)
                     /gene="treC"
                     /locus_tag="IW819_002738"
     CDS             complement(77..1705)
                     /gene="treC"
                     /locus_tag="IW819_002738"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1245816.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
BASE COUNT          580 a          424 c          364 g          744 t
ORIGIN      
        1 aagattattt taaataaaac acaaaacgtc gatcttacga gaatagctcg taggaacgac
       61 gttttgtgtt tttgttttaa tttttcagca aagcaattgc ctggtatggt tttagctcca
      121 gactttgatg aagaacagta tcggaataat tatcgataag aatattggct tctgtgaatc
      181 cttctgggat ggcgatcctt gtatctttat catagaaatt tgttaataca agcagctgct
      241 gtccttcaaa ttggcgaaga aagccataaa cttccggatg atttgcttca taagcatgat
      301 aatctccttg tgcaacgatt ggatatgttt ttctcagccg aatcaatttc ttgtaatagg
      361 acaaaataga atcttcagaa gatagttctt tttctacatt aatctcttct agcttgcctc
      421 caacttttaa ccaaggtttc cctgtagtaa atcctgcttg ttctgtgtct gaccattgca
      481 tgggtgttcg agagttatcg cgagattttg ctttgatccg acgaaacgct tctttttctg
      541 tgaatccttg ttcaaggagt aacttataag cattcaaact ttctacgtca atatagtcgg
      601 aaatcgatga atagtctggg tcgatcatgc cgatttcttc tcccatataa atatagggcg
      661 tcccacggtt caaatggatc atggttgcta gcatctctgc tccttgtttt cgatactgat
      721 cgatagctac aaatcgattg agcgcacgtg gctgatcatg attattaaaa aatagagcat
      781 tccagccgtt accctcactc atcttttctc cccatgtatg aaatagtcgc tttaattctg
      841 caaaatcaaa tggaggagta gaccatttat tcccattttc ataatcgact ttcaaatgat
      901 ggaaattaaa gaccattgat aattcttctc tactaggttc tgtatacaag atacaatttt
      961 cgatcgtcgt agagctcatt tcaccgactg tgatgatagt ggaatccgtc ccaaacgttg
     1021 cttggttcaa ttcttttaga tacgtatggg tgattggttt gtccgtatat tcaggtttgc
     1081 catcattttc tttattattt ttcaattgtt cgtctttacc gataacattg ataacatcga
     1141 aacggaatcc ttttacccct ttttccatcc aaaaacgaac aactttgaat agttcctctc
     1201 ttactgcttg gttccgccag ttcaagtcag cttgtgtccg atcatataag tgcaaataat
     1261 atttccccgt atctccaaat ggcgaccaag cacttccacc aaattttgat acccaatccg
     1321 ttggctcttc tcttaaaatg aagaagtcct gataatgctg gtcccctgct agtgcttttt
     1381 tgaaccattc atgttctgtc gaaacgtgat tcagcaccat atcgagcatg atttctatcc
     1441 ggtatttctt tgcttcggct attagctgtt cgaattcctc catcgtacca aagataggat
     1501 cgatggctgt gtaatcagag atatcataac cgttgtcttt ttgagggctt ggataaatag
     1561 ggtttagcca aatcatgtcg atgcctagat cattcagata cgggagtttt tctttgattc
     1621 cttttaaatc tccaattcca tcaccattac tgtctttgta agatttggga tagatttggt
     1681 aaatgacttt gtctttaaat gtcatggata tttacctcca ttcaactgat ttattaccta
     1741 ccaaaactta tatatacaag ttatcattta ttattataac tgaaccgaaa gcgtttgtaa
     1801 aatttatctt tatacattct aagaggtctg ttgcccaata aaaaacaaga gatcagacat
     1861 gtatttgtct atcctcttgt ttaaaatagc aaaatattct tgtttcttct ataaatggtg
     1921 ctcaaaagct tttatctacg gcaatcagtc ttctcaaatg taaaacatga ggagctaatt
     1981 ttatttgagt attctttttg ctcgatgcag gacagctttt tcacaacctc tgattcacgg
     2041 tgttcaatta gcagctgacc ctcgttatta gctcaaaacg ctgatttcac aacctcttcc
     2101 ttaagcggtg tt
//
LOCUS       DACEPW010000297         2110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.304, whole genome shotgun sequence.
ACCESSION   DACEPW010000297 DACEPW010000000
VERSION     DACEPW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.304"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..1036)
                     /locus_tag="IW819_002739"
     CDS             complement(137..1036)
                     /locus_tag="IW819_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ1245817.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1046..1402)
                     /locus_tag="IW819_002740"
     CDS             complement(1046..1402)
                     /locus_tag="IW819_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1245818.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1757..2053
                     /locus_tag="IW819_002741"
     CDS             1757..2053
                     /locus_tag="IW819_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1245819.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          660 a          407 c          359 g          684 t
ORIGIN      
        1 aaatcatccg atcaaataaa cattcgctga ttattttccg caaaaaaagc agcatatcca
       61 cgataaatgg atctactgct cttacttatc cttatgtttt acaaggtatt ttcttttaca
      121 cattactaat gctgtctcaa tacggaacga ttgtcatcgc ttgttcaaat tcctcttgca
      181 aatgttgtgt ttgataggcc tgaaaagctg ggtgatgggc acgcaagagg acacgtaatt
      241 tcataggatc atagctgtct agcaactgtt ctttcaagac aaaatctcgg gtcagctgcc
      301 attcataata ataaccaaaa ggtctgcagc tgtaaatcgt gtgaccgatg gatttatctt
      361 caaagcgacg atgcgtatgg ccaaaaacta cttgtttgat atttgaataa cggtcaagta
      421 attctccgaa tgattctgat cctaaaaatg catttaattt gttccaacgc tcataaggag
      481 cagattgata catgataaat gcccgtttag gaacgaaatg agtggataaa atcacctcaa
      541 gtttttttgc ttccagctgg tctagcaaat tttttgcagc tgcaagcatt tctgcattga
      601 cctcgagatc tgttttccca cgctggatga accgatcata ccaatacaaa cgcttcattc
      661 gtacgattgc ctcgtaatct ttcaattcgg aaaactgata gtcgtaccag ccattcatcc
      721 ctaaaagaac ggtctgctca tttaaagcaa tataccgttc atttaaaaaa tgtgggtctg
      781 gaaaatggtc catcatctgt tcattgggca catctgccaa ctcgtgattg ccgaaatgaa
      841 atgtcgtaga taatccttta ttgacgaaaa attcgacaac agctaggcaa cggtctactt
      901 tattagccgt atctccggct agatgaagcc gagtgatctt gtgcttttgc aaaacttgcc
      961 aaagctgcat cagctctgat tcgccgaatt tattgatgtc cacatgtagg tcgctgatga
     1021 tcgccaattt tcccatttta tccctctagt cttttcgaac aacttgcaag ttgccttttc
     1081 tcaagccgct tggtcctttt aattgtaaac tattataagt tggttctaaa gcaagtgtgg
     1141 atggatcatc catgtaaagt attgcactgt ctttgatttt gattggtcca actggtgtat
     1201 cagccactac agaataaaga cttagatctg tttgtgtatc ttttttgaca atgatcaatc
     1261 gaaatgccgt atccaattgg caactgactt ggctttctgc aaatggaccg tcaccgtttt
     1321 cgatatctaa taaaagttga tcgtctgctt ctaatttatt cttcaatact tcttgtgctt
     1381 cttttgtcac ttctaatttc atctgtatca cctcatgtca tagaataatc actttattca
     1441 tcagatgcaa ctaatttgtc tggttctgac tgttctcaat ttattggaaa gtaagtcatt
     1501 cgtttattat gatttctctt atcacaaaag agacaacttt cccttttctt cgttcagttt
     1561 cttgaacgga aaaagaccca aaaagcaacc tgttatttgt ttaaccttat tcctttctta
     1621 aataacaaaa aatactataa aaaataaaaa acctttaaca actattggaa aaagaattac
     1681 tttttgttca cggattatta acaactcttc ggtagagtaa acttatcaaa taaaagactt
     1741 ctttggagga atcactatga aggaatatat aaaaaacatc tattttatcg aagaaacaca
     1801 aaatatcgag ggttcttata ttgaagtaaa aacactcttt gtcaatgaag ataaaaccaa
     1861 agcattggat atttataaaa aattagcttc aaaaaaaacg aatagttttg gattgatatt
     1921 aagcgagtat aaaatcaaag cagaagagag ttatttctat caattattaa aacggtggag
     1981 taagcttccc gccgattttt atcgaaaaat gcagatcatc aactatcaac cgcttgctga
     2041 gacacatgcc taaatagagg ttgtgaaatc agtgctttga gctaataccg aaggtcagct
     2101 gattgaacat
//
LOCUS       DACEPW010000298         2109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.305, whole genome shotgun sequence.
ACCESSION   DACEPW010000298 DACEPW010000000
VERSION     DACEPW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.305"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..589
                     /locus_tag="IW819_002742"
     CDS             <1..589
                     /locus_tag="IW819_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAQ1245820.1"
                     /translation="LICHCSGAISSDIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLK
                     KSFITIEGDEKYAKFLCDFFKKLGNSTVIVSKENKSLYHAASVVSSNLVLGLINTSVT
                     YLIQCGFTEKMAIEALYPLIEFNIRNIKEKGIIESLTGPVERCDISTIKGHCEVLSDE
                     DRALYMLLSKNVLEIAMLKNINRDYSELEKYLGES"
     gene            601..1428
                     /gene="panB"
                     /locus_tag="IW819_002743"
     CDS             601..1428
                     /gene="panB"
                     /locus_tag="IW819_002743"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAQ1245821.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1443..>2109
                     /locus_tag="IW819_002744"
     CDS             1443..>2109
                     /locus_tag="IW819_002744"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAQ1245822.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLK"
BASE COUNT          811 a          229 c          434 g          635 t
ORIGIN      
        1 attaatctgc cattgcagtg gtgcaatatc ttcagatatc ttttcaaata tacaggacta
       61 tggtgcttat ggctattctg tccatcctat gttttcaata tcagataaat ataattcata
      121 caaaaaatta aaaaaatctt ttataacaat tgaaggcgat gaaaaatatg caaagttttt
      181 atgtgatttc ttcaaaaagt taggtaatag tactgtaatt gtatctaagg agaataaatc
      241 tctttatcat gccgcatcag tagtatctag taaccttgtg ctaggactta ttaatacaag
      301 cgttacatat ctaattcaat gtggatttac tgaaaaaatg gcaattgaag ctttatatcc
      361 attaattgaa tttaatatta gaaatataaa agaaaaagga attatagaaa gtttgacagg
      421 gccagttgaa cgttgtgata tttcaacaat taaaggtcac tgtgaagttt tatcagatga
      481 agacagagct ttatatatgc tattatcaaa aaatgtatta gagatagcta tgttaaaaaa
      541 tattaataga gattatagtg aattagaaaa gtatttagga gagagttagg agagaaatag
      601 atgaaaaata cagcagttac atttaaagaa tctaaattaa gaaatgaaaa attaacaatg
      661 cttacagctt atgactattc aacagcaaaa ataattgatg aagctgggat aaatggaata
      721 ttagtaggag attctcttgg tatggtatgt ctaggacatg aagatacact ttcagtaaca
      781 atggaagaca tgattcatca tactagagca gttacaagag gagctaagaa cactttagtt
      841 gttgcagata tgccatttat gtcttatcaa acttcagtat atgattcagt agttaatgca
      901 ggaagattaa taaaagaagg acgagctcag gttgttaaat tagaaggagg gatagaagta
      961 tgcgataaaa tagaggctat agtaaaggct tctatacctg ttatggcgca tataggttta
     1021 acacctcaat ctgttaatgc atttggagga tttaaggtac aaggaaagga caaggaagct
     1081 gctaaagaat taataagagc tgctaaagct gtagaaaaag caggagcatt cgctgtagtg
     1141 ttagaatgtg ttccaactaa gcttgcagag ttaatttcta aagagatatc aatacctaca
     1201 attggtattg gagcaggtgc agggtgtgat ggacaaattc ttgtatatca agatatgttg
     1261 gggatgtata gtgattttac ccctaaattt gtgaagaaat atgctaattt atcagaagag
     1321 atgaataaag catttacaaa atatatagag gaagtaaaag atggagtttt tccaggtcca
     1381 gaacatgggt ttaaaataag tgatgatgtt ttagaaaaat tatattaaaa gggtcaagaa
     1441 atatggttat atttaaaaca gtgaatgatg ttagaagtca agtaaaagag tggaaaaaac
     1501 aaggcttaaa ggtaggatta gttcctacta tgggatattt acatgaaggt catgaaagtt
     1561 taattagaaa agcatcaaaa gaaaatgata aagttgttgt aagtatcttt gtaaatccaa
     1621 ctcagtttgg gaaaaatgaa gatttaggta gttatccaag agacttagaa agagatatag
     1681 aagtatgcac aagaggtaga gcaacagcaa tatttaatcc tgaagttgaa gagatgtatt
     1741 gtgacaatgc aagtactttt gttaatataa ctggattaac agaaggttta tgtggagcta
     1801 gtaggccaat acattttaga ggggtttgca ctgttgtaag taaattattt aacataatac
     1861 cagcagatag agcttatttt ggagaaaagg atgcacaaca gttagctgta attaaaagaa
     1921 tggttaggga ccttaatatt gatatagacg ttgtagggtg tccaattata agggaagagg
     1981 atggactagc taaaagttct agaaacacat acttaagttt agaggaaaga tcttcagcta
     2041 ctattttaaa taaatcttta actttagcta aggaagcttt aaataatgga gagagagaca
     2101 gcttaaaaa
//
LOCUS       DACEPW010000299         2106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.306, whole genome shotgun sequence.
ACCESSION   DACEPW010000299 DACEPW010000000
VERSION     DACEPW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.306"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..302)
                     /locus_tag="IW819_002745"
     CDS             complement(<1..302)
                     /locus_tag="IW819_002745"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1245823.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKE"
     gene            771..2072
                     /locus_tag="IW819_002746"
     CDS             771..2072
                     /locus_tag="IW819_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1245824.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          666 a          426 c          374 g          640 t
ORIGIN      
        1 ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag
       61 ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct
      121 aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga
      181 aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgctc gacttgttcc
      241 agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc
      301 atctttattc ctcctaaagt gttcccttga ttgttaaaca aaaaaaatct ctagcgacgg
      361 catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta
      421 aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc
      481 aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa
      541 gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta
      601 tatcagaaaa aaaaacgaaa ggctagtcgt ttcataaaaa aaaaaggctc tataatttct
      661 aggactgata gaaaatcatt ctatcggtcc cttttgagca tgaaaaaaca aaacatacag
      721 aagctccgtt agaattgaat caccacaaca caatcaaagg agcgttctgt atgcctgatc
      781 ttcatcatat caaactacta cttggaatca aagataaaaa cattttcatc acaaatgtgg
      841 aatcgaagag cattaagaat atcaagagtc ttgttgtttc tgcgacgtta agtaaagaaa
      901 ttcgtcgctg tcctttgtgt aaacagatga atcatgaagg catgatcgtt aagaacggaa
      961 agaaaaaatc gctcattcaa cttaataaat gtgcgaacca actgacttat cttgctttag
     1021 cgaaacaacg ctaccactgt cgtggctgcc acacctattt tacggcgaat acctacattg
     1081 tcgatagaaa ctgctttatc gctaaacaag tgcgctataa aattttagaa gagctaactg
     1141 aaaaacaagc aatgacgaca attgctaagc attgcggcgt ttcttggtcg accgtttctc
     1201 ggacactcgc ttctctgatc cccatgacaa aagtaaaaag aaattggtta ccgcgttgtt
     1261 tattaatgga tgaatttcgt tcactgaaaa atcaagtcgg tccctactcc ttttcttgta
     1321 tggatggcga tactggaaaa ttactggata ttcttccttc acggaagaaa aaggctctcg
     1381 tttcttattt tatgcaattt gaacgacgcg cacgattgaa ggtaaaggtc atcgtgacgg
     1441 acatgaatgt ttcttatgcc tctctgataa aggaatgttt tccaaaggca aaactcgttg
     1501 tggatcgttt tcacattgtc aaacatctca ttcgtaaatt tgaggatatt cgcgtacgaa
     1561 tcatgaaaag ctttgatcga aacgatccaa cacaagccaa atattatcga caattaaagg
     1621 cgttgtctcg gcttctgatc aaacgacagg acaagcttgt ttatgacaaa tggaccaaat
     1681 ggcgcaattt tggttgggcg tatctcaccg aaagtgaagt ggtggatcgt ctgctttcga
     1741 tctctgacga attacgaatc gcctattttt actatcaaga aattcttcaa gcgtttcacg
     1801 acaaagaggc ggacactttc tttaaacttg taagaacgat gcccaattcc gttccaaaag
     1861 aactgcattc tataaaaaaa gcttttataa actatgaatc cggcattcgt ttagccctcg
     1921 aattgcctta ttcaaatgcc aaaatagaaa atctgcatac ccacatcaaa gcattgaaac
     1981 gagtcgctta tggctttcga agctttcgga aaatgaagac cagaattttc ttactaaata
     2041 atctgattac ctatgaatca aaaaacatct aaagtagaaa aggaattcta ccttagatgt
     2101 tcttat
//
LOCUS       DACEPW010000300         2102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.307, whole genome shotgun sequence.
ACCESSION   DACEPW010000300 DACEPW010000000
VERSION     DACEPW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.307"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(304..579)
                     /locus_tag="IW819_002747"
     CDS             complement(304..579)
                     /locus_tag="IW819_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1245825.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            686..1450
                     /locus_tag="IW819_002748"
     CDS             686..1450
                     /locus_tag="IW819_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1245826.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1573..2076
                     /locus_tag="IW819_002749"
     CDS             1573..2076
                     /locus_tag="IW819_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1245827.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
BASE COUNT          665 a          392 c          453 g          592 t
ORIGIN      
        1 aaaactaagc gactttttca caactagtcc ttaatgcttg atgcagatca gctttttcgc
       61 aaccttatcg acggtgttca aaagcttgca tctacggcat taagtccagc ctgttgaaaa
      121 atatggagag ctatttttca caaccagtcc ttaatgcttg atgcagacca gctttttcac
      181 aaccttattg acggtgtttt atcagctgcc tctcggtatt aagtcattac atttcaaaat
      241 atgagaagct atttggaaaa tgtttctcct taatactcga ggcaaagcgc ttcaaaaaca
      301 accttatccc accacatcaa atttctttcg ttcctcaatc atcggatctt cttggacatc
      361 cacatactgc actcgtccac ttgggctcgg agacttcttg acttcttcga taaatttttg
      421 gatgatgtcg tcatttccga ttgcttcgat tgtgactgag ccgtcgtctt cgttttttac
      481 cgttccggtc acgcctaact gatcagcaac catcttggtc atatagcgaa atcccacgcc
      541 ctggacacgt ccttggacat tcattttaac tttgcgcatg ttcgtcacct ttctttcctg
      601 ttttataaat caatgatacc gattttttta ggatgaaaca agctcaagca cctattccat
      661 gatataatca gttgaggtga ggaatgtgaa agagatccaa tcagtaaaaa atccaagaat
      721 caaagaatta aaaaaactac ataaaaagaa atacagagaa gaaaaggcag aatatctttt
      781 ggaagggttc catctgatcg aagaagctgc gtcgtctgat gcagaaataa aagaactttt
      841 tgtgacacag agaggtatga aagaatgggg agactggctg gctgtaaaca aagagatttc
      901 tacttttcta gtttcagatg aggtcatgac cgctatgtca gatctaccta caccgcaagg
      961 gatcttagca gtgatgacgt taacatctga agatacgcca gatttttcag gaggctggct
     1021 tttactagat aatgtacagg atcctggaaa tgtgggaacg atgatccgta cggcagatgc
     1081 gtttggtatg gctggtgttt tattaggtaa aggaacggca gatatttata gtacaaaggt
     1141 gctgcgaagc atgcagggaa gcaattatca tttgcctgtg ttacaaaaag atttggcaga
     1201 agtgatccct gtaatgaaag gcaaagggtt tacggtgtac ggtacagaat taaatgaaga
     1261 agcacatgca ttagacaaag tagaaaaaac agaggatttc gctataatca tgggaaacga
     1321 aggtcaggga gtaagtcagg aaatcttgtc ttcggctgat cagaacgtat atatcccaat
     1381 gaaaggaaat gcggaatcat tgaatgtcgg agtcgcagca gggatcgttc tttatcattt
     1441 ttctttatga ttaaggaact attaagcttt agctatttta tctagagcga ctagaataat
     1501 ccgaacactg gtggtatact tagatcaatt tcagaagaaa atgaaattca aaaaggtggc
     1561 agatcgataa ttatgacaga aaaatggcgt gaagacgaag aatatatgtc ttatgtacaa
     1621 gatcttttag aaacagaagc agtgaaaaaa ttagcgaatt acacacagca tgttcattcc
     1681 actcgtttgg agcattccat cagtgtttct tactatagtt atctgctcgc aaaaaaatgg
     1741 ggtggcaatg ctaaagcaac agcgcgtgct ggacttttgc atgatctgtt ctactatgat
     1801 tggcgtacga cgaaatttga cgaggggaca catgcgtata tccatccaag aattgctgta
     1861 aagaatgcag aaaaaatcac cgatttgtcg gatcttgagc gagatatcat tttgaagcac
     1921 atgtggggtg caacgatcgc accgcctaag tataaagaag gatatattgt gacattcgtg
     1981 gacaaatact gcgcagtcaa agaagcggct cagcccatgt ctgcaagtat gcgtaagaga
     2041 tggcaacgtt actttgggaa agaatcctcg atttaacagg taaaaccaat aatagaccat
     2101 at
//
LOCUS       DACEPW010000301         2088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.308, whole genome shotgun sequence.
ACCESSION   DACEPW010000301 DACEPW010000000
VERSION     DACEPW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.308"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..1515)
                     /gene="gndA"
                     /locus_tag="IW819_002750"
     CDS             complement(94..1515)
                     /gene="gndA"
                     /locus_tag="IW819_002750"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1245828.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            1742..>2088
                     /locus_tag="IW819_002751"
     CDS             1742..>2088
                     /locus_tag="IW819_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1245829.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHV"
BASE COUNT          582 a          441 c          392 g          673 t
ORIGIN      
        1 attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt cgtcctcttt
       61 ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc atgagtaatg
      121 gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc
      181 ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag cgatggctga
      241 tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt ctctgactga
      301 ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat tttcgatctc
      361 aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc
      421 agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc
      481 gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat acaacgcttc
      541 tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga
      601 caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc gggcaaatac
      661 agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc ttgtccattt
      721 acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt tacctgttcc
      781 ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc tgtctaattc
      841 gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt ttaaaatatt
      901 cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa taccgttgtg
      961 taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac atggtgctcc
     1021 gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat aggcttcttt
     1081 ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac cggaaacgcc
     1141 tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca tcgtatcttt
     1201 aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag gaagtaactc
     1261 ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc gacgtggttt
     1321 ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt tatctgggtg
     1381 ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca ctgaatagcc
     1441 gcggctttct atgttcaagg ctagattttt acccataacg gccataccga caacgccgat
     1501 ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat ctcttacatt
     1561 atagcttaac acggctacat tgcaaactaa agaacaacat attttggctc tataatttat
     1621 gggactgatg aatcagaatt gattcttcag ccccattttt attttaggta ttaaaaaaca
     1681 tatcgttctc tagtatgatt aaatcacgac aaaataaaca actgagagga cgatatgcta
     1741 tatgaatgat tctatcaaaa aaatgctgcg attaatagag aaagatttga tgattacaga
     1801 ggtctcttac gagacctttc agaagaaaaa gacgttgatc gtcgatgctg ttttctcacc
     1861 tgctccccat acctgtagaa attgtggttc tactgtggta gatggaaacg ggaaagtaat
     1921 tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt gaacaataca atcacatgcc
     1981 tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa aactgtcgaa cccattggac
     2041 ggctcaaagt tattttgttc aacccagaca ttcaatcgcg aatcatgt
//
LOCUS       DACEPW010000302         2047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.309, whole genome shotgun sequence.
ACCESSION   DACEPW010000302 DACEPW010000000
VERSION     DACEPW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.309"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..154)
                     /locus_tag="IW819_002752"
     CDS             complement(<1..154)
                     /locus_tag="IW819_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1245830.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALD"
     gene            468..890
                     /locus_tag="IW819_002753"
     CDS             468..890
                     /locus_tag="IW819_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245831.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            900..1103
                     /locus_tag="IW819_002754"
     CDS             900..1103
                     /locus_tag="IW819_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245832.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1314..1898
                     /locus_tag="IW819_002755"
     CDS             1314..1898
                     /locus_tag="IW819_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1245833.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          710 a          299 c          358 g          680 t
ORIGIN      
        1 aatctaatgc ttcttggaaa acaggtcgat gagtaatcgt tgcgccagat tgtttctcga
       61 tgaaaattct ctctgcaccg aattttttca tttcttcaat ctggcgttgt tcattttgat
      121 cgatcgatga gacacgtatg tatgcaactc tcaaaacttt tacctacttt ctgcaactat
      181 ttaaaataca ccctaatcat acacgaggaa gccatatttt tcaatgttat accagtgtct
      241 atttagggta aaccttatat agacaaaaac taatgatgga ggaaatagta tgtgtcactt
      301 ttctttttaa atggcaatat tttttgtaaa taaatgtcaa tcaagaaaat ttaaacgatt
      361 atgacattaa aaatatattt tatgaatttt ctagtatata acttgattta tctgttaaga
      421 tttattaaga aaacgttttc ttatttttga tttcgaggtg atagacaatg aaaaataagt
      481 ttattttatc tttaggtatt ttaactattg gaattagtgt aactggaact acaattccta
      541 catttgctga tagtacaaat gaaccaacta ctgtagtaga taataaccaa gaattaactc
      601 ctttagatag gaacggaata gccattggta acccaactaa cgacttaact gttactgaaa
      661 attatttaat agtttctcca ttacttgctc gagcagcttc taaaacgata cgagtgacag
      721 gaacgatgat ttttgataaa gttggttggc cttctagacc tcctcaatca cggaattgga
      781 aagaaaaaag aaatggttat tggtactatg gaaaattgta tttacaaagt tataaatcag
      841 ctggaaacaa ttggattgcg acttattcag gaaccttaac tagaaagtag ggatttttta
      901 tgccagaatt tgtgaacaca ccaaattttt atcaagatag cgatattcta agtaaaatta
      961 atttaccgaa agaagaatca gctttacaag ttcagcgaac tttacaggag acacaaaaaa
     1021 ttcaaaaatc aatttctaat ctagaagatt ctgaagaaaa aagaaaaaaa agagaccaaa
     1081 aaacatatca aaaagaaaaa taatgatttt aaaaaaagag tgtctagtcg acattctttt
     1141 tttctaattg aaagtgttca taaaatcacc catttctgga cactttcttt ttttgcttta
     1201 tttttcaaaa aaacaattca tttcatgttc ataaacccat aacaatatct atgttcagtt
     1261 aaactatatg gataaagtta tgttacaata aaattaatga aggagtgatt tttttgaaaa
     1321 taggatacgc ccgggtatct actggtttac aaaatttgga tttacaagaa gacagcctaa
     1381 ataaatttgg ttgtgaaaaa atttttactg atcatatgag tggggcaaaa agtaatcgac
     1441 caggattaga aatggccatt gattttgttc gctctggcga tacacttgtc gtttggcgct
     1501 tagatcgttt aggtagaaat atggaagatt tgatttcaat cgtcaatcga ctgaatgagc
     1561 gtggtgtgag ttttcatagt attcaagaaa atattacgat ggacaaatca agttctacag
     1621 gacagctcat gtttcatttg tttgcggcat ttgcagaatt tgagcgtaat ttgatcctgg
     1681 aacgatccgc agcaggacga gcagctgctc gtgcaagagg acggtttggt ggtcgtcctg
     1741 aaaaattgtc tgaacaagat ttggaattac tgaagacttt agtggatagc ggtacaccta
     1801 ttaagactat tgcggaacgg tggaacgtct caagaaccac catttatcgt tatttggcaa
     1861 aaatggagga gaaaaatgga aagacaaatt ctcattagtt tttgtcattt aatgctgaaa
     1921 atttttgcca tttatctaga aaagtgacaa tagtatgaga attttttgta agtgtctact
     1981 gataacttat tctcaaatat ttagctattt cttctttaca acatcgaaca tatgtttgta
     2041 taatgtg
//
LOCUS       DACEPW010000303         2046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.310, whole genome shotgun sequence.
ACCESSION   DACEPW010000303 DACEPW010000000
VERSION     DACEPW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.310"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            111..1481
                     /locus_tag="IW819_002756"
     CDS             111..1481
                     /locus_tag="IW819_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAQ1245834.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1502..1786
                     /locus_tag="IW819_002757"
     CDS             1502..1786
                     /locus_tag="IW819_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245835.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          711 a          295 c          437 g          603 t
ORIGIN      
        1 gtattgttta tattaaatta gatgaccgtg ataatgcaca gcaaattttt gaaagtatca
       61 attctaccgg tgaaagattg actgcctcgg atttgattcg aaacttcatc atgatggata
      121 aatctaatga agagcagact acgctttata ctaaatattg gcgtaggttg gaagaagtct
      181 ttgataattc taaaggaatg gaagactttt tcagatacta ccttgctgca ataactgggg
      241 agtattcagc aaagcatatt ctttatcaag cattcaaaga ctattggcat aaagagcgcg
      301 atgtatcaag tgatgccgaa ttgctccaaa aattagtgcg atatgctggt tattttgcga
      361 gactttacta taacaaacct gaaggaaaat actcagaagt attgagcgat ttccaaagta
      421 tggaaagtat gatgcctgca ccttttgttt tgggattatc tgaatggtac tatcatgacc
      481 aatttatcac tgaagaccaa tatattgatg cgattaaggt agtgaatgat tatcagctta
      541 ggagatattt taatggggat gatacaagca gagtgtcaaa agcatttcct tcatatttga
      601 aaagtgttag gaagtacgct aaagctaatg gatatgaaaa tatcgttgat attgtaattt
      661 atgttcttgt tactagaaat caatcaaata atatggcact tccatctgac aaggcattga
      721 aatctaatct attgaatgcc aatgcttatg cgatgagatt ggcccgttgg ttacttgaaa
      781 aaatagagaa tcgagaaaat tcagcaacac ttgatatgag taacttgtcc attgaacaca
      841 tcatgccaca gactagtact tcatattggg aagagaaggc tggtacatcg ggggaagaat
      901 acacaggatt agtaaatacc attggtaatt taacactcgt tacaaatcct gataattcgg
      961 ccgcgggaaa taaagacttt gagaccaaaa agaaaatctt tgaagatacc cttcatattc
     1021 gtatgaataa agatttgtac gaattgactg agtggacatc atcggatatt tctgccagaa
     1081 gtgaagctct aatcaatgaa ctgattacta tgtatcccta tttgagatcg tcgggcgact
     1141 atgaacacga tgggaatagg gaaattttct tagaggcgca aggaataaaa gcaactgggt
     1201 atttgaacga agacgagacc gtgattatac attcaggttc agaaatttat tctaagatta
     1261 aagatattgc atctgattcg ctagatgaaa ctcgccaaga gctgttggat aatggaatca
     1321 ttgaagaaac aattggggga ctacaatttg ttcaagatta cactgcctca tctgtttcga
     1381 atgcagcagc tctgctatta gggggtagcc ggaatggttg ggactactgg aaatatgata
     1441 acggaataag tattaatgat tcgctaagaa ataagaaata gagcatagag gtggtgttag
     1501 gttggataag aacaaactaa gatatgcaat tcttaaattg cgtgatgaaa aaaagaatcc
     1561 ttttattgag cttaaagggc aagttccaga agaagatatt ttagaacaaa ctaaatttct
     1621 ttcgcgcaat ggcttactag aatccatggt ttgggcggat aacactgttc atatctgggg
     1681 gagtgtttct ctggaagggg aaaaatattt agttgaaaac tcaggtcttg ctaaggcgta
     1741 tgcattggca aaagaagtta aaaattggat tccattttgg ggctaagata agtttttaaa
     1801 cggagaaggt agcaaatcat aattggtttg ttgccttttt tgattgccca aaaagaaaaa
     1861 tgacaaagta tagagataaa tgacatagta cacgggaaaa atgacattgt atcaccgaca
     1921 acgtctaaat agatgttgat atatatacgt tccgtacaaa taattataaa aatttaaatg
     1981 atttgagtag aagtatcagt gaatatctca aggggaatag ctatctcaag attcgatttt
     2041 cacata
//
LOCUS       DACEPW010000304         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.311, whole genome shotgun sequence.
ACCESSION   DACEPW010000304 DACEPW010000000
VERSION     DACEPW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.311"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW819_002758"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW819_002758"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1245836.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IW819_002759"
     CDS             complement(1431..1823)
                     /locus_tag="IW819_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1245837.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACEPW010000305         2002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.312, whole genome shotgun sequence.
ACCESSION   DACEPW010000305 DACEPW010000000
VERSION     DACEPW010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.312"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            196..>2002
                     /locus_tag="IW819_002760"
     CDS             196..>2002
                     /locus_tag="IW819_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1245838.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGI"
BASE COUNT          710 a          300 c          387 g          605 t
ORIGIN      
        1 aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt taacagtaca
       61 tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta taagttgcac
      121 ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat attagcaatg
      181 taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt gatttacttg
      241 gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa tgttagtgtt
      301 cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg gaagcaagtc
      361 ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga tcgaatcaaa
      421 ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca aagtctccga
      481 atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg aatgaaaatg
      541 gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt gctggaaaat
      601 aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt ttggatcata
      661 ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga gttcacggat
      721 tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga atactttgaa
      781 atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat cattcgaatg
      841 cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt actaaatatt
      901 ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat tggtcgttat
      961 caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt catgcagcag
     1021 gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat ctatagtgat
     1081 acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt gatttataaa
     1141 gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac gaatctgtat
     1201 gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata taatcctttt
     1261 aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca agcagttcga
     1321 caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata tattgcctta
     1381 cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc cgtctacatt
     1441 tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga agaattaaat
     1501 aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat tgctaccaaa
     1561 gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc tttcatcaaa
     1621 gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat aaaaaaaata
     1681 cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac gtccatcaat
     1741 aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac gatttatgaa
     1801 cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc ttttttgctt
     1861 catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc agaaggtcgt
     1921 caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga agcagttttt
     1981 gcagaaaata atttaggaat ca
//
LOCUS       DACEPW010000306         2002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.313, whole genome shotgun sequence.
ACCESSION   DACEPW010000306 DACEPW010000000
VERSION     DACEPW010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.313"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            229..474
                     /locus_tag="IW819_002761"
     CDS             229..474
                     /locus_tag="IW819_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1245839.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            571..915
                     /locus_tag="IW819_002762"
     CDS             571..915
                     /locus_tag="IW819_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245840.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
BASE COUNT          609 a          367 c          311 g          715 t
ORIGIN      
        1 ttagctattt tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac
       61 tttgaacccg atagtgtttt tatagaagaa ttatcaggac tggttttgtc tttattgatc
      121 gtcattcttt atttgatacg tttaaataaa aaacattgag tactattgat tgcatgtgta
      181 gctttctata gtcattataa ctattctact ataatgaaag gtgattaaat gagtatcgaa
      241 gaagctattt tactggatac tttacgttgt cgtctgaatt tcttaagaac tggccgatct
      301 ttatctcaag tagctgtttc tagcgcgtta agtattcctt tattcaaata tcgtaagtac
      361 gaagaaggag aagaaattcc aagcatcatt gaattaatct atatcgctga cttttacgat
      421 atttccgttg attacctaat tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt
      481 ccagccttgg tgctggtttt ttcatttaac gtctaaggtt aacaatctat atataaagga
      541 gacatttcta tgtgattatg gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt
      601 ttaggacaac atcgggaatg ctgtgcatta agaggaaatg gctggggaaa gaaacatgct
      661 actgtggatt atgtttttca ctattctcct tatagattat tccaatatca tcagcttgtc
      721 atggatgaaa tggaaaaacg tggttacagc ccagcaccag agtggaaaaa tcctatgtat
      781 cgtggcaaat catgcgaagc attttttgat ttacctactg tagagcgcaa cgtgccgatt
      841 tatccggaac acgatgaagt ttatctagca gaatgtttag caaatttgga gcaaaaagga
      901 atttatctta cctaatataa ctagaaaagc cttctatctc ctcttacttc attaaaaagc
      961 tttaaccatc ttagatcaag ctaaaatgtc gtaagtttta gggttatctt catcctcacg
     1021 tgcaaaacta aaagcgtact ctagttttgg ttcatcgcca acttccctag acataaatga
     1081 acatctcctg acgtggttta aaagtaccgc atcttcgacg tctgtatacg atgcacagca
     1141 cagattgctt cttttgatct ttcttttgat ttttctgttg cttcttctac accttcttcg
     1201 attactacat cgatgcttac aattgcttct gcctaatatt cccatgcatc gtcgatttaa
     1261 gcctctgtag tcactgtgat cgtttccaca ttttaactcc tctgtcgtgg cttcagttga
     1321 ttttgtgatc agttcagttg ctaagccaga gtcagaacaa cctgccattg ctagcgttaa
     1381 tattgatagt aaaaatagtt tttttatcat aaggttcccc cccctgaaaa tagactaaaa
     1441 taataacgct ttcttttttt gttttcagac gaaaattaat aatttctaaa tattctttaa
     1501 aaaagcttat aactattatg ccttttttaa tatataacac agttattttt aaaaataaga
     1561 atgctaaatc actctacaat aacggcgaaa gcacaaaaaa taaaaaaatt attacgttat
     1621 taaaaaaaca gatgaaagat caggttttat tagatctcat tctttcatca cttgaaggga
     1681 tttaatgaac tactttttcc tctttgtcgt ttttatatat tcgtattcct tcaaacagta
     1741 ggatagcctc tttcaatgct tgatatttga actgtccgtt acttaaaaat ttagatgtgc
     1801 cgccctcttc ctagaacata ttctttttta ctttttcatt aattgacaga ccaatcgaac
     1861 gaaactgtaa atttcgatag gataatgatt ggtcaaactc attcattttc aagacatctc
     1921 gattcatatt caagatagga atctttttat caacatcact ttattcgtat tttcctctac
     1981 cataaaagct tcacgctcca ca
//
LOCUS       DACEPW010000307         1988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.315, whole genome shotgun sequence.
ACCESSION   DACEPW010000307 DACEPW010000000
VERSION     DACEPW010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.315"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..221)
                     /locus_tag="IW819_002763"
     CDS             complement(<1..221)
                     /locus_tag="IW819_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAQ1245841.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQ"
     gene            945..1931
                     /locus_tag="IW819_002764"
     CDS             945..1931
                     /locus_tag="IW819_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1245842.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT          715 a          293 c          384 g          596 t
ORIGIN      
        1 tcttgttttt ctaaatggat aatcagctga tcatccactt cttcagaaaa tacttttctc
       61 aaataatcag taaacagatg aaaatctttt gcgtattcat agcgactgcc taatgaaagg
      121 gaagaacgat tttcttctaa aaactgttga aagacggaag ggagcaagtg tgtacgttgc
      181 tttaactttt cttgcagttg ttttctagtt ttgctgtcca ttacaaattc cctccaatag
      241 tgttcatttt ctatatttta ccgcatttca tcaaatgaag atagacagtt tgtcttgatc
      301 ttaggaagca aatttttaac taagtactag ctaaaagata tgaagtaaga agatacattt
      361 ttagttattt tattagtgca gtcattcttt tgatctttta attgatttat cctcttttta
      421 gctatgtggt tacttcatga gcaatgaaat ggatttggtt cgttctaatg gcaggaggct
      481 ctttctttta tttccgaaaa attagaaaac attttttcag aatcgattgg ctctccattt
      541 attcaataat ggtttgtcta acgatattgt tgggataatt cttaaagaag tattagaaat
      601 agttggtact tcattacttt acatgaagat ttattctatg gtaggagcaa ttatattgag
      661 aattaatcct tttttatata agttgtttgc ttattgatgg attggataaa ttaaaccatc
      721 acaacgagtg gaaattatat gaaatttttc ataaaaattc atatagaaac aaatgacatt
      781 ttattagaat atgactttca ttaacataaa tgttttcaaa tttcaattaa attgttttaa
      841 tcaaattcca atctaaatat ttgtagaaaa caatcgtctc ctatagaata aatttataag
      901 taacaatata ccaatcataa atctatttgc taggaggaat ttacatggaa gagaaaaatt
      961 taacggtcat cggaagctat agtacgaggg aagaagcatt atcagtaatt gaacggttgc
     1021 gtaacgaagg gtatgaacga gatgatatcg ttatttatac tactgatgaa gcagcatcta
     1081 gactaggatt tgacggattg tctggtatag atgttgaaac agatgaaaat cggatggatg
     1141 gtgaagaaga ccgttcttta tgggaaaaaa taaaagatac tttttctttt gatacctatg
     1201 attctgagac ggctacacca gaaaatgatc ctttatatca atatagagga gatatttctg
     1261 atggaaaatt tgtcatcaca gtaaaaggct atcgtcagcc agaaacaaca gaagatacaa
     1321 tggatacaca agatactgtt agcccagcaa gcacaatggg gactggatta gaaactggca
     1381 ctggaacaga tccaatggac gtaggtggca cgacaggctt tcaaaataca acggatccat
     1441 tgaatatgac accaggaact gatccgatgg acgtacctgg cgagactgat acaatgggta
     1501 caagcaagac tgaagacatc gatcgcaaac caaatctaga tgatgataca attcaactaa
     1561 aagaagaaaa attagatgta aatacacatg atgtgacaac tggagaagtc gacatccaca
     1621 aacatgtggt caacgatact gaaacggtag aagtaccggt caaacgtgaa gaaatcgtca
     1681 tcgaacgcaa accagtaaca gatcaatctt cacagggaac agacgaaaaa ttggaagatg
     1741 acacgattac tatcccaatc aaagaagagc aagtagatgt aagcaaacat acagttattc
     1801 gtgaagaagt tgggattcat aaagaagaac atgaagatgt tgaaaaagtg acagaagatg
     1861 ttagccgaga agaattggat attgacacaa gcggtgatgt acacattgaa gaccgcaata
     1921 aaaaatcgta agcgtaagct aaagaggttg tgacagaagt ggtcagctta aagaaatttc
     1981 ggcttatt
//
LOCUS       DACEPW010000308         1953 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.316, whole genome shotgun sequence.
ACCESSION   DACEPW010000308 DACEPW010000000
VERSION     DACEPW010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.316"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            153..1313
                     /locus_tag="IW819_002765"
     CDS             153..1313
                     /locus_tag="IW819_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1245843.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTIAEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1329..1772
                     /locus_tag="IW819_002766"
     CDS             1329..1772
                     /locus_tag="IW819_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1245844.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT          683 a          312 c          388 g          570 t
ORIGIN      
        1 aacaaaaaat atgagtagct attttttgag gctgtactta ttacttgatg caggacagct
       61 ttttcacaat ctcatcggct ttcattttct atttttttcc tgaacccata taataaaaga
      121 aagaacaaaa aaacttgcaa gggaggacac atgtgaagca gagtaagcaa aaacagtctc
      181 gtcgactttt gtttcgtttg tttttattaa tatgtatcgt cgtagggtta ctgatcgctt
      241 tatatccatt ttatgtggat tctttaaata gttttattga tcaaaaacgt atcgaacaag
      301 tacaaaaacg gaccgcagct gaaattgaag tgcagcgaaa gaaaatggag gagaaaaacc
      361 aacgattaac tgaccaagga ttcaatcctg gtactgatcc tttcaatgaa aagaacagaa
      421 atgaaagtac aacatccagc caattagagg aatggctgat cggaagtatc aatataccta
      481 agatccaatt aaatatttca ctttatgatc ggctcaatgg taggatcctt gaaaatggag
      541 caggcgtctt gcaaggaacc tctttcccgc ttggtggaaa ttcgacgcat tcagtgatct
      601 ctgctcacag cggtttacct aatcgacggt tatttaccga attagatcgt ttagaaaacg
      661 gagacacgtt tattttgact gttttgggag agaaattggc ttatcaagtt aaaaatatac
      721 aagtggtact gccagatgac acgtctgttc taacgattgc agaaggagaa gacttagtta
      781 ccttgcttac atgtacgccg tatatgatca acacgcatcg tttgctagtg acaggacatc
      841 ggattcctta taacgaatcg gtaaaaaaag aagaaggaaa aggaaatcag gaaagaacca
      901 tgcggcaatg gtttattctt gcaggaacga taatagctgt agtcatactg ttagtgttta
      961 tcggacgact aatctatcag tatagattaa gtaaaaaagt gcttgatttt tcatttatta
     1021 ttagtgattc gtcagggaaa ccggtaaatg gtagttcatt cattttgaaa cataagaaaa
     1081 aaacacttac tagaaatggt gtgccgtttt cagtacaaag cgaccatcat gggaaagtca
     1141 agctggatca attgcctggt gggacttatc gaatcgtttc agctgatgat cctaaagttg
     1201 ctgtttcttt tggtattaga aaagtaaaac aagagaagat atactttttt gaggggcgaa
     1261 aacttgtaaa agagcttcaa aaaaatggtt tttattttaa aatcaatgaa taagtaagga
     1321 gagaaaaaat gatccgcaca acaacaaaat tgtctgccaa agaactggaa tctgttctat
     1381 cgatttggct acatgctaac ttggattctc atgaattcat tccatcaact tattgggaga
     1441 atcaagtaga atctgttagg aaagctttac cagatgcagt gatttactta gcagaaatag
     1501 atcaagaaat tattggtttt gctggggtga ttgatcagtt tatcgcagga atctttgtga
     1561 aaagggaata ccggcaacaa agaatcggta cgcaactaat gaaaaaaata aaagaaacca
     1621 ataaaacact gactttgaca gtctatcaaa aaaatcaatc agctattcat ttctatcttt
     1681 cacaaggttt tacaattcaa acacagcaaa aagatgaagc agtaaatgag gaggaacttg
     1741 tgatgaaatg gaatcaaaac gctgattttt gatgaacaga ataacgaaat aatgctccca
     1801 gcctaatttt ttgctgggag cattatttca ttttgaagtc actaatttaa ctgtgtgaat
     1861 atgcagttgt tacaatcttt gataaacggt gttcaaaagc ttgcatctac ggtaataagc
     1921 ccaaccaaac aaaaaatatg aggagctatt ttt
//
LOCUS       DACEPW010000309         1911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.317, whole genome shotgun sequence.
ACCESSION   DACEPW010000309 DACEPW010000000
VERSION     DACEPW010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.317"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            232..798
                     /gene="amaP"
                     /locus_tag="IW819_002767"
     CDS             232..798
                     /gene="amaP"
                     /locus_tag="IW819_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1245845.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            811..999
                     /locus_tag="IW819_002768"
     CDS             811..999
                     /locus_tag="IW819_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1245846.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1012..1569
                     /locus_tag="IW819_002769"
     CDS             1012..1569
                     /locus_tag="IW819_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1245847.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1601..1843
                     /locus_tag="IW819_002770"
     CDS             1601..1843
                     /locus_tag="IW819_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1245848.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          697 a          300 c          399 g          515 t
ORIGIN      
        1 caatttttaa tcttgaataa tatcctttaa gtatagccag caatcaaaag taaatagaga
       61 ataagtaagc aattgttatt tcagtatcag actggcaata aaacgccgtt atgatccagc
      121 aatgtttggt agcgctttat ttactgatgc tatgctaact gttcgctatc tatactgtga
      181 ttgtcggtag agttatttac agggtaaaaa taagtttata ggaggtggaa aatgaataag
      241 gggttaaaaa taatcggtgt tatcatgagt ttactgctac tttcagtatt tctagcgatt
      301 gcgttgatca atgcgccgta tattatgcct gaacaattgg aacgatttcg cttctttact
      361 ttaacgaatt actacatgca acaatatatt ttctgggcag ctgtagtatt tgccgttctt
      421 gctattatca tcttgctagt cgttcttttc tatccaaagt caagaggaac tttcgttatg
      481 aaacgggaag atggcaaact aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc
      541 catttacacg aagaagagtt cattcattca cctaaagtac ggatacgttc aacgaaaaat
      601 cgaatccata tccatgtcaa cggagacttg aaacgaactt cttcattagt agggaaaaca
      661 ggtgtattga tgcaagatat cgaagaagaa gtaactaaag tactcggaac aaaagaaacg
      721 gtcaaagtgg cagtcacata cagcggttat gaagaacaag aagatacaag agactacaag
      781 cactctcgag tggaatagga gggccaaata atgaaagaac tattttcagc atacaagctc
      841 cctattatct ttgggggtct tggactactg ctagctgttt tatggttaac gattggattt
      901 ttcaagacat tgttgatcat aatcatgaca gcaattggcg caggaatcgg tttttatttg
      961 aaacagtcag gaatagttga tcaatttttt aaatcatagg aggaaacaaa gatggaaaac
     1021 aaaaacatga atacaacaaa tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg
     1081 atgaatggtg aattgactta tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg
     1141 aaagatgtag atggtctatt gacattagat ggcggatttt tctcaaatat ggctgaaaaa
     1201 ttagtcaaca cagataacgt aacagcaggt atcaatacag aagtaggtaa gaaacaagta
     1261 gctgtggatc tagatgtgat cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa
     1321 atcaaagaat tgatcagtac tgaagtaaac aatatgacac atttagatgt tatcgaagtg
     1381 aatgtgaatg ttgcagacat caaaacacaa gaagaatacc aaaaagatca agaaacagta
     1441 caagacaaag taacagaagc agcaaaatcc acaggacaat ttgcttcacg ccaaacagat
     1501 aaagcaaaat ctgctgtagg caaaggtgca caaaaagtca aagaaaataa cgaaccacgt
     1561 gtacaataag aagtaagaag tcagttagga ggaatttaaa atgggattta tttgggcatt
     1621 aatcgtcggc ggtatcattg gagcaattgc aggagcaatc acgaataaag gatcatcaat
     1681 ggggatcatc gcaaacatca ttgctggttt agtaggttca gcaattggac aagctttact
     1741 aggaacttgg ggaccaacaa ttgcaggaat ggctatcatt ccatcattga tcggtgcaat
     1801 cattttagta ttagtcgttt cattctttgc acgtaaatta taagcaaatt gaagaaaaac
     1861 ttatgttgaa atgaagaaca aatttgagaa ccgtagcgac actcgctgcg g
//
LOCUS       DACEPW010000310         1891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.318, whole genome shotgun sequence.
ACCESSION   DACEPW010000310 DACEPW010000000
VERSION     DACEPW010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.318"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            323..919
                     /locus_tag="IW819_002771"
     CDS             323..919
                     /locus_tag="IW819_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1245849.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(977..1459)
                     /locus_tag="IW819_002772"
     CDS             complement(977..1459)
                     /locus_tag="IW819_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245850.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1565..1762)
                     /locus_tag="IW819_002773"
     CDS             complement(1565..1762)
                     /locus_tag="IW819_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245851.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          654 a          368 c          282 g          587 t
ORIGIN      
        1 aaaaaactta tttaagaaat ataaactaat tttctataaa agcaagtaat ttttaatggc
       61 aatatcataa aaaaaagaag atagagattt aataattata tatcagctgt ttttttcaaa
      121 taagcaaaaa ttcctaaaac acaacaatga aagcgataac taagaaaatc cttacataac
      181 gatttttctt attaaaattg ttttttgaac ttcgcttatt ttttgtgagc atatctgttt
      241 acttactttt tgtaaggtgc tatacttcat ttcgtaatca attaaaaaaa tgcaaaacgc
      301 aaatcaaagg agaaatttta ttatgacaac ttttactgac actttgaaaa accgccgttc
      361 aatctatcat ctaggacgta atgtttcact ttctaatgaa gaattaacaa ctttgatcaa
      421 agaagcaatt aaagaaagcc caactgcttt caatgctcaa tcaacacgcg cagttatcct
      481 tttcggagat gcacatgaaa aactttggga aatcactgaa gaagcacttc gcccattgac
      541 tccagcagaa gctttcccaa atacacaaaa caaattagct ggtttcaaaa atggttatgg
      601 tacagtatta ttcttcaaag atactgacgt ggtaaaaggt ctacaagaac aatttgaatt
      661 atatgcagac aacttcccag attggtcaga acaatcaaac ggaatcgcaa cagctaatac
      721 ttgggtagca ttagttgata aaggattggg cgcaaacttg caacattaca acccagttat
      781 tgacgaagca gttgcaaaag aatggaatat cccttcaaac tggaaactac gttcgcaatt
      841 agtattcggt tcaccagaaa caccggctgg ggaaaaagaa tacatgaacg atgccgatcg
      901 cttccgcgta tttggataat tttatttgaa ataaaaaaca gaggttgtga caaacatctt
      961 gtcgcaacct ctgtttttat tctggatcaa tcataatatc aatctctgcc tcatcgttta
     1021 agcgttggga caaaaatgga actaattctt ctaatgatgc agaaaatttc agcatcggtc
     1081 ctcgccatcc gtttcggaaa aaattcattt ttacgatttt agaacctttt ggctgatata
     1141 atacaatttt attgatctca gaaaaaggaa cacctttttt gtcaaagcta ttcaaaacaa
     1201 gtccttcttc tgtgattcct ctactgtcca acaaaaaact aataaaaatc attgctgaca
     1261 agatcccttt tgccaattca tctgctgtag gatgactgat gagtgtatac caaatgacta
     1321 gtgacactaa gaagggtatc accaagtaga cccatcgatt tcgcacagtc cgataaataa
     1381 tttcttttcg aaccagccaa agaaataaaa acatcacaac aacgaataaa aacaatagaa
     1441 ccgtaaagaa tgttaccatg acaactcgcc tcgctttttc ttctccagaa aataggaaaa
     1501 gaatactgat ttaccttctc aaagaaattg gacttgcagc acgccgcacg tccaattgtt
     1561 ttctttattc aaatgtatca tattttgcaa caaccatgct tgctttttca tctaataatt
     1621 cttggtcctc tgcagcacat tgggcaactt tgaccacggc gcagtttgtc atttttgata
     1681 caacttcacc aatgaccgtt tttgtaaaac caattggttt gcatgcatat tgttctccta
     1741 aattaatcat ttcttccgtc atcaaaatcc tcctaaactg ctggaacttt tctccacatt
     1801 accactgcta tttctttgga gatttaactc attgattttt atttacagtt aaaaatcaac
     1861 cttacaggca ttatagcgta ctgttttcgt t
//
LOCUS       DACEPW010000311         1891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.319, whole genome shotgun sequence.
ACCESSION   DACEPW010000311 DACEPW010000000
VERSION     DACEPW010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.319"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..465
                     /locus_tag="IW819_002774"
     CDS             <1..465
                     /locus_tag="IW819_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1245852.1"
                     /translation="WLNSEAYAPLRTDSIPIGVKENTTGSAFDFQIPKKLASVFASDL
                     DQKNLVDGIDHPFFLKETGLDKEAARLTSPDKRIQVDIATDASSVVIFTANFGTETPE
                     MRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVTEFKIKTRKE"
     gene            472..1659
                     /locus_tag="IW819_002775"
     CDS             472..1659
                     /locus_tag="IW819_002775"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1245853.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELIVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
BASE COUNT          645 a          339 c          391 g          516 t
ORIGIN      
        1 tggctcaata gcgaagctta tgctcctttg cgaacagata gtattcctat aggggtaaaa
       61 gaaaatacta caggaagtgc tttcgatttt caaataccta aaaaattagc gtccgttttt
      121 gctagtgatt tggatcaaaa aaatcttgtt gacggaatcg atcatccatt tttcttaaaa
      181 gaaaccggct tggataaaga agccgctcgc ttgactagcc ccgataaaag aatccaagta
      241 gatattgcta cagatgcttc tagtgtggtt attttcactg caaatttcgg cacagaaaca
      301 ccagaaatgc gcaaccgaaa actggcacat catggtggca tcacatttga gactcaaaca
      361 gctccaggtg cagagcgctt ctcagcattt ggttcgattc atttaaaagc cggatcagta
      421 tttgaaacag tgacagaatt caaaatcaaa acaagaaagg aataactcat catggaaatc
      481 aaaagaagct taaacacaaa atttgccgaa acattcggac ctaaaaaaac tacacaatac
      541 ttctcaccag gacgcatcaa tttgattggt gaacacacag attacaatgg aggccatgtc
      601 ttccccgctt ccatcactta tggaacgcaa ggtgtcgcag cacctcgaga cgataataaa
      661 atattagttt attccacaaa ctttgacgat atcggtgtca ttagttttac tttagatgag
      721 ctagaatatg ataaaaaagc aaactgggca aattacgtaa aaggaatggt ccttaaatta
      781 aaagaagctg gacactcaat cgatcatggg tttgagttga tagtggaagg aacaatccct
      841 aatggtgcag gactttccag tagcgcttca ttagaattat tggtaggtgt tgtattagaa
      901 gatctgtttg atttaaaaac agatcgcttg gaactagtcc aaacaggtaa aaaagtagaa
      961 aatgaattca tcggtgtcaa ttcaggtatc atggatcagt ttgccatcgg cttcggtgag
     1021 ctagatcatg caatcttatt agatacaaat acgctgaaat acgaaatggt cccagtgaaa
     1081 ctagatggtt atgcaatcgt catcatgaat acgaataagc ggcgggaatt ggcagattca
     1141 aaatacaatg aacgccgcag cgaatgcgaa gaagccttgg cacgtttgca aaaaactttg
     1201 cctatctctt ctttaggaga cttagatgaa gaagaatttg aatcaactat tgaccaaatc
     1261 ggcgatgata cattgatacg cagagctcgc catgccgttt ctgaaaatca gcggactttg
     1321 aaggcaaaag ctgaattaga agctgggaac ttagaagcat ttggtcaact gttaaatgat
     1381 tctcaccact ctcttcgcta tgattatgaa gtaactggga tcgaattaga tacactagta
     1441 gatgctgctc aaaaacaaga aggtgtctta ggtgcacgaa tgacaggagc tggatttggt
     1501 ggctgtgcga ttgctcttgt caaagaagaa aatatacctg ccttcaaaaa caatgtctat
     1561 gatgaataca tgaaagttgt cggctatgct ccggaatttt atgttgcaca cattggaaat
     1621 gggacaacaa agatcgatcc agaagctgtt catgtttgat aaaaaaatca acgatctcag
     1681 ctaaaaacaa atagataaaa ataacctctc agcaattttc aaatgtgagc cttttgctcg
     1741 tattcgaaaa ttgctgtttt ttattgacac agaaagagaa catgtgttct aattgtattg
     1801 aagaggtgat tgttatgtgg gcgaatcaaa atccggtatt tgtttatgaa aaagaacctt
     1861 caagagatat tttatgtatt gactgcaaat c
//
LOCUS       DACEPW010000312         1888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.320, whole genome shotgun sequence.
ACCESSION   DACEPW010000312 DACEPW010000000
VERSION     DACEPW010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.320"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..701)
                     /locus_tag="IW819_002776"
     CDS             complement(<1..701)
                     /locus_tag="IW819_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1245854.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIH"
     gene            complement(927..1580)
                     /locus_tag="IW819_002777"
     CDS             complement(927..1580)
                     /locus_tag="IW819_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1245855.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT          567 a          401 c          271 g          649 t
ORIGIN      
        1 ttgtgaataa gttggcgtgt cagccatttc ttcccatctt cactcacttt ttctattttt
       61 ttcatttggt tcattttaat ttcgttacta gtgaatattt ctttaaggtc cctactagaa
      121 aaagtccagc agctactgca aaaggaaata tgccctgata cagtctctgt aatatgatga
      181 tacaaataaa gcttttgttt cttgagatca gctttccagc aatgcactcc tctttttcta
      241 ttatagtagc agcaactttt ttctatcaat gagaattgcg attgtccaag aaaagaatta
      301 ccaagtatcc accatacttg gtaactgtta ttcaaataat tttgggttct ttcaataaat
      361 ctctggtgag acagtctaga acattggatt tctattgcta gattcgccaa caaaatatcc
      421 ggcctttgcc gcaacctcgg caaataggct tccaaaaata ctggttctac tgactgtccc
      481 agccaatcca tgaagcattc ctttaactgt aagtgctctt ttgattctct ttcactaaag
      541 accgaacagt tatctgattt ttgatgggca aaatgagcac attttttctt tccctttttc
      601 aaaatcactc tttttcgaca gccacgacaa taatatttct ttcctttttc tgcatgttta
      661 gcaaaacagc acttattata ttcattcatt gcaagcagca tttttctccc tctgaactta
      721 tttacgtcta agaatttatt tacacgaaaa aagagggcgt gaaaaagcta ttctgcatca
      781 agtaataaga acagctaaac aaaaatagtt cccaatattt ttcgtttggt tgggcttgtt
      841 accgcagatg caagcttttg aacacattta tacgaaaaaa agactgcgac acaatttttg
      901 tcacagtctc ttactataat gctgatttat acaaaataaa atttcgttcg ctcgatagca
      961 ttacgcgcca tcagacattg tccatattca ttcaatgttt ctggtgtacg atcgctcttt
     1021 tcagcaaact ccaaaattcg agcaatctga ttctctacat ccgcttggtt gagattttcc
     1081 atccagaagt gaacgactag gtaatagcgc tcttcgaata aatacaaatc agtccaagca
     1141 gactctagtt cgacatctgt tgcaagttgg atcacttgtt caaaatcttc aaaagcaaaa
     1201 gttgcttgtc tttcctgttc ttctagggcg cttccagata attcttcctc cggatcgctg
     1261 ccaataatct gctgacgaag aaagtcactc acatcttctg aatcaacagt agaatcttct
     1321 gattgagaaa tttcttcatc cggaagattt ttactgataa acagttctaa tccgtctcct
     1381 tttggcaaaa cttggaaagt gacagcttcg cttcctcgga actcctcttc gatatcaact
     1441 tcttccaaaa tactataaaa gaaattttcg atttctttgt gattgccaag taaatccaaa
     1501 aatgaaacgc cccgttcctc taaatcttca tgaccaatta gtacacggat cgtattttca
     1561 ttaatatgtt ccatttccat tttgctacac ctcactttgc cacaatgttt aatatagatt
     1621 cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc tcgctgttaa gcgaatttaa
     1681 taaaaccagc ggatttttca acctttcaag ctatcttatt taactataac atttttggca
     1741 gacgaatact atatagccga cttttcagct aattttccct ctctattttt cgtgcaatca
     1801 tcatcttcta ctaccttttt atacatctaa tcaagaatac tttttgtaaa aacaaaaaaa
     1861 cttggaaaaa ctatctttgt aatagtct
//
LOCUS       DACEPW010000313         1883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.321, whole genome shotgun sequence.
ACCESSION   DACEPW010000313 DACEPW010000000
VERSION     DACEPW010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.321"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..884)
                     /locus_tag="IW819_002778"
     CDS             complement(<1..884)
                     /locus_tag="IW819_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1245856.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELY"
     gene            1114..>1883
                     /locus_tag="IW819_002779"
     CDS             1114..>1883
                     /locus_tag="IW819_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1245857.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLY"
BASE COUNT          537 a          409 c          313 g          624 t
ORIGIN      
        1 ttgtataatt caaacagctc tgcttctgtt tctttcactt gctgcgctct tgtaccaatc
       61 gtttgatatt cttctaaagc atgttccagc atttcttctt cacggtagta atagcggtgg
      121 tagccacaag gaatcatgtt catttgtttt aattgttctt tgaaaaacgg gatatcaaag
      181 atatttttcg gcaatccatt gtcttcatcg tataaacgat caatcaattc tgccgtgcgg
      241 tcatttcctt tattatctgc tactttgtgc caatggaagt gatttagacc ggcaaacttg
      301 tagatcagct cttctttttt cttgccaagc attgttggtt ctgtcatcat cgccattact
      361 ggcacattgc ataatccgat cactttatcc catttgccat atctgataac tgcttctgtc
      421 accatcccgc ttgggttcgc gaaattgatc agccaagcat ttggacataa acgcttcatg
      481 tcttcaacga ttgccaaaac cactggaacg gtacgaaaag ctttgaatat cccgccagca
      541 ccatttgttt cttgcccaat catcccataa taactaggga tccgctcatc tttgattcgt
      601 gcatctaatt gaccgacacg aaactgagtc gtaacaaaat ccgcatcttt caatgcttct
      661 tcccgatcca gagttgcata gattttgact tcatacggag aagcatccca catacgctga
      721 gacatctgac tgacgatctc aagcttttct tttccttctt ctacatctac gagccagatt
      781 tctcgtatcg gtaattcctc atatcttttg ataaatcctt ccatcaactc gggggtgtaa
      841 ctgctgcctc caccaatcgt agcgattttg attccttttg ccatgttctc actcctttac
      901 tttttacact tttattatct tttttgtaaa caaaacaaac aatagaaatt cgtttctttc
      961 cagtaacatt tacaaaagtt agtgttttct tctggatttt tgtaaatatc ttgtcaaaaa
     1021 atgtctttat taagctatat aacataaatc cgtttacaaa tcattttcaa aaaagttata
     1081 atttacgtaa aaaaacaaga agaaggtatc tacatgttat tacgtgaaaa aatcaaagaa
     1141 atctcctttt ctccttcaga aaatgaagtg attcagtttc tcttaagcga ttctccagca
     1201 cttggcgaaa tgaccatcca agagattgct gagcgatgtt atgtccaccc gtccacactc
     1261 atccgtgtag caaaaaaaat gggttttgat ggctgggtca gttttcgtaa tgctttttta
     1321 gctgaaacag cctatcttga agggaatttt caagacgtag atgcgaatct tcctttttcc
     1381 accacagacg gtattatgac gatcgcaaat aaaatggctt ctttagaaca gatgacgatt
     1441 caagatacac tttctctcat tcatcatgac caactgcaaa aagccaaaca actaatgctt
     1501 caagcaaaaa aaatcgtgat tttcgccagt aactcgaata ctttgatttc tcaagatttc
     1561 atgctgaaaa tgaagcgcat caaaatggat gtcacgattg tcaccacttt tggtgaatcg
     1621 ttctatgaag cgtacaattg cacgagtgat atgtgcggga ttctgatttc ttatacgggt
     1681 gaaaacaatg tgatccttcg taccgcagaa atattgaaac aaaatcatgt gccaatttta
     1741 gctttgacca gtatcggtga ttcaacgcta tcttcaatga gtacagccgc tttgcgactt
     1801 acgacacgcg aacgactgta ttcgaaaatt ggtaatttta cgatcaatac atcgatttgt
     1861 tatcttctag acgtgttata tag
//
LOCUS       DACEPW010000314         1876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.322, whole genome shotgun sequence.
ACCESSION   DACEPW010000314 DACEPW010000000
VERSION     DACEPW010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.322"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            27..686
                     /locus_tag="IW819_002780"
     CDS             27..686
                     /locus_tag="IW819_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1245858.1"
                     /translation="MREGNQALSSEELIAWIEAVNTDLNNHLLTLHDYTLFMLTLYLG
                     DRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTVIQVPEIVMALLSKWK
                     SVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPELAHLSP
                     HKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVVDKTVFEAFQRGLNKC
                     D"
     gene            complement(775..1167)
                     /gene="rpsI"
                     /locus_tag="IW819_002781"
     CDS             complement(775..1167)
                     /gene="rpsI"
                     /locus_tag="IW819_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1245859.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1183..1626)
                     /gene="rplM"
                     /locus_tag="IW819_002782"
     CDS             complement(1183..1626)
                     /gene="rplM"
                     /locus_tag="IW819_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1245860.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT          583 a          351 c          347 g          595 t
ORIGIN      
        1 aaaaaattgc tttagaagaa agaaggatac gggaaggtaa tcaagcatta tcgtcagaag
       61 agcttattgc ttggatagaa gcagtaaaca ctgacttgaa taatcacttg cttactcttc
      121 atgactatac tcttttcatg cttaccctat atcttggtga tagaaaaagt gagacgtatg
      181 ctttgcaatg gaaatatatc gactttgaga aacaaacggt tcgccttaaa catgctttgg
      241 ataaatatca aaaaaagaaa tttacaaaag gtcgaaagga taccgtaatt caagtacccg
      301 aaattgtaat ggcattacta agcaaatgga aatcagtgca agctgagcaa ttgttgagat
      361 taaaaattaa tcaaacacca gatcagtatc tctttaccta tactaaacca tccggagaag
      421 taaattgccc tgttcacgct gattacctaa actatcgaat caattcaatt aaaagacgac
      481 atcctgaatt ggctcattta tctccacata agcttcgtca tacctatgca actatagcta
      541 gacaaggcgg agcaaatatg aatcaaatat ctagtgcttt aacacactca gatatttcta
      601 caacgagaat atacgtaaat acaccagatg tagtagataa aactgttttt gaagcctttc
      661 agagaggatt gaataaatgt gattagtatg tgttcttaaa tgtgttctta aacaagaaaa
      721 agacactttt caaacaattg aaaagtgcct tgaaacattg atatataaat aatattaacg
      781 tttagagaat tgagatgctt tacgagcctt tttgagacct ggttttttac gttcgaccat
      841 acgtgcgtca cgagtaagta agccagcacg tttaagagct aaacggaagt caggatcaac
      901 ttctagcaat gcacgagcga taccatgacg gatagctcct gattgtccag cgtatcctcc
      961 gccgtttacg ttaacaaata cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg
     1021 attgattact tcacgcaagt cagcatgtgg aatgtattct tcaacatctt ttttatttac
     1081 agtaattttg ccagttcctg gtactagacg tacgcgagca actgcgtttt tacgacggcc
     1141 tgtgccgata tattgaactt gtgccaatga aattccctcc tattagatta agtttgtgat
     1201 gtctaaaact tctggttgtt gtgctgcatg tggatgttct gctccaccgt atacgttcaa
     1261 ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc atacctttta cagaagtttc
     1321 gatcaaacga cgagaatttt tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc
     1381 tgggtagttg ctgtgacggt aataaatttt gtcagtcgct tttttacctg ttaatttcac
     1441 tttgtctgca ttgatgacga tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg
     1501 tttattttta ccacgtagta cagatgcaac aactgttgaa agacgtccca atggaacatc
     1561 agttgcgtct actacatacc atttacgttc tacttcgcct ggtttggcca tatatgttgt
     1621 acgcacgtta ttttcctcca atttaagttc tgatgtttga catacacttc gagtttccgg
     1681 ggctctttgt ggggcaaaca ataccattta acatattacc cgctaatcta gtcaatgtca
     1741 acgtttttct gcttgattga aaaatttata tgaaacttta cttcttttct ttcttttata
     1801 taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc
     1861 acaacccctt attttg
//
LOCUS       DACEPW010000315         1837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.323, whole genome shotgun sequence.
ACCESSION   DACEPW010000315 DACEPW010000000
VERSION     DACEPW010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.323"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW819_002783"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW819_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1245861.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="IW819_002784"
     CDS             complement(481..675)
                     /locus_tag="IW819_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245862.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            758..1366
                     /locus_tag="IW819_002785"
                     /pseudo
     CDS             758..1366
                     /locus_tag="IW819_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1386..>1837
                     /locus_tag="IW819_002786"
     CDS             1386..>1837
                     /locus_tag="IW819_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1245863.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGG"
BASE COUNT          603 a          360 c          362 g          512 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttaccca gaaaatcaac tttttgcagg
      781 agatgtatta gaaattgtat taaacgacca agcaggagaa gctgctgcta agggtgtgca
      841 gaacaagcca agtaccaacg atgaaattgg gaatcacaat ccaagtgata cgccagttgt
      901 ttatcatgat gccccgcctt ttgcacctgc tacgaagatt gtagtagaag gtggtctctc
      961 atttcttgcc ccttccaaac tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga
     1021 agcatttggc caaatggata tacaggaaaa attatggatt tatgatcagc gagaaacaaa
     1081 aagtccatgg atcttaacga taaaattatc tcagcctttc acaaccgatg caggattttc
     1141 tatgcaggat gtcctatatt tcaagaaagc tgaggagtat caattagtga atgaagaaga
     1201 actggaaatt gtcagaggac aaaattcaac gaatgtgcaa acagattatt cagaatattt
     1261 aaataatggt cgggcgttta aacttgtctt aaccaaagaa aatcagatag ctggtaatta
     1321 ctcagcagag atgacatgga cattagcaga tgcgccgaca gaataaagat caaggagaat
     1381 gattgatgaa tcgaatatgg aataaaagtc atcgaaacaa ctgctcttta cttctgatcc
     1441 ttctccttgg attcatcctt tggcagacac ctgtccatgc ctccgaattc aattttgcgg
     1501 tagattttga attaccggct aatcagattg ataagaacaa aacatatata aatgtaagga
     1561 tggagcctgg gaaaacacag gaaatcccct acacggtctt taaccgaaca gataaatcag
     1621 taacagttga gacattgatc cactccgcta cgacgaattc aaacggtgtg atcgaatatg
     1681 gagaatcaaa aagtaaattt gatgaaacat taagttatga tatgaaaaac ttagcaaaaa
     1741 cagtgggaga aataactgtc cctcctcaaa gtgcggtcac tgagacactt tcactgaaag
     1801 ctccagaaaa agagctggct ggtgtggtcg caggagg
//
LOCUS       DACEPW010000316         1826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.326, whole genome shotgun sequence.
ACCESSION   DACEPW010000316 DACEPW010000000
VERSION     DACEPW010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.326"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            173..655
                     /locus_tag="IW819_002787"
     CDS             173..655
                     /locus_tag="IW819_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245864.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            852..1742
                     /gene="pstS"
                     /locus_tag="IW819_002788"
     CDS             852..1742
                     /gene="pstS"
                     /locus_tag="IW819_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1245865.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          608 a          308 c          369 g          541 t
ORIGIN      
        1 tagttttatt gtaaattgaa tcctgcaata atcaattact ttttcattat ttctagatta
       61 aatgaataaa ctctcatgcc attatctcta aaaaaaaagt ttttccccct aattttttta
      121 gatagaaaat tgtatagtat gatgagagaa aaagtagttt gggggaacgt taatgagaag
      181 aaaagcagta atcgatcggc tttatttatt tttttggttt tttcctatcg ttttgctacc
      241 ttttacatgg gtaaatgctg ttgtacaagg tgcggagata ttaggcctga tccaaggaag
      301 cggatttgtg gtttctagga attttccagc tttgactgtt ttctatttag tgataagtgt
      361 aggtctctta ttaccaaaat ctctgatcaa tcactacaat tggcagctgc tttacttttc
      421 ttgcttatta gtatttacat ctatgttttc tttgctgcct cgcttgatta tcggccctaa
      481 tggtttgagc gagagtggat tgaaggattc agcttattcc tccatgtatt tcttgacgat
      541 caaaccatgg ttctatttag taattgcctc atttattctt gcaacatttt tttacttctg
      601 gacagaaatc aggataaaaa gtgaacgatc tgtatctata aaaaataact attgatggtg
      661 gatcatcagt aaaaaagcat atgagctgcg taaaatgcat gtttctcata tgcttttttt
      721 attattatgt aaagaatgcg aaaaacgttt acatgatttt tacataactt aactatattt
      781 ttacagcaac catacgaggg atttacagtc gactgataaa ctttagttgt aaataagaaa
      841 ggagcaataa aatgaaaaaa gtattgttta ctctaggtat gatgggaatt cttttagccg
      901 gatgcggctc aggatcagga aatacagatg gctctgccac acaaaataat tcaagtgact
      961 caaatgaaca agtaaaaatc gttgcggtag gttcgacagc attacagccg ttagtcgatg
     1021 cagcacaaga aagctttgta caagaaaatc caaattatca aatctctgta caaggaggag
     1081 gaagcggaac tggtttgagc caagtcgaag ctggagcagt tactatcgga aactctgatg
     1141 tatttgcaga agaaagagac ggagttgatg cttcaaaatt agtggatcat aaagtagcag
     1201 tggtcgggat ggcgccaatc gtcaataaag atacagatgt caaagatatc accaaacagg
     1261 aattgattga tatctttaca ggaaaaatca ctaactggaa ggaagttggc gggaaagatc
     1321 aaaaaatcaa cgtcgtgaac cgtgcaaacg gcagcggtac ccgcgcgaca ttcgaaaaat
     1381 ggggactgga tggtgctacc cctgtgcagt cccaagaaca agattcatca ggaacagttc
     1441 ggcagctggt aagtcaaaca ccaggagcaa tcagctattt agctttctca tatctcgatg
     1501 attccacaca agcactaagt atcgacggtg tagaaccaaa agaagagaat gtagcagata
     1561 acagctgggg aatctggtca tatgaacata tgtacacgaa cggaaaacct tcccctgaag
     1621 tccaaaaatt cttagactac atgatgacag aagaaatcca agaaggacct gtcaaagaat
     1681 tgggctattt accaatcaca atgatggaag tagaacgtga tcacgaagga aatatcaaat
     1741 aactagcaga taaacatgcc acggtagtcg ttactattaa ggaaaaatca actatgtttc
     1801 ccacatatac atttttcctt taattg
//
LOCUS       DACEPW010000317         1817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.327, whole genome shotgun sequence.
ACCESSION   DACEPW010000317 DACEPW010000000
VERSION     DACEPW010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.327"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            274..453
                     /locus_tag="IW819_002789"
     CDS             274..453
                     /locus_tag="IW819_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245866.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            512..868
                     /locus_tag="IW819_002790"
     CDS             512..868
                     /locus_tag="IW819_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245867.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          630 a          234 c          350 g          603 t
ORIGIN      
        1 ggtggtgcga aataaacggg caaagcccga taacttaaga gaaagaaggt gatccatttg
       61 aacaagatta ttcccttatt gttagtactg tttgttggcg aaatacttat gcgagatagt
      121 gcagctgtac agttaaacct cgctgtattt ttggtaatac tggtgttttg tttgatcttt
      181 tggcggtttg tattagtcct cgtgagtcta ctattcaaga tgattgcagc catatttttc
      241 atagggttat ttcgatgaaa ggagtgagta ccaatgccaa ttgcaatagg aaataaacgg
      301 ttacccgtga cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtat
      361 ggcaaaagtg aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa
      421 gcaggatttg atgtaccagc gctaaaaaag tgacgtcact ttttaatttg atctatataa
      481 gtgatatcac ttttaccaag gagggaatta aatgttaaaa actaaaaaga attcgtacaa
      541 attaaagctt agtatactat ctattatttg tcttttaggc atgataatta gtggtcattt
      601 gtatagagtg gcatatacgc agtttattga gtacaaggtt cactctttag gattagctgg
      661 ggatcgatac gttgaatatg tccattattc attaaaatgt tcttcatata ttcttctttg
      721 gcttattttt tcattgtttg aattgctaaa tttaatagca atatcttcat atcacttaga
      781 taagctaaat accaaatcta gccgttataa cgaagtgttt aaggaaaatg tgaagttaga
      841 aagtgactta aacaagctaa aaaaataaaa agaagaaaac ataagttaac taaaattatt
      901 ttattaagaa tgatggaata taaggataag gttaccaaca taacgatcat tacttctctg
      961 atatgctatg gacttctatc aatcagtatt ttgtctatag attatggagt aactatactg
     1021 caagccttag ttttggctat gcctatgtat ctatctttgt ttatcatgca tgaaatatat
     1081 aagctatttg ttttatcaaa aaatgtagaa atacgggtaa atattttggt gaatagttat
     1141 caaaaagaac tcaaagaaag aagacggtgg cggtatttat tgaaaggatg aaaaagtagg
     1201 atttgacata ctggtattca gaaagtgccg tcacttttaa aacaatggaa aaggtggaga
     1261 taagatgaag aaaaaacaaa ctaaactatc attagcagat atttaacaca gttaaccgca
     1321 actgaagatg gtgttgttga atttgaacaa tctgaaattg cggtagtaga tgatcgttac
     1381 cttaaaatgc cttacttctt tgaccaagca aaggtcattt gcctttgtca ctacgatggg
     1441 gtacgtgatt ttaggcgtta aaaagtagtg attatatcaa ctttacgagt aaatataaat
     1501 ttagacatga aactgaatta tccaatggaa aatcatggtt tataatctag aaaaattata
     1561 gtaatgattg gattatatat attctttcgt attttcgtac aactgtagct tgtcaaaatt
     1621 gagaaattta accgattagg tactttacat cgttatttgg gtaatttcta aaacatgaag
     1681 cttaccaacg aataaactga gactatctta ttaattgatc aatataagaa aatatctttg
     1741 aggagatgat gtactatgtt ttcgtctatc cacgattggt taggtggtct tttcggcggt
     1801 ggaggtaaag gttttta
//
LOCUS       DACEPW010000318         1806 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.328, whole genome shotgun sequence.
ACCESSION   DACEPW010000318 DACEPW010000000
VERSION     DACEPW010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.328"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..1508)
                     /locus_tag="IW819_002791"
     CDS             complement(27..1508)
                     /locus_tag="IW819_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1245868.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          565 a          235 c          311 g          695 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
     1441 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
     1501 attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag
     1561 tggaaagtca tttcagtctg tgttattctc acagagtgtc gccttctttt ttatataacg
     1621 ttatttaatt tttttgttac cttgtttatt ctattgttta tttcctaggg tattttgtca
     1681 agatattctt gtttttaagg cggaaaaatt gatttttaat ggatataact aaatatgttt
     1741 tgataattat caataatatt tttagaatta tttatgttat ttatttatgg gtagatgaaa
     1801 agagac
//
LOCUS       DACEPW010000319         1788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.329, whole genome shotgun sequence.
ACCESSION   DACEPW010000319 DACEPW010000000
VERSION     DACEPW010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.329"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            156..371
                     /locus_tag="IW819_002792"
     CDS             156..371
                     /locus_tag="IW819_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245869.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(588..844)
                     /locus_tag="IW819_002793"
                     /pseudo
     CDS             complement(588..844)
                     /locus_tag="IW819_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1031..>1788)
                     /locus_tag="IW819_002794"
     CDS             complement(1031..>1788)
                     /locus_tag="IW819_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1245870.1"
                     /translation="IDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIE
                     KRMSNLEQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHIT
                     EGKKTQSISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTE
                     EITDFLETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVST
                     KIKYLDNVSKTTSYFQYKLVLQKNGNWKIVDVK"
BASE COUNT          556 a          296 c          287 g          649 t
ORIGIN      
        1 aggcttaata ctatgaaaat tttctttagc atttttgatt taggttcctt ttctaattat
       61 aataagatga ccactacagt aacctactga aatatataaa cagccactgt agtaccattt
      121 tcaaagtagt ctatgagagg agaatcttca ttcttatgaa cgctggaatt gcggatatga
      181 atttaataaa aaaaaccctt aatgatttta cttccaattc aatatcaaaa ggtactggta
      241 tcaatttaag cacaataaaa aagctgaaaa gtggtgaaag atcagtggaa aagctgaatc
      301 ttttagatgc tataaaaata acggaatttg ctatgaaaaa tggaaaagct gagattgaaa
      361 tatggagata attatcgtct agtgaattac ttgccactta gctgaattta acgatccttg
      421 gtgcttgaag ggggagattc ttgggaatag agcatacttg caagcctatg tctttactaa
      481 caacgatatc tcttattaac caagctatcc ccgtagttct aaggctttta cttttatcta
      541 agcaataatc gtattcaaag gaaatttttg acccttagaa agtctcattt tatcagacgt
      601 gagcttaggc attgctgaat tttgttaatt cggttttagg ttgtgctttg agcataatat
      661 gaaagtattc tttatcacga tttcattcat ctaaggtaat gtgatgtgat tctgaaattc
      721 tttcaaaggc agtttttgca taagctgaca ttacatcatc aatgacatgt ttacaatgtt
      781 tcactactaa aataaggggg taaccaagaa gaaaaactga atgattattt gtatctaatt
      841 ccatggtata tcaaccattt cctatataga ctgattatga catggattaa aataaaaagg
      901 cactatgtct taattgtcgg ttttcgagtt accgaatggc attcatcacc cacctataga
      961 ggatgggcga cttctgcctg aaataagtta cactacttat aacttctaat aaaattagat
     1021 tgagtcatta ttatttaaca tcaacgattt tccagtttcc atttttttgt aatactaatt
     1081 tgtactgaaa gtagctagtg gtttttgata cattatcaag gtattttatc tttgtactca
     1141 ctagaaaatt ttttccgttt tgtagataaa ctggattctc taactccgaa aactttaagt
     1201 caatattgat tggtcgcatc gcaccatttt caacataata ctccaattct ttttcagaag
     1261 ctgatggata aagtttaaaa aatgtttcta aaaaatcggt gatttcttcc gttgttttgg
     1321 catcaatttc tgaatcgttt tctacttgtt ttgcttcata ttttgccttg tctggctttc
     1381 caatcattgt tggcaaacta gttactaaat gattcctgtt ttcatcttca aagattgtca
     1441 ctcgatatgc agaagaaatt gattgcgtct tttttccctc tgtaatatgt tgctccacta
     1501 aaaatgaaac gacaaattct tccgagtgtt tttcaacatc tagaattttt acagattgaa
     1561 cttcggaact cgtgggaata tctgctcgaa ccatatcttg gattaaagcg agaccgtttt
     1621 ctgtaaggta ctgttcgaga ttactcattc tcttttcaat tgcttctttg ttatttttcc
     1681 atgaaaaata ttctttcgca aaatcctctg taaaactttc aacgccagaa gtattcacta
     1741 ccttctcttt tataaccact ttttcatgag ttgtatgttg atcaatgg
//
LOCUS       DACEPW010000320         1781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.330, whole genome shotgun sequence.
ACCESSION   DACEPW010000320 DACEPW010000000
VERSION     DACEPW010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.330"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..513
                     /locus_tag="IW819_002795"
     CDS             <1..513
                     /locus_tag="IW819_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1245871.1"
                     /translation="VLQGIYFFNDTFTKEEKQKNFDFYEPMTVHESSLSPSIHSILAA
                     ELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIVQGFAQMKTAHDQLR
                     FAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEIYVYEQSYSLADRLE
                     ITLRKEEANV"
     gene            506..1171
                     /gene="pgmB"
                     /locus_tag="IW819_002796"
     CDS             506..1171
                     /gene="pgmB"
                     /locus_tag="IW819_002796"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1245872.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDRTFNEQLK
                     GVSREDSLSLLLAHGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            1164..>1781
                     /locus_tag="IW819_002797"
     CDS             1164..>1781
                     /locus_tag="IW819_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1245873.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPT"
BASE COUNT          563 a          374 c          367 g          477 t
ORIGIN      
        1 gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa acaaaaaaac
       61 tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg
      121 attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct
      181 cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat cacttccatg
      241 actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag
      301 ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat
      361 cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa
      421 ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa
      481 ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt
      541 cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg
      601 cattacgatc gatcgtacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact
      661 ctctttgctt ttagctcatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga
      721 attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga
      781 tgtcttcccc ggagtcgtgc ctttactaga ttctttaaaa gaagaaaaaa tcaagattgc
      841 tcttgcttct gcaagtaaaa acgggccttt cctacttgaa aaaatgggtc ttacacctta
      901 ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt
      961 cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc
     1021 gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc
     1081 tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta
     1141 cttgaaagaa aagtggcatg accatggata atccagaaat ctccgtcgta ccagttccag
     1201 agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt
     1261 acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt
     1321 tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg
     1381 tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa
     1441 actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag
     1501 tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt
     1561 ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag
     1621 agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct
     1681 acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt
     1741 tagatggtgt gcttgaatta gatcaagaaa aactgcctac t
//
LOCUS       DACEPW010000321         1779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.331, whole genome shotgun sequence.
ACCESSION   DACEPW010000321 DACEPW010000000
VERSION     DACEPW010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.331"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..343)
                     /locus_tag="IW819_002798"
     CDS             complement(<1..343)
                     /locus_tag="IW819_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1245874.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIG"
     gene            complement(530..1180)
                     /locus_tag="IW819_002799"
     CDS             complement(530..1180)
                     /locus_tag="IW819_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245875.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
BASE COUNT          605 a          360 c          224 g          590 t
ORIGIN      
        1 ttccaataga aaaatcacta taaatactct ctagcatagg accgacaatt gaagaagtca
       61 ctactgcaaa tgctggacct atccaagcaa cattagaatg ttctttctct cttttccttt
      121 gtaaatcttt gttgagtatt aatctattta tttcttctgc aggcaagtta attttcataa
      181 ccttatgggg gattatccaa ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt
      241 gatctgtgtc aatcagataa taacctgatt ttgattccag aataacatat ctcaaagccg
      301 aacgaaaatt tttcatgaat atccataatc tcactgtttc cacacctaat tcctccaatt
      361 ccaattaaat ggatttatag catcaccaat tatgttaact gcctgaccaa cctcattgat
      421 aaaattggca ccactatccc atgcttcaac aatgctatat atctatcaaa caaaaaatgg
      481 aaaactaaat actcgcgcac ataaatttta cattctgttt tttataagac taataataat
      541 cattgaaaaa atttcttatc ggtggcgcat attcccaatt gattaatgtg atcccatatt
      601 ctgcaagtat tttacgagct tcttctaagt catagtgaga aaaatgcata aaacctgcaa
      661 tacgatcacg gactgtgagc tgtggcttgc ttccaccatc attcataaaa tatccagcag
      721 agccactact taaagtgatt gttacatctt cactttctat tattaagaat tcataatgaa
      781 atgctttcaa ctctccacta ttatttaaaa gttttatact ccaacttcgt ctaggttgag
      841 ttgtcacatt ccctctaaaa cttcctataa attctgcaat caccgtatct tcaatcaaaa
      901 ttctttcctc atctatttca tattcaagac taaacggaaa ttctccatat gtaatctctg
      961 gcaatggtgg tgctgtagtt ctccaaataa atacaataac tgtgaataga aaaaatccaa
     1021 cgatcgatat aaatgttatg agacctgctc gaagtaaaac ccgatataat aaaaatgctg
     1081 tatttcgctt tgctatatct agaaaaataa aaagtactgc aatcataatg atagtaaaag
     1141 ttgtaaaaaa gcacattttt tgccctccct ttataaacat cgattctcaa attctaaacc
     1201 aaatcttgat agttcaagtt caggtataac gtataccaac catacatccc aattactatt
     1261 tatgaactta tgattatcac tgaataaatc actccatccc cttgttaaaa cttctacatt
     1321 aattacacct ggttgttttc gactagctaa tccaatgaac ttatcaacca tccttatcca
     1381 ccaaccacga gaatcttgac cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt
     1441 tccttccagt ttttaattaa tctccagtta tagataaata cttgatccta tctatatcag
     1501 gacttctata ttaagcaaaa gtttatgtat aaaactaaaa tcttcattca gttgtctccc
     1561 cgctttttat acgattgtat taaaaaacaa tagaagcctt tcaacattat gttactccag
     1621 taaagttgga attatagttt gacggaagaa aagcaagtat agcttttaaa aaaaactgat
     1681 acattcgcat ctcctgcttc atgtatcagt tttttctttg gattaaactg cttcttcacg
     1741 cagtttttcc gcaaattcat ttattttgcg aattcggtg
//
LOCUS       DACEPW010000322         1769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.332, whole genome shotgun sequence.
ACCESSION   DACEPW010000322 DACEPW010000000
VERSION     DACEPW010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.332"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            70..1101
                     /locus_tag="IW819_002800"
     CDS             70..1101
                     /locus_tag="IW819_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1245876.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            1098..>1769
                     /locus_tag="IW819_002801"
     CDS             1098..>1769
                     /locus_tag="IW819_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1245877.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAI"
BASE COUNT          515 a          288 c          349 g          617 t
ORIGIN      
        1 tattcgggaa agggtttgtg acaaaacatg tcacaaaccc ttttcatcat ttcatagaaa
       61 ggataaagaa tgcaaaaaaa aattagcatc atcactatat tttttatttg tgtgcttact
      121 tcatgggtta ttcagcatca agccgcttta tatgaagtac ctgtcatcga gatagaaaaa
      181 gtacgaataa aagaggatca tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa
      241 ggagaatcga tttatttatc ggttccttat caaaaaaatc aaatagaaac agcaaatata
      301 aaagcgagac aaatgtatct agtccaaatg catggagatg aatggcagct agtagcaaaa
      361 aaaaatgatg gctggctttt cttcttcaca agtatcttca ttggcgccat gttgcttatt
      421 ggcggaaaac agggtctatt tgctttagga agtctcatca tcaatatcgt tttactttta
      481 cttttgttgc tcatctttac taaaacagaa gggatttctc tattaaataa atctatcctg
      541 ttcgtcgtta tagggatgat tattagcata ttggctttag atggatggaa tagaagcagt
      601 ataatcaaaa tttcagctgc agttacgagt gtttttttag cattttttat ttgtcttttg
      661 acaatgaatc attggaaaga ccaaggactg cgctatgagg aactgaatta tctgacacga
      721 ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa cagctggagg aacactcgat
      781 accgttatca cagtcattgc gacattagaa gaattgacta agcgacaacc agaaataaca
      841 gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc
      901 aatatccttt tgttcgtcta tatcagtggg tcagttccat cgcttctttt atatttagga
      961 aatcaatggt ctttcaaaga aacgatagaa cagcatttat cattggaatt tttacgcgtc
     1021 attgctggca gcttaggaat agtattatcg atccccatct cggtcctctt ttttctgact
     1081 gtaaggaggc tgaaacgatg aatgtactta ttatacttgt tgggatttta gcaatcagcg
     1141 tgctacttat tggcggtacg caaggtatgt atattctttt aggcttattc atcaatttag
     1201 ggatcttttt cctgttgtta ttcggttatc atcagaagtg gccgatactg gcattgtcgg
     1261 tcataggatt tttattgatt gctgtggtta tcttattttt tatcaacggt tataatctaa
     1321 aaatgcgtgc agcttttgtg agtatactga tctttttatt ttgtttccta ctattgcttc
     1381 ctatcactga ttttttagcg atccaaggtt ttacatcggt agaactagaa gaattatctg
     1441 gcttagataa gactttagcg attgatttta gattacttac gagatcgttg cttctgatta
     1501 gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat
     1561 atcaggcaaa tccgcaatta tctttttacc aattgcgcca ttctagtttc gctattgcta
     1621 aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc attcatggga agttctttag
     1681 cgttgatcct ctggtttatt gatttagaca ttccttttca gcaagtgatc aatgaaaaat
     1741 catttgttct agaatatacg atggctatt
//
LOCUS       DACEPW010000323         1761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.333, whole genome shotgun sequence.
ACCESSION   DACEPW010000323 DACEPW010000000
VERSION     DACEPW010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.333"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            270..866
                     /locus_tag="IW819_002802"
     CDS             270..866
                     /locus_tag="IW819_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1245878.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            879..>1761
                     /locus_tag="IW819_002803"
     CDS             879..>1761
                     /locus_tag="IW819_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1245879.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMN"
BASE COUNT          666 a          276 c          357 g          462 t
ORIGIN      
        1 agaacacgcc gcactgcaaa ataatggagg gttaatctta tgcgtaatac cacacacaaa
       61 caacaagaaa tcgaaaatca aaaagccaaa attcgttcag aacaggaaaa aatgcgtgcc
      121 agcaaacgaa gactgcagca attgaagaac cagcagagga ttcaaaaaga aatcgaatta
      181 gatcgataaa atcttcctaa ctattgtata cgtataaact tatgttatac tgtttttata
      241 agtttatacg tatacaaagg agggagaaaa tgacaaaagc aatggtatac aagctgctag
      301 aagaatttaa tggaactttg gctttgaagg atgccaaaaa agcgggcatt tctcctgtaa
      361 cgatcaaacg tatggtagat cgaggggagc tggatcgaga gtatccaggt tttttcacct
      421 tacctggtca atttcctgat gaattgttta tggcgcaaaa aaaatatgaa cgcggcatta
      481 tttcacatat taccgcgtta gatttgtatg atttaacaga tatgatccct agacagatcg
      541 atctgactgt accttacggt tatcacgttt ctgaaaaagg attgaaggaa ttcgcagttg
      601 agctacacta tacaaaagcg gaatggtatg aattaggtaa gattgaaata aaaagtcgct
      661 atgggaatcc tattatagct tatgatccag aaaaaacctt gtgtgacatt tggaatccct
      721 ggtataacgt tgaggatgaa atcaaagtaa aagcaatcaa aaactacatg gagtcggaca
      781 gaaaaaatct tagaaaattg aatgagtatc gacgaatttt gcctacagat aaaacgatgc
      841 gctcttatat tatggcttta aattaaagga gaatacacat gacacctacg caattaaaag
      901 caaaagtgaa gaatatttct agagaaaaag aagttgatcc gcagcttgtt ttaagacatt
      961 ttatgatgga aaaattcttg gaaaaaatct cagagtctcc ttatcgtgaa aattttgtgt
     1021 taaaaggcgg cttcttaatt ggctcaaagt atggcattga aaatcgaaca actaaagaca
     1081 ttgatacgac tttacgggaa atgaaagtaa ccaaagaaac attgacaacg gttctaaacg
     1141 atattttttc aacgccgacg aaagaaggca ttcaatttga gattcaagga atgaaagaaa
     1201 cgagggaagc tgattattat ccagggttta gcttacgtgt actggcacat ttggaaaaca
     1261 tgcggccaga ttttaaggta gatgttacta caggtgactc gatctatcct gcgacgatca
     1321 ctcacagtca taaattgatg tttgaggatc gaacgattcc tttggaatcc tatcctacag
     1381 aacaaattat tgctgaaaaa ttaagtgcaa cctttgattt tttaacagat aattcgagag
     1441 gaaaagattt ttatgatctc tatactattc ctaaaatgga gaagatcgat gagaagacct
     1501 tgaaagatag tgtgagaaat acatttacac gtcgaggaaa aactgatccg ttaaagatat
     1561 actatgaaaa ggatatggat gtactgaaaa atagtcacga gctaaaagat acctggaaga
     1621 aatatcaaaa aagcaatcct tatgctgcgt cgatcacgta cgatgagaca atggattcga
     1681 ttagtaattt gatggaaaaa gtgattcacg tcgaagaaaa agagcgaaaa ctagcttatc
     1741 aaagacacca acagatgaat a
//
LOCUS       DACEPW010000324         1747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.334, whole genome shotgun sequence.
ACCESSION   DACEPW010000324 DACEPW010000000
VERSION     DACEPW010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.334"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1285
                     /locus_tag="IW819_002804"
     CDS             <1..1285
                     /locus_tag="IW819_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1245880.1"
                     /translation="ISLSIKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGNVK
                     GEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGV
                     NDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDAD
                     NEKGADETVEVEIKGLFDGHNSGVVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQD
                     ATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGS
                     LIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFL
                     AQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFM
                     IYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            1415..>1747
                     /locus_tag="IW819_002805"
     CDS             1415..>1747
                     /locus_tag="IW819_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1245881.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          670 a          227 c          321 g          529 t
ORIGIN      
        1 cattagttta tctattaaag atgctacgga cagagcttca aaagaaacat ttgctaatat
       61 aacaaatagt ttttctatgg agataaatag acaagtaaat ccaggaacac ctagaggtgg
      121 gggaaatgta aaaggtgaag atattaaaaa gatatctcaa acagatagta tagactccta
      181 tgtaaaaaga ataaacagtg ttgcagattt agttgattat gatattattg aaactcaaga
      241 gactcttgcg aatcaatcac ctgaaagagc aaagaatttt aaaagaacag ttatgttaac
      301 tggggttaac gactcaacaa aagaaacaaa atttgtatca gaagcatata aattagtaga
      361 aggaaagcat ttagaaaatg aagataaaaa taaaatctta atgcataaag atttagctga
      421 aaaaaataac cttaaagttg gagataagat aaaaataaaa tctaatttat ttgatgctga
      481 taatgagaaa ggtgcagatg aaacagtaga agtagaaatt aaaggtctat tcgatgggca
      541 taatagcggt gtagtaagtg cagcacaaga actatacgaa aatacattaa tcactgatat
      601 tcatacagct gctaaagttt atggtaatac cgaagataca gctgtatacc aagatgcaac
      661 attctttgta aaaggtgata agaatcttga tagtgtaata aaagatcttg ggaaattaga
      721 tataaattgg agagaatata atttgattaa aagttcatca aattaccctg cattacaaca
      781 atctatatca ggtatttact caatttcaaa taaattattt tttggctcat taatatttgc
      841 aggagttgta gtttcattat tattattctt atggatgaat gctagaaaga aagaaatagc
      901 agtattacta tcattaggta tatcaaaatt agaaattttt ggtcaattcc taattgagat
      961 ggtatttata tcaatccccg cattcgttgg ttcctatttc ttagctcaat atacagctga
     1021 caagcttggg aataatatat tgaataaggt tactggtgat atagctaaac aaatagctaa
     1081 acaatctgca tctagccaat taggtggtgg ggcagaagct gaaggattta acaagacatt
     1141 atcaagttta gatatcaatg tattacctaa attcatgatt tatgtagtta tatttatgag
     1201 tttagtactt cttgtatcat tgattatttc ttcattcaat atattaagaa ggaatccaaa
     1261 agaattatta attgacaata attaaaattt tcggtgcttt ttagcaccaa aaatgagagt
     1321 gtaatataaa ccgtgtaagt aaccatcaat cctaggcggg tggttgcttc acggttttta
     1381 tttttctaaa ataagatcaa gaggtgatta cacgatggct agaaagaaaa gaaatcctga
     1441 tgctgagaag ttagctgaat ccattttgaa tacttatcaa cccgaatctg tagaggatat
     1501 gcaagatgtt ttaaaagatg tgtttggtcc cttatttgaa aaaatgcttc aaggagaatt
     1561 gaataaccat ttagggtatg acgcccattc taaagagcca aagaaacatg acaatcgacg
     1621 aaacggttat ggcaataaga cgcttaaaac gagctttggt aaagtaaaca ttgatgttcc
     1681 cagagaccgg gaggcttctt ttgaacctga gttgattcct aatagaaaac gagatgtctc
     1741 cgacatc
//
LOCUS       DACEPW010000325         1745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.335, whole genome shotgun sequence.
ACCESSION   DACEPW010000325 DACEPW010000000
VERSION     DACEPW010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.335"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..540
                     /locus_tag="IW819_002806"
     CDS             <1..540
                     /locus_tag="IW819_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245882.1"
                     /translation="PKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDE
                     TDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLIS
                     PISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEK
                     ILLPENWAELRKLVEYGII"
     gene            527..>1745
                     /locus_tag="IW819_002807"
     CDS             527..>1745
                     /locus_tag="IW819_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1245883.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNI"
BASE COUNT          568 a          228 c          278 g          671 t
ORIGIN      
        1 cccaaaatag atgaaaacag taaattgatg aaaaacatac aatcaatctt tgggatagaa
       61 gaagtttttg tcggagataa atgtatcttt tttgatcaac ctttattatc tgatggttat
      121 ggcatagatg aaacagatat tttcgctaaa gtaaatagta tattttctgg aaaaaaagaa
      181 actttaatta agcttcatcc tagagcaagg acaaatcgtt atggtgaaaa aaacatattg
      241 aattccagtt taccgtttga agtcttgcta cttgaaaatg acttctcttc aacacttttg
      301 attagtccta tatctacaat ttctttttca ccatctatga tgtttgaaaa gacgatcaac
      361 tctattatgt tggctaaatt aattttaaat gaatatacag cattatctga agataaaaaa
      421 gaaacattga caaatattgt tcttttctgt gaacattata accgtctatc agaagaaaaa
      481 attttactcc cagaaaactg ggcagaatta agaaagttgg tagagtatgg gattatttaa
      541 cacactttcc aagtcaaaat tagttaagtc cagtttctgg tatactatag ggaatttttt
      601 tattaaggga atttcattca taactattcc tatttttgta aatattacta ctcaagcaga
      661 atatggtttg attaataatt tcacttcatt tgtatcaatt ttttcgattc ttgttgggct
      721 aagcctaaat tcggcaatta ataatgcaag ttttgactat aaagatgaca taaatgaatt
      781 tatgtcatcg gtactttctc tagctacact ttcgttaatt gtgttcttat taatagggaa
      841 catatatttt ttgttttcaa atgctttttt tgatatgaac caacttgtat ttaatttgat
      901 gttattacaa agttttgcta gttttgtaat tagttatatc cagtcttatt ttacgattaa
      961 ctttaaccat tttaaatatt tgattttatc ttttatatct acttttttga atattgcaat
     1021 ctctgtagct ttgatgcaga cattatattt tgatgataaa tatgttggca gagtagtcgg
     1081 cggaacactg tcatttgtgt tgataggttt agtaatttat ttaagaataa tgataaaggg
     1141 tgctcatttt tttgataaaa aacagtggag atacgcttta aaaatctctt tgccgctaat
     1201 cccacattcc ttagcaaata tagttttagc acaattcgat agaattatga ttaattctta
     1261 tttaggtcct gctgcggggg gaatatattc ctacattagt aacataggta ttattctaag
     1321 cgtaatatgg atgtctacta ataacgcctg ggttccatgg ttctatggtg agatgagtaa
     1381 aaataatgaa aaaaaagtga agaaagttag taactattat ttagtagtct ttactttgat
     1441 tacgatgatt gtaatggtag tttcgattga tttagcaaaa tttatggctc cgaaagacta
     1501 ttattctggg ttatctttag tggttccgat aaccttaggc tatttctttc agtttttata
     1561 tagtttacca gttaatgctg aattttatga gaaaaaaaca tcgtttattg cgattggtac
     1621 agtagcaagt gctatagtta atatactatt aaacatgatt ttcataccta ggttaggaat
     1681 aattgctgct ggatacacaa ccgtatttac gtatttttta ctttttattt ttcattataa
     1741 tatcg
//
LOCUS       DACEPW010000326         1731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.336, whole genome shotgun sequence.
ACCESSION   DACEPW010000326 DACEPW010000000
VERSION     DACEPW010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.336"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..454
                     /locus_tag="IW819_002808"
     CDS             <1..454
                     /locus_tag="IW819_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1245884.1"
                     /translation="RQNISQSLYYLKEALSYSSQKERIHVSDTEIQLISCIGKIYGVA
                     GKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEIDEALEQVNQGIY
                     WAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISKNL"
     gene            530..1678
                     /gene="nagA"
                     /locus_tag="IW819_002809"
     CDS             530..1678
                     /gene="nagA"
                     /locus_tag="IW819_002809"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1245885.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
BASE COUNT          596 a          284 c          377 g          474 t
ORIGIN      
        1 gcgtcaaaat atctctcagt ccctttatta tttgaaagaa gcattaagct actctagcca
       61 aaaagaaaga atccatgttt ctgacacaga aatacaactg attagttgta tcggaaaaat
      121 atatggtgtg gcaggaaaaa cggctgaagc acgctactat ttgagtcgaa gcatccaact
      181 gctgcatcaa tcagctgatg aacgatcaaa aagtgaattt tcgcaaattt tttataatta
      241 tggaaatttc ttattccatc aaaatgaaat cgacgaagcg ttagaacagg tgaatcaagg
      301 aatctattgg gctcaagaga agaatagcta ctattacctc aatgatttat ttgtgcttaa
      361 aagcttaata tacaagcgta aagaagaacc agaaagagca gcattctatg aaggcttagc
      421 acaagctgtg aaacaaattt caaaaaattt gtaaatggtg tagaccattc ttgacttctt
      481 ctctaaataa gagtacttta gaggtagaaa aacaaaggga gagaatagaa tgagaacttt
      541 tatctatgct gacaaattct ttttagcgtc aggtgtcaaa catgcaggct atcttgaaat
      601 taccgatgga ctttttggtg aatacaccaa agaactacct gagggagaag tcaacatcat
      661 tgaccaaagc ggcaaatgga ttgctcctgg tttggtagat acacatattc acggctatat
      721 gaatcatgat gtaatggata atgatgcaga aggcatcaaa gtgatgtctg aaggtctgtt
      781 atcttgtgga gtgacttcat ttttaccaac aacattgacg tcaagcaaag aacgtttaaa
      841 agatgtagct gagacaatcg gcaaaatgta tcaggaagca ccaggagcta aaatcaaagg
      901 aatctatttt gaaggtcctt tcttcacaga agaacacaaa ggggcacaaa acccaagtta
      961 tttcggggat cctgatttgg atactttcca tgaatggcaa gaagcttcag gtggtttaat
     1021 caaaaaaatt gctttagcac cggaacgtca aggtgtagaa gagtttgtca aacaagtgac
     1081 tgaagaagga gtagttgttt ctctaggaca cagtaatgct acactagaag aagcgcaaaa
     1141 agctgttgaa gcaggcgcaa gtgtatttgt ccatgctttc aatggtatgc gtggattgaa
     1201 tcatcgcgaa ccaggtatgg tgggtgcgtt attatcttta gaacatgtct tttctgaatt
     1261 gatttgtgat ggccatcatg tacatccaaa tgctgcggga atcctaatgg aaaaagcagg
     1321 acatgatcat gtcgcattaa ttacagactg tatgatggcc ggtgggatgc ctgatggaaa
     1381 ttacaattta ggcgaatttc cagtagtagt aaaagaagga acagcacgtc tagaatctgg
     1441 caacttagct ggaagtatat tgaaattgaa agaagcaatt aaaaatgttg tagactgggg
     1501 attagcaaca ccagaacaag cagtcatgat ggctagtctg attccagcaa tcagctgtaa
     1561 aatcgatgat acatgtggaa aaatcgctca aggacgcgat gcagacttta tcgtattgaa
     1621 ccctgatatg acattggaag caacttatct agatggcgtg gaacgtttcc acgcataata
     1681 taatttaagt taaccttaaa actaaacttc ttaagtttgg ttttaaggtt t
//
LOCUS       DACEPW010000327         1679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.337, whole genome shotgun sequence.
ACCESSION   DACEPW010000327 DACEPW010000000
VERSION     DACEPW010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.337"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..108
                     /locus_tag="IW819_002810"
     CDS             <1..108
                     /locus_tag="IW819_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="ATP-binding protein; PstABCS is an ATP dependent
                     phosphate uptake system which is responsible for inorganic
                     phosphate uptake during phosphate starvation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245886.1"
                     /translation="AFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            124..882
                     /locus_tag="IW819_002811"
     CDS             124..882
                     /locus_tag="IW819_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1245887.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            895..1572
                     /gene="phoU"
                     /locus_tag="IW819_002812"
     CDS             895..1572
                     /gene="phoU"
                     /locus_tag="IW819_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1245888.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
BASE COUNT          591 a          292 c          358 g          438 t
ORIGIN      
        1 gcctttttct atctaggaaa agtaatggaa tatgacaaaa ctaacaagat atttaccaga
       61 ccaaaaattc aggcaaccga agattatgtg tccggtcatt ttggttaaga cggaggcctc
      121 aacatgacaa aagaaattat ttcatctaaa gatcttcatc tgtattatgg aaagaaagaa
      181 gcactaaaag ggatcgatct aagtttcaat caaggggaaa tcactgcgat gatcggtcct
      241 tctggttgcg gaaaatccac ttatctgcgt tctttgaacc ggatgaatga cctgattccg
      301 aatgtaacga tcaccggaag tgttttatat aaagacaaag acatctatgg tccaaaaaca
      361 gatgttgttg aactaagaaa agaaatcggc atggtattcc agcagccaaa cccatttcct
      421 ttttctatct atgaaaatgt catctatgga ttgaaattaa aaggggaaaa agataaaaaa
      481 gtccttgatc aagtagttga agaaagcctg aaggcagcgt ctgtttggga tgatgtgaaa
      541 gacaaattgc ataaaagtgc tttatcttta tccggcgggc agcagcagcg tgtatgtatc
      601 gcacgtgttt tggcagttaa tccggaaatc atcttattgg atgaaccaac aagtgcgctt
      661 gatcctgttt ctactgggaa aatcgaaagt atgctgttag aattaagaga gcggtatacg
      721 atgatcatcg tgacccataa tatgtctcaa gcttcacgta tttcagataa gacggcattc
      781 tttttagatg gtcacttgat cgaatacaac gatacgaaga agatattcat gaatccagaa
      841 aaacaagaga cagaagatta tatttcaggt cgatttggat aaggaggaaa cggaatgcta
      901 cgtacacaat ttgaagaaga attgctgaat ctccataatc agttctacga aatggggatg
      961 atggtgagca gcgccgtgca caagtcagtt cgtgcataca tcaaacacga taaaatctta
     1021 gcccaagaag ttatcgaaaa cgatataaat atcaataaca tggaaacacg cttggagaag
     1081 aaaagttttg aaatgatcgc attgcaacag ccagttacaa ctgatttgcg tatgatcatt
     1141 acggtcatga aagctagctc ggatttagaa cgtatggccg atcatgcggt gtctgttgct
     1201 aaatccacga ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa agagatctcc
     1261 gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc tagttgctta tgtcaaaaca
     1321 gacgaaaaag acgcacgtac gatcgccaat atggatcaaa gagtcaatga ctatttcaat
     1381 cgaatctata atgcaacaat caaaaatatg caggaaaatc ctgagacagt catcagtgga
     1441 acagactact tgaatgttgc cagttatcta gaacggatcg gagactatgt gacaaacatc
     1501 tgtgaatgga tcgtctactt ggctacaggg aaaatcactg agttaaatac gaaccacaat
     1561 gagattttct aaactgaagc tgtgattatg ccgtttgttt atgaaaagag gttgtgaaaa
     1621 agctgttcta catcaagtaa taagaacagt caaacaaaaa atagctcctc atatttttc
//
LOCUS       DACEPW010000328         1675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.338, whole genome shotgun sequence.
ACCESSION   DACEPW010000328 DACEPW010000000
VERSION     DACEPW010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.338"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(228..1520)
                     /locus_tag="IW819_002813"
     CDS             complement(228..1520)
                     /locus_tag="IW819_002813"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1245889.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          471 a          360 c          294 g          550 t
ORIGIN      
        1 ttagtgccgt agatgcaagc ttttgaacac cgtttgttcg gaaaaaggga ctgtgacacg
       61 gtttttgtca cagtccctta ttctgaataa acagtgttca agagacagcc tatcggcaat
      121 aaggctaaac gtctctttca ctacctcttc aagttattat tatcgtatag ataaacactt
      181 cgactcgctc tggtaagaac gtcctgattc agtcgtcgtt tttatcctta gatttgcgcc
      241 cagacgcttt ctaagatgtt tgtttgtgtt ctatcaggtc cgacagaaaa tgttgagata
      301 cgtacaccaa caagctctga gatacgacgt acatagttac gtgcgttttc tggcagttct
      361 gcaagtgttt tgcatcctgt aatgtcttct gaccagcctg gcaattcttc atagattggt
      421 ttgcagcggt tcaactcttt caagcttgct ggatagtggt agattagttc gccatctaat
      481 tcataagccg tacaaatttt tactgtttcc aatccactta atacatcgat cgagtttaaa
      541 gataggttgg taatacctga aactcgttta gaatggcgca taactacaga atcaaaccag
      601 cctacacgac gcggacgacc agttgtcgta ccgtattctc gtcctacttc gcggatttgt
      661 tctcctgttt catcaaataa ttcagttgga aatggtccat caccgacacg tgacgtatag
      721 gctttgcaga cgccgacaac tttattgatt tttgaaggcc ctacaccgct tccgattgtt
      781 accccaccgg ctactgggtt agaagaagta acaaaaggat aagttccctg gtcgatatcc
      841 agcattacac cttgtgcgcc ttcaaacaac acgcgtttgc ctgcatccaa agcatcattc
      901 aagataacgg aagtatctgt gacgtattgt ttgatttgtt gtccgtattc ataatattct
      961 tcaaaaatat cgtcaaaatc aagtggttct gaatcaaaca tttttacaaa ttgacggttt
     1021 ttgtcatcta gattcaaacg aagacgttct tcaaaaattt ctttatctaa taagtctgca
     1081 atacggatac ctacacgagc tgctttatcc atataggcag gtccgattcc tttaatcgtt
     1141 gtaccaatct tattttcgcc cttagcatct tcttgtaatt ggtccaattt gatatgataa
     1201 ggcaagatta cgtgcgcgcg gtcagaaata cgcaaattac ttgtttctac acctttttca
     1261 tgtaaataag ctaattcttt aaccaatgat ttaggattga ctacaacgcc gtttccgatc
     1321 acactgatct tgtctttata aaaaatccca gaaggaatca aatgcagttt ataagtcaca
     1381 ccgttaaact taattgtgtg ccctgcattg tcgccacctt ggtaccgtgc aatcacttca
     1441 gcattttcac tgagaaagtc tgtgattttt cctttccctt catcgcccca ttgcgtacct
     1501 actactacta cagatgacat gtgaacacct cgttctatat tatttaatcc tgaaccattg
     1561 tatcaataat agatacatat ttcaaataaa atcgaatgat ttttattttt attttataaa
     1621 aacgcttaaa aaacgaactt tatcttttta gaatggttat taaaaaagag gttgt
//
LOCUS       DACEPW010000329         1662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.339, whole genome shotgun sequence.
ACCESSION   DACEPW010000329 DACEPW010000000
VERSION     DACEPW010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.339"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            97..567
                     /locus_tag="IW819_002814"
     CDS             97..567
                     /locus_tag="IW819_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1245890.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            652..1260
                     /locus_tag="IW819_002815"
     CDS             652..1260
                     /locus_tag="IW819_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1245891.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1394..1594
                     /locus_tag="IW819_002816"
     CDS             1394..1594
                     /locus_tag="IW819_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1245892.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT          551 a          239 c          338 g          534 t
ORIGIN      
        1 aaaggatcaa aaaaaattaa tttttttaaa taaagatgaa agttattgaa atatagagta
       61 tgataaataa gaatttttaa taaggtaggt tggctcatga agcagtatcg acagttaaaa
      121 ggatatttag aagcaaaagg atattgggag atactagtct atctcttttt tggtggtttg
      181 gctacgattg taaattttgt tagttttgct ctcgcacgcc aatattttga tttaagtatg
      241 gccttatcca attctatttc gtggttttgt tctgtattgt ttgcttttgt aacgaacaaa
      301 ctttgggtat tccattcaaa atcaccgaat tttacacatg cacttattga atgcggtaag
      361 ttcttcttct accgtatcct ttcttacgga ttggacatgg gggcaatggt attgttgatc
      421 aatgttatga acagcaatga gtatgtggcg aaaatcatca ctcaaattat tgttatcctt
      481 gcgaactata ttttcagcaa gttgtttatt tttaaagaaa ctgaggtttt tgaagaagaa
      541 gtaggtcaaa gtgaaaaaga cgactaggca taacaattga agaatttcct tctctttgtt
      601 actctttgta atgtagacat tattaagaaa acattgagga ggaatttttt tatgacttac
      661 acattaccag atctacctta tgcatatgac gcattggaac cttatattga cgaagaaacg
      721 atgcatctgc atcatgataa gcatcacaat acttatgtga cgaatttaaa ttcagcaatt
      781 gagaaatacc cagaattagg cgaaaaaaca atagaagaat tattatctga tatggacgct
      841 attccaacag atatcaagac agctgtacgt aacaatggtg gcggacatgc taaccattca
      901 tttttctggg aaatcatggc accaaatgct ggtggcgaac ctacaggaga aataaaagaa
      961 gcgattaatg aagcttttgg tgatttttct tcttttaaag aagaattcaa aaaagcagcc
     1021 gctggacgat ttggttctgg atgggcttgg cttgtaatgg aaaatggaaa attagctatt
     1081 acctctactg caaatcaaga ttctccattg atggaaggaa agacaccaat tctaggtttg
     1141 gacgtttggg aacatgctta ttatttgaaa tataaaaatg tccgaccaga ttatattgca
     1201 gcattctgga atgttattaa ttgggatgaa gtaaacaaac gtttcgaagc agcaaaataa
     1261 ccatttcctc cgtcatatca gtcttaaaaa taatttggaa aataaaagag cgaaagcttg
     1321 ctagcgtttt ctaaattggg tataatgttc ttgcaaggtt acttgcaaga acttttttgg
     1381 aggatataca tttatggaac acggaacggt taaatggttc aacaatgaaa aaggatttgg
     1441 gtttattaca gtagacggcg gagatgacgt atttgttcat ttctcagcaa ttcaaggaga
     1501 cggcttcaag agcctagaag aaggtcaaga agtagaattc acgattgttg aaggtgcacg
     1561 cggacctcaa gctgctgaag tgactaaatt ataataattt gttgaataaa gacacgactt
     1621 cttagagagt cgtgtctttt tgtctattta tgaagttttt ag
//
LOCUS       DACEPW010000330         1658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.340, whole genome shotgun sequence.
ACCESSION   DACEPW010000330 DACEPW010000000
VERSION     DACEPW010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.340"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            484..1569
                     /gene="xerS"
                     /locus_tag="IW819_002817"
     CDS             484..1569
                     /gene="xerS"
                     /locus_tag="IW819_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1245893.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          621 a          282 c          226 g          529 t
ORIGIN      
        1 tcaatctcag cagttaagat ttttttgaat tgttgcttga attttttctt tatctaaaaa
       61 aattgtatct ccaggatgca acccattttc tttaagaaat tccttttaaa ttgttaatac
      121 tactgagtgc cccacttctc tgaattttct ttaacttctc cctccccttt tttatttgta
      181 tatacattca tatacgcttg ttaatatcaa atcaaccgta gtctctctta tgtctaatta
      241 tatagaaaga ttgttctttc tatataattt agacaattta aaaaattaag attgcctcct
      301 tctgtttcct ttcctattca tgtataccat ttattttaaa ttattaaatc tatacctatt
      361 tatctgcttt tttgaaataa attaataaat ttcagagtat accaattgac aaaacactct
      421 ataattggta tagtccaatt atagagatac tcctctcccc tacttatcga aaggatgaat
      481 aacatgaaaa atacagaaat atatcaaaaa atagatacaa tcatacttca tatgcccgat
      541 tatataaaag attttgttca agatcgtgag gataaagatc aatcacctcg aactacttta
      601 gaatatctaa aaaattataa gctcttttat gaatggcttc tgagtgagtc tatcgttccc
      661 gatacttcaa tcacctcgat aagacagtta actgcctatg atctaaactt gtacaaatct
      721 catttgaaac gtcgcacaaa ggaaaatgta aaaaaagaga caacaaaaac taagctcaaa
      781 gataataata acttaggtct atctacttct actatcaata gaaatatcac agcattaaag
      841 gttctattta aatatctttc gaaaagttcc aataatcctt taggaaaacc atacttagaa
      901 aataatccta tggatcaagt agcaactatc acagataaaa ctacgttagc tgcccgtgca
      961 aacgcaatcg agaaaaaatt atttctagac gaagatacac aaaattatct agattatatt
     1021 gccaatgaat ataaaaaaac tttgagcaaa cgagctttga tctattatca acgagatgta
     1081 gaaagagacc tggcgatcaa tgcattgatt ttaggatctg gattaagact ctccgaggtt
     1141 gtaaatatta atttagagga cctatcttta gaaaagaata atgtggtagt aactagaaaa
     1201 ggaaataaga gagacgcagt aaatatagca gcatttgcaa tggaatattt agctaattat
     1261 ctagctattc ggaaagaacg atacaaagta acagaaaacg aaaaagcttt atttctagca
     1321 atttatcaag gagaagccaa gcgtatatcc ggtattgcaa tcgaacggat ggtagcaaaa
     1381 tattcaaaag gattcagagt ccaagtcagt ccacataaat tacgtcatac tttggctact
     1441 agactctatc aacagacaaa ttcattggta ttaactgccc aacaattggg acatacaagt
     1501 acaaatacaa caactttata tactcatata gacaatgcag ctacaattga tgctttgaac
     1561 gctttgtaaa tgtagtaaaa aatagtataa taatgaagcc tgtctctaaa attagataaa
     1621 agcccaactt taaaggcaag cttcattatc atttttat
//
LOCUS       DACEPW010000331         1641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.342, whole genome shotgun sequence.
ACCESSION   DACEPW010000331 DACEPW010000000
VERSION     DACEPW010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.342"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..862
                     /locus_tag="IW819_002818"
                     /note="aph(3')-IIIa"
     CDS             68..862
                     /locus_tag="IW819_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1245894.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(970..1158)
                     /locus_tag="IW819_002819"
                     /pseudo
     CDS             complement(970..1158)
                     /locus_tag="IW819_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1138..>1641
                     /locus_tag="IW819_002820"
     CDS             1138..>1641
                     /locus_tag="IW819_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1245895.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPES"
BASE COUNT          538 a          271 c          394 g          438 t
ORIGIN      
        1 tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa
       61 ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg
      121 ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa
      181 tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga
      241 acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca
      301 ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg
      361 ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg
      421 catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag
      481 ccgattagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa
      541 ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga
      601 aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt
      661 gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg
      721 gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt
      781 cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat
      841 tttactggat gaattgtttt agtacctaga tttagatgtc taaaaagctt taactacaag
      901 ctttttagac atctaatctt ttctgaagta catccgcaac tgtccatact ctgatgtttt
      961 atatcttttc taaaagttcg ctagatagag ttctatatta gagatgtaaa gagttttggt
     1021 gaagaggttt gtgagtatgg cttttataac cagggtgcaa tgagaagcac taacagcagc
     1081 tagcttaaga acgcatagga tatacttttt atggaagtca cagaggagga ataaaatttg
     1141 aacaggatta acagagtaac ctctattctt attcagctgc aatcaaaaaa aataattcct
     1201 gccaaagaaa ttgcacagcg atttaatata agcttaagga cagtttacag agatatccgg
     1261 acacttgaag aagccggaat accaattgga tctgaggccg gaaaaggata ttttcttgta
     1321 gagggcttcc tacttccgcc ggtaatgttt acagcggcgg aagtgggtgc attgattaca
     1381 gcagggaaat ttttaaattg ccatggagat gaatcattta taaaggattt tgattcagct
     1441 atgtataaaa tcaaatctat tttgaagcat ggtgaaaaaa actatgcaca ggagttggag
     1501 aacagtatta acgtgtacag cacatctgga cagaaaaata cattggcgga taacgttatt
     1561 gcagcaattc agactgcaat ctgcaataaa agggtaatat caattcaata tcctgcatca
     1621 ggagggcaag aaccggaaag c
//
LOCUS       DACEPW010000332         1636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.343, whole genome shotgun sequence.
ACCESSION   DACEPW010000332 DACEPW010000000
VERSION     DACEPW010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.343"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            146..1630
                     /gene="guaB"
                     /locus_tag="IW819_002821"
     CDS             146..1630
                     /gene="guaB"
                     /locus_tag="IW819_002821"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1245896.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          563 a          285 c          378 g          410 t
ORIGIN      
        1 acaatctctt ttaatacaca cagatgtaaa cgctttatat tggtattcta tgagaaagct
       61 aacttgattc caagtagatc attgacttaa atgaaaaaat caattatttt aatagataaa
      121 ctaaattaga ggagtggtac taaaaatgtc caactgggaa acaaagttca cgaaaaaagg
      181 atttactttt gatgatgtct tattgatacc agcagaaagt cacgtgttac caaacgaagt
      241 ggatatgagc gtacaattag ctaaaaatat taagttgaat attccaatca tcagtgcaag
      301 tatggatact gttaccgaca gcaagatggc cattgctatg gcaagacaag gcggactagg
      361 tgtgatccat aaaaacatga cgatcagcca gcaagcggat gaagtaagaa aagtcaaacg
      421 ttctgaaagt ggcgtaatca ttgatccgtt cttcttaacg ccacagcatc ttgtagcaga
      481 cgcagaagaa ttgatgagca aatatcgtat cagtggtgtg ccaatcgttg aaacacttga
      541 aaacagaaaa ttggtaggga taattacaaa ccgcgacatg cgttttgtaa cagattatca
      601 catgccgatt gctgatgtga tgacaaaaga caacttagtg actgccccag taggtacctc
      661 tctaaaagat gcagaaaaga ttttgcaaaa acataaaatc gaaaaattac caatcgtaga
      721 taacgaaggt cgtttaagcg gattgatcac gatcaaagac atcgagaaag tgatcgaatt
      781 tccaaatgca gcaaaagatg aacatggtcg attgcttgta gcagcagccg ttggtgtaac
      841 aagcgataca tttgagcgtg cgaatgcttt attagaagca ggggcagatg caatcatcat
      901 cgatacagct catggacata gtgcaggtgt gatccgaaaa attcaagaaa tccgttcaac
      961 ttttgcagat gcaacattga ttgcaggaaa cgttgcgacg gctgaagcga caaaagcttt
     1021 atatgatgca ggtgtagatg tcgtaaaagt tggtatcgga ccaggatcga tttgtacgac
     1081 acgtgtggta gccggtgtag gtgtacctca gctcacagct atttatgatg cagcatcagt
     1141 tgctcgtcaa tatggcaaag cgattattgc agacggaggg atcaaatatt ctggtgacat
     1201 cgtcaaagca ttagctgccg gcggacatgc agtgatgcta ggaagtatgt tggcagggac
     1261 agatgaatca ccaggtgaat tcgaaatctt ccaaggacgt cgattcaaaa cataccgagg
     1321 tatgggctca ttaggcgcga tggaaaaagg ttcaaaagac cgttacttcc aaggtagtgt
     1381 caatgaagca aataaattag taccagaagg aatcgaaggt cgggttgctt ataaaggaag
     1441 tgttgcagat attattttcc aaatgatcgg cggtttgcgt tcaggaatgg gttatgttgg
     1501 ggcagccaat ctacagcaat tacgtgatga agcgcaattt atccaaatga gcggcagtgg
     1561 attaaaagaa tcacaccctc atgatgtaca aatcacaaaa gaagcaccaa actattcagt
     1621 tgaatcttaa aaacat
//
LOCUS       DACEPW010000333         1579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.344, whole genome shotgun sequence.
ACCESSION   DACEPW010000333 DACEPW010000000
VERSION     DACEPW010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.344"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1086
                     /locus_tag="IW819_002822"
     CDS             <1..1086
                     /locus_tag="IW819_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1245897.1"
                     /translation="LLHVLNETVSEEDIYVSFETPNSEEIQQEVPTKLKNHPLVEPFE
                     LLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLMLIGTILAQKLLVLPRGM
                     KRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPILSTTDDVNTILILSVI
                     FGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVIALVGAMLIKNTAFVYL
                     GAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIGDLVSYTRLMALGISGG
                     SIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAYVHGARLQYVEFFGKFY
                     TGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            1105..1575
                     /locus_tag="IW819_002823"
     CDS             1105..1575
                     /locus_tag="IW819_002823"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1245898.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
BASE COUNT          432 a          293 c          362 g          492 t
ORIGIN      
        1 ttgctgcatg tgttaaacga aaccgtatca gaagaagata tatacgtcag tttcgaaaca
       61 ccaaactcag aagagattca gcaggaagtc cctactaaat tgaaaaacca tccgcttgtc
      121 gaaccttttg aattgctgac tgaaatgtac agtctgccaa aatatgaaga agtagatcca
      181 acaccttgga tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc agatgttggt
      241 tatggtttgc tgatgctcat tgggacaata ctagctcaaa aactacttgt tctaccacgc
      301 gggatgaaac gtttcgtaaa attctttgag attctctcga taccatcgat catctgggga
      361 ttgatctaca gttcattttt cggacagtca ttaccaaaag aactgtttgg catccgcttg
      421 cctttcccaa tcctatctac gacggatgac gtcaatacga ttttgatttt atccgtgatt
      481 ttcggtctga tacagatttt ggttggatta tttgtggcag caaaggaaaa tatcaggaga
      541 aaagattact tgagtgctgt cagtgatgga tttgcctggc aagggatcct aattgggatc
      601 gttattgcgt tggttggtgc catgttgata aaaaatacag catttgttta ccttggcgct
      661 gggctatcga tcattgctgc cttatgtatc ttgatcgttc cgattatcca aacgccgtct
      721 aaagtcaaag gtgctgccaa aggtgcgtat aatctttacg gactgaccgg ctatatcggt
      781 gatttagtca gttatacacg tttgatggca ttgggtattt caggcggaag tattggagcg
      841 gcattcaata tgctagttgc tttcatgccg ccagctgctc gattcagtgt agggatcctt
      901 ttgatcatcg ctttacacgc actgaatatg tttttgacac tgctaagtgc ctatgtccat
      961 ggcgcacgat tgcagtatgt cgaatttttt gggaagtttt atacaggagg cggacgtgcc
     1021 tttgatccgc tgaaaacggc ggaaaaatac gtcaacatca atcacaagaa aaaaaacaat
     1081 aaataattgg aggattttta tataatgatg gattacttaa ttagtcaaaa tggtggaatg
     1141 gtatttgcgg tattagcgat ggcaacagcg acgatttttt cagggatcgg ttcagctaaa
     1201 ggtgttggaa tgacaggtga ggcagcagca gcattgacaa caagtcaacc cgaaaaattc
     1261 gggcaggctc tgattttgca attgcttcca ggcacgcaag gtttgtatgg attcgttatt
     1321 gcctttttga tttttatcaa cttaggaaat gacatgtcag ttgtacaagg actgaacttt
     1381 ctaggtgctt ctctgccgat tgcttttaca ggcttgtttt caggtattgc ccaaggaaaa
     1441 gtcgcagcgg caggtattca aattttagcg aaaaaacctg aacatgctac aaaaggaatc
     1501 atttttgctg cgatggttga aacatacgcg attttaggtt tcgttatttc cttcctatta
     1561 gtgttgaatg cctaagcat
//
LOCUS       DACEPW010000334         1555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.346, whole genome shotgun sequence.
ACCESSION   DACEPW010000334 DACEPW010000000
VERSION     DACEPW010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.346"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(209..>1555)
                     /locus_tag="IW819_002824"
     CDS             complement(209..>1555)
                     /locus_tag="IW819_002824"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1245899.1"
                     /translation="PIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHL
                     GDWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKR
                     LEEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKH
                     LLNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGN
                     EQSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQ
                     SAKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPY
                     VQYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVI
                     AKHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT          421 a          336 c          273 g          525 t
ORIGIN      
        1 gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
       61 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
      121 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
      181 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
      241 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
      301 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
      361 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
      421 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
      481 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
      541 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
      601 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
      661 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
      721 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
      781 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
      841 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
      901 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
      961 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     1021 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     1081 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     1141 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     1201 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     1261 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     1321 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     1381 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     1441 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     1501 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attgg
//
LOCUS       DACEPW010000335         1552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.347, whole genome shotgun sequence.
ACCESSION   DACEPW010000335 DACEPW010000000
VERSION     DACEPW010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.347"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(59..1189)
                     /locus_tag="IW819_002825"
     CDS             complement(59..1189)
                     /locus_tag="IW819_002825"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1245900.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
BASE COUNT          443 a          314 c          244 g          551 t
ORIGIN      
        1 ctccactcca tgaaaagccg actttttggg acgctttttt ttgccaagcc tcacattttt
       61 atagccaaac ttcttttgcg acttgctgca ataattggat tttcttccat tgttcttctt
      121 ctgttaaatg gtttccttct tctgttgaag caaacccaca ttgaggactg atacttagat
      181 tttccaatgg tacaaattgt tgtgcttctt ttattcgttc ttttattttc tccgtatctt
      241 ccaaatctgg aaacttagaa gtaactaatc ctaaaacaac tttactttct ttgttactcc
      301 aatgtcttaa aggagtaaaa tctcctgctc ggtcagaatc gtattccaaa aagaaaccat
      361 cataatttgt tgaaaacaac tcttctgcaa tgggttcata tccaccggaa aacagccaag
      421 aagaagcgtg attcccgcgg cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt
      481 ccagtgcgtt attgataatc tctgtacaaa tatgggccat ttctttcgga tcaattcctt
      541 tttgaagaag tgcttctctt ttagtttgat cagctagaaa cgcccaattt gtatcatcga
      601 attgcaggta acggcaacct ttttcataaa aagcatgaat cgctgctttg taagtagagg
      661 acaagtcctc ataaaaatca gatagatcag gataaatctc cttgattttt gaagaaccat
      721 cattagatag gatctcttgt cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa
      781 caaaagtatc atctgtttcc actacttctt tcagaaaagc aaaatctttt aaaaatgggt
      841 gatttggatt gaaagataat ttatctacta cccgaatatt ataagctggt gctgcttttc
      901 cgtggaatgt ttgattatat ccagtttctg gaacatagcc ttcgatacca tttagttctt
      961 ctaaaaaatc aagatgccaa aatccacgcc gaaattcacc atctgtaatt cctttgatgc
     1021 cgactgcttt ttggtcttcc actaattttt ttatttcctg gtcttcgatt gcacgaagtt
     1081 cttcttttga aagtaagcct tcttgaactt tttttcttgc ttctttgatc gattgcgggc
     1141 gtaagaaact tccgacatga tctactgtaa atggtctatt tttgctcatt tcactcactc
     1201 ttttctttgt tttttgtaaa aaaaaatcct tttactagca aatactagta aaaggacgat
     1261 tatactcatg gtaccacctt ttttcagaaa aaacattctt tctctcagcc tgacaataaa
     1321 atcagtggtc ttggtaacga aagactgttc gtttttgcct acctttcgac aaaaaatcct
     1381 ctaaggccat tttcgacagt caacttctag ctttttacac caaccaaagc ctctcttgaa
     1441 ccattttctg tctactttcc ttctcatcgg atactattag aataaaatta gaatagcatg
     1501 tttgaaagaa aagtcaatag ggaataagaa caaacaaaaa ctaatttgtt at
//
LOCUS       DACEPW010000336         1530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.348, whole genome shotgun sequence.
ACCESSION   DACEPW010000336 DACEPW010000000
VERSION     DACEPW010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.348"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(185..1168)
                     /locus_tag="IW819_002826"
     CDS             complement(185..1168)
                     /locus_tag="IW819_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ1245901.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT          470 a          279 c          231 g          550 t
ORIGIN      
        1 gcttatttaa gaagaagttg cttctgctcc caccgtttat tcgtttttaa agaatgagac
       61 aaaagtgttt cttacttttg tcccacgctc ttattcatgc tcagagcgcc aatagataca
      121 aaatcatagc caaagtttca cgaaccctag ctgtcaaaga atcgatattt tattttaata
      181 taggctattt taaaataaat ggtttaaggt attcctctac aaaagccact ctatcaatta
      241 tcatgggttt aaaataagaa gtgatggaga taaccagatt gttcttagcg tcaatataaa
      301 gaacatttcc actattacca attgctgaat atatttcgtt ctcagaatct aatacccacc
      361 ataaatagcc atatctcata ttttgtaagt cttctaaagc actggttcta ggtttgaaca
      421 tctcgtttat ccacatgggt ggtacgattt gttttccttt atacatccct ttatttaggc
      481 ataatacacc aatttttgcc agatcacttg ccgataaaca caaaccatat cctgccgtcc
      541 cgatatttag tggatcacaa aaccagacat tggttttagg agacttatcc attgtaaact
      601 gcttatgttc ttcagctgtt tcagcataat aattgacatg gtttggtatc cctataggat
      661 caaatagata cttattggca aaatcaacag ttgttagctt acttgtctga tacaacaagc
      721 cagttaatat gtgtaaacag acagtttgat agttgaattc gccggttata ccttttcttc
      781 ctcccaataa atctagtgat gtttttgtcc aatcttcact tgaacagact tttgtccatg
      841 gatcgccttt acatttgtag ggtgctttca ttgttagcaa gtgcttgatt gtaatagcgt
      901 aaatagtttt ctccccgcgt ttcactttat actctggaaa ataatctaat actttatcat
      961 cgatactttt gattaatcct tgctctaaag caatgccaat cagcaaagat acaatacttt
     1021 tcgttgctga catgatatga caagtatcgt cttttttata accattccat tcatctgtat
     1081 aaacttctac cccatcctta tatgcgacaa tttgacatat atttggttgt cgaaaaccaa
     1141 taaaatcatg caactcttgt ttattcataa gatattacct cactttcttc tataagcata
     1201 atgtcattaa aaatcgattt caattttttc gattcttcac gtaatacagg tattcttatg
     1261 gactagacag cgctttggga agatggattc tctgaaaaat ataaaggttc actttatgag
     1321 tggataaaaa aagcagcaaa tacttcgact tctttaattt accaacatta gtgaaaatcg
     1381 attcatctgg agatttgtaa tttagagtcc ttgtcttgat tagaggtgta taaagctttt
     1441 tattagacat ttttaaattc cattacaatt tgcttataaa ctaggttttt aaaggtgact
     1501 atggtaagat aatagagata ttattttgtg
//
LOCUS       DACEPW010000337         1528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.349, whole genome shotgun sequence.
ACCESSION   DACEPW010000337 DACEPW010000000
VERSION     DACEPW010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.349"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            15..1481
                     /locus_tag="IW819_002827"
     CDS             15..1481
                     /locus_tag="IW819_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1245902.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          325 g          497 t
ORIGIN      
        1 ttggggtata gattatggaa aaacagcaag acaagggctt cttcgggcag ccgaagggat
       61 tgtcaacttt gttcttcact gagatgtggg aacgatttag ttattacggt atgcgtgcat
      121 tacttatcta ttatatgtac gatacagttg ccaacggggg acttggatta ccacgagcaa
      181 cagcattagc tatcatgtcg atctacggat ctttgatcta catgtctagt atcgtcggcg
      241 gatgggtatc tgaccgagtg ttaggagcaa aacgcactgt attcttcggt ggagttttca
      301 ttatgcttgg ccacatcgtc ttggctttac catttggtgt aagtacattg tttgtttcga
      361 tggcattgat tattatcgga accgcctttt tgaaaccaaa tgtttctggc atggtcggac
      421 atctttattc aaagactgac ttgcgaagag atgcaggatt ttcgattttt tacatgggtg
      481 tcaacttagg ggcattatta gcaccactaa tcgttggtac aatcggtcaa gaatataact
      541 atcatttagg gttctcttta gctgctattg ggatgttctt tggattatta caatacatca
      601 tccaaggacg caagacatta gacggagtgg gtacaacacc gccgaatcct ttgacaactg
      661 aagaacgtaa aggattcatc cgtaacttga tcatcgcact tgttgttatc gctgcagtat
      721 tcggcggggc acaactaact ggtcatctga caattgactt ttttgtcaat acaatcagtg
      781 tattggggat tatcttaccg ttgtattact ttatcaaaat gctgacttca aaagatgtaa
      841 ctgctgaaga aaaaccaaga gtatgggctt atatcccatt attccttgca gcaatcattt
      901 tctggtcatt acaagaacaa ggttcatcta tcttagcttt atttgctagt gaacgtacac
      961 aatctacgct atttggtgta acgatccaag caagctggta tcaatcattg aatccaattt
     1021 tcatctttat tctgacacca gtattcgtaa cgatgtggac gaaacttggt gaacgtcaac
     1081 catctacagt agtgaaattc tctcttggac ttttatttgc aggattatcg tacttgctat
     1141 tgatgttgcc gggtatgctt tatggtacaa gtggaaaagt aagtccattg tggttgttgg
     1201 gaagtttctt catcgtggtt attgctgaat tgtgtctttc accagttggt ttgtctgtga
     1261 caacaaaatt agcaccacga gcatttgaat cacaaacaat cgctatttgg ttcttagctg
     1321 atgcttcttc acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg ggaacagaat
     1381 ctgcttactt cggtattatc ggtgcagtag caatcgtcgg tggtatcttg ctatttactg
     1441 tgaaagaacc aatcaaaaaa ctaatgggct ctattcatta atcaatgaac ataaaaacag
     1501 gaattgctta tttgcagcag ttcctgtt
//
LOCUS       DACEPW010000338         1516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.350, whole genome shotgun sequence.
ACCESSION   DACEPW010000338 DACEPW010000000
VERSION     DACEPW010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.350"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(475..840)
                     /locus_tag="IW819_002828"
     CDS             complement(475..840)
                     /locus_tag="IW819_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245903.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(880..1182)
                     /locus_tag="IW819_002829"
     CDS             complement(880..1182)
                     /locus_tag="IW819_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245904.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(1182..1487)
                     /locus_tag="IW819_002830"
     CDS             complement(1182..1487)
                     /locus_tag="IW819_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245905.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
BASE COUNT          399 a          353 c          226 g          538 t
ORIGIN      
        1 taaacagata agaccgttga cgttatcgat ttttacattt gtgactgctt gactttctcc
       61 acgctcataa tattctacag ttgatactga tacatcagct tcggagttta attgcagaat
      121 ctgttactgc cgggataatt taatattctt ttcataattc accttttttc ctactgacat
      181 cgaatatttt tcatgttatg attagccgtg aaaggagagc tatattatgt gtttacacat
      241 gatgtcctta gctcattcgg ttctttggaa gcttttttgg aaataactct caggttcttg
      301 acgcaaatct catttattct ttgaaacaaa aaacttccat tttccgagag ctttggatca
      361 cttcaaaaga gatttgcatt tatatttctg tgaccttgag tcgcgtaaag aaccttacag
      421 caatggcggc aattccactt tcaggaaacc gccttttttc attgatattg gtggttagcg
      481 gactacccat tttctttcgg cagtgccaaa ccacaaatga tctgtgttga tgtttaggta
      541 gttgtatacc agataagcta aatccaaagt gcctgcatct tcaagacgac aatattccaa
      601 aaattctccc atagctggac ataaaaatcc tggagctatc atatcccaac agttaatagt
      661 gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt tcacgaatta caacatactc
      721 caattcttct ccgctggcag agcattccaa aacttcaaat tgattaagaa atggtagaac
      781 atatgcttca aacatcaaca ttccatcttt ctgtttttgc acttgttttg catcaatcat
      841 gtctatcctc ctattcagtt atttcttcca ttaatggaat tatttcaggt cactggactt
      901 aacgaaaaca ccattgacca tctttccagt ccgacctttg atttcgtcat aagcaaaatc
      961 caagcactcg tataaatcca tatcattttg cattgccaag atgatcaagg ttactactac
     1021 atcgccaatt ccgtctctta aaccatgttc atcttttcta gctagagaag cggcaacttc
     1081 cccgatttct tcgaccgttt ttaacatttg cttgctgaaa tccgcttgat ccaatccctt
     1141 atcttttgcc cactgctcta ctttcgcgat tagttcgtcc attatttctc ctccacatac
     1201 ctaaactgtc ttccttttga atcaatccat aagctcctag ctctatccca aataatattt
     1261 ttgctcagac cagtaatttc agataactgt ttagcggtac ccgttactag aattcgatca
     1321 ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg gcttttcagt ccacattgat
     1381 ttaccgagct tttggacttc tgcaactatt tctttgtctt cctgccaaga ttctgacttg
     1441 gttaattcag caattcgttt cattgtcgct ttcttatcca cgctcattcc tccaatctac
     1501 gaatttccct tcttaa
//
LOCUS       DACEPW010000339         1515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.351, whole genome shotgun sequence.
ACCESSION   DACEPW010000339 DACEPW010000000
VERSION     DACEPW010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.351"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..332)
                     /locus_tag="IW819_002831"
     CDS             complement(<1..332)
                     /locus_tag="IW819_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245906.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFS"
     gene            complement(632..1513)
                     /gene="galU"
                     /locus_tag="IW819_002832"
     CDS             complement(632..1513)
                     /gene="galU"
                     /locus_tag="IW819_002832"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1245907.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          421 a          341 c          231 g          522 t
ORIGIN      
        1 ctagaaaaag tagttgtatg tctacctttt ctgcctatcc gagactttct tttcatatat
       61 gggatataaa tgattagata cagtacagca aaaaataaag caaatacgca agcttcccaa
      121 ctacctaacg ccaattcaac catcaaggta gcaaccccaa cggatgctgc ataccagata
      181 aaaatcagaa acgtttcttt ttttctgacc atgcgaaaca tgataaatag actaataagc
      241 aatagtggca acacaaatat ccaattgctt ccttccatca gccacttgag tatatcaagc
      301 agcgactgtg caacagcgac taattttccc atagtgaaca cacacctttt tttataatta
      361 aaaaattcgc ctttttattc tactatgaat cttctggctt ggctttcatt tctcttatgt
      421 aatcattttc ttttatacaa cttaaaaaaa aattaaacca ttagcataat attttctgaa
      481 tctattcacg aaagtctttt tctatctttc gttttccacg actcaactga tctagagtca
      541 aaaaactaaa acgacagttg cgcaaatgac gcacaagctg ccgctttaat aacaaatagc
      601 ctttcattct cagcaggcca cttatttcca gttaactttc tgttcttagc tttttactca
      661 actggataat atatgcactt aatgaagtcc caatttccga atgttgaagt ccatattcaa
      721 tatttgtctt catatatcct aacttatcgc ctacgtcgta tctttttcct ttaaactctc
      781 tcgcatagac cgcctctgtc tggttaagag tctgaagtgc atccgttagc tgtatctcgt
      841 ttcctgctcc tggttcttga gtttctaata tctcaaatat tttaggagtc agtagataac
      901 gtccgataat ggctaaatta cttggtgctt ttcccatttc aggtttttct acaaaccctt
      961 ttactctgca acgacccttt tcgctatctg ttttaggatc aataattccg tatttagatg
     1021 tttcattttc tggtacacgc atgacggcaa cagcggaagc ttgtgtttct tcataatcca
     1081 tcatcaattg ttgagtcaat gggatatcat ctttcatcaa atcatccccc agcatgacga
     1141 caaacggttc atttcctata aatgcttttg cttgaagcac ggcatccccc agtcctcttg
     1201 gatgagattg acggatgaag tgaagattga cttgagtcgt ttcttcaact aaagcgagta
     1261 attctgtttt ttccttttcc cgcaaattgt tttccaattc gatatttgca tcaaagtgat
     1321 cttcaatggg tcttttttct tttcccgtta cgatcaagat gtcttcgatt cccgaatcca
     1381 atgcttcttc cacaataaac tggatcgttg gtttatccac gattggaagc atttcttttg
     1441 ccatggcttt tgttgccggc aaaaaacgtg ttcctagccc agcggcagga atgactgctt
     1501 ttttgacctt cactt
//
LOCUS       DACEPW010000340         1505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.352, whole genome shotgun sequence.
ACCESSION   DACEPW010000340 DACEPW010000000
VERSION     DACEPW010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.352"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            126..740
                     /locus_tag="IW819_002833"
     CDS             126..740
                     /locus_tag="IW819_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1245908.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            733..>1505
                     /locus_tag="IW819_002834"
     CDS             733..>1505
                     /locus_tag="IW819_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1245909.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQ"
BASE COUNT          514 a          227 c          285 g          479 t
ORIGIN      
        1 caaaacgaca aaatatgagg aactattttt ccgtttgttt ctctaataat caggtcaagg
       61 tgctgattgc ccaacctttt tttgtataat gagaaagtat aaacttaatg tttggaggag
      121 aagatatgtt agggaaggtt ttggaagagt tatttatccg gatctgggtc attataaaac
      181 taaccttgta tttctggata tatacatttg ccggcgggat catctttgga ttaggtgcgg
      241 catggaaaac agtcaatgaa ctattttatt tgtacggatt cgagtataaa gaaatcacaa
      301 taaaaagagg ctggaatata tataaaagaa attttttaag aggcaatctg ctatttagtt
      361 tatttttatc tggtactgct ttattgagtt acaatttata tttatctgtt caaatcaaag
      421 ggctcatttt tttagctatt gattttattt tactttttgc cttgctctgc ctatttgcta
      481 cttatcaata cagtatgatt ttagatagtg aatatacgat ctctgttcct aatctgctaa
      541 agctaagttt tatttcggtt ttttccagct tttcgtcttt tctaaaaata attattggta
      601 gcggtataat tttaggcgtg acttggcaat tcaaaggatt gatcctgttt ggtgtcattg
      661 gtcttttgac ggtttggaat ggtacgatga caacacattg gcgtgaagaa ttagataaac
      721 agttggaaag ttatgaataa gtttcggatt ctattttcaa aaggaacctt actaaatcgt
      781 ctgttgcgga aatacgcggt tatcatgatc ggtgtaacga tgactgctac cgttattttt
      841 agtatccata cttgggaaca aaatcaaaaa caggcagaaa acatgacaag tgatgccgtt
      901 caaagcacaa gcagaatgct gaatgataaa acaaccttga gtaggatcat caaaaaccag
      961 cttgtaggga attccgaaaa gatcgaaaat gtgacgacct acttaacaaa acccatagat
     1021 cagtatctga tgtatgtata cgaacagcaa aacagtaccg atgaattagt ttctttccca
     1081 aatcaaatca aagatctcta cgcaaattat gaagaactaa gtgcgatata tatcgtgttg
     1141 aatcaactac cggagtatta tgaatcttct agagaaaaca aaggtgggga aataaaaaag
     1201 gggacaccaa aattactaga cgccttttat atcaagttgc ctatcacaca aggaggagct
     1261 gagtcaggaa gtatttttat tggttttcaa aagaaagaac tagatatgat tttagaaaat
     1321 ctaacgtcat tcaatggact ctctttatac atgatttctg gaacgacaaa tcgactatac
     1381 acattccatg ataataaaat agctaaagaa acgtttgaaa gacaagaaat gatcatcaca
     1441 gagagtctga agaaaaattc acaattgccg attgacgatc tggaaaaaaa caattttgtt
     1501 caaca
//
LOCUS       DACEPW010000341         1489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.353, whole genome shotgun sequence.
ACCESSION   DACEPW010000341 DACEPW010000000
VERSION     DACEPW010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.353"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            242..967
                     /locus_tag="IW819_002835"
     CDS             242..967
                     /locus_tag="IW819_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1245910.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            1221..1487
                     /locus_tag="IW819_002836"
     CDS             1221..1487
                     /locus_tag="IW819_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1245911.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
BASE COUNT          514 a          235 c          318 g          422 t
ORIGIN      
        1 caagaggtcg tacagaagcc gtttagtttt gagtattaag aaaaattttt tgaatgataa
       61 taaatacctt gtaaatagtg ttttgtgatt gagtagagac gatgaatccg tctctttttt
      121 tgtattctta agccgtctaa aaactggttt tattttttta ctgtttctat ttctttccag
      181 tatttattta taggggaaca tatgttaaga tttagggaac tgttagtaaa ggagtaaaga
      241 aatgagcaaa aaaacaacca aggcgatcgt ggggatcgtc gctgctgtga ttgcaggggc
      301 actcggtatt tccaccacac aaaaagattc agatatcttc caacaaatta caaccatttt
      361 tgattccaca ccgcaagctt ctcaagtagc ggatgtgcca gaatatgatg gaaaacatca
      421 agaaatagaa atcaatggga atcagccgga gtttacaaaa gaggatctta gtctttccaa
      481 agggacatgg gaaagctatt caaatataga caaattgaac agagtaggac aagccaatgc
      541 gatgttggga aaagacttgt ttcctactga aaagagagaa cctttgtata ttgatccgac
      601 aggttggaaa caaaagaaat taagtgacgg acaatggtta tacaaccgca gccatttgat
      661 tggtttccag ttgactggtc aaaataataa cattaaaaac ttaatgacag ggacacgttc
      721 gttgaatgct ccgtacatgc ttgcccacga aaatgatatt gcggcatata tcaaggaaac
      781 aaatcatcat gtacgctatc gtgtcactcc tcatttcgag ggaaatgaac tggtagcaag
      841 aggtgtgcag ttggaagcag aaagcattga agacaaaaaa attgaattca atgtctttat
      901 ctacaatgtt caagatggct acaccatcaa ttatgagaca gggcaagcaa cgaaaacgaa
      961 aaattagtag cagcaattcg aattttttat gaactttatg aaaaaattgt gtaaaaacat
     1021 actttcaaag aaatatagcg tatactttat ggaaagaggg cttttgaaaa agccacagaa
     1081 aaaaggaaaa atcagacaaa tcgtgtttct taatttattc agaagtttgg aatatttgtt
     1141 tgtagttttc cctttcttcg agtaaaataa gggtgtagaa ggtaagctgg aattaccttg
     1201 cgggaaagag gtgtagaatc atgggtttta cagatgaaac cgtacgtttt ggttttgacg
     1261 atagtcgcaa aaaagaagtg agcgaaacat tagcgattgt ttatcgggct ttggaagaaa
     1321 aaggctacaa tccaatcaat cagattgtcg gctacttgct ttctggagac ccggcctaca
     1381 ttcctcgtta tcgtgatgca cgaaatttga ttcgccgtca cgaacgcgat gaaatcatgg
     1441 aagtaattgt caaagactac ctctcaaatc atggagtgaa tttatgaga
//
LOCUS       DACEPW010000342         1458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.354, whole genome shotgun sequence.
ACCESSION   DACEPW010000342 DACEPW010000000
VERSION     DACEPW010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.354"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            195..896
                     /locus_tag="IW819_002837"
     CDS             195..896
                     /locus_tag="IW819_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1245912.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          513 a          256 c          248 g          441 t
ORIGIN      
        1 ttattgttgt tttttataca caaatcattc gcttcaaaac aaattattca ataccattgt
       61 atatttttta cataataaca aattatattt gtaaagtatt aaaacttgtt tctaatgttg
      121 gtatgttaaa actttacaaa ttctttaaaa aaagtgacaa taaatatagt gaacaataag
      181 gagaggactt tttaatgcgt gttaataaaa tcgattttca cttttcaaaa ctcagatatc
      241 aacctgattt tgaacatttc tcggatgaag agttccaaca gatcaaggaa catactgttc
      301 ttcgttctta taaaaaagga cagatccttt tcgacgatgg tgatcccaga caacggttgt
      361 atatcttgac caaaggactc gttcgtttag aacgttatga tgaaagtgca acttattact
      421 actatgacta tgtgaataca aataaccttt ttcctatgtc aggaatgttt ggagaagaga
      481 cctacactta tacagctcaa gcgatgactg atattgaaac attctacctt cctgtttcct
      541 actatgaaaa attagcacga aaaaatcccg aacagttgat ctatatgata caaaaaattt
      601 ctaaagtagt tgaaatacat gaattgcgta tccaaatcgg attgacctct agcgcctttg
      661 accgggtaaa acaaaattta ttcattttga aagaagaatt gggcgtacca acaaaagatg
      721 gtcatatcac cattccttat ccgatcacct taaaagaatt agcagtgaat agcggcacga
      781 cacgtgaaac tgctggacaa gtaatcaaac aactacgtga ttccgggctg ttagattatg
      841 acaaaaagcg ttttactttc tgtaagacag ctactactcc agctatgaga gaataacgcg
      901 aacaaaaata agcagtgacc ctgataaata tcagggtcac tgcttatttt cctccctaaa
      961 acttctgaaa gatccggcag ttttagatca ctggacaaga ctcaaagatt ttctatgtgt
     1021 gtttcagtta tgtaatattc actgtttgca aaaaatactg ttagattaac tagatgaagc
     1081 gtcttgtcct catccgatct agattattcg atattcctcg acaataaacg attactcagt
     1141 gagggcaatc agaggaacgg ataatgataa ataagctgta aaaaatagca aaataacaat
     1201 aatgattaaa agtatgtaaa aaatagggct ttgacaaaaa cgtgtcacag ccctatttcg
     1261 attaaacggt gtgtacctgt caactcttcg gtcacaagtc ataatgttca taaacatgag
     1321 gagctgtttt ttgaaattgt tccttgtgaa tcagagttaa acgacaggct tacgaactct
     1381 tgattaaacg gcgttcaaaa gcttgcatac gcggtaataa gaacagccag gtcaaaaaca
     1441 tgagaagccg tttttcct
//
LOCUS       DACEPW010000343         1422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.355, whole genome shotgun sequence.
ACCESSION   DACEPW010000343 DACEPW010000000
VERSION     DACEPW010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.355"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1330
                     /gene="uxaC"
                     /locus_tag="IW819_002838"
     CDS             <1..1330
                     /gene="uxaC"
                     /locus_tag="IW819_002838"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1245913.1"
                     /translation="KNMPIIDYHCHLDPKEIYENNNFSNLTEAWLGGDHYKWRLMRAC
                     GVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWSHLELKRFFGIDLLINEENAEKI
                     WTQANEILAAPDFRRREIAKKSNVEVICTTDDPADDLHYHQLLAHEEPDLKVLPSFRP
                     DKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVLGQRIEYFHQHGCRVSDHALDIL
                     RYKEADETVLEEIFQKARANKLLSSDEIDAYLTETLIHLTHFYHSHNWTMQLHIHAYR
                     NCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAEETDQLPKTILYSLNPNDYPAIL
                     ALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQFADGSALGNFVGMLTDSRSFLSY
                     TRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQAISYTNAKNYFGFFA"
BASE COUNT          470 a          263 c          292 g          397 t
ORIGIN      
        1 aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa tttacgaaaa
       61 caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca aatggcgctt
      121 gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg attttgaaaa
      181 atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt acagttggtc
      241 acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag aaaatgcaga
      301 gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc gtcgggaaat
      361 cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag acgacttgca
      421 ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt cattccgtcc
      481 agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac tactggaaga
      541 agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag ggcagcggat
      601 agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca tcttacgcta
      661 taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag ctaacaaact
      721 attatcttca gatgaaatcg atgcttatct tacagaaaca ctgatacatt tgacccactt
      781 ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta actgtaatac
      841 acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg atctttcttt
      901 gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt tgccaaagac
      961 gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga tgggctgttt
     1021 ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca acgatactcg
     1081 agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg ggaattttgt
     1141 cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat atttccgtcg
     1201 tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg aagatgaaca
     1261 acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt actttggctt
     1321 ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt gaaataacgg
     1381 aatccgttta tccacgtttt agagaagaag acggaccaaa tt
//
LOCUS       DACEPW010000344         1422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.356, whole genome shotgun sequence.
ACCESSION   DACEPW010000344 DACEPW010000000
VERSION     DACEPW010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.356"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(150..1337)
                     /locus_tag="IW819_002839"
     CDS             complement(150..1337)
                     /locus_tag="IW819_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1245914.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          309 c          249 g          451 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tt
//
LOCUS       DACEPW010000345         1421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.357, whole genome shotgun sequence.
ACCESSION   DACEPW010000345 DACEPW010000000
VERSION     DACEPW010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.357"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..125
                     /locus_tag="IW819_002840"
     CDS             <1..125
                     /locus_tag="IW819_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1245915.1"
                     /translation="VALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            162..659
                     /locus_tag="IW819_002841"
     CDS             162..659
                     /locus_tag="IW819_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1245916.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            677..1387
                     /locus_tag="IW819_002842"
     CDS             677..1387
                     /locus_tag="IW819_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245917.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
BASE COUNT          529 a          254 c          291 g          347 t
ORIGIN      
        1 cagtcgcatt aaatggtctt tctgacgaag aagcaaaaca attggaatat cttttaaata
       61 aagtatgcaa gaatgttagc gaagattgga attttgtcaa aaaaggcaat aaaaggatct
      121 attgaaagga agaagccgtg aataaatacg aaattcgaaa aatgacgcct tcagatgtag
      181 acttactaca acaaattagt atcgaaacat ttacagatac atttggaaaa gataattcac
      241 cggagcattt agctgattac ttgacacaag cttatgcact agaaaaactt acagaagagc
      301 taaaagataa agaaaccgat tttttcctag tgagtatcga tcaagagcct gcgggttacc
      361 tgaaactaaa tacaggaacg agtcaatctg aggaaatggc tgatcatttt ttagaaatcg
      421 agcgaatcta tgtacgaaaa aaatttaaac atcaaggtat cggtacattt ttactagata
      481 cagctattga aaaagcaaaa gaaaagcaaa aaacagccat ttggctcggt gtttgggaac
      541 acaacgatgt tgcgctcgct ttttatcgta aaaaaggatt tatcaaatac agcgaacatg
      601 ttttttatat gggagatgat ccccaaacag actatctatt catcaagcaa ttattctaat
      661 caagaggtgt gaacagatga aaaaagtaat agtggatacc aatttctggg aagtatttcc
      721 tgaagcaacc atcgaaatct tatccgtaaa tggtatcgat aaccacgtaa caaaagaaag
      781 tgaagaaacg tatcatcaat tattgaatga ggcagcaaaa gaagcaagaa attaccttac
      841 agaagaaaca ttcagccaaa acgaagtcat tgctcagtgg cgtcaggcat ttaccacatt
      901 caagactaaa aaaggtgcac gttcttctat tgaagcttta ctgaaacgtg caaatcaaga
      961 acgggaattt ttcccaatca atccgcttgt agatatctat aatagtattt ccttgtcttt
     1021 tggcgtacct tgcggcggag aagatatcga aaagatcgca ggaaatctgc atttaggaaa
     1081 agcccaaggt ggcgaatcat tcctgccttt aggtgcagat tccgatgctc cggcacttcc
     1141 ggaagaaatc atctactatg atgaacaagg agcgatctgt cgctgtctca actggcgtga
     1201 agcccaaaga acaatgctga cggaagaaac acaagcagcg gtgctggtca ttgaatccat
     1261 caatgctgaa caagcggcac gtggcgcaca ggcaatcgaa gcgttgaagg aaaaaatcga
     1321 tcaaacattt ggcgtaacta gtgttagaca cacactaacc aaagatgaat cagaagcaga
     1381 attataaaag aatcgaaata agaagagcgg cattcattgc c
//
LOCUS       DACEPW010000346         1418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.358, whole genome shotgun sequence.
ACCESSION   DACEPW010000346 DACEPW010000000
VERSION     DACEPW010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.358"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..164
                     /locus_tag="IW819_002843"
     CDS             <1..164
                     /locus_tag="IW819_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1245918.1"
                     /translation="IKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLE
                     KRFSTAPLK"
     gene            273..1190
                     /gene="rbsK"
                     /locus_tag="IW819_002844"
     CDS             273..1190
                     /gene="rbsK"
                     /locus_tag="IW819_002844"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1245919.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            1218..>1418
                     /gene="rbsD"
                     /locus_tag="IW819_002845"
     CDS             1218..>1418
                     /gene="rbsD"
                     /locus_tag="IW819_002845"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1245920.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSI"
BASE COUNT          494 a          282 c          289 g          353 t
ORIGIN      
        1 atatcaagcc gaaactgaca acgatcgcac aaccgatctt cgaattggga caaacatctg
       61 ccaaattatt gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac
      121 ctgtacgatt agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta
      181 tatttttatc gaactattaa aacgttttac taaaacgttt taaaaaataa tttttcccat
      241 caaaataaat gagatagttg gaggtttgta aaatgaacac ggttacagta attggaagta
      301 ttaatatgga cactaccctt cgtttaacca acatgcccaa gccaggcgaa acgatgcatg
      361 cgcatgagat ttttcacgct ggcggaggta aaggcgccaa ccaagcagtg gcagccaaac
      421 gttcaggcgc acggacatct tttattggag gcgttggggc tgacagcgaa ggacaacagt
      481 tattagatct actgacaaaa gaaaacatcg atacatctgg gatagctgaa atccaaggcg
      541 ccacaaccgg acaagcaatg attatggtag acgcagcagg agaaaatagt atcttgatcc
      601 atgcaggggc aaataatgct ttccatgaac aagaagtcct aaaaaacaag caactgatta
      661 caaacagtga ctttattatt gctcagtttg aaagtagctt agatgcgacg atcctagctt
      721 tttctattgc aaaagacgca ggaaaaacaa cgatcctgaa tcctgcacca gctcgtgaaa
      781 cgattcctac tgaactactt gaaaagacag atatcattat accaaatgaa acagaaacag
      841 aaatcattac aggaatcaga gtgacagatc ataacagttt ggtcgcagca gccgaaaaac
      901 tccatgaatt aggtattgga acagtaatca ttacactagg cagtgccggc gctttctatc
      961 atacagaaaa agaacacggt atcgtccctg cttttaaagt ggatgccgtt gacacaacag
     1021 ccgcaggtga tacatttatt ggcgcattaa gcagcacact tcagccagac cttagtaact
     1081 tgaaagaagc gatcctttat ggaaatctag cctcttctgt tgctgtgcag tcttatggtg
     1141 cgcaaccttc tatcccttac cgcgaagctt tagaacaatc agcagtctaa aattaacaat
     1201 gagaggaaga aacaaacatg aaaaaaggaa aagtaatcaa ttcagatatt tcacgagtaa
     1261 tcgcacaaat ggggcacttt gataaattaa gtatcgggga tgcaggcatg cctgtcccct
     1321 ttggtactga aaaaatagat ttggctgtct caaacggtct tccaagcttt ttatctgttt
     1381 tagaaaacgt tttagaagaa cttgaggtac aatcgatt
//
LOCUS       DACEPW010000347         1418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.359, whole genome shotgun sequence.
ACCESSION   DACEPW010000347 DACEPW010000000
VERSION     DACEPW010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.359"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(387..1001)
                     /locus_tag="IW819_002846"
     CDS             complement(387..1001)
                     /locus_tag="IW819_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1245921.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          465 a          274 c          235 g          444 t
ORIGIN      
        1 atatcaggta aacttcattg caataatgat aacaaatgta ctataattac tttgtaaaga
       61 gttttgggga agactctttc atcaacgtgt attaagagac aagaaagaat ccatatggat
      121 tctttcttgt ctctttttcc actacttttt atttttctga cactacatat tcttataact
      181 catttctcat aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta
      241 aaatcaaaga tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct
      301 cattaataat cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt
      361 aaggaacttt gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt
      421 gctgtaaaat acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag
      481 atcgctggat ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc
      541 aaaaccaaat cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt
      601 cgtgacaaat caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga
      661 gctagttcga tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct
      721 gatttgacag aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca
      781 cctccggctg gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat
      841 tcaaatgcta caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta
      901 atttgtttcg cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc
      961 tcttccaatt ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct
     1021 atacttgaca tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt
     1081 tagacagtaa cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac
     1141 gcccaatacc ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag
     1201 gggtagaatt ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta
     1261 tggcaaaatg atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc
     1321 aactaagggt cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta
     1381 atctcttatc caagtgcaag caagaaaata caaaaaaa
//
LOCUS       DACEPW010000348         1416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.360, whole genome shotgun sequence.
ACCESSION   DACEPW010000348 DACEPW010000000
VERSION     DACEPW010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.360"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            88..1038
                     /gene="yidC"
                     /locus_tag="IW819_002847"
     CDS             88..1038
                     /gene="yidC"
                     /locus_tag="IW819_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1245922.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            1201..>1416
                     /locus_tag="IW819_002848"
     CDS             1201..>1416
                     /locus_tag="IW819_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1245923.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT          485 a          282 c          285 g          364 t
ORIGIN      
        1 atgaaatttc tatgaagttg attgaattcc atgcgctttt ttagtatagt taagtttgtg
       61 taaaaattat agaagaggaa gtattctatg aataaatgga aaaactggtt attgggatcc
      121 ggacttgtca gtatcctgct ctttttatct ggctgtgtgc ggatggattc aaacggaaac
      181 cctgacactt caggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca
      241 tatctagttg agaatttcaa ttggtcttac ggctgggcag ttattgcaat gactgtgatc
      301 gttcgtatca tcatcttgcc actaggtatc agtcaatcga aaaaaacaat gatccaatct
      361 gaaaaaatgc aagcgttaaa accacaagtt gaagcagcac aacaaaagct aaaagctgca
      421 acaacacgtg aagaacaaat ggcagcacaa gcagaaatgc agcaagtata tcgcgaaaat
      481 ggattgagta tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc
      541 tctgccttgt attttacggc tcgctacaca gaaggtatcc gtgaatcatc tttttacggc
      601 atcgatctag gctcaccaag tatggtgctt gtggccatcg caggggtcgc ttatctgctt
      661 caaggatata tttcaacaat cggtattcct gaagaacaga aaaagacgat gcgtacgatg
      721 ctgatcgtct ctcctgctat gatcgtcttc atgtcgatca gtgcgccagc cggtgttaca
      781 ctttactggg tagtcggtgg tgtgttcagc tgtctgcaaa cattcatcac aaacgttata
      841 atgaaaccgc gtttgaaagc acaagtggca gaagagttga aacaaaaccc gccaaaacaa
      901 gtcgttactc caagaaaaga cgtgacagaa tcagcaaagc cagctgacaa caaacaagca
      961 acaaaacaaa tcaaacaacc aaaaaacagt acaaacaaca aaggccgcaa cgcaggaaag
     1021 cagcagaagc ggaaataaaa atatatgaca aaaaactgta gacaaataaa aaaatttctt
     1081 tgttctacag tttttttgtt acaacgccgt ttgtataatt aagcgagaca aataaaaaag
     1141 ccatttatgt tacactcaaa ttgtaatacc cgctaaagca tacaaggaga gtgaacataa
     1201 atgacttata cccatcttac accaaacgag cttgtaatga tagaagcata ttttcatcaa
     1261 gaaactccgg ttgctatcgt tgcgaagcag cttaaacgtg gacgccaaac aatttacaat
     1321 gtctataact ttctcaaatg tggtggaaca gcacttgaat actttgaaca atacaaagaa
     1381 aataagcgac gttgtgggag aaccgaaatc attttt
//
LOCUS       DACEPW010000349         1396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.361, whole genome shotgun sequence.
ACCESSION   DACEPW010000349 DACEPW010000000
VERSION     DACEPW010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.361"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1396
                     /locus_tag="IW819_002849"
     rRNA            <1..>1396
                     /locus_tag="IW819_002849"
                     /product="23S ribosomal RNA"
BASE COUNT          375 a          300 c          425 g          296 t
ORIGIN      
        1 agtaagtctg aagaggagtc aaatgcttct tttcttaagg acaagctgtg atggggaggg
       61 aaataatagt accgaagttc ctgatgtcac actgccgaga aaagcttcta gtgagaaaac
      121 agcggcccgt accgcaaacc gacacaggta gtcgaggaga gaatcctaag gtgagcgaga
      181 gaactctcgt taaggaactc ggcaaaatga ccccgtaact tcgggagaag gggtgctgat
      241 catacgatca gccgcagtga ataggcccaa gcgactgttt atcaaaaaca caggtctctg
      301 caaaatcgta agatgaagta taggggctga cgcctgcccg gtgctggaag gttaagagga
      361 gtgcttagcg caagcgaagg tacgaattga agccccagta aacggcggcc gtaactataa
      421 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggcgtaac
      481 gatttgggca ctgtctcaac gagagactcg gtgaaatttt agtacctgtg aagatgcagg
      541 ttacccgcga caggacggaa agaccccatg gagctttact gtagtttgat attgagtgtc
      601 tgtaccgcat gtacaggata ggtaggagcc gtagaaatcg gaacgctagt ttcgatggag
      661 gcgctggtgg gatactaccc ctgcgttatg gccactctaa cccgcaccac taatcgtggt
      721 gggagacagt gtcagatggg cagtttgact ggggcggtcg cctcctaaaa ggtaacggag
      781 gcgcccaaag gttccctcag aatggttgga aatcattcga agagtgtaaa ggcagaaggg
      841 agcttgactg cgagaccaac aagtcgagca gggacgaaag tcgggcttag tgatccggtg
      901 gttccgcatg gaagggccat cgctcaacgg ataaaagcta ccctggggat aacaggctta
      961 tctcccccaa gagtccacat cgacggggag gtttggcacc tcgatgtcgg ctcgtcgcat
     1021 cctggggctg tagtcggtcc caagggttgg gctgttcgcc cattaaagcg gcacgcgagc
     1081 tgggttcaga acgtcgtgag acagttcggt ccctatccgt cgcgggcgtt ggaaatttga
     1141 gaggagctgt ccttagtacg agaggaccgg gatggactta ccgctggtgt accagttgtt
     1201 ctgccaaggg cattgctggg tagctatgta gggaagggat aaacgctgaa agcatctaag
     1261 tgtgaagccc acctcaagat gagatttccc atttctttaa gaaagtaaga tccctgagag
     1321 atgatcaggt agataggtca ggagtggaag tacagtgatg tatggagcgg actgatacta
     1381 atcgatcgag gactta
//
LOCUS       DACEPW010000350         1384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.362, whole genome shotgun sequence.
ACCESSION   DACEPW010000350 DACEPW010000000
VERSION     DACEPW010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.362"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            33..1372
                     /locus_tag="IW819_002850"
     CDS             join(33..474,474..1372)
                     /locus_tag="IW819_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1245924.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          579 a          179 c          240 g          386 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtaaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttgg
//
LOCUS       DACEPW010000351         1384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.363, whole genome shotgun sequence.
ACCESSION   DACEPW010000351 DACEPW010000000
VERSION     DACEPW010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.363"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            23..1270
                     /locus_tag="IW819_002851"
     CDS             23..1270
                     /locus_tag="IW819_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1245925.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKDKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            1285..>1384
                     /locus_tag="IW819_002852"
     CDS             1285..>1384
                     /locus_tag="IW819_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAQ1245926.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVF"
BASE COUNT          498 a          157 c          214 g          515 t
ORIGIN      
        1 tacaaaagct tatctattaa gaatgttcgt gggaaacttt ttcttttttc tcttatttta
       61 tatgactaat actgatctta acaaaagaaa atatcttttt gtatgtttat tgataccact
      121 cacttttttt gttgaattat ttactgatgt ttccgcagct atacctataa tcggaagtta
      181 ttttatatta aaggataaga agaaaactaa tatagtgctt ttaaataaat tactattatg
      241 tatgcttatt gatgatttca gatctattat tacttccatg gttatgacgt atatgttttc
      301 atttgaaggg ataaaagggt atggttatgt agtattccag ataatgcttg attctttgtt
      361 tttgctgatg tttatttggt tatatagaaa attagctata aataggattg ttgaacagtc
      421 cagttctaaa cttactgcta tattcatgat ttatttactt acaattggtt tatttgtttc
      481 ttatgttgct catcaatatc aagtatttga tcattttgtg tttggtgtct taatattttt
      541 aatcattcaa atgtttgttg ttctattttt atttattaga ataactatta ggcaacgaga
      601 acaatatgaa cagcaattga aaagaaaaga attggtttat ctaaaaaaat atactgattc
      661 actggaaaaa gatcaagaaa aatttgccag atttagacat gactataaaa atttattgct
      721 gagtttaaaa gaaatcgcta atcaaaacca tgattcgata ttggaaaaag aaataaaaga
      781 attagagatc tattctaatt cttatctaac caataagttt gaatatcgtt atttaaaaaa
      841 tataaaaaat gaatatttga aaagtttgtt gatagcgaag ctataccaag caaataacaa
      901 taagatttgt tgtaaattcg aatgtccaag tactattatt gaagttcctg ttccaatatt
      961 tgactgtatt agggtgcttg gaattattct cgataatgca attgaagcat ctatagagag
     1021 tcaagataga gaactgtctc tagctattta ccgagatgat aaacagatag aaatttcgat
     1081 atcgaatagt tgtagagaga tactactctc tatagatact ctaatgaaaa aaggaataac
     1141 aacaaaaaag ggacatcaag ggttaggttt atctagtata gaggaaatca ataaaaagaa
     1201 tcccaatatg tttgtgaact atcaaaacga tccgtatcaa tttactactc aagttatttt
     1261 gttgttttga gggaaggagg atttatgagt tatccaataa tattatgtga agatcaaatt
     1321 atacaattaa atcagttaga acgaattatt gataatttca ttctttttca cgataaagta
     1381 ttta
//
LOCUS       DACEPW010000352         1383 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.364, whole genome shotgun sequence.
ACCESSION   DACEPW010000352 DACEPW010000000
VERSION     DACEPW010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.364"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..234
                     /locus_tag="IW819_002853"
     CDS             <1..234
                     /locus_tag="IW819_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1245927.1"
                     /translation="LLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDP
                     RLGVFTLESPSGSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(348..977)
                     /locus_tag="IW819_002854"
     CDS             complement(348..977)
                     /locus_tag="IW819_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1245928.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1111..1183)
                     /locus_tag="IW819_002855"
     tRNA            complement(1111..1183)
                     /locus_tag="IW819_002855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1148..1150),aa:Thr,seq:ggt)
BASE COUNT          489 a          209 c          228 g          457 t
ORIGIN      
        1 ttacttcttc ttgataaaga acagagaaaa aatgattata aactccaatt agatagtgat
       61 attggggatg tttatataaa agattattca aaaatgtaca tggatgaaca tatgaccctt
      121 tctttagatc cacgtctagg tgtatttact cttgaaagcc caagtggttc tttagacagt
      181 catgttcaat tagtagatct acgaccagaa aaattctctg gaataaaaca ataactttct
      241 ctctattgtc cataaaacta gtctcaatca agctgtattg aaaaaacaag acaacattct
      301 taccatggta aataaaaaga cgacacattt tgtcgtcttt ttttaaatta actattttcg
      361 ataaaagatc gcatagcttg tgccattatt tctggctttt ctctattcaa ttcatgtttt
      421 ccgtttttaa taaagagtag ccggccattc gttgaaagtt tatataaacg ttttgctgct
      481 ggtttattcg catgatcttt ggtaccgcat aaaatcaatt taggaagtcg tgatgtacga
      541 agctgttttt tgcagtctaa tgtttcaagt gaacgaagta gggtaagggt ttgtttttta
      601 gatagaccta atttttcaaa tatcttagaa ggtacgcagt aaaaataaat attctggaaa
      661 gtaagcaata agcgattagg agactcaaat tgtggagcga ctaaaacaac cttttttaaa
      721 tatggagaag gagttgcaag atagtttaaa gcaataacag cgcctaaaga taatccacat
      781 aaaataaaag gttcctttat ctgattcaat tgtacttcga tgtatttggt taagtcgtct
      841 aaagtagcag tgtctgtgct gtttaaatga ttgaacaatg aaagactttt gcaagaataa
      901 ctattgggta gataagagat tacatctttc catgattgtt cgtcttggcc tagaccatgc
      961 aaaaaaacaa ttttcatatt gctctccttt ttatagttat catatagact ttctttgtac
     1021 tattaaaata taaacattca tttcttacgt cataaaataa aaaaacagcg cagacacatt
     1081 gttaaaccaa catgttgcac tgtttaagga agccacttgc cgggcttgaa ccggcgacct
     1141 cttccttacc atggaagcgc tctaccagct gagctaaagc ggcattatca ttaatacaaa
     1201 taataggata acgaatatta atgtatttgt caacagaatt ttttcgaaaa aatgtgtttc
     1261 tctaatagga aaacagattt tagaaaaaat aaaaataaca aaaaatgcga acggtttctt
     1321 tgataatata ataaaaaaat tcaaaaaaag tattgcgtta tttagaagaa attgatatta
     1381 tat
//
LOCUS       DACEPW010000353         1378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.365, whole genome shotgun sequence.
ACCESSION   DACEPW010000353 DACEPW010000000
VERSION     DACEPW010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.365"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(154..227)
                     /locus_tag="IW819_002856"
     tRNA            complement(154..227)
                     /locus_tag="IW819_002856"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(191..193),aa:Ile,seq:cat)
     gene            complement(416..1375)
                     /locus_tag="IW819_002857"
     CDS             complement(416..1375)
                     /locus_tag="IW819_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1245929.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          383 a          269 c          260 g          466 t
ORIGIN      
        1 atagggttta aagctgattt taagtgtttt tcgcttatta tcagcttttt attatctaaa
       61 aaaacaactt ttagagacat ccatatactg cttgggggag tcaaaagggg agtcattttt
      121 atattttatt cgcaatttaa atcacttaga gtatgtaccc tgtaggactc gaacctacga
      181 ccggacggtt atgagccgtc tgctctgacc aactgagcta agggtactgg ttgttgccac
      241 ataaagccat aaacaatcaa ccagtagaat gtgtggcaac aaacctgtta tcgcatagct
      301 tggagtgtga ctattatggg tgatagtgaa gatatgcgat aacatcacta ttttatcgaa
      361 tgatttttat agttgtcaat atagttatgt aatgctcctc aacgaggagc tatttttatc
      421 gtttaggaat atttaaatac caacgtttgt catggaaatc ttgcgcaccg cctttagtgt
      481 ttccctctgg atcattcgtt gcccgcatca tgacatagac tttcttatta gggaagttac
      541 gcatgttaaa agatacatga tagccaacat ttccagaagt attataagct tgatttacat
      601 ctgatctata aattccatca gctcttactc gagctaattc tttcccagta ttgtaatcca
      661 taatgaagat atactcgtat ttatagttag caatgtgcca tccagctaca tgcaaatttg
      721 cattttcgat ttctccaaac tgatcaatgt gggcgtaatt cgttccatct gtcagtgtag
      781 gattagctgc accagctcgt gttggatcaa tgacaggctt gttttcagaa gttgttggat
      841 tttcatcggt aaatccatga gctaaatcat atgctaattt ttctttactt acgcccattt
      901 cagaaagata accgtaagga tctgtatgat cgccccagat gttttgtgtt acccataaat
      961 gcgatttgat tcccggttgg ttataaggag tgtccaacgt taatggaata ccatatttca
     1021 ttgctgaatc tctagccaat tcaacgtatg ccttgtagtt tttctcaaac gttgctttat
     1081 catgtgtgtg ttgtaactca atctgcacag gactgttggc attagcatac gaaccagcac
     1141 cgtactgtac ataaccaggt tgaccgactt gataaacaat tccgccgtct cccacaatat
     1201 aagcagtata agcgctagtc catgaacgtt gcatatactg cgcttcattg cgtcctgttg
     1261 ctgtttcatt agccgtttca tgcagtaaaa tatactgatt atttgctact tgtgagctac
     1321 cttcatttgc gcccaaatta aattcattgt tgatagtata ggcaaatccg ttaattgg
//
LOCUS       DACEPW010000354         1373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.366, whole genome shotgun sequence.
ACCESSION   DACEPW010000354 DACEPW010000000
VERSION     DACEPW010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.366"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..813
                     /locus_tag="IW819_002858"
     CDS             <1..813
                     /locus_tag="IW819_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1245930.1"
                     /translation="ITALGTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIA
                     YAIANVATTAVLFYFFKYVLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYL
                     GGIAMMISSYFFFTIAGSSLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGI
                     RNEAVTLSIRPLLDKIAGALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYA
                     PAALMIFSAIIFAWKITLTEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            829..>1373
                     /locus_tag="IW819_002859"
     CDS             829..>1373
                     /locus_tag="IW819_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1245931.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTQDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDAS"
BASE COUNT          387 a          281 c          281 g          424 t
ORIGIN      
        1 atcactgcct tgggcacaaa agaagtcgat actgcgatcc gtcagcaaaa tgaaaaagct
       61 gggatcaaag atgtgttcaa agcagttgct caaaatgatc agttgatgtg gcttgccctt
      121 gcttatatcg cttatgcgat tgccaatgta gcaacaactg ccgtattatt ctacttcttc
      181 aaatatgtac ttggccaacc aactgaattt tggatcgttg gtctagttgc tgccttactt
      241 ggtttagtag ctgttccact attcccaatg cttacacgcg tcatctctcg tcgctacgtt
      301 taccttggag ggatcgcaat gatgatttct tcttactttt tctttacgat cgcaggttca
      361 agtttgccaa tcgttatcat cgggctagta ttgttctact tccctcaaca attgattttc
      421 ttatccgcct tgatgacgat cacagactct gttgaatatg gtcaatggaa aaatggtatc
      481 cgtaacgaag ctgttactct atcaattcgc cctttactag ataaaatcgc tggtgctttg
      541 tctaacggga tcgtagggtt tgtcgctatc gcttctggga tgactggtaa tgctactgct
      601 gctgatatca ctgctcatgg cgtaacgact ttcaaagctt atgcattcta tgctcctgct
      661 gctttgatga ttttctccgc tattattttt gcttggaaga ttacattgac agagaaaaaa
      721 catgcacaaa tcgttgagga attggaaaac aaattggctc cagctgatac aatggaagat
      781 gaacttcacg atgcagtagt tccttcccat taagaaagga tcttgaaaat gaagattaca
      841 gaaaaagatt ttggccaagg ttatcacttg ataaccttgg agaacaaaaa caagctagca
      901 ctttctatct ctgatttagg tgctagaatc gtcagtttaa aatcaaatga tcgagaatta
      961 gttttgggat ttgataccgc tgaagaatat atcgaaaaag atccgtatat cggtgcttct
     1021 atcggtcgca ccgcagggag aatcgaaaat ggccgctttt ctctcaatgg aaaaacttac
     1081 caattagcca cagatccaaa aactgggcat aatctgcatg gtggtgctcc tggatttgaa
     1141 ttaaaaaaat ggtcttatgt cattttgaat ggcgaaaatg aagcttctgt catttttact
     1201 actacaagtc ccgacggaga acatggtttt ccaggtacaa tggatgtgga aatccgctat
     1261 actttgacac aagataatat ctggcgagtg acctctcgcg gcaccagtga ccaagatact
     1321 ttattcaatc cgacaaatca tgtttacttc aacttgacgg gagatgcttc tca
//
LOCUS       DACEPW010000355         1372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.367, whole genome shotgun sequence.
ACCESSION   DACEPW010000355 DACEPW010000000
VERSION     DACEPW010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.367"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            191..445
                     /locus_tag="IW819_002860"
     CDS             191..445
                     /locus_tag="IW819_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1245932.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IW819_002861"
     CDS             446..763
                     /locus_tag="IW819_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1245933.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          492 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat at
//
LOCUS       DACEPW010000356         1359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.368, whole genome shotgun sequence.
ACCESSION   DACEPW010000356 DACEPW010000000
VERSION     DACEPW010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.368"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            20..1261
                     /locus_tag="IW819_002862"
     CDS             20..1261
                     /locus_tag="IW819_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1245934.1"
                     /translation="MNAYQPESVDDMQDALKDVFGPLFEKMLQGELNNHLGYDAHSKE
                     PKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIPKRKRDVSDIEGKVLSMY
                     ARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQARPLAKCYAFLFVDCMY
                     VTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNRWMQVFDELKARGVEDVF
                     FISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSKDYKEVCRDMKKFYGASS
                     LNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSAIRKIMYTTNAVESVHSS
                     FRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAMVLNQLMINETFSSRMKS
                     MRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          416 a          246 c          288 g          409 t
ORIGIN      
        1 aaagttagct gaatccattc tgaatgccta tcaacctgaa tctgtcgatg acatgcaaga
       61 tgctttgaaa gatgtgtttg ggcccctttt tgaaaaaatg cttcaaggag aattgaataa
      121 tcatttaggt tatgatgccc attctaaaga gcctaaggaa cacgataacc gtcgaaatgg
      181 ctatggaact aaaacgctta aaaccagttt tggtgaagta gctattgatg ttcctagaga
      241 ccgggaagct tcctttgaac cagagttaat tcctaagaga aaacgagatg tctccgacat
      301 cgaagggaag gttctttcca tgtatgcacg aggaatgagt caacgagata ttgccgcaac
      361 cgtcgaagct atttatggct ttgatatttc ccatgaaatg atttcagata tcactgacgc
      421 tgtccttcct gaattggaag aatggcaagc ccgcccccta gcaaagtgtt atgcctttct
      481 attcgttgat tgtatgtatg ttactttacg agaaaattat gaagccaaag aatatgctgt
      541 atacaccatt cttggctatg atctcaaagg aaataaggag attttaggat tatggttaaa
      601 tcaaacagaa tctaaaaatc gctggatgca agtatttgat gaactgaaag cacgtggtgt
      661 cgaagatgtc tttttcattt cgatggacgg tgtttctggc ctcgaagaag gcgcaaaagc
      721 gatctttcca tcggtaattg ttcagcgctg tattgttcat cttgttcgaa atgcattgcg
      781 ctatattcca agtaaggact ataaggaagt ctgccgagat atgaaaaagt tctacggtgc
      841 ttcttctcta aacgctgcac atgctgcttt tgacagtttt caaaatcggt ggtctcatta
      901 ttctggtgct gtagatgtct ggaaacggaa ctttgcacat gttgaacaac tatttgatta
      961 tggtagtgcc attcgaaaaa ttatgtacac taccaatgcg gttgaaagtg tccactccag
     1021 cttcagaaaa gtcacaaaaa aaggagcgtt ctctaatgag aatgcactcc taaaactact
     1081 ttatttacgc acgaaagaat tacacgcgaa gtggtcaggt ggtcgaattc aaaactgggc
     1141 tatggtgctt aatcagttaa tgatcaacga gaccttctcc tctagaatga aaagtatgcg
     1201 atttaccttc cgtaacaacg gtaggtttat tcaactatgt gtccgatcaa aatttatctg
     1261 atttcaaaat agccgagaga attatggtgg ttaaaaactc ttaactattt aattacggtt
     1321 ttcatagttt ttggattcag gaggattaaa acgaattct
//
LOCUS       DACEPW010000357         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.369, whole genome shotgun sequence.
ACCESSION   DACEPW010000357 DACEPW010000000
VERSION     DACEPW010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.369"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            53..1306
                     /locus_tag="IW819_002863"
     CDS             53..1306
                     /locus_tag="IW819_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1245935.1"
                     /translation="MTGVSLPLYLIMVLVLIIAMALGKLPTNMIGPIAVLVIFGNLFH
                     FIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPEYVVKSTENFVSNMGFLDFYIAAL
                     ITGSILGMNRNLLMKASVRFIPVALISMVVSFFAVGLVGMLIGNGFANSVLYISLPAM
                     AGGVGAGAVPLSTIYANVLGTDASSIISRLIPATAMTNVLAIIGAALVARAGQSMPKF
                     NGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSLTFFILGQICNYFVPKVHAYAFMI
                     IIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAVLAGIGIALLNLNVLASALTWQFV
                     VLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLCNNSMGGTGNVAVLSAANRMELIA
                     FAQMGNRLGGAIVLIISGFLMQLLS"
BASE COUNT          345 a          257 c          282 g          471 t
ORIGIN      
        1 aactgcaact gcagttgagg aaaagaaaaa agaaggtttt tggtccacaa aaatcaccgg
       61 cgtaagtttg ccactttatt tgatcatggt ccttgtcctg atcattgcaa tggctttagg
      121 gaaattaccg acgaatatga ttggcccaat cgctgttttg gtcattttcg gtaatttatt
      181 tcattttatc ggaacaaaaa tccctatagt caaaagttat ttaggtggtg gctccgtttt
      241 tgcgatcttt gcctctgccg cgattgctac atttgggatc ttacctgagt atgtagtaaa
      301 atccacagaa aactttgtca gcaatatggg ctttttagac ttttatattg ctgccttgat
      361 tactggtagc atccttggta tgaaccgtaa tctattgatg aaagcttctg ttcgttttat
      421 tcccgttgca cttatttcaa tggtcgtctc cttttttgcc gtaggactcg taggtatgct
      481 tatcggaaat ggttttgcaa attccgtttt atatatctca ttgcccgcaa tggctggtgg
      541 tgttggagcc ggagctgtac ctttatcaac gatttatgca aatgttttag gtacggacgc
      601 ttcttctatc atttcccgcc tgattcctgc gacagcaatg acaaatgtcc tagctatcat
      661 tggtgcagcg ttagttgctc gtgcgggaca atcaatgcca aaattcaacg gtaatggtaa
      721 attaatggaa aaaggcgacc ttggcgatgg aaaacctaga aatgtaaaac cttccattcc
      781 acaattaggt gtcggtttga tggtatcatt gactttcttc atcttaggac aaatctgtaa
      841 ctattttgtt cctaaagtcc atgcttacgc attcatgatc attatcgttg ttatctgtaa
      901 aatcgcaaac ctattaccag aatattacga agatgcagct atcatgttca acaacttgat
      961 tgtcaaaaac ttgacagcag ctgttcttgc cggtatcggt attgcgttat tgaatctaaa
     1021 tgtattggct tctgctttga catggcaatt tgttgtctta tgtttgacaa gtgttatcgc
     1081 gatttcagta gtttcaggat ttgtcggtcg tttgtttggt ttatatccaa tcgaatcaac
     1141 tattactgct ggcttatgca acaactcgat gggtggtaca ggaaatgtag ctgtcttatc
     1201 tgcagctaat cggatggaac ttattgcttt tgctcaaatg ggcaatcggc ttggtggtgc
     1261 catcgttttg atcatctcag gtttcttgat gcaattactt tcttagtcaa aaaaaatacc
     1321 tgatcatcaa gtatcggctt gatgaccagg tattt
//
LOCUS       DACEPW010000358         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.370, whole genome shotgun sequence.
ACCESSION   DACEPW010000358 DACEPW010000000
VERSION     DACEPW010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.370"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..429
                     /gene="ssb"
                     /locus_tag="IW819_002864"
     CDS             <1..429
                     /gene="ssb"
                     /locus_tag="IW819_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1245936.1"
                     /translation="DLRYTASGTAVATFTLAVNRNFANQNGNREADFINCVIWRKPAE
                     TMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQLLESKAATESRAHADQ
                     STTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            795..1055
                     /locus_tag="IW819_002865"
     CDS             795..1055
                     /locus_tag="IW819_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245937.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
BASE COUNT          441 a          265 c          267 g          376 t
ORIGIN      
        1 gatttacgtt atactgcaag tggcacagca gtagcgactt ttaccctagc cgtcaaccga
       61 aatttcgcga atcaaaacgg caatcgagaa gccgatttta ttaactgcgt gatttggcgc
      121 aaaccagcag aaacaatggc gacattagct aaaaaaggcg gcttgatcgg tgttgtcgga
      181 cggattcaaa cacgaactta tgacaaccag caaggacaac gtgtgtatgt gactgaagtt
      241 gtagcagata actttcaact attagaaagt aaagcagcga cagagagccg tgcgcacgct
      301 gatcaatcaa ccaccagtcc aagccccact acctttgaac aacgggatac aaacaatgga
      361 ttggacgctt cacaaaatcc atttggtgga caatcaatcg atattagcga tgatgatcta
      421 ccattctaag tagcattttc tattggttgc atttgctacg gtttatgtta taatttagtc
      481 aacaaaaaga attttcaatc tataaccaaa aaccactagc tggcgggcta gtggttttct
      541 tttacgtttc tattataaga attaacgggt aggcttactg tctttcttgg caaatttaca
      601 atcaagccat ttgcagatat agtgcgcggc tacttctgcc atgattgcaa aaacaaaatc
      661 aaaaagaatt ttcaaaccta taacctcctc cccttgccag attaggccgg ggtagcagac
      721 agcaagacta ttatagcata attgaaatat tcaaatgatt ggatttttgc tataatttta
      781 gcgaggtgat tgtgatgaac aaatatgttg gtttgcttga taaaatccga gtaataaaaa
      841 cgcaaccgtt attggttcgc tttactttac aaacgattca tgaatcgatt aattgtgtag
      901 ttgcagatat tgaaattatc gataaattgc taattatgga tgatggaaaa tacaatattg
      961 ccgtcacagg tcacttcaat aaacgaaacc agctagttat tgctagcatg tatgttagaa
     1021 atcccgatca gtttacgcga ttgatgggca tataaaaaaa gaagagttgc aatcctaatg
     1081 taggactgca gctctttttg ttttacatag aaacaagaaa aatgaaaagg cctttttagg
     1141 aaggcgcgag cgccttctca attgaaaaac ctctctcact ccaaccgcca ccgccgttcg
     1201 agaagaaagg aagtgaacgt tgcacgttca ctaagataaa gggtcttgca aagcaagaca
     1261 aaatagaaaa aacttcctct caccaaaagg ttcgttcctg ttcgttatct ctttattgat
     1321 ttgccagacc ggcaacaagt tgcctttta
//
LOCUS       DACEPW010000359         1340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.371, whole genome shotgun sequence.
ACCESSION   DACEPW010000359 DACEPW010000000
VERSION     DACEPW010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.371"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..203
                     /locus_tag="IW819_002866"
     CDS             <1..203
                     /locus_tag="IW819_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1245938.1"
                     /translation="KSMVLFSTTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKF
                     GSINLHPGQERISQTIYRFSAQ"
     gene            225..1244
                     /locus_tag="IW819_002867"
     CDS             225..1244
                     /locus_tag="IW819_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1245939.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT          423 a          286 c          253 g          378 t
ORIGIN      
        1 ataaaagtat ggtattattt tctacgactg gattcgaagc tgatttctct gtcaatggaa
       61 aacagatgca ttcaaactac ggattagcaa tcgaaccgca agaaatccct gatatcgttc
      121 atttccccaa atttggatcg atcaacctcc atcctggtca agaacggatc agtcagacga
      181 tttatcggtt ctctgcacaa taacaagaga aaggtgtttt tatcatggct attaccgtaa
      241 aagatgtggc gaaaaaagcc ggtgttgcca cctcgaccgt ctctcgtgtc atcaacgatc
      301 atcctagtat ttccgagaca acaaagaaga aagtccgaaa aattatggat gaactaggtt
      361 atgttcctaa catgactgcc cgcaatctag gaaaacgcat ttccagtgcc atcggcgtaa
      421 tcttaccccc tcttgattca aaagagcgga tcggtaaccc attttatctt gagatcatgg
      481 aagcaatcaa cgaagaagcg cggaattatg atatgactac agcaatcgct actgctaaaa
      541 attttgaatt gctgttagag aacgtccagc gtatgcattt acaaaaacaa gtagatggtt
      601 ttattctagt gtattcagat agtcaagatc ctgtcattga ttatttatac caaaaccaca
      661 tccctttcac tttgatcgga cagccttatc atcatgagaa cgaaatcgtt tatgtagata
      721 atgataacca gcttctaggt aaacaagcaa cagaattttt gatccagaat ggacataaga
      781 atatcctgtt tgtgacaaac acgactcatg aaagtttgta tttcgaacga tatttcggtt
      841 atcaaaaagc aatgatgatg gctggtcttc ctgcgttatc cagtgtagta ctagagcatc
      901 cggaagatta tctgtccttt gagaaaacgt tgaaagaaaa cgaagccaca gcattggtgg
      961 tcattgacga tatcttcgcc cttcgcacga tgcagttggc gcaaatgtac ggctatgtca
     1021 ttccagagac actctccgtc attagcttca acaactcgat cttctcaaca ttaacacacc
     1081 cttaccttac aagtatcgat atcgatatct ccgaattagg acgtgtcgct atgcaaaagc
     1141 tgcgcgaatt gatccatgaa gaattgagca gtggcgtacg tttggtcatc cctcatcgct
     1201 tgatcaaaag agagacagtc gttcctttag aaaacccata ctaaatagct gactcactaa
     1261 atacgctgac catttatcct atggtcagct ttttatgtaa aagaagatac tacttctaac
     1321 acattgttta tctgtaaaaa
//
LOCUS       DACEPW010000360         1330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.372, whole genome shotgun sequence.
ACCESSION   DACEPW010000360 DACEPW010000000
VERSION     DACEPW010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.372"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..124
                     /locus_tag="IW819_002868"
     CDS             <1..124
                     /locus_tag="IW819_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1245940.1"
                     /translation="RMIWNETGRPGYGLYDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(232..>1330)
                     /locus_tag="IW819_002869"
     CDS             complement(232..>1330)
                     /locus_tag="IW819_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1245941.1"
                     /translation="SNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAF
                     MMNTFFKKEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDLS
                     VDILRPNLPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVE
                     SLCTVFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQT
                     AIEQFYMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYI
                     SKDLIVKIEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDW
                     KKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT          433 a          201 c          225 g          471 t
ORIGIN      
        1 aagaatgatc tggaatgaga caggaagacc aggatacggc ttatatgatc gagcattagg
       61 tgctgtttat ctaaatggat tatgggaagc aatcgaaaaa ggaaacaaaa gtaaagataa
      121 ttagtgaaaa aaactgtaat tttattatgt aaataagaag gatgttcaaa ttcttgatac
      181 ataaatccgg aggttgttaa taagcattcg gatttatgta tcttatttgg tctaggaagt
      241 aacttgattt tcttttttcg attgatccat gagtgcttct tcttttaatt caaagatata
      301 ttctgagatc ctcataatca cttttttcca gtcttctggt gtgatgattc cttttaaaat
      361 atagattttt ttgatatgag aaggcaattg tttcaaaggg cagtcgctga taacgatgtt
      421 gcagttctcc ataaaatcat tttgtatttt gatcgattca tttgaaaaaa tttgttctat
      481 cttaacgatc agatctttac tgatataaaa atcacgcgta tagttcacac caatcacaat
      541 tcgcggctgt tttttgtagc gatcatacaa atgtactagg tcttcaacca tccattgcac
      601 attttccttt tctaaaataa ctggtaacaa gtgttgattt tgttctctga aatccatata
      661 aaattgttca atggcggttt ggacttcttt caatacgcta tgttcttgat aaagcatgaa
      721 agtaggaagg ggcgtatttg gttgattgaa gttaagaaat ccattataaa gtttatttcg
      781 tgcgaatgcc aatgagtaaa gaatcttttc tttttcaggt ataaacaaat caaatacagt
      841 acaaagggac tccaccattt ctcttgaata aagaacatga ggaacgtttg ctttttttaa
      901 aagtttgtat tgctccaagc ttgtttcttc cgattcgatt ttcgtgatag aaagtcgagc
      961 taaaatattc actaagatgc gttgttcgcg acaaattggc agattaggtc gcaaaatatc
     1021 tacagagaga tcaataaaaa agtcaatata ttcatactct gtgaattctt tttgtacgtc
     1081 tttcacagaa gttgcaggaa aattctcttt acatattttt aatagaaaat ataatttatc
     1141 tatcataccg ctatttagtc ggtcaagtac ttctttttta aaaaaagtat tcatcataaa
     1201 agccactact tgcggatctt catcataaaa ttctgtagca gtacttgaaa agatgttcca
     1261 ataaatatct aatgctgcat attgaatttg tacctctttt ccagaaaaag actttctgcc
     1321 attattagaa
//
LOCUS       DACEPW010000361         1323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.373, whole genome shotgun sequence.
ACCESSION   DACEPW010000361 DACEPW010000000
VERSION     DACEPW010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.373"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(328..684)
                     /locus_tag="IW819_002870"
     CDS             complement(328..684)
                     /locus_tag="IW819_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1245942.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(698..1108)
                     /locus_tag="IW819_002871"
     CDS             complement(698..1108)
                     /locus_tag="IW819_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1245943.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          432 a          241 c          184 g          466 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcatttc caacatgtca acaactattt tatatgtttt
       61 ttaaaatact attttattgc tttgctactt aaaacaacag aaatgaatat atcatgtaaa
      121 tttacacatg tcaatatatt tttaactcta tatccccacc tagatttgaa ttttgaccac
      181 ataaattgag catgtctcta taaattgagc atgtctctat agtagaaaaa taaagtcaaa
      241 aaaagaacac ataacacgct tgatgctaag tgttcgactg ttacaactgt atgaaatgct
      301 cagtgacttg attaaaacag ttaaagtcta tttatttaag ataagctgtt ccatcaactg
      361 ctcattgtat ccaataaata gaacttcatc gataataatc aaaggacgtc tgaggagatt
      421 ggtatgttgt gtgcataaaa aaatcagttg tttttttcct acacaggaaa ggtcaatcgc
      481 aatgttgtga aaagcctcag accaagtcgc aaatagctgt tctgcttgca cttcttcaaa
      541 actgcataat tcttctgctt cttctgtact tagatcgcgt tcttctaagt caacaaattg
      601 ataaggcaat tgatggtgtt ctaaccattg tttcgccttc agacttgtta cacagtgcgc
      661 tgatccatag actttcactt ccacgaaggc tccctcccta tgaatttaag atgttattgt
      721 tttgtaaata gatcagattt ctggcaacac atcgaatttc acgagggaca aatgaccgga
      781 tctcatcttc attaaatccg attagtattc ttttttcatc aatcaaaata ggtcgtctca
      841 ataatgaagg atattctttg aataagacga tcatttgctt taaagttaat tcgttaaaat
      901 ctatgcccag cttttcgtaa attttcgacc gagtggaaat aatatcttga actcctcctt
      961 cagtcgctgc caagatcttt ttgatctcac tttctgttaa cggttcggca aaaatattct
     1021 tctcttcaaa ttcaagttga ttcgcttgca accatgctct tgcttttcta caggaagtac
     1081 agctaggcga agtatatact tttatcattt tctcactcct ttttaattat aatccaattc
     1141 tacagtaatt tcttttaaaa taccaattat tagctaataa aatataaaaa aaaaaaaaat
     1201 caaaataact gattatttac gtttgaataa tgagaattat tctatatatt ctctattttg
     1261 ataatagtat tctcattttt aaaataaata agggaggtgt gacaaaattc ttgtcacacc
     1321 tcc
//
LOCUS       DACEPW010000362         1317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.374, whole genome shotgun sequence.
ACCESSION   DACEPW010000362 DACEPW010000000
VERSION     DACEPW010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.374"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..>1317
                     /locus_tag="IW819_002872"
     CDS             100..>1317
                     /locus_tag="IW819_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1245944.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNA"
BASE COUNT          430 a          271 c          240 g          376 t
ORIGIN      
        1 tgacaccagc atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta
       61 gaattaattt ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc
      121 aaagaaacat tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa
      181 gaaaaataca aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa
      241 gaatgcatcc actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc
      301 gtccggttgt taatgaacga tgtttctgaa taccgtacct acctcgaatt gaaaaagcaa
      361 cgcttcaaat gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa
      421 cattgtttca tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc
      481 tcaatgacag agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg
      541 aagcgatttt atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt
      601 gatgaattca aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga
      661 caaacacagc gcttattgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat
      721 tttagtcatt tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac
      781 gctccttatg tatcgctcgt taaaaagctg tttccaaaag cacagctgat tattgaccgg
      841 tttcacatcg tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac
      901 caactattaa aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac
      961 tggaaacttc ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct
     1021 ggcttcaaag ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct
     1081 gctttgcgtg taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac
     1141 tacgtatcgt ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg
     1201 accttgagaa cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat
     1261 tcaaatggcc cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgca
//
LOCUS       DACEPW010000363         1304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.375, whole genome shotgun sequence.
ACCESSION   DACEPW010000363 DACEPW010000000
VERSION     DACEPW010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.375"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(146..1129)
                     /locus_tag="IW819_002873"
     CDS             complement(146..1129)
                     /locus_tag="IW819_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1245945.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          367 a          281 c          246 g          410 t
ORIGIN      
        1 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
       61 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
      121 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
      181 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
      241 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
      301 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
      361 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
      421 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
      481 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
      541 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
      601 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
      661 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
      721 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
      781 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgatg tcgattcctt
      841 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
      901 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
      961 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     1021 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     1081 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     1141 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     1201 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     1261 gactgtgaca aaaatcttat cacagtccat tttccggaat aaat
//
LOCUS       DACEPW010000364         1298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.376, whole genome shotgun sequence.
ACCESSION   DACEPW010000364 DACEPW010000000
VERSION     DACEPW010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.376"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            125..700
                     /locus_tag="IW819_002874"
     CDS             125..700
                     /locus_tag="IW819_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1245946.1"
                     /translation="MCGRYFFDLESELLQNYYREAQKKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLIINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(905..1243)
                     /locus_tag="IW819_002875"
     CDS             complement(905..1243)
                     /locus_tag="IW819_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245947.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
BASE COUNT          422 a          224 c          255 g          397 t
ORIGIN      
        1 accattcact ggagagtttg tcacttgtca caactttttt agttctacac aaattgaaag
       61 cgttttgttg gtgcgaactt cttgttgcag tgtatgatag aaaaaaaaat tgggagggat
      121 taggatgtgt ggtcgttatt tttttgatct agagtcagag ctccttcaga attattatcg
      181 agaagcccag aagaaacaac cggagcaggc agcagaatta gcagctggag aagtttttcc
      241 ttcaaatctt gttgttacgt taagaaaaga ggaagaaaac atcctcactc cggaaatcat
      301 gaagtgggga tttaccggat ttaaaaaagg acaattgata atcaatgccc gtgcagagac
      361 agtagaagaa aaaaagacct ttcatcaacc atttttgcaa aatcgttgtg ttttcccaat
      421 gagtggcttt tatgagtgga atgaagaaaa agacaaattt atgttttcaa ataaggaaaa
      481 aatagtgtat attggcggtt tttatcgtag ccatcaggat gaaaaaatgg cagaatcaat
      541 cataatgact actgaaccta accgcacagt cgcaccaatc catgaccgaa tgccgcttgt
      601 cttgactgaa gaacaaatcg aaccttgggt aacagatatt tcttttgcgc gaaaaatcat
      661 tactcagcag atgcctgaat tagtaatgga gaaggtataa cataatcgat ttttcaaaga
      721 aagagcggca aaaaacgaaa aagacttcag cacagatccc aaaaacaaag agaacagaag
      781 aaatgctcat aagctgcata tactgcatct atagggtcac agtcgacctt tctaatgtat
      841 agcttctatt tgttttttac tcgtgctgcg agtctttttt atttatacct aacgatgttt
      901 atcattatga ctctttgaat tcttggaatc taaaattcaa agttaacgac tggttggcag
      961 ttgtatattc tctgaattta tagataagag gttgccattt atccacattg atatgagttg
     1021 gatcatgtaa aatcccttct tctacaaaaa tagctacgat ctcacgttct acaatagcaa
     1081 agtcttgtct agttgaaatt cgttgaacca atgtttctat ttgaagcggg cattctttga
     1141 tccgaacagt tgaaacttct tcacctgcta gtttcgtgaa ctttcccaat tgaaatttgt
     1201 cagagcaaaa ggaatagcca gctttttgtt tataatcggg catgatagga tggcctgttg
     1261 tctttgaaat tttctcccaa agcgttttat ctgcaaaa
//
LOCUS       DACEPW010000365         1296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.377, whole genome shotgun sequence.
ACCESSION   DACEPW010000365 DACEPW010000000
VERSION     DACEPW010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.377"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            279..884
                     /locus_tag="IW819_002876"
     CDS             279..884
                     /locus_tag="IW819_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1245948.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            1019..>1296
                     /locus_tag="IW819_002877"
     CDS             1019..>1296
                     /locus_tag="IW819_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1245949.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTK"
BASE COUNT          481 a          201 c          256 g          358 t
ORIGIN      
        1 tggggaggtt gtgaaaaagc tgtcctgcct caattaataa gaacagccag acaagaatag
       61 ctcgtcatat ttttcgtttg gtggggctta ttaccgcaga tgcaagcttt tgaacgccat
      121 ttattcacag ccccagagaa gtcgaatgtc ttttgttctc gagtttttta acattctttt
      181 atttattgca gatttcatta tatttatttg ccgtaattaa accagtcttc tatacttgat
      241 tacatagtca tacatttgct aaaaaagaag ggaagagaat gaacaagaag ttggctcaat
      301 acaaaagact actcgcactt tctcaagctg gtctatatta tggaaaagat gtatttgata
      361 aagagcgttt tgaggagatc cggacgattt cattggaact gatcagtgaa ataggaaaag
      421 aaactttcga agaaataaaa gcgctcacaa cgattggaga aggacatcca acaccaaaag
      481 tagatgttcg tgcctatatc aaaaaagatg gcaaggtcct actaatcgaa gataaacgga
      541 cgaaagaatg gtcacttcct ggtggatttg ctgaaatagg attgtctcct gaagaaaatg
      601 tgcgtaaaga agtctatgaa gaaacaggat taacagtaga aactactcag ttgcgagcag
      661 tttttgatac gaataaacaa aaagatattc cacagctttt tcagtattat aaacttgttt
      721 ttgcttgtgc gatccacggt gaagaggcca agtttattga gaataatgaa acttccaaca
      781 tgggattttt tagtatagaa gagttgccaa agctatcaga aaagcgaaca acgaagaaac
      841 agttacttat tttagaaaac aaaaaccaga tctattgtga ttgagctttt tcgtaaaaga
      901 agaaaagaat atactttact agttattaaa aaagaacttg atttttttat tgaaaatagt
      961 tgcatttgca acaaaaagag gtaaactagt cttacaaaca aaataaaggt gggatgaaat
     1021 ggctgaaatc cttcgagaca tcggaatgat tgcccgagcg ctagattcta ttagcaatat
     1081 cgaattcaaa gaagtcgatt taacaagagg acagtatctt tatttagtac gtatttgcga
     1141 aaatcctgga atcatccaag aaaaactagc agaaatgatc aaagtagatc gtacaacgac
     1201 tgcccgagca atcaagaaat tagaatccaa tgggatgatc gaacgtttgg aagataaaga
     1261 aaataaaaaa atcaagaaac tctatccgac taaaaa
//
LOCUS       DACEPW010000366         1280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.378, whole genome shotgun sequence.
ACCESSION   DACEPW010000366 DACEPW010000000
VERSION     DACEPW010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.378"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(19..738)
                     /locus_tag="IW819_002878"
     CDS             complement(19..738)
                     /locus_tag="IW819_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1245950.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            1075..>1280
                     /locus_tag="IW819_002879"
     CDS             1075..>1280
                     /locus_tag="IW819_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245951.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEK"
BASE COUNT          424 a          214 c          276 g          366 t
ORIGIN      
        1 accccctcag aaagtctatt aaaattcatt cacaaagaaa tcgaataagc gctgttcaga
       61 tggatgtaca cggtgggtaa gttccggatg ccattgaaca ccgaggattt tatgcatatc
      121 atctttgctt tgaaccgctt caacgagtcc atcgttagaa aaagcgatgg cttttaataa
      181 aggtgaaaga tctttgactg cctgatgatg gtaagaattc acaaaatatg gatcggggag
      241 aaggttgctt aacaaactgt ctgaaacaat cttgacttcg tgagtcgtaa attgcggggc
      301 agtgggctgt tgttcatgtt ttactgacca ctttggatat aatgaaagat cttgataaag
      361 tgtaccttcc aaaacgacat tcaatagttg gagtccccgg cagactgcaa aaattggttt
      421 tctttgtttc aatgcttcca agatcagtgc ctgttcaaat tggtctcgtt gaatatttgt
      481 ttccgttagc tttggatgag gatcttccat gtaaagttgt ggactgacat cttgaccacc
      541 tgcaagaata agtttgtcga tttgtccaat cagttgagga gctgaatctg gagacatgac
      601 tggcagaata agaggcaatc cttgagcatc taaaactgca tccacgaatc cttgcggtgt
      661 atagctcact tgatttcctt ggaaagtatc tgttgctcga atcagttgat ttccggcaat
      721 gccgatgatt ggtttcattt gtattccccc taaagtagac ttattgtaac aaggatttaa
      781 aaaaaattaa actatgtaag acttgcccct atacttatct gttcttgctg tgttactatc
      841 tgtaaagaga aatttcgtgt gaaattcatg acgatagttg aaaatattgc ttaatttgtt
      901 ataataaaaa gtaagcagaa ataaaaaggt tgggcgtaag cttcaaggat ctagttcttc
      961 aaggggtgag aaggacattt tattgaaggg gcctgcctat actaagttat ctttagaggt
     1021 acatactttc aatgaaaaaa gaaagcatgt acctcttttt taaatcgaaa ggaagtgaag
     1081 caagtgttag aagctttgga acaaatccga aaagcagaag aagaaaatga aaagcgtgct
     1141 caaacactgc ggcagcagct gaagatctat gcaacagaaa aagagcagga attggaaacg
     1201 atccaaacaa actatcgaca acaactaaaa cagttggtgg aagaaaaaga acaatttgaa
     1261 atacaagaat tagaaaaaga
//
LOCUS       DACEPW010000367         1280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.379, whole genome shotgun sequence.
ACCESSION   DACEPW010000367 DACEPW010000000
VERSION     DACEPW010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.379"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..859
                     /locus_tag="IW819_002880"
     CDS             <1..859
                     /locus_tag="IW819_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ1245952.1"
                     /translation="RAPEIKQEVLMEIFTQLVARISAQQPSSKTTSLQLIDSTTIPLN
                     KAWFPWAKFRKTKSGIKLHLNLCYLDKTNQYPESFTMTNASEHDRNHLEVLVDKTQAT
                     YVMDRGYFDYKLLDKLNRDGYFFVTRTKSNTKITILDQIEVADTTTRDGTIISDQQVI
                     LVGGVNHVTERFRLVTVLTKGQKILRMVTNLFDVSPNEVADMYQARWQIELLFKHLKQ
                     NLTIKRLYSHSEQGAINQVILTLIATLLTYVIKIELNTTATLFQLKRTFHYLMFEPAE
                     EWFKRYKPG"
BASE COUNT          417 a          248 c          238 g          377 t
ORIGIN      
        1 tagagcgcca gaaatcaagc aagaagtatt gatggaaatt tttacgcaat tagtcgccag
       61 aatttctgct cagcagccaa gctcaaaaac aaccagtcta caattgattg attcaacgac
      121 tattccgttg aacaaagcat ggtttccatg ggccaagttt cggaaaacaa agtctggcat
      181 caagctacat ttgaatctct gttatcttga taaaaccaat cagtatcctg aaagttttac
      241 gatgacaaat gcgagtgagc atgaccgtaa tcaccttgaa gttcttgttg ataaaacgca
      301 agcaacgtat gtgatggatc gcggctactt tgattacaag ttgttagaca aactaaatcg
      361 tgacggctat ttcttcgtta cacggacaaa atctaataca aaaattacca tcttggatca
      421 aattgaagta gcagatacaa caacccgtga tgggacgata atcagtgacc aacaagtgat
      481 tcttgtcggc ggtgtcaacc atgtgacaga gcgttttagg ttggtaacgg ttcttaccaa
      541 gggacaaaaa atcctgcgaa tggtgaccaa tctctttgat gtatcgccaa acgaagtggc
      601 cgacatgtat caagcccgtt ggcagattga attgttgttc aaacacttaa aacaaaatct
      661 gaccattaaa cgactatatt cacatagtga acaaggggcg ataaatcaag tcatcctcac
      721 cttaatcgca acgcttctca cttatgtgat aaaaattgag ttgaatacga cagcgacgct
      781 gtttcaattg aaacgtacct tccattactt aatgtttgag cccgcagaag agtggttcaa
      841 acgttacaaa cctggatgag gatgtagtta atagctgttg tcacgtttga tttttgaata
      901 catactaaaa ttttctggaa aatagtcatc cgctgcgtca gcacttgact tttttgaaat
      961 ttttagcaag aaataaatgt cagactattc tgacgatatg ttcgcaacat tagtgatact
     1021 aaatagattt ttcgctatct ttttataact tcacatatgt ccgaaaagcc ttattttaca
     1081 ggctttccgg acatattcac tatcttgctt acctcaatcc taataaaaca ttcttttcaa
     1141 catcagtgag catagcaggt catagatatc ttaatccttt tgcaagtatc ttttcaaaag
     1201 ctgttgttgt atactgaaat ccgtattgtt acaggaatac tgaagttgtg acaaaaacat
     1261 agccatcaag gaaaaggtat
//
LOCUS       DACEPW010000368         1279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.380, whole genome shotgun sequence.
ACCESSION   DACEPW010000368 DACEPW010000000
VERSION     DACEPW010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.380"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            354..686
                     /locus_tag="IW819_002881"
     CDS             354..686
                     /locus_tag="IW819_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1245953.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            982..1137
                     /locus_tag="IW819_002882"
     CDS             982..1137
                     /locus_tag="IW819_002882"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1245954.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          459 a          226 c          229 g          365 t
ORIGIN      
        1 aattcgtttt tagcttaaaa ataacttttg tcacagtctc actcctaaaa ttactttatt
       61 tacgtatgaa gtgggcagga ggtcgaatcc agaactgggc tatggtactt caacgaggac
      121 ttctcctcta gaattgaaaa gcatgcgatt taccttccgt aacgacggta gatttattca
      181 actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat tttggttgtt
      241 aaaaactctt atctatcaac ttccacagtt tagttgacaa accctgatac taagttgggc
      301 tatgatacac ttttcttgac tatcagttac gttaccggaa ggaatgaact ctattgagaa
      361 aaacatatga tcgtaaattt aaactaaaga tttcacagga tatccttgag aaaaaagtaa
      421 ctaccaagaa aattgctgaa gaatacaata tctctcgtcc aacaatttcc cgatgggttt
      481 cagaatatcg tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga ttacctgata
      541 aagcagattt ctacattctt gaacaggaaa ataaacgact aaaagaggaa gacgagtgtt
      601 ctacgaacac aaagatcgat acggatctcc agggatcact caaaaacttt acgatgaagg
      661 aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt tatgcacaag
      721 aggatttcat cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag aaatggtaaa
      781 aaaatgttga atcgataatt tgaacagaac caaattgatg ctgtttgggt gatggatatt
      841 acttacattc catgtaatga cggtcgcctc tatttgtcca cctatattga cttagcaact
      901 cgtattctac gttactatat ggtagattca cacatgaaaa aagagattgt cgtccgtcca
      961 ttacaaattt attaaggaga attgcctacg gtaattcatt ccgatagagg tagccagcat
     1021 cggtctttta tttatcaaga gctgttgaac gaccaccaca tcacacattc agtgagccaa
     1081 actgggactc ctgttgacaa tgcagtaatt gaagcgtttc atcgatcaat caaatgagaa
     1141 ttaatcgact ccaacaaata taaaagtaaa atagagatga aggtgttaat ccaagattat
     1201 ttagaagact actacccaaa caaaagaatc catacaaaat ttatgatgac tccttgacaa
     1261 tttgaagcga agaaaataa
//
LOCUS       DACEPW010000369         1273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.381, whole genome shotgun sequence.
ACCESSION   DACEPW010000369 DACEPW010000000
VERSION     DACEPW010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.381"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            11..898
                     /locus_tag="IW819_002883"
     CDS             11..898
                     /locus_tag="IW819_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1245955.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            984..1214
                     /locus_tag="IW819_002884"
     CDS             984..1214
                     /locus_tag="IW819_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1245956.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
BASE COUNT          465 a          207 c          282 g          319 t
ORIGIN      
        1 ggtcgaaaaa atgaaacttg gttttatcgg aacaggcgtc atgggaagtg ctgtcgcacg
       61 ccatctacta gaagcaggac acgaagtagc tgtctataat cggacaaaag caaaagctga
      121 tcctttagta acagaaggag ctatctgggc agatacacca aaagcggtag cagaacaaag
      181 taatattctc tttacaatgg taggttaccc caaagatgta gaagaaatct actatggaca
      241 gtcggggatt ttttctgcag atatctcagg acatatttta gttgatttga ctacaagtac
      301 accaagcctg gcagaaaaaa ttgcaaaaac agcaaaagaa aaaggcgcag atgcgttaga
      361 tgcaccggta tccggtggtg acttaggagc aaagaatggt acactgacga tcatggtcgg
      421 tggagaagaa gccgtttatg atcaagtact gcctttattc aaagaatttg gtacgacatt
      481 tacattgcat ggttctgctg ggaaaggtca gcatacaaaa atggctaatc agatcatgat
      541 tgcaggtacg atgacaggaa tgacggaaat gcttgtctat gcacaaaaaa acggacttga
      601 tttaaagaaa gtgattgaga cgctttcagg tggaagtgca gctaactggt ctttaagcaa
      661 ttattctcct cgtattttaa aagaagatta cacacctggt ttttttgtca aacattttat
      721 caaagattta aagattgcat tagaagaagc agaaaaaatg gatcttgtat tgccagcaac
      781 tacccaagca ttgaaactat atgaagagtt ggcagataaa ggttttgaga atgacggaac
      841 ccaagctttg atcaaacttt ggtggcctga aggaaaaata ccagaaaaaa aatcataaaa
      901 aacgagtttt tttagagaaa atcaatgcaa gtaggctatg cttttgatac aatataacag
      961 aaataagaaa aggaggacct ccgatgacac attcacaatt agtcgcaatc ataaaacgac
     1021 tggaggctat ggtcgagtca gcagataatg aactgcaagt tcgccgtttt gaaaaagaag
     1081 gcgtagagaa gtgtacagta acttatgata aagcgaccga tacatttgaa ttaactgaaa
     1141 cagatactca tcagcaatac gaatttgata atatcgatat cgtagcgatg gaaatctatg
     1201 acttaatcca ataaatttta ggataattga catttgaagt tgaagatgcc taaaagaagc
     1261 cgagaagcaa cta
//
LOCUS       DACEPW010000370         1264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.382, whole genome shotgun sequence.
ACCESSION   DACEPW010000370 DACEPW010000000
VERSION     DACEPW010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.382"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..465
                     /locus_tag="IW819_002885"
     CDS             <1..465
                     /locus_tag="IW819_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245957.1"
                     /translation="TQNNDQLVSGNFTLKGEGQLTFYLLPTIAEMKTAIRRKDKIQPT
                     VTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSERDSPSRILKRIKFPPYLEQI
                     VVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLHANKRPKKIAATKK"
     gene            480..1043
                     /locus_tag="IW819_002886"
     CDS             480..1043
                     /locus_tag="IW819_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1245958.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLIDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
BASE COUNT          434 a          211 c          236 g          383 t
ORIGIN      
        1 actcaaaaca atgatcaact ggtatctggc aacttcacac taaaaggtga aggacagttg
       61 actttctatc tattgccgac gatagctgaa atgaaaacag caattcgccg caaagacaaa
      121 atccaaccaa ccgtgacaat gagcagaaaa acatctgata agattcaagg tgtggctttt
      181 tatggccatt atctcttagc cagtcgttct tatggtcctt atacctcgga actcttagtg
      241 tccgagcggg actctcctag ccggatccta aaacgaatca aatttccacc gtatttggaa
      301 caaatcgttg ttgtagaaga tcgtctagca gtattgtttg aaagtggcgc cgcagcgtat
      361 cgcgagaaag ccaatcctgt gttagccaat gttttattac tagacttagc aacgttgctt
      421 catgcaaata agcgtcccaa aaagattgct gcaactaaaa aatagtcgga caactcaaga
      481 tgatcactgt tgttcataaa gaacgacaag aaaaatcatc tagcgatgtt cgactatggg
      541 tttatttagg aagtatttct actaagattc aatatggcta tacagattcg gcgaaaaaac
      601 aaggcaatgt taagtttttg agaatcacag atatacaaga gggacgtgta aattggtcca
      661 gtgtgcctta ctgtgatatt agtaattcta aactaattga tttaagatta gaagaaaatg
      721 acattttgat agcaaggaca ggaggaacta tgggaaaaag tttcttggtt aaggaaataa
      781 gtgaggaatc tgtttttgct tcttacttga ttcgaattag gttagttgag aaattattat
      841 cagaatacgt agactgcttc ttagactcgc cattatattg gaagttatta gaaaaaattt
      901 cttacggaac tgggcaacca aatgtaaatg gtacaaattt atctaaacta cttataccat
      961 taccaccttt agaagaacaa caacgcatga ctactaaaat agaaatgata agaagatcta
     1021 ttcgtagaat caattttgag taaatataac ggacctagca tccattttat accagatgat
     1081 gctaggtcta ttatttatat tttatggaat ttaattgatt tgttctagtg ttttaatatt
     1141 attacgacag taaaaaaata gtttgattct tctaacatga atgaccatct agaagtggaa
     1201 atgtataaaa gatttacttt atgaagtttt cctaatatca ttcactcatg atacgcagcg
     1261 cctt
//
LOCUS       DACEPW010000371         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.383, whole genome shotgun sequence.
ACCESSION   DACEPW010000371 DACEPW010000000
VERSION     DACEPW010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.383"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            198..740
                     /locus_tag="IW819_002887"
     CDS             198..740
                     /locus_tag="IW819_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245959.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            830..>1246
                     /locus_tag="IW819_002888"
     CDS             830..>1246
                     /locus_tag="IW819_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ1245960.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMPD"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 tgatgaagca gatcccaaag ggctgcttca tcaatttttt cgtaagtgtc cgtacactta
       61 ctgtgcttgc tcatatatta atcgatcata tactaatcgt gtaagaacaa ctttaagcat
      121 aatacttata ttgttatacc atagtttgga aaaataaaaa attatatttc atcaacgttt
      181 cataaaaggt gtgtagaatg ttattgaaag tagctataaa atgctattgt gtaaaaaata
      241 aaggaggaat aatttatgaa aaaatagtac caagaggagc atgcctatta cttgcattat
      301 gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga aaacaataaa ataaaagaag
      361 gacaatccat aagttctgaa gaggaaggca tgtcaattaa tgcaaatggc aaaaatgaaa
      421 cttttaagcc atcagattat acattggagg caaagaaaga atatgtttat gaatatttag
      481 ggcttaaatt taaactctca gacaaattta gaaattatat agctgataaa aagatagcga
      541 tgttagatga tcaaagtcct atagataaag aactaaagta tgctattttg acatttgaga
      601 aaatgacaga agaacaaaag aatgcagtta tagaaaagat gggcgatgga tataaaaatt
      661 ggcaaaatga gcttgaaaga attgggacta ttggtatatt tgaaaaaaat acatcagaag
      721 aaaaaaatct aaaattataa aatgtgatac tcatactaaa ataggagttt caagtgatgg
      781 aaaatacgat tgctatttaa gcacaaatag cggagctgaa agtaatcttc tggatgaatt
      841 aaaaagaact gagattcaaa ttatagataa aaaagaacgt cctaaaaatg gttttgtgct
      901 atcagaaaaa actgatttag aaaatacaga agcatttaac aaagaatcgg ttaaagattt
      961 acgtaaacta ccaacaaagg acataaatgg caaggatttt acaagtaaag attttgagaa
     1021 gtatgatctt actatggtaa atgtatttgc aacttggtgt acagcttgtg taaaggaaat
     1081 tcctgatttg gttgaggttc agaatgaaat gaaaagcaag ggtgtaaata tcgttggagt
     1141 tgttacagat cctgttgatg ataacggtga aaacaaggaa gcaattgaaa agtcaaagtt
     1201 aatacatgaa aagacaaagg cttcatatcc tttcttaatg ccagat
//
LOCUS       DACEPW010000372         1227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.384, whole genome shotgun sequence.
ACCESSION   DACEPW010000372 DACEPW010000000
VERSION     DACEPW010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.384"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..434
                     /locus_tag="IW819_002889"
     CDS             <1..434
                     /locus_tag="IW819_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1245961.1"
                     /translation="HDHKWQFGGAGEHMMLEQQMFARKSQYEPVEEGSGADFIEELKP
                     YLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRELVESGFN
                     VVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            627..1208
                     /locus_tag="IW819_002890"
     CDS             627..1208
                     /locus_tag="IW819_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1245962.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASETGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
BASE COUNT          429 a          186 c          278 g          334 t
ORIGIN      
        1 cacatgacca taaatggcaa tttggcggtg caggagaaca tatgatgctt gagcagcaga
       61 tgtttgctag aaaaagtcaa tatgaaccag tcgaagaagg ttcaggagcc gattttatcg
      121 aagaattgaa accatatttg gatgaaaata cgatcatcag ctctcctcat aaaatctttg
      181 gaccagaaag caatgatttg gcgcttcaat tgagaaaaaa tggaatcgac acagtaattc
      241 tcggtggaat gaacggaaac ttgtgtagcg attcccatat gcgtgaattg gtagagagtg
      301 gattcaatgt tgttgtagcg acagatgcaa ttggtgctcc tggccaagaa gcaatggatg
      361 cagcgttgac gaactttggc tttattgcag atcaagtgat gacaacagag gaagtattag
      421 agcaactatc ataagatagg aactgctttt gtcctcgcag ttcttgaatc aatgaacatc
      481 tagctatatg agaacacatg agaacagttt tatagtgagg gctgtgtcag ataatttggc
      541 acagtctttt ttaaacgatt aagaagaaaa aatgatgaat ttattgtata atataggact
      601 atgagaaatg aaaaaggaga atatcattga aaacaaagga aaagatcttg gaagcgacca
      661 aagatcttat ttataaaaaa ggctatcatg aaacgtcaat ccgagatatt ctagcagctt
      721 ctgagacggg gaaagggcaa ttttattatt acttttcttc aaaaaaagaa gtttgtctag
      781 cggtgatcca tgcgcatatg gacgtgtggc agacagaatt gttcgaagac atcttaaatg
      841 ctggaaaaca gccagaagaa gcattagaag aaatgttgga ctggattttt gctgatcatg
      901 agacacagat cacttatcat ggctgtcctg taggtaattt gattattgag ttgagtacag
      961 tggatgaaga ataccgtctg ttgctactag aattagtcaa tcaatgggtc aagtatacga
     1021 aacaaaatat cctggcactt tctgaagaaa aactgtccga tgaagaagca gaccgtcaag
     1081 ccaaagcgtt gattgctgaa atccagggaa gtatcttatt gttgaaagtc acacaggatc
     1141 tatcactact tagacaaaat tttcaattga tcaaagaaag attgttaact tcgcaaaagg
     1201 ttctttaata attagaggtt gtgaaaa
//
LOCUS       DACEPW010000373         1208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.385, whole genome shotgun sequence.
ACCESSION   DACEPW010000373 DACEPW010000000
VERSION     DACEPW010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.385"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..231
                     /locus_tag="IW819_002891"
     CDS             <1..231
                     /locus_tag="IW819_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245963.1"
                     /translation="DLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYLL
                     KRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(390..512)
                     /locus_tag="IW819_002892"
     CDS             complement(390..512)
                     /locus_tag="IW819_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1245964.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            702..926
                     /locus_tag="IW819_002893"
     CDS             702..926
                     /locus_tag="IW819_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245965.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
BASE COUNT          394 a          227 c          226 g          361 t
ORIGIN      
        1 gatctattga tacccgtaat tgcatttata gacgggattt ctttgggcgt cactgaaaat
       61 cctttggtaa atagaattgc gaaaggatca ctatttcttg tattcgtgta tcttgtctgt
      121 tcttatcttt taaaaagagc aggaaaggat aattcctttt ggcgattgac taaagtcaca
      181 gtagcagcga cagctgttat acttacggct attctaccgc tttatatgta gagttaacgg
      241 gaaatcgttg aacttcttgc gaaatattgc tataatgaac atgaaaaaga ggagttgcgc
      301 taacaacccc tcattaacag caccgattaa agaacggtgg cagaatatta gttatttgga
      361 aataaccgct gacctgctaa agtagacggt tatttctttt tgtcattttt aagcatattt
      421 acaatcaaac taatcaaagc aatggtaaac ataccaaaac caagaatggt ttgaattgtc
      481 tcatatgcgg acaaaaggct tactcctttc atagatttta gcacttacat tcataagcac
      541 caccaccttt ccagaagtcg ccaccgtctt caaactttac tgtcttgact attatagcat
      601 atctaaaatc gaataaaaat tctcaatttt agctgttttt gctataattt tagcgaggtg
      661 atcgtgatgg aacaatatat tggtttactc gataaattag ggtgatcaag acacagccat
      721 tgcttgtgcg atttacatta cgcacgagtc atgaaccaat caattgtgta gtggccaata
      781 tagaaatagc ggataaattg ctgattttgg aagaaggcaa gtatactatt tcagtgacag
      841 gtcacttcaa taaacgaaag caactagtca ttgaatcaat gcaaattaga aaccctgatc
      901 attttacgcg atcgatgagc atataaaaga agagctgcag tcctaaatga ggattgtagc
      961 tctttttgtt ttacatagaa acagtaaaaa tgaaaaggcc tgtttaggaa ggcacaagcg
     1021 ccttctcaat tgaaagacct ttctctcact ccaaccgcca ccgccgttcg agaagaaagg
     1081 aagtgaacgc tacgcgttca ctaagataaa gggtcttgca tagcaagaca aaatagaaaa
     1141 aatttcctct caccaaaagg ttcgctcctg ttcgttatct ctttattgat ttgtcagacc
     1201 ggcaacaa
//
LOCUS       DACEPW010000374         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.387, whole genome shotgun sequence.
ACCESSION   DACEPW010000374 DACEPW010000000
VERSION     DACEPW010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.387"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1193
                     /locus_tag="IW819_002894"
     CDS             <1..>1193
                     /locus_tag="IW819_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1245966.1"
                     /translation="LGWLLALFIGWMSVFYFQELRQFDISKLSLSTIEIVRSIKDLIY
                     LLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILENHWYEVKQTQSEGFFKDLNS
                     SRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLKLEKKLESGLYCELVEKELK
                     DSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAYDSLPHMLIAGGTGGGKTYF
                     LLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQKEEISACVEDFYERMMTRSK
                     AMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTKESAVILNKLKQIVMLGRQS
                     GFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYSMMFGEVDKKFFMKRIKGRG
                     YVDTGG"
BASE COUNT          354 a          195 c          271 g          373 t
ORIGIN      
        1 cttggctggc tactcgctct gttcatcggt tggatgagcg tcttttattt tcaagagctt
       61 agacaatttg atatttctaa gctatcactt tctactatag aaatcgttcg ttctataaaa
      121 gatttaatct atttgctagg aagtctcgtt ctttcaggaa gcacgatgtt gctttatata
      181 cacttttttc aggatcattg gcgcagtttg tggcatcgac aaaagctagc ccggatgatt
      241 ctagaaaatc attggtacga agtaaaacaa actcagagtg aaggattttt caaagacctc
      301 aatagtagtc gaaccaaaga gacaatcagt tacttcccca aaatctatta ccgtatgaag
      361 gatggtttac tttctattcg cgttcaaatt tcactgggaa aatatcaaga tcagctttta
      421 aagctggaaa agaagttaga aagtggtcta tattgtgaac tagtagaaaa ggaactcaaa
      481 gactcctatg tggagtacac tttactgtat gatataattg ccaatcgaat tgggatagac
      541 gaagtagtgg cagaaagtgg ggctttgcga ttgatgaaaa atcaagtatg ggcttatgat
      601 tctttacctc atatgttaat cgctggcggc acaggtggtg ggaagactta ctttttactc
      661 actatcatcg aggctttatt aaaatctgat gccgagcttt ttgttcttga tcccaaaaat
      721 gcggacttag ccgatttagg tacggtaatg cctcatgtct attcccaaaa ggaggaaatt
      781 tctgcttgtg tggaagattt ctatgagcgc atgatgactc gtagcaaggc aatgaaagaa
      841 atgcccaact acaaaacagg agagaattat gcgtttcttg gacttccacc aaacttttta
      901 atctttgatg aatacgtggt ttatatggaa atgttaacga cgaaggaaag tgcggtgatt
      961 ttgaataagc tcaaacaaat cgtgatgtta ggtcgtcaat ctggtttctt tctgattcta
     1021 gcttgtcaaa gaccggatgc gaaatattta ggagacggga ttcgcgatca atttaatttc
     1081 tgggtggctt tgggtcgtat gagtgaactg ggatattcca tgatgtttgg cgaggttgat
     1141 aaaaaattct ttatgaagcg tatcaaaggt cgaggctatg tagatacggg agg
//
LOCUS       DACEPW010000375         1187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.388, whole genome shotgun sequence.
ACCESSION   DACEPW010000375 DACEPW010000000
VERSION     DACEPW010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.388"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            126..800
                     /locus_tag="IW819_002895"
     CDS             126..800
                     /locus_tag="IW819_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1245967.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            1033..>1187
                     /locus_tag="IW819_002896"
     CDS             1033..>1187
                     /locus_tag="IW819_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ1245968.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMH"
BASE COUNT          419 a          152 c          247 g          369 t
ORIGIN      
        1 tatgatttct tagattcaat tagtaaatta gaaaagatga atgattgagt gatcttcaat
       61 actgacttta tacttcaagt aacgtataat acttttatat agtaggaaga aaggggataa
      121 ttaatatgga aagtaatgat tcttatttag agtatgcaaa agcaaataag gataagttta
      181 tttcttatat tatcaaaggg aaatcccccg aagttggacg agacgcagtt tttatggcag
      241 ggagtcctgg ggctggaaaa tcagaggttg ccattggatt agctgagaat tatgataacc
      301 atgtgattat tgatgcggat tactttcgca cccagtttcc tgattataat ggaaaaaact
      361 ctagtctctt tcaaaaagca tcttcttggt tggtagagca ggcatttaga tatgtcttag
      421 atcatggcta ttcttttatt ctggatgcaa cttttgcaat attaagtgct gaaaaaaata
      481 tctcgcgggc gttgaaaaaa cattttcgag tgacgatttt ctatgtttat caagacccta
      541 aaatcgcttg gaaatttaca caagaacgtg aaattgcaga gggacgtcat gtgccgaaaa
      601 aggcatttat taacgctttt ttcaaatcaa gagagaacat tgcaaaagta aagagtaaac
      661 atccagatgt attgcttcat atcataataa aagattatac gaataatatt tccgaagttc
      721 attatgctac ggataatcta gaattatttt taccactagg gtatagtagg aaagatttgg
      781 aggttcaatt acatgactga gaaagaacaa gtaacgaaaa ttgtaaagaa atacaataag
      841 agcattgcgg atttatcgga aaatgctact gcaaaagagt tcaaaacggt gattaaatat
      901 gtagccgatc aggccaatga aaaacaacgg aaattagttg gattaaataa aaaatagtag
      961 gacaaattaa taatgtatga gccaagagca aaatgttctt ggcttttttc tttgcaatga
     1021 atgaggtaat gtatgagatt tttagattta ttcgccggta ttggtggctt tcgtttaggt
     1081 atggaacagg cgagccatca ttgtatcggt ttttgtgaaa ttgataaatt tgctagacgt
     1141 agttataaag caattcatga tacaagtaag gaggtggaaa tgcatga
//
LOCUS       DACEPW010000376         1180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.389, whole genome shotgun sequence.
ACCESSION   DACEPW010000376 DACEPW010000000
VERSION     DACEPW010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.389"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1113)
                     /locus_tag="IW819_002897"
     CDS             complement(<1..1113)
                     /locus_tag="IW819_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAQ1245969.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVL"
BASE COUNT          316 a          248 c          233 g          383 t
ORIGIN      
        1 aaggacagct ccaattaatc gattggcaga cgttcggttg ggaaaaatcc gaataatctt
       61 ttctcttctg cggacttcct ggttcagtcg ttcaagaagg ttggtgcttt ttagccgatt
      121 atgactattt ccgataaccg tgtattgaaa ggcatcttcg aagccattat ccaatatttc
      181 gcaggctttt gtatatttag gctggtcgat atattcaccg actaaggcat tcttagctgt
      241 tcgagcgagt tcaatatccg taaacttaaa gatcgctttt actgcttctc taaacggttt
      301 tgaattcttt tttggaatgg aactgaagat gtttcttaaa aaatggacct ggcatctctg
      361 ccaacttgcg ttggtaaatg acttacgaat cgcagacact aggcctttat gggcatcaga
      421 aatgatgagt tctgtcccct ttaggccgcg ttcttttaag tattcaaaga agatggacca
      481 tgtgtcatca ctttcttcat tttgaatcat gaagccaatg atttcacggt cgccaccttc
      541 tgttatcccg atcgcaatat ggcagctttt agaaagcact cgatggtcct cacggacttt
      601 tatgtagaga acatcagtca tcagataagg ataattcgta cctgagagtg aacggttctg
      661 ccattcgttg accatcgggt ctaactgctc agtcaggcta gaaacaaaag atttcgatac
      721 agattttcca catagctctt caacaatctt tgaaacttta cgtgtcgaaa cgcccgaaac
      781 atacatctca agcattgaag cgagcagtgc cttttcattt cgctgataac gctcaaacac
      841 cgtcggtgaa aattcaccat cacgtgttct aggcactttt aattcgagtg tacccacacg
      901 agtcgtaaag tctcgctcat aatagccatt tctttgactc tgacgacttt ctgaacgttc
      961 atagtcatcg gcttgaatat attctgttcg ttgattttcc atcaattggt tgaaaacagt
     1021 ggttaaaata tttttagaaa catcatcttt taccgaatgt tcaataatac tttgaacctc
     1081 ttcgttgttc agtgtaaaat gtacttgggt catgtaacgt cctcctgggt atgtttttgt
     1141 ggttaaaaac attgtaccgt aaaagggctg ttacatggcc
//
LOCUS       DACEPW010000377         1173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.390, whole genome shotgun sequence.
ACCESSION   DACEPW010000377 DACEPW010000000
VERSION     DACEPW010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.390"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..873
                     /locus_tag="IW819_002898"
     CDS             <1..873
                     /locus_tag="IW819_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1245970.1"
                     /translation="YKLNFRKVNDLDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGL
                     NFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGEAKKLLKNINLANINYSDELPLS
                     PLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKN
                     NFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQFFHILG
                     SVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLI
                     SYPIIEKQQIKYIN"
BASE COUNT          402 a          158 c          200 g          413 t
ORIGIN      
        1 tataagttga attttcgaaa ggtaaatgat ttggatactc attatgcaat gattggtatt
       61 gccgctggta tagctgacta ccctctttat tacgatgcga caaatgaaaa aggattgagt
      121 atggctgggc taaatttttc tgggtatgct gattataaag aaatacaaga agggaaagac
      181 aatgtatctc cttttgaatt tattccttgg attttaggac aatgctcaac agtaggagaa
      241 gctaaaaaat tgttaaaaaa tatcaattta gcaaatataa attatagtga tgaacttcct
      301 ttatcccctt tacattggct attagctgat aaagaaaaat caattgtcat tgaaagtatg
      361 aaagatggac ttcatatata tgataaccct gtgggcgttc ttaccaataa tccttcattt
      421 gactatcaat tatttaattt aaacaattat cgtgtcttat cgagtgaaac tcctaaaaat
      481 aatttttcaa atcaaataag tttgaatgcc tatagccgcg gtatgggagg gataggcttg
      541 cctggagatt tatcctcagt atctcgtttt gttaaagcga cttttacgaa gctgaattct
      601 gtatctggag attcagagtc agaaagtatt agtcaatttt tccatatctt aggttcagta
      661 gaacaacaaa aaggtttgtg tgatgttggt gatggaaaat atgaatatac aatttattct
      721 tcttgttgca atgttgacaa aggaatctat tattatcgaa catatgaaga cagtcaaatt
      781 actgcaattg atatgaataa agaagactta gatagtcata agttaattag ttatccaatt
      841 atagaaaaac aacaaattaa atatataaat tagttaatgt gttgtgattg attatttaat
      901 atagatataa tacaaaaagg caaagatatt ttcaaaacat tttttcttga tgtgatatcc
      961 ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa aatttattta cctgttttct
     1021 ggatcaacga tagtgtatat tatgctatcc ttgtttggat tttaggacgt ttaaagaggc
     1081 acttttttac ataacttaat ccatagacct tttaagcatg ctgtgagata ataataaaga
     1141 agagtttaaa gcgcacccca aaccacttcc cca
//
LOCUS       DACEPW010000378         1164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.391, whole genome shotgun sequence.
ACCESSION   DACEPW010000378 DACEPW010000000
VERSION     DACEPW010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.391"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            34..906
                     /locus_tag="IW819_002899"
     CDS             34..906
                     /locus_tag="IW819_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1245971.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          335 a          223 c          280 g          326 t
ORIGIN      
        1 tgatttcatt ctaaaataaa gggagaaatg tctatgctat acggtgcaat tgaagcaggc
       61 ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga cgatcaagga aagagtgagc
      121 tttccgacaa cgacacctga ggaaacaatg cctttagtca ttgacttctt caaacaatac
      181 caagcagatt tagcagggat tgggattggc tcatttgggc ctatcgatat ccatcgcgat
      241 tccgccactt atgggtacat tacctcgacc ccgaaattgg catggcaaaa ttttgatttc
      301 atcggtacca tgaaaaaaga atttccgatc ccgatttctt ggacaacaga tgtcaatgcg
      361 gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat tgagcagtgt cgtttactat
      421 acgatcggta cgggtgttgg cggcggtgcc ttacaagaag ggcgctttat cgaagggttt
      481 agtcatccag aaatggggca tatgctcgtc gtccctcatc caaaagatga ctttgctggc
      541 agctgtccat ttcatggcaa ctgtttagaa gggatggccg ctggacccgc catcgaaaac
      601 cgcttaggga aaaaaggaca agaggttgca gaagatgatc cttattggga aatcgaagcc
      661 tcctatattg ctcaatgtgc ctacaataca accttgatgt tttctccaga tgtgatcatt
      721 tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa acgtccacga cgcatttgca
      781 aaattggtca atgggtatgt cgaaacacct gagttaagca aatatatcgt gaccccagca
      841 ttagaagaca acgctggaac gctcggttgt ttggcattgg ctcgagaagc ggtcttacat
      901 tcataaacga aaaaaggctc ttcgacattt agtgttggta gagaaagata caactgaaaa
      961 tttagtcata accagttagg cagagtgctt aactggtttt tgttttactt ggaagatcaa
     1021 gccttgaatg agataaattc ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac
     1081 ttgtattgtg caatagtttt ttacaaaaaa aagggggtgc ggatattttt ttggaccttt
     1141 ttaacaaata tatctttgct tctt
//
LOCUS       DACEPW010000379         1158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.392, whole genome shotgun sequence.
ACCESSION   DACEPW010000379 DACEPW010000000
VERSION     DACEPW010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.392"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..400
                     /locus_tag="IW819_002900"
     CDS             2..400
                     /locus_tag="IW819_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1245972.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IW819_002901"
     CDS             384..1079
                     /locus_tag="IW819_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1245973.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          444 a          170 c          244 g          300 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaattt
//
LOCUS       DACEPW010000380         1156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.393, whole genome shotgun sequence.
ACCESSION   DACEPW010000380 DACEPW010000000
VERSION     DACEPW010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.393"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..831
                     /gene="fss3"
                     /locus_tag="IW819_002902"
     CDS             <1..831
                     /gene="fss3"
                     /locus_tag="IW819_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1245974.1"
                     /translation="ATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQV
                     LDKTIVLFNYVYENKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGS
                     QTFTHGDVVDMFDDVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDK
                     EFTKTVLAEKVDTSKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTT
                     PSAPEQPEIPTVPSDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN
                     "
     gene            945..>1156
                     /locus_tag="IW819_002903"
     CDS             945..>1156
                     /locus_tag="IW819_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1245975.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQP"
BASE COUNT          427 a          211 c          250 g          268 t
ORIGIN      
        1 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
       61 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
      121 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
      181 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
      241 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
      301 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
      361 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
      421 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
      481 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
      541 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
      601 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
      661 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
      721 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
      781 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
      841 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
      901 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
      961 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     1021 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     1081 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     1141 ttgatcaaga tcaacc
//
LOCUS       DACEPW010000381         1141 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.394, whole genome shotgun sequence.
ACCESSION   DACEPW010000381 DACEPW010000000
VERSION     DACEPW010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.394"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..861
                     /locus_tag="IW819_002904"
     CDS             <1..861
                     /locus_tag="IW819_002904"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1245976.1"
                     /translation="NILDDGKHIVPEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDS
                     EAGTVIGAYNLNWTEVQFVKKLIEAGTGIKFAIDNDANVAALGERWKGAGENDPDVVF
                     VTLGTGVGGGIVAGGNLIHGVSGAGGEIGHITVDPEGFECTCGKRGCLETVSSATGVV
                     RLGRFLAEEYAGDSKLKAMLDNGEEVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACG
                     NLGNTLNPSSIVLGGGVSAAGEFLRSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVI
                     GAASLALKFA"
     gene            879..>1141
                     /locus_tag="IW819_002905"
     CDS             879..>1141
                     /locus_tag="IW819_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1245977.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPV"
BASE COUNT          354 a          180 c          293 g          314 t
ORIGIN      
        1 aatattttgg atgatggtaa gcatatcgtt cctgaaatca ttgaatcaat caatcatcgt
       61 ttgaatttat ataatatgaa agccgaagac tttatcggaa tcggcatggg taccccagga
      121 agcgtggaca gcgaagctgg aacggttatt ggtgcgtata acttaaactg gacagaagtt
      181 caatttgtga aaaaactgat cgaggcagga acaggaatca aattcgctat cgataatgac
      241 gcgaatgttg ctgctttagg tgaacgatgg aaaggtgctg gagaaaacga tccagacgtt
      301 gtattcgtta ctctaggaac aggtgttggc ggaggtatcg ttgctggcgg aaatttgatt
      361 cacggcgtga gcggagctgg aggagaaatc ggacatatca ctgtggatcc tgaagggttc
      421 gaatgtactt gcggtaaacg cggatgttta gaaactgtat ctagcgctac aggtgtggta
      481 cgcctaggtc gtttcttggc agaagaatac gcaggggatt caaaactgaa agcaatgcta
      541 gacaacggag aagaagtaac aagtaaagat atctttgaaa tggctcaaga agacgatccg
      601 tttgcattga tggttgttga tcgcgtatgc ttctacttag gtttagcttg cggaaacttg
      661 gggaatacat taaatccgtc aagtatcgtg ttaggtggag gagtatcggc agcaggagaa
      721 tttttacgta gccgtgtaga gaaatatttt aaagaattta ctttcccgca agtaagagaa
      781 tcaacaaaaa tcaaattagc agagcttggt aatgaagctg gtgtcattgg tgctgcctca
      841 ttagccttga aatttgctta aaaaaacgga ggcattacat ggttttatgg gtaattaata
      901 tcattttgct attgattgtg cttgcaatca tcctttggga agtctatcta cgagtaatgg
      961 ccaaacgttc agcaacgact ttgacagaag aagaatttcg agaaggaatg agaaaagcac
     1021 aagtaatcga tgttcgcgaa aaagatgtgt ttgatgctgg gcatatttta ggtgcaagaa
     1081 acatccctta cactgttttg aaagattcat taggatctat tcgaaaagac cagcctgtct
     1141 a
//
LOCUS       DACEPW010000382         1119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.395, whole genome shotgun sequence.
ACCESSION   DACEPW010000382 DACEPW010000000
VERSION     DACEPW010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.395"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(25..450)
                     /locus_tag="IW819_002906"
                     /pseudo
     CDS             complement(25..450)
                     /locus_tag="IW819_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            523..>1119
                     /locus_tag="IW819_002907"
     CDS             523..>1119
                     /locus_tag="IW819_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1245978.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGI"
BASE COUNT          346 a          211 c          224 g          338 t
ORIGIN      
        1 gttcttccct actgtagatt gtttctaatt ctccatcggt tgactcagca tttgttcgag
       61 ctcgccttcg gcctgttgga aacctttatg actgcgacct aggaattttt ggttgtaagt
      121 atcaaaagac gagactaaga aacgttccat cgactcttcg ttttggaact gttccttgcg
      181 gtggctgtat ttcttgattt gcttattaaa ggattcaatc aagttcgtag aataaatact
      241 cttgcgtatg gccaagggga aatcatagaa agtgagcaag tgatcgttcg aaagaatcga
      301 ttcaaccact tttggatagc tggttttcca ttttttcgca aaagcaccac gtgcttctaa
      361 cgccacctct ttagatgagg cttgatacac cattttaaaa tcatcacaga cttccttacg
      421 attattgaca cgcactttgt gactgatatg gttctgttgc aaagttttcc aaaaaatcta
      481 ttttagtgta aaattgagaa aaaagacaga gaggacagag taatgaatca ttttaaaggc
      541 aaacaattca aaaaagacgt cattattgtc gctgttggtt actacctgcg ttacaatcta
      601 agctatcgtg aagttcagga attgttatat gatcgtggaa taaatgtttg tcatactacg
      661 atttatcgtt gggtgcaaga gtacagcaaa gtcctctatt atctttggaa gaagaaaaat
      721 agacaatcct tctattcatg gaaaatggac gaaacctata tcaaaattaa gggacgttgg
      781 cattatcttt atcgtgcaat tgatgcggac ggcttaacct tagatatctg gttacgaaag
      841 aaacgggata cgcaagcagc ctatgctttc ttaaaacgac tccataaaca gtttggtgag
      901 ccgaaagcaa ttgtgaccga taaagcacct tctcttggct ccgcctttag aaagttacag
      961 agtgtgggtt tatatactaa gacagagcac cgaactgtga agtatcttaa caatttaata
     1021 gaacaagacc atcgacctat taaacgacga aataaatttt atcaaagtct ccgtacagcc
     1081 tcttccacga ttaagggcat ggagaccatt cgaggaata
//
LOCUS       DACEPW010000383         1109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.396, whole genome shotgun sequence.
ACCESSION   DACEPW010000383 DACEPW010000000
VERSION     DACEPW010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.396"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..474
                     /locus_tag="IW819_002908"
     CDS             <1..474
                     /locus_tag="IW819_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1245979.1"
                     /translation="ISDLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNK
                     LRKTILEYVQQEFPDQLLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDI
                     GPHSMRKTFGYFLYKQGTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            538..>1109
                     /locus_tag="IW819_002909"
     CDS             538..>1109
                     /locus_tag="IW819_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245980.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          393 a          170 c          220 g          326 t
ORIGIN      
        1 atttctgatt tgcgtcaatt gaagctggcc gacgtgctgg aaatttcacg cggtcgcgtg
       61 gtggtcaaag atcgattagc gatgaaggaa caaaaaacag ctaaacataa ttcggtctat
      121 atttccaaca aattgcgaaa aacgattttg gagtacgttc aacaggaatt tcctgatcaa
      181 ctccttcagc aagattttga cggttatttg ttccctagtc gaaaagggac gaatgaaccg
      241 ctcaaccgcc aaagtctgtg gcgaatcatt catgatgctg gcacggcaat cggattgaaa
      301 gacattggcc cacattccat gcgaaaaacg ttcggttact ttctctataa acaggggacc
      361 aaaacagaaa tcattcagtc cttgttgaac cattcttctc agcgcgaaac attgcggtat
      421 attggtatta cacaagaaga caaagataca gctgtaaaaa gcttagattt ataatataaa
      481 agttaagttt aaacatggat agttttaaat tagtagattg agtgttggga ggaaagaatg
      541 agtacattat atattatagg aaacggtttt gatttaagtc atggtttaaa aactagatac
      601 actgattttt caaaatgggt tgaaaaaagt catagtgaaa tattcgataa aatagacaca
      661 atgataaatg aaaataatga ggtgagaagt acagctgatg ataagatagg ttggtttaat
      721 tttgaaagta gtttgcatga gttaattcaa agtccagtct ttgaagatta tgtagaagag
      781 tctaaggatt atctcatagg ttatggacat gaagattggt cagattctgc ccatcatgat
      841 tttcaaaata tggtttatga ttatgttgtt tctctagaag aattacaaca acttttcaag
      901 gaatggatac gcaaagaaat acagccgaca atagaacata aaattccaaa ataccaattt
      961 tcgtccgatg tactattctt tacattcaat tatacagatg ttttggaaat tctatacaag
     1021 caaccgaaag aaaacatata tcatattcat ggcagttatg acgatataat aatcggccac
     1081 tccgagaatt ttgacaactt tataggaga
//
LOCUS       DACEPW010000384         1106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.397, whole genome shotgun sequence.
ACCESSION   DACEPW010000384 DACEPW010000000
VERSION     DACEPW010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.397"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            391..777
                     /locus_tag="IW819_002910"
     CDS             391..777
                     /locus_tag="IW819_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1245981.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            871..1062
                     /locus_tag="IW819_002911"
     CDS             871..1062
                     /locus_tag="IW819_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1245982.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          415 a          162 c          174 g          355 t
ORIGIN      
        1 aaattttcgt tcaaatacgt gtctattcga actcttttct agttgttaat tatgaagggg
       61 gaagaaataa aaaatacaat ctatacacga ataagaatat tatccaaaag gaggttttag
      121 aaaagattta ttatacgtaa aaagctcaag acattttctt agccataagg acatgtttga
      181 tatttaagaa gatatatgaa ttttcttttc taagtataaa gataactatc cattatcaac
      241 taaaaaaaca tttttgaaaa taaacatcag cagtcctatc aatgcatgtg agcttataga
      301 agttattcgc agcgcagcat aattgcatta gcttcttttc gtgttttttg cctcttttcc
      361 ctttaaatct taatggagga ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc
      421 cagttgattc gattctttga aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa
      481 caagaagaaa ttcaaaaaca tgaacaactt gatcaactcc caccatattt gtttactaaa
      541 gatactataa aatttaaaca accgattaat gttttaaatg tgactctcct tgttgaagat
      601 ataaaattag caaaaatcct tcctttgttg aaagaacctg aacaaatttt tcttatcgaa
      661 aaatttatct ttgataaaac tgataaagaa attggaaagg ttctggggat tacaagacag
      721 ggtgcgacaa atttaaaaca tcgactttac aaaaaaattc aaaaaatgct ggtttaatat
      781 ctgcataata actaattttg tatgcttctt tgctcgttct cctccttccc aaagaaatag
      841 attataagtc catgggaaga aggtagattt atgaattctt taaaagaaga tttcatttta
      901 gcaaaggctg gaaatgaaga agctgttgag gcaattctca aaagatttag ctctttgatt
      961 cataagcaat cctggagaac tggaaaatat gatcaagatt gttatcaaga atgtatgctt
     1021 gcaatatatc tagctatctc gaaattcgaa attaaagaat aactcaacta agggagtctg
     1081 tgacataagt aaaaagatag tttcaa
//
LOCUS       DACEPW010000385         1105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.398, whole genome shotgun sequence.
ACCESSION   DACEPW010000385 DACEPW010000000
VERSION     DACEPW010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.398"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(832..>1105)
                     /locus_tag="IW819_002912"
     CDS             complement(832..>1105)
                     /locus_tag="IW819_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1245983.1"
                     /translation="GKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLP
                     EELDIKGQVIISQLKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF"
BASE COUNT          356 a          159 c          169 g          421 t
ORIGIN      
        1 atagctttta ttatagaagg tattgtaaat aataatattg acaaaaagtg actcaaaata
       61 aaaaaaacct tacattatac tggaatgaac tgtattgata gtgtacctaa aagctctact
      121 gacataagat cgtgtaatcg gtaaagtaat tttattaatt atacgggatg taatcaatta
      181 gagaataaac taatcataaa aggtactttt acattcataa ttgtcgcttt gagattctac
      241 tttggttcac tatctgaccc aattgcacat tttcgatcat tacaagtatt ggtgtcgtta
      301 gaacaactaa ataaaaacta gcggtagatt tttcagaatc tatgctaatt tctgtatgga
      361 tgttccagtg atggcaaatt tctcttttca ttatttttct tgtcgcatga tatgcctttt
      421 tgtgttattc agttaaacta tagaaattct ttcctagaca gatcttgatc ccaaatttta
      481 cgtttttgag catcgatata gtttaaaatg acattactca tatctaaaga gtttagattt
      541 tttaggacta aaactgtcag ttgttctttt tcttctttct gttaaagtat tgtcgcgact
      601 tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg
      661 acttgaggaa atcacctcaa tcattcgttt aaaatgctat atattcttta gtttaagata
      721 cggaaattaa tgccatcgac cacactagta gaatgaacga catttacttg gccttttttt
      781 aatttcgtta tgtttagcat ataaaaatat tatagagttt acttacaatg cttaaaacat
      841 atactgtata atctgatcaa ttttagccat gtcttgcaat ggtaagtttt ctattttttt
      901 gagttttcgt tccttaaaat caagagattt taattgtgaa ataattactt gtccttttat
      961 atctagctct tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg aagtaattgg
     1021 acatatgacc gaaaacattg ttttacggtt aaattcataa cgagagacga ctagtcctgg
     1081 tcgtcttttt tgaatttctt ttcct
//
LOCUS       DACEPW010000386         1093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.399, whole genome shotgun sequence.
ACCESSION   DACEPW010000386 DACEPW010000000
VERSION     DACEPW010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.399"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(206..436)
                     /locus_tag="IW819_002913"
     CDS             complement(206..436)
                     /locus_tag="IW819_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245984.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(686..886)
                     /locus_tag="IW819_002914"
     CDS             complement(686..886)
                     /locus_tag="IW819_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1245985.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          391 a          176 c          158 g          368 t
ORIGIN      
        1 aaaaagtata cacccttctt cattctagtt ttcgcagcag gaattttgaa atagttctta
       61 ttagaatagt agagattgat taataagcta tcttcgattg ctaaaaagaa aacttctgaa
      121 tcccacacct tataaaaatc tttgacaaat ctattcgaag gatcaaattt aaaccataat
      181 tgcgtctcat taaaaagcat aaccattact ccgatctatt tttaaactta gtctctacct
      241 ctaatatata tcgagttttt attttgcctt cagagaatac tgtttctttt tttgcagtta
      301 caggttgttt attttcggaa aaagctaata tagctaaaat tgaaacatcc atctggaatt
      361 tgtctttttc gttgctttgc tcataaaaat ctgcatattc atcactaata ttttttctaa
      421 taaattcttc catcataaag atcacctcga aaagagtata cgaacaaacg ttctttttgt
      481 aaaggtggaa tttattatac taaataaata ggtgaacaag tacttgtgcc aaattatgtg
      541 ccaaaaaaaa tcgaatttaa taaaacacaa acaaaaagaa atcctattat gataacattt
      601 cttataataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      661 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      721 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      781 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      841 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      901 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
      961 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
     1021 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
     1081 ccatataaaa aaa
//
LOCUS       DACEPW010000387         1077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.400, whole genome shotgun sequence.
ACCESSION   DACEPW010000387 DACEPW010000000
VERSION     DACEPW010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.400"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            67..>1077
                     /locus_tag="IW819_002915"
     CDS             67..>1077
                     /locus_tag="IW819_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1245986.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSP"
BASE COUNT          445 a          160 c          181 g          291 t
ORIGIN      
        1 ttgctatttt cgaaaagagt aggtaatttt acataaataa aacaacgtga aagtagagga
       61 tctacaatga ctgattttaa attttttaag gcggatagag tttacaacga attattttat
      121 caatttccaa aagtctttat tgtttctgac gaatacaaaa aaatgaaaga ttcaactaag
      181 attgcctata tgcttttaaa agcaagatta gagatcgcaa tcagcaaacg gcaaatcgat
      241 gaagaaggta atgtttattt tacttatacg acaaatgaac tatgtagagt attaaactgc
      301 caaaaacaaa aagcgatagc aatcaaaaaa gagttggaat cctttggttt attattacaa
      361 aagcagatgg gatttaacaa acagttaggg aaaaataatc ctaatagact atatctagca
      421 gaattaaaag tctcagaaaa tgatatctac ttactcgaaa aatttgatag agagaatagg
      481 gaaaacgttg ataaatcaga gggtatgaaa atcataccca ccctcgacga aaaatcagac
      541 gctgaatccc ttggggctca agagggtatg aaaatcatac cgtgccaaaa cgttgataaa
      601 tcagagggta tgaaaatcat accagaactt aataataata tattagacac taatagacac
      661 aatatagaca ctgaaaaaga ccgcctacaa gatcaattgt tgttagacaa ttttgagaca
      721 attatgacaa acgacagcat tgctacgttt gtccctgaac gagtattaaa tttgataaaa
      781 acattttctt caagttacag tgaagctcaa aaaaccgtcc agactattca taatgcaaag
      841 aaaaaagctg aaatagaaag tggtatttcg atagtttttg aagaactcga tagttattat
      901 gtcaatgcag aacaagaatt atacacgaca ctgttaaaag cctatcaaaa attaaaaacc
      961 gaaaaagtcg aaaatatcca gaacctgatt tttgtctatg taaaaaattg gtttatcgaa
     1021 aaaccaatag ctgctaaagt atcaagtgaa aaacgtttga attatgaaag ctcccca
//
LOCUS       DACEPW010000388         1047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.401, whole genome shotgun sequence.
ACCESSION   DACEPW010000388 DACEPW010000000
VERSION     DACEPW010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.401"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            282..521
                     /locus_tag="IW819_002916"
     CDS             282..521
                     /locus_tag="IW819_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1245987.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          342 a          194 c          153 g          358 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaatt
//
LOCUS       DACEPW010000389         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.403, whole genome shotgun sequence.
ACCESSION   DACEPW010000389 DACEPW010000000
VERSION     DACEPW010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.403"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(22..>1035)
                     /locus_tag="IW819_002917"
     CDS             complement(22..>1035)
                     /locus_tag="IW819_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1245988.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
BASE COUNT          272 a          222 c          162 g          379 t
ORIGIN      
        1 acggttttca tcatgacgat ttcactccct ttcttgctgt ctaccttgtt gaaacaacat
       61 ggcacgtttt tgctttctga tctcttgttg gtagatttca tgcttcaatt gatcattcca
      121 atcctttttt acttcaggta caaattcatg ttcatgattt ttatagcctt tgtagtattg
      181 ttctgcttta taagagaaaa gaatctgagc attaatatcc cctttttctt gataaaaacg
      241 atcaaaatcg tacgtaccat ccacgcgttc acaagctttt aattcttttg cgacttctct
      301 acaaatatct ttcaaattaa tagcttctat tgaatcgtta acagaaagct gctgaccaaa
      361 agacgcattt actttgtgag cggaaaccaa ttggttcgtt aattgcagat tgttttctgt
      421 cttgtgtatg gccataatag cttctggtgt gatcttttct tcttttgcga cttcttcata
      481 aatgactttg tattcttttg gcacttgcca atcattagcg attcctcgtt cacaacgtac
      541 tttctgattc gtaaatggat caactgcgcc cattaattgt tctttttcgt agaaaatatg
      601 tcctgcacgg tcattatcca cacaaatctc gatggattga ggaaaggctt gtttctttct
      661 caattcagat actgcctcac caaaataatg actgatcaca tggtgtttta acccttccat
      721 cgaaactaac cacgtatcgt ttaactgatc acgatttagt gcagcatagc taagtaaatc
      781 aatactactc tcaaaaaatt ttaactgctt agggtcaccg atctttaggt taaagccatg
      841 attcgctgtc gaatttttat ctatatgctt atatgtgcct ctctcaccaa aacgttcata
      901 atcaatgaac gttccttgat aactcgcccc tactgtctca tttgtctgtg gatctttcca
      961 taaaaaggcg accgcatgat ctaaacgatc gtctcgaact aagaaatttt gccgttcatg
     1021 ttttggcttt agttc
//
LOCUS       DACEPW010000390         1033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.404, whole genome shotgun sequence.
ACCESSION   DACEPW010000390 DACEPW010000000
VERSION     DACEPW010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.404"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..267)
                     /locus_tag="IW819_002918"
     CDS             complement(<1..267)
                     /locus_tag="IW819_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1245989.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNN"
     gene            573..>1033
                     /locus_tag="IW819_002919"
     CDS             573..>1033
                     /locus_tag="IW819_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1245990.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQHGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKINNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENN"
BASE COUNT          363 a          169 c          142 g          359 t
ORIGIN      
        1 attgttgtag tcatgataag ctttttccac tacttcttca tcaaatgttt cacacacaac
       61 tactcctgtt tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
      121 tcgataaaga tttcctccac caaaatgaag ccatactgga tgttgctggc cttcttgctt
      181 aacttctttg acatcaaaac acggcagtag aatctgcatt tcattgaatt cttcgataga
      241 attttgccaa tttttaatcg aaaccataat taacctcctt aaactaatat gttagttttg
      301 atacatttta tattttaaaa cccgttgtaa ccgttgtcaa tataaaaatg gaataaactt
      361 gctttttttc taccatatca gaattattgt attttttatt cagaaaaata aaatactcct
      421 tctttttgga ctgattttat cgaaacttat ttttttcttt ttttcatgta ttcttttatt
      481 tttataacta gtgtattact tcaaaatttg atattatatg gcatgaacag aattaaaata
      541 tcagctaaaa taaaagttaa ggagcattga gtatgacaat taaaactagt ctccaaaatc
      601 aagcttacca atctattcgc cgacaaatta tctatgctga cttagaacct ggaaaaaaag
      661 tatcagaaaa aaaattagaa gaaacacttt ctattggtcg tacaccaata cgtgaagcac
      721 tgattcaact ccgacaacat ggtcttgttg atacaattcc tcaaagcgga aattatatct
      781 cattaattga tttagattcg gccaaaaatg ctcgatttgt cagagaacat ttggaacgac
      841 aaattatgtt agagtgttgt gccaaaatta ataaccaaaa aaaacggatt ttagaaacta
      901 taatttcaga acaagaaaaa gctgttagtc aaaaaaatgc tcgtagtttt tttcataatg
      961 ataatttatt ccataaagct tgttttgaaa ttgcagagcg agaagaagta tggcaatggt
     1021 tagaaaataa caa
//
LOCUS       DACEPW010000391         1009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.405, whole genome shotgun sequence.
ACCESSION   DACEPW010000391 DACEPW010000000
VERSION     DACEPW010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.405"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            81..>1009
                     /locus_tag="IW819_002920"
     CDS             81..>1009
                     /locus_tag="IW819_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ1245991.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKA"
BASE COUNT          378 a          184 c          180 g          267 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa tccgtctcta
      481 agtccttcgt ttctagctta acagaacagc tagaacctat ggttaacgag tggcagaatc
      541 gtttattatc agaaaaaaat tatccttact taatgaccga tgtactctat ataaaagtac
      601 gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc gattggaata accaaagatg
      661 gcgaccgtga aattatcggc ttcatgattc aaagtggcga aagcgaagag acctggacaa
      721 cattttttga atacctaaaa gaacgcggtt tacaaggtac ggaactcgtt atttctgatg
      781 cgcacaaagg attagtctct gccattagaa aatccttcac caacgtaagt tggcaaagat
      841 gccaagttca cttcctaaga aatatcttta ccaccattcc taaaaaaaat tcaaaatctt
      901 tcagagaagc tgttaaagga atttttaagt tcacagatat taacttagcg cgtgaggcta
      961 aaaatcgatt gattcatgat tatatcgatc aaccaaaata ttcaaaagc
//
LOCUS       DACEPW010000392          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.407, whole genome shotgun sequence.
ACCESSION   DACEPW010000392 DACEPW010000000
VERSION     DACEPW010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.407"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>994)
                     /locus_tag="IW819_002921"
     rRNA            complement(<1..>994)
                     /locus_tag="IW819_002921"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACEPW010000393          975 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.409, whole genome shotgun sequence.
ACCESSION   DACEPW010000393 DACEPW010000000
VERSION     DACEPW010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.409"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..330
                     /locus_tag="IW819_002922"
     CDS             <1..330
                     /locus_tag="IW819_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1245992.1"
                     /translation="DFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLTNIENKGQQP
                     SFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVTIGKVIQAIY
                     GEKCKFN"
     gene            complement(844..>975)
                     /locus_tag="IW819_002923"
     CDS             complement(844..>975)
                     /locus_tag="IW819_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1245993.1"
                     /translation="MREDTELKNFPLFCPKCRQEILIEITKFRITVITEPDAKTQSR"
BASE COUNT          324 a          137 c          176 g          338 t
ORIGIN      
        1 gatttcaaac cattgggtct tgcgattaaa gaagcccgtc ttagtaaagg attgactcgt
       61 gagcaggtag gatcaatgat ccagattgcg ccacgctatt tgacgaacat tgaaaataaa
      121 ggacaacagc caagttttca tgttgtatat gatttagtga ctttgctaaa tatttctttg
      181 gatggattta ttttagggga agaaaacagt cataaaagct ccaaacgtct tcaagtagaa
      241 gcacagttgg attgtctgga tgaagatgaa ttagtgacga ttgggaaagt gattcaagca
      301 atttacggag aaaaatgtaa atttaactag ttcaataata cacaatggat taattaatac
      361 tatcaggaaa gattgattac aaagatgtaa tgaatttttt ctgatttttg ttaatcataa
      421 atagaaaatg aatttatctt tgttacactt aggaactaga gcgaatgata aatagttctt
      481 tggttactct tatcgctgca aagtacagtt cattttttgt tgttgggaaa attttctgga
      541 tttattgcat atcacttcat ccgactaaaa atttttgatt cccatagcga agttcgtttt
      601 tgttccttag gaaaaacttg actgatatac tagttttgaa atgacgagta actctgtact
      661 tcatcaataa tcaagtattg aattttaatt gtatactttt tatgcttgag ggatgtctac
      721 tgtaatacag ctactgcaaa aaaaatcagt acacttattt tcttataaag gatatcaaga
      781 actaagctca atggtatgca tagaaaagaa aaagccgata aaatgagatt ttgatatctc
      841 gaattatcgg ctctgcgtct ttgcgtctgg ctctgtaatt acagttattc taaacttcgt
      901 tatttcaatt aaaatttctt gtctgcattt aggacaaaat agagggaaat tttttaattc
      961 agtatcttcc ctcat
//
LOCUS       DACEPW010000394          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.410, whole genome shotgun sequence.
ACCESSION   DACEPW010000394 DACEPW010000000
VERSION     DACEPW010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.410"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>943
                     /locus_tag="IW819_002924"
     rRNA            2..>943
                     /locus_tag="IW819_002924"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACEPW010000395          935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.411, whole genome shotgun sequence.
ACCESSION   DACEPW010000395 DACEPW010000000
VERSION     DACEPW010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.411"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..392)
                     /locus_tag="IW819_002925"
     CDS             complement(<1..392)
                     /locus_tag="IW819_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1245994.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKV"
     gene            complement(409..813)
                     /locus_tag="IW819_002926"
     CDS             complement(409..813)
                     /locus_tag="IW819_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1245995.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          283 a          160 c          151 g          341 t
ORIGIN      
        1 actttgatga atttactatc aatcaaagca actgtaccat tttgattatt cgttgtataa
       61 ctctgaacag gatttttctt acttttgaaa cgtggaaatc caacggattt atcccgaaaa
      121 aaattcttgt atgctttatc taaattaagt tgggcattgg ctaaagcaag gctatcaatt
      181 tctttcaaaa acggaaattc tttcttgtat ttagctggtg tcggaaaagt cattttttta
      241 gaagaatcat ttttaacttc ttcatacgcc ttttttcggt catcaagcat tagattgtag
      301 accttacgga cacaaccaaa agatttggca aagaagattt cttgttcctc tgttggatag
      361 attctgaatt tgtatgcttt tagttgttcc ataaagttca cctactttct atttatgatt
      421 gtttccttga ttttggatat acttcttaat cacatcaatt ggtgcgccac cagttgtcag
      481 aagacaaaaa cttttagacc agaacatttc tttccaaaga aattgtttga ctcttggaaa
      541 gtcacgtttt atcaatcttg agctggcact tttataggca ttgatgaatt ttgtcaattc
      601 tgttttaggt tgagctttga acataatatg aacgtggtct ttatcatgat tccactccac
      661 taaagtaatg tgatacgatt ctgaaattct ttcaaaagta gttttggcat aatcagatat
      721 ttcatcatca atcacttgtc tacgatattt cgttactaaa acaaggtggt aataaagaag
      781 aaatactgaa tgattatttg tatctaattc catgttatat caactcattt cttgtataga
      841 ctgattgtaa catgggacaa aataaaaagg caatacgcct tagttgtcgg tcttcgagtt
      901 accaacggta accctcatac cgaacggcat tcatc
//
LOCUS       DACEPW010000396          928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.412, whole genome shotgun sequence.
ACCESSION   DACEPW010000396 DACEPW010000000
VERSION     DACEPW010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.412"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(57..725)
                     /locus_tag="IW819_002927"
     CDS             complement(57..725)
                     /locus_tag="IW819_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1245996.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          351 a          158 c          152 g          267 t
ORIGIN      
        1 ttttagttta cataatgata tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag
       61 tgctctaact gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc
      121 aattggggca aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga
      181 atagaacagc taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca
      241 actaaaatac caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt
      301 tcaatggctt gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga
      361 ccgcaaccaa tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca
      421 gcaaaatgta tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg
      481 acgatcgtcc ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt
      541 aaattaaaaa gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa
      601 gcaataccag taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct
      661 aatccgtaga ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa atttaatttt
      721 ttcatttcta gcctcttttc ttttgaaaat tttcagcaac ttctttatta tactgactta
      781 tactcttact tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat
      841 caaataagga gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc
      901 aaaaaaacag agtatcaagg agtttttt
//
LOCUS       DACEPW010000397          926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.413, whole genome shotgun sequence.
ACCESSION   DACEPW010000397 DACEPW010000000
VERSION     DACEPW010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.413"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..434)
                     /locus_tag="IW819_002928"
     CDS             complement(<1..434)
                     /locus_tag="IW819_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1245997.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASV"
BASE COUNT          258 a          198 c          139 g          331 t
ORIGIN      
        1 acactggcaa cttcaaaaca aatagggaca tcatctgcga aacgaatacg ttccattcgc
       61 aagatcgtgt catctttact caagtttagt ttttccattt cacttgagct tggcgaagtc
      121 aagaaataag agattgtccg actagacggt actcgatttt gtgattccat aatttctgta
      181 aaacttgttg caccagacat tttttcttgt actttttctc gcgcaacata cgtacccgaa
      241 ccgatctttc tttctaagat tccttcatct gccaagtttt gaattgcttg tctcaacgtc
      301 atacggctga cattgaattt cactgctaat tctctttctg aaggaagacg gtctccgact
      361 ttccatatac cctgttcgat ttcatttttt aattggtcat gaatctgtat atatacaggt
      421 aattgattcg acataacgta cacccgtcct ttcgtttctt ctattataac tggtatatac
      481 ctgattcaca actgaaaaag ataaaggttt ctatccatca acagaggatt tagacacaca
      541 tcgttctccc tactcctatt gtcagaaaca attttttaga tatgttcaac ctagaaaata
      601 acaaaagacc ctaaaatcga tgaaaatgcc gacttatgga tcttttgtta ttattagcgg
      661 tttttagcca tccatttttt tgtatgattc gttgggcgtt ttgattttac ctggaccgct
      721 tttccgcctt ttttcggaat attcacttca atattgtatt tttctgattt gaccaaatca
      781 tataactttt cagctgcttc gtctactttt tctttccatt tcatgtggat ccctccaatt
      841 cttgtagtta acttaactct atcttaattc agcagaatct tcaaggaaca aggtagcaaa
      901 gcaaaaaaaa gagctctcga actaac
//
LOCUS       DACEPW010000398          923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.414, whole genome shotgun sequence.
ACCESSION   DACEPW010000398 DACEPW010000000
VERSION     DACEPW010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.414"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            248..781
                     /locus_tag="IW819_002929"
     CDS             248..781
                     /locus_tag="IW819_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1245998.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          300 a          174 c          164 g          285 t
ORIGIN      
        1 gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt ttttgaaaat ggttcctcac
       61 aactttgcat aaatttgact taataccgag aagtcatgat ccccatctaa ctccaaatca
      121 caaggaatga gttcaaaaac tagcttttct caacaaatat tacataaaaa attctatgtt
      181 ttttgttaaa tttatttgct tttttcaaaa gtatgttaca gtaatttccc aaggaggttt
      241 tacagaaatg aattttgttg ccatggattt tgaaactgcc aatcatcagc cttatagtgc
      301 ttgttctctt gcgctagtca tggtaaaaaa tagccagatc gttgatgaat tctatacatt
      361 gatacagcct gagactcctt ttttctggcg aaatgtccaa attcacggaa tccatcaaga
      421 agacgtacgc aatgcgccga aattcccaga agtctggcaa actatccaac catattttca
      481 aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat acaaaggtct tagcgggatg
      541 cttagattat tatcatcttg aacagcctaa ttatctttct ttatgtacag tgaaaagtag
      601 ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa
      661 catccaatta gatcatcacc atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct
      721 gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa aaacttgtga ctataaagta
      781 aaagaatact tatgaaacac acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca
      841 aggagcaatt tcaaaaaata gcttctcatg tttattaaat attgtgactg gtgaccgagg
      901 agctggcagg agaacaccgt tta
//
LOCUS       DACEPW010000399          915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.416, whole genome shotgun sequence.
ACCESSION   DACEPW010000399 DACEPW010000000
VERSION     DACEPW010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.416"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(277..732)
                     /gene="tnpA"
                     /locus_tag="IW819_002930"
     CDS             complement(277..732)
                     /gene="tnpA"
                     /locus_tag="IW819_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1245999.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          247 a          188 c          150 g          330 t
ORIGIN      
        1 attatttctg cgccagcatt ttctaatgtt tgaatttgtg ttttgtaatc ttggttgctt
       61 gtgctaattc ttgaatagcc gtaaattgcc aaaatttcat caccctttct ggacataagt
      121 ttctgacacc agttaaaatc aagtctatca gtaacccata cacccgtgag gacgggtgta
      181 tttttaatcc ccctagaagg ggtcgacact cgctgcccct taaaagggga tttttaaaag
      241 accgccaact gctcatcttc actcacttac ttctctttat tttttaaatg gttctacgta
      301 ctctctttta cttataatat atcgaatgcg atcatcagct tcttgttcac ggatgtattt
      361 ttcatttgtc ttttgattca atccgactgt actcacatag tatctcttcg accaaaagga
      421 tttgtttccg tatttatatt tgagatttgc gtgctgctcg tggattagta ccgcactacg
      481 acctttcaga taccccataa aactggatat acatattttt ggtggtactc gaacaagcat
      541 gtgaatatga tctagcattg catgtgcttc tatgatttcc acatctttca tttcgcacaa
      601 cttcctaagt atttttccaa gatcttttcg taattgccca tagattactt ttcgttgata
      661 ttttggtgta aaaataatat gatacttaca attccaacga gtatatgcta aacttttatc
      721 gtcatttgac atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc actcactagt
      781 atatcgaaag gagtttttat cgctaaagat acttcttcca cgcgtagaaa cgcgcggttt
      841 tcttgtttcg cacgctttac gcacaaaact gactgaagtc attaagaaaa taaaagggtc
      901 taaaactttt aagtt
//
LOCUS       DACEPW010000400          907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.417, whole genome shotgun sequence.
ACCESSION   DACEPW010000400 DACEPW010000000
VERSION     DACEPW010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.417"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..734
                     /locus_tag="IW819_002931"
     CDS             168..734
                     /locus_tag="IW819_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1246000.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT          337 a          123 c          178 g          269 t
ORIGIN      
        1 ggaacccatt gaatttaacc aaattttttt aattttgcaa aaaaaagtta aagaagactg
       61 aaaaaaaatt taaaatataa cgttttgagt aaaatgtttg caaaaaatgc taaaaatagg
      121 gtacaataaa acaacatata ctgcagtaaa gaagtggggg agttttaatg agaagaaaag
      181 tctataccaa ggatcaaatt ctaaaagctg cttatgatgt tgttgcaaag gaagggttta
      241 aaggatttac tgcgagaaat attgctaaaa agatggggat ttcgacacag ccgatttatc
      301 tggaattcaa aaacatggaa gatttaaaaa atacgttgct ggattctatc tttgaacaac
      361 taaaagaaga tgttttccct attgaacgta ctggtgataa aatcattgat ctcggtttga
      421 actatatcta ctttgcaaaa aagcatcata gtctttacat ggcattgtac attgatgaac
      481 atggggacgg aaagaaaatg catgattttt catacaatta tttccgtgaa atagttaaag
      541 aagatgaaag atacaatcaa ttatcggatg aatatgtcga tgctctgcat actggtacat
      601 gggtagcagt gactggctta gcgtcattga tgtcatcaaa catcatgcat ccaacagaag
      661 aacaaatcat tgcactgatc aaacggacaa ttgactcgat tttagcatta gataatcctg
      721 aaataatcag atagtgttct atcagtatga atttgatcgg taagtccgtt ggctgcaaac
      781 tgtcaatgga cttttttgct aaaaaaagcg atttctgtta tttatctatt agaaataggc
      841 ttgacatttc gtgaaaaaag tttaaaaata gtacttacgg aaggttggca gagtggtaat
      901 gcaccgg
//
LOCUS       DACEPW010000401          903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.418, whole genome shotgun sequence.
ACCESSION   DACEPW010000401 DACEPW010000000
VERSION     DACEPW010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.418"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(7..756)
                     /locus_tag="IW819_002932"
     CDS             complement(7..756)
                     /locus_tag="IW819_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1246001.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT          308 a          163 c          164 g          268 t
ORIGIN      
        1 gtttttttat gcgtgttgtt gtgcgttgta gtattcttct tccatttttt gcatacgttc
       61 acgttctttt tggttatacc aaggtgaaac ggtaaatgca aggacatcat tcagcaagta
      121 aacagagctg ttcacgaaca tcgccaaact tgcagatcca tgcgtaaatg aaatatacca
      181 aaggaccatt tgagcaagcc ctgaagctaa ccaccagtaa tattggttgt tgtaacgcaa
      241 gaaactgatg ataccagcag tcaagctaat agaaaaagca atggcatcta cccacggccg
      301 aggatcatct gtataacgac caatcaggaa tcctgaaaca agatagacaa ggaaagtact
      361 gatgattgcg ataaaccagc tttttccagt gaattttcgg atttttgaag ccatattatg
      421 attccattct ttacttaaca atacggggat atccaatgtg atcatataag caatctgttc
      481 accgatactt aaatagttct ttgctgaaaa cccaacgata ataaaacaaa tcgcagacaa
      541 aatacctaaa ataccgttga ctgattttgc cgcattgatg gataaaatac acaatacacc
      601 aagcgtggta ccgataaatg taataacgga cagccaagtg atgggactat tgacaagagt
      661 catgacctgg cagccaagac tgaaaaagaa aagataataa ttttgttgcg gccagccttt
      721 tagctgatga gctaaaaact gaaaatatga cttcatgtat gaggactcct taagacacta
      781 aatgtagtgt gttctgtatt tactaacaca atctattgta tgcttttctt gaaaaaaaac
      841 tagtctttca attataaata atttgtgtta tgataatttt ctgtttgttt tcttgaaaat
      901 ata
//
LOCUS       DACEPW010000402          889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.419, whole genome shotgun sequence.
ACCESSION   DACEPW010000402 DACEPW010000000
VERSION     DACEPW010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.419"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>889
                     /locus_tag="IW819_002933"
     CDS             <1..>889
                     /locus_tag="IW819_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1246002.1"
                     /translation="NSNVLDALDSVDELETTLDQSKESMYKLSYVVRVSAESVDELKR
                     RCDEVLDFYDDTNVKLVRPFGDMMGLHEEFLPSSKRYMNDYIQYVTSDFLAGLGFGAT
                     QMLGELEGIYFGYNVDTGRNVYLKPALASQGVKGSVTNALAAAFLGSLGGGKSFSNNL
                     LVYYAVLFGGQAVIVDPKGERGGWKETLPEIADEINILNLTSKPKNQGLLDPYVIMEK
                     KKDSESLAIDILTFLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPV
                     AENIADHIESFTDYDFAHLL"
BASE COUNT          254 a          163 c          216 g          256 t
ORIGIN      
        1 aattccaatg tcttagatgc attggattcc gtagatgaat tggaaactac cttggatcag
       61 tccaaagaat ccatgtacaa actgagctat gtggttcgag taagtgccga atcagtagat
      121 gaactaaagc gccggtgtga tgaagtgcta gatttttatg atgataccaa tgtaaaacta
      181 gtccggcctt ttggcgatat gatggggcta catgaagaat tcttaccttc tagtaagcga
      241 tacatgaatg attatatcca gtatgttacg tctgattttc tcgccggact tggttttggc
      301 gccactcaga tgttagggga gttggaaggc atttactttg gttataacgt agatacgggg
      361 cgcaatgttt acctgaaacc ggcattggcc tcacaagggg tcaagggttc agttaccaat
      421 gcgctagcag cggcattcct cggttcactc ggtggcggaa agtcctttag taacaatctc
      481 ttagtttatt acgccgtcct ttttggtgga caagctgtga ttgtcgatcc aaaaggggaa
      541 cggggtggct ggaaagaaac cttgccagaa attgcggatg aaatcaatat tctcaactta
      601 actagtaagc caaaaaatca aggattactt gatccttatg tgattatgga gaaaaagaag
      661 gattctgaaa gtctagcaat tgatattctg acctttttaa caggaatttc tagtcgagat
      721 ggggagaagt ttccagtgtt gcgtaaggcc attcggacgg ttacccaaag caatcaacgg
      781 ggattattac aagtaattaa tgagcttcga aaagaaggct caccagttgc ggaaaatatc
      841 gcggatcaca tcgagtcctt taccgattat gattttgccc atttgcttt
//
LOCUS       DACEPW010000403          884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.420, whole genome shotgun sequence.
ACCESSION   DACEPW010000403 DACEPW010000000
VERSION     DACEPW010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.420"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>884
                     /locus_tag="IW819_002934"
     CDS             <1..>884
                     /locus_tag="IW819_002934"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1246003.1"
                     /translation="GQDMICIYVAIGQKDSTVRTQVETLKKYGAMDYTIVVNAGASQP
                     APLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLLLRRPPGREAYPGD
                     VFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNVISITDGQIFLESD
                     LFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYRELEAFTQFGSDLDAA
                     TQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPVDSILDFEHELFEY
                     LDTNHSDIFETIRTTKDLP"
BASE COUNT          264 a          180 c          182 g          258 t
ORIGIN      
        1 ggtcaagata tgatctgtat ctatgtagca atcggacaaa aagattctac agttcgtaca
       61 caagttgaaa cattgaaaaa atatggcgca atggattaca caatcgttgt gaatgccggt
      121 gcgtctcaac cagcaccatt actttatatc gcaccatatg ctggtactgc aatgggtgaa
      181 gaattcatgt acaacggtaa acatgtattg atcatttttg atgatctttc aaaacaagct
      241 gttgcttatc gtgaactttc cttgctactt cgccgtccac caggtcgtga agcttatcct
      301 ggggatgttt tctacttgca ctcacgttta ttagaacgtg cagcaaaatt aagtgatgat
      361 ctaggcggcg gttcaatgac tgctttgcca tttgttgaaa cacaagctgg agatatctct
      421 gcttatatcc caacaaacgt tatctcaatc actgacggac aaatcttctt agaaagcgac
      481 ttgttctacg cgggtactcg tccagcggtt gatgccggac tatctgtctc tcgtgtaggg
      541 ggttctgcgc aaatcaaagc aatgaaaaaa gttgctggga ctttacgtct agacttagca
      601 agttatcgtg aattagaagc atttacacaa ttcggttctg atttggatgc ggcaacacaa
      661 gcaaaactaa atcgtggacg ccgtacagta gaaatcttga aacaaaaact gcatgcaccg
      721 ctagctgttg agaaacaagt agtgattttg tatgccttga cacatggatt cttggacagt
      781 atccctgtag acagtatttt agacttcgaa catgaattat tcgaatatct ggataccaat
      841 cattcagata tttttgaaac aatccgtaca acaaaagatc ttcc
//
LOCUS       DACEPW010000404          871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.422, whole genome shotgun sequence.
ACCESSION   DACEPW010000404 DACEPW010000000
VERSION     DACEPW010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.422"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..255
                     /gene="pyrH"
                     /locus_tag="IW819_002935"
     CDS             <1..255
                     /gene="pyrH"
                     /locus_tag="IW819_002935"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1246004.1"
                     /translation="VILMAKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDS
                     TASSLSMDNDIPLLVFNLNEHGNIRRAILGENIGTTVRGK"
     gene            258..815
                     /gene="frr"
                     /locus_tag="IW819_002936"
     CDS             258..815
                     /gene="frr"
                     /locus_tag="IW819_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1246005.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT          331 a          152 c          178 g          210 t
ORIGIN      
        1 gttatcctga tggctaaaaa caatgtagac ggtgtttatt ctgctgatcc gaaagtagat
       61 gcaaatgcag taaaatttga agaactgact catctggaag ttattgccaa aggattacaa
      121 gtcatggatt caactgcaag ttcactaagc atggataatg atattccact gcttgtcttc
      181 aatttaaatg aacacggaaa tattcgccgt gcaatcttag gcgaaaatat cggaacaact
      241 gtaaggggga aataaaaatg gcagatgcaa ttatgacaga agcaaaagaa aaaatgaaaa
      301 aagcagcaca aaatcttcaa cgtgaacttg ggcaaatccg cgcaggccgt gcgaacgcta
      361 gccttttgga ccgtattact gtgaactact acggtgcgcc aacaccattg aaccaaatgg
      421 catcaatcca aattccagaa gcacgtgttt taatgatcac accttttgac aagagcattc
      481 ttcaagatgt tgaaaaagct attatggcaa gtgatatcgg aatcagtcca accaatgatg
      541 gaaatgtcat tcgcttagtt atcccgcaac tgacagaaga acgcagaaaa gaattagcta
      601 aagatgtgaa aaaagaagca gaaaatgcaa aaatcgcgat tcgtaacatc cgtcgtgatg
      661 cgatcgatga atataaaaaa caacaaaaaa atggtgatat taccgaagat gatttgcgcg
      721 gattagaaaa agacgtacaa acattgacag ataacagcat caaagaagta gataagatcg
      781 ctgctgataa agaaaaagaa ttattggaag tttaataaaa tcataaaaac agaggggcgg
      841 atcattccgc ttcctctgtt tttatttata t
//
LOCUS       DACEPW010000405          864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.423, whole genome shotgun sequence.
ACCESSION   DACEPW010000405 DACEPW010000000
VERSION     DACEPW010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.423"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..554)
                     /locus_tag="IW819_002937"
     CDS             complement(<1..554)
                     /locus_tag="IW819_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1246006.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNL"
     gene            complement(575..>864)
                     /locus_tag="IW819_002938"
     CDS             complement(575..>864)
                     /locus_tag="IW819_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1246007.1"
                     /translation="DPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELMNVGNAGKMTPP
                     SEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISILSDF"
BASE COUNT          266 a          215 c          150 g          233 t
ORIGIN      
        1 agatttccag cgactgttaa tccgttcatg atgacctctg gaatattatc caagacgctt
       61 ttgacaacat ctgctccaaa taaaacggca aagaatggaa taagagcata aagcgcatag
      121 tggacaaacc acataaagaa gtgcagattg atgattcctt tttgatcacc agattttgca
      181 aatttgaaaa atttagcagc catcggtcca catacaaaag aaaaaatcat attcattaac
      241 tgtaatccta atactgctag tggaaagaca atgggaataa tcgcatccac gtttcctccc
      301 attttgattg cgaacgctgt agccagccca gcagccaaag aaggctccgc cgtactagag
      361 attccgatat tgacgacacc cataaagatg gcttctagtg acgccccgac aatcaacccc
      421 gtctgcaaat cccccatcaa cgctcctgct aacggagcaa caacaatcgg acgactcagc
      481 atggacatcc cgaacatttc atgtccagca actccaataa aaacggccaa agcgactaca
      541 aatgcatcat acataatttt tctccttcct ttttctaaaa atcactcaaa atagagatag
      601 ctgaacgttt ttccatcgcc ggtgtaggct gaaaaacggt atctggataa agttcaacta
      661 attcttttaa gtcttgaatt tctgacgtcg ttaacatcac ttcctttgat aaggttttct
      721 tatcttccga tggcggtgtc attttcccag cattccccac gttcattaat tcgatttccg
      781 ttgggaaagc tttcgcaact tttaccgcat ctttgacatt agggactaaa accatcaagc
      841 gcatgtcctt cgcccgcgga tctt
//
LOCUS       DACEPW010000406          861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.424, whole genome shotgun sequence.
ACCESSION   DACEPW010000406 DACEPW010000000
VERSION     DACEPW010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.424"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            178..585
                     /locus_tag="IW819_002939"
     CDS             178..585
                     /locus_tag="IW819_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1246008.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAI
                     KYAIVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESTDYYIY"
BASE COUNT          292 a          119 c          177 g          273 t
ORIGIN      
        1 catccgttgt ttggtgaacg cattaataaa ctagttttta ttggtatgaa gatggatcaa
       61 caatgtatta aggaacaact tgagggttgt ttaactgatt ctcttaatgc agataatttc
      121 cgaaaaattt caccatttcc atggaaaaag tctaatatgt ttagccagat atcgttaatg
      181 aaatttttaa gaaaagctgg tgtcaagata ggcgatgtca taattattgg cattttgatt
      241 attacctctt ttattccctt atttgtattc tctatagaaa aggagaatga aacaactgaa
      301 gatgctatca aatatgccat cgttcgaatt gatggcaagg aaatggatcg cttcgatttg
      361 gataagatca atcataaact cgtgaattat catcctgcga agggacaata taatattgtt
      421 gagattaaag atggaagaat tcgagttaaa gaagataata gtcctgatca aatcgcggtt
      481 agaactggat ggatctcaaa acccggtcaa acgagtattt gtttacccca taagttagtt
      541 attagtattg agaaaaaaga aagcacagat tactacattt actaatctgg aaatagatga
      601 ggaggttttt aataagaaaa agatattatt attaataata gcaggggctt tattaagtgt
      661 gttaagcgct tgtggacaag aaaaagaagc ggtgacaaca ggcacaagta tctttaacgg
      721 gacctgcaat ggtttagtta acagtcggtt agattactta ttttggctct tcgtcaatga
      781 gtgttggtga agaaattcaa tagaataatc gagatactca cacggctaga cagctatctt
      841 gtctagccgt gttgttgttc t
//
LOCUS       DACEPW010000407          847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.426, whole genome shotgun sequence.
ACCESSION   DACEPW010000407 DACEPW010000000
VERSION     DACEPW010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.426"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..523
                     /locus_tag="IW819_002940"
     CDS             <1..523
                     /locus_tag="IW819_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1246009.1"
                     /translation="TFTHGDVVDMFDDVSITHDVLDGSKEAFETILYALLPDGTNKEI
                     WKSGKIDYEVNDKEFTKTVLAKKVDTGKYPEGTKFTFAEINYDKDGNINGKHNEDLKE
                     KSQTLTPKEVPTILSTPKQPETPTVPSGSQESSPTVKTFPQTGEKNSNVLLFIGFTLI
                     FATAGYYFWNRRN"
     gene            636..>847
                     /locus_tag="IW819_002941"
     CDS             636..>847
                     /locus_tag="IW819_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1246010.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQP"
BASE COUNT          296 a          145 c          190 g          216 t
ORIGIN      
        1 gacctttact catggtgatg tggtggatat gtttgatgat gtgtcgatta ctcatgatgt
       61 actggatggc tcaaaagaag ctttcgaaac aattctgtat gctttattac cggatggtac
      121 aaacaaagaa atttggaaat ctggcaagat tgattatgaa gtaaatgata aagaattcac
      181 caaaactgtg cttgcgaaaa aagtagatac cggaaaatat ccagaaggaa ccaagtttac
      241 ttttgcggaa atcaattacg ataaagatgg gaatatcaat ggaaaacaca atgaagattt
      301 gaaagaaaaa tctcaaacct taacaccaaa agaagttcca accatactaa gtacgccgaa
      361 acaaccggaa acaccaactg ttccaagtgg ctctcaagaa tctagtccca cagtgaagac
      421 cttccctcaa accggggaga aaaattccaa tgttttactg ttcattggtt ttactttgat
      481 ttttgcgacg gcgggctatt atttctggaa tcgccggaac taaggtgatg agatgataca
      541 agtaaatcct aagaaccaac gtgcgcgacc gcctcctaaa aaagtagtcg cgctattttt
      601 gtatccaaaa ctaacaatga aaagaggaaa agaagatgga attgaaattt gtcgtgccag
      661 atatggctga aacctttgga aagttaagtt atgccggtga aggagaaatt ataacagaag
      721 gatatggacg gaatactacg gtgattggtc gcagttatca tttgtattcc agtaagcaac
      781 gggccgatga tattgaggtg gtagtagctg cggaagccgg tgaaaaggat tttgatcaag
      841 accaacc
//
LOCUS       DACEPW010000408          838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.427, whole genome shotgun sequence.
ACCESSION   DACEPW010000408 DACEPW010000000
VERSION     DACEPW010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.427"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..717)
                     /gene="msrA"
                     /locus_tag="IW819_002942"
     CDS             complement(<1..717)
                     /gene="msrA"
                     /locus_tag="IW819_002942"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1246011.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQF"
BASE COUNT          227 a          175 c          142 g          294 t
ORIGIN      
        1 aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga tttttgtgac
       61 gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt ccgctaattc
      121 tttttgtcgt tcgacttgat aaaaaatcac tggacgatat tgcttccctc tatcttgaaa
      181 ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca actcgctata
      241 acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg tgtaaccacc
      301 acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg acaaaacaga
      361 taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc cccctgcaaa
      421 tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt catatttttg
      481 ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag gcgtcagatt
      541 cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt ctttaacgga
      601 tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc aatctcgtgt
      661 attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct catccataga
      721 ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat cataaagtga
      781 ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga gagaatcc
//
LOCUS       DACEPW010000409          818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.429, whole genome shotgun sequence.
ACCESSION   DACEPW010000409 DACEPW010000000
VERSION     DACEPW010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.429"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          318 a           93 c          137 g          270 t
ORIGIN      
        1 aaaattggat cagttaattg aaagagaaag ctgggaagat tattacgtgg gtctaacttt
       61 ttcagaaaag ttaaacaact acaagttaat agattataag tatttgagtg aaagcaatta
      121 attattcatg tagagtacta gacactttaa atatggacaa ttcaattata aagaaaaaga
      181 atcaataatt taatgaggct gtgacaaatg tcattttttg tttcgctaaa aacgagtgaa
      241 aaaatgttga taactccaat cattcgttta agattctagg aatcattcag aaatgctgtg
      301 aaaccgatac tttataatta taaagaaggt gatttcaaat tccgaagaag gaatttgaag
      361 tcaccttttt acttatgtca cagtctcatt cagtaatgcg ggataaggct ccttattccg
      421 ttatttgaat actctgaagt ttaatctaag aaagggtaat attttgaaaa catgcttcat
      481 cgcatagaaa tgaacttaat ttcagaaaga atacatacat atctttttat atgttttcag
      541 ctgctgaatg gttaaaaagc acaaatatcc gaaaaaaaca tgaagaaatg tacttttttt
      601 gttgtaatgt tcatgaaatt ctctaagtaa attagttaac ataaggttag taagattcct
      661 attctgtgaa taatacgtat cagatttatc ttttattctt actaggaaag taattattaa
      721 gggagttgat ttgaataatg gttagcaaga ataataagag agtatttcta gaaaaaacaa
      781 agaaacgagt attgaagtac agtatcaaaa aattaagt
//
LOCUS       DACEPW010000410          812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.431, whole genome shotgun sequence.
ACCESSION   DACEPW010000410 DACEPW010000000
VERSION     DACEPW010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.431"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(162..647)
                     /locus_tag="IW819_002943"
     CDS             complement(162..647)
                     /locus_tag="IW819_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1246012.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT          280 a          131 c          143 g          258 t
ORIGIN      
        1 tcaagagagc aagtagagaa gtgagtatat tcataagaga aatagctgta acgaattatt
       61 aggttaatgc aaatgcttat ttatactccg tataactttc tcagaaaaaa tgataccaat
      121 aatgacttgc tagtactgta aatccgtata atatcatcta atcaaaatgg tgttccattt
      181 gcatctgtca tcgtaatcga tccttcgggt gacactcgat aaaaaccaac gcttccagat
      241 cctccttggg aacggatttg tttgctggaa gctgtgaaca aataatctcg atcaattttt
      301 tgataaaagc taataacaat atcgtcactt tgtaattcgg aacggtgggc aataagaaat
      361 agaagtgctt tgtcagagtt ttctaatgga agagaaggta cagtgctttc ttgcatttca
      421 gatgttgaag aaatgctaaa cgactgaata cttgattggc tatgttgcga atctacatta
      481 ccagtagaag tattagtaat ggaagtagta gaagtacttt tagttgaacc aacctcagtt
      541 gttgaaatgg ctgctgaaga ttctttaaca gaattctttg ttttatatcc acaggcagaa
      601 aaaagtatta cagttcccaa caagcctgcg taaatccctt tatgcatgat agctcctcct
      661 aaaatagatt atctttattc agtatatcac ttaaacaaag aaaagcagat ggtttaaaat
      721 gaaaaaacct tcccaataaa gggaaggttc atgaagataa taactttttt aataattata
      781 gctttctaat atctttcatc aacttttcta gt
//
LOCUS       DACEPW010000411          800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.433, whole genome shotgun sequence.
ACCESSION   DACEPW010000411 DACEPW010000000
VERSION     DACEPW010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.433"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            375..773
                     /gene="spxA"
                     /locus_tag="IW819_002944"
     CDS             375..773
                     /gene="spxA"
                     /locus_tag="IW819_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1246013.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          302 a          110 c          140 g          248 t
ORIGIN      
        1 aagcgttata ataaagaaca gtattattta aaatgacaga aaacattact attaaagaaa
       61 taaaaaacat aaaaaatctt aagaaatcgg tttcatttag cttttgagat aaaataatag
      121 tacagtttta tacgctgttt catatgttta gaaaatttga attagattta aaactctaat
      181 ttttaaatga taaaaaaagc gagaatgctg gttttactgt atattaacct tagataactt
      241 tcattttttt aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat tatgtataat
      301 agaaacagat gggagattat tatgatcttc tgttacaaaa aagtgattct tatcacgaaa
      361 aggagtggtg cacgttgtta acattatata cttccccaag ttgtacttcc tgcagaaagg
      421 ctcgtgcgtg gttgcaagaa catcagattc catttgttga acgtaatatt ttttcagaac
      481 cattgaacag ttctgaatta aaagcgattt tacaaatgac agaagacgga acagaagaaa
      541 tcatctcgac acgttccaaa gtattccaaa aactgaacat ggatttagat gatctgccgt
      601 tacaagaatt attggaactt gtgcaaaaca atcctggatt gcttcgccgt ccaatcatga
      661 ttgatgataa acgtctgcaa gttggtttta atgaagatga aattcgtcgg ttcctaccgc
      721 gcgatgtacg acaattagaa ttacgtcaag cacaattgat ggctggatta tagaaggata
      781 atacgaaact tggaaaagac
//
LOCUS       DACEPW010000412          795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.434, whole genome shotgun sequence.
ACCESSION   DACEPW010000412 DACEPW010000000
VERSION     DACEPW010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.434"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>795
                     /locus_tag="IW819_002945"
     CDS             <1..>795
                     /locus_tag="IW819_002945"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1246014.1"
                     /translation="KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNL
                     AEYGIEVICYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNE
                     FGLPGWEPERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDP
                     PYSIFGLARIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDR
                     IPFAHVRNIKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYI"
BASE COUNT          260 a          150 c          164 g          221 t
ORIGIN      
        1 aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt caatgtgcat
       61 gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta taagcagaca
      121 atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat gcctgtattt
      181 gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac gttagctttt
      241 atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga acattccagc
      301 aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa atccttgctt
      361 gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta ttttctccaa
      421 tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc agatgatccg
      481 ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt agattggttg
      541 tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag tattgcagaa
      601 gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga tcgaatccca
      661 tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga ggcaccacat
      721 aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta tgacaacgga
      781 tttaacggat acatc
//
LOCUS       DACEPW010000413          709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.444, whole genome shotgun sequence.
ACCESSION   DACEPW010000413 DACEPW010000000
VERSION     DACEPW010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.444"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..526)
                     /locus_tag="IW819_002946"
     CDS             complement(<1..526)
                     /locus_tag="IW819_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1246015.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPS"
BASE COUNT          283 a          114 c          116 g          196 t
ORIGIN      
        1 cactaggagc cataccactt aattcttttc ccagtgggac tttcaattct tgtgctctag
       61 attgttgaat aaaaggaatt aagaatcccg cacttaaaaa aaaggtgagg acggtagctt
      121 ttaaaagact taagagtcta gcgatttttt ggtcccaaaa ataaaaagaa agaaacaacg
      181 ttcctccaat aaatacagaa accatagcga cagaaagtaa atgagtatac actactagcg
      241 tcatgccaat agacaaccag taccattttt ggtaatctct aatataaatt tcgtaacacc
      301 ccattagtat caaaggcaaa aatgttaaag cgacagcttc tcccaatgaa gcacgatgga
      361 atatgtcaat ggcacgatag gcggaaaatg tataaataat cgaaaataac agcgaaatat
      421 aacgattgtt ttttatttgt ttcatagaaa aataagaaac gaccattgtt aaaaaagtga
      481 taaaaaaata gtagatatta taacttaaaa caagatttcc catcatttta taaaataaaa
      541 aagctggata aagagtaagc catggataaa aaatattcat cattgttcca tgatgatcga
      601 agttattgaa gtttactgga gaactccata cattatctag tcctatgatt cgagataaat
      661 gaaagtaagt atcctgattg acactatgat agattccctt agacatgaa
//
LOCUS       DACEPW010000414          704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.445, whole genome shotgun sequence.
ACCESSION   DACEPW010000414 DACEPW010000000
VERSION     DACEPW010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.445"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..309
                     /locus_tag="IW819_002947"
     CDS             91..309
                     /locus_tag="IW819_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1246016.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(598..>704)
                     /locus_tag="IW819_002948"
     CDS             complement(598..>704)
                     /locus_tag="IW819_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1246017.1"
                     /translation="LGGVVVGCAFLIELMDLHGRDKIKGYDIITLMEY"
BASE COUNT          214 a          124 c          123 g          243 t
ORIGIN      
        1 ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata tacgaacaat
       61 tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct tggcttagta
      121 ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc agctttactt
      181 gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa gtaccgtcaa
      241 tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta cagatccagg
      301 caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa cttttacggg
      361 ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta aagccagttt
      421 ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg gtctttgctt
      481 gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct gacttttgag
      541 ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt tttctgctta
      601 atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat ggagatccat
      661 caattcgatc aaaaatgcgc agcctacgac tacaccgcct aatt
//
LOCUS       DACEPW010000415          695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.446, whole genome shotgun sequence.
ACCESSION   DACEPW010000415 DACEPW010000000
VERSION     DACEPW010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.446"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>695
                     /locus_tag="IW819_002949"
     CDS             <1..>695
                     /locus_tag="IW819_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1246018.1"
                     /translation="TLLYDMIANRIGIDEVVAENGTLRLMKNQVWAYDSLPHMLIAGG
                     TGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQKEEISACVEDFY
                     ERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTKESAVILNKLKQ
                     IVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYSMMFGEVEKNFF
                     MKHIKGRGYVDTGG"
BASE COUNT          203 a          116 c          171 g          205 t
ORIGIN      
        1 actttactgt atgatatgat cgccaatcga attgggatag acgaagtagt ggcagaaaat
       61 gggactctgc gattgatgaa aaatcaagta tgggcttatg attctttacc ccatatgtta
      121 atcgctggtg gcacgggtgg agggaagacc tatttcctcc tcaccattat cgaagcacta
      181 ttgaagtcag atgcagaatt gtttatcctc gatcccaaaa atgcagattt agctgattta
      241 ggtacggtga tgcctcatgt ttattcacaa aaggaagaaa tttctgcttg tgtggaagat
      301 ttctatgaac gtatgatggc tcgtagcaag gcaatgaaag aaatgtctaa ctacaaaaca
      361 ggagagaatt atgcctatct tggacttcca cccaactttt taatctttga tgaatacgtg
      421 gcttatatgg agatgttaac gacgaaagaa agtgcggtga ttttgaataa gctcaaacaa
      481 atcgtgatgt tgggtcgtca atctggtttc tttctgattt tggcttgcca aaggccggat
      541 gcgaaatatt taggagacgg aatccgcgat caattcaact ttcgcgtggc tttgggtcgt
      601 atgagtgaac tcggatattc catgatgttt ggggaggtcg aaaaaaactt ctttatgaag
      661 cacatcaaag gtcgaggtta tgtggataca ggtgg
//
LOCUS       DACEPW010000416          691 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.447, whole genome shotgun sequence.
ACCESSION   DACEPW010000416 DACEPW010000000
VERSION     DACEPW010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.447"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..605)
                     /locus_tag="IW819_002950"
     CDS             complement(<1..605)
                     /locus_tag="IW819_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1246019.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDEL"
BASE COUNT          224 a          134 c           98 g          235 t
ORIGIN      
        1 atcaattcgt catatcttcc aaaatcaaca ttgtactcat aaccaagaag tgtcggaatc
       61 aggtcaattt caatttcttt ttcaattgaa cgtttaataa gtttcgtgga tacatacccg
      121 acaaaaaaga gaataatacc cacttcaaac aaatacatgt gtccatttac aaagtgaata
      181 aacgtcaata aaaaatcctt cactcctttt gttgttcttc cagattgttc aatttgtttt
      241 attaaagtcg ctagaattgc atttacaaca attaaatcga ataggctttt gaaaagtttt
      301 cctgctgtta atttttgcct ggtaaattgc atgactaatt gagtaaaaat agacaatagc
      361 atgacaaaaa tcaacgtcat tagcactgta aaaatgtctg atgctgcaat cctattggga
      421 tcatttatca agtaataagc cgttccaaat actccaatcg agtaaacggt taaatcaaga
      481 ataagtcgtg ccccgtaact acaaagtttt cctaatggcc ataattgcaa tacccaaaat
      541 ttctttcgtt tgctatttct tttcagagga agccaggtgt gttcaatcca gtcgtccgat
      601 ttcatttcga tttcttccct aaattctttc ctttaacgtt ttcctttgct actttagcag
      661 cgataggttg ctcaatgaac caatttttta a
//
LOCUS       DACEPW010000417          684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.448, whole genome shotgun sequence.
ACCESSION   DACEPW010000417 DACEPW010000000
VERSION     DACEPW010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.448"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..684
                     /locus_tag="IW819_002951"
                     /pseudo
     CDS             1..684
                     /locus_tag="IW819_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          251 a          123 c          145 g          165 t
ORIGIN      
        1 taagtatact tttgaactta aattaaagat cgttcatgat tacttggatg gtaaaggcgg
       61 aagtgattat ctggcaaaga aatattccat taaggctcca tcacaagtga agcgctggat
      121 caatgcctat caagaatttg gtgaagaggg tctagtaaga aagcgacaaa agaaaaaata
      181 ttctgttcaa tttaagctgg atgcgataga gttatatcta acaagtgagc tgtcctatcg
      241 tgaagttgcc aacacactta acatgaacaa ccctaatttg atcgctagtt ggatgcgtca
      301 attccgtgag ggtgggatcg atggactctc aaaaacgaaa ggatgtccac ctatcttgtc
      361 taagaaaaat gaacccaaaa ataagaaaaa atctacaact aaagcaacgt ctaaagaacg
      421 tgaacgcatc gaggagttgg agaaacgagt ccgttccctt caaattgaaa atgccttttt
      481 aaaagaattg aggaagctgc gaaaacagga agcccaacaa cgacgaatga agcaatcgca
      541 cgaatcatcg caagcctccg aggaccgttt aaattagttg agcttctaga aactttaaag
      601 ttccctaagg caatctttat gtactggcaa aaacgattgg accgtaaaaa tccagatcaa
      661 gagattgaag cggaaatgct taaa
//
LOCUS       DACEPW010000418          667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.453, whole genome shotgun sequence.
ACCESSION   DACEPW010000418 DACEPW010000000
VERSION     DACEPW010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.453"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>667
                     /locus_tag="IW819_002952"
     CDS             <1..>667
                     /locus_tag="IW819_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ1246020.1"
                     /translation="KAIEKVPLDLGWKKLFGKESDGKEKEEEPLLPKVTKGEMVTVDL
                     QVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLK
                     QKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGERKGTQTTFL
                     ANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYQ
                     KVAA"
BASE COUNT          249 a          122 c          166 g          130 t
ORIGIN      
        1 taaaggcaat cgaaaaggtg ccgctagatc taggttggaa gaagttattt ggaaaagaga
       61 gcgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg
      121 tgacggtgga tctacaagtt cttgaaaaag aaaccaagcc accacaacct tataccgaag
      181 gtacgttgat cactgcaatg aaaacagctg ggaaaaccgt ggatagtgaa gaagcgcaat
      241 ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa
      301 ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag
      361 ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacgagcgcc gaaatgaccg
      421 ccaaatggga aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc
      481 tagccaatat ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat
      541 ccactgattt tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg
      601 cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg
      661 tggcagc
//
LOCUS       DACEPW010000419          645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.455, whole genome shotgun sequence.
ACCESSION   DACEPW010000419 DACEPW010000000
VERSION     DACEPW010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.455"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            121..>645
                     /locus_tag="IW819_002953"
     CDS             121..>645
                     /locus_tag="IW819_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1246021.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPPKKSKAKP"
BASE COUNT          264 a           87 c          125 g          169 t
ORIGIN      
        1 attcatcaca cggagaaaca caaaataggt gtggctaatt tcaaaaataa gtatcaaatc
       61 aaagggatta agttaaacca ggaaacaatt aagaaactta gaataggagg gtaaaaaata
      121 ttgtctgaca acaaacgcta ctactattta aaactaaaag agaatttttt cgatagtgac
      181 gagatggttc tcttagaaag tatgccagat ggctatattt attctaatat tcttctcaaa
      241 ctttatttaa gaagtctaaa acacgaaggt aagttgatgt ttaatgacag gattccattc
      301 aattctacaa tgcttgcgac tattacaaga cactctgtgg gagttgtaga aaaagcagta
      361 caaatatttc gtgatttaca acttattgag gtattagata acggagcaat ttatatgtct
      421 gatatacaaa gttttattgg gaaatcttca actgaagctg ataggaagag aaaatacaga
      481 aaagaaattg aggaagcaaa acagaattta ataacaagtg gacaaatgtc ggacaaatgt
      541 ccggacaaaa ctacaccaga gttagagata gagttagaga aagatataga gttagagaaa
      601 gatatagaga atgtaacgcc tccgaaaaaa tcgaaggcta agccc
//
LOCUS       DACEPW010000420          622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.457, whole genome shotgun sequence.
ACCESSION   DACEPW010000420 DACEPW010000000
VERSION     DACEPW010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.457"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            164..>622
                     /locus_tag="IW819_002954"
     CDS             164..>622
                     /locus_tag="IW819_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1246022.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVR"
BASE COUNT          264 a           86 c           99 g          173 t
ORIGIN      
        1 tatataattt tccagtaatc agaattgacg gtatagttcg ctttgacata gaggatatca
       61 aagattttat gaatacattt aaaacaattt gaaaatttct tctcgccgca tatactaaga
      121 atagcgaaat atactgcctt actttttaag ggagtgagga attttgccta caaaactttc
      181 aaatgggaaa tataaaacta atctgaggta tccaaaaaaa ttcagagaaa tcactggaat
      241 tacatcagaa aagtatcaaa aagtatttcc aactaggcaa ttggctatta aagccgaaaa
      301 tgcaatgaaa aggaaaattg aaacagtttt aagagaggag aatgcaaatt cactagaatt
      361 aaaaggaaaa ataaaattta aggaattcta tgaatcaaaa tggcttccta gatatgaact
      421 aggtcagact actagatcaa aaagagctcc aagttacgta acaattagta atactaaaga
      481 tatattccga ttgcacatcc ttccaatgtt tggtgagtat gcaatgaact atctaaattc
      541 aaatactgag ataatatcag atgaattaac aaaaaaatcg aaagaatacg caaatataaa
      601 aattatcaaa ggatatgtcc gt
//
LOCUS       DACEPW010000421          616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.458, whole genome shotgun sequence.
ACCESSION   DACEPW010000421 DACEPW010000000
VERSION     DACEPW010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.458"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>616)
                     /locus_tag="IW819_002955"
     CDS             complement(<1..>616)
                     /locus_tag="IW819_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1246023.1"
                     /translation="VAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVG
                     GLQVFDWRAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEK
                     RKPNSLVVMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLI
                     HSQERIDIRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGV"
BASE COUNT          194 a          146 c           90 g          186 t
ORIGIN      
        1 acaccgttct ctattgtaac aaatttcaat tcaaaaaaca cctgtatcat aaaaattaat
       61 aatttttcag ggatacgcaa atattgagct attgctttta ttttatgacg tatatcaatt
      121 cgttcttgag aatggatcaa tttaaacaat tttgcgtact gttctcttgt gcctacccca
      181 ttcaaatagg cctcatcagg tgaaatcccc atcagataaa cacgattgaa tgaataagac
      241 cgaaaaactt ctttcagatc cgctaaatca tctggacaat ccataacaac tagagagttt
      301 ggttttcttt tttcaattat tgcctttaca gaatctagtc cttcgaaaac aatcgcagct
      361 tcctggataa tctcaggcac gaatttcaac gtcgaagaat caaatgctaa ataaaggcta
      421 ccttccgacg ttcggctctg ttcacggaaa cgttttgccc gccaatcaaa tacttgcaga
      481 ccacccacag aaaaatcaga taccatcagc tgcggtttct tacgcccgtt ccattcattg
      541 atcgaaagct ttcctaccac atctacctga ttattcagca gttcttcttc ttgagtacca
      601 aaaccaaaag cgaccg
//
LOCUS       DACEPW010000422          602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.463, whole genome shotgun sequence.
ACCESSION   DACEPW010000422 DACEPW010000000
VERSION     DACEPW010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.463"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(68..277)
                     /locus_tag="IW819_002956"
     CDS             complement(68..277)
                     /locus_tag="IW819_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1246024.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(288..>602)
                     /locus_tag="IW819_002957"
     CDS             complement(288..>602)
                     /locus_tag="IW819_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1246025.1"
                     /translation="IDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPS
                     ESLSELPLKEIKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQ
                     KI"
BASE COUNT          197 a          108 c           90 g          207 t
ORIGIN      
        1 caaaaaaaaa ataaaaaacc agtcagaaag actggttttt tatttttttg tctatcatca
       61 agccgtttta tcgtttttta ttttcgcttc ttgttttttt catataaaca gaagcaggtt
      121 ttctttcctt atgaagaaga taataataaa gtcccatccc gccaaacatg accaaaaaac
      181 cgatgactga tcgaatagga aaattaggaa caaaaaagag acagacggat aataatgatg
      241 ccggaaccat tattcgataa tagagttgtt tatccatttt cttcacctca tattttttgt
      301 aaagtaatca attgttctaa ttctcggtaa tctaaaaatt gcacacgcag atgctgctca
      361 cctttcttga catcgaattg gatacttcgc cgctttttct ttattcgttt gaaggtaaca
      421 tcagagagtc tatgtttgat ttcctttaac ggtaattctg acagggattc tgaaggaaca
      481 tagattctta aggataaaag atactcttga tatttttcat ctgttacctc tacttctaga
      541 tattccctaa tataataaca ataagcttcg attccattat ccaaagcaac agtcggatcg
      601 at
//
LOCUS       DACEPW010000423          586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.468, whole genome shotgun sequence.
ACCESSION   DACEPW010000423 DACEPW010000000
VERSION     DACEPW010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.468"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>586
                     /locus_tag="IW819_002958"
     CDS             <1..>586
                     /locus_tag="IW819_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1246026.1"
                     /translation="IRSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMV
                     EFCLKYGYGKGMTKKWTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYAL
                     TNKRLIVAQQKMIGNNVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVD
                     RISDSLNEIIYNLKRRNISATNSNTSTISSASEIR"
BASE COUNT          244 a           74 c           90 g          178 t
ORIGIN      
        1 aataagatct ttgaatttag aagatttaga taaggatcca tcagaaaaaa gaaaattaaa
       61 catatctgat catactgcac aaaacaatca tgatataggt aatgatagaa tgagaaccac
      121 tgaagagatg gtagaatttt gtcttaaata cggctacggt aaaggaatga ctaaaaaatg
      181 gacaactcat cattttaact taatatcaga tcaacttaac agtgacgaat acgtaatctt
      241 ctgctttgta ggattgcata attatatctc atcaactaag catgacaata attatgcata
      301 cgcattaact aataaacgtt taattgtagc tcaacaaaaa atgataggca ataatgtaca
      361 aagtattata ttagacaatt taaataatat tagtaaaaaa agaggagtgc tgttgggtac
      421 attaactatt gatactcttg gtaaagtatt gaatgttgca gttgataaag atactgttga
      481 taggataagt gattctttaa acgaaattat ttataattta aaaagaagaa atatttctgc
      541 tacaaattcc aatacatcaa caatttcctc agcttcagaa ataaga
//
LOCUS       DACEPW010000424          570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.471, whole genome shotgun sequence.
ACCESSION   DACEPW010000424 DACEPW010000000
VERSION     DACEPW010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.471"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..526)
                     /locus_tag="IW819_002959"
     CDS             complement(<1..526)
                     /locus_tag="IW819_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1246027.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVI"
BASE COUNT          204 a          130 c           86 g          150 t
ORIGIN      
        1 aaatcacaat ctgcataaat tccctaaaag ataatttcgt caaaccagct actagacaga
       61 tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac tagtactttt ttaatatctt
      121 tcgtttttct tgtcagcaat tcatcaaatt tctgatactt ttttggcgat aaaataagct
      181 ggacaaaggc tacgccatag taacggacaa gcaaaaatcc gattgcttcc ccaatcacta
      241 ggccaataca actatataaa agcccaaatc cattgccaaa catcagcatg cccgctactg
      301 aggagatccc tcccggcaaa ataggaatga tcacttggat cacttgcaac agaacaaata
      361 tcatcactgc atacttgcca aattgctgga taaacgcttg caaagacgtg ctcgatcgaa
      421 aaatcccatg atgataagca taaagaatca acacaaagaa aaaaaacaga ccaataatgg
      481 gcatgatctg aagacatttt tgtaaaactg atcctttttt agacatctat attccccaca
      541 tcttttttca tttattaaaa ctgcctcctt
//
LOCUS       DACEPW010000425          569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.472, whole genome shotgun sequence.
ACCESSION   DACEPW010000425 DACEPW010000000
VERSION     DACEPW010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.472"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>569)
                     /locus_tag="IW819_002960"
     CDS             complement(<1..>569)
                     /locus_tag="IW819_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall protein"
                     /protein_id="HAQ1246028.1"
                     /translation="VLEEGMEFVSFLSDIPSFIDAEVNNGIVRFSANNTIGGPTSFTL
                     SFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKVIKTDENGQRLAGAK
                     FEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYLLDATVHDVFINEYS
                     IEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT          159 a          136 c           88 g          186 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaac
//
LOCUS       DACEPW010000426          568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.474, whole genome shotgun sequence.
ACCESSION   DACEPW010000426 DACEPW010000000
VERSION     DACEPW010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.474"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..143
                     /locus_tag="IW819_002961"
     CDS             <1..143
                     /locus_tag="IW819_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1246029.1"
                     /translation="EYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQKTIKKLNI
                     GG"
     gene            146..>568
                     /locus_tag="IW819_002962"
     CDS             146..>568
                     /locus_tag="IW819_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1246030.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKP"
BASE COUNT          236 a           89 c          104 g          139 t
ORIGIN      
        1 cagagtacac atttgcagca ctctgcagaa tccatcacac agagaagcac aaaataggtg
       61 tgattaattt caaaaataaa tatcaaatca aagggatcaa attaaaccag aaaacaatca
      121 aaaagttaaa tattggaggg taaaaatgac agaacatcga agttattatg cgattatacc
      181 agccaacgta aggtacgaca aaagacttaa accaaatact aagttgttat acggagagat
      241 aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc aatgagtact ttgcagattt
      301 atatggtgtg aataaagaga ccatatcgcg atgggtaagt gatttgatta agtttggata
      361 cttgaatcgg gaaatcattt acaaagaggg taccaatcaa ataatcaata ggtacctacg
      421 aattaatcaa taccctattg acgaaaaacg caatacccct attgacgaaa aagtcaaaga
      481 taataataca tctattaata atacatttaa taatacaaaa gaatatataa gagagttacc
      541 gccttcgaaa aaatcgaagg ctaagccc
//
LOCUS       DACEPW010000427          491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.489, whole genome shotgun sequence.
ACCESSION   DACEPW010000427 DACEPW010000000
VERSION     DACEPW010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.489"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            102..>491
                     /locus_tag="IW819_002963"
     CDS             102..>491
                     /locus_tag="IW819_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1246031.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTE"
BASE COUNT          202 a           84 c          100 g          105 t
ORIGIN      
        1 gaaagcagaa gcagacaagg tgcaagcaac tttgaaagat caatatcaaa agcatcagag
       61 tcaagcgatc ggtgcgatca ttgaaagggt gaaagaaact tatggccgtc actaagatgg
      121 aaaaagtgac cctcatttca gataaaagaa atcaggagag gattttacaa gcagtacaag
      181 gaatccaaaa cgttgaaatc cgggatctgt atcaaaatag aacaaacaac cagtgggtgg
      241 agaagtattt tccagatacc acgattattg ataaagaagc aaaattaagc gctttaggta
      301 atcggttgaa agacatccaa gaagctatcc aattcataca acaccacggc gatagccgtc
      361 aaaagaaact ccatttgaaa cgtcaagaac tttccttaca agatttggaa gccaattatt
      421 ccgaagaaaa tttcataaat aaattgaaag aagtccttga tttaaaagaa aagtgggaac
      481 aactaactga a
//
LOCUS       DACEPW010000428          476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.denovo.494, whole genome shotgun sequence.
ACCESSION   DACEPW010000428 DACEPW010000000
VERSION     DACEPW010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.denovo.494"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>476
                     /locus_tag="IW819_002964"
     CDS             <1..>476
                     /locus_tag="IW819_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1246032.1"
                     /translation="PAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQTM
                     SNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKE
                     VLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT          170 a           93 c           83 g          130 t
ORIGIN      
        1 ccagcctaca aaagaacaaa cgatacattg gaaaccacta ttatccattt attcgaaaaa
       61 ggtgttacga tgtctgaaat tgctgatttg atcgaaaaaa tgtacggtca tcactatact
      121 ccacaaacca tgtccaacat gactaaagtt ctgactgaag aagtaaatgc ctttaaatcc
      181 agagccttaa atgataagta tgtcgctatt tttatggacg ctacttacat tccactaaaa
      241 cgtcaaaccg tatccaaaga agcgatttat attgccattg gtatacgaga agacggcact
      301 aaagaagtac tgagttatgc gattgctcca actgaatcaa catacgtttg gaatgagctg
      361 ctacaggata ttaactccag aggagttcaa gaagtcttgc tttttattac ggacggctta
      421 aaaggcatga aagatactat ccatcaaatt tatcctaaag caaaatatca gcattg
//
LOCUS       DACEPW010000429        21039 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.guided.030, whole genome shotgun sequence.
ACCESSION   DACEPW010000429 DACEPW010000000
VERSION     DACEPW010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.guided.030"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            122..2008
                     /locus_tag="IW819_002965"
     CDS             122..2008
                     /locus_tag="IW819_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1246033.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQVDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2020..2967
                     /locus_tag="IW819_002966"
     CDS             2020..2967
                     /locus_tag="IW819_002966"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1246034.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2987..3514
                     /locus_tag="IW819_002967"
     CDS             2987..3514
                     /locus_tag="IW819_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1246035.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3577..4230)
                     /locus_tag="IW819_002968"
     CDS             complement(3577..4230)
                     /locus_tag="IW819_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1246036.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4362..5204
                     /locus_tag="IW819_002969"
     CDS             4362..5204
                     /locus_tag="IW819_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1246037.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5362..6258
                     /locus_tag="IW819_002970"
     CDS             5362..6258
                     /locus_tag="IW819_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1246038.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6261..6974
                     /locus_tag="IW819_002971"
     CDS             6261..6974
                     /locus_tag="IW819_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1246039.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            6994..7512
                     /gene="msrA"
                     /locus_tag="IW819_002972"
     CDS             6994..7512
                     /gene="msrA"
                     /locus_tag="IW819_002972"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1246040.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7509..7736
                     /locus_tag="IW819_002973"
     CDS             7509..7736
                     /locus_tag="IW819_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1246041.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            7888..8439
                     /gene="lepB"
                     /locus_tag="IW819_002974"
     CDS             7888..8439
                     /gene="lepB"
                     /locus_tag="IW819_002974"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1246042.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8522..9064)
                     /locus_tag="IW819_002975"
     CDS             complement(8522..9064)
                     /locus_tag="IW819_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1246043.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(9301..9699)
                     /locus_tag="IW819_002976"
     CDS             complement(9301..9699)
                     /locus_tag="IW819_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1246044.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            9959..10819
                     /gene="ylqF"
                     /locus_tag="IW819_002977"
     CDS             9959..10819
                     /gene="ylqF"
                     /locus_tag="IW819_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1246045.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            10812..11579
                     /locus_tag="IW819_002978"
     CDS             10812..11579
                     /locus_tag="IW819_002978"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1246046.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            11635..12492
                     /gene="dprA"
                     /locus_tag="IW819_002979"
     CDS             11635..12492
                     /gene="dprA"
                     /locus_tag="IW819_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1246047.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            12614..14692
                     /gene="topA"
                     /locus_tag="IW819_002980"
     CDS             12614..14692
                     /gene="topA"
                     /locus_tag="IW819_002980"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1246048.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            14661..16037
                     /gene="trmFO"
                     /locus_tag="IW819_002981"
     CDS             14661..16037
                     /gene="trmFO"
                     /locus_tag="IW819_002981"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1246049.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            16235..17140
                     /gene="xerC"
                     /locus_tag="IW819_002982"
     CDS             16235..17140
                     /gene="xerC"
                     /locus_tag="IW819_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1246050.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            17177..17725
                     /gene="hslV"
                     /locus_tag="IW819_002983"
     CDS             17177..17725
                     /gene="hslV"
                     /locus_tag="IW819_002983"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1246051.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            17739..19139
                     /gene="hslU"
                     /locus_tag="IW819_002984"
     CDS             17739..19139
                     /gene="hslU"
                     /locus_tag="IW819_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1246052.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            19157..19948
                     /gene="codY"
                     /locus_tag="IW819_002985"
     CDS             19157..19948
                     /gene="codY"
                     /locus_tag="IW819_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1246053.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            20030..20905
                     /locus_tag="IW819_002986"
     CDS             20030..20905
                     /locus_tag="IW819_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1246054.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         7382 a         3670 c         4425 g         5562 t
ORIGIN      
        1 tatcagaaca ggatcgaatc ggtcctgttc tttttttgag aacacaaaaa gaacaaatac
       61 caaaaaaatt ttcctgaagt tcactatttt tatgatacaa tatccaagat aaggagggaa
      121 catgttgaaa gaattaaaag taactgacct caaaaaaact tatggtgaaa aagatttgtt
      181 tgaccagatt tcttttttgg tccacgaaaa agaccgtatc gggttgattg ggacgaacgg
      241 gacgggtaaa acaagtctct taaatattat tgccggtatc gatagcagcg acggtgatcg
      301 gcaaacagtg ttttatccaa ctgattaccg tattggttat ctttctcaag cagctgaatt
      361 ttccgatgaa ttaactgttc tccaagcagt ctttcaagga aacagcccct tgatccaaac
      421 tgttcgagca tatgaggaag cattgatcga actaggggaa aacggagaag accctgatgt
      481 gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa gaagatgctt ggactacaga
      541 taccaacgca aagattattt tgcaaaaatt aggaatcagt gaattagata aaaaaatcag
      601 tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga ttgatgaacc
      661 cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga tcgagtggtt
      721 ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg atcgttattt
      781 tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat acgagtataa
      841 aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag aagtagaaca
      901 agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg ctggcgtaca
      961 ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa aggagaactt
     1021 gtaccaagta aaccaagtag atgatttaga gctgaaccta gcgacacaac gtttagggaa
     1081 aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac tgctagaaca
     1141 tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa atggtgcggg
     1201 aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg gtacaatgtc
     1261 tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa tggcaccaga
     1321 taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag cagatggcag
     1381 tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta tgcatgggac
     1441 actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga aactgctgat
     1501 tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca tagcaacatt
     1561 gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg tttcccatga
     1621 ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa acggaaaaca
     1681 agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga cccagcagcc
     1741 tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga agaaattatc
     1801 ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac ttgaagaaaa
     1861 aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc gattacaaga
     1921 attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg cacggtggga
     1981 atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc gtatcttgac
     2041 ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg cacaggcaca
     2101 ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc tttgttaaca
     2161 acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt aaaaggagat
     2221 acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg ggcatttgaa
     2281 cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg ccatagatgt
     2341 ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag
     2401 atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat ctacggctat
     2461 caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa acaagtgatc
     2521 gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg gaatccggaa
     2581 gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta tgttcaagag
     2641 ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg cgttccgttc
     2701 aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg tttagcggtt
     2761 ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat cgaacaaatg
     2821 aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa tcaggaaaag
     2881 cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga cccacatccg
     2941 gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt tagctgcaat
     3001 ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc
     3061 aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga tgggacgaaa
     3121 aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg ttttgacgag
     3181 agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc
     3241 tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg tttatcaaga
     3301 atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat tcgatggaga
     3361 tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta gtcaaggaga
     3421 aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta atgagaatcc
     3481 tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga ctagctgtta
     3541 tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata aaacagagaa
     3601 gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca tatagcggat
     3661 agaattcaag ctataaaaga atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa
     3721 aataacacca gtcattccaa gagagtgata aagaggaaca gcagccacga cacagagcca
     3781 ccccataaaa atatagatga tcgtagatac ttttgatact ctctcccgtt tatgcaacgt
     3841 caacgcttta tatataatgc caagaatcgc aaacaaccag attaatacaa ataaagtcca
     3901 acctagccat cctcggatac tcagcaaaca aaaaggagta taacttcctg caatcaaaag
     3961 aaaaatcgaa ctatgatcga atacttggaa cacctttttt gcttttgtaa aaatcaatga
     4021 atgaaacaaa gtagacgtta aaaataaaag aatcatcatc gatccataga tagcataaga
     4081 gacaatatgg agggaggaat gcaagtgggc accttttact aaaagaacca ctagtcccgc
     4141 gatactcaat ccagtcccaa ttccatgggt gactgcatta aacacttcat ttaatatttc
     4201 atacgttcga ttaaatttcg ttttttccat gaaaacggct cctttactag gtgtaattaa
     4261 tgaaaaatca ttatttgtct gttatactaa aaatagaaat tgtcttacat gtagtttaac
     4321 atgaataagg catcttgtta aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg
     4381 taacagattc ttcatgtacg atggaaaaaa atgtacgcga tgctttaggc attcgtgtca
     4441 tgccgctatc catcatgatc gacggaacag tatatcccga tgatgaccaa ctagacggcg
     4501 aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc caaaacaagc caaccaccaa
     4561 ttggagaatt catctcgatg tatgatgagc taggagaaga cggcagtgaa gtgatttcga
     4621 tccatatgac aaaaggcttg agtggaacag tagaagcagc aagacaagcc agtaatctaa
     4681 caaaaacgaa agtaacggtt atcgacagtg ataccactga ccaaggatta tcttttcagg
     4741 taattcgagc agctgaatta gcaaaagaag gtaaaacagt agagaaaatc cttgaagaag
     4801 tcaaaaaagt acaagacaat acaaaattat acatcggaat atccacacta gataacttgg
     4861 taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc tagtgttttg aacatacgcg
     4921 tggtgatgaa tttcgcccat tctgaattaa tccctgtaac taaggggcga ggaaagaaaa
     4981 cgttcgataa atggtttaat aatttgaaaa atgaactgaa agaattgcca aatgtgcgac
     5041 aaatcggtat ttcacatgcg gatgcaaaag aattggcgca agaattcaaa gagggtttgc
     5101 aagaattatt cccggacatg catatccctg tactgcatac gaatccggtt atcgctacgc
     5161 atactggtaa agacgcgttt gcaatcatgt attatgtaga ttagtcgaaa agaggataag
     5221 ccctcagcct gaaaggcttg cagcttattc tctttttctt tataaaatta ggaaaataat
     5281 tgttttcttt tttcataaag actacctttt tgataaaatt tttgataaca ttagaaccaa
     5341 tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac atttgatcct cgttttgttc
     5401 ccattattaa ttgcgatcat catctatgca ggtttagctg cttctatacc gaaagctggc
     5461 ccactaatga aaccagtaac tactgtgaga gaagatccaa ataaaaaaga aaaacttcac
     5521 tacatagcta tcggagattc gctgactgaa ggagtaggag atcaaaccaa gcaaggagga
     5581 ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata atttgaccgc ggttgaaaca
     5641 gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct taaagcgagt aaaaacaaaa
     5701 gaagaaattc gaaacaatat caaaacagct gattttatca cactgactgt tggcggtaat
     5761 gatttgatga aagttatcca aaataattta ttcgggttaa cggtcaattc ctttaaaaag
     5821 ccactgaaaa actatcagga aaatgtcaca gaactattgg aagaatttcg atccctcaat
     5881 aaaaccgcac ctatttacat tttaggcatc tacaatccat tttatttgaa ctttcctgag
     5941 atcactgaca tgcagacaat tgttaacaat tggaacaaag gaacagaaac ggtgacaaaa
     6001 aaaatgccga actgctactt tatcccaatc aatgatttgc tttatcaagg attgaatcag
     6061 gaagtaggtg tcacagaaga aggaacagat caaacaacta gcacgacaga aaatctaaat
     6121 ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc atccaaataa tttaggttat
     6181 caattgatgg caaatgcagt gagagatcag ctgatcgaga cacaaaacca atggttagag
     6241 aaggaggatg atcaataaac atgaaaacaa gatctgacgt atcttcaaac aaaacaaaga
     6301 aaacaaatta ctggaaattc gccttcctta tccttcttgg ggtagtactt ggttcgaccg
     6361 tatttttagg cagccgtatt tttgctaatc gggaaccaga actcccagaa ataccagctc
     6421 ttacggaaag acaaggagat cctgtcttaa cgatcaattc aaataaagaa aaagtcaatc
     6481 aaatcatcag ctttttcctt agtgagtatc aaaaagatag tgatattgaa tataaatttt
     6541 atttggaaaa tgaagcacta ctcaatggta ctttcgaagt actaggtttt cctatcgatt
     6601 tctatttata ttttgatccg tatgtgatgg aaaacggaaa tgtccaacta aaagctagaa
     6661 gtctttctat cggaacattg agtttgccaa tcaaagatgt gatgaatatg atcaagagaa
     6721 actataaatt accagattgg atcgaagtca atacagatga tctgactatc atgcttaggt
     6781 tggatcaatt ccgaatgcaa aacgggatgt atattaaagc agataaaatc gacttagtaa
     6841 atgatgacat tcggtttagt ctatatttgc caaaagatac ctctgatgct tcaaacaact
     6901 caaataccaa caaaactagc accagcagta ctgaaagcaa gacaaaagaa aataaaaaat
     6961 catcggatga gtagtattag agggggaatc ataatggaac gagcaatatt tgcaggaggc
     7021 tgcttctggt gtatgatcca gcctttcgac acgcttcctg gtatccatac agtgatgtca
     7081 ggatatactg gagggcatgt accagatccg acttatgaac aagtaaaaag ccaaacgact
     7141 ggacatacag aagcagtgga gattatctat gatccaacat tgatttcata tgaacaatta
     7201 gtagaacttt actggcagca aactgatcca actgacgctt tcggacaatt cgaggatcgc
     7261 ggtgacaatt atcgcccggt gatcttttat ttgacagaag aacagcggat cattgctgaa
     7321 aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac agccaatcgt tacaaaaatc
     7381 gaaccagccc aaccatttta tctagcagaa gaagaccatc aagagtatta taaaaagcat
     7441 cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct ttatcgaaga aaattggagg
     7501 aacgaagggt gaatcgaagc ttttttcatt atttaatgac actgcgaggc ggtggcacac
     7561 atgatgcttt atctgttttc gctacagaag ccggtaaaga catccaattt cctaagcaca
     7621 gctctagcta cgaagaaatt tctaattatc tggaactgaa tgtcgactat ttgccgtcca
     7681 tggatatctt tgacgaagca tgggaaaaat atttagaaaa taatcatgac atataagaga
     7741 tagttttgta ctttatgatc aaaaaagttt tttgtccaat agcctaattg aaagaattat
     7801 ttttatttca gctatctagt cgcatttttt tgatattttt cttataataa agaaagaatg
     7861 aaaaaaagag ggatggattg atagagattg acaaaaaaac aacgctatat taatcgcttc
     7921 tggcttgtat tcaaatactt acttgtttct gtattccttg cgttcatgtt gcggggattt
     7981 ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa acgtgttgaa gcaaggtgat
     8041 atggttgtga tggaaaaatt ttcggagatc agacgatttg atattgttgt ttttcaatta
     8101 gcagatggca cgatttatat caaacgagta atcggtcttc caggagaaaa tgtcagctac
     8161 caaaatgatc aattgaagat caatgggaaa gtagttaagg aaccttatct aactaaaaac
     8221 ctgaaatcag atcatgcaaa tgcatcctat acgacggact ttacactaca agagctaacg
     8281 ggacaatcca agcttccgga agataattat tttgtacttg gagataatcg aagagtttca
     8341 aaagatagtc gttcgtttgg cacgatcaat aaaactgata ttctaggtaa agcaagattt
     8401 gtctattatc cacttgatga aataaaatgg atccagtgag aaacaataaa gcgtgcagtc
     8461 tgttgagact gcacgcttta ttgtttaatc caaaatcagt ttactacctt agtacgtaaa
     8521 atcaaacagc tcgtttaaac acttttctag cctctaaagt tttgataaat gaataaacaa
     8581 ccattatttg aatagggatt tggatcatat tcctgatgaa tctacctaga aaaattactt
     8641 tgaaatctgc accaaacaaa acagacatca gccaagaatt gataaaaagg ctgactggga
     8701 tcataatcaa taattgggca gcgatgacgg aaggcaggtt tatcttttta tgatataaaa
     8761 ataagccatt aataaatcca ccaaggaaag ccgtgataag aaaagcagct tgaaacgttc
     8821 ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat ttcaagcaaa ccagcccaga
     8881 tcggtccata tagataaccc ataagcgcca tacttaaaaa tccgaatgtg atttgaaaat
     8941 aagcaggacc aatcgctatc ttgtataaga tgacatcaag ggctaataaa agagcagagg
     9001 tcgacaacaa tcttgcattc atttcgggca atctaaaaac gtcttttgca atcaaagtgt
     9061 tcaaaatatt ctctcctttg tcagttaata ataaaaggat accataaaat caccaatgtg
     9121 tcatgacaca ttggtgattt acatacactg gcatctaaca attaaaaaaa gggtgagaag
     9181 actgctttta taaactgtaa aaaagagatt gtacgaataa aaaaaataga ggcggagaat
     9241 acttatcgta tcatccgcct ttgttttttt gttataaaaa acgtcagatt agttatctgc
     9301 ttacatcttc atccatacgg gttctaatgt atttccgtct agatcttgga cttctaatga
     9361 aaacatttct tcttcaggca ttcccatatc tacttcgtgg aaagtaccac cattttcttt
     9421 tgcaatttga gcaaattttt ttacttcatc cgcattttca acactaaacg aaatcaaagc
     9481 accacttgtt tttttattat cagctatttc tttatctttt ataaattttt tataaaaatc
     9541 atgagagaga agcattatcc aaaaagtgtc atcccatacc attgaaccag cttcttcagt
     9601 agaaaattct tcattttttt taaaacccag cttttcataa aaagcaacgg aacgattgac
     9661 atcagcaaca ggaaaattaa caaaaaccat actattcatt tgtttcgtct cctttctgtt
     9721 ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa aatttagatt catatataga
     9781 aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg tgatagaatg aggactggtt
     9841 cactttcact taactaatag atggaatttc tttataataa aaaagtgaat ttatattgaa
     9901 aaagaaaaaa acaagtaaag ccggggaata ttccggtatg aaaggaatgg agagaacaat
     9961 gaccatccaa tggtttccgg gacatatggc aaaagcccga cgagaagttt ctgaaaaaat
    10021 gaaatatgtg gacatcgtat ttgaattggt agacgcacga ttacctttat cttctcgtaa
    10081 tccaatgcta gatcagatca ttcagcaaaa acctcgcctt gttttattga ataaagcgga
    10141 tctggcagat ccacaacaga caaagctgtg gcaagaatat tttcgtaaac aaggccatct
    10201 tgcattagct atcacggcac aagaaagtaa aggaattaaa ggtctagtac ctgcggctaa
    10261 agaagcattg aaagaaaaac gggcaagaga tgctgctcgg gggatcaagc ctcgcgctat
    10321 tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct acgttgatga atcgactagt
    10381 aggcaagaaa attgcacaga caggaaataa gcctggcgta acaaaaggtc agcagtggct
    10441 tcgtttaggt aatgagttgg aattgttaga cacaccaggg attttatggc ccaaatttga
    10501 agatgaagaa atcggcaaaa aactagcgct gacaggcgcg atcaaagacc agcttgtcca
    10561 tttagatgat ttagcgattt acggattaga cttttttgcc cgatattatt caggaagaat
    10621 cagcgatcgt tatggtttat caatcgagca agaagaaaaa ttggcaccgg aattactgat
    10681 ggatattaca gcaaaacgtg ggtataaaga agactatgat cgtggcagcg aattagtcgt
    10741 gcatgatatt cgtcaaggaa aacttggacg atacacactg gatcgttgcg aagaaatgga
    10801 gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga acgattgaaa agcatacaga
    10861 gaatagatga tcctacacta gaatcattaa gaaatgatcc acgcaaaggc gtgcagcaag
    10921 cactggcttc ttttgaacga cgattgaaaa aggaagcatt aattaaggaa aaatatgaag
    10981 aaatgttttg ctttgaaaag caagggtacg cacaaggtca ccaatggatt gccggcattg
    11041 atgaagtagg aagaggacca ttagctggac ctgtcgtggc agctgcagtt atcttgccga
    11101 aaaatgagat catattaggg ctgaatgact ccaaacaatt atcagagaaa aagcgagaac
    11161 ttctttatca acaagttcaa gaaaaagccc tggcaatcgg gatcgggatc gttgatcagc
    11221 agacaatcga tgatgtgaat atttatgaag caagtaaatt agcgatgatc caagcagtag
    11281 aaaaattgtc cattgtacca agctatttgt tgatcgatgc gatgaccttg gatttgccta
    11341 ttcagcagga aaaaataatc aaaggggatg cacgatctgt gtcgattgct gctgcaagta
    11401 tcattgctaa agtatatcga gaccaattga tgaaagaata tgacgtgctt tatcctggat
    11461 atggtttctc gcataatgca ggttatggaa caaaggaaca cttagaagga cttgaacgac
    11521 aaggaattac gccaatccat cgtaaaagtt ttgcacctgt aaaaaaatat ttacaataaa
    11581 aaacagccgc ttttacgtat cttattagaa agagagacga aaggcggttt tatgatgtat
    11641 caaatagaag aaaatttatt gaaacttgct tatacaaaag gaatcagtag tcaaggaaaa
    11701 tgggcacttg ctaactggat gatgcagtat ccttataaag aaatcggatt tgaagacatc
    11761 attaagatag gtaaaataac caaacaccgg gagctgtttc gcaaaagttg gcaggaaatt
    11821 cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta tcacttgctt tgatcccaaa
    11881 tatccccctc agctgcttca cttgacctat ccgccaattg tactgtttta taatggtgat
    11941 ctatctttac tttcttgtaa catgttaagc gttgttggtg gaagacacac ttctgcttta
    12001 gccaaaaaaa cagtttatta tttactaaaa ccagtgatag aagctggata tgtgatcgtc
    12061 agtggctgcg cgaaagggat agatacatac ggtcaccaag cagcaatctc tcatggtgga
    12121 cggacaatcg cagtcattgg aacagggata gagcaagtct atcctgctga aaatcgtgac
    12181 ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg aatatcctcc tcaatccggc
    12241 ccgaaaaagc attgttttcc aatgagaaat cggattatcg cagctttgtc taaaggaaca
    12301 tgtgtctttg aagccaagaa aaaaagcggc tctctgataa cagcacagca agcattggat
    12361 cttggaagaa cagttttttc tgttccagga aatattttaa cagaacattc taccggatgt
    12421 catcatttga tccaagacgg tgcagtttgc acggtttcgg gtgaagatat cttggcagaa
    12481 tttaaagaat aacaccatac ttttcatttg cttttcttga caaacgtttt ctgaatggat
    12541 atgattagct acgatttgta tgaatcctct tttaatagta ggaacacgac aatggaaagg
    12601 agtccattca gtaatggcgt ataaatattt agtgattgta gaatcacctg caaaagccaa
    12661 gacaattgag aaatatcttg gacgaaacta taaagtaatg gccagtgttg gccatataag
    12721 agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac aattatgaac cccattatat
    12781 ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa gccgcagcaa aaaaagctca
    12841 aaaagtttac ttggcaagtg acccggatag agaaggagaa gcgattgctt ggcatttagc
    12901 gtacctactt gggttggatc tgaaagaaaa aaatcgggtg gtcttcaatg aaatcacaaa
    12961 agacgcagtc aaagcagctt ttaaggaacc aagaacgatc gatgtagatt tagtagatgc
    13021 acagcaagct cgtcgtacct tagacagaat cgttggttat tcgatcagtc ctattctctg
    13081 gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa tctgtcgctt taaaaattat
    13141 tattgaccgt gaaaaagaga tccgagaatt tgttccagaa gaatattgga gcatcgacgg
    13201 taattttaaa aaagctcgca agaaattcaa agcaaatttc tggggaatcg acggtaagaa
    13261 aaagaaatta ccagatgcac aaagtgtaaa agaagtcact gctagaattg atggaacaga
    13321 cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga aaccctgcga atcctttcac
    13381 tacaagtagc ctgcaacaag aagctgcccg taagttaaac tttcgaacaa gaaaaaccat
    13441 gatggtagca cagcaattat atgaaggaat cactttgggt aaacaaggaa cagtcggctt
    13501 gattacatac atgcgtactg attctacccg gattgccgat acagcaaaag cagaggccgc
    13561 agcatttatt gaagatacgt atggaaaaga attttctaga catgatcacc gaaagatcaa
    13621 aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt ccaacgagtg tgcttcgtac
    13681 accagatgaa ctcaaacaat atttagataa agatcaattg aaactttata cattgatctg
    13741 gtcacgtttt gtggctagtc agatgacacc agctatttta gatacaatga aagtgacatt
    13801 agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa atcaaattca aaggattcat
    13861 gcaagtgtat gtggaaggta gagatgacgg caaagaagag aaagaaaata ttcttccaga
    13921 acttgaagaa ggcgatgtag tccaatctgt tgatattgaa ccaaaacaac attttactca
    13981 accaccagca cgttttagtg aggctacgtt gattcgtgca ctagaagaaa acggggtagg
    14041 gcgaccttcc acttatgcac caacacttga tacgatccaa agacgctact atgtaaaatt
    14101 gacacaaaaa agatttgagc cgactgaact aggagaaatt gtcaattcat taatctgtga
    14161 attcttccct caaatcgtcg atatccattt cacagcagaa atggaaggag atttggataa
    14221 aattgaagaa ggcacggaag cgtgggtcaa agtggttgat cgtttctata aaccgtttga
    14281 aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt caaatcaaag atgaaccagc
    14341 aggattcgat tgtgatgtct gtggacatcc gatggtgata aaattaggga aatatgggaa
    14401 gttttatgct tgcagtaatt ttcctgattg tcggaatacg aaaccaatcg taaaagaaat
    14461 cggtgtgact tgcccagtat gtcatgaagg acaagtcatt gaacgaaaat ctaagaaaaa
    14521 cagactattc tatggttgtt cccgtttccc agaatgtgaa ttcacttctt gggataaacc
    14581 agtgggcaga aattgtccaa aatgtgacgg atatctggtc gagaagaaag tcaaaggcgg
    14641 taaacaagta gtctgtgtaa atggagatta cgaagaagac atccaaaaat aatagaaaag
    14701 aggttctttc attgaaaatg gtaaatgttg ttggagcagg gcttgccgga agtgaagctg
    14761 cttggcagat cgcacaagca ggagtccctg tccgtttata tgaaatgcgt ccagtcaaat
    14821 cgactgaggc gcatcacaca agtaattttg ctgaactagt atgttccaat tcattaagag
    14881 gaaacagttt ggctaatgca gtagggttat tgaaagaaga aatgcgccga ttgaattcgg
    14941 tagtaatcac ttcggcagat gaaacagcag tgccagctgg tggtgcctta gctgttgacc
    15001 gcgattcttt ttctgaaaca attacccgaa aagtcaaaga gcatccatta gtaacagtga
    15061 taaacgaaga aattactgaa ataccggaag gaatcaccgt agtagcaaca ggacctttga
    15121 cttctcatcc attagcagaa agcatcaaag cattcaatgg ttcagatggt ttttatttct
    15181 atgatgcagc tgcaccaatc gtagacaaag ctacgatcga tatggataaa gtatatttga
    15241 agtcccgcta tgacaaagga gaagcagctt acttgaactg tccgatgaca gaagaggaat
    15301 tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc agaacttaaa tcttttgaga
    15361 aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat ggcacagcga ggattcaaaa
    15421 cgatgctttt cggacctatg aaaccagttg gattggaaga tcctaaaact ggcaaacgtc
    15481 cttatgctgt tatccagctg cgtcaagaca atgctgcagc ttctttatac aacatcgtgg
    15541 gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt tttccggatg attcctgggt
    15601 tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg aaacagcttt atgaattcgc
    15661 cagaattgct gaaaccgact taccaatcaa cgaaacgcga agatctgttc tttgcaggtc
    15721 agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag cggactactt gctggaatca
    15781 acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt tccacaagaa acagctattg
    15841 gaagtatggc ttactacatt actcatgcgg aaggaaaaca tttccaaccg atgaatgcca
    15901 actatggtct gtttcctgaa ctgccagagc gcattcgaga caaaaaagca agatatgaag
    15961 caattgcaca acgagcttta aaagctaatg aggcaataaa aacagaatta gaaaaaactg
    16021 caatcccaaa tgagtaagga aactccatga agagggtgag acggatagtc tcgctctttt
    16081 tcttgcgttt ttcttaagaa tttctcaaac aaaatgtgag gaattgcgcc aattgtgaac
    16141 aaacttagaa atattggtta aattctgaca atttatttgt ctttttataa acgctgtgct
    16201 aaattattct ctgggacaaa aggaaggtga gaaaatgaca gaagccacgt gggaacaaaa
    16261 atttttgcgt tatttgattg tcgaaagagg atattcagaa aaaacgaaag aagcatatga
    16321 agaagacatt ctccatttca aacatttttt ggaggaatct ggagacgctg atctgctgaa
    16381 ggcggaatat tttacggttc gtgtctattt gagtgcatta gctgatcatc gtcctagcta
    16441 tagcagaaat tctatcagtc gcaagatagc cagcctacgt tcgttttatc agtatcttct
    16501 caaagaagaa gtgataaaag aaaatccatt ttcttacgtt catctcaaaa agaaaaattt
    16561 gagactgcca cgtttttttt atgaaaatga aatgcaagca ctttttgaca gcgtagcagg
    16621 agatagtcct ttggatttga ggaaccgcgc tttactggaa gtcctttacg ggtcaggcat
    16681 ccgtttaagt gagtgtagca gtttgtcggt atcagacatt gattttgatt cagaggtcat
    16741 gttgatccat ggtaaaggga acaaagagag atatgctcct ctaggttctt ttgctcaaga
    16801 tgctctgcaa gaatatttca gggaaggccg ccaggtattg atgacaaaat atcacgaaga
    16861 gcatgatatc gtttttgtga atcatcatgg aaaaccaatc acgcctactg gaattgaata
    16921 tgtcttgaat caaatgatca aaaaaagcag tttggatagt gatatccatc ctcacatgct
    16981 gcgccataca tttgctactc atttattaaa taatggggca gatttgcgga cagtccaaga
    17041 attgctagga catgccaatt tatcgactac ccagatctat gcccatgtaa caaaagaaag
    17101 tttacaaaaa aactatcgtt ctttccatcc aagagcgtaa aaataaggaa aagaacaaat
    17161 ttaggaggaa acaaacatgg tcgaatcacc atttcattca accactatct gtgccgtcga
    17221 aaaagacggg aaatttgcta tggctggaga tggccaagta acaatgggtg aatctgttgt
    17281 catgaaaggc actgcaaaga aagtacgacg tatctacaat ggagaagttg ttgtaggttt
    17341 cgccggaagt gttgcagatg cgtttacttt agaagaaaaa tttgaaggca aactaaacga
    17401 atataacggg aatctacaac gcgcagctgt cgaattagct caagaatggc ggacacagca
    17461 atctatgcaa aaacttgaag cgatgctgat cgtaatgaat gataaagaaa tgcttcttgt
    17521 ttcaggtaca ggagaagtta tcacaccaga tgatggtatc ttagctatcg gatcaggagg
    17581 gaattttgcg ttggctgcag cccgagcaat gaaaaactat gctcaaaacg acatgagtgc
    17641 gaaagaaatt gcagaaaatg cgttaaatat tgccgcagat atctgtatct ttactaatca
    17701 taatattatc gtagaagaac tgtaagaggt gagtagatat gtatgaatta aaacaaacac
    17761 caagacaaat cgtaagtgaa ttagatgaat atatcgtagg acagcacgca gcaaaaaaat
    17821 ctgttgccgt tgctttgcgt aaccgctatc gccgtttgca gttagatgaa aaaatgcaac
    17881 aagatattac accaaaaaat atgctgatga tcggacctac aggggtcggt aaaacagaaa
    17941 tcgcacgacg tttggcaaaa atcgtcaatg caccatttgt caaagtcgaa gcaacaaaat
    18001 ttactgaagt cggttatgtc ggaagagatg tagaatcaat ggttcgggat ttagtagaaa
    18061 atgcgatcca aatcgtagaa aaagagcagt acagccgtgt atacacaagt gctaaaaaaa
    18121 atgcagaaaa acgtttggta aaaatcttgg tacctggtat caaaaaagaa cagaaacaat
    18181 ccactaatca atttgaacaa atgatgaatc tttttaatgc tagtcagcaa aataccgctg
    18241 aaccgcaaga agaagtgaca gaagatattc gaatcaatcg ccgtacagtc ttggaacaac
    18301 tacaaaaggg tttactggat agtcgtgaag taaccattga agttgaagac caaaagaaac
    18361 ctgcaccaat gatgaacaat ggattggagc aaatggggat cgacctaaat gaaactctag
    18421 gtgctttgac accgaagaaa aaagtggaac ggacggtaac agtcaaagaa gctttagagt
    18481 tattgatcaa ggaagaatca gcaaaattag tcaaggatgc tgatatccat agtgaggcaa
    18541 tcaaattagc ggaagaaagt ggaattattt ttatcgatga aatcgataag atcacttcta
    18601 aatcacagca atctggagaa gtatccagag aaggcgtcca aagagacata ttgccaattg
    18661 tcgaaggttc acaagtaaac acaaaatacg ggatcatcca aacagatcac gtcctattca
    18721 ttgcttcagg agctttccat ttgtctaaac caagtgatct aattccagaa cttcaaggac
    18781 gcttcccgat acgggtagaa ttagatgatt tgacagctga agattttgtg aagattctga
    18841 ccgaaccaaa taatgcttta gtaaaacaat acgtcgcgtt gatcggtaca gaaaatgtgt
    18901 cagttacgtt tacaaaagaa gcaattgaac ggatcgctca aatcgctttc gatgtcaacc
    18961 gtgacacaga taatatcggc gcacgcagac tgcataccat tttggaaaaa ctactagaag
    19021 atctattatt tgaagcacca gatatgcaaa tgggagaaat aacgatcaca gaagcatatg
    19081 tggatgacaa acttggctca attgcggcaa atgaagattt aagtcgctat attttataaa
    19141 aaatcggagg acgacaatga cagacttgct aacgaaaacc cgcaagataa acacactttt
    19201 gcagcaaaag aatacttttg acatgcaagc agatttgcct tacgataaaa tggctgtcac
    19261 actaggtggt attcttgaca gcaacaccta tatcatagga agtgacggag tattactagg
    19321 ttataatgaa cgtcacgatg tgaacaatga acgaatcaaa acaatgtttg ttgagaaaca
    19381 atttcctgaa tcttatactg atacagtggc acagttgatg aaaactgaag ctaatatccc
    19441 aattactagt gatattactg catttccggt agagtcaaga gagaaatatc catttggtct
    19501 gacgactgtc gttccgatct ttggagctgg cgaacgtttg ggtacgatta tcttatctcg
    19561 tatggaacaa tcgtttgcag atgacgatct agtcttagca gaatatggag ctacggttgt
    19621 cggtatgcag atcctttatc agaagtctcg aagcatcgaa gcagatgtac ggagtgcaac
    19681 ggctgtccaa atggcaatca acacattatc ttatagcgaa ttgaaggctg tacaagctat
    19741 ctttgaagca ttggatggag atgaaggtcg tctgactgct tctaatattg ccgatagtat
    19801 cggcatcact cgttctgtga tcgtcaatgc tttacgaaaa ttggaatcag ctggaatcat
    19861 tgaatctcgt tcgttaggca tgaagggaac ttatttaaaa gtattgaatt cccgttttaa
    19921 agacgagtta gcaaaacaca gttattagaa gtaaggggac tggggcagat agaaatgttt
    19981 gttccagcct tcccatttta aaaagtaagt cgaagttgga ggaatggcaa tgactgttat
    20041 tattcaaaat gaccagctga tcgccgaaat cgctgaacac ggtgcggaat tgatcagtct
    20101 gaaatcaaaa gaacatggtt tagagtatat ctggcaaggt gatccagcgt attggggcag
    20161 acatgcaccg gttttattcc cttttgttgg acgtttaaaa gatgatcagt atgtttatca
    20221 agggaaaaca tatcaaatgg ggcaacacgg atttgctcgg gatatggatt ttgaagtcat
    20281 tgaacatgga caagaaatgg ctagtttcat cttaaggagt acgccggaga cattagaaaa
    20341 gtatccattt gattttgaat tagtgatcag ctatgaatta ggaggagacg gcatcacggt
    20401 tcattatcaa gtggcaaata ccggtgaaaa agaaatgttc ttttctatcg gtggacatcc
    20461 tgctttcaat gtacctcttg aagaaggatt gtcatttgaa gatttctatc tggcattttc
    20521 tccattgaaa tcaagattac gcctaccatt gaaaggccca ttcatcgata tggatcaaaa
    20581 gacgttaggt cagacaaata cgaatctggc tttgaatcgt gaactttttg agcaagatgc
    20641 cttggttttt gaaacaaggg gtctaaatgc gtacgccatt cgaagtgaaa aatcttccca
    20701 tagcgtaact ttaagctata aagatatgcc ttatgtaggg atctggtctc cttatccaaa
    20761 agaagcgcct ttcgtttgta tcgaaccgtg gtgcggaatc gctgacacgg tgaacagtag
    20821 tggaaacttg atggaaaaag tagggatcaa tcaactaggt gcccatgaaa tcttcaagac
    20881 aaaatattcc atcacagtga agtaagaggt tgtgaccgag aagttagcag ggaacaccgt
    20941 ttaccaaggt gtgtaaaaaa gctatcctgt aagtaataag ggagtgtgac atcgatttgt
    21001 ggctctttgt caataaggac tgatgagttg tgcaaaatc
//
LOCUS       DACEPW010000430         8062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.guided.116, whole genome shotgun sequence.
ACCESSION   DACEPW010000430 DACEPW010000000
VERSION     DACEPW010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.guided.116"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1564
                     /locus_tag="IW819_002987"
     CDS             128..1564
                     /locus_tag="IW819_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1246055.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IW819_002988"
     CDS             1753..4200
                     /locus_tag="IW819_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1246056.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IW819_002989"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IW819_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1246057.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IW819_002990"
     CDS             complement(5690..6793)
                     /locus_tag="IW819_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1246058.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW819_002991"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW819_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1246059.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DACEPW010000431         5918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.guided.141, whole genome shotgun sequence.
ACCESSION   DACEPW010000431 DACEPW010000000
VERSION     DACEPW010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.guided.141"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            405..869
                     /locus_tag="IW819_002992"
     CDS             405..869
                     /locus_tag="IW819_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1246060.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            853..1482
                     /locus_tag="IW819_002993"
     CDS             853..1482
                     /locus_tag="IW819_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1246061.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1836..3314
                     /gene="msr"
                     /locus_tag="IW819_002994"
     CDS             1836..3314
                     /gene="msr"
                     /locus_tag="IW819_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1246062.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3361..3807
                     /locus_tag="IW819_002995"
     CDS             3361..3807
                     /locus_tag="IW819_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1246063.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(4006..4521)
                     /locus_tag="IW819_002996"
     CDS             complement(4006..4521)
                     /locus_tag="IW819_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1246064.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4638..5839
                     /locus_tag="IW819_002997"
                     /pseudo
     CDS             4638..5839
                     /locus_tag="IW819_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1831 a          992 c         1245 g         1850 t
ORIGIN      
        1 gaacttatta ccgcagatgc aagtttttga acaccgttta ctcaaaaaag gttgtgacaa
       61 catcttgtca taactttttt attactgttt aagcatactg atccaatttc taatattcga
      121 cgggattctt gcaaaaggcg atttcggaaa tttttggcgg tgtaaaattt tctactgcag
      181 aagagctatc ttctatagtt tgataagaat caataatctt ggttttagcg ttgtgctttc
      241 tttatcattt tttccgttca ttctacaaaa aatttctttt ctctatactt ttctattgag
      301 agcgtttttt ttattacaca gctgattctt tgactttttt ctttttttat tctattattg
      361 acaagtcaat cgttgacgag tcaattatta gaaaggagga aattatgtct ttagaagaga
      421 ttagtcacct tttatatcaa atcaaattag ccgatcaaaa aatcgtccag atctttgaag
      481 aaaatatcgg tataagtctt actagatacg agatcatgat gagactgaaa gaagaaaatc
      541 cattgctaca aagcagtttg caagaaaagt tgcagattga tcaaggagca gttactcgtc
      601 atctgaaggt attggaagca aatggctatg tttcgcgtca gagaaatcct cagaacaacc
      661 gggaagtgta tgtagcgcta acagaaaaag cccagaagaa aatcgagcat tgtgaaatgg
      721 accataaaga tacaggttcc gtgctatccc cggctttcag cgatgtagaa atgaaacaat
      781 ttctaacact tttagataaa ttgaataccc aactttgcaa tataacaaca gaaacgaaag
      841 aggttaataa taatggaaca aaaaaatgat ttttcaacaa ttatgactgg tagaagatcc
      901 gttcgcgtat atgatgaaac atacaaaatc ccacacgaag aaatgctgga aatgatcaaa
      961 gaagcgacga ctgcaccttc ttctgtcaat atgcaaccat ggcgctttgt ggtcgctgaa
     1021 agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat
     1081 acttcttctg caatggtcat gatttttggc gatatgcaat gttacgaata tggcgaagaa
     1141 atctataacc aagcttacga atcagggaag atgccaaaag aagtcagaga tcaacaatta
     1201 gctgcgatca ttccttatta caagagtttt tcaagagaag aaatgaacga tgtagtcaaa
     1261 gtcgattcta gcttagcggc tatgcaattc atgttagttg cgcgtgatca tggctatgaa
     1321 acaaatccaa ttggtggatt cgaagcagat cagttggcag aggcatttgg tttggataaa
     1381 gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga aggttacgag
     1441 tctgttcgtt tagcaccaga aaagatcact acgttcgaat aaaaggtttt cagttactat
     1501 tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa
     1561 aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa ataaattcaa
     1621 aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg aacgacagta
     1681 gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg ttcagagtag
     1741 gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt agtatcgata
     1801 attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa
     1861 caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct
     1921 atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca
     1981 aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca
     2041 actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca
     2101 gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac
     2161 gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc
     2221 caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt
     2281 atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa
     2341 tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca
     2401 ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga
     2461 aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag
     2521 tgtccagtaa aaagaaaaag caacaaatca gaccggatcg gttgtcttcc tctaaacaaa
     2581 aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa
     2641 aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat
     2701 ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg
     2761 ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg
     2821 tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag
     2881 gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga
     2941 catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg
     3001 tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta
     3061 cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta
     3121 atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag
     3181 agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg
     3241 tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta
     3301 cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga
     3361 ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc
     3421 ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg
     3481 attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg
     3541 ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt ccaaatgatc
     3601 ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt attcggtgtg
     3661 atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc ttggaaaatg
     3721 aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc aagaagtgtg
     3781 ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa ggccttggca
     3841 tgctttttgt catagtctct tgaatgcagt tctagaaaaa tagtgatccg aacgataggt
     3901 tttggcctgt cgttcggatc gctttttctt ttccaactgt attgctcttt tattgaagat
     3961 aatttgctct tcaatagata tttctagaaa ggttctgttt atcccttact caaaaaactg
     4021 ctgcatttgt tctcttactt gctgacacca ttggagttcc tgctgacaaa gcataattct
     4081 gcgctggatc acaacataac gtccaaattc ttgtttcttg tctagcgaga ggtcattcaa
     4141 ttttttttga tttttttgca tgattttgtt cagctgttgt tctctagtga atagcagtcc
     4201 agtagctgtt gttttatcga gcgtcgaaaa agcatagatt tttgctagaa attcatcttt
     4261 ttgcgtagga gcgggagttt cctctttgat ccattcctct acaagagcac gtccttcatc
     4321 agtcaagtgg tagatctttt tttgagagtg ctcgtctttg tccaaaacgg tcacataacc
     4381 ctgctggtct aacttggcaa gcgttgtata gatctgactg tgatgggctt cccaaaaaat
     4441 tgtgatcagc tgcttcaatt catacccaga acgtggtttg gataaaagca cgcataataa
     4501 tatataggct aaagtattca taaaaatcct acctctcgtc ttattttctt tattatatca
     4561 cagtaaagtt tccacgtttt acatgtgcat atttacatgt aaaataaatc atgtatgatg
     4621 ctaatagaga gaaagggatg gaaatattgg agaatcaaat agatatacaa gaacaaacaa
     4681 atgaagaaac aaaggggaaa agttcagcgc tagttgtagc agctacctgt atgatcgctt
     4741 ttatgggagt gggtctagta gacccaatct taaaaacgat tgctgcacag ttaaacgcta
     4801 cgccagctga aacgaccctg ttatttacta gttacatgtt agtcactggt gtggtgatgc
     4861 tatttacagg tttcttatct agtcggatcg gtttgaaaaa acattgatga caggattgtt
     4921 tatcatcgtt gtattcgctg gtcttggcgg attatctggg aatatcggaa tgctgattgg
     4981 gttacgtgcc ggctggggtg tcgggaatgc tttatttgtc tctactgctt tagcaacgat
     5041 cgtcagtatt ttagttggta acacagaaaa agcaatcatg atgtatgaag cagcacttgg
     5101 ctgcggaatg gctgttggcc ctctagtcgg tggggtcttg ggcagtggtt catggcgtta
     5161 tccatttttt ggggtagcat ttttgatgtt ttgcggtttt atagccctag cggtactttt
     5221 acctgaaact gagaaaccaa agagaaaagt aggcctttgg gaaggtgtca aagcgttaag
     5281 caatcataaa cttcgttcag ttggactaat cgcattgtta tataatttcg gattctttac
     5341 tttacttgcg tatgcgccat ttttattgat tggctattct gaattagaag tcggttttgt
     5401 attttttggc tggggtgtgc ttctggctat tgcttcgatt ttcgttgcac cggctcttga
     5461 gcggaagttt acgacttatc ggacaatttt agcagcattt gtcttattca ttatttgttt
     5521 gctgttatta ggagctggaa cttctatgcc agtccttgta ccgatcagca ttgtgttagc
     5581 aggatttttc caaggaatta tcaatacact gttgacgacg attgctatgg aaataccagg
     5641 tctgcaacga aacgttgctt cgtccagcta tagttttgtc cgctttttcg gtggagcttt
     5701 agcaccattt attgcaggtg tgattggtga acatattggt gaaagctatg cgttttattt
     5761 tgcaggtgcc attgttctta tcagtgtatg ttgtatcatt tatcaccgta attattttgc
     5821 tacaaatgaa gaagaataag aaatcagagg ttgtgaaaaa gctgtcctgc atcaagtcat
     5881 aagaacagcc aaacaaaaat agcttctcat atttttcg
//
LOCUS       DACEPW010000432         4995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.guided.164, whole genome shotgun sequence.
ACCESSION   DACEPW010000432 DACEPW010000000
VERSION     DACEPW010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.guided.164"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(30..2180)
                     /locus_tag="IW819_002998"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="IW819_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="IW819_002999"
     CDS             2326..2901
                     /locus_tag="IW819_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1246065.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="IW819_003000"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="IW819_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1246066.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="IW819_003001"
     CDS             3788..4942
                     /locus_tag="IW819_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1246067.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DACEPW010000433         4717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.guided.174, whole genome shotgun sequence.
ACCESSION   DACEPW010000433 DACEPW010000000
VERSION     DACEPW010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.guided.174"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            215..1126
                     /locus_tag="IW819_003002"
     CDS             215..1126
                     /locus_tag="IW819_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1246068.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1279..1764
                     /gene="vanZ"
                     /locus_tag="IW819_003003"
     CDS             1279..1764
                     /gene="vanZ"
                     /locus_tag="IW819_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1246069.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2051..2380)
                     /locus_tag="IW819_003004"
     CDS             complement(2051..2380)
                     /locus_tag="IW819_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1246070.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2685..3014
                     /locus_tag="IW819_003005"
     CDS             2685..3014
                     /locus_tag="IW819_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1246071.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3004..3291
                     /locus_tag="IW819_003006"
     CDS             3004..3291
                     /locus_tag="IW819_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1246072.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3570..4133)
                     /locus_tag="IW819_003007"
     CDS             complement(3570..4133)
                     /locus_tag="IW819_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1246073.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1661 a          708 c          800 g         1548 t
ORIGIN      
        1 gtcaacaggc agttcagcct cgttaaattc agcatgggta tcacttatga aaattcatct
       61 acattggtga taatagtaaa tccagtaggg cgaaataatt gactgtaatt tacggggcaa
      121 aacggcacaa tctcaaacga gattgtgccg tttaagggga agattctaga aatatttcat
      181 acttccaact atatagttaa ggaggagact gaaaatgaag aagttgtttt ttttattgtt
      241 attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt tttctcagga
      301 aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa atagtgggac
      361 ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag gaaatctgct
      421 attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata tcgtgaattt
      481 atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata tttatatgtc
      541 aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg gtggcgttag
      601 tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc tttaccaaga
      661 aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag gtttatcact
      721 agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt ggatagaaga
      781 aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag agttaacagg
      841 aattcaatat gaaccatggc atattcgcta tgttggttta ccacatagtg cgattatgaa
      901 agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa aaaccatttc
      961 tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta aaaataccac
     1021 cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag acggtgtaat
     1081 tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat ggcggaatga
     1141 aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa cgttagcttt
     1201 ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac ttaaacaaaa
     1261 gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc tttatattta
     1321 gtgacactaa tctggttagt gttattcaaa ttacaataca atattttatc agtatttaat
     1381 tatcatcaaa gaagtcttaa cttgactcca tttactgcta ctgggaattt cagagagatg
     1441 atagataatg ttataatctt tattccattt ggcttgcttt tgaatgtcaa ttttaaagaa
     1501 atcggatttt tacctaagtt tgcttttgta ctggttttaa gtcttacttt tgaaataatt
     1561 caatttatct tcgctattgg agcgacagac ataacagatg taattacaaa tactgttgga
     1621 ggctttcttg gactgaaatt atatggttta agcaataagc atatgaatca aaaaaaatta
     1681 gacagagtta ttatttttgt aggtatactt ttgctcgtat tattgctcgt ttaccgtacc
     1741 catttaagaa taaattacgt gtaagatgtc taaatcaagc aatctgatct ttcatacaca
     1801 taaagatatt gaatgaattg gattagatgg aaaacgggat gtggggaaac tcgcccgtag
     1861 gtgtgaagtg aggggaaaac cggtgataaa gtaaaaagct tacctaacac tatagtaaca
     1921 aagaaagccc aattatcaat tttagtgctg aggaattggt ctctttaata aatttcctta
     1981 acgttgtaaa tccgcatttt cctgacggta cccccaaact aagatttcat agatagattt
     2041 tctgataact ttaaaaaaca cgaatagcgg aaaggatgtc ttcattcgct aaagagtaat
     2101 aaactacttt tccatcttga cgtgattttg ccattccatg tttttttaaa aaacgtaaat
     2161 ggtgtgacgt tgtagcgaca ctagcagtaa gtatttctgc taaatcacag acgcacattt
     2221 ccttataggt ttctaaagca tagaaaattt taattctaga aaaatcgctg aaacatttcc
     2281 ctaacacaag taagtttgaa aaatcttttt gatccaattt attttttatt ccatttactt
     2341 tttctttatg tacgacagtc acttggcaaa cttttttcat tcatctcacc tcacattcaa
     2401 acgattattt gaatgaagta tacctatttg atcactcttt tgtcaatcta ccttaattcc
     2461 aatacactat tataagcaat ttcatgtatt gaagtaaact tacttgcaag tgcgtttttg
     2521 attccaagtt aaaaacatat tattattcta cataatttcc taatcaaata aaactccttt
     2581 gaaaattgtt ctaaaggagt tttattattt tgtatttcta ctaaaaatag cgtacaatgt
     2641 aattactaag taattacgaa atgtaaaata atggaggaaa taatatgatt acaaaaacaa
     2701 gaaaacaagg aaattctatt atgttaacgg tccctaaaga attcgatgtg cccaatggtg
     2761 ttgaagtaga agctaagctt gtagagaatg gtatccttta tgagtttgta gagcctaaaa
     2821 aagaattttt tgattttagt gaagatattt tggctgatat tctttcagaa ggttatagca
     2881 aacaagagat cttaaaagaa tttagaaata gaaaaaatgc gttaacttct gcatttaaat
     2941 ctattgctga agatactgtt gcaaattcta aaccgatgac aaaagaggag ttggcagcag
     3001 aaattggctt atgagattct accttctaaa cacgtaataa agtatctaaa aaaattaaaa
     3061 gagaaaacat taaaagaaca atttttaacg ataatttatg atgagatagc agttcgtcct
     3121 cattcgggag aacaaaaaac gggtgattta tcaggaattt gggctattgg attcaagtat
     3181 gcaggtacga catatcgcgt tgcatatgaa attaaagata atgcagtgat acctatctta
     3241 ctatgtggaa cgcatgaaaa tttttatgaa caattaaaaa agattaggta agaacccaat
     3301 gtttcaactt gagaggatag acgacacttt aaaagataat ttttaacagc tgagagttgt
     3361 aataccctat aattttaaac cactaaccta tatatttatg aaaaatagaa aaaattaaaa
     3421 cgcattttaa gcctctttca aattaaaagc ttcatcctta tgcacaaaga gggcttaaaa
     3481 tgcgttattt tatattgttt tttagtaata ataaaaaaat cactctaatt ctcccataaa
     3541 tattatagat tcctaaagat taaaatagat tattccctat tgtcttttaa gtagcggtag
     3601 agtgttgcct tgctaatacc agtcatttcc gtaatttctt ttacgctata aacctgactt
     3661 ttatacaatt ttactgctcg atcaacatct ttctggttca ctcgtggacg gccaccttta
     3721 cgacctctcg cacgtgcact tttcaaacct tctttggtac gttcaacaat taaatcacgt
     3781 tcgaactgac taaatgcttg gaagacagtc atcattaagc gtccgtgagg cgtgctggta
     3841 tcgaagtttt ctttcaagct caccagctta acatggtgtt cttcaaaata ggtaacaagg
     3901 tcaatcaaat cctttgtact acgacccaat cgagaaaaac tttctacaat taccgtatct
     3961 cccggacgaa ttttatcttt taaacgattt agttggggac gaaccgcttt ggttcccgtc
     4021 attttctcag ttaggatttc attacaattt tgttcattga gtaagtctaa ttgacgattt
     4081 agttcttgtg aacgtgtact cactcgggca taaccataaa tgtatccact catgcttttc
     4141 ttcctttcgt actttaattc tggcttgatt gtatcataaa agggagttat agatacatag
     4201 ttaatgcaac aagttatgat acaaaatatg acctctttta tttaataaaa gaggtcattt
     4261 cattttgtct cataatcgat tgtttttgat acgagtgtgt atacgaaata aacaaaaaaa
     4321 tcttaagtaa atttctagat acctaattta atcctaacta gtgtatccta aagttaataa
     4381 cgagtttatc acaatgaaaa ttataaaatg ctcccatatt agatataaca tgcaatattt
     4441 gaattagaac tacaaaattt attcgtttta taaagtaaat aatgttgaga tactaaggaa
     4501 tagaatatta taaggaattg agctgaatgt attggataaa aaaagaataa ctttattaga
     4561 gatggactat aaaaatgctc gtaattttct tctgaagtct gaaagctatt tgaatgctca
     4621 gctaccagaa tatattagtt ttgacgaaac tttaaaatct gctaaaaaag ttttaacttc
     4681 gccgtttgta taattaagcg agacaaataa aaaagcc
//
LOCUS       DACEPW010000434         3538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.guided.224, whole genome shotgun sequence.
ACCESSION   DACEPW010000434 DACEPW010000000
VERSION     DACEPW010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.guided.224"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            253..1467
                     /locus_tag="IW819_003008"
     CDS             253..1467
                     /locus_tag="IW819_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1246074.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1498..1716
                     /locus_tag="IW819_003009"
                     /pseudo
     CDS             1498..1716
                     /locus_tag="IW819_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2135..2608
                     /gene="dfrF"
                     /locus_tag="IW819_003010"
     CDS             2135..2608
                     /gene="dfrF"
                     /locus_tag="IW819_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1246075.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2735..3391
                     /locus_tag="IW819_003011"
     CDS             2735..3391
                     /locus_tag="IW819_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1246076.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
BASE COUNT         1169 a          512 c          752 g         1105 t
ORIGIN      
        1 gagccgtatt ctttattcct tatgaaaaaa tatctttaaa aataaaaaaa gacgaaaata
       61 aacggttttc tataaaaaga agaccgttta ttttttgtct ttatttgtgt gaaaaacgaa
      121 aaagagcaca acacatcaag cttttattaa ttgtctgtct tttataaatg caagtattaa
      181 ttcaaaaaaa cagaagaaaa aaacaactac accttctttt atactcgtaa tcgtagaaag
      241 gaggcgaaaa aaatgaatac attacctatc aatacaggag atacagcatt catgatttta
      301 tgtacagcaa tggtttgcct gatgactcct ggtctagctt ttttttacgg cggattagca
      361 cggaagaata atatactaac gatcatgtct caatcttttc tctccgttgg gataacaacg
      421 attatgtggg tatttggtgg attcggattg gcctttggac gggatatcgg cggtgttatt
      481 ggaaacccgg cagatttctt tttgatgaga catgttacaa gcttccccaa catgtttcat
      541 ggtgcaacga ttccattttt aatgttcttt ctttaccaac tgatgtttat cgtgattacc
      601 gttcctttga tgacaggagc ttttgcaggg agattgaatt taaaaggcta tattatactt
      661 gttatttttt ggaatctgct gatttatttc cctgtttgcc actggatatg gggtggcgga
      721 tttttggatc agatgggatt tagagatttc gctggtggag cagtcatcca cacgacagct
      781 ggattcggct ctttagcatg tatcctcaca cttggtcaaa gaaagattcc tttaggagaa
      841 agaagtcagc atagcagtct tatggcagct gctatcggta caggactgct ttggtttggc
      901 tggttcggtt ttaattccgg tggtgcactt cgagcagatg aacaagcagt caatgcattt
      961 ggttcaacat tcatcgctct tgcatttgcg atgattactt ggctgatcat tgcaaaagta
     1021 aaaggaaatg gctttgattt tgtcgacgta ctaacaggat ccgttgctgg attggcagcc
     1081 attacgcctt gtgccggtta tgtagaagca aaaagtgcca tgctgattgg gatcattgcg
     1141 ggtatcgtct gtcatgctgc tgttgatttt cgtaaaaaga aacaatggga tgatgcactt
     1201 gatgtgtggg gtgttcatgg catgggcgga ttcacaggta cgattctgat agggatcttt
     1261 gcatctaaca gccatttact aacgaatcca agcagctggt acttcttagg gattcaagtg
     1321 attggtgtca tcattactgc cgtgtatgcc tatgtgctga caacagtgat tttaaaatcg
     1381 gcgatgcact ttacaacgat cactactaca aaagaagaac aagaagaagg attggacttt
     1441 ttcttctttg gcaacaaact caattgaaac aaattcaaat agaaaagagg aagattcatg
     1501 caacaaagca aaacgaaaat tcctgcaaaa aaattaacgt tctttggctt tttggctatg
     1561 accatctcaa tggtcgtttc attatatgaa tatcccacat tcgcgacctc aggcttcagc
     1621 ttagtatttt tcttgttatt aggcggtttg ctatggttta tccctgtggc attgtgcgca
     1681 gcagagatgg caacggtgaa aggatgggaa aaaggtgttt cccaaacaaa aagatattaa
     1741 gaaacagccc atgtttacct gcttgcaaac aaaagaatat tgctactaga ctaagagata
     1801 ctacataagt gtgttggtat ctcttttttg ttgccgtgaa acagcagact atttcatttc
     1861 tgcttgatta ccttaaaact tattagtcta tggtttgtca agggcttgtt gcgtgagaaa
     1921 tgctgaatat caccctttac aaaacatagg ctgataagta acttttcaaa agcagaaaag
     1981 aaatggcaat gcttataaca atatgatata atgcagatga agacaaaaaa ggaatttgtc
     2041 gagctggtta gttcgagata attttgagtt tgttaagatg tggtgcactt gatggaatca
     2101 caggtacgac atcatattat ggaggatgaa atagatgata ggtttgattg ttgcgaggtc
     2161 aaagaataat gttataggca agaatggtaa tataccatgg aaaataaagg gagaacaaaa
     2221 gcaatttaga gagttaacaa cgggtaatgt ggttattatg gggcgaaagt cttatgaaga
     2281 aatcggtcat ccgttgccta atagaatgaa tattgttgtt tccaccacaa cagagtatca
     2341 aggagataat ttagtttcag ttaaatcatt agaagatgca ttattattgg ctaaaggacg
     2401 agatgtatac atatctggtg gatatggact atttaaggaa gctttgcaaa tagtagataa
     2461 aatgtatatc acagaagtag atttaaatat tgaagatgga gatacattct ttccagaatt
     2521 tgatatcaat gattttgaag ttttgatagg ggaaacactt ggtgaggaag tgaaatatac
     2581 gagaacattt tatgtaagga aaaaatgaat tgagtaaatt ttggatttag ggtgttttca
     2641 taaatatatt gctttttatg catatataaa ttgttgtaag acaaattcca gtttgtatgg
     2701 ggcgataaaa tcagaatttg agagaggatt gaatatgaat aaggaatggt ctgaacttaa
     2761 taaaacaatg caggcacaaa taaaaaagaa agatacttat aagaggggta ttgatacttt
     2821 acttacttta cgaagtcagt taatacaaac cttagtatct ttcaaagaag aattatgcag
     2881 agaagatttt aactcaatcc ccttcataaa cgctgatggt tatcatagta agacgattgc
     2941 ttattctatt tggcatattt tccgcattga agatatagtt gtgcatacag tgataaatga
     3001 agatgaacaa gtattttttg caggcaatta tcaagagcgt atcaattcac ctatcattac
     3061 aacaggaaat gaactcatga aacagcagat tgcagacttt tcaaaacagc ttaatctgga
     3121 agaattatat ttatatattt tcgaagtatg ggaaagcact gagaaaatgc ttgaacggtt
     3181 atcttatgat gaattgaaaa gaaaaatacc gaaagaaaga aaaggatact tagaatcgtt
     3241 gaatgtagta aacgacaatg aaaaagcaat ttggctgatt gattattggt gtaataaaga
     3301 tatttgtgga ctaattcaaa tgccgttttc aagacattgg attatgcaca cagaagcctg
     3361 tttgcgtata aagaacaaga tacattcata ggcaaaaatt tcagtttgta gaactaagga
     3421 aattggaatt taaggaggaa taaagtatgg ttagtgattt tataagagca gcatttccat
     3481 ggattttaat gggtttattt gtagcaatca gttgttccct tatgagtaaa aaagagaa
//
LOCUS       DACEPW010000435         2794 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.guided.260, whole genome shotgun sequence.
ACCESSION   DACEPW010000435 DACEPW010000000
VERSION     DACEPW010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.guided.260"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1213
                     /locus_tag="IW819_003012"
     CDS             245..1213
                     /locus_tag="IW819_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1246077.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IW819_003013"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IW819_003013"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1246078.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..>2794
                     /gene="vanX"
                     /locus_tag="IW819_003014"
     CDS             2243..>2794
                     /gene="vanX"
                     /locus_tag="IW819_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HAQ1246079.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWH"
BASE COUNT          823 a          539 c          687 g          745 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcac
//
LOCUS       DACEPW010000436         1834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.guided.324, whole genome shotgun sequence.
ACCESSION   DACEPW010000436 DACEPW010000000
VERSION     DACEPW010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.guided.324"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1747
                     /gene="lsa"
                     /locus_tag="IW819_003015"
     CDS             245..1747
                     /gene="lsa"
                     /locus_tag="IW819_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1246080.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
BASE COUNT          650 a          318 c          396 g          470 t
ORIGIN      
        1 tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt gcattgaaaa
       61 attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa ggaagcagtg
      121 tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt gtgtatccaa
      181 cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta cgaggtgatg
      241 gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc aaggcacatt
      301 attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac ttatcggacg
      361 aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac cttatcaagg
      421 gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag ataaagaacg
      481 tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa tcgaaagaga
      541 gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca cactatcggg
      601 gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc atttcccgtt
      661 aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag cggcttattt
      721 gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta tcgatgaagt
      781 agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag ggaatttctc
      841 tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa atgaaaaatt
      901 gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat ggtctcgttc
      961 ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat ctagacgagt
     1021 gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca aagcaatcgt
     1081 gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata tcgaatatat
     1141 cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa gcgtagaaga
     1201 tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa tcgagcctca
     1261 tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta tccattatct
     1321 tctgggggca ttcaacggca aggttatagg agaaaaatac cagccaaaac atctgagcat
     1381 tagttatgca agccaaaatt atgaagacaa tcgaggaacg ttggcggaat ttgcagagaa
     1441 aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga tggaaagaga
     1501 tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag tggaattggc
     1561 taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga attatttgga
     1621 tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg ccatgttact
     1681 agtggaacat gatcaaacct tcctggataa agtatctact gagattattt ctcttgagaa
     1741 aatctaaacc ttgtaatctc aaagacaaca aggtatggtt aaggagaact ttgtcaacga
     1801 aggagagaat cacatgcttt atgtaacgag acac
//
LOCUS       DACEPW010000437         1834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.guided.325, whole genome shotgun sequence.
ACCESSION   DACEPW010000437 DACEPW010000000
VERSION     DACEPW010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.guided.325"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1747
                     /locus_tag="IW819_003016"
     CDS             245..1747
                     /locus_tag="IW819_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1246081.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
BASE COUNT          647 a          320 c          398 g          469 t
ORIGIN      
        1 tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt gcattgaaaa
       61 attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa ggaagcagtg
      121 tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt gtgtatccaa
      181 cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta cgaggtgatg
      241 gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc aaggcacatt
      301 attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac ttatcggacg
      361 aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac cttatcaagg
      421 gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag ataaagaacg
      481 tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa tcgaaagaga
      541 gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca cactatcggg
      601 gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc atttcccgtt
      661 aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag cggcttattt
      721 gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta tcgatgaagt
      781 agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag ggaatttctc
      841 tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa atgaaaaatt
      901 gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat ggtctcgttc
      961 ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat ctagacgagt
     1021 gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca aagcaatcgt
     1081 gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata tcgaatatat
     1141 cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa gcgtagaaga
     1201 tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa tcgagcctca
     1261 tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta tccattatct
     1321 tctgggggca ttcaacggca aggttatagg agaaaaatac cagccaaaac atctgagcat
     1381 tagttatgca agccaaaatt atgaagacaa tcgaggaacg ttggcggaat ttgcagagaa
     1441 aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga tggaaagaga
     1501 tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag tggaattggc
     1561 taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga attatttgga
     1621 tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg ccatgttact
     1681 agtggaacat gatcaaacct tcctggataa agtatctact gagattattt ctcttgagag
     1741 aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact ttgccaacga
     1801 aggagagaat cacatgcttt atgtaacgag acac
//
LOCUS       DACEPW010000438         1655 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E366
            SAMN07141450-rid9868213.guided.341, whole genome shotgun sequence.
ACCESSION   DACEPW010000438 DACEPW010000000
VERSION     DACEPW010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141450
            Sequence Read Archive: SRR5811356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740686.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740686.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:36:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,994
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 25 of 54
            Pseudo Genes (incomplete)         :: 25 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141450-rid9868213.guided.341"
                     /isolate="E366"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Aug-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..932
                     /gene="vanA"
                     /locus_tag="IW819_003017"
     CDS             <1..932
                     /gene="vanA"
                     /locus_tag="IW819_003017"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HAQ1246082.1"
                     /translation="KYEPLYIGITKSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGL
                     LVKKNHEYEINHVDVAFSALHGKSGEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLT
                     YIVAKNAGIATPAFWVINKDDRPVAATFTYPVFVKPARSGSSFGVKKVNSADELDYAI
                     ESARQYDSKILIEQAVSGCEVGCAVLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKG
                     SENAVITVPADLSAEERGRIQETAKKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLP
                     GFTSYSRYPRMMAAAGIALPELIDRLIVLALKG"
     gene            938..1546
                     /locus_tag="IW819_003018"
     CDS             938..1546
                     /locus_tag="IW819_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1246083.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          486 a          321 c          409 g          439 t
ORIGIN      
        1 aaaaatacga gccgttatac attggaatta cgaaatctgg tgtatggaaa atgtgcgaaa
       61 aaccttgcgc ggaatgggaa aacgacaatt gctattcagc tgtactctcg ccggataaaa
      121 aaatgcacgg attacttgtt aaaaagaacc atgaatatga aatcaaccat gttgatgtag
      181 cattttcagc tttgcatggc aagtcaggtg aagatggatc catacaaggt ctgtttgaat
      241 tgtccggtat cccttttgta ggctgcgata ttcaaagctc agcaatttgt atggacaaat
      301 cgttgacata catcgttgcg aaaaatgctg ggatagctac tcccgccttt tgggttatta
      361 ataaagatga taggccggtg gcagctacgt ttacctatcc tgtttttgtt aagccggcgc
      421 gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc ggacgaattg gactacgcaa
      481 ttgaatcggc aagacaatat gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg
      541 aggtcggttg tgcggtattg ggaaacagtg ccgcgttagc tgttggcgag gtggaccaaa
      601 tcaggctgca gtacggaatc tttcgtattc atcaggaagt cgagccggaa aaaggctctg
      661 aaaacgcagt tataaccgtt cccgcagacc tttcagcaga ggagcgagga cggatacagg
      721 aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg tctagcccgt gtggatatgt
      781 ttttacaaga taacggccgc attgtactga acgaagtcaa tactctgccc ggtttcacgt
      841 catacagtcg ttatccccgt atgatggccg ctgcaggtat tgcacttccc gaactgattg
      901 accgcttgat cgtattagcg ttaaaggggt gataagcatg gaaataggat ttactttttt
      961 agatgaaata gtacacggtg ttcgttggga cgctaaatat gccacttggg ataatttcac
     1021 cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta gggacatacg agttggctga
     1081 atcgcttttg aaggcaaaag aactggctgc tacccaaggg tacggattgc ttctatggga
     1141 cggttaccgt cctaatcgtg ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa
     1201 taacctgaca aaggaaagtt attatcccaa tattgaccga actgagatga tttcaaaagg
     1261 atacgtggct tcaaaatcaa gccatagccg cggcagtgcc attgatctta cgctttatcg
     1321 attagacacg ggtgagcttg taccaatggg gagccgattt gattttatgg atgaacgctc
     1381 tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc
     1441 catcatggaa aacagtgggt ttgaagcata tagcctcgaa tggtggcact atgtattaag
     1501 agacgaacca taccccaata gctattttga tttccccgtt aaataaactt ttaaccgttg
     1561 cacggacaaa ctatataagc taactctttc ggcaggaaac ccgacgtatg taactggttc
     1621 ttagggaatt tatatatagt agatagtatt gaaga
//
